LOCUS       DACECZ010000001       137801 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.001, whole genome shotgun sequence.
ACCESSION   DACECZ010000001 DACECZ010000000
VERSION     DACECZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 137801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 137801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..137801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.001"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            67..231
                     /locus_tag="IWO35_000001"
                     /pseudo
     CDS             67..231
                     /locus_tag="IWO35_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
     gene            479..697
                     /locus_tag="IWO35_000002"
                     /pseudo
     CDS             479..697
                     /locus_tag="IWO35_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(826..2034)
                     /locus_tag="IWO35_000003"
     CDS             complement(826..2034)
                     /locus_tag="IWO35_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEnfa110 family transposase"
                     /protein_id="HAQ0315095.1"
                     /translation="MNCVGIDVSKGKSMIAVMRPFGEVVVSPFEVRHTANELSELAGL
                     LKSLDGETRVVMESTGNYHAPVAWLLHDAGLYVSVVNAMLVHDYGNNSLRRAKTDKKD
                     AVKLANYGLDHWLTLPRYIPEEDTRLMLKICYRQYQQYSKVQTMLKNNLISLLDTTFP
                     DANRLFTSPPRADGSEKWVDFVATFWHCECVCGRSEKAFTTQYQKWCRKHGYNFSEDK
                     ALDIYASACRHFGVIPKTDTAKLLVEQAISQLQTTSSALAALKQEMQSLAASLPEYPV
                     VMGMFGVGPTLGPQLLAEIGDVRRFHSKKALVAFAGIDAPPYQSGQIDVRSRSISKRG
                     SASLRRTLFLVMSVILQCAPIDEPVYQFMDKKRSEGKPYRVYMMASANKFLRIYYASV
                     KAYLESLEHD"
     gene            2324..3289
                     /locus_tag="IWO35_000004"
     CDS             2324..3289
                     /locus_tag="IWO35_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0315096.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            3311..3523
                     /locus_tag="IWO35_000005"
     CDS             3311..3523
                     /locus_tag="IWO35_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ0315097.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            3598..4074
                     /locus_tag="IWO35_000006"
     CDS             3598..4074
                     /locus_tag="IWO35_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAQ0315098.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            4071..4571
                     /locus_tag="IWO35_000007"
     CDS             4071..4571
                     /locus_tag="IWO35_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HAQ0315099.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPS
                     APVRAV"
     gene            5131..6948
                     /locus_tag="IWO35_000008"
     CDS             5131..6948
                     /locus_tag="IWO35_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0315100.1"
                     /translation="MRNPIHVLKSLEEKASVSNYKYERLYRNLYNPEFYLLAYANIAK
                     SQGSMTQGVDGQTLDNMSLPRINRIIESIRNRTYQPKPAKRKYIPKKNGKLRPLGITS
                     TDDKLVQEVVRMILEAIYEPTFSNNSHGFRPKRSCHTALTQVKKNFTGVTWIVEGDIK
                     ACFDNFDHHVLVELLRKRISDEAFIGLIWKFLKAGYMEQWQYNCTYSGVPQGSGISPI
                     CANIYLSELDNYMQEYKEKYDCEPERRRTTREYERASRRYRKARKALMGAEKSTPELV
                     KEFKDSRRKKMNQHYYNPFEEGFKKIQYNRYADDFVIGVIGSKKDAEKIKEDVKIFLQ
                     EKLHLEMSEEKTKVTHSSKPVRYLGYDFKVIHSKNMKRCKNGDMKRVWYGKVFLYMPK
                     EKWIKKAMERGAIQVKRNNDTGKEMWRPMPRKDLMNRSDAEIVSTFNSEIRGLYNFYR
                     IAENVGALHKYYYMVRYSMLKTLAGKHRTNVSVIKKRHMVNGVLRIPYDTTKGRKYCE
                     FYHDGFRKHSDGYDNVADVMPSYRKYDSRHTIVNRIKAGVCEICGEHADYLCMHHVRT
                     LKSLKGRDIFEQKMLKMRRKSLALCPDCFELLHETKESR"
     gene            7057..8382
                     /locus_tag="IWO35_000009"
     CDS             7057..8382
                     /locus_tag="IWO35_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ0315101.1"
                     /translation="MQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTIN
                     FSKSMKYNPFVYIHSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIW
                     YEAPAEEMNFSTLLEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLL
                     SAGKTRSSILISCGARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFN
                     FILAMLQSQLINLLCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREI
                     SASIILQSQSQLKAIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTS
                     ENRGSQTSHGLNYQKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYK
                     YLADADKKNTFDVDRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            8459..8620
                     /locus_tag="IWO35_000010"
     CDS             8459..8620
                     /locus_tag="IWO35_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315102.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKHLMSSM"
     gene            8661..9041
                     /locus_tag="IWO35_000011"
     CDS             8661..9041
                     /locus_tag="IWO35_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HAQ0315103.1"
                     /translation="MTISCKAKGDTMKIEYTKVGDYYLPNLYYPEETRPIGLWGMLRK
                     EYLKEHKSGTYTYLLLTARLDSYLADLNEQAQERFELIETQMRSAEGVSEELKRQNPM
                     EWLCHCNNIRNRAAEIIKQELIYV"
     gene            9056..9337
                     /locus_tag="IWO35_000012"
     CDS             9056..9337
                     /locus_tag="IWO35_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315104.1"
                     /translation="MRRELIESYYEEYQRELERFLESDPEMFTLEAEIHHYIEILEPV
                     LRDGGQEMWQTLDRIISAKNAMEGIVAKEMYIRGFLDYERLVCEKDSKI"
     gene            9596..10378
                     /locus_tag="IWO35_000013"
     CDS             9596..10378
                     /locus_tag="IWO35_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAQ0315105.1"
                     /translation="MKVKNADEFYKILERKAIYPVFQPIVNLQTGDVAGYEALSRIDR
                     HDTTLMISDLFVIAEQVGCVWKLEKLCRNKALKAAANKPEHAKLFLNVDGNIIQDKSF
                     IQGFTNRKAAKAGVPSCDIVFEITERSDIENYQILQQIMKHYADQGYEIALDDVGAGY
                     SGLNRVVNTSPNYLKVDIELVRDIQKHKKKEIMMEFLLHYCNETGAILIAEGIETEAE
                     LECLCRLGVHYGQGYFLGRPDRTFKNVRNEAMLVLRRLNNKS"
     gene            10551..12269
                     /locus_tag="IWO35_000014"
     CDS             10551..12269
                     /locus_tag="IWO35_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAQ0315106.1"
                     /translation="MNENVKVVFGLIGGLALFLYGMNSMSDALQKAAGERMKKILGFL
                     TRNPIMGALAGALVTAVLQSSSATTVMVIGFVSAGLMSLPQAISVIFGANIGTTMTAQ
                     LMAFKISNYIYPIIFVGFILNFVSKKEKVKNIGMVIFSFGLLFEGIEIMGEVMKPLAG
                     SPVFVDLMGKVSSIPVLGVVLGAVMTLVVQSSSATIAVLQNFASQAGPDGVSSVIGLT
                     GAIPILLGDNIGTTITALLASIGQSKNAKRTAIAHSIFNISGSCVFIFLVPWFAKFVQ
                     FISPKGNEIDVISRQIANAHTTFNIVCTLVWLPLIPLMVKIVTTIIRGNDKTEKAAFE
                     PKYLDMKVIEQPAAAMVLVSKELNRLGELAESLLSDLKTAIVADGDSKTHGSFIENLE
                     IVHQLQDSVTEYITRLFASGNLTEQQSEQTAGLLYVNNSIQRIADRCEDIDQICEKVN
                     NNGKRLTSEASSEMEDCIDIIQQLLKKAMEAIRKGDAVVAETVFKNKKKMHKAEKKYS
                     KAHLNRVKNEVCDASMTRYFSGIMYNFDRMADNCVSIAEEACDNVAFINLDEETGKSM
                     MERGAV"
     gene            12271..13125
                     /locus_tag="IWO35_000015"
     CDS             12271..13125
                     /locus_tag="IWO35_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315107.1"
                     /translation="MVKKKNIRKSALLLIMIMLATGITACGTEEEPLPDLSAMGAVTA
                     VSREEGSGTRAEFENLLKLPESDTGIVVDSTEKVLKKVEEDKNAVGYVAYSSATDTNG
                     KILQINGVLPSEKTIDNNSYPLCRDYYLAYNGELTDVEQDFLTYVKSKGQDIVKQYCI
                     QADSTTTFLSDKSEGKILIEGSTSMEPMVKALADDYQKQNPNAEIEVKATDSSRGITA
                     VISGECDFAMSSRELKDYEAELLETKVIGKDAIAIVINEENPLENLTIKQLTGLYNGT
                     YKNWDDLS"
     gene            13801..14121
                     /locus_tag="IWO35_000016"
     CDS             13801..14121
                     /locus_tag="IWO35_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315108.1"
                     /translation="MPGKEQFMTELKMKEMQLINWFGCPNLIYTRERIHLVAMLTTDE
                     EMKKNLYRVCRFLAREEIAYRYPIMYEMIRKIFSPDDTNPPEACAMFMPDDETDDEEY
                     AMAA"
     gene            14145..14555
                     /locus_tag="IWO35_000017"
     CDS             14145..14555
                     /locus_tag="IWO35_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0315109.1"
                     /translation="MNKVVLMGRLTKNPEIKYAGKDNDMAVARYTLAVNRRYKRDGEQ
                     EADFISCVTFGKSAEFAQKYLHKGMRIVIGGRISTGNYKDKDGKTIYTTDVIVEEHEF
                     AQNKDNGVGADSSETPKTDKDGFMEAPEGEVPFD"
     gene            14702..14908
                     /locus_tag="IWO35_000018"
     CDS             14702..14908
                     /locus_tag="IWO35_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0315110.1"
                     /translation="MGVSYNRLWKMLIDKNMKRIEMQYLTGISGNILARMGKNQYVSM
                     ETIEKICKKLDCTVDEMLEFTDDE"
     gene            15255..15776
                     /locus_tag="IWO35_000019"
     CDS             15255..15776
                     /locus_tag="IWO35_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0315111.1"
                     /translation="MIRKAILKDVNSIEDTYNEHFQYELNHTAFTVFKKGVYPTKDDA
                     ERAIYAGALFVYEENGTIVGSIIIDKVQPIEYATIPWKEKLSEDEVMVIHLLMVRPSM
                     SGKGIASSLIKFATELAQKNSCRALRLDTGSQNIPALSLYQKNGFEIIASASKKVGDV
                     IAHKNHLFLEKIL"
     gene            15834..16259
                     /locus_tag="IWO35_000020"
     CDS             15834..16259
                     /locus_tag="IWO35_000020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0315112.1"
                     /translation="MIRELRKVDINKVAEIWLDTNIKTHYFISAQYWKSNFELVKELL
                     LQATVYVYEDKQEIQGFIGLSNEYIEGIFVSAEMQSQGIGKILLNYVKGKRNKLILNV
                     YQKNTRAISFYQREGFEIQYSGLDEATGEKDYVMAWQQK"
     gene            16438..16668
                     /locus_tag="IWO35_000021"
     CDS             16438..16668
                     /locus_tag="IWO35_000021"
                     /inference="COORDINATES: protein motif:HMM:NF025559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HAQ0315113.1"
                     /translation="MQDDDSGFTYEISRCLYFDTCKEHGYAEFCKVFCNHDWYAYGVL
                     KHHSRFIRKSTIAEDGTVCHDTIEKVTEKHFL"
     gene            16702..16884
                     /locus_tag="IWO35_000022"
     CDS             16702..16884
                     /locus_tag="IWO35_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylan 1,4-beta-xylosidase"
                     /protein_id="HAQ0315114.1"
                     /translation="MAKEERFEKIYSQGALTTTEIWVDKETGVNYLYHACGYSGGLTP
                     LLDRDGKPVISQAINR"
     gene            16997..18508
                     /locus_tag="IWO35_000023"
     CDS             16997..18508
                     /locus_tag="IWO35_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315115.1"
                     /translation="MRRSGWTKWLIVIPAMLGIVLVTYVRYTTDIWGFGAFICQLIAI
                     LELAYGLRIAMLAQNRKKSYRLTPEERHEYARYLYEKQYHRYPAVANQMLLVMARMSV
                     LLNNYERAAQELADIRIDKFNPAQLKLYYYLKVVTAMAAGDATGIQESQMCYAGIPDT
                     KCTYPSKAELAMWIEQRAAAQMAEALKKAVPDKKEHPIRISIITLVLAYSTFFYGLWY
                     GIKRDMGYEVRYRFAEISITVITISLAVLLIWGIVSLYRHNCQALSIQFGKNKVALII
                     VYGAAAIFALLIIGMSGLSVVFGMDGTETVTGCDSKYTYITVQEDYGGERRYRTNNPF
                     VMQSMDGLWWSSDTIDEEDNQEEDTTELPDLDTENSTDSEDIQSGNTIEDYETEGQHD
                     GLTIQNAMLAVYNYLQEQNTLQNMAFSYTANAKGEVYAVVSETQETKNGNTVNVRYCL
                     YDNGSKTDADGNSCEELVLEKVYPDGNYETELVDFYLVNADTMQVTDEQKNTW"
     gene            18831..19055
                     /locus_tag="IWO35_000024"
     CDS             18831..19055
                     /locus_tag="IWO35_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HAQ0315116.1"
                     /translation="MSEKTEITFMQTRLIRLASEEWHLPVEQIIHLFKEADVLGYIEK
                     CYGIFHCEGDEAVLEDITEFLQRKGIEISA"
     gene            19048..19614
                     /locus_tag="IWO35_000025"
     CDS             19048..19614
                     /locus_tag="IWO35_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HAQ0315117.1"
                     /translation="MLELKDGFVLYHGSYCEVKEPDLAKCAKRKDFGQGFYLTTSKEQ
                     AESFLRTSIAKAIATGTIEEGQKFGYISTFEFKLSENLETHIFENADVDWLHCIAAHR
                     KKKMFIEVEREMAKYDIIAGKIADDATNATLTAYLAGAFGTAGDKVADDFCIRQLLPN
                     KLKDQYCFKTEAAIECLKFVKGEKIWLK"
     gene            19602..19832
                     /locus_tag="IWO35_000026"
     CDS             19602..19832
                     /locus_tag="IWO35_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315118.1"
                     /translation="MAEVTIPKEKMNYTIDLLITMVTDEIAEETGKDRNEVLTDFLCS
                     KTGKALYDEKTKLWCTGPAYIAELYMEELKKS"
     gene            20033..20398
                     /locus_tag="IWO35_000027"
     CDS             20033..20398
                     /locus_tag="IWO35_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HAQ0315119.1"
                     /translation="MSRAKNPKYESMSLEELKANMEKSRQELEAAIHHKNILEQRKKL
                     VERRERSHRLIVKGAEFEKVFPLSRDLEQEDVQDMMNQLQNSSYNKSIVRQAHIAALH
                     KDQQQIAEAVKKTEKGDDS"
     gene            20398..22125
                     /locus_tag="IWO35_000028"
     CDS             20398..22125
                     /locus_tag="IWO35_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HAQ0315120.1"
                     /translation="MALYHFSVRNVSRGKGQMVVASAAYISGQRIFDSYYNKIHDYTS
                     KSGVIFTEILTPEYVPERLTDRETLWNEVEHVERNNKAQLAYSFDIALQNELTLEENI
                     RLAKEFCQEQFVARGMIVDLAVHEGKAENEEEPDNPHFHVLVPIRPFTEEGIWGNKQR
                     REYILDEDGNRVKDAKGKDMFNAVSTTGWNDPELLKEWRRAWTEKVNGKFRECHMASR
                     IDHRSYKEQGIDLIPTIHEGYEVRAMEKKGIKTVIGELNRAIRQFNQMFISLKESIQW
                     MKTAYEEMKAELDRRQNPTLLESLQDYYDKKTQGRPPLPNFYAEMKQRGKNLSNLQEF
                     AKSISYLQTHQIETMDDLQERIEELNGVVSVSKKEISEKRKQLKELENLEKMAEVIKT
                     NQPLIDEYNHFYFQKRRERYYQQHKKEINYYRKCERELKQHLDKNGKVPTARWKREKE
                     ELQAGIEELKADNQPYQEELTFVKKVQSCADIARRDREMAEADTSGRSEEKREKQVKF
                     PTFYTVQAKEILEENGKAEQQPLNQSEQNPEKKTSLLRKLDEKKKECAERDAKQQAVK
                     KKRNYDMSL"
     gene            22155..24071
                     /locus_tag="IWO35_000029"
     CDS             22155..24071
                     /locus_tag="IWO35_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0315121.1"
                     /translation="MSKMQYTEEQIIRANQTDLVSFLSAQGEQLGKSGKEYRWKKHDS
                     VTVSGNRWYRHSQGRGGYPVDFVMEFYNATFPEAVKMLTGEEGEGRNSTCPAPSPDFR
                     LPEKEENNDRIIRYLTENRGIEKNMVEEWIGSGDIYEEKKHHNVVFVGRDADGIPRYA
                     HCRGTGETKYRGDVAESDKSYGFCHRGTDNQLFVFEAAIDLLSFIQLFPKDWKKRSYL
                     SLGGISSAALMAFLSERPQITSVFLCLDNDHAGNEASEKLAIEIPDGYSVIRLKPSRK
                     DWNEILCDKNADRKKSIIETVTMKVPEKEELVPMLCYEDIEQTSVEWLWFPYLPFGKL
                     TIIQGNPGEGKTYFAMMLTAACTNRKTFPNMEEIEPFNVIYQTAEDGMGDTIKPRLVE
                     AGADLSRVMVIDDTEEALTLSDDRIEKAIRQNQVRLLIIDPVQAFIGADVDMNRANEV
                     RPVFRKLGMIAEKTGCAIVLIGHLNKSSGTQSTYRGLGSIDIMAAVRSLLFIGKVKKD
                     PTTRVLIHEKSSLAPPGETMAFKLGDEEGFRWVGAYEISADDLLDGKEGKPTETKLQR
                     GTKLIYELLADGNAVTIRELDEKAKAQGISQRTMREARSRMKEELDYRMNEKQENTIR
                     LKKQGRMGDGRILE"
     gene            24756..24938
                     /locus_tag="IWO35_000030"
     CDS             24756..24938
                     /locus_tag="IWO35_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315122.1"
                     /translation="MAVKKKIINIRVYFDGDQDAKEVFAEVIAQKIRDTKTKDKLAIM
                     KDKQYNDYEFSNLKSA"
     gene            24965..26662
                     /locus_tag="IWO35_000031"
     CDS             24965..26662
                     /locus_tag="IWO35_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0315123.1"
                     /translation="MNNRYMSNNIERNATWRVGVYCRLSKDDELTGESASISNQRDIL
                     VNYCQSQGWQVVDVFQDDGYTGLNTDRPDLQRLLKACEKGLVNLVITKDQSRLGRNHV
                     QTGFLMEEFFPKHGVRYIALYDNVDTFAGDNEIAPFKNVLNEMYSRDISKKVHASYHL
                     QATKGKFTGVVPPLGYQKDPEEKGHLLIDDETAPIVRKIFQWAVDGHGINFISRELER
                     QQIPCPSWWHRKRGLRTYYTKWEKKDPENGKYVWDESYLKSLLMNPVYCGDMASQKRY
                     YRFKIGNQGDKAPEDWITVRDTHEAIIPQDVFDLVQAKMKGRKRQTEQGEYSMFAGLL
                     RCAECGGALTLKKTHTKDQHEVYTCSTYIHKGKAHCTQHRVDADDLYDAVLTRIQECA
                     KAVTGDGTELENRVKELCEEDTQGHRESLEKLVSKQKDRLETLDRLIAKLYDDLINDK
                     ITESVFDKMLEKTQKEQTDIKKELFQNESVLNTEEKLDAQSQQWIDDISEYADIKELD
                     ANLLNRLISKIVISEPQEKDGTENYCRTPETVTMEIHFNLKPIPELGTIERGSGSHK"
     gene            26687..26818
                     /locus_tag="IWO35_000032"
     CDS             26687..26818
                     /locus_tag="IWO35_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315124.1"
                     /translation="MLIFSRLISSKYFLIKDIKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            26910..27773
                     /locus_tag="IWO35_000033"
     CDS             26910..27773
                     /locus_tag="IWO35_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315125.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            27804..28346
                     /locus_tag="IWO35_000034"
     CDS             27804..28346
                     /locus_tag="IWO35_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAQ0315126.1"
                     /translation="MFISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            28360..28782
                     /locus_tag="IWO35_000035"
     CDS             28360..28782
                     /locus_tag="IWO35_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAQ0315127.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            28712..31111
                     /locus_tag="IWO35_000036"
     CDS             28712..31111
                     /locus_tag="IWO35_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAQ0315128.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            31143..33134
                     /locus_tag="IWO35_000037"
     CDS             31143..33134
                     /locus_tag="IWO35_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAQ0315129.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            33157..33408
                     /locus_tag="IWO35_000038"
     CDS             33157..33408
                     /locus_tag="IWO35_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAQ0315130.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            33398..34627
                     /locus_tag="IWO35_000039"
     CDS             33398..34627
                     /locus_tag="IWO35_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAQ0315131.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            34624..36705
                     /gene="topB"
                     /locus_tag="IWO35_000040"
     CDS             34624..36705
                     /gene="topB"
                     /locus_tag="IWO35_000040"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0315132.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            36854..40774
                     /locus_tag="IWO35_000041"
     CDS             36854..40774
                     /locus_tag="IWO35_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAQ0315133.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            40775..41719
                     /locus_tag="IWO35_000042"
     CDS             40775..41719
                     /locus_tag="IWO35_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315134.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            41723..41926
                     /locus_tag="IWO35_000043"
     CDS             41723..41926
                     /locus_tag="IWO35_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315135.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(42226..42462)
                     /locus_tag="IWO35_000044"
     CDS             complement(42226..42462)
                     /locus_tag="IWO35_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0315136.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(42645..43016)
                     /locus_tag="IWO35_000045"
     CDS             complement(42645..43016)
                     /locus_tag="IWO35_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315137.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(43013..44341)
                     /locus_tag="IWO35_000046"
     CDS             complement(43013..44341)
                     /locus_tag="IWO35_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0315138.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(44302..44631)
                     /locus_tag="IWO35_000047"
     CDS             complement(44302..44631)
                     /locus_tag="IWO35_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ0315139.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(44889..45260)
                     /locus_tag="IWO35_000048"
     CDS             complement(44889..45260)
                     /locus_tag="IWO35_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0315140.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            45405..45590
                     /locus_tag="IWO35_000049"
     CDS             45405..45590
                     /locus_tag="IWO35_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0315141.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            46533..47192
                     /locus_tag="IWO35_000050"
     CDS             46533..47192
                     /locus_tag="IWO35_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAQ0315142.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            47195..48538
                     /gene="vanS"
                     /locus_tag="IWO35_000051"
     CDS             47195..48538
                     /gene="vanS"
                     /locus_tag="IWO35_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0315143.1"
                     /translation="MERKGIFIKVFSYTIIVLLLVVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQNKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            48709..49515
                     /gene="vanY"
                     /locus_tag="IWO35_000052"
     CDS             48709..49515
                     /gene="vanY"
                     /locus_tag="IWO35_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ0315144.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLRWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            49533..50360
                     /gene="vanW"
                     /locus_tag="IWO35_000053"
     CDS             49533..50360
                     /gene="vanW"
                     /locus_tag="IWO35_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAQ0315145.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            50357..51328
                     /locus_tag="IWO35_000054"
     CDS             50357..51328
                     /locus_tag="IWO35_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAQ0315146.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            51321..52349
                     /gene="vanB"
                     /locus_tag="IWO35_000055"
     CDS             51321..52349
                     /gene="vanB"
                     /locus_tag="IWO35_000055"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAQ0315147.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            52355..52963
                     /locus_tag="IWO35_000056"
     CDS             52355..52963
                     /locus_tag="IWO35_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAQ0315148.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            53550..53981
                     /locus_tag="IWO35_000057"
     CDS             53550..53981
                     /locus_tag="IWO35_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0315149.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            53988..54221
                     /locus_tag="IWO35_000058"
     CDS             53988..54221
                     /locus_tag="IWO35_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0315150.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(54143..54385)
                     /locus_tag="IWO35_000059"
     CDS             complement(54143..54385)
                     /locus_tag="IWO35_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315151.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            54642..54845
                     /locus_tag="IWO35_000060"
     CDS             54642..54845
                     /locus_tag="IWO35_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAQ0315152.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            54929..56122
                     /locus_tag="IWO35_000061"
     CDS             54929..56122
                     /locus_tag="IWO35_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0315153.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            56300..56530
                     /locus_tag="IWO35_000062"
                     /pseudo
     CDS             56300..56530
                     /locus_tag="IWO35_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
     gene            56658..57341
                     /locus_tag="IWO35_000063"
     CDS             56658..57341
                     /locus_tag="IWO35_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ0315154.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            57650..57723
                     /locus_tag="IWO35_000064"
     tRNA            57650..57723
                     /locus_tag="IWO35_000064"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:57684..57686,aa:Arg,seq:tct)
     gene            57882..58241
                     /locus_tag="IWO35_000065"
     CDS             57882..58241
                     /locus_tag="IWO35_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ0315155.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            58364..59995
                     /locus_tag="IWO35_000066"
     CDS             58364..59995
                     /locus_tag="IWO35_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ0315156.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(60057..60698)
                     /locus_tag="IWO35_000067"
     CDS             complement(60057..60698)
                     /locus_tag="IWO35_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0315157.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            60980..61288
                     /gene="rplU"
                     /locus_tag="IWO35_000068"
     CDS             60980..61288
                     /gene="rplU"
                     /locus_tag="IWO35_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ0315158.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            61315..61659
                     /locus_tag="IWO35_000069"
     CDS             61315..61659
                     /locus_tag="IWO35_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ0315159.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            61672..61965
                     /gene="rpmA"
                     /locus_tag="IWO35_000070"
     CDS             61672..61965
                     /gene="rpmA"
                     /locus_tag="IWO35_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ0315160.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            62372..65206
                     /locus_tag="IWO35_000071"
     CDS             62372..65206
                     /locus_tag="IWO35_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0315161.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            65397..65888
                     /locus_tag="IWO35_000072"
     CDS             65397..65888
                     /locus_tag="IWO35_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ0315162.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            65991..66980
                     /gene="manX"
                     /locus_tag="IWO35_000073"
     CDS             65991..66980
                     /gene="manX"
                     /locus_tag="IWO35_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ0315163.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            67013..67816
                     /locus_tag="IWO35_000074"
     CDS             67013..67816
                     /locus_tag="IWO35_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ0315164.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            67837..68751
                     /locus_tag="IWO35_000075"
     CDS             67837..68751
                     /locus_tag="IWO35_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ0315165.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFSPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            68834..69208
                     /locus_tag="IWO35_000076"
     CDS             68834..69208
                     /locus_tag="IWO35_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ0315166.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            69347..69592
                     /locus_tag="IWO35_000077"
     CDS             69347..69592
                     /locus_tag="IWO35_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0315167.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            69764..70828
                     /locus_tag="IWO35_000078"
     CDS             69764..70828
                     /locus_tag="IWO35_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0315168.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            71074..71499
                     /locus_tag="IWO35_000079"
     CDS             71074..71499
                     /locus_tag="IWO35_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0315169.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            71492..71941
                     /gene="nusB"
                     /locus_tag="IWO35_000080"
     CDS             71492..71941
                     /gene="nusB"
                     /locus_tag="IWO35_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ0315170.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            72038..72892
                     /locus_tag="IWO35_000081"
     CDS             72038..72892
                     /locus_tag="IWO35_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ0315171.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            72893..74230
                     /locus_tag="IWO35_000082"
     CDS             72893..74230
                     /locus_tag="IWO35_000082"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ0315172.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            74235..74459
                     /locus_tag="IWO35_000083"
     CDS             74235..74459
                     /locus_tag="IWO35_000083"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ0315173.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            74461..75351
                     /locus_tag="IWO35_000084"
     CDS             74461..75351
                     /locus_tag="IWO35_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0315174.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            75360..76178
                     /locus_tag="IWO35_000085"
     CDS             75360..76178
                     /locus_tag="IWO35_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ0315175.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            76285..76734
                     /locus_tag="IWO35_000086"
     CDS             76285..76734
                     /locus_tag="IWO35_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0315176.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            76752..78434
                     /gene="recN"
                     /locus_tag="IWO35_000087"
     CDS             76752..78434
                     /gene="recN"
                     /locus_tag="IWO35_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ0315177.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(78489..78605)
                     /locus_tag="IWO35_000088"
     CDS             complement(78489..78605)
                     /locus_tag="IWO35_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ0315178.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(78678..79634)
                     /locus_tag="IWO35_000089"
     CDS             complement(78678..79634)
                     /locus_tag="IWO35_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0315179.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            79805..80173
                     /locus_tag="IWO35_000090"
     CDS             79805..80173
                     /locus_tag="IWO35_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ0315180.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            80343..80774
                     /gene="mraZ"
                     /locus_tag="IWO35_000091"
     CDS             80343..80774
                     /gene="mraZ"
                     /locus_tag="IWO35_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ0315181.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            80795..81754
                     /gene="rsmH"
                     /locus_tag="IWO35_000092"
     CDS             80795..81754
                     /gene="rsmH"
                     /locus_tag="IWO35_000092"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ0315182.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            81759..82160
                     /gene="ftsL"
                     /locus_tag="IWO35_000093"
     CDS             81759..82160
                     /gene="ftsL"
                     /locus_tag="IWO35_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ0315183.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            82161..84353
                     /locus_tag="IWO35_000094"
     CDS             82161..84353
                     /locus_tag="IWO35_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0315184.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            84379..85341
                     /locus_tag="IWO35_000095"
     CDS             84379..85341
                     /locus_tag="IWO35_000095"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ0315185.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            85355..86716
                     /locus_tag="IWO35_000096"
     CDS             85355..86716
                     /locus_tag="IWO35_000096"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ0315186.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            86742..87830
                     /gene="murG"
                     /locus_tag="IWO35_000097"
     CDS             86742..87830
                     /gene="murG"
                     /locus_tag="IWO35_000097"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ0315187.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            87845..89065
                     /locus_tag="IWO35_000098"
     CDS             87845..89065
                     /locus_tag="IWO35_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ0315188.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            89221..90546
                     /gene="ftsA"
                     /locus_tag="IWO35_000099"
     CDS             89221..90546
                     /gene="ftsA"
                     /locus_tag="IWO35_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ0315189.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            90569..91810
                     /gene="ftsZ"
                     /locus_tag="IWO35_000100"
     CDS             90569..91810
                     /gene="ftsZ"
                     /locus_tag="IWO35_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ0315190.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            91827..92504
                     /locus_tag="IWO35_000101"
     CDS             91827..92504
                     /locus_tag="IWO35_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ0315191.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            92518..93117
                     /locus_tag="IWO35_000102"
     CDS             92518..93117
                     /locus_tag="IWO35_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ0315192.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            93132..93419
                     /locus_tag="IWO35_000103"
     CDS             93132..93419
                     /locus_tag="IWO35_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ0315193.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            93468..94250
                     /locus_tag="IWO35_000104"
     CDS             93468..94250
                     /locus_tag="IWO35_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ0315194.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            94271..94978
                     /locus_tag="IWO35_000105"
     CDS             94271..94978
                     /locus_tag="IWO35_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ0315195.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            95240..98038
                     /gene="ileS"
                     /locus_tag="IWO35_000106"
     CDS             95240..98038
                     /gene="ileS"
                     /locus_tag="IWO35_000106"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ0315196.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(98087..99613)
                     /locus_tag="IWO35_000107"
     CDS             complement(98087..99613)
                     /locus_tag="IWO35_000107"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ0315197.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(99628..100275)
                     /locus_tag="IWO35_000108"
     CDS             complement(99628..100275)
                     /locus_tag="IWO35_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ0315198.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(100459..100788)
                     /locus_tag="IWO35_000109"
     CDS             complement(100459..100788)
                     /locus_tag="IWO35_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ0315199.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            100965..101693
                     /gene="sfsA"
                     /locus_tag="IWO35_000110"
     CDS             100965..101693
                     /gene="sfsA"
                     /locus_tag="IWO35_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ0315200.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            101709..102722
                     /locus_tag="IWO35_000111"
     CDS             101709..102722
                     /locus_tag="IWO35_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0315201.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            102722..103999
                     /locus_tag="IWO35_000112"
     CDS             102722..103999
                     /locus_tag="IWO35_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0315202.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            104062..106764
                     /locus_tag="IWO35_000113"
     CDS             104062..106764
                     /locus_tag="IWO35_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0315203.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            106916..107233
                     /locus_tag="IWO35_000114"
     CDS             106916..107233
                     /locus_tag="IWO35_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0315204.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            107263..107583
                     /locus_tag="IWO35_000115"
     CDS             107263..107583
                     /locus_tag="IWO35_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0315205.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            107691..109151
                     /locus_tag="IWO35_000116"
     CDS             107691..109151
                     /locus_tag="IWO35_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0315206.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            109219..109440
                     /locus_tag="IWO35_000117"
     CDS             109219..109440
                     /locus_tag="IWO35_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315207.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            109471..109653
                     /locus_tag="IWO35_000118"
     CDS             109471..109653
                     /locus_tag="IWO35_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ0315208.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            109653..110066
                     /locus_tag="IWO35_000119"
     CDS             109653..110066
                     /locus_tag="IWO35_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ0315209.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            110189..111370
                     /locus_tag="IWO35_000120"
     CDS             110189..111370
                     /locus_tag="IWO35_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0315210.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            111759..111832
                     /locus_tag="IWO35_000121"
     tRNA            111759..111832
                     /locus_tag="IWO35_000121"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111793..111795,aa:Arg,seq:cct)
     gene            complement(111869..112069)
                     /locus_tag="IWO35_000122"
     CDS             complement(111869..112069)
                     /locus_tag="IWO35_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0315211.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(112374..113606)
                     /locus_tag="IWO35_000123"
     CDS             complement(112374..113606)
                     /locus_tag="IWO35_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0315212.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            113860..114429
                     /locus_tag="IWO35_000124"
     CDS             113860..114429
                     /locus_tag="IWO35_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0315213.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLC
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(114607..115047)
                     /locus_tag="IWO35_000125"
     CDS             complement(114607..115047)
                     /locus_tag="IWO35_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ0315214.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            115205..115969
                     /gene="map"
                     /locus_tag="IWO35_000126"
     CDS             115205..115969
                     /gene="map"
                     /locus_tag="IWO35_000126"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ0315215.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            116001..116924
                     /locus_tag="IWO35_000127"
     CDS             116001..116924
                     /locus_tag="IWO35_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ0315216.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            117000..118139
                     /locus_tag="IWO35_000128"
     CDS             117000..118139
                     /locus_tag="IWO35_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ0315217.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            118132..118932
                     /locus_tag="IWO35_000129"
     CDS             118132..118932
                     /locus_tag="IWO35_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0315218.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            118932..119759
                     /locus_tag="IWO35_000130"
     CDS             118932..119759
                     /locus_tag="IWO35_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0315219.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            119737..120471
                     /locus_tag="IWO35_000131"
     CDS             119737..120471
                     /locus_tag="IWO35_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0315220.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            120571..121437
                     /gene="rfbA"
                     /locus_tag="IWO35_000132"
     CDS             120571..121437
                     /gene="rfbA"
                     /locus_tag="IWO35_000132"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ0315221.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            121451..122023
                     /gene="rfbC"
                     /locus_tag="IWO35_000133"
     CDS             121451..122023
                     /gene="rfbC"
                     /locus_tag="IWO35_000133"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ0315222.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            122045..123073
                     /gene="rfbB"
                     /locus_tag="IWO35_000134"
     CDS             122045..123073
                     /gene="rfbB"
                     /locus_tag="IWO35_000134"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ0315223.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            123171..124022
                     /gene="rfbD"
                     /locus_tag="IWO35_000135"
     CDS             123171..124022
                     /gene="rfbD"
                     /locus_tag="IWO35_000135"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ0315224.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            124057..126090
                     /locus_tag="IWO35_000136"
     CDS             124057..126090
                     /locus_tag="IWO35_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315225.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            126134..127414
                     /locus_tag="IWO35_000137"
     CDS             126134..127414
                     /locus_tag="IWO35_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ0315226.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            127624..128430
                     /locus_tag="IWO35_000138"
     CDS             127624..128430
                     /locus_tag="IWO35_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0315227.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            128442..129662
                     /locus_tag="IWO35_000139"
     CDS             128442..129662
                     /locus_tag="IWO35_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0315228.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            129652..131238
                     /locus_tag="IWO35_000140"
     CDS             129652..131238
                     /locus_tag="IWO35_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0315229.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            131277..133415
                     /locus_tag="IWO35_000141"
     CDS             131277..133415
                     /locus_tag="IWO35_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0315230.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAESTHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(133783..134766)
                     /locus_tag="IWO35_000142"
     CDS             complement(133783..134766)
                     /locus_tag="IWO35_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAQ0315231.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            135144..136535
                     /locus_tag="IWO35_000143"
     CDS             135144..136535
                     /locus_tag="IWO35_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0315232.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            136551..137591
                     /locus_tag="IWO35_000144"
     CDS             136551..137591
                     /locus_tag="IWO35_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0315233.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            137610..>137801
                     /locus_tag="IWO35_000145"
     CDS             137610..>137801
                     /locus_tag="IWO35_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAQ0315234.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPN"
BASE COUNT        42684 a        26350 c        32045 g        36722 t
ORIGIN      
        1 attcttctcg tcgtcttggt tccccgaatt tttggtttga ctccttatat tattgaggac
       61 aaagaaatga gtccatatta tgcacaagga aatctgatat atgtacgacc gaaaaataca
      121 aatgagattt tagtcggaga tattattaca tattatgaaa attcaggaaa aaaaatctcg
      181 acgcgtcgcg taatagccgt tgaaaacaac cacgaagatt tttcctcaaa attttaggtt
      241 atcgctgaaa gccccggaaa tacgggcatt tcgcaggata tgagaactca atttactacc
      301 aatttactac tttcggattg agccgcatat atgctgaacc atactgctaa tacccgccac
      361 cccaaatacg gcacatcata aattgaatac gacaccgtaa acgcggcgtt cctctttccc
      421 gtcatgggag aacaggacgc cgctttttta tgcccaaata gagaaaggag gcgacgccat
      481 gctatttact tccggccctg accgggcgtt tgaacggctg atgcaggaaa agcccggttt
      541 cgatcactac gacggcggcg gggcggacgc gccggaggat tgcggcacct gccgatttta
      601 ccgcccggat tggaaatatc aattctgcca gtttgcagag tgtccctatc agccgggcaa
      661 gctgaccgac tatgcagcgg tcaaattcac agtgaaagac ctgctaataa aagtcaagta
      721 gaaatttcag atttttaggg aggcgaaaaa atgcaataca aaatcaagcc gctgagcatc
      781 tcaaaattaa aacggcggcc agccagatgg tatagagtgt aaaaatcagt cgtgctccag
      841 ggattccaag taggctttca cagaagcgta gtagatgcgc aggaacttgt tggcagaggc
      901 catcatgtag acacgatatg gcttgccttc agagcgtttc ttgtccatga actggtacac
      961 cggctcgtct attggagcac attgcaggat gacgctcatc accagaaaga gcgtcctgcg
     1021 cagtgaggca gatcctcgct tggaaatgct acggctacgg acatctattt gaccggattg
     1081 gtagggcggg gcgtcaatac ccgcaaaggc aaccaacgct tttttggaat gaaaacggcg
     1141 tacatcgcca atttcagcca ggagttgagg gccgagggtt gggccaacac caaacatccc
     1201 catcactaca ggatactccg gcagagaagc tgccagggat tgcatctcct gtttgagagc
     1261 agctaacgcg gaagaagttg tctggagttg agaaatggcc tgttctacca aaagttttgc
     1321 cgtatccgtt ttcggtatga caccgaagtg tctgcaagca gaggcataaa tatccagcgc
     1381 cttatcctcg ctgaaattgt agccgtgctt tctgcaccac ttctggtatt gggtagtaaa
     1441 ggccttctca gaccggccac aaacgcactc gcaatgccaa aaagtagcga caaagtccac
     1501 ccacttctcg ctgccatcgg cgcggggcgg actggtaaac aagcgatttg cgtctgggaa
     1561 ggtggtgtcc agcagggaga tcaggttatt cttcagcatg gtctgaactt tggaatactg
     1621 ttggtactgc cggtagcaga ttttcagcat gagtcgggta tcctcctccg ggatatatct
     1681 cggcaatgtg agccagtggt caagaccgta gttggccagc tttacggcat cctttttgtc
     1741 ggttttggcc cgtcttaaac tgttgtttcc gtagtcatgc accagcattg cattgactac
     1801 cgagacataa aggcccgcgt cgtggagcag ccaggccacc ggagcatggt aattacccgt
     1861 ggattccatc accacacggg tctcgccgtc caggcttttg agcagtcctg ccagctcgct
     1921 cagttcattg gcggtgtgac gtacttcaaa gggtgaaacc actacctctc cgaagggccg
     1981 catgactgca atcatgctct tacccttgga aacatcgatg ccaacgcagt tcattcacat
     2041 tcctccaata caaagaatct gcaaccggaa tccatctttt ctcgctgccg attcaatcta
     2101 ttgtgtgaca cgaactctcc gacatccggt ggttcaacct gcttaaatcg aacgctgcaa
     2161 cgagaggatg gctaacagtc tttcacacgg acatacaagc ccaaggagtg attggtcagc
     2221 cagttgctcc tctcattgta gcttaggcac gagatggagg gaaagccgga ctggctgtcc
     2281 ggctttcctg tccaaattta ttgtaatagg agcgaacaac gctatggcag ttttccgtgt
     2341 ggaaaagaac aaaggctata cggtgatgtc aaatcaccac ctgcggaata agaacctgac
     2401 cttaaaggcc aagggcctgc tgtcgcagat gttgtccttg ccggaggatt gggactatac
     2461 cctaaaagga ctgtcctata tcaaccggga aagcatcgac gcgatcagga cagcggtatg
     2521 ggagcttgaa aaagctggat atatccggcg tgagcagggc cgtgacgcaa aaggcaaaat
     2581 ggccgatatg gtctatacca tctacgaaca gccggtattg gaaaacccgg tattggaaaa
     2641 tccaacatcg gataacccgg tgttggaaaa tccgacatcg gataatccgg cgtcggaaaa
     2701 tccaacgcaa ttaaataaag aagtacaaag aactgactta tcaaaaaaag aaaaaataat
     2761 tacagatgga caaagtaccc attccattcc tatcccatcc ccgacccctg cccctatgga
     2821 gggcgaggca gcggctacgc cgccggaacg gaaaaggaca ggaaaggatg aggctgtacg
     2881 gatttatcgg gaaatcattt tggagaacat cgagtatgcc tatctcattc aggacaggag
     2941 cattgaccgt gaacagcttg acgaaatcgt agacctgatg ctggaaaccg tctgcaccgc
     3001 ccgaaagaca atccgtgtcg ccggggacga ctaccctgcc gagctggtga aatccaagta
     3061 catgaagctg accggcgaac atatccgctt tgtccttgac tgtctgcggg agaacacaac
     3121 gaaagtccgc aatatcaaac aatacctgcg ggctatgctg ttcaacgccc ccagcaccat
     3181 ttccaactac tacacggccc ttgtcgcgca cgacatggca cagcccgatt ggggaaagcc
     3241 taaatccggc ctgccggact attcctgcgg cgagggcgaa agcctgtaac gagaaaagga
     3301 ggacgaccct atgaagcagg gcgcattgat ttttgacgag cgaacagacc gttacgacat
     3361 ccgctttgat ctggccgact attacggcgg cctgcattgc ggggagtgtt tcgacgtgat
     3421 gatcggcggc aaatggaggc ccacccgcat tgaaatggcc gaaaactggt atcttgtcgg
     3481 catccgggcc gacgaccttt ccggcctgcg ggtgcggatt tgacctatat ctacctgcgg
     3541 cgtctggtgg ttccgcctta gacgccgctt ttgactttct gacaaggagg gatgcgattg
     3601 caggaagaag ttactcaaaa gaccattgcc ctgtccatga aaacgggcaa gctcacggct
     3661 caagtgctgc aagcggcgct gaaaaagtac ctgcaacacc gggccaaggg caaaaacacg
     3721 ctgcaccacg gccagcagag cctaaagcag cttaaaaagc atggggctga tctctccaac
     3781 attgagatca ccgaggccaa cattggagcg ttcaagccat gcgccaagaa atacggcgtg
     3841 gattttacct tacgaaaaga taagaccacc cagccgcccc actacgttgt gattttcaag
     3901 gccaaggatg cagataacct tgatctggcg tttcgggagt ttaccgccaa gacgctttcc
     3961 aaggagcagc ggccctctat ccgcaaggtt ttgtccgctg tgaagcaaaa ggcggcggcc
     4021 cagaccaagc agcgggccaa ggagaaaatc aaggaaaggg ggctggaact gtgaagcctg
     4081 acgtaaagaa attgctgatc ctgaacctcc cgtatctgat ttttgtgtac ctgtttgcga
     4141 aatgcgggga ggcgtaccgg ctggccgctg gtgctgacct gtcggggaag ctcctgcatt
     4201 ttgcggatgg ttttgcggcg gcttttgcca acccgctgcc aagcctgcac ctgtttgacc
     4261 tgtgtatcgg tatcgccggt gccgtgatcg tgcggctggt ggtgtactgc aagagcaaga
     4321 acgtgaagaa ataccgcaaa ggcatggagt atggcagcgc ccgctggggg acggccaaag
     4381 acatagcgcc ttacatcgac cccaagtttg aaaataatat cctgctcacc caaacggaac
     4441 ggcttaccat gaccggcagg cccaaagacc ccaagacagc gcggaataaa aacgtgctgg
     4501 tgatcggtgg cagcggttca gggaaaacga gatttttcgt gaaacccagt gcgcccgtaa
     4561 gggcggtata aatcctacct tgagcaagta gtaggtaaaa gtatcaagcg gcattgtgcc
     4621 gcaacctatc attccccgtc ttaccccaaa agggaaaccg gagggcgacc atagcatgac
     4681 ggaggacacg gggcgctgaa gcctcagaag cgccggcgtg acggaatgaa aatccacgta
     4741 tgaggatgga agctagactg cttgaatgac agcctgaaat cgggaccaag agcgtacccc
     4801 tgacgtagag aggttgtact cgtcagtgtt cctgacagtc gcattgtttg gctatgcggc
     4861 tgctgatact cggagcaggt tcagccacgg gaaagtggaa aaaggcgaac gtcacatacc
     4921 gacaacgtgg aacgcttgtt tataccgtac taaacgggga ttgcctaaag tgaaatgccg
     4981 aaaggctatg aaaacgctga aatgcggggt tctgaatatt caccatggca acagagttcc
     5041 catagtagtc tgcggacggg aaagccgtct acatggcaaa ggggaacagt gaatcatttt
     5101 caaaacagaa aggatggtgt gtgagacact atgagaaatc cgattcatgt cttgaaaagc
     5161 cttgaagaaa aggcaagtgt aagtaactac aaatatgaaa gattatatcg caacctatac
     5221 aatccagagt tctatctcct tgcatatgcg aacattgcga aatcgcaggg gagcatgacg
     5281 cagggggtag acgggcagac gctggacaac atgagcctgc cgagaattaa ccggattatt
     5341 gaatccatac gcaacaggac atatcagcca aagcctgcaa aaagaaagta cattcctaaa
     5401 aagaacggga aacttcgccc gttgggaatc acttctactg atgataagtt agtgcaggaa
     5461 gtggtcagaa tgattcttga agcaatctat gagccgacat tcagcaacaa ttcacacggt
     5521 ttcagaccaa aaagaagctg tcacacggca cttacgcaag tgaagaaaaa cttcacaggt
     5581 gttacatgga ttgtcgaggg agacattaag gcgtgctttg ataacttcga ccatcatgtg
     5641 ttggttgaat tactgcggaa aaggatttca gacgaggctt ttatcggtct aatctggaag
     5701 ttcctgaaag ccggatatat ggaacaatgg cagtacaact gcacctactc cggtgttccg
     5761 cagggcagcg gtatcagtcc gatatgtgca aacatctacc tcagcgaact ggacaactat
     5821 atgcaggaat acaaagaaaa gtatgattgc gagccagaac gcagaaggac gaccagagaa
     5881 tacgagcggg cgtcccggag atacagaaag gcacgtaaag cgttaatggg cgcagaaaaa
     5941 tcaactcctg aactggtaaa agaatttaag gattccagaa ggaagaagat gaatcagcac
     6001 tattacaatc cgtttgagga aggcttcaag aaaatccagt acaaccgtta cgccgatgat
     6061 tttgtaatcg gcgttatcgg ctccaaaaag gacgcagaaa aaatcaagga agatgtaaaa
     6121 atctttctcc aagaaaaact gcatttggaa atgtccgagg aaaagacgaa agtcacccat
     6181 tccagtaagc cggtacgcta tctggggtac gatttcaaag taatccattc caagaacatg
     6241 aagcgttgta aaaacggcga catgaaacgg gtgtggtatg gaaaagtatt cctgtatatg
     6301 ccgaaagaaa aatggattaa aaaagcgatg gaacggggag cgatacaggt gaaacgcaac
     6361 aatgacacag gcaaagaaat gtggcggcct atgccaagga aagacctgat gaaccgcagc
     6421 gacgcagaga ttgtgtctac tttcaattcc gaaattcgag ggttatataa cttctatcgg
     6481 atagcagaaa acgtaggcgc attgcacaag tattactaca tggtgcggta cagcatgtta
     6541 aaaactctgg caggaaagca cagaacgaat gtcagtgtta ttaagaagcg gcacatggta
     6601 aacggagtac tgagaatccc atacgataca actaagggac gtaaatactg cgaattttac
     6661 catgatggat tcagaaagca cagcgacggc tatgacaatg tggcagacgt tatgccgagt
     6721 tataggaaat atgacagcag gcacacgata gtaaaccgca taaaagccgg agtatgcgag
     6781 atttgcgggg aacatgcaga ctatttatgt atgcaccacg taagaacgct gaaatcgctg
     6841 aaaggcaggg atatatttga gcagaaaatg ctgaaaatga gaagaaaatc tctggctctc
     6901 tgccctgact gctttgaact cttacacgaa accaaagaat caaggtgatt catggaaagc
     6961 cggatacgct gagaggtgta cgtccggttt gggaggcggc tccctgaaac ctactgatga
     7021 aagtcagcaa ggcgcagggt gcctacctca tacctgatgc aatgctttcc gaccacggac
     7081 tatccgactt cgtttgtggt gacagatccg aaaggcactt tggttctgga aaccggcagg
     7141 atgttccagc aggcgggcta ccgcattaaa atcctgaata ccattaactt ttccaagtcc
     7201 atgaagtaca acccctttgt ctacatccat tcggaaaagg acgtgctaaa gctggtaaat
     7261 accctgattt ccaacaccaa gggcgagggg gaaaagtcag cggaggattt ttgggtgaag
     7321 tcggaacggt tgttttattc ggccctgatc ggctatatct ggtacgaggc cccagcggag
     7381 gaaatgaact tctcaacgct gctggaaatg ataaacgcca gtgaagcccg cgaggacgac
     7441 ccggagtttc aaagccccgt cgatctcatg tttgagcgtt tggagcagaa agaccccgac
     7501 cattttgcgg tgcgccagta caagaaattc ctgctgtccg caggcaagac ccgttcttcc
     7561 atcctgattt catgcggtgc cagacttgcg ccctttgaca tccgggaggt acgcgagctg
     7621 atggaggacg acgaaatgga gctggacacc atcggggacg aaaagacggt gctgtatctc
     7681 attatgagcg atacggacac gacctttaat ttcattctgg ctatgctcca aagccagcta
     7741 atcaacctgc tttgtgaccg ggctgatgat gtgtacggcg ggcggctgcc tgtccatgta
     7801 cgcctgattt tggatgaatt tgccaacatt ggccaaatac ccaatttcga taaactcatt
     7861 gccaccattc gaagccgtga aatatcggcc tctattattt tgcagagcca gtcgcagcta
     7921 aaagcgattt acaaggacgc cgccgagatc atttcggata actgcgatag tgtgcttttc
     7981 cttagtgggc gcggcaaaaa tgccaaggag atttccgatg cgctggggaa agaaacgatt
     8041 gacagtttca acaccagcga gaaccgggga agccaaacgt cccacggatt gaactatcaa
     8101 aaattaggaa aggagttgat gtcccaagac gaaattgccg tgatggacgg cggcaagtgt
     8161 atcttgcagc tacggggtgt gcgcccgttt ttatcggaga aattcgatat taccaagcat
     8221 ccccggtaca aataccttgc cgacgccgac aagaagaata cctttgacgt ggacagattt
     8281 ttgactatca cgcgccggaa acggcagcaa gtggtaacgc aggatgaagt attcgacctg
     8341 tatgaaatcg acctgtcgga cgaggacgcc gccgctgaat aaacagcggc ttttttcatt
     8401 ccaacaacaa aatcaaaaaa tcattatgag ccggaaaccg gcagaaagag gtaaatttat
     8461 ggcatttttt aattccgcag ttactacttt gcagactatc gtgatcggac tgggcggcgc
     8521 tctgtgcgta tggggcggca tcaatctgtt ggagggatac gggcaggaca atccgggggc
     8581 aaaatcgcag ggtgtcaagc acttaatgtc ttctatgtga aaagatttta aagagaaaat
     8641 gcacatccta cgcagataac atgacaattt catgcaaagc gaaaggagac accatgaaaa
     8701 tcgaatacac aaaagtggga gactattatt taccgaatct ttactacccg gaggaaacca
     8761 gaccgattgg cttatgggga atgcttcgga aggaatatct gaaagaacat aaatctggaa
     8821 cttatacata tcttcttctg accgccaggc tggacagtta tttggcagat ctgaatgaac
     8881 aggcacagga acgctttgaa ttgatcgaaa ctcagatgcg aagtgcggag ggagtttccg
     8941 aggaactgaa gagacagaat cctatggaat ggctatgcca ctgtaacaat atccgcaacc
     9001 gggctgctga gatcatcaag caggaactga tttatgtata agggggattc tagatatgag
     9061 aagagaactg attgaaagtt attatgaaga ataccagcgg gagctggaaa gatttcttga
     9121 atctgacccg gagatgttca ctctggaagc agaaattcat cattacatag aaatactgga
     9181 accggtcctc agagatggcg gacaggagat gtggcagaca ctggacagga ttatttcagc
     9241 gaaaaatgca atggagggaa ttgtggcaaa agaaatgtat atccggggat ttctggatta
     9301 tgaaagactg gtgtgtgaaa aagacagtaa gatctgaata atcagaacat agactatata
     9361 taacgaggct tttattgtat agacagtaag agcctcattt aatatagttt tcataataaa
     9421 aaacaggtgg tagtacgtat ttgctttgtg ttatataaga attggtctgg ctgaacaatg
     9481 gtgaaaattt actatgattt tacttaaatc tgattgaagg atgaaaaagt gatgatatca
     9541 tatagtgggt tgatagggtt acagaaaatg tattcacaga ggcagagaag aaataatgaa
     9601 agtaaaaaat gcagatgaat tttataaaat attagaaaga aaggctatat atccggtatt
     9661 tcagccgatt gtaaatttac agactggaga tgtggcagga tatgaagctc ttagtcgtat
     9721 cgacaggcat gatacgactc ttatgatttc agatttgttt gtgattgctg aacaggtagg
     9781 ctgtgtttgg aaattggaaa aattgtgcag aaataaagca ttgaaggcag cggcgaataa
     9841 accagaacat gcaaaattat ttcttaatgt agatggtaat atcattcagg ataaatcgtt
     9901 catacaggga tttacaaacc gtaaggctgc gaaagccgga gtaccgtcct gtgacattgt
     9961 atttgaaata acggaacggt cggatataga aaactatcag attctgcaac agattatgaa
    10021 gcattatgca gatcagggat atgaaatagc tttggatgat gttggagctg gatattctgg
    10081 tctgaaccgt gtagtaaata catcgccaaa ctacttaaaa gttgatatag aactggtcag
    10141 agatattcaa aaacataaga aaaaggaaat tatgatggaa tttctgttgc attactgtaa
    10201 tgagacagga gcaattctga ttgcagaagg gatagaaaca gaagcagagt tggagtgtct
    10261 gtgtagatta ggtgttcatt acggacaagg atattttctt ggaagaccgg acagaacgtt
    10321 caaaaatgta aggaatgagg cgatgttggt attacgtaga ctgaataata aatcgtaatg
    10381 aaaactatat gtcagcaggt gaaaaatgaa taagtcaatt ataacattat aaagatttcc
    10441 attgattttt acacctattt tacacaagca ctcacataga tttgatttct gtcttctata
    10501 attgtatagg aaaatgtaga tcttatataa taacaggaga ggaaagagaa atgaacgaga
    10561 atgtgaaagt ggtttttggt ctgatcggtg gccttgcctt gttcctttat ggaatgaaca
    10621 gcatgagtga tgcacttcag aaagccgcag gagaacgaat gaaaaaaatc cttggatttc
    10681 ttacaagaaa tccaattatg ggagcacttg ccggagcact tgtaacagct gtattgcaga
    10741 gcagttcagc gacgacagtt atggttatcg gttttgtcag tgcgggactt atgagtcttc
    10801 cgcaggcaat ttctgttatc tttggtgcca acataggaac cactatgacg gcacagctga
    10861 tggcatttaa aatcagcaat tatatttatc caattatctt tgttggattt attctgaact
    10921 ttgttagcaa aaaagaaaag gttaaaaata tcggtatggt aattttttca ttcggacttt
    10981 tatttgaagg aattgaaatc atgggtgaag tcatgaaacc tttggcggga agtccggtct
    11041 ttgttgatct gatgggaaaa gtttcatcca ttccggttct tggtgttgta ctgggagcgg
    11101 tcatgacatt ggttgtgcag agtagttcag ctacgattgc agttctgcag aattttgcat
    11161 cacaggcagg accggacgga gtaagcagtg taatagggct taccggtgcg attccgattc
    11221 ttcttggaga taatataggt acaacgatca cagcacttct tgcatcaatt ggacaatcta
    11281 aaaatgcaaa acgtacagca attgcgcata gtatttttaa tatcagtgga agctgcgttt
    11341 ttattttcct ggttccgtgg tttgcaaaat ttgtccagtt tatttcacca aagggaaatg
    11401 agattgatgt gatttcaaga cagattgcaa atgcacatac aacgtttaat atcgtctgta
    11461 cactggtctg gctgccactg attccgctga tggtaaaaat tgttacaaca atcatccgtg
    11521 gaaatgacaa aacagagaaa gcagcttttg aacccaaata tctggatatg aaagtgattg
    11581 aacagccggc ggctgctatg gttctggtat ctaaggaact gaaccgactt ggagaactgg
    11641 cagaatctct gttaagtgat ttaaaaacgg cgattgtagc agatggagac tcaaaaactc
    11701 atggttcgtt tatagaaaat ctggagattg tacatcagtt acaggacagt gtgacagaat
    11761 atattaccag attattcgca agcggaaacc ttacagaaca acagtctgag cagacggcag
    11821 ggttactgta tgtaaacaat agtattcaga ggattgcaga cagatgtgaa gatattgacc
    11881 agatatgtga aaaagtaaat aacaatggaa aaagactgac cagtgaagcc tcctcagaaa
    11941 tggaagactg cattgatatt atccagcagc ttttaaagaa agcaatggag gcaatccgta
    12001 agggtgatgc tgtagtggct gaaacagttt ttaaaaataa gaaaaaaatg cataaagcag
    12061 agaaaaaata cagtaaggca catttaaacc gtgtaaaaaa tgaggtttgt gatgcgtcta
    12121 tgacaagata tttttctggg atcatgtata attttgaccg tatggcagat aattgtgtca
    12181 gcattgcaga agaagcctgt gataatgtgg cttttataaa ccttgatgaa gagacgggaa
    12241 aatcaatgat ggaaagaggt gcagtataaa atggtaaaga aaaaaaacat ccgaaaatct
    12301 gcacttctgc tgataatgat aatgctggca acgggaataa cagcatgtgg aaccgaggaa
    12361 gagccattgc cggatctgtc tgctatggga gctgtcacag ctgtatcacg agaagaaggg
    12421 tcaggaacca gagcagaatt tgagaatctt ttgaaactgc ctgagagtga tacaggaata
    12481 gttgttgatt ctacagagaa agttctgaaa aaggtggaag aagataaaaa cgcagttggt
    12541 tatgtcgcat atagttccgc aacagatacg aatggaaaaa tacttcaaat caacggagtt
    12601 cttccttctg agaaaacaat agacaataac agttatcctc tttgcaggga ttattatctg
    12661 gcttataatg gagaactgac agatgtggaa caggatttcc tgacttatgt aaagagtaaa
    12721 ggacaggata ttgtgaaaca atattgtatt caggctgaca gtacaacaac attcctttct
    12781 gacaagtcag aaggaaagat tctgattgaa ggatccactt caatggaacc aatggtaaaa
    12841 gcactggcag atgactatca gaaacaaaat ccaaatgctg aaatagaagt aaaagcaaca
    12901 gattcttcaa gagggattac ggcggtgata agtggagagt gtgatttcgc aatgtcatcc
    12961 agagaattaa aggattatga agcagagctt ctggaaacaa aagtcattgg aaaagatgct
    13021 atagctattg tgataaatga agaaaatcca ctggaaaatc ttacaataaa acaacttaca
    13081 ggtctgtaca atggaacata taaaaactgg gatgatcttt cctgagaaaa acgaatataa
    13141 tatcttacaa gcactgacta taacggtcgg tgcttctttt ttggattttt tcaaaattat
    13201 ctgacattct tcaaattccc acgtgtgatt agtggaaaaa taattttttc tgcaaaagta
    13261 ccgtccaaat gccctctgaa acttgctatt agtgagggaa tatttcattt atgcgaaaaa
    13321 aacttgcaaa agggggttgg taaataccgc ttcccaggct ttataggtag aggaacaatt
    13381 attttaaaaa ttttacccca aagatgtccg cttttgcctg tctcagtaca gagtatgtga
    13441 agggactaac aaaagtaaaa gaaaaaatga taaaaatgca aaaaaatatc acgaatcttt
    13501 tgcatcttgc gaagtcattt gactctagta atcagaggac gttttggaat gttccttcga
    13561 tgaaccttga caaccgataa gtgcacccta gaagcctaac aggagttatg ccatgacctt
    13621 tctttttctg agagcgagcg aaattataat gacactcccg atgatgggcg gctgaccgaa
    13681 gccttatggt cagtgacctg ctgatgcagg cagaggcagt ctgagacggc tgcaggtgac
    13741 agggaaacgc acgccagacc gccggacgaa tgaaactgat gacaatgttt acagggcaaa
    13801 atgcccggaa aggagcagtt tatgacagaa ctgaaaatga aagaaatgca gttgattaac
    13861 tggtttggat gcccgaatct gatttataca agggagagaa tccatctggt tgccatgctt
    13921 acaacagatg aagaaatgaa aaagaatctt tacagggtat gcagattcct tgcaagagaa
    13981 gaaatcgcat atcggtatcc gatcatgtat gaaatgatcc ggaaaatatt ttcaccggat
    14041 gataccaatc caccggaagc atgtgcaatg tttatgccgg acgatgaaac agatgatgaa
    14101 gaatatgcaa tggcagcgta gctgcggaaa ggacagtaac gattatgaat aaagtggttt
    14161 taatgggaag actgacaaag aatccggaga tcaaatatgc cggaaaagat aatgatatgg
    14221 cagtagcaag atatacactg gcggtaaaca gaagatataa acgtgacggt gaacaggaag
    14281 cggattttat ttcctgtgtc acattcggaa agagtgcaga gtttgcacag aaatatcttc
    14341 acaagggaat gaggattgtg attggcggtc gtatcagcac tggcaattat aaggacaagg
    14401 acgggaaaac aatttatact acagatgtga tcgtggaaga acatgaattt gcccagaaca
    14461 aggataatgg tgttggagct gattcatcgg aaacaccgaa aacagataag gacggattta
    14521 tggaagcacc ggaaggtgag gttccattcg actaaaccga agtaaatggt tggagaggtt
    14581 gccctttgtc ggggcatcct cttttccatt ttacagaaga catcaaaaat tacaggtatt
    14641 caacctaacg aaatcgtgat ataatcaaaa tatgttcaga tgaaaaatat ggaggttgct
    14701 tatgggcgta agttataaca gattatggaa aatgctcata gataaaaata tgaagcggat
    14761 agagatgcag tacctcaccg ggatcagtgg aaatattctt gccaggatgg gaaaaaacca
    14821 gtatgtatca atggagacga ttgagaagat ctgcaagaag ctggactgca ccgttgatga
    14881 gatgctggaa tttacagatg atgagtaaca agagcagaca tgtcaaatta ttaaaactgg
    14941 aagaatgctt cagaagtcga aaaaagaaat tgaaaaaaga taacacgccg gatacggcat
    15001 aaatctctta gcaacgctaa gagcgcactt ctatacaggc aattccatct gcctgtatcg
    15061 tgcgacctgc ggtgctgttc ccgatttcaa gaaatctcaa accgggaaat acagcgaaat
    15121 cagagatttc gtacaagggg aagatcccct tgcggcacag atgcctatcc ctgataaagt
    15181 ttgtgactga atatttcaga tatagacata taaggggaaa gaaagtgtga aatcaaaatt
    15241 tgtggagaga ttttatgatt agaaaagcaa tactaaaaga tgttaattcg attgaagata
    15301 cttacaatga gcatttccag tatgaactga atcatacagc ctttactgta tttaagaaag
    15361 gggtttatcc aacgaaagat gatgcagaac gggctattta tgctggtgct ttgtttgtat
    15421 atgaagaaaa cggaacaatt gttggaagta tcattattga taaagttcaa ccaatagaat
    15481 atgcaactat tccttggaaa gaaaaattat ctgaagatga ggtaatggta attcaccttt
    15541 taatggtacg tccgagtatg tctggtaaag gaattgcatc atcactaata aaattcgcta
    15601 cagaattagc acaaaaaaat agttgcaggg cactacgttt ggatacagga agtcaaaata
    15661 ttcctgcgct atctttgtat caaaaaaatg gatttgaaat aattgctagt gcttctaaaa
    15721 aagttggaga tgtaattgca cacaaaaacc atctttttct cgaaaagata ttgtagttcg
    15781 taaattccag tttgaaaaat tgagaaaatc ggagtttgag gagcatcacc gttatgatta
    15841 gagaattacg gaaagtggat ataaataaag ttgcagaaat atggttagat acaaatataa
    15901 agacacatta ttttatctct gctcaatatt ggaaaagtaa ttttgagtta gtaaaagaac
    15961 ttctattaca ggcaactgtc tatgtttacg aggataaaca ggaaatacag ggttttatag
    16021 ggttaagtaa cgaatacatt gaaggtattt ttgtttctgc cgaaatgcaa tctcaaggta
    16081 taggtaaaat tttgctaaac tatgtaaagg gaaaaagaaa caaattaatc ttaaatgtat
    16141 atcaaaaaaa tacacgggca atatcctttt atcaaagaga aggatttgag attcagtata
    16201 gcggtttgga tgaagctact ggagaaaaag actatgtaat ggcatggcag cagaaataga
    16261 aattcaagtt ttagtgtttg gaaaattatg acttagtgtg caagttggga gaaaatatgg
    16321 taacggaaaa agagaaaaaa gcatgtcgat ttattgccga atggctataa gattgtgaga
    16381 cagtctctgg tagatgatct ggagggcgaa aaaaaggtat gttgggagac agaaattttg
    16441 caggatgatg atagtggatt tacttatgaa atcagcaggt gtctgtattt cgatacctgt
    16501 aaggaacatg gctatgcaga gttctgcaaa gtattttgca accatgactg gtatgcttat
    16561 ggagtattaa agcatcattc gagatttatc agaaaatcga cgattgccga agatggaact
    16621 gtatgtcatg atacaatcga gaaagtaaca gagaaacatt ttttataaaa ccgaaaaatt
    16681 gattatggga ggaaattgaa aatggcgaaa gaagagagat ttgaaaaaat atattctcag
    16741 ggagcattaa ctactactga gatatgggtg gataaggaaa ctggtgtgaa ttatttatat
    16801 catgcatgtg ggtattcagg tggactgact ccattgttgg acagagatgg gaaaccagtt
    16861 atttctcagg ctataaatag atgaccttag aaacagcaac tgttatggta agtaacagca
    16921 tcgtcacaat atgtatatgg tcgattggtg tgtgcggtaa tatactatat tcagaaaaac
    16981 aattcaggag atttttatga gaagatcagg ttggacaaaa tggttgatcg tgattccggc
    17041 aatgctaggc attgtgttgg tgacctatgt gcggtataca acggatatct ggggatttgg
    17101 tgcatttatc tgccagctga ttgcaatttt ggaattggct tatggtctgc gaattgcgat
    17161 gctggcacag aatagaaaga agtcctatcg gttaacaccg gaagaacgac atgagtatgc
    17221 acggtattta tatgagaagc aatatcaccg ttaccctgct gtagcgaatc agatgctttt
    17281 ggttatggca cggatgagtg tactccttaa caattatgaa cgggctgcac aggagctggc
    17341 agatattcgt atagataaat tcaatccagc acagttgaag ttgtattatt acctgaaagt
    17401 ggttacagca atggcagcag gagatgcgac agggattcaa gagagccaga tgtgttacgc
    17461 agggattccg gatactaaat gcacatatcc gtcaaaagca gagcttgcta tgtggataga
    17521 gcagagagcg gcagcgcaga tggcagaggc actgaagaag gcagtgccgg ataaaaaaga
    17581 gcatccgata cggatcagta tcattacttt ggttcttgcg tatagtacct tcttctatgg
    17641 attgtggtat ggtataaaac gtgatatggg atatgaagtg agatatcgtt tcgcagaaat
    17701 atcgattact gttattacaa tcagtctggc ggttcttttg atctggggga tcgtgagttt
    17761 atacagacac aattgccagg cattatccat acaattcggt aaaaacaaag tggcattgat
    17821 aattgtctat ggagctgctg cgatatttgc acttttgatt attggaatga gtggtctgag
    17881 tgtagtgttc ggaatggatg gaacagaaac ggtgactggt tgtgacagca aatatacgta
    17941 tataactgtg caggaagatt atggtggtga acggagatac agaaccaata atccatttgt
    18001 tatgcagagt atggatggtc tgtggtggtc ttcggataca atagatgaag aagataatca
    18061 agaagaggat actacagaat taccggattt ggatacagag aattcaacag attcagagga
    18121 tattcagagt ggaaatacca tagaagatta cgagacagaa gggcagcatg atggtctgac
    18181 aattcaaaat gcaatgctgg cagtttataa ttaccttcag gagcagaata cacttcaaaa
    18241 tatggcgttc agttacacgg cgaatgcaaa gggagaagtc tatgctgtcg tcagcgaaac
    18301 gcaggaaaca aagaacggta atacggtaaa tgtgagatac tgtctgtacg ataatggttc
    18361 caagacagat gcagatggta attcctgcga agaactggtg ttagaaaaag tatatccgga
    18421 tggcaattac gagaccgaat tggtagattt ttatctggta aatgcagata cgatgcaggt
    18481 gacagacgaa cagaagaata cgtggtaaaa taaattctgg ggaagtaggt gcattgtatg
    18541 acaatgagag aaaaaataaa actattatcg taaatgtttc aagcggactg ggttcttttg
    18601 gaagtatgca aaaggactcc aagacataag aggtgaactg cgctgatcca ggtgccacgg
    18661 caacagattt gaatgtcgac ggaggaaagc aaacagtaca ggcgcaatcg taaaacttgc
    18721 aatgattgat aaaaccggac taacagtaac ttttattagt cgatttggaa atatgccttg
    18781 gtaatcaaca attccggttt tactgactaa gaaaatgagg aggattcata atgagtgaga
    18841 aaacagagat tacttttatg caaacaagac taatccggct tgcttcagaa gagtggcatt
    18901 tacctgttga acagattatc cacttgttta aagaagccga tgttcttgga tatatagaaa
    18961 aatgctatgg gatttttcat tgtgaaggcg atgaagctgt gcttgaagat attactgaat
    19021 ttttgcaaag aaaggggata gagattagtg cttgagttaa aagatggatt tgttctctat
    19081 catggaagtt attgtgaagt aaaagagcca gatcttgcca aatgtgcaaa gagaaaagat
    19141 tttggacagg gattttattt aaccacatca aaggaacagg cagaaagttt tctgagaacc
    19201 tcaatagcca aagccatcgc aaccggaaca attgaagagg ggcagaagtt tggatatatt
    19261 tcaacttttg aattcaaact ttcagaaaat cttgaaacgc atatttttga aaatgcagat
    19321 gtggactggt tacattgtat cgccgctcac cgaaagaaaa aaatgtttat tgaggttgaa
    19381 cgtgaaatgg caaaatatga tatcattgca ggaaagattg cggatgatgc aacaaacgct
    19441 actcttaccg cttatcttgc gggtgctttt gggactgcag gtgataaagt agcagacgat
    19501 ttctgtatca gacagttgct gccgaataaa ttgaaagatc aatactgctt taaaactgag
    19561 gcagctattg agtgtctgaa atttgtgaaa ggtgagaaga tatggctgaa gtaacaattc
    19621 caaaggaaaa aatgaattat acaatcgacc ttctgatcac tatggtgaca gatgaaatcg
    19681 cagaagagac aggcaaggac agaaacgagg tattgacaga ttttttgtgt tcaaagacag
    19741 gaaaagcact gtatgatgag aagacaaagc tgtggtgcac tggtccggca tatatagctg
    19801 aactgtatat ggaagaatta aagaaatcct gatatgggat gtgagtttaa atttatacga
    19861 caatggtgac aaagactttc attccaagga gcaacaatta aatatttcaa aaacgctgag
    19921 gtcagcattt cctaatcaca ggacaacgac ctcagttttt tatacccagg aatcggcatt
    19981 catgaataag ccggttcttt ttgtttacag aaaaaagaag gagtacaaga aaatgagcag
    20041 agcaaaaaat ccaaagtatg aatccatgtc attggaggaa ctgaaggcca acatggaaaa
    20101 gagcagacag gaactggagg cagcaattca ccacaaaaac attctggagc agcggaagaa
    20161 gctggtagaa cgcagggaac gttcccaccg gctgattgtc aaaggggcag agtttgagaa
    20221 agtatttccg ctgtccagag atctggaaca ggaagacgtc caggacatga tgaatcaact
    20281 gcagaacagc agttacaaca agagtattgt gcggcaggct catatagccg ccttacacaa
    20341 agatcaacag cagattgcag aagcagtaaa aaagacagag aagggagatg acagttaatg
    20401 gcactgtatc atttttcagt aaggaatgta tccagaggga aagggcagat ggtggtcgct
    20461 tccgcagcct atatttccgg gcagaggatt tttgacagtt attacaacaa gatacacgat
    20521 tatacgagca agtcaggtgt gatatttaca gaaatcctga caccggagta tgtgccagag
    20581 cgtctgactg acagggaaac actctggaac gaggtggagc atgtggaacg gaacaacaag
    20641 gcacagcttg cctattcgtt tgacattgca ctgcagaatg aattgacact tgaggaaaat
    20701 atcagactgg caaaagagtt ctgccaggag cagtttgtgg cacgtggcat gatcgtggat
    20761 ctggcagtcc atgagggaaa agcggaaaat gaagaagaac cggataatcc ccatttccat
    20821 gtgcttgtcc caatccgtcc ttttacagag gaaggaatct ggggcaataa gcagagaagg
    20881 gaatatattc ttgatgagga tggaaaccgg gtgaaggatg ccaaaggaaa agatatgttc
    20941 aatgctgttt ctactaccgg atggaatgat ccggagcttt taaaagaatg gcggcgggca
    21001 tggacagaga aagtaaatgg gaaattcagg gagtgccaca tggcatccag aatcgaccac
    21061 cgttcctaca aagaacaggg gattgacctg atcccgacca tccatgaggg atatgaagtc
    21121 cgggcaatgg agaaaaaggg aatcaaaacg gtgatcggag aattgaaccg ggcaatccgg
    21181 cagtttaacc agatgtttat ttctctgaaa gaatccattc agtggatgaa gacagcttat
    21241 gaggaaatga aagcagagct ggatcgcagg cagaacccga cactgctgga aagcctgcag
    21301 gattattatg ataaaaagac acagggcaga ccaccacttc ccaattttta tgcggagatg
    21361 aaacagagag gtaagaatct ctccaattta caggagtttg caaaatccat cagttatctt
    21421 cagacacatc agatagaaac gatggatgac ttgcaggaac ggattgagga actgaacggt
    21481 gttgtttctg tcagtaaaaa agaaatttct gaaaagcgga aacagttaaa ggaacttgag
    21541 aatctggaaa agatggcaga ggtaataaaa acaaatcagc cattgattga tgagtataac
    21601 catttctatt ttcagaaaag acgggagaga tattatcagc agcataaaaa agaaatcaat
    21661 tattaccgca aatgtgaacg ggaactgaaa caacatctgg ataaaaatgg aaaagtgcca
    21721 actgccagat ggaaacggga gaaagaagaa cttcaggcgg ggatcgaaga gctgaaagca
    21781 gataatcagc cgtaccagga ggaactaact tttgtgaaaa aggtacagag ctgtgcggat
    21841 attgccagac gtgaccggga aatggcagaa gcagatactt ccgggcgttc agaagaaaaa
    21901 agggagaagc aggtgaagtt cccgacattt tacacagttc aggcgaaaga gattcttgaa
    21961 gaaaacggga aagcagagca acagccgttg aaccagtcgg aacagaatcc agaaaagaag
    22021 acctctcttc tgagaaaact ggatgaaaaa aagaaagagt gtgcagagcg tgatgcaaaa
    22081 caacaggcag tgaagaaaaa gcgaaactat gacatgagtt tataacagtc tgtcagcagg
    22141 acagacggga aaggatgagc aaaatgcagt atacagaaga acaaattata agagcaaatc
    22201 agacagatct tgtttctttt ctgagcgcac agggagaaca actagggaaa agcggaaagg
    22261 aataccgttg gaaaaagcat gacagtgtaa cggtatccgg aaaccggtgg tacaggcaca
    22321 gtcaggggag gggtggctat ccggtggatt ttgtgatgga attttacaat gccacatttc
    22381 cggaagctgt caagatgcta accggggagg aaggcgaagg caggaacagt acctgccccg
    22441 ccccgtcccc tgacttccga ttaccagaga aagaggaaaa taatgataga atcatcagat
    22501 atctgacaga aaacagaggg attgaaaaga atatggtgga ggaatggatc ggcagtgggg
    22561 atatttatga agaaaagaag caccataatg tggtgtttgt tggcagagat gcagacggta
    22621 ttccaagata tgcccattgc aggggaactg gtgaaacaaa ataccgggga gatgtggctg
    22681 aatctgataa atcttacgga ttctgtcaca gagggacgga taatcagttg tttgtgtttg
    22741 aagcagccat tgatctgctt tcttttatcc aattatttcc caaagactgg aaaaagcgga
    22801 gctacctgag tctgggtggc atttccagtg cggcactgat ggcttttctt tctgaacgcc
    22861 ctcagattac ttcggttttc ctgtgcctgg ataatgacca tgcagggaat gaagccagtg
    22921 agaaattggc aatagaaatc ccggacggat acagtgtcat ccgtctgaaa ccgtcccgaa
    22981 aggactggaa tgaaatcctc tgtgataaaa atgcagacag gaagaaatca atcattgaaa
    23041 cagttacaat gaaagtaccg gagaaggaag aactggtgcc gatgttgtgt tatgaagaca
    23101 ttgaacagac cagtgtagaa tggctgtggt ttccctatct gccatttgga aaactgacga
    23161 ttattcaggg caatccagga gagggaaaaa cttattttgc catgatgctg accgcagcct
    23221 gcacgaaccg taagactttc ccgaacatgg aagagattga accttttaat gtgatctatc
    23281 agactgcaga agacggtatg ggagatacga ttaagccgag gctggtggaa gcaggagcag
    23341 acctgagtcg ggtgatggtc attgatgata cagaggaagc attgacgtta tccgatgacc
    23401 gtattgaaaa agcaatccgt cagaatcagg tcagactttt aatcattgat ccggtacagg
    23461 cttttatcgg tgccgatgtg gatatgaacc gggcaaatga agtccgtccg gtattccgga
    23521 aactgggaat gatcgctgaa aagaccggtt gtgcgattgt gctgatcgga catctgaata
    23581 aatcttcagg gacacagagt acttaccggg gacttggttc tattgatatc atggcggcgg
    23641 tgagaagcct gctttttatc gggaaagtaa agaaagaccc gacaacaaga gtactgatcc
    23701 atgagaaaag ttctcttgca cctccaggtg agacgatggc atttaaactt ggagatgaag
    23761 aaggattccg ctgggtggga gcttatgaga tttccgcaga tgatcttctg gacggaaagg
    23821 aaggaaagcc gacagaaaca aaactgcaac ggggaacgaa actgatctat gaactgcttg
    23881 cagacggaaa tgcagtcaca atccgggaac tggatgaaaa ggcaaaagca cagggaatct
    23941 cccagagaac catgcgagaa gccagaagcc ggatgaaaga agaactggat tatcggatga
    24001 atgaaaagca ggaaaatacc attcgattaa aaaaacaagg gagaatgggt gacggcagaa
    24061 ttttggagtg aacggcaggc tttatagata ccctatctgc cgtctgccgt ctgccggtgc
    24121 aaggcaatca aacggaaata gcaggtatcc atacagaata aggattgctg tgaatctgga
    24181 acaaaaatga attgtaatat ggcggatata atggtatgat tatccggtaa cagcaatgtt
    24241 acggggaagc acctgtgaca gggtgtggct gcctcctgat atttacagac cggagccttt
    24301 cggggttctg agatgaaaga aaggaggtgc gtgatatgac agcttatgag attatctcaa
    24361 tattcttagg cgtactcagt ttactgatcg cttttggtag cctgttaatt gcgattattg
    24421 cctttcttga tcggaagaac aagaggaaat aaaaatgcct atcctgtctg ccaacaggat
    24481 aggcggtggt cgtaagacca tttagtcaac tcaggcggca tccactttgg agtgggtgct
    24541 tttcctataa caaatgtacc acacattcct cttgttttca atgggaaaat taaaatatgc
    24601 agttcatctg acagggattt atccctgtct ttattatatc cccaatttct gaaatgttaa
    24661 tacaaaaatt ttaatatatg taaccaacag tctgggcagt taatctgtaa aaatggatta
    24721 gctgctcttt ttttgcaaaa aatggaggtt ttacaatggc agtgaagaag aaaattatca
    24781 acatcagagt atattttgac ggtgatcagg acgcaaaaga agtgtttgca gaggtgatcg
    24841 cacagaaaat acgtgacacg aaaacaaaag ataaacttgc aataatgaaa gataagcagt
    24901 ataatgatta tgagttcagt aatctgaaat ctgcgtagga tgtgcaaagt aacggaggta
    24961 tcagatgaac aacagatata tgagtaacaa tatagaaaga aatgcaacct ggagagttgg
    25021 tgtatactgc cgactttcca aagatgacga attgaccgga gagagtgcca gcatctccaa
    25081 ccagagagac atactggtga attactgtca gtcacagggc tggcaggtgg tggatgtgtt
    25141 tcaggacgat ggttatacag gattgaacac agaccgacct gatttgcaga gacttctcaa
    25201 agcctgtgaa aaaggactgg tgaatcttgt aattaccaaa gaccagagcc gactgggacg
    25261 aaaccatgta cagacaggat ttctgatgga ggagtttttc ccaaagcatg gagtaaggta
    25321 tatcgcatta tatgataatg tggatacctt tgccggagac aatgagattg ccccatttaa
    25381 gaacgtgctg aacgagatgt attccagaga tatttccaag aaagtccatg cgtcctatca
    25441 cctgcaggca accaagggaa agtttacagg tgttgttcca ccactgggat atcagaaaga
    25501 cccggaggaa aagggacatc tcctgattga cgacgagaca gcaccgattg tccggaaaat
    25561 tttccagtgg gcggtggacg gtcatgggat taactttata tccagagaac tggaacgcca
    25621 gcagattccc tgcccgtcat ggtggcaccg gaaacgggga ctccgcacct attacacaaa
    25681 atgggaaaag aaagacccgg aaaatgggaa atatgtatgg gatgaatcct atctgaaatc
    25741 cctgctgatg aatcccgttt actgtggtga catggcttcc cagaaaaggt actaccgttt
    25801 caagattggc aatcagggag ataaagctcc ggaggactgg atcacagtca gggataccca
    25861 cgaagcaatc attccgcagg atgtatttga tctggtacag gcaaagatga aaggcaggaa
    25921 acgccagaca gaacagggag aatacagcat gtttgccgga ttactccgct gtgcagagtg
    25981 tggcggtgcg ttgacgttga agaagacgca caccaaagac cagcatgaag tttatacctg
    26041 ttccacttat atccataagg ggaaagcaca ttgtacccag caccgggtgg acgctgatga
    26101 cctgtatgat gcagtcctta ccagaatcca ggaatgtgca aaagcagtta ccggagacgg
    26161 aacagaactg gaaaaccggg taaaggagtt atgcgaggaa gatacacagg gacacaggga
    26221 aagtctggag aagctggtat caaagcagaa agaccgcctg gaaacgttag accggctgat
    26281 tgcaaaactg tatgatgacc ttatcaatga caagattaca gaatctgttt ttgataagat
    26341 gctggagaag acacagaagg agcagacaga tatcaaaaag gaacttttcc agaatgagag
    26401 tgtgctgaat acggaagaaa aactggatgc acagtcccag cagtggattg acgatattag
    26461 cgagtatgcg gacatcaaag aactggatgc caatcttctg aaccggctga tcagtaaaat
    26521 cgttatctca gaaccgcagg agaaagacgg aacagaaaat tactgccgta ctcctgaaac
    26581 ggtcacaatg gaaattcatt tcaacttaaa accaataccg gagcttggaa ccatcgaaag
    26641 aggttctggc tcccataaat aagaaaaatc tatcaaaatt taatctattt taatcttttc
    26701 aaggctcatt tcgagcaaat attttctcat aaaagacata aaacagcttg tcgctggcgg
    26761 cggcgtggcc ctgatcggtg tcactctggt tcccctgctg tccgggctgc tgggctgatc
    26821 ctggcctttg tccctgtcca aacccttaac taatcgcctc cgcgtcctcc cggccagacc
    26881 gggagggcgc gggaaaggag gtgctacccg tgattagtga aatcattgaa gaatggataa
    26941 aaggcatcct catggacggt atcacgggca acctgtccgg gctgttcgat acggtaaatg
    27001 ccaaagtcgg agaaatcgcc gccgacgtgg gacgaacccc gcaggcatgg aacagcggta
    27061 ttttcaatat gctgcacagc ctgtcggaaa ccgtgatcgt ccccatagcc ggggccatac
    27121 tcgcccttgt catgtgctat gagcttatcc agatgataac ggaaaagaac aatatgcacg
    27181 actttgacag ctccatgttc ttccgctgga tattcaaatc cgcgtttgca atcctgatcg
    27241 taacgaacac atggaacatc gtcatgggcg tattcgacgc cacccaaagc gtcgtcaatc
    27301 aaagcgccgg tgtgattatc gggcagacat ccattgattt taacaccctg ataccagacc
    27361 ttgaaagcca attggaggct atgagcgtgg gcgctctttt ggggctgtgg ttccaaaccc
    27421 ttgttgtggg cctcaccatg aatatccttt ccatctgcat tttccttgtg acctatggcc
    27481 gcatggtgga aatctatgtt gtcacgtcgc tggggcctat cccccttgcc acaatgggca
    27541 acgcggaatg gcgcggcatg ggccaagggt atctgaaatc cctgctggcg ctgggcttcc
    27601 aagccttttt aatcatggtt gtggtgggta tctacgcggt gctgatacag aatatcggtt
    27661 ccgcttccga catatccggc gcaatctggg gctgtatggg ctatacggtg ctgctgtgct
    27721 tctgcctgtt caagaccggc agtatatcaa aggccgtatt cggggcgcat tgatcccaaa
    27781 atccaagcat acacaggagg aacttgttta tttcaacgta caacatcatt tacgctgacc
    27841 cgccgtggcg gtatgactgc aaaaacgtac agggcgcagc ggaaaatcac tattccacca
    27901 tgagcattga cgagctgtgc gccctgccgg tggaacggct ggcgtccaaa gactgcctgc
    27961 tgtttttgtg ggccacattc cccatgctgc cggaagctct gcggttaatc aaagcgtggg
    28021 gcttttcatt caagaccgtg gcttttgtat ggctgaaaca gaaccgtaaa agccttacat
    28081 ggttttacgg gctgggccgc tggacaaggg gaaacgcgga aatctgcctg ctggcaacac
    28141 acggcaagcc gaagcgtcgt tctgcctctg tccatcagtt tattatcagc ccgattgaac
    28201 aacacagcaa aaagccggat gtgacccgtg aaaaaatcgt agagctggcg ggcgatttgc
    28261 cccgcgtgga gctgtttgcc agacagaaaa cccccggctg ggacgtgtgg gggaacgagg
    28321 tggaatgtga ccttatcctg acgtgaaagg aggtgccaaa tggcttatgt aaccgtcccc
    28381 aaggacttaa cccatgtgaa aagcaaggtt gtgtttgggc ttaccaaacg gcaactgatc
    28441 tgtttttccg gggcgctggt gatcggcgtc cccttatatt ttctcacccg tgactatctt
    28501 accaacagcg cggcggccct gctcatggta tttgccatgc tgccgggctt tttgcttgcc
    28561 atgtacgagc gtcacggcca gccccttgaa gtggtgatta tgcagatcat ccagtgctgc
    28621 ttcatccagc caaaggagcg tccctatcag accgacaaca cctacaccgc ccttgtgcgg
    28681 caataccaga tggaacagga ggtaaaggcc attgtccaaa aaggaaatcc caagagaaac
    28741 cgaaaaaaca aagctgaccc gcgcccagcg aaaagaaatt gacgcggtaa tccgtaaata
    28801 taagggcgac ggcaggcccc acacggcgca gcagagcatc ccctacgagg tcatgtaccc
    28861 ggacggcgtg tgccgggtat cgcccggcgt cttttccaag tgcattgagt ttgcggacat
    28921 cagctatcag cttgcccagc cggacacgca gacggctatc tttgaaaaac tgtgcgacct
    28981 gtataactat gtggacgcct ccattcatat ccagttttct ttcctcaatc ggaaagttga
    29041 cccggtgcag tacgccaaga gctttgagat cgcgccgcag ggggacgact tcgacgacat
    29101 ccgggccgag tacaccggca ttctgcaaaa gcagcttgca aacggcaaca acggcatggt
    29161 aaagaccaaa tacctgacat tcaccatcga ggccgaaagc gtgaaagcgg cgcgagccag
    29221 attgaagcgc atcggctttg acctgttagg ctatttcaag agcatggggg ccgtagccca
    29281 tgtgatggac ggctgggaac ggctgaacct gctgcatggc gtgtaccacc cggacggcga
    29341 gatattcaat tttgactgga agtggctggc cccgtccggc ctctccacca aggattttat
    29401 tgccccgtcc tccctgtgct tcggcaatgc aaagacattc ggcatgggcg gcaaatacgg
    29461 cgcggtaagt ttcctgcaaa tcctgtcccc ggagctgtcg gacgatatgc tggccgactt
    29521 cctcaatacg gaaagcggtg ttttagtcaa tctccatgtg caggccattg aacagaccaa
    29581 ggcgatcaag accatcaagc gcaaaattac cgaccttgac gctatgaaga ttgcggagca
    29641 gaaaaaggcc gtccgcagcg gttacgatat ggacattctt cccagcgacc ttgccaccta
    29701 cggcgaggac gccaagaagc tgctgacaaa gttgcagacc cgcaatgaac ggctgttcca
    29761 gttgacgttc cttgtcctca acgtggccga caccaagcaa aagctgaata acgacgtgtt
    29821 ccaagccgca ggcgtggccc aaaagcataa ctgtcccctt gtccggctgg actaccagca
    29881 ggaacagggc cttgcaagca gcctgccgct gggcgtcaat cagatcaaga tacagcgcag
    29941 ccttacgact tccagcgtgg ccgtgttcgt cccctttgtg acgcaggagc tttttcaggg
    30001 cggcgcggcc atgtattacg gcatcaacgc caagtcccgc aacatgatta tgctggacag
    30061 gaagcaggcc cgttgcccca atgccttgaa gctcggcacc cccggcagcg gcaaatccat
    30121 gagctgtaaa tccgagatcg tcagtgtgtt cttaacgacg cctgacgata tttttatttc
    30181 agacccggag gccgaatatt acccccttgt caaacggctc cacggacagg taatccggct
    30241 gtcgcctacc agcaaggact ttgtgaaccc gctggacatc aacctgaatt attcggagga
    30301 cgataacccg ctggccctga aatccgactt tgtgctgtcg ttctgtgagc ttgtcatggg
    30361 cggcaagaac gggctggaag ccattgaaaa gaccgtgata gaccgggctg tgcgcgtgat
    30421 ctaccggccc tatctggcag acccccgccc ggagaatatg ccgatcctgt ccgacctcca
    30481 caaagcgttg ttagatcagc atgtcccgga ggctgaccgg gtggcgcagg cccttgacct
    30541 gtatgtgtcc ggctccctga acgtatttaa ccaccgtacc aacgtggaca tcggcaaccg
    30601 gcttgtgtcc tttgacatta aggagctggg caagcagctc aaaaagctgg ggatgctcat
    30661 tgtccaagac cagatatggg ggcgcgtcac ggccaaccga agccaaggaa aggccacatg
    30721 gtattttgcc gacgagttcc atttgctttt gaaagaggaa cagacggcgg cctattctgc
    30781 ggagatttgg aagcggttta ggaaatgggg cggcattcca acaggtgcca cccagaacgt
    30841 caaagacctg ctttcctccc cggaaattga aaatatcctt gaaaacagcg acttcattac
    30901 gctgctcaat caggcgtccg gggatagaaa aatccttgcg gaacgcttga atttgtccac
    30961 cgaacagcag aagtacatcg acaattccga acccggcgag ggccttttga tctttgaaaa
    31021 cgtggtgctg ccctttacca accccatccc gcacaacacc cagctttaca agattatgac
    31081 gacccgctta aacgaggtgg cgggagtatg acccggcgac gccacaaagg aggtgaacca
    31141 ccatgaaaga gctgaaagcc aaggcgaaag tgacgcaggc catgacccgc gacgggctga
    31201 cgctggaaaa tcaggccacc ggcgaggtgg agaacatttc cagccgggag gccgaacagg
    31261 atttgtccgc gccggggccg ggcggcaccg ccgataagct gctggaacgg gccgacgcca
    31321 tccatgacgg ccataaggcc aagaaagccg cgaagcaggc cggggctgtg gccgccgagg
    31381 gggacgctgc cctgcaccgg ccatccgccc ggctccaact gtcggaggaa gaacgggccg
    31441 acccggattt acagccctat atccgaaaat cggacaaacg ggccgataag ctggataagg
    31501 cccgcgccgc catccccaag aagcgcgtcc cggtcaagga aaccgtcttt gacgaggcaa
    31561 gcggcaaagc aaagaccgtc ctgcactttg acaaacagga caaggggcct ccctccctga
    31621 aacccaatcc ggcaggccgt cctgtttccg aggtttttct attcggccac gggaaaatcc
    31681 atgaagtcga gcatgaaaac gtgggtgtgg agggcggcca taagggggag gaacttgccg
    31741 agcggcaggc cggaaaagcc atccgcagca ccatccggca ccacaggctg aaaccctact
    31801 gggacgcgga aaaggccgag cgcaggctat tgtctgccaa cgcggagtat ttctaccaga
    31861 aatctttgcg ggataacccg gagatcgcac aggcggccag caaccccatt tcccggttct
    31921 ggcagaaaca gcagatcaag cgccagtacg ccaaggccgc ccggacagcg ggccagaccg
    31981 caggcgcggc ggcacagggc gcggccaaga cagccggggc ctccacgaaa gcggccaagg
    32041 ccgcagccaa agaaagtgaa cgggcggcct cctttgtggc ccgccactgg aaaggggcgc
    32101 tggccgtcct tgtgttgggg cttttgatgc tgcttatcat cggcggctta cagtcctgca
    32161 ccgccatgtt cggcagcgcc ggaagcggca tcacggcgtc ctcttacttt tccgaggata
    32221 gcgatatgtt ggccgccgag gacgcctacg cggagctgga agccgagctg caagactatc
    32281 tggacaacta cgaggccacc catgactatg acgaatacca ttttgacctt gacgagatca
    32341 gccatgaccc ctatgtgctg atttccattc tgtcggcgct ccatgacggg atatttacca
    32401 tcggggaggt acagggtgat ttgtccatgc tctttgacaa gcaatatatc ctgacagaaa
    32461 acgtcgtggt agaaacccgt taccgcaccg aaaccgatac atggacggac gcggagggca
    32521 acacccatac agacacctac caagtgccgt atgactatta catctgctac gtcacgctgg
    32581 aaaacttcga cctgtcccat gtgccagtct atatcatgga cgaggaaacg ctaagtttct
    32641 attccctgta tatgtccacg ctaggaaacc ggcctgatct gttttccggc agccagtacc
    32701 ccaacgcctc caccctgacg ccgccgacct actacgacgt acccccggag gctttggagg
    32761 atgccacctt tgccgccatg ctcaaagagg cggaaaaata tatcggttat ccctatgtgt
    32821 ggggcggcag ctctcccagc accagctttg attgcagcgg cttcatttcg tgggtaatca
    32881 atcatagtgg atggaacgtg ggacggctgg gggcgcaggg cctatataac atctgcaccc
    32941 ctgtttccag ttcgcaggcc aagcccggcg accttgtatt tttcaaggga acctacgaca
    33001 cccccggcgt gtcccacgtc ggcctgtatg tgggcaacaa tgtgatgctg cattgcggga
    33061 acccgatttc ctacacgaac cttacttcaa actactggca atcccatttt tacagttacg
    33121 ggcgtttacc ttaacgccag aaaggagctt tcaattatgg caaccaaact tgaccgcatc
    33181 gaaaaggaca tccagaaaac caagtctaag atcgcggagt ttcagaaaca gcttcgggag
    33241 ctggaaacgc agaaaaccga acaggagaat ttgcagatca tccagcttgt gcgcggcatg
    33301 aacatgaagc cggaggaatt tgccgcgttc ttacgcagcg gcgcaatgca ggccgcccct
    33361 gccgcaaccc cttaccatga acaggaggac aatgcccatg aagaataaga ccagacggat
    33421 ttcccgtatt ctccccgcac ttctggccgt gatgctctgc gtcacggcct ttcccgttac
    33481 cgcccttgca ggcggcaatg acccggcacc ggccccgctg ccggaggcta caaccgaacc
    33541 cgctacgggc ggtttggagc ctgaaacaga cgaaaccggc accgccacgg acgggacaga
    33601 aaccgaggcc gaaaaagata ccggccctct gaccggcaag gacatcaccg acctgttttc
    33661 cgcgctgttc ggttccaagg tgagcattgc cgccacggac gacggcatcc agatcaccac
    33721 cggcaccgac aaaaaagaac ctgaacagac cggcaccgtc accaccaacg gcggcaggct
    33781 gaatgtccgc accggcgcgg ggcttgacaa gaccgcgttc acccagcttc ccaatgggac
    33841 gacggttgag gtagtcggca cagacggcga ctggattaaa attctcttgc cggagcgcat
    33901 cggctatgtc cattccgact atatgaccgt cagcgaaaag gaagtagcgg ctaccggcga
    33961 gggcggtttt tccctctcca ttgacccgga ggaaattgct tctctgctgg aactgttcaa
    34021 tggcggcggg gccagcgccg ccctgtcccc ggacgggaac ctgtcactga ttgacgacat
    34081 cggcagcgcc gcgaaaaatg gcaagcagtt tatcaccgtg gaaaccaaaa acggcaatgt
    34141 gttttatctg attattgacc gggacgacga gggaaaagaa actgtccatt tcttaaatca
    34201 ggtggatgaa gccgatctgc tgacgctgat ggaggacggg gaaaccgtca cccccgctgc
    34261 tgtctgctcc tgtaccacga aatgcaaggc cggggctgtg aacacgaatt gcccggtatg
    34321 taaaaccaac ctgaccgagt gcagcggccc ggagccgcag gaaccccagc cggaggaacc
    34381 cgaagccccg gaggaagaac ccaagggcgg cgcgggcggc ctgatcgtgt tcctgctggt
    34441 ggcgctggcg ggcggcggtg cggcccttta ctatttcaag ttcaaaaagc ccaaggctga
    34501 aaccaccggc agcgacgatt tgggcgagta tgattttggg gaggacgagg atttggagga
    34561 cgaggaaccc gaaaccgagt ttgacccgga agctgatttt gtgagtgaac aggaggacga
    34621 ggaatgacat ataaacttgt aatcgcggaa aaacccagcg tggcccgcag cattgcgggc
    34681 gtgatcgggg ccacggagaa acacgacggc tatttggagg ggaacggcta tctggtgagc
    34741 tggtgcatcg gacatctggt gtcctttgcc gacgccggac gctatgacga gcgtttcaaa
    34801 aaatggcgct atgaggattt gcccattctc ccggaaacat ggcagtacat cgtccccgac
    34861 gacaagaaac agcagtttga cgttctctgt tcgctgatgc agcgccccga cgtgaccggc
    34921 cttgtgtgcg ccaccgacgc cgggcgcgag ggggaattga ttttccgttt cgtctaccag
    34981 atggcgggat gccaaaagcc ctttgagcgc ctttggattt cctctatgga ggacagtgct
    35041 attaaggacg gctttgccca cctgaaaccg ggcggcgact atgatccgct gtatcaatcg
    35101 gccctgtgcc gggccaaggc cgactggctg atcggcatta acgccacccg gcttttttcc
    35161 gtcctgtacc acaagaacct gactgtgggc cgcgtccaga cgccgacctt aaaaatgctg
    35221 gtggacaggg acgccaagat taccgatttc aagaaagaga aatatcacat cgtccatatc
    35281 acggcgggcg gcgcggacgc ggtaagcagc cgcttttccg acgctgccga ggcaaacacc
    35341 gtcaaggcgg cttgtgcaaa agcgcaggcc gtttgcgttt ccgtgacgcg ggagaaaaag
    35401 acggaacagc cgcccaagct ctatgacctt accaccttgc agcgggaggc taaccggctt
    35461 ttcggctata ccgccaagca gacccttgac tatgcccagg cactgtatct aaagaagctg
    35521 ctgacctatc cgagaacgga tagtcaatat cttaccgacg atatgctgcc cgccgtggaa
    35581 agccttgtgt ccggcctgtg ggagctggtg ccgtttgcca agggcctgaa cctttccccg
    35641 cagtttgacc gcatcctgaa cagcaaaaag gtgtccgacc accacgccat tatccccacc
    35701 gctgaatttg tgaagcaggg ctttggcact ctggccgaga gtgaaaaaaa gctgctgtcc
    35761 ctgatctgct gcaagctctt atgtgccgtg gccgggccgc atatctatga ggccgtcacc
    35821 gccacattta cctgtgccgg gcaggagttt accgccaagg gcaagaccgt cctttccccc
    35881 ggctggaaag agattgaacg ccggttccgt tcttccttaa agaccgacgc cgacgaggac
    35941 gccgaggcgg tgcgggagct gccggagctt tgcgagggcc agacctttgc agacgtggcc
    36001 gcctctgtca ccgagcattt caccacgccg cccaagccct acaccgaaga tacgcttttg
    36061 tccgctatgg aacgggccgg ggccgaggat atgccggagg atgccgagcg caagggatta
    36121 ggcacaccgg ccacccgcgc cgccattctg gaaaagctgg tgcagatggg ctttgtccag
    36181 agaaaaggca agcagctcat tcccaccaag gacggcatca atctggctgt ggtgctgccg
    36241 gaggcgctta cttctcccca gcttactgcc gaatgggaaa accggctgac cgagatcgcc
    36301 aaggggcagg ccgatcctga cgaatttatg gacggcatcg agacgcaggc gcgggagctg
    36361 gtgcagacct attcctgtat cagtgaggaa aagcagaaat tgtttcaggc cgagcgtgtt
    36421 cctatcggga cgtgtccccg ctgtggtgag gctgtctatg agggcaagaa aaactactac
    36481 tgcggaaacc gggcctgcca gttcgtcatg tggaagaatg accgattttt tgaggaacgc
    36541 aagaaagcgt tcaccccgaa gatcgccgct gcgctgctca aaaccggcaa agtcaaggta
    36601 aagggcctct attccgtaaa gaccggcaaa acctacgacg ggacggtgct tttggctgat
    36661 accgggggca agtacgtcaa ctaccgcatt gaacacagga gctaaaggac agtaacacag
    36721 caagcatagg gagtgtggct acactcccgg taaaatcccc cggccgcgtc cctgccgggc
    36781 cggggatttt tgcttgttgg ggaagtcccc aaccccctat tttttgaaac agaacagaga
    36841 aaggagtgac cgcgtgaacg aagtattttc tatccaagtc cataaccggc agtttttgga
    36901 acagggcaaa caaggcgtct ggctggattt gcccaccaca accgaaacgc tgcaagccgc
    36961 cctgcgggat gttggcatta ctgccgacaa tccgcaggat ttttttatca acgggtattc
    37021 ctgcccggag gacagacacc ttgccattcc ctatgacatg gtgctggccg ccgacgtgga
    37081 cgagctgaat ttccttgccg cccggctggg ccagcttgac gccgccgaga tcgcagagct
    37141 gaacgccgcc ctgcaaaatc cccgtggcgg ctttgagaat atcggccaga tcatcgacta
    37201 caccgaaaat gtggattact atgtgcatct tcccgacgtt aggggggatg gacagttggg
    37261 cgactactac ctgaaccgtt ccggcatggt ggatatgccg gaggaatgga aagccgggat
    37321 tgatacggcg cagtttggac gccacattgc ccagcaggaa caaggcgttt ttacccagta
    37381 cggctatctg gtgagaagcg gcgacgaatg gcagcgcgtc catgagggcc agccggtgcc
    37441 ggaggaatac cgggtgatgc actaccccca gccggaagct gaccgggagg aaccgcaggc
    37501 ccagcagccg gaggcatccg aaagccccga caaagcgccg cccccggtta tcccgatcat
    37561 gctggacggc atgaacagca aagaacgcat gaaagtgatt actgaccgtc tggaaaccgg
    37621 cgtacaggag ctttttgaaa gtagccgcta tatggcttac ttatccgcta tgtccaagat
    37681 gcacgattac agtctgaaca atacgctgct gatcgccatg cagggcggtt cgattgtggc
    37741 gggctataac aggtggcagg atgaatttga ccgccatgtg aagcggggcg agaaaggcat
    37801 taagatactg gccccggccc cgttcaaggt taagcagcag atggagaaaa tcgacccggc
    37861 cacccagcag cccgtgatcg gcgcggacgg caagcccgtg accgaagaac aggaagtgac
    37921 ggttccggcc tttaaggtgg tgacagtctt tgacatcagc cagaccgagg gcaaggattt
    37981 tcccatgccc tatatcagtg aacttaccgg cgacgtgcag cagtatcagg atttctttgc
    38041 cgcgctggaa cggacatcac cctatcccat aagcattgag ccgatttcag acggggccaa
    38101 gggccgctgc ttctacgcaa aacagcgcat tgccatcaac gagggcatga gcgaattgca
    38161 gaccctaaag accgccatcc atgagattgc ccatgccaag ctccacgcca ttgaccggga
    38221 ggcccccgcc gaggaacagg cggcccggcc agaccgcagc acccgcgagg tacaggccga
    38281 aagcgtggcc tataccgtct gccagtatta cgggctggac acgtcggact attcttttgg
    38341 ctatgtggcc gattggagca gcggcaggga gttatccgag ctgaaagcct cccttgaaac
    38401 catccgggcc acggccagcg aattgataac cgagatcgac ggccattttg cccagcttca
    38461 gcaggagcgc gaggcccggc aggaacagga ggccgtcacg gttgacgtta ccccggaggc
    38521 agcggccccg gcgcaggccg aggaagaaca ggccccggcc cttgatccgg cagccgagcc
    38581 gcttgtgact gtgctatgga gcgaaagccc ccagctaaag gacggccagc aaatgccgtt
    38641 gcatgaggcc gacgcgctgt ttgcccgtct ggatgcagca tatccggcag aggcaggcta
    38701 tgacaaaacc gcgttccgca ttgactgtat ctttcagggc gagccggaca gctacgaggg
    38761 gcggcaggat tttggagata gggacggttc cctgatcgaa catatccaga gctaccacca
    38821 attttacgcg caggacgaaa actggaaaaa gcatgtaatc gctacaaccg gcatagaggg
    38881 ctgggagcag gacaaggccc atcgggatat gctgttaggc gagtttgtcc cctatatgaa
    38941 gctgcattgc agccttgccc aaatggaaca gacggcgcag ggccgcttac agtccgggga
    39001 aaccctgacg ccggaggaaa cggcctattt gggggcgctt ctctcttatg tgcaggagtg
    39061 ccgccccatg ctcaatcagg gccagtatca gttgccggag ccgccgaaac tggctgactt
    39121 cgaccagagc ttgcaggact acaaagcaca ggtacaggcc gagatcgcgc aggaggccgc
    39181 cgcagccggt atgaccgtgg aggaatacgc ggcggctggc tatgaagcac cccaacagga
    39241 caccttttcc atctaccagc ttcgaggcgg ggaggaaacc cgcgactatc ggtttgagcc
    39301 atacgagcgt ttacaggccg cagggctggc cgtggacagt gccaactatg accttgttta
    39361 tacggcaccg ctggataaaa gcacgtcttt ggaggatatt taccgcacat tcaacatcga
    39421 ccacccggca gattttaggg gacacagcct ttccgtttcc gacgtggtgg tgctgcacca
    39481 gaatggacag gacaccgccc actatgtaga cagcttcggc tatatggaag tgccggagtt
    39541 tttgcgggaa aatcccctgc gtaccgccga actgtctacg gagcagaacg aaaacatgat
    39601 tgacggcatt atcaacaata cgccgcccga accgcagccg gaggaacccg aacagctatc
    39661 ttttgaggat gtggcagttg cttccggcca gacacaggag gaacccccgg aggctgcgcc
    39721 cccgcaaacg gctgtccgct attatcccat caatgaggga gccgcccgcc gcgccaagga
    39781 agccatgagc ttttctgact accggcccgg cagcgccacg gcggaatacc ggcactatgt
    39841 cgatcaggcc gtggaaattg cggagcgcca gaaaaagcgt gtggagccag agtatcacga
    39901 aaagatagac agcctccttg acacctacgc ccgcaagctg gccgagaaca tgaataaggg
    39961 ctatgagatt accgcccgcg tcccctctgt cctgattgcc gggccgtcca atttcccggt
    40021 acgggcgaaa gaaaagcaga acgccgccag tgaccgcaac atggaggaat ttcagtacat
    40081 tcagggcttg cttgacaaaa tccgcagcac cggcatgggt ggtatcagcg ccgacgatcc
    40141 gcaggccgtt tccaagctgg aaaagaaact ggaaaagctg gaagcgtcgc aggagttgat
    40201 gaaggccgtc aatgcctact accgcaagca tgggacgctg gacggatgcc cccatctgac
    40261 cgaaagaggc attgaaaacc tgaaagcgga tatggcgtcg ggctggcact atgagaaaaa
    40321 accgttccag tcctggcagc tctccaacaa taacgccgag atacgccggt taaagggccg
    40381 cattgaggaa ttgacccgcc aaaaggaggc ggcctatgtg ggatgggagt ttgacggcgg
    40441 cactgtggag atcaaccggg tggccaaccg cctgcaaatc ttctttgagg ggaagccgga
    40501 tgctgctgta cgcgacgagc tgaaaagcaa cggtttccgc tggtcgccca aggccggggc
    40561 atggcagcgg cagttaaacg acaccacaat ccgcgtcgct gaccgtatca agtgcatcca
    40621 gcccctttcc ggcgaaaagc ccagcgcctt acagcttgcg gcccgcaggg agaaagaaaa
    40681 gccctctatc cgggcgcagc tcaacgcggc caagaccgaa caggccaagc agccaccggc
    40741 gcgggaaaaa tcaaagggat tggaggtaga gtaaatgcag aagtttgaaa atgtggacgt
    40801 tctggccgcg ctggaacaga tcatgcggca gaacaccgcg ttttatcaaa gtgactttgg
    40861 cattgacaaa gacattatca ggcgggatgc cgccagcgat caggccgcag acaagaccct
    40921 gctctggatg tcccggccct ccgggacgta ctgtttccgg gaacgtgatg ttttcctgaa
    40981 agacacccga cagtacaaca catggaaatt ttacggggag cagacccgtg acaagatact
    41041 ggcctacgcg gtggagctga ccggcacaca gggcggcact atccggggca atctctatga
    41101 gctggactat ccgcagcatt tccggcacgt cattgaaacc gcccagccgg tgagtgtgaa
    41161 ccggctcttt tatgagcatg gcacccgtga catcccggag agccagcgtt ttgacggaag
    41221 ccctgaccgg gtattgggaa actttttgta ctatgaggcc cagccccatg acccggctgt
    41281 gctgcaggag gcgctacgga cggagcagca cagccggggg cgggccgtcc ccggcgattt
    41341 caaagtccac gtcaccgctt tgcatgacag ccggattgaa accgaggccc ggcgtattgt
    41401 ggacagcctg aaagatctgt ccgcgcctaa cagccccaat aaaacgcatt tcatggtgga
    41461 gatctccccg cactttgcgg cgattgcttc caccaaggat aatgaccggc tctttgccat
    41521 gctgccctat aaatccctgt gttttaccgg gatgaaaggc aggcatggcg tgtatgccgt
    41581 gattaaccgg gacgagcgcc gggatgtgag tattcgcagg ccccggccct ctatccgcaa
    41641 gcagctatcc gacagcaaac aggccgccag cccaaaaaag gcggcggcca gaaccaaaaa
    41701 gaatgaattg gaggtataag gaatgatgca ctttaccgtg gaggaagaaa acctgatctg
    41761 tatgtaccac aatgcagacc ggcgcagaac gataaacagg ctcaccgccg tcatgccggt
    41821 tatggatggg gatatgcggg cgcaggccca gcagaccctt tccaagctgg aacgcatgac
    41881 ggatgccgac tatgacggcc agaagttcca cttcacagac gaataacgaa cagcagacgc
    41941 cgggcgcggc ggccatgtgg tcgcttgcgt ccggcgtctt ttctttttgc ctattcctac
    42001 gcatttagaa cccggtattg tcggggatag gatgaagtat tcgcttgccc ctgcaaacat
    42061 gcgggagaag ccttgcagcg ggcggttttc aatcccttaa atgcgtaggt tgcagctata
    42121 attagacccc tgaaacagat atagccgcgc agcgggtgta tctgtttcag gggtgtgcag
    42181 gtgatagccg caaagcggcg taagagggtg cagccctctt gccgtctatc tctgttccag
    42241 cgggaagctc ggcaatgctt ccagcagttt cagggaaatg cgctgccgta tatcttcgtc
    42301 aatgactttc ttctttttgc cgtttgcctg tctgatctcc accgtggaaa gctcgttgat
    42361 gtagtcagca taatgggcca acactttttc cgtggcccac ctctcaccgg caacggcggc
    42421 tttgatcgtt tcatagtcca gcagcttttc ttgtccgtcc atcctgatac ccccgtcaga
    42481 ctgatttttt ctatgatacc atattcggaa gcatggaacc agagaaaacg gcgcttttct
    42541 gctggtaaat tctacgcatt tagaacagca cttcggcggg gataggataa aacacccaca
    42601 taccccgctg accatgccgg aaaagccttg aaatataagg gcttttacag ctctaagtgc
    42661 gtaggtttcc agtatctttt ccgctggcgc tcggcctctt tgcggcggcg catccgtttg
    42721 gagcaggccg cgcagtattt ggcccggtta gacttcggcg tgaacgtggc cccgcactca
    42781 cagcagcgtt tcacttcgtc ccggtctttg gtgatctcgg catacagggc cgcatccagc
    42841 gggaggacag cggccttaaa ccagcggcac agaagcgaat aggaaatgct ctgtacgcaa
    42901 atacattctt cgccgtcgtc cagcaacagg cagttgccgt cacaatagtt gcagcactca
    42961 tgcgtcagct tccgggcctt gcgatattga gggtaattca ttcgtggaat actcatagct
    43021 cttgcgtcca ttcctttccc ggcgtcatac gcaaaatgcc gtccacattc tgcttgatga
    43081 tgccgtattc ctgcatctgc tttttcgctt cgccgtactt ctcatacagc cgttcctttt
    43141 cctcggccaa tcgcttgtac tcggctttca gggcgtacag atcggggagc tttttcacac
    43201 cctgtccctt tagggccttt gccgcagctt catacaaaat caacgtgctt tcatgctcac
    43261 gccggaattt tgccttatcc gctgcattgc ggtattccat agccacgggc cagagctgct
    43321 tgtaggtaga aatattcttt atcagcaccg ccatatccgc aagccggtgt tccatagctt
    43381 tgagggtagc ggcggcctcg tcgctggcgg cggtgatctc ggccactttg ctttccaaat
    43441 ccgggtaggt gtctatccca tgttcagtca ggaaattcaa agtctttgcg gcctgcttca
    43501 aattgtggag cttcgcccac ctctcatagc ctgccgactg ctgggcctta atgttgtttt
    43561 ccagatcaat ccgcaaagaa atccccttgc gctctttggg cgctctggtg ataacgcggc
    43621 ctttgatacg ctctttgatg gcttcctcgg tgtaggcttc ccccaatgtc ttgcagcggg
    43681 taaagcgttc ctgtccggga gcgcggaaag aaataaactt tcccggcttg atctcatagc
    43741 cctgcgcctg catgagccgc aggaaatcat caaaatcttt tgcctgcggg atagcggcgt
    43801 caatggctgc tttgagcttc gctttccagc tcgtcccttt ccgctgggcg tcccactcgg
    43861 catagctctt gcccttactc tggccgggct tcaccacgga caatccgttt tccttgcaca
    43921 gcctgtcgct ggtgcgccgg atataggcat agctctgttt gttggaaatg tacttgcgct
    43981 gatttaccat atccacggca cagaaaataa tgtgattgtg gacgtggccc ttatcaatat
    44041 gggtagtcag cacataggga tatttccctt gcagcacttc ctcggcaagc tgcttcccga
    44101 tctcatgggc ctgttccggc gtggcttcgc cgggggcaaa ggattgtatc aggtggtggc
    44161 ccagattatt gcccttttgg tatgcctgcg ccagcagcat ttcaaattca atgtccgctg
    44221 tttcataaga gcaggcaaag gaggccacca gcagcttgcc gtcagttttg tccgggttct
    44281 cgatgtagtc aagggctttt ttcagcgttg acttgatagg atgtatcttt gtaactgcca
    44341 tatctgcgcc agcgccccct ttatatcctc gatgtcctgt gcgtacaccg gccccatacc
    44401 gttcagcctc tttgcaatct gattgacgtt gacgccgatc ttctgtatct cggcggtctg
    44461 cgcccggatg tcgctggtgt ccatgttgac gatatagccg tcaatgagca tcttccgggc
    44521 gtaggctgaa aagttcttcg tatgcagcag cgccattttc tgttcaatca tagcccgttc
    44581 ctccggcgtc acagggacgc gcagcacgat ttttcgcttt ctgtttgcca tgcttcaccg
    44641 tcctttccat tcaggggttt ggggatttcc ccaacaagca aaacggacgc ggcccggcag
    44701 ggacggggca ggccgttttt ccgggagtgg ctacactccc tatgcttgct acgttactgt
    44761 ttctccacta acaagacgtt tccaaagccc caaactaccc gcaaaatagc cctctggaaa
    44821 gaaaaaagtg caaaaaaata gaagctacgc tgtgcagctc ccgctaaaag taaaacagag
    44881 gatgccagtt acacatcctc cgtttcattc ttcttcggct tttcaattag tatggcgctg
    44941 tcaatcgcgc cctcgatgat cggcaggtat tcttcggggc agagtttcag cttgtggctg
    45001 accctttgcc gcagttcgct ttcctcccgc atgatctccg ggttaaagta gcgttccaca
    45061 ggaagcccgc aggctttaat aagctgtatg acaaccggca gactgggaat ggccccgtcg
    45121 ttttcgatgt tggcaagata gcgccagtca atatttattt tctctgccat tgctttgcgc
    45181 gaaacgcgct ttgcttgccg ggccgctttc acatccgcgc cgaaagtttc aaagccggga
    45241 caatcttcaa tattcgccat atcgcacctc ccacttacat tgtatatttc atactctgcc
    45301 cgtggaatgt tggtatatgc acctttctta aagtttatat ttcacatttt gtttctttta
    45361 ttcttcgagc aaaagaatta taatgtaccc tgtggaggtg tactatggac tacatgacgt
    45421 tgaaagaggc aggcgagaaa tggggtgtgc ccccacgatg gataaactac tattgtgctg
    45481 cgggacgcat ccccggcgct gtaaagatgg cgacgatctg gctcattccc aaaactgcgg
    45541 aaaagccgat tgacaggcgt acaaagaaag gaaaggcggt tactgaatag tataatgaaa
    45601 ccggcgcatt ttggatatgg ttttgaagag agagaaagca tggtattttg tcacggaaaa
    45661 actataagat gatagatggc aggctgctcc agaccgataa aaagtattct catctgaaac
    45721 agaaagaaaa gatttacaca tggatgtatg aagaagcgaa actttatcat gacagaaatg
    45781 gaaaatgccc gaaacaaaga cgaggaagct gctgctgtat cagccgtata tggtagaatt
    45841 gaaaaggccg gcatctggat tctttatggt gaagtctcca gacattggat aactgctggt
    45901 ctaatgcgcc tttgagtatg gggtgattga ttttaatacc ggggaggtac ggctttttga
    45961 ggaaaatatt ctgggattta catttggcga ttattacatt tcctgtaact gcaaggacac
    46021 ggatgatgaa gatatgagga atatagactt gctgattttc tactgtcttg aacggtatta
    46081 ggactgaatc tgctgcgcgg agtgcggcaa gagcgaccgg agggagtaca cacaggccct
    46141 gcctgtgtga aaagccatac cgcaaggtgc attcgcaaaa aagaggccgc gcattttgct
    46201 gaggcttggg gtgtccccaa caagcaaaac gagaacgccg caggcgggca agtggtggat
    46261 cagaatatcc gtaaaggata ttctgatcca ttgcttgcca gtattatcgg gatgttttga
    46321 caaccgctgt aatcgaaaca tcccaaacat taagaaggtg ttaattatta ttatcttgat
    46381 ttaattcgtt taaaccaata atatagatac atacaccatg aaaagaagcg agtatcccaa
    46441 tgacggatga ttaaaatccg aaaagaaatc gctttgaaac tacagggaaa ctacagactg
    46501 ttatgttatc ttcttaaaat ggagggattt ttatgtcgat acgaattcta cttgtcgagg
    46561 atgatgatca tatctgtaat acagtaaggg cgtttctggc tgaggcagga tatcaggtgg
    46621 atgcctgcac agatggaaat gaggcataca ccaagtttta cgaaaacact tatcaactgg
    46681 ttattcttga tattatgctg cccggtatga acgggcatga acttttgcgt gaatttcgtg
    46741 cgaaaaatga tactcccatt ctgatgatga cagccctgtc ggatgacgaa aaccaaatcc
    46801 gggcgtttga tgcagaggca gacgactatg taacaaagcc attcaagatg cagattttac
    46861 taaagcgggt ggaagccctg ctgcggcgca gcggtgcgct ggcaaaggaa atccgtgtcg
    46921 gcaggctgac acttctgccg gaggatttca cggtactttg tgacggtaca gagctgcccc
    46981 tgacacgaaa agaatttgaa atccttttgc tgctggtgca gaacaaaggc agaaccttaa
    47041 cgcatgaaat cattttgtca cgtatatggg gatatgactt tgagggtgat ggcagcacag
    47101 tccacactca tatcaaaaat ttgcgggcca agctgccgga aaatatcatc aaaaccattc
    47161 gcggtgtagg ataccgattg gaggaatcat aataatggaa agaaaaggga ttttcattaa
    47221 ggttttttcc tatacgatca ttgtcctgct actggttgtc ggtgtaacag caacactgtt
    47281 tgcacagcaa tttgtgtctt atttcagagt gatggaatta cagcaaacag taaaatccta
    47341 tcagccattg gtggaactga ttcagaatag cgataggctt gatattcaag aggtggcagg
    47401 gctgtttcac tacaataacc aatcctttga gttttatatt gaagataaag agggaagcgt
    47461 actctatgcc acaccaaatg ccaatacatc aaatagtttt agacccgact ttctttatgt
    47521 ggtacataga gatgataata tttcgattgt tgctcaaaac aaggcaggtg tgggattgct
    47581 ttatcaaggg ctgacaattc ggggaattgt tatgattgcg ataatggttg tattcagcct
    47641 tttatgcgcg tatatctttg cgcggcaaat gacaacgccg atcaaagcct tagcggacag
    47701 tgcgaataaa atggcaaacc tgaaagatgt accgccgccg ctggagcgaa aggatgagct
    47761 tggcgcactg gctcatgata tgcattccat gtatgtcagg ctgaaagaaa ccatcgcaag
    47821 gctggaggat gaaatcgcaa gggaacatga gttggaggaa acacagcgat atttctttgc
    47881 ggcagcttct catgagctaa aaacgcccat cgcggctaca agcgttctgt tggagggaat
    47941 gcttggaaat atcggtgact acaaagatca ttctaagtat ctgcgcgaat gcatcaaaat
    48001 gatggatagg cagggcaaaa tcatttccga aatactggag cttgtcagcc tgaatgatgg
    48061 gagaatcgta cccatagctg aaccgttgga catagggcgc acggttgccg agttgctgcc
    48121 cgattttcaa accttggcag aggcaaacaa ccagcggttc gtcacagata ttccagccgg
    48181 gcaaattgtc ctgtccgatc cgaggctgct ccaaaaggca ctatccaatg tcatattgaa
    48241 tgcagttcag aacacgccgc agggaggcga ggtacggata tggagtgagc ctggtgctga
    48301 aaaatgccgc ctttttgttt tgaacatggg cgttcacatt gatgatactg cgcttccaag
    48361 gctgttcacc ccattctatc gcattgatca ggcgcgaagc agaaaaagtg ggcgaagcgg
    48421 tttaggactt gccatcgtac aaaaaacgct ggatgccatg agcctccagt atgcgctgga
    48481 aaacacctcg gatggcgttt tgttctggct ggatttaccg ctcacatcaa cattgtaaat
    48541 atttaaaact taaatgattt tgaccgacag gtataaccct gccggtcttt ttttgctgct
    48601 acaggaaaac tacagactgg ctacagggaa agtacagatg cgcttgccat aataacaatc
    48661 gtaccagcca caaatcgtag ttttattgca aaggaggcat tcgatcaaat ggaaaaaagc
    48721 aactatcatt ccaatgcgga tcatcacaaa cggcatatga aacaatccgt ggaaaagcgg
    48781 gcttttctat gtgcgttcat tatttcgttc acagtctgca cgctgttttt gaggtggaga
    48841 ctggcttccg tattggaggc aacacagata ccgcccatcc ctgcaactca tacaggcagc
    48901 agcactgacg tagtggagaa tttggaggaa aacgctcttg ccaccgccaa agaacaggga
    48961 gatgaacagg aatggagcct gattttagtg aacaggcaga accccatccc cgcacagtac
    49021 gatgtggagc ttgagcaact atcaaatggt gagcggatag atattcggat ttctccctat
    49081 cttcaagatt tgtttgatgc cgcaagaact gatggagttt acccgattgt cgcatccgga
    49141 taccgaacaa cagaaaaaca gcaagaaatt atggatgaaa aaattgccga atataaggcg
    49201 aaaggctaca cctctgcaca ggctaaagcg gaagcagaaa cttgggtggc cgtgccggga
    49261 acgagcgagc atcagcttgg tcttgctgtg gatatcaatg cggacggaat tcattcaaca
    49321 ggcaacgagg tttatagatg gctggatgaa aacagctatc gttttggttt tattcgtcgc
    49381 tacccaccag acaagacaga gataaccggt gtgagcaacg agccgtggca ttaccgatat
    49441 gttggcatcg aagctgccac agagatgtac aaccaagggg tttgccttga ggaatattta
    49501 aaaccagaaa aatgagaaaa ggatgttatg ttatggacag aaaaagattg acacagcgtt
    49561 tcccgttcct gcttccaatg agacgggcgc agagaaaaat gtgcttttat gcgggaatga
    49621 gatttgacgg ctgtcgctat gcgcagacga taggggaaaa atcgctttcc catttgctct
    49681 ttgaaacgga ttgcgcatta tacaaccaca ataccggatt tgacatgata taccaggaaa
    49741 acaaggtgtt caacttaaag ctggcagcaa agaccttaaa cggcttattg ataagaccgg
    49801 gggaaacctt ttctttctgg tggctggtac gccatgcgga caaagatacc ccctataaag
    49861 acggccttac ggtgaccaat ggtaaactca ccaccatgtc gggcggcggt atgtgccaga
    49921 tgagcaattt actattttgg atgttcctgc atacgccatt gacaattatc cagcgcagag
    49981 gtcacgaagt aaaggagttt ccagagccaa acagtgacga gattaaaggg gtggatgcaa
    50041 ccattccaga gggctggatt gatttaaaag tgcgaaacga taccgactgc acctaccaaa
    50101 tatgggtgac cttagatgat gagaaaatca tcggtcaggt gtccgccgac aaagagcccc
    50161 aagcattata caaaattaca aatggcagta tccagtatgt ccgtgaaagt ggcgggattt
    50221 atgaatatgc ccaggttaaa cggatgcaag ttgccttagg taccggggaa ataatagatt
    50281 gcaagctgct ttatacaaac aaatgcaaaa tctgttatcc cctcccggaa agtgtggata
    50341 ttcaggagga gaaccaatga gaaaaagtgt gggcattact gtttttggat gcgaacagga
    50401 tgaggcaaat gctttccgcg ctttatcgcc ggattttcat attatcccta cgctgattag
    50461 cgatgcgata tcggcagaca acgcaaaatt ggccgctggc aatcaatgcg ttagcgtagg
    50521 ccataagtcc gaggtttccg aggcgacaat tcttgcgctg agaaaggtcg gggtaaaata
    50581 catttctacc cgcagcatcg gctgcgatca cattgatacg actgccgccg agagaatggg
    50641 aatctcggtt ggcacggttg cgtattcgcc ggacagcgtt gcggattatg ctttgatgct
    50701 gatgctgatg gccatacggg gtgcaaaacc caccatgcac gccgtggcgc aacaagattt
    50761 cagattggat cgtatccggg ggaaagaact gggggatatg actgtgggag ttattggaac
    50821 cggccatatc gggcaagcgg tcgtcaaaag gctgcgggga tttggatgcc atgtgctggc
    50881 ctatgataac agccgaaaaa tggatgcaga ttatgtccag cttgatgagc ttctaaaaaa
    50941 cagcgatatt gttacgctcc atgtgccgct ttgtgcggat acccgccatc tgatcggtca
    51001 gaagcaaatt ggagagatga agcaaggcgc atttttaatc aacaccgcgc gcggagcgct
    51061 tgtcgatacc ggggcgctgg tggaggcgtt ggaaagcgga aagctgggtg gtgcggcact
    51121 ggatgtgttg gagggcgagg atcagtttgt ttataccgat tgctcgcaga aagtgcttga
    51181 ccatcccttt ttgtcgcagc ttttaaggat gccgaatgtg atcatcacac cccatacggc
    51241 gtactacact gagcgtgtgc tgcaggatac tacagaaaaa acaatcagga attgtctcaa
    51301 ttttgaaagg agtttacagc atgaatagaa taaaagtcgc aatcatcttc ggcggttgct
    51361 cggaggaaca tgatgtgtcg gtaaaatccg caatagaaat tgctgcgaac attgatacgg
    51421 aaaaattcga tccgcactac atcggaatta caaaaaacgg tgtatggaag ctatgcaaga
    51481 agccatgtac ggaatgggaa gccgacagtc tccccgccat actctccccg gataggaaaa
    51541 cgcatgggct gcttgtcatg aaagaaagcg aatacgaaac acggcgtatt gatgtggctt
    51601 tcccggtttt gcatggcaaa ttcggggagg atggtgcgat acaggggctg tttgtattgt
    51661 ctggtatccc ctatgtgggc tgtgatattc aaagctccgc agcttgcatg gacaaatcac
    51721 tggcctacat tcttacaaaa aatgcgggca tcgccgttcc cgaatttcaa attattgata
    51781 aaggtgacaa gccggaggcg ggtgcgctta cctaccctgt ctttgtgaag ccggcacggt
    51841 caggttcgtc ctttggcgta accaaagtaa acggtacgga agaacttaac gctgcgatag
    51901 aagcggcagg acaatatgat ggaaaaatct taattgagca agcgatttcg ggctgtgagg
    51961 tcgggtgtgc ggtcatgggg aacgaggatg atttgattgt cggcgaagtg gatcaaatcc
    52021 ggctgagcca cggtatcttc cgcatccatc aggaaaacga gccggaaaaa ggctcagaaa
    52081 atgcgatgat tacagttccc gcagacattc cggtcgagga acgaaatcgg gtgcaggaaa
    52141 cggcaaagaa agtatatcgg gtgcttggat gcagagggct tgcccgtgtt gatctttttt
    52201 tgcaggagga tggcggcatc gttctaaatg aggtcaatac catgcccggt tttacatcgt
    52261 acagccgcta cccacgtatg gtggccgccg caggaatcac gcttcctgca ctgattgaca
    52321 gcctgattac attggcgtta aagaggtgat aagtatggaa aatggttttt tgtttttaga
    52381 tgaaatgttg catggcgtcc gttgggatgc caagtacgcc acatgggata acttcacagg
    52441 aaaaccggtg gatgggtatg aggtgaatcg catcatcggc acaaaggccg tggcgtttgc
    52501 tctgcgcgag gcacaaatcc atgcggctgc gcttggctat ggcttgcttt tatgggatgg
    52561 atatcggcca agaactgcgg tggactgctt cctgcgttgg gcagcgcaac cggaggacaa
    52621 tctcacaaaa gaaaaatttt accccaatat agagcgagcc gagttgatta caaagggtta
    52681 tgtggcctca caatccagcc atagccgtgg aagcgcaatt gatcttacgc tctaccacct
    52741 ggatacaggg gaacttgttt caatgggaag taacttcgat tttatggacg aacggtcgca
    52801 tcatacagca aaagggatag gggatgcaga ggcacaaaat cgaagatgct tgcgtaaaat
    52861 catggaaagc agcggatttc agtcttatcg ctttgaatgg tggcactata agttgattga
    52921 tgagccatac cccgatacct attttaattt tgccgtttca taatgaaagt attggttttg
    52981 tatcagattt tggatttgat aaaatctatg ctggtcatgg acaatctttt actggaaagt
    53041 ggttaaagca tcttttgtaa tacattgcaa attatagtat tcaatctgcc cagcggcaga
    53101 aaagaaaaaa accgctgttg ggcagacctt tcccacgccc attttgtatt ttgctggcgg
    53161 cggttttgag aaatcaaggc cgccgttctt ttctggcagc aaaaggtgtg gcggcccata
    53221 ggaggatgcc gctatgctgt tttgctattg ccatgctcta atccacttcc accactcaaa
    53281 aaagccttgc ccctccacag ggctattccg gttatgagta tgtactatac catgtaaatc
    53341 agcagcatag caaagcattt tctcgcccgg taggtttgcg acctgccggg cgatttttgt
    53401 cgtcttttgc ggcccggctg tccccaaaaa aattttttga gaaattttca aaaaggaggt
    53461 atttagcggg tagctggcgg ccttgtcaaa gtcttgttag cggaaaggga gagaaccacc
    53521 taagtccccc acagaaaggg ggtgagaaga tggatgctat cccccgcgac tatgaagcgc
    53581 ggtgcatgtt cgatgctttt tgcaagacgg tactgcgtaa cgaggccaaa agctacttgg
    53641 ctgaaatgaa gcgccgccgt gaccgtgaag tgtcactaag ctctttgtca caggcagatt
    53701 tggacaagct ctgtacagta gatcactacc ccagcgacag ctttacgttt tcgtcgcatg
    53761 gctatgacct acatatcaac aatgagcttg ttgccgaagc ctttgccgcg ctgcccagca
    53821 tggagcagag tattttaatc ctgcactgtg ttctggatat ggcagatggt gagatcggcg
    53881 gccttgtggg aatgtcccgg agtgccgttc agcggcacag gacaaatacc ttatcggagc
    53941 tgcggaaaca gctaaaggcg ttgatgccga agggaggtta gacgcggatg aagaacccga
    54001 actacaaggg cattgagctt ttgccccttt cggtgatcga ggccgcccgc gctggggatg
    54061 ccggagccgt agagcgcgtt ttgcagtatt acagcggtta catgaacaag ctgtgtaccc
    54121 ggacgctcta tgacgaaaac ggctatccgc atgtgtgtct ggacgaggcc atgaagcgcc
    54181 gtctggaaat caagctcatc cacgctatcg tcacgaactg acgaaccggc cccggcagca
    54241 tgaaacggct taccctttcc ccgtttcggt gttttcccgg tggcctgatc gcaccttgat
    54301 aaatatgccc gcaagataac ggcggcgcat catagggcat acggacacat tctgtctgtg
    54361 ccgagccgga cggctggtgc gccatgatac ttttccctac gggaaaagcg gagcgaggaa
    54421 caccacgccg caatgtgagc gtggcggctt gcgggcgacg acgtacaggc aggactatga
    54481 tactccctta tagccacagt ccgagcgtta aaagcgtcgc aggcaatggg tagggccgcg
    54541 tgagaaccac gcgggggtga aagtcccgtg gagctgtgcc agcagccgtc cgtctatgcc
    54601 cctaaatcac acaaaccttt gaatttgtga aagggggctg cattatgaac catgatgtgc
    54661 ctatttggga aaaatacacg ctgacgattg aggaagcgtc taaatatttc cgtatcggag
    54721 aaaacaaatt gcgtaagctg gccgaggaaa atccggcctc tggctgggtc attatgaatg
    54781 gcaaccgcat ccagatcaaa cgcaagcagt ttgagaaaat aattgacact ttggacacaa
    54841 tctaatagtg aaaaagggcc gcagatatgt tataataatt tataagcata tatgcggctc
    54901 tttccgtcat ggaaaggagc atgacgagat gtcagaaaaa agacgagaca gcaaaaatcg
    54961 cgttttgcgg tctggagaga gccagagaaa agacggacga tatgcttaca aatatgttga
    55021 tacctttgga aaaccgcagt ttgtttactc atggaagtta gtgcctacgg ataagacccc
    55081 ggcaggtaaa cgggaggata tttcactccg ggaaaaggag aaagaaatcc agaaagacct
    55141 tgacgacggg atagacacca tcggcaagaa aatgaccgtc tgccagctct acgccaagca
    55201 gatcaggcac cggggcaatg tgaagcacaa caccaaactg ggccgggaac ggctgatgcg
    55261 tattctggaa caagatcggc tggggagctg cccgattgac agtgtgaagc tgtccgacgc
    55321 aaaggaatgg gcgcttcgga tgaaagaaaa gggcctttct tataagacca tcaataacga
    55381 caagcgttcc ctgaaagcgg ctttttacac cgccatacag gacgattgca tccgaaaaaa
    55441 cccgtttgac ttccagctta gtgacgttct ggacgacgat acggaaccaa aggtgcctct
    55501 tacaccagca caggaagaaa gttttctgtc ctttattcaa ggtgataagg tttaccaaaa
    55561 gcattatgac gcaatcgtta ttctgctggg gacagggctt cgcatttcgg aactgtgcgg
    55621 actgacggac aaagatttgg attttgaaaa ccgggtaatc atcgtgagcc accagctttt
    55681 gagaaatacc ggcgtcggtt actatattga cgaacccaag acccaaagcg gcgtcagaaa
    55741 aatccccatg aatgaggaag tgtatcaggc gttccagcga gtgataaaaa accgcaaagg
    55801 tgcaaagccg ttcattattg acgggtatgc caatttcctg tttctcaaac agaacggcta
    55861 tcccatgacg gcggtagatt atggcgggat gtttggcagg cttgtgaaga agtacaacaa
    55921 gagccatgaa gaagcgttgc cgaaaacgac gacgccccat gctatgcgtc atacattctg
    55981 cacccggtta gccaatgcgg gaatgaaccc caaagccttg cagtacatta tgggacattc
    56041 caatatcacc atgacgctga actattatgc acacgccaca ttcgattccg caagggcaga
    56101 aatggaacgg ctggcagcct gacacaaggc cggttttact actccattta ctacttttga
    56161 gggggaaatg gtgagaggta ataagaacat ttgtaagtat ctaacaatat caaaaatgcc
    56221 ggaaaagccc ggaaacacgg gctataccga cattcaagaa cttctaaaaa gataatctaa
    56281 aatcccatat aatttttcct caaaagctga tacaaagact tatattgagc ctggagtagt
    56341 aaaaaagaga aatattatcg gtagtcccat ctttcagatt ccatatattg gactagtcct
    56401 ttcaaaaacg gcatttgcat ggattcaggg gttattcttt acgattgctt tttgcctaac
    56461 aggaatcaca gcatggaata cctttgtaga gttgaaaaag aagcggagac gaacacagaa
    56521 atttttgtaa gaatgttaaa aacataaatg tattccagag atccaacgac tcttctgaac
    56581 atgaaacacg agaaaacact catgcagctt gtagatttag tcagttgttc gtataatgtg
    56641 tgaagaggag gagtagtatg cgcaatgaaa tgatgcatcg tcctgtagtc aaagaagaac
    56701 tattggattt tatgcgtacc cagcaaaaaa aattaccggg tgagctaggc aaactggaag
    56761 aagaagctca tgtagcagaa gtaccagtta ttccccatga aacagttgta tttttgaaat
    56821 ttttacttgg acagcttcag cctgaacgaa tcttggagat cggagcagct atcggttttt
    56881 catcaagtgt catggccacg acaatcggag aaaacgctca tgttacgaca attgatcgtt
    56941 tcgatgtcat gatcgagaaa gctaaaaaaa actatgaacg tctaggactg acagataaag
    57001 tgactttgct tgaaggtcaa gcagcagata tcttgccgga gttgtctggt ccgtatgact
    57061 ttatttttat ggatagtgca aaatcaaaat atattgaatt tttacctgaa tgcttacgtt
    57121 tgttaagaaa aggtggcgtt ttgatggtag atgacatctt ccaaggagga acgatcttgc
    57181 tcccagatga agagattccc cgtggcaaac gagctatcca tcgtaaatta aatgaatttc
    57241 ttcgtgttgt gatggatcat cccgatttga catcgacaat cttgccatta ggtgatggtg
    57301 tgatcttaat gacaaaggaa tcagaaacaa tcgagttgtg atacatgatt atggcagcct
    57361 gtagataatc gattgaatcg agggctcaat tcgaacttga tgtaaaagtt tctatatttg
    57421 taagtcaatt aaaggataca ggaaatctaa ataaacagga gtagcatgaa gtcaatgtag
    57481 ctcctgtttt caattgataa ccttagaaca gctgaaggag aatgtctcgt tttaaaagat
    57541 tgatttagta gttgggccaa aataattcat tatttgctaa ttaaataact atttcgtatt
    57601 gacagaagca atttaattat ctataattat tgatgttccg ctgattcttg tcatagtagc
    57661 tcagctggat agagcatccg ccttctaagc ggacggtcgg gggttcgaat ccctcctgtg
    57721 acgctaaaaa accgttagaa actgaggttt ctaacggttt ttctttgttt tttgtgccaa
    57781 tattgtttgt tttcaaaggc aaataaaata aaaaaacatc cttatcgagt acaattataa
    57841 ggagcaaaaa aataaaactc atcagaaaag aggaagaaga tatgacgata ctatctagaa
    57901 ttttagtgac tctagttgca attgaatttt tttatatcat gtatatcgaa acggtgagaa
    57961 ctgattctga cacgacaagt agggttttta aaatgagtaa agaagagtta agcagaaaat
    58021 ctgttcaaac attgtttaag aaccaaggag tgtacaatgg actattagga gtgggactac
    58081 tttatggagc ttatctttct tccgcatcta aagaaatcac aagtatgcta ttaatatcta
    58141 ttttttttgt tgcattatat ggcagtttga ctagcgatcg ttggatttta ctcaaacaag
    58201 gtgggctagc tctgttggca ttgctttcta ttttcttata aatatagaaa aaataagaat
    58261 ttatctaaca ggaaactttg tgaaaaatat tactttatct catgaaatat gatattctaa
    58321 tgaatggtat tttagaaaat ttcatgaaaa aggagttaga taggtgttca aaaattatgt
    58381 ttcctcacta aaaaaagaat tttcaggtta tgatctttca agatttcaga aagatctttt
    58441 ggctgggatc acagtagctg ctgtagcatt gcctttagcc ttggcttttg gggtatcaag
    58501 cggagctgat gcggccgcag gtttgattac ggctatcatt gccggtatcg ttattggcgg
    58561 tttatcaggc ggtttttatc aaatatcagg tccgacaggt gcgatggcag ctatcttgat
    58621 gtctattgcg gcaacaaagg gaatgcaagg tgtattgctt gctacttttt tagcgggtct
    58681 atttttactt gcagcaggac ttttccgatt aggaacactg acttctttta tcccagcacc
    58741 cgtaattacg gggtttactt caggtattgc tatcattatt gcattaggtc agatcgataa
    58801 tttatttggt gttcactcgg aaggtacaaa cgttattgaa aaactaagta gttatcaaac
    58861 gataggattt ccgataaaca cagcctcttt gatcatggga gggctagtca ttttagggat
    58921 gtttatctat ccaaaaaaat gggcgaaacg aatgcctagt tcgctgctgg ctatcatttt
    58981 ggcaacagca gttatgctat tgttccattt accagtagca actgtgggta agattccaca
    59041 aacattgatc agtagtaatc gtttgaatat gggagatttt agcttttctg ccttgcaaca
    59101 agtggcagtt ccagctatta gcattgcttt attaggaatg attgaaagct tgctatgtgg
    59161 tgcttcggct ggccgaatgg caaataagcc cttagatagc aatcaagaat taatggcgca
    59221 aggaattgga aatctgttgc ttcctttctt tggcgggatt cctgctactg cggccatcgc
    59281 ccgtacaagc gtggccatca aatctggtgc acaaactaga ttagctggta tgatacatgc
    59341 agttgtctta ctgctctcca tgcttgtttt tgcgccagtg atgtcacaga ttccaatgcc
    59401 agcacttgca ggtgttttga ttgtgacggc ttggcgtatg aatgaatggg aaacgatcaa
    59461 ggagctattt actaataaat attggtcagc agttctattg tttatcctta cgatgatttg
    59521 tacagttatc tttgatttaa gtattgctat cgtgatcggg gtgatcggcg gctgtgtatt
    59581 ttttgttgtc aaaagcgctg ctatcacgat ttcattagaa acgatcgatt ggcaaagaat
    59641 ggggctgcca gaaacggaaa agctgaataa ctggacagta gtgtatatta gtggtccact
    59701 gttttttatg tcttcagaaa aactcaaaaa taaattggaa gaactaaatc atcacgatgg
    59761 tgtcattttc tccatgcgag gcgtaccaag tattgatttg acggcacgtg cagtttttga
    59821 tgaatttcaa gaaaaagcag agcaaagagg acaaacgatt atctatactt ccttgcagcc
    59881 agaagtagaa aaacaacttt ctcacttgtg ggaacataca ttcacgcatc aacatcaaac
    59941 agttgctcat gcgttgacag ctttacatgc gaaatatact gcaattagcc attaaaacaa
    60001 tgaaaaagaa cagacaagac tgtaaactga agattttatg gtcttgtctg ttctttttaa
    60061 tattgctgta aaagtgaagc tagtacttgc cagtcttgat ggataacagg tacaagggag
    60121 cgattgtaga aaatagctgc tggatgaaaa agtggaagaa ttcgatagtt tttttctgac
    60181 cattcgtagg catccttttg ttctgttaat tgtaagacag gccctgtata gagttgacca
    60241 tgatttttgg taaccgtata ttctttgcca agtaaacgtt gaagaccaat gtttccaagt
    60301 gtcgcaatta attttggctg gaccttcatc agttcgtaat ccaatattgg tgcatgcgca
    60361 aatacttcgg aacgagtagg tgtccgattt ggataaacag tttctgactg ttgtgtccgt
    60421 ttattgatcc gatgagtcac gcgatacggg cggctcctta cagcactggt gatatacacc
    60481 tcttctcgtg ttaagccagt tgaagaaagt gcctgcatca attcttttcc agcttgaccg
    60541 ctaaaaggaa catgcgtatc aatctctgtt ttccctgggg cttctcccac gagcatcagt
    60601 ttaggatgaa gaggtccttg cccagcgata aatccttcta caggacggcc gttcattctt
    60661 aaagaagctt cttgtatcag ttcttttggg tattccattt atgctcctcc tttttctttt
    60721 attataagaa taaaagaaac acaatgcgac tattttattt ggtagaactg tgaggataga
    60781 aaagcagaag tattgcttga atttttgttt tactttttga ttttaaattt agttgactaa
    60841 cggttgctgt tctggtaagc tagtactgtt gtaattgtgg actccacagc tacaaccgct
    60901 cagaacaagt aataagcaca gcatatgctg tcacttcgga tggcgagtct aagtatatca
    60961 gaaggaggtg ctttatagca tgtacgcaat tattaaaact ggtggtaaac aagtaaaagt
    61021 tgaagtggga caagcaatct atgttgaaaa acttgacgtt gaagcaggag aaaaagttgt
    61081 ttttgacgaa gttatcttag taggcggaga atctacaaaa gtaggcgctc caactgttgc
    61141 aggtgcaact gttgaaggta ctgttgaaaa acacggcaaa caaaagaaag tcgttacttt
    61201 caaatacaaa cctaaaaaac attcacaccg taaacaaggt catcgtcaac catatacaaa
    61261 agtggttatc gacgcgatca acgcttaata gcgtttgaga aagaaggcct atatatgatc
    61321 aaaggaacat ttaagcgaaa tgacgcaggt cagatcgttt cattcacttt gaccgggcat
    61381 gctgatgctg gtccttatgg aagtgatatc gtttgtgcgg gtgtttctgc gttagcgatc
    61441 agcactgtca acgggattgc ctcattagcc ggttttgagc cgattgttga aatgaatgaa
    61501 gaagaaggcg gttatcttta taccgaagtc acttcaggaa tgacacaaga acaaaacaat
    61561 attgctgaga ttctattaga aaatctttta ttaggattac aatcgataga agctgaaaac
    61621 tcagaatata tccaaattaa aacaattaat gataaatagg aggtgcagac catgttgtta
    61681 tctatgaatc tacaattatt tgcccacaaa aaaggtggcg gttctacatc taacggccgt
    61741 gactcagaat caaaacgtct aggtgctaaa cgtgctgatg gacaaacagt tacaggtgga
    61801 tcaattcttt accgtcaacg cggtacaaaa atctatccag gtgtaaacgt gggtatcggt
    61861 ggagacgaca ctttatttgc aaaagttgat ggcgtagtac gtttcgaacg taaaggccgt
    61921 aacaaaaaac aagtgtctgt ttatccagta gctcaagaag cttaattgaa tcttatgatt
    61981 aaagaggttg tgacaagact tttgtcacag ccttttttgt ttataaaaat aaaagttgta
    62041 gtggaggcta cgaagtttac tttgtctttt tacacttaat atttttttgc caatttggaa
    62101 tcaggatgtt ttgataacta tcgttttcaa ttgatggaag ctatattgtt tgctatcagt
    62161 gaaataatta tatggtaaac agtgttcttt ttcttgtttc cattataaaa atcgtatcgg
    62221 gatttttcgt tcttttaaat tgaacatttc tatctatccg cgtgttatca acaaaacaaa
    62281 atcaggtaaa tagttgacga aacagaaata aatacatagt attatgtttt gaagcctaag
    62341 tacaatagtc tatagaaaag gagcgatacc attggcaaaa cgcattgacc gaatttatat
    62401 gtatgtcaaa gaaaaaaccg ctcatttaac aaaggcagaa tatgatcaag gagtaacaac
    62461 acaggaaatt gctgaagtgt tagggattca gcgaacaaat agcagtaaag atctgaatca
    62521 gctagtgcgc gaaggaaaac tgataaagac agacggacga ccagtacgtt acatatatca
    62581 aacactcgtt acgcacgtaa agccagttac aaaacatgta gtcagttaca aagaaacgcc
    62641 ggtctctgaa gaaaaaaata cactccaaat agatactaag gatatctttg caaaaatcat
    62701 tggagctaac ggcagcatga aaaattcagt ggaacaggca aaagctgcga ttttgtatcc
    62761 gccaagagga ttaaattgtt tgattacagg acctaccgga tcaggaaaga cttattttgc
    62821 acacgcgatg tatcattttg caaaagcgca tcatgtaata gatgaggaaa cggaactgat
    62881 tgttttcaat tgtgctgatt atgccaataa ccctgagctt ttgatgagtc atttatttgg
    62941 gtacgcaaaa ggtgctttta caggcgcaga tgaagaaaaa atggggatta tcgatcaagc
    63001 ggatggcggt atgctgtttt tggatgagat tcaccggttg ccgcctgaag gtcaagaaat
    63061 gatcttttac tttatggatc atggaactta cagtcgtctt ggggaaacaa cgaaatccca
    63121 tgaagcaaat gtacggatcg taggtgcgac cacagaagac ccaggttctt ctctattaga
    63181 aacttttgtt cggcgtatcc caatcaatat caaacttccg gcttttgaaa aacgtcctgc
    63241 aaatgagaaa gtcgatttag taaaaatcat gattgcccac gaagcaaacc gcacgcagcg
    63301 aaagatttct ttgacagaag atgtagcaaa agcattgatc ggcagtgtga cctatggaaa
    63361 tataggacaa ttgaagtcaa atatccagtt agtatgtgca cgaggatttc taaatcatat
    63421 gcaatcaccc gaaatctcga tcacaatcga tgatttgaca gaagggattc gtagcggatt
    63481 gattcaatta gcaagcaacc gcagtgcaat ggcagagctt tctaaaatat tggagccaaa
    63541 gataactgta gcaccaaatg atacgatcat gaaaatccaa tcggactcct atgagttacc
    63601 ttataatcta tatgatatca tcggagataa agcagcttta ctgaagtcgg atggtttgga
    63661 tcaagaagca atcaatcatt tcatttctac agatatcaac atccatctga agtctttcta
    63721 taaagaccat ggtttttctt ttaatgcaga cagcaaatta gcagaatttg tagatccgaa
    63781 aatcatagaa gtgaccaatc agatttatgt aatggtcaaa agatctttac catatgagtt
    63841 tcagcaaaac tttatttatg ctatgagctt gcatatcagt tcttttttga aaaaaatcaa
    63901 cttaggagaa gaacggcata cgaatgataa catccgtgag atggcttttg attttccaga
    63961 tgaatatgct gttgcgaaag aagtgcgtag ttatatcgaa acgtattttc aagtggagat
    64021 tccagaaagt gaagactatt acttgaccgt tctactcgtc tcgttacggg caaatcaagc
    64081 aagcggcaga atcggtgtag tcgttgcagc tcacggaaac agcacagcaa gcagtatggt
    64141 ccaagttgtc caacagttgt tagatgcaga gaacgtacga gcagtagaca tgcctttgga
    64201 tatggatccg aaaacagcat tggaccgaat tgaacgcaat gtacaagaag tagatgaagg
    64261 aagcggggtc atactcttag tagatatggg gtcccttgcg tcgtttaatt cgcaaattca
    64321 aagagacact ggaattccag tacgtactgt tgatatggta acaacttctc tagttttgga
    64381 aactgtacga aaggtctcag tattagggac agatctagat atgttgcatg aatcattgaa
    64441 aaattttcga ggttacgcag aaatatcttc tgtagaatct gttcaggaag caaaacctat
    64501 tcataagaaa aaagctgttt tggctatctg cgcatctggc gaaggtacag cacaaagaat
    64561 caaagaattg atcgaacgag cggtaaacaa gcgtcaagaa acagaattag aagttctagc
    64621 tttgtcgatt gtggaagtga aagaagaact gccagttatc caaaaagaat atcagatcat
    64681 agctactact gggattactg atttgaatat ccaagcccca tttattcctt tagaacgttt
    64741 tattgatcaa aatatcgaag tcattttgga tcagttgctg atggaatcag aattggagga
    64801 aactgaattc atctccctag atgaagagtc agctaaaaat acgtgtgttg agtttatctc
    64861 tgataacttc atctttatta atggtagtaa gttaatcgat ccaatgtggc aattttctac
    64921 acaaatctct cagacgacgg gaatcggcga tgaagaatat ggattcaaaa tcaacctagt
    64981 gatgcacaca gcaggaatga tcgaacgtat tattcggaat gaaccattga ctgtggaaga
    65041 aaacgaattg acaaatacaa cgaatgatcc tctttatagc caattggcag cctcagttgt
    65101 tctgttggaa gatcagataa aagtaaaagt gccgatagaa gaaatgtatt atctgctacg
    65161 tctagtccat aatcagttag ataaaaaaga atacactgtg ccatgaatac acaaaaacga
    65221 tacactttta tagtgtatcg tttttgtgta ttggttaaga atcctttaaa ttcaattagt
    65281 gtatcgtaaa aaagttggca cgctttttgc ttatagtagt gtgtgctagg aggtggttgt
    65341 tcacacagaa atgatagggg tttctgtgaa gtagtgagga agaaggagtt ttgcagatgg
    65401 atattcgatt ggcacgtata gatgatcgtt taatccacgg gcaagtcgca acagtttggt
    65461 cgaagcaaac aggtgttgaa cgtatcattg tgatcagcga tgtagtcgct caagataaat
    65521 tgagaaagtt tctactaaaa gaagctgcac cgccggggat cacagcaaat gtaataaccg
    65581 tgcagaaaat gattcaaatc tatcatagtg gattattatc acgagcaaaa gtgatgcttt
    65641 tattcaccaa tccccaagat gttgaagcgg ttgtccgctg cggtgtacag ttgaagtcgt
    65701 tgaatatcgg cggtatgagt tttactgaag gcaaatcaat gattaccaat tttgtttcag
    65761 taaatcaaca agatattgat tcctttcgtt acttgaacag tcaagaagtg gagttagaga
    65821 ttcgaaaagt acctgctgac aggaaaatcc ttttgatgga actactagcg aaaatcaaaa
    65881 aggtgtagca agaatggttg ttggaaaagg tcaaataatt ctgttgtaat ggttagcaat
    65941 tattaatatt tttgaatttg ttacaacaaa agtaaatagg aggtataaat atggtaggga
    66001 ttatcctagc aagtcatgga caatttgctg aaggaatctt acaatccggt tcaatgatct
    66061 ttggcgaaca agaaaacgtc aaagccgtga ttttgaaacc aagtgaaggt ccagatgact
    66121 tgagagccaa attggaagaa gcggttgctt cttttgataa tcaagacgaa gtgttattct
    66181 tagttgattt atggggcgga acaccgttca atcaatcaaa cacattattc gaagagcaca
    66241 aagataaatg ggcaatcgtc agcggtttga atttaccaat gttgattgaa gcctacgcat
    66301 cacgcttttc aatggaatct gctcatgaaa ttgcagcaca catcattgaa acagcaaaag
    66361 acggtgtaaa agtaaaaccg gaagaattag aaccggcaga agcaccgaaa gcagcagttg
    66421 aagacgcaca acctaaagga gcacttccag aaggaactgt ggtcggagac ggaaaaatca
    66481 aatatgtatt agcacgtgtt gactcacgtc tgttacatgg acaagttgcg acagcttgga
    66541 caaaagcagt gcaaccaaac cgtattatcg ttgtttcaga tgcagtatca aaggatgatc
    66601 tgcgtaagag actaatcgaa caagctgctc ctccaggtgt aaaagcaaat gttattccta
    66661 ttagcaaaat gatcgaagta gcaaaagacc ctcgttttgg aaataccaaa gcattgcttt
    66721 tatttgaaaa tcctgaagac gtattgacag ctgttgaagg tggcgtagac atcaaagaat
    66781 taaatgttgg gtcaatggcg cattcggtcg gtaaagtcgt tgtaagtaaa gtattgtcta
    66841 tgggacaaga agacgttgaa gcgtttgaga agcttgaaca aaaaggtgtg aaattcgatg
    66901 tacgtaaagt acctaatgat tcacgcgata acatggatga tattttgaaa aaagcaaaag
    66961 ctgaattagc aaaagcgtaa taaataatat taaaatttat aggaggctta tcatgtctat
    67021 tatttcaata attttagtcg tattagttgc ctttctagct ggtatggaag gtattcttga
    67081 tgaatttcaa tttcatcaac cactagtggc atgtacattg atcggtttag ttaccggtaa
    67141 cctcgaagct ggtatcgtac ttggcggtac gcttcagatg atcgctttag gttgggcaaa
    67201 tatcggtgcc gctgtagcac cagatgctgc attagcatca gtagcatcag caattatttt
    67261 agttttaggt ggacaaggtg ttaaaggtgt tccttctgcg atcgcgattg ccgttccttt
    67321 ggctgttgca gggttgttct taacaatgat cgtacgtaca atcgctgttc caatcgttca
    67381 tttgatggat gcggctgctg aagaaggaaa catccgtaag gttgaaatgt ggcacatcat
    67441 tgctgtatgt ttacaagggg ttcgtatcgc ggtcccagca gcagcgttgc tatttatccc
    67501 agcacaaact gtgcaatcat tccttgaatc aatgccagca tggttaacag atggtatggc
    67561 tatcggtggg ggaatggtcg tagctgtagg ttacgctttg gtaatcaaca tgatggctac
    67621 aaaagaagta tggccattct tcgttattgg tttcgttgta gctgcaattt ctcaattaac
    67681 attgattgct ctaggtgctt taggtgttgc attagctctt atttacttga acctttctaa
    67741 aatgggtggt tcatcaaata atggaggcgg aagcaactct ggtgaccctt taggcgacat
    67801 tttgaatgac tattaatctt gaaggaggaa aataaaatgg cagaagaaaa aatcaaatta
    67861 tcaaaaaaag atcgtttagc tgttgcgtgg cgctctacct tcattcaagg ttcatggaac
    67921 tacgaacgta tgcaaaacgg tggctgggca ttttctatga ttccagcaat caaaaaatta
    67981 tacaaaacaa aagaagaacg ttctgcagca ttaaaacgtc acttggaatt ctttaataca
    68041 cacccataca ttgcttctcc aattttaggg gttacattgg ctctggaaga agaacgtgcg
    68101 aatggcgcgc ctgttgatga tgtagcgatt caaggggtta aagtaggtat gatgggtcct
    68161 ttagctggtg ttggtgaccc agtgttctgg tttacagtac gtccaatgct aggtgcacta
    68221 ggtgcttcat tagctatggg aggaaatatc cttggaccaa tcatcttctt cttaggttgg
    68281 aacattattc gttgggcatt catgtggtat acacaagaat ttggctacaa agctggttct
    68341 aaaattactg atgacctttc tggcggattg ttacaagacg taacaaaagg tgcttcaatt
    68401 ttagggatgt tcgtattagc tgcccttgta caaagatggg tatcaatcaa attctcacca
    68461 gttgtatcaa ctgtaaaatt agataaaggt gcttacatcg aatgggataa attgccttca
    68521 ggtggtgaag gaatcaagag tgcatttgaa caagttaata atggcttagc cctttctcct
    68581 gaaaaggtaa caacattaca agacaactta gaccaattga ttcctggttt agctgcatta
    68641 ggattaacat tgttatgtat gtggttgctt aagaaaaagg tttctccaat cgttatcatc
    68701 cttggtttat tcgtagtcgg tgttgttgga cacgtcatcg gacttttata atccataaaa
    68761 atatagctcg ggcgttgtcc cgagcttttt ttatatagta tactgttcat aaacaaatat
    68821 ctggagggag aaaatggttc aatcaatcaa tacaaaagta gacttggtaa ttgatgctac
    68881 ttcgtttacg ggtctgacag attacgggca aatcatgata ggtgataatg gttttgagtt
    68941 ttaccatgcg cgagatagac gcaaatatat ccaaattcca tgggaagaag tggattatgt
    69001 actagcatca gtcatgctaa agggaaaatg gattcctcga tatgccattc aaacgaaaaa
    69061 aaatggtaca tacacatttt cttccaaaga acctaaaaaa gtgcttcgtg cgatcagaga
    69121 atatgtagat gaagatagaa tggtacaatc attgagtttc tttgatgtag tgaaacgtgg
    69181 tattcgtagt atgtttaaac gcaagtaaaa aataaagaaa acttctaaga ggaggttttc
    69241 tttatttttt tactttgcag aagaaaatat gaaatagcaa aaccaattat ttgtcccctt
    69301 taaatcccat atgctaaaat tgattagcaa agaaggaggt tatattatga gttggttatg
    69361 gacattgatt gttggagccg tgatcggagc aattgcaggt gcgatcacca gtaaaggaaa
    69421 ttcgatgggc tgtgtaacga atatcattgc tggactagtc ggttcaagta ttggacaagc
    69481 gattttcggc gaatggggac caaaagctgc aggtatggca cttattccat ctattttagg
    69541 cgcagtgatt ttggtagcag ttgtctcatt tttctttgga aaacgatcat aagtttttac
    69601 aaaatagtaa actaaaagga gttgcagcga gactgtgact cctttttggc tttatttatt
    69661 taaaataaaa agaagttttc cggaaatttt tatttaactg tcgtgtacga agaaaagttt
    69721 gctataatgg aaaaaagcgt gcaaagaaag agggagtttc attatgatgc tacgtgtaga
    69781 aaaattgcgc aaaaaaatgc aagaagaaaa tttggattca tttttggtta ccagtccgta
    69841 caatcttcgt tacttaacga actttacagg tacgacagga ttagctgtta ttactttgga
    69901 aaaagcattt tttattactg attttcgtta taccgagcag gcggcagcgc aggctcaagg
    69961 gtttgaaatt attaaaaatg tcggacctat ttttgatgag gtagcaaact tagtacgcaa
    70021 agaagaactg cactcattag cttttgagga aacaacagtc tcatttttag aatattcggt
    70081 tttagaagaa atcatcgatg cggaattagt acctgtttca cagatgatcg aagaactcag
    70141 agaagtgaaa gatgaagaag aaatcgctat tatcgaaaaa gcatgtcata ttgcggatat
    70201 ggcatatgat cacatcctga aaatgatcca accgggcatg actgagattg aagtcgcaaa
    70261 tcaactagac ttttatatgc gttctcttgg cgcttcaggt gtttcttttg atacgatcgt
    70321 tgctagcgga ttgcgttcgg ctatgccgca tggtgtagca agcaaaaaaa tcatcgaaca
    70381 aggtgacttg atcacattgg actttggttg ctattacgaa gggtacgttt ccgatatgac
    70441 acgtacattc gctattggtg atcctgggga aaaattaaaa gaaatttatc aaatagtctt
    70501 agatgctcaa ttagcagtta ttgatgctgc aaaaccagga atgactggtg tccaattaga
    70561 tgcagttgct agagattata tttcaaaaca tggttacggg gaagcatttg gacattctac
    70621 aggacatggg atcggtttag agatccacga aggaccaaac gtttcttctc gtgcagaaaa
    70681 gcaatttgta ccaggaaatg tgatcactga tgaaccaggg atctacttac caggaatcgg
    70741 tggagtacgt attgaggatg acttgttgat cacagaagat ggaaatcgtg tgttgactcg
    70801 ttcaccaaaa gaattgatca tcttatagtt tcatcaatag aagaagtaag cgtaaaaatc
    70861 agtgttcaca gactttgtta tagaaaatca ctcgatttaa tgatacacta tagaagaact
    70921 tttaagctgt aagagataat aaagttgggt gttttctcca attcatagca gtttgcagct
    70981 gatcggcggc agttttctta ccacgtgatc actatgccgc atcatagaga attcggatgt
    71041 atctcgtgct taggcaaagg gaggaataaa taaatggcag atgaaaaaaa tttgatttta
    71101 aacgcaaacc aagatttagg agagatcgtg atcgctcctg aagtcatcga agtaattatc
    71161 gggattgcag cttctaaagt agaaggcgta tacggaatgc gcggaacttt tgccagcaat
    71221 gtcactgaat tattaggacg tgcagctcac ggaaaaggtg tttacttagc aaacgaggaa
    71281 gatggcttga aagtagatct ttactgttat ctagaatatg gtgtttcagt tcctaaagta
    71341 gcaatggaaa tgcaggaacg ggtcagccag caagtccttt tcatgacgga tatcgattta
    71401 tctgaagtca atatccatgt agtagctgtt gttcctgaaa aattagaaca accaacaatc
    71461 gaagaacttt ttgaagacga agaggaagag aatgaataaa gaattatcaa gacacgagat
    71521 tcgggaaatg gcattgcagg ctttatttcc attagacttc aatgcagacc taacaaaaga
    71581 agatgcgatt ttcaacgcaa tcgaattaga ccaccgcgac atgatcaacg aagatgaatc
    71641 agagtttgtt cctgtttatc ttgatacgct agttggcggt gtctgtgcaa aaaaagatga
    71701 attggataaa gtgattgaaa aacatttaaa aaacaattgg cacatcaatc ggatctcaaa
    71761 aatggatttg gtgatcttgc gtatcgcgat ttttgaaatg acttatgtga cagatgtacc
    71821 agcggctgtt gctttaaatg aagcaatcga gttggcaaaa acatttagtg atgaccgttc
    71881 acgtaaattc gtcaacggtg ttttatccaa tgtattgaaa gaattagaag ctggggcgta
    71941 agtcctagct tttttctatg aatcatcata aacctgttat aaatcgggaa aattctatgg
    72001 tagaatgaaa acacaaaaaa tatctgggag tggttagatg gcagaattga ttaacggaaa
    72061 agaattagct gaaaaaatgc aggcagagat tgctgaaaaa atccaaaaat tgaaagataa
    72121 tggtatccat cccggattag tcgttttatt ggtaggggaa aatccagcca gccaaatcta
    72181 tgtaagaaat aaagaacgtg tagcaaaaga aatcggaatc cattcattgg tagagcgtta
    72241 tcctgagacg atttctgaag acgaactact tgcagaaatt gaaaaataca atcaagatcc
    72301 tagttttcat ggtatattgg tccaattacc attgccaaag catatagatg aagaaaagat
    72361 cctgttagct atccgccctg aaaaagacgt agacggtttt catccgatga atttaggcag
    72421 actccttgcg ggtaaaccgg acaagattcc ttgtactcca tacggcatca tgaaaatgtt
    72481 tgaagcttat tctatcgatc cggcgggtaa acgagcagta gttatcggac gaagcaatat
    72541 cgtcggtaaa ccgatggctc agctactctt gatggcagat gcaactgtta ctgttgctca
    72601 ttctaaaacc gaaaatctgg ctgaactggc acaagaagca gatattttag tcgtagcaat
    72661 aggacgtgga cattttgtca caaaagaatt cgtaaaacca ggtgctgtag tgatagatgt
    72721 agggatgaat cgtgatgcta acggtaagct gatcggtgat gtgaaatttg atgaagtgga
    72781 gcctattgca ggctggatca ctcctgtgcc aaagggtgta gggccaatga ctatcacgat
    72841 gctgatgtat caaaccgtaa aatctgccga agcttttgga gcaggtgaat agatggcaca
    72901 agaatattta actgtcacta cattaacgaa gtatttgaaa agaaaatttg atgccgatcc
    72961 ttatttagaa cgcgtatatt taacaggcga aatatcgaat ttccgtttac gtgccaatgc
    73021 ccatcaatat ttcagcttaa aagatgacca tgcaaaaata tctgctatta tgttcaaagg
    73081 agcattccaa aaattacgat tccagccaaa agaaggaatg aaagtccttg tcgttgggcg
    73141 tatttcatta tatgaagcgg gaggttctta tcaaatctat gtagagcaca tggagcctga
    73201 tggaataggt gctttatatc aagaattgga agaacgaaaa gaaaagctga tgaaggaagg
    73261 cctttttcaa gggcctaaaa aaatgctgcc acgtttccca aagcgaatcg cggtccttac
    73321 cagtccaagc ggagcggtta tccgcgatat catcacgacg gtgaaaagaa gatatccaat
    73381 tgtccaattg gttttgttcc caacagttgt acaaggaaac caagcagcgg acaatgttgt
    73441 caggaatatc cagagagtag aagagatggg caatttcgat acaatgatca tcggtcgtgg
    73501 gggtggttcg attgaggatt tatggccgtt taatgaagag cgggttgctc gggcgattta
    73561 tcaagctaat acgccggtga tttcatctgt aggacatgaa acagatacca cgatcgctga
    73621 tttagtagca gatgttcggg ctgccactcc aacagcagct gcagaattag ctgttcctgt
    73681 tttgtcagaa gagttgatgc gcattcaaga acggcaagct agattgcagc aggcgtttgt
    73741 acgccagatc cagcgcaaac aagaacggtt cgagcgtgct cgacgttcct atgtatttcg
    73801 gcagcctgaa agattgtatg aaggtcagac catcaaatta gaccagttga aacagcgatt
    73861 atatcaagct acacagcaaa tttatcatgc gaaagaaaaa caaacgatcg cattaagcca
    73921 ccaattggaa caagtggctc cgctttatcg agttaaagca gctcgccaag aaactgctta
    73981 tctagaaaaa cgcttagaag agaaaatcca acaatatatg aaacaacagc agcagcgttt
    74041 tgctcaagct gttcaatctc ttgatttgtt gagtccattg aaaatcatgg gaagaggcta
    74101 cagctatacg acgttaaatg atcaagtagt aaaaagtgtc accgagattc atcaagggga
    74161 tttgctgacg atccattatc aagatggaat cgttaaagga caagtacaag agatagacaa
    74221 aggagaataa aaatatggaa aatccaactt ttgaagagtc actgcaagaa ttagaaaaaa
    74281 ttgtcatgca gttagaacaa ggagacgttc cattagaatc agctctagat tcgtttaaac
    74341 gaggaatgga attaagcaaa cattgccaag atacattagc taaagcagaa gagaccttga
    74401 cgaaaatgat gacagaatca aatgaagaag ttgattttga cggaaacgag gaaagctgag
    74461 atgagattaa ctgacttttc agctattcat ctacctgctg tagaaaaaga aatcttgtct
    74521 tttttagatg agcatacaac ggatagagga ctctatgatg cgatgagtta ttcagtgaaa
    74581 gcaggcggga aaagaatacg tcccttatta cttttaacgg ccgttgcttc ctttgatgag
    74641 cctattgatg ttcccgtcta tcaagtagca gctgctttag aaatggtgca tacgtattcc
    74701 ttgatccacg atgatttgcc tgcgatggac aatgatgatc ttcgtagagg aaaacctacc
    74761 aatcataaag tattcggcga agcattagct atattggccg gagatggttt gcttacagga
    74821 gcattccagc taatcagtat ggcacactta ggtaattctc ctaagcttct gctgcttcag
    74881 caattagctg tttgtgcggg atcacaaggt atggttgctg ggcaagctgc agatatcgag
    74941 ggcgaatcga aaaagctttc tttagaagaa ttagcattta ttcatgaaag aaagacaggg
    75001 catttgattc gttatgcctt gctggctggt ggcatattag cgaagcaacc tgaagaaatc
    75061 ttattgctac ttcaacgatt agccgaacat ttaggtttag cttttcagat aagagatgat
    75121 ctactcgatg tgattggtac aacgaaaaca ttagggaaaa cagctggtaa agatgaacgt
    75181 atggaaaaaa atacttaccc gcgtttatta ggcctagaaa agacacgtga ggcgttggag
    75241 attgagctgt tatccgccaa caagatcata gacaaattag aagaggacgt tttttctttt
    75301 gatggcgggc tgatgcgcca gatgatcaag caatttgatg tggagaaatg agggaaagaa
    75361 tggaaaaaga acgcgtagat gtattagcgg tcaaacaagg attatttgaa acaagagaac
    75421 aagccaaacg ttcagtgatg gctggtttga tttacaacga aaaaaacgag cggtttgata
    75481 aaccgggaga aaaaattcca gtgagtagtg aattaagagt caaaggtaaa aaacttcctt
    75541 atgtttctcg agggggatta aaattagaaa aagcactcaa agcatttgat ctatctgttg
    75601 aaggaaaaac attgttagat attggtgcat ccactggcgg atttactgat gcagctttgc
    75661 aaaatggcgc aaaaatgagt tatgctttag acgttggcta caatcagcta gcttggaaaa
    75721 taagacaaga tcctagagtc gtcgtaatgg aaagagtgaa ttttcgttat gcaaaacccg
    75781 aagactttac cttaggaatt ccagaagttg ctgttatcga tgtttctttt atttcactaa
    75841 aactgatgct gcctccatta catgctattt taaaagaaga gggagaagtg attgctttaa
    75901 tcaaacccca attcgaagca ggcagagaag ctgtagggaa aaacggaatt gttcgtgacc
    75961 ctcagaccca tcaaaaagtt ttagaagaca ttttgtcatt tgcggcacaa ggtggatatg
    76021 atgtgctgga attaagttat tctccgatca cggggggaga aggaaatatc gagttcttgg
    76081 ctcacttgag gaaagtaccc gaaagcggaa caatcaattc agctattaat atggcagaag
    76141 tggtttcaaa tgcacacgaa cagtttgacc ataaataaat tgtaactgtg aaaatcaagt
    76201 gagaagtatg cggattcttc tttattatac caaggaaata agctatcatt gattaagaag
    76261 aatatgctaa ggagcgaaga cgtaatgaga aaaaaagatc ggcatcgttt gattactcga
    76321 ttgttgagtg agcaagatat ccgaaagcag gaagaatttg ttgaaatcct gaaaagcaaa
    76381 ggaatatctg ttactcaagc tacgatttcc agagatataa aggaactgaa actgatcaag
    76441 gtaccggctt cagatggcgg ctaccgttat agcataccag ctgaaacaaa cgaagatgta
    76501 aatactaaat tggaaaaact attaaaagac gctttcgtgg cagttgacca aatggaaaaa
    76561 tttgttattt tgaaaacatt gccgggaaat gcttcggcag cagcaaattt gattgataaa
    76621 cgttacaaga aagaattatt ttcgattatc aatgatgacg ataatgtttt gatgattaca
    76681 cgaactgaag accaggcagt tttattgaag aaagacttcc tgcattacct ttaagttgag
    76741 gagcgaatgt gatgctacaa gaaatcagta ttactaactt tgcgattatt ccggaattgc
    76801 ggctttcttt tcacgaagga atgaccgcgc tgaccggaga gactggtgcg ggtaaatcca
    76861 ttattatcga tgctttaggt ctgttagcag gaggaagagg ttcaagcgat tatatacgtc
    76921 aaggagcaga aaaatgtgtg cttgaagggt tgtttgaact accaaaacaa gaaggattct
    76981 ctgaactgat ggttgaatta gggatcgaaa cagatgagga caatctgatt gttcgacggg
    77041 atatgtcctt aactggaaaa aatgtttgtc gagtgaatgg tcatattatc acattagcta
    77101 atctgagaag aatcggcagt tatttagtag atatccaagg acaaaacgaa catcaggaac
    77161 tgctccagcc tgaatcacat cttgctttat tggatcgttt tggagatgta gctttccaac
    77221 agaagaaaaa gagttatcag caagaatata tctcttatcg tgagctggaa aaaagggtac
    77281 gaaaagtgca gcaaaatgaa aaatcctatg ttcagagaat agatatgctc catttccagc
    77341 aagaagaaat agctgctgct gatttgcaag tcggagaaga agagaaatta aaagaagaaa
    77401 gagaaaaact tagcaattat caaaaaattg ctgatggatt agctgcaggt tacggagcac
    77461 tgacgaataa tgaacaaaac agtgtagatg gtgttggctt ggccgtttca gaactgcaag
    77521 ggatcgccca tctagatgtg gagtatgaag caatttatga aaatatccaa agtgcttact
    77581 atttgctgca agatgcaatc ggcgatatga gtcgtcagat tgatttattg gagttagatg
    77641 agagtcgttt agaagaagtt acgcaacgtt tagaattaat ccgacagttg aaacgtaaat
    77701 acggcgaatc cattgagtca atattggctt actatgacga aatcaccgaa gagctagctt
    77761 cttctgattt ttcagaaggt caattggaca aaatgaaaga ggaactagag caaaaagagc
    77821 ttctacttca gcaacaagcc gcagatcttc atgaagcgcg taaaaaaatc gcaaaagaat
    77881 tggaaaaatc cattctgcat gaattgaaaa gtctgtatat ggaaaacact gagtttgagg
    77941 tacgtttctt aaaagaagaa aatagacaat tgaatcatga tggatttgac caaatcgaat
    78001 tttacattac gaccaatcca ggagagccac tgaagccttt ggtgaaagtc gcttctggtg
    78061 gcgaactgtc tcggatgttg cttgctttga aaactatttt ttcttctgag caaggtgtga
    78121 caagtattat ttttgatgaa gtggatacag gtgtgagcgg aagagttgca caggccatcg
    78181 cagataaaat ttctcaaata tcgaaatact ctcaagtttt gtgcatcact catctgcctc
    78241 aagttgctgc agtcgccgac tatcaatatt atattgtgaa agcagtgatt gatggacgta
    78301 cgcaaacttc tgtttcagag ttgaaaacaa aagaaagaga agaagaaatt gcacgtatgc
    78361 tggctggaag tgagattaca aaactcacac tcgaacatgc aaaagaacta ttgaaactcg
    78421 caaaaaggac ctgagccacc tcgcagtggt tcaggtcctt ttcagcagct gatagctata
    78481 ttttattttt acatgacact cattacagca gtcaatacaa cagatccaat cgttaaaatc
    78541 agcattgtcc aaatgacaaa ttttgttact ttagcaaaag tacttgtttg ttttttttca
    78601 cgcattgttt tcaacctcgt cattaattag ttgattcaag tgtacaatct tttttccatt
    78661 tccacaagag ggctttttta taaaaacttc ccttttttta aataataggt agttacgata
    78721 caaataaata atgttaatag aactagccat ataaaggcat catccctttc agcaataggt
    78781 aacttaatgt tcattccata gatactgccg acgattgtcg gtatagttaa aataatcgtt
    78841 aaagaagtta aaattttcat aatattattc agattattcg atacgattgc tgaaaaagtc
    78901 tgattcattt gttctactag ttttaattga atgcgagaag tagtcattgc ctgctctgtt
    78961 tccaccaata catcacgtaa atgatttttc aaagcatgat tttcttggaa aagtttattt
    79021 tgcgctagtt ctttcaatgc atctaaatta gcagaagtcg cttctctgaa ataaacgagg
    79081 cttttttgga tatccatgat ttgataaagt tgtttgtttt cagtggatac ttgaagtttg
    79141 gcttccaaag tatcgcattg agattgaagc gtagctaaat aactgttgta agagagtgtg
    79201 acctgccaaa tgaattgaaa gaagatattg agattaggcg aaatatcact ttttggaaag
    79261 gtctggctaa agactttctt taaaaacagt ggttcatgat tcgtaattgt gataagcttc
    79321 tttttgggcg tcaaaataat agagagcggg tatgtatcca cttgaaaata accgctagga
    79381 cttgttttag caaaaggata ttgtaacaat aataacgctg gctttgtcaa ctttgattga
    79441 ttgaattctt ctgtacggga attttctgca tcgtccaata tggaagtgat gtaatctgac
    79501 ggaatttcaa attgatgtgt cagctgatga atctcttctt gattaggacg ttctacacaa
    79561 taccagatcg tttggtcagt ttcctttccg gtattacgca gtttctcatt atctaatgta
    79621 aaatatttga tcatgatttt cctcctgttg cctgtttctt ttattataac ggatagggaa
    79681 ttggaataca tggaatgtgt ttgtaagata tagaatataa ctgcataaat ttaaataaaa
    79741 tgaaacagtt gtgtaaagta tatacagtat acttgaagca acgataaaaa aggagtttac
    79801 gttcatgggc acattcacac cacttatctt gttttggtat atttacccgg taatcgtttt
    79861 gtttggttgc caatttcttg taaaattatt ttcgttaaat cgtcgtttta aaataaaagc
    79921 ccctgatttg gcgattcctt ttttgtgggg cggtatgcat gcattgtcta gaaatacagg
    79981 gacaatttct atcttaccgt ttttctttat ttctgttttg ctaatgggaa tttgtattgc
    80041 tgttttccaa gcatattatt atgaagaaat tatttatccg cgttatttca agatgacttg
    80101 gcgtttggtt tttctgttga caatcgtctt gtacgctgtc ttgatcattg tgaacatact
    80161 ttcttaccta tgaaaaaaat agaaaagctg taaaaagtct tttaaaaggc tttttgcagc
    80221 ttgattttta aaaaaatgtt tatgaataaa aaaagttgtt cccggatttc ccagaaaagg
    80281 tggtagaaag tgggggattg tggtagacta attttagtta gtggaggaat ggggggcttt
    80341 caatgttgat gggtgaattc caacataata ttgatgcaaa aggacgttta atcgtgccct
    80401 caaaacttcg tgaagaactt ggcgaaaagt ttgttctgac acgcggattg gatggctgtc
    80461 ttttcggcta tccgatgagc gaatgggaaa atctggaagc taaactgaac gaaatgccgt
    80521 tggctaaaaa agacgcacgt acttttgtgc gattcttcta ttcggctgcc actgaatgcg
    80581 aacttgataa acaaggacga atcaatatac ccagcacgtt aagaaattat gcagctttga
    80641 caaaaggttg cgtagtcatc ggtgtatcta accgaattga aatttgggat gagacacgat
    80701 ggcaagattt ttctgcagca gcagaagaga actttgacga aattgctgaa agtatgattg
    80761 atttcggact ataagaatag gaagaggctg actaatgact gaaacatttc aacactatac
    80821 agtcatgttg aaagaaacgg tagatggatt gaacatcaaa ccggatggcg tttatgtaga
    80881 ctgtacactc ggcggtgccg gacatagtga atatttatta agccaactaa gttctaaagg
    80941 gcatctgtat gcgtttgacc aagatcaaaa agcaatcgat cacgccaagc tgcgtttagc
    81001 ttcttatgtc gaacaaggta tggtcacatt tatcaaagcg aacttcagag aattagaaga
    81061 acgcttgagt gagcatgtag ataaggtaga cggcattttg tatgatctag gggtttcctc
    81121 tcctcagcta gatgaagcag aacgtggatt tagttatcat caagatgcac cgttggatat
    81181 gcgcatggat cagtcagctc ctttatccgc ttatcacgta attaatgaat attcctacca
    81241 tgaattagta aagattttct tccgctatgg cgaggagaaa ttttccaaac aaattgctcg
    81301 tgaaatcgaa cgagtacgaa aaacaaaacc aatccaaaca actggtgagc ttgtcgaaat
    81361 cattaaacaa gtcatcccag ctcccgcaag aagaaaaggc gggcatcctg caaaaagaat
    81421 ttttcaagct gtgcggattg cagtaaatga cgaactcggt gccgaagagg cctctttaga
    81481 acaggcaatc cggttattga aagtcaatgg aagaatcagc gtaatcacat tccattcatt
    81541 ggaagacaga attgtcaaaa gtatgtttaa agaatatagt acggtacaag atctaccacc
    81601 gggacttcct gtcatacctg acgaattcca accagaactg aaagtcatta ctcgtaaacc
    81661 aatccttcct tcagaggaag agctaacaga aaacaatcga tcacgcagtg caaagttacg
    81721 tattgcagaa aaaataagaa tgaaagagga gtagaatgat ggcagaatta aagaaaatgc
    81781 aagattacga atatgattta cctgaattgg tggatcagcc ggaacaacaa atgcctgatt
    81841 cttcttcgaa tcaagaaatc attcttccac aatcaccggc aaaacgatta aagcagattt
    81901 ctgctttgga aaaaattgct gtggcaagcg tggcgattgc tattgttgct ttatgtgttt
    81961 taacagttat gctgcgcaca aatatcagcg gagctgaaaa agaaatcacg tcaatacaaa
    82021 cagaaatcaa tgataaaaag caagaaaaaa cgagtttaga acaagaaaaa aacgaacttt
    82081 ctcgtactga acggattaaa caaatcgcag aagaaaaagg attgtctatt aatgacgaca
    82141 atttaaggaa agtgaagtaa atgagtctta aaaataaaat tcggcggttt atggaaaaga
    82201 aaaatctaaa tccgatgaac aatcgcaaaa aagtaggtat cattttattt gctacgagta
    82261 ttggattgtt ctttttattt gccgtccgtt tctcgtatat cgttattgga ggtcatgttg
    82321 ctggtacctc attagctgaa aaaacaaaac agttgtacca aggaagtgaa gtagtaaaag
    82381 caaaaagagg aacgatttat gatcgtaacg gtgtggcgct cgcagaagat gcctcctctt
    82441 attcaattaa agcgatactc tcaaaaacat atacctctgg ggataaaaaa ctgtatgttg
    82501 aagaaaagaa ttttgataaa atagcagaaa tcctgcataa gaatctttct atagacaaaa
    82561 aagatgcact gaatatttta gaggacggcg caaaaaaaga gctgtatcaa gttgaatttg
    82621 gtagctatgg gaaaaatatc tcccaagaaa ccaagcaaaa catcgaagcg gacatgaaaa
    82681 aagaaggtgt cgctggtctt tattttgtcg accatcaggc tcggatgtat ccaaatggcg
    82741 tcttttcttc tcattttatt ggatacgcag ttccagacaa agatgagaat ggcttagttg
    82801 gtaaactagg tttggagtct gcttacaatg atatcttgag cggtaaagat ggtaagatta
    82861 tttatcaaaa agataatttt caaaatcctc tgccaggtac agtggcagaa gaagaaaaag
    82921 ctgtcgacgg acaagatatc tacacgactt tagacagcag actacaaagt tatttagaaa
    82981 cactgatgga tcaagtaaat gaagaatatc agccagaaga attgacagca gtcctgatga
    83041 aagcaaaaac cggagaaatc ttggcaatgg gacaacgacc tactttcaat cctgaaacaa
    83101 tggaaggatt gactggaaaa gatgctatct ggcgtaattt cttggtacaa gatagttatg
    83161 aaccaggatc aaccatgaaa gttttcacta ctgcagctgc tatcgaagaa ggagaattca
    83221 acgagaatga aaccttccaa tctggaaaaa tccaagtaga agatgctacg atcaatgacc
    83281 atgactttgg tgaaaaaggt gtgctgacca tgcgtcaagc tctttcatgg tcaagtaacg
    83341 tcgggatggt catactggaa cagcgattag gcggacgatg gtataactat ttacaaaaac
    83401 tgggatttgg acaaagtact cattctggat tagatgatga agtaaacggt gctttaccta
    83461 cattaaatat tgtcgaccga gcaatgagtg cttatggaca agcggtaggt gtgacgaatt
    83521 tccaaatgat gaaagctttc acttctattg cgaataacgg tacaatgatt caacctcgat
    83581 atatcagcaa agtggtggat ccccaaactg gagaagagcg aacaactcaa acagaagttc
    83641 tagggcaacc tttttcaaaa gaaacaacag aaaaagtccg tgaatatatg agagatgttg
    83701 tcgaaagcga aaactacggt agtgcttatg gtgtatatag tgtacctgga tacaatgtat
    83761 cggcaaaaaa cggaacagcc caaatcgctt ctgatacagg gggatatcaa acaggagata
    83821 cagcctacct gtattcgatt gtggagatgg ttccttctga agatccagac tatgtcttgt
    83881 atctaacaat gaaacaccct aagacatatg atcgaatggc attggctaaa atcgcaaatc
    83941 cattgatgaa acgggcaatg gattttaaag aaactgaaga agattcagat acagaaacaa
    84001 aaacagaaaa agtatccgta gccgattatc gtaatcttga agcggacgtt gcagcagctg
    84061 atgctcaaaa aagtggactg caacctgtag tgatcggtaa tggtaaaaaa gtacaaaaac
    84121 aatctactgc caacggtgat cagttgatct ctggagaaaa actgatccta tatactggtg
    84181 gtgataagct gatgcctgac gtaacaggat ggtcaaaagc agatatcatg aagctgggga
    84241 aaatcctcgg tattgaagtt agttttgatg gagatggtta ttgtgtcaaa caagaacttg
    84301 ctccatatga aaaaatcact gaagacaaac ttagttttac tttagaagaa taagaagaga
    84361 aataggagag aaaagagcat ggaatggaca caagcattga ttcccattgt cagcagttgt
    84421 gcaatgacaa ttgccgcaat gccgctattt attggttatt ttcaaatgaa aaagcaaggt
    84481 caggcgattc gtgaggaggg accaaagtgg cacaatagca aagccggaac tcctacaatg
    84541 ggcggcttag tgtttctgat cggctctatt ttgacaggaa tttgggttgg agcttggcaa
    84601 aaacagctaa ctcctactct tttcatcttg ctttttgtat tagcgttata cggagtaatc
    84661 ggctttcttg atgattttat aaaaatattc aagaaaagaa acatgggact gaactcgaag
    84721 cagaaattga ttggacagat tctaggggga atcatctttt atcttgttta ccgttctgaa
    84781 ggttatccag gtgtattgaa tttcttcgga ctagatctac ctttaggaat catttatggt
    84841 attttcgctc tcttctggct tgttggattt tcaaatgcag taaatctgac agatggcatc
    84901 gatggattag ttgctggctt aggaagtatc tcttttgcag cttacggtct aattgcttgg
    84961 catcagcatc agtatgatgt attaatcatt tgcttaagtg ttttaggagg tttactgggt
    85021 ttcttcgtct acaacagaaa acctgccaag atttttatgg gagatgtcgg ttcattagcg
    85081 ttgggcggac ttttagcggc aatttctata atgctaaacc aagaatggac attgcttttg
    85141 attggtttga tctatgttat ggaaacagca agtgtgatgc ttcaagttac ttcctttaag
    85201 cttacaggaa aacgaatttt taaaatgtct ccaattcatc atcactttga aatgtgcgga
    85261 tggtcggagt ggaagattga tacagttttt tggttgatca gcattgtgac ttcgttgatc
    85321 acattatgga tcgtttggta ggagatgacg ggaaatgaaa aaaatatcaa catatgaaaa
    85381 taagaaagtg cttgtcttag gactcgcaaa aagcggagta agtgcggcca aattattaca
    85441 tgaattgggc gcacttgtca ccgttaatga tggaaaacct tttgacgaaa atccggaagc
    85501 acaagagctg ctttctttag ggataaaagt aataacaggt agtcatccaa tcgaattgtt
    85561 ggatgaagaa ttttcattga tggtcaaaaa tccagggatt ccctacagtc atcctttagt
    85621 agcaaaagcc caagaaatgg gtattccggt aattacggaa gttgagctag cttacgaagt
    85681 agcggaatgc ccaattattg ggattacagg aacgaacgga aaaacaacga caacaacgat
    85741 gacaggacta ctactgaatg caggagcaga tcaaggaatt gcacgtttag cgggtaatat
    85801 tgggtatcca gcaagtggtg tagcgcaaga agcaaaatca gaagataaaa ttgtgatgga
    85861 attgtctagc ttccaactca tgggaatcac agatttccgc cctcatatag cggtgattac
    85921 caatatctat gaagcacata ttgattatca tggtacaaga aaagaatatg tcaaagctaa
    85981 atggaacctt caaaaaaaca tgacagaaaa agattatctg atccttaact ggaatcagtc
    86041 agaactacaa gaattagcgc aaagaacaaa agcacgcgta ctgcctttct cgacaaaaga
    86101 agtgttggaa gacggtgtgt atgctgacga ttactcaata tactataaaa aagaaaaaat
    86161 aatggaaata tctgaacttg gcgtaccagg taagcataat gtagaaaatg cactagctgc
    86221 tatttctgta gcaaaattgt atggtatatc aaatgaagct atacgagaaa cgttgcattt
    86281 tttccatggc gtaccacatc gtacgcaata tgtaggagaa atccaaggca gaaaatttta
    86341 taatgattca aaagcaacca atattttagc gactaaaatg gctctaagtg gatttgaaac
    86401 gtctaaggtc gtgttattgg ctggcggatt agacagagga aatacatttg atgaattaat
    86461 tccttcatta aaaggaatca aagcaatggt cgtatttggc caaacaaaag aaaagttgat
    86521 ggatgctgga aaaaaagcag gcatcgagac aatagtaaca gccgattctg tagaacaagc
    86581 agttccttta gcattagaaa acagtacaga tggtgatgta gttttattat caccagcgaa
    86641 tgccagctgg gatcaatacc caaattttga aactagagga aacagattca tggaagctgt
    86701 gaaccgatta aaatagaaaa agtagaagcg agcgataaaa aatgaaaatt ttggttacag
    86761 gcggaggaac agggggacat atttatccag ctcttgcctt tgtcaattat gtaaagactc
    86821 aagaaccgaa tgctgaattc atgtatgtgg gagcaaaacg aggtctagaa aacaaaatcg
    86881 tccctgatac aggaatgcct ttccatacct tggagattca aggatttaaa cgaaaaattt
    86941 ccatgcataa catcaagacg gtccgattgt ttcttaagag tatccgtcaa gcaaaaaaaa
    87001 tcctcagtga ttttcaacca gatatcgtta tcggtactgg aggatatgtt tccggagcgg
    87061 tagtgtacgc tgcagccaaa atgggtattc caacagttat ccatgaacaa aacagtgtac
    87121 caggtgttac caataaattt ttaagccgat atgtggatcg gatcgctctt tcatttaaag
    87181 atgcggcttc cttttttcct gaaaacaaag cagttcttgt aggtaatcct cgagcacaag
    87241 aagtagcaga tactaaaaaa tcagatattt tgaaaacatt tgatttggat ccagaaaaga
    87301 aaacagtctt gatttttggc ggtagtcaag gagcacttaa aatcaatcaa gcagtatcag
    87361 tttttttaag tgaatatatt ccttcttctt accaagtttt gtatgcttca ggagatagat
    87421 attatgaaga gatcctaaat aaaataaatc ctgaaataca aaatattagt gtccagcctt
    87481 atatcaaaaa tatggcagag gtcatggctt ccagtgatct gctggtgggg cgtgctggtg
    87541 caacttcgat cgcagaattc acagctctgg ggttaccggc agtgttggtt cccagcccat
    87601 atgttacgaa tgatcatcag actaaaaatg caatgagtct tgttcacgca ggagcagcaa
    87661 aaatgattgc agataatgaa ctaacaggtg aatcactaag tcagacagtc aacgaaatca
    87721 tgggtgatga agaattgcaa aagcaaatgt gccgagcttc taaagaacaa ggaatccctg
    87781 atgcttcaaa acgtctttat gacttagtaa aacaaataat caaaaaataa aggcggtggt
    87841 cttagtgagt aagaaaaaag aaccaaaaaa attgtctcat ccgtcttctg atgaaaacaa
    87901 tttgactcct tggcaaaagg ctaaccgaaa atatctggca gaacaagaga tgaaaaacca
    87961 agagccaaaa aaagaagaca taacattaga tacacccctt tcagattctg aagaagaaaa
    88021 aacggttcaa gaggaacagc aagaagaaag ccagtccgag aaaaaaatcg aattttttga
    88081 agagatcaag caaaagaaaa acggtggacc atataacggt tcttttttga accggctgcc
    88141 caatttaaaa agtcaacggc acaaagtact atttcgtcgg ttagctttga tcatcactgt
    88201 attgggtata ccgctgattt ttttgatcta ttatgtttct ccttatagca aattacaagc
    88261 agttgcagta tcaggcaata agacagtcaa cagccaagaa atcatctctg atacaaaatt
    88321 gtcattagga gaaaatgttt ggggacagta ttttcatcga tctacttata tcgatcgcct
    88381 taaaaaagca cagccaagaa tcgaaacagc aaatatacat ttcaaaggga tgaatgaatt
    88441 cgatttggat gtaacagaat ataaagagat tgctttgatc gcaaaaaaca atcaatacta
    88501 cccagtgatt gaaaatggaa cagtattaga tgaaaaagta gcaaatccta caaaaaatct
    88561 ccctatcttg gaagagttca aagactctgc aaaaatcaaa gaattaacaa agcaatacaa
    88621 tcagttatca tcagaattac aaaaagcaat ttctgaaatc aagtacacgc caagagcaag
    88681 caataaaaat ctgatccaat tgaatatgaa tgacggcaat caagttattg taaatatcaa
    88741 taatcttgcg aaccaaatga aatattattc gcaagtagcc aaagatatgg atgaaaaagg
    88801 tgtgatcgac atggaagttg gtatcttctc gcatccttac ggtagcactc aagaaacgga
    88861 agaatcgggc caatcggaag aaacaactga aagtgaagaa aatgcggatc aaacaaacga
    88921 atcaagtgaa gaggcagaaa cgcaaaaagc acaaacacct ggatcgtcta gcacaaacgg
    88981 atcatctgat tcaaatgatt caagtgaaac aagtcaaata gaagataatc caagtacaac
    89041 tccgtcaagt ttaaatgaaa attgataaaa caactttaaa aagtaagaat gtactttcta
    89101 aaaaccagta atttatggta gaattggaag cagtgaatat atctattaat aagtatattt
    89161 taaaacggac gctagttgtt cgcttgtata tggaatagta agtaggaggg aatcccattc
    89221 atggcaaaaa caggaatgta tgtaggcctt gatattggaa cgacatctgt taaagttgtc
    89281 gtggctgaat atatcgacag ccaaatgaat attattggag taggaaacgc aaaatcagaa
    89341 gggattaacc gaggcattat cgttgatatc gataaaacgg tccaagcgat acaacgagca
    89401 gtacgacaag cagaagaaaa agcaggtatc cagatcaaag gcgtaagtgt cggtttgcca
    89461 gcaaacatgc ttgaagtaga aaattgccag ggaatgattg cagttaacgg agattcaaag
    89521 gaaatcaccg atgaagatgt cagaaatgtg gcttcagctg cccttgttcg ttcaatacct
    89581 cctgaacgtc aaatcgtgtc aatattgcca caagatttta ctgtcgatgg gttcgaggga
    89641 atcaaagatc ctcgtggaat gatcggcgta agattagaaa tgtacggtct gttgtttaca
    89701 ggacctaaaa cgatcatcca taacatacgt aaatgcgtgg aaaatgccgg attaatcgtc
    89761 aacgaaatgg tgattactcc tttagcgctg actgaatcaa ttctttctga cggagaaaaa
    89821 gattttggta cgatcgtcat tgatatgggt ggcggccaaa caacaacggc tgttatgcat
    89881 gataagcaat tgaagtttac caatcttgac caagaaggtg gcgaatttgt cactaaagat
    89941 atctctattg tattaaatac gtcatttaac aatgcagaag cgttgaaaat aaactacggg
    90001 gatgcatacc cagaacggac atcaccagat gaagaatttc cagtagatgt gattggtcag
    90061 tcagaaccag tgaaagtcga tgaacgctac ttgtcagaaa ttatttcagc tcgaatggaa
    90121 cagattttca acaaagcaaa agatgcattg gaccaaatcg aagcattaga gttgccaggt
    90181 ggcgtcgtat tgacaggcgg agctgctagt cttcctggtg ttgtggactt ggcacaagaa
    90241 atcttcggcg taaatgtaaa attgtatgtt ccaaatcaca tggggctgag aaacccggta
    90301 tttacaaatg taataagtat cgtggattat tcagcaaact tgagtgaagt gtatcaattg
    90361 gcaaaaattg ctgttacggg tgaaacctca gcagctcgtc aaatggtagt tgaacaagaa
    90421 acaacaaaca catatgagag ctatgaagca cctgaagaaa caatctacga tgagccggaa
    90481 ccaaaagagt ccggagaaaa tgtaaaaaat aaaatcaagg gtttctttac aaatattttt
    90541 gattaattca tcctgggagg aataagatat ggaattctca attgataata atatcaatga
    90601 cggcgcagta atcaaagtca tcggagtcgg tggtggcggt ggaaatgctg taaaccgaat
    90661 gattgaagaa aacgttaaag gtgttgaatt cattacagcc aacacagacg tgcaagcatt
    90721 aaaaaattca aaagctgaaa cagttatcca attaggcccc aaatatactc gtggtttagg
    90781 tgctggttct cagccagaag ttggacaaaa agcggcagaa gaaagtgaac aatccttacg
    90841 cgaagcttta gacggtgcag atatgatttt catcactgca ggaatgggtg gaggaaccgg
    90901 tacaggtgct gcaccaatcg ttgcaggtat cgcgaaagaa ttgggcgcat tgactgttgg
    90961 tgtcgtgaca cgtccattca cttttgaagg accgaaacgt ggccgttttg ctgcagaagg
    91021 tatcgctcgt ttgaaagaaa atgtggatac attattaatt atctcaaata accgtttatt
    91081 agaagttgta gataaaaaga caccgatgct tgaagctttc cgtgaagcag ataatgtttt
    91141 acgacaaggt gtacaaggta tctctgattt gatcacagcg ccaggttacg ttaaccttga
    91201 ctttgctgac gtgaaaacag tgatggaaaa ccaaggtaca gcattgatgg ggatcggtgt
    91261 ggctagtgga gaagatcgcg tagtagaagc aacgaaaaaa gcaatctcct ctccattgct
    91321 tgaaacatct attgatggag ctgaacaagt acttcttaac atcacaggcg gtttggatat
    91381 gacattgttc gaagcacaag atgcttcaga tatcgtagca aatgcagcaa ctggcgatgt
    91441 aaatatcatt ctaggtacat ctatcaatga agaaatgggc gatgaaatcc gtgtgacggt
    91501 catcgcaaca ggtatcgatg aatcaaaaaa ggaacgcaag tctagccgac cagctagaca
    91561 agcacaaatg caatcacctg ctcaaaagac tgtattagat atggatcaag ctaaaccaac
    91621 ttcttcagaa gaagaaaaca gttttggcga ttgggatatc agacgtgaac aaaatgttcg
    91681 tcctagagtt gatgattcca attttgacaa tatcgaaaaa aaagaatttg atacattcaa
    91741 ccgcgaagaa acaaaatctc atggcgatga tgaattaaat acgccaccgt ttttccgtcg
    91801 taaaaaataa aggagaggaa gagcacatga ttgctgacaa cttgcagaaa atcaagcaag
    91861 aaattcagga ttcgtgtgct cttgttcatc gtgatccgaa agaagttaca ttagtagctg
    91921 taacaaaatc tgttgaatcc gctcaagctg aagaattggt tagagctggc gttatgaatt
    91981 gtgcagaaaa ccgagtagat aaattattag aaaagaaaca agatttattt gattttcctg
    92041 aaataaagtg gcatttaatt ggtaatttac agcgcagaaa ggtaaaattg attgtaaatg
    92101 aaattgatta ctttcatgcg ttggacagtt taagattagc agaagaaatc cagaagcggt
    92161 tagagaaaga attatcctgt tttattgaag taaatgtttc tggtgaggaa tcaaaacatg
    92221 gaatttcacc agatgagctt ttaccgtttg tggaatcttt agctgcctgc acaaaaatca
    92281 aggttgttgg gttgatgact atggcgccta aagaagcttc tgatgagcaa atccataaag
    92341 tgtttggtac gctgaaagaa ttgcgtgatc ttgttcaagc aaaaggttat gcccatgcac
    92401 catgtagtga attaagtatg gggatgagtc aagactttca aattgcgatt gaagaaggtg
    92461 caacatttat ccgtgtagga acagcgctat tcaaagacga ataaaggagg aacaaaaatg
    92521 ccgcttttaa acagaggagc aattgctaat tttttcggat tagctgatga ggaagagtac
    92581 gaataccaag attatcctga aacaacaaaa aaggctgtac agccaactaa aaaaaataca
    92641 acacagaatc aatcaactca ttctgtatct gctgcgaata ctcatgaaaa acaaaaaacg
    92701 gcgacttcac gtcctgtaac gcatgaaagt attcagccgg ctgaaaagaa aaaagtaatt
    92761 gaactacatc aatcacctac aaaaactgta gcgaaagaaa gaacagcttc aagaacctct
    92821 acacaaccta aagcaaatct tgctggaaaa atcactgtaa tggaaccaag agcttattct
    92881 gaagctatgg cgattgctaa acgaatcatt gctggagaag caaccttagt gaattttcat
    92941 ttagtagaag aaaaacaagc gagaagaatc gttgattttc taacaggaac agtttatgca
    93001 ctagatggag atatcaaacg tgttggagat gagatttttc tttgtacacc gactgggatg
    93061 gagattgaca gttcaaccgc tcaatctttt gcagatgcga atttgtttga tctgtaagag
    93121 gaggaactac gatttatact attgcactat tacttcattt acttcaacgg gcagcctatt
    93181 tctatagcat tatcttgatg gtatatgcat tgctctcttg gtttccaggc gggtatcaat
    93241 cagggctagg acgattatta gctaagatct gcgaaccgta tatcagtttg ttcgatcgtc
    93301 tgcctttgag gattggaccc ttggatttca caattttagg ggcaatcctg atcttgaact
    93361 tagccgtaca aggtttagca cagatcgtta tatggataac tgtccattta gtttattgat
    93421 tgaagcaaca aaatctttat tctcagaata gataggaggg gaaaattttg gatgcaaatg
    93481 tataccagca ctttcgggca gatgaacgta catttatcga ttcggtaggc gattggattg
    93541 aacaggttca aggacaatat gcaccctatc tgaccgaatt tcttgaccca agacaatcct
    93601 atattttaga aacactgatt cgacaaagca gtgatttgcg tttcatgttt tatggcggat
    93661 atgaacaggc agaaagaaaa agatgtttga ttttccccga ttattacgaa cctgtagagg
    93721 aagattttga aacagcattg tatgaaatcc attatcctag caaatttgcc agcctaagtc
    93781 atgggaaaat ccttggaact ttagtgggaa cggggatcaa acgctcttac tttggcgata
    93841 tcatctcgga cggtgatcgc tggcaagtat ttattgcaaa tgaggtggat cattttgttg
    93901 ctgcacaagt aacaaagatt ggaaaggttg ctgtacgctt ggaaccaatc aaatacacag
    93961 aaatgattat gccgaaagat agctggacac aagaaagagg aacagttagt tccatgcgac
    94021 tagacagtgt gatctctgag atttataata tttccagaca gcggtccaag cagctgatcg
    94081 aagctggtaa agtaaaagtg aactggacag aaaatacaag acctgatttt gtacttgaac
    94141 tattagatat tatttcagtc agaggttatg gcagagtcca gatccaagaa cttgagggga
    94201 agacgaaaaa agaaaagtac agggtactgt tcggtgtatt acgtaaataa aataaaaagg
    94261 tggtttactc atggctttaa ctccattaga tatccaaaat aagacattcc cgacaaaaat
    94321 gcggggatat aatcaagacg aagtagatga tttcttagat ttagttgttc gtgactatga
    94381 ggaattgact caaagaaatc gagaattaga aaaagcagtc aaacattctg aagaaaaact
    94441 tgagtacttc aatgaattaa aagatgcttt gaaccaatca atcatcgtag ctcaagatac
    94501 ggctgataaa gtaaaaacaa gtgcaagcaa ggaatccgaa gtgatcgtta cttctgcaca
    94561 aaacaaagca gatgagttag ttgcgaacgc tgaaaaacgt gcacatcaat taacaactga
    94621 tgcagaagaa aaagcaagaa aaatcttgac agatgcaaca gaaaaagcaa gacagcttgc
    94681 gacagaaact gaagatctta agaaaaaaac acgagtattc catcaacgta tcagcttgat
    94741 gcttgagtct caattagaac aagtgaaaag tccggaatgg gatgaaattt tacaaccatt
    94801 ctccagctac gtgactgaca gccatgaagt gatcaaagaa gttttagcaa aacagcttga
    94861 caatgaaaat gatactgatg taaactcaga cacaggagtc atagaagcac ctatgacggc
    94921 atttgataca caggctattg atatctcgat cgtgcctggt gaaaatgaaa acgagtagaa
    94981 cagaaaagca ctttgttatt cttaaagcga gccgatgaga gtggaagttc ggtaagacac
    95041 tgcatggtga aagatcctct acttgagacc tgcttttccg aaattagagt aagaagcagc
    95101 ggctgacctc gttattggtc acagagagtt tgcactagat agatgcaact aaatttgggt
    95161 ggtaccacgg catgttcgtc ccttgcggct gaacatgctt ttttgtgtcc ggaaaacaaa
    95221 taaaggagtg tatggttgaa tgaaaatgaa agaaaccttg caattaggaa aaacaaaatt
    95281 ccctatgcgt ggtaatttgc caaaccgaga aggcgagtgg caaaaagaat gggaagaaaa
    95341 tcgaatctac caaaaaagac aagagttgaa cgaaggaaaa cctagctttg ttcttcatga
    95401 tggacctcca tatgccaatg gtaatatcca tttaggacat tcattgaata aaatcagtaa
    95461 agacattatc attcgttcaa aatcaatgtc tggctatcgt gcaccatatg ttccaggatg
    95521 ggatacacat ggattgccga ttgaacaagt attggctaat aaaggcgtga aacgtaaaga
    95581 aatgtccatg gcagaatact tggaaaaatg tcgtgaatat gcactttccc aagtagataa
    95641 gcaacgagca gatttcaaac gcctaggagt tgcaggagat tgggaacatc cttatgttac
    95701 attagatcct tcttatgaag ctgcacaagt acgcgtattt ggtaaaatgg ctgaaaaagg
    95761 ctatatttac aaaggattga aaccaattta ctggtctccg tcaagtgagt cttctttagc
    95821 ggaagctgaa attgaatata aagatgtaaa atctccatct atttttgtag ctttcaatgt
    95881 caaagacggc aaagatattc ttgatgaaga tacagctttt gtcatttgga cgacaacacc
    95941 atggacattg ccagcaaacc aaggtattgc tgtaaatcca aaatttacgt atgtattggt
    96001 ggaagccgat ggccgtaagt ttgtcgtagc gaaagatttg ctagaaacag tccaatcaga
    96061 aatcggttgg gaaaatgtaa cagttctaaa agaatttgct ggacaagaaa tggaatatat
    96121 gactgcaagt catccattct atgaacgtga atcacttgtg attttaggcg atcatgttac
    96181 tttagacgct ggtactggat tagtccatac tgctccagga catggggaag atgactatat
    96241 cgctggtagc aaatataaac tatctgtagt gtcacctgta gactcaaaag gattgtttac
    96301 agaagaagca cctggatttg aaggtatctt ttatgacaaa gcaaatccta tgatcactga
    96361 tctattaaaa gaaaaaggag ctttgctgaa gctagatttc tttactcata gttatcctca
    96421 cgattggcgg acgaaaaagc ctgtgatcta tcgggcaact ccacaatggt ttgcatctat
    96481 cgataaattc cgtcagaata ttttggatga agtagaaaaa gtagagtggg ttatcccatg
    96541 gggtaaaaca cgtttgtata atatgatccg tgaccgcggc gattgggtaa tttcccgtca
    96601 gcgtgcatgg ggagtaccac ttccgatttt ctatgctgaa aatggagaag caatcatcac
    96661 accagaaaca atcgaacatg tggcaaaatt atttgctgaa catggatcaa ttatctggtt
    96721 tgaaaaagaa gcgaaagaac ttcttcctga aggatttacc cacccaggtt ctcctaatgg
    96781 cgaatttaca aaagaaaaag atatcatgga tgtctggttt gattctggtt cttctcatga
    96841 agctgtttta cgtcagcgac cagaattatc attcccagca gatatgtatt tagaaggatc
    96901 agaccaatat cgtggctggt tcaactcaag tatcacgact agtgtggcta tcaatggtgt
    96961 agcaccatac aaatcagtta tttctcaagg atttgcttta gatggtgaag gccgtaagat
    97021 gagtaaatct ttaggaaaca tcattgcacc agaaaaagtg atcaaacaat ttggtgcaga
    97081 tatccttcgc ttatgggtat ctagtgtaga ttatgaagca gatgttcgtg tttctatgga
    97141 tatcttaagt caagtggcag aagtatatcg taaaatcaga aatacgatgc gtttcttatt
    97201 agcgaataca gaagatttcg atcctaaaaa agatactgtt tcgtacaatg atttacgttc
    97261 tgtagacaaa tatatgaccg ttcgattgaa ccaagtaatc aaagaaatcc gagaagaagg
    97321 gtacgacaaa tataacttca tgcatattta ccgtacagtt atgaacttct tgacggttga
    97381 tctatcgtca ttctacctgg attttgctaa agatgtagtt tatattgaag cagaagatga
    97441 ttatcaacgt cgttgcatgc aaactgtttt ctatcaaaca gctgtagctt tgacaaaatt
    97501 attgactcct atcattccgc atacagcaga agaaatctgg tcattcctga aagaagaaga
    97561 ggagtacgtt cagttaagcg agtttcctgg ttatgaagag tttgcaaacc aagaagaatt
    97621 gatggatacc tggactgctt tcatggactt ccgtgataat gtcttaaaag cattggaaga
    97681 agcaagaaac agcaaattga ttggtaaatc tttggaagca aaactaactg tttatccaaa
    97741 acaacaagtt cgtgaaatgt taaaagcttt ggatgcagat attgcacaat tattgatcgt
    97801 ttcaccagac tactttgaag ttatggcagc agatgaacaa gtgccagata acgctatggt
    97861 atttgatgat gttgctatta cagtggaaaa agcagacggg gaaacttgcg atcgctgtcg
    97921 ccaagttcgt aaagatgtag gagtagatga aaaacttcct catctatgcg gtcgttgtgc
    97981 aaaaatcgtt gaagacttct atcctgaagc tgtagctgaa ggattcgaag aaaagtaaaa
    98041 gggaaaaggg ggtgtgacat gttctttgtc acaccccctt ttgttatcaa tcgtttaatt
    98101 ttccacgttc acgataccag ttatctggtt gccagatcca ttcgaatccg tctttttcaa
    98161 gtagttcgaa agctgcttta ggtcccattg atccagcttt gtagttggga aaatccggtt
    98221 gtgtttggtc ccaagctttt cgaatgacat ccacgatatg ccaagattga gcgacttctt
    98281 cccaatgaga gaagttcgtt ccatcaccat tcaacgcatc taatagtaat ttttcgtaag
    98341 cttctgggct attttcaacc atttctgcac tatttctaaa ttccaatttg attggttccg
    98401 tttcaaatcc ttgccctact tctttcccat tcaaggataa agaaaatcct tcagttggct
    98461 gaatatagat cgttagaaca ttaggtggaa gtgtcgtatc gtcacatggt tcatcgacag
    98521 atgttttgaa tacattaaca ggaacttgtt tgaatacgat gttgatccga gtcccttttt
    98581 cagtcaaacg tttgcctgta cgtacataga aaggaacacc tgaccaacgg aagttatcga
    98641 tagtgaattt gcctgcgacg aatgtttcag tctctgattg agcatccact ttgtcttctt
    98701 cgcgatagcc cttgaattgt acaccatcca attcaccttg agcatattgc ccgcgaacga
    98761 agttttctaa cacttcttct tcgttataaa tgcggatagc tttcaatgct ttgatctttt
    98821 ctgtacggat ttctttttcg gaaaatgcta ctggcggttc cattgcaagt aatgctacaa
    98881 cctgtaaaat atgattttgc accatgtctt taagtgcgcc actatgatcg tagtagcccc
    98941 cgcgatcttc tacaccgagg ttttcgccaa aagtaatttg gatattgtcg atatagcgat
    99001 tgttccattg agcctcaaaa atattattgg caaaacggat agctgagatg ttttggatca
    99061 tttcttttcc taaataatga tcaatcctga aaatatcttg ttctgggaat acttctctga
    99121 tttgctggtt caattcaaaa gcagactggt aatcagaacc gaacggtttt tctatgatca
    99181 aacgattgaa gccatttttt gtcataatat tttgtgattt caaatgagaa acgatcgttc
    99241 caaaaaattg aggtgccata gccaaataat agacacggtt accgtttaat tgatagtgtc
    99301 catctaatcg gtcagacaat tcttttaatg tctgataatg cgttgaatca tttacatcat
    99361 gggattgata gtaaaaatgg cttgcaaagg tagttgcttc ttcatctgaa ggattcaatg
    99421 cacggattgt ttctttgaca acttcacggt agtgctcatc gctccaggga cggcgagcgg
    99481 ttccgattac agcaaaatgc tcgcctaaat ttccttttct gtaaaggcgg aacaaagacg
    99541 ggtacaattt acgctgggct aggtcacctg taccgccgaa tatcgtaaac aatacatttt
    99601 tttcattaga catgtggata cactccttta tattttatcg acaaaaatcg tgctggccgc
    99661 cttaaagctg acggggattt tctttctatc attttggata gtgatcggtc cttcataagc
    99721 tgcgatgtca acgatctcat attcttcgtt gatattcagg tcaatagtga ctaagtaatc
    99781 caacaattct ttttcatcaa gcactcgtgc gatacgcact tttgtaccga caggatagga
    99841 ctccaatgtt tgtcggcgtt cttcatggac aacttcctct tttttaggaa tcgatcctcc
    99901 atgaggacaa tgttcaggat gattcaaata gttttctaaa tgatctgcaa gatgttccga
    99961 agtcacatgt tccaatactt cagcatcatc atggacttca ttccagctat agtctaaatg
   100021 ttcaactaaa aaaacttccc ataagcgatg tttgcgaatc agactgctag ctttttgcag
   100081 tccgcttgca gtcaattgta caccttgata aggggaatgt tctacaagct cttctttcac
   100141 aagtttagaa atcatttcac tgacagatgc agctgagaca tctagactag atacgatttg
   100201 tttattgttg actttcgttg tatctccacc tagttctaaa atgattttca aataatcttc
   100261 tcgatttggt gtcatattcg atcatccttt catggtaaaa aaccatcatt agtaagtgta
   100321 acaaaaaaaa cgttaattga ctatttttct aaataaaaaa caaaggattt ttagacgttt
   100381 ttacaacaaa aaacgaaaga tgatagagaa gactcattct ttcgtttata aaggatatat
   100441 atatacattg ggagggatct atttttcaga taacaactta tatgaagtgt ctgcagattc
   100501 gtcataaaga gtttgttcgg gataagaaaa gataagataa aaaccatact gatcacgtac
   100561 gactaaacga tattcatcta tttctaccac agatgtgcca agagactgac cgttgatcaa
   100621 aaacgtgtaa tcagatgtaa tctgtaattg ttgtttttgc tggctatcac taaagaacca
   100681 tgtaccttga tactttgaga gtccttctga cttttccgct tgttttttct ttttcaagta
   100741 aataaaactt gagagaacgg taatgagtga accaagaaaa tagatcattt tttttatcac
   100801 caaatcactc ctttatgaga aaataatcgc atagacatat tctttcttct ttatttcatt
   100861 gtaccgaata aaagctaaaa tttctctaag actttcgact ggttttttta ctgtttttct
   100921 acaaattatg ataggataca tagtaggaag aaaggtgaca taatttgaag tatcagcaag
   100981 tggaaatagc tttttttatt gaccgcccga atcggtttat agcacattgt atgaaaaaag
   101041 acggagaaat aattattgcg catgtcaaaa atacaggtag aggaaaagag gtcctgatac
   101101 caggcgctga agtggctatt gtatttgctc ctggacctaa acggaaaacc gcctatgatc
   101161 tgattgctgt aaaaaagaag ggtgactggt ttaatatcga tagtcaattg cctaatcggc
   101221 tggcaattga tggtatttta gacggcacga tccttttacc aggattaact ggtccattta
   101281 cggtatacaa gcgtgaagta acttatcttc actccaaatt tgatatttat ttagagacgg
   101341 tggtcggaca aaaagcattt gttgaagtca aaggtatgac acttgaaaat aaagcgattg
   101401 gtgcttttcc tgatgcgccc acactcagag gattgaaaca tataggagaa ttaacgtatg
   101461 ccgcacaaga tggttatgcc gcttatgtct tgtttattgc tcagttcgaa catcttcatc
   101521 tggcaacgat ccatgaagaa atgcaaccag cactagctga tatggttcga catgcgcagc
   101581 aaagcggagt tcaaatcctc gcctataatt gtcaagtatc ggcagatgag gtaactgtca
   101641 agcaggccat tccatttgat gtagatatcc cttttgaaga tcctatacaa tagaaagaag
   101701 gtaaaataat gatcaactta ggtattatcg gaacaaattg gattactcat caatttgtcc
   101761 aagcagcttt aagcacaaaa aaatatgaat tgacagcggt atattctaga catttggaaa
   101821 aagcacagga atttggttct cattatgaag gggatatcga attcgcaaca gatttgaaga
   101881 cttttttcaa tattactcat atggacacca tttatattgc ttctccgaat agtcttcact
   101941 ttagtcaagc taagcaagca attttggcag gaaaaaatgt gatagttgaa aagccagctt
   102001 tttcgacgcc aaaagaaatg gatgaaatca tccatttggc aaatgaacac aatgtgtatt
   102061 tttttgaagc agcaagaaat atccatgaat caggttttaa aaagattgcc gattacttac
   102121 cattaaaaga cgagataatc ggtgcaaatt tcagctatat gaaatattct tcgcgctatg
   102181 accaagtatt agatggagaa gaaccgaata tcttctcgac tcatttctca ggtggtgcat
   102241 tgatggattt aggtgtttac cttgtatatg cagctgttgc ttggtttgga atgccaaaag
   102301 aagtccatta tttcccaaga aagatttcta ctggtgtaga tggtcttgga tgggggatct
   102361 tacgttatga gaattttgat gtatcgatcc agccaggtaa aatcggtgat tcttatttac
   102421 cgtcagagat ttattttgac tcaggaacgc tagtgatgaa tggagtcaat gcaattgaac
   102481 aagcacaatt tcatgatcgc gcgcataaag aaatcattga tttggcggta acagctgcag
   102541 aaaatccaat ggcagatgaa gcagaggatt ttgccaatgt catggctcat ccgaaagatc
   102601 cagcttgggg attgttgtat gaagagtgga tcgagctagc tagaaatgtg aaccaagtga
   102661 tttatgattt aagaaaaaat ggcggcatca aattcgatgc agataatgag gaagattttt
   102721 aatgaacgaa tttgtacaag tatttcccga aaattggcag aaaaaatatg aggataaagg
   102781 gtttatctca ccttctctta ttcaacgagc agtttttcag ccactaagta ataaacaaag
   102841 tatagtagcg gtttctccta caggttcagg taaaacactg gcttatcttt ggcctctact
   102901 actaaacgtg gaaccaggtg aagcaagtgc gttggtcatt tttgcttctt ctcaagaact
   102961 agctattcaa gtagcagatg ttgcacgtga atggggaaaa gacaaagagt tgaaagtcca
   103021 atctttggtg ggcggagcca acgtcaaacg tcagattgag ggtttgaagg aaaaaccaga
   103081 aatcttgatt ggtacaccag gaagaatatt ggaattgatg aaagcaaaaa aaataaaagc
   103141 acatcaagtg aaaacaatgg tatttgatga agcagaccag ctatttgacg ctgggaacag
   103201 ccaaatcatt gatcaaattc ttcatcaagc tccaacagag tatcagctag cttttttttc
   103261 agcgacagca gatcgttccc ttgaaagcat tgaaaaaatc actgggaaaa cgtttgagac
   103321 aattgacgtg acagcagagg acgattctcg aaaaggactg agacattact ttttacgtgt
   103381 accgatacga aagaaagatg actacttgag acgtctcacg catatagaag attttcatgg
   103441 tctgattttc tttaatcaac tgactgaatt aggcgtaatg gaagaaaaat tgctgtatag
   103501 aggtgtaccg gtaggaagtc tggcctctga tcagaacaag ctactccgaa aagcagcgct
   103561 agaacaattc aaaaagaaaa tggtcagagc acttttgacc acagatgtgg cggctagagg
   103621 tttagatatt acaggtcttc cttatgtagt gaatgcagaa gttccgttaa gtgaagaagc
   103681 ttatttgcat cgttcgggaa gaactggtcg aatgggtaat gaaggaatag taattacact
   103741 cgtacaagat aataatctaa gagatttaaa acggctttta cgtccatcaa atataacact
   103801 tgaagagatt tttttatacg gaggtaagct gcaaactgag cagccaacca aagaagatgc
   103861 agctcctgca gtaaataaaa agtatgcgaa aactttttct ggaaaggagc agaacccaga
   103921 atataacaaa gaaaaaataa gaaaaaaagt taaaaataaa tcaaaaaaag agagaaataa
   103981 aggagcaagg cgcaaataag cgccttttta tgctatgatt atgaaaacgg gaacaaaatt
   104041 ggttgccaga ggaggggtta tgtgaaagaa cgtcaagaag agatcttgca gctattgaca
   104101 gaaaaccaac aaggattgac agcaagtgat gtagctgagc gcttaactat cgatcgaagc
   104161 aatgcaagcc gatatttaag tgaactatac aaggcccatc atattgtaaa aaccgctggc
   104221 cgaccagtcg tttactcttt gccaacggaa aagtcaaaat ctgatgaagt gcatgtcgac
   104281 tcttctacgc aagtgacttt tgaaacactc gttggagaaa atgattcttt aaaagtcagc
   104341 atacagcagg caaaagctgc gattctttat ccaccgcggg ggttgcatac gattattttt
   104401 ggagaaactg gtacagggaa gtcgatgttt gctgaatgta tgtatcattt tgcaatcgat
   104461 tcagagatgc tttctgcaga tgcgccattt gtttctttca attgtgcgga ttatgctcaa
   104521 aatccgcaac tgctctttgg acacatcttt gggatcaaaa aaggggctta cacaggagca
   104581 gctcaagata gtcccggtct aatagccaaa gcagacggcg gtatattgtt tttagatgag
   104641 atccaccgtt taccgccgga agggcaggaa atgctgttta ctttcattga taaaggtatt
   104701 taccgtccgc ttggagaaag tgcacaagta catgaagcat ctgttcagat cattggggcg
   104761 acgactgaat cttcagaaag tttcttatct acgttcaatc gacggattcc tatggcaatc
   104821 accttgccga atcttgcttc acgttcactg gatgagcgtt atgaaatcat ctctcttttt
   104881 atcaaacaag aagcgaatcg tttgaatcaa cgtatcgata tcgaaaaaga agcgattctt
   104941 gccttcatgc tttatgatgc ggaaggaaat atcggtcaag tgaaacgaga cttgaaacta
   105001 gtctgtgcaa aatctttttt acattaccgt actcatcatg agaaaaagct aatcattcga
   105061 aaagaagaac tttcccttca agtacaaaaa ggattactga aaatcaagga aataccggaa
   105121 cgtctcgacc gattcatgga cagcaaaagt cagtatttga catttgaacc aggatttgct
   105181 gatgttgtct ggtcacaaga tccagaacga aacatgcagg tctataacga tattgaggaa
   105241 aaagtcactt ctctttccga aaccggtgtg gagaacattg atctagagac cctcatttca
   105301 aaagatgtgg atgcttattt ccagacatat gtcaaagaat tgactaaaag ttcgattccc
   105361 aaagatcttt tgccagatga aatctggcgg ttaaccaatc aattgtatga tgtggctgaa
   105421 aaacggttag accgtattta taatgaaaaa gctcgttttg cttttgcttt gcatctgcaa
   105481 agtacattgg atcgtgtgag agaaggtcat atgatcgtcc acccagattt gaataacgta
   105541 cggaaaaatt ttaagaggga attccaggta gcaatcgatc tttcaacgat cattgaagaa
   105601 gaagaacaag ttgagattcc ttttgatgaa atcggcttta ttagtatgtt tctttctatc
   105661 aatctaggag aaagtgagcc tgttaaagaa aacaaagtag atgttctcat attgatgcac
   105721 ggcagacaga ctgcttcaag catgttggag atggcacaag aactattagg aacaaaagta
   105781 gggaaagctt ttaatatgcc actggttatg gaagttcaaa caatgtatga agaagtgtta
   105841 caatatgtca aaaactatca agagcatttg acgaatggcc tactcttgtt aacagatatg
   105901 ggatcattaa acaccttcag tgatttgatt tatgaagaga ctggcattcg gacgaaagct
   105961 atcacgatga caagtaccat gattgtgata gaagcgatac ggatggcaag tgttggaaga
   106021 agcttagaag atatttatca aaatatccag ctttcctttg aatcgatcgt acgtgatcag
   106081 ttcaaagcgg aaaagtctga aactttacag cgtaaaaaag ccgtaatcgt gacatgcttt
   106141 actggtgaag gagtagctgc caaattatat cagcgtatta tgccagtgat taatcaaaat
   106201 aaggtagaac tgatccagat gcagttttta gaacgtgaat cattcaaaaa acacatcgat
   106261 gccttattgg aagaatatga gatcaaagca attgcgggaa cagttgatgt tgattatcaa
   106321 aatatcccat ttttttcagc ctatgacgtt tttgatgatg aaaaactaaa tattttaaag
   106381 cgtatagctg atgatgagat cccgacagct aaaattgtcg attcacttgc tggatcatta
   106441 agcgatgtct tttctattga tgaactgatc cgttcattgc aaaagacagt tcagcagatc
   106501 cagaccgatt tgtcgattat cgtggaacct ggagtagata ctggaatcgt tatccattta
   106561 gccttcatgt tagacggatt gatcaaacaa gaaagccatc ggcaatttga gaaactgtct
   106621 attttctcaa aaatttatcg tttggaaatg gatatcgttc gggcaaactt gatggcacta
   106681 gaacgaaaat atcaggtaaa aattccagaa gatgatatcg catttcttac acaaatgttt
   106741 cttgaaaata aaataaaaaa atgaacacac taatcatact gtttacacac taaacatctg
   106801 tgtgcgcact ataaaaaaga gctaaaaagc atgaaaacgc tatttcagct ctttttttta
   106861 ttggcatgct tcttgcgtta tataaaagtg taaaaaaata aaaggagtgt taaaaatggc
   106921 aaaaaaaaca atcatgttag tttgttcagc agggatgagt acgagtttat tggtcacaaa
   106981 aatgcaaaaa gcagcagaag aaaaagggat ggaagccgat atttttgcgg tctctgcctc
   107041 agatgcagat aataatttag aatctaagaa tgtcgatgta ttattattag gtcctcaagt
   107101 acgtttcatg aaagctcaat ttgaacaaaa acttgcgcct aaaggtattc cattagatgt
   107161 catcaatatg caagactatg gcatgatgaa cggagaaaaa gttcttgcac aagcagaaaa
   107221 tctaatgaaa taaatcaagt atgcagttgg gggtataaca acgtggaaga tcaacaaaat
   107281 ctagaagcca tcatggggtt aatcatgttt ggcggtaatg caaagagcga tgcaatggaa
   107341 gcgattgcgg ctgctaaaat cggtgatttt gagttagcgg ataggaaaat cgctgatgca
   107401 gaagaatcac ttatacaagc gcatcatgca caaacagaca tgttgacaca agaagcacaa
   107461 ggaaatcata tgcaagtgac attattgact gttcacagtc aagatcactt aatgacatct
   107521 attgcgttta ctgatttagc aaaagaaatc atcgaacttt atcgtcgcgt atacagcgaa
   107581 taaatacaac ttcttttttg aacggaagaa gaaaaatttt tgaacggatt ttttattttg
   107641 ttataaggag aactctcttt atataaaaaa cttataggag gaagtaaaaa atgaacggac
   107701 tatcaagttg gatggagaaa tacatcctgc cggttgccgc aaaaatcgga gcgcaaaaac
   107761 atttagttgc tttgcgtgat gcttttatcg gcacactgcc cgcaacaatg gcgggatcag
   107821 tcgctgtaat gatcaatgca attattcgtg atttaccgca acagtttaac agtacctatg
   107881 ctgacagttt agcagcagac aaaggaatct ttgccgtatt gaatgtcatc atcggaatca
   107941 atggttttgt ctggaacggt acactagcaa tcgccggatt gatctttgcc ttttcttggg
   108001 ggtataatct tgcccgagct tatggagtaa atgatctagc tggggggatc gttggtttag
   108061 ctaccttgat tcaaggtatt tcctttggtc cagcattatc agcagcatta gatgtgaatg
   108121 ttccttcaaa tgtcgcagat gcaatcaata gttctgagat tggtgcaacg attgcagatg
   108181 gcacactgtc agttggatta tggggttggc taaaactaga tcatttgaat ggaaacgctt
   108241 attttacagt tatgatcatg ggagctattt cagtcattat tttttgtaaa ttgatgcttg
   108301 ccaacattac gatcaaaatg cctgattcag taccaccagc agtatctaaa gcatttgcag
   108361 ccattttacc agcgacgatc gctttatacg tcattgctat tatcaattat atcgtaggtc
   108421 gtgtagctga tgggcaactg atcattgatt tggtgcaaaa atatattgca gaaccattcc
   108481 taggattatc tcaagggttg ggagcagtat tgatcgtcac agtatttgtt caagtattct
   108541 ggttctttgg tattcatgga ccaaatgtat tggcaccagt gctagaggga atctggggac
   108601 aagcacaact aatcaatatt gatattttcc aaaaaggtta taacggaaaa actggtacac
   108661 cagccgtgtt agatgcaatc gaagatggaa aagcttatat gtgggtccgc ggatcgttcg
   108721 atgcttttgc atggtttggc ggatcaggcg gaacgatcgt cttactgata gctatcttga
   108781 ttttctctaa acgtgcagat tatctaacag tagctaaatt atccattggg cctggtatct
   108841 tcaatatcaa cgaaccagtg atgtttggtc tgccaatcgt attgaatgca atcatgttta
   108901 tcccattctt gattgctcca gtagtagcaa cagcaattgg atggacagct acttatcttg
   108961 gcctagtcgc tccggtatcg cagcaagtca catgggtagt accgccgttc ttattgtcat
   109021 tcttagcaac aggagcggat tggcgggcac cgatcgtaac attagtatgt atggtcgtga
   109081 ctttcttgat ttgggcacca tttgtcatct ctgcaaacaa aatggaccct gccgcaggcg
   109141 aacaacaata agatgaacgt gaggagcggg acgctttaag tttcgttcct cataatgaat
   109201 tttcgaggag gaactgaaat gattacaatt gccagtgcaa tgcaacaaga tgaaatcaaa
   109261 gaattgttag aaggatacaa tgaagaagga atgactttta catttaaaga aaaacaagga
   109321 atcaagctgt tctttgaaac aagtgcagaa gatccggaag ttgcggctaa aaaagcaaag
   109381 gaactaatca aagcacagcc ttggggaaca gtcctttatt ttcaatcaac agctgtttag
   109441 aaaatggttt aaaaagatag gatggttgag atgataaaaa acggtttatt tttatgtagt
   109501 attggttctt tgatcgtgtt gtatagtttg aatccacatt cgcaaaggta tgatttgtat
   109561 agtatggtca caggtctttt tctaacagga ttaggaatct atttcttcat gaaggggaaa
   109621 aaaagagaag aagcaaagga gaaaaataac taatggaaat cacgaaaaaa ttaaatattc
   109681 cagcatcgtt tttttacgat aaagttatgg actctgtcct tttcgatgta cgtaaagcga
   109741 ccggcaaatc agtaactaga aaacagctga aaaatttgga gtacgtcaaa caattctcgc
   109801 aaaataaccg ggctcgcata aaaatcgaag aagtcattga aaaccagtct tataagtttc
   109861 gtacatcgac aacgaagaat gaattcgtcg ttcattatca aatcaaacca ttggacgatt
   109921 cgacctgtga ggttcattac acagaaaaaa tggagtctta tggcatgctt caaaagttga
   109981 atgatatgct tttaggaaca atcttgatgt attttaaacg gagacgtttc aaaaaaatgc
   110041 tagatatgat cgaagcaacg tattaaatca atgaaacatg gtactattta gttattgaaa
   110101 aatgaagtaa gctagaagga atttttcata ggaagataga tctttcctgc gaatcctcac
   110161 tagctttttt gtaggaaggg gcagcaaaat gacaaacaaa gtaagagagc taggtgtatc
   110221 ttcgattgag gaaatgtttg atttagccac ttatgcattt aatggggcag atacacctga
   110281 aaggcgagaa cgttttcgta cacttgcgga gaattcttgg aattacggat tttttgatga
   110341 ggaggatcgg ctaacaagtc aagtcatggc gacccctttt ccagtgaatt tttacggaca
   110401 agagtaccta atggccggca ttggctatgt tgcttcttat ccagaagcaa ggggacaagg
   110461 cgggatcaat cagatcatgg aaaagatcct tgaagattgc cgtgatcgta aagtaagctt
   110521 atcttatctg gcgccattct cctatccctt ctatcggcgc tatggctatg aacaatcatt
   110581 tgataaaatc agttatcaac taaacagtcg tgatgtgccg tttatcaaaa aaaaatctgg
   110641 aaaaataaag cgtatggatt ttgaagatgc aaaagctgca atcagaagaa tttatgaaca
   110701 gatgccggaa aaccaacgag ctggtctgaa gcgcgaagac tggtggtata cgtataaatt
   110761 caagcaaaaa ggcaattacc agtttgcttt gcattttgat gataatgagg aagcaacggg
   110821 atatatcgtt tatcagcttg caacgtcttc ctttattatt gcggagtggg gatttttgga
   110881 tcatgatgct ttttgcagcc ttgttcgctt cgtaagttct cacaatggtg cttttgaaac
   110941 atttgaatat tcatgcggct acggcggtaa tgatcaaaat tatctattag aaaatccatt
   111001 tgcttctgta atgatcacac cttatatgat ggcgaggatc gtagatattt ctttgttttt
   111061 ggaaaattat ccatttaaaa agagacaagc tgcatttgcc ctacaaatag aagaagatca
   111121 ttatgcgaaa tggaatgaag gaatcgtgga agtaacaatc gaaaatggaa aaaatcatct
   111181 ctcaaaagta gaacaaacaa aactcccaat cgttcgagga agtatccaac atatcacgca
   111241 gctattatta gggtatcgta aagtggaaga gttggtattc caagaaagaa tacaggtgac
   111301 aaaagaatat gaagaaacgt taagctcaat cttgccacag caacagccaa ttcttaatga
   111361 ctatttttga taaaaaacgg gtgatgacaa tatttttgtc accacccttt tgtttttcat
   111421 ttggcatttg aaggaaaatg ttaaagtaaa tcctcttttt tcgtaaattt gaattttttg
   111481 ttttcttttg ttttagggtt ttcttttttg tggtcttgga ttgcttcttt cgtaatcttt
   111541 tcccgatttt ttttagatga atctaagcgt tctgcgtata ctttttcttt tggcttatca
   111601 ttcattggaa aggacctccc tacattattt cattctacct ctagtttatc atctgttaag
   111661 gactagtgga aacgttttgc ctaacttata agctgatttt tttagcagat aagcttgcaa
   111721 gtaaaaaaca aacaagctac aattgattta ttgaaattgt tcctgtagct cagtaggata
   111781 gagcaatcgc ctcctaagcg ataggtcgct ggttcaattc cagtcaggaa cgtacaaata
   111841 acccctgtat cctttgcggt acagggggtt attttgtata agtagacatt gtagtttcta
   111901 aactgctatg tcctaaacgt ttcgatacgc taagtatatt tactccttga taaagtaaaa
   111961 tagatgcatg cgtatgtctt agtccatcaa taaacagtcg aagagaaaat agggaaaaat
   112021 tgattactaa agaaaaactt actaggtcga cggtgtagtt gattttcata cttcaataga
   112081 ttcgaattaa acgatttcga ttggcttata ccagtcctat ccaacgaaag atttatcgtt
   112141 cttaactcta gcggtataac ttgaagcttt acgtaaagcc aatgggaatg aactggagat
   112201 gactttttat tatttttagt aatagctgat gggttaatac ttacaacttt ccttttcaaa
   112261 gtaccatata aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt
   112321 agatccttta ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc
   112381 caatcgccat ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc
   112441 atcttgtcag atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct
   112501 gctaattctt catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct
   112561 agtgctaagt gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat
   112621 tgagaaatag gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca
   112681 tctgtatcaa gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact
   112741 tttccatctt taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc
   112801 tttgtacttg aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc
   112861 gtcggcatat ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa
   112921 agatggtttt taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca
   112981 gaaaattgtt ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc
   113041 catgctaaga cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat
   113101 gcctcaactt gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca
   113161 gccgcagcca cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt
   113221 agtgctttgc cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat
   113281 gcatcgggat cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc
   113341 gcttgatcga ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga
   113401 aattctcttt ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta
   113461 taagcttctt gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca
   113521 actgtgtgtc ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt
   113581 agaagttcat taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa
   113641 ctattagcat gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga
   113701 ggttattttt ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg
   113761 aagaaaatca aaaactaatt ttgcatatca aaaaaacatg ttacaatgaa attaatcaat
   113821 ggtggaagcg ttataaaaaa attaaggaga ttaaaaaata tggcaagaaa aaagacgatt
   113881 actagagaac agattttggc agctgcttat gaagtagctg ccaccgatgg atttacacga
   113941 tttactgcga gaaacatcgc gaacaaaatg aattgttcca cgcaaccgat ttatcttgag
   114001 tttaaaaata tggatgactt gagacaagct ctttttgagc agatctatga tcatttacaa
   114061 tatgaagtgt tcactgttga gcatacaggg aatacgatta tcgatcttgc ttttaattat
   114121 atccattttg caagaagaga gaagaagttg tatcgttcac tgtgcttaga agaatatggt
   114181 ggaggaaaag agatgcagaa tttctcttat acttactttg tgaatacagt gaaacgcgat
   114241 ccagcttatg tgaatctttc agaagagcag atcgaatctt tgtataatgg gacatggatc
   114301 atagctactg ggatcgcagc tttgatgtct tcaagcatca tccatccaac tgatgaacag
   114361 attgaaaaga tgatccaaga cagtatagat gctatcttga aattcaaaga accaatcgaa
   114421 atcgattgaa tatactcaaa agttccacaa atcagtaggt aacaagagat gtgtcaaaat
   114481 aaggaaaagt tcgtttttct tacagcgtat gcatttttaa ctcccgaaga actcttccat
   114541 gaatgcaatg aataaaagag ccatacagat tgaataaatc tggatggctc ttttattgca
   114601 agtatattaa atttttgcga ctagttcttt agcgaaagct tctaaatttt cgatatcttc
   114661 ttcttcagca ttcagatcca ctttaacatt ttcggctcct tttgatgcgc ctgtttttgc
   114721 aaaagcagca tcgaaatcat ccaccgattt gcagaattca tcgtaaaaag tatcaccaga
   114781 tccgcagaca ccgtaaattt ttcccaatag atccaattct tgcaagtctt cataaaagtc
   114841 tacgatctca tcaggcagat cgccatcatc gtatgtataa gtagctacta cgcaaatatc
   114901 tgcctcctcg aattcatctg catctacttg ggtacattcg ttgatttcga cttcgatatc
   114961 cagattttca aatgcttctg caacaatatc ggcgatttct tcagtgtttc ctgtcatgct
   115021 agcatatacg atttttgcta aagccatgat gtgcctcctc gtttataatt atttaacaat
   115081 tatgaattat accaaaacat cattgaaaag aaaataaaac ctaaagacac ctggggaatg
   115141 tcatttggct aaatgtatgc cgctatgtta aaatgagagg gatttgtaaa aaggagacga
   115201 aaatatgatt actttaaaat cagcacgtga aattgaaatg atggcagaat ctggagcttt
   115261 attagcagat gtacataaaa atctgcgtga ttttataaaa ccaggaatca caagttggga
   115321 tattgaggta tttgtcagaa actatatcga gagtcatggc ggaattgctg cgcaaattgg
   115381 ctttgaaggg tatgaatatg cgacttgtat cagtataaat gatgaaattt gtcatggatt
   115441 ccctcgtaaa aagcctttga aaaacggtga tttggtaaaa gtcgatatgt gtatcgattt
   115501 gaaaggcggc atttctgatt cttgttgggc ctatgtagtt ggagaatcta cacctgagat
   115561 cgaccgattg atggaagtaa cgaagaaagc tttgtacatc ggtattgagc aagcacaagt
   115621 agggaatcgg attggtgaca tcggtcatgc tatccaaact tatgtagaag gcgaaaatct
   115681 agcgatcgtt cgtgatttta ttggtcatgg cgttggtcct acgattcatg aagaaccagc
   115741 agttcctcat tacggagaag ctggaaaagg gttgcggttg aaagaaggca tggttatcac
   115801 gatcgaacct atggttaata ccggaacttg gaaaatgaaa atggatccta acggctggac
   115861 tgcgtataca agagacggcg gactttcttg ccaatatgaa catacattgg ctattactaa
   115921 agatggtcca agaattttga catcacaagg agaagaaggc acttattgat ctatccagtc
   115981 aataggtacg ggagaggtac atgaaagttt ggaacaaaat aaaacaaaat gaaaatctga
   116041 tgcgctttat tgagacgact caaaagcgca tcactgattc agaaatgagt acgacctctg
   116101 tcgtcgttgc ttattattta ctcttgtctt tatttccatt gattattgct tttggtaata
   116161 ttttaccgtt tctccacatc gatcaagaga ctgtactgac ctatttacga gaagtgattc
   116221 ctgagaccgt ttaccagttt atcggtcctg caatcaaaga ccttttgaca caaagttcag
   116281 gaggcttgtt atccgtttct gcattagcgg ctttatggtc tgcaagtcaa agtatcaatg
   116341 ctttgcaaat tgcgatgaat aaagcgtatg gcgtagaaaa tagaaaaaat tttttgatcg
   116401 tccgtttctt ctcactaatt gtcattgtct tgttcatgat tgctattagt ggggtaacta
   116461 tcgttcttgg gttgggacag ctgattttgg atgctattca gccaatcgta caaatacccg
   116521 tgaactttat cgatcagttc caaactttga agtggccaat cacattggta gctttatttg
   116581 tcatcatgtt cttgatttat ttaattgtcc ctaatgctca gttgaagttg aagacgatca
   116641 ttccgggcac gatcttcgct actgtcggct ggatgctgtt atcacaagtg tttagtatct
   116701 atgctaggta ttttgcaacg aggatcagcg gttatcagat cataggaagt tttattgtac
   116761 tcatgctttg gcttaatttt gctgctacga tcatcatttt gggcggaatc atcaatgcag
   116821 ttgttcaaga atacgtgacg ggtcatcaaa tcaaaaaacg tcgagatcca agccgaaaat
   116881 ggttcaataa attgaaaact aaattttctc ataaaaaaaa gtagaaacaa gagttcttat
   116941 tgtaaattta tatcagtaat ttataataat tatgactctt aattaaagta ggtgcccata
   117001 tgcttgtttc ttttagtatg gttataagat tttttctgac aattttatta tcactgatat
   117061 taacacctgt attcaaaatc atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa
   117121 gaagaataaa taaagttcca atgccttcag cgggcggact gcctatcttt atttcatttg
   117181 ttatatcgac tttattctta ttccgtaaaa ttatccctca atcgtatatt gtaccgatct
   117241 tgattgcttc tttagtaatc atagtcacag gagtgctcga tgataaatat gagctgacac
   117301 ctaaacaaaa aagtataggg atcttgattg cttctcttat tatttatttt gtagcagata
   117361 tacgtattga ttccttcacg cttcctttta ttggatatat ccaactaggc tggcttagtt
   117421 ttcccgtaac gattttttgg atcttcggta tcacgaatgc ggtcaatctg attgacggtt
   117481 tggatgggct ggcggcaggg atttcattga tcggtttaat tacgatagga atcattggat
   117541 attttttcct ccatgcttct acagtgtata ttccagttgt tattttctgt cttgtagcaa
   117601 gtattatcgg tttttttcct tacaattttt atccggcaaa gatttattta ggcgatacag
   117661 gagctctatt cctagggttt atgatggccg tcctttcact acaagggctg aagaacgtga
   117721 cttttgtttc atcgatttct ttgctgatcg tcatgggagt gccggtaaca gacacgtttt
   117781 ttgctatcat taggagaaaa gcaaaccgtg tttctttttc tacagcagat aaaaaacatc
   117841 ttcatcaccg actaatcagt ttaggcttta ctcataaagg tgctgtcctg acgatttatg
   117901 cgatggcttt gatgttttct ttcacagcta tggtcatgaa ttatactgga agaatcggaa
   117961 cgatcatatt gattattgcc atgttgtttg ctgcaatttt attatttgaa ttgattggtc
   118021 tgatcaatga gaaacatcag tttattttaa aaacattgcg atttttaggg aataaagagt
   118081 accgtacgca agtaaaacag aaatatttga agaaatggaa ccgtcatata gatgaataac
   118141 aaattaaacc agccgatcct tattagtatt gtgacggcaa acagtgataa aatttttcgg
   118201 acactagata attttatttc aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt
   118261 gacaacaatt cacttcccga gtacaaaaaa agactaacag agtataggaa atatccctac
   118321 atcgatatct attttcatga tgaaaattcc ggatttggtt atggacataa tcacaatctg
   118381 atagctagcg attctcgcta tggcattatt tgtaacccag atattttagt ggatgaagag
   118441 acagttacag cattggtctt gttattgaaa gaacatccgg aatgtgcgat gttagcacct
   118501 aagattctta atgaagatgg aacaactcag cacctcatcc gtgagcagct ggctgttttt
   118561 gattacgttt tgcgctttgt gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg
   118621 gctgtgtttg aatgccgagg attgcctact gatgctaata gttatgtacg gatgatctca
   118681 ggtagtttta tggtagctga tattgataaa tttaaagaga taaaggggtt tgatgagcgt
   118741 ttcttcatgt actttgaaga caacgacttg tgtttgagtt ttgaaaaagc aggttacaag
   118801 ttattgtaca caccctttta tgcagttatc catttttacg ggaagggtgc gcatcgaaat
   118861 gcaaaactgt ttcgcgtgtt tttgacttct atgatgaaat ttttcaataa gtgggggtgg
   118921 cgttttttct gatgaaacca gaaatattag tagtcgttgt gctttatcag caactattca
   118981 gtcaatctcc agcttatgga ccattaagca aagcacttag cgaaaagcaa attcagctta
   119041 ttatttacga taatagtcct caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg
   119101 tttatcatca tgatccgcaa aatcctggtt tagcggtggc ttataatttt gcgttgtctc
   119161 aagcagatga aggaacacgt tgtctcgtca ctctggatca ggatacgagg ctagtcgacg
   119221 attattttga actattgcgt aaagtaacct ttacagatga atgtgttgct gcagttccga
   119281 tgattttttc tgggagccga caagtttctc ccctatattc agatcgttat atcaatcgta
   119341 cagctcaagc agtagaagtt gaaggcccaa cagctgaacg aatcatggca atcaattcag
   119401 gtgttgcctg gtctgtcaaa tttctaaaaa agattggcgg gtttaatcct gctttttctt
   119461 tagatttttt ggatcattgg ctgttttgga aagtggatca gttggaaaaa actgtggaga
   119521 tccttcctgt acgaatggaa catgatcttt cggttttaga ctatgaaaaa gtttcaagta
   119581 atcgataccg ttctatttta caagcagaaa atcgatttta tcatgaatat gatcaaaggc
   119641 atttatccag tcaccgacga caattattgt taagaacagc taaacagttc ttgaccgtca
   119701 aaaaccggca tatctggcgg atgactttgc gctcatatgt taagaattgg aaggtgtgaa
   119761 ttcgtgcgtt cggtttgtat agctaccttt aatggaggca gatatttaag agagcagtta
   119821 gatagtattt tatctcagct ttcagcagaa gatgaagtag tcatttctga tgatgggtca
   119881 gtagatgata catgggatat attaactgaa tacgcagaaa aagataagcg aatccagctt
   119941 ttttcggggc ctcgtcaagg attgatcgct aattttggtt atgcgatcca gcagacgaaa
   120001 ggagatctaa tctttttggc agaccaagat gatgtttggt tggagacaaa agtgaaaaag
   120061 atcactgact attttgagca gaatccggat cagctagtag tagtttctga tttaatcatt
   120121 gtggacagcg agctgcaaga gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa
   120181 ggatggctta ataatgtgat aagaagtcac tatattggtg ctggtatggc tttccggtct
   120241 tctttaaaac ccgatatttt gccgatcccg tcagaagtgc cgatgcatga tatgtggatt
   120301 ggtttgctgg gaggaaagaa gactgggttc ctaagagaac cgttaactct gtaccgaaga
   120361 catgatttaa acgcgagtga gattgaaaca cattctaaat tacggcaaaa aatttcttgg
   120421 cgtgttcatc tgattacggc gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg
   120481 tttaaatcct taatatttcc atgacaaata taacaattga tgagataata acaaagtgaa
   120541 atttgaaatc aaaaaggaga gcagatatcc atgaagggaa tcattcttgc tgggggcagc
   120601 ggaacacgtc tgtacccttt aacaaaggca acatcgaaac aattgatgcc gatttatgac
   120661 aaaccaatga tttattatcc aatgtctact ctgatgttgg cgggaatcaa agaaattctg
   120721 attatttcaa cgccacaaga tacaccacgt ttcaaggaat tatttggaaa cggaaatgat
   120781 ttaggactgc agatcgaata tgcagtacaa gaaagtccag atggattggc acaagcgttt
   120841 atcattggag aagaatttat tggagatgat agtgtctgct tggtattagg ggacaatatt
   120901 tactatggtg gcggtctgtc taaaatgttg caacgagcag cttcaaaaga tacaggcgcg
   120961 acagtatttg gctatcatgt caatgatcca gaacgctttg gtgtagtaga atttgatgag
   121021 aatatgcaag ctttgtcgat tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta
   121081 actggccttt atttttatga caatgaggta atttctatcg ctaaaggtat caaaccttca
   121141 gaacgtggag aactagagat tacggatgtc aataaagcat atttggataa aggaaaacta
   121201 tccgtagaac ttatgggacg cggatttgct tggcttgata caggaacaca tgaatcattg
   121261 cttgaagcat ctacctttat cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt
   121321 ttggaagaaa ttgcttaccg tatgggctac atcgataagg aacaattgct ggcccttgca
   121381 cagccattga aaaaaaatca atacggtcaa tatttattga atttggccgc tgaataagga
   121441 gtccttacag atgaaagtaa cagatacaaa attaacagat gtcaaaatcg tagaaatgga
   121501 cgtctttggc gatcatcgcg gtttcttcac agaaagttat tcaaaagcaa aatttgcaga
   121561 acatggattg aaatatgact ttgttcaaga caaccattca ctatcagctg aagctggtgt
   121621 ccttcggggg cttcatttcc aaaaaggaga agctgcacag acaaaattga ttcgggtagt
   121681 cactggagca gttttagatg tagtagtgga tatccgcaaa ggtagcccga cttatggtca
   121741 atgggacgga tttatcttat ctgaacataa tcatcgacag ttgcttgtac ctaaaggata
   121801 tgcccatgga ttcgttactt tgacaccaaa tgtgaacttc ctttataagt gtgataatta
   121861 ctataatgca gaagcagatg ggggcatcgc atttgatgat ccagatttag cgattgattg
   121921 gccaattgat ccatcaagag cgattatgtc ggaaaaagat caccgtcatc cgacattaaa
   121981 agaatttgaa gcagaaaatc catttgttta cggagaaatc taagaattaa ggagaaattt
   122041 tcttatgaaa aacatcattg ttaccggagg tgccggattc atcggctcta atttcgttca
   122101 ttacgtggta aacaatcatc cagaagtgca tgtcactgtt ttggataaac tgacttatgc
   122161 aggaaacaaa gagaacttgg ccggacttcc tagcgatcgc gtagaattgg tcgtaggaga
   122221 tgttgcagat gcagaattag tggaccgtct agtaaaagaa gcggacgcag ttgttcatta
   122281 cgcggcagaa tcccataacg ataattcatt aaaagatcct ttcccatttg tacaaacgaa
   122341 tatcattggt acgtatacac ttttagaagc ttgccgcaag tatgatgtac gctatcatca
   122401 cgtatctact gacgaggttt acggcgatct tccattacgt gaagacctgc caggacatgg
   122461 ggaaggcgaa ggagaaaaat tcactgcgga aacaccatat aatccttcaa gtccatattc
   122521 ttctacaaaa gctggttcag atctgttagt aaaagcatgg gtgcgttctt ttggcttacg
   122581 tgcaaccatt tcaaactgtt caaacaacta tggtccttat cagcatattg aaaaatttat
   122641 tcctcgccaa gtaacgagta tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa
   122701 gaatgttcgt gactggatcc atacgaatga tcattcttca gcagtgtggc tgattttgac
   122761 gaaaggcaga attggcgaaa cctatctgat cggtgcagac ggtgaagaag ataataagac
   122821 cgtgatggaa ttgatcttag aaatgatggg ccaaccaaaa gacgcatacg accatgtaaa
   122881 cgaccgcgca gggcacgatt tgcgttatgc aatcgattcg acaaaattgc gagaagagct
   122941 aggttggcag cctgagttta cgaacttccg tgatgggttg gctgacacga tcaaatggta
   123001 cgaagaacat gaagactggt ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa
   123061 aaatggacaa taatacagag gaaatttcac tgtattaatg gaaaaacaca tgtgaggcag
   123121 gcgctcgcct gtctcttttt taaaataaaa atcaaggagc ggataaccat atgtttttat
   123181 taactggagg aaatggacaa ttaggtacag aattgcgcca tttattagat gaaaaaaatc
   123241 tgccatatgt ttcaacagat gcaaaagact tggatatcac agatgctgag aaaacaatgg
   123301 cttatattac agaattaaaa cccgatgtta ttttccattg cgcagcttac acagcagtag
   123361 ataaagcaga agaagaagca aaagaattag atgagaaaat caatgtcgat ggcacaagaa
   123421 atgtagcact tgcagcaaaa gctgtgaatg cacaattcgt ctatatcagt acagactatg
   123481 ttttcgatgg aaaaaacaaa aatgtagaat acgaagtaga cgaccaaacg aatccactaa
   123541 atgaatatgg tcgtacgaaa ttattaggcg aaaaagcagt agaagagatt ttagaagact
   123601 attatatcat acgaacttct tgggttttcg ggatctatgg acacaatttc gtcttcacga
   123661 tgcaacgcct agcggaaacc cgcgacaaac tgactgtagt aaatgaccag tttgggcgac
   123721 caacttggac acgtactttg gcagaattta tggtatatgt catcgaagaa aaagcaccat
   123781 ttggtattta tcatttgtcg aacgaaaata gctgttcttg gtatgagttc gcaaaagaaa
   123841 tattgaaaga tacagatgta gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag
   123901 caacacgtcc gcagtattca gtcatgagcc tgaaaaagac agaagcgtta ggttttgtca
   123961 taccaacttg gcaagaagca cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt
   124021 aatttccttt ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca
   124081 cgacgaaaag aaaagccaaa agcgaatcag gcagattcga atttggtgct tgacctgttt
   124141 ctaaaactcc gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta
   124201 aacggaagtt cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa
   124261 actgatgtga tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca
   124321 cctttttacc tctcacaagc tgaagaggac ttttcgttgg tgaacgagga ttattcatta
   124381 aacggacaaa acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga
   124441 aaaccattca actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg
   124501 gcgatgaaat tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag
   124561 aggaaaaaag ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag
   124621 tggtggttca tgcaacatgt gggcgatttg attttcttta ctttgggact gatgattgct
   124681 ttttatcatt atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg
   124741 gtggttattt tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg
   124801 gcgtacttgc tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca
   124861 tgggatctgt tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg
   124921 gttcatttct ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca
   124981 ggaaaacggg taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca
   125041 aattggaaaa ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc
   125101 tatcactttt ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat
   125161 agcgaacaaa agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg
   125221 attaccgttg ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca
   125281 ttaagagcca tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct
   125341 tatatttgga gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat
   125401 gcatggtctt atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt
   125461 gaaaccatcg cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg
   125521 agacgcttgt tttacctcgc ttttgtcgcc ttagttatcg tatctggctt tttcgtcaat
   125581 ccagtcgtag aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa
   125641 atcgatcaac aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc
   125701 ccaacattgg gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg
   125761 aagaaaatcg atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga
   125821 gcttttatcg ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc
   125881 acgttgaatt ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca
   125941 ttaactgatt acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa
   126001 gacggttata tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa
   126061 actcaggaaa atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatatt
   126121 agtagaaagg agagtgaaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag
   126181 tgtcggtttc ttgcttttgt tgctgacgct agtctttttt gtttggacag caggggtagg
   126241 ttttccagca actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc
   126301 tattttgctc attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt
   126361 tgcgcttgcc cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt
   126421 tggagactta ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg
   126481 gacaaaatgg gatcgcggta tttatctgat tgtctttacg gtcttcctcg gaatcacgtt
   126541 atatcgtgta tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa
   126601 ttcgctttct gatatttgga tcaatacgaa tacaatcgga agttctttga tgactcttgc
   126661 tttattgatc agtggatttg ccagttcttt tgaacgatgg tatgttcgaa tcatcggtgg
   126721 gatagcagtc gttgcagcct ttttaggtat ttgggtctgc caatctagag gtgcattagt
   126781 cgcgttgatt gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg
   126841 tgcgccattt attgcctaca cggttacgat tcttttagct ttgccgattt cttatctggc
   126901 ggctgtctct gaaaaagtca atttgtttac cgatcgagaa gatatttggc ataaattcta
   126961 tcagacgcta ggagaaaaat ctgaacaagt tttactagga atgaaaacat ttatcttcca
   127021 gcgaggaaac caatttttag gaaaccacaa tagttataat tctattttga atatctatgg
   127081 attgatagga tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt
   127141 gaaagcagat ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat
   127201 gcagtcattt atggaggata cattgacttc atacccatgg gttccgatta tttatatcct
   127261 gttggctatg gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat
   127321 ggaagaggca tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag
   127381 cgctcattct tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt
   127441 gacaaccgtc gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga
   127501 aacgttagag aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt
   127561 accaaaaata tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca
   127621 caaatgttta aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg
   127681 ctacagtttt cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta
   127741 tgggcgttta tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga
   127801 ctgaaagcac aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg
   127861 attccctggt tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat
   127921 acttatttag taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta
   127981 tccaatctgt acacccattt attcttcatt ctgattggca tagctgtcgc gcttataaat
   128041 ggtatatatc cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct
   128101 ttcttgacag gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg
   128161 caattaatta cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct
   128221 aatcagtttg attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt
   128281 caaggttatc gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact
   128341 ttgtatttct gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga
   128401 ttgaaaccac atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta
   128461 gactaaaaga tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg
   128521 aagccctcaa tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga
   128581 atgtagaaat aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat
   128641 ctacaatgtt gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg
   128701 aaggaaaaat ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct
   128761 atgaaaatat ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa
   128821 tggtcgatga tatcattgaa tttgctgaca tcggagacca tatttatcaa cctgtaaaaa
   128881 cttattcaag cgggatgttc gttcgtctag cttttgctgt agcaatcaat gtgaatcccg
   128941 atattttgat tgtggatgaa gcattggcag taggcgatct tgaatttcaa ctaaaatgta
   129001 tggaaaaatt tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata
   129061 tcagttcgat tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg
   129121 aatatggcga tacgatggaa gtaacgacga attatgaaaa ctttttaaag aaaaaatcag
   129181 ttaaaacagt ggatcggaat aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg
   129241 acgtgacgaa agcggaattg ttggataaag acaaaaatcc ggtggatatg ctggaacaag
   129301 gagaaaaact atacgttaaa gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg
   129361 ttttaggaat tgcgctacga acagtcgaca atttttatgt atgcggactt aatactctac
   129421 tagatggtat caagattcct tggaaaaaag gagagaatat cttttatctg gagtatgaaa
   129481 agttaggttt gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa
   129541 cggttccttt ggtatataag acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg
   129601 gagaaggcat cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat
   129661 gattttgaaa tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa
   129721 ccaggaagta aagttttaga gtttggtccc ggaaacggcc gattaacgaa acatttaatc
   129781 ggagcaaaac aatgtgaagt gagcattgtt gagttagata aggaattgtt cgattttgtc
   129841 agcgaatttg ctcaagatgg attttatggt gatatcgaaa gttttgagtg ggcaaactat
   129901 tatgctggac agacatttga ttacgtcttg tttgcggatg ttttagaaca tttggtagat
   129961 cctggaaaaa cattgaagaa agtcagagaa tttttgaatg aagaaggaga gatcctgatt
   130021 acattcccta acttggcgca taattctgtc atgatcgatt tgttcaataa ccaattgccg
   130081 tgggcttctt atggtctcct agatgaaacc cataattctt tttatactca tgatggcttc
   130141 caaaaagttt ttgaaaaagc gggattattc attaatattg aagattattt gtacttagct
   130201 gtaggtgata cagaattaaa atcaacttat gaagaattgc cggaagctgt acgctatgat
   130261 ttcaaaatgc gaccatttgg tgaagtctac caatactttt tctcattgat gaaacatcca
   130321 gtagcacaaa gttccattgc agagccacaa aattctaatt atgtaaaagt gttagaagta
   130381 acccagcaaa cgaaacaaga tgaaacaatc caacaaatac cattcaataa tttcacaggg
   130441 gaaaatgaga cactaagttt ccccgtatca gaaacaacag aaagaatggt gtttcgcttc
   130501 gcgcaacaac ctagctttat cgaattcagc gcagaagctg ccggagaaaa actaacattt
   130561 atcgattcga atgctgtagt caaaacagta aatgattgtt atttgtttga tgggaaagaa
   130621 ttaccagagt tcgttctaac ggatatttct gggaaagaag tgactatcca ttgtcactat
   130681 cgctttatcg gggaattgac accaacaatg aaagaattgt tggaaacgat tcgtcccatg
   130741 gctgaagtca caaagcagct ttctgagaaa aatgacgaat tagaaagaga aaatcatcat
   130801 cttcttgagg aaaatacaag actagaccat acgttaaaaa caaccacaaa tcgctattgc
   130861 acattgttgg aaagtgatga atggacaata aaacatagac taggacgacg taagaaagaa
   130921 acttcgaaaa aaatccaaga aaaagaactt tctatatgta ttgacgagaa aatctgggat
   130981 gcagaaacaa aaatattaaa aatcatcgga tggggaatcg ctaattcgga tcgccagccc
   131041 ttgtcctata agttatcagc tgaccaatcg ccatttttcg aagcaattcc tgtttcacga
   131101 gaagaagtca atcaagcaga acagctcgca aaaggaacag aagcgggatt tgagttacga
   131161 atcctttgcg agcaagaacg ttcattctta gtggaagcag tagctcaaaa tggccaatca
   131221 tggatcattg aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc
   131281 aaaaaaaaga aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt
   131341 tgcttattgt gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag
   131401 aaaaagaaaa tgtgattgct gaatcaacac atgtcgtaag attggatatc aatcatttat
   131461 ataatcttga tgttaagacc caaagcggtt tcaaggtacg tttatcaggg aaaatgagag
   131521 gaaaagccat cttagacttc caaacagcga gacatcaaaa tggcatagct gtgaaattaa
   131581 acggtaaata cccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac
   131641 tgaaaaaagg gataaattat gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca
   131701 aattggaatt gaagccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac
   131761 cagatttgaa agagcaaagg aaagaagcac aaggatttgc gtaccgtcca ttgatttcca
   131821 tacttatgcc tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac
   131881 tggatcaaac gtatgatcat tgggaactat gtatcagtga tgatgcttct actgatccag
   131941 ctatccgaaa gtgtctggaa tcctatcaag caaaagatga tagaatcaaa gtcgttttcc
   132001 gaaaagaaaa tggacacatc agtctggcaa ctaattctgc attggaaata gcagaaggag
   132061 aatttatagc actcttagat aatgatgatg aattaccgcc atttgcatta tatgaagtgg
   132121 caaaagtgct aaacgtacat ccagaactgg atgtgatcta cagtgacgaa gataaaatcg
   132181 atgcagatgg caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct gatacattga
   132241 tgggaaataa ttatatctct cacttaggcg tatatcgaac aagcattgtt aaagaacttg
   132301 gcggtttccg taaaggctat gaaggttctc aagattatga tttggtcctt cgtgtgacag
   132361 aacaaatccc tgcagatcat atttaccaca ttgaccgagt cttgtatcat tggcggacga
   132421 tcccaggatc aactgcaagt aatggcgaag ccaaaagtta tatctatgat tccggagtga
   132481 aagcattgac agatgccctg tctagaagaa acatcaaagg aagcgtacgt cctggcagaa
   132541 tttctggatt ttatgagatt gcttatgacg tcttgcaaga ggatttggtc agtgtgatca
   132601 ttccaacgaa gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa
   132661 cgtcttattc aaattatgaa atcattattg cggataacgg aagtacagat ccaaaaatgc
   132721 aagaattatt tgcagaatac aagcatcagc tgaaagaccg ctttatcgta gaattgattg
   132781 acattccatt caattattct cgaattaaca atttagcagc agaaaaagca aacggcaaat
   132841 actttttatt cttgaataac gatacagaag tgattgagcc agattggatg acggtgatgg
   132901 tttcttatgc acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg
   132961 atacaacgca gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga
   133021 ataactatga tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact
   133081 tagcagtcac tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt
   133141 ttgatgaaac attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac
   133201 tgggtcgtta caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa
   133261 gaggttatga agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc
   133321 aagataaatg gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag
   133381 acggaatcgg cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg
   133441 tgattcgctc gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc
   133501 gactcaaaaa aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa
   133561 attcacgaaa attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct
   133621 tctgctccca ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc
   133681 ccacgctctt attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg
   133741 aaccctagct gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg
   133801 tttaaggtat tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt
   133861 gatggagata accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat
   133921 tgctgaatat atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt
   133981 ttgtaagtct tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg
   134041 tacgatttgt tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag
   134101 atcacttgcc gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa
   134161 ccagacattg gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc
   134221 agcataataa ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa
   134281 atcaacagtt gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac
   134341 agtttgatag ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt
   134401 ttttgtccaa tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg
   134461 tgctttcatt gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt
   134521 cactttatac tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg
   134581 ctctaaagca atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca
   134641 agtatcgtct tttttataac cattccattc atctgtataa acttctaccc catccttata
   134701 tgcgacaatt tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt
   134761 attcataaga tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca
   134821 attttttcga ttcttcacgt aatacagtta ttcttatgga ctagacagcg ctttgggaag
   134881 atggattctc tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata
   134941 cttcgacttc tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt
   135001 tagagtcctt gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca
   135061 ttacaatttg cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt
   135121 attttgtgat taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga
   135181 tattcattat attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac
   135241 tgaagtttcg tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta
   135301 ttatttcagt gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc
   135361 gaatgatcag tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga
   135421 tcatggtagt gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt
   135481 catttgttct cggtacgctt ttcttaagta tctatcgtat tctcgtatat aaactttatt
   135541 tacgcgtaag tcgagataaa aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag
   135601 caatccaaaa ttttggttca aagaaaaaca acaaacataa agtcgaagca gtcattcttg
   135661 ataactatta tgcaaacatc aaaaatatga ttaaagacat cgatattgtc tatcttgctt
   135721 ctcatatcga agaaactgaa aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa
   135781 aattgttttt gaatacaaca tttgaaaact tgacattgat caatccaaat attatgaatt
   135841 tcgaagatga aagtattatt gaagcatctg gatttagaat ccaacctgaa tatgaactat
   135901 tcaaacgttt gtttgatatc atcgtttcac ttgttttatt agttattgct tcaccattta
   135961 tgttattcac agcaatttta gtcaaagtga catcaccggg accaatcatc tataaacaag
   136021 tgcggatcac aaaaaatcaa aaggaatttt caatctataa attccgctcg atgaccgcca
   136081 cagccgaagc aaaatctgga ccagttttag ctaagagcaa tgatgcacga gtaacgcctg
   136141 taggtaaatt tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga
   136201 aaggcgatat gtcgatcgta ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca
   136261 atgcagaaaa cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg
   136321 cacaagtcta tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt
   136381 atatcaaaaa atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc
   136441 tatttgataa aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag
   136501 aaattgcttt tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc
   136561 cgaaattcag cataatcgta ccagtataca atgcagaagc aactattggt cgaacgattg
   136621 atattctaaa agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa
   136681 cagatcaaac acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga
   136741 ttactacgac caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag
   136801 gagaatatct cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt
   136861 atgatcattt gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc
   136921 gtacaatgga cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact
   136981 atctaactca cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt
   137041 atgttgtatg gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta
   137101 aaacttataa tagttgtgag gatcgaatat ttaacttaca ttattatgaa tactgtcatc
   137161 aagtgatcat gaacccaaaa gtagaatata tttatgaatt tgaaggtggg aaggggatca
   137221 ctaatcaata ccgacctaac aagttcgaaa catttaaaga attctattca ttagcaaata
   137281 ctgtaacaga cgatattaat aaagacggta tggcggcgct tcttttaaaa gggacaactt
   137341 cagttatttt ttctatttat agtacctctg caagaacttc aaaagagaag aaagacgaag
   137401 caaagcagat attgagtgat ccagtgatcc gagaagccaa gcaaattgct aagacggatt
   137461 cttcagcaaa aaaaataaca aagttgcttt acaatatgcc agctccacta tttttatctg
   137521 ctgtaaaagt tggaagcttc gttgaagtga aaatgccagg gttaatggct gctttgaagc
   137581 gagtatatta gttaatggag gaaaatataa tgaaaagaat tttgcatttt cagggacgaa
   137641 tgggtttagg tggagcagag tctttcatga tgaatttata tcgtaaaatt gatcgtacaa
   137701 actatcaatt tgattttttg atttatgaag attatgcaga tgttcaagac tatcattcag
   137761 agattgaaag attaggaggc cgtatttttg ttgtacctaa t
//
LOCUS       DACECZ010000002        90249 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.002, whole genome shotgun sequence.
ACCESSION   DACECZ010000002 DACECZ010000000
VERSION     DACECZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 90249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.002"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            11..1009
                     /locus_tag="IWO35_000146"
     CDS             11..1009
                     /locus_tag="IWO35_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315235.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(1249..1332)
                     /locus_tag="IWO35_000147"
     tRNA            complement(1249..1332)
                     /locus_tag="IWO35_000147"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1296..1298),aa:Leu,seq:gag)
     gene            1715..2932
                     /locus_tag="IWO35_000148"
     CDS             1715..2932
                     /locus_tag="IWO35_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0315236.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3116..3952
                     /locus_tag="IWO35_000149"
     CDS             3116..3952
                     /locus_tag="IWO35_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0315237.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            4028..5176
                     /gene="nagA"
                     /locus_tag="IWO35_000150"
     CDS             4028..5176
                     /gene="nagA"
                     /locus_tag="IWO35_000150"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ0315238.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            5396..6454
                     /locus_tag="IWO35_000151"
     CDS             5396..6454
                     /locus_tag="IWO35_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ0315239.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6473..7492
                     /locus_tag="IWO35_000152"
     CDS             6473..7492
                     /locus_tag="IWO35_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ0315240.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7594..8310)
                     /locus_tag="IWO35_000153"
     CDS             complement(7594..8310)
                     /locus_tag="IWO35_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0315241.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(8279..9064)
                     /locus_tag="IWO35_000154"
     CDS             complement(8279..9064)
                     /locus_tag="IWO35_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0315242.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(9055..9804)
                     /locus_tag="IWO35_000155"
     CDS             complement(9055..9804)
                     /locus_tag="IWO35_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ0315243.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            10102..10626
                     /locus_tag="IWO35_000156"
     CDS             10102..10626
                     /locus_tag="IWO35_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315244.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10642..12141
                     /locus_tag="IWO35_000157"
     CDS             10642..12141
                     /locus_tag="IWO35_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ0315245.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(12229..12831)
                     /locus_tag="IWO35_000158"
     CDS             complement(12229..12831)
                     /locus_tag="IWO35_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0315246.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12935..13891
                     /locus_tag="IWO35_000159"
     CDS             12935..13891
                     /locus_tag="IWO35_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ0315247.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(14196..15152)
                     /locus_tag="IWO35_000160"
     CDS             complement(14196..15152)
                     /locus_tag="IWO35_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0315248.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            15326..15886
                     /locus_tag="IWO35_000161"
     CDS             15326..15886
                     /locus_tag="IWO35_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315249.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15879..16703
                     /locus_tag="IWO35_000162"
     CDS             15879..16703
                     /locus_tag="IWO35_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ0315250.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16713..17330
                     /locus_tag="IWO35_000163"
     CDS             16713..17330
                     /locus_tag="IWO35_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ0315251.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            17348..18031
                     /locus_tag="IWO35_000164"
     CDS             17348..18031
                     /locus_tag="IWO35_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0315252.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18714..19949
                     /locus_tag="IWO35_000165"
                     /pseudo
     CDS             18714..19949
                     /locus_tag="IWO35_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            19957..20844
                     /locus_tag="IWO35_000166"
     CDS             19957..20844
                     /locus_tag="IWO35_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0315253.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20861..21697
                     /locus_tag="IWO35_000167"
     CDS             20861..21697
                     /locus_tag="IWO35_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0315254.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21700..22323
                     /locus_tag="IWO35_000168"
     CDS             21700..22323
                     /locus_tag="IWO35_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315255.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            22320..23612
                     /locus_tag="IWO35_000169"
     CDS             22320..23612
                     /locus_tag="IWO35_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0315256.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23971..24068
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            24184..24765
                     /locus_tag="IWO35_000170"
     CDS             24184..24765
                     /locus_tag="IWO35_000170"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ0315257.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24768..26075
                     /locus_tag="IWO35_000171"
     CDS             24768..26075
                     /locus_tag="IWO35_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ0315258.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26368..26856
                     /gene="purE"
                     /locus_tag="IWO35_000172"
     CDS             26368..26856
                     /gene="purE"
                     /locus_tag="IWO35_000172"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ0315259.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26840..27973
                     /gene="purK"
                     /locus_tag="IWO35_000173"
     CDS             26840..27973
                     /gene="purK"
                     /locus_tag="IWO35_000173"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ0315260.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27986..29281
                     /gene="purB"
                     /locus_tag="IWO35_000174"
     CDS             27986..29281
                     /gene="purB"
                     /locus_tag="IWO35_000174"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ0315261.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29461..29838)
                     /locus_tag="IWO35_000175"
     CDS             complement(29461..29838)
                     /locus_tag="IWO35_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315262.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            30094..30822
                     /locus_tag="IWO35_000176"
     CDS             30094..30822
                     /locus_tag="IWO35_000176"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ0315263.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30822..31076
                     /gene="purS"
                     /locus_tag="IWO35_000177"
     CDS             30822..31076
                     /gene="purS"
                     /locus_tag="IWO35_000177"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ0315264.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            31078..31749
                     /gene="purQ"
                     /locus_tag="IWO35_000178"
     CDS             31078..31749
                     /gene="purQ"
                     /locus_tag="IWO35_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ0315265.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31750..33972
                     /gene="purL"
                     /locus_tag="IWO35_000179"
     CDS             31750..33972
                     /gene="purL"
                     /locus_tag="IWO35_000179"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ0315266.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33957..35396
                     /locus_tag="IWO35_000180"
     CDS             33957..35396
                     /locus_tag="IWO35_000180"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ0315267.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35428..36471
                     /locus_tag="IWO35_000181"
     CDS             35428..36471
                     /locus_tag="IWO35_000181"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ0315268.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            36468..37046
                     /gene="purN"
                     /locus_tag="IWO35_000182"
     CDS             36468..37046
                     /gene="purN"
                     /locus_tag="IWO35_000182"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ0315269.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            37043..38578
                     /gene="purH"
                     /locus_tag="IWO35_000183"
     CDS             37043..38578
                     /gene="purH"
                     /locus_tag="IWO35_000183"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ0315270.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38594..39868
                     /gene="purD"
                     /locus_tag="IWO35_000184"
     CDS             38594..39868
                     /gene="purD"
                     /locus_tag="IWO35_000184"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ0315271.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39990..40904
                     /locus_tag="IWO35_000185"
     CDS             39990..40904
                     /locus_tag="IWO35_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ0315272.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(41037..42602)
                     /locus_tag="IWO35_000186"
     CDS             complement(41037..42602)
                     /locus_tag="IWO35_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0315273.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42595..44046)
                     /locus_tag="IWO35_000187"
     CDS             complement(42595..44046)
                     /locus_tag="IWO35_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0315274.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(44060..45076)
                     /locus_tag="IWO35_000188"
     CDS             complement(44060..45076)
                     /locus_tag="IWO35_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0315275.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45296..46384
                     /locus_tag="IWO35_000189"
     CDS             45296..46384
                     /locus_tag="IWO35_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0315276.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46438..47964
                     /locus_tag="IWO35_000190"
     CDS             46438..47964
                     /locus_tag="IWO35_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ0315277.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47966..49171
                     /locus_tag="IWO35_000191"
     CDS             47966..49171
                     /locus_tag="IWO35_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0315278.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49428..50405
                     /gene="guaC"
                     /locus_tag="IWO35_000192"
     CDS             49428..50405
                     /gene="guaC"
                     /locus_tag="IWO35_000192"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ0315279.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50669..52093
                     /locus_tag="IWO35_000193"
     CDS             50669..52093
                     /locus_tag="IWO35_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0315280.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52554..53663
                     /gene="pdhA"
                     /locus_tag="IWO35_000194"
     CDS             52554..53663
                     /gene="pdhA"
                     /locus_tag="IWO35_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ0315281.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53666..54643
                     /locus_tag="IWO35_000195"
     CDS             53666..54643
                     /locus_tag="IWO35_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ0315282.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            54668..55963
                     /locus_tag="IWO35_000196"
     CDS             54668..55963
                     /locus_tag="IWO35_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HAQ0315283.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSASSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            55971..57377
                     /gene="lpdA"
                     /locus_tag="IWO35_000197"
     CDS             55971..57377
                     /gene="lpdA"
                     /locus_tag="IWO35_000197"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ0315284.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            57539..57814
                     /locus_tag="IWO35_000198"
     CDS             57539..57814
                     /locus_tag="IWO35_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ0315285.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            57820..58587
                     /locus_tag="IWO35_000199"
     CDS             57820..58587
                     /locus_tag="IWO35_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ0315286.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            58755..60584
                     /gene="typA"
                     /locus_tag="IWO35_000200"
     CDS             58755..60584
                     /gene="typA"
                     /locus_tag="IWO35_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ0315287.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            60845..62617
                     /gene="recQ"
                     /locus_tag="IWO35_000201"
     CDS             60845..62617
                     /gene="recQ"
                     /locus_tag="IWO35_000201"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ0315288.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            62731..63033
                     /locus_tag="IWO35_000202"
     CDS             62731..63033
                     /locus_tag="IWO35_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ0315289.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            63036..64199
                     /locus_tag="IWO35_000203"
     CDS             63036..64199
                     /locus_tag="IWO35_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0315290.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            64225..67653
                     /locus_tag="IWO35_000204"
     CDS             64225..67653
                     /locus_tag="IWO35_000204"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ0315291.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            67674..68798
                     /locus_tag="IWO35_000205"
     CDS             67674..68798
                     /locus_tag="IWO35_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315292.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            68788..69225
                     /locus_tag="IWO35_000206"
     CDS             68788..69225
                     /locus_tag="IWO35_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0315293.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            69209..69547
                     /locus_tag="IWO35_000207"
     CDS             69209..69547
                     /locus_tag="IWO35_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ0315294.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            69666..70226
                     /gene="rsmD"
                     /locus_tag="IWO35_000208"
     CDS             69666..70226
                     /gene="rsmD"
                     /locus_tag="IWO35_000208"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ0315295.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            70223..70714
                     /gene="coaD"
                     /locus_tag="IWO35_000209"
     CDS             70223..70714
                     /gene="coaD"
                     /locus_tag="IWO35_000209"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ0315296.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            70704..71747
                     /locus_tag="IWO35_000210"
     CDS             70704..71747
                     /locus_tag="IWO35_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0315297.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            71906..72634
                     /locus_tag="IWO35_000211"
     CDS             71906..72634
                     /locus_tag="IWO35_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ0315298.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            72750..72995
                     /locus_tag="IWO35_000212"
     CDS             72750..72995
                     /locus_tag="IWO35_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0315299.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            72992..73210
                     /locus_tag="IWO35_000213"
     CDS             72992..73210
                     /locus_tag="IWO35_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ0315300.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            73346..73885
                     /locus_tag="IWO35_000214"
     CDS             73346..73885
                     /locus_tag="IWO35_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ0315301.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            73878..76151
                     /locus_tag="IWO35_000215"
     CDS             73878..76151
                     /locus_tag="IWO35_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ0315302.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            76189..77235
                     /gene="holA"
                     /locus_tag="IWO35_000216"
     CDS             76189..77235
                     /gene="holA"
                     /locus_tag="IWO35_000216"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ0315303.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(77324..77578)
                     /locus_tag="IWO35_000217"
     CDS             complement(77324..77578)
                     /locus_tag="IWO35_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ0315304.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(77978..78247)
                     /locus_tag="IWO35_000218"
     CDS             complement(77978..78247)
                     /locus_tag="IWO35_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315305.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(78717..79172)
                     /locus_tag="IWO35_000219"
     CDS             complement(78717..79172)
                     /locus_tag="IWO35_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ0315306.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(79310..81238)
                     /locus_tag="IWO35_000220"
     CDS             complement(79310..81238)
                     /locus_tag="IWO35_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ0315307.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(81435..82268)
                     /locus_tag="IWO35_000221"
     CDS             complement(81435..82268)
                     /locus_tag="IWO35_000221"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ0315308.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(82460..83164)
                     /locus_tag="IWO35_000222"
     CDS             complement(82460..83164)
                     /locus_tag="IWO35_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0315309.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(83187..83813)
                     /locus_tag="IWO35_000223"
     CDS             complement(83187..83813)
                     /locus_tag="IWO35_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0315310.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            84139..86514
                     /locus_tag="IWO35_000224"
     CDS             84139..86514
                     /locus_tag="IWO35_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ0315311.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(86538..87029)
                     /locus_tag="IWO35_000225"
     CDS             complement(86538..87029)
                     /locus_tag="IWO35_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ0315312.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            87216..88136
                     /locus_tag="IWO35_000226"
     CDS             87216..88136
                     /locus_tag="IWO35_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0315313.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            88290..88634
                     /locus_tag="IWO35_000227"
     CDS             88290..88634
                     /locus_tag="IWO35_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ0315314.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(88824..89324)
                     /locus_tag="IWO35_000228"
     CDS             complement(88824..89324)
                     /locus_tag="IWO35_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0315315.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            89852..89935
                     /locus_tag="IWO35_000229"
     tRNA            89852..89935
                     /locus_tag="IWO35_000229"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:89886..89888,aa:Leu,seq:cag)
BASE COUNT        29313 a        15532 c        19270 g        26134 t
ORIGIN      
        1 attgacagta atgggggaaa ccggttggag agcaaccacc ttgaactatc agtggccagt
       61 agcatttagt ttactaactt tttatccgtt ttttcaactc ttaagaggag aagaaataaa
      121 tagaaaaata tattgggtaa gtattccttt attgatattt ttaactaatc aagaacaagt
      181 caacgcctgt ttttttgttc taacaagtat agttagttta tatttgattg tcaatggacg
      241 atataattat aagttatcag tattttcaat aataagctta gcagaattaa ttttctcttt
      301 gacaactcca gggaatgcat tacgtgctgc acatgaaatt aataaatggt ttcctgaata
      361 taaaaatttc aactttctta ataagttaga tttaggtatt tcatcatttg gaaagccgtt
      421 ttttttagac atgaatatct tatttttatt gctatttttc ttaattttcc ttctaacata
      481 taggaaatgt caaaactatt atgtgcgtat attgactgca ttaccgtttt tcttaaacct
      541 gattatttat tttggtaata caatgggtca aagctttact tacgtcaatg gaaataaaag
      601 ggcaatgatt tggagtagta gtaacctgaa taatctcttt acagagttag gaacgaaact
      661 gtcacttttt tatccaggta cttggatagc tactttagta gtcttggcat tattactatg
      721 tttaattgtt ggaatttatt taagttttga caataagaaa acttctattt ttttagttat
      781 tttgatgata atgggatttt gttcaaggtt aattatggga ttttctccca cagtatgggc
      841 ttccggaatg cggacgtact atatacttta tgttgttatc gccatcttag tactaatggc
      901 agttaaagag ctaatgaaaa gcatgagtgt acaaaaaaat gaatttatgc aatttggatt
      961 aaccgtgctt ggtatttgta cctttataat aacggtgata aacagataat atacaaaaaa
     1021 tccctcgctt tagtattagg tgagggattt tactcgtttt tcgattaatt gttttaacac
     1081 ttgcaagttt ctacgagtag cttgtttccg accttcatat actcacctca ggctgtttac
     1141 atgattgtac gttctttttg taaaggatac agtgatatgt atataatggc ataaagaaac
     1201 gttcaaacaa aaaaactcgt gaagcaaaat taacttcacg agttgtagtg cagatgatgg
     1261 gactcgaacc cacacgagct aacgctcaca tgaccctcaa tcatgcttgt ctgccaattc
     1321 caacacatct gcgtaataat aacacactaa tgatagcgag gttttacata gttgtcaagc
     1381 tcaaaaatgg gaggacttat taagatggag aaatatgacg gagaattttc aggactggga
     1441 atgatcttag gtgtattgat tggacttgca ttcggacgtt ttttgcttgg ttttatgcta
     1501 ggaatcattt gtggaattgc aatggattgg gcagcgaatc tatggaatga ctatcatgac
     1561 aattaagttc tcaacgcttg tttgtgtttt tttaatactt acgtttttcc taaataagtg
     1621 gcaaaatttt ctaggatgga gtatgatatt gaaatagtaa ataacaaaaa atttggaaag
     1681 ttctaacttt ttgaaatgag ggattggcta agtgatgaga gaatttgaaa aatccaacaa
     1741 attagaaggt gtcagttacg atgttcgcgg tcccgttcta gaagaagcgg agcggatgca
     1801 agaagagggc attagtattt taaaattgaa cacgggaaat cccgcacctt ttggatttga
     1861 agcaccaaac gaagtcattc gtgatttgat catgaatgcc cgtgactcag aaggctactc
     1921 agattccaag gggatctttt ctgctagaaa agcaattgag caatattgcc aactgaaaca
     1981 ttttccgaat gtgacgatca atgatatcta caccggcaac ggagtgagcg aattgatcac
     2041 gatgtgtatg caggggttgc tggacaatgg agatgaagta ctggttccaa tgccggacta
     2101 tccgttgtgg acggcttcta ttgcattagc tggaggcaca cctgttcatt atatttgcga
     2161 tgaagaggca gaatggtatc ctgatattga tgatatcaaa tcaaaaataa cgagtcggac
     2221 aaaagctatt gttatcatta atcctaataa tcctactggg gcactttatc caaaagaatt
     2281 attagaagaa atcgttgaag tagctcgaca aaataacctg attatttatt ccgacgaaat
     2341 ctatgatcgc ttagtgatgg atggtctaga acatattccg attgctacat tagcgcctga
     2401 tttgttcgtt gttacgctga acggtctatc caaatctcat cgtgtagcag gatttcgagt
     2461 aggctggatg gtcctaagtg gagataagtc acacgtcaaa ggctatatag aagggctgaa
     2521 tatgttgtcc tccatgcgtt tgtgttccaa tgtattgtct cagcaaatta tccaaaccgc
     2581 gctaggcggt tatcaaagtg tcgatgagct gctcctgcca ggcggaagga tctatgaaca
     2641 aagagaatat atctataatg caatcaatga tattcctggt ctttcagccg taaaaccaaa
     2701 agctgctttt tatatattcc caaaaattga tacaaagcga ttcaatattt tggatgatga
     2761 aaaattcgtt ttggactttt tacatgaaca tcatattcta cttgtacatg gtggtggatt
     2821 taattggaac cagccagatc attttcgaat cgtttatctt cctaaaatgg atgatttgaa
     2881 acaaacagct aagaaaatga gagaattcct agcaacctat cgccaaaaat gattttaaaa
     2941 accaaatttt tcagtaggag ggccacagac aaacacactg aggctctctt tctagtggag
     3001 ttttcttaaa taactaaagt atattattta gttgcaagtc ttttcttgaa gtttgcgact
     3061 tcctaaagta aaatacgaat agaatgcgaa tacgcatatt ttggggagga tacttatgga
     3121 aacgttaatc gagtcctttg gaacgatcat taaagagatt cgaaaagaac aaaaaatgac
     3181 gcaaaaaatg ctttcgcatg atatttgttc acaaagtgta ttaagtcgga tcgaaaataa
     3241 tgaggaactg ccgaatgtat tggtgatgat gcagatttgt caacggctag gggtaacgat
     3301 tgatcatgtg atgaatctct ctcgaaaaac tactcggaca aacgataaat tatttgaact
     3361 gttggatagt tgtttccaaa aacgtgatta tcggaagtta gaacaagtac taaaatcttc
     3421 tgatatttct gaaaatctgt atcaagcaac tgactttcaa cgctattatt attatctcgg
     3481 tgtttgcgaa ttcacgttgc gtcaaaatat ctctcagtcc ctttattatt tgaaagaagc
     3541 attaagctac tctagccaaa aagaaagaat ccatgtttct gacacagaaa tacaactgat
     3601 tagttgtatc ggaaaaatat atggtgtggc aggaaaaacg gctgaagcac gctactattt
     3661 gagtcgaagc atccaactgc tgcatcaatc agctgatgaa cgatcaaaaa gtgaattttc
     3721 gcaaattttt tataattatg gaaatttctt attccatcaa aatgaaatcg acgaagcgtt
     3781 agaacaggtg aatcaaggaa tctattgggc tcaagagaag aatagctact attacctcaa
     3841 tgatttattt gtgcttaaaa gcttaatata caagcgtaaa gaagaaccag aaagagcagc
     3901 attctatgaa ggcttagcac aagctgtgaa acaaatttca aaaaatttgt aaatggtgta
     3961 gaccattctt gacttcttct ctaaataaga gtactttaga ggtagaaaaa caaagggaga
     4021 gaatagaatg agaactttta tctatgctga caaattcttt ttagcgtcag gtgtcaaaca
     4081 tgcaggctat cttgaaatta ccgatggact ttttggtgaa tacaccaaag aactacctga
     4141 gggagaagtc aacatcattg accaaagcgg caaatggatt gctcctggtt tggtagatac
     4201 acatattcac ggctatatga atcatgatgt aatggataat gatgcagaag gcatcaaagt
     4261 gatgtctgaa ggtctgttat cttgtggagt gacttcattt ttaccaacaa cattgacgtc
     4321 aagcaaagaa cgtttaaaag atgtagctga gacaatcggc aaaatgtatc aggaagcacc
     4381 aggagctaaa atcaaaggaa tctattttga aggtcctttc ttcacagaag aacacaaagg
     4441 ggcacaaaac ccaagttatt tcggggatcc tgatttggat actttccatg aatggcaaga
     4501 agcttcaggt ggtttaatca aaaaaattgc tttagcaccg gaacgtcaag gtgtagaaga
     4561 gtttgtcaaa caagtgactg aagaaggagt agttgtttct ctaggacaca gtaatgctac
     4621 actagaagaa gcgcaaaaag ctgttgaagc aggcgcaagt gtatttgtcc atgctttcaa
     4681 tggtatgcgt ggattgaatc atcgcgaacc aggtatggtg ggtgcgttat tatctttaga
     4741 acatgtcttt tctgaattga tttgtgatgg ccatcatgta catccaaatg ctgcgggaat
     4801 cctaatggaa aaagcaggac atgatcatgt cgcattaatt acagactgta tgatggccgg
     4861 tgggatgcct gatggaaatt acaatttagg cgaatttcca gtagtagtaa aagaaggaac
     4921 agcacgtcta gaatctggca acttagctgg aagtatattg aaattgaaag aagcaattaa
     4981 aaatgttgta gactggggat tagcaacacc agaacaagca gtcatgatgg ctagtctgat
     5041 tccagcaatc agctgtaaaa tcgatgatac atgtggaaaa atcgctcaag gacgcgatgc
     5101 agactttatc gtattgaacc ctgatatgac attggaagca acttatctag atggcgtgga
     5161 acgtttccac gcataatata atttaagtta accttaaaac taaacttctt aagtttggtt
     5221 ttaaggtttt tttgtgttct agtaaaaatc actttacata agtttcaaaa gtttctttaa
     5281 tttcctattg ttcaataagt aacaatgtaa agataaaatt aaaaatgtta atacgtatta
     5341 gcaaaataaa agcttttttt attgtgaata aatgaacata attaggagga gaaagatgaa
     5401 cttatttgac atttttcgat cgactttgac agatatgtcg attgtcagtg caatcacttc
     5461 aacagttttt attatattat tagggtttgt ttgtagaaaa aaggggattt tttctgaatc
     5521 tgttggaaaa attctatcga aagtcgtctt aacagtggct ttgccagcac tggcatttcg
     5581 agctttcatg caagatatca atacagaatt actgatgcaa ggaatcaatg tcttgatttg
     5641 gggattagtt atttatatta ttcttattct agtttcaaaa ccttttttct taagctataa
     5701 aggggataag caaacaacgt tgcgtgtcct gacgatcttt ggttctacga cctttttcgg
     5761 aacgcctatc gtcagtgcga tttatggtcc tgtcggcgtg atgtattcct caattttcaa
     5821 tattggctac cgaatctttt tgtattctta tggatacata aaaatgagtg gcttgaagat
     5881 ggaagctaaa aatatcaaag cgatgttcgt caatccaatc attatcgcta cttttgccgg
     5941 ccttttcatt tggctgttcc aagggtatct gccacaagtg tcagttacag gtgcagaagg
     6001 agagataagc caagtagcgt ttttgcgaat tgaccagact gcaccgtggc tatttcagcc
     6061 gatggattat ttagctggtt taagttcacc attggcgtgg ctagcaatcg gcgcaacatt
     6121 aggtgaagta agcttcaaaa aagctgcatc tgaaaaaact tcatggtatt acagtctagt
     6181 caaagtggta tttgtacctg cattgaatat cgtgattcta gcagttttga cggcaacgaa
     6241 tattctacca gtcaattttg aagcactggc aacaatcgtt atcatgatgg ctacaccagc
     6301 tgcaacagtg gcagcagcct atgctatcag ttttgatagg gaggcacttt tagcctccaa
     6361 tgcatcctta ctgtcaacta tttttgctgt ggtactgaca cctgtatgga tcgttgtatt
     6421 acaaatgatt catggcatag gtttattttc ataagaaatg gaggaagaaa gtatgagaat
     6481 cgcatgttac ggtgttcgtc cgaacgagat agattttttt gaaaaattga atatttacca
     6541 ttatgatctc agtctttatg aagaactgtt gacacatgac aatatcgtgt cagcaaagga
     6601 ccatgatgct gtattgttga ggggaaattg tatagcagat gagaccaatc ttgcaaaaat
     6661 gcaggaatat gggattcgtt atgtttttac acggactgtg ggagtcaatc atatcgatct
     6721 acaagcggca gctgattttg gtatgacagt agcccgtgta ccatcgtatt cgccaaatgc
     6781 gatagcagag ttatctttga cttttgccat gatgcttttg agaaatactg cgtatacgac
     6841 gatccgaaca tctttcaaag attttcgagt ggatgagcaa atgtttagtc gtgaaatcag
     6901 aaattgcaca gtagggatca taggaacagg caggataggg ttgacggaag ctgttttgtt
     6961 taagggacta ggagccaaag tgatcggcta cgatattcat cagacagaag ctgctaaaaa
     7021 agtattgacg ttccactcgc ttgaagagtt attagccaaa agcgatattg tcagtattca
     7081 tgtacctcat atcccaggtg aaaacgatca aatgatcaat gaaggatttt tagctcaaat
     7141 gaaaaaaggt agcatcttga ttaatacagc ccgtggagag cttcaagata atgaagctat
     7201 tttaagagct ttgaaaagca atcatttagc tggttttgca accgatgttt ttgcaaatga
     7261 aacagaggtt tttttccgat ccttcaagcc atgggagacg attccagacc ctgctatcca
     7321 gcaattagta gaaatgtacc ctagagttct tgtaaccccg catgtaggtt caaatacaga
     7381 tgaggcatta tcaaatatga tttcgacaag tttcgagaac tttcgtgaaa taatagaaac
     7441 tggaaaaacg aaaaatgagg tatctcttcc taaagcccgt caattaagat aagaaaacat
     7501 ggatattcgt caataaaatg tttaaaaata gtccaagtaa acagttgtta cttctgtata
     7561 cttggactat tttgtatggg ataattcgat gactcaacag taatctgtga gctttttccg
     7621 cccattcttt gtcaattcat aacaggttag cggctggccg atttccaaat atttgattct
     7681 ttcgttcaat atatcttgtt ctactaaaaa attcaaatat ttcttgacgg atatacgtga
     7741 cagtgagctt ttttgagcca gttcttttgt tgaaaaaata gtatcttctt ttagaataaa
     7801 tgcacttatt ttttttaaag tcagttttga cagaccttta ggcaaatcga tgaatgatgg
     7861 tatttcgtga atgggagtgg tgtggttgaa tacttgatcg atcaatttct gatcggcttt
     7921 ttcagtcgtt tggattgtct gagagaaagc aagatacttt tcgatcgcca gctgaaaacg
     7981 ttgcaaagtg aacggtttga tcaaataatc taaggcaccg tagcggatca cattctttat
     8041 ggtttcgata tcatctgcag cagtgatcaa aatcactggg atatcatttc cttgctgtct
     8101 aagccaagat aaaaaatgga taccactttc gccaggcatg tatacatcaa gcagcagcag
     8161 atctgcgttt ttttgtctga aatatttttt tccttcttct acatttctga aactttcgat
     8221 tacgttcata ttagggatgc tttccacaaa agtacgattc aattctgcaa ccattggatc
     8281 atcttcgatt atcaggacat ttgtcatcat tttgatcttc cttttttggt aattctagat
     8341 agacgattgt accgccattt gtttgtttgg aaaattctaa aaatccaccg tggcgtttga
     8401 cgattgaatg gatgagattc aatccaaacc cacgattgat tcccttcgtc gaaaaaccta
     8461 gcgtaaatat tttttctgag atttcttctg atatcccgac accgccatcg cagacggtca
     8521 cgatgaatac ttcagcttct gtgtcatagt ttaaatgaat ccggacctgt ccgtttcttt
     8581 catgtttttg tagcacggct tcttttgcat tatcaagtaa gttccctaac acttgcaaaa
     8641 gtgaatggat ggagtcatcc atctccattt tagggataaa agaatcgctt tctaacagca
     8701 tttcaacaga ttgttctttg gcttcgttta ttttgcctaa taaaaaaccg gcaattgcag
     8761 gtactttgat cagacttgag acttggccaa tctctttttt ataattttga tttagctgct
     8821 ggataaaatg ggtgacttct tcatacttct tcaaatcaat caaaccagaa atgacatgaa
     8881 gtttattcat gaattcatgg gtttgagctc tcatggagtt tatatattgt ttcgtccccc
     8941 caagctcctc cattaatttc attacttctg atcgatttcg taaagttgcg actgctccta
     9001 aaaaacggtc attgctaaaa agaggtgtaa cgctagaaat agtttctatc ccatttaaat
     9061 acatggattg agtccgaacg gtcgtttgat gctcaatcgt ctctttaaac aaaacgtcat
     9121 atagattatc actgattttt ttcctacttc gaaatccaat agctgctctg tttggtagaa
     9181 aatgtttttt gcaggtgtat tgatcaagag gatagtttta tcagaagaga tggctaagat
     9241 tccttcatct acttcatttt cgataatcgt tttttcactt aactgttctg ctatttcttg
     9301 tggttcaagt ccaagcagta cttttttcat tttttgggca agaaaaacgg cgcctatgat
     9361 gcctacgcta agtccaataa tcaatgaaaa aacaatcatt ttttgtccat ctttgacttg
     9421 attgtcaatc gtgttgtttg ttaagccaac acaaactgca ccaataatct cttgatcata
     9481 ataaataggg acgaaatagc ggaagccgaa ccctaacatt ccttcttttt cagagaaaaa
     9541 ttcttgtcct ccaatcactt ttttcacatc ttcatgactt gagaaaggaa gaccgatgta
     9601 acttgggtct gcgtgagaat atcgaatgcc ttcagggtcc atgacaacga taaaatctaa
     9661 attcgatgca atcatgattt catttgttat tccttgaatc gcatgtgtcg ggtcaccagt
     9721 ctgcagcttt ttttggatga gtttatcatt ggcaacgatt ttagcaatgc tagagatttt
     9781 atctgacaca ctagatcggt tcatcgaaag aataaaattc ctgatcaata tggcagaaac
     9841 aatcaaagaa acgaatgtgg agaaacaaac gacaaggatg atcgtgttct ggagctttaa
     9901 tggttttttc ttaaaaaaag gaaacatagg ctacctcgaa agaatttatt gtgaaaattt
     9961 taacatgaaa acaaaaattt aacaaattgt caacgataaa cacaaccttc ctataaaaaa
    10021 caccactgag tcggtaaaag aagctataca gaattttcaa acattggtat gatagacgta
    10081 gaaatggaag gaggcgtgta tatgataggg ggaatcgcac ttgagacgat ttattggtat
    10141 acattgctga tttgtgcagg attggcagtc ctattgatta tttttggaga tgtattcgat
    10201 tttgatggcc cgcttgatcc gatgcttatt atcccttggt taactttcac aagcttattt
    10261 ggttttctag gtgagcgttt aacagatcaa tcatctttat ttttatttat tgctagtgga
    10321 ggcatatcga tagtgttggt atttttgctt aatttctata tattgatgcc gatgaaacat
    10381 gccgaatcaa cgatgtcgtc ttctgaaaaa actctggaag gacaagtagc tgttgtgatc
    10441 acaccgatcc ctgtacgagg tatgggagaa attcagttga aaagtgttac cggttccatt
    10501 agtcgtccag cttgttttta cagcccccaa gaacagacag ctgagcgggg aacgaaagta
    10561 ttgatcattg agatcaagga aagaatctgt tatgtcatgc cttatgaaag catgataaaa
    10621 gcttaaagga gcgtgtgtta aatggaaata ccagttgttt ttatcgcgat cgcaatcgca
    10681 gctttgattt tactgatgtt gctgatcgtc tttgtagcga agtaccagac agcaaaaccg
    10741 gatgaagcgt tgatcatcag tggaagttat ttaggaagca aaaatgtgca cattgatgaa
    10801 agcaataaca aaatcaaaat tgtgcgtgga ggtggcgcat tcgtcttgcc agtttttcaa
    10861 cgttcgaatc ggatcagtct attatcgagt aaattggacg tctccacccc tgaagtgtat
    10921 acggaacaag gcgtaccggt gatgtgtgat ggtacttcga tcatcaaaat cggttcatct
    10981 gtagaagaga ttgctacagc agcagaacag tttttaggca agacaagaga agaactggaa
    11041 aatgaagcaa gagaagtatt agaaggacac ttgcgctcga ttcttggttc tatgactgta
    11101 gaagaaatct atcaaaatcg cgataagttc agtcaaagcg tacaagaagt cgcttctgta
    11161 gaccttgcaa aaatggggtt gatcatcgtt tctttcacta tcaaagaggt cagagacaaa
    11221 aacggttatc ttgactcttt agggaaacca agaatcgccc aagtcaaaag agatgcagat
    11281 atagcagaag cggaagctct aaaagaaacg cgtatcaaaa aagctcaagc agaaaaagaa
    11341 tcacaagcag ctgaattaca gcgacaaaca gagatcgcag aatcattaaa agaaaaagaa
    11401 ctgaaattag caacctacaa acaagaacaa gatgttgcta aagcaaaagc agaccaagcc
    11461 tacaatttag aaagtgcccg agcacaacaa caagtaatcg aacaggaaat gcagattaaa
    11521 atcgtagaac gccaaaaaca aatcgaactg gaagaaaaag aaatcacacg tcgtgaaaag
    11581 caatatgatt cggaagtgaa gaaaaaagca gatgccgatc ggtacgctaa agaacaagaa
    11641 gcacaagctc aaaaagtcaa agaagtgact gaagccgaag cagaacgatt ccgtgtggaa
    11701 gcactggcag aagcggaagc caataagaca cgtttagctg gacaagcaga agccgaagca
    11761 gcactagcaa aaggaaaagc agaagccgaa gcaaaacaaa agatcgccaa tgccttcaag
    11821 gaatatggcg aagcagctgt cttgagtatg gttatcgata tgctaccgca attgatgcgc
    11881 gaagcagcac agccgctagg aaatatcgaa aaaatctctg ttgtcgatac aggaagcagt
    11941 tcaggagaaa ccagtggagc gaaccgagtg acaaattatg ccacaaattt gctttcttct
    12001 actcaagaaa cactaaaagc gacaacagga cttgacttga aggaaatcgt agagaatttg
    12061 tcgacacgtg ggactactag ccaagtaaat atgtatgaag cgccaaaaga ggcagaacaa
    12121 aactcgatga aggaagaata aagtagtaca cttcaccgtt caatcaaaaa tggaggaaga
    12181 ctttttaaag ttcttctctc cattttttta ttttattgca aaagaaaatt agtcctcgct
    12241 atttttcttt tgtttttgga tcttagcttc tatctcttgt aaatttgttc ttagcaatcg
    12301 ttctttcttt tggagttcga ccaaatcttc tgatagttct tccttctttt ctgtttcagg
    12361 aggacgaaga aagaaattga taccgattgc aatgaatgtc cctataaaag tatctagtac
    12421 acgttggata gcatacaaag cggattcgcc ttgagggatg ctcaacgcaa tcagcaacaa
    12481 cgtagctatt gcggaaatga taccggaatt attgttaata ccatctgaca cgacgatcac
    12541 aataataact agaataggaa gcaagatcaa ttctaccaaa aaatcattgt gaaaatagct
    12601 tttgacaaaa aaataaatga tagctaatgt accacctaat gtattgccga tgatcctaga
    12661 ctttccaaaa gaaacggttg tagtcaagtc ctgacgcaaa gaaaaaacag cagataatgc
    12721 agcaatcatc gggattcctc gttgagtcaa atgaaaaaac aagatacata aaaaaacagc
    12781 taaggcactt ttgatcgtac gcatacctag tcgaaaacgg ccaaattgca tagagattct
    12841 cctttttggt ctttttacta tcatacctaa acttttgtct gatagctagt ttttcattat
    12901 tcaagtataa ttaaaataaa aaagggggaa gtaaatgcgt ataggaatgc gaacagtcaa
    12961 aacagtgatc agtgtcttct tttcgatggt cacagcgggt agcttatcgc tgttgtattg
    13021 gccagctgca gggatcattg cattattaag tgtaggaaat accaagcgct ctacattgcg
    13081 tacgggcatg aatcgaatcg cagcctttat attagctacg gcaacagcac tgttcagttt
    13141 ttttgctgtt gggtatacta tttttggctt cagtcttttt ttattcctgt tcatcccgct
    13201 tgcctctcgc ttcaaattaa ccgaagggat agtagtaaat tcggttttgg tcactcatta
    13261 tttagtggag gaaaacatgt cttggcagct gatcggcaat gaagcgggat taatggcgat
    13321 tggattagta tttgctttgc ttgcaaatgt ttacatgcca gatcgtacga agcagctgaa
    13381 ggaaaaccaa atacagatcg aaaaagaatt tcgaaatatt ttgagacaaa tggcagaatt
    13441 tcttttatca gaaaataaag aagatgtgca gatcaaatgc gaacatttac tgacattcat
    13501 tcgcgaaagc caagaagatg ccagagaaca tcaagaaaac tactggctca gacagccgct
    13561 atactacgaa acttacttct ctatgagaag agcacaggcc aatgtgatta aagacatgct
    13621 ggaaaatcta gagagaatcc agcaaccagc ttattatgga aaacatatct atggtttatt
    13681 gatttatact gcagaaacat tttcagagtc aaatgatggc agacaaattt taacacgtat
    13741 tgaagaagtt tatgtattgt atcggcaaat gcctctgccg actagtcgcc ctgaattcga
    13801 ggatcgagca gaattgttcc agtttttgca gtcttttaaa agttttattg aaatcaaggc
    13861 agaattctcc caacaaaaaa tccaaaaata gaggttgtga attcagctaa tattcaggtc
    13921 aaaaagctga tttcacaacc tcttctttaa acggtgttct cctgtcaact cctcggtcac
    13981 aagtcacaat attcaacaaa catgagaagc tgttttttga aattgctcct tgtgactcgg
    14041 agctgaacgg taggctcaca acctctttct taaacggtgt tcaaaagctt tcctctgcgg
    14101 taataagtcc agccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac
    14161 ttgatgcaga acagcttttt cacaaccttt taaaattaca tatttttcca tagaacttgc
    14221 atttgatcca agatttcgat gacatctttt gtcaagaaga gagagcgatt gggcaagtca
    14281 ccttcaatca tgtgtaccat attctttatt tcgtaattca ttgcttcaga tgtattcccg
    14341 ctttcgatga attcctttgt accatctgta aataagatct ccgcacgatc agcacgcggg
    14401 taatcagcaa tcgtcacata accgttctca aaggcaacga taccgacttt tggcattttt
    14461 gcctgaaaag tcaagctgac agcagcaagt tcattttctt tgttacgcaa gatagtgacg
    14521 gattgttcgt ccacgcctgt ttcaaaagga accattgtag aggcaactac ttcaggttga
    14581 gagctcaaaa agaaacgagc gaaagataca gcatacgtac cgatatccaa cagagctcct
    14641 ccagctaatt ctggattgaa gaaccggttt ttagggtctg gttctttgta actgccgaaa
    14701 ggtgcttgga tcatttttaa agcccccagt tttcctgtat ccattattga gcgtaactgc
    14761 tgataaagtg gcatgttgaa gattgtcatc gcttctgcta agatcaaatt cttttcttct
    14821 gctaagcgca tagcgtcagc cagttctttt ttattcatcg tgatggcttt ttcacataga
    14881 acgtgtttgc ctgcttccaa tgctgccaag atatggtcga tatgctgtct atttggtaca
    14941 gcaatgtaca cgatgtcgat ttcagcatcg tctaacattt cttgatagct tccgtaagct
    15001 tttggaatat tgtaacggtg agcgaagttt tccgcttttt ctgaagttct ggaagcaact
    15061 gctgttagtt cgctcgtctc ttgattgaag ctttcggcaa actcatgtgc gatggtacca
    15121 agaccaacga ttccccattg atattttttc atctgaaaac gctccttcat agttggattg
    15181 agtttcttgt ctgtctatta ttataacaaa aatcacgaag aataaagagg agaggacttg
    15241 aaacattaaa aaaaatgaaa gcagcgattt aagaggtgct ttttgatcaa aacttaagta
    15301 gaatagttaa aaggagggga taaacgtgca tcaagtatat attgatctta ttatgagtgt
    15361 attattagaa cagtttgaaa ctgaaaaaaa ttttttagat gaatacttgc tgatcagtcc
    15421 agaacaatgg caaaaatgga aagatggcga gaaaaatctg ccatctgaag cgatgcagaa
    15481 agtaaaaaat cttttcactg attatgaatg gatgctaacg caaaagattt tgcgtcaaac
    15541 gattattttt cccgaaaaac gaaacgttgc cgttgcagag tacaagcaaa tcaaaacacg
    15601 catcgcgcaa aaatggctta gcagcaaagc agcaaaagtc gagctgatcc ctttgaaaaa
    15661 taatcagcag gctggtagat accttgactt gaaagtaagt attcaatatg atgaatgggg
    15721 ctatgatgat attttgaatt ttcgtctgcc agcagatgtt caaaaacaga tcgaaggaga
    15781 aaaaatcgaa cttctcgatt gggtgaatga aaatctggaa gaaacttacg ttcatgaggc
    15841 gaatggggaa cgtgaagaac aacgaaagga tttttcaagt gaataaattt ttaggatttg
    15901 gagcagttgt gattatgatc gcagcaatgg gctatgttat ttttagcgat gatgattcgc
    15961 atgttcaagc cggcaatcaa gcagtcgaac gaacaagcac caaaacaact gctagtaaag
    16021 aaaagaacag tcaaacaacc agcagcaagg taaaggacaa agaaacaaaa gatcgtacgt
    16081 ccaaacaatt gccagatgtt aaaacagacg attggaagct agttttggtt ggtccagatc
    16141 acaaattaga aaaagaaatt gatgagtcta ctcaattggc atcattaagt aacggatact
    16201 tgatcgacaa acgcattgct ccagaatatg aagcatttgc caaggctgct gaagcagctg
    16261 gatttccatt agtgatggtc tcagcttacc ggtctgtatc atcccaacaa caagttttcg
    16321 aacaaaatgt ccaagatgtg atgagtcgtc aaggtgtatc agaagaggag gcgacaaaga
    16381 ttactaaaga aacgattacg gagccaggat acagcgaaca ccacacaggt ctggcagtcg
    16441 atgtcgtcga tcaaaactgg tacaacagtt atccttctac tgtattggat gcaagttacg
    16501 gagatcagcc tggagctaaa tggatcgcag ataatgctgc gcagtatgga tttatcgttc
    16561 gttatccaaa aggacacgaa gatataacta agatcacata tgaaccttgg cacttacgtt
    16621 atgtcggtaa agaaaacgct gagtatatca ccaaacacca attgacgcta gaagaatatc
    16681 tgaatgaatt aaatgaaaag taggaattga tcatggcgaa aaaaaagtgg aaaaagcaaa
    16741 agcgcaagaa tatccgttgg ccagcagtgg ttgccggtgt agcagtgata ctcttagcct
    16801 ttgttttttc cttgaagaat ctgtcttctc cttttactga taatcagcaa gaaaatgaag
    16861 cgcaatccca tcagcaattt attgaacggc ttgttccgca tgcccaagaa ctacaagatg
    16921 gttatggagt gcttcctagt atcattttag gacaagctat attagaatca aattggggaa
    16981 aaagtacctt ggctagcaaa tacaataatc ttttcgggat caaggcttat ggggatcaga
    17041 agaaagtaag cctggaaaca aaggaatttg ttaacgaaga atggataact atccaaggag
    17101 acttcaaagt gtatgattca tgggaacagt ccatggatga ccatacacaa ctgttcgtcc
    17161 aaggagtaga ttggaatcct gcgctgtatg aaaaagtgat tacggccacc aactatcagg
    17221 aagccgcaca agcattgcaa gatgcaggtt atgcgacaga ccccggttat gcgcaaaaaa
    17281 tcatccaggt catcgagacg tatcaattga atcagtatga ccggagatag caaaggagaa
    17341 gtggctgatg gcagaacaat ttacgccaga aatcatgacg gaattgagaa aagatatcgt
    17401 aagagtaccg gaaatcattt tagaagcaag cgggatcaag atatttggaa aattgatccg
    17461 ttcgatcatc tttactacgg atattgcgat catacgtaat acaaatgcga atgcagtaat
    17521 tgctgtgtat ccttttacac ctcatccagc gattactaaa agcattattg aggcagctga
    17581 cattccagtt ttctccggtg taggtggtgg tttgacacaa ggtgctcgtt cttcctacat
    17641 gagcatgttc gcagaagcgc aaggttcgtt aggggtcgtt ttgaacggtc ctacgccttt
    17701 agagacagta aaagaagttt gtcgagtagt tgatattcca gttatcagta cggtaacgtc
    17761 gaaatataca gaaatagatg aaaagttagc agctggtatt tcgattatca atatcagtgc
    17821 aggtaaagaa actgcagaag cagtacgcca ttttcgtaaa aaatatcctg aacttcctat
    17881 cattgcgaca ggtggtccaa cggaagaaag tattgctgag accattcaag caggagcaaa
    17941 tgccatcaca tatacgccac caagtagcgg agagcttttt agtcgtaaaa tggaaaaata
    18001 tcgggaaact gagaaaaaga atcatgaata acaggtgaga atcaaaagac gaggaagagc
    18061 agatagtggc tcttcctcgt cttttgtact ccagtactat ttgttcagaa ttcaaaaaaa
    18121 agagaaattt ttttgttttt ttacctgtaa tgtacataag aagagttaaa accttttgtt
    18181 attattagca ttctaacaaa aaaatgtctt gatttattga agcgctttct atagaatgaa
    18241 aaacgaataa aaactaaaaa aggcacccga caatttagta gcagctaaat tgtcggggct
    18301 tgaaaagtca gcgggaactg accaatcaag ttgccatagt caatgcttgt gcatcgacgt
    18361 gtatattgta cctaatttca tacagaatta ctagttattt ttctgttttt tagggaagag
    18421 cgcacacgaa gaagcatgct tatttagcta tgtcaaggat attgggcagt tgtctgatat
    18481 ccttttttct ttgcgtaatt atcctcccat cattgaagca agaagtgttt ttagcgatga
    18541 aagaagaatc agcactatct aatttttctt gaaagtattg agttgttcat cctaggcaga
    18601 acgataaggc aagtcttggt ttttattccc cttgaaaaag atcgtttaat aaatatgttc
    18661 tgtataaaaa tacagtatat tgaaaaataa caatggaagt agaggtgcag agaatgaaga
    18721 aaagaatgag aatcatctta gctttaagtg ttatgttatt cttcttgacg gcttgtgggc
    18781 aagaagatga caaaaaaatc acgctgaaca tggcttggtg gggttcgcaa gtccgacacg
    18841 atgcaacagt aaaagtaatc gagctttatg aagaaaaaaa tccccatgtg acgatcgatt
    18901 atgaatttta tgattatgaa ggatattaca caaaattagg tacactagca gcatcagatg
    18961 atatctggga cattttccaa ctgaacaatc aatttcctca atacatcaat cagattgaac
    19021 cattagatga atatgtggag caagggataa ttgatatttc taatgcagat gaatcctatt
    19081 tagcaacgac gacttacgac gggcaattag ttggactgtc taacggggtg aattcgtttg
    19141 cgattgctta caataaaaat atatttgaac aattaaatat ccctgaacca gcttcaaatt
    19201 ggacatggga tgaatttgaa caaatcagct atcaaatcac tgaagaatta ggaatctatg
    19261 ctatttcaag attcgaagat tttatagctg gctgtattat tcaaatacca caagttgaga
    19321 aaggattgaa tttttataat cgggaagatc tatctaacag cttgggattt gaaaatccag
    19381 actatctaac agattttttc cagctgcgta aaaatctggt agacaaaggg gcccatccag
    19441 aaccaggtgg tggagctgca agtacgacgg atctgtcttt tcaggcggtt agggatagag
    19501 aagcggcaat ggtgtttcta tcaagtaacc agttgacaga aatattgtca ggggctcctg
    19561 aagatatgga gattcagttg attaatccac ctcgcagaaa agcagatgga gaatctggta
    19621 tccctttacg ttcttcgcaa atgctgagta tggcaaaaag ctcagcccat aaagaagaag
    19681 ctgctaagtt tattgatttc tttcaaaaca gtgaagaagc caatgaaatt ttaaaaggag
    19741 aacgtggtgt accaatcatg tcgaatattc gagaacagct cgctcgtgca gatgatgcaa
    19801 ctttgcaagc gacatttgat tatttagaaa tgatttccga tatggacaac ggcgaagtga
    19861 atgtctttga tagtgctttg aatccggaaa ttcaagacca atacaatctt ttagttgaaa
    19921 aagtcattta tgaagaagta acagcagaag gaaggcatga aaaaagaaaa actaaaacgg
    19981 ttcttggatc aagacaatgt ggtcggttac gtatttgctt ctccattcat cctaggattt
    20041 ctattgataa cagtgatacc gatgatcttg tcactttatt attcgttcac cgattatcgt
    20101 ttgggaaatc cagtctcatg gatcggcttg gataattata taaagatgct gagtgatgcc
    20161 cgttttttga actccatccg agtcactttt ctctatgtat ttgcatctgt acctgtgaaa
    20221 ctgatctttg gtctattgat tgcttatctt ttaacgagga atatcaaagg aacgactttc
    20281 tttcgttctt tgtattatgt gcctagtttg atcggtggca gtatttctat cgctctagtt
    20341 tggcgggagt tgttttctaa aaacgggtta gtgaatattt tcctggaacg attaggcgca
    20401 cctgcgcttt cctggtttag cgatcaaaaa ctggtcatga tccctcttgt tttgatgtct
    20461 gcttggcaat tcggttcggc aatgatcatc tttgctgcag gtatcaaaga gatcccaggc
    20521 tcgtattatg aagcggcaga gatcgatgga gcgaacaaat ggcagacgtt ttacaagatc
    20581 acgttacctc ttctttcgcc gatcattttg tataacttgg ttatgcagac aatcgcggcg
    20641 tttatgactt ttacgcaggc atttatcatt acccaaggtg gtccgaatga tgccacgaac
    20701 ttttatgctc tttatgtgta taaccaagct ttttctttta attctatggg gtatgctagt
    20761 gctatttcgt ggatcatgct gttgattatg ggagctgtca cctttctgat tttcaaatca
    20821 tcaaaaaaat gggtcgttgt gtgaatgaga ggaggaagaa atgaataata cagtaacgaa
    20881 atggtttcaa tacggtttta ttttgctatt aggtctagtc atgatttatc cggtcttatg
    20941 gatgatcgca ggttctttta aaacggatca agagattatg agtggttcgc tgaatttgat
    21001 tccagagact tttcaatggg aaaattacgc aaaaggatgg gcggggtttg ctggaatttc
    21061 attttttact ttctttagga attcgtttgt catctctgca gtttcgacga tagggacagt
    21121 tctgagctcg acttgtattg cttacgcatt atcgagaatc gatttcaaag gaaaaagatt
    21181 ctggttcgtc gtcatgttag taacgatgat gattcctgca caagtgattt tgattccgca
    21241 gtttattatt tacaatcggc tgaatttggt tgggacgtat gtgccgctga ttttaccaca
    21301 tttttttgga caagcatttt tcatttacca gatcatgcaa ttcatggtca gcatccccaa
    21361 agagttagat gaatcagcga ttattgacgg atgcagcaag tatagtgtct ttaccaaaat
    21421 tatttttcct ttgctgaaac cttcgatcat cacgacgatc atcatccagt tttattggaa
    21481 atgggacgac ttcatgggac cattgattta tttaaacaag ccgagatctt acacggtatc
    21541 gattgccatc aaattatttg cagacgctgg atctagtacg acttacgcga gtatgtttgc
    21601 gatgtctacg ttgtcgctgc taccggtttt tctgattttc ttattcttca ataaatatct
    21661 tgtccagggg atcagtacaa gcggtctgaa gggctgaata tgaaaaagcc aatagaagag
    21721 atgtttcttt acaagctcaa ctactatgtc tgtcggattt ttttaaccag tcttatcagt
    21781 aatgctcttt tcgctttagt tctaggatgt ttgttcactt taggattagt gctgtctgct
    21841 cgtgttgtct atctgattta ttttttgatg atgtatcctt cttattatct atctgtccat
    21901 tacttggcag aaaaaaaggt atgttggagg aaagggaaaa tcaaacggat gtgtttcatc
    21961 attacgttgt ttcagctgtt tgtcattatt tttgcagttg attggtattt attagtggga
    22021 aagattggag cttggtatct gattcctttt ctattgtgtc tgttgctttt tgtatcccat
    22081 agttttttta gtacgctaat tgatatctgc aaagaagata aagcagatag tcttgtttat
    22141 ttgatcaaaa ggaatatctg gctgttgtca aaaaacgtca aggggtttct gtggtattta
    22201 tgccttttgt atggactgtt ctggtgtata cagcctgatt ttttactcac tgcattactg
    22261 tatccagggc tgccatttgt cacagtattt tgcttaaaag aaaaggagaa tgactttaaa
    22321 tgaagaaaaa atatgtacaa gtcggacttg gtgggagagc gcgttttttt tatgaggcaa
    22381 ttgcctctac gtatcatcaa acttgtgaac tagtcggttt ttgtgatgta tctcaaacaa
    22441 ggatggatta tgcaaatcgg atacttgtga acaagtatgg acagaaaaaa gtaaagacat
    22501 gtatgccatc acaatttgag caactactgg atgacgtacg gccggctgaa gtcattatta
    22561 cgtcagtaga tcgaacgcat catgattata tcattcgtgc aatgaaaaaa ggatatgatg
    22621 ttatttgcga aaaacctctg actatcgatg aggaaaaagc tcaagaaata ataacggtcc
    22681 aaaaacagac gggaagaagc ctgcgagtca cttttaatta tcgttatgca ccgtatcaca
    22741 cgaagataaa ggaactgata gaaagtgatg tgattggtga tgtgttttct atccacttcg
    22801 aatggctgtt aaatacgcaa cacggggcag attatttcag aagatggcat cgaaataagc
    22861 acaatagcgg cgggcttctt gttcataaat ctacccatca ttttgatcta gtgaattttt
    22921 ggttgaacag ccgtccccaa aaggtatttg cattcggtgg attaaacttt tatgggatgc
    22981 ataatgcgga gaaacgcgga gtagaaatat tttacagtcg atgctatgga agcgaactag
    23041 ctaaagctga tccatttgct atagacctgt cagccaatga ggaattgcga gaaatgtatt
    23101 tagaagcaga aaaagacagt ggatatattc gtgaccgcag tgtattttcc gatgatatct
    23161 ctattgaaga tacactaggc gtaacggtca aatatgaaac tggtgcgatt ttgacgtatt
    23221 cgctaaacgc gtatatgccg tgggaaggat tcaatgtttc tttcaatggc agcaaaggac
    23281 ggatcgaaat gaaagtgatc gaaaagtctt acatcaatgc aggtggagat aaaaaggatg
    23341 aaggagcagc cgttcttcag tccatccatg tctatccgat gttcggtgag ccatatgagg
    23401 taatcgtcga acaagaagcg ggcggacatg gcggaggaga tccgcgttta ttagatgatc
    23461 tatttggtga agaaaaagaa gaagatccgt ggaatcgggc agcaactcac gtagatggta
    23521 ttttgtcgat tttgacagga atcgcagcaa atcattctat tgcttcagga aaagcagtag
    23581 tgattgacga gcttgtcagc ttaaaagaat aagaatagat ttacggaaat tacttcccgt
    23641 agtttataaa atcttgcctt attgccgata ggctgtcttt tgaacactat ttattcggaa
    23701 aaaggggcta tgacatgctt tttgtcatgc cttcttttta tgtgggctgc ttttttagat
    23761 aggagaaaat aaaaataact atttaaaccc gaacaaaaag gaataatgca ttaaaaatca
    23821 ttagatttta caaaaaaata agtaaaccac gttgtcagtt tcagaaagta gtgttagact
    23881 gttttcaata tttgaaacaa cattttttat tcaaatatgg caaactgcct tcacgtgcaa
    23941 aacagttgca agaaaatgaa tttacactat tattagtttt acatacctac ttatatatcg
    24001 tcataatatg gatgacagtt tctagccagt accgtaaatg ctggactata agtaaaagat
    24061 tggctatttc acccttcttt tcttatgata aagtggaggg tgtttttctt taacagaaac
    24121 atagtaggtt tttgcacaaa agcggttatt tcggtattaa taaatgataa aggagcaaat
    24181 aaagtgaaag aattagttga acgtattcaa aaagacggac gtatattagg cgagggcgta
    24241 ttaaaagtcg acagcttcgt cactcaccaa gtagaccctg tattgatgga acaaatggga
    24301 aaacggtttg cagaagtatt taaagaacaa ggaatcacga aagttgtgac cattgaagct
    24361 tctgggatcg caccagcttt atttgctgca agagaattag atgttccaat gatcttcgct
    24421 agaaaagcaa aaagtttgac gatggatgaa gaattattga cagcttctgt ttattctttt
    24481 acgaaacaag tgacaagtac gatttctatt tctcgtaaat ttttatcttc agaagacaaa
    24541 gtattgatta tcgacgattt cttggcaaac ggacaagcgg caaaaggatt gatcgaatta
    24601 tgccagcaag ctggagcgca agtagaagga atcgggatcg tcatcgaaaa gtctttccaa
    24661 gatggacgtc agttacttga agaaatggga ttgagagtcg tttctcttgc gcgtattgct
    24721 tccttgacga atggacaagt tgagtttctt gaggaggacg cataatcgtg gaaaaagaaa
    24781 cacaaaatgg aaaagctgcg gttttagggc tgcagcatct gctagcgatg tatgcaggag
    24841 cagtagcggt gcctcttttg atcggtacag gactaggctt taatcaagaa cagatgactt
    24901 atttgatttc aattgatatc tttatgtgtg gattagcgac attattgcaa ttgacagtta
    24961 accgattttt cggaattggc ttgcctgtcg ttttaggatg tgcgatccaa gcagtagctc
    25021 cactgatctt gatcggaacc aatgaaggag taggagctat ctacggatct attatcgctt
    25081 ctgggatctt tgttgtgtta gtttctggtg tcttttctaa aattaaaaaa ctattccctc
    25141 ctatagtaac aggaacggtt atcaccgtta ttggtttgac tttgattcct gtagcaatcg
    25201 agaaaatggg tggaggtaat gcaacagcag ctgattttgg tgacaaaaca aatctgctac
    25261 ttgcatttgt gacgattctc ctaattgtag gcgtgcaaat gctggcaaaa gggttcattc
    25321 gttcaatcgc ggtattgatt ggtttagtcg gaggaagcat cttagctgct ttcatgggaa
    25381 tgattgatct gagcgcgatt ggagatgcgc cgttgttcca tgttccacaa cccttttatt
    25441 ttggtaaacc aactttcgac gtatggtcga ttttactaat gatcatcatt tccatggtca
    25501 gcatggtaga atcaacaggt gtatattttg cacttggtga cattactggg aaaaatgttg
    25561 gagaagaaga attgaaaaag ggatatcgtg cagaaggact tgccgttatc ttaggcggta
    25621 ttttcaatac attcccttat actggctttt ctcaaaatgt cggattggtt caactttcgg
    25681 gaatcaaaac aagaaaaccc atctatttct ctgcattctt cttgattgtt ttaggattat
    25741 tccctaaaat cggtgcgtta gcacaaatca tccctgagcc ggtacttggt ggaggaatgt
    25801 tagtcatgtt cgggatggtg gcagtccaag gaatccgtat gctgttggaa gttgatttca
    25861 gcaatgataa aaatctattg attgttgctg tttctgttgg atttgggctg ggtttcaatg
    25921 ttacgccgac attattcagt caaatgccgc aaacattgca aatgttcact ggaaatggca
    25981 tcgtaatgag tagcattacg gctatcatat tgaatttgat ctttaatgga ttgaaaaaag
    26041 atgaagaaat attggaagaa gccttcaaga agtaatagag caaaagaaag gttgtgaaat
    26101 cagcgttttg acctgattgt tagagaaaca aacggaaaaa tagttcctcg tattttgccg
    26161 tttgtttgag ctaataccga gggtcagctg attgaacacc gtttatccat aaaaaggaat
    26221 gtgacctaac ttttgtcaca ttcctttttt tcatctttaa taaacacgaa caataaaatg
    26281 tttttctttg ttaacattcg aaatttcttt gacatcaaaa aaagtgattg ttaaaatatg
    26341 aatgtagaaa aactgggagg aaaaacaatg acagcaaaaa tctctgtgat tatggggagt
    26401 acttcagatt gggaaacaat gaaagaagca tgtcaaatat tggatgaatt cggtgtgtct
    26461 tatgaaaaaa aggtcgtttc tgctcatcga accccagatt tgatgtttga atatgcacaa
    26521 aacgctcgaa aaaaaggaat caaaatcatt attgcaggag cagggggagc cgcgcatctg
    26581 ccgggtatgg tcgcatcaaa aacaactttg ccggttatcg gtgtaccggt ccaatcacga
    26641 acattaaatg gtttggattc gcttctttcg attgtccaaa tgcctggagg agtacctgta
    26701 gctactacag caatcggaaa agcgggagca attaatgctg gactacttgc gatacagatg
    26761 ctttccatgt atgatttaga attggaggct aaattggctg aaagacgttc aatgttggct
    26821 aaaactgtta tagaaagtag tgatcaactt ggttaaacct ttgttgcctg gttcaacgat
    26881 cgggatcgtc ggtggcggtc aattaggtcg tatgatggcg attagtgcaa aagaaatggg
    26941 ctttaaagta ggtgtgctgg atcctgtgag cgattgtcca gccgcacaag ttgcagattg
    27001 gcacattgaa agtgtctatg atgacacctt tgcgctgaaa gagcttgcca gaagaacaga
    27061 tgtcattacc tatgaattcg aaaacgtcag tgtagaggcg ttaaatgcga ttttacccat
    27121 gtcatttatt cctcaaggga cagacttgct tgcgattacg caagaccgcc tgttagaaaa
    27181 atcttttttg gaaacgaata acatcgtgat tgctccttat gcaacgatcg tcagtccgac
    27241 ggatatccaa gatgcgattg acgggatcgg ctatccttgt gttttgaaaa ccactcgagg
    27301 cggctatgat ggcaaggggc agtatgtttt gaaaagtcgg gcggatctag ctcctgccat
    27361 ggacctttta agagaaggaa cctgtgtttt agaagcatgg atcccgtttg aaaaagagat
    27421 ttcgatcatg gtggcaggaa atggtcaagg agatttcacg acttttccgg tcgtggaaaa
    27481 catccaccac aacaatattc ttcatgaaac gattgctcca gccgcgattg atcaggatgt
    27541 gatcgaggag gctgagcgta ttgcccgtgt gattgcagaa gcagtgagcc tttcaggtgt
    27601 tttaggggta gaaatgtttt taacaaaaac aggcgggcta tatgtgaatg aattagctcc
    27661 tcgtcctcac aattctggac attattctat tgaagcttgt tctatgagtc agttcgatgc
    27721 ccatattcga ggtatttgtg gttggccatt aggagaaaaa gtacgtttga taagcaatgc
    27781 agtaatggtc aatgttttgg gagaacaact gctggaaagc tatcgcttga ttgagaataa
    27841 gccagactgg caattccatt tctatggaaa aaaacaagca aaaaaaggtc gcaagatggg
    27901 acatatcact atcttgacag aagacatcca gcaaacgctg gaagaaatca aacaaacgaa
    27961 tatttgggat tagaggagaa aaaacatgtt agaacgttat acacgccctg aaatggggaa
    28021 tatctggacg gaagagaaca aataccgtgc gtggctcgaa gtcgagattt tagcagatga
    28081 agcttgggca gaacttggtg aaatcccgca agaagatgtg aaaaaaattc gtgagcatgc
    28141 atcatttgat atcgatcgga tcttggaaat cgaaaaatta acacgccatg atgtcgttgc
    28201 gtttacacga gcggtttctg aaacattggg agaagaaaga aaatgggtcc attatggtct
    28261 aacaagtacg gatgtcgtag atactgctta tggctatctc atcaagcaag caaatgatat
    28321 tttacgggaa gacttgaaac gttttacgga aatcattggc gaaaaagcaa aagaacacaa
    28381 atatacagtg atgatgggtc ggacacacgg tgtccatgct gaaccaacta cttttggatt
    28441 gaaacttgcc acatggtatt cagaaatgaa acggaacatc gaacgatttg aacatgcggc
    28501 aaaaggagta gaagcaggaa aaatctcagg agcagtggga acgttcgcaa atatcccgcc
    28561 atttattgaa gagtatgtat gtaaacaatt ggggatccgt ccacaagaaa tctctactca
    28621 agttctccct cgggatctac atgctgaata tttctcagcg atggcgctta ttgctacaag
    28681 tattgaacgt tttgcaacag agatacgcgg attgcaaaaa tccgaaacac gtgaagtaga
    28741 agaatttttt gcgaaaggtc aaaaaggttc ttctgctatg ccgcacaaaa ggaatccgat
    28801 cggttcagaa aatatggctg gtctagccag agtggtgcgc ggacacatgg tcactgccta
    28861 tgaaaatgtc acattatggc atgaaagaga tatttctcat tcttcagcag aacgaatcat
    28921 cttgcctgat accacgattt tattgaatta catgttaaat cgttttggca atatcgttaa
    28981 aaatctaacg gtctttcctg aaaacatgaa gcggaatatg tctgcaacat ttggtttgat
    29041 ttatagccag cgcgttatgc tgaaattgat cgataaggga atgacaagag aagaagctta
    29101 tgatcttgtt cagccaaaaa ctgcgtacgc ttgggacaac caagtgcagt tccgcccgct
    29161 gttagaagaa gatccaacca tcacatcggt attatctaaa gaagaaatag acgatgcctt
    29221 tgattatcat catcatttga aaaacgttga cgtgatattc aaacgagtag gtttggaata
    29281 aagaagagtg agttgcaaaa ttcaattaaa atagaatagc aaaaataacc aaaaaagaag
    29341 cagatccgtt agcggttctg cttcttttag tttattatat tttcttatca ataataggaa
    29401 cctgatttta aaagcggcta cctctttacc gtgcctcttt acatacaaaa aatagaggaa
    29461 ttaagcgtaa atggcttggc tattataact aaaatgctgg ttatggagat gttgcagcaa
    29521 gttttcgaga aaagcgtatt ccatctcttg tccattgaat aagatctgga tacgataatg
    29581 atttttgaaa cgcttctgtt catgaatctg actgattgaa agttcgcctt tgtgttctgt
    29641 ctgataaaga aaagtccttt tacggcattc agcacagcgc ttggttgctc ggcggatttt
    29701 tcggaatgta taaaaactag tatctttatt gcataaataa acgtctttga tcgacagcaa
    29761 atcattatat agacaggaac gttcttcagc tgttaattct gataaaaaaa gctgaccttg
    29821 ttttattgca tcttccatcg gcaagtgcct cctcgaaccc attatccaaa tttttagtta
    29881 attctaatat accatcatta agtataaatg taaacgcttg ttgatggtat aagattttcg
    29941 tttctgataa aacacgaaca ttaaaagaat ctaatgcctt aaatgtacat atttttgttg
    30001 atttataaaa agatgattgc taaaatttaa agtgaaaaaa cgaacaaaaa acgatgtatt
    30061 tgcttttaat gtaaataaaa aggagagacg tacatggaga agaaagaact tttgtatgaa
    30121 ggaaaagcca aaaaaatata tcttacttct gatgagaact tgatttggat agaatattta
    30181 gatcaagcaa ctgcgttgaa tggcgcgagg aaagaccaga taagtggaaa aggagaattg
    30241 aataatcaaa tcacaagtgc aattttccgt tatctgactc aaagaggaat aaaaaatcat
    30301 tttattgaac aattgtctaa aaccgaacaa ttagcaaaaa aagtacaaat tatccctctg
    30361 gaagtggtta tccgaaatat tgcagcagga agtttctcaa aacgtctagg tgttcccgaa
    30421 ggtaaagaat tcatcaaacc aatcattgaa ttctactata aggatgacca gttggatgat
    30481 ccatttatca atgaagagca tatctatttc ttacaacttg caaaaccgga agaaatcagt
    30541 gagctgaaag cccaagcgtt ggctattaat gaaattttga ccgaattatt tgctaaggtc
    30601 ggtttggttt tagtagattt caagctagaa tttggccggt cgaatggaca aattttgctt
    30661 gcggatgaag taacaccgga tacttgccga ttatgggatg aacgtacgaa tgaacatatg
    30721 gacaaagacg tttaccgcag agatctaggt gatttgatcc cagtctatca agaagtatat
    30781 aaccgtctat cggaactaac tgaagaaaag gagcgagtat gatgtatttc gtaaaagtat
    30841 atgtaaccta taaagattca gtgttagatc ctcaagggga ggcagtaaaa aaagcagtcc
    30901 atcgtatggg atatgaaaca atcgaagata tccgaatagg aaaatacttt gagatacaac
    30961 ttgctgatgc agaagaacca atcgatacag tgattgaaac gatttgtgac aagctgcttg
    31021 caaatgtaaa tatggaaaca taccgatatg aaatcaaaaa aatggaggaa gtataacatg
    31081 aaatttgcgg tcatcgtttt tccaggttca aattgtgacg cagatatgtt atgggcagtt
    31141 cgcgagatca tgggcgctga tgccgaattt gtacggcatg atgagtcctc tttagaagga
    31201 tttgatgggg ttttactgcc gggaggattc tcctatggtg actatttgag atgcggtgcg
    31261 attgctcggt tttctacaat catggaagaa gtgattcgtt ttgctgaaga agggaaacca
    31321 gtattcggta catgcaatgg attccagata ttgacagaag caggactttt gccaggtgta
    31381 ctgagacgaa atgagtcttt gcattttatt tgcaaaactg taccattgaa agtcaacccc
    31441 aagacggcct ttacttcaga gtatgaagcc gacgaaataa tctatcttcc agttgctcat
    31501 ggggaaggaa attatttctg tgacgaagaa acacttcaag aactaaaaaa aaataatcag
    31561 attgccttta cttataatga caacatcaac ggcagcgtgg aaaacattgc agggattacg
    31621 aacaaagcag ggaatgtgtt aggaatgatg cctcatccag aacgtgcagt agaagacttg
    31681 ctaggatcag aagatgggaa acggttcttt gcttccattt taaaaaactt cggaaaggtg
    31741 accgtataga tgaaaacatt gaaagaaccg acagctgaag agatcaagaa aaaaagaatc
    31801 tatgccgaat ggggattgac tgatgaagaa tatcgaatga tcgaagaaga tatcttagga
    31861 agaatgccta attatacgga aactggactg ttttctgtta tgtggagcga acattgttcc
    31921 tataaaaatt ctaaaccggt tctacgcaaa tttcctacta gtggacctca tgtgttgcaa
    31981 ggccctggag aaggcgcagg catcgtcgac atcggtgatg gactagcagt tgtcttcaaa
    32041 gcagaaagtc acaatcatcc ttcagccgtg gagccttatg aaggagctgc gacaggagtt
    32101 ggcggaatca tcagagatat tttcagcatg ggggctcgtc caattgcgtt gctagattca
    32161 ttgaggttcg gcgaattaga taatccaaga acgaaatatt tactagaaga agtagttgca
    32221 ggtatcggcg gatacggcaa ttgtatcggc ataccgactg taggcggcga aattgcattt
    32281 gatccttgtt atgaaggaaa cccattagtc aatgcaatgt gcgtaggttt gattgaacac
    32341 aaacatatcc agaaagggca agccgctggc gtcggtaatt cgatcatgta tgtaggtgca
    32401 aaaacaggtc gcgacggtat ccacggagca acttttgcct cagaagaatt taatcaagaa
    32461 gaagaacagc aacgttctgc tgtacaagta ggcgatcctt ttatggaaaa gctgctgctt
    32521 gaagcgtgtt tggaattaat cttgcatcat tcggattctc taataggtat acaggatatg
    32581 ggagcagcag gacttgtttc atctagtgcg gaaatggctt caaaagctgg ctcaggactt
    32641 atattgacat tagatgaagt cccacaaaga gaaactggga tgacacctta tgaaatgatg
    32701 ttatcggaat cacaagaacg gatgttgatt tgtgttaaat caggagagga agaaaaaatc
    32761 caagaactat ttcagaagta tgatctagat gcagtcacta tcggtaaagt aacagacgac
    32821 gggcaatatc ggctatatca tcaaggagaa gaagtagcaa atctgccagt cgatgcatta
    32881 gctgaagatg caccagttta tcataaagaa atgaaagaac cagctcgaat cgtagaattt
    32941 cagcagttag ctccttatca gccagttatc gaagaacctg gacaagtatt gcttgatctg
    33001 ctgcagcaac cgacgattgc ttcaaaacgt tcaatctacg aaacttatga ttcacaagta
    33061 caaaccaata cagttgtccg tccaggaagc gatgctgctg tgatgagggt tagagggacg
    33121 aataaagcgt tagctatgac cacagactgc aacgcgcgct atctgtattt ggatccaaaa
    33181 atcggaggac aaatagcagt ggcagaagct gctagaaata ttattgctag cggtggtaag
    33241 cccttggcga tcaccgactg cctgaactat ggttctcctg ataaaccaga agttttttgg
    33301 gaattatcga cttcagctga cggaattgct gctgcgtgtg aaacgttagg aactcctgtt
    33361 atttcaggaa atgtctcttt atataatgaa acggatggtc aagcaatcta tccaacacca
    33421 atgattggta tggtcggatt gattgaggat cataaacata tcactactca ggaattcaaa
    33481 aaatcagggg atctaatcta tatattaggt aagacgtttg cagattttga tggcagtgaa
    33541 ttacaaaaga tgcagcttgg aagaatcgaa ggtgtgatcc gaaactttga tctatccatt
    33601 gaaaagagaa accaagagtt agtattgaca gccatccaaa acggattgat tgaaagtgct
    33661 catgactgtt cagaaggtgg cttggcaatt gcactggcag aatcagcttt caaacatcag
    33721 ctagggatat ctgtacagtt tgaattgtcc tctgctcaat tatttgcaga aactcaatca
    33781 agatttgtgt tgacagtcgc accggaaaat aagacacgat tcgaagaaat gatgggagat
    33841 gctgctgttt tagcaggtaa ggtgacggat gaagcgataa tcgaaatttc agcaactgac
    33901 ggtcagatca aaatagaaac tgcagtagca agaaagtgct gggaggatgc aatcgtatgt
    33961 cttatgaagt gaagagttta aatgaagaat gcgggatctt cggtgtttgg ggtcatccag
    34021 acgccgctag tgtggcatac ttcgggctcc atagtcttca gcatcgagga caagaaggtg
    34081 caggtatcgt gtccaatgat cgaggaaagc ttaaggggca tcgagatttg gggctgcttt
    34141 ctgaggtatt caaagatcaa agaaaattgc agcagttaac aggagaagct gcaatcggac
    34201 atgtacgtta tgcaacagca ggcaatggaa gtgtggacaa tattcagcct tttctattca
    34261 aatttcatga tcagtcgatc ggcttagcgc ataatggtaa cttgatcaat gctcgttccc
    34321 ttagaaaaga gttagaggaa aatggggcga tcttccattc taactccgat acggagatat
    34381 tgatgcattt gatccgacga agtgaagaaa cagatttttt aaataaatta aaaagtgctt
    34441 tgaatgaagt caaaggtggc tttgcctatt tattaatgac agaaacggca atgattgccg
    34501 ctttggaccc aaatggtttt cgtccattag caatcggaca aatgaaaaac ggagcatacg
    34561 tgattgcctc ggaaacttgt gcattagaag ttattggggc acgttttata agagatgtcc
    34621 agcctggtga gatagtgatc atcaatgacg aaggaatcca gattgattgc tttacgaagg
    34681 acacgcagct ttctatttgt tcgatggaat atatctattt tgccagacca gattcaaaca
    34741 tcgcagggat caatgtgcat actgcacgga aaaatatggg acggaattta gcgaaagaat
    34801 cgccagtaaa agcagatatg gtcgttggcg tacctaattc ttctttgtca gccgcaagcg
    34861 gttatgcgga agctagtgga atcccttatg aaatcggatt agtaaaaaat caatacattg
    34921 cccgaacatt tatccagcca acgcctgagc taagagagca aggtgttcga atgaaattat
    34981 ctgctgtacg aggagtagtg gaaggtaaaa aggtgattat ggtcgatgat tcaattgttc
    35041 ggggtacgac aagcagacgg atcgtccatc ttttgaaaga agcaggggca aaagaagtcc
    35101 atgtgaggat tgcttctcct cctttgaaat atccatgttt ttacgggatt gatattcaaa
    35161 cgcgtaaaga actcattgcg gcaaatcaca cgattgatga aatcaaagaa tgtatcggtg
    35221 cagattcttt aggtttttta agtgaagagg gtctgatcga atcgatcggt ctggaaaaag
    35281 atgctccgta ttcaggattg tgtatggctt atttcaacgg agattatccg acgccgttat
    35341 atgactatga agaaaaatat caggaatcac taaaagaaaa agtatcgttc ttttaaaagc
    35401 agaccaagaa gggagtatat gaattcaatg gcaaatgcat acgcaaaagc tggcgtagat
    35461 gtcgaagctg gatatgaagt agtagaacgg atcaagaagc atgtcaaaaa aacggaacgt
    35521 ttaggtatga tgggcgctat aggaggattc ggcggttgtt ttgatttatc ggtctttcaa
    35581 ttaaaagagc cagtccttgt ctcggggacc gatggcgtgg ggacgaaatt agcagtggca
    35641 atcgaagcag acaaacacga tacaatcggg atcgactgcg tagcgatgtg tgtcaatgat
    35701 atcttagcac aaggcgcaga gccgttatat ttcttagact acttagcaac ggggaaaaat
    35761 gatccgttac gtttggaaaa tgtagtaaaa ggtgtagcag atggatgtgt tcaaggaaat
    35821 atggcgctga ttggcggaga aacagctgaa atgcctggta tgtactcagg taatgattat
    35881 gatctagccg gatttgctgt agggatagct gaaaaatctc aattattgac aggagaaacg
    35941 atcaacccag gagatgtgtt gcttggaata gcatctagcg ggattcattc aaatgggtac
    36001 tctcttgttc gcaaaatttt ctttgagcag cagcagtttt ctttatcatc aaaactgcca
    36061 gaattgaaaa aaacacttgg cgaagaattg cttgagccaa cgaaaatcta tgtttcttcc
    36121 ttgcttccgc tgatcaaaga aaagaaaata aaaggagctg cacatatcac tggtggaggg
    36181 tttgtagaaa atatcccgcg aatgttacca gaatctatgg ctgcacaaat caaacttggc
    36241 tcatggccga ttcttccaat attcggctgt ttacaaaaat atggtgaaat tcctgagatg
    36301 gagatgtatg aaatcttcaa tatgggcatc ggtatggtcc tggcagtcga agaatcgaaa
    36361 gtaaaagaaa tagtacacga aattgagcaa gctggcgaaa aagtttatga aatcgggaaa
    36421 gtgatcgcca aagaaaaaca tcgtgtggtg tttataggag aagaacgatg agaatcgctg
    36481 tttttgcttc tggaaacgga agcaactttc aagcgctagc cgattatcta tcgaaaaaag
    36541 gattggaatc ttcgattgac tggttgtttt gtgatcagcc tgaagcatat gttttgaaac
    36601 gtgcaactgc gttgagtgta ccggcagatt gtttttcacc taaagaattt gattcaaaaa
    36661 aagagtatga agaagcaatt ttgcataaat tgaaagaaaa aaagatcgat ttgatcgtat
    36721 tagctggata tatgcgaatc attggaccgg tattgctgaa aaattatgat aaaagaatca
    36781 tcaatatcca tccgtctctt ttacctgcct tccctggttt gcatggcata cgtgatgcct
    36841 ttgaagctgg agtaaaagaa acgggtgtca caattcatta tatcgaccaa ggtgtagata
    36901 ctggaccaat catccggcag gaaaaagtga gaattgaaca ggaggacacg ttcgattctt
    36961 tagaagaaaa aatccataga gtagagcatc gaatctatcc agaagtgatt agtgaaatta
    37021 ttgaaaatgg aggctatctg aaatgacaag agcattgatc agtgtatcag ataaaactgg
    37081 gattgtagca ttcgcccaag gtttgattga acaaggagta gaaatcattt caacaggagg
    37141 aacaaagcat gcgttggata aagctgggat caaaacgctt tcgatcgaag aagtgacagg
    37201 gtttcctgaa atgatggatg gccgtgtcaa aacacttcat cctaaaatcc atggtggtct
    37261 tttaggaaga cgcgatctcc ctagccatat ggaggcaatg gaaacggaaa atatccaacc
    37321 aatcgacttt gtttgtgtca atttatatcc tttcaaagaa acgattatga aaccagatgt
    37381 cgaaatagca gaagctattg aaaacattga tatcggtggt cctagtatgc tccgtagtgc
    37441 agcaaaaaac catgaatttg tcacctctgt tgttgatcca tctgactatg aagaagtact
    37501 ttcagaatta cgtcagtatg ggcatacttc actttctttc agacaaaaac tagcggcaaa
    37561 agtctttcgt catactgcgg catatgatgc actgatcgct caatacttaa cggattgggt
    37621 ggaagaaaaa gaaccggaaa aattgacact tacgtatgaa cgaaaacaaa cgcttcgtta
    37681 cggtgaaaat agccatcaag ccgctgcctt ttatcagtcc gctcttctgg aaaaatattc
    37741 gatcgcagca gcaaaacaac tccatgggaa agaactttct tataataata tccgagatgc
    37801 agatgctgcg ctacggatca tgaaggaata tgacgagcca acggtagtcg ccttgaaaca
    37861 catgaatcct tgtggtattg ggacagcatc tacgatttta gaagcgtggg aagcagcgta
    37921 tgaagcagat cctgtttcaa tctttggtgg gatcatcgtt ttgaatcgag aagtagatct
    37981 gccaacagca gaacagatgc ataagctgtt tctagaaatc atcattgccc cttcttatac
    38041 acaagaagct ttggaattac tgcaaaaaaa gaaaaacatc cgattactga cagtagactt
    38101 tgcgcaggaa gaaaatcaaa atcgagaaga aacggtctca gtcttaggtg gacttcttgt
    38161 acaagatcaa gatctggcaa tagaaaatga agaagagtgg aaagtcgtga caaaacgtaa
    38221 gccgactcca gcagaagcaa aagcattagc gtttgcttgg agagctgtca aacatgtgaa
    38281 atctaatgcg atcgtactgg caaacgaacg tcagacagta ggaatcggag ccgggcagat
    38341 gaatcgagtc ggttcagtaa aaattgcgat tgaacaagcg caagagaaaa tggaaggtgc
    38401 agttttagct agcgatgctt attttccaat ggatgattcg gttgaatatg cagccaaaca
    38461 tggtataaaa gcaatcattc agccaggagg aagcatcaaa gaccaagatt ctattgatat
    38521 ggcgaataaa tacgatctag caatggtctt tactgatgtt cggcatttta gacattagag
    38581 aggagtcgga taaatgaaca ttttggtgat cggtgcaggt ggtcgtgaac atgcaataag
    38641 taaaaaactt ttagccagtc cgaaggtgga tacggtgtat tgtgcgccag gaaatccggg
    38701 gatgacaaaa gatggtatca attgtgtcgc tatcagtgag cgagatcatt cagcgttaat
    38761 ccattttgtt aagaaagagg ctattgattg gagtatcgtt ggtcctgaag ttcctttgtt
    38821 aaatgggatc gtcgatgatt ttatggcgga aggattgaaa atcttcggtc cgacaaaagc
    38881 agcagctctg atcgaaggtt cgaaaagttt tgctaaacaa ttaatggata cttatcaaat
    38941 cccgacagct aaatccagaa gttttgaatg ttacgaaaaa gcagcagcgt atatcaaaga
    39001 aaatggtgcg ccaatcgtag tgaaagcgga cggcttagca gctgggaaag gtgtagtcgt
    39061 ggctaccaca gtgaaggaag cgctggatgc ggcagaacaa atgttgaacc agcatcgatt
    39121 tggggatagt agcaaaaaaa tcgtcgtaga agagtttttg gcaggagaag aattctccct
    39181 tttggcgttt gtccgtaata ccaaggtgta tccgatggtc atttcgcaag atcataagcg
    39241 ggcatttgat aacgataaag ggccaaatac aggcggaatg ggtgcttatt caccagtacc
    39301 gcaaatttca aaaaagatga ttgaaaaagc tgtggaagaa gttttaaaac cagcggcttt
    39361 gggaatgaaa gaactaggac gctctttcac gggtgtttta tatgcgggtt tgattgctac
    39421 gaaggaagga ccaaaagtga tcgaattcaa tgcgcgtttc ggtgatcctg aaacgcaagt
    39481 cgttctttct cggttgaaga gtgactttgc gcaagtaatc gatgatttat tagagaatcg
    39541 tacagtagag ttaaagtggc agcaagaagg atacaatgta ggcgttgtgg ttgcagctgc
    39601 cggttacccc gaagaatatg agacaggaat ggttctacca gattttctgg aagaagagct
    39661 ttctgtttac tatgctggag tttcctcaaa taacggcaaa ctagttggag caggaggacg
    39721 tctttattta gtagaagcat acggtgagac gatcaaagaa gcacagaaaa aaatttacac
    39781 cgcgttaatg gataaaacga cagatggaac attttatcgt acagatattg gagaaaaagc
    39841 tgccaatagc gaactgatcg tgaagtaaat cagctgcttc catttataaa aaatacaaca
    39901 aacataatcg gaagcgccga ttttagtgaa gccttttcat aaagagtgtg gtacactttt
    39961 gttgtgtata taaataagga ggagttcaga tgaaaaaaat tcaattaggt acttctgatc
    40021 agaaagcagc tgcagtgatc ttaggctgta tgcgtatcaa caatgcggaa gatccagtaa
    40081 aagtgattga gacagcttat gaaaacggca ttgatttttt tgaccatgca gatatttatg
    40141 gaaacggtga atgtgaagaa atctttgcag atgctctagc aaaaacatca atcaaacgag
    40201 aagatctttt tattcaaaca aaatgtggaa tcgttccagg tgtgatgttt gatttttcta
    40261 aaaaacatat cattgaatct gtagaaaaaa gtttgacccg tctgaaaatg gattatgtgg
    40321 atgctttact tcttcatcgt ccagatacat tggtagaacc agaggaagta gctgaagcgt
    40381 ttgatgaatt ggaacggtca ggaaaagttc gttattttgg cgtcagcaat caaaaaccaa
    40441 tgcaaatcga attgctgaaa aaatatgttc gtcaaccatt agtagctaat cagctgcaat
    40501 ttggcttgaa acatactgga atgatcgatc aagggattca tgtcaatatg acagatgaag
    40561 caagtatcga tcatgacggt agtgtacttg attattcccg attgcatgat atgacgatcc
    40621 aagcatggtc accataccag tatggatttt ttgaaggtgt atttatcggg aacgaaaaat
    40681 tccctgaatt aaaccaaaca ttagaagaac tagcagctaa atatcaaacg acaccaactg
    40741 gcttggcttc tgcttggatt ttacgccatc cagccaatat gcaagtgatc gccggtacga
    40801 tgaacgtagg gcgaatcaag gaaatcgctc aagcttcaga aattaatatg tctcgggaag
    40861 attggtacga attgtaccgt gctgctggaa acattttacc ttaaagattg tcataagcag
    40921 gtcaaaaggg ctataaatta aaaagacaga ataacttgaa agctgttttc agcttcgaga
    40981 tattctgtct ttttttattg caaatggaaa gagtatcatt gtacggattt cgtttattag
    41041 ttatttttgt ttttaaggaa aagctgtgtc cgatattctt ttggagaaat cccttgtttt
    41101 ttcttaaata aataactgaa atagttaggg tcattataac caatttcaaa agcaatatct
    41161 gaaatacgat agtctgattg ctgcaataat ttttttgcta attcgattct ttgttctgtc
    41221 agataatcga tgaatgtttg gttaaacgat tgagaaaaaa tcgtactgaa atgagaggga
    41281 cttaatgcaa cttgttgagc gacccaagtt aaggacatat ctggatcggt aaaattttct
    41341 ttgataaaat ccaatgcatg gttcaaaatt ggctgatatt tgttccttga aggctgtgag
    41401 ggcagagcag ttaagaaaga aatgaacata taaactgtcg aacggtaaag cgagtcgtcg
    41461 gcgctgattg ctaagcgttg actggtggat gtgcaatgaa ccatttcttg cgggattttc
    41521 ttttctttca ggcagacatg ctggagtagc ttagtcaaga caaataaacg atataatcgt
    41581 gttcgagttg gtgtgccttg tggtttcgtc aagtgttcga tgtacttaga tacttcgttt
    41641 ttcgataatg aagacaaatc aagtgagagg tcaaagtaaa agacagaatc aactggaagt
    41701 ttcgcttctt gaatcaaatc ctcatagctg ataatttgtt ctttaaggga actggcggtt
    41761 tcaagtatca tctgcgtgat ctcaaaagat gcaggtaatt cgctgattct ttctgcgaca
    41821 ggaccaatgg cgacagaaaa attatttttt tgtccctttt ctaattcatg gaccaacgtt
    41881 tgggctacct gataactggt ctcgatcaca ttttcttcaa gagaatcaac tattaaaaat
    41941 ttgatgaatc gagaggaaac agcagagaag atcacttgtt gattctccct aaataaaaac
    42001 ttcaaataat cactgagatg agcgtaatct tcaaagtctg tactgacttg gtttgctgct
    42061 aataatacgg tcattttttt ccctgctaaa gagcgattga aagattcagc aagtttcaat
    42121 gtttcactga tcgtaagtga gcctttgaac aaaccgttca agaaatgatt ttttttcaat
    42181 tcgaaaacaa gatcagaaga agtgtgcgtt tctttttgct gcaaatctaa agcagttact
    42241 gctttttcta ttgcttgttt caattcgcta cttttaatcg gtttaagcag ataatcatct
    42301 gcctgtacct ggattgctcc tttcatataa tcgaaatcgt cgaatccact aataaatatg
    42361 agacgagtcc aaggaagtag tttccttgct tcttttgaaa aagtcaaccc gtccatgaaa
    42421 ggcatttgga tatcagtcag aatgatgtcg ggctgtaagt cgataattga agacagtgcc
    42481 atctcaccat ctccagcttc gccgacataa gtgattggat aataagaacc aaggacagtt
    42541 atttgttccc gcaagttatt gcgaatgagg tattcgtctt cgatgataaa aactttatac
    42601 atgtttgttt cctccaattg ttttgggaga tagagggatc atcagtgtga cagttgttcc
    42661 ttctcggtat ttgctgtcaa tcgtgatagg tttttgatca tttccgtaat aaagaaggag
    42721 acgcttgttt acgttgtaaa gtccgtagcc gatactgaaa tctgaatcga tccctttggc
    42781 taactcttta cgtatttctg ccagtttttc tgcagggatg ccaatcccgt tgtctataac
    42841 tttgaattgg atcatctcat caattttgca tgcggtaata cgaataaatc ctttgtgacg
    42901 gacgtacttc gttccatggt atacagcatt ttcaatcaat ggctgcaaga tcatttttaa
    42961 aatagaaaca ggaaaaagtg tttctggtat atcgatctta tagtcaagca tatggccgta
    43021 tcttattttt aagatgacta gataatcatt gatatggcta atttcttttt caagaggaat
    43081 ccagtcttgt cctttgctta aagacgtacg aaaaaagccg gaaagtgcat gggtcatagt
    43141 cagagcttct tcaaagcagt cttgttcgat caaagaaata atggcatcca aactgttata
    43201 aataaaatgg ggagtaatct gagcttgcaa tacacgaact tcactttgtg ctaaatggta
    43261 ttgtttcaaa gcgttctccc gtaaagactg gtttagatct tccgccattt tattcatgct
    43321 agcggtaagt gtttgtaatt ccagtgtttc cggcagggtt aaacgaaagc caaggttccc
    43381 tgaagaaagt tgttctgaca tctcgatcag ctgattgatc ggttcttgga cctttctctc
    43441 taaaaagcga cgattctttt tcgcatagaa aaaaatggta ctaagaactc caagttccac
    43501 tattgttaat agaatgagcg agcggacaag tgtcgtattt tttttgcttg ctaagtcgat
    43561 ctccacacga acaaaatctt gtaagatatc tgacagcagt tgagtgacag aatctacctg
    43621 attgatgatc tcttcattgg tatccataaa atcttttgcc aagatattat tgatcagctc
    43681 gttttgataa tcttccagtg tatcgatcgt acgtagcgat acatctaacg tactaatttc
    43741 tttaagtgta tttgtattat ttttgatctc tcgaatatca ttcctaagtt cttctaggat
    43801 attttcctca ttgaactcat ctacggttac ttggccagaa atcaagtccc acatactttc
    43861 caaaactttt tgatcaacct tcaaagcaat tgcatctgct ttttggatat ttgcgatcgt
    43921 ctgttgataa atcaaaagct gttgcgaata taacagtatg ctcagtagca aagggataac
    43981 agcaaataga agcatggctt tgtttgcttg ggcaaaaatc tgttttatgc ttaaattttt
    44041 tgtcatgata cttctccagt caataatttc tggttgggat gttagctata ttctgctggg
    44101 aaaagacagt ctcggaaata aaaatatctt taggtaatgt ttttcctgtt tgaatatttc
    44161 caaaataacg agtaacggta ttggctacat accagccgac atcaggttta cactcgacta
    44221 cacaattgac gattcctgag cgtagcttct cgatcatatc ttcttgtcca tcaatagtta
    44281 caatgatcaa atcttctcca ggtttgattg atgtttcttt tatcgcagct aaagcaccgt
    44341 gagtcatttc atcactatgg ctgtataata catcgatttc ggataaatca tttgtttcta
    44401 aatatcgttt catctggtct tttcctttca tccgaataaa atcaccgaca attcggtgaa
    44461 caatagtcat cggaggttta tggtcgatgg ttccatcttt ttttagggtt ggtttacgat
    44521 tgattgattc caaaaatcct tcggagcgta gcttggtcgg tgtggagttc tctgaaccag
    44581 caagttctag gattctgatt tcctgtttct ttgaatttgt gaagtgattg ctgacaaata
    44641 aacccgcccg atttccttct gctttgaaac tggggccaat gtgggtgacg aaaagctcgg
    44701 gatcattcgt ttgaatatgt ctgtcgacaa gtatgacagg gatccctgct cgctttgctt
    44761 cttttaatac agagtcccac ccggattctt gcaaaggagt aaaaatgatc atatccactt
    44821 tataggcaat gaacgtccgt atatcttgga tttgccgttc ctgattcatg tagccatttc
    44881 ggtagagaac ttggtaatct tctttttcaa gcgcagtgat aattgattca gtatgctttt
    44941 tccgccagct actctccgtt cctgattgcg aaaaaccaac gactagtggg tcaggtgctt
    45001 cctttgtatt aggtgtacac cccatcattt ggtaagcgat tacaacaagg gctaaaaaaa
    45061 ataaaacacg tttcacttta aatcactcca tctggatttg ttagtactat cttatcagat
    45121 aaaagggaaa atcaaagtca aaaggaatca atcgaaaaaa atccaagaaa cttaaaaaaa
    45181 tccaagacaa aaaaagtaca cctaaaaaat ttaaagtaaa tcccataaaa aaatcaatgt
    45241 taacgctttc tttattagct atactcggtg tatcaattga aatgggggat aaaaaatgaa
    45301 aaacaaattt agaaagattg ttgggactat cgctattttg accgcaagca ttctattaag
    45361 tgcgtgtggc aattcaggat ctgcagatga tagtacgggg tatgtaggaa tcgctatgcc
    45421 gaccaaatca gcagaaagat ggatcgctga tgggaacaac atggtctcgg aattagaaaa
    45481 actgggttat aaaacagatc ttcaatatgg agaagataaa gtagaaaatc aagtagcaca
    45541 aatagaaaat atgattacaa aaggggtaga tacacttgtg attgcttcta tcgatggttc
    45601 ggctttaact gatgtgttag ctaaagcaaa agaagcagat atcaaggtga tcgcctatga
    45661 tcgtttactg atgaattcag aaaatgtcga ttattacgcg acttttgata attttggagt
    45721 aggtgtatcg caagcagctt atattgaaga gcacttagga ttgaaagaag gaaaaggacc
    45781 attcacgatt gaattatttg gtggctcgcc tgacgacaac aacgcactaa tcaattacaa
    45841 cggagtgatg tctgttttac aaccatatat ggacaacggt cagttggtcg tgccatctgg
    45901 acagacgagt ttcagtcaaa tcgctacgct tcgctgggat ggttctacgg cgcaagcaag
    45961 gatggataat ctgttaagcg caaattatac tgaccaaaca ttggatgctg ttttgtcgcc
    46021 atatgatcca ataagtttag gaatcatttc gtcattaaaa ggcgtggggt atgggagtga
    46081 aagtaaacct ttacctgtga ttactggaca agatgcgaca gttgctggcg ttaaatcgat
    46141 cattgccggg gaacaaacac aaacgatctt taaagacaca cgtatcttag caaaaaatac
    46201 gattgaaatg atcaaagcaa tcagtgatgg agaagaggtt ccggtcaatg atactgaaac
    46261 ttatgataat ggggtgaaaa ctgtccctac gtatttagca aatacagtat ctgtcgataa
    46321 agataattat caagcagagc tgatcgacac cgattattat aaagaatccg atctgaaaaa
    46381 ttaagaggaa ccaaacaagg aactaaagag atctaagtca gagagaggat aagaattatg
    46441 gcggattgta ttttaaaaat ggcagatatc accaaaaaat tttctggaat ccgagcgtta
    46501 gattgcgtga atctgatgat tgaacgtggg gaaatccatg cattgtgcgg agaaaacgga
    46561 gcgggaaaat caacattgat gaatgtcttg agcggcttgt atccatatgg ctcctatgac
    46621 ggggaaatct tttatgatgg aaagctgtgt caatttaaag atttgaaaga tagtgaagaa
    46681 aaaggcatcg tcatcattca tcaagaatta gcactaagtc cttatctttc aataaaagag
    46741 aatatttttc tcggaaatga acaagcagaa agaggcgtga ttgattggga actgacagaa
    46801 aagaaaacat atgaactgct gaataaagtc ggtttgtcta tcgacccgaa tcttctagta
    46861 tcccagattg gcgtagggca acagcaacta gttgaaatag ctaaagcatt atcaaaatcc
    46921 gtccgtctgt tgattttgga tgagcctacc gctgcattga atgaagaaga aagcgcgaat
    46981 ttgctgagat tgatccgaga gttgaaagag caaggtgtaa cctccatcat tatttcacat
    47041 aagctgaatg aaatcgtgga tatcgcagat cgaatcacca ttttacggga tggacagacg
    47101 attgagacac tagaaaatga aatgatcagc gaagagcata ttattcgcgg gatggtgggc
    47161 agagatttag ttgatcgtta tcctgaaaga aaaccggata ttggagaagt ttattttaaa
    47221 gtagaagact ggacggtcta tcaccctctt gatcacgagc gaatcataaa cgaccacatc
    47281 aacttgcatt tgaaaaaagg agagattgta ggaatagctg gactgatggg agcgggcaga
    47341 actgaattcg ctatgagtct atttggacat tcttatggaa gcaagatatc cggacgtata
    47401 ttaaaggaag gggcagagat atcagttaag accgttcctt cagccattgc aaacggactc
    47461 gcttatgtct cggaggaccg aaaatcttta ggactgaatc tgctgatgga tattcgggaa
    47521 aacgtgacgc tgagcagttt ggataaaata agtcaaaaag gagtattaga taaagaaaaa
    47581 gaagtaactg aggcagaaaa tttccggaaa aagatgcgta taaaaacaaa ctccatccat
    47641 caaattgtca gtagtcttag tggtgggaat caacaaaaag tcgttttatc caagtggctg
    47701 atgacacaac ccgatattct ttttttggat gagccaaccc gtgggataga tgttggggca
    47761 aaatatgaga tctacacgat tatcgaagaa atggcagcaa atggcaaagc tgtctgtctt
    47821 atctcttctg aacttcctga aatattaggg atgtgtgatc gaatctatac gatgaacgaa
    47881 ggacgtatta caggagaagt ctcgcgagaa gatgcagatc aggaagtctt gatgaaatta
    47941 atgacgaaag aagaggtggg ttaagttgga acaggcaaaa aatcttacgg ataaaaaaga
    48001 gaaaaaacta aatttgaaaa gtagagcagc tgatcttttt ggtcgctaca gcatggtgat
    48061 cattttagcg ggactactta ttgcgtttca attgatgacg gatggtattt tctggcgtcc
    48121 attgaatata actaaccttg ttctgcaaaa tagccatata ttagtactag ctgcagggat
    48181 gttattagtt gtgctcttag gtcatgtgga tttgtcggta gggtcagtga tggcgtttgt
    48241 tggtgcaatt gcgggcatga tgatggtcaa tcgccaaatg aacccgtgga tgacagttat
    48301 cgcatgtctt gcagtaggcg ggctgatcgg cgcatggcaa ggtttttggg tagcttatgc
    48361 agggataccg gcgtttatcg tgactttagc aggattgctg atgtttcgtg gactgacaca
    48421 agttgttcta ggaggccaat ctctagcacc ttttccagct gttttccaaa aaatgtctac
    48481 gggttattta ccagacttaa gcgaaggaag cagccttcac ttcttgacat tgattttagg
    48541 aatgatcatc acgatcgtgt tagttgttgg tcaatggcga acaagaaatc gtcggaaaaa
    48601 tcatttattt gaagtggaat ctccaacagc tttcttggta aaggcggtat ttacagcagt
    48661 cgttgtacta ggaataactt atatctttgc ctcttatcaa gggtatccga ttattttgat
    48721 cattcttggt ttgatcgttg cagcatatgg gtttttgaca aataaaacag tagcaggtcg
    48781 acagatttat gcgacgggcg gaaatcgaaa agcggctgaa ctatcaggga tcaaaacaaa
    48841 gcgaatcacg ttctgggtat tcgtcaacat gggagtcatg gcagctttag ctggattagt
    48901 tttagctgct cgattgaatg cggcaacacc tcaagcaggt acatcgatgg aactggatgc
    48961 aatggcggca gtatattttg gtggtgcttc gacttccggc ggcattggaa caatcatggg
    49021 tgcgattgtc ggtggcttgg tcatgggtgt attgaacaat ggcatgtcga ttttaggcgt
    49081 cggggtagac tggcagcagg caatcaaagg attgatcttg cttttagccg ttgtgctaga
    49141 cttgtataat aagaaaaaac gaacaacgta ggagaacgcc ggttattcag aaaaaagact
    49201 gtgacaagac atttgtcaca gtcttttttt cgctaaaagc gaacattcat tgtttcgttt
    49261 gtatagacta aaaataatat atgttttttt accattcgta taaattctct ttttgctctt
    49321 gatttttttg cttaattgag ttaaattaca tagggcgcta aaaaaagcct tgattaaatt
    49381 aataatgttc gtgttttgct ggatgtgaac atcaggagga taatgtaatg aaagtatttg
    49441 attacgaaga tattcaatta attcctaata aatgtatcgt aaatagccgt tctgaatgtg
    49501 atacaacggt cacattaggt aaacatacat tcaaaatgcc agtagtgcct gccaacatgc
    49561 agacgattat tgatgaaaca atcgcagaat tcttagcgga aaatggttat ttctatatca
    49621 tgcatcggtt tgatgaagca gctcgaattc cgtttatcaa aaaaatgaaa aagcgtggat
    49681 tgattacctc gatcagtgtt ggcgtcaaga aagaggaata ttcctttata gagaagttgg
    49741 cagaagaatc gctaaatcct gattatatca ctatcgacat cgcgcatgga catgctaact
    49801 cagttattga tatgattcag catattaaaa aatatttgcc tgaaactttt gtgattgccg
    49861 gtaatgttgg tacaccggaa gccgtccgtg aattggaaaa tgcgggcgca gatgcaacaa
    49921 aagtcgggat tggtccagga aaagtatgta ttacaaaaat caagacaggc tttgggactg
    49981 gaggctggca actggcagct cttagatggt gtgcgaaagc agctcgcaag ccgatcatcg
    50041 ctgacggtgg gatcagaaca catggcgata ttgcaaaatc tgtccgtttt ggtgcgacaa
    50101 tggtcatgat tggttcattg tttgccggtc acgaggaatc tcctggcgaa acaaaagtcg
    50161 aaaatggaat cgtgtataaa gaatactttg gttctgcttc agaattccaa aaaggcgaaa
    50221 aacgaaatgt tgaaggaaag aaaatctgga tccaacacaa aggatctttg aaagatacac
    50281 tagtggaaat gcaacaagac cttcaatcat ctatttctta tgctggagga agagatctgg
    50341 aagccattcg aaaagttgac tatgtcatcg tgaaaaattc gatttttaat ggagacacaa
    50401 tctaaaaaat aaataaaata ttaggatgtt gtgaaaaagt tgtcctgcat taagtaataa
    50461 gaacagccaa acaaaaatag ttctttatat gttttgtttg gctggaatta ttatcgcaga
    50521 tacaagcttt tgaacatcat tattcagaat aagggactgt gataagattt ttgtcacagt
    50581 cccttatgtt ttgcaaaaaa agtttttctt ttttctcgta aattcggtaa aatcaaaaga
    50641 aaaaatagac aatagaaaag agaagcttat gtgggaaaaa gtagagaaaa aacaaggacc
    50701 gatttatcgt caaattatgg aacagattat gagagaaatc gaaaacggaa ggatcaatcc
    50761 tggaagtcaa cttgaatcag aacgaaaact agggcaactt tttggtgtga atcgttccac
    50821 cgttgtccat gcattggaag agcttaggga gcttggggta ctaacgagca aacgtggcag
    50881 cggatggttt gttaatcaga ctgaatgggg gaaattcgct gttccaagaa tcgattggcg
    50941 gcaaatgatc tctcctcgtt atgagcagac ggactggtat gagcaaaaaa taagagaagc
    51001 aaagcagatc caaaaaacat cgtttcttga tttatacgct agcgagatgc cagatgagct
    51061 tttgccgaat tttgagtttc cttcttattc tcttgaagaa atcttagcgg aagaaaaaga
    51121 aatggatctt ttaggctata ctccgttacg taacaaaatc aaacggtatt tggaaagtaa
    51181 atttgaattg gcattttccc ccaaccagct tcttgtgact ggtggcggtc agcaagcaat
    51241 ctttttgatt ttacaaacga ttctttcagc tggagagggg atagctgtag agtcaccttc
    51301 ttttttttac cgattacctc ttttcaaagc ttcaggaacg cgtttatttg ggataccgat
    51361 ggatgaagaa gggatcgact tagaaaaact gaaagaatcg atccataaaa ataaattgaa
    51421 agcagtgctt atcaacccga attttcaaaa ccctacagga aaagtgatga gtcaaaaacg
    51481 acgagaaaag ctagtagaat tgtgtcgaca gtatcggctt ccaattattg aagacgatgt
    51541 atttacggat ctttcatttc taaaccaaga agaagtcctt cctatacgaa cgattgatcc
    51601 ggataatgtg ttatacgtcg gttcgttatc tagggtttta ggaaaaacaa cgaaaatcgg
    51661 ttgggtgata ggacctgaga cttttatctc gcaactagct caagcgcaag aaatgatgga
    51721 gttttcgatg aatatcctta cacaaatcac agtggctaat gtatttaatg aagcaattga
    51781 tttgaaaatg caagatgtaa gaaaaaagtt gaaagaaaaa agccgactga taagcgcttg
    51841 gggagattca caatcgtttt tcaagcttca ttcaatttta ggtggttact atgcctggat
    51901 tacttgggat gggaaaaaac tagaccccga actagcagaa aaagtagtag atatgggtct
    51961 tggaattgca ccaagtctac tttttgggca agatataaat ggaatcagaa tcaattacag
    52021 taggctggat gaaaaccaaa taccgttgtt ttattcaaag atgaaaattc tagaaaagtg
    52081 gttaacagaa taatttcaca aagtaaatag attaaagaat ataacagctt ggatttaaat
    52141 ttcaagctgt ttcttttttt tcggaaaaag aatacgatat aaagaaacta agaaaaaacc
    52201 attatttgta cgtccgttca cgatgtgtaa ataaaataaa tgtttgcaac aaaactgtat
    52261 aacaccaacg tttttaaata gaaacagatg gttgtaagtt tgattagaaa atagaaaaaa
    52321 tgcagatgaa atataaaacg ctttccttat tgtgatttta ttcactaatg ctgttaatat
    52381 gcggattggc ataatttcct ttcatcgtga aaatttgtgc ttttagcttg tatttctcat
    52441 aaatccatga taatttatta atgaagacgg ttaaatagtg atacagtttt attcactgtt
    52501 tcaatcgcag aatgaagaga ttttcttata gatgagagag gaatgtgtga caaatggcaa
    52561 acaaaaaagc accgatcgac ttccaagcac tgctagatga agtcaatgca gatttcccag
    52621 tatatcaagc acttgatcaa gatggaaacg tagttaacaa agatttagta cctgatttat
    52681 ccgatgatga actcgttgaa ttaatgactc atatggtatg gtctcgtgta ttagaccaac
    52741 gttcaacagc gctgaaccgc caaggacgcc taggcttttt tgcaccaact gctggacaag
    52801 aggcaagtca gctggctagt gcttacgcat ttgataaaga agatgttctt cttccaggtt
    52861 atcgggatgt acctcaacta gtaaaacatg gtttaccttt atctcaagct tttttatggt
    52921 ctcgaggaca tgctgcaggt aattcttatc cggaagaatt gaaagctcta ccaccacaaa
    52981 ttatcatcgg tgcacaatat gtacaagcag ccggtgttgc attaggtttg aagaaacgaa
    53041 acaagaaaaa tgtcgtcttc acttatactg gtgatggcgg ttcatcacaa ggtgattttt
    53101 acgaagcaat caatttcgct ggcgcttacc atgcaaatgc cgtattttat atccaaaaca
    53161 acgggtatgc gatctctaca cctcgtgaaa aacaaactgc agcaaaaaca cttgcacaaa
    53221 aagctgtcgc agctgggatt cctggtattc aagtcgatgg aatggatccg cttgcagtat
    53281 atactgtatc taaaatggca cgggaatggg cgatcagtgg aaatggacca gtcctaatcg
    53341 aaacactgac ttatcgttat ggaccacata cactatctgg tgacgatcca acccgctacc
    53401 gtacaaaaga cacagatgac gattggcaga aaaaagatcc attgatccgc ttccgtaaat
    53461 atttgacgga aaaaggactt tggtctgaag aaaaagaaga acaagtcatc gaagcaacta
    53521 aagaagaaat caaagcagct attgctgaag ccgacaaagt accaaaacaa aaggtttcag
    53581 atttcttgaa aaacatgttc gaagtatctc cacaatcaat caaagaacaa attgcaattt
    53641 atgaagcgaa ggagtcgaaa taatcatggc acaaaaaaca atgatccaag caatcacaga
    53701 tgccttagca cttgaactag aaaatgacga gaatgtcgtt gtttttggtg aagatgttgg
    53761 gaaaaacgga ggagttttcc gggcaactga aggattgcag gaaaagttcg gagaagaccg
    53821 agtattcgat actcctttag ctgaatctgg aatcgcagga ttgagtttcg gtcttgcgtt
    53881 agaaggattt cgtccagttc ctgaaatcca attctttggt tttattttcg aagcaatgga
    53941 cgaagtagta gctcaaatgg ctcgtacaag atatcgaatg agcggcacta gaaatctacc
    54001 catcacaatt cgttcgccat ttggtggcgg tgttcataca ccagaattgc attcggataa
    54061 tttagaaggt ttgatcgctc agtctcctgg tattcgagta gtgattcctt ccaaccctta
    54121 tgatgcaaaa ggactattga tttcttctat tagaagtaac gatccggttg ttttcctaga
    54181 acacatgaaa ctatatcgct cattccgaga agaagtgccg gatgaagctt acgaagttcc
    54241 tttggataaa gcagcagtta ctcgtgaggg aacagatgta tctattatca catacggagc
    54301 aatggtgcgt gaagcaatca aagcagcaga caatcttgca aaagaaaata tatctgttga
    54361 aatcattgat cttcgtacag ttgcgccatt agatgtagaa acgatcattc aatccgtgga
    54421 aaaaactggt cgcgtagttg ttgtccaaga agcacaacga caagcgggcg ttgccgcgca
    54481 agtagtttct gagatctcag aacgcgctat cctttcatta gaagccccaa tcggacgagt
    54541 ttctgcacct gatacagtct tcccatttgg acaagcagaa aatgtctggt tgccaaatgc
    54601 aaaagatatc gaagacaaag taaaagaaat cgcggaattt taagaaaaga agggacgaaa
    54661 caaagtcatg gcttatcaat ttaaattgcc ggatatcggt gaaggaatcg cagaaggtga
    54721 aatcgtcaaa tggttcgtca aaccaggaga tacgatcaat gaagatgata cattactaga
    54781 agttcaaaat gacaaatcag tagaagaaat tccgtcacct gtcacaggga cagttaaaaa
    54841 cgtgattgtt ccagaaggca ctgtagcaaa tgttggggat gtattagtag aaatcgatgc
    54901 tcctggacac aatagtgcat cgtcaactag tgcgccatca gctgaagcac caaaagaaaa
    54961 agtggaaaca tcaggttcag caagtgtcgt tgaagctgcc gatccaaata aacgtgtatt
    55021 ggctatgcca tctgttcgtc aatttgcccg tgaaaaagat gtagatatct cacaagtaac
    55081 tgcaactgga aaaggtggca gagtaacaaa agaggacatc gaaaacttct tggcaggtgg
    55141 tccaagcagt gcaccagcga aatcagaagc gccagaagcg gcagcaccga aagaagcagc
    55201 tcctgcagca gaaagcaaac cagctgcacc agcaaaacca ttcaagtcca acttaggaga
    55261 cttggaagaa cgtgtagcaa tgacgccaac acgtaaagct atcgctaaag caatggtcaa
    55321 cagtaagcat acagctccac acgtgacatt gcatgatgaa gtagaagtgt ctaaactatg
    55381 ggataatcgg aaacgattta aagaagttgc tgctgcaaat ggtacgaaac ttactttctt
    55441 gccatatgtc gtgaaagctt taacagcgac tgttaagaaa tacccagtat tgaatgcatc
    55501 tatcgacgat gcaaaccaag aaatcgttta taaacattac tacaatatcg gtattgcaac
    55561 tgatacggat cacggactat atgtaccaaa tgtgaaagat gcagaccgca aaggaatgtt
    55621 tgctattgca gacgaaatca atgagaaagc aaaattagct catgacggca aactatctgc
    55681 agaagatatg cgcaatggaa caattacgat cagcaatatc ggatctgtag gtggcggttg
    55741 gtttacacca gtcatcaact atccagaagt agctatttta ggtgtaggaa cgattgcaca
    55801 acaaccaatc gtaaatgctg aaggcgaaat cgttgtcggt cgtgtgatga aactgtcatt
    55861 aagtttcgat caccgtatcg tagatggagc aactgcgcaa caagcaatga ataatatcaa
    55921 acgtctttta gctgatccag agctattaat gatggaagga tgataaacaa atggtagttg
    55981 gagattttgc tgtagaatta gatacagttg taattggtgc gggacctggt ggctatgtag
    56041 ctgccatccg cgctgctgaa atgggacaaa aagtagcaat cattgaaaga gagtatattg
    56101 gcggtgtttg tttgaatgtc ggctgtattc cttcaaaagc actgatagct gcaggacacc
    56161 attatcaaga gtcactagat tctgaaatgt ttggagtaac tagtgaaaac gtcaaactag
    56221 attttgcaaa aacacaagaa tggaaagaaa acaaagttgt ccatacgttg acttctggtg
    56281 ttggtttcct attaaaaaaa cataaggttg aaacaatcga aggagaagcg ttcttcgtag
    56341 atgatcacac actacgcgtc attcatccag attcagctca aacttattcg ttcaataacg
    56401 caatcatcgc aacaggttcc cgtccaatcg agattccagg atttaaattc ggaggacgtg
    56461 tgttagattc tactggcgga ttagcattga aagaagtacc taagaaattt gtgatcatcg
    56521 gtggcggtgt gattggtgcc gaattgggcg gagcgtacgc aaacttagga gcagaagtaa
    56581 cgattcttga aggctcacca caaattttac caacttacga aaaagatcta gtaaaattag
    56641 tagaagatga tttcaagaaa aaaggcgtaa cagtcgtaac gaatgcaatg gcgaaagaag
    56701 cagtcgataa cggtgacagc gtgacagtga aatatgcagt ggacgggaaa gaagaatccg
    56761 ttactgctga ttatgtgatg gtcactgtag gtcgtcgtcc gaatacagat gatatgggct
    56821 tagaacaagc gggtgtcgaa gtaggagaac gaggattgat cacggtggat aaacaaggac
    56881 gtacgaacgt acctaacatc tatgccatcg gtgatatcgt tccaggggcg gcattagcac
    56941 acaaagccag ttatgaagca aaaatcgctg ctgaagcaat ctctggcaaa aaagtagctg
    57001 tagattataa agcaatgccg gcagtagcct tcactgatcc tgaattggct tctgtaggta
    57061 tgacgatcaa agaagcaaaa gatgctggta tcgaagcaaa agcatataaa ttcccatttt
    57121 cagggaacgg tcgggcatta tcgcttggta aaacagaagg attcatccgt ctagttacga
    57181 cgatcgaaga taacgtgctt attggcgcgc aaatcggtgg agtcggcgca agtgatatgg
    57241 tctctgaatt agctttagca atcgaatcag gaatgaatgc tgaagatatc gccttgacga
    57301 ttcatcctca tccatctctt ggcgaaatcg tcatggatgc ttcggaatta gcattaggtt
    57361 taccaatcca tatttgattt gaataataca ggcgaaacgc aactgaaaaa tcagttgcgt
    57421 tttgtttttt agaattatca ataatattat tatgtaaact taaaaatatg acaacttctt
    57481 ggtttattac cacttcctat gctacactga agaggaatat gaaataggag agttgaagat
    57541 gcaagaatat cagtatccac ttgatttaga ttggacaaca gacgaaatgg tcattgtaat
    57601 gaagatgtgg gaagatctgg aacgtgcgaa tgaaaaaggg ataaacaatg aagctttttt
    57661 gaagacttat cagcagttta aaactgttgt caaatccatt ggtgaggaaa aacgattggg
    57721 acgggaattt gaaaaagctt ccggttattc tttatatcgt acagtgcagc aagcaaaaaa
    57781 aaatccaaac aaaactttga gaatgaacgg gtgagataaa tggaacagat gattgatcat
    57841 atcaagtcct ggcttgaaga agctggtgag aaaatcaaat cttctcaaca aacattaaca
    57901 atctcgcaaa aatcaaatcg aacggatcta gtaacaaatg tcgatcgaaa catccagaat
    57961 tttctaatcg aaaaaatcca ttcctttgat ccagaggcga aaatattggc agaagaagaa
    58021 gggtgtaatc aattatcgga tctcgatgga agagtcttta tcatcgatcc tattgatggc
    58081 accttgaatt ttgtgattga aggggaaaat ttttgtatta tgctcgctgt ttatgaatcc
    58141 ggcgttggaa agcttggttt catttatgac gtgatgagaa atgaactttt ttggggtgga
    58201 aaaggaatag gcgtgtataa aaatgatgag ccattgccta gaccagcaaa taaatcctta
    58261 tcggaaggct tacttggtgt gaatagttat cttttcggtc ataataaata tggaatccga
    58321 gaaattgcgg aaagcagcat ggggacacgt atgtacggat gcgctggtct tgaactagtg
    58381 gcaatcttga aaggaaacca cattgcttat atttcaaacc ttagcccttg ggattatgca
    58441 gcgggaaatg tcttattgga agagtttggt atggtaacaa gcggtctttt tggtactcct
    58501 ttgacatttg aaaaaaggga atactattta gcagggactt cttgtgcata tgcagaaatc
    58561 gcagaccttt tgaaaaaaga aggctgaaaa aaaggtagaa atcattcaca aaaatgataa
    58621 aaacgagcaa aagattaatt ttctcgaaaa aatgaaaaca ttttactata tttcatgaaa
    58681 aaagtctgct ataattagca gtcgagtaaa atttgccctg tcataaagaa ccaataaaaa
    58741 aggagttagt tacattgaat tatagaaacg atatccgtaa cgtcgcaatt attgcccacg
    58801 ttgaccacgg aaaaacaaca ttagttgatg aactattaaa acaatcagat acattagacg
    58861 cacatacgca attgcaagaa cgtgcgatgg actcaaatgc gctagaaaaa gaacgtggta
    58921 tcacgatttt agccaaaaac acagctgtag actataaagg tattcgcgta aacatcatgg
    58981 atactccagg acacgcggac ttcggtggcg aagtagaacg gatcatgaaa atggttgacg
    59041 gcgttgtgtt ggtcgtagat gcgtatgaag gaacaatgcc tcaaacacgt tttgtattga
    59101 aaaaagcact ggaacaacat atcacaccaa tcgttgtcgt aaacaagatt gacaaacctt
    59161 ctgctcgtcc tgagcatgtc gtagatgaag ttcttgaact attcatcgaa ttaggagcag
    59221 atgatgatca gttagacttt cctgtgattt atgcatctgc actaaatggt acgtcaagct
    59281 tgtcagatga tccagcagat caagaaccaa ccatggcacc tatttttgat acaattattg
    59341 aaaaaatccc tgctcctgtc gataacagcg acgaaccatt acaattccaa gtttcattac
    59401 ttgactacaa cgattatgta ggtcgtatcg gaatcggacg tgtatttaga ggaacaatca
    59461 aagtaggaga ccaagtagca ttaattaaat tagacggaac agttaagaaa ttccgtgtaa
    59521 cgaaattatt tggtttcttt ggactaaaac gtctagagat tcaagaagca aaagctggag
    59581 acttgattgc tgtctcaggt atggaagata tctttgtcgg tgaaacagtt actcctgtgg
    59641 atcatcaaga tgcgttacct atccttcaca ttgacgaacc aacattgcaa atgactttct
    59701 tagttaataa ttcaccattt gccggtcgag aaggtaaatt cgtaaccgct cgtaagattg
    59761 aagaacgttt gatggcagaa cttcaaacag atgtatcctt acgagttgaa ccaacaaact
    59821 ctccagatgc atggacagtt tctggtcgtg gagaattgca tttgtctatc ttgattgaaa
    59881 acatgcgccg tgagggatac gaattgcaag tatctcgtcc agaagttatt gaaaaagaaa
    59941 tcgacggtgt aaaatgcgaa ccgtttgaac gcgtgcaaat cgatacacca gaagaatata
    60001 tgggtagtgt tatcgagtcc ttgagcttac gtaaaggtga aatgcaagat atggtccata
    60061 caggaaacgg acaaatccgt ttgacattct taacacctgc ccgcggtttg attggttatt
    60121 caactgaatt cctttcaatg acacgtggtt acggaatcat gaaccatact tttgaccaat
    60181 atctgccaat gcttcctggt caaattggcg gacgtcacca aggggcgctt gtttcgatcg
    60241 atacaggaaa agcaacgact tattctatca tgagtatcga agaacgcggt acagtcttcg
    60301 tagaaccagg tacagaagta tatgaaggaa tgattatcgg ggaaaacagc cgtgataatg
    60361 atttgactgt caacatcacg aaagcaaaac aaatgacaaa cgtacggtct gctactaaag
    60421 accaaacatc tgtcatcaaa aaaccaaaac aattgacgtt ggaagaatct ttggaattct
    60481 tgaatgatga tgaatattgc gaagtaacac cagaaagcat tcgcttgaga aaacaaattt
    60541 tagagaaaaa tgctcgtgaa aaagcatcta aaaagaaaaa ataatctgta aaaggatgaa
    60601 gagagaaacc agcttgttat aaaaaatagc tggtttcttt ttttgtccaa ctcttttctg
    60661 tggagaagtc atctttgtca atggacataa attcagaaaa tacgccgaaa gacgtttaaa
    60721 agacttttaa aaaacttatt tctagagagt ttttgtttca taataagata atgtttcacg
    60781 aaaggagatc aacttgtcct tcgaagaaaa tcgaagtaaa atggcaagag aaaggagcgg
    60841 gaagatgaac gacttattaa aacaatactt tggttatgat gagtttagac ctggtcaaaa
    60901 agaaattatc cagaaagtca ttgatcagga aaatgtatta ggaatcatgc ctaccggaag
    60961 tgggaaatca atctgttacc agcttccagc tttactatta gatgggctta ctgtggttgt
    61021 gtccccttta atctctttga tgaaggatca ggtggacgcg gcgaatcaat taggtatacc
    61081 cgccactttt atcaatagct cgttagatgg ttacgaaaca gctagacgct tccaagaaat
    61141 cgatagacag cagtaccgtt tactttatat cgctcctgaa cgttttatca tgccggattt
    61201 tatccaagca atgaagcgat ggaacgtccg tatgatcgct attgacgagg cacattgtat
    61261 ctctcagtgg gggcatgatt tccgcccaag ttatttacag atggcgaacc agctagatca
    61321 actgccaaat cgtcctgtaa ttgttgcttt gacagctaca gcgactgtgc aagtagctgc
    61381 tgacataaaa agacttttaa aaattccaga aaacaaccat attcagactg gttttgaacg
    61441 agaaaatcta cgatttcaag taattaaaga tcaaaaaaaa gaacaatatc taatcgaata
    61501 tttaaagatc aacaaaaatc aatctggtat catttatgca gcaacgagaa aagaagtaga
    61561 ccgtctctat catttgctta aaaaattcga tttttccgtg ggtagatatc atggtggtct
    61621 aaatgagaat gagcgaacag aaatgcaaga agccttttta tacgatcgct tgcagctgat
    61681 tgtagcgaca aatgcatttg gtatgggtat aaataaaagc aatgtccgtt ttgttatcca
    61741 ttatcaaatt cctggctctc tagaagcata ctatcaggaa gctggtcggg cgggaagaga
    61801 tggtctttcc agtgaagcca tcctgctttt ctctcctcaa gatatccaag tccaaaagtt
    61861 ttttatccag cagtcccaaa gagaagaagg gcaaaagcaa aaagaatatg aaaaattaaa
    61921 agcgatgaca gaatacgtgt atattgagtc ctgtcttcag caatacatcc taaactattt
    61981 tggcgagaca agcagtccgt gcaaccgttg tggtaactgt ttagatgaca gagaaatagt
    62041 agaagtaacg acagaagcac agatggttct ttcttgctta aaacgtatgg gtgagaacta
    62101 tggtaaacaa atgctgatga aagtcttagc aggatcgaaa gaacaaaagc tgcaagcttt
    62161 aggttttgga catttatcta cctatggact gatgaaaaac caatcgcaaa aagaaacgat
    62221 gcagttaatt gaatatttga tatcgaatgg ctatcttctg accatcaatg gtgagtatcc
    62281 tgttttgaaa gtaacagaaa gagggataca agtattgaaa ggacaagaat ctgtttatcg
    62341 aaaggaaccc aagaaagtcc agcagctatc ggatgaagaa accgatacac tatttgaagt
    62401 gctgcgggaa ctgcgaacgg atctagcatc agaagcaggc gttcccccgt atgtcgtatt
    62461 ttcagattcc acattgaaag aaatgagccg aatacgtcca tcaagcagat tggagatgct
    62521 tcagataaaa ggagttggcc aaagcaagct tgataaatat ggcgaagcct ttttatctcg
    62581 gataaaaaat gcggactcta acgttctgga taagtagaaa gaacgaatac gtaaaaatga
    62641 ataaaaacaa ctggttaaat ctgttttttt tgctccttat gaaaatcaaa tatgttataa
    62701 ttaaggaatt agaatgggag gtaagcacag atggattctt tgtcgaaaaa acttgcgatc
    62761 caagtattaa atgatgaagc agaacggatc aaacgattaa tccgaaatca aaacaacagt
    62821 ctctgtatct cacaatgcaa agccttcgaa gaagtggtag atacacagat gtacggcttt
    62881 tcccggcaag tcggttttgc tgtccgtata ggagtaatcg gaaagaacga gggccagctg
    62941 atgttaagtg agctagaacg agaattgaat cagttatata gtgaggttta ccaagaaatt
    63001 tatgataaaa aagaagttgg caaggaggaa tagacttgcc taagaaagtg aaaaaaaggc
    63061 atctattaga ctatagcatc ttgattccct atttaatttt atgtgtattg ggattgatca
    63121 tggtatatag ttctacgtcg tatctattat tagaaaacgg acaaaatccg tccgcatcag
    63181 tcattaacca gagtattttt tgggtgctta gcttgattgc tatcgcacta ctttataaaa
    63241 tgaaaacaga tgttttgaaa aaccagcgtt tgatcatggc agccatcgca gttttgacga
    63301 ttttgctgct gattgttgtg ttttttggta aggagatcaa tggggcgaaa ggttggctgc
    63361 aaattgcagg attctcgatc caacctgccg aatacctaaa aatcatttct atctggtacc
    63421 tgtctctgac tttatctaaa agacaaaaca gtgtacaaaa agattttctt gggactgtga
    63481 aacgtccact tgcaatggtt atcggactta ctgcattagt cgctattctt cccgattttg
    63541 gtaatgcggc agttatcttt ttgattattt tggttctgct tttagccagc ggagtcaatt
    63601 atgtgtatac tttgattgta ggagtaggag gcttttgtct aagtactttt acgatctggc
    63661 tgatcaatat cacaaacgga aagatcttac ccggacgttt acagtatatc tataatcgat
    63721 ttgctattta ccaaaatcct ttctcagacg aattaaataa agggcatcaa ctagtcaacg
    63781 gctactatgc catgttcaac ggaggactat ttggcagggg attaggtaac agtatccaga
    63841 aaaaaggatt tttacaagaa gcacaaaccg attttattta tgcaatcgta gtggaagaac
    63901 tgggagtgat tatggggatt ttgatattag cgttgctatt ctttatgatt gtgcgcattg
    63961 tccttgtagg tatccgttca aaagatccgt tcaattcgct tttgtgcatt gggatcggcg
    64021 caatgttttt gatccaagta tttgtcaatc ttggaggaat tactggcgtt attcctttga
    64081 cagggatcac gttcccattt ttaagtcaag gaggctccag tttgctgatg ctttcgattt
    64141 gtgtcggctt tgtcctcaac atcagtgcgg atgaaaaacg caagagctta ggtttataat
    64201 aaaaaaagat gtaggagtgg ttcgatgaaa aaagttttag ttgctaacag gggtgaaatc
    64261 gctgtacgtg tatttcgtgc ttgtacagaa ttaggaatta aaactgttgg aatctatgca
    64321 gaagaagatg aatattccgt acaccgtttc aaagcggatg aagcatattt agtaggacaa
    64381 ggaaaaaaac cgattgatgc gtatctagat attgaaggga tcatttctat cgcgaaagaa
    64441 tgcggagctg acgctattca tccgggttac ggtcttttgt cggaaaactt gaattttgcg
    64501 caacggtgcg aagaagaagg catcattttt gttggaccca aactgcatca tttagatatt
    64561 tttggagata aaatcaaagc aaaagctgca gctatcgaag cggggattgc ttcgattcca
    64621 ggcacagatg gaccaatcgc ttctatagac gatgccttgg agtttgcgaa acaatacggt
    64681 tatccgatta tgatcaaggc agctctaggc ggcggcggac gcggcatgcg tgtagctcac
    64741 gatgaaaaga gtgcaagaga gggctacgaa cgtgcgaaaa gcgaagcaaa agcagccttt
    64801 ggtagtgatg aagtgtatgt tgaaaaatac atcgctaatc ctaagcatat cgaagtacag
    64861 attctcgggg atacgcatgg aaacgtcatc catctttttg aaagagattg ttctgttcag
    64921 cgccgtcacc aaaaagtagt agaagtagcg ccttgtgtct ctatgaatga acagcaacgt
    64981 caaaaaatct gccaggcagc tgtccaattg atgaaatatg tcgggtatgt caacgcaggt
    65041 acagtagaat ttcttgttga aggagatgat ttttacttta tcgaagtcaa ccctcgcgtc
    65101 caagtggaac atacgatcac agaaatgatt acagatattg atatcgtaac aacgcaactg
    65161 ttgattgctc aaggtttgga tctgcataaa gaaatcggat tgcctcagca agaaggaatt
    65221 aaattaaatg gttcagccat tcaatgccga atcactacag aagacccgtt gaataacttt
    65281 ttaccagaca cagggaaaat cgatacgtat cgttcacctg gtggttttgg cgtacgtctg
    65341 gatgtcggaa atgcttacgc gggctatgta gtgacaccgt attttgattc attgctagta
    65401 aaagtatgta cgcatggtgc tacctttgaa acagccattc agaaaatgga acgttgcttg
    65461 aaagaattta gaatccgtgg agtgaaaaca aacattccat ttatgctcaa tgtcatcaca
    65521 catcctgaat tccaatcagg aaatgcaaaa acaaccttca ttgatagtac agcaacatta
    65581 tttgaatttc ctcgactacg agaccgcgga aacaaaacga tgaaatatat cggtgaaatt
    65641 actgtgaatg gttttccagg tatcgaaagt ggcgaaaaac cattttatga agaaccacgt
    65701 atgcctaaag atctaatcac aagaactgat tacgtaacgg ctaagaatgt attagatgcg
    65761 aagggtgccg atgctttggt cgaatggatc aaaggacagg aaaatttgtt gttgacggat
    65821 acgacattta gagacgccca tcaaagtctg ctagcaactc gtgttcggac aaaggatttc
    65881 aagcagattg cacgtttgac tggagaaggt cttcctgaac tcttttcaag tgagatgtgg
    65941 gggggagcaa catttgatgt ggcttaccgc tttttgaatg aagatccttg gcaacggttg
    66001 agaaaaattc gttctttaat gcctaatacg ttgctgcaaa tgcttttcag aggttcaaat
    66061 gccgttgggt attctaatta tcctgacaat gtattggtgg aatttgtcaa agaagcagca
    66121 gcgcaaggaa tcgatgtatt tcgaatcttt gatagtttga actggactcc acaaatggaa
    66181 aaaagtatcc aagctgtacg ggatactggt aaaatcgcag aggcagctat ttgctatact
    66241 ggcgatatca acgatccaag tcgggcaaaa tacaatgttc agtattataa agacatggca
    66301 aaggaactag aacaactggg tgcgcatatc attgcaatca aagatatggc tgggctgctt
    66361 aaaccgcagg cagcttatcg tttgattagt gaattgaaag aaacgacaga tctgccaatc
    66421 catctgcata ctcatgatac cagcgggaat ggaatcatca cttattctgc tgcttcaaaa
    66481 gcaggagtag acatcgttga cgtggcaatg agtgcaatga gtgggaatac tagtcagcct
    66541 agcatgagca gcttgtatta tgcactggtc aatggtccac ggctgccaga aatcacgatt
    66601 gaaaatgcac agaaattaaa ccattattgg gaagatgtgc gcatgtatta caaaccgttt
    66661 gaaaacggct tgaatgctcc tgaaacagaa gtatatatgc atgaaatgcc aggaggacag
    66721 tattcgaatc tacagcaaca ggcaaaagct gtcggattag gtcatcgctg ggatgaaatc
    66781 aaacaaatgt atcatactgt caacttgatg tttggcgata tcgttaaagt aactccatca
    66841 tctaaagtag ttggagatat ggcactattt atggtccaaa atgatcttac agaagaagat
    66901 atttatgaaa aaggcgaaac actaagtttc cctgaatctg ttgtgacttt cttccaagga
    66961 gaactagggc aacccgttgg cggatttcct aaaaaattgc aaaagattat tcttaaaggc
    67021 agacctgcat tgaatgaacg tccaggtctt ttggctgaat cagtcgactt caacgaagta
    67081 aaaaaagaat tggcagaaaa aattggctat gaaccaaaac aagacgaagt attaagctat
    67141 ttgatgtatc cgcaagtctt tttggactat caaaaagcat ataatcaatt tggcgatgtt
    67201 acattactgg acacaccaac atttttccaa ggtatacgtt tgggtgaaac aatcaatgta
    67261 caaatcgaac gcggaaaaac gcttatcatt cgtttagatg aaatcggaga acccgatatc
    67321 gaagggaatc gcgtactgtt cttcaatttg aatggacaaa gacgtgaaat cactatcaaa
    67381 gataattcga tcatcagtac ggtccaaacc cgattgaaag ctgaaccgac aaaccgagaa
    67441 caaattggag caacgatgtc tggttctgtt ttggatgtat tagtgaaaaa aggcgataac
    67501 gttaaaaaag gtgatacgtt gatgattact gaagcgatga aaatggaaac ggccatcgaa
    67561 gcacgttttg acggagaagt cgcacatgta tatgtgtctt caggagacac tatttcgtct
    67621 ggtgaccttt tgattgaagt gactgagaaa taataagcga aaggtgggga aacatgaaaa
    67681 gactgggttt gttttggctg attctgctat tagttattgt ggcattctac atggaaccag
    67741 tcttgtttcc gccaaaactt gaagtgaagg aaaactctga tcaacaaata tcaaaccaaa
    67801 tgacaacgtg gaaataccag gagcttaaag caactggttt ttctacttat atcggaaaac
    67861 cgatcgatca gctggaaaag gattttggcc aagcctatga tcgattaact agcggatttg
    67921 gttttgaaac aagatattat gaaaataaag ctagtcatct cacatttgaa gcgaacatcc
    67981 aagacagcag agtcacaact gttaaagtac tgcaaacagg gcctgctgat atcgcgccgt
    68041 tttatttagg gatgacgatg caagatctta cgaaaatcac tactatctat actaatttca
    68101 cttttgagta ccatgataca gaagttggaa tcgaactaat ggaagaggat atgaattatc
    68161 gtccgctaat tgcttttgat aacgacactt ttgctatttt attttttgat caaacaacaa
    68221 acggcctatt ttcagttgtg tatttagata aagatagtct tttgaaacta ttgccatatc
    68281 aagtgtttgg tgaaggcctt ccacattatc aagcagataa atcggctgat tgggaatcaa
    68341 tcaatcgcgt taaagaacga aaaagtacga ctttattaaa tatgcttaga agagaagacg
    68401 aacttcccat atacaatcaa acacttgcct ttcaaaacca cagtcagctg ctactacatg
    68461 attttttgac aaggcctgag gaaatattgt cagaggaacg tatggctgat tggcaaaaat
    68521 cgctaggcag tgctcaaacg acagtgaaat tctcactgac gaatgatgaa ttggagaagt
    68581 taatcagtaa acaaaaatta gaaaatacta gtgggctatt tattcatccg gcgattgacg
    68641 ctactttttc gtttttatat tggtacagcg atccttacaa ccatgaccgt ttcatggacc
    68701 cgggaacgga ttctgtaggc attgcttttt ctaaagaaaa tatgttagtc ttattgcaag
    68761 aagaaaacgg agaaagtagt gatagcagtg atcgttaacg aagagctgtt tcaactggag
    68821 gatcaatgcc tccaagtcgc tgaaatgata aaaaagagta aaacgatggc ttcttatctt
    68881 gaacataaac agtcaatgga agaatctgca aaagtttgcc agttgaaaga agcgttcttg
    68941 tctaagaaag aagcatttga aaatgtagcg gcatatggtg agtatgcacc tgattatcgt
    69001 gaaaaaaaac gagctgttcg aaaagcaaaa agacagcttg atatggatga gcatgttgca
    69061 gctttccgat atagtgaaac acagctgcaa agtctgttgg atcaaattgt ccaagtactt
    69121 gccgaagaag tttctaatga aatcaaagta gatgcaggta gcccgttttt tgaaacgaaa
    69181 aaacactttg gatgcggagg taattgtcat gcaattagac aatagtgaat ttgaaataac
    69241 aaaacgacgc gggctgatcg tatgggtcta cagcttaaaa caattaaaga atctgaaaaa
    69301 gttcggcttg ttgcattatg tctcaagaag aatgaaatat gtcgtgcttt acttaaatga
    69361 agagacattg gaagaaacag aagaacgaat ccgtaaatta cattttgttc gaaacgtaga
    69421 acgttcttac cgaccggata tcgaaatgaa ttttgccgaa aaaataggaa acaaaacagt
    69481 attaaaagac gaaggtttcg aaattgaaga gctaaataca gaaatccgat tagctgaaca
    69541 tctttaaaaa tctcttatga gcagttttta aataaaaaat aacttggata acgatcgatt
    69601 tttgatcatt gtccaagtat ttttttggta tactagtacc agtaccattt ctaaggagtg
    69661 agtagatgcg gataatatca ggaaattatg gcgggcgccg tttaaaaagt ttagcagggg
    69721 cgaacaccag accaactaca gataaagtaa aagaatccat attcaatatg attggaccat
    69781 attttgatgg agagacagta ttggatctgt ttgcaggaag cggtggttta gctatcgaag
    69841 cagtatctag aggctgcagt catgcggtat gcgtagacaa aaattaccaa gcgctaaaaa
    69901 tcatcaaaga aaacatcgaa attacgaaag aaccagagaa atttaccaca ataaaacttg
    69961 atgcggacaa agcaatcgaa cggctagcag cggagaaaca acaattcgac tacttgtttc
    70021 ttgatccgcc atatgcaaaa caaaaaataa tcgatcaaat ccataaaatg gaagaactgg
    70081 gacttttttc taaaaccgca gttatcgtat gcgaaacgga taaaacagtc gaattgccag
    70141 aaactgtggc tggattccat caaataagaa aacagattta tggcataacg gccgtaacta
    70201 tctatagaaa agagggaaat caatgaaacg tgcattattc ccaggaagtt ttgatccttt
    70261 tacaaaaggt cacttagata cggtggaacg tgcggctaaa ttattcgatg aggtagtaat
    70321 tggtgttttt atcaatactt caaagaaaag tttgtttccc ccagaagaaa gaatgacatt
    70381 gatcacaaaa gctgtttctc atcttcctaa tgtaaaagtc atgcatcaag aaaatcaatt
    70441 gacggtagaa acagcaaaag agattggtgc agatgcgttg atcagaggca tacgaagtat
    70501 caaagacttt gaatatgaac gagagattgc tcagatgaat catcatctat atgatgaatt
    70561 ggaaactgtc ttcttattag caaaaccaga atatagtcac gtgagttcca gtattttaaa
    70621 agaagtactg cactttggcg gagatgtgag cagctactta ccgccagtga tcaacgaagc
    70681 tttagctgga aagcgtgaat caaatgaaag ctaaacaatg gttgaaaaca gtactgatta
    70741 tcatatttgg aatgttgatc attggatttg tagtagttcc cttgccgtat tatgtagaat
    70801 caccgggagc cacgatcgat ctgaaagaac taattacggt caatgataag gaagataaag
    70861 gttctgggtc tttctctctt acctctgttg gtatcaggca agctacaggt ttcttagctt
    70921 taaaagctaa aatgacccct tttgaagaat tgatcagtga agaagaattg actggtgggg
    70981 cgacaaatga agaatataat caaatgcaac agtattatat ggattcttcc caaaatgcag
    71041 caatcgaaca agcgttaaaa cttgcagaca agccttataa aatggaattc aaaggcgtat
    71101 atgtcatgag tgtagagaaa acatcgaatt tctttggcaa gctttcagtt ggagatacag
    71161 taacaaaagt agacgggaaa agctttcaaa gtacagaagc atttatggat tatgtaaaaa
    71221 gccaaaaagt tggacaaaca atcgaaatca gttatttgca taatggtgaa gaaaaaacag
    71281 cttctggtga tctgatcgag ttaccaacag ataaaaaggc aggaataggt atcacgctta
    71341 ctgaccatac cgagatcgaa tcagatacag atgttcgtat taattctggc tctatcggtg
    71401 ggccatcagc gggattgatg ttcactttag aaatctatga acaagtcact ggtaaaaact
    71461 tgcgacatgg taagcaaatt gcaggaactg gaacgatcaa cagtgaagga gaagttggcc
    71521 gaatcggagg aatcgataaa aaagtggcaa gtgcggataa agctggcgta gagattttct
    71581 ttgcaccaga tgatacgatt tcagacgatg ttaagaaaga atatcctgaa ataaaaacca
    71641 actacgaaga agcaaaagct gccgctaaaa aattgaattc ctcaatgaaa atcgtaccag
    71701 taaaaacagt acaagaagcg cttgactatc tagagaaaat gaaatgaaac taaataatta
    71761 aagaggggtt gtgacacgag ttctgtcaca acccctcttt ttcgtcaaaa atcttcacga
    71821 taaaaataaa aacaaaagaa aaaattgacg taattagatg ttgaagggac gattttgcca
    71881 gagctcactg tttcttcaat tttaaatgaa aggagtaatg aatgattttt actggtcatt
    71941 ctttgaaaaa agaacaatga tagaaaaaat cagcaagaaa atactagctt cacccaaatt
    72001 gatcatttgg atgtctatga tagttcttat cttttgtatc gcaagtggtt ttttattatc
    72061 aaatttgcta ttttatagag aaacagaaga gactgctgca aaagaaatac tttcttcgtt
    72121 tagttccagt ggagaagatc catcagctga aggaaacgat gtagaagagg atgctcgtcc
    72181 aaaggtcatg tacaccgata tcaaaggaag tgtgaaggag cctggtatct attccttttc
    72241 ttcagaagaa cgggtctatg atgtactaaa gcgtgcaggt ggattattag aagaagcgga
    72301 tagcgatcga atcaactttt ctgcgaagat agaagatcag caagtcctat atatccctgc
    72361 tgtaggtgaa gagctgccag aacatcttga tcagttggca tcctcggaag gaaaacagcc
    72421 tacagcagat tccgagcctc ccaaaataaa tatcaataca gccactccat cagaacttca
    72481 gcagattcct gggatcggat cagtcaaagc gcaagaaatc attcgattta gagaagaaaa
    72541 cggatccttc cagaaagtgg aagacctgca ggagatttct ggtatcggtg aaaagacagt
    72601 tgaaaaacta aaaaacttcg ttacaattaa atagaatgat tcaaaatcga ctggcacgag
    72661 caataaatgg acgctaatgg atactaagaa taatccaatt ctaagagctt ggcaggacga
    72721 ggaggtagta tttttggata ggaaatagct tgattgttcg caaagaaaca ttaaaaaaac
    72781 caatgctgaa tgtctatctc caaaataaaa tatctgggat tcatattatg aatacggctg
    72841 tcagcggcaa taacagtcaa gctctacgag aacgctttgc caaagatgta cttagctata
    72901 cagcagataa agtatttatt ctcattggga cgaatgatct agcagagcat aaacaacttt
    72961 ctaaagaaac gtaccaaaaa atctgcagtg gatgatagtt cagctgaaaa aagactatag
    73021 ttctgatcat gctttcttta tcacgccacc agcagtcgat gagacaaaac aaaaatatcg
    73081 gaataacaag ttgatcaaag aatatatctc tgttctcaca gaagtagcaa aacgccatga
    73141 ttgtgaagta tcgattttta tacggaattt cttgaacaaa tggattcgaa gaatttttat
    73201 aatgggctaa tggatgacgg acttcatttc ggcagtgaag ggtaggatct tttgtcagac
    73261 aggatgattc gttatattta aagaatattt tgtttaagca atgagtactt tttaatcaac
    73321 ttgaaaaaac ggaggaatat aaaacatgtt agaaaaatct tcaaaagcat gcgatacgaa
    73381 taaagaaaaa gttactgata agtcacataa tgaacgcatt ccttgggacc agtactttat
    73441 ggctcaggca gtactgttat ctttacgtag tacgtgtaca cgtttagaag taggagcgac
    73501 actcgttcga gaaaaaagga tcattgctgg cggctataat ggagcagtca gtggagatgt
    73561 ccactgcata gatgaagggt gttacgtcgt ggatggacac tgcttgcgaa cgatccatgc
    73621 agagatgaat gcactcctac agtgtgcaaa acttggtatc ccgacggaag gttcagaaat
    73681 ctatgtcaca cattttccat gtttggcctg tacaaaggct ttgttgcaag ctggtgtgaa
    73741 agggatcaat tatctccatg actatcgaaa tgatccttat gcccaagcgt tgatcgaaca
    73801 attaggtgta tctgtacacc aagttgcttt agactcacag catttttcaa aacttcaaaa
    73861 tgaattaaca ggaaaacatg tctagcgttt tagaaaggca tgttcgaaat cggttgattt
    73921 ttccggcaat tacgtcgatg attatctgtt tgttgtatta cactgacaat tggggttatc
    73981 gtttgcttct tgccggaatt tttttatggt tttcgctaaa agaaggacgt tttatcgcgt
    74041 acttgtgtat tttgtgtagc ataacggtat ccatttctta taggctacaa gagtattcat
    74101 ttttagagga aaaaacaaca tctgcgcaaa ttactgtatt tacagatacg attcgtgcaa
    74161 atggtaattt tttgacaatg gaaggaaggt tatcagagaa taaacaaaag ctccagcttt
    74221 cttatcaagt aaacacagaa gcagaattga aaacatggct taacttccga ggaaagggaa
    74281 tagaattgac tgctgaaggc gagttcatct ctccagcaaa aaagcggaat ctttataatt
    74341 ttgaccaaga agcttacttc aggaaccata gaatttctgg acggttccaa attcaaaaga
    74401 ttgaacagat caccatgcaa aatcactggc gaaactggct tcagagaaaa agaggatctt
    74461 ttatctcctt tgtccaatct gaatttccgc aaaaaacaag tgtatatatt aattctttat
    74521 tatggggcta taaagatgca ggattcagag aaccagaaga aatttttagg gagagtggtt
    74581 tgctgcatct gttcagtcta tcgggtctac acatccaatt ttatttaggg tggctgtatt
    74641 atttatttcg acgtatcgga tggccactac acgtcagtat tattccgttg agtctactaa
    74701 ctatctgtta tgtttctatt gcaggaagtt ctatttctgt tttgcgtgct agtttgctgt
    74761 ttttattgcg attgctagtc aaattgctga ataagcatta ttccaccatg gacttatttg
    74821 cagttgtctt ttggcttctt ttactatttg atcctcgatt tctttttcat gcgggtggac
    74881 agcttagttt tttcatgagt tttttgttta tcctgatttc ttttgatgga gattggttga
    74941 aaaaaacggc ctctcacgtc ctgtttacta tgatcacgat gccgttgatt gtatggcatt
    75001 tttatgagtg gcctatttta ggtagtcttt ttacattact gattgcaccg gtattcaagt
    75061 ttctttttct tcctggtgtg ttatttctca cactcttcaa tcgctggctg ccggaaactt
    75121 tacttttgct gatagaagag ggattaatct tatttgagcg aataatcgat ttttcttcag
    75181 ggtcgtctct cattatagga cgtttgcccc ttgtgctatc tgggatgtat attttcggat
    75241 tactgatttg tatggatagg ttgaaagaac aacctgtgaa acacttgttt tatgttggtc
    75301 ttttttcttt tatttttttg actttgccat ttttacgctt ttcttcaacg atcgctttta
    75361 tcgatgtcgg tcaaggagac agtatctttt tgcaaagtcc ttttcgaaaa gaaacgattt
    75421 tgatcgatac tggaggtcag ttacactttg aaagagaaaa gtgggcgcaa ggtatgatac
    75481 gtcctcctgc tgaaagaaat atcgttccat atctaaaagg tcaggggata agtgtaatcg
    75541 ataaactcat cttgacacac gacgatacgg atcatgtggg agaattaccc acgttatctc
    75601 agcattttac tatcaaaaaa atttatatcg gttggggcgc tggacgaaat gagccattac
    75661 aaaggcattt atctgaagct caaaaaaatg gtactgagat cattgaaatc aaacatgggg
    75721 acaggatcag cggttattat gatctttatg tattgactcc ttttgaaaag ggagagggaa
    75781 gaaatgaaga ttcgattggt ctatggatgg aatataacaa taggcgcttt ttatttttag
    75841 gagatttgaa tcaagcaatg gaaaaacaat tgcttttgat ttatcctaat ctgaaagccg
    75901 atgtagtcaa attaggtcat cacggtagcc gtacgtcaag taacacagat tttctttccc
    75961 atattgaagc aaaacatggg attatatcct gcggcgtaaa taatcgttat ggtcacccgc
    76021 acagcgaagt attggagaca ctagaagaaa atcagataaa aacatggcga acggatcaac
    76081 aaggaatgat cacctatcgc tggcatccag tttttcatcc tcatgggcta gtggaaacaa
    76141 tgatagacta gtaattctca tctgctcatg gtaggataat ttttgaaagt ggtgagtaat
    76201 ttgagtatac aagcagagtt acaaaaaata cgtaaagaaa aacttgcatc ttgttacttg
    76261 attttaggaa cagaaaagtt tctccaagat caagttcgga cagaaattgt caaaaaaatc
    76321 caattagagg gcgaagatga cttaaatttt ttaagttttg acatggaaaa tgcaagttta
    76381 gatgaggtag tagctgaagc agagacgttg ccgtttttcg gagatcaacg gttagtattc
    76441 gtagagaatc cttatttttt aacaggtgaa aaagtaaaca atggaatcga acaaaatact
    76501 gatttattga cggcttattt gaaagaacca ttagaatcga ctgtattagt attctttgcg
    76561 ccatatgaaa agttggatga acggaaaaaa gtaacgaaac aattgaaaaa aacagcaatt
    76621 atcatcaatg tccaacagtt gaatgaaaaa gaagtacgtc aatatctgat gaatacctta
    76681 gagaattcct caatcaaaat ggaacgaagt gcaattgatt tattcttgag gttgaccgac
    76741 ttagatttat ccaaaatgat gagagagtta cagaaattgg tcctttatgg acaaaatcaa
    76801 cagcaaatca cggtaagaga agtagaagaa ttagttccta aaacgttaga acataatatt
    76861 tttgacatga cccaatatat tttaaaaggg aagacggaac aagctttgcg tttatacgaa
    76921 gatttagtta tgcaagggga agaaacaatc aaaattaatg cgattttgct ttctcaatta
    76981 cgtttatttt tacagacaaa atttcttgta aagattggtt atcagcaggc aaatattgct
    77041 gaaacattga aaatccatcc atatcgtgta aaacttgcca tgcaggaagt aaaaaaattc
    77101 gatgaacatt tgctggtaca gttgtttgat caattagttg agatggatta tcaaatcaaa
    77161 acgggacaaa tcgaaaaaga attaagtttt caactattcg ttttgcgcac agggcagttg
    77221 ttaagaaatc gataagaaga aagacgaggg caaagcatgc gggaaggtcg tttgtttttg
    77281 tccttttttt gacaaataaa aaaatcggcc gaagccgatt cttttattta gcgatttttt
    77341 tgcttaaacg agatttgtcg cggttagctt tgtttttgtg gattagacct tttgattcag
    77401 ccatatcaat agctttaaca gcttctttat ataacgcatc tacgttgtca gcacctgaag
    77461 ctacagcatc ttcaaatttt ttgattgcag tacgcatagc gcttgtttga gatgaatttt
    77521 tcacattagc gttttcgctt gtacgaacac gtttgattgc agattcaatg tttggcatta
    77581 gtttcacctc cgataactta agttcttacg cgtgttaccg cataattcaa cattcattat
    77641 tatacctaaa ggcagcgtgc tttgcaatat gaacatgaca agtttttttc cttaacagtc
    77701 ttttattagg attttttttc agtaaaaacg gatagtacaa gctgaaaaaa atcaaaaaga
    77761 cgctattgtg ggaggcaata gcgtcaaacc aaactagatt tttatttatc tatttaaaca
    77821 aagtttgaat gggaatgttt ggtactaagt tattatataa caaagagaac cgtttgcaaa
    77881 gttttttttg aaaagggata cgattccctg ttgttctttg tttttctatt aagaaactgc
    77941 agttatttta taaccagtat cctttgatca gtaaaggcta ttctggtgta ccgattattt
    78001 taatgtattc atcgatattt tttatcaaca ttttttttat gaccaactgg ttccgacggt
    78061 tccatttgcc acttacttcc agcacatatt tatttagggg caaacgtaag atatattttg
    78121 ccaaatagcg atctttaagc aggccattga tcacacggtc ctcaagctgc aagttgatcc
    78181 cagtcagcat cattggctgt tcttgtaaaa cgtacgtatc aattaaaaga ccttcaaaag
    78241 tttccacaag cgtcttcctt tcttttcaaa gtaacctgaa aacaggcact aattcttcca
    78301 ttttgatatt cgttgaagtg gatgcgatca ttctttcttt tgtccactgt aatctagtat
    78361 aacaaaaaag agcagaagag ccaaaaagaa aaaagagcat cgaattctat aggaagtctc
    78421 attggcacgg cctaaattat tacaaaacat tgattaaaaa aagcccctgc atcgcaggag
    78481 catcccctat atcgttttaa taggtcaatc aaaaagcagt atcaaacaca ttttgtatca
    78541 aatcaggaaa taatagataa gctaagccca gaatcaaaag taccgctaga agaaaccagc
    78601 cgattgttcc tatgaaatac tttaattttt cgaacattgt atcacctcgt tgtcagtata
    78661 tcacggatca gtaaattggg ggtgttaaga atctcctaaa atagacaaaa tttgttctat
    78721 tttcctaaag caaatacttg taagatacag taagtgatac cagaagcaat gaccggtccg
    78781 gcggcgattc ctcttaagaa gacaacacct aaaatcgtgc cgaaaacaag tgcaacagtg
    78841 atttgtggat cagaagcaat caatcctaca cccttggaag ataagacagc cactagccca
    78901 ccgcaaaaaa cagctaccca gccaataggg gatttaaagg cttctaatag atcacgtaga
    78961 ccgatttctc ctgttgcgat agggattaga atagccgctg tgatgatcgt caccccaata
    79021 ttgattcctt tgccttgaag ccaagggaaa aaggaacggg aggctggtat gagcttcata
    79081 atcaaaacaa aagctgccgc tatcatcaga ctttgatttt tagctaaaat tgctacaagt
    79141 aaaataattc ctaaaaaaag ccaactttcc atgaaatata ttcctcactt tcttcgaaac
    79201 aatcataaca tgtttttaga aagttacaaa agtaggaatt catccttcaa gacctattca
    79261 taagctgttt ttttcataaa ataaacaaaa tcataaaaaa acactttgct tatttgacag
    79321 aagacagccg cgctttgtat agtggacaag acttacattt ttttgaagaa tcagaaagat
    79381 caggtaaagg tcttagtaaa cgcaagccat tcagaataac caatattgtg cttccttcgt
    79441 gtcctacaac tccaagaggt agatttaaaa attgcaggaa gttagaagca atcaacaaga
    79501 gaataacaag aatcgaaaaa ataatatttt gtttgatgat ccgccgcatt tttaaagata
    79561 gccgatgact gtagacaaat ttatttaagt cgcttttcat taagacgatg tctgcgatat
    79621 ccatagcggc atctgtccct tgccccatag cgacacctaa agaagcggct gctaaagcag
    79681 gcgcatcatt gactccatca ccaaccatcg catttacttg gtaaagttca ttctcttttt
    79741 tgactagttg tgttttttct tccggagtac agcttgcata gtaatgttca atccctactt
    79801 gttcagcaat ggcctttgct gtgccagaat ggtcgccggt tatcatactt gtatggatgt
    79861 tttgggattt aaagtaggaa attacttgtt gggtatttgc ttttggaata tctagcagac
    79921 ctaaaacagc aattaactgc tgatctttag aaagataaat cacggttttg ccttgatttt
    79981 ccaaacgttc gatcatttcc gcaatctctt tgctgattat catcgtctca ggatcatatg
    80041 cgtgcttgcc aactttccat tgcgcaccta aataggtagt ttgtaatcca aaaccagtaa
    80101 tttcttctat tggtaagtat tggatttctt caggtattgt gcaatcgaaa tattgcaaaa
    80161 tcgcatgagc aagtggatgc gtcgtttttt gttccatagc aacaagaagt tgttgtgcca
    80221 aatccttttc atcaaagaaa aagctgtcag ttacaactgg ttttccttta gtgagcgttc
    80281 ctgttttatc aaaggcaatc gcttttaatt cagaaagctt ttctaaatgg ataccgcctt
    80341 tgattaaaat cccatttttt gcagaatttg aaagagcagc tagagaagct ggtgtagcag
    80401 atgcgacaag cgcacaagga gaagccacaa ctaataatac cattcctcga taaaagcttt
    80461 catcccaact ccaacctagg aaaaagtggg ggagaaggat cattagagga acagcaagca
    80521 aaatcaattt tacataatta ttttcgattc gttcgataaa tgaagctgtt tgagttggcg
    80581 tattctgcgc ttcttctact aattggataa tcttagcaaa gacagtttcg gaactcgttt
    80641 ttgttactcg aatcgtgatg ggatttcctt gattcagcgt tcctgcatac atctcatcgc
    80701 ctaaatattt ttctacaggc acagattcac cgcttaatgc agactcatcg atcgttgcct
    80761 gtgtgctttc taaaaagcca tctatcggaa cagctgctcc tttagcgaca aataccaagt
    80821 tatccacctt tagttgagaa acatctactt cttgcatttg tccattttct tttaataatt
    80881 gggctttttg tggttgaaga ttcatcaaag cagtaatttc tttttgactt ttattagttg
    80941 tgtattcctc taatgcaccg cttaaacaaa agatgaatgt caacattgct ccttcaaagt
    81001 aattaccgat caaacaggca ccaatagcag ctaaagccat caataaatcc acattcagtg
    81061 tccgatcatt ccacaattct ttcaagcctt ccgatgtttg tttatatcca ccgatgataa
    81121 tggcactgat ataaaaaagt ggggtgatgg taagagagaa tcggtcgaag ataaaaccga
    81181 agatcataaa taaaaggcag ccgattgttg ctgcaaaagc ttttttctga ttagtcatcg
    81241 ttattagacc tccttgtcaa aacaaggata gcataataaa aatgtaattg ataataataa
    81301 cagctaaatg agaacgataa tggttatcat ttggtgatta agcttgatgt tgtttttatt
    81361 atttataatg attattatta aagaaaataa aaaaccagaa catttctcaa ataagagaag
    81421 tgttctggtt ttttctacat tgcaataaac caataaccaa tcaaaatgat accaaggata
    81481 atacgatacc agccaaaagc ggtaaagtcg tttttcttca aatagcttag taagaatttg
    81541 atagctaaga cggaaaccac aaaagatacg aatgttccta caagaagaac aacagtttct
    81601 tccattttga atgaattccc ttcagctaaa tatttgacga tcttcaagcc gcttgcgcca
    81661 aacatgacag gaatccctaa gaaaaatgaa aactctgtag cgacaaaacg cgaagcacca
    81721 atcaagatag atcctaggat cgttgcgcca gaacgggatg ttccgggaac aagtgccaag
    81781 acttggaata acccaacaag aagagctgct tggtacgtaa agtcattcag agaagtccaa
    81841 cgaggtgttt tgtttttgtt gcgtttttcg acaataataa aggcaatacc gtaaacgatc
    81901 aaaacaatgg caactggtaa gaacttgtgg aaatttgctt ctaaccaatc atccaaaggc
    81961 aaaccaatga tgatggctgg tatgcaagca acgatcactt taaaccaaag tgtccacgta
    82021 tctttttttt ctaccgttga tttacttgga gaaaatgggt ttaacttgtg aaaataaaga
    82081 acgacgacag ccaagatcgc ccctaattgg ataacgacat tgaacatcga cataaattgt
    82141 gaactggcat ccagtttgat gaattcatct gctaggatca aatgaccagt actactgatg
    82201 ggcagccatt ccgttattcc ttcaataata ccgagaatca ctgctttgat aatatttgca
    82261 aaaaacataa attccttcct tcccaattaa gatacatctt atagtatacc gttatttttt
    82321 gagaaaccca atctgttttt taatattaag aaaaacttga gaaataaagc agataagtac
    82381 acgaagagaa aaaagaagac aaagccctcg ttcagacttt gccttttatg attctctggt
    82441 aaaaattaaa atatccccat tatagaaacg agtcgcgact atgtgttttc ccgttatcga
    82501 tataagacgt atcattgatg ccgtggatag aaaatcgacc gtttgcataa gtgacctttg
    82561 ttacgctgcc atttaaaagc ccagcacgaa caggctgttt aggatcgatc agattaagca
    82621 aaaaggcgat cgtcaaacca tgagatacga ctaaaacatt tccaccacca tttttttcgc
    82681 agtgatgggc gatatcttca aatcctgtcc atacacggtc tcgcaatgtt tcataagttt
    82741 ccgcccaccc agcggtatct aagttgtata cagcattagc aatttgttca aaagaaactt
    82801 ctgtggtaaa catttcatca tatgttttaa aattcaatac acgagggatc acaccccaca
    82861 tttccatgtc atatccacct tcaagagacc cgaaacacca ttctctaatc cttttttcaa
    82921 taaaataagg aatttctttt cccttagaat gttctcctag aataatacgg gcagtttcga
    82981 ttgcacgacc actgtcacta gaataagcaa atgaaaagtc gacatttttt aacccgcgac
    83041 ctaaataatg aatgccttct tgtccttgct ttgtgagggg tgtatcacac cagccttgta
    83101 ctcgttcaat tgtattgaac atcgtttttc cgtgtcgtac gatataaagt gtagtttctg
    83161 tcattttatt ctcctcctaa gtcgttttaa cgattgaaaa aaggatttgt ccttttttcc
    83221 tgttcaatcg tcgttgcatc gccatgtcct ggatagactg caaattcatc aggtaataca
    83281 aacagctggg tccgaatact atgaagtaat tgttctaaat tccctgtata cagatcggtt
    83341 ctaccgatac ttcctctaaa cagggcgtcg ccagaaacga caaaatcatc aaaaataaag
    83401 ctgacgcttc ctatagaatg ccctggggta gggacgacgc gaaaagacat gtttcctaaa
    83461 gtataagttt tcatttcaaa ttcatattct gcaggcgaga caataacatc agcaatatca
    83521 tcatgtctac cgagtccaga aagattcaaa atcggatcac ctagccattt ttgctctagt
    83581 gggctgacat aaactgggat ttgataatac gcgcgtaaag gttccactgc accgatatga
    83641 tcgtagtgag tatgggtaag caaaatcgct accggctgtt ggtttgtttt tttgatttct
    83701 tgtttgattc gttccgcatc ttctccaggg tcaatgatca aaagtgcttc ttcgttgtag
    83761 accaaataac agttttcttc aatcattcct gtttttaatc tttcgatatg catgtgcatt
    83821 cctccttgat agccaaaact tctttcatcc attagtataa cgcattcatt gctaggaaac
    83881 aaaaacaggt acttatttct ctttacttat ctgaattatc agactatatg aaaagtattt
    83941 ggatattcta aagagaagga aagtaagttt tcatagcatt ttaatatgtc cttgttacat
    84001 tagattcatc caaaaatcca aattctatat cctatatccc tgcctaatca gcagggtgtt
    84061 tttttatgtt tggaaagaga gctctttttg ctaaactagg ttttttcagg taatcttaga
    84121 gagaaagagg tggtggaaat gatcaaacag tcaaaaatat ctgtgcggca cttagtagaa
    84181 tttattttgc gacgtggaag tatcgacaat cgtaaaaaaa gtaaccatac tgcattagaa
    84241 ggagcgagaa tccaccgcaa gctccaaaaa gatgcgggag aaaactatga aaaagaagtc
    84301 tttttaaaaa caacagttca attagatgaa tattacctga tcgttgaagg ccgtgcagac
    84361 ggtattttca aaagagacgg tatttattat atagatgaaa tcaaaacctc cgaaccacga
    84421 tttgaagatt tagaaccaga acaagtggaa cttttctttc atcaagcaag ggtgtatgcc
    84481 tatatctata gtcaagaaca tgatttacaa gaaataaatc tgcaattgac ttacttccag
    84541 acggcagaag aagtgatcac gagaaaggtt gaacatcaga caagagagca gttagaaact
    84601 ttttttaaaa agctgactga agattataaa gaatggcttg tttttcaaga aaactggcgc
    84661 actgtacgca atgcctcttt gatggctttg aaattccccc atgatgagta taggaaaggt
    84721 cagcgtgaac tagcggtagc tgcttataaa acgatccgta cgaaacagaa attgtttgta
    84781 gaagcaccta ccggaacagg aaaaacaatc tctacgttat tccccgcttt gaaagctgta
    84841 ggggaagaag agggagaaaa gatcttttat ctaacggcta aaacaataac tagacaagta
    84901 gctgaagatg ctatgacagc attaaaagat acaggagctg aagtaaaaag tgtcactttg
    84961 acggcgaaag acaagatctg ctttttagat gagacgacct gtaatccaga tcagtgccca
    85021 tatgccaatg gttattacaa tcggattaat gaaggtttat gggatctttt gaatcatgag
    85081 aatcagatca ctcgtgaagt catcgaaact tatgcaagaa agcatacact ttgtcctttc
    85141 gaactatcat tagatgtcag tatctggtgt gacgtaatca ttggggatta caattattta
    85201 tttgatccaa ctgtttattt aagacgtttt tttgaagatg aaaaaaacga ggactatctg
    85261 tttttgattg acgaagcaca taatttggtg aatcgttccc gagagatgta ttcggcagaa
    85321 ctatcttatg aaaaaacgaa acgaagtaag gaagctatac caaaggaatt caaaaaactt
    85381 catcgccgtt tcaacaaact attaaaagaa tttgattcga tccgagaaat agcaaaagaa
    85441 gatcattggg attatcatca tcaaaaagca ccggctgaat ctttagtaaa ggcaggttat
    85501 caattaagcg aaaaaattaa agaatggctg gcagaatttc ctgagcaccc atcacaagaa
    85561 caattgcttc cttattattt tgatttgctt cattttttga aggtcagtga attttatgat
    85621 gatcactacg aaacaacagt cgaaaaaacg tatcgcgatc tgatagtaaa agagttttgt
    85681 attgatccaa gcttgtttct tgagcagtcg ttggataaag gaaaaagcag tattcttttt
    85741 tctgctagtt tttcaccact ctcttattat caggaaacac tggggggaca aaaaagtctg
    85801 gcttacaaac tgccgagtcc tttcccagaa ggaaaccagc aagttttcat cgcaaattac
    85861 ctagagacga cttatcgaaa aagggaacac agtctggcaa aattagtaga gacgattcat
    85921 gcttttgctg atggaaaggt cgggaattat ttcgttttct ttccttctta tcaatattta
    85981 gatcttgcgg tggaagcatt cagacagcaa tatcccgaca gcaatgtgct aatccaagaa
    86041 acggacatga atgaggaaga acgcgaacga tttttaacaa aatttcaaac aaatcccgct
    86101 gaaacattga ttggattttg cgtacttggc gggatttttt cagaaggaat cgatttgaga
    86161 ggggaccgat tgatcggcag tatgatcgta ggtgtaggtt tgccgcagat gaatcatgag
    86221 caggaactga tcaaatcata ttatgatgag aaggaacacc ttggatttgc ttacgcttat
    86281 cagcttcctg gaatgaacaa agtcttgcaa gctggtggtc gagtgattcg tggcatggcg
    86341 gatcaaggaa tcgttgtatt ggcagatcaa cgattttcta gtttttctta tcgacgttta
    86401 ttcccacaac attggcaaac agcaagagaa gtacgaagtg ttggggaatt ggaagaagga
    86461 atacagcgtt tttggttatc aaaagaagca gtaaatcaaa aaaacgagga atgacagacg
    86521 tcagcctcgt tttttgatta acgatagtta gtgaattgca actcgattgg taagtcaagt
    86581 ttacgtaata aagcaatcac agcttgtaga tcatcacggt tcttacctgt cactctaatc
    86641 tgatcttctt ggatttgtgc ttttactttc agtttttcat ttttgatagc tgtagtgatt
    86701 ttcttcgcgt tttcacgatc gattccgttg accaaatcgg cagtttgtcg agctttgcca
    86761 cctagagcgt gttctgattc ggaaaaatgg atatttttga ttggcacatt tcttttgatc
    86821 agcttaccaa ataatacatc tttgatctgt tcgattttga aatcatcatc gctcaataca
    86881 actagattgc tgttctctaa tttgatttct actgtagaat ccttgaagtc aaagcgattg
    86941 gacaattctt ttgttgtttg ctgaatcgca ttttttactt cttctgtatt gacttctgat
    87001 acaatgtcga agctagcatc ttttgccata ggaaccctcc tgatatacat ataaatattt
    87061 accatacatc tatcttatca taggattagg gcagattctt acctgtttgc taaaattctt
    87121 ctcatttgag ttcgaagtat tttattttaa tagataaact gttattctta catttggaat
    87181 accaaagtag gggagaggtg cttgtgagta aaattatgac tgcgtatcat aaaaatgaaa
    87241 gattaaagaa aatagtggtt attttgataa ccggattgac cgctgcggtc gctttaaatt
    87301 attttttgat tccagccaat gtcttttctg cagggatgaa tgggatcgct caaatcattt
    87361 cctcgctttt atatgaatgg tttcatatca agatagacac aggaacattt atttttcttt
    87421 tgaatatccc tgtgtttgta ttagggtttc tcaaagtagg aaaacgagca acgatcctta
    87481 gttttgtgaa tgttgtatgt gtatcggtga tgacgatgct gcttccagtc ggacagatca
    87541 ctgataatat tttgatgaat gccctagtag gcggtgtttt attaggtctt ggcgtaggat
    87601 tatctttaaa aatgggcttt acaactggtg ggatggatat catctctctt gtactttcac
    87661 aaacaactgg aaaaacagtc ggaaactaca tgctgattct aaatggtatc attgtagtca
    87721 tcgcaggctt cttctttaac tgggaaagtg ccttgtatac gattatttcc atttatgcaa
    87781 tgactcaagt aattgatgcc atccacacga gtcatcaaaa agtaacagca atgatcgtta
    87841 ccgtcaagcc agaagaggta acagaagaac tagcaaaacg gatggtacgt ggaatgacat
    87901 tactgccgtc tgtcggtggc tattcaaaaa aacaagggaa catgatcatg atggtcatca
    87961 cccgctacga aatgtatgat cttgatcaga tcgttcatga aatcgacgac gatgcattta
    88021 tcaatatttt accaactcat tcagtatttg gccggtttgc aaatgaaaac gaacaaaaaa
    88081 tgtatcgaac aactggcgta ttgccagaaa taaaaaatcg ttcacaaaaa aaatgaggaa
    88141 tcaggtttat tttagaataa atttaatcta ttacatctaa taagaacatt tttataaatt
    88201 taaacgagaa tcttacttct aaattctcat ttacaagcag ctaaaaaaag agtatgataa
    88261 ataaaaatcg tgttaaggag tgtcgtacga tgagcgaagc aaaccaagaa cggtctggac
    88321 aagaaataga actaatcaaa gaacgtattc ttgctgcctt ggaaatggtg attgatcctg
    88381 aattaggaat cgacatcgtc aatttaggct tgatttatga tatagaattc aatcctgaaa
    88441 atggagaaac agtcattaag atgactctaa caactatggg ctgtccatta gcagatattt
    88501 taacagaatc tatccatgac gcattaaaag aagtgccaga agtaacgaaa gcagaagtca
    88561 aactagtctg gtatccagca tggacgacag ataagatgag ccgttatgca cggattgcgt
    88621 tggggatacg atagaaatct tggctgacta gctatgagaa gcaaatagga aaaacaaaaa
    88681 agccttttat cctttctaga agaaaagaga taaaaggttt ctttgtttat aaaattttgt
    88741 ctgctatatt ataagttaat taaataaatg tgttctattg ataaaggaca ttcaaatccc
    88801 tttccttttg gaatccttct tttttacatc agtttgattt tcgtatagta ttctttctca
    88861 tcttcacgat aaatcgcagc cccatttttt tgttgtgctt taagactgcc aagatttttt
    88921 ttatttacag acaaaagtgc ctcatcaatg ccgatttttt ttgcctcttg tagtactaat
    88981 gctaatcctt gtgtggcata tcctttttta cgataattag gggagatgcc gtaaccaata
    89041 tggccagcgc cgtttcttaa tgtttcgttc agtttatggc gcaaattaaa aattcctaca
    89101 tactcatgtt catcactttc taaaataaag acggttgcgg gaacgtaact ctcaggaaga
    89161 tcgattccta aagacgtttt ttcgcatttt tttacataat ctaaaaattc agcaaatgta
    89221 tagccataag cattgttgat aaatccactc tcatttggtc caaagctagt ttgtagctga
    89281 taagctttct ttaaatctgc ttcccatagt ttgatcagtt tcatcgtatt cacctcttta
    89341 agtaacctta ttcatttgtt ttctactata gcactattgt tgtaaatagc catgcctatt
    89401 tttatttatt gtgtcatatc gtattaagaa tcatcgaaca atacaaatat gaattacaat
    89461 aaaaaaatta tttgaagttc gttcattcgt tacttatgat ctctagaaga aagaaaacgt
    89521 gaattacttc agcgtctaga attccagaag gatagtaatc aacagaagaa ataatcaata
    89581 tccaaatgaa agaagatagc tgaatcgaga aaaagaggaa aaaagaaatg aagaaaaaaa
    89641 taaaatagtt actaatccca gagtcgttcg tttaacattg aaatcgatca tatggataaa
    89701 acagattttt tcaccaaccc tcaatagatg atggagttgc tcggatgaat tatttgtgta
    89761 gatggcataa gggagggcgt acagaaatga aaaagaacaa aaatatgttc ttgacaaaaa
    89821 acacatgtaa tcctaaacta aaagatgtga tgcggctatg gcggaattgg cagacgcgct
    89881 agcttcaggc gctagtgagg gtgacctcgt ggaagttcga gtcttcttag ccgcataatt
    89941 tgttttttta taaagagaag gtacacctgg agcgatgaga aagaaaataa catattatct
    90001 aaaagaatat aggaaaaatt ttaaacaacg gaaccagatt caaataaatc aaaaaaaatt
    90061 tgtgtgaagg tgggacagtt taaaacctaa tagattgatt caaatagtag atttatagat
    90121 tctgatacaa gaaatactgg caaacgatag ttaaggaata aagactaaac ttaaactcaa
    90181 aaagaggtca tgcgaaagaa taaatcgcat gacctcttac tatattttga atgtgtttaa
    90241 acgttcaaa
//
LOCUS       DACECZ010000003        74668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.003, whole genome shotgun sequence.
ACCESSION   DACECZ010000003 DACECZ010000000
VERSION     DACECZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 74668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.003"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(200..598)
                     /locus_tag="IWO35_000230"
     CDS             complement(200..598)
                     /locus_tag="IWO35_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ0315316.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(595..1368)
                     /locus_tag="IWO35_000231"
     CDS             complement(595..1368)
                     /locus_tag="IWO35_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0315317.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1365..2048)
                     /locus_tag="IWO35_000232"
     CDS             complement(1365..2048)
                     /locus_tag="IWO35_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0315318.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2215..3102)
                     /locus_tag="IWO35_000233"
     CDS             complement(2215..3102)
                     /locus_tag="IWO35_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0315319.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3099..3968)
                     /locus_tag="IWO35_000234"
     CDS             complement(3099..3968)
                     /locus_tag="IWO35_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0315320.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4105..5391)
                     /locus_tag="IWO35_000235"
     CDS             complement(4105..5391)
                     /locus_tag="IWO35_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0315321.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5664..6242
                     /locus_tag="IWO35_000236"
     CDS             5664..6242
                     /locus_tag="IWO35_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0315322.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6242..7171
                     /locus_tag="IWO35_000237"
     CDS             6242..7171
                     /locus_tag="IWO35_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0315323.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7168..8904
                     /locus_tag="IWO35_000238"
     CDS             7168..8904
                     /locus_tag="IWO35_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0315324.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8967..9803)
                     /gene="pheA"
                     /locus_tag="IWO35_000239"
     CDS             complement(8967..9803)
                     /gene="pheA"
                     /locus_tag="IWO35_000239"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ0315325.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9800..10309)
                     /locus_tag="IWO35_000240"
     CDS             complement(9800..10309)
                     /locus_tag="IWO35_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ0315326.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10302..11597)
                     /gene="aroA"
                     /locus_tag="IWO35_000241"
     CDS             complement(10302..11597)
                     /gene="aroA"
                     /locus_tag="IWO35_000241"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ0315327.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11610..12701)
                     /locus_tag="IWO35_000242"
     CDS             complement(11610..12701)
                     /locus_tag="IWO35_000242"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ0315328.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12718..13884)
                     /gene="aroC"
                     /locus_tag="IWO35_000243"
     CDS             complement(12718..13884)
                     /gene="aroC"
                     /locus_tag="IWO35_000243"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ0315329.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13886..14950)
                     /gene="aroB"
                     /locus_tag="IWO35_000244"
     CDS             complement(13886..14950)
                     /gene="aroB"
                     /locus_tag="IWO35_000244"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ0315330.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14983..16005)
                     /gene="aroF"
                     /locus_tag="IWO35_000245"
     CDS             complement(14983..16005)
                     /gene="aroF"
                     /locus_tag="IWO35_000245"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ0315331.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16664..17638)
                     /locus_tag="IWO35_000246"
     CDS             complement(16664..17638)
                     /locus_tag="IWO35_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ0315332.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17619..19502)
                     /locus_tag="IWO35_000247"
     CDS             complement(17619..19502)
                     /locus_tag="IWO35_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ0315333.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19520..21745)
                     /locus_tag="IWO35_000248"
     CDS             complement(19520..21745)
                     /locus_tag="IWO35_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0315334.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21860..22894
                     /locus_tag="IWO35_000249"
     CDS             21860..22894
                     /locus_tag="IWO35_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0315335.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22961..23974)
                     /locus_tag="IWO35_000250"
     CDS             complement(22961..23974)
                     /locus_tag="IWO35_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0315336.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKHLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24052..24240)
                     /locus_tag="IWO35_000251"
     CDS             complement(24052..24240)
                     /locus_tag="IWO35_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ0315337.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(24680..24808)
                     /locus_tag="IWO35_000252"
     CDS             complement(24680..24808)
                     /locus_tag="IWO35_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0315338.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(24811..26184)
                     /gene="rlmD"
                     /locus_tag="IWO35_000253"
     CDS             complement(24811..26184)
                     /gene="rlmD"
                     /locus_tag="IWO35_000253"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0315339.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26295..27350)
                     /locus_tag="IWO35_000254"
     CDS             complement(26295..27350)
                     /locus_tag="IWO35_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0315340.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27360..28790)
                     /gene="gatB"
                     /locus_tag="IWO35_000255"
     CDS             complement(27360..28790)
                     /gene="gatB"
                     /locus_tag="IWO35_000255"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ0315341.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(28787..30259)
                     /gene="gatA"
                     /locus_tag="IWO35_000256"
     CDS             complement(28787..30259)
                     /gene="gatA"
                     /locus_tag="IWO35_000256"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ0315342.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30259..30564)
                     /gene="gatC"
                     /locus_tag="IWO35_000257"
     CDS             complement(30259..30564)
                     /gene="gatC"
                     /locus_tag="IWO35_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ0315343.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(30670..32706)
                     /gene="ligA"
                     /locus_tag="IWO35_000258"
     CDS             complement(30670..32706)
                     /gene="ligA"
                     /locus_tag="IWO35_000258"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ0315344.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(32723..34957)
                     /gene="pcrA"
                     /locus_tag="IWO35_000259"
     CDS             complement(32723..34957)
                     /gene="pcrA"
                     /locus_tag="IWO35_000259"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ0315345.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            35112..36671
                     /locus_tag="IWO35_000260"
     CDS             35112..36671
                     /locus_tag="IWO35_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ0315346.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(36782..38713)
                     /locus_tag="IWO35_000261"
     CDS             complement(36782..38713)
                     /locus_tag="IWO35_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0315347.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(38731..39645)
                     /gene="pfkB"
                     /locus_tag="IWO35_000262"
     CDS             complement(38731..39645)
                     /gene="pfkB"
                     /locus_tag="IWO35_000262"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ0315348.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(39642..40394)
                     /locus_tag="IWO35_000263"
     CDS             complement(39642..40394)
                     /locus_tag="IWO35_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0315349.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(40553..40762)
                     /locus_tag="IWO35_000264"
     CDS             complement(40553..40762)
                     /locus_tag="IWO35_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315350.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(40773..42101)
                     /locus_tag="IWO35_000265"
     CDS             complement(40773..42101)
                     /locus_tag="IWO35_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315351.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(42466..43053)
                     /locus_tag="IWO35_000266"
     CDS             complement(42466..43053)
                     /locus_tag="IWO35_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ0315352.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(43136..44386)
                     /gene="clpX"
                     /locus_tag="IWO35_000267"
     CDS             complement(43136..44386)
                     /gene="clpX"
                     /locus_tag="IWO35_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ0315353.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(44633..45922)
                     /locus_tag="IWO35_000268"
     CDS             complement(44633..45922)
                     /locus_tag="IWO35_000268"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ0315354.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(46076..47008)
                     /locus_tag="IWO35_000269"
     CDS             complement(46076..47008)
                     /locus_tag="IWO35_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315355.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(47165..47824)
                     /locus_tag="IWO35_000270"
     CDS             complement(47165..47824)
                     /locus_tag="IWO35_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ0315356.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(47827..48852)
                     /gene="sppA"
                     /locus_tag="IWO35_000271"
     CDS             complement(47827..48852)
                     /gene="sppA"
                     /locus_tag="IWO35_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ0315357.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(48865..49155)
                     /locus_tag="IWO35_000272"
     CDS             complement(48865..49155)
                     /locus_tag="IWO35_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315358.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            49565..51370
                     /gene="glmS"
                     /locus_tag="IWO35_000273"
     CDS             49565..51370
                     /gene="glmS"
                     /locus_tag="IWO35_000273"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ0315359.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(51442..52797)
                     /locus_tag="IWO35_000274"
     CDS             complement(51442..52797)
                     /locus_tag="IWO35_000274"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ0315360.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(52821..53990)
                     /locus_tag="IWO35_000275"
     CDS             complement(52821..53990)
                     /locus_tag="IWO35_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315361.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(53987..54874)
                     /locus_tag="IWO35_000276"
     CDS             complement(53987..54874)
                     /locus_tag="IWO35_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ0315362.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(55063..58743)
                     /gene="nifJ"
                     /locus_tag="IWO35_000277"
     CDS             complement(55063..58743)
                     /gene="nifJ"
                     /locus_tag="IWO35_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ0315363.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            58941..59462
                     /locus_tag="IWO35_000278"
     CDS             58941..59462
                     /locus_tag="IWO35_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0315364.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(60161..60726)
                     /locus_tag="IWO35_000279"
                     /pseudo
     CDS             complement(60161..60726)
                     /locus_tag="IWO35_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(60905..61561)
                     /locus_tag="IWO35_000280"
     CDS             complement(60905..61561)
                     /locus_tag="IWO35_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315365.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(62129..63064)
                     /gene="whiA"
                     /locus_tag="IWO35_000281"
     CDS             complement(62129..63064)
                     /gene="whiA"
                     /locus_tag="IWO35_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ0315366.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(63098..64096)
                     /locus_tag="IWO35_000282"
     CDS             complement(63098..64096)
                     /locus_tag="IWO35_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ0315367.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(64093..64977)
                     /gene="rapZ"
                     /locus_tag="IWO35_000283"
     CDS             complement(64093..64977)
                     /gene="rapZ"
                     /locus_tag="IWO35_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ0315368.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(65112..65843)
                     /locus_tag="IWO35_000284"
     CDS             complement(65112..65843)
                     /locus_tag="IWO35_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ0315369.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(65847..67112)
                     /locus_tag="IWO35_000285"
     CDS             complement(65847..67112)
                     /locus_tag="IWO35_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ0315370.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(67375..70191)
                     /gene="uvrA"
                     /locus_tag="IWO35_000286"
     CDS             complement(67375..70191)
                     /gene="uvrA"
                     /locus_tag="IWO35_000286"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ0315371.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(70201..72195)
                     /gene="uvrB"
                     /locus_tag="IWO35_000287"
     CDS             complement(70201..72195)
                     /gene="uvrB"
                     /locus_tag="IWO35_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ0315372.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            72454..73956
                     /locus_tag="IWO35_000288"
     CDS             72454..73956
                     /locus_tag="IWO35_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0315373.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            73958..>74668
                     /locus_tag="IWO35_000289"
     CDS             73958..>74668
                     /locus_tag="IWO35_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0315374.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKD"
BASE COUNT        20886 a        16213 c        13210 g        24359 t
ORIGIN      
        1 aactaaaaaa ttcatttttc tccccctttt ttcatcattt ttaaaatagc atattttgaa
       61 aagaaagtct gatgcttaga gtagaatttc ataaaaattt actatttcat aagtattcac
      121 gagaaaaata caaacaaaaa agcggaaata acgctcatcc gccttcttgt ttgtgtttat
      181 ttcctcttca aacgcttgtc tatgattcag acttttctct atcgttattt tcatctaaaa
      241 gttcttgcat tccgataatt tctcgatatt gatgaatctt agtgatacca atgactttgt
      301 ctacatcaat actaaaacaa ataccattac ctggaatatc gatgttgatg ccttgaccaa
      361 tcgcatccat tactttattc gtcaaactat cgggtactaa tgtcaacaca atatcttttt
      421 ctggcgaaat gtccaaacct aaaaatttcc ctgaatcatg tacaccagaa cctcttccat
      481 gtaagatcgt tccgcctgct gcacctgctt cttttgaaaa gtcgataaca tcttcgccca
      541 ttcctccgtc aacgatcgtc acaatcatgt gaagattcaa tgctacaaga tctttcattt
      601 gctttccccc aatttttgca atctttcttt ttcgattctg tctttttcat cttttcgttt
      661 ataaatatat cctagtacta aagtggataa tattggtgtc aatgcaatca ttgcaaccac
      721 accaaacccg tcaatgatcg gatctcttcc ttcaatttgt gcagcgattg ccacgctcat
      781 tgataaaata aatgtcgaac acatcgggcc cgttgctact ccaccgttat caaaagccat
      841 tgctaaaaag atcttgtcca cttttcgtcc taaaataaat gctagcccgt atcctgggat
      901 caagaaataa taaagagaga atccgacgag caaacgccac atagacaatc ctactgctgc
      961 gccgatccca attgaaatta ccatcagcat gattttcgat cggattctcc cctcgctcaa
     1021 ttcttcaacc tgtttgatca ttacatgaat cgccggctca gcaaacccta ctaagaaccc
     1081 cattaaaaag ccaaggggaa ccagtatata attgtaaggc aatcccgcaa tagcactgcc
     1141 taaatgctgt cctaccggta cataagccat attgactccg tgcaggaaaa ggatcaaccc
     1201 aatagtcgtt atcaagaaac ctttcataat gtcaaagaaa cgctttttgc gcatacgaaa
     1261 actaatgata ttcattaaaa caaataggat tacgataggt gccactgcca aaatgacttc
     1321 catggtaacg atttcgattc catctatcac ttgctgccag aaactcattg gaagatcacc
     1381 cccaaaatca tcacggcaag aatcggccct aacgaagcaa taccgaccat tccaaaactg
     1441 tcattttcat ttttcttttg tttgatcata ctagtcatcc ctccggcaag ggccaagata
     1501 aatggaacag ttactggacc agtagtcact ccccccgaat caaaagcaat cggcataaat
     1561 tcggaactgg tgaaaaatga ggcgatcaaa acgcctatgt agcctatagc catcaattga
     1621 taataggata gcttgaatac gacccgaagc agcgcaaagg ctagaaatac tccagtgccg
     1681 acgctgacga tcccaatcag caacactctg ccaatctctc cgtcagatat atcatatact
     1741 tgttgtccca aaacttgcac ggaaggttca gcaatcgtga tcacgatccc gatagcaaat
     1801 cccaaactga ccaaaacggt caaacttttc tttttgatca tatatttccc tactagatct
     1861 ccgacttcca tcatagaata atctgctcca aacaaaaaga gcgtcattcc gatcatcatg
     1921 attgccgcac caaccagaaa tgaagcgagg tcttctcctt ctaacggtgc aaaaacaaac
     1981 gttaaaatga caatcaggac agtcatcggc aatatagcag caatcacttc tttaaaattt
     2041 tttagcatgt atcctcctcc tttactcttt aagtttacct taaaatgaaa gagttttcga
     2101 gcacaaactt aaaacgttat catttaattt ttctcaacat aaaataacac acgtcccaaa
     2161 agtatctttt tacttggaaa aatgactttg aaggaacgcg cttattgtgt ttttttattt
     2221 tacagagccg atcattcctt gaacgaattg cttttgcaaa acaaaaaata cgatggcagt
     2281 tggtaaagta gcgatgacta atccagccat gatcattcca tagtctggtg tataggaagc
     2341 acccatagca gataaaacaa gcggaaccgt ccgtttatca ggagattgca aagctactaa
     2401 gggccataaa taattgttcc agctactcat gaatgtgatg attgcagccg cggcataggt
     2461 atttttagcc gtcggcatat acactctaaa aaaaatactt agttctttta gaccgtctaa
     2521 tcgagcggct tctactagat cttttggaaa agatttaaca ttttgtcgaa agaagaagat
     2581 caaaaaagca gtgctaacag atggcaggat gacaaccaag aaactgttga ttcccaaaac
     2641 cgttccgttc aattgtgaaa acattcgata taaagggatc atcaatgccg caaaaggaac
     2701 catcatcgat agcagtagaa tattgaaaat taggtctttt cgtttacttt gataaatctc
     2761 aaatccatat cctgccattg acgaaatcag caatgcaaac acagtggtca ccaaagcgat
     2821 catcgctgaa ttccaaaggg aacgagtaaa attcagctca ttggaaaaca aattttgaaa
     2881 attgatgatc aagttactgc caatctttat ttttcccgat gtgatatcca cgggtgtatt
     2941 cgtcattccc aaaaccatcc agacaaaagg gaaaatggaa ataaatgata ggatgcttaa
     3001 tgctgtatac tttaatactg acccaattgt cgaacgaagt tctaatgctt gttttttgac
     3061 gttggggcgt tcgattgttt ctttttctac catatttttc atcgactttc tcctcctact
     3121 ttcgtttgga ttatcgacaa tatcacaata aaggaaacaa taacaaagga aactgctgcc
     3181 gcatatccaa aattaggcgt gaatttataa ctcaaattat agatgtattg agatagggtg
     3241 gtcgttgcat ttgctggtcc cccgccagta atattttgta cttcatcaaa aagctgcaat
     3301 gtcccgatcg tcgaagtaat ggaagtaaac aaaatgattg gttttaaatt tgggatcgtc
     3361 actttgataa actgctgcca tttagaagca ccgtcaatag atgctgcttc ataaatctcc
     3421 gaatcaatat tttgcatgcc tgaaaggaaa aaaatcatat tgtatccagt ccaacgccaa
     3481 gtgatcgaaa gaatgatcag aactctcgcc caaattggat gagtcaacca gccaatcggt
     3541 gtgtcaatca taccaatgcc caacaacaag ttgtttacaa ggccagattg agaaaataga
     3601 cttttcatga tcagagaata actgaccatc gaagtgatac atggaagaaa gatagcggtt
     3661 cgaaacaacc ctttatattt caacttttta tcatttaaaa ggttggaaat aaaaagtgct
     3721 agaaacaaca tgatcggtac ttgtacaagt aaatacagga atgtattaaa aaaagctttt
     3781 ttaaaagttg catctgtcaa catccgctga taattaccta acccgttaaa tgtcatattg
     3841 acagggggac cactttgaaa agacgtgatc aaagccaaaa ccatcggaat aaaaaccatg
     3901 attgatatca aaatgactgg caagattaaa aaagcatgcc cgtttttgaa taaatctttc
     3961 tgcttcattc ttttccctcc aattctatca ttgattatct acatgaaaag acaagaaaga
     4021 caatatctgt aaggcagacg tgatcttttt tgtcttttcg tttgtctgat gaaaaagcga
     4081 agaaattcac ttttttacca ccgatcaatt agccaattgt gcttctactt gtttttgtgt
     4141 atctgctagt accttgtctg tctcttctcc attgatgatt cgatgaagag cttcagctac
     4201 gactgcttca attgcatagg tctcatgtcc gtaattcact tctggtattt cagatgtcca
     4261 tgttgaaaaa tcgtcaaaga tcttttgacc actgtaaaat tcagaagctt cttgataagc
     4321 ttcttgttca ttcgcgctaa gcatcgtaga tactagtccg atttctttcg caagagtgcc
     4381 catcagttct tcattcgaag caaacgttgc ttctaggaaa tctttcgctg aatcttctcc
     4441 agctactcct ttgatcacat accagccagc gccgccaagg ttagaagctt gcgctttttg
     4501 cagattttct ggaagtgcag gtattggtgc aatcttccat tttccagatt ggttttctgc
     4561 tccttggatc gttgaagaat accaagcgcc agttgggcta gaagctactg ctcctgattg
     4621 tatcgcagtt actccagcat tccagtcaga ggtttgttcg actagatctt ctttcaaaag
     4681 agtggcaaac aattcaagac catactttaa agatgcattg tcttggatcg tgactgtctc
     4741 cccatcttca gatgtatacc attctcctgc ctgttgcata atcatcctta ctcgaccaag
     4801 gtcagaagga ttgacttcag cgattttctt tcccgtcttt tctttgacat ctcgcgctac
     4861 ttgaatatac tcgtcccact tcagattctc catatcctct tctgtataac ctgcttctgc
     4921 catcagatcc gtacgataga agtttgctgt tacgcccgaa tcgaaaggaa caccgtatat
     4981 tttatctcct actttgttta ccgcaaattt atacggtgca aaatgatctt cctttacaat
     5041 agcagacaaa ttagcgaatg catctggata agaagtcaaa tagccttgta tcctataatc
     5101 ttcaatcaag acgatattgg gcaatccttc tgtattcccg ctcgctaaag cggtgttcag
     5161 tttctgaacg atatcatcct gagacatcgt aacaacttct atctctgttt cttcgttctc
     5221 ataaactttt tttgcttcat tcactgcttt gatattgaat gtctcatccc acgcccagac
     5281 agtgactttt tcttctttgt ctgctgtact actagcactt ccgcatcctg ttaagaaacc
     5341 tgatgctgcc gctaaacaaa cgactccagc aattaacttt ctgatgttca tttttatccg
     5401 ctccttttcc atccattagt tgtttacgtt tgctacattc atcataccaa aatttgaaaa
     5461 cggtttcttt attattcaga tacttcaaaa atttctccta aaataataaa aaaatacaaa
     5521 tcaactatga caaaaggaat aatcttagca tctagaccaa agatcgaaat ggtgaggttt
     5581 ctttaaaata aaaatatcct tgcattttct cctagaaata agtattttta aattacaaga
     5641 ctattcttaa ggcgggtaaa aaaatgccaa tacgcgtaat tcttatcgat gatgaacaga
     5701 cgattttaga tggaatgaaa cagttgatca attggtctga gcacgggttt caattaatag
     5761 ggactttttc cagtccgctg aaagcgcttg atttttcttt ggtacatgca atcgatatcg
     5821 tggtcacaga tgtaatgatg cctgaattaa atggaatcga attatcgcgg ctattaaaac
     5881 agcaacaacc tgaaacacag atccttatcc tctctagcta tgatgaattt gatatggtta
     5941 aagattcttt taaagaaggc gtcgctgatt atcttttaaa gcctcgattg agtcctgatt
     6001 tttttctaaa ttccttaact gccgtttcac aaaaaatcaa aaaaaagccc aacaaaagcc
     6061 caatcaccaa tcgttatgag cagatctgcg agaatctttc acagcagatt gctggacaaa
     6121 ctcgtccatt gcttgaccct cttctttttg aacacacctt gtttttctgt tatataccga
     6181 aaaacggcca ttcagttcaa aacaaggctc taaacaaaaa cagtcgatca cccattcatt
     6241 gatggatagc gacgattcgt ttactattta tcctgtttct accttttcgg aagaatccgg
     6301 ctatgtaatc aatactgatt ccaaagatac acttaggaag tttgttcaaa agtttcgtca
     6361 agaagttttt ccaggtgatt tttttgttgt ttctgattct tttgcaatcc aagatatcaa
     6421 tgaaatattc caagagatca gaaattgcac caaaggacaa cgtttctatc ataagggaaa
     6481 gaaccttgct tatcagcatg aacttcttcc gttattaacc aaccacgagc agcaagcaag
     6541 tagttattta acaaaaatcg ttcataaaga ctacgcttta gctgtcaaag aacttgagaa
     6601 tttcttcacg acgatattgg aacagcctat tgatccagct tatttgaaac atgaggcaat
     6661 caatcgtttc tatgcattac tcaacgcttt ggctgacgag catgttggga atgaagaact
     6721 ttctttgctc aagatcgccc ttccttcttt attagccgat gctgagtatt tcgaagattt
     6781 ttctattctt gtcaaagaaa cacttgagaa actcacccac ttttctgcaa atgcaaaaaa
     6841 agacaatttc gatatcttga ctttgatcta cgagttcgtc caggaaaatt atgagaaaga
     6901 aatcaattta caacttttat ccgaaaaata tcatttttca tatagctatc tctcagccat
     6961 ctttactgaa aaatttggta tcaacttttc aaaatatcta aaaaaagtcc gtatcagtaa
     7021 agccaagctc tttttgacgg aaacacggct tactttgacg gaaatctgtg agaaagtcgg
     7081 ctataccgaa ctgggttatt tctccagagt ttttaaagaa gagacaggaa tgacaccttc
     7141 tcaataccgt aaaggaactt ttgtaaaatg agaaaaaaaa tcaaagagca tgaactcttt
     7201 gccaaacttc tgttcatttt gttcatcggt ctttttctgc aagccatcgt catttctttg
     7261 tttatttatc atcgatcaag ggatgcctat atccagctgt tcaatcaatc gaatgatgtg
     7321 gtactaaaaa agattcaatc tgaatttgaa agtttgaatg atacaatcga gaatacatta
     7381 gccgcttttg attctaatcc agctgtcaaa agctattttt ccaatgatcc agcccaacat
     7441 atggaacagc ttcagcaact aagaacaatc caaaaaatga atgactctct ttcaaaaatc
     7501 catccaatga tcgattacga tgtattgatc tttggagaaa atggaagaac ttttgtcgga
     7561 aacgacatgc tcactgccgt atctgccgat tctttttttc aatctgcgat tgctcagaga
     7621 gtgaatgaac gtgctgctga tacacaaatg ctttttgcac accacggatt gactcttcgc
     7681 gataagaaag cacccagcgt cttctttgtc aaaaaactga aaaatacatt gaatcacgtt
     7741 tacggatatg ctgttttatc gatctcatcc aagcaactag cgaatctttt tcaatctgta
     7801 gtaaatcctg agatatcgaa gatctccttc atgaacgaag aacagacgat catcgcatca
     7861 aatgaagaaa atacaattgg gagccgatca atgattttca aaaaactaac agtaggtgaa
     7921 acaacgattt caaatggaca gcagttgacc cgtcttcctc tgtaccgcca ggattgtaca
     7981 ttactcagtc aagtcgatct tcattcttta tctaaccaaa tgggcgtgat ttttccgatt
     8041 attttgtata atatcgtgac acttgctttt gctggctcat tggtatttat gtatttggat
     8101 cgacacacaa aatcgatcta ccgcttgatt gattcgctaa aacggctaga ggaagatggc
     8161 ttaaaagcta ctgttcctgt acaaggaagc catgaagtcc aattgctagg gaatacgatc
     8221 aatcaattgc taagtaatgc tcagcatcat tatgaacaga cgatccaaga cgaaaagaag
     8281 aaacgttcgc ttgaaatcca aactatgcaa gcacagatac agccccattt tatttataat
     8341 acgctgacta gcttgaaatt tttgatttgg cagaaaaaaa cagatgaagc agttcaagga
     8401 atcgacagtt tcattacttt actaagaagt acgattggga aaaaagaaga agtgattcca
     8461 gtcaaggaag aaatcaaaag cgtacaaagt tacatcgatc ttttgagtat gcgatatggc
     8521 gaaagaatct cggctaaaat aatgattcct gatgaactgc agtcattatc tatgccgaat
     8581 atgatccttc aaccaattat cgaaaacgcc tatcttcatg cttttcacaa taaaacgcaa
     8641 ggattcatca cggtgtacgg caaaacaaaa aatcagcttc ttttattcga agtgattgat
     8701 aacggcgatg gcttcgatac tgaaaatacc aaacaaaaaa atgatcattt ttcaggaatc
     8761 ggcatcagcc atgtagatga gcggatacgc ctaatgtatg gcgatgcata tggcttaacg
     8821 atccaatcag tgattgggac aggaacgact gtaacgattt gtctaccgct tcttcctaaa
     8881 aatcaatcag aatatcaaaa ataaagaata gccaagcgct gattttttta ttttaagtta
     8941 aataaatcaa tcagtattct tgactttcat ctacgcgtct cttctactgt cagcactgga
     9001 taacagccca atatttgact ttctcctccg agcatcttga tttcttggag cgcattctct
     9061 actaatgtca ttggacggtc caacaacaaa tcgatcacga aaaaatattc tcccaagcta
     9121 gttttcaatg gtcgggattc aatcttactt aagttgattt cccgccagcc aaaagctgcc
     9181 agcattttat gaagcatccc tggccgatta gcaggtagtg tcaagatgac actcattttt
     9241 actggagctg actgctggct ggtcatcttt cggtcaccga cgatccaaaa acgtgtctgg
     9301 ttcaactcat tatcttggat attctcagct aagatctcca atccaacatg ctgtgctgtt
     9361 tctaatgaag caatcgctgc tgcgtcttct ttcggatgtt ctgctacata cattgctgca
     9421 gcagttgttg attctgttgc tactaaagga acattaggat aatgcgtttc taaaaactgt
     9481 tgtgattgtg ctagtgcttg tggatgtgaa aggatttttg taatctttgt tgctggattg
     9541 cctaaaagct gctgcttgat aggaagaacg atttcacttt taacttcgac ttctggatgt
     9601 ttggaaagaa gatcgattgt gtgatgaaca gctccttcca atgaattctc tacaggaacg
     9661 acagccagat cgacttgttt gcgaaacaaa gcgcgcaaac aaacagggat ggaagcatac
     9721 gcagtcaatt gttctgtagg aaatagctga caagccgcct gataagtgaa tgaactttct
     9781 ggccctagat agctgacgtt catatgcttt tgatcctttc tataatggta tcgatgatct
     9841 tttctggtgt ttgattggtc gtgttgattc gatgtgtcgc actttcttca taccaagaaa
     9901 gtcgttgtgc caataatgct tttatttctt ctcttgtttt tccaagagct aaaggtcgaa
     9961 tagacgtttc atcttgttgg atacgatcaa ctagacagtc cgcttctgcc tctaagtaaa
    10021 gaacaatcgg ctgtgttttt aaaaaacgcc gattttctgc actttggaca atccctccac
    10081 cagtcgcgat aattttgtct tcatttgaca gctttcttaa aatatctgac tccacttttc
    10141 gaaagctttt ttcaccgtaa tagtgaaaat attcggcaat cgacaattgg atatgttcct
    10201 caatctttgt atctaaatct acatactctt tttgtaaaca ttgagctaac cctttaccga
    10261 ttgttgtttt tccagcgccc atgaaaccaa tcagcaaaat gctagccatg agcttctcct
    10321 cctgacacaa gttctgctaa atcgtcaaaa aatgccggat aagagattga aacagcttca
    10381 gctccttcta gttcgcaagg tccatcagct atcaaagcag ctacttgcag catcataccg
    10441 attcgatgat cgcctctgct ggacactttt gcgccatgca gaggtgtcgg cccatggatg
    10501 atcagaccgt ctgctgtcgc ttttatcttt gctcccattt tctgtaattc ttctgccacc
    10561 gcatcaattc tgttggtttc ttttactttt aattcttctg cgtctttgat gatcgtgatt
    10621 ccttctgctt gagtagccac taaagctaga atgggcaatt catcaatcag acgcggaatg
    10681 atttctccac caatcgttgc tttcttcaaa tgactcgtct tgacacttaa atcagccgat
    10741 tggttctgtt cattgtgatt cgtctgggta atcgctgcgc ccatctcctc caacacatct
    10801 aagataccag ttcttgttgg attgaccccg acatttttta acaacagttg gctttccgga
    10861 acaagaagtc ctgctgctag aaagaaagca gctgatgaaa tatcacctgg aacagttatc
    10921 tgttgaccag ttaatttttg cggtccggtc actatgatcg ttttatcttc cactgtgatc
    10981 cttccgccaa attgacgaat catttcttcc gtatggttac gggaacgttc tttttcaacg
    11041 attacggatg tgccttctgc ttgtaatgcc gcaaacagca aagcagattt gacttgcgca
    11101 ctggctacag gcatattgta tcggataggt gataaagagc gggttcctgt cacagtgatt
    11161 ggtagatact ggtcattttc ttctccttgc aagtcagcgc ccatctcgcg taaaggttcc
    11221 atcactcgcc ccattggacg tttagacaac gaagcatccc cgaacagagt agtcgtaaaa
    11281 ggctgtccag ccaaaatacc catcaataaa cgtatggtcg taccggagtt tcccatgtca
    11341 attgattcgg ttggttggac aaatgagtgg ctaccgcgac catgaatctt gatcacctct
    11401 tcctcttctt cgatctcgac acctaattgt tggaaggcat gtaaagtact cagacaatct
    11461 tctgcgcgca aaaaattttg tatagtcgtc gttccttcag caattgctcc aaacatgatg
    11521 ctgcgatggg aaattgattt atctgctggt atcctaacag tccctcttaa tccatgtatt
    11581 tgttgtaata attgcattta tccagcctcc tatttgatcc agcaatgata actggtatta
    11641 gacataatga ttctttttgc ttgtaacaaa tcctgttggt ttttaaaact gatttgcagt
    11701 attcctaaaa tatcttccct ggtctcttgg atcttcaaat taatcaaaga aactcccgct
    11761 tttccgatca gtccagttac ttctgcaata acgccaggtt catctggcac atcgataaat
    11821 aaatcaaaaa aggcaggaat cgcgccttgc ttatggacag gcaactggtc tcgactttct
    11881 tttgcccgat tgaaaaaaga aaagatttcc gattggttct cagtcatgat ccattctgct
    11941 atttgtgtca tttcttgttg ccaatctgcc aatcgttcca ataatatctg cttattgctc
    12001 aacaaaatat ctgtccacat gacaggatct gaggaagcga tcctagtgat atctcgaaag
    12061 cctcctgctg ccagttgttt ggctcgcgga tactcttgat tgaattgttc tgcctgattg
    12121 actaagccag ctgccagaat atgtggaaaa tgacttagca ttcctgttat acggtcatgt
    12181 tctcttgctg taagagttac atattttgct ttggttccgc aaaatagttc ttgcagtttt
    12241 tcaacatctt ttttcgtctt ttccaaaggt gtaaagatgt aataggcatt ttcaaatagt
    12301 ttagcatctg ctgcctgtat acctgattta tgagaaccag ccattggatg tccaccgaca
    12361 aaccgaagat ttttctgctc agcggatgtc acgacttgct gcttcgtact gctgacatcc
    12421 gtaatgatga cgtgatcaga caagttgatt ctttcaagga catcaagata agctaaggtt
    12481 gtacggacgg gaacggctaa taagataacg tctgcttgtt ctgcacctgt ttcaaaatca
    12541 cttgcaacgt aatcaacgat ctgctgttct actgcttgct cacgggtttt tgactgacta
    12601 tcccaaccaa ctactatcag ttctggattg gtctgacgaa tacaaagtgc catagaagca
    12661 ccaatcagtc caagccccac gataaaaaca tgttgtgcca tcttatttgc ccccttttta
    12721 gtaatgtttc aagtagtcac ggtactcctt cactgcagct ttcaactcaa caagcgaatc
    12781 acttgggaat tttgacaata gctctgtcgc taccacagtg gctacaacat tttccgcaac
    12841 gacgctagct gcaggaactg ctgtactatc cgaacgttct acacttgctt tgtaaggttc
    12901 ttttgtatcg atatcaacac tttgaagtgg cttgtacaat gtaggaatgg gtttcatgac
    12961 cccgcgaatg acaagtggtt cgccattggt cattccgcct tcgaagccgc ctagattatt
    13021 ggtacgtcgg aaatagcctc tttcttcttc ccataggatc tcatccatca cttctgagcc
    13081 tggtaatgaa ccagaagtga atccgacccc aaattctacg cctttaaagg cattgatact
    13141 aaccacagct tgtgcgattt tggcatctag cttcgtgtcc cattgtacgt agcttcctaa
    13201 tccagctgga acgcctccaa cgagcacttc tacaattcct ccgatcgtat ctccattttt
    13261 ctttgtctga tcgatcaaat cttttattct ttgctcaact gctggatcaa caactcgtac
    13321 ttctgacgct tcagaaattt ctttgatttc ttgaatcgag atatttccag gtatttctgc
    13381 ttggattcca ccaagacaaa tcacgtgtcc tgctacgatg atgtcaagtt cttccagaat
    13441 tttcttggca attgcgccga ttgctacacg catggtcgtc tctcttgcag aggaccgttc
    13501 aagcacattc cgaagatctc gatgatggta tttcataccg cctaccaaat ccgcgtgtcc
    13561 tggacgcgga cgtgcaactc gccgcagtga ttggtctttt tcaggcactt ctgctacaga
    13621 catgacagtt gtccaatttt tccaatcttt attttctacc actaaagtga caggtgaacc
    13681 aattgttttg ccgtgacgga tgccagacgt gatccgaaca cgatcttttt cgatcttcat
    13741 ccttcctcct ctgccgtaac cagcttgtct gcgagcgagt tcgtgattga tttcttcttc
    13801 tgacagaggt aatcctgctg gtagtccttc aataatggct gttaattctg gtccatggga
    13861 ttctcccgca gtcatataac gcatcttaca gtcctcctaa gtaatctttg aatgtctctg
    13921 tgggaacagg aacgattttt gcttgcccaa tcttttctag caagatgata ttcaattggc
    13981 ttcctcttgc tttcttatca tgggttatcg cgtggaataa ttcatctgga tgacttagat
    14041 cgtacgtaat cggcagatgg aatttttgaa gtatcctttc taaagcagct gtcgatccag
    14101 ttggagacaa gccgattttt tctgcatgag aagtgatggc taccattcca atagctactc
    14161 cttctccatg actgactacc ccatatcctg ctgtattttc aattgcatgg ccaattgtat
    14221 gaccaaaatt taaaataagt cgatttcctt ggtcaaagac atcttcttcc actacttttc
    14281 gtttgatttc caacgtagca gtaatgattg gaacagcatg ttccagaaga tctttttcgt
    14341 ctttcagctc gttcagctgt ttccaaagcg ttgcatctgc tatagctgct gatttgatga
    14401 tttcagcgat tccttctcgt aaacgacgta ccggcaaagt ttttagaacc tcaggatcaa
    14461 tcaatacacc agctggctgg ctaaatgttc cgacaaggtt tttcgctttg tttgtattga
    14521 ctgctgtctt cccgccaata ctagaatcta cttgtgcaag tagcgtagtc ggaatttgaa
    14581 cgaaatggat cccacgcatg aacgtcgagg ccacgaatgc tgctagatca cccaccattc
    14641 ctccaccaag tgcaagtatc ccatcgctgc gggtaaaatc attatctgct aaaaaatcgt
    14701 ataactcagc tgcttgttct aaagatttac tggtctcacc cgccggtacg attgaaagga
    14761 ctggttcaaa attagcttgt ctcaattgga acatgatttt ttctgcatat agtggagcaa
    14821 cgtttgtatc agtgatcaca gctacttttt gaggtctcca aatcgtttga agccatgatc
    14881 cagcctgttc tagtgcgcct ttttcaatat gaatatcgta tttttggtta tcaacatgta
    14941 cagtaagcat tcgtttcact tctttctttt aaaaagctgg aattaatcga gtgtctgaat
    15001 cttttccatt gcttctttca tgatcgcaac ttctttcatc atgcgtgagt aagtcttttc
    15061 attcaacgat tggggaccgt ctgaccaagc atttgcagga tcaggatgga tctctacgat
    15121 catgccatct gctccgcttg ccacacccgc acgagccatt ggtggtacaa gttcccaaat
    15181 tcctgtacca tgacttggat caacaatcac agggaaatga gttaattttt tcatcaatgg
    15241 gacagcactt aaatcaaacg tattacgtgt cgctgtttcg taagtccgaa tcccacgttc
    15301 gataaaaatg acaggagatt tgtcttcgac tgcaatgtat tctgcagcat taagccattc
    15361 attgatcgta ccggaaattc cacgtttcaa tccaacaggt ttacctgttt taccgacagc
    15421 agatagcagt ttaaaatttt gcatattacg tgcgccaatc tgtaggatat cgctgtactc
    15481 agccaccata tcgatatgcc cttcatccat cacttcagta atcactttca ttccaaattc
    15541 atcagccgct tgacggatgt atttcaatcc ttcttcttct aaaccttgga aagcatatgg
    15601 agaagtacgt ggtttgaagg caccacctcg cagaattttt gcgcccccgg ctttggccat
    15661 tcgcgcacat tctctgattt ggtctaatcc ttcgatagaa caaggaccag ccatcgtcac
    15721 gaaactgccg tcaccaattt ttactccgtc tacatccacg actgtattgc caggatggaa
    15781 ttctcgagaa gtcaatttgt atgtgtttaa aatacgcaca gcattttcta ctccttcata
    15841 gcgtgtaaat gctccttctt gtaaacttcg cgtatcacct tttagtccaa tcactactcg
    15901 ttctgttcct tgatcgattg agactttgaa tccttctttt tcgatatgat cgacgacttt
    15961 ttttatttgt tcttccgttg cttctttttt cataattact atcattgttt ttcccgcttt
    16021 cttttgttta cttatctatc ttggctgatt ttctctactt tttcaaggtg gttttacatt
    16081 aattcctact aaatgaccgt gtcctgttgt tctcattttc gctccttctt tcctacttat
    16141 gacccagata atcaacaaaa aaagatgtcc ctgtaggaaa ttgcctctac agtgaacatc
    16201 ttcaacgagc cttaaaaagc gctgcaaaaa tataaaatag ccactataga tgaatttctc
    16261 tacgtggctg taagtcgtgt ttaaaaatct ttagccatca tagatacaaa cctggtttgt
    16321 ttgtacccac gaagtcatga atacaaacct atctaaaata gctaaagtaa tacgtattca
    16381 gttttgtaag ttttctagat ttcataaaaa atcgctccaa actgatgtaa gaattgtacc
    16441 tagtatagac ataaaaaaat cgactgtcaa tcatttttat gaaacttttt ttaattttct
    16501 gatagttaac tttgtttttc agaaaaataa caattggttt tattgaagaa aatagtcatg
    16561 aacaaaagta tctgagaact tttttactct cttgttcatg acatatagaa taaagcagct
    16621 ttgtcttgta aactccgttt ttcaaataat aatctaaaga ttgctactga ataataaagc
    16681 cgatttcatg gtcctcttcg ctgctgattc ggtaaggtga ctcaacatcc gctccccagc
    16741 tattgatacc cccgactcct cttacttttg caaaaatcgt aagaaccgtt cgtctttctg
    16801 gcggtagttc ttctatatgt gttgcctgtt ccagttcttc tgctttgaat ggtaaagcgg
    16861 aaaaagcaaa tggcgtatcc tgctgttcaa atcgcaacga aaaatcttct tgatctttat
    16921 ccgtattatc caacgtttga tttcttgtta gttctaccca tttcgtttcc atatgcatgc
    16981 cgcattcttg gggaacaagg tatggggaaa cggggagtcc tgccacttta taaatacctt
    17041 cgacaccacc agccatccga tccggatatg tttcccctga taggccttca tactcaaaaa
    17101 attttgcttt cgttggcata atgaacctta aacccaatgc gggtaattcc ggtaatcctt
    17161 tttgtccata ataatgcata ttgacaagca ttttaccctc tccagcgatt tggtaggaaa
    17221 ttgtcacttg ggtttttggt actgtgttga tttccagtgt aaaaatgatt tcaaccgttt
    17281 cagcgtattc attacttgaa tattgattat ttactggagc aatcggcaaa tcgatctttt
    17341 tattgtccac cagtacttct atttctttga cttttggata caatccggct cctaaccaca
    17401 taccggattt aaatccaaat ccgctacctc tgtcattatc ggtcgttgct cgccaaaaaa
    17461 ctggaagtgt ttcccggtat agccattctt ttccgttttt attgagcgat tccagtccgc
    17521 ctcttgtata agaaaaaata taatgcgtat tccccgattt gacacctaat gtacagtcgc
    17581 cgaagacgat tgcaactttt tccgtatcca tatgactttt atttactgta tttttcataa
    17641 taatatctta cctcctgcat ggcgggtttt tcttgtccat ctgcaaaaac gatcccattc
    17701 cctgagaatt catagtcact tggtttatcg tcaaagtcac cgccatagcg catcacttct
    17761 ctgcctgtga ttggatcagt cacttggatc gcctgatctt taaaatccca tatatagcct
    17821 ccttggtaca tttcataacg atcaagcaac gtcatgtaag aatccattcc acctaacgaa
    17881 ttccccatgt catgcatata ttcacataag atgaacggtt tttccgcttt attttcaagg
    17941 tagtcaatga tctcttctgg tggcgcatac atgcagcttt ttacatctga aatcgaatct
    18001 tcgaatgctg gatttggaaa attcccttca taatggacaa gtcgatcggg gtctactttt
    18061 ttgaagtaat cgttcatttg tcgaatgttt tcttccgcat aagattcatt tccaagagac
    18121 caaaaaagaa tacttggatg gttttttaat gtttcgaaat tcgtttttgc tcgatcaaga
    18181 acagccgcct gccactgagg tactgcacct ggtacgttgt aagaaggctc aaatgcgccc
    18241 attttttgcc atgagccatg ggattcgagg ttcgtttccg ccatcacatg gattcctgct
    18301 tggtcgcata aataatacca agtgatctgg ttggggtaat gagatgtccg tacgccgttg
    18361 atctttgcat tttttatgat agccaaatct ttttccatat cttctatagt gatggcgcgt
    18421 ccttttttcg gatgccactc atgtcgattt accccgttga taatcatccg ttttccattg
    18481 aagtagatga ttttatcttt gatttcgatt ttacgaaaac cgaaggaata gtcaatatac
    18541 tccgttactc ttttttcttc gtctatcgtc tccaattcaa gcttatataa attgggctga
    18601 aaatgccccc acagtgccac attttcaaat ccatcgatgc gaatcgtcat ttgctctttt
    18661 aacggatatt gctgttgata gatttccttc ccatcaggat ctagtatcgc tacattcagc
    18721 tgactgtttt tggctacttg ctcaaaagac aaatctactt caagtttgcc attttgacat
    18781 ttgtcatcta agcgaggttt gatccaaaga tcttctaaat ggttttgtcc tcgagcaact
    18841 aagaagacat ctctgaagat cccaaaaaat cggaaaaagt cctgatcttc caagaatgca
    18901 gctgtacttc ttttgtaaac ctccacagct agaatatttt cttcttccaa ataaggtgtt
    18961 aaatcaaatt cagacggagt gaacgaatct tcggcatacc cgataaaatg accattcaac
    19021 caaataaaca ttgcttgttc tacaccagca aaatgaatcg agatttgttt tccctttaga
    19081 ccttcttcta atcgaaaagt cgtcaaatac gaaccaaccg gattgtcttt agcagctgaa
    19141 aagatccctt ccgcctcttt ctcagggctt gtcgtgaaag ggggacggcg aaatacatgt
    19201 ccctcccacg gatacatcgt attgatgtaa tggatcttgt cataatttgc taattcaata
    19261 tgactaggaa cagtgatttc atcaaaagaa caggcatcaa aatctttttt ataaaatgtt
    19321 tttggtcgta ccgaaggatt tggcgaataa caaaaacgcc atgatccatt taaagactgt
    19381 ttaagggagg atgtggtttc tacttcttcc cttgttgcat aacaaacatg atcgctatgt
    19441 gcatttgatt ggttcactct aaaaacttgt ggatcatcta accattttat atttgctttc
    19501 atactttttt ccttctctct cataactttt tgaaatgaag cagttctgtt tggaaatcac
    19561 cttgaaaatc ggggaagtaa agtccaactg tcatcaattc ggcccccgaa aatatccgct
    19621 tcgaggtctg ttcctgataa agtgcctcat catctaaccc catcagatat acttcatgga
    19681 acgctggttg agctgaagct agtatcctcc ctaaaaacac aatagcttca tcttgtttag
    19741 gagacacaaa catccatgcg gcaatatttt cttcaaaagg actcgccaaa cgataataat
    19801 ggccatactg gatcaccgga cgaatcgttt gataggatat aatttggtct tttacttgct
    19861 tttcttcttc aggagacagc tttgtcaaat ccagttcata accaaaaaca gcgctcatag
    19921 ctgtttctcc tcttgttttc aacgaagtag atctgtcggt ctgatgattt ggtacagctg
    19981 atacatgagc tgtgatggac gaaatcggat aagcaaggct tgttccatat tggattttca
    20041 tacgctccac tgcatccgtg ttatcacttg tccatgactg cggcatataa taaataaacc
    20101 cggcatcaaa ccgtccacct ccaccagaac agccttccca taagatttcc ggataatcag
    20161 ttgtcaaatg ttcaagtagt tcataaagcc cgagcatata acgatgcgct acttctcctt
    20221 gtctctgtgg gtcaagggta atcgaataga catctgaaag cgaacggttc atatcccatt
    20281 tgatgtagtc aagaggaatc gtatctaaaa tctttctcat ttgtttctca atcgtctgcc
    20341 gaacatctaa ccttgtaaaa tccagtacat gttgtgatct ggaagcagaa ggcattctac
    20401 ctggctcctg cataagaaaa tcaggatgtg ttcgatacag ttcagaatca accgagatca
    20461 tttcaggttc aaaccataaa ccaaatttca atcctttgct atgagcataa tctgctattt
    20521 cacgtaatcc atttgtcaac ttgccttcat actcgaacca gtctcctaat gaagaattgt
    20581 ccgcatctct ttttccaaac cagccatcat ctaatacgaa catttcgatg cctagctcct
    20641 ttgcttcatc aatgatcgcc ttgatttttt cactattgaa gtcaaagtaa gtggcttccc
    20701 agttattgac cagaatcggc cgttcagcaa attgatgacg acctcttgcg attctttcac
    20761 gtagcaactc atggtggatc tgactcattt tgttcaatcc ctgacttgag taagaaagga
    20821 tcatttcagg tgtttgaaaa gactggcctg cattcaattc ccacgaaaaa cgatggctgt
    20881 tgatcccacc gacgactctc aattgattga tctgatcttt ttcaagttcg aaagaatggt
    20941 tgccggaata aacaaattga agtccgatcg cttctccagt attttctgtc gtatgatgat
    21001 gaactaaagc aataaagtta ttcatatggt gactgctcgt tcctcgacgg ctttcaaata
    21061 cttttgttcc agataaaaca gattgccgtg aaatctgtct ttcattgaca tgtgctcctg
    21121 gcaaagcaat gacttcatca aatcgcctag tatgggcaaa gtctagctgg aaactagccg
    21181 ctttttctag tttgattgtt tctccggttt catttcttat ctttacagat cgtgtgatca
    21241 ctgctctatc agtaaatatg gtatagaaca aatcgaaata aatcgctgtt tctctatcct
    21301 taagtgaaat caccaacgtt tgtgcctctg acttgtccat cacataagtt tgaggcaagc
    21361 cttcgatctc tggcttccca tctgtaatat aatggctgtc atatctcaga tccagtaagt
    21421 ctgatccatt cttgcgctga atgatcgatg cgggcgttcg aaagtccatg ctgccatgag
    21481 aagagtattc ttgcggtagt gtatctttcg acagagaccg gtcaggattt aagggaacat
    21541 ttcctgaaaa tccacgatct cttcggggat aatttttatt gtctttgtac tgcttgactg
    21601 gtttgccaaa ataaatatgc gccaatacat tgctttcttc tatctttatc agataagaaa
    21661 gaaatgtatt tcgtaaatgg aagactcggc tcttttcatc aaataagatg aaatttttat
    21721 ctgtttttct atgcgtaata tccatcattt cactcccaac aaaacggaaa ccgtttactt
    21781 ttttttataa ttcctgtata ctgtttacta aacaattttg tcaataaaaa ttttttattt
    21841 ttttgaggag tgtgtaaaaa tggttggaat tagagatgtc gcacgaaagg caaatgtctc
    21901 tcctgctaca gtttcccgtg tgttgaataa cgatcagacg atccatgtgg ctcagactac
    21961 tcggcaacgg atagtagagg cagctgctgc gttggattat gacatcagta ctcgtaaata
    22021 tatcaaaaag agaatgcctt cgattggtgt gatctctacg atcggcaaag cgagcgaaga
    22081 acaggatatc tattacaaac atttgcgagt agggatggaa gaagaagctc gacgtctgca
    22141 tttagggatg aatcgaatct ataatctatc tgataatcct aaacaatggg aagatctaga
    22201 tcagttaggt gcggtgatcg ttgttgggac tgttacgaaa gaatcgatta ccgccttgtt
    22261 aaaacaaaac atgcaaatag tcgtcgtgga taaccctgac atccagcaag aggtcgatat
    22321 ggtgtatgca gatcttgaac ggatgactaa aaccgtactg gaattgttct tagaaaatgg
    22381 tcataaaaga atagcctata tcgggggata taaagtcgat atggatgaat tcggtgggaa
    22441 gcagatttct ataaatgaaa agaggatgcg tgcctatcag cattttatga tcgatcatca
    22501 cttagaaaaa tatatgtctt atcatctagg tggctggaca gaaaaacaag gtgaggtact
    22561 tgcgactaat ctattagaaa atgaatcccc tcttcctact gccattcttg tcgggagcga
    22621 tccaatggca attggtgtct atcgggcatt gaaacaaaaa aatatccaaa ttggagaaga
    22681 gattgttgta gcaagctttg ataatatcga gacaatcgca aacctctccc ctggcttgac
    22741 cagtgttcaa atcaatgcga aatcaattgg gcaagcagct gttcgaatgg cattggagcg
    22801 aattgacggt atacgggacg aaccaatcat tttgacttat cctgcacgat tgattgttag
    22861 ggaaagtttt atcccaagaa caaaaaggta ttgaaagaga ggttgtgaaa agccgcttcg
    22921 tttcttcata ctcaaaacga aacggctttt tcacaacctc ttacttatac ttattcacaa
    22981 aatccaatgt ctgctggtaa caattttgcg attcttttag cgtgtaatca aattggtatt
    23041 catgggtaat ctcttttttt gtatctgtat aaaacaagga ttgcacgggt acctttaatt
    23101 ctgtcaaccg attttctaat gcttgacctt gctcctggaa tgagtatgca tttccgtcag
    23161 ttatatacgt tggcggaaat tccttagaaa catggtcagc cacactagct tctttcaatt
    23221 gctcactcgt tttccaatct tttgttccaa tcaacgacca cgcaacggtt ttgacgaaaa
    23281 acttcataaa tcgatcatcc gaatgctgct gtgctgttag cttcaaatca acaggtccgc
    23341 aataagaaat ggttcctttc agactatctt ttggaagtag atgtttgatt ttcatttgtt
    23401 tagcatacgc atcattcgtt tgtatagctg catattgcaa tgcgatttgt gaacctgcac
    23461 tatctccacc aaaaaataat tgttccatgt ctagcaattt gtcgtctttt tcttcagcta
    23521 ataaatcttt gatcagttca ttcacttgaa gaacttgatt agggtattct gaatcaggcg
    23581 ctaattcata attcatcgca accactgcga tgttggcgtc tgaaactaat tttgtcgcaa
    23641 attctttgac acccgattta tctccgccga cgaatcctcc accatgtacc caaaatagaa
    23701 caggaacagg ccccttgctg ttttttggat agtagatgtc ataagtattc tctttatact
    23761 tggactcata cgtcaaatct gtttttttcg ccacgttttt ttctgcttga tcgaacgttt
    23821 ttttatcagt gatttgaaca gtatttgaaa acatatggtt gatcaagaat gctccaggtc
    23881 tgggggaaag ctggaaggca agaaagattg caaacgcgat cccaagaatg attccgaaga
    23941 accatccgat aaatttaaga attttctttt tcataaggct ccctcagtag ttagtattaa
    24001 gcattctgat tataccatag tgttatttcc tatcaagctc tcctagatga tttaatgatt
    24061 ttccccaaac ttcttctgca atgcttttga caaaagaaat tttttccatt gttcttcaaa
    24121 caaaacattt tcttcttctg ttgaagcaaa tccacattga ggacttaagc acaaacgatc
    24181 caaaggcaga tatcgtgtcg cttcgtagat ccggtcaatc acttgctggt gctcttccat
    24241 ttcgccattt ttcgttacag tcaaaatcta agacaactaa tttatcttca gagactttct
    24301 ctaaaagaac aaaactccca gcacgttcac tatcgtattc taaataatag gcacggacat
    24361 tttcttgagt aaaataatgg gtctgctacg tcgtcatccc ccctgatgcc gccaaaatcg
    24421 aataataatt tccgcgtcaa acatgtatat ttagtgttga atcatttgag aatatgaaaa
    24481 taaaaatctt tttgatcaat cagttaatca aattgaaata acgaaaaatc tgagacagaa
    24541 tgacgttcat tctgacccag atttgatttt gcacaactca acagtcctta ttggcaaatc
    24601 gccaaaataa tgtagactaa ctaaaaagac taagaaacaa taacaaaagt taacacttct
    24661 tagtctttct ttagcacgtc tatttattaa agattttagg gccttttctc atgtttttac
    24721 gatcattctt aggtgtttcc atttgtttgg atcggcctcc accttgtttc tttttgatca
    24781 attctttcat tttttctttt tggtccattt ttattctcct ttaacgaatg aagcgactaa
    24841 ttcgatatga tgggtttgcg ggaaaagatc tacaggctga actttcttca cggcataacc
    24901 tgcttctgca aataacttag catctcttgc aaaagttgcc ggattacatg agatatagac
    24961 gattcgttct ggtgacattt cgattgctga ttcaataaaa tgatgatcta atccttttcg
    25021 tggagggtca actacgatca cagatggttt gatccctttt tcttgccatt ttttcataac
    25081 tttttctgct tttccgactt cataatgaac attttcgatt ttattattta aagcattgaa
    25141 ctgtgcatct tcaattgctt ctggaataac ttccatccca tacaccttat ccactctgtc
    25201 tgctaaagac aagccaatcg tacctattcc tgaataagcg tcgatcacaa tgtctttttt
    25261 atctaactcg gcaaactcga tggctgtttg gtacagatac tctgcttgtt gagtatttac
    25321 ttgataaaat gactttgctg agattcgata ggtttttcct aatagctgat cttcgatata
    25381 gtctctgcca tataaaactt tttctttgtc tcctaaaatc acatttgttt tttcttcatt
    25441 gatattttgg atgaccgaaa ccacttctgg caattcttca tgaatagctt cagcaatttc
    25501 cttggattta aagaattttt ctttacgagt aactaaaacg accatcattt catgggaata
    25561 atgaccacga cggataatga tgtgacgaag ctgtccttca tttttttctt cattatatcc
    25621 tcgtacttgg aaccgttgca agatatctct gactactttg attgcttgat cgatgacagg
    25681 atcttggata taaaaatctt cgatagggac tagatcatgg ctattctttt tgtaaaaacc
    25741 ggtggctaac tgtccatcga tacggcgaac agggatctgt gctttattac ggtagccgaa
    25801 tggttctgcc attccgaccg ttggaagtac aggaacttct ggcatcttag caatcttcgc
    25861 cattacatgc tctacttgtt tttgtttgaa gacaagctgt tgttcatact tcaaatgggc
    25921 taaaggtgcg atacctatgc gaagtaatgc attgttttct acaggctgac gatcaggtga
    25981 ttgtgtaata aatgtttcga cttttccata tccaaactta ttgcctgctt ttactactag
    26041 tatttctact tcttctccag ggagtgcatt ttcgataaat aatggataac catcgatttt
    26101 tgctaccccc attccttcat aactcaagtc gacgatttcg actgtatatc gttcgttttt
    26161 ctttactatt tgctgttctg tcatattttg ctcctttaag cctatttttc aatcctactg
    26221 aactctacta taaatcagct aattacacaa ctaaaaccgg cactgacctt actacgttca
    26281 gcaccggctt ttgattaatt cttttgatta ggtgtttcgt ctgctatttt tccgtcaccg
    26341 tcgatatctt catccgttaa acgttcgact tcttttacga attctttgct gacaacctct
    26401 aattcttctt catctgtacc tgtgattgct tcatcaggaa tctgatcggt attggcaaag
    26461 aactcaatgt gttgatgaag attttggaaa tgcatcggtg cttccccgcc gtattcacca
    26521 tctaagttga tcattactgg tcgattgata tcatcttctg tttcaacgaa caaactgctt
    26581 gttttggtat agattattct tggatcatcg acatgtcgac cgccattcag catgagtgct
    26641 gcaatatgca agatctcaaa gatatttgct gtcttcacaa tgattaaaga aaacttccca
    26701 tcatccaatt tggcatctgg cgcgatctgt tcgaatcctc caacagagtt cgtcagccct
    26761 aggaaaaaca tcgaagcatt tccgatatat tccccttcat catacttcaa gcgcattttg
    26821 attggcttta ctcgagggag catctctgct cctttcgcaa gataagcaag ataaccaaaa
    26881 atactcttta gttctgatgg aacttcataa gtcaattctg tcaaatagcc acctgcagcg
    26941 atattgatga agtaactgtt atcagaaagg ccgatgtcca tttttacggt ttggtgtttt
    27001 tttatcactt ctgctgctgc tttaatatta tctctaggaa tttttaatgc tcgagcatag
    27061 tcgttcgttg taccagctgg gatgatggcc attttaggac ggtgttcaag cggagcgatt
    27121 ccattaacca cttcgttgat tgtgccatcg ccaccagctg ccactactag atcaaaacct
    27181 agttctgcga ctctacatgc ttcattcttg gctgaatctg gttctggtgt agttgcatag
    27241 gcgctggctt caaatcctgc ttcttctaat gcctgtagga tatccgccaa attttttttc
    27301 aataattctt ttcccgaggt ggggttataa attacgcgtg cttttttcat tttcagcctc
    27361 tatcgtttcg ctaattcatc tttaagtagc ttattcacga caccaggatt tgcttgtcct
    27421 ttagttgctt tcatgatttg accgactagg aagccaaccg cacgatcttt tccatttttg
    27481 aaatcatcga cagattgttg gttgttatcc aaaatttcat taatgattgg cagtagtttg
    27541 ttcggatcag ataattgcac ccaaccattt ttttcaacta cttcttgggc ttctccgcca
    27601 tttgtgatca attcacggaa gacttttttg gcgatttttg agctgatcgt tccatcggcg
    27661 atcaaacgaa tcattcctgc taagttctca ggtgtcaatt ttgtctcagc taattccagt
    27721 ttctcactgt tcaagtaagc agaaacttca cccatcaacc agttagatgc ttgcttggca
    27781 tctgcaccat tttccaaaac agcttcaaaa aaatcagaca tttcttttgt aagtgtcaat
    27841 accattgcgt catactctgg aagtccaaac tcacggatat agcgttcgcg acgagaagct
    27901 ggcatctcag gcaaactagc ttttactttt tgaatccatt cgtcatcgat cacgaatttc
    27961 gggatatcag gctctgggaa gtaacgataa tcactagacc cttctttgac acgcatcaaa
    28021 actgttttat tcgttgtttc gtcaaaacgg cgtgtttctt gttggatttc tcctcctgaa
    28081 agaagaactt ttgcttgacg tttttcttca aaagctaaac cttttttcac gaaagataaa
    28141 gagttcaagt ttttcaattc tgctttcgta ccgaactctt cttgtccata agggcgaaga
    28201 gaaatattgg catcacagcg catagagcct tcttccattt tcacatcgct gacttctgta
    28261 aattggataa ttgatcgcaa tgcttctaga taagcgtatg cttcttctgg tgaacgcata
    28321 tcagcttcag aaacgatttc gatcaaaggc gtgccttgtc ggttcaaatc aacgtaagaa
    28381 taaccgccaa taccatgcat gttttttcca gcatcttctt ctaaatggac acgttcgatc
    28441 cggatttttt tctttttacc ttctacttcg atttcgatcc atccgtcatg accgattggt
    28501 tgatcaaatt gggaaatttg atatgctttt ggattatctg ggtaaaagta atttttccgg
    28561 tcaaaatgag tatctttaga gatttcacag tttaatgcca aagctgcttt catcccgaat
    28621 tccagcgcag ctttattcat tactggcagc acgcctggat aaccccagtc gatcacgttt
    28681 gtattgctgt ttggttctgc accaaaatga gctggcgctg gagagaaaat ttttgagttg
    28741 gtttttaact cgacgtggac ttctagtcca atgactgttt caaaattcat tcgtctttcc
    28801 ctcctaaaat agctggttgt tgtttatgga attctgttgc ttgttcaaac gcatatgccg
    28861 cttgatacat cgttttttca tcaaaataat taccgatgat ctgcaatcca actggtaatc
    28921 cttctgataa acctgccggt acagacatac ctggaagtcc agccaagttg acagggatcg
    28981 ttaagatgtc actcatgtac attgtgattg gatcgttaat gttctcaccg attccgaatg
    29041 caactgttgg tgaagatggc ccgatgatca agtcataatc tgcaaagact ttttcaaaat
    29101 ctcgtttgat caatgtacgg acttgtcctg cttttttgaa gtaagcgtca taataacccg
    29161 cgcttaagga gaatgtacct aacatgatac ggcgttttac ttcttcaccg aatccttctg
    29221 aacgtgagtt gacatataca tcatctaatg atttcacatt ttcagagcga taaccgtagc
    29281 ggataccatc gaatcgttgc aaattggaac ttgcttctga tgaggcaatg atataataaa
    29341 cagccacacc gtattttgaa tgaggcaagc tgacttcttc cacaacagca cctaatgaac
    29401 ggaatgtttc tgctgctttc aatacagctt cttttacgcc aggggccaca ccttcgccta
    29461 agtattcttt aggtaaagca atcttcattc ctttgatgtc gcctgtcagt ccctctgcaa
    29521 aatcaggaac tgaaacgcct gaagaagtac cgtctttttc gtcataaccg ctgatagctg
    29581 tcaatgctaa tgcattatct ttcaccgtac gggtcattgg tccgatttgg tcaagagatg
    29641 aagcaaaggc aatcaaacca aaacgtgaga cacggccata agtaggtttc atccctacga
    29701 tgccgttaaa agctgctggt tggcggatac ttcccccagt atcactacct aaagaaactg
    29761 gaatttgtcc agctgcaact gctgctgcag aaccaccaga agagccacca ggtacttttg
    29821 ttgggtccca agcatttttt gttttcttga aataagaagt ttctgtactt ccacccatag
    29881 caaattcatc catgtttagc ttaccaacag ggatcatatc tgcttgatag attttatcca
    29941 tgaccgttgc atcataaatt ggttcaaaat tgtaaaggat cttagatgca gcggttgtca
    30001 agatatcttt tgttacgata ttatccttga taccaattgg aataccagca aggacgttgc
    30061 tttcatcaat ccctttttca tctaaagctt ttgcctgttc caatgctttt tcttcgttca
    30121 atgtaatgaa tgcctcgacc tcaggctctg tttctttgat tcgttcaaat gttgtttttg
    30181 tcaaatcttg tacagtgatt tctttcgata caagcatatg atgcagttct tctagagaac
    30241 gattgtgtaa ttctgtcatt atgcgccagc ctccccatta tcgatgattg cgggtacttt
    30301 gataaagccg tcttctgttt ccggcacatt tttcaataat tcatcgcggt tccatccagc
    30361 ttgcgcccga tcttgtcgca tcacgtttac tgtttcaacc acattcgacg taaatggaac
    30421 accttccgta tctacttctt ccagcatttc aaccatatcg atgattttac ctaattggtc
    30481 ggtaaaactt gcaagttctt catctgcgaa cgcaagtttt gctaatttgg caacgtgttt
    30541 cacttgttct tcactaatcg ccatactacc ccttctttct aaacgagtta aaaactttgc
    30601 tctctatttt aacacaagag tgccgtgtct gaaattgttt attgaaattt gtctgaaaaa
    30661 actcattttt cattcttcta ctgagcgact tttagccaat gcatctgcca tttgatcttc
    30721 tgtccaaact tcgattccta attcttgtgc tttggtcagc ttgctgccag catcttctcc
    30781 tgctaccacg atatccgttt ttttcgatac gctaccagtc actttcccgc caagattttc
    30841 gatcgtctct tttgcttctt cacgagtaaa tcgtgttagt ttaccagtaa ggactactgt
    30901 tttttcttta aaaggagact cgacttcttg caattgggca tttcgaagcc ctttgtattc
    30961 gaaattgacg cctgcttgtt ttaattcatt catcaattca tggacttctt cgttactgaa
    31021 ataagtgact acactgtcgg caatgatatc accgatcgta tcaagcgcaa taatcgactc
    31081 atagtcactt ttactcaatg tttctagatc accgaaatgt tctgctaaaa tttttgctgc
    31141 tttcgctccg acatgtcgga ttcctaaccc gaaaatcaac cgctcgaccg aattatcttt
    31201 gctgttgtcg attgctgtca aaatattatt tgctgatttt tcttttattt tgtccaacgt
    31261 caataactct tcttctgtca atttgtaaag atcagcaaca tctgctacta gttttttatc
    31321 atacatctgt tctagtacgc gcggccccaa cccatcgata ttcatcgcat ttcttgaaac
    31381 aaagtggttc aaaccttctt tcatttgagc agggcattta ggattgatgc accgtaaggc
    31441 tacttcttca tccaaatgga caagttcact tccacaaact ggacaatgtg taggcaattg
    31501 gtattcttcg ctgtctttag gtcgtttatc taacacgact tgcgaaactt ctgggatgat
    31561 gtctcccgct ttataaatca gaactgtatc tttcaagcgg atatccttca acttaatata
    31621 atcaccgtta tgcaggctgg cacggcttac tgtcgttcct gctactcgga ctggtgtcat
    31681 gatagctgtt ggcgttacta cccctgttcg accgactgtc cattcgatgt tttcgattag
    31741 cgtttcaact tcttctggcg gaaacttgta agctgctgcc catctcggtg ctttgacagt
    31801 aaaaccaagt tgatcttgta gcgagaattc attcactttg atgacgattc catcgatttc
    31861 atagggcaaa tccacacgtt tgtcatgata ttcttcaatg tatgaccaca cttcatcaat
    31921 cgaatgacat agacgttttt cccgattcgt gtggaatcca attttttcta gctcttctag
    31981 cgcatcatac tgggtttttg cttgcatagg accgaaatca gctactgtgt ataaaaaagt
    32041 gtccagattt cttttcgctg tgattttaga atctaactga cgcaaagaac cagctgcggc
    32101 gttccgagga ttcgcaaaga tatcttttcc ctctgcttct ctgtcttggt tcaattggac
    32161 aaatgaccgt tttggcatga aacattcgcc tcttacttcg atgttcattg gttcttttaa
    32221 tttgattggg accgaccgga cagtttttag gttttcagtg atgttttctc ctacagttcc
    32281 atccccgcga gtcgcacctc tcacgaaatt tccatcttca taacgcaaag aaacggaaag
    32341 tccatctatt tttagttcac agcaatagtc aactacacgt cccactgctt tttgtacacg
    32401 ttgatcaaaa gctatcagct cttctttgct gaaaacgtcg ttcaaactgt aaagcggaat
    32461 atcatgagtg actttttcaa aaccttctaa tacttttccg cctactcgtt gagtgggaga
    32521 atcagacgtg atcaaatcag gatattgttc ctcaattgct actaattctg cgtattcttt
    32581 atcataaaca tagtcttcca ctgttggttt atcttctacg tagtattcgc gagaccattg
    32641 gttcagtctt gtacgaagtt cttctgctcg tttagcagct tcgtcaatcg tcatttcatg
    32701 ttccatcatt taccctccac tactaagctt tttcaattgg cgcaaaggca gccaaaagac
    32761 gttttactcc ttgacttggg aaagccacat ctagttctag atccttcgac gtcccgctta
    32821 ctcggacgac tgttcctaga ccccattttt tgtgtttgac tttatctcca ggtttccagc
    32881 tgttattttc ccctccagta gcacttttag agggaacaac cgctgtctct ggctgtttat
    32941 agctatatgc tgttttttta gcgaatacag gtgttttttt aggcgtagga cgcatgcctt
    33001 cgatttccag taactcctga tcgatttctt ctacaaaacg agaaggtcgg ttatattgcg
    33061 tccgtccata taatgttctg gagaaagcat ttgttaaata caacgcttct tctgctcgcg
    33121 tgatccccac atatgcgaga cggcgttctt cttccagttc gctttcttcc atcaatgctc
    33181 gagataaagg aaaaaccccc tcttctagac caatcaggaa tacaactggg aattctaatc
    33241 ctttggctgc atgcaaagtc atcaatgtca cttgggaagc atcttcttct aaattgtcaa
    33301 tatcggaaac aagtgccaga tcatttaaga agaccgttag tttttcttct ggtgcatcag
    33361 cgtcttcttc attttgctgt tcgaattgtt tgtcgaattc ttgtgtgacc gttaaaaatt
    33421 cctccaagtt ttcgatgcgt gcttgtgctt ctaatgtatt ttggattttt aagtcttcta
    33481 gatatccgct acgatccaaa acctctttcg tcaattctgt cactgtcaag tatgggatca
    33541 tctgagtgac ttcttggatc atctctccaa atgcacctaa ttgctgtcct gcttttccac
    33601 cgatatttgc cagatccaca ttttgcgctg cttcaagcag tggccattca tgaagagaag
    33661 caaaactgcg tagtttttcg atagatcctg gtccgatgcc tcgtttgggc gaattaacga
    33721 tacgctcaaa actgattgaa tcttgaggat ttgccagcac attcaaatac gctaaaatat
    33781 ctttgatttc tttacgatcg tagaacttgt gccctccaac cattttataa ggaatatttg
    33841 ctttcaacag agtttcttcc attacacgag attgtgcgtt cgtccggtac aagatggcaa
    33901 agtcgccgta attccgatga ttgcttcgaa tctcttcttg catccgatct acgataaaac
    33961 gggtttcgtc tcgttcatta tctgcacggt agtaagtgat cttgtttcct tctttgtttt
    34021 ctgtccacaa gttttttggt tttcgattgc tattgttttc aatgacttgg tttgctgcgc
    34081 taaggatgtt tttcgtggag cgatagtttt gttctagcaa aattactgca gcatctggat
    34141 aatctttttc aaaatccaag atgttctgca tatctgcacc gcgccagccg taaatacttt
    34201 gatctgcatc tcccacgacg cacaggttcc taaatctacc tgccaataaa ttgactagcg
    34261 tatactgtgc gtgatttgta tcttggtact catctacatg aatataatgg aatttgtttt
    34321 gataatatgt gagtgaatca ggatgttctt caaataatcg aatcgtattc atgatcaggt
    34381 cgtcaaagtc catacattga ttatattgaa gctctttttg gtaagcagca taacattttg
    34441 ccgcaatctc ttcgtaataa ctgccttgca tttttgcaaa atcctgaggg gtctgcaggc
    34501 tgttttttgc ttgagagatt gttcctaata tacttcgagg atcatatttt ttcggatcaa
    34561 tgttcagttc ttttagtatc cgtttcatca aggtcagctg ttcagaagaa tcgatgattg
    34621 taaagttccg gttatacccg ataaaatcaa catctcttct taaaatacgc acacacatcg
    34681 agtgaaaagt cgatacccag acatcttcgc ctccagatgc gagaatcgca ttgacacgtt
    34741 ctttcatttc ttttgcagcc ttattggtaa aagtaatcgc taggatgttc caaggattga
    34801 cttctttttc ctcaatcaaa taggcaatgc ggtgtgtcag cactctcgtc tttccacttc
    34861 ctgcacctgc cattagcagt aatggtccgt cagtgtgaag aacagcttct ttctgacggg
    34921 ggttcatccc gtttaataat tcagctttgg atgacattaa caacatcctt tcttttatca
    34981 atcttgttaa ttatagcatt ttcttggctt tcattaaact agctagttca aaatcatttg
    35041 tttcttttta tggatttttc cttgtcaatt cgttgatttc gcgctaaatt aaaagaaagc
    35101 gaggcgtttg aatggaaaaa aaaggaaaaa tcaagaaatt agataagact cagatatatt
    35161 ttattctaaa aggaatattt gttggactgc tgacaggaat cgtcgttagc ctgtttcgat
    35221 tatctatcga aaagctaagc gatttagttc gaagtatcta tgaaatgagt cgggaacagc
    35281 cgatttactt aattggccta gctatttgct gcatccttgc agcatttttc gtgggttatc
    35341 tggtcaaaaa tgaaccggat atcaagggaa gcgggattcc acaagtagag ggacagcttc
    35401 gcggggaatt atcaatgaac tggttctctg ttttatggaa aaagttcatt ggcggtgtac
    35461 tttcagttgg tgcgggcctt tttctaggta gagaaggacc ttctatacag cttggcgcat
    35521 ccattggaca aggagctgga caattgtttc ggagtccatc ttcagaagaa aagattttaa
    35581 tttctagcgg tgcaagtagc ggacttgcag cagcattcaa tgctcctatc gcaggattac
    35641 tctttgtttt agaagaaatc catcactcct tttctccact tgtttggctg acatcatttg
    35701 cttcagcaat cactgctaac ttcgtctctc tttacttttt cggcttgaga cctgttttat
    35761 atctaggtga ccttccttct ttgccgttga aatattatgg ttccttagtg atcttaggag
    35821 tacttctagg catcttaggt tttatttatc agaaggtctt gctggctttg ccaaaatttt
    35881 acaaccgatt gcctttacca gcttattttc acgggttcgt tcctttcttg ctgattttgc
    35941 caattggtta tttatggcct agtactctag gcggaggcaa tcaaatcgtt ttagcttttg
    36001 gacatgcttc cttgccaatc attatgatta tcggtttgtt tgttcttcga ttcgtttttt
    36061 ccatgatttc ttacggtggt ggtcttccag gggggatttt ccttccgata ttgacattgg
    36121 gggctttaat cggtagcttt tatggaaaca ttatggttga tttcgggatg gatccaatct
    36181 acatcaagga ttttgtcgtt attgccatgg ctggttattt cacagcgatt ggaaaagcac
    36241 cgctgacagc aattatttta gtgacagaaa tggttggctc tcttaatcat ctgatgcctt
    36301 taggattggc tgctcttgtt gcttatatcg tcaatgacct tctcggcggt aatccaattt
    36361 acgaatcatt attggaacgt ctacttggaa atactcagac cgcctcacta aaaggtgcaa
    36421 ggacaacttt tgattttcca gtaacggcag agagttcttt agacggatta atggtacgag
    36481 acttcaattg gcctaaaaat atgctgctaa tctctatacg cagaggaaat caggaaatct
    36541 tgactcacgg tgatacgatc atgaatgtag gagatatttt ggttatcttg actgatgaag
    36601 caaacttgcc aagaatcaag caggaaatcg aggctaaatc ttctatcaca aatttgaagc
    36661 aatcatttta acgaacatgg aaaaactatt ttacaacaac tattcatcta cactacttga
    36721 ttctatatga ttctataaaa aaagaagaga ctgggaaaaa ccagcctctt cttttttatt
    36781 tctatgcttc tgctgctgca ggagcttttc tcaataaacc ataaatcaca ccagatacga
    36841 ctgctccgat agcaataaac accaaataca tgaccgggtg actcagtaag aatacaacga
    36901 aaatcccgcc atgaggagcc agtaatttga tcccgaaagc tccgactaat ccgccagtta
    36961 aagcagaacc aataataaaa cttggaatgg cacgaatcgg gtcagctgct gcaaatggaa
    37021 tcgatccttc cgtgacaaac gatagtccca tcacgatgtt cgtcaaacca gcatcttgat
    37081 ctgttttgct gaatttattc ttaaacaaac gtgtagcaac gaaaattgcc aatggaggta
    37141 ccattcctcc agccataaca gaagccatca cgacacttcc gccagttgca acagtcgtag
    37201 ctaaagttgc tgtaccaaac acataagcgg ctttgttgat tggaccgcct aaatctgccg
    37261 ccatcattcc agctaataat gcaccaagta aagcagcatt cgaaccactc atacttgtca
    37321 agaaaccgtt caatgcatca ttgattgctt tcatagggac attgaccagc aacatcaaga
    37381 aaccagtaat cagtaagcca aacactggat aaaagaggat cgttttaatt ccatccaatg
    37441 attttggtaa gttcttgaat aatttacgta aaaatacgat gacataacca gctaagaatc
    37501 caccaatcag tgcacctaag aatcctgcac cgcctgtatt agctaatgca ccagcagcaa
    37561 atccaacgat caaaccaggg cggtcaccga tacttgaagc aataaatcct gctaataccg
    37621 gcagcatgaa accaaatgca gcttgtccga tttggttgaa ccagctagca gcttgattgt
    37681 aattccctag atttgataat tgatcatgtg gcacgccaat aaattggtcg atcatgaaag
    37741 aaagcgcaat agcgattcct ccaccaataa cgaaaggtaa catgtgtgat acgccgttca
    37801 tcaaatcttt gtaaattctt tgtccgatag taccatccgc atttccttct tctgaactgc
    37861 tttcttttcc atcaccatgg aatactggtg cagtaccact aagggctaga ttgatcagct
    37921 cttctggttt acggatacca tcactgacag gacgattgat cagttctttt ccgtcaaagc
    37981 gattcatctc tacttttttg tctgctgcaa cgattacacc ttctgcacgg gcgatgtctt
    38041 cagcagtcaa tcggtttttg attccttctg aaccgttcgt ttcgactttg atctccacac
    38101 ccatttcttt cgcttttttc ttcagagcat cttctgccat gtaagtgtga gcgatccctg
    38161 ttggacaagc tgtaacggca acaacgaatt ttctttctgg atgagcagaa gcttctgcag
    38221 cttgtgtttc ttgttctgct ttttcttctg cttctttttg ctcttcagct gtttggaaca
    38281 agtcttggac ttcttctggt gtatttgctt cttttaattt tcctacgaaa tctgcatcaa
    38341 tcagcaatcg agatagtgct gctaatgctt gcaaatgtgt gtcgtttgcg ccttctggag
    38401 ctgcgatcat gaaaaacaga taagttggct gtccgtctaa ggcttcataa tctactcctt
    38461 tttggctttt tgcgaataaa actgttgctt ctttaactgc actgtttttc gcgtgaggca
    38521 tggcgattcc atcaccaaga cctgtagaag tttgtgcttc gcgggctaga attccttctt
    38581 tatatgtttc gatatcggaa atccggccgg catcaaacat tttttggacc atttcatcga
    38641 ttgcgccttt tttatcagtc gcttgcaaat ccatgatcat cgcttctttg accattaaat
    38701 ccttgatatt catgattatc ctccttgaaa ctaatcaatt tttgtgattt caacttctgg
    38761 cagcaactca tcaatcaatt cgcgagctgc caagtcatca gaaaaagctg ttgcgcttcc
    38821 gcaagcaact ccccatttaa aggcttcgat tggatctttc gtacggctgt aagccccaat
    38881 aaagccggca atcattgaat cccctgcacc tactgaattt ttcaatgggc gaactaatac
    38941 attcgtttta taagttcctt gttccgtgaa gagcaaagca ccgtctccag ccattgagat
    39001 cagcacattc tgtgcccctt cagctagcaa ttttttacca tacggaatga tttcttctac
    39061 agattggaag gttgtattgt aaagctcagc taattcgtga ttgtttggtt tgatcaacaa
    39121 tggtttctta acaagtgcat tttttaggtc atcacctgtt gtatcaatca cgaaatctgc
    39181 acctttttct cgaatgatat caatcagttc ttgataaaat ccttttcgca ggctagccgg
    39241 cgcgcttcct gacaatacga cgatatctcc ttgtcctact gttcctaatg cttgcttcaa
    39301 ctcatcgatt tcttcatctg aaatagatgg gcccaaaccg ttaatttcag tttcagtatc
    39361 tgattttagt ttgatattga ttcttgtatc ttcttttacg gttgtgaaca aagactggat
    39421 ttgttctttt tcaagccact tcgaaataaa ttcgccagtg aaccctccta aaaaccctag
    39481 tgctgtagat tgagaatcga tccgtttcaa aatacgggaa acattgatcc cttttcctcc
    39541 aggtaacttg aagtcttcct tcattcggtt caaatcgcct agttttaacc catctacgcg
    39601 tacgatataa tcaattgatg gattaagtgt tactgtgtaa atcattgtat tgcctcctta
    39661 attgttgtct tttcacggaa ttcattaacg aattctaatg gacaacgctc ggttatgatc
    39721 gtggctgctt ccaaaggtgc aacagaggtg aaggacaact ggttgaattt ggtatgatca
    39781 atcaatacaa atgtctcttc ggaatttttc atggctaaac gtttaacagc tgcctcttct
    39841 gggtccggag tcgtgaatcc ttgttcaaga tgggcaccgt tcattcccat aaatgcttta
    39901 ttaaaatgaa aattttttag ttgttctatc gcattagatc ctaaaacagc attagttgcc
    39961 agctttatcg ttccacctaa aataatggta gcaagctcca aatcaactaa atttgccgca
    40021 tgttgaacgg aatttgtcac tactgtgatc tgttttcctt gcagaaaagg aatcatttct
    40081 aacgtagtag agccagcgtc taaataaatg acatcttcgt cattaatggt ttcagctgcc
    40141 gatttggcga tttgctgttt ttcgtgaacg tttttgagtg atttttcact catattctgc
    40201 tcaaaattca ataattgctc tttcttagct ccgccatgaa ctctttctag aaacccacta
    40261 ttctccagct cttgtaaatc acgtcttatc gttgattcag aagcattaaa aaggtttaca
    40321 agatcttgtg atttcacgat tttctgctgt tcaagcaagt ctaggatttt ttgtctacgt
    40381 tcctctgtaa gcattttcac gatcacctca atacaaataa tagcatattt tttttctact
    40441 cgcaatataa atcagtcaaa aacgttcata aaaattataa atcagtcaaa aaaaaataaa
    40501 aaaccagtca aaaagactgg ttttttattt ttttgtctat catcaagccg ttttatcgtt
    40561 ttttattttc gcttcttgtt tttttcatat aaacagaagc aggttttctt tccttatgaa
    40621 gaagataata ataaagtccc atcccgccaa acatgaccaa aaaaccgatg actgatcgaa
    40681 taggaaaatt aggaacaaaa aagagacaga cggataataa tgatgccgga accattattc
    40741 gataatagag ttgtttatcc attttcttca cctcatattt tttgtaaagt aatcaattgt
    40801 tctaattctc ggtaatctaa aaattgcaca cgcagatgct gctcaccttt cttgacatcg
    40861 aattggatac ttcgccgctt tttctttatt cgtttgaagg taacatcaga gagtctatgt
    40921 ttgatttcct ttaacggtaa ttctgacagg gattctgaag gaacatagat tcttaaggat
    40981 aaaagatact cttgatattt ttcatctgtt acctctactt ctagatattc cctaatataa
    41041 taacaataag cttcgattcc attatccaaa gcaacagtcg gatcgataac tttatagcac
    41101 atcatgcgcg agcgattctc gtcaacccaa ggaaaatacg cctgagcatt tagtgttccg
    41161 cgaacacgcc tttctttata atctccttgt tgaatatcat aactttttgt tacaaaaaat
    41221 gaagcattct ttttaggaac aggttgcatt tgtgcattct gatactggac agattggatc
    41281 gtatggtcag tgtaaatgta ttcaacgaat ggcggtttca ataaaccgac accattgtcc
    41341 caagcaagat gatcaaaccc aagctggtcc ctaaaatagg attgaaacat ttgattgttt
    41401 ggcttttgcc agttaaaggg gtctcgataa agctggatac atatgacttc gttttctaat
    41461 tccttcactt tttctacttg ttggatgtcg agaaaaatcg gtttagggat gatttgatga
    41521 taatagcgac tataatacat ttcccaacac gaagtgaaac ataaaccttg gtaaaaaaga
    41581 tcgtatcctg gaaattggtt gcaatcgact actacttccc cgttttgttg tttcatttta
    41641 ggtaaatgtt cgatttcatc ctttttctca aacaatagcc gttgagagat ctgttttgta
    41701 ttgtgataga taaactcgct ataagctcgc atataggcga acaccccatg agcaatcatt
    41761 tttttctgaa tgaagtctcg aatcaaaaat tgcttttctt tgaaaacaga gatagacaat
    41821 acatttttta tcaataattg attatcttcc aagcgaaaac tacattcgtt ctcgtcattt
    41881 cctacaacta accaactttc gtctcttacc gatgctagca ttattttttc ttgaagcttt
    41941 cgaagatcat acggatccgc ttgttgtgtg aaagagcgga taaatgttgg ctggatcaac
    42001 agacgtgtca aaatagccca atcgcttatt tcctgttttt tttcttcttc atttaagtct
    42061 gtgagctgga tagtcatcaa taatttatct tcgccaatca ttttctcacc tctttaaaat
    42121 ctttttataa tttaattata accaacgata aagaaaaact aaaactttat gtcttagaaa
    42181 ccaatttgga cgattacatg atagactaag taaaaagaaa caaagaggtg tttacaatgg
    42241 ggttgaaatt tgaacgtaca gataatttag ataaactttt cgaatcattt gctgtcgatc
    42301 cggaaaaaac agttgcacca aaagaagaga tcgatcgatt tctaaaacca cagccacaaa
    42361 atgaaaagaa aaaagacaag aaacaaaagt aagttaagct caaaaaaaga cttaccagcc
    42421 aaacttttat gaaagagttt gactggtaag tcttttttat tctttttatg attcaattgc
    42481 ttgttcgatt gctgcccacg cttcttcttt tccctgtttt gtaacggaag aaaagagaat
    42541 aaacgtgtca ttgcgatcaa aatccaacgc ttttttgatt tcgctctcat gcttgttcca
    42601 tttaccacga gggatcttat ctgccttagt ggcaacaaca attacaggga tatcataata
    42661 cttcaaaaat tgatacatct ggatatcttc ttgagaaggc ttatgacgca aatctactaa
    42721 agaaacgacc gcacgtagct ggtctctagt cgttaaatat gtctcgatca tctttcccca
    42781 ttttgctcgc tcagttttgg atacttttgc atacccatat cccggaacat cgacaaaatg
    42841 taatgcgttc tcgatcaaat aaaaattcaa tgtctgggtc tttcctggct taccggaagt
    42901 acgggctaaa ttctttcgat caatcaatgt gttgataaaa gaagatttcc ctacatttga
    42961 tcgtcctgcc agagctattt ctggcaattg tgtatctgga tattgttttg gtgccactgc
    43021 acttatgata atttcagctt gatggacttt catggtctct ctccttaaca aggtgatgaa
    43081 taaagcaagc ggctgtcgca aaagccaaaa tgacttttac ggcagcctct tttcatcaac
    43141 ccgctttttt ctctgaatct ttatagatta ctgttggctc accagacaat tcagctgctt
    43201 cttttgtgat aatcactttt tcgatgtttt catttgaagg gatatcaaac atcacatcca
    43261 tcatgatttc ttcaatgatg gaacgaagtc ctcgagcacc ggtattgcgt tcgattgctt
    43321 ttttcgcaat ggctctcaac gcttcaggtt cgaattcaag ttcggtatta tctagagaaa
    43381 gaagtttttg atattgtttg actagcgcat ttttcggctc agtcaagatc cgtaccagat
    43441 catctgttgt caatttctct aaagctgcca taacaggtaa tcgtccgata aactcaggga
    43501 tcaatccgaa ttttagtaaa tcttcaggaa tgatatgctg catgacactt tcgtcttcag
    43561 aaagtttttg gttttgtgta ccaaatccaa tcgtcttttc gcccatacga ttcttcacaa
    43621 tcgtttcgat gccatcaaat gcaccaccta cgatgaacaa tatgttcgtc gtatcaatct
    43681 gaataaattc ttgatgtgga tgtttacgtc ctccctgagg tggcacgctt gcaacagttc
    43741 cttctagaat tttcagcaag gcttgttgga caccttcgcc ggaaacgtca cgagtaatag
    43801 atacattttc gcttttacgc gcaattttat cgatctcatc aatatagatg atcccttttt
    43861 ctgcacgttc cacattgtaa tcagcagatt gaagcaattt aagcaaaatg ttttctacat
    43921 cttcccccac ataaccagct tctgtcaaac ttgtcgcatc tgcgattgca aaaggtacat
    43981 tcaatgtttt tgctaatgtt tgtgctaaaa atgttttacc agaccctgta ggtccgatca
    44041 agcagatatt gcttttttgc agttcaacat catctgtgtt ttcttgtgct tgctgattga
    44101 cacgtttata gtgattgtat acggcaacag ctaacgagcg ttttgcgcga tcttgtccaa
    44161 tcacgtagtt attcaatact tcaagaattt ctagtggttt agggacttca gtgaattcgc
    44221 gtacagcttc ttcataaaat tcttcatcaa tgatttcttt acataggtcg atacattcat
    44281 tacagatgta gacccctgga ccagctacga tctttttcac ttcttcttgg gttttcccgc
    44341 aaaaagaaca gcgtacggtc tcattgctac ttggattgtc atacatggtc ttcacccctt
    44401 aacttgcaaa aagtatgcaa gaaactatca aagactttac agtcttttac tatcataaca
    44461 tagtttagta aaaagaaaaa gtgtttaaca aaggatcaac tgacctatac ctttgcattt
    44521 tttactctta acaaaaaata aggaactgtg acaaaatcgt gtcacagtcc cttatttcga
    44581 ataaacaatt ttctagaagt agcttcttca gaaatgattt attacttgat ttctattctt
    44641 tcgcttctgt cgctgtacct gtgatcaatt cgattgcttt tttcattgtc acatcatgtt
    44701 ccagcatatc ttctgtcaac acgcgtttga tttgttcgat tggcatgttg tatgtttctg
    44761 ataattcttg gatttctgca ttgatttctt cttctgttgc ttgaagattt tcagcagctg
    44821 caattgcttc gatcacaaga tttgttcttg tgcgcatttc tgcttcacct tcgaattgtt
    44881 tatgcaagtc ttcttctgta gaaccagtta attgatagta catttcagga gagatacctt
    44941 gacgttgcat gttgttcaag aattcgtcca ttgaacgatg tacttcatca tgaaccatca
    45001 cgtgtggcaa ttcaacgatt tctgcgtttt caacagccat acggatcgct gcttcatctt
    45061 ttgcatcgtc cgctgctttt tcttttgttt ctactaattc tttgcgatat ttttctttca
    45121 attcgtctaa tgttgatact tcgtcatcaa catcttttgc gaattcgtca tctaattcag
    45181 gcacttcttt tgcttttact tcatggattg tcactttgaa gactgcttct tttccagcaa
    45241 gatcttctgc ttgataatct tctgggaaag ttacatttac atctagactt tcacctgatt
    45301 tatgaccaac taattgatct tcaaaacctg gaatgaatga gcctgaacct aattcaagtg
    45361 agtaattttc gccttttccg ccatcaaacg cttcgccatc tacaaagcct tcgaagtcga
    45421 ttactactgt atcgccattt tcagctgcag catcttcttt gataactaat tcagcttgtg
    45481 cttcttgttc acgtttgata cgtgcatcca cgtcagcatc tgttacttca cggtcttgtt
    45541 tttcaacaac tagatcttta tattgtccta attttacttc tggtttcact gttacttcag
    45601 cagtgattac ccagtcttga cctttttcca tgctttcaac atcgattttt ggttgagaaa
    45661 caggatcgat gccagcttct ttgacagcag cttcataagc ttctggtaat acagcattca
    45721 atgcatcttc atataaagct tcttcaccgt acatacggtt gaatacttga cgagaaactt
    45781 tccctttacg gaatccaggt acgttcaagc tttttttcac tttattaaac gcagttgtta
    45841 atcctttttg gatttcgtct tgcgcaatcg taaattttaa aacaccatca ttcgtgcctt
    45901 ttttttcaaa tgttgcagtc atttaattcc ctccgagttt agaaattcat tttatacatc
    45961 aaaaacgttt catgcatacc tttaaatagt acactaaccc tttcaaattg taaacatatt
    46021 gaatggatag aatccttgtt tttcataaca tttttatctt tttcactgat aaaatctaat
    46081 ggaagaagat catttgctgc aaaatttttt ctcttttttt taaaaaatct tgtttttcat
    46141 cttctgtacc ttcatacatc gtttttcctg tacacgcctc tacagtcagt tgaaaccagc
    46201 gcttcgggtc ttcgatgtat gtatcataga caggataaac aattgcagat tgcaacgtga
    46261 attcttcatt taacacggaa ctaagcagcg catcttcatt accgtaacga tctgaaaata
    46321 cacataattg ctggtaactt gcctgctgct gcggctttgg cagatctttt ggtatgactt
    46381 tgactaactt ttcttcaatc gtcaaataga aaacaggatg gcattcgttc aattgcctaa
    46441 gtgtctccag taattttgcc cttgctaaaa tatggacctc tggcgataca ataagtttac
    46501 tcgctgtttg gataaactca tgataaggaa gctgttcgat ctcatcaata gacgcaagct
    46561 gacgtgcagg ttccaacgaa ggaagttcct ccgatttatt gtggagtgcc ttgatcttct
    46621 gcctctccat ttgtccggat aattcttctg caaattggat tttttcaatg acccgctgct
    46681 cgaaagcttc ataaaaatct gtacttgcta tcaattttct agcatataaa aaatcttgtg
    46741 tactgattaa caagtcaaaa taaaattcag caaactcctc attttgaaga tactgttttt
    46801 catgcaagag tgcttgcttc aatgcttctt ttttctcgtc caactcgtaa agacaaaaaa
    46861 caagcaggct attagcctca aagctttctt tcaactcgta agctcgttga aataattctt
    46921 ttgcctgctg atattgctgg tttgctagtg cttgttgtgc tactcgcatt aaattctcat
    46981 atttttctgg aaaatcaatc ggcttcacgc agtgtcctcc tcttgctcat gttcagctca
    47041 tgttcaatag tagcaaaaca aaaaaagcat gacaattaaa aataactgaa gcaccctcta
    47101 aaagctagat tttagatctg acttttagaa ggtgctttca actgttactg ctttttattg
    47161 ctcatcagct gttgacttcc tggttgaatg ctttgatcag attgatcgta acaacacttg
    47221 tattagtgaa aaaatcgcct agatgttgct ttttctgatt aaagattgct ggaagatacc
    47281 ctaacatcag caaaggaaat tttagaatga accggcaagc accttctcta actaaaacgg
    47341 ttgaccagct aagttcttct tcatcaaagc tgatgactcg aataccaaag atcattttcc
    47401 caatcgtttg accatgattg aatttcgtca ataaaataaa atacgctaga taaataagta
    47461 aggctgtgaa tccgtagata ctcagataag aatctgaaaa attaagccaa cctagatttt
    47521 tggcaatacc aagtgttaaa cgcgtaatag ctccgataca caaaagatcg accaagaaag
    47581 caaacattcg gataaaaaaa cctgcataga aatacttagg aaaatcattt gcttttggcg
    47641 gttcatttct tgtgtccgta tactttttcc atttacgttg tttctttttg atttcttcgg
    47701 gcgtcaaagg ctctctttca aaagagctca gtaccttttg ggaaaagttg gagtcaggtc
    47761 ctacagcagg ctgctgttgc ttatcttttt cttgttcctt attttcattt gaaagctctg
    47821 acatgcttac tcacctccgt aataatacat tgcttttggt gattccgctg tccctagatt
    47881 ctcaagaacg gataagatct gactagtctc agaaggcttc aatccctgga attctgctag
    47941 tttacttcct aaccatgtac tagcaaaccc agatggcgtg gaagaatatt ctactagttc
    48001 cgcatcttca agctgttgtt cctttcgcat agcttttaaa gcatcttcag gaaatcccaa
    48061 ttcatcgact aaacctactt cttttgcttg gacaccatca taaatccttc catcagcaat
    48121 ttttttgact tcttcttcag atttattccg tccctcgctg acgattgcta caaaacgact
    48181 atatgcgctg tcgatatatg cttgaagcac cgcttcttct tcttttgtct ctggtcgcgt
    48241 tcctgaaccc atatctttca acgctccgct tttaactgtt gtatcttcca cacccagttt
    48301 ttctagaagt cctgaataat tcaatcctga catgatcaca ccaattgagc cggtcactgt
    48361 ttcttctgtt gcaaagattt tatctgcttg agcagaaata tagtagccac cgctagctgc
    48421 catgtttttc atgctgacat acattgggat atcatgttct ttacgaatcg tatccaattt
    48481 cttagcgatt tccgcacttt cgtagacacc gccaccagga gaatttactt ctaaaaagat
    48541 acctttgact gttggatctt cttcaatcgc cttcagttgt tccataaaat ttttatgatt
    48601 gtaaccttca gaagcaaata aacctgaaga ccctcccgca gcaatcgttc catctactga
    48661 taattttacg attttttgcg tactgtctcc ctcttccaac acatttgaag ctagttcttc
    48721 atccccatat aaccaactgt tcaatccctc aatttgatcg ttttcctgag gcttagtcaa
    48781 actagctgaa actaacgaaa ccacaaataa tcctgcagca atcagaactg ctgcccaacg
    48841 tctcttattc attctttctc ctttctaata caagtttttt atgacgatca cagtttctga
    48901 ctgctcagaa gtgtcgaacc gctctgcttt ttcttcccgc gcatacccgg aaaacgactc
    48961 ttttgtcaat tcatcttcta ccgtaaactc acgagcttca gcaacttcca aggcatcagg
    49021 tgcgatttct tggatgctat cccagccagt atctaaaaat attttcgtat catctgatac
    49081 accagctgat gcttcgaatt tggacaattg tttttttaac attcccagtg tcatgatctc
    49141 aatttttgaa tccattttta ctcctcctct atccgattac tttctattgt accgaactcc
    49201 tgaaaaagat gatagataaa aacccattat tttttgacat aaaaagagca tttgacaaag
    49261 catttgatat ataccaattt attttttatt ctttttttgt tgacaagaac attcgcaaaa
    49321 ggttatgatt ttaaatgttt ccagataaaa aacaaaagag gaggatagtt atacctttta
    49381 ggatagcgcc aggtctgtgt aaacagatga cgaggagagg gtttatcgaa agattcggcg
    49441 gatggcccta gggactgcac tctacagaaa atccacaaaa aatatctgca aagataaaac
    49501 aaaaggattt tcctgcagct ggaagcattc atacgaattc attatagaaa gtaggatgtt
    49561 ttttatgtgc ggaattgttg gaatgattgg attaaaaaat gtaacaccag gattgattga
    49621 cggtctagaa aaattagaat accgtggtta tgattcagct ggtatctttg tctcagatgg
    49681 cacaactgat tatctggtca aagcacaagg cagaattcaa aatttgaaaa acaaaataaa
    49741 tattgatact acaggaaata taggtattgg tcatacacgc tgggcaactc acgggcaacc
    49801 ttctgaagaa aacgcccatc cacatacttc ccaaagtgga cgcttcgtgc tcgttcacaa
    49861 tggcgtaatc gaaaactttg aagaacttaa aattgcttat ttagcaaatg accactttat
    49921 tggtgagacc gatactgaga ttatcgctca tctaattgaa actttcgcga aggatactac
    49981 aacgcaagag gcgtttttaa aagctttgcg ggtcatcaaa ggttcttacg ctttcgcttt
    50041 gattgatcgg acagcacctg atgttattta cgtcgctaaa aataaaagtc cactattaat
    50101 cggactcggc gatggattca atgtgattgc cagcgatgca atggctatgc ttgctcatac
    50161 gaaggaattc gttgagattg aagatgaaga gatggttacc gtcacatcag aaaaagttat
    50221 tattcaaaat tttgcaggtg acatcgtaga acgttcttca ttcgaagcac aagtagattc
    50281 ttcagatatc gagaaaggta cctatccttt ttatatgctg aaagaaatcg acgaacagcc
    50341 tattatcatg cgtcgtatcg ctcaaaaata cgtaacagaa gacaatcgtg tctctcttga
    50401 tcaaaagctg gtcgatacat tgtctgatag cgatcgaatc tatatcgttg cttgcggaac
    50461 tagttaccat gcggggttag caggtaaaca gactctggaa aaattgacac agatccctgt
    50521 tgaggttcat ttagccagtg aatttggcta taacacacct cttctttcaa aaaaaccatt
    50581 ctttattttc ttgagtcaaa gtggcgaaac agcagatagc cgccaagttc tggtaaaaat
    50641 caaccgtctc ggctaccctt cacttacgat tacaaatgtt gctggttcta ctctttcgcg
    50701 ggaagcatcc tttacactgc tattgcatgc tggacccgaa atcgcagtag catcaacgaa
    50761 agcttatact gcacaaatag ctgtactaac tttattagcc aaagctatcg gtgataaaaa
    50821 agaatttgaa acttctcttg tattcgatat cactcatgaa ttgagtttag ttgctaatgc
    50881 gatggaatcg gtcatcgccc aaaaagatta tttagaagag ttggcagaag tatatctcag
    50941 cgatacaaga aatgcctttt acattggacg tggcgctgac tacaacgttt ctcttgaagc
    51001 tgctttgaaa ttgaaagaaa tcagttatat ccaggcagaa ggttttgccg ctggcgaatt
    51061 gaaacatgga acgattgctt tgatcgaaga aggaacacca gtgattggaa tcatcagtga
    51121 agaagtgact ggtgcgcata ctcgaggaaa cctgaaagaa gtcgaaagcc gtggcgctaa
    51181 aacactagtt attgtctcag agggattaga aaaggaaagt gaccagctgg tgctaccaac
    51241 tgtccaccct tatctaacta ctctaaccac agtcgtaccg actcaattac ttgcatatta
    51301 cgcaacgcta ttacggggat tcgacgttga caaaccacgt aatttagcga aatcagtgac
    51361 agtagaataa taaaagaaca aggaccagtg tcagacatct gacactggtc cttgttcttt
    51421 tattttttat gaaaatatct cttaatccag tccgatttct tctttgacta ctgccgcgat
    51481 tttatcgaca tagtaattaa ctttctcatc agtaggcgct tctgccatca ctcgaagtaa
    51541 tggttctgta ccggaaggac gaactaggat tcttccttct gatcccattt cagcttctgc
    51601 ctcttcaatc acttttttga ttgccggaac atccatcgca ccgtttttat cacttactcg
    51661 gatattcact aatttttgcg gatagattgt tacctctgct gcaagctctg ataatttctt
    51721 acctgtctgt ttcatgatat tcagtaactg aattcctgaa agcatcccat ctccagttgt
    51781 gttaaaatct aagaaaatca tgtgcccaga ctgctcgccg ccaaagttat aatcgttttt
    51841 gcgcatttct tctacaacgt aacgatcacc aacttgtgta acgacatctt tcaaaccgat
    51901 tccttccacc gctttgtgga atcctaagtt actcatgaca gtcgtaacga tcgtatcttt
    51961 tttcaatcga tttttttctg ccaagtattt agcacagata aacatgattt tatcaccgtc
    52021 gacaatgttt ccaagttcat ccaccgcaat aatacgatcg ccatcgccat cgaaagctag
    52081 tccagcatct gctccttttt caacgaccat ctcagctaaa cgttctggat gggtagagcc
    52141 tacgccatca ttgatattta acccgtctgg atttgtgccc atggtataaa aatctgtttc
    52201 aagatcggca aacaaacgat taacacttgt tgaagttgct ccgtttgctg catcgacaca
    52261 aatggtcaaa ccggaaaggt caccgttgat cgtttgtaca aggaattgag aatatttcaa
    52321 taagccttct ggaaattctt ctacagttcc caatccttca gctgatggac gaggcaattc
    52381 atccacttca gcatctaata atgcttcaat ttctagctct tggtcatcta ctagtttaaa
    52441 accatctgca ccgaaaaatt tgattccatt atcttgtgct ggattatgag aagcagaaat
    52501 catgacccct gcgcttgctt tttgcaaacg tgtcagataa gcaacacctg gtgtagagat
    52561 cacacctagc tggaacactt cgatacccac cgataaaagt cctgaaatca gcgcttcttc
    52621 taacatttga cctgagattc ttgtatcccg ccctactaac acgcgtgggc gttttccttc
    52681 ttcttcatgc tggcttagga cataacctcc gcaccgtccc agcttaaatg caagttctgg
    52741 cgtaagttcc ttgttggctt caccgcgtac gccgtctgtt ccaaaatatt tacccatagt
    52801 tgtaaaatta ctcctttaac ttaattttct gaggaatttg ttctgctcat ttgtgttgat
    52861 tcgcttgttt ctgatgtttc ttctgtactt gtggtcgagc tgcttgtccc agattccgaa
    52921 ctggaagaaa tggttgttgt cgatggttcc gttcctgaat cagcagatga aatacttcta
    52981 ctcgtacttg tattttcttg tgtccgactg ctcgtcgaag cagagctact gcttgacgtt
    53041 tgcaacacgg gtgtaatctc cacactaact gattcgggct tgatacttaa cccctcttct
    53101 actggtattt caattttacg agttgttcga tgattgatac ctgagacatt gatcggtaca
    53161 gcaatactgt caccgatcga tgcaagcaaa tcgctagagc cgcttacttc tgcttccagt
    53221 tttgaaaaac tgaaatcata gctttctacc ccggcagctg gtgttccttg ctgtatccca
    53281 taaagtcgta ccgacttgct tggcttgatc gaatcagctc tcactgttac ttgcactgga
    53341 tcagagacga tacttaatac ttcccctgct gcattcagcg cctgtattgg gacctcacta
    53401 ttaatcccat catctgtgac catcgaagcg tccacatcag ctactactcg tttgatttct
    53461 gccaatgttt tgtcacctgt agtgatagag acttttttag gactaacgga agtatcatca
    53521 agttcatatc cagatggtgt gaacgttgaa ggaatcaacg tttccacatc gaaatctttt
    53581 attacctttt tctcaaccgt tactgtaata gtcgatggtt cgatctttgc tgtgacagca
    53641 ctgctcaaat tttgaatttt tagcggaacc tcatgtgttc cttcgcccag cttagtgata
    53701 tctgctgtga cgcggaacat ccgtgtatct tcgtttgcct ctgcattcaa ctgaactcgg
    53761 tttgcactgg aaagtttgac agtcactgca ttttcatatc catggatata gtagcgcttt
    53821 gaatcataag aaatcttgat tggcacccca gtaactgttt gattatacgc ttcatttcca
    53881 gaaagcgtat ttggaacgga ctggccattt gcattgaagt acagcgcaat actgaacagc
    53941 aatgcaataa aactgtaaag aatgcttgtc cgtttctctt ttgagatcat ttgcgatccc
    54001 ctcccttagt cataccgtct aggaaagatt gaatcacgtt tttcttgttt tttgttttct
    54061 cttcagcaat cagttctcta cgcaaaattg ccaaatattc ttgctgggat aagccagcca
    54121 tcagttcatt atctaatgta atgctgacat ccccagtttc ttcagagaca acgatggtaa
    54181 tcgcgtcgct gacttcgctt ataccgacag ctgcccggtg tcgagtcccg aattcttttg
    54241 gaatcaacat actttctgat agaggaagat aggcgcttgc tactgcgatc tttccttctt
    54301 ttacaataac tgcaccgtca tgcagcggtg tattaggaat aaagatattg atcagcagct
    54361 ctccagtgat atctgcatct aatgcaattc ctgtctcaat atactcatct aatccagtat
    54421 gccgttcgat agtaattagc gcaccgattt ttcgcttgga catatattgg atggctttgt
    54481 caaaagacaa gatcatcttc tcatcttcct gctgttcgct ttttgattgt ttgaaaagcg
    54541 aactgcgtcc aagatgttcc agtcctctcc ggatttccgg ctgaaaaatc acgatagcag
    54601 ctatcacccc gtacgtaatg acttggttca tcagccaaga caaggtgtgc agaccaatga
    54661 tttccgctac gattcggata acaataaaaa tagctacacc tttcagaagc tgaatcgctt
    54721 ttgtgcctcg cagcagttgg atcagtttgt atacaagata ccagacaacc agaatatcaa
    54781 gtatgttcac caaaaaattc agagagaaca agtcaccgga aaacatttgc tgccaatagt
    54841 ccaaattaaa aagttccccg atttgaaatg acataaaaag cctctcttct cttttgagat
    54901 ttcctctcta gtatatcata tgttttaatt ttagaggctg atctgctcgt atttttcttg
    54961 agaaaaacgc ataattttca ggaaaattgc aagtatgcgt tttatttttc tctgcaataa
    55021 atatttttaa acttcagaga attattcatt atattttttc tgttattttt cctttttttc
    55081 ttttgtagct tgttccattt ttttacgtcg ttctgctctg gcagctcttc tttcttctgc
    55141 ttcaaggtcc gtcgtttttt cttttctagt cttaacgggt acatcgatag attcctctgg
    55201 ttcaagtcgt ttcttgattt tttctaattc tcccgtcagt gcagcatagt tgtaaaaacg
    55261 tgttttagcg tcttggatag tcttttcaaa taaagctttt gcaagatttg gatttgcgtt
    55321 ctctagcgag gaaaagcgaa cttgtttcct catgaaatca ggcatagcag agaaatcagg
    55381 ttttttgtag tccaaagtca ttgggttctt gtttttttcg accattgcag ggttatatcg
    55441 atataatgac caatagccag aagaaacagc ttcttttgct tctttaagcg attctttcat
    55501 tccaccaagc aaaccatgtg tgatacaagg cgtataggca atgattaaag aaggtccagg
    55561 atacttttct gcttcttcaa aagcctttat cgtctgcatt tgattggcac ctgaagcaat
    55621 ttgcgccaca taaacatttc catatgtcat cgccatcatt cctagatctt tctttgaagc
    55681 atactttcca ctagccgaaa atttagcgat tgccgaagct ggtgttgctt ttgaggtttg
    55741 accgccagta ttggaataga cttcgttatc caaaacaaga atattcacgt ctgctccgct
    55801 ggcaagtaca tgatcgatcc cgccaaaacc aatgtcgtaa gcccatccgt ctccaccaat
    55861 catccattgg ctgtttttta cgaatagatc ctttctcttg tatatatcct ccagttcagg
    55921 gacaattgat cgttcgcttt cgatcgcagc ttgcagtttt gctgctcttt gctgcgttcc
    55981 ttcgctctca aacatgtgta ccaaccagtc ttccatcaat tctcgtagtt cttctgatac
    56041 ttttggcaat acttctttga ttgaacttgc caaagcctcg cgacgagtct gtgccgctag
    56101 aagcatacca taaccaaatt cagcattatc ttccaataaa gaatttgacc atgcaggccc
    56161 ttgaccacat tcattggtcg tataaggaga agcaggagaa gcaccgcccc aaatagaaga
    56221 acagcctgtc gcattcgcga tcatcattcg atcaccgaac atttgtgtca ataattttac
    56281 ataaggtgtt tcgccacagc ctgagcaagc gcccgagaat tcaagaagcg gttgattgaa
    56341 ttgtgtagac aagacagtat tctttccttt gactggattt gctttttgtc ttaatgtcat
    56401 tgcaaaagcc caattcacag cttgttcttt ctgttcttca taaggcttca tcactaaagc
    56461 tttttctttt gcgggacaag cctctacaca cagaccacaa cctgtacagt cttccactga
    56521 aacttggata cggtatttta cgccgtctgc gcctcgcatt tctctcacga taaatccttc
    56581 tggcgcttct tccatttcct cttcatctgc taaaaatgga cggatggcag catgaggaca
    56641 aacaaaggca cattcattac acatcgtaca gcgatcacta atccactcag gtacttctaa
    56701 agcgacgccc cgcttttcca tagaagccgt tcctaaaggc atttcgccag ttgtcattcc
    56761 atttgctaca agtgtaccaa cagagagttg atttccttcc tgtgcattca ctggttcaag
    56821 tatttcttgt atataaccag tgttcctttt attgattttt tcaggcatct ccaacgttct
    56881 ccaatggtct gggatcgcga cttgatgaag cagttcggtc gtccgatcga ttgcttgtat
    56941 gtttttttct acgatagcca ttgatttatg tccatagctt ttcagggctt cttcttttag
    57001 cagcggaagc acctcatcaa aaggaatgat gtcagctaat ttaaaaaaag ccgtttccat
    57061 tgccgtattg atccggcgtc ccaaaccgac ttcttgggcc aatctcatgg cattgatcgt
    57121 atagaatcta atgttatttt cagccaagta acgttttaat tttaagggca gatgcgtttc
    57181 taattgttcg tcgctccaaa gagtattcaa taaaaaaatg ccacctggct tcaatccttt
    57241 gaccaagtca tagctatgca ggtaagctgg tgtgtgacag gcaacaaggt ccgcatgctc
    57301 gacaagatac gctgaacgta tcggcgtatc tccaaaacga agatgagaga cagtcagacc
    57361 gccagatttt ttcgaatcat aataaaaata gccttgcgca tatttatcgg tgtgatctcc
    57421 aataatcttg atagctgatt tatttgctcc aactgttccg tcagatccaa atccccaaaa
    57481 cttggcctgg aatgtttgcg gttctgtcaa atccagactt tcctttggtt ccaaagattg
    57541 ataagtcaca tcatccacga tccctatggt aaaccgtttt ttgcgtatgc ttgggtcttt
    57601 tttcaattca tcaaaaactg ctgagatttg gtcgggagtc acatctttag accccaatcc
    57661 gtagcgacca ccaataacag ccgggcgaag ttcagaatca tataacgcac tttgaacgtc
    57721 taataagaga ggttctccac ctgcccctgg ttctttactt cgatcaagaa cagcaattgt
    57781 ttttactgta tgaggcaact tttgcacgaa tgtttccact ggaaaaggac gataaagatg
    57841 gatattcaaa aaacctgttt tccgtccgtt tgcattcaag taatcaatcg tttgttcaat
    57901 cgtctgtgca actgatccca tagaaacaat gacttcttcc gcatcgtctg ctccgtaata
    57961 agtcaccaag tcataagaag ttcctcgtaa tcggttgatc tctttcatat atttttgaac
    58021 gatttcaggt aatttatcat aataggggtt gatcgtttcc cgttgttgaa aatggatgtc
    58081 tggattctgg ttcgtaccac taacagtcgg atgattggga ttcatccctc gtttacgaaa
    58141 tgaagctaat ttctcctgat tgatcagagg agctaattct tcatattcca aaacttcgat
    58201 tttttgaatc tcgtgactcg tacggaatcc atcaaagaaa ttcaaaaaag gtacttctcc
    58261 ttcaatagaa gcaagatggg caacaggtgc taaatccatc acttcttgta cactgctttc
    58321 agctaacata gcaaatcctg tttgtcttgc agccatcacg tctccgtgat ctccgaaaat
    58381 attcaacgca tttgtcgtca ctgcccgact agcaacatgg aaaacacttg gcagtagttc
    58441 cccagcaatc ttgtacatat tcggtatcat caaaagcagc ccttgagaag cggtatatgt
    58501 agttgtcagt gcaccagttt tcaatgatcc atggaccaca ccagctgctc ctgcttcgga
    58561 ttgcatttct actactttta ctggttgacc gaatacattt tcttttcctg ctgctgacca
    58621 ttgatctact aattcagcca tggtcgaact gggtgttatt ggatatattg cagcaatctc
    58681 ggtaaatgca taagaaatat atgcggctgc cgtattgcca tccattgttt tcatttttcg
    58741 catgattcat catccttgtt catattctct cttattatct gttccattat aacgtaaaac
    58801 attccatttt ttcttttatt tataaaaata tttcacatat gtctatttga attggtcatc
    58861 taatttttag acaaacagtc taaatcgttg cctttttttg tcagacatgt ataaactata
    58921 agcgaaagag gtgttttaac atggatatat cagtgattga tgcaacgaaa acaaatacac
    58981 aaaaaggaat cctttatggt tcatcgaatg ctcctaaaaa aatggtcgaa ttcatcaacc
    59041 tatcttgtcc gtattgccga caatggtttg aagaatctta tgagctatta gaagaagcag
    59101 tccaaagcgg gcaattgcag cgagtgatca aattgttcga taaagaaaaa gaaagcttgc
    59161 agcgaggaaa cgtgatgcac cggtatctca caatcagcga tggtcaaaaa gcgatcaaag
    59221 agatcaaaca aatattcgat actcaagacg agtggaaaca tctttctctc caagaagtag
    59281 cggattttgc agtagataaa ctaaagctga cagaacaaaa agacgaacag ctgagccaag
    59341 ctgtcatcaa cgaagctgag caagcacata ttcgatttgt tccgactgtc atcttgggta
    59401 aagaaatctt tgatgaatct atttcaatag aggaattaaa agaattgatc caaacaaaat
    59461 aaaaaaataa ggagcgtagg acaaaagtaa agaacacttt tgtctcacgc tctaaacacc
    59521 tataaacggc ggaagcagaa gcaactcctt cttatttctt ggagttaaac gcttctgtcc
    59581 cagcctcctt attccgaata aatggtgtcc aaaagcttgc atctgccaga ttatctcatt
    59641 tcttatgaca agctgagcaa attatctcca ccaaataatt tattcatatt tttgacttgc
    59701 gcatactaac taattcttaa attttcatac aaaatttctc aaattccctt aacgagtaat
    59761 atttatgtac ggtgattcac actagtatcc caatcacttt ttaagttatc ccgatttcaa
    59821 ttctacttca aaaatagttg cttttatgta tgctgttagt tgattagatg gtcgtttgtt
    59881 gagctaggtc gtttatttta aacaagttgt ttctttagct tatacctatc tcgtctacca
    59941 aacgcaagat ggcaaacaca aatgcatacg catctgtatt tgccactttg attcgtataa
    60001 agctaacaac tcctaactcc tctcatttaa tagttgctct cttttttatt cagatacatt
    60061 tttgctgatc acagcctagt atttcaatgg ttttgaacac taaaccaaat tgttccatta
    60121 caacattcac tggctgatga aatggattaa tagatttcga ttattcaaag cgattttcta
    60181 ttggtggatt gtgttcccaa ctaacaagac ggattccaaa gtcattatat aactcttcaa
    60241 gagttaaccc tttcctgttc ccaatagatg tatctctccc acgctcgata taaaatatgg
    60301 agtttcgaat gccaagacct ggcaaatctt tccttgttac agttacactg ctactaactt
    60361 ccccatataa aatccagctc ctccaaaatg ataatatatt tttcgaccat catctaactc
    60421 atgtaacaat aattgttgtg ccctcttgtc actcgccaaa tgcaaacgcc acgtgcgata
    60481 ccatgccatg ctacctgcat ctgcagaaaa tccatcaaat aatgcaacaa ttgtatcttc
    60541 tatcacaatc ctttccccat ctacttcata ttctaatcta aaatcaaact cccctcgggt
    60601 aatcgcaggt cttggtggct taggacccaa tattattcta cttaaaaccg cattcgttcc
    60661 cagatgaaac acagctataa caacaataat gattaatatt ttgtttctat ttgataaatt
    60721 tctcattatt cgcaaatcac ctctacattc tcacatacta gttcgtaata ctcctttaat
    60781 atcaaaatat ctactgatcg taataatatt cgctatgaat tcctactact cgtataagaa
    60841 aggccaaatc atcagctttt acttgctgat gatttggctt gtttctattt atcgtaattg
    60901 ttttctaatc accaaaattg ttaacaatcg gttcagatat ttcccaattc actagcctta
    60961 tattatattg atttaacaat tcttcttcat ttattgtatt ttgatcaagc ggaccaaatt
    61021 gttctaataa ttctttgtca tgtaaaatat cccttctagt aatgcctctt tccctttcaa
    61081 tgaaagaagc actaggaaaa gtatgattat aaccattcaa cctatctccc atgtaataat
    61141 tagcaggtcc tggaaaataa aatatttgct tattatcact aacttctaac agcaacacta
    61201 aatcttctcc gctggctaaa cgacttgtcc atctacgata tctccctatg ccttcgttca
    61261 ttccaaatcc atcaaattca actattaacg tgtcttctat cacatgtaat tcttcattca
    61321 attcatactc aagtctaaaa ggaaattctc catatgtaat ctctggcaat ggtggtgctg
    61381 gagttctcca aataaataca ataactgtga atagaaaaaa tccaacgatc gatataaatg
    61441 ttatgagacc tgctcgaagt aaaacccgat ataataaaaa tgctgtattt cgctttgcta
    61501 tatctagaaa aataaaaagt actgcaatca taatgatagt aaaagttgta aaaaaacgca
    61561 tttttgccct ccctttataa acatcgattc tcaaattcta aaccaaatct tggtagttca
    61621 agttcaggta taacgtattc caaccataca tctcaattac tatttatgaa cttatgatta
    61681 tcactgaata aatcactcca tccccttgtt aaaacttcta cattaattac acctggttgt
    61741 tttcgactag ctaatccaat gaacttatca accatcctta tccaccaacc acgagaatct
    61801 tgaccagaag taagaagagc agttctcaag gatgatcgag cagttccttc cagtttttta
    61861 attaatcagg ccagttatag ataaatactt gatcttatct atatcaggac ttctatatta
    61921 agcaaaaatt tatgtacaaa actaaaatct tcattcagtt gtctccccgc tttttatacg
    61981 attgtattaa aaacaataga agcctttcca aattatgtta ctccagtaaa gatggaatta
    62041 tagtttgacg gaagaaaagc aagtataact tttaaaaaaa actgatacat tcgcatctcc
    62101 tgcttcatgt atcagttttt tctttggatt aaactgcttc ttcacgcagt ttttccgcaa
    62161 attcatttat tttgcgaatt cggtgattaa ttcccgattt tgatatcgca cctgagggaa
    62221 tcatttcccc taattctttc aagctgacct ctggatgttg aagccgaagt tctgcgattt
    62281 cctgaagttt ttcaggaagg gcatgtagac cgacctttgc ttcgatcaat tcgatgtttt
    62341 cgatttgttt agaagccgca tcaatcgtct tgttcagatt tgctgtctca caattgacta
    62401 gacgattgac cgaatttctc atatcccgta cgattcgaac atcttcaaat cggagcattg
    62461 agttggttgc accaatcaat gttaagaaat cagcaatttt ttctgctcct tttaaataag
    62521 aaatatagcc attgcgccgt tcaagcgttc gagcatgtag accgtaataa tttaacatat
    62581 cgcaaatgtc ttggttatgc tgttcataaa tagaaaatat ttccaaatga taacggctcg
    62641 tttccggatt attgactgaa cctgaggcca taaaagcacc acgtaagtaa gaacgcattt
    62701 tttgcgaatt tcccataatc tcgtccgaaa cgtgtccatt gaacatcata ccatccatga
    62761 tatcaagatc catcaacaca tcttttgtgc cttgcttcaa ccggacgata tagacattgt
    62821 tcttttttaa tttcattttt ttacgaacga gtagctcact tcgtacctga taatgatctt
    62881 tcaaaagaga atagatacgt ctggcaattg cagcattttc tgtttggacg ttcaaaataa
    62941 actgctggtt cacaatacct aaagagccgt tcattcgcag caaagcagct aattccgctt
    63001 tcgcatgttc tcgatgaact tccaaaccag ttagttcttt cttcacatct gcagcaaatg
    63061 acatcctatt ctcctccttt ctccaacaat aaattgctta atattttgaa ccgaaaacta
    63121 aacggaacaa ttcttctact actttgtctc catcgtggaa gacaccacca tctttcaatt
    63181 ccaaaaagtc ggtggaaatc actcgacatc cttcatttct caatccgttg aagtcgtgtg
    63241 cgacttgtac tagatactca tcatagatct ctgggtccat ataatcttca ggcacttttt
    63301 ccgtattgac caatactgta tcgataaaag gctgattcaa atgcttgtgt aaaacacgta
    63361 tatgatcggc atctgtgaaa tgttccgttt cccctttttg ggtcatgata ttacagatat
    63421 agacggtttc agcagttgtc ttgagcattg cttcgccgat ttctggtatc actaaatttg
    63481 gaaggatact ggtgaataaa cttccgggtc ccaaaacgac catgtcggcg tccataattg
    63541 ctgaaacgac ttttcgagcc gctttggctt cacttttatc atttgtgttg cagacataca
    63601 cacggtcaat ggttttacgg tcgatcgcga ttttagattc tcctactgca gtcgttccgt
    63661 ctttaaagac agcatgcaag gtcaaaggag tttctgaaga aggataaaca tgtccatcta
    63721 catgcatcat ttttgctaat aattgaatcg cctcataagt gcttccccgc atctcggaaa
    63781 ctgcggcaat gatcagatta ccgatcgtat gattggctaa aaactgatct tcctttttga
    63841 aacggtattg gaaaatatct tcatagaatt gtggcatatc cgataacgcc actaagacat
    63901 ttctcaagtc accaggagga gccatagcga tcgattctct gattgcaccg ctgcttcccc
    63961 catcatctgc aacagtcact actgctgtga tatctgctcc ttgatttcgc aaacttttca
    64021 ggataactgg cagaccagtg ccaccgccga ccactacgat ctttggtttt cttatccggt
    64081 aggttttcat tttcacgacc ggttcaccgt ctctttcctt ttatccttgt cccgatgtgt
    64141 cacattgact ttatagtttt tccctaaagc atttgccaaa cgttcagtca atgccacaga
    64201 acggtgttga ccgcctgtac agccgattgc aactgtcaag ctgctttttc cttctttaat
    64261 gtatccaggc aatacagttt ccagaagatc caaaaattta tggtagaatg attgcgtttc
    64321 atcaaaggac atcacataat catagactgg ctgatctttt ccggtcaatg gtcttagttc
    64381 cgggatataa tgagggttgg gcaaaaaacg cacatccatc acaatatccg aatcgatcgg
    64441 caacccgtat ttaaaaccaa atgatacaaa ttcgatccga aatccttggt ctccctttga
    64501 tttgaatgac tcattgattt tttcacgtaa ttgtcgtggt gtcaatgtcg tcgtatcaat
    64561 gatcacggaa gcttgggctt tcaagtcgtc tagtatcgcc cgttctttac gtatcccttc
    64621 agtgatcaaa ccgtccatcg ccattggatg tgctcgtctt gtttctttgt agcgagccac
    64681 tagttcttcg tctgaagcat ctaaaaataa gatagaagtg tcgatgaagt tcgtattttc
    64741 gatttcgatc agcatgtttt ggatttcttc aaagaaggaa cgagaacgta aatccactcc
    64801 taaagccatt tttgtgactt tgcctgattc tttgattaat tcccagaatt ttggaatcaa
    64861 tcgaggcggc atattatcga tacaaaaata gcccatatct tcaaaacttt ggatagctac
    64921 tgttttccct gcaccgctca tacctgttat gatgactaat tgcaaattat ctgtcataat
    64981 tacgaactcc tctcacattg tgcatagtaa aattatatca ttccgggcgt atcattacca
    65041 gttttctttt tcttttacaa aaagatttgg accaaatatc ttttaggata tttgccccaa
    65101 atccaatttt tttacttttc cgatgctgta tccaatgcct tgcttctttc agctagcgct
    65161 cgttcaatcg tccgatctgc taaaatagat tgggcatcga tcactggata atggttatct
    65221 tcagcaaatt catgcatcaa tgacaattct gtgcaaccta agataacttt ttcacagttt
    65281 aggcgctcta ccgcttcttc taagatttcg taatatagct cttgattcaa ataatccgat
    65341 tccttgatct catgatagat cagataattg attttttctt gaagaacagt atcaggaatc
    65401 actgtttcga atccaagatt tttgacttcg cgttcataga ttccagcttt catacttcct
    65461 tctgttccta gaatcgctac tctccctgtc gtatgctgac ggacaagttc atttgctgct
    65521 tctcttggca tatgaaggat aggaatatcc gttgccgctt gtaattcttc aaaaaagtag
    65581 tgtgcggtgt tacaagtcaa cacaatgaag ttcggctgca ataaattttg tttctcaata
    65641 tcatccagca gaaacggcat tggattttct tctgatcgat ctaaaatata agctgtacga
    65701 tcaggaactg ttgcatgatt gaataagaca taattcaaat attcttgatc ttttgtagct
    65761 ttcgttcggt ggttgatcaa gcgaacaaaa ctttctgtcg ccatcgtgcc cattccgcct
    65821 aaaatactga aaaaattctc catgaactag ccttctttca catgaaaata ctttttgaat
    65881 cttggaatat agttatggaa catatatttc atcaacaacc agcgcttgat tgaccggtct
    65941 ttttcataaa acacagtagt accgtaacga ttttctttga tcatttgctc agctagtttt
    66001 ttgtcttcat tttcccgagc atattctaaa aagatttttt tgggcacacc catccataat
    66061 cttgctttat ctgattgatt cgttccatag gtcgtttcaa caaagggttt gttaaacact
    66121 cgatcttctg tgacgaaacg agccagattc aagccgttca atgtcacaaa gaaactgcta
    66181 cgaccttgtc tcaaattgat ttcaaataat ttatattctc catctctagg atcatacttc
    66241 atatcgaaat tggcaaagcc tgtatattcg attttttcca aaaatgcctt aatggtttga
    66301 tagatttttt cgttataatc tggcatgatc acaacgtaat taccaatcga tgctggtgta
    66361 ggatcttcta acagtggatg accaagacac atcatccgaa cttgatgatc ttcatctaca
    66421 tacgcattta gcacacgcat attactatca tcacccggga taaaatcttg tacaatcatt
    66481 tcacttttat aaccggcttc gtatatccgg cctaaaatta aatcgaactc ttctctagtc
    66541 tctaaaataa aagctttttt tcttccttca aattggacag aaagatactc cacactgttt
    66601 gcaggtttca aagcaactgg gaattcaaaa ggcaattcct gttccaaatg tccatttact
    66661 aacatttctt ctctgacgat caatgtttta ggatacggaa gatcatattc ttcacaaact
    66721 tcataaaagc ttactttatt gatcagtctt tcaaataaag aataatcaat atatggacag
    66781 ataaaatatt ctgacagctc ttgtttatgt tgggagatca attctgcata tccatcacca
    66841 caagcaatca aaagatattt tttactctta tcagaatatc tttcttttgc caaacgaagc
    66901 atcgtctcga taaacacagg gtctttatca aatcctggaa taacttctac attgacaatt
    66961 ttactgtagc gggtaggggc taactgatcc gaagcatatg cttggcagat tttgccgtat
    67021 gcttcattaa aagaacgtgc cataccataa acgttcatgt cgcttcctaa taatataggg
    67081 ataaattctt cattttcaat actgttcatc attatctctc acttactcat aatctcttgt
    67141 ttttcttttc caactacaca aggaatatca taccataaag aaaaaagcaa tcaaaggaaa
    67201 ctcctccgat tgctcaattc ttttttgaac ggattgaact tataggaagt ttttgtctat
    67261 accaatcagt gtagaaagaa ctaccatgta atgattctag aacactgata ttaataaaac
    67321 aagcaaaaat tcattttttt atgttttttt aactatctca gctatctatc ctctttatac
    67381 cattattttt ttcaaatagt gacctgtata actttctttt acgttaatga tttcttctgg
    67441 tgttccagtc gcaaggatcg ttcctccacc ttctccacct tctggtccta aatcaatgat
    67501 ataatctgct gttttgatta catctagatt gtgttcaatc actaaaactg tgttaccagc
    67561 atctactaat ctttgtaata catgcaacaa gcgggcgatg tcatcgctat gaagtcctgt
    67621 cgttggttca tctagtatat agaaattctt tccattagag attttgtgca attcactggc
    67681 aagtttcatc cgttgtgcct caccaccgga caatgtcgtt gctggttgcc ccatcgtcac
    67741 ataacctaag ccaacatcaa caatcgtttg cagtttgcga tgaatctttg gaatgtgctt
    67801 gaagaattct acagcatctt ctactgtcat ttccaaaata tcagcaatgc tttttccttt
    67861 gtaatgcact tctaatgttt cagagttata tcgtttgcca tgacatactt cacaaggaac
    67921 atagacatca ggcaaaaagt gcatttcgat cttaataatt ccatccccgc gacaagcttc
    67981 acaacgaccg ccttttacgt tgaaactaaa acgccctttc ttataacccc gcatttttgc
    68041 ctcgttcgtt tgagcaaata acccacggat atcatcaaat acacttgtat aagtcgctgg
    68101 attacttctc ggcgtccggc cgattgggct ttgatcgata tcgatgatct tttcgatact
    68161 ttcgtagccg gaaattgtct tgaatttacc tggcttagca gaattcttat ttaatttttg
    68221 cgctaacgat ttctttaaga tactattgac caatgtactc tttccagaac cagacacacc
    68281 tgtgactgct acaaattctc ctaatggaaa ttcaacacta atatttttca gattgttttc
    68341 tgaagcacca gtgatcttga ttgctttccc attaccttta cggcgttctt ttggtacagg
    68401 gattgatttt ttccctgaaa gatatttccc tgtcaaagaa ttttcatcat tagctacttc
    68461 ttcaggagtg cctgcagcca cgatttgtcc gccttgttct ccggccccag gtccgacgtc
    68521 aatcagataa tctgctgccc acatcgtatc ttcatcatgt tcgactacaa cgagggtgtt
    68581 gccaagatca cgcattttct ttaatgagcc aatcagtcga tcgttgtcgc gttgatgcaa
    68641 tccgatcgat ggttcatcta aaatatacaa aactccagaa agattcgatc caatttgtgt
    68701 agccaatctg attcgctgcg cttctccccc agataatgtt ccggaagcgc gactcaaagt
    68761 tagatagtct agtcccacat tttccaagaa tgaaagtctg tcatttactt cttttaaaat
    68821 cggtttggca attactttct cttgttcaga taacgttaag tttgagaaga aatctactgc
    68881 tttttgaata gacaactcat ttgtttgccc gatattcgtt ccattgattt gaacagatag
    68941 agcttgggga ttcaatcgat aacctttaca tgtctgacaa gttaattctg tcatataaag
    69001 acgcatttgc tcacgcgtga aatcactgtt cgtatcatga taacgacgct cgatattctt
    69061 caatacccct tcgaaagtcg tttctacatc gcgaacgcca ccaaaatcat tttcataatg
    69121 gaaatggaaa agatcatctg tcccattcaa gatgatatcc tgatgctctt ccggtaattc
    69181 ttcgaatggt gtatctaaat cgataccgaa actttcacaa gcctgcgcta acatttgagg
    69241 ataatactga gagctgattg gattccatgg aacgatagca ccttgactaa gtgtcttcgt
    69301 tctatctgga atcactaaat cttgatcgac ttctaatttg acacctaaac catcacagtc
    69361 tggacaagct ccaaaaggcg cattgaatga gaacagtctt ggttctagtt cgccaacagt
    69421 aaatccgcaa taaggacagg catagtgttc gctaaacaac atttcttctt caccgatcac
    69481 gtcaaccaga gcatagccgt ctgccaatcg cagagccgcc tctaaagaat caaatagacg
    69541 agaacgaatc ccttctttca caatgatccg gtcgatgacg atcgcgatat catgtttttt
    69601 gtttttctcc agctcaggca cttcacttaa atcatagatc tcaccatcga ctcgaacgcg
    69661 gacgtatccc tctttttgga ttcgttcgaa tattttttta tgttgtcctt ttttctttac
    69721 cacgatcggt gccaacactt ggatctttgt gcgctcaggc aaagccatta cttgatctgc
    69781 catttgatct actgattgac tggtaatctc gatatgatcg ttgggacaaa tcggatgtcc
    69841 tacacgtgca aacaacaagc gtaaatagtc attgatttcc gtcacagtac ctactgtcga
    69901 ccgaggattt ttacttgttg ttttctgatc aatagaaatc gccggactca atccgtcgat
    69961 gctatctacg tccggcttat ccatttgtcc taaaaactga cgagcataag cagaaaggct
    70021 ctctacgtat ctccgctgcc cttcagcgta cagcgtatca aatgctaggg aactttttcc
    70081 agaaccggac aaacctgtaa cgaccacgaa tttatctcgt ggaatcgtta catctatatt
    70141 tttcaagtta tgggcacgtg caccgtgaat cgtaatttta tcgttagcca tgttgtcctc
    70201 ctactttgaa gcttttaatt ctaaaatcat atctcgcaca ttagcggctg tttcaaaatc
    70261 caatgctttc gccgcatttt tcatttcttt ttctaatttc atcaataacg tatctttttc
    70321 ttctttcgtc atttcttcat acgagacttg taccgcttct tttgtatcat cttccgattc
    70381 tttcgtaata gaaatcaaat ctcggatttc tttaatgatc gtttttggaa cgatcccatg
    70441 ttcttcattg tacttctgct gaatcgtacg gcgtcttgat gtttcatcca tcgctagacg
    70501 catagaatct gttactttgt ctgcgtacat gattacttta ccttcttcat tccgagcagc
    70561 tcgtccaatc gtttggacta aggaacgttc actacgtaaa aagccttctt tatccgcatc
    70621 caagatagca acaagggaaa cttcaggtac gtctaatcct tcacgaagca aattgattcc
    70681 aactaacaca tcaaattcac ctaatcgaag atctcgaatg atttctgttc gttccagcgt
    70741 tttgatatca ctatgcaaat atttgacttt gatccctaat tctttgaaat aatctgtgag
    70801 gtcttctgcc atttttttcg tcaatgtcgt tacaaacacc cgctggtctt tttcgactcg
    70861 ttcatttatt tcgcctacta gatcatcgat ttgtcccatg attggacgaa cttcgatcac
    70921 tgggtcaagc agccctgtcg gacggatgat ttgttggata acggtatctg tttgttcatg
    70981 ttcatatggt cctggcgtcg cagagacata aatgatctga ttgacatgtt tttcaaactc
    71041 ttctaaccga agtggccgat tgtctaaagc agatggcaaa cggaaaccgt aatctactag
    71101 catctgtttt ctcgctcggt caccattata cattcctctg acttgaggca tggttacgtg
    71161 cgattcgtcc gccacgatca aaaaatcttc aggaaagaaa tccaatagcg tataaggcgg
    71221 ttcaccttct ttacgtccgt ccatatgccg agaataattt tcaattcccg acgtgtagcc
    71281 catttctaac atcatttcaa tatcgtaatt ggttcgctgt tctaaacgtt gagcttccag
    71341 aagtttgttt tcatttcgca agacagttaa acgctgttct aattccgctt tgatattcgc
    71401 tacggcatgc tccatatgtt catcattcgt cacaaagtga gtcgccggga agattgccac
    71461 gtgttccgtt tctcctaaaa cttcacctgt cagtgcattg acttctcgga tacgttcgac
    71521 ctcatctcca aaaaactcca cacgtaaggc acgttcatct cttgaagccg gaaaaatctc
    71581 cacgacatca ccgcgcactc gaaaacgacc gcgctgaaaa tctatatcat ttctttcgaa
    71641 ttggatactg actaagtcac gaatcaactg attgcgatct agttctgctc cctgtcgaat
    71701 cgaaactact tgtttttggt actcaaaagg cgaacctaat ccaaaaatac atgaaacgga
    71761 tgcaatgacg atcacatcat ttctttctaa taatgaacta gtagccgaat gtcgtaattt
    71821 gtcgatctca tcatttacac ttgaatcttt ttcgatatac gtatcgcttg aaggcacata
    71881 agcttcaggc tgataataat cataataaga aacaaaatat tcaacagcat tgttagggaa
    71941 aaattccttg aattccccgt acagctgacc agccaatgtt ttgttatggg caataattag
    72001 tgttggttta ttcactttct caataagatt ggaaatcgta tatgttttcc ctgttccagt
    72061 cgcacctaat aagatttgtg cttttttccc gccgactacg ccatccacta attggttaat
    72121 cgcttcaggc tgatcaccag ccggttggta tttcgagaca agctcaaagt gtcgggaggt
    72181 atctctctca atcattcttc gtcctccttt tcattcattc cttttcattt tacgaccaat
    72241 aaaaagaaaa ggaaagcaaa ggcattcctt actaaaaaat tataccacac cgaacatact
    72301 ttcgccactt ttttatctta ttatgcatgt tacgttttat aacataaaat gttagaaact
    72361 cgcatttttt tcattttttc gagcttacag tcttgtctga ttcagcactt ttcgcctata
    72421 ataataaaat attggaaatt taggaggttg attatgaaaa gaaagcattt atttcactca
    72481 tttgttttct taatgacagg acttgtgaca cttgcgtttg gacatgttgc acaagctgac
    72541 aacactacaa cggcagagtc aagcacaagc gagacaatgc aaaaaataga gcccaaaaaa
    72601 gacgtatacg taattgccag tgactctgct tttgctccat ttgaatttca aaacgcaaag
    72661 ggcgagtacg aaggcatcga tgtaaaattg atggaaaaca tcgccaaact gcaaggattt
    72721 aacatccgaa tggactaccg tggtttctct gctgctttgc aagctttgga atcaggacaa
    72781 gcagacggaa tgattgccgg aatgactgta acagatgaac gaaagaaaag ctatgatttt
    72841 tctgattctt actttgatag cgggatccaa ttagctgtca aaaaaggaga tacttcgatc
    72901 aagtcttatg aagatttgaa aggaaaaaca gtcggagcaa aagttggtac cgaaagtgct
    72961 gaatttttaa gcgaccatga atctgaatat ggctattcta tcaaatattt tgatgctgct
    73021 gatcaattgt acgacgggtt aaaagttggc gcaatcgatg caatgatgga tgattatccg
    73081 gtaatcggtt atgcgatcaa acaaggacaa gaattggaaa caccgatcaa aaaagaatct
    73141 ggcggaaaat atgcatttgc cgttaaaaaa ggacaaaatc ctgagcttct taatatgttc
    73201 aatgaaggat tgaaagaatt aaaaagaaca ggcgaatacg ataagattat taaagaatat
    73261 gtagcggatg gttcagaaac ttccagtgag tcttccactg ctgatgaatc aacatttctt
    73321 ggacttatcc aaaacaacta tcaagcattg ttgaaaggac tttggagaac aattctccta
    73381 actttgatct cctttgcttt ggctatagta atcggtgtga tttttggatt attcagtgta
    73441 gcaccaatac gcgctcttcg cacaattgct agtatttatg tcgatatcat ccgaggaatt
    73501 ccaatgatgg ttcttgcgtt ctttattttc tttgggttgc ctggtatcgt tggatttacg
    73561 attcctgact tccttgctgg tgttatcacc ttgactctta acgcaagtgc ttatatcgca
    73621 gagattgttc gaggcgggat caacgccgtt cctgtcggac aaatggaagc ttccagaagt
    73681 ttaggtcttt cttataatcg gacaatgcaa aaaatcattt tgccgcaagc gatacgcatc
    73741 atgatcccat cattcgtcaa tcagttcgtt atctcattaa aagacacaac gatcatttcc
    73801 gcaatcggtg tagtcgaatt gctgcaaact ggtaagatca ttgttgcacg tacaacacaa
    73861 agtacgtatg tttacttgat cattgcgatc atgtacttga ttttgatcac agcattgaca
    73921 aaattagcaa aagttttaga aaagaaggtg aagtaagatg gcagaaaaaa tcttagttga
    73981 gcatttagtc aaaaaatacg gcgataacac agtattaaac gacatcaatg tgtccatcaa
    74041 cgaaggagat gtcgtctgta tcattggccc ttcaggttca ggaaaaagta catttttgcg
    74101 ttgcttgaat cgtttagaag aaccttctga cggtgaaatt attattgacg gcgcccactt
    74161 gatggataaa agcacagata tcaacttggt gagacaacat atcggaatgg tattccagca
    74221 ctttaaccta ttccctcatc tttctatttt ggaaaacatc attttagcac cgatggacct
    74281 gaagaaagaa tctagagaag aagcagagaa aaaagcaatc aaattgctgc agacagttgg
    74341 tctagaagat aaaaaagata tgtatcctga aatgctttcc ggtggacaaa agcagcgtgt
    74401 ggctattgct cgtgctctag cgatgaaccc tgacatcatg ctttttgatg agcctacatc
    74461 tgcactcgac cccgaaatgg tcggtgatgt cttgaacgtc atgaaaaagt tagcagaaca
    74521 aggtatgacg atggttatcg ttacgcacga aatgggcttc gctaaagaag tggccaatcg
    74581 tgtcatgttc attgacgacg gaaacttttt agaagacgga acgcctcaac aaatctttga
    74641 aaatccgcaa aatgaacgga caaaagat
//
LOCUS       DACECZ010000004        61301 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.004, whole genome shotgun sequence.
ACCESSION   DACECZ010000004 DACECZ010000000
VERSION     DACECZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.004"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            200..1579
                     /locus_tag="IWO35_000290"
     CDS             200..1579
                     /locus_tag="IWO35_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0315375.1"
                     /translation="MMGNLVLSYNIYYFFITFLTMVVSYFSMKQIKNNRYISLLFSII
                     YTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTH
                     LLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQE
                     LKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFF
                     LGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNS
                     WKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYANKESIYHP
                     DMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASINGKL
                     VQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQ
                     PRREVQHSH"
     gene            complement(1724..2464)
                     /gene="truA"
                     /locus_tag="IWO35_000291"
     CDS             complement(1724..2464)
                     /gene="truA"
                     /locus_tag="IWO35_000291"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0315376.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2655..3290)
                     /locus_tag="IWO35_000292"
     CDS             complement(2655..3290)
                     /locus_tag="IWO35_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ0315377.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3437..4678)
                     /gene="sstT"
                     /locus_tag="IWO35_000293"
     CDS             complement(3437..4678)
                     /gene="sstT"
                     /locus_tag="IWO35_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ0315378.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4876..5874
                     /locus_tag="IWO35_000294"
     CDS             4876..5874
                     /locus_tag="IWO35_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315379.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            5887..7905
                     /locus_tag="IWO35_000295"
     CDS             5887..7905
                     /locus_tag="IWO35_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAQ0315380.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(7965..8681)
                     /locus_tag="IWO35_000296"
     CDS             complement(7965..8681)
                     /locus_tag="IWO35_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ0315381.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8908..9450)
                     /locus_tag="IWO35_000297"
     CDS             complement(8908..9450)
                     /locus_tag="IWO35_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0315382.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9455..9682)
                     /locus_tag="IWO35_000298"
     CDS             complement(9455..9682)
                     /locus_tag="IWO35_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAQ0315383.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9761..11641)
                     /locus_tag="IWO35_000299"
     CDS             complement(9761..11641)
                     /locus_tag="IWO35_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ0315384.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11830..12444)
                     /locus_tag="IWO35_000300"
     CDS             complement(11830..12444)
                     /locus_tag="IWO35_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0315385.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12586..13146
                     /locus_tag="IWO35_000301"
     CDS             12586..13146
                     /locus_tag="IWO35_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0315386.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13451..13765)
                     /locus_tag="IWO35_000302"
                     /pseudo
     CDS             complement(13451..13765)
                     /locus_tag="IWO35_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(14019..15654)
                     /locus_tag="IWO35_000303"
                     /pseudo
     CDS             complement(14019..15654)
                     /locus_tag="IWO35_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15977..16456)
                     /gene="rlmH"
                     /locus_tag="IWO35_000304"
     CDS             complement(15977..16456)
                     /gene="rlmH"
                     /locus_tag="IWO35_000304"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ0315387.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            16884..18173
                     /locus_tag="IWO35_000305"
     CDS             16884..18173
                     /locus_tag="IWO35_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0315388.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(18212..18817)
                     /locus_tag="IWO35_000306"
     CDS             complement(18212..18817)
                     /locus_tag="IWO35_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ0315389.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(19022..19486)
                     /locus_tag="IWO35_000307"
     CDS             complement(19022..19486)
                     /locus_tag="IWO35_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0315390.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(19508..19828)
                     /locus_tag="IWO35_000308"
     CDS             complement(19508..19828)
                     /locus_tag="IWO35_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0315391.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(19972..21051)
                     /gene="pepA"
                     /locus_tag="IWO35_000309"
     CDS             complement(19972..21051)
                     /gene="pepA"
                     /locus_tag="IWO35_000309"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ0315392.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            21251..21601
                     /locus_tag="IWO35_000310"
     CDS             21251..21601
                     /locus_tag="IWO35_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0315393.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            21824..22771
                     /locus_tag="IWO35_000311"
     CDS             21824..22771
                     /locus_tag="IWO35_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0315394.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(22828..24246)
                     /gene="pepV"
                     /locus_tag="IWO35_000312"
     CDS             complement(22828..24246)
                     /gene="pepV"
                     /locus_tag="IWO35_000312"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ0315395.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            24395..25240
                     /locus_tag="IWO35_000313"
     CDS             24395..25240
                     /locus_tag="IWO35_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ0315396.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            25244..25684
                     /locus_tag="IWO35_000314"
     CDS             25244..25684
                     /locus_tag="IWO35_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ0315397.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            25734..25928
                     /locus_tag="IWO35_000315"
     CDS             25734..25928
                     /locus_tag="IWO35_000315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315398.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            26064..26750
                     /locus_tag="IWO35_000316"
     CDS             26064..26750
                     /locus_tag="IWO35_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0315399.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIRRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(27158..27847)
                     /locus_tag="IWO35_000317"
     CDS             complement(27158..27847)
                     /locus_tag="IWO35_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315400.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(27822..29951)
                     /locus_tag="IWO35_000318"
     CDS             complement(27822..29951)
                     /locus_tag="IWO35_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ0315401.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(29999..31336)
                     /locus_tag="IWO35_000319"
     CDS             complement(29999..31336)
                     /locus_tag="IWO35_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ0315402.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(31588..31947)
                     /gene="rplT"
                     /locus_tag="IWO35_000320"
     CDS             complement(31588..31947)
                     /gene="rplT"
                     /locus_tag="IWO35_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ0315403.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(32000..32200)
                     /gene="rpmI"
                     /locus_tag="IWO35_000321"
     CDS             complement(32000..32200)
                     /gene="rpmI"
                     /locus_tag="IWO35_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ0315404.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(32272..32796)
                     /gene="infC"
                     /locus_tag="IWO35_000322"
     CDS             complement(32272..32796)
                     /gene="infC"
                     /locus_tag="IWO35_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ0315405.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            33116..33748
                     /locus_tag="IWO35_000323"
     CDS             33116..33748
                     /locus_tag="IWO35_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0315406.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(33750..34655)
                     /locus_tag="IWO35_000324"
     CDS             complement(33750..34655)
                     /locus_tag="IWO35_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0315407.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(34740..35393)
                     /locus_tag="IWO35_000325"
     CDS             complement(34740..35393)
                     /locus_tag="IWO35_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0315408.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            35715..36659
                     /gene="mvk"
                     /locus_tag="IWO35_000326"
     CDS             35715..36659
                     /gene="mvk"
                     /locus_tag="IWO35_000326"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ0315409.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            36652..37629
                     /gene="mvaD"
                     /locus_tag="IWO35_000327"
     CDS             36652..37629
                     /gene="mvaD"
                     /locus_tag="IWO35_000327"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ0315410.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            37642..38727
                     /locus_tag="IWO35_000328"
     CDS             37642..38727
                     /locus_tag="IWO35_000328"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ0315411.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            38724..39788
                     /locus_tag="IWO35_000329"
     CDS             38724..39788
                     /locus_tag="IWO35_000329"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ0315412.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(40037..40840)
                     /locus_tag="IWO35_000330"
     CDS             complement(40037..40840)
                     /locus_tag="IWO35_000330"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ0315413.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDTKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(40860..41702)
                     /gene="kduI"
                     /locus_tag="IWO35_000331"
     CDS             complement(40860..41702)
                     /gene="kduI"
                     /locus_tag="IWO35_000331"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAQ0315414.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(41718..42725)
                     /locus_tag="IWO35_000332"
     CDS             complement(41718..42725)
                     /locus_tag="IWO35_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAQ0315415.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(42732..43376)
                     /locus_tag="IWO35_000333"
     CDS             complement(42732..43376)
                     /locus_tag="IWO35_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ0315416.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(43476..44234)
                     /locus_tag="IWO35_000334"
     CDS             complement(43476..44234)
                     /locus_tag="IWO35_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAQ0315417.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(44361..45512)
                     /gene="fucO"
                     /locus_tag="IWO35_000335"
     CDS             complement(44361..45512)
                     /gene="fucO"
                     /locus_tag="IWO35_000335"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAQ0315418.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(45594..45905)
                     /gene="rhaM"
                     /locus_tag="IWO35_000336"
     CDS             complement(45594..45905)
                     /gene="rhaM"
                     /locus_tag="IWO35_000336"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAQ0315419.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(45907..46752)
                     /gene="rhaD"
                     /locus_tag="IWO35_000337"
     CDS             complement(45907..46752)
                     /gene="rhaD"
                     /locus_tag="IWO35_000337"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAQ0315420.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(46766..48022)
                     /gene="rhaA"
                     /locus_tag="IWO35_000338"
     CDS             complement(46766..48022)
                     /gene="rhaA"
                     /locus_tag="IWO35_000338"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAQ0315421.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLEEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(48019..49479)
                     /gene="rhaB"
                     /locus_tag="IWO35_000339"
     CDS             complement(48019..49479)
                     /gene="rhaB"
                     /locus_tag="IWO35_000339"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAQ0315422.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            49632..50501
                     /locus_tag="IWO35_000340"
     CDS             49632..50501
                     /locus_tag="IWO35_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0315423.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(50627..51526)
                     /locus_tag="IWO35_000341"
     CDS             complement(50627..51526)
                     /locus_tag="IWO35_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0315424.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(51691..52515)
                     /locus_tag="IWO35_000342"
     CDS             complement(51691..52515)
                     /locus_tag="IWO35_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0315425.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(52526..53821)
                     /locus_tag="IWO35_000343"
     CDS             complement(52526..53821)
                     /locus_tag="IWO35_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAQ0315426.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(53837..54316)
                     /locus_tag="IWO35_000344"
     CDS             complement(53837..54316)
                     /locus_tag="IWO35_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ0315427.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(54322..55350)
                     /locus_tag="IWO35_000345"
     CDS             complement(54322..55350)
                     /locus_tag="IWO35_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAQ0315428.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(55367..56473)
                     /locus_tag="IWO35_000346"
     CDS             complement(55367..56473)
                     /locus_tag="IWO35_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0315429.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(56702..59299)
                     /gene="adhE"
                     /locus_tag="IWO35_000347"
                     /gene_synonym="adhC"
     CDS             complement(56702..59299)
                     /gene="adhE"
                     /locus_tag="IWO35_000347"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ0315430.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(59604..59984)
                     /gene="yajC"
                     /locus_tag="IWO35_000348"
     CDS             complement(59604..59984)
                     /gene="yajC"
                     /locus_tag="IWO35_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ0315431.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(60081..61226)
                     /gene="tgt"
                     /locus_tag="IWO35_000349"
     CDS             complement(60081..61226)
                     /gene="tgt"
                     /locus_tag="IWO35_000349"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ0315432.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
BASE COUNT        18020 a        12793 c        10761 g        19727 t
ORIGIN      
        1 ttacgtcatc cctctattca tgtctaaggg aatctatcat agtgtcaatc aggatactta
       61 ctttcattta tctcgaatca taggactaga taatgtatgg agttctccag taaacttcaa
      121 taacttcgat catcatggaa caatgatgaa tattttttat ccatggctta ctctttatcc
      181 agctttttta ttttataaaa tgatgggaaa tcttgtttta agttataata tctactattt
      241 ttttatcact tttttaacaa tggtcgtttc ttatttttct atgaaacaaa taaaaaacaa
      301 tcgttatatt tcgctgttat tttcgattat ttatacattt tccgcctatc gtgccattga
      361 catattccat cgtgcttcat tgggagaagc tgtcgcttta acatttttgc ctttgatact
      421 aatggggtgt tacgaaattt atattagaga ttaccaaaaa tggtactggt tgtctattgg
      481 catgacgcta gtagtgtata ctcatttact ttctgtcgct atggtttctg tatttattgg
      541 aggaacgttg tttctttctt tttatttttg ggaccaaaaa atcgctagac tcttaagtct
      601 tttaaaagct accgtcctca cctttttttt aagtgcggga ttcttaattc cttttattca
      661 acaatctaga gcacaagaat tgaaagtccc actgggaaaa gaattaagtg gtatggctcc
      721 tagtgatatg ctgacacata ttttaaataa taactataac aactatacta ttggattatt
      781 cttatttctc ggattgattg gtgcttttat ttttataaaa aaattaactg ctgatgattt
      841 atttattttc tttcttggtg tatttgttct tttttgttca accaatttat ttccttggca
      901 actttttaac catacaccag ttaagagtct acaatttgtc tggcggttaa atggtttttc
      961 ttctttattt atagcctata cgatgagtat agtgatctat tatatttttt ctactaaaaa
     1021 taattcatgg aaaataatgg tctttacgtt tcttgcacta atattgcatc tctcaggagt
     1081 aagcaattct atccatgcta acaaagattc attaacactt atagcacctg aagacgctgt
     1141 tgctatagct gaaaattaca atcatactga ttatgcaaac aaagagtcaa tctatcatcc
     1201 tgatatgatt aataatgatc aatatttact aggtaaccag gaaataaatc caacaactca
     1261 ttttatgagt aacaaactta caattgaatt agataattcg aataataaaa atactgtctt
     1321 gacgacacct atttatcgtt ataaagggca agtggcttct attaatggca aactagtaca
     1381 aacaaaatta tctaagtttg gcacaactga attaactatt cctccaggta taaataaagt
     1441 cgttattact tatcagtaca caaaactagc aattgcttca agatatttat ctatcgttac
     1501 tttgatttta tttttacttt atcgatttac tttttctaaa caaaaacaac cgagaagaga
     1561 agtacaacat tctcactaga tttttattta agaaagactt tttagctgta cacataaaca
     1621 ggctaaaaag tcttttttta tatttcttta atttgcttca tacattattt aacatcgata
     1681 tttatactgt gaaacatgcg tttcatccat tatcaaagta tttttatcga taataaactt
     1741 catccaaaaa cagaccttgt gcagggacgg tttctcctgc atgttgccgc actttgcttt
     1801 caaatatttc atcgattgtt tctatcggca tggcacctgt gccgatttct agtaatgtcc
     1861 ccatgataat ccgcaccatt ttatataaga atccgtctcc gacaaaagta aaatgaagca
     1921 tttctccctt tcgttcgata gataattcat caatcgtacg cactgtggat ttttttgatt
     1981 ttttcaatgc agaaaaacca atgaaatcat gtgtacccac taatttttcg caagctgcat
     2041 tcagcttgtc catatccaac tcttgaggga aataaaagct gtggtttcgc tcaaatgctg
     2101 aaggaatcac gttgttccaa acataatagc tgtatttttt gcccaccgca ttatatcttg
     2161 aatggaagcg ctcaggcatt tcttcgattc ttttgatgac aatatcccgc ggaaggtaac
     2221 gattcataaa gtcgatcatt tctgccagtc caatttctgt gtttgtttta aaatttgcta
     2281 cttgtcctcg cgcatgggtg ccagcatctg ttcttcctga tccaataatt tctatctctt
     2341 catttgtcat ctgatgcaat actttttcga tttttccttg aatggtttta tcgtcatctc
     2401 caagtctttg ccatcctgaa tatctttttc cgtcatattc gattgtcaac ttgatgtttc
     2461 tcatatttta gtggctcctc tgtcttccat ttggtcttct ctctaccttt ttaagattgt
     2521 agcacaaaat atagctcctt gcttctctaa gacagaagct atacaagaaa actgtggaaa
     2581 tatatatttc agaaaaacaa aaaacagcca ccggatgaag tatccgctga ctgttcaatt
     2641 tgacggctca agttttactg tttgagatgc aagatactca accaggcaat gggcacataa
     2701 ttaaagaaaa aactgattct ttggcaaaaa cctattttat tcagtaatgg ccaagaattt
     2761 atagaaggaa ttcttgccag tccataaatc gccgcaaaaa gcaaactgat cacagacagt
     2821 ccaaggtata agtggcttac gtccatttgc ccattttttc gatagatcaa gaaaagaaga
     2881 actggaaaca ataaaaaacc actgtaacct aaacctgatc cgatattatg gacccatgtc
     2941 gagacattcc acgaagattc atgggtattg atactgaaca aacctgtaaa gatacaatca
     3001 cctataccat agaacgcgat ggctgctgta actagggtag ctaacacacg agatgtacta
     3061 acaaatgcag catacaatgc aggcaatgac aggatgaaaa agatccctga aaccacagac
     3121 caaataagaa acgcttttct tacaggactg gaaacctctc caaatacact gatcacgctc
     3181 ttcatttgat ctaattcagg ataatacaat cctaatacgt acggagtcaa aaaatcactc
     3241 agcacgccta ataataaaaa gaaaaaacca aatttacgta aaaaatccat gatacgcctc
     3301 caataacaat tacctatgct tttaattgtt attataaacg actttatctt gatttaacag
     3361 aaacctctat ttacacgaac atctatataa ctcattaaaa actcgaatca ttttaaaaat
     3421 actaaatatc taacgttcaa cgttcactta atccatctaa acaatctttc aatttgattt
     3481 tttccccact tcttttacgc gcgcctagct ctcctattgc agtaaacaac agatcacttg
     3541 acgaatttaa ggcagtttcc attgaatcct ggataacacc gataataaat ccaataccga
     3601 cgatttgcat cgcgacatca ttagagatac caaacaaact acatgctaat ggaatcaata
     3661 acagtgaacc gcccgcaaca cctgaagctc cacaagcagc taaagcagac aaaatacata
     3721 aaaggacttt caaaacaaaa ggtgcttcaa tccctaaaga atgtacagca gtcaacgtca
     3781 tcaaggaaat ggtaatcgcg gcaccgccca tattgatcgt agcacctaac gggatcgata
     3841 tggaataaga ctcttttgtc aatttcaatc tttcagccaa catcatgttt atcgggatat
     3901 ttgcggcaga acttcgggta aagaaagcgg gaagtgcgct ttctttcaaa caaaagaaaa
     3961 tcaatggata gggattttct ttaataaaca aaaacgtgag gaatgggtac acaactaatg
     4021 cgacaaacag catcgtaccg atcaaaacaa gtaaaagctg cagatactct gccaatccag
     4081 cgattccagt cgtagcaatt gattgataaa ccaatcctaa aatccctatc ggagcaaatg
     4141 caataatcac ttgtacgact tgtgacaacg ctatagatat ctgatccacc aactcttttg
     4201 tcgattctga acgtgctcga aaagctagtc ccaacaatac agcccagaat aagacgccaa
     4261 ggtaattcgc atcaataaga gattgaacag gattttgtgt gacattcgtc aacatagtag
     4321 ataaaacact ttccaaactt tgcggtgctt tttcagatac tgtttcttgt aaagtaatag
     4381 gaacagtaaa aattgaactt acaataactg ctacaagtgc agaaagcaat gttcccacta
     4441 aatataagat caaaataggc ttcacgaatg tcttattccc aattttatgc ttggcaattg
     4501 acgccatcgt caagaaaaaa accagaatag gtgcgattgc cttcaagcaa ctgacaaaca
     4561 acgttcccaa tattgaaata aatgtccatg tggggaaaaa gataccaaac aatattccga
     4621 ctaaaatacc aacaacaata cgttgaacta acgacatatc caaaaacttc tctaacaaat
     4681 gaccaccctt tcacaatttt aacaatcatt gtatcatttt atgcaaagaa actaaaggta
     4741 taattttcta aaaattctaa caatacaaac aaccatttct tgctaatttt gtactttttc
     4801 tgtctacacg ttgttttttg agatattcta agttaggatg ttattaatgg gcaacttatc
     4861 aggaaacgtt ttcttatgtt tttgtccaat ttttttgtga ggtgtttatt cttgaaaaaa
     4921 ataaagaagg agagttatgg cttcctgaca atctttgtcg cttgtataat gatggtttta
     4981 cagcgtatat tgatttcagc agtcactatt cctacatatg caacaaaccc tggtcgattc
     5041 tacatctata catttttaca aacttccgtt gttattggtg caaatttgat cccactttac
     5101 atgggttacc aatatcaaaa aatcgcccgt ttaaaagttt tacattattt aagtcgtttt
     5161 gcctttatat tcctaatagc cacactagtt tgcaatatct ttttttacgt acaagctggc
     5221 agcttgaata ttcgtgatta ttggcttatc ctaacaccta tcagtcagaa ttactttact
     5281 tatgctgttt cttgtacttt gcttttttgt agtattcctt atacagttgg atttttaaat
     5341 aaattatcga aaacaacaat taaatatcta ctattatttt taacaattgg attagttggc
     5401 tgctctactc tatttaataa agacccgttt gcattacaga atggacagag tattttatgg
     5461 attttttatt tatttttaat gggttatggt ttgaaagaat ggaactggaa agaaaaggtc
     5521 cgctataagc ttcctcaagt atttatcagc ttgattgttt tattcggttt gattattttg
     5581 atgacacaaa tttcactcat catcagagga aatacagata cagcgctgag gttttctaaa
     5641 ccatattctc tcttcgcaat gtattatact ttctcttcgt ttttattttt agaaatgcta
     5701 tctgataaat taggtataaa aggaagagga aattttcttg ctgtattttt gattgtcact
     5761 caagttatat gtaatttccc tttagtttct tatcgcgtat ctacatttat gaaacgtgaa
     5821 tttccaaact cagggaaggc atggctgata aatatcgggc agttttttat ttgatattta
     5881 ttagcagtgt ccctcctagt tatcttttta ttttatatac aaaaaatccg tttcattcat
     5941 cgtttcatcg aatacttcgc ttttgattcg attgaacaat taactcagcg ttggaaaaaa
     6001 agaattcatt ggctttttgt tcataaaagt tattttttag ttgcagcatt tttttactgc
     6061 tttaccttcg tgcagatttt cgttttagaa ccaaaagaag gttggaaaga aactatccag
     6121 gttgccttaa aaatatttac tcagcgacaa gcacccatga tcctgacaat catcattatt
     6181 atgggatttt tcttcttact attgttgatt tccaatcgtt tttggtacgc cttgactgct
     6241 accttgatca ttaacttact gctcactatt tcgacagtaa tcaaaatgga aatgcgggaa
     6301 gagcctgtct ttccttcaga tttaaaaatg ctgaccggat tgtcagaact attatcgatg
     6361 gttagcccag tcttattaat cgtgggcgga ttgatccttc tcctattatt gattactagt
     6421 attattgtac aacgtagact acaaaaacag tattccttga agattcattg gaaacgtcga
     6481 tttatcagta tcgctgtatt attaggatgt ttttcgggtg tctttttcat taatcacaaa
     6541 aattcgcctt ctttcttgct gtttaattta tttaaagtaa acaagacttt ctttaaccag
     6601 caaggcgccg taaaagataa cggaccagtc attcaattcc taaacaatat cgacattaaa
     6661 atcatggaag aacctgctgg ctattcgaaa gaaaaaatta atggaataat gaaaaagtac
     6721 aacacaaagg cagatgaaat caaccaaaca agaagtgact ggttaaacaa tcagacgatc
     6781 gttctcaatt taagtgaaag cttcagtgat cctgcacgtg tccctaatct gacagtacct
     6841 acagacccta ttccttacat caaaagcatt ataacagaag gaacaggagg attgatgtta
     6901 tccgtcggtt atggtggcgg aacagctaac atggaatggg aaggactgac aggcttgagt
     6961 atcagtaacc tgtctgcttc gttagttaca ccttataccc agctagtgga gcgtcagaaa
     7021 ataagtccaa atattaccga tctattcgat gaaaaaatag ccatccatcc ttttacagct
     7081 gctctttatc gcagaaaaca agtgtttgat aaatttggtt ttgacaggtt ctattattta
     7141 gacagtccgg ataaattaac ttatacggat aaaatagaac aaagcccacg catatcggat
     7201 gattcagcat acaaagaaac cttgaaagct ttacaaagca atacggaaac gtcacaattc
     7261 atccaattat ccactatgca aaaccatatg ccttatgatg attactatag tgaattgaac
     7321 tacactgctg aaggaactgc tgtcattcct aatagaagaa aagaattaca aacttatatg
     7381 caaggccttc actataccga tacagcagta aaaaaattca tccaagaaat cgacaaaatc
     7441 gataaaccga ttacctttgt tttttatggt gaccatttac ccgcaatcta tagtgggaat
     7501 gattcgaaaa aatatggatt agaacagcac cagacagatt atttcattta taacaacaag
     7561 tatagtcgtg aacaagcagc tacgttaaat aaagaagttg ttgctcctaa taactttggt
     7621 gctatggctc ttgagcatgc gaatatcaaa gtaactcctt actatgcgtt gttgacagaa
     7681 gtatctaatc atctacctgc attaacaatt gatcctacag aaagcttgtc taatcgtttt
     7741 aatggaaaac aggtctttgt tacagaggat agtcaaatta tccaagaaaa aaatttaact
     7801 gagaaacaaa aagaaattct agaagattat cgtctgatac aatatgatct tgttgcaggt
     7861 aaccagtaca gtgcaacatg ggcgacacaa aaaataaaag actaacaaat aaactgagca
     7921 aggggttgtg ataaaagaca tgtcacaacc ccttgtttat ctcattaatt tgcttgtcca
     7981 taaacgattt gattaaagcc atttttcaat tcattattcg ctgtctcgtt gaagtaaaga
     8041 taattatcgg gattcccctg attttgttgt gtcaccaata cttttggctg attttgctga
     8101 aacccgaata gatagacgtg ctttttcaga tatggttctg tctctgcatc ggaatagacg
     8161 gctgtgattt gatagtctgc ttgacctgtc ccactttctg accattctgc agaaacaggc
     8221 actccaccaa tagccatctt ccactccccg ttcaaaatag cttgagggac tttaagacca
     8281 tataagtcca cactcatttg attcgtataa cttttgtatt gttgtcccat tgtttggccc
     8341 catttggtca taaatgcctc taattgtgcg gatttgtctg cattccacag tgtttgttca
     8401 acttgttcgc tgctttctga agaggaagat gaagcctcta cagaagaact tgaagtagat
     8461 gtttgagttg tttcagtcgt tttggaagac tctgacgaag tagaggtatc tgctgtttct
     8521 gtacttgctg cagttgactt gctctctgtg ctagttgttg tagtagttgc aactgttgct
     8581 ttcgctgttt cttttgttga ttcttttttc gattcattcc cgttggtaca accagctaac
     8641 aaaaaagcta gaccaattgt tactaaaatc ctttttttca attaaaaaac tcctttcgat
     8701 tactcccact tctttattct atcaaataag caatgtgatt ttattgattt ttcgtatttt
     8761 ctcatcaatc tttcaaatct tctaaaaact gagaagcacc cgctgatgtt gtctgtaata
     8821 catcagcggg tgcttctttt attttaatct tcgtgatttt ccaatttacc tcacgcttta
     8881 tatttgtatt tgaagaaaca tagactttta gtcatcatcg tcttcctcat ccaaatcttg
     8941 tcggacagtt tttccttgga gtctttgtac ttggtagatg atttctttat tgaaacttaa
     9001 aagttcaaca agcaattgtt gcaagccata tttttcttct ttttcagcta atttgattgc
     9061 ccgtgtgata aagacatttt ggaaatcaaa atcttttgca aagttttcca acattgttga
     9121 ggctggtaaa tatttgtttt gaccatgttc gtaaattttt ccataacgca tgacttcttc
     9181 taaagtagac ggaacgattt cattttcaat cactaactca tgagagattt tattgaacca
     9241 tctcgcttct tgatcagcat attctgaaaa aagggtactt aattcttgcg aagatgtacc
     9301 caaaacatac aattgtgctt ggatcaattt catttcttct aatttaaaat ttgctaatac
     9361 atgccccacc atggcgccag cagttggttt atgatggtcg atatctgctt gttttgtttc
     9421 ttgaacaaat ttttcttcca ctatattcat tgatttacgc ctcttttact tttgctgatt
     9481 ggacttcata acctaatttt tccacaactg cagctaattg ttcagggtgg acttcatctg
     9541 gatcaaagtt tgctttgatt ttgctcgcat taaacaaaac tttcgtattg cttaccccag
     9601 gctgttgaga aaccgcatgt tcgatttttt gcatacagct tggacaagct agtgtatcta
     9661 attgcataat aatttttttc atcgtagttc ttccttcctt tttcttgata gtcttattct
     9721 agattacagc ttccataaaa aaattgattc ttatcaagtt ttacgatttt tcttctttgc
     9781 tttcgtgtcc aaaatacaat aaacgcatcg cgttaaagat aacgatcaag atactagctt
     9841 cgtgaacgaa catcccacta gccatgtaaa taaacccggc aaataagccg ttgagtaaaa
     9901 ataagacgac accaatggca atcactacat tttcacgggt atttaaaaca gttttcttag
     9961 ccaagcggta ggcatgaacc aacgattcaa aattagattg catcaagaca acgtcactcg
    10021 tttcgatagc aacatccgtt cctgatccca tagcaatccc gatatcagcc gcagccaaag
    10081 agggagaatc attgattcca tctccaataa aagcaacgcg caatccttgt tcttggtatt
    10141 ttttcacaaa ttgaaccttt tcgtctggta ataattcagc atggacttca tcaatgccta
    10201 acatatcagc tacataatca gcagtcatct gattatcacc tgtcagcatc actaactgtt
    10261 ttgctcctgc ttgtttcaac ttagctaatt gttcagcgac ttctggtcga atcacatctg
    10321 acacacctat gatttgagtg atttttttgt cgattgctac aataatgacc gagctgcctg
    10381 tttgttgtaa ttgatgtaaa tcttgtactt gctttggtgt gagagaaagt ttgtgagcgg
    10441 caatcaattt ggtgttgccc gcacaaactg tatgaccatc aatctcagct gtgattcctt
    10501 gtcccttgat tgtctgatta tctttgactg ctaattgttg aaaatcaatg tctttctcct
    10561 gagcataatg gatcacggcg cgccccagag gatgatcgga taacgtttct aatcgagcca
    10621 ctaaagctaa gctgtcccct tcatcctctg tatagttctt gatagtggat acagcagtag
    10681 ttcctttggt caatgtacca gttttatcaa agacaaaagt atcgattttg gaaaaagtat
    10741 ccattgcttc accgcctttg actaagatgc cccgtttcgc tccattaccg attcctgcta
    10801 cattcgaaac aggcgcacca atgactaaag cgccaggaca accaagaacc aaaacagtga
    10861 ttgctaaacg aaaatcacga ctaatcagac caacaaccaa cgcaataagt aaaacggttg
    10921 gagtataata ctgagaaaat cgatcaatga atttttccgc atgggatttg gtatcttgtg
    10981 cttcttctac taattcgata attttggcaa aggtcgtatc atctccgacc ttagttgctt
    11041 caatcgtcac gtagccatct gctaaaatcg ttccagagaa gacttcatct ccactgattt
    11101 tgacgacttg tcgtgattca ccagtcactg cggcttcatc cgcataccct ttcccgtcaa
    11161 tgactcgtcc gtccacagga atagctccac cagtttttac taagacgata tcgccttctt
    11221 ccacgtcatc gatttccact tcttcagtcg ttccatcttc ttggacaaca ctagcagtag
    11281 atggggccat ctcggtcaaa gccttgattg actcccgcgt tttggtcaat gttttttgtt
    11341 ccagatagct accaaacaaa aacaagaatg taacgatagc tgactccttg tattctccga
    11401 tcataaaggc accaagcaca gcaatagtaa cgagtaaatc aattgagata actttgactt
    11461 tcactgcctg atacgcatgg atcataatag gtatgaccgc aataatagaa gctatggcta
    11521 gagcaatgtt atagaacaag acaaaaccag taaattttcc gataaaacca aaaataatca
    11581 acacagctga taaggcggtg atatgatttc gatgctcttg gatccacttt tgtaatttca
    11641 tctttctccc tcttttcctt tcattcttac gagactaagt atagatactt atccatcata
    11701 aaaaattgac cactatcaag tttagtcaaa aaggcggttt tcttttggta gaatacacta
    11761 caaaaaatag ttagcaagaa gactcactaa aagtgatctt gccaactatt gtcgtttcta
    11821 tcgaaatttt cattgaatga ttttcctgta cagcatacat cggagatcta tttctttatc
    11881 taagattttt atcttgctaa ttttttcctt tagtctttca aatccacgtc gttcatagaa
    11941 ttgatacgta cagccactat ctgtaaaaag gaatatttct tttcctttct ctcttttttc
    12001 aaattctgtt agcaacttgg taccgatgcc ttttgttttc aaatcaggat ttgcagctaa
    12061 aaaaatgatc tctccatctg gcgaatgctt ctttaaatat gctgcaagca tttgttcatt
    12121 tgctgtctcg taagctcctt tactttcttt agcaaatata ttttgtaaaa aatcgaatat
    12181 cttaacatat atttgctgtc caaaagaacg atgtttcttt ttttctcctt ttatctccgc
    12241 cagcaaaaca cctgccaact cgtcctctgc atacaaagca agaatttgag ttgctcgatt
    12301 caactccata tacaaaaaat atcttccgta aagattcagc agaagttcat tttccatata
    12361 tctctcaaag ttcattcctt caacagcaaa atagatagct tttttgtaat ctttcttttc
    12421 taagggttta aaagttagtt tcatcatttc gcctcctcaa cttcaatgat tgtgttatac
    12481 ttacattata atagacacag tgtctactat ctataatcaa tcatctataa gtttgtcgat
    12541 taagcgacag ctttgctttg atatgtcgga taaggagata ggtaaatgaa aaagacagat
    12601 agacgcgtca aaaaaacaga aaaagctttg gctgaaacac tatccacatt attagtaaat
    12661 aaaaagattc aagcaatcac tatacgcgaa ctaacagaaa cggcagatgt acatcgttcg
    12721 actttttata cacattataa ggatatctat gatctatatg atcaattaga aagcaacttt
    12781 tttcgggatc taaatgaaat tctctcttat gatccagccc atagctatga ggaactatac
    12841 actcgcttga ttgactatgt ttacgcaaat acttttttat atcgcttatt tatcctcaac
    12901 catggtgata tttcgtttca gaagaaagtt aaacaagtta ttgaagaaaa ctatctgaac
    12961 atatggctat atgaagacaa gaaaacgatt atcacagaag aaatgagatt ctttactacg
    13021 taccacgtcc aaggttgtct atccgttctc aatcggtggg tagaaagcaa ttacaagtac
    13081 tccaaaaaag aattattaga actcttacag aaactgaatg atcacataga gaaaatcatg
    13141 ccttgaataa aaacatacta agcttagtta agaagaaccg taaccttctc gtataacaac
    13201 tgaatcttat ttagccaaat tgtttactga agattttttg agtaaaattt ctataattcc
    13261 tacttattga aaagtagttt gtagtgctgc ttctccctct ctaagtgata tatcttgttg
    13321 taccaatcaa attcacctca tcattaataa attactttta ggctaattta ttacaactta
    13381 taggttgaag ctgtaaccaa tttatagtca tattatattt ttatcatcca gtttattgaa
    13441 ttcttctcgt ttacctcaat tcattttcaa ggggaatcag ctcatcatct aacattttac
    13501 gccatgctcg cttcatttct tgtgggtatt taaatggttt cgcttctgga tgtttggctt
    13561 tatatccttc gtaatcctta ctgttgataa ttgctttcat attaggaatt ggatcttcac
    13621 ctggcatata ttgcagatta gaagcatgta attttttctc agaaacaaac cactcattgg
    13681 aaaagacatg aatcaaatca tcacttactt gtgtaaaata gcgacgttta atgacaaaga
    13741 tatcttcatt gctatctagt gtaccttcat ttctttgtgc ttaaattcaa agataatttt
    13801 ctcaataaag atttttgatt ccagtaagat tcctttactc cttttacaac atagcgttgt
    13861 cctttgtttt gaaacatctt cttcccctct ttgccattaa aaatagccca attctctata
    13921 aagaagatta agcttcatta ttataatata aagtttaaat ttggtttatt acttactttt
    13981 tcttattctc tagtaagcta atattgaaaa aaatgtaatc agatcattat ttttatatgt
    14041 tgtaacttcc tgataagaaa actaattaaa agaaagaggt aactacataa actaattaaa
    14101 aaagctaact tcatagttgg aggataaatg gaagaaactt caatgattgt ttctccttgt
    14161 gttccttcaa tcgacactag tcctattatc aatctctctt ttttgtccat ttacttttgc
    14221 gactgttccc caataatcaa atacaggaag cgttattttt tgtcctttag attcatgtac
    14281 tgtatagact attttcgaat ccatcacttg ataaggcata gtatgccatt ccttgccaat
    14341 tcttacttca tgattctgca aatgttcttt gtattgtaat gcttccgtgg gtgcatagtc
    14401 ataaagtcct ccgggaagga gtgtcgtgta acgaataaga ttttcttgat tcattaccaa
    14461 gtgatctctc tgagtgacaa ctttatgttc atagtttgct tttgttgcag cagatacagc
    14521 caacagtaat aaaacaattg ccgttatatt aactactttt ctttgcttag ctgatgtgct
    14581 cttcatgaaa acgaacgata aaatcaacga gccaaacagt acttgaaagc ctaagattcg
    14641 ccaaggaaat tgaatcattt gaatgggtgt attttgtaat aaagaccatg gaaaaaattt
    14701 ggtggataag agccaagaga ctaccgcact aaggtaaatc actttttctt tgcgattaaa
    14761 tcctttaaaa aagaagagtg gacagatcaa agaaataata ccaatgattc ctaccgtaaa
    14821 ggttgtcagg tcattattca tcgcaccaat caaactatcc gagacattta atgcttggtg
    14881 ttgtaaggtc gttttatctg gctcattaat cgattgaacc atgatttgtc ggaacatcgg
    14941 ataccaaaag tatgctgtta aaagaatcgt cattcctact gcctttgaca actccacaat
    15001 caaccggatg ctttcttttt ttcttgtttc acttactaca aagccgccta agactagtaa
    15061 gccaacggct attacactca taaaagtgga aagaacatgt gtataaatga gtaagctcat
    15121 actgactgct aatggaattg cacatggctt tttctcataa atcagtgcgt aagcataata
    15181 cataactatc ggtataaccg taagtgcaat cgcttcagca atagcagaac gatgataaat
    15241 atcaatcgta cggtacccat aaaatgtata taaacagcta aatataaaag ccgcatcgat
    15301 tctttttaaa aattttagtc cataatgaaa catcagcatg atagtacaaa cattcaatag
    15361 ccaaacatat aagatataac taacgattaa attattactt atccaataaa aaatgactgc
    15421 cggaaaaaag gtcaagtagg gataaaaata attcactccg ctcccataat gattaaacgt
    15481 cgtaaaattg attggaccag aaaaaattga agataaccct ttgactctag caaaatggaa
    15541 ggcaatatcc cccgatgtag aaatagtatg aacatttcta aaataattga ttgaataaag
    15601 tccagcaata attgctagga gaatggacaa tattattttt ttctttactt gcattactta
    15661 attatatact cctttcatgg attgctttat ctagtataac ttaaatttta aataaatcgt
    15721 tatttatttt tactctcact taaaaaagca ctaaaattat aagagaggga ggtaatggtc
    15781 tgaacaccaa gaattgttcc aacatcgaat tgcttaacta atgagttcaa agcttaatta
    15841 tgtggctctt atatttaccg agcgtggatt gcgatctctt tagcgaaaca aacatctttc
    15901 ataaaaaaaa cagccatatc cgctttcttg gatatgacta tttactacta ctttactatc
    15961 tttcattctt cttgtctcac ttatgataag gcgcccccgc attgatccgg aaggcacggt
    16021 agatttgctc gacaaggatc agacgcatca attgatgtgg atacgtcatt tttccaaaag
    16081 agatctgagc attgcttctt ttcatgacgg cttcgctgag tcctaaagag ccgccgataa
    16141 caaaaacaag gtggcttttt ccttggatac ctaattggtc gatttgttta gcaaaagatt
    16201 cacttgtagg atttttccct tcaattgcta aggcatagac gtattcttgt tctttgattt
    16261 tcgctaagat ccgttcccct tctttttctt ttacttgtct catttgcgct tcgcttaaat
    16321 tctccggggc tttttcgtca ggaacttcga tcaattcgat ttttgcatag gcattcagcc
    16381 gtttgacata ctcattgata ccttggatca ggtatttttc ttttaatttt cctactgaga
    16441 taattttgat attcatgttt tctctccttt ggtcttcatg ctcactttgt ttattataat
    16501 caattttttc gtctatatgt ttattttggc tatttttgtt aaaaagattt ccccaacagt
    16561 tatgcacaac atccacaggt ttatccacag aaaacagtgt tattttacct tatttcatca
    16621 cattcattag gcaaacatac gttttataag aatttatcca ctttatacac aggctgtgga
    16681 tatgttttat cccttatttt tccaatccta tccgcgtagt tttacacagt atctgtggaa
    16741 aaagaaaatc aaatattttc atctgaaaac aatgattttt cctaaagttt ttcataaaaa
    16801 gttcacagtc tcaaagtaga ctaaagtcaa caaataagac tatactaaca gtagataaat
    16861 gaatgtaatt ggaggaggaa cacatggata gaaaaaatgt gacaccaaag atgaaaaaaa
    16921 gtaaaaataa tagcctctgg cgtaagttag gtctggggct tgttggaggg atcgttggag
    16981 gactactgac agctggcatt ttttatgcag cgatgggcac tggcaacaat agttctactg
    17041 ctacaagcgg caatcagaat tctgccggtg aaacagttgt ggaaaatgtc aaagtcaatg
    17101 tagattctga tatcaccaaa gcggtagata aagttcaaga cgctgtcgtt tctgttatca
    17161 acttacaaag tcaaaaccaa tcaagcggtt ttggcggact attcgggcag caagaagaaa
    17221 gtagcagctc agatgatagt aatttggaag cttatagtga aggaagcggc gtgatctaca
    17281 aaaaagacgg gaatactgct tatgtagtca cgaataatca cgtagtagat ggccagcaag
    17341 gtcttgaagt cttgatgaaa gacggtacga aggtaaaagc tgaattagtt ggaacagatg
    17401 cttattcaga tttagcggtg ttgaagatca attcagacaa agtcgaaaca gttgcctctt
    17461 ttggtgactc aagtgctctg aaggttggtg agcctgcgat tgcgattggt tccccactag
    17521 gatctgaata tgcaaattct gtcacttcag gaatcatttc ttccttgaac cgacaagtga
    17581 caagtacgaa tgaatcgaac gaaacagtca atatcaatgc gatccaaaca gatgcagcaa
    17641 tcaaccctgg aaattctggt ggtcctttag ttaatatcga aggacaagtt atcggtatca
    17701 actcaagtaa gatcgcaagt acttctgaat ctacttctaa tgtaagcgta gaaggaatgg
    17761 gatttgctat tccaagtaac gatgtagtga atatcatcaa tcaactggaa aaagacggaa
    17821 aagtgacacg tccagctcta ggtatcacaa tggtcgactt atctgccgtt tctactcagc
    17881 aacaagaaca aatcttgaag attccttcat ctgtaactaa cggtgtcatc gtgacaaatg
    17941 tccaaacagc tacaccggct gaaaaagctg gattgaaaca atacgacgtc attactaaga
    18001 tcgatgacaa agaagtttct tctggtgtag aacttcaatc tgctttgtat aagaaaaaag
    18061 tgggagattc tgtcaaaatc acttactatg aaggctcgaa agaaaaaaca actactgttg
    18121 aattgacgat cgatcaatcg gcattaaaac aaaatcaaaa tagtgaaaat taataaaaaa
    18181 gaggttgtga caaagacttg tcacaacctc tttattttgg aaatgcttct ccgactacgg
    18241 catcctcagg caatactaag attccttttt ttgctggagc atttggcaaa tgcaattcac
    18301 gagcggaaca gatcattcca tagctatcta cccctcgcaa agttcctggc cagatcatca
    18361 ttccatctgg catcatagct cctggttttg caacgacaac tttttgtcca gcttcgatat
    18421 tgggtgcacc gcaaacgatt tgcaatactg taccattgtc tacttctgtc tcagtaatcg
    18481 ataaatggtc agaatcagga tgtgggacgc atgtttttac gtacccgaca acgatcttcg
    18541 gttcatgatc agcttcaagc tgttcttcga atcctgcttc tttgatcaat cgattcaatt
    18601 tcgctaattc ctcatctgtt aagcttactt ggccgttccc ttcgattgtt agatgatcgg
    18661 agatatggaa aaagttccaa gcaacaactt gaccttgttc atctttgatc cgagcaacat
    18721 tccatttgcg ttcaacagtg ttttctgttc ctttatcgtc ggctataata accatcaagg
    18781 tgtctccgac gtgtgctttg ttataagcaa aaatcatgtt ctttcctcca ttatcctttg
    18841 ctgagattta tcttaacaaa aaattgcatt cactgcatga aatttttttc atgctttatt
    18901 tccgaagaag cctcttctgg aacaccattt attcgtaaca agggactgtg acaagacttt
    18961 tgtcacagtc cctttactgt tttagttaat tgattaaaaa ggtcgtacaa gttccacttg
    19021 cttaattact tggctgttct tcttcgtcag aaacgatcaa gacatcacaa ggtgaatgac
    19081 gtatcacgta gctggctaca cttcctgtca taaagcgttc gactgcattt agacctgact
    19141 gcccaaccat gattagatcg atctcatatt tttttggcaa tttgactgcc atggattcac
    19201 gaacagaacc atatgcaata attccttcta tatttgtgta atcaacagat ttcccatatt
    19261 ctttacattc ttcgatcatt tccttggctg tttcagtttg ctgatcgact agattttgat
    19321 caataggtgc atagcccata aagttataaa attgctgatc gattacgttt gctacatata
    19381 ctttcccatt attacggcga gccacttcta ccgctttttt gaaagcagca agtgcttgtt
    19441 cactaccgtc gatacctact aaaatattct tatattcttg attcatcttg acccctccta
    19501 acagatgtca gccttttgtt acactttcta ggaacgattc gatttcctgt tttgttttac
    19561 gatctttatt cactagacga cctaattctt ttccgtcttg gatcacgaca aaacttggga
    19621 taccaaaaat gttccattca gcggctacat ctatgtactt gtcacggtct acttcaataa
    19681 attgccaatc agaaaagtca ttttcaattt caggcatttg cggtttgata aaacggcagt
    19741 ctccgcacca gccggcagta aagaaaaata cactctttcc agtttctacg tagcctgcta
    19801 attcttctaa actatttggg ataatcattc tttgtcctct actttctgtt tatgtatttt
    19861 tcaattaaat tatacacctt tctttcgaac taacaaacat tgatcctctt tttataaaaa
    19921 aaatctggca aaaagcaatc ttttgccaga ttttatagtt tcactatcgc attattttcc
    19981 gtatacgatc gtatttactg ttgatttatc caaacctttc agtgtttgaa tcaacatttc
    20041 tcgtgcagct tcaaaatcgc gaatactgaa catcgtttga tgcgtatgga tataacgtcc
    20101 cactacgcca atcactgtac ttgggattcc ttcgttttgt gtatgagcag cacctgcatc
    20161 tgtaccgcct tttgaaacaa agtattgata agggatattg tttgtttcag cgatatccag
    20221 caagtactcg cgcaaacgag gaagcatgat caaacctgga tcttggatac gcatcaatgt
    20281 accttctcct aaatgtccga atgtgccatt tttcgtcact gtatcatctg ctgcagagca
    20341 gtctactgca aagaacagat ctggcttgaa tttattgaca gaagctttag aaccacgcaa
    20401 gcctacttct tcctggacat tcgcgcctgc aatcaatgta tgacctaatg tttcattttg
    20461 caacgcttct aaagcttcta ataccattgt acagccatag cggttgtccc atgatttact
    20521 gataatattc tttccgttgg ctgttttgat cgtttcagcg taaggaacga ttgtgtctcc
    20581 tggaaggaca ccgtaactca ttgcctcttc tcttgattca aacccagcat cgaacaaaat
    20641 atcggttact tctactgatt tttgtccgct tgttcctcgc aataaatgtg ggggaacgga
    20701 agaagaaata catggataat tcttaccagt agaggtttta agtgtaaaac gctgagcaga
    20761 aacaacgtaa ggattccagc cgcctagagg tacgacttta aaaagcccgt tttcttggat
    20821 ttgtgtcagc atgaatccga cttcatccat atgtgctgcg accattacac ggggtgcatc
    20881 cgcttcttca tgatggcgca gaccaaaaat gccgcccaaa ccatctaact gtaactcatc
    20941 taccagcggt tccatatgac tttccatata agcgcgaaca tcttgttcaa aaccgcttgt
    21001 tccttgtaat tctgttaact ctttgattcg ttgaaatgtt ttttcttcca tatgctattc
    21061 gtctaaattt cagactaaac tcctctcaga cttatattga tctgcggata aaatatgatt
    21121 ttttctgcag accgatcgta tttattatag cttatttttt ctggtagcgg ttatttattt
    21181 caaagaaaat atgagagtgt ggtaaaataa gcacaaggag taacaagtga gaagaaggag
    21241 gtatgtccac atgtatgaag aaagcgaact aagctatttt aaaggcgggt tagcagttgg
    21301 aatcggtcta ggattattga gcggggtagc ttcaacgatt tggtatcatc gcaaaaaagt
    21361 attgtcagca gaccaagtac ttgaaaatat caagtcatct tttctaaaag aaggtgcaat
    21421 cgaaggttct tggatctctt ttgaaaagga acctattagg aaattcgccg ttcggtcgag
    21481 tgcttatcgg ggaggtcttt ctcgaataga agatggcgaa actgtttatt atgaattttt
    21541 agcagatgct catacaggaa ccgtattaga aatttcaaga aaaaaaggtg caacagatta
    21601 atacgtcaaa aaagtgagag aacgaatacg cacccactat tcaaaagaga ggtatcatgt
    21661 ccttcttttg tagtgggtgg ttttttagat agagctttcc aaacatatat tccagtattt
    21721 tctttttaaa taaatgatat ttagcgggaa aaactagcat ttcctttgat tatgaggtat
    21781 ttttaattta gcttttgttt tctatagtta aggagagtga aaaatggaac aacaagaaaa
    21841 aattgctgtt ctgattcctt gttacaatga ggaagcaaca atttcaacag tcattgcaga
    21901 ctttaaacga gaattaccag aagcagatat ttatgtctat gataataatt cgaccgatcg
    21961 aacatacgaa ttggccgttc aaggtggagc aatcgtaaaa aaagaacctc gtcaaggaaa
    22021 aggaaatgtg atccgccaaa tgttctttga tatcgatgcc gactattatt taatggtaga
    22081 tggcgatgat acttatcctg cagaagctgt acatggtttg ctcgagaaat tacgttctgg
    22141 tgaagcagat atggtcattg gtgatcgcct atcaaatgga acctactttg atgagaataa
    22201 gagacctttc cacgattttg gaaataacct tgttagaaat acaattacta gaatgtataa
    22261 aacaaaaatt cttgatgtca tgaccggcta tcgaggattc aatcgtatct ttgtcaaaag
    22321 ttttcctatt atgagttctg gttttcaaat cgaaacggaa ttgacgatcc atgctttaga
    22381 taaaaaattc aagttcgttg aaatcccaat cgattatcgt gatcgtcctg aaggcagcga
    22441 atcaaaattg aatacattct ctgacggatt caaagtgatc atgatgatta ttaaaatgtt
    22501 taaagattat aagcctttgc ttttctttgg gatttggacc atcttcttct ttttatttgg
    22561 attggtcacg gggattcctg tgattcgcga atatatgctg acaagcttta ttacacgcat
    22621 cccttctgcg attttaagta ctggattgat gactttagca cttctttcat tagtaacagg
    22681 gttgattttg gatacagtcg tcacgaacgc taagaaagaa tatgaattga atctatatca
    22741 tgtttatcat gaataccaca gtagaaagta aaaaaaggat caagactgca aaaataattt
    22801 gcaattcttg atcctctttt taattaatta tttcgccaaa cgatagatgg catctgcata
    22861 gattgctgtt gctctgaaca agtcatcaag actcataaat tcgtttgctt gatgcattgt
    22921 atcagtatat cctgggaaca tcgctccata agcaacgcca cgtttcaaca aacgtccgta
    22981 agttccgcca ccgatgattt gttcgtagcc tttttctcca gtgtggtctt catagacttg
    23041 caataatgta gccaccaatg gatcatccat tggtacgtaa tgtggttcca ttacgcgagc
    23101 acctcgagta actgttgcgc cttcttgccc cactgtttgt tcgatgtcac tttgcaatcc
    23161 gtctactgtt acaccttttg gaaaacggaa attcatattg atgaagttcg cttcctctgt
    23221 accattttct tcaaaagcaa atagtccagc attcattgtc aatttgccca ttttttcatc
    23281 ttcataagcg actcctagtt tttctccata aaaatcttca tggacataag cagctgctac
    23341 atggataaat tgtttagcag atcctaggaa ttcatagtta tctaagaaca atgccaagaa
    23401 agaacctgca ttgataccag attgtgggct tgcaccatgt gcgcctttac caactaattc
    23461 gatttttaca tacgtattgt ccgcttcaat cgttccgctt actggatttg cttcgatgaa
    23521 ttggtaaaat gcttcttcca tcgcgatagc tgcatctgca cttggtactt gaagtgctgc
    23581 agttgctgtt cctggcacca tattttcgcg aagtccggaa acaaaagatt tcaagacata
    23641 atctcctgca ttgtcgcctt tgaagcgtaa agctaaagaa acatttcctt tttcaccatt
    23701 gatgattggg aattcagcat ctggtgagaa accaaaatct ggttcttctt catgttcgaa
    23761 ataataagcc atatcgcccc agccgctttc ttcatcgctg ccgactacga agcgaacttt
    23821 ttttgatagt ttcaagtcaa gttctttaat gatcttcatc gcatagtaag ctgccatact
    23881 tggacctttg tcatcgcttg atccgcgtgc aaagatcttt ccatccttga ttactggttc
    23941 ataaggatct gtgtcccagc catctcctgc aggaacgaca tccatgtgtc caaatatccc
    24001 tagcgtttcg tctccttcgc caagatcaat atgccctgca tagttatcga cattttttac
    24061 tttaaatcca tcacgttcgc cataagctaa catatgtttc aatgcgtcgc gaggtccagg
    24121 tccaaatggt gcatctggtg ttacttttga gtcatcgcgc tcgctgttga cacgtaaaag
    24181 atttttcaag tcttcaagca agtcttcttt acgtgcttct acttcttttt gccaatcaat
    24241 tgtcataaag ttaatcctcc acttatttaa gtcttcgtgt tcatgatacc acaaattaca
    24301 gaaaaaaata gttcaaaaaa tctgacaatt ccttttctta tctgtttttc tctaaaaaaa
    24361 atatacgcta taatgagtaa aaggaggttt ttagatgctg ataaacgaac aactagtgaa
    24421 aagagtcatc acacctcgtc caaaaatttc acataaagga acatttggac gtatcgtttt
    24481 gattggcggc aatgaacaat atggcggagc aatcattatg agtgctttag ctgccgtaca
    24541 cagcggagcg ggtctcacga ctgtagtaac tgatgaaaaa aatcggagtg ctcttcacag
    24601 tcatctgcca gaagcaatga ttgtagtttg gacggatact gaccagtttc tttccgtaat
    24661 aaagcaagca gatgtactct tgattggtcc cggattgggt acttcttcta agagtttgga
    24721 acgtttcaaa caagtactag aaaaccaatc aaaagaacaa tggctggtga ttgatggctc
    24781 tgctttaagt ttacttgctg ctaatcatct tttgctaccc tatcccgaac aaactgtact
    24841 tacacctcat caaatggagt ggcaacggtt gagttctcta ccgatacaag aacaacggga
    24901 taaagaaaac aaaaagaagc aacaggaaat cggtgctgtg ctcgttttga aaagtcatga
    24961 gacaaaaatt tatggtcacc agcacttcta caccaatcct ttaggttctg ctgctatggc
    25021 aacgggcggc atgggtgaca ctttagctgg gatgatcaca ggatttttag cccagtttcc
    25081 tcaaaatgac caaacgattg cagcagctat ttatcttcat agtttcattg gagaccagct
    25141 ggcaaaagaa caatatattg ttttgccgac acaaatcagc gaagcacttc cgaaatggat
    25201 gaattatttt tgtaaaaaat caatggaaaa cgaggcatga gtcatggaga aatttacagg
    25261 ttatgtgcga gaaccttttt attatgaaac agatcaaatg gggattattc atcacagcaa
    25321 ttatatccgc tggtttgaag aggctcgggt agccttattg gtacatctag ggtttcccta
    25381 taaaaaaatc gaagaatctg ggatcatcat ccccgttctg gaagtaaatt gcagctataa
    25441 agaaatgatc cattacggag aaaaagtgag catccatcca actatccaaa agtatacggg
    25501 gacacggttg gattttagct acgaaattgt gggaactgaa gacaaaaaat taaaaactac
    25561 tggcagtagt aaacactgtt ttttactttc tgattcgcaa cgattagtga agctgagcaa
    25621 agtcaatccg gagttagatc agttattcca agcgtacgct tctttggatg atgtaaattc
    25681 ttaataaaga ccgtctaagc atttttcttg tattgtgtcg agtagttcgt ataatgaaaa
    25741 gaaaaaagca ggtgatcccc atgtatgtcg ttaaagtaat gcacggttac atcgataaaa
    25801 ctggctgtcg cactcgggaa aaaaacttag acaatctgct cattttcaaa gacaaaaaag
    25861 aatccgaagc ttttgctaaa agaatcggcg gtcgtgtcaa accgatccaa gaggtacgac
    25921 cagattgatt caatattttt cttttcgagg ctctaatagg catgcctatc agggcttttt
    25981 ttgcagactg tgtttttatg cccacacgat ttgtaagcga tttatgttat aatagattat
    26041 atctgcatag aaaggaaggt taaatggtta tcaataattt gcaacagcaa gcttatgaat
    26101 ctatacgtaa acaaatcatt tattccgagt tagcacctgg aagtaaaatc tccgataaag
    26161 tgttagaaga acaactagct atcaggcgta caccgattcg agaagcactg atccaactgc
    26221 gcaatcaaga gcttgtaaaa accattccac agtccggcac ttatgtttca aaaatcgata
    26281 tccgatctgc ttcattagcc cgttatacgc gggaaaagtt ggaaaaaccg atcctgcaag
    26341 aatgcagtgc gaaaatgacc aagcaagatc aagctaaact agaaaaaata ctagaacaaa
    26401 ccgatcaggc agtaatcgct caaaataaaa aagctttttt tgatttggat actgattttc
    26461 accgaacttg ttatgaaatt gctggaaaac gagaaatttg ggattggcta gaaagttata
    26521 gtacgcactt gaaccgtttc agatggttac gtttgacgat ttcagaatta gactggggtc
    26581 gagtattaga tgaacatcag acaatgcttc aatcaatgat tgatcataac tttgatgaag
    26641 tcggatttct ttgcacgatg catttacata tgattatcga ggagcaagaa tacgtgattc
    26701 ataattaccc cgattatttt gaagatattc aggacagacc catcaaataa gaggttttgg
    26761 aatcagcgtt ttgacctgag tattagagaa agaaacggca aaatagttcc tcatattttg
    26821 ccgttttttt tgagctaata ccgagggtca gctgattgca caccgtttat tgagaggttg
    26881 tgaaaaagct gccctgcatc aagtaataag aacagccaag taaaaatagc ttcttatgtt
    26941 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt ttccaagagg
    27001 tcgtgagcct gccgttcatc tccgagtcac aaggaacaat ttcaaaaaac agcttctcat
    27061 gtttgttgaa tattttgact tgtgaccgag gagttgacag gagaacaccg tttattcgga
    27121 aaaaaaggac tgtgacacga cttttgtcac agtctcttta tttgtaatct aaatcatact
    27181 ccgtacatct tttttctatt ttttttattt cttctaagat cccttggcta cgaatttcat
    27241 tatatggcca aagtaagact gcctctaata cataaggcga aaaatcgctt tgattcatct
    27301 cttttgaaaa actcatcgct gctatctcgc tcaatgcacg aatgtttgat tgatgtgtat
    27361 aaaaaggcaa ccgccacagc acaatttttt cgctttgcgt ataaattccc ttaacagaag
    27421 cttcgatgtg gtgaaatagt tcatgtccaa taatcaaagc ttgcgctgtt tctttagaga
    27481 tcgaagctag tcctgaacat tttaattttt ctaacggctc cgtcatcaat cgaatccctt
    27541 catcttctgc ataagttgca aaagtgattc gttcgtttat ttgctctgat tcacaggaaa
    27601 cgattggaac agccatttgt tcacaaattt ctcctacatc ggcaaaaggg tacttttttt
    27661 tgatgctaca tgccgtttcg ataccacaat cgattgattg ttgtataaga attttctgct
    27721 tggacaaagg aatctttttc caaaataaat ctcgccgaaa ctggtagaat ccccaaattt
    27781 cttcggggat ctgttgtaat tcgtttagta actcttctaa cttatgtttg tttgatattt
    27841 ccggcaacga cgatcacttc ttcctccggt tccatagtag atacttccag atccatcaaa
    27901 agctgtagtt gttccagatc cgtcgctgta aaatcagaga ctcttgctcc taagcagaga
    27961 taaaattctg gtcgggcaat caattcagtt tgataaactg ccatatcttc ccaaagttgt
    28021 tcgacagctg tcatataatt ggctgcagtt gttttcatac atgaagcatg tccacgttgg
    28081 accttgataa atcctttttg atcgatgatc cgaagacttc cagcattttt tgaacgattc
    28141 tgttcgccgc atacataaaa gaaaggtgta ttttccaata agcaaacttc atttttattt
    28201 aatcgcatat cctctgcagc aagctctagt gcttcttctg ttgtgatctc cttggttaga
    28261 tcggttgctt tcacttccgt agaaccagta gcgatagccg ttacttttga agtctgtgga
    28321 tcgatttcta cgtggacttc gatcgattct ggtgtagcgc ctgattcaat cgctttattc
    28381 atcgcttcat ttttcaacga tcgtatatct tctttacttg gcgaagggat gatacgttct
    28441 acaacatctc ggaccattgc aagtgctaca ccaatagaag aaatcacttc ggcgttttca
    28501 ggaatcgaat atttcacacc cattttatta ctaaagtaag taatcaatga ggcagcgccg
    28561 ccccctacac ctacaagaga aatctgatct ttctccaagt gatacttgtc tgccaattct
    28621 aaaatcaccg gttcaatctt agcataggat ttttccatga tctgttctgc gatgtcttca
    28681 accgtcgtat gacaataatc agctaatgct tggatggctt ttcgtgcaga tgggacattt
    28741 ccataagaaa aatgttcttc ttgcactaat ccaaggacat tggctgcaca tgtatttgta
    28801 atagtcactt cttctccgtc ttccatcact accttgacgt aatcagcagg gtctccttct
    28861 ttaggagaga aaaattcaac cttaggacct ttgatttttt ctgtctccgt aaatactgca
    28921 taatctagac cggcaatatg tgcagaacgt ggaccgacat cgatgattcc tgactgatta
    28981 gcccgtacca tcgaaccgcc tgccacacct agaacccgaa catctaatga agagatatat
    29041 gttgggtggc cacctacaat tgaataatcg attgctggac gtccattttt aatgaccccg
    29101 atattggttg ttgttccgcc tacttcaaaa taaacaccat tagacgctct caagtacatc
    29161 aaagagccca tgactgatgc tgctggaccg gaaagcatgg ttaaaacagg tcgttttttc
    29221 atttcattga tttccattac tccgccgtct ccgcgcataa tcatcaaaga aacattgacc
    29281 cctgcttctc gtacggaatc ttctgtagag gttgccgtat ctagcatttt tggtaaaata
    29341 gatgcattga ttgctgctgt ccgagttctt cttgtcaatc catataattt tgtgatgtca
    29401 gaagccatcg ttgtagggat cgattttacc gatgctgctt cataaacaac tcgttctggt
    29461 tccccattat ctactccaaa tgccatagaa gaaaccaaga cctccgctct ttctctttct
    29521 aaagaagaaa tcgtttctga aacacggtct acatttaagt gtttgacagg taagaatgca
    29581 ttgacaatct ctattttttt cttattccct aaatcgatat cgttcaatct agtttgtcgt
    29641 tttgctaaaa aaccttctaa tccacctttt gccatcccga ttacgccgac ttttgctacg
    29701 tctccttcaa tcagtgcatt agtcgcttgt gtggtagaat gtgcaacaaa cacaacatct
    29761 tctggactga tattattttc cagcaaacaa ttttgaaaag actgaacgac acctgcggct
    29821 actccacgga catcatcgtg ggttgttttc actgaagatt ttccgatgat ttcgtgcgtc
    29881 gcattatcga ttgcgactgc tttcgtatgt gtaccgccaa catcaattcc cattcgcaca
    29941 aaacgtttca ttttcttttc ctccattcat ctaatcgttt ttatcgacgt tttgtttact
    30001 aggcgttccc gtacaataag aacgtgatca ggcataagat cgctccgatg atccaaccag
    30061 ttgggatgga tttcttcata tattctcgag aagttacttt tgtataacct aatccccaag
    30121 caacccaaga ttgtgtaata tctaaatgct gaggagcaat cgttgtgatt gcaaataacg
    30181 ggaacaaaaa ggctggcgac atcgttggat taacagctaa tacgacagct aaaatagctg
    30241 agcctgaacc gactagattc attggaccgc ggaaaaatcc tagtggtgtc aatatagcaa
    30301 atacgattgc caagactaat ggtgattgcg gtatccaatc tccaaaaata gctgaaaaat
    30361 aaggagaagc atacgcagct gaattattga acatcgctaa ggtcatcaag aaaccaacca
    30421 ttggcgcaac atctactgcg ccatctgtaa ataatttcgc aaactgacga cagatttctt
    30481 tataacctcc gcgcaatttt ccagtaaaca gcaatgccca gatacctgct agaataaaac
    30541 ctaagatgat tggaagatct aaaagaacga cccctaaaac tggtaataat acggctaacc
    30601 atgagatcat tggtgcgtcg ccaccttcag ctggtacgtt tgctgccatt gcatagcgtt
    30661 tctttttgtt cattgatata ttagcgaccg ttagtaccac aactaagctt acaatcatcc
    30721 caatcatacc aatttggaaa tagtcattat aagtatagct ttcagccgct ggattgaaac
    30781 cagcataaat cgtttgatat tgtttgaaat tgacgatatt agcaaagatt ccagccataa
    30841 tcgagcccat aaatgagaac agtgcaaccg gtactgaaat acctaatgac atcaagattg
    30901 gcaacacaat aactgcaata gaaatcactg gtccgattcc tgtcatcgac atgaatataa
    30961 cagcggttac aatacatagt aagctcatcg tgattcttgg cttgtcccct ccaagttcaa
    31021 ctacctttcg aatcagggta gctgcgattc cgctatctac tagaacacgc ccgaaaaagg
    31081 caccaaagaa gacatttacc aagatcgatt tggcgtactc agctggcccc gtcgtataga
    31141 tatgcgtcaa cacatcaatt actccttgcc cttccatcgc cgggtttggt gaaaacgtgt
    31201 ttccgactag cgctaatccc atccatacag tagccatgat ggcaaagccg accatcaagt
    31261 tgtagccttt tacacagtac cagaccatga aaaagaatga gataactaac aaaataccaa
    31321 taataatatc actcatgtta ttcctccgtc cattttgttt ttctgataag caaactaata
    31381 tatcagtcaa actcattcta acttatgtag ttagcgcttt cattaaaggg tgaccataaa
    31441 tgttcagttt tcaattaaaa aaccttaatt atgctatttt agacttatac gttattgtcc
    31501 tgtttcattt gatggaggat tgtaaataaa aaaagaggaa agaacataac ttttcgttat
    31561 cttctttcct ctttataatt gtttagctta tttagccaaa gcttcttttg cttggtcagc
    31621 caatgcagta aatgctgctg catcgttaac agccaagtca gctaacattt tacggttgat
    31681 gtcgatttca gcaactttca aaccgtgcat taattttgag tagctcaagc cattcatacg
    31741 agctgctgcg ttgatacgag caatccacaa tttacggaag tcgcgtttct tttgacgacg
    31801 atcgcgatat gcgtagttgt atgaattcat tacttgttct tttgctgttt taaataaagt
    31861 gtgttttgaa ccgtagtaac cttttgctaa ttttagcact cttttacgac gtttacgagt
    31921 tactgttcca cctttaacac gtgccatgta taattcctcc taataaaaat aaatcttcag
    31981 attctgttat ttgtacttct tatttcattt ttgctagttg ttggcgaata cgtttgaagt
    32041 cgccactaga aaccatgctt gctttacgca attgacggcg ttgttttttt gttttgccgt
    32101 ggaaacggtg acttgtaaac gcgcggaagc gtttcaatcc gcctttacca gtacgtttga
    32161 aacgttttgc tgatccgcgg tgtgtttttt gttttggcat gactatattt cctcctcaaa
    32221 atctttcgtt atccgactat cggctgtcca acagccagtt agtcattcaa attacttact
    32281 gtcgtttttc ggtgccagcg tcaagaacat gctgcgtccg tccattttcg ctttttgttc
    32341 cactgttgca atatccgcag tttcttcagc taagcgattc aagacttttt gaccaatttc
    32401 tttatgggta atggcacggc ctttgaaacg gatagaagct ttcactttat cgcccttttc
    32461 caagaactta cgtgcattac gaagttttgt attgaagtca ttcaagtcga tcgtaggact
    32521 taagcgaact tcttttacat tgatcacttt ttgtttttta cgcgcttcgc ggtctttctt
    32581 ttgttgttcg aaacggtatt taccgtagtc catgattcgc gcaactggtg gtttcgcacc
    32641 aggggcaact aacacaagat ccaagtttgc ttgttctgca atattcaatg cttctgcttt
    32701 tgtcttaact cctaattgct caccatctga accgatcaat cgtaactctc gtgcacgaat
    32761 gccgtcgtta accatcatat cctttgctat ggtcattcac ctccaaatat tatgagagaa
    32821 agaaaagctt accgaataaa cggcaataaa aaaacgagtt ctcttacaga acccgcgcgt
    32881 acttcaccta atagaaccag tgaaaactat ctagcccagt gacattatca actaaggcga
    32941 gaagcgggtg cttctgcttt tatttctcaa ccatcatagt atagcaaaaa ctaatctttt
    33001 tgtcaatgtt tttcatacag tttgtcttcc ttttctgaaa atgattagat tagcagactt
    33061 tctagcagtg attcccgctt ctttgctata ctaaagaaaa acgaggtgaa cccacatgaa
    33121 aaaaatcgaa cctattccac cttctcttcc tcttcttaca aagggagagt tttatttaga
    33181 agatgaggag atcttctctg gtcttctgat caacgggggt gaccaaagct atctcgatgc
    33241 caaaaatatg gtattaagag agagtcatat ccaaaaactt acgatgcaaa aaacagtttt
    33301 acgtcatttt gaatgcagta atgtcatttt tgaaaaatgt gatttttcca atctggagtg
    33361 gattggtggc agtttccacc aagtcgtgtt tcatcaatgt aaattaacag gtacgaattt
    33421 tgccgaaagt tatcttcgag actgtacttt cactgattgt attgcttcga tggcttcctt
    33481 tagtagtaca aacttgaaag ctgtctcctt cgatcattgt caattagaag acagcgaatt
    33541 ttatgaggtc acttggaaaa accttttttt gtctgacaat cagttgaccg gttcgaactg
    33601 gtttcgaact tctctgaagg gattagactt tacgactaat ttcttcacag caatcgcttt
    33661 atctcaagat tctttggcgg gactaatcgt caaccaagag caagcacttg tgatcgccca
    33721 agcccttggt ttgcagatca aagactagat caacgaatcc gccgccacag tgcaataagc
    33781 atccaaatag ataagaccaa actgatgatc aaggcgattc cccatccgaa aacggaatgg
    33841 gtaaagggta actggagatt cattccgaag aatcctgtca caatagttgg aacggtcaag
    33901 atcagcgacc agaccgtcaa aaatttcatc gtgtcattca agttgttatt cagcaaatta
    33961 ttataagtgc cagagagctg gtctaaaatc tgcgaagcta agttcgtcat ttcaactgct
    34021 tgtcgtgctt cgatcagcgc atcgtccaac tcttcttttt ccttttctga caacttgcga
    34081 taaatagcca atgccttgat ttgttctaaa agtactacat tttgttttgt tcctgtaact
    34141 aagtaaacca aaccgatctc taaatctgaa agttgcagca gattcttgtt cgttgttttt
    34201 tctcttaatt tcagattaag ccgctgacgt tcactattca cttcctcgat taaaggaaaa
    34261 aaggaatcag aaatcaaaaa caatgtacga aacaataaac tgtatacact catgtctggc
    34321 attcgttcca aaagcgatcg aattagtcga atcacgtact cggtattcgg cgtggtaaat
    34381 gtaaacaaac cgtcttcttt taaaataaaa gcgataggac tagtttcata atgattatcg
    34441 actttttgct ggtgcggaac atttgataca agcagtaacg cttcatcaaa agaatcgtac
    34501 tctacgcgcg cccgttcatt tttatctaga gaataagtca gcatctcgtc actaatgcca
    34561 tattcttctt ttaagttttt aacagctagc acatcgtttt gctgtacatt gatccaaaaa
    34621 ttacggtctt gattaaaata gtgtttttcc cgcatactct atcctcctga catttgtttc
    34681 cagtataaag aaaaaagtcg ctcaaataaa ggtttaccct ttatttgagc aactgtaaat
    34741 caaatggcta ctttggtttg gcgaatctct gctaattttt cttcgatcat tttcaagact
    34801 tcttcttttg cttgaggatc ttccacaaaa ttatagcggt ctccatcgat ttggattttt
    34861 ggactttctt tgtattcttc ataccattga tcgtaacgtc tgttcaattc tttgtaataa
    34921 tcgtatagac ttggatcaaa ggacaattgt tcatacgaac gaccacgttt ttcgatacgc
    34981 tccaacatcg tatcaaaaga tacacgaata tgaactaata aatccggatg ttttttatgg
    35041 gcagcatagg gaagttcttc catcatgttt tccagcagtt catcatacac acgaacttct
    35101 gtttctgttg ctcttcctag atctgcattt agatggaaaa gcagtgaatc ttcataaatc
    35161 gaacggtcca atacattatt gtcatctttc attgcttgtt ttatgcttgc aaaacgttta
    35221 ttcaaaaaat aaatttgcag caaaaaagca tactgttcag ggtttgcata aaataagggc
    35281 aagacttcat tatcatcaat cgattcataa aatgcctgac tatcaaagtg ttcagccatc
    35341 atttctgtta aacttgattt tccggcacca attgtacctg ctaaaacgat cacgctcatt
    35401 tacctccagc tcacttcatc actaaatatt gtgtttaact ccaacctttt taaatatacc
    35461 cccaatatct agtagtgtca attgtatttt tttctagtca aagaagaaaa aaagtcaaaa
    35521 aattcttaag aaaaaagtgt tttttcttta gaaaacgtat tcttagtttt acttctgaga
    35581 tggttttagt aaaataaagg aggaatgttt tttgagaccc ttagtggaca aaaaaattcg
    35641 tgaagcagcg caactcgttt tgggcgcttt attttttgct taaaaatcac ttttacgtga
    35701 gaaggagtta gattatggca aactatggcc aaggagaatc aagcggaaag atcatattga
    35761 tgggcgagca cgcggttgtt tatggagaac cagcgattgc ctttcctttc tatgcaacaa
    35821 aagtcaccgc attccttgaa gagctggatg caatggacga tcaactggtt tcttcctact
    35881 attcaggaaa tttagccgaa gctcctcatg cattaaaaaa tatcaaaaaa ttattcattc
    35941 acttaaaaaa acagcatgac atccaaaaaa acttgcaact gaccattgaa agcacgattc
    36001 ctgctgaacg tggaatggga tcaagcgctg cagtcgccac agcagtcact cgtgcttttt
    36061 atgattactt agcatttcct ttgtctcgtg aaatactatt agaaaatgtc cagctttcgg
    36121 aaaaaatcgc ccacggtaat cctagtggaa tcgatgcagc cgctactagc agcttgcagc
    36181 cgatttattt tacaaaaggg catcctttcg actacttttc tttgaacatc gatgcttttt
    36241 tgattgtcgc tgatacagga atcaaaggac aaacaagaga agccgtcaaa gatgtcgctc
    36301 acctctttga aacccagcct catgaaactg gacaaatgat ccaaaaatta ggatacttga
    36361 cgaagcaagc aaaacaagca atcattgaaa attcaccaga aacgttggca cagacaatgg
    36421 atgaatcaca atcacttctg gaaaagctga caatcagcaa tgattttctt aatctattga
    36481 tccaaacagc aaaagatacc ggggcattgg gtgccaaatt aactggcggt ggacgcggtg
    36541 gctgtatgat tgcattagca caaacaaaaa caaaagccca agaaatcagt caagcacttg
    36601 aagatgcggg tgctgctgaa acttggatac aaggattagg agtacatacc tatgtttaaa
    36661 ggcaaagcac gcgcatatac caacattgct ctaatcaaat attggggtaa gaaaaatgaa
    36721 gaactcatcc ttccaatgaa caacagcctt tcgttaacat tggatgcatt ttatacagag
    36781 acagaagtca tcttttctga tagctatatg gtagatgaat tttatctaga tggcaccttg
    36841 caagacgaaa aagcaacaaa aaaagtcagt cagtttcttg acctttttcg taaagaagcc
    36901 ggcctctcac taaaagcttc agtaatcagt cagaatttcg ttcctaccgc agctggatta
    36961 gcctcctctg ccagcgggct agctgcttta gcaggcgctt gcaatactgc tcttaagctt
    37021 ggattagatg atctctctct ttcaagattt gctcgacgcg ggtctggttc agcttgccga
    37081 agtattttcg gtggtttcgt cgaatgggaa aaaggccatg acgacttaag ttcttacgct
    37141 aagccagtcc cttccgattc tttcgaagac gatttagcaa tggttttcgt tttgatcaac
    37201 gaccagaaaa aagaagtgtc cagcagaaat gggatgcgtc ggacagtcga aacatccagt
    37261 ttttatcaag gctggttaga ttccgttgaa ggggatctat atcaattgaa acaagcaatc
    37321 aaaacaaaag atttccaact tctcggagaa acgatggaaa gaaacggact aaaaatgcat
    37381 ggaacgactt tagctgccca gcctccattc acttactggt ctcctgacag cttaaaagcg
    37441 atggatgcgg tgcgacaatt gagaaagcaa ggcattccat gctactttac gatggatgct
    37501 ggccctaatg tcaaagtttt agtggaaaac agccatttat ctgaggtaca agaaacattt
    37561 accaaattat ttagcaaaga acaagtaatt actgcacatg ctggtccagg aattgcgatt
    37621 attgaataaa gggagatttt tatgattgaa gtatctgcac caggcaaact ctatattgcc
    37681 ggagaatatg cagttgttga aacaggccat ccagcagtta tcgctgcagt cgatcaattc
    37741 gtgacagtca ctgtagaatc tgcacgaaaa gtcggaagta tccaatctgc acaatatagt
    37801 gggatgcctg tacgttggac aagacgcaac ggagaattag ttttagatat tcgggaaaat
    37861 ccttttcatt atatccttgc tgctattcgc ttgactgaaa agtatgcaca agaaaaaaac
    37921 attcttttat ccttttatga tctgaaagta acaagtgagt tagacagttc aaatggccgg
    37981 aaatatggat tgggttctag cggtgccgtg accgttgcaa cggtcaaagc cttgaatgtt
    38041 ttttacgcgt tgaatttatc tcagttggag attttcaaga ttgcagcact agccaattta
    38101 gcagttcaag ataatggttc ctgcggcgac atcgctgcta gctgttatgg tggctggatc
    38161 gctttctcaa ccttcgacca tccttggctc caagaacaag aaactcagca ttctatcagt
    38221 gagttacttg ccctggattg gccaggtcta tccattgagc cattgattgc tcctgaagat
    38281 ttacgtttat tgattggttg gacgggtagc cctgcctcta cttctgattt ggtcgatcaa
    38341 gttcaccgtt cgagagaaga taaaatggtg gcttatcagc ttttcttaaa aaatagtaca
    38401 gaatgtgtca acgaaatgat caaagggttt aaagaaaata atgtaacgtt gatccaacag
    38461 atgattcgaa aaaaccgaca attactgcat gatttatctg caatcactgg ggtcgtcatc
    38521 gaaacgcctg ctttgaacaa attgtgtaat ttagctgaac agtatgaagg agccgcaaaa
    38581 tcttctggtg caggtggggg cgattgcgga atcgtaattg ttgaccagaa atctggcatt
    38641 cttcctttaa tgagtgcatg ggaaaaagca gaaatcactc cactgccgtt acatgtctat
    38701 agcgatcaaa gaaaggaaaa ccgatgaatc gaaaagacga acatgtttca ctagctaaag
    38761 cctttcatga taaacaaaaa aacgaatttg acttcgtacg gatcatccat aaccctttgc
    38821 cgcaaatagc agtttctgat gtcgatctaa gtacacaggc agttgggttt acactaagca
    38881 gcccatttta tatcaatgcg atgactggcg gaagcgaaaa aacaaaaaaa atcaaccaag
    38941 atcttgcaat cgtcgcaaga gaagcagatt tgatgattgc cactggttca gtcagcgctg
    39001 ccttgaaagt cccttcttta gccgatactt atacgattat gcgacaagaa tatcctcacg
    39061 ggaaaatcat tgccaacatc ggtgcaggga cttctgtcga aagagccaaa gaagctatcc
    39121 gattgtttca cgcagatgcc ttacagattc atttgaatgc acctcaagag ttggttatgc
    39181 ctgagggaga ccgtgatttt accaattgga aagtacttat tcaagaaact caaacagcca
    39241 ttgacgtccc cctcatcgta aaagaagtcg gttttggcat gacaagagaa acgctcaatg
    39301 atctagctgc tttaggagtt catacagttg atatcagcgg tcgaagcgga acaagcttca
    39361 cccagatcga aaacgcccgt cgctccaagc gggaactgag ttatttagct gactgggggc
    39421 aatcgacagt ctcttcattg cttgaagcaa atgaagcaga cacttctatg gaaatcctag
    39481 cctccggagg tatccgcaat gcctatgaca tatttaaagc cctttgtctc ggcgcaaatg
    39541 ccgtgggtac ttcaggaacc gtactgacgc atctgatgaa tcacggcgta gaagaaacta
    39601 ttatactgat gaagcaatgg caagaagagc ttcgtctgct ttatactatg gtcggcgcaa
    39661 cgaataccgc ggccctacac caacaatctc tgatcttttc cggaccagtg aaggattggt
    39721 gcgaagcaag agggatcgac ttagtgaagt atggaagacg cacagaaaag agcgtgggac
    39781 aaaagtaaag aacacttttg tctcacgctc taaacacgta caaacggcgg aagtaaaagc
    39841 aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaattcc
    39901 ttcttatttc ttggagttaa atgcttctgt cccagcctct tttttcgaat aaatgatgtt
    39961 taatcagctg tccttcggta ttcgttcaat ccgctgattt cacaacctct ccactaaaca
    40021 gtgtactaaa gctcttttaa ggttgttttc cgatataggc aagtatgcct ccgtccacat
    40081 acaagacatg gccgttaacg aaatcagatg cgctagaagc caagaagaca gatggtccag
    40141 ccaaatcgat tggctcgccc cagcgtgctg ctggcgtacg tccaacgata aattgatcga
    40201 atggatgacg ttcaccgttt tcttgtgttt cgcgtaatgg agcagtttgc ggtgtagcaa
    40261 tataacctgg tccaattcca ttacattgga tattgtattg accgtattca gaagcgatgt
    40321 ttttcgtcag catttttaat ccgccttttg ccgcagcata agcactgacc gtttcgcgtc
    40381 ctaattcact catcatggag cagatattga tgattttacc gccgcctttt tcgatcatat
    40441 ccggaataac tgcttttgaa acgatgaatg gggcatttaa atccacgtcg attacttggc
    40501 ggaagtctgc cgcatccatt tcgatcatcg gtgtccgttt gatgatccct gcattgttga
    40561 ctagaatatc aactgaacct acatcttctt tgatctgttg aaccatcgct tgaaccgctt
    40621 cttcatcggt cacatcgcag acataacctt ttgtatcgat gcctgcttct ttataatgcg
    40681 ctataccttc atcaactgat tcttgattca agttattaaa tacgattttt gctcctgctg
    40741 tagctaaaga gcgtgcaatc tcaaatccaa taccatagac agcaccggtg actaatgcta
    40801 cttttccatc taaacgaaac atatccatac taaattccat cattttctct cccttatgct
    40861 tattttaatt gatccatttg caccatatcc atgtctgtat aggtgatatt ttctccgcac
    40921 attgcccaga tgaatgagta attgcttgta cctacacctg aatggatcga ccagcttggc
    40981 gaaataatgg cttgttcatt gtctacaact aaatgttttg tttcatctgg tttgcccatc
    41041 atgtggaaca cacgtgtatc ttcattggca taatcaaagt agacataagc ttccatacga
    41101 cgttcatgtg tatgacaagg catcgtattc caagcgcttc ctggttctaa aatcgtataa
    41161 cccatttgta attgacagct ttcgcataca tttggatgga tgtattggta gattttacgt
    41221 tcattcagtg tcaggccttc accagtctcc attggtttta cttggtcgat actgattttt
    41281 acatttggat atttatgatg agctggaaca gaactaatat agaatttagc tgggttttca
    41341 gcatcttgag aagaaaatac aacatgtcgt gtttcttttc cgatatagta tccgtcttgt
    41401 tttttcattt cttctttttg cccatcgatc tcgatgaatc ctggtccacc gatattgatc
    41461 acacctagtt cacgacgctc taagaagtaa tcaacaccta gttctttgtc taatgtgatt
    41521 tctaattctt tttcagtagg tgtcactcca ccaaagatca ttcgatcgtt gtgcgtataa
    41581 gtcaaactga ttttccctgg ttcaaatacc ttttctacta aaaattcttt tctcaattgt
    41641 tccgttgaat aatgacggat atcttctgga ctgtgtgtat aacgtgtttc catttcttgc
    41701 attttcttct actccttcta tcgaatgatt tttccagagc cgcatgctaa aaattgtgcg
    41761 acttcttctt gactaaattg gttgcagtcc ccatggatcg tatgcttcaa ggcagaagct
    41821 gccgtagcaa aatcaatgat ttcttgcgga gacatattgg ataaaatccc atggagcacg
    41881 ccacctgcaa aagcatctcc gccacctact cgatccacga ttggggtgat tctatggact
    41941 tgggattcca catattcatg gttcatccac aatgtcccct tcaattcatt ttcacttgct
    42001 gaatgaacgg tacgcttggt cgaataaaac acctgaatat tcgggaactt ttcctgcatt
    42061 ttttgataat aataaattag aggttgttct tctgcttctg gcgctttctc gatacctaac
    42121 aaataaagag catccatttt tccagctgag cagatatcta ctaaaggtaa aagctgatgg
    42181 attacttcgc tggcttcttg ctgtgtccat aattttccac gatagttgat atcgaagctg
    42241 atcaggcagc cagctttttt tgcagcttct atcacctgtt tcgtcatttt ttgccatttt
    42301 ttagacaaag ctggggtgat tcctgaaaca tgaaatatat ctattccttg gaataactcc
    42361 tctagcgacc attccaaatg atccatttca gcaaaacttg atccggcacg atcatagatc
    42421 actgaggcgg cgcgttcgcc actaccagtt tccatataat aagtacctag tcttgatcca
    42481 ccagataata cctgtttcgt atctactcca taacattgca agtgcttttt gactgctttt
    42541 cccaatgcgt tatcaggtac ttttgtagca aaaatcgctt catgtccgta attagctaaa
    42601 gaaattgcta cgtttgcttc ccctccgcca taatgggcag cgaatcgttc acttgaatcg
    42661 atacgggttc cctgctctgt ggacaatcgc aacatgattt ctccgagtgt cactactttt
    42721 cccataagca gctacctctt gatttctttc atttttgctg catactgttg cgcgacttcc
    42781 gttactttgt caaaatctcc tgtagctgcg ggtgctaaaa ggttcccacc tactccgact
    42841 gttacgacac cagcatcgaa ccactctttc atattgtcca gactcacacc gccagttggc
    42901 atgatattga cttgaggcat cggcgccttg aaggcagaaa tgatacttgg tccataagcg
    42961 cttccaggaa atagtttgac gatatctacc ccgcttttca gcgctgtttt gatctcggta
    43021 atagtcatac atcctggtaa gtaaggaatt tgatacagat tgcacatctc tgccgtttct
    43081 tgatcaaagg aagggctaac aatatactct gcgccagcta agatagccaa gcgagctgtg
    43141 acagcgtcta atactgtgcc tgcgccaatc actatttctg gttggtcttt gtagaatgac
    43201 aaaagttctt tgattacttg atctgcttga gggactgtaa aagtcaactc aataccagtt
    43261 agaccacctt tgatgattgc gtggcaagca ttcaaagctt cttctttgct ttttccgcgt
    43321 accaccgcaa tcactccggc gtttttcaat cgagataaaa tatcaatttt tttcatcttc
    43381 tccacctatt tcctatttag aaattttatt ttataacttg gaaatctata cccatattat
    43441 acggcagaaa agaaaaattg caaacgcttt tttcattatt tttgaattct ttgctcgatt
    43501 tcgctttttg tttgcttgag ggcactgatt atttgttctt ttgtctcatc tgttaaacga
    43561 tatttcggca tacttacact aaatgcacca atgatttgtt cgtttttcac gattgaggct
    43621 cctatacaaa aaatatcacg ttcgatttct tcatcgtcaa atgcgatgtg ggtttctttt
    43681 accgttttga tttccttttt caaccgtaat gcattggtga tcgtatattc tgtgtaaggg
    43741 atcaacggat gcttttcaag ataatcagtg tactgctctt cactgaattc tgctaatacg
    43801 gctttgccca tcgcagaatt gtataatggt cgggtgattc cgatttttga agacatgcgg
    43861 atcgtttgat gttttggatc tagtttgttc acgtacaaga tctcattgtt gtccaatacc
    43921 cctaagtgga tagtctcatc gatcttttgc tgcagctgtt ctaaaaacgg taaagtcaac
    43981 tctaccaaat cgatttgttc aatgttttta ttcgcgtagc gaataagctt tgcacctaaa
    44041 cgatagtttt tctctgaatt tttatttacg tagccgatca tcaacaaagt atctagtatt
    44101 ttcaagactg tagaagaggt cattcccaca ccctttgcga tcgtctgcaa agagacatct
    44161 tggttttccg ctaaatagtc tagtatttta gaggctttga tcaatacagt tccataaggc
    44221 tttttatttt ccatataata ttctcctatt tctttataag aaaaattata gcggaaatac
    44281 catcaattta aaagagatat ccagatataa actaccattt ctctttacag gcaatttaat
    44341 gttgaagatc cgcttcgttt ttacatcaaa ctttgatata aagcaatgat ttcttctact
    44401 gtcgtttctc ttggatttcc tggtgtgcat acatctcgca aagcatcttc tgcaattgct
    44461 ggaatatctg cttcttttac acctaattca gaaatggttt ctgggattcc aaccgctttg
    44521 ctcaaccgat cgatctcacc acaagccgct tctcttacat cttctaaaga catttcagct
    44581 gctcctttaa tccctagaac aagagcgatc tcacggtact tttctccagt atattctttg
    44641 ttatatttca tgacagttgg caaaagtgct gcacacgcta cgccatgagg aatgttgtac
    44701 catgcactta acgggtgagc catcccgtgt accagtccta aaccgacatt tgagaagccc
    44761 attcccgcaa tatactgtcc taatgccatt ttttctcgcc cattttgatc gccatctaca
    44821 gaagcttcca aagaatggcc gatgatttcg attgctttta aatgcagcat atcagtcatt
    44881 tcccaagcgc cttttgtgat aaacccttca atagcatgcg tcatagcatc catacctgtt
    44941 gatgcagcca attttttcgg cattcccatc atcatatcgc tgtcgatgaa cgcgacgatt
    45001 gggatatcat gaggatcaac acaaacaaat tttcggtgat ttacttcatc tgtgatgacg
    45061 tagttgatcg ttacttctgc tgctgttcca gaagtcgttg gtatagctaa tataggtaaa
    45121 catggatttt tcgtatcagc aacaccttct aaactaatga cgtctgaaaa ctctggattc
    45181 gtcaaaataa tcccaatggc ttttgccgta tcgattggcg aacctccacc gattgcgatc
    45241 aaacagtctg cctctgcctc tttaacaaaa gccactccat cttttacatt ttggatcgtt
    45301 ggattaggta cgatcccgtc gtataaagca taagcgattg ctgctttatc tagaagttct
    45361 gttacttttg ttgctatacc gtgctcgatc aatcctttgt cagtaatgac agctgctttg
    45421 ccgaatcctc gctgctgaaa ttcagggacg atcgactcaa tcgctccttt tccaaaatac
    45481 gatgtttcat ttaaaatcat tcgattactc attttctatt cctcctaatt tgtattattt
    45541 ttcacaataa aaacgaaaga accccaagca accggttgcc cagttgcttg ttgctacagt
    45601 tcaaagactg gcatcaattc ccaactgact ggagaggcat ctggatttgt ctccatgata
    45661 tcttccatgt acttccacca tttttgattg atagatgtat ttgccatttt ttcccataac
    45721 ttttcatctt cgatcactaa gtaaccaaat aacaaacttg tactcggatc aagatagatc
    45781 gaatagtcga tgaccccgtg ttcacgaagt gctgtacgca tttctggcca aagttcatca
    45841 tgtctttttt tgtattcttc ttgtttttct gggtaaactt tcatgcaaaa agcttttttc
    45901 accatgtcag acacctgctt tcgtatgaac ttcctccaaa taacctgctt ttggagtgac
    45961 accaaacgca tctgctaaat gccacaggtt ttcgtccgtg attgtttgca agattggtcc
    46021 ttgcgctttg acgtacgtat agacttctgc tgccttttct gctgtctcga tcaaaccaaa
    46081 gacttcatcc atgtcttttc ctgacccgta gattccatgt tgtggccata aaaccaatcg
    46141 agtttctttc attttttcag ctgttgcttc cccaatctca tttgttcccg gaaccatcca
    46201 cggaatgatg gaaattcctt ctggaaaaac aactaagcat tccgtacaca tttgccatag
    46261 cgtacgacta aaggaacgtt cgtctaattc atgcgtaaaa ctcatcgcta atagatgact
    46321 agcatggcaa tgcataacga tccgattatc tgggtctact tctaaacggg cgatatgact
    46381 catgaaatga cttggcaact cgcttgtagg aaggcattcg ttttccagcc cccacaaaag
    46441 ttccagtgtt ttgccatctt cagcaactcg aacgattcca agattctcgg caggttcgct
    46501 cgctacattt ttaaaatatc ttcctgaacc agttacaata aaatagcatc ctgctaaacg
    46561 gctggcatca aaaatcatcg agatgcggcg gatcactttt tcaggatcca agaacggtgt
    46621 gatctcttct tcttttagta aataagaaat attcccgcca tttctttcat cccatcccaa
    46681 gcgataaaga ttcgacgtcg tttcgatcat ttctttcaca taaggcgctt gtaaaatatc
    46741 tatttttttc aatatagcca cactcctatt ttcttaattc caacacgtct ttttgatatt
    46801 cctggacttc ttccagccaa tccaaaccaa ctggaacttg atttttgtaa cagaaataat
    46861 tccatacatc ggcatacggg aaatctttca attcttccgt ataagccatt cttgaagtaa
    46921 agtcaaaagc cagctccatt tcttccaatc gctcaactgg ttccagcata gctttcagca
    46981 gtgctttttg tgtatttctc gtaccgatga cccaagcagc gatacggttg atcgttgcat
    47041 caaagaaatc cagaccgata tttgtttttt ccaacaattc gttacggact aattctttag
    47101 cgatttcctg caattcatca tccatcaaaa ccacatggtc tgaatcccaa cgcactggac
    47161 gggaaacatg caacatgatt cctttgctga ataaagctaa tgaagataat ttattggaaa
    47221 tcacttctgt tggatgaaaa tgtccagcat ctagacaaac aagtttgtca cgagtcaacc
    47281 cgtaacccat ataaaattca tgggaaccga ctgtataagc ttcagcgcct aaaccaaata
    47341 gtttgctttc tactgcatct tgtacatacc gttcatctaa ctcttctgta aaaatttcat
    47401 cgagtgattc cattaaacgt tcccgtgggg taaaacgatc aatcggatta tctttcatcc
    47461 catcaggtac ccaaaagtta ttgatacttg tttgcccagt ttctttccca aagtactccg
    47521 cgattttgcg gcttcttttc ccgtgttcga tccagtattc acgaacttct ttatctggat
    47581 gagccaatgt aaagccgtct ttcatcattg gatgcgaaaa gaaagtcgga ttgaagtcaa
    47641 gaccgatttc ttcttttttt gcccactcta cccatttttc aaaatgtttc ggctcaattt
    47701 catttagatc gacagattct tctgtatcca aatagatagc atgaaggttc aatttgtgtt
    47761 tgcccggtat caacgaataa gccttctcca gatcttgtct aagctcttca ggtgttcgtg
    47821 cggctcctgg aaaattccct gtcgccatga ttccaccggt caattcgcca tccggtgtca
    47881 agaaaccttt gacatcatct ccttgccagc aatgcataga aattttgatg tcctgtatct
    47941 tttctagtac agcatctgta tctacaccga tagttgcata tttttctttt gcttgttcgt
    48001 aatttctttc aatcgtgttc atacgattac tcctttgatt ttattcacgt accattccat
    48061 cgtgttttgc ttattttcat ctgggtaaaa agtttcacat ggaaacgact gtttgattac
    48121 atttcttgct tgttctatat tcgaaaactg acctgtactg agcatttgca tggcaacatt
    48181 cccgattgct gtggcttcac taggtcctgc ttctacaatg attccggcat aatctgctgt
    48241 caattgattc agtagacgat tattcgatcc gccccctacg atgtgcagct tttccaattt
    48301 cgttccagtt aagtgttcta ctttgatcag ctcttgtgcg taacaaagag caagattatc
    48361 atacacacaa cgagcgatag ctcccgatga gacagggact ggctgctctg tttcaaaaca
    48421 gtattgctga atctcttcta tcatatttgg gggattcaaa aatcgagggt cattgatttc
    48481 catcacttgc tgcagcggtt tttcttttgc agcaagctct gccaattccg cataactgta
    48541 ctcgtactct tgcattcggg caacttcctg gatcaaccac atccccatga tgtttttcaa
    48601 aaaacgcacc gtgttatggg caccccattc attcgtataa ttttcagaaa aagcattgcg
    48661 agaaacattg gcgatctttg tttcaatccc taaaagcgac catgtcccac tgctgatata
    48721 accccaacgt tctccttgac ctggcgttcc taatacagct gacgccgtat catgacttgc
    48781 tactgtgata aaggtcacat ctggcaaatc atattttgaa aattgttctt tttgcagatc
    48841 tcctagtatc gttcccggct ctgtcaatgg tggaaataat tccttcgaga caccgatttt
    48901 ttcaagaagt tctatttccc attcttttgt ccctgcattc agcagctgca tagtagatgc
    48961 attagttttc tccgtcacca tgttaccagt aaagacatac cctaaataat caggtatcag
    49021 taacaattta tgtgcttttg ccaaattttc tttttcttca gcaaacaatt ggaacaaggt
    49081 attgaaaggc tggatctgaa tccctgtctg ctcatacaac ttatctagag aatagatttc
    49141 agaaaactgt tctactgctc cattggtacg cttgtcccga taagcaatcg gatcatttat
    49201 ccgatcccct tgttcatcaa tcagacagta atcgactgcc catgtatcga tccccgcaaa
    49261 aaggcgatca atccctgtct gctttaattt ttcaaatcca atcaaaatct ctttgataag
    49321 ataatcgatc ttccagcgat cgtgaccgtc tttgttccga aagccgtttt tgaaccggtg
    49381 tacttcttcc aagatcaaag aaccttttgc atccctcgta cttttcatta gtcgtccgct
    49441 tgatgcaccg atatcgatgg caacaaatgt tggattcatt tctccacctc ttttacttta
    49501 ttgttgatta gcactttatt aataaagcgt tatcatttat tgacaatgct atcataatac
    49561 agaatcttat attttgcttc acactatttt ggtatataat tgcattatct tgacaagttg
    49621 ggagtgttta catggattac tgggaaaaag ccaaccactc atggtcggaa gactcaaaaa
    49681 gagtgatctt gacgcctaca caaagaagca ggaatttatt tttttatatc caagaaatcg
    49741 gttacttcaa agcaccaatc ccttactata ctgaacgagc acatttacct tcttacttga
    49801 taaagtttac tttgtctggc aaaggttcgc ttacatataa tgataatcag caacagctaa
    49861 aagctggaga cttatttttt attgactgcc aagaatatca gcgttatcaa acgatcagcg
    49921 aagaaccatg ggaaatggat tggatccatt tgaatggtca gaacgtccaa tcattttatc
    49981 aggagttcac aaaagacggc cataacacct tccattcaac aggagatcca tcagaaaaca
    50041 agatccacca tttgatcaca gaattgatcc acatgcaaca aggcacgaat gcccgtaccg
    50101 atttccagtc ctctgtagct atccacgagt tgctgaatga actgattttg caaaagtatc
    50161 agctagattt cgaagaagag gatattcccg cctacgttta caaactgaag caatacttag
    50221 acgaacattt caaagaaacg attacacttg atcaattaga acaacaattc ttattgaata
    50281 aatatcagct gagcaaagat ttttccaaat ttatcggttc tccaccaatc gattatttga
    50341 tcagcaaaaa actttcttac gctaaagacc ttcttcggta tacagaattt acggttcgaa
    50401 tgatttccga agaaataggg attgagaatt ttgcttactt tagccgcctg ttcaaagcaa
    50461 aaacaggact atcgccgaga gactttcgta aattcagctg attcatataa tatgtacgtc
    50521 attttttcaa aatattttga aatatataga taaaaataaa ataaccgcag tttctgcttc
    50581 tatgtaaaca acagcagcta ctgcggttat tttttagtga tcattctcat tgttcatctt
    50641 caaaatgttc ataatgaacc aactgtttct gtgtatcacg tttcaaaaat tctttaggcg
    50701 ttatgccgaa ctctctcttg aatgctttca gaaaatgcgg ataagaagaa aaaccacttt
    50761 tactatatac agcactagag ctgcggtatt ctcgcaacaa gtgcttggca tagatcagct
    50821 ttttcttcat tacatactga tgaaacgtaa atccagtgta ctttttaaat tctcgtgtga
    50881 tatggtattt gctgacaaaa aactgctttt ccatttcctc taaagaaagc ggatgagata
    50941 aattttttga gatatattgt aacatttgtt ccacacgctc attaggaact acagcattct
    51001 gttcttgcgc actctcttct ttttgtacaa cttgattgag aaaaatcaag tattctacca
    51061 gagcttgttc atattttatg tcttcaccgt aaattttttt atcaggagga tggtcgataa
    51121 atgataattt ttgcttcaat acttcctgtt ctatttgcaa tacacgactc ctcttctctc
    51181 ccattggttg aaaacaagcc gatagattag accatggcgt ggaacgactt tctagaaaag
    51241 aaggggtgac aaagatatag acacgctcga atacatctga agattggcgg acgatacgat
    51301 gcaaatcatt ggaatggatg agcagcacac ttcctgcttt gatcgtaaat tgtcgtccgt
    51361 tcaaataaaa agtcgcttct ccctttaatg tagcatggat ctcatagaaa tcatgatgat
    51421 gatagatcgt tttattttgg ctgacgacat ctttgacatg atagatttca tactgatcag
    51481 aaaccatcgt attgacttgt tcaccgctga ttttcttttg gagcatgtgc cgcacctcct
    51541 tttgttcact tatgcacaat tttattatac cacgtttttt gtaatcaaaa agagctgctt
    51601 ttatatactc ttataaaaaa gaagagacgt ggcatcttct agccccgccc cctccattct
    51661 aaatattagt taaccaacgt cttctttaca ttattcaaga agacttcttc cataacgaat
    51721 cccatcatct tttgtttctt caagaaccac atacaggtac ggacgattct tcatgatgat
    51781 ttctttgatt tttgtgtact cgatcacacc gtctcccaaa tttaccggca cgattttttc
    51841 ctgttcgaca cgaaaatctt tcaaatgaac cgcacatatc ttttcaccaa aacgttcaaa
    51901 agcttcttct accaattgga cttgttcttg ataatcacta cttgtgatca aattggtcgg
    51961 atctaaaatg attcctagat agtccgaatc tactgcttgg atcagctgct ctacccttgc
    52021 taaagaatac accggatgat tcaaacctgg ttctatcccc accatcattt ggtatttttc
    52081 tccagctttt accaagcgac ggataacgga cacagcttca gcaaatgctt catctgtgaa
    52141 attttcttcc gtatattgga tttccggcaa aacacatcca gtttccgaag caaccatcga
    52201 cgcaccaaag gaagaagcat agcgaagata ctgctcgaac tttgtcagca actcttgtcg
    52261 tatcgttaaa tctggatgaa tcatattgat atagcaactt aaaattccta cggtcacttc
    52321 ttgcttttct aaaactcgtt tgacataatt tcccatccca gaattgatct catttgcgcc
    52381 agaaggtatt tcaggaaacg aaagacctaa ggcgagctgc agtgtatgaa tcccttgttc
    52441 ttttgttttt tctgcaagat cttctatcgt ttggacttcg gaaagatcat gtcctctaat
    52501 tgtaatgttc aacataacta ttctcctaac tcagcatatt gggtagccac atgacgattt
    52561 gcgggaagta agtaatcaaa aacaagacga ctaagataac cccataaaaa ggaagaagcg
    52621 gtttcagcat tgcctctgct tttacgcctc ctacacttgc accgacatat agtcctgtac
    52681 ctactggcgg cgtgatggag cctacacaca gattcatgat tgaaaacaac ccgtaatgta
    52741 caggatcaac ccctacactt gtgacgattg gtaaaaatat tggtgtaaaa atcaagatag
    52801 caggcgccac atccataaac atacccacca gtaataacac aagattgact aataacagaa
    52861 taataaattt attatccgtt acgcccataa tcaacgaact aattgcctga ggaataccgg
    52921 taaacgccat ggcgaaagac atcatggaag aagtagccaa taagaacatg atcgtccctg
    52981 acattgctac tgattgaaac aacatattag gtatatcttt tagttttacc gttttatagt
    53041 aaaacatagc aatcaacagg gagtaaatga cagctatcgc tgaagcttct actgctgtaa
    53101 atatccccgt caagatacca ccgataatga tgacaatcaa tagaaggcta ggaatcgcct
    53161 cttttattat ttttccagca tgagccatct gtcctttttc gacaacaggg tatttattct
    53221 ttttagcaac gatgaatgct actagcatga ttcctagcgc ccacaaagcg cctacgagat
    53281 aacctcccat aaacaaagca gagatcgaag caccaccagc aacaagtgaa tacatgataa
    53341 aagcagtact tggaggaatg accatacctg tcggagctga cgcgatattg accgctgtcg
    53401 caaacttccg gtcatatcct tcttccactt gctgagggat caacactccg ccaatcgcgg
    53461 tagaagcggc aatggccgag ctagagatgg aaccaaataa agcattccct aacacattcg
    53521 tatgagccaa agcccccggc acgcgcccga cgaacaacat cgcaaaatcc actagtttct
    53581 tagctatccc tccattattc atgataatgc ccgagagaac gaaaaacgga atagcgatca
    53641 gcgaaaaact attgatactt cccaccattt tttgagctga actaaaagca gaaacatcaa
    53701 acggtataac taacaataaa gtcatcatcg atgagaatgc aatacttata gcaatcggca
    53761 tccctaatac aagcaacaca acaaatacaa aaaataacac taatccggct tctattgcca
    53821 ttttattcct ccatttttat attttttctt gaattgctgg cttgttgctt aatgtatcga
    53881 ttagattgat caggctatag ataatgatga gtacccccga tacaggtact gacagataaa
    53941 catatcccat aggaatactt aaagaaggcg agatctgccc cattgtcaat gtgacagcat
    54001 tccatccgcc atacaccatg atcatcgcag caaataccag aaataaactt tgaacaacaa
    54061 cttctaaaat tttacttttt ttgccagtta atttttcact taaaaccgtt acggctaaat
    54121 gtgctttttg tcccacaaca tacgcggaag acaacatggc aaaccagaca agaccgaatc
    54181 gaacaagctc ttccgtgacc gtatttggat ttcctaaaac cgttctggaa aatacttgcc
    54241 ataaaacaac agcgaccatc ccaacaagca aaacagctcc caggatctct aataccttgt
    54301 ttaaaatcat tttcatcaca gctacgctcc ttgctcttgg atcttttgat aaaggtcttt
    54361 gtaggtagga tcgtttttga atatatcatg aagtggggca actgcctctc gaaacggtgt
    54421 attatctaca tcattgaatt ggactccatg ttcttcttgt gctaaagctt tttcttcttc
    54481 aattgctttt gcaaaagctt caatttcaaa aaccgttgat tctttcgctg cttcttcaat
    54541 ggctttttcc tgctcatcag ttagctgttc tttgatggca tcattcatga tgatgatatc
    54601 tggcacacgt gtatgctcat cataggaata gtatttagcc acaccaccat gtccagctgt
    54661 atacaagaca aattcattgt tttctgatcc gttgatcaaa ttggattgaa gagacgtgta
    54721 cacttcatca cttcccatag gtacgggcga accgcctaaa agctctacca ttttgatagc
    54781 ggtttcgctt tgcatcacac ggatcttctt tcccttcaaa tcttccggcg tatggatcgg
    54841 accgtctacc atataaaagc tgcgctgacc agaatcataa taggtcaatc cggtaaatcc
    54901 taaatttttg gtcgattggt agattggctc cattatttct tcattatcca tcaccttaaa
    54961 gaaatgttct tcatcattga atagataagg aactgaaaaa atcgaataat ctttagagaa
    55021 actttccaac gctgaggcac ttacttttgt gaaatctact gctcctgact gtgtcaattc
    55081 gatcagttca cgttctccac ccagttgtcc atctgggaaa tactggatct gtacggaacc
    55141 atctgttttt tcttttacta aacgcccaaa ttcattcatt gcatcaatta ctggctggct
    55201 gttgcttgca taagcaaagc gcaaggtaac tatctcattt acatctgcat ctgcgacagt
    55261 tgccacacta gaattatttc cgcagccgct tagacctagc agacctaggc taagcgaact
    55321 tgctgcaaac agcagtgatg tttttttcat atgggtttcc tttcttttaa caataagaga
    55381 cgagcaattc tgtcaaacaa agcaccgtta acgactgtcc ataaggcatt gctgtgattc
    55441 cgatattccg ataaaaatct aaactgtccc ccattcctgt cccgattgaa acgttttgaa
    55501 cttctccttt ttcatcgatt tgttcaagca atccttggat agcacgataa gcgacttctt
    55561 tatattcttg tggtaaataa cgtttatgga ccgctttcaa aataccgtaa gcaaatcctg
    55621 cagttgcaga agattctacg taagaagaag gatcatccaa caacgtatgc cataaaccgc
    55681 tttcagactg ataagttttt aatgcacgca cttgtgcttc aagtgtttcg atcaataatt
    55741 tgcgcaatgc atcttctttc gataattcta agatttcaat gatctctgga atagcaatcg
    55801 tcaaccagca gtttccacga gcccaaaatg cttcggcata gttatgattt ccttcaaacg
    55861 tccagccgtg ataccacaat cctgttcgtt tatccatcaa gtatttgata tggatcataa
    55921 actggtaacg agcttcttct aaatattctg ggcgattcaa cagtttcccg atttttgcca
    55981 aaggaagcac tgtcatcatc aatgtatcgt cccacaactg attcttattt tccggtccat
    56041 atgtggcatg ctgtaacccg tcttcatttg tccgaggcat gtcattcatg acccattctg
    56101 cccattgttc taaataaggc aaatagcgtg aatctttcgt gtcttcatac aagtaagcca
    56161 tagtcaatag cggtgccatt gtattgacgt ttttgggcgg agcgccttct tccatacgat
    56221 cctcaaacca ttcattgacc acttgataag cgcgaggatt ttttgtcatc tggtaatttt
    56281 tataaatacc atatagaccg acaccttgtg gccaattcca cactgtccag cttttgtcat
    56341 ctactttcag tccatcaaag tccaacaaaa attcaccatt atcgtctttg atcgttgtca
    56401 ggttttcaac gagcaggtca attttttctt tcactaattc tttaggtaca aaaatctctt
    56461 ctttaataat catctttacg ccaccttttt aaacgctttc attttatatc cttaatatag
    56521 cagtaatacc gtccactata aatatcgctt tttgaacaaa aactgtctaa aattgaccta
    56581 aatgttaaat aacaaaagta actttcagtt acttggaaat taaaaaaaga acttggagaa
    56641 acaacgtttt ccaagttctt tttcatctat tttcttatct tttttctacg ctttcttttg
    56701 cttatccgtt gtaagcatca atgatgatct gtttcaattc agagatcaat ggttctttcg
    56761 gattagctgt cgtacattgg tcttcgtaag ctaattcagc catacggtct acggttgtat
    56821 ctagtgtttc ttgtgtgact ccttgagctt ttagactcat gttgatgccg acagattttc
    56881 ctaattcata aacagccgtt gctaaagctt cgactaattc ctctgttgtt tcgcctttca
    56941 gacctaagaa ttttgcgata tctgcgtaat ctgtatctgc acggaaatag tcatatttag
    57001 ggaacatcgc atgtttttgt ggatcttttg cattatagcg gataatatgc ggtaaaagaa
    57061 tcgcatttgt ccgtccatga gggatcccgt attccccgcc gattttatgt gcgactgagt
    57121 gacaaatacc taggaatgcg ttagcaaacg ccataccagc cattgtagaa gcgttgtgca
    57181 ttttttcacg tgattccata tcaggtgttt ttactgattt ttctaaatag tcaaagacta
    57241 acttgattgc ttgtaggctc aatccacgtg tataatctga agccatgact gaaacatagg
    57301 attcgattgc atgtgttaac acatccatcc cggtatctgc tgtgacagat gctggaactg
    57361 acatgacgaa ttgaggatcg acgattgcga catctggcgt caatgcataa tctgctaacg
    57421 gatatttcac atgtgtttca ctgtcagtga tgaccgcaaa tggcgttact tcagaaccag
    57481 tccctgatgt tgtcgggata catacgaatt gtgttttttc tggtttgtcg atcttatatg
    57541 tccgtttacg gatatccaag aatttttgtt ttgcaccaaa gaaagatgtg tctgggtgtt
    57601 catagaacat ccacattccc ttagctgcgt ccattgcaga accgcctcca agagcgatta
    57661 ctgtatctgg cttgaagtct gccatcagtt tcgttcctgc gtatacggta tttgtcgatg
    57721 ggttcggttc aacatctgag aagacttcga tcttcacatc atttttacgt cgtgccaata
    57781 cttcacgaac tgtatcgcag taaccgaatt ggaccatacc agggtcacaa acgatcatga
    57841 cacgttcaac gttttccatt ttctctaagt acaatagaga gttcttttca aagaagattt
    57901 ttggcggtaa tttgaaccat tgcatgttat ttctccgttt cgctacagtt ttgacattga
    57961 ttaagtttac agcagagacg ttacgagaaa cagagttctt accataagaa ccgcatccca
    58021 aagtcaatga cggaatcatt tcgttataga tatcaccgat gccgccttct gcagatggtg
    58081 tattcactaa aatacgacaa gctttcattc gaagaccgaa tttgacatgc aactcttcgt
    58141 tttctgaatg gataactgct gtatgtccta atcctccaag attcaacata ccttcacaca
    58201 aatcaaacgc atggtccgtg ttgttagctt tgatcatggc aagaactgga gaaagttttt
    58261 ctcttgatag cggataatct gggcctacgc cttccaattc agcaatcagc attttcgttc
    58321 cttttggtac actgattcct gccaattttg cgatttccat cgcagaatgt ccaacgattg
    58381 atggattgac cgcatatttt ccttcgttca tcactgcatc ttctaatttt tgcaattcat
    58441 ctggttttac gatataaact tggtgtgctt ggaattctgc ttttactgct gcatagattt
    58501 ctttatccac gatcacagct tgttcagaag cacaaatcat tccattatca aatgttttcg
    58561 aaacgatcaa atcgttcact gcacgtttaa tttttgctgt tttttcaata taagctggta
    58621 cgttacctgg acctacacct aaagctggtt ttcctgtaga gtaagcagac ttgaccattc
    58681 cagctccacc tgtcgctaag acgatcgcga tacctggatg gttcattagc atagaagtcg
    58741 cttcgatcga aggatgttcg atccattgga tacaattttc aggagctccg gcagcgatag
    58801 cagcatcacg taccactcgt gctgcttctg cagaagattt ttgggcactt ggatggaaag
    58861 caaaaacgat tggatttctt gttttcaacg cgatcattga tttgaaaatc gttgtggaag
    58921 tcggattcgt tgttggagtt actccgcaaa ccacaccgac tggttcagca atttcaatca
    58981 accctttttg gacatcttca ttgatcacac caactgtttt atcatgtttg atattatgcc
    59041 aaatatattc ggacgcatac atatttttga ttgctttatc ttcgtaaaca ccgcggcctg
    59101 tttcctctac agccatcttc gctaatggca tatgttgatc caaagctgcc attgccattt
    59161 catgcacgat atggtctact ttttcttgat caaagctttc catttctttc aatgctgcat
    59221 ttgctttttc tgccaaatca tcaatcattg cttgtacgtc aattgtcttc gttttttctt
    59281 tttctattac tttagccatc attatcctcc taatgggtca tggttatttg ttaaataatt
    59341 cacatgtata tcatacaata tttatttttc tatgtaaaca cttttttgtg atttttttca
    59401 cagttatttt taaatataaa attgctttat ttacaggtgt ttgtatagaa aaatatataa
    59461 agttatttat tattatttat atgacacacc cgattattca atattgagca atatatccca
    59521 tgctcttatc tctttctttt ttgaataaaa aatgaggtag gacatctttc tgccctacct
    59581 cgtctttaaa taagctttga tacttatttt tcttccttct cttctgtctc ttccagcgtt
    59641 tctgttgttt cagtttttac ttcaactgtc tcagctggtt cttctgttgt ttcaacagta
    59701 gtcgttgcat catttgttac gacagtccct ggtttgaccg ttttgattgc tgcacgatcg
    59761 tactcaagga agatcccttc gcagtctaaa acaactgtcc gtttgtcatt gtcgatttct
    59821 gaaacaactc catgtaatcc gccgatcgtt acgactgaat cacctggttt catgctatct
    59881 aacaagtttt gacgttcttg ctgttgtttc ttttgcgaac gtgacataaa aaaccacatt
    59941 gctaatagcg cgataaacat cacaagcatt ggtaatgttc ccattttttt cacctcacta
    60001 attctgtatc tacaaaaaac actttagcag aaaaatgctg ataacactag ttttttactt
    60061 aatttttaaa agattttcct ttagaagctt tttgcgtttt ctttattgaa cccgtattct
    60121 tcaaaaaagg cttctctaaa ttcaagcaaa ttatcatcca tgattgcttg acgtacttgt
    60181 ttcattagat tcaacaagaa gtataaattg tggtaagaag tcaaacgaat cccaaatgtt
    60241 tcatcacatt tgatcaaatg gcggatatag gcacgtgtat agtttttaca tgtataacaa
    60301 tcacatttct catcaattgg acggaaatca tgggcatatt tcgcattttt cactactaga
    60361 cggccttgag aagtcataca tgtgccattt cgagcgattc gagtaggcaa gacacagtca
    60421 aacatgtcta ctccacggat aacaccatcg atcaatgaat cagcagcacc gacacccatc
    60481 aagtaacgtg gtttgttttc ggggatcaac ggtgtagtga attctaatac acgattcatt
    60541 tctgatttag gttccccgac agataacccg ccgattgagt aaccagggaa atccatcccg
    60601 actagatctt tcgcgctttg acgacgaaga tcttcaaacc ctgctccttg gatgattcca
    60661 aataatcctt gacggtctgg attagcatga gctttcaatc ctcgttctgc ccatcgagac
    60721 gtacgttcta ctgatttttt cacgtagtcg taactttcat cgaatggtgg acattcgtca
    60781 aaactcatca tgatatcaga gcccaattta ttttggatgt tgattgcttt ttctggtgat
    60841 aaaaacatac gcgaaccgtt caagtgattt ttgaaatgca cgccttcttc ttcgatattc
    60901 cgcatatcac ttaatgaaaa aacttgaaag ccgcctgaat ctgttaaaat cggttgatcc
    60961 cagttcataa atttatgcag tccgccagct tcttctacta agtcttcacc agggcgtagc
    61021 cataaatgat atgtattact taaaataacg ccagcaccca tttctttcaa ctcttctggg
    61081 gacattgttt ttactgtagc taatgttcct actggcatga acattggtgt ggggaaagtt
    61141 ccgtgtggcg tgatcaattc ccctaaacgc gcgccagtat gtttttcttt cttgattaaa
    61201 cgatagcgaa tggcaggttg cgtcattcat ttccctactt tctatataaa atatcttgac
    61261 aactcccacc atcataatcg tttcaagtgc tttttgcaag t
//
LOCUS       DACECZ010000005        51220 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.005, whole genome shotgun sequence.
ACCESSION   DACECZ010000005 DACECZ010000000
VERSION     DACECZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.005"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            330..1175
                     /locus_tag="IWO35_000350"
     CDS             330..1175
                     /locus_tag="IWO35_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0315433.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="IWO35_000351"
     CDS             1456..2676
                     /locus_tag="IWO35_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ0315434.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="IWO35_000352"
     CDS             2673..5393
                     /locus_tag="IWO35_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0315435.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="IWO35_000353"
     CDS             5377..6321
                     /locus_tag="IWO35_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0315436.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="IWO35_000354"
     CDS             complement(6390..7400)
                     /locus_tag="IWO35_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0315437.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="IWO35_000355"
     CDS             complement(7580..7924)
                     /locus_tag="IWO35_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0315438.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="IWO35_000356"
     CDS             complement(7917..8351)
                     /locus_tag="IWO35_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ0315439.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="IWO35_000357"
     CDS             8489..9226
                     /locus_tag="IWO35_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0315440.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="IWO35_000358"
     CDS             9219..10430
                     /locus_tag="IWO35_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0315441.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10637..11416
                     /locus_tag="IWO35_000359"
     CDS             10637..11416
                     /locus_tag="IWO35_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ0315442.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="IWO35_000360"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="IWO35_000360"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ0315443.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IWO35_000361"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IWO35_000361"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ0315444.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="cpaF"
                     /locus_tag="IWO35_000362"
     CDS             14132..15061
                     /gene="cpaF"
                     /locus_tag="IWO35_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ0315445.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="IWO35_000363"
     CDS             15018..16067
                     /locus_tag="IWO35_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ0315446.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="IWO35_000364"
     CDS             16064..16342
                     /locus_tag="IWO35_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ0315447.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="IWO35_000365"
     CDS             16317..16787
                     /locus_tag="IWO35_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ0315448.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="IWO35_000366"
     CDS             16804..17106
                     /locus_tag="IWO35_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315449.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="IWO35_000367"
     CDS             17123..17494
                     /locus_tag="IWO35_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ0315450.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="IWO35_000368"
     CDS             17466..17825
                     /locus_tag="IWO35_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315451.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="IWO35_000369"
     CDS             17997..19004
                     /locus_tag="IWO35_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0315452.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="IWO35_000370"
     CDS             19029..20213
                     /locus_tag="IWO35_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ0315453.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="IWO35_000371"
     CDS             complement(20650..22233)
                     /locus_tag="IWO35_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0315454.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="IWO35_000372"
     CDS             complement(22811..22954)
                     /locus_tag="IWO35_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ0315455.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23177..23656)
                     /locus_tag="IWO35_000373"
     CDS             complement(23177..23656)
                     /locus_tag="IWO35_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0315456.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23729..24013)
                     /locus_tag="IWO35_000374"
     CDS             complement(23729..24013)
                     /locus_tag="IWO35_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315457.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24091..24285)
                     /gene="ssrS"
                     /locus_tag="IWO35_000375"
     ncRNA           complement(24091..24285)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWO35_000375"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24357..25643)
                     /locus_tag="IWO35_000376"
     CDS             complement(24357..25643)
                     /locus_tag="IWO35_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ0315458.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25781..26452
                     /locus_tag="IWO35_000377"
     CDS             25781..26452
                     /locus_tag="IWO35_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ0315459.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26418..26906
                     /locus_tag="IWO35_000378"
     CDS             26418..26906
                     /locus_tag="IWO35_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ0315460.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26919..27866
                     /gene="prmA"
                     /locus_tag="IWO35_000379"
     CDS             26919..27866
                     /gene="prmA"
                     /locus_tag="IWO35_000379"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ0315461.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27867..28631
                     /locus_tag="IWO35_000380"
     CDS             27867..28631
                     /locus_tag="IWO35_000380"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ0315462.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28864..31077
                     /locus_tag="IWO35_000381"
     CDS             28864..31077
                     /locus_tag="IWO35_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ0315463.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31088..31534
                     /locus_tag="IWO35_000382"
     CDS             31088..31534
                     /locus_tag="IWO35_000382"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ0315464.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31705..32964
                     /locus_tag="IWO35_000383"
     CDS             31705..32964
                     /locus_tag="IWO35_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ0315465.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33053..34000)
                     /locus_tag="IWO35_000384"
     CDS             complement(33053..34000)
                     /locus_tag="IWO35_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0315466.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34394..35686
                     /locus_tag="IWO35_000385"
     CDS             34394..35686
                     /locus_tag="IWO35_000385"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ0315467.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35701..37470
                     /gene="aspS"
                     /locus_tag="IWO35_000386"
     CDS             35701..37470
                     /gene="aspS"
                     /locus_tag="IWO35_000386"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ0315468.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37579..38454
                     /locus_tag="IWO35_000387"
     CDS             37579..38454
                     /locus_tag="IWO35_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0315469.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38720..40603
                     /locus_tag="IWO35_000388"
     CDS             38720..40603
                     /locus_tag="IWO35_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0315470.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40678..40962)
                     /locus_tag="IWO35_000389"
     CDS             complement(40678..40962)
                     /locus_tag="IWO35_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0315471.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40952..41686)
                     /locus_tag="IWO35_000390"
     CDS             complement(40952..41686)
                     /locus_tag="IWO35_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ0315472.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41815..42438
                     /locus_tag="IWO35_000391"
     CDS             41815..42438
                     /locus_tag="IWO35_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ0315473.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42630..43751
                     /locus_tag="IWO35_000392"
     CDS             42630..43751
                     /locus_tag="IWO35_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ0315474.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43758..46886
                     /locus_tag="IWO35_000393"
     CDS             43758..46886
                     /locus_tag="IWO35_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ0315475.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46905..50099
                     /locus_tag="IWO35_000394"
     CDS             46905..50099
                     /locus_tag="IWO35_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0315476.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50245..50952)
                     /locus_tag="IWO35_000395"
     CDS             complement(50245..50952)
                     /locus_tag="IWO35_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0315477.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16760 a         8948 c        10638 g        14874 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatcccaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggttt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg agactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccatggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttta
    23041 ttttacttga actcattgac tgttttccct ttattttaaa cattataaag accgatgagt
    23101 gcttgccgcg tcttttgttt ttatcacggt tttttgtgca aaaaaacctg ccgatagttg
    23161 gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc tggcaattgg ttttctaaat
    23221 cagttcggac aacaagcaca tcgcaagctg cgtttctaat aacatattca gaaacagaac
    23281 caataaataa acgttctaca gcattcaatc ctgtggctcc taacataatc aaatctactt
    23341 gattatcttc tgggatttct ctggcaataa tttgttttgg tgagccatat tctacgacag
    23401 aggttacatt attcaagcca gctttcttcg cattgctttc ataatctgcc aatgtttgct
    23461 ttgccatttc agtagcttgt tctgcaagca ttccatcaaa ggatgaaacg gtttggaaag
    23521 aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt atttcggata gctacattga
    23581 ccgccttttt aaaagctaat tcagcttcat cagaaccatc tacggcaacc atgatttttt
    23641 tgtattgttg taacataagc attcactcct caagtcaatc gtattgtact cttttattgt
    23701 agaacacttt agggaaaaag agaattattt actgctttgg ttcagcaatt ttttttgcaa
    23761 atcggatact gaaggtggca gaaacaataa ttacgatcag taaaaagacc aaggcaataa
    23821 agcgttgatt gaaaaaagct gttaaaatac ccattacgcc taaaacagca tatgcagctc
    23881 cgtaaaattg taggaatcct tgatttttat catttttgtt gatcaataca aaaaagatat
    23941 cctgtttatt aaataaataa tagctaacga ataataatac taaagtggcg ataatcatta
    24001 aaatcctaat catgaacaaa tcctccaaaa taagcaacaa atagaaaaag caatttgcca
    24061 agagcaaatt gctttattct ttaaaagatg gtaatctccg ttaatgccgt tgtttgtcct
    24121 gtagtattga tcccgcgaat tcaagcaggt gggtcccatg gtcgtagctt cgaactacca
    24181 aatgataagg catgttacca gcagtgacac aagacgggtc ccaagatatc aataaaaatg
    24241 ttcggtcaac acacaaagag gacaattcga acatcacaga tttcccacct ctatagtagc
    24301 atatgaattt aaaaagagca atgagagagc tcgcagaaac agaaccgttt tcttgtctag
    24361 ttttttggag gatgcttttt ccactctttt atacggtaat actgttgacc cagagcctgt
    24421 tcatatttcc ctgtatcttt tggctgataa tactgagcat tcttcagttt atctggtaaa
    24481 tattgctgat tgacccaagc ctgatcaaaa tgatgaggat actgataacc cacgccacgg
    24541 ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat gatcaggaac atctccagct
    24601 ttgccctctc gaatatcagc aagtgctgca tctaatgcca tgtaggcaga attggatttt
    24661 ggcgacaagc aaagatcgac gacgacgtct gctagtggga tacgtgcctc aggcaatccc
    24721 aatttttcag cagctaatac agcgttcact gtacgagccg cagcagcagg attacctaaa
    24781 cctatatctt cataaccgat aaccatcaga cgccggcaaa tacttgccag atcacctgcc
    24841 tcaactaaac gggcgagata atgaagtgca gcatccacat cacttccacg aatcgatttt
    24901 tgaaaagccg aaataacgtc ataatgagca tctccatttt tgtcatgggt aagagctttt
    24961 ctttggacac attcttcaat gatcgacagt gtaagatgaa tccttccttc tttatctgaa
    25021 ggagtcgata gtgtagccag ttccagccca ttcaatgcac tccgcaaatc gccgttcgtt
    25081 gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga ttgcttgttg tccaaggccg
    25141 cgttctttat cccttaaagc atgttcgact gccaactgga tgtcttgttc tgttaatggt
    25201 ttgacttcaa agatctgtgt acggctgcgg atcgctggat tgatggtaat ataaggattc
    25261 tcagtagtcg ctccgatcaa gatgattcgg ccactttcta aatgaggaag cagaaaatcc
    25321 tgttttgttt tatctaatcg gtgtacttca tccaataaga ggatcactgt accactcatt
    25381 tttgcttctt ccgctacaac ttgtagatcc ttttttgaat ctgtagcggc gtttagcatg
    25441 cgaaatgcat aattagtaga gccggcgatc gcacttgcga tactcgtttt tccagtacca
    25501 ggaggtccat atagaatcat ggaagagagc atgcgagcat cgaccatgcg gcgtatgatt
    25561 tttccttccc cgactagatg ctgctgacca acgacttcat cgatcgttcg aggtctcatg
    25621 cgataagcca atggctgttg cataggattt tcctccttct tgtttttacg tcccttaatt
    25681 atatcatatt tagcaagatg cctagaaaga acagccttct gttgttttgt tttcatagct
    25741 tatccaacta atattgtgta tgataaagta ggtgagaaaa atgacggata aacaaaagat
    25801 agatgaattt ttttggaata aaacgacgcc agaagtggca cgggatttat tggggatgta
    25861 tttggagcat gacacaccag aaggaagatt agctggatat attgtagacg cggaagcata
    25921 cctaggtcca gaagacgagg ccgcccatag ctatggttta agaagaacac cgcgtgtgcg
    25981 ggcaatgtat gaaaaaccag gtacgatcta cctttacacg atgcatactc atcggatttt
    26041 gaacatcatt actcagccag aagggattcc tcaaggagta atgatacgtg caatcgaacc
    26101 cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga aaaactggtc cagatatcag
    26161 caacggtccg ggaaaattag tggaagcatt agcaattcct caagaacttt atggtcagtc
    26221 tatagcagat agttcgttac gcttagtttt tgagaaaaaa aagacaccaa aaaagatcaa
    26281 tgctttgcct agaatcggta tcccaaacaa aggtgtatgg acagaaaagc cgttgcgttt
    26341 tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa aaccaaatcg agaaagattg
    26401 gggttggagg aaagaaaatg aaaaagaagg atctattaac atatttagat gaaataattg
    26461 aaaaacaagt gacagactat gatgtagctt tagattggga tacaaaaaat catacgatcg
    26521 agatcgttgt tcgcttgttt gcagaaaata aagcacaatt tgagattgat gatgcagaag
    26581 ggatcgtttc gcaagaacca attatcgaat ttgaagacgg cgtacttctt tttaatcctc
    26641 agaaatctgt ttttgatgaa cgcaattatc tagcagtaat cccttatgaa ggaaaaaaag
    26701 ggttggcaaa atcagtagct gattctttag tggaatatct aaatgaagta ttagctcaag
    26761 ggcaaagcga tttgcttgat tttttggatg aagacgatga cgaagctgta ttcgagcttc
    26821 actgggataa tcagcaacta gcagaattag tagaagtaaa acagcaaaat gaagcagata
    26881 cttatttacc gtacccaagt tattaaggag tgacagatat gaaatggaat gaagtaaaag
    26941 tagaaactga aagtgaagca gtcgaagcag ttgctaatat tttgatggag gcaggggcga
    27001 gtggtgtcgc gattgaagat tctttagatg ttgaacattt tcagtcggat caatatggtg
    27061 agattttaga taaaaatgat tttacatcgc tgaaagaagg cgcactagtc atggcttatt
    27121 ttccggaaac gactttctta ccggaaatct tgccttttat caaagatcgt atcacacaat
    27181 tacctgagtt tggtctagct attggaaaaa ataaagtaac agttagtgaa gtagaggaaa
    27241 gcaactgggc aacagcctgg aagaaatact atcatccggt gcgtattacg cgaatgctga
    27301 cggttgcgcc aagctgggaa aactaccaga cagaagatcc tctagaaaaa attatcacac
    27361 tagatcctgg tatggcattt ggcacaggca ctcatcccac gacttccttg acattgacag
    27421 cactagaaac agtcttgcgt ggtggtgaaa cattgctaga tgtgggaact gggtcgggcg
    27481 tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt ctatgccttt gatttagacg
    27541 aagtagccgt tcgttcggcg aaagaaaata tggatatgaa tgaaatagcc aaagatgtcc
    27601 atgtatcagc taacgatctt ttgacaggaa tcgagattga aagtgatgta atcgttgcga
    27661 acattttagc agatatcatc ttattgatga tcccagatgc ttggcgttta ctgaagcaaa
    27721 caggcacgct gatcgtttca ggaatcattg aagagaagaa gcaaatggtt ctggatgcca
    27781 tgttagaaca agggtttatc gtggatcaaa ttttccagca aaaagactgg tatgcaatta
    27841 ttttgaaaaa accagaggtg gaatagatgc agcggtattt tttaaaagaa tcatatgaat
    27901 caaaagacta ttttgtttta gaaggggaaa actatcacca cattgtacgg gttatgagga
    27961 tggaacccga ggatgaagtt tttttggcat tcaatgatca aatagccatt gttgcagaaa
    28021 tcacggagat tactgaagag gctgtttatc tgaaagaaat taaaaaagaa gaaaatcaaa
    28081 aggaactgcc aagttcgatt accatagcga gtggctatcc taaaggggaa aagctggatt
    28141 ggatcgttca gaaatccact gaattaggag cgcaagcttt tcttggtttc ccggcatcag
    28201 cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg acaacaacgg cttgaaaaga
    28261 ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt gccatcagta gagttattgg
    28321 aaacgaaaca agatttattt gagcggctag atgattatga cgtggtcttg gttgcttatg
    28381 aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa aattcttagt gaaacaaaac
    28441 cagatagtcg aatcttagct gtctttggac cagaaggtgg attatcccgc gatgaagtcg
    28501 attattttgt agaacacaaa gggatacttt gtggattagg accaagaatc cttcgtacag
    28561 aaacagcacc tctttattta ttaagtgctg ctagctatca atgggaatta atgaatcctt
    28621 ctttcagcta acgttcttca ttaataaaag agtatcgaca gaaagaattg aaaaaatagt
    28681 caacaatttg tataatagaa acaattattt atacaatgga tacgaaaaat cgtttgaaaa
    28741 agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa ctgcacggat tttgggacaa
    28801 ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa aaaagaaagg gggatcgatt
    28861 cctgtgacaa aagaagaaat tttaacggga cccggtgtca taaagctcgt tagtcaatac
    28921 atgggtcccg aacatgtagc atttgttgag aaagcatgcg aatacgcgac tgctgcacat
    28981 gacggacagt ttagaaaatc aggcgaaccc tacattattc atcctatcca agtcgcaggt
    29041 atattagcag atttaaaaat ggatccccat acagtggcta caggcttctt acatgatgtt
    29101 gttgaagata cagaaatcac tttagaagat ctgagagaag aatttggcga tgacgttgct
    29161 atgttagtgg acggcgtaac caaattaggg aaaataaaat ataaatccca cgaagagcag
    29221 ctagcagaaa accaccgaaa gatgctgcta gcaatggctc aagatttacg agttatcatg
    29281 gtcaaattag ctgatagact acataacatg cgtacgttga agcacctgcg agaagataag
    29341 caaagaagga tcgctcagga aactttagaa atctatgcgc ctcttgcaca tcgtctaggg
    29401 atcagccgga tcaaatggga attagaagat acagcacttc gttatctaaa tccaaaacag
    29461 tattaccgta tcgtccactt gatgcagacg aagagagaag aacgtgaaaa atacgtaagt
    29521 ggtactgttg aagatattcg aatagcgaca gaagagctgg ggatttttgc agaaatctat
    29581 ggacggccaa aacacattta ttcaatctat cgtaaaatga aagatcagaa aaagcagttc
    29641 aacgaaattt atgacttgct agctattcga gtgatcgtag attcaatcaa ggattgttat
    29701 gctgtattag gagcaatcca cacaaaatgg aaaccaatgc ccggcagatt taaggattat
    29761 attgccatgc caaaagctaa tatgtaccaa tctttgcata ctaccgtgat cggtccggca
    29821 ggaaatccgg tagaaattca aatccgaaca caggaaatgc atgaaatcgc tgaattcggg
    29881 gttgctgcac actgggccta taaagaagga aaaaatgaaa aagtagaacc agatggtatg
    29941 acgaaacaat taagctggtt ccatgagata ctcgaacttc aagacgaaag ctatgatgct
    30001 tctgaattta tggaaggcgt aaaaggagat atctttagtg ataaagtcta cgtcttcaca
    30061 ccaaaaggag acgttactga gttaccaaaa ggatccggac cattggactt tgcatacagt
    30121 atccatacag atatcggtaa caaaaccact ggtgcaaaag taaatggcaa aatggtgcag
    30181 cttgattaca aattgaaaaa cggagatatc attgagatca tgacttctcc aaattcattt
    30241 ggcccaagtc gcgactggtt gaaattagtt gctactagca aagcaagaaa taagatcaaa
    30301 cgtttcttca aagcccaaga tcgagaagaa aatgtgatca aaggccacga atccgtggtc
    30361 aaatgtatta cagatctagg atttacgcct aaagatattt tgacgaagaa caaactgcaa
    30421 gaagcactcg atcgttttaa ttatcaaaca gaagatgatc tctatgcagc tgtagggtat
    30481 ggagaagtta gccccttgac gatggccaat cgtctgactg aaaaagaacg taaagaacaa
    30541 aaaatcgagc agcaaaagca agaagcagaa gaaatcatga atcagccgaa aaaagaacct
    30601 gacaaaatga aagtacgtca tgaaggtggc gttgtcattc aaggggtaga aaacttacta
    30661 attcgtatca gtcgctgctg taatccgatt cctggtgatg atatcgttgg ttatatcact
    30721 aaaggcagag ggatatccat tcatcgtcga gattgtccga atgttcagcc tgacaaacca
    30781 aatgtagcag aacgtttgat tgaagtcgaa tgggaagata catcgaatac acgaaaagag
    30841 tatgatgcag atttggaaat ttacggctat aatcgttcag gcttattgaa tgatgtactt
    30901 caaacagtca atgcgctaac gaaaaatctc aacagcgttg aagcacggac gaataaagat
    30961 aaaatggcga cgatccattt gacggttggt atccagaatt tatcccacct aaagagtatc
    31021 gtggataaaa tcaaagcagt acctgatgtc tacagtgtac gccggacgaa tggataggag
    31081 gacagatatg agagcagttg ttcaacgggt aagtaaagct gctgtgtcag tcgatcagca
    31141 agaaattgga aaaattggct acggcctact tgttttatta ggcattcatg aaaccgatac
    31201 gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa atgcgtattt ttgaagatga
    31261 acaaggaaaa atgaatatta gtgtagaaga tgtgggagga caaattttaa gtatctctca
    31321 attcacactg tttgcggata cgaaaaaagg aaatcgacca agctttgtgg aagccgcacg
    31381 accagaaaca gctattcctt tatacgaatc attcaatgaa ggaatcagaa gtcgcggcat
    31441 cacagtcgct accggacaat tcggagcaga tatgtcggtt tctctcgtca atgatggacc
    31501 tgtaacgatc attattgatt ctaaaaacaa ataaagtaat ctggttttca ctagtaaaaa
    31561 ctatgaaaca cgtcgcttaa gttagactga taaactctaa cttaagtggc ttttttgtct
    31621 gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt aagcttaaat tatacaagtt
    31681 attttttata ggaggttgct gaagatgaaa gaagtaccag tagtagatat caaaatcaca
    31741 gatttaaaaa aattgtatca aaaaggatac aacatgttgg aggagttacg ccatgaagcg
    31801 gacgctcccg tcgtcaaagc aaagattttc aataaagaag ctatcacgat ctacggatca
    31861 tcagcagcaa aagtatttta tgatccaagg aatttcaaac gaaaaggtgc tatgccaaaa
    31921 ttagtattga agacactttt tggtcaaggt ggagtacaga ctttggatgg agctgctcat
    31981 caccatcgga aaaatatttt tatggatcta atgacaccgg aaagaatgga agattatcat
    32041 cgtatcttag ataagaatct gacacaagca cttgaagcac agcatggtca atttgaacta
    32101 tttgatttaa gtaagatggt tttctttaca tctatttgcg agtgggcagg tatcaattta
    32161 tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat atcaaatctc tatgatcagc
    32221 ggaacgttta cttctccgat tgatcacata aaaggtgtcg aaaatcgcaa aaaatcagaa
    32281 aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc ctgtggctgg aaaagaaaat
    32341 gtggcattat atgcttttgc caatgccaca gatttagatg gtcagctttt acctctggaa
    32401 gtggcagctg ttgaattatt gaacatcatc cgtccaacgg ttgctctaac tgtgtgggcg
    32461 gcattgatgg gacatgcact tttcagtaga ccagatttgt atcagcaatt aaaaaatgac
    32521 ttttcgacgt tacaagatcc gtttattcaa gaaatgagac gctattatcc tttcttccca
    32581 atgctacctg cgatttcgct gaaagaagta gaagtagatg gttatcgtat accagaaggc
    32641 agttgggtta ttttagattt atatggaaca gaccatgatg aacgaacagt tgaagcgcca
    32701 gattccttta tgatcaaacg gtatgtaggg aaagcaaaag atatttccta taaagaagaa
    32761 tatgagatga ttgcacaagg cgggggaaat tttcgacaaa tgcatcgctg tgcaggagaa
    32821 tggatcactc ttcatagtct acgtgtgttt tccgatcagc tagtaaataa gtttgagttc
    32881 tctgtacctg aacaagattg gacgattcca ttcaatcagt ttccgactta tccgaatagc
    32941 cgagcgctac tttacaagaa ttagattagc ataaacttat cgaaaaaaag gaagatcaaa
    33001 cagtgctgat cttccttttt tcatattact ataaattgat aaggtcaatt gatcaaaatc
    33061 cacgaaatgc ttcatcaatg gtttgataat cttcattgga taaatgcaaa tctaaagctt
    33121 ggacattgtc agatacttgc tctggacgct tagctccagg tataactaca gacactcgtg
    33181 gattcttcat gtaccaagct aaaacaagtt gcgcaggagt ggcttggtat cttttagcaa
    33241 tggggcgaag tacatcgaca gcggttaaaa tttctctgaa acgttctcct tggaaatctg
    33301 gatttctttt tcgaggatct ccttcaccaa attgtttttc ctcaccaagt tcatattttc
    33361 ctgtcagcaa accggaagca agaggaaaat agggaacaaa actgattttg tttttttcaa
    33421 gataagggaa taattctttt tcagcctgcc gatggatcaa actgtattta tcttctacta
    33481 catcgacata tccatctgca ttcgcttctt tcaattgttc taacgtgaag tttgacacac
    33541 cgactgctcg gatttttcct gcttctttta attcgtgcaa tgtagcaact gattcatttt
    33601 ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc gatgtaatca gtttgcagac
    33661 gttttagtgc atcttccacc gattgtttga gaaactcagg agaattatca aatgttcgcc
    33721 ctttatttgg tacatgagca gcttttgtag cgatgacgac acgtgagcga tcatattctt
    33781 tttccttcag tacctcgcca atcagttctt cggaacggcc gtttccgtaa gcataagcgg
    33841 tatcgatcaa attgatcccg ctattcaaag ctgtacgaac tacttgtttt cctgtctcat
    33901 cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt tgctcctaat ccaagctgtt
    33961 ctgcgtagac ttgtgaatgt ccgattctta tttctgccat agctgattcc tcctttttgt
    34021 ctagtctaaa acaaaatacc aaaaagctga actaaaacgt ttttgtgagg ggaaatagtc
    34081 agatatctct tgactttgag actggttttt agtaggttat aagtaatatc gaatgtcaat
    34141 gaaagagcga gtaagttttt gaaactttac aagagaggat catcgaggct gaaaatgatc
    34201 taaagtttca ttagcctaaa gacactctgg agatggacga gtgaaaagcc gtcccgcgta
    34261 cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat taggtggtac cgtgtatgaa
    34321 tttcattcac ccttgttcat gatgaacaag ggtgattttt ttatttacat agctaagaaa
    34381 gggagagaaa actatgagtt tccaaagacc aaaaggaaca aatgatattt tgccaggcga
    34441 atcagaaaaa tggcagtttg tcgaagaaac agcacgttta ttattcagag attatcagta
    34501 taatgaaatg cgtacaccga tttttgaaca ctatgaagtg atatcccgaa gtgtagggga
    34561 tacaacggat atcgtttcaa aagagatgta tgatttttat gacaaagggg aacgccatgt
    34621 gacgcttcga ccagaaggta cagcaccaat cgttcgttct tttgttgagc ataaattgtt
    34681 cggcccagag tatgcgaaac cgttcaaaac tttttatatg ggaccaatgt ttcgctacga
    34741 acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc ggagtagaag catttggatc
    34801 agaaaaccct gcaactgatg tggagagcat ggtgatggca cttgatttct tcaaacagtt
    34861 ggggatcacg caaatccggc tagtcatcaa ttcattaggt gataaagaaa cgcgtcaagc
    34921 ctatcgtcaa gcattgatcg actatctttt accttttgaa tcagagctga gtgaagactc
    34981 taaacgccgc cttcatgaaa atccattacg cgtattggac agtaaagaca aacgagatca
    35041 aaaatttgtg gcagaagctc catctatctt agattatcta agcgagcctg ctcaaaaaca
    35101 ttttgatacc gttgtttcca tgctggatgc attggctgtt ccttatgaaa tcgacagtaa
    35161 tatggttcgc ggactagatt attacacaca tacgattttt gaaatcatga gtgacgcacc
    35221 aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt tatgacaatc tagtggaaga
    35281 actaggtggt ccttcaacac ctggcttcgg atttgcaatg ggtatcgagc gattactctt
    35341 gactatggag gcagaggaag tagttatccc tgcatttaat gagttggatg cttacgtggt
    35401 cgcactcgga gacgaaacaa atatcgaagc attgaaagtg gtccaagcga ttcgaaactt
    35461 tggtttttca gctgaccgcg acttcatgaa ccgtaaagca aaagcgcaat ttaagacagc
    35521 agataaagcg aatgccaaat tagtattggt gatcggtgaa gatgagcttg ctagtcaaac
    35581 gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt tttccattag aagagatata
    35641 tgaacatttt gatgaagtct atgatgaaat gactttattc gagtaggagg aaaaaaataa
    35701 atggcacaaa gaacgatcta ttgcggatta gtttccgaac aacaattaga acaaacagtt
    35761 acattaaaag gatgggtaca aaaaagacga gaccttgggg gagtaatctt tatcgaccta
    35821 cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg aaatcaacaa agaagcatgg
    35881 gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag tgactgggga agttagaaga
    35941 cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg aattcgaagt gattgcaagt
    36001 gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc aaattgaaga tgatcaagca
    36061 atcagtgacg aaatacgttt gaaatatcgt tatttggatc tgcgtcgtcc aaaaatgact
    36121 aaaaacatca aattaagaaa tgcgacaaca aaagcgattc gtcattattt agatgataat
    36181 gactttctcg atatcgaaac accttaccta gggaaatcta caccagaagg cgcaagagac
    36241 tacctagttc cttcacgtgt ccatgctgga catttctatg cgttgccgca atctcctcaa
    36301 ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt attatcaaat cgttcgttgt
    36361 ttccgcgatg aagatttacg tggagaccgt cagcctgaat ttacgcaagt agatattgaa
    36421 acaacgttct taacagcaga agaaatccaa acttacacag agggattgat tgcgaaggta
    36481 atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc ctcgtatgac ctatgatgaa
    36541 gcaatggctc gctacggcag cgacaaaccg gatacacgtt ttgatatgga attgatcgat
    36601 ctaagcgata cagtcaaaga agtagaattc aaagtcttcc aaatggctct tgaaaacggc
    36661 ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc gctactcacg aaaagacatg
    36721 gatcagttag gtcaatatgt aggacaattc ggagctaagg ggcttgcatg gctgaaagta
    36781 gaagaagacg gattgaaagg tccaattgct aaattcatgg gagaagcaac agaagcaatc
    36841 attaaagcaa cagatgcaaa accaggtgac ttgctgatgt ttggcgcaga taaaagtgaa
    36901 atcgtcgctg ctgcattagg tgctatccgg acacgtttag gaaaagaatt aggactaatt
    36961 gatgaatcga aattcaattt cttatgggta actgattggc cacaatttga attttcagaa
    37021 gaagaaggtc gatatgtctc tgctcaccat ccattcacga tgccaaaaga agaagatatc
    37081 ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag cttatgatat cgtcttgaac
    37141 ggttatgaac taggcggagg ttctatccgt attcatactc gtgagcttca agaaaaagta
    37201 ctagaaactc taggctttac aaaagaacaa atggaagaac aatttggttt cttgttaaca
    37261 gctttagatt acggattccc tccacatggc ggtattgcat taggattgga ccgttttgca
    37321 atgttattag ccggagaaga taatattcgt gaagtcattg cctttcctaa aaacggaaaa
    37381 gcagcagatg tgatgagtga tgcaccaagt atggtttcgc ctttacaatt gttcgaattg
    37441 aatattgatg taacgaatat agaagaatag ccgataaaaa ggtaaaaagg atttgtcgaa
    37501 tacgcttctt ttgacaacct tgatttaatt gaaaaacact ttataatgaa gccataacat
    37561 ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt gggcttatca tgattacacg
    37621 accaagcttt cggtatctat cgtttatgcg ttgatcgcat cagtcgcatt gaatttcttt
    37681 tatcagccag gacatattta ttcaagtggt attactggtc tagctcagat tttgacgact
    37741 ttgtcaaaat cactgatagg attcgaagta ccggtatcaa caactttgta tttactgaat
    37801 atacctttgt ttgtattggc gtggttaaaa atcgggaaga agtttacaat tttcactttt
    37861 atcacagtca ctttgacctc cctttgtatc catattttac cggaaacagt cctatctacc
    37921 gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg gactaggtat tgggctagcg
    37981 ttgagaaacg gcctatcatc gggcggtcta gatatcgtga gtatcacgat tcgtaaaaaa
    38041 acaggaaagt cgattggttc tatttcgatc tacttcaatg cattgatcgt ttttgttgcc
    38101 ggaattctat ttggctggca atatatgttt tacagtacgt tgtctatttt cgtcagtgga
    38161 aaagtaactg atgtcgttta tactaaacaa aagaagatgc aggtaatgat cgtaacgaaa
    38221 catccagaaa aagtagtaaa tgaaatccaa aactgtatgc gtagagggat cacgatcatc
    38281 aatgaagcag aaggtgctta taagcatgat aagcagacag tattgatgac gatcgttaca
    38341 cgatacgaac tgcctctact tagagaagca atgaaagctt ctgatccaca agcttttgta
    38401 agtattgcag aaaacgtcca gatacttggt cgtttctatg aggatggcat ttagatcgag
    38461 tttgacaaaa attcatgaaa tactgctgta gacagacgtt cgtttgtctg cagttttttt
    38521 ctaccatagt agaatcagca aagaaaaact gcttttttat ttgaaatttt cttaaagttg
    38581 aaaaaaagca tgtttatttt tttaaattta tgggatgata gaaaaaaata gtagagaaag
    38641 gacacgattc tacttgctat tttcacttaa tacccgtata ttacgaatga aaaaagttta
    38701 tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta aaaaacagcc gctacatcac
    38761 tgggtttgat gggctccgtt cattagctgt ggtgggtgtt atcttctatc accttctgcc
    38821 aactagtatg aaaggcggct atttgggcgt tccgattttt ttcgtcgtat ctggttattt
    38881 gatcacggat ctgctgcggc aggaatggga acaaaatgga aaaatcaata tttggcaatt
    38941 ttatgtcaga agaatgaaga gactctatcc ggcacttgtt tttatgcttg tgacagcttc
    39001 ggcttatatc actttgtttc agcgagggct gttaaacaat cttcgaggag ttgtggtcag
    39061 cagcttactg tatgtcaaca actggtggca aatcaataac ggtttatctt atttcgatcg
    39121 atttgctaat gagtcaccgt ttacgcatat ctggtcttta gcagtagaag gtcaaaacta
    39181 tttggtttgg ccgatcttgt ttgtattgtt gatggtattt gttcgtaaaa gaaaatggat
    39241 cttttatact gtgttaggta catccttcct ctccgccatc ttgatgatga tcttgtatac
    39301 accaggaggc gatccaaccc gtgtttatta cggtaccgac actcgtctgt tttctatatg
    39361 gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt ttaaagaaaa acatcccgaa
    39421 acaagcgaaa aatattctga atatcgctgg aggaatctcg cttttggcac tgattctttg
    39481 cttcttcttt ttagatgatc attacaattt catctattac ggaggaatgc tcctagtgag
    39541 tctgttatgc gtagtgctag ttgcagtgac agcacacccc ggagcaagtt tgaatcgatg
    39601 gctgacaaat ccagtattta cttggattgg caaacgaagc tatggcattt atctgtatca
    39661 gtttccagtt atgatttttt atgaagcaaa ggtgactgat attgccgatc atgttttgtt
    39721 gcatacttta gtggaaatca cattgattct aatcatcagt gaactttctt atcgatatgt
    39781 ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc caagtgatga aaggatggtt
    39841 cagcaaaccg atcatcagta agagcaaacc atgggttgtt ccaggcacat tacttgtagc
    39901 aattgcactc gttggtttcg ttacggcacc gaaaaacgct gtaacagccg atcaaaaaca
    39961 actgcaggca aaaatcgctg aaagtaaaaa attagcagaa gaaaccagaa acaattcaga
    40021 aaacaccact agttcggttg accaagcgat ccttgataag tacgagttgt ccaaggcaca
    40081 agggaataag gcacaaaaat tagaactaac ggcatttggt gattcagtta tgttggatgc
    40141 tgcaacggat ctacaagaac tttatccaaa agtagtggta gatggagatg tcggacgaca
    40201 attatatgcc agtgtaccgt acattgaaaa attaaaggaa caagatttgt tgaaagacac
    40261 tgttctgatc ggactaggaa cgaatggctc attctcggaa gctcagtttg atagtgtgat
    40321 ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc cgagtaccga cgaagcgctg
    40381 gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa aaatacgaca atatgacgct
    40441 gattgattgg tacagctaca gtaatgacca tagtgactgg ttttatgatg atcaagtcca
    40501 tccaaatcct gaagggatga cacattacat tcacttagtc agcgaagcct tgctaggagg
    40561 aagttcaagt acgaacagca gtgagacaaa cgtgacagaa tgatagctac aaaaaacttc
    40621 aggcaaaaac atttgttttt gcctgaagtt tttttatatt taccgtgttt ttaacattca
    40681 tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa taacgttctt tttgttttcg
    40741 ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg cttcttgaag aaaatacttc
    40801 tgcatggatc atcttaacag gtcttctttt ttgaagacga gtatatttag caccagtacc
    40861 attgttatgt tcctctaatc gtctagaaag atcggttgta tatccaccat aaaatgtctg
    40921 atctttacag aataatacgt aaaaataatg ttcatttgcc atagagcatc tcacgaatct
    40981 ctgcagtgta ttctccatcc atattgtaag tgatcaatgg tggagcgact ttgaatccgt
    41041 ctaatttgcc ttctttgatt ccttcaacta acaaaatatt tgcctctttt ccaggttttg
    41101 ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc ttccatgacc tgaatgattt
    41161 ctaagaaccg atcaggacga tggaccatag taaagcgtcc atttgttttt agtagcttac
    41221 ttgaaatatc gatcacttct tctaacgtcg tatgaatctc atgacgggcg attgccaggt
    41281 gtttattagg gttcgtttta tttgttggca gccctttgaa ataaggtgga ttacagacga
    41341 ctaagtcaca agaattatgt cgaacgacag ataagctgtc tttcagatcg atggtgtgga
    41401 cagtcatttg ttcatttaaa tggttaagcg cgatactacg tcttgccatg tctgctagac
    41461 gaggttgaag ttcgatagcg tcgatagccg cctgggttcg tttacttaaa aaaagtccaa
    41521 ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt tttaggtaca cgggaaaagt
    41581 gagcaagcag cacagcatct aaagaaaaag aaaacacttt ggaactttgg ataatcttga
    41641 tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg aagcatggat tccctccaaa
    41701 atgaaaaaaa tagtcgtctg tattatacta tttttattaa aaagaaccaa ggtggcttgt
    41761 caaacctcta ttttttatgc atactatgac cataagaagg gaaaggaaga gactatgttt
    41821 tatcgattta tgcgcggggt agttcgagta ctattagcct tagttaatgg aaatgcccgc
    41881 tatgagaata aagaagtttt accgcaagat gagaattaca tactagttgc tccccatcgt
    41941 acttggtggg acccattata tctggcggta gctgcagcac ccaaggaatt cagctttatg
    42001 gcaaaagaag aactgttcaa aaatccagtt ctgcgattta tattaactcg atccaatgct
    42061 tttccagtta aaagagaaaa tccaggacca agcgtcatta aaacacctgt aaagattttg
    42121 aaatcgacaa atttgagttt gatcatgttt cccagtggga caagacatgc tacagaactt
    42181 aaaggcggaa tggcattgat ttccagaatg tctaaagcga aaatcgtgcc agctgtatat
    42241 caaggtccgt tgacacttgg ggatctgttc aagcgtcaga gagtgacagt gcgtttcggc
    42301 gaaccaatcg acttatcaga tatcaagaaa acggataaag aaggtatggc ggagatcgaa
    42361 cgacgaacac aagcagcttt tgacaaattg gacaaagaag tgaaccctga tttcaaatat
    42421 gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc ggtcttttga atctactgtc
    42481 ctgttcagtt cagacttttt caatgaaaac gagggagaga cgtcaagtct gccctctttt
    42541 tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa tggatcaaag tgttataata
    42601 attagaagga cattttttga ggtgattgaa tgcgtttttt acatacagcc gattggcata
    42661 tcggaaaaaa actacaaggt tatgacttga ttgaagacca gcgtcatgta ctatcttcta
    42721 tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt gattgccgga gacttatatg
    42781 ataggagtgt cccagctgtt gatgccgtag aattatacaa tgaattgatg gtcgattgga
    42841 atttaaagga aaagatcccg atttttgcta tatcaggaaa tcacgatagc agtacgagac
    42901 tttcagcggg tacgccatgg ttcagttatt cgaactattt tttgcgaaca cagctagcag
    42961 aagcatttga accagtcgag tttaaaaacg tacaattttt ctttcttcct tactttgaac
    43021 caattgcagc gagattatat tttgaagatg acaatatccg aacgatcaag caagcggtag
    43081 ccaaggtgat agataaaatg caggagctat ttgattcaga aaaaaaacaa gtattgatta
    43141 gccatttttt tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc gaagtaggcg
    43201 ggttggattc gatccctggt gaactactta caccgtttga ttatgtagca ctaggacatt
    43261 tgcataatca agctgctttg agacaggata atgctcgcta tagcggttcc ccattgaaat
    43321 tctctttatc tgagatcaac caaaaaaaag gcgtatggat cgttgacctg acaacaggcc
    43381 tggagttgaa ttttagggag atcgaacccc tgcatgatat tgaagaaata acagcgagtt
    43441 tcaaagaatt gctggatcct gccttttatc agtccgtcaa tcgggagaat tacatctata
    43501 tctatctcac tgatagaacg atcatcccga atatgatgaa ccagcttcgg caaatatatc
    43561 ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag cgcaaacgaa
    43621 aagtactaga aatgaaagat cctcaaaaat tagcagaagc attttttagt gaaatgacgg
    43681 aagagcagct aactgcaaac caagaaaaat ggctcggaga aacgttgcaa gaaatcatgg
    43741 aaatggaatg atcaagaatg aaaccgaaaa aattacaact taaaaatttt ggtcctttta
    43801 tcaatgagac cgtagatttt tcacgtttga cagaagcacc gctttttttg atcagcggga
    43861 agacaggagc tggcaaaacg acgatttttg atgggatcac cttcgcactt tttggtgaaa
    43921 cttccggaag acttcgctct ggaaaagaga tgcgttcttt gtttgctaca ccagacgaag
    43981 aaacaagcgt gactttttct tttgaacatc agcagatgag ctatgaaatc gaacgaaaac
    44041 cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca gccaaagtaa
    44101 cgttgaccat ttttgatggg aaaggaaaag aaataaaaca aatcacaaaa cgtacagaag
    44161 cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa acagttttct caaatcgttt
    44221 tactacccca aggagaattc cgtaattttt tggtttcatc aagcagtgat aaagagactg
    44281 ttttacgtca tttattcggt actcaatttt tccaacagtt caatgagcga ttaaaagaaa
    44341 aagcaaaaca acagcaacaa aaattggatc atttagagca agagctgcat cttctgcaaa
    44401 aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg gtttgaagcc gttttagccc
    44461 aatggacaaa ccatcaggca atactcgaaa aacaaatcac ggaagaaaaa gaagctctaa
    44521 cggaacttca ggaaaaacaa aagcaactag aaaattccta ctatcgtttt gaaagcttga
    44581 aaaaaagtta tgaagaaaaa acgatattaa taaacaagca aaaggaatta gctgctcaaa
    44641 aagacgaaat agaggaaaag aaacgatgga ttgggtatta tgaatttacg caacgattag
    44701 tcgaaccaat ccagcgccta caagaaataa aagttgaaaa aatagaactg tcacaaaaaa
    44761 gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa ttatgaagag tggcaaaaaa
    44821 atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga agctgctcaa aaactgcaaa
    44881 acaaacagat gatggtgcca attgtcgaat ccttacgtag taaaaaacaa gacgcagaac
    44941 aattaacaat ttcattggat aaagaagaat tgaatgccga gaagctgtat caaaatctcg
    45001 ctatctatca ggaaagaact cgaattcttc aagaaaattt actggatcag gaaaaactgc
    45061 aagaagatcg tcttgctttt ggacagttaa aatatctaga tgaaaagcag caagaaatag
    45121 aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat aagagaagaa ctcaaaaaag
    45181 caactgaaga aataacaaaa aatagtcagc tattgaaaga acagacagaa caagcagaac
    45241 agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct tcagcttttt ttgcgtccag
    45301 gggagccatg tcttgtttgt ggatcaacgg aacaccccat acaacaatta aaacacgaac
    45361 agccaaataa ggaagatatc ttaaaagtag aacaacaact ggcagaagct gagtctgctg
    45421 tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca tgaaaataat cttcaacaac
    45481 aattatcaga tttagaacaa aaaatcatgg caacgaatat acaattggct gaacaagaag
    45541 aacacttgca acaggctttg aaaaaggcat ttcctgagat aacagaacaa aaaacaaaaa
    45601 caactcttgt agtaatcgaa caatctttag atagacgaaa aagcaagatg gccaatgcaa
    45661 gaatagagct ggaagaaata agacaacaag cagaaaagct cgaacaacaa ttagctgtct
    45721 acaaacagca gcagacagaa aaaaataatc agctccagca actgcaagga gaaatcaaaa
    45781 gtttagaaca acaattgcaa gatacggata gtaactgttt agaagaggag atacgacgac
    45841 ttgaaaaaca gttagaagaa gactcgaatc aactagctgt tgagcagaaa gcaggagtgc
    45901 aattgttgca agatcttcat acgcttcgga cacagcttga tcttcaacat gaacaaatag
    45961 aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat agctaaaaag ttagaaagcc
    46021 agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc acaacagcaa gcacaatata
    46081 aagaaaacca acgtacagtt acttcttatc atgaagagca attagtgaca gctgaccggc
    46141 tgcgtcaatt agaaaatatc cagctaactg agacattccc agatacagaa gagatcagcc
    46201 aacagttaga agaaataaga cgtatgatcg aaataaagca agaaaagata tacggtttac
    46261 aagaacaaaa aattagtaat caaaaaatct acgatgaatg tctttcgatt tatcacacaa
    46321 gccagacgaa attagatgag cttacgcaat tacagcagct ttcgcaaaca ttcaacggag
    46381 aaaatcctaa gaagactagt ttggaacggt atgtgcttca ggtttatctt caagaagtgt
    46441 tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa tcgttatcaa tttgagctag
    46501 cggataccat tggcagttat cgagggaaaa ccggattaga aatcaatatc tatgatgatc
    46561 aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg tgagagtttt attgcagcac
    46621 tttctttagc attagctctt gccgatgtga tccagacaca agctggtggt gtagcaatcg
    46681 atgcattgtt tattgatgaa ggatttgggt cattagatga agaagctttg gaaatggcaa
    46741 tggaagcttt agaaacgatc gaaagtgaag gaagaatgat cggcatcatt agtcatgtaa
    46801 gagaattgaa agaacgagta ttacaacaaa taagaattga tacagaaggt agcggacaaa
    46861 gcaaaatacg ctatcaactt ggataaaaag ggagattggt aactatgaaa tggagtattc
    46921 cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga aggccgagtg ttatcggttg
    46981 tccctgacca agaagaaaac gtgtggcatg cagaagtctt gggcagtgaa ttgtatatgg
    47041 ttgatttgga tggtacagca aaagagatag attactgcca atgtccctat tgggaggaac
    47101 ataaatattg taagcataca gttgctgtgg aactttattt acggtcgaaa aatctatctc
    47161 gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa agcaacaagt gagggagaaa
    47221 tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg gtgcaacctt
    47281 tgattattga ataccaagta gaaacgattg agactaatca gtatcaccca gagctttcta
    47341 ttttggcact ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca tatatcgtaa
    47401 aaaacatcta tgatttttta caagcttaca cagaaaaaga aacttacact gttaataaac
    47461 agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa atactcacgc
    47521 atcttgcagg aagtgctcag acgcatcaat tattaggtac gaacggctta caagtcaaag
    47581 gaaaactaga taaaagatat ctgcttctcc cagtagaaca agcccgcttc ctgttagagc
    47641 agatgaatca gactacacgt ttgcatctgc agataaatga acaaaacgat caaggaatac
    47701 aattttccga tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga gaagattatt
    47761 tgctgcaagc attgaatgat tttgattttt tctttatgca ttatcagtgg ggagttatcc
    47821 aagggacatt ttattctctg acgaaatatc aacaaacggt ttatcaaact tggaaacaat
    47881 tgattcgccg attggagaaa ccagaagtag tgttcaagaa aaaagaatta ccgtctttgt
    47941 ttaaagagat tatccctctg ctgtcagaga tcggcgacgt agctattgcc cctgcagtag
    48001 aagaggaagt agcagatatc cccgtcgaat ttattttctt tttccgaaaa gcaaaaggaa
    48061 aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat ctattcgact gacccgaagc
    48121 atgaggtctc gacagaacag gcttttcagg tgttacgtga cacagtagaa gagaaacgag
    48181 tacttgatct attcagaatg tatcgctacc agaaaaatga aactggttat gaacgtccat
    48241 tgccaagcgg ggaagaacta tatgcctttt tccgcacaga actatctgtc ttccgtcagt
    48301 ttggtgaagt cagacttgga agaaaattgc gagagttgta tttagatgcg cataaacatc
    48361 aaccgaaaat cgaagtaacc gagaccggtt catggctcga tattcgattc gatgtgacag
    48421 ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat gatgcttttt
    48481 atacattaga aaatggtcag gttctttctt ttgattcaga agaatttcaa caaacaagcc
    48541 aagtgttgca acaattacgg gaatcgatac gtacagaaga aggaacgatc catgtaccta
    48601 aaaatcaagg attgatcatt caaaatcaat tggaaaaaag caatgctact tttagtgaat
    48661 cgttccaaac gatggtacaa gatctcattc atccagaaag ataccaggca cagcttccca
    48721 aaggattgaa tgcaacgatg agagattatc aaaagcaagg ttttcgatgg ttgaagatgt
    48781 taggtcatta tcagtttggc ggtattttag ccgatgaaat gggacttggg aaaacactac
    48841 agacaattgc ctttttactt tcggaaaaag aagaacggaa aagcttctct gctttgatcg
    48901 tagcgcctgc gagcttaatt tacaactggc aggcagaggt gagaaaattc gccccttctt
    48961 taagtatcca agtaatcaat gggaataaaa aagaacgtga agaattactg gctaaagaca
    49021 cagatatccg agtgacttct tatgccagct taagacagga tctagctaat tatcagtcgc
    49081 aaaaaattga ttatttgatt cttgatgagg cacaaatggt caaaaatagc agtacgaaaa
    49141 ctgcacaagc attgagagaa ttagctgtac ctcagcggtt tgcactgagt gggacaccaa
    49201 tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac gattatgcca ggatttttcc
    49261 cgactaaaac aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg attcgaccgt
    49321 ttattttacg gcgagataaa caaactgtgt tgaaagactt gccggaaaaa acagagatga
    49381 atctatattc tgcacttaca gaagaacaaa aaactgttta tttagcttac ttgcgtcaaa
    49441 tgcgtgaaga aatcacatca atggattctg aagcattcaa gaaaaaccgg atcgggatac
    49501 ttgctggtct gacaagactg cgacaaattt gctgcgatcc tcgtctgttt attgaagatt
    49561 accaaggcgg ttccggcaag ctcgaacaag taaaagatct actggtagct gccaaagaga
    49621 acaaacgaag agttttgctg ttttctcaat ttacagggat gctgactatc ttgcaagaag
    49681 agttagccga gctagggatc tcgacatttt atttaagagg cagtacgaaa ccacaagatc
    49741 ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg atttccttaa
    49801 aggctggggg aactggattg aatctcacgg gagcagatac ggtcatcttg tatgaccttt
    49861 ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc acatcggatc ggacaaaaaa
    49921 atgtggtaga agtgtggcgt atgatctctg aaggaacgat tgaggaacgc atggattcac
    49981 tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa gaacagttga
    50041 cgaaactgac tgaagaagac atccgcatga ttttaagtgt aggagaaatc gaggagtaag
    50101 agactcgaaa aagcgttcag taatgggtaa gaaagccttt ctaaagaagc gattgtttat
    50161 ttgatttaaa aaatagcaag gaggctggga ttccatttta tcccagcttc cttgctatca
    50221 tttttttatt tctattttta aaggttatac aaagattgtc ttcattgtct ctaacagctg
    50281 atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt tcaattttgc gttctcgatg
    50341 gttgaaccaa agcgcttgcc agttagcact ttttgcacct agaacatcat tgtcgtaatt
    50401 atcgccaacg tataaggtct cttctggaag catatgaaat tctttctcag ctagctgaaa
    50461 aatctctttt tctggttttt ggaaacctgt tgcttgagaa atgagcatat tgcgggaagg
    50521 aatccaattg ttcaattgaa gttgcttcag tttttttgtc tgatggtcag tcggcccatt
    50581 tgtgataatt cctaatggga cattcttttc cttcaagaaa tcgagggttt ttttgacctc
    50641 ctcatgcaag cagatattat caagttcttc ctcgtaaatt ttttgaaata agaggccatc
    50701 ttcttccgta atatgagggt agtctaaatc ttttagtgat tggctgatcc gatgtgctcg
    50761 catatattct agtgtccaat ctcctgccat tacttttggg aagttttcat cactatgatg
    50821 gcggaaccgg atatatagtg gatgcatatc tgcatctgaa acaagcggaa cgactctttt
    50881 gaccgcgttt cgaaatggct gctgctgatc atacatcgta tcatcaacat caaacacaac
    50941 tgatttaatc aaaattttac acgcctttcg gttttcattg attttttcac aaatccttga
    51001 ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat aaaggttttt cttactatat
    51061 ttctatttcc ttgacagaat aaggttttta tatttcagtt tttttcacta aaaatcgtga
    51121 aacacgtcaa taagtttgat gaacaaatta atcattatgg tagaagtcga ttgatttatt
    51181 ttaatgcaat ctctgtggaa ttttcgaaag atttattttt
//
LOCUS       DACECZ010000006        48745 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.006, whole genome shotgun sequence.
ACCESSION   DACECZ010000006 DACECZ010000000
VERSION     DACECZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.006"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            23..904
                     /locus_tag="IWO35_000396"
     CDS             23..904
                     /locus_tag="IWO35_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0315478.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            917..2527
                     /locus_tag="IWO35_000397"
     CDS             917..2527
                     /locus_tag="IWO35_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ0315479.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2604..3263)
                     /locus_tag="IWO35_000398"
     CDS             complement(2604..3263)
                     /locus_tag="IWO35_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0315480.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3546..4211)
                     /locus_tag="IWO35_000399"
     CDS             complement(3546..4211)
                     /locus_tag="IWO35_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315481.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4358..4963)
                     /gene="dhaL"
                     /locus_tag="IWO35_000400"
     CDS             complement(4358..4963)
                     /gene="dhaL"
                     /locus_tag="IWO35_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ0315482.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(4991..5974)
                     /gene="dhaK"
                     /locus_tag="IWO35_000401"
     CDS             complement(4991..5974)
                     /gene="dhaK"
                     /locus_tag="IWO35_000401"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ0315483.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(5976..6371)
                     /gene="dhaM"
                     /locus_tag="IWO35_000402"
     CDS             complement(5976..6371)
                     /gene="dhaM"
                     /locus_tag="IWO35_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ0315484.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6525..6845
                     /locus_tag="IWO35_000403"
     CDS             6525..6845
                     /locus_tag="IWO35_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ0315485.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6957..7826)
                     /locus_tag="IWO35_000404"
     CDS             complement(6957..7826)
                     /locus_tag="IWO35_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0315486.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(7922..9226)
                     /locus_tag="IWO35_000405"
     CDS             complement(7922..9226)
                     /locus_tag="IWO35_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0315487.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9223..9732)
                     /locus_tag="IWO35_000406"
     CDS             complement(9223..9732)
                     /locus_tag="IWO35_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315488.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9907..12042)
                     /locus_tag="IWO35_000407"
     CDS             complement(9907..12042)
                     /locus_tag="IWO35_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0315489.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12125..13780)
                     /locus_tag="IWO35_000408"
     CDS             complement(12125..13780)
                     /locus_tag="IWO35_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0315490.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13782..14096)
                     /locus_tag="IWO35_000409"
     CDS             complement(13782..14096)
                     /locus_tag="IWO35_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0315491.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14266..14526
                     /locus_tag="IWO35_000410"
     CDS             14266..14526
                     /locus_tag="IWO35_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ0315492.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14540..14854
                     /locus_tag="IWO35_000411"
     CDS             14540..14854
                     /locus_tag="IWO35_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ0315493.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15584..16138)
                     /locus_tag="IWO35_000412"
     CDS             complement(15584..16138)
                     /locus_tag="IWO35_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ0315494.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16264..18192)
                     /locus_tag="IWO35_000413"
     CDS             complement(16264..18192)
                     /locus_tag="IWO35_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0315495.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(18498..19214)
                     /locus_tag="IWO35_000414"
     CDS             complement(18498..19214)
                     /locus_tag="IWO35_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0315496.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19215..21038)
                     /gene="glpO"
                     /locus_tag="IWO35_000415"
     CDS             complement(19215..21038)
                     /gene="glpO"
                     /locus_tag="IWO35_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ0315497.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21052..22548)
                     /gene="glpK"
                     /locus_tag="IWO35_000416"
     CDS             complement(21052..22548)
                     /gene="glpK"
                     /locus_tag="IWO35_000416"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ0315498.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22684..24144)
                     /locus_tag="IWO35_000417"
     CDS             complement(22684..24144)
                     /locus_tag="IWO35_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0315499.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24134..25429)
                     /locus_tag="IWO35_000418"
     CDS             complement(24134..25429)
                     /locus_tag="IWO35_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0315500.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25761..26438)
                     /locus_tag="IWO35_000419"
     CDS             complement(25761..26438)
                     /locus_tag="IWO35_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ0315501.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26468..27559)
                     /locus_tag="IWO35_000420"
     CDS             complement(26468..27559)
                     /locus_tag="IWO35_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ0315502.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27660..28094)
                     /locus_tag="IWO35_000421"
     CDS             complement(27660..28094)
                     /locus_tag="IWO35_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0315503.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28678..30135)
                     /gene="ascB"
                     /locus_tag="IWO35_000422"
     CDS             complement(28678..30135)
                     /gene="ascB"
                     /locus_tag="IWO35_000422"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0315504.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30132..32027)
                     /locus_tag="IWO35_000423"
     CDS             complement(30132..32027)
                     /locus_tag="IWO35_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0315505.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32085..33860)
                     /locus_tag="IWO35_000424"
     CDS             complement(32085..33860)
                     /locus_tag="IWO35_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0315506.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33910..35406)
                     /locus_tag="IWO35_000425"
     CDS             complement(33910..35406)
                     /locus_tag="IWO35_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0315507.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35600..37222)
                     /locus_tag="IWO35_000426"
     CDS             complement(35600..37222)
                     /locus_tag="IWO35_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0315508.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37671..38984)
                     /locus_tag="IWO35_000427"
     CDS             complement(37671..38984)
                     /locus_tag="IWO35_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0315509.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39229..39453)
                     /locus_tag="IWO35_000428"
     CDS             complement(39229..39453)
                     /locus_tag="IWO35_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315510.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39473..39841)
                     /locus_tag="IWO35_000429"
     CDS             complement(39473..39841)
                     /locus_tag="IWO35_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0315511.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(40038..40748)
                     /locus_tag="IWO35_000430"
     CDS             complement(40038..40748)
                     /locus_tag="IWO35_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315512.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40766..41263)
                     /locus_tag="IWO35_000431"
     CDS             complement(40766..41263)
                     /locus_tag="IWO35_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0315513.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41300..41752)
                     /locus_tag="IWO35_000432"
     CDS             complement(41300..41752)
                     /locus_tag="IWO35_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0315514.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41887..42492)
                     /locus_tag="IWO35_000433"
     CDS             complement(41887..42492)
                     /locus_tag="IWO35_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0315515.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42942..43562)
                     /locus_tag="IWO35_000434"
     CDS             complement(42942..43562)
                     /locus_tag="IWO35_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0315516.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43564..44433)
                     /locus_tag="IWO35_000435"
     CDS             complement(43564..44433)
                     /locus_tag="IWO35_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ0315517.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44456..44863)
                     /locus_tag="IWO35_000436"
     CDS             complement(44456..44863)
                     /locus_tag="IWO35_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0315518.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44882..46171)
                     /locus_tag="IWO35_000437"
     CDS             complement(44882..46171)
                     /locus_tag="IWO35_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0315519.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46198..46500)
                     /locus_tag="IWO35_000438"
     CDS             complement(46198..46500)
                     /locus_tag="IWO35_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0315520.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46525..48519)
                     /locus_tag="IWO35_000439"
                     /pseudo
     CDS             complement(46525..48519)
                     /locus_tag="IWO35_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
BASE COUNT        13660 a        10374 c         8342 g        16369 t
ORIGIN      
        1 tagaaaaagg agtggggttt ttatggcaga aatcgtcaaa gtcacacatt tgaaaaagag
       61 ctttggtcgt ttcgaagctt taaaagatgt ctctttcacc gtacattcag gtgaagtcgt
      121 tggatttatc ggaccaaatg gttctggaaa atccacgacg attcggacac ttttagggat
      181 tataaaaaaa gacggcggaa cggcaacgat ttttgggcaa gatgtatggg aagatagttt
      241 atctatccat gaacgtcttt cttatgttcc aggcgatatc tctttgtggg gaaatttgac
      301 tggaggagcg atcatcgatc tcttcatgaa gcttcatgga aaagggagtg ctcaaaaaaa
      361 agatgaactg attcaacgtt tctctcttga tcctaagaaa aaagcaaaaa actattcaaa
      421 aggaaatcgc caaaaagtgg gcttgattgc tgctttggct gttgattcgg atctctattt
      481 atttgatgag cctacttctg gtttagatcc tttgatggaa acgattttcc aagaagaagt
      541 tgaaaaattg aaagaacagg gcaaatccat cctactttct tctcatattc tcagtgaagt
      601 cgagcgacta gcggataaag tcgtgatcat acgcgaagga acactcgatg aagaaggaac
      661 actcgatgaa ttgcgtcatt tgactcgttc gaccatcact atagaaacag aagcagagaa
      721 tagtcactta gcacaattga acggtgtcca tgactttgtg caaaaaggtc acctcgctca
      781 atttgctgct gataacgaag ctttcgatgt gattttagct gaagcgtcca aattaggtgt
      841 gaaaaaattt gaatctattc caccgactct tgaggattta ttcatgagac attatgaagg
      901 atgaggagga agcgaaatga ctgataaatt ttcacgctgg tccacccttt tttctcaaaa
      961 ccttagccgt gactggaaaa agatcctgat atggctagtc ggtgtcactg ctttttctgg
     1021 tggttttgtc cctgcgtttg aagaattgac ttccggacaa ggattagctg gattatacga
     1081 aaccttgcaa aacccggcaa tgattgcaat ggtcggacca actccagctt cgaatgctgc
     1141 tgagtatact ttaggagcga tgtatgcaaa tgaaatgttg cttttttgca gtttgtttgc
     1201 tgctgtgatc tctgttttac acgtggtgag ccaaacgaga aaaaaagaag aacttggtct
     1261 gattgaactt attcgttcct ttcgagtcgg acgtcaagca aactctttag ctgtcatcgc
     1321 tgaaagtatt ctgatcaatt tgcttttagc aatatcgatc agctgtacga tgattttttt
     1381 ccaagcggat tcgatcactg cagaaggtgc ttggcttttt gggctatctg taggtagtat
     1441 ggggattttg gggagtgccg ttgcactgtt tgctgcacaa ctgatgccaa gttcagccag
     1501 ttcgaatggt ctgtctttag cgtttttagg tggactttac cttctacgag caataacaga
     1561 tgtagctgat tcctcccttt cgatgtggaa tcctttaggc tggtcttatt tgacctatcc
     1621 atttacaact aacaactgga ttcctttgct ttatttgttt ttgttcagtc taatcttgat
     1681 aatcagttca tttgttttag aggaacatcg agatttgaac accggctata tccccgaacg
     1741 tgaaggaaaa ccgtatgcta aacgttctct tttatccttg cgtggtttat tattctatct
     1801 aaataaaggg atgacaatca gttggcttat cacttttggt ttgatggggg cggcatacgg
     1861 ctctatctac gggaatatgc aacagttttt agaaagtaac gacttaatga aacagatgtt
     1921 tatccaatct ggcgtctcaa ttgaaaaaaa ctttacaagc acgatcatgg tagtcttgat
     1981 ttgtctagct gctattttac caattgctag catcaatcgt ttatttacag aagaaaccca
     2041 cggacgtatg aatcagctgt tcgcaacaaa aatttctagg aaccaacttt attggaccaa
     2101 tatgttacta gctattctat ttgctatatt ggcaatcctg attagttctg gtgcactagg
     2161 gctttctgct ttggcttcga tgaaggatgc gaaagacatg gatctattga tgtttcttaa
     2221 agccgggttc aatttcctgc ctgccgtttt agtgtttatc ggaattagtg gctttctatt
     2281 cggatggctt cctcgaatcg gtaaacttgc ttatatctac ttagcttact cctttgcact
     2341 caattacttt ggcggaattt tggatttacc agaatggttt tccaaaacag ctgtccctag
     2401 ctgggttcca cggatgccaa acgattcttt tgatctttcc gtgttcctta cgttatcatg
     2461 tatcagcctt gcactgttcg tccttggttc tattggttat aggcgaagag acttactaga
     2521 aggataataa aaaagcatgt acaatttcat gctagcctcc aaatctgggt caaaaatcca
     2581 acatttggag gtcatttttt tatttacaag tcttctaact gctctttatc aatgatccag
     2641 acttctcgat tctttctttt tacataacca ttctcctcta ataatttaaa ttttctagat
     2701 aaagtttcgg gggtcgtacc gataaatgca gcaagttctt tcattttcat tggcaggaaa
     2761 aagtgatcct tccctgtcgc tttagctaag tcaagcagat agacagcgac tcgttcttct
     2821 actttttcca tcatcaaaaa agttgtctgc tgctgtactt ttgttaattt ctcaacgttg
     2881 attgctaaaa gtttcagact aagggcggga taatgcagta agagttccga gaaatctttt
     2941 tgtctcaata tgcaaatttc cgttttttct agtgcttcgc caaataaaga ctcatttgga
     3001 acgcctagca actggctctc tccctcatat ccgcccggct ctattactcg taaaagctgt
     3061 tcctttccgc tagcagataa ttgatacact ttcatactgc cggaggccac gatcactaat
     3121 tgaggttctc cattcggcgc aagaatctgt tctccttttt ccacatagtg atgtttgaca
     3181 agtgcgtgta ctttttcttg atcttgctgt actaagtgat tgaacaaagg aacaaatgaa
     3241 acacataaat gatcatgact cataatctct cctccttcta aacagctatt tctaataaag
     3301 aaacataact tcccttattc caactgataa ttttctttta tcatagcata aacgtcaagc
     3361 gaaccagata ccagcaaata gagtggattt ctttttgtcc cttgctttat tttccttctt
     3421 ttgagacctt aagtaattga ttcctatcaa ttttttaaat atttactttt tgaataatca
     3481 aaaacaaacc ttcactcgca agccgagagt aaggtttgtt tttcgcattg cttatttcgt
     3541 gttgtctatt gaaatttttc cgcacctagc ttttgatata gtggtgctag ttcttgtaag
     3601 gtttcagcgg ttatttgctc aacgtgttcg atttcttgcg gatagaagaa tagtttaccc
     3661 atttgaaagg ttcggtctct catcgctttt ttcaactctt cttgtttctt tgtatcggac
     3721 aaatagtcag acaaagctaa gtggtcgtca agttcatgtt ctggttgcgg ccatatgtaa
     3781 taatccttga tatcgactgt tttgctccac tctttcagta tcgcaagcaa ctggttatac
     3841 gctgcaatgc taccatatcg ttcttcgctt ttataatgtt ggtacatcaa atagatttgg
     3901 tattgttttt gattcaacaa aactgctaga caggcattct cgtcttgacg agattcgctt
     3961 cggtacgcgc tccaaaaatg actgcgtaaa ttccagccat tcgtccagct ttcgatttta
     4021 ggtttgctca tgccgtacat atcaggcaaa agtgcagctg tctggagctg aatctctttc
     4081 catttttgcc agtgttcttt atattctttc ttgatttgtt caatcttatc tgttggatgg
     4141 ctctctttga gctgtttgaa tgcgtagatt ggtcgatcaa acaactgata atctgatttt
     4201 ccaagttcca ttgtttcgcc tctttccgtt atcttttgta ttctacctga attttgcgat
     4261 atggacaaat agaggttgtg aaaaagctgt tctgcatcaa gtcataagaa cagccaaaca
     4321 aaaaactgcg atgagcatca ccgcagtttt attgaactta aggttgattg atcacatcta
     4381 aaagcgttga aaataaaatg gcactagaag cagctcctgg gtcgatatgt ccgattgcac
     4441 gctctcctag ataggaagct cgtccttttt tagctaccaa gactttggtg tgttcttttg
     4501 cctggtcaat gatttctttt gttaaattcc ctgccttcaa tgcctctgtg actggcagcc
     4561 aaacatctaa catcgtttta tcgtcagctt ctgcctttcc tcggctttga atacccgctg
     4621 ttccctgctc gatcacttcg cctaattttt cttgagaatc aatagaatcg acctcttttg
     4681 tcgctttcat catgttcatg aatgcagatc cgtataacgg accagaagcc ccaccgactt
     4741 tcgatatcat cgccatagag aatactttga atgtatcgct gattgtttca ggatgatcgg
     4801 cttgaaaagc ttcttcataa gctgaaaccc ctcttcccat gttatttcca tggtctccat
     4861 cgccaatcgg ggtatctaga tcactgagat attgtttgtt ctgattgatt gcctctgcaa
     4921 attgcgtgag ccattcttgg atttcttgaa cggttagttt catattatca cccttttatc
     4981 ttgagatttt ttaccatgaa atcgtctgta cattgctttc taaagaatct ttccattgtg
     5041 tctccgtcaa gtcgatcagt gttagagaaa gaccttgcat atctagtgag gtcataaaat
     5101 taccgacttt tcggaaaact acattgacgc ctctttcttc caacagagct aaaacatcat
     5161 tcatgaacac gaactgttcc atcaaaggcg tcccgcccat tccgttgact agtacacctg
     5221 cttcttttgg taaattcgag taatcatcta aaatcttagt aacaagttcc tttgccagta
     5281 ctttagaagg ctgcatcttt tctcgacgat agcctggctc accgtggata ccaacaccat
     5341 actcgatctc atcttctcct agttcgaatc ctggtttccc tacttctggt acagttgctg
     5401 ctcgaagcgc tagaccgatc gtcttagttg cctgtacgac cttttctccc agttctttca
     5461 actcagctag ggaagcaccg tttcttgctg catcgcccac gattttgtgt acaaggacag
     5521 tacctgcaac tccgcgtttt ccagcagtat acgtactatt ttcaacggcg atatcatcat
     5581 ctacgatcac actttctact tcaatatcgt ccatagcagc taattccttt gccatatcga
     5641 agttcaatgc atcccctgta taattcttaa cgatcagcaa aactcctttt ccactatcgg
     5701 cttccttaat agccgtctga atctgatctg gagttggaga agtaaacaca tcaccaagca
     5761 ccgctgcact taacatccct tcccctacaa acccggcatg ggatggttca tgtccgcttc
     5821 ctccaccaga aaccaaacca acttgttccg ctttctgatt cttggctaca acacgggaag
     5881 attctacacg atgaacaagt tctggatagc tttttactaa tccttccagc atttcttcga
     5941 caatattgct tggatcattc atgatttttt tcaatttatt cccatccttt ctttccggca
     6001 ttttgtgctt ctgttaagat ttgctgagta tcatctgtta taccggcagt gatggctgct
     6061 gcaaaagctc cttccactaa aggcgcatcc accagatgat aacgttgctg ctgctcttct
     6121 tccaacatgt cttttgccag ttccgagttc aataccgcgc taccgatatc cgcaaaaaca
     6181 aaataactag ctgcatcgct tgattcattg actgcttcta cgattttcat cgggtctgat
     6241 ccgatctcgc catttattcc gccgagagaa aaaatttgca cctcttcgga tttttgcatc
     6301 tgctcaatca tttctttaat accatctgtt actttttgac tatgagaaat aagcaaaata
     6361 ctttttttca caagaatctt cctttctttt acatagttgc taacgattgg tttcgctttc
     6421 attaatgaac aattcaagta tataataaat ttctaatttt gggaaaatat gtgtagtgct
     6481 tcttgaagtt tgttatactt taattaaaag agaggagtga tcgaatgatt acaacaacaa
     6541 cgaacagtgt agagggaaaa gccgtagtag cttataaagg gatcgttttt ggtgaggtca
     6601 ttactgggat ccatgcattt aaagacttgg aggcggggtt gcgaaatatc tttggcggcc
     6661 gctcaaaaag ctatgaaaac gaactaatgg gtgcaagaga agaagcactt gccgaaatgg
     6721 ccgaacgtgc aaaagcatta ggagctgatg cgatcattgg cgtaaaaatg gactatgaag
     6781 tattaggcag cgacaatggc atgctgatgg tcacatgtag tggaacagct gtctccacga
     6841 gataagtagg ggacatctgt tttaataact aaagagagat gggacatgta gtggcactca
     6901 agaattagat ttttcagtct aacttcgagg tactctacct tcccatcctc tcttttttat
     6961 ttctttttgc ggatgattcc gtaagctgct gcgactaaca cacccgcccc tgctaaggaa
     7021 taactgacag aagctgtttt ttcatttgtt tttggcaaag tctgacttgt tgatttggat
     7081 ggctctgtcg tttgctgaac tgtttctgtc gtgcttccag aggtcactga tttttctcct
     7141 gccgaatgca aaatatcttt tattcgttgg aaactagctg ctaagtcagc tttttttgct
     7201 tcaaaatcat tagcaactgt ttgtgcttcg ctgatctttc cgtggattgc ttgactggcc
     7261 gctgtgatat ccgcttgagt ttgacttagc tttccagaag ctgtttgagc aacctgattt
     7321 aattgctgat tgaattcttt tgcttgttgg atcttcccgt caagatctgt ttggacagct
     7381 tccgcttttg ctttcgcttc ggcaagcttt ttctcaagtt gtgcacggtc atttcccact
     7441 ttatttttaa gatcagctaa atcttgatcg attgctgctt ggtcttcttt caatttgttt
     7501 tttgcagctt ctaatgcttc ttcgagttct tttcttttat tttccaagtc ttgcaaatga
     7561 tcgatttggt cagaagaggt agaactattt attgatgtat cttctgaaga tgaaatcgtt
     7621 tctcctgaat tatctgtcgg tacattggaa gtcatttctg tttccgaggc accgttttca
     7681 gcaccagaag agcttatggt tgttgaaggt tctgtttgag caggttcaga agtttccgca
     7741 aaagctgtct gtcccaataa gaaaatactg gaaacagcaa ttggccagat taacggcttc
     7801 attctttttt tcatcacttt tcccatcctt actacttttt aactaatttc ttattataag
     7861 caaaaatgtt tggaagaacc ataaacacaa caggctgttt gtctaaaaaa cggtgcatct
     7921 tttatttttt tgtacgttcc gggcttttcc atgtcccgct tccataacct aaaatcgttt
     7981 tgactgctgg aatgaaagtc gtcacgatcg ttatcgcgtt cactatccag aacaaaagca
     8041 tatatagtgg tgcgaaaaga aggtatttca attttcttcg tcgatcatct acaatcagcg
     8101 aagctagcaa ctggaaaaat cctgcgacca tttcaaagca gatgaacaca aaactgaatg
     8161 taaaagtaat ataaatctgt tcgtagtttc cttgatatac ataatgcccg attaatccag
     8221 caaaaagaac tacggaaata caaaagaata ttgaccagac aatactcaat gtttgatcaa
     8281 tgaagatgat cgttcttcca atatgtttaa aaggatggag catcactttt ttgaaattcg
     8341 tcagccagac ttctgttcca ccttttgccc aacgttttct gtgtcgatat agcatcttca
     8401 acgtcacagg tacttccatg aaaaagataa tctcaggagc aaaaacagat aaccagtcat
     8461 ttaactgatg atcccaagca atactgatgt cttctgtcgc tcgatcttgt ctgaatccgc
     8521 ccacatcgat caaggcttct tttcgataca tcgtatttgc tccactgtac gcataaagac
     8581 caccgaaaac agctgactgt gttcgtttaa tgatgcccac gatacttgag aattcaaccg
     8641 tttgagattt tgcaatcaat ttggtacgat tttggacatc catatttgca gtgaccgcag
     8701 caatatgtct tgcaccaggg cgaatgaaat aattcacata cctaatcagt gcatctggct
     8761 ccggtaccgt gtctgcatca ttgcttaaaa tcaatttccc ttttgcaaag gcaagtccga
     8821 tattaaacgc gtgggctttt cctttatttt tttcgatccg aaccacacga agattagcgt
     8881 attttttcat cagtctagct aatatttctg gtgtttggtc cgttgaacca tcgtctgtca
     8941 ctaatacctc atagttgtga taattcaatt tcgtcatcaa atactcgatt gtatattcga
     9001 tcacaatttc ttcattatgg gctggcacca tgatcgtgat gaacggttcg acactcaagg
     9061 gaacatctac ccattctttt tgcttatgtt tgtataaaaa cacataacac cagacaccaa
     9121 taaaccatac aaatccgcct aaaatcggat aagaaacgag aaaaaaatac ataatagaca
     9181 aacaagtatc taagaataaa ttacctgtat gggtgattga gatcatttca gttccacccc
     9241 attttcttta tacaaatctg caatgaatgt cgtatctaga tttttctctt cggagacaga
     9301 ataaaaacgg accgtttctc tctcttcttt tgttccaaaa cgttccgtat acacttcttc
     9361 aagtaattct tgtctttttt tcaatttttc atcttcatat tcaaaagatt tatttacttt
     9421 tttgtataga tttcgattgt tccacctagt caatacaaat gaggtaatca ggaaactgaa
     9481 aaaaatcaat acaaaaaagc cgattaagaa atacagcatt ttcttttctg ataaaaaagc
     9541 gtacaaggaa aaatccttgg ctctttctgg tctcgttaat gggaaaaaga cccatataaa
     9601 aggaagaaca acgatcatcc atcctaaaag agccactaac gtttgccaaa tctttaacca
     9661 gtaattacct tttgaaaaat acttatcttt caccaaaatt gatgcaactt cttctcttgt
     9721 tacttttttc atatcctcac ctcacgtttt tttgttacga cgtaggagtt taccatacat
     9781 ttcatttctt ttcacataaa gtgttcctaa atttttccta agaaaaagaa caaaacattt
     9841 ctcatcagca aatgaaaaat attttgttct ttttatcagt tgttcttttt attttttatc
     9901 tcttaattat gttgctcttt ccaatacgac aatgcttttc attcctctag atatcgccgt
     9961 gtacatttgt ctgcgtcttc tttgctcatc tccatcgttt tcaaatggat ataagattac
    10021 atgatcaaat tccagtcctt ttgctagact caaaggaatg atttggcagt tttctaatcc
    10081 atatgaattt tttttaatcg attcttgtag cgtttgggtt tcctcttctg tttgggtgat
    10141 aactgccgct ttttctcctt ttagagaaac caaaatctca gctagtattt tcaacaattc
    10201 ttcagaggat cgaaaagagc ggtattcggg ttcttttcct tgcggtcgga tcgatacgat
    10261 gtcgatcttc tcttgatcca cagcataggt tttgaaaagc tctgtgattg ctccgctaga
    10321 acggtaacta tttctaagat cataccgatg gatcggcaga tggtagtcgt cgaaacaacg
    10381 cataatatca gcaaaagtag tggaagaatt aaagattgcc tgattctcgt cacctactaa
    10441 agtaaagtga gtttttggaa ataattcgat caatagacct aattgtgcca gggtataatc
    10501 ttgtacttca tcaatcaata gatagcgaaa tgctggtaca tttacctttt caacaagtaa
    10561 atgacgaata gctaatataa tgactgcttc atcaacagta cgtgtgggtt gccaagcata
    10621 agctgagcca tatcgttttt cataaattct ttcaaacaac tgatgctcat ccacccatgc
    10681 catttcttcg atttgtctgg ttatttttcg atattttttt tgtaaaagtt tccttgcata
    10741 agctacaata ctttgttccg aatcatcaga aatcaattta ccgaataatt ccaactgcct
    10801 atcttctgtc aacgagagta cctgattctg cacagcagga ctttttgctt gtttcaaaag
    10861 atgattcttc cattcgctca tcagtgcctt tttcgttgct tgaagcctat cgatcaattt
    10921 aggatgagca ggaactttct catatatttt ttcgatcgtt tttttagaaa ggactgtttt
    10981 tccattttta gtaagtccac ggataaaatt cggatgatct aaaaacaaag gatctgattg
    11041 ttttagatta tcgataaact ttttgctacg caaacgttct gtctgctccg atacattttc
    11101 tcccgtgata cgtttgaaat aagcctcttc accttctatt tcctctgctg aaagctgtga
    11161 aaccagctgt atgatggtta gattcagtgg atttctttca cctaatgatg gtaggacatt
    11221 cgaaatatag tcgataaacc gatgattagg tgatagaatc agcaagtcat cggatgtcat
    11281 cgtatctcga tataaataga gcagatacgc aatacgctgc atgatagcag aggttttacc
    11341 acttcctgct acgccattta ccaagatatt ttgacttttc acatcccgaa tgattgtgtt
    11401 ttgttccgat tggatcgtag cagtgatagc cttcatttca ttggtctcat cttggcctaa
    11461 gacttctaat aatacgtcat cttgaatagc tacagacgta tcaaaaaact tgaccaaacg
    11521 atctctttcc aaaagaaatt gtcgtctttc tttaataacc gtatcgattt tattttgacg
    11581 aaccgtataa gaagatggac caatcatgtt attgtaaaaa agttcggcaa ttggtgaacg
    11641 ccaatcataa acaagatttt catctgcatc atctgtgaaa tttgctgtac cgatataaaa
    11701 ggattctttt ggctcattat ccagaaagtc tacaacgact tttccaaagt agggactttt
    11761 caataaacgt tcgatctttg ctagctgatt ttctgcgttt ttctgtttga tattcatctg
    11821 atcgatttct ctatttttca tttcaatcat cgaaaaagta tccaggttat ctaagtaact
    11881 gtcaaaattc aatcgaagct cgccagccat ttctttcaac gactggtttc cttctatttt
    11941 tgccgtatcc atcgattccc gcagttcgtt ttttttatca attagctttt gatacacgtt
    12001 cttcagatat gcttcttcta actgtttttg tgtgttgacc atacacattc tcctcttttt
    12061 ttaaagtggt ggaaccgatt agtttacctc ttttaagagt cgaatgcaag ctttttaacg
    12121 agtatcattt gttaggatat atcagttctt caggtcggac agtttgatat aaagaaaatg
    12181 caccatcaag gttttttacg tggtagccag actgcttcaa gattctttcc gctagatagc
    12241 tacgtaatcc gctatgacag ctgacgatat attcttgttg gctgtctaat tcatttaatc
    12301 ggtcccgaag ttcattcaaa gggatatgtt tggcttcctt gaatttgccc ttttccaatt
    12361 cttctgctgt tcgcacatct aataaaattt ttccttttgc tagttctgtc ggcaattcat
    12421 gccattgtac ggtttcacta agcccttctg caatgttcat tgctgcatat cctagcatgt
    12481 ttactggatc tttggctgag ccaaatggcg gtgcatacgt caattctaat tccggtaaat
    12541 cgaagatcgt caaatgccct ttgattgctg ttgctaaaat atcgatccgt ttatccacac
    12601 cttttgcccc aacaccttgt gcaccataaa tctcgccagt ctctggatgg aagatcaact
    12661 tcagtaaaat atctgttgct tctggataat agcttgcatg atcttttcct gatacatgaa
    12721 tgacttcaaa agacagttcg ttttctttgg ctgttctttc acttaaaccg gtggatgcgg
    12781 cagtcattcc aaacgttcgt ataattgctg ttccaatacc gccttgattt cttcgagaaa
    12841 ttccggaaat cgtgtccgca acttgtctac cttgacgatt ggctggtgaa gcaagagaaa
    12901 taagtgcatc ttttccagtg atttgctgtt ttacaacgat agcatccccc accgcataaa
    12961 tatcaggaat attcgtttga taacgttcgt ctactaggat acccccgcgt atgcctaatt
    13021 cgattccagc ggtcactgct agctggttat caggacgaac accaatggat aaaataacta
    13081 aatcagcagg gatggtacgc ccatcatcca gtcgcaatcg tttcccgtgg tcttcaaatc
    13141 cagcgacagc atgagaagta atcacttcta ctccttgatg agacaattct ttttctataa
    13201 aagctgccat ttcttcatcc agtgttggaa gaatatgtgg tgcttgttcg atcaccatga
    13261 cttctaaacc tcttcttttt aggttttctg ccatttccaa tccgatgaat cctgcaccaa
    13321 cgatcacggc acgctttggc tgtttttcta atgcatgcat cactttatcg atatctggta
    13381 catttctaat agaaaaaaca ttgtcggctt ctgttatccc tggaatcgat ggaacaactg
    13441 gttttgcacc aggagataaa accaatgcat cgtactgttc aatgtgtttt tgaccctcat
    13501 gtacgacttc taccgttttg ttttcgggaa agatggctgt cacttcatga tttggacgca
    13561 catctaaaca aaaacgagcg gataatgatt ctggcgtttg gacaagaaga ttttcccgtt
    13621 cactgatttc tcccgaaaga taataaggca agccgcaatt tgcaaaagaa acatacggtc
    13681 ctttttcaaa aacaacgatt tctgcatctt ccattaatcg gcgaagccgt gtagcagcag
    13741 acattccacc tgctacacct ccgacaataa cgattttcat cttatttccc tcctactgtt
    13801 cgatcaaacc attgattcat gccaccacgt acattcacta catcataacc agatttcttt
    13861 aattctttag ctgcttgttt actcctcatc cctgattgac aaatcacata gacggtcttt
    13921 tctttgtctc catcatatct gtttatactt tgtaatggaa catttttagc tccttttata
    13981 tgcccgcccc ggtattctgc aggtgttcta acatcaagta acgctggatt ttccaaaagc
    14041 ttttccttta attctgggac tgttatcgat tttattgatt gaaatagcaa attcattttc
    14101 tgcacctcat ttacattttt ataaatacct catggggtat attaaacagg agctttatta
    14161 atatgtcaac cagaatgact cttcattttt ttcactactt tttatatttg cataaaatac
    14221 cgataagggg tattatctaa ttataaaaat agaggagaat gtgttatgga gattgataat
    14281 aaaaatatcc tgaaccgttt gaaacgtaca gagggacaaa ttcgtggtat ccaaaaaatg
    14341 atcgaagacg aaaaagaatg tatggatgtc atcactcaat taagcgctgt tcgttctagt
    14401 attgatcgcg taatgggcat gatcgttgca gataatctga aaaattgctt tgagaaacct
    14461 gatagtaatc cagaagaaca agcggctaaa ctagaacaag cgatcaagat gattattaaa
    14521 aagtaaagga atgatttcaa tgaaagtcgt tttccatatt gatgaattag aaaaatggtc
    14581 agaaacaggg aaaaacgtaa aaaacctgat aaaagcttct ccagaaacga cgattgtcgt
    14641 atctgtaaat ggcatcgcga ttaccggtta tctagactct gccaatgcag aattcttaga
    14701 tttgcaagga gtcgtttttc acgcttgcgc caatgctatg cgtgctaatc atatttcaga
    14761 aagttcttta cctgaacaag taatcgttgt accagcaggt gtccttgatt tagttgaact
    14821 gcaatcccaa ggttatgctt atatcaagcc ttagtcagta gaggttgcga cagaagtgat
    14881 cagcttcaag aaatttcggc ttatttttcg aagaagctac ttctggaaaa ccgtgaatca
    14941 aaggttgtga aaacgctgtc ctgcatcaag taataagaac agccaaacaa aaatagcttc
    15001 tcatattttt cgtttggttg ggcttattac cgcagatgca agcttttgaa caccgtttaa
    15061 ggaagaggtt gtgaaatcag cggtttgacc taagtattag agaaaaaaac gggaaaatag
    15121 ctcctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg aacaccgttt
    15181 aaggaagaga ttgtgaaaaa gtcgcttagt ttcgagtatt aagaaaaatt ttgtgaaaat
    15241 ggatttctac atttttgcat aaatttggct taataccgag aaactggctt ttgaataccg
    15301 tttaaggaag aggttgtaaa atcagcggtt tgacctgagt attagagaaa caaacggaaa
    15361 aatagctgct catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac
    15421 cgtttaccaa gaggttgtga gcctaccatt taactccgaa tcacaagaaa taatttcaaa
    15481 aacaacttct cctgtttttg gtttggttgg cttattatcg agaagtagct actgggagtc
    15541 tatttatcaa aaaaaggaat gcgacatagg tcacacccct tttttatttt ctttgaaaca
    15601 tctttcggta ctcgacagca tacaagacaa aagattgtaa aagaccgtaa gtgatgacgg
    15661 caaaaatgac tctcacccac agcgttggta attctgggaa aagcagcaaa aggatcatgc
    15721 caacatagat gacagcagtc gctactttgc cataccactt cgctccactt aatttgatat
    15781 ttttagatag cccgattaat ccagctacaa acataaagcc ttcttttatc aagaatagac
    15841 caaacaacag ccaaagccac ggtcgatacc atgccataga caaaatcaga aaaagttgtg
    15901 tcaatttatc tgcaaacgga tcaagcacct tccctaagtc tgttacctga ttatatctcc
    15961 gagcaatgac cccgtctaga taatcagaca aacctgagac aacaatgacc gctcctgcta
    16021 tataaaatga ctgtctcagc acaaaataaa gataaatcgg cagcaaaaaa atacggaata
    16081 tcgataaaag attcggaatc gtttgtattt ctttacgcca gtctgtgctt aatttcattt
    16141 tatcgcccta cctcctcagc actcttttct ttttatttta gcttacacgg ggaataaaac
    16201 caatgatttc actgttttta tttttcttta cagccactga tattatgagc taatttgaga
    16261 taatcacttg ctgttttcat cgtttactct tctttcttcc tgtcgtttgc gcatttcttt
    16321 tactagtact ttatgagctt tttttgatcc tcgctctagg tcacgctgcc gtttacgtgt
    16381 tttataatcg ataaacagac tgtctaaatc gtaacctgtc gggtacagat cttctgctga
    16441 acgcaccaga cgaacacgct gacgaggcac gtggattatt tttttgtcta aatatacatc
    16501 aatcgtatct tcacctatat cttcataaac taacgcttct ttttggtatt cattgacaaa
    16561 tatccgatca ccttttgcaa agagctggcg ttctttttta ttctccttcc tatttgtaac
    16621 tgctgtctta ggaagaaatt cttttttctg tgtccgatag gctttttccg ccaagtaacg
    16681 ctctgcttga tggatcaatt tcatcgacat atccatttta tgagcaatcc acaaagcttg
    16741 gctttctcct gtttctccaa cccgcaactg atatttagga cgaagtgctt cccgatcgaa
    16801 agccatagca gccggcacaa aatcttcatg atctccggcg aatttcttga tttctccata
    16861 atgggtcgtg gcaatgatca atgccccttg ctcatacata gattccatga ttgcgatcgc
    16921 tagtgcagcc ccttcatttg gctctgttcc actgccaatc tcatctaaaa gtaccaacgt
    16981 attccttttt atctttttca aaatagcagc gatgttttgc atatgaccag aaaatgtact
    17041 caacgcattt tctaaatttt ggtggtcacc gacatcgacg aaaatctcat caaagacagc
    17101 aagctctgtc ccttcttttg ccggaacttg cataccgaac atcgccatca aagtcaataa
    17161 accgactgtc ttcaacacga ctgttttgcc tccggcattt gctccggtga taatcaagcc
    17221 tcgatagtct ttaccgattt caacatccaa tggtaccgca tgctcaggta aaaatggatg
    17281 tctgccttgc ttgataatga tatgttccga tttattgatt ctaggtgtgc ttccattgat
    17341 ttctcggcta aattttgccc gagcaaagat gatatctaac accgtgatcg tttcgatcaa
    17401 taaatcgatc acttcttcgt tttctgcgat agcaccagtc aatgccgcca ggacttgata
    17461 ttcttcactg atttcttcag cttttaacaa ctgatagtgt tcattcaatt tagaaatcac
    17521 agttggttca ataaaaaccg tcgttctttt atttgattca tcgataatcg ttccatctat
    17581 tttattttta tagctggctt tgataggaat cgtatagtat tcgccttttt gaacaatcat
    17641 tgcttcttgg atcatttctt tgtttttcgg atgacgcaag aatttcagta attttgactg
    17701 gatttctttt tcgattagtt gtaattgttt gcgtacttta cgcaaattac gtgacgcgtc
    17761 gtcactgact ttattatttt ttattttctg gtcaatcaac tcttctacat ttatcaaatc
    17821 aggaagatgt ttgctataag caaacaacaa gggtgtctgg tattgattct tatcaaaaaa
    17881 ctttgtgatc atccgactgc ttctaagaaa atccgcatat tcgatcaaat cggttggctg
    17941 cagaatcaac ccttttttga cttgttctgt cagtgcatcg atcctcggca accccataaa
    18001 tggaacatgc tgactgcttt ctaagatcaa gcgtgcttct cttgtttctt cttgacgatc
    18061 caagacagtt tgtaggtttg tcgagaccgt catatcgctg attcgttttt tgctatagtt
    18121 tccaattgct cttgcttgga tttccttttt gatccgatca aattgtaatt gactaatcat
    18181 ttcgttgtcc atattctctc cttttcttga agagatacct gtcccaaaat aaataagggc
    18241 ctagagataa ataaaaggat agaacacagc ttaaaaaagt catgtcctat ccttattaga
    18301 atacaaagat attgttttcc tgacaggtac cgtttcataa gcacaccaaa taaactttgt
    18361 ttattggcta ttcgacttac tgattagata ccagctacac tcacaaaaca taccccattc
    18421 ttctaggtct tatttatttt ccacttaact atagcaagac acattttggt tgtcaaactt
    18481 tagtatgttt tatagattta caccattaat gaatagacac ctaccgcgat aattgctccg
    18541 atcattggac ctgcgattgg tacccatgaa tatgcccaat ctgaagttcc tttattagct
    18601 attggcaata ccgcatgtgc taatcgagga ccaaagtccc gagcagggtt gattgcatat
    18661 cctgttggcc ctcctaatga aagaccgatg gctaagatca atccacctac agcgaatacg
    18721 ttcgttccag gtgcaaattc gttttgtcca aaagctagca acccaagaac taatacaaat
    18781 gtaccaatta attctgtgat cacatttgct ggataattcc gtatagcagg acctgttgca
    18841 aacgttccta aaatcgcgct ttcatctttc gttgcattcc aatgtggcaa ataagaaagc
    18901 caaacaagaa tggcccctgc aaaagctcct aaaacttgcg caatgatgaa aggaacgaca
    18961 agattccaac taattgcccc tgtcatcgcc atagctagag agactgcagg attcaaatga
    19021 gctgggccca tgaatccaga aatatagaca gccattgtta cagcgagtcc ccaaccaaat
    19081 gcgatgacaa tccagccaga agcctgtgct ttacttttgt ttaaattcac agccgcacac
    19141 acaccatctc caagcaaaac taggatcagt gtcccaataa actctcccat gatttgagtc
    19201 atatcactgt tcatttattt ctcccccttt agttcccgca gatctgattc tgcaatcaat
    19261 gcttctaaac gtttagtctg ttgcgccttc tcctcttctg tccattcaaa atatccagca
    19321 attgcatcga tcacaggttg cttgatctca tcaagctgat ctcgttcaaa caacagatga
    19381 tttgtacgac gaatgagata atcgcctggg gccaaaacca tttcttcttc taaaccataa
    19441 cgtaaccgag ctgactcagc gagactcaaa ccaggataag gtgccatttc ttttgctaat
    19501 tcaaagatac gtcgggcatt agtcccgtaa aaatcagcga tataagtagc atcttcttcc
    19561 tctaaaccgg ctgctactcc ttcttttgct aattctgtca ctgtttcttc taatttagtt
    19621 ggatcgaagt ttccacctga aatctgatat tttttagagt cgatttcttt cgtctctatt
    19681 ccgtattctt cttttaataa ttggcgtatc aatcgcaaag ctccttctgc catcttacgg
    19741 taatcggtga ttttcccacc agataaagtc agcaaaccat ctggttctct ttctaaagaa
    19801 ctacctctgg aaatcgtaga aggtgctttt tcatcacggc tgttttccaa atggttcaac
    19861 acgtcttcta cttcagcacg agaaactttg ttttccttat attcacttac tgtatcgaca
    19921 actttattga agctcttgtc tgaaatcgat ccattatctc caccattgta atcagaacca
    19981 gaattaccaa tcaacagtgg acgaagtcct gcccagcttg cttcgatatc attcaatgtg
    20041 atatttgctt ctggatagcg atgattgatc acatccaata gataatctac atcttcttgt
    20101 gtgactttcg gatccgtaaa atctccttgg taatccgtat ctgtcgtacc aaagtaagtc
    20161 ttgttttctc ttggaatagc aaaaaccatc cgcccatctt gctttcctgt atcgaagtat
    20221 gtgggttgcg gtacaggcag ttttttcgca tctacaacta aatggatccc tttagttgga
    20281 cgcattttag gagagactgg acgcgtaaaa ttcaagtttc gtactttatc tacccaagga
    20341 ccactcgtat tgatcactaa ttttgctttg atttcgatca cttcatctgt cagcaggtca
    20401 cgagctttga cgccaacgat ttgatcgcca tcatataaaa agccaaccgc tttcatttta
    20461 cttactagat aagccccatc ttcggcagcc tttttgatat tatcaatcac taaacgggca
    20521 tcattgttgc ggaaatctag atagacaccc gcgcctttta gcccttcttt tttcaaaaac
    20581 ggttctcttt ccaatacttc ttctggtgtc agcgtataat tctcatattt ggttcctgtc
    20641 acatttgcca gtttgtcata aaggtccatt gctactttga ccgagaacat attgaaggtt
    20701 gttgctcctt cgccctcata gattggcaaa agcattggat ctggttttgg gatgtgtggg
    20761 gcaatacttt gtacgactgc acgttcacca actgtgtcag ctactacttc cacatcaaat
    20821 gtcttcagat aacgaatacc gccatgaaca agtttggtcg agcgagagga agtcccttct
    20881 gcaaaatcct gcatttcgat caatcctgtt ttgattcctg atgctgctga ctggactgcc
    20941 acaccggcac cagtgattcc gccaccgatg atcaacagat ccaattcttc ttgctgcatt
    21001 ttttgaatgc tatcttgtct tgtcttgttt gaaaacatat ggatttcctc cttattcaaa
    21061 tgcttgggta gcttttactg cttttttcca accgttgtaa agtttttctc ggcgctcttc
    21121 ctccatttga acttcaaata gcttgccagc ttcataaaat tcgcggatct cgtctgtatc
    21181 tttccaataa ccgacagcta atcctgctaa aaatgctgcc cctagcgccg ttgtttccaa
    21241 gttttctgct cgttggatcg gtacattcaa gatatctgtt tggaattgca tcagatattc
    21301 attgtttgct gcaccgccat ctactttcaa tacaggaatc ttgattccag tgtcatcttg
    21361 catcgtatcg atgatatctc tcacttgata agcaatcgac tgtaaagtag ctttaatgat
    21421 atcttctttt gtcgtaccac gtgtcaagcc aaacatagcc cctcgtgctg cctgatccca
    21481 ataaggagca cctagcccaa cgaatgcagg aacaacataa acctcatctt cgcttgtaga
    21541 gcgtttcgct gcgtcttctg aatcgctagc tttttgcaac atttgaagtc catctctcag
    21601 ccattgaatc gaagaaccag ccacaaaaat acttccttct aaagcatagt aaacctttcc
    21661 atttatgccg taaccaattg tcgtcaataa attatttttg gataattgcg gtttttcacc
    21721 ggtattcatg acaataaatg aacctgtacc ataagtattc ttgaccatgc ctggttcgaa
    21781 tgccatttgt ccgaataatg cggcttgctg gtctcctgcc atccctgaaa ttggtgcttc
    21841 gcttccataa aaatgataac cttgcgtata accataaact tctgagttag aagtgacttt
    21901 tggtaacatc actcgaggaa tatttagtaa atctaaaatc tcctgatccc aatctagctg
    21961 atggatattg aacaacatcg tccgactggc attcgagtaa tcagtaacat gtgcttgtcc
    22021 atcggtcagt ttccatacta accaagaatc gatcgttcca aacatcaatt cgcctttttc
    22081 agctcgttct tgcgcgcctt ctacatgatc aagaatccag cgtatcttcg ttgctgagaa
    22141 ataggcatcg atcactaacc ctgttttttc gtggaaaaat tcttgatagc cttcattttt
    22201 taagttatct gcaattccag ctgactgtct ggattgccaa acaactgcat gatagattgg
    22261 acgtcctgta tctttttccc atattactgt tgtttcacgc tggttcgtta tgccaatccc
    22321 agcaatctga tttggtttga tccctgattc gatgaacgct ccggcaataa ctgactgtac
    22381 agaattccag atttcatttg catcatgttc cacccaactt tcttgcggga aatattgcgt
    22441 gaactctttt tgagaactgc cgatgtgtct tcctttcttg tcaaagataa ttgctcgtga
    22501 gctagttgta ccttggtcaa tcgccatgat atacgttgat tctgtcattg tgattccctc
    22561 cattatatgc aaacgctttc tttaactact cttatcatac tactgtttta aaaaaagaaa
    22621 ctgtcaaaat tttattcttt ttttcttttt ttgacagttt tttattatga agctattttt
    22681 taatcaattc ttttcttttt tcagcgtctc tattcttctt ttcaatcgaa taatgtcgta
    22741 aggtgacgca aattctgaca gataatattc acactgtttt ttgtttctct ggcaaaatgt
    22801 gatcaccaaa tcatattctt taccttcttc ataacttcca atctcaatac cgccgattcc
    22861 cctcagttct ctgatcagat attgttgaaa agcaatacga taaatcggca gactttctaa
    22921 atcgattcca atcactactg gttttgcaat ataaaaatcg atcatcaaaa gaacattggc
    22981 atactggatc atcaggtaac taagctcact atcttcagct cttttcctcg gcaattgctg
    23041 caccgctgtt tcctgtaatt tcgccagtaa ctgtgttaag gaatgcccga gcattttctg
    23101 ctgctgttcc aaaagccgat cacgctcata gacttcgatc tttccaacaa agaaaaacca
    23161 ttcattgttg atctgggaaa tttggtagcc tactgccttt tcttctttga ttttcaaacg
    23221 acgaggacga taatgccata acatcgtttc ccgaatgtaa gtatctaaaa cagcggttgg
    23281 taactttttg ctccgtgtca aatcatactg ataaaatatt tcttcgtcta ctatcgaaaa
    23341 agaaacaaaa aagctgtaaa acatcattgt ttcgtataca ttcagttctg ctgctgttcg
    23401 actaagatgc atcacgatga ttggatcgat tgcttgataa agccggtccg actgatagag
    23461 taatttcttt tctttcaaag ttgcgtacgt acttttttca ttcaatagtc tttttttcgt
    23521 aatcccgagc cagcaagcaa tctgtgcttc tgctgatgcc gtaaacgttg tttctaatac
    23581 acggtccaat cctagaatga acggtctttt ttctggtgta ttttgcaaaa aaagcggtcg
    23641 ctgatcttcc gggatatatt ggaatagttc ataataaaaa tatcgtatct gtagctcttc
    23701 tccgtataat tgaccgtttt tgatttgtaa tccaaattcc tctaagagct ggttcaattc
    23761 tttgatcttt cgaaaaacag aagactcact gatattaaac gctgtcgcta atcgaacgat
    23821 ggatacttgc tgatgttcta ataaatagac gagcatctta aacttcaagc tttcctgcaa
    23881 taaaaacaat agaatttttt ccaaacttac atcttcgtcc aacacaagca gcgcctgata
    23941 ctcatttcgc tgtacttcta cttttttttc catcattcgg ccaatcaatt caatatcttc
    24001 caaatattga tcaaacgagg cttttgacag ttggacatgc tctctcaaac cattgataga
    24061 ggctgttccc ccttgcagaa caaggtttcg caaaatcagg atctgtaact gctcttttct
    24121 ttctaataat gcttcaatct tcataggcag tattcgccac ctttgaccac accatatctg
    24181 gcacacggta ttctggctgg aaaaatagct catgttcttc taactgttca aacgtccatc
    24241 cttcttctac tgcaattgtc agttggtcaa gaaggtggaa catctctttt ttgctttgga
    24301 gctgtgctcc aagaatcttt ttttcagaat caaatagaaa tttgagttta actttgcttt
    24361 gatttattgc tgtaagcttg tagtctacgt atcctgtttc aattttttgg ggataaaaaa
    24421 tgctctcttc ttctgtcaaa cctacgcttc ctaagaaata gccaaaccac tgattgccga
    24481 ctggacgata ggttccctga tcctttgtct gtgagccact aattgtcttg ctgacatttc
    24541 gtgctgtacg gacagcgtta tttactagag aaacatacat tttttcattt gttggacgaa
    24601 aagttacttg gatcgcatcg ccaatcgcat aaacgtcttt ttgagacgtt tgcaagtagt
    24661 catctaccca gatcgttcca tcatcattca atgttaattg ttcttcccac atctgattgt
    24721 cggggcgaga atgattagcc agcaaaacaa gatcatagtt ctcctttctt ttttccgtct
    24781 ctacaatagc ggtttcattt tcggtcaaag acacaacttg ttcttctaaa aaaagtgaaa
    24841 gcccttgatt tttcatttcc tttactaacg gttctgtcat ttcaggatct agataacgaa
    24901 aaagaattga ctttcggctt tcgtaaagat ggatttgttt ttttcttgat gccaaccctt
    24961 cagctatttc gattcctgct tgtcctgcac caataattgc aattttcttt gcattcatga
    25021 tcttttgctg aagagccgtt acgttttgta tctttttaac tgggtgaata agcgttgatt
    25081 ctgcagaaat atttcgaaac ttttgatttg aaccagtcgc tagaatcaaa cgatcaaaat
    25141 ccaatttttt tcctcctgct aaaacgactt ttttttcttt caatcctatt actgtctttt
    25201 ctgtgtaaac atggattcga tagcggtcag tcagttcttc tttcgtgatc caagaaagtt
    25261 gtttttcact ggtaaccttt ccttttaata tcaaattgaa cccgctggga atccatccta
    25321 acttcgattg cttttcaatc aaatatattt cacttgttgg catcaattgg cgaagataaa
    25381 tagccgcatg aatccctgca aacgacccgc cgacaattac aatgttcatc tgcattcctc
    25441 tttcttataa ggtgttctac tgctaacttc tcaatcagaa aacacagtat tcataactcg
    25501 ctttcaacct tatgatacat agcgatgcag cagaatcaac cctctccatc ataccgtgtt
    25561 ctaggtctgc ttctcaataa tgtactagtg gacctaattg aagagtatca tttctgaaga
    25621 gatgtttcaa aaaatgtact cttataaaag aaaaaaactc gccttttacg tacctctgtc
    25681 caaataggaa acagaaaaga acgaaaagac gagttttagc gtgtgtaatg taatagttag
    25741 aatagacaca tcatttattt ttaattcttt aatgcttcaa caactgcttc gcctaattga
    25801 tgagcagatt gaggattttg ccctgtaaga agccgcccat caatttgaac cacttctgaa
    25861 aaagctgctc cagactgata gtttgctcct ttttctttca aagcatcttc tgctaagaat
    25921 ggtacttcat tcgttgtacc atttgcttcc tcttcttcat tactgaaccc agtcactgta
    25981 cgtccagcta aaatagattt tccagactca tctgtaactg gcaacaatcc tacaacaccg
    26041 tgacaaacag ctgagaccag cccttcattt cgatagattt gtgaagcgac tttcccaaga
    26101 tctattgctt ctggaaaatc ccacattgtt ccgtggccac cagcataata aaccgctgca
    26161 taatcatttg catccacatc atcaggacgt aaggtatttc ccaaggcccg atttctgaaa
    26221 tcttcatctt cataataggc ccaatcgatt tcatccatat tcaaaacact ttgcggttct
    26281 aaaggtacat atccgccttt aggactgaca aaatctgcat cgatcccatt ttttactaat
    26341 acatcataaa aatgagtcag ttcacttaac cacaaacctg taggacgctc gatcttttcg
    26401 tacttttcta catttgttaa tacgatcaat actttcattt tcccatcctc tttctataaa
    26461 aattgcgtta ttgataacgt gctttcaaga aattatataa tgggcttggc aaataaagct
    26521 ctttatatgc ttcttcaaaa ggtttcgtta caaattcatc ctcacggttt tccaataatt
    26581 tctctgccca gtcaggatcg acgatcattt gctgtccaat tgctgcaagt tctgcatatt
    26641 tcaatacctg ctctacatct tcgcgattgc ggatcccacc gacaccaact aaagggatct
    26701 ttccttgaat cgtgttatgg accacttcaa gaatcgattt atcgttgtat ttatcagaac
    26761 gagctattcg atcatacata tttaatgaaa catgcagata atctagtttt gactcacgta
    26821 atcgttcaag aagccagatc gtgtcttcaa aacggatacc tggtgtttca tattcttctg
    26881 gtgagaaacg ataccctacg atgaatggtt ttttagcata ttgttcaacc gctgagaaaa
    26941 ccgttgttaa taattcttca ataaaacgat aacgattttc caagctaccg ccccattgat
    27001 cctctcgtct attcgaatgc ggagagaaaa attgttggat caaatacgta tttgctccat
    27061 gcaactcgat tccatcaaat cctgattcaa tcgctctacg tgtggctttg ccaaatgctt
    27121 ggattgtttc aacgatttct tcttcactca ttgctcgtgg tgtttccgca tcaggacgtt
    27181 ttgctgcaac tgcacttgca gatacaggct gttctccaga taaagttgct cgacttgtca
    27241 ttcgtccacc gtggaaaatc tgaataatcg cttttgcgcc ttctccttgg attgctttcg
    27301 ctagctcact taatcgagga atcatttcat catgagcgat gctgagttca cctggccagc
    27361 cttttccgat atcttggaca tttgcagcac cagtaataac agcaccaaga cctttagaac
    27421 gttgtttata ataagtgatc tcatcttcgg tgatcactcc attgaaaaag ctgagttgtg
    27481 tcgtcatagg cgacagcatc aatcggttct tcaattgaac tcctgaaggt aaagtaattg
    27541 attgatcaaa ttgtgacatt gtcattcctc ccataattcg ctctgattaa cctatcgaga
    27601 atcatctatc attaaaccac tcgattagtt gtacatgcaa ctaatttgct taaaaaattt
    27661 tatttttttt gtaacttatc tgttgcatca ttcattgtct gggctaattc tttacttgct
    27721 tcatttccca aaactgcttt gtatgactga tgaagttctt cccagctacc atcgacttcc
    27781 ttagcaaatt ggcgtccttg ttctgtaaga taaactaatg acaagcgacc ttcgctaact
    27841 gttttgacgt atcctttata ttctaatttt tcaacaaaac gagcaatcgt tgatggggcg
    27901 gaatagagca tttgtgcaat atctttttgt ggtattccat ctttttcttt taatatcaat
    27961 aaaataaaac cataagttgg tgccagacct gtccttctaa acgcttcatc agctaattta
    28021 ttcaacatgc gatctagctt tttcacgcta aagaaaagac attcatctaa cgctttttca
    28081 attggctttt ccatttcatc ccctccattg gcgtacatat catagtatga tttttatttt
    28141 ttgtcaaaaa acacggaata atgtccgcct cctctgtcta gatttgaaaa aaagaagtag
    28201 ccatcttcaa agaatttcgg cttgttttcg acaaaactcc ttctggaaca ccgtgaatca
    28261 gaggttgtga aaaagtcgtt tagttttgag tatgaagaaa aattttgtga aaatggcttt
    28321 ccacattttt gcataaattt ggcttaatac cgaaaaactg gcttttgaac accgtttata
    28381 tcagaggttg tgaaaaagcc gtcctgcatc aagtaataag aacagccaaa caaaaatagt
    28441 tcctcatatt ttgtcgtttt ttgagctaat gccgatggtc agctgattgt acaccgttta
    28501 ttgagaggtc gtgagcctgc cgttgaactc cgagtcacaa ggagcaattt caaaaaacag
    28561 cttctcatgt tttatgacta tggtgacttg tgatcgagga gttggcagga gaatactgtt
    28621 tattcgtaaa gaaggggctg tgacaagact tttgtcacag tcccttattt ccttttatta
    28681 taggatttcg ccatttgatt ggatgacttg tctgtaccaa tcaaatgatt ttttctttaa
    28741 ccgtttcttt gtgccattcc cttcgtcatc ttgatcaaca tagatatatc cataacgttt
    28801 tgacatttgt gaagtagaag cgctgacgat atcaatgcag ccccacgacg tataaccaaa
    28861 taaatctact ccatctttga tagcttcctt catctgctca atatgctgtt tcaaatagtc
    28921 aatgcggtaa tcgtcttgaa cggttccatc ttcttctact ttgtctatag ctccgagtcc
    28981 gttttcagct atgaacagtg gtttttgata cctatcatat aattgattca aagcaattct
    29041 caaaccctta ggatcaatct gccagcccca ttcacttgat ggaagatacg gatttttaat
    29101 tcctccagtt attaagtttc cttggacccc ttcccaattt tctggatgag cactgactgc
    29161 actagacatg tagtagctaa aagaaacaaa atcaacaggg taggctgcta agatttcttc
    29221 atcacctact tctattttta agttgacacc tttttcttca aacattcgtt gcgtatacgc
    29281 tggataatat cctcttgctt gaacatcact aaagaacaaa gaagttcttt gctgctcaat
    29341 tgttttttga atatcatccg gatcacaaga atacggaaca agaagctgat agctgatcat
    29401 acaaccgact tgtgaacctt tgataatttc atgacaagct tttgtagcta aagcacttgc
    29461 tataaactga tggtgtgcac cttggtattt cgcttgttcc aaatggggat ggttttcttc
    29521 aattactcct acgctaacaa aagggtgatg ttttacacag ttgatctcat tgaaagtcat
    29581 ccaatatttt actttatttt tatagcgttt gaatactgtt tcagcgaaac gaacgtaaaa
    29641 ctgaataagc tttcgattag gccatccacc atattctgta accaaatata acggcatttc
    29701 ataatgggaa agagaaacga ctggctcaat cccatatttt agacattcgt caaacacttg
    29761 atcataaaaa aataatcccg cttcattggg tgtttcttca tcaccatgag gaaaaattct
    29821 tgtccatgcg atagacatcc gatagcattt aaaacccatt tcagcaaata aacggatgtc
    29881 ttctttaaag tgatgataga aatcatttcc gtgtctttta ggataataat acttgctgtt
    29941 gggatcctgc gccgctttta cttgctgatg cgtcatcata tgccattgat ctgtccattt
    30001 ttctttaggt aaatcgctgt cgaatgtata cacatcagac acagataatc ctttttcatc
    30061 tacatcccat gcaccttcac attgattggc cgctgtcgct ccgccccata aaaatccttc
    30121 tggaaatttt ttcatatcga tccccctaaa cttgctagtt ttcttgtttg attatttttc
    30181 ttatgactag taatgatatt tttgaatcta ctactatttg taacaataac cattgtggtc
    30241 ggatcatatc cctctttgat taattcttgc aaatctactt ccatcaataa atcgccttta
    30301 cgtacttttt ttccatcaga tactgcactt tgaaagtatt tgccatttaa attcactgta
    30361 tcaataccga tatggatcag tatctctgct ccttcttcac tgactacccc aattgcatgt
    30421 ttcgttggaa aaactgttga aacttccccg tttacaggac tgtagatttt tccgtccgtt
    30481 ggaatgatcg caaacccttc tcctaaagat ccactagcaa atgttggatc gtttacttca
    30541 gataaatcaa ttatttcacc tgctgcagga gtacttaaca ctatatcctt tgaggattct
    30601 tcttcgattt catccttgta gccgaaaaga aaagttagca tacaggaact gatcatcgct
    30661 aagaaataac tagcaactag ccaaacaaat ccttcaccaa tataaatcgg gaaagacaaa
    30721 atacttcttg tagcaactgc caaagcaccg gctcctgcgg caccagctac tccaccaaat
    30781 atagctccac atatacaagc aatgtaaaat ggtcgtttat atttaagagt cacaccataa
    30841 acagcaggtt cagtgattcc aaataatgct gtgataaatg cagataaaga taaacttttt
    30901 actttcttat ttttcgtttt caaaaatacc ccaagtactg ctcctgcttg cgcagctacg
    30961 gcagtcatac atgctggacc tagcgtatca cgaccgtaga aagaaagatt gttggtcatt
    31021 actggtacaa gtccccaatg taacccaaaa atcactaaca cttgccaaaa tccgccgata
    31081 acagcaccag ataaaacagg gctaaagtta taaacagcta aatatccagc tgcaatcagc
    31141 tgacttgcat aatctgcaat tggacctaca actaataaag taattgggac catcacgatg
    31201 atacaaatga atggtgtcaa taaatttcga atactttcgt gcaagctttt gcgaatctta
    31261 ggctcaatat aagaaagaac ccatacagct aaaattgctg gaataaccgt acttgtatac
    31321 cttgctagaa tcacgggcat ccctagaaat cgcaaggtta tgctatcact atatgcactg
    31381 acaatcgtag gataaacgag agctgctgca ataaccatag cgatgaaacg atctgtattg
    31441 aacttttttg cagcagtata tgccaaaaat ataggcaaga aatagaaaaa tgcatcagct
    31501 gctgcattca aaatgatata agttcctgat tcagtagtta ataaattcaa actcgtacat
    31561 aaggcgagta ctcctttgat caaaccactt cctgccaaga cgcctaaagt cggggcaaaa
    31621 acagcggcga tcatgtctat aaaagagttt aaaatgcctt ttttctcagt tcctctcttt
    31681 ccactagatg catcatcttc tagtttcatt tgtttggata atacgtcata cacttcattt
    31741 actgcatttc ctactacgac ttgaaacatt cctccagctt cgactacagt gatcacacct
    31801 tctagttgct ctaccttctc cttatctgcc ttcttattat cttttagggt aaagcgtaac
    31861 cttgttgcac aatggtaaac attgctgaca ttttcttcgc ctccgacaaa tcggacaata
    31921 tctttcgcta attgtgaata gtccattttt acttccccct tgttaattaa tgccgtagca
    31981 atccttctta caagataagt atagttctta gaaaacggtt tctccattca attagtaccc
    32041 cttataatag cgaacgaaac tgtacaatag tgtgtatggt ttacttagcc gagcaaatca
    32101 ctcactgctt gaaagaaaac aagtatatcg tttctcttat agtcaaagga gacaaaaaaa
    32161 acacgcttga tcttcctttg ctgatgtata agtggtatct caagctgata ctctgtgatt
    32221 tttgtttcta cttggctttt gataagacag cacaacaaag tctcggcatt taacggaagc
    32281 ataactaggt cagaactagg agagattagt tgctgaatgg aatctatctt tttatggatt
    32341 tttatttgat tttcttcttt gtagtaatga tcaagcagtt ttgttttctt tttttcttca
    32401 actatggtca gcttttcacg caaaatctga taaatctccc aagattcaaa ctcatctaga
    32461 acactaggta gataaatcaa atcatcccac tcaaaaataa gctcttgctc agtagtcaaa
    32521 attgcatcat attccgttat tttgtttgat tgatttttga aaagatacac tggttctatc
    32581 cggcattctt tgatccacat gttcatactt tgttgaatct gtttttttag tgtattcaaa
    32641 atactaattg gctggttact aacaatcagt atctcccctt gttttcgaaa aggattcaac
    32701 gcttcggcaa gatacaaaac aaaataagcg ctttcagcta aattgggacg taattgaaaa
    32761 tgtattggga agaaacgccg aattaaataa atttctaaag gataacggga gatgatcttt
    32821 tgtacaaaat gattcatggt attatgtccg gcatccatcc tcgttttcat ttgtttgata
    32881 tgggttagca aaaattctgt ttcatggaac aataacgaaa caggaagatc taaaaaacga
    32941 ataatcgttt gctcaaatgc tcttagattc ttctctattt ctttgttttt ttcatcatta
    33001 atcatcaaat aatttaactc taatagtcga ttttcaattg cttgtttttc ttgattggaa
    33061 aaagaatagg gtagttctga tgataacttt tttgtgaggg tctctaccat cggaataaat
    33121 aatgtatacc tgtttgtttt ttctaaaagt tcttctcttc gaagaatggt caaaacgaga
    33181 taccgagaaa atcgtatgaa gtcctctcct gaaacaatat aattatatga tataagaacc
    33241 tcttttaatg tcttttggac agtcggcatg atattggaaa aaattgtact gatttgttga
    33301 ggcaaccttg cgtgttggat caccgtttct gttccaacaa gagataagaa aattcttttt
    33361 gaattttctt cagcaatgat ttttagtcct tttgtcgacg aaacttccag ttctaattta
    33421 ggattgcgct cgatccaacg caaaatcgtt tttatttgta atgatacggc tgtttttgaa
    33481 agaaagaaaa tgtctgctat cttttgcata ctgacatacc ctttttgaaa caataagtac
    33541 gtcacaatag ttgaagctcg gaaatccatg ctggaataga ttttatgttt cgtcacctct
    33601 tgtatgattt gctgtttagt ttcagattca attgattcta aaaaataacc tgaacgttta
    33661 tttgactgga taatgatttt cttctcgtag atagcaagca tatttttgat ttcagtttgg
    33721 atcgttttct tgttggtacc taaagcttca gctaattctt cgcttgttat ccaatgattc
    33781 ttatttctta atactacaag aatttggagt tgtcgtttat ttaagtccat ttgcttacac
    33841 ctcctctttg ttataaacat acaggaaaaa cctctcaaat gacaatagtc acttgcagag
    33901 gtttaaaaat catttgaaga aagatctata ttcaaggttt ttaccattag actggataat
    33961 ttgtttataa tattcaaaac tatctttttt caatctccta aagcttccgc tcccatcatt
    34021 ttgtctatcc acataaataa agccatatcg cttcctcatt tcccctgttc ctgccgatac
    34081 gatatcgatt ggtccccacc ataagtaacc aatgatttgg caaccatctt ttattgcctc
    34141 agcaatttcc tgaagatgta tttttagata ctcacaacgt tttggatcgt ggattcgatt
    34201 caattcatct ggtgcttcat ctagtccaat gccattttca actatgaata atggtaattc
    34261 ataacggtct gtcaattcat tcatgacata acgaaaccct agaggatcaa ttggccaact
    34321 ccaaggttcg ggagattttt ccttcaaaaa gggattatcg aaaccatttg ctccgccagt
    34381 atctacgcgc atttctgctt cttttttata gacagcgcta cgataataac taaatgcaat
    34441 ataatctgcc gtgttagctt gcaagcactg ttcgtctcca tctaacatta ctggtgcaca
    34501 gtcttcttcc ttccatgtcc tgtaaatata agctggataa tgccctcggc acatgacatc
    34561 ggagaaaaac catgaattgc gtttaaattg cagtgaacca aatacattct ctggatcaca
    34621 gtcatatgca taagtcgcta atgcagaaaa gctacacata atacccatct tagcatttgg
    34681 atcgattttc tttaataatt gagtagcacg ggcgttcgct agaaacatat gatgacaggc
    34741 ttgataaatc actttttttg ttagtgtagc aatgggttca agtttatctc tagcagggaa
    34801 tgtaaccaat ccagcagctg cataaggcat acgaaaagca ttattgatct cattaaaggt
    34861 catccaatat ttcactttcc cttggtagcg ttcaaagacg gtctgcgcat aaaattcaaa
    34921 aaagtcgatt aattttcgat tttgccaccc cccatactct cctaccaatg ctaagggggt
    34981 ttcataatga ctaagagtaa ttactggctc gatatcatat cgatttaatg tattgattag
    35041 tctgtcataa aatacgagac cagcctcatt tggcttctgt tcgtctcctc ttggaaatat
    35101 tcgtgcccat gagatgcttg ttcgaaaagc ttttaaacct aactccttca gtaaacgaat
    35161 atcctcttca aatcgatgat aaaaatcaat cgcttcatga gaaagataat ggagtgattc
    35221 gtctaaagca atctcccaaa tctttttttc ttcattccac tgaatactag gatgctggtt
    35281 catgatcccg ataagcgtat ctgtcacatt aggtaatttg ccgtcttcca accatgcacc
    35341 ttcacattga ttagcagcta ctgctccgcc ccataaaaaa ttttctggaa atatttcctg
    35401 atccataact atctctcctc gctatcaaca atttcttact tgaagtatag ctcgattcat
    35461 caaaacaaga tattctaact atttcggcag aagagaagaa aaaaattgaa tgctactctt
    35521 catttttaat atttatatat aacaaataca taagacgatc cccgaaagaa aacactcgtt
    35581 aaccatctaa gcaaacgatt tattttactt ccaacacaaa tgcttcataa ggtcgtaaat
    35641 caactttgcc gtctggtaac tgtttcagag gataatttga cacaataacc cttaaagctt
    35701 cacgtggaac agtgaacgtc tgcttttcac tggacatatt acaaataacg agccattgct
    35761 ggtcttcata tatgcgagaa taggcaaaga catgctcagg cgtcttttcc actaattcat
    35821 agtctcccca gacgatgatg tcatgttttc tacgcaaatc gatcaatttc ttgtacatat
    35881 aaaaaacaga atcttgatct gctaacgctt cttctgcatt gatttcttta taatttggat
    35941 tgactcgaag ccatggaatt ccagtagtaa acccagcatg ttcacgggcg ttccattgca
    36001 taggcgtcct agcattgtct cttcctttcg cattgattga ttccaagatt tcctcttttg
    36061 taaatccaga agaaatcctt tcgttataca tattgattgt ctcaatatct tttgcttccg
    36121 cgatatcatc gattgggcaa ttcgtcatgc ctaactcttc tccttgatag atataagggg
    36181 tgcctttcat catatggagc aagatagcaa acatcttggc actttctttt cgatacacat
    36241 cgtcatttcc ccaacgagaa acaattcgcg gaagatcgtg attattccaa aataaactat
    36301 tccatccttc atcacctaaa gatgtttgcc atttggataa tacttttttc agttcagaga
    36361 tactcaaggg tttcaaatcc catttgtctt tgccttcttg ctgatctaat cctatgtgtt
    36421 cgaattggaa gaccatggat aattcatttc ttttaggatt ggaataaagt ttagcgattt
    36481 cggtagttgc tccccatgtt tcgccaacag tcatcagatt tttgtcacca aaagttgctt
    36541 gattcatttc ctgaagatac tcatgcagtt taggtccatt acttgtgatt tctttatccg
    36601 gttctttgcc aatcatatcg atcacgtcca tacgaaagcc cccaatacct ttatcgatcc
    36661 agaaattcat catttgataa atctcttggc gaacctgttt atttttccaa ttcaaatcag
    36721 gttgacgctt gctatacaag tgcagaaaat attgccccgt tttttcatcc aactgccaag
    36781 cagaaccact gaatattgag cgcaatctat taggctcttt tccattgacc ggatcacgcc
    36841 aaatataata gtcccgataa ggattttctt tatttttacg ggcctcaatg aaccacggat
    36901 gttcatcaga agtatgattc accaccagat ccatgatgat acggatattt cttttctttg
    36961 cttcttcaat cagttcttcc atttcttcca tcgtaccgaa ttctgccatg attgcttgat
    37021 aattactgat atcataccca ttgtcatcat tcggagactc gtatactgga gaaagccaaa
    37081 ttgcatcaat acctaaatac gccagataat ccaacctgga aataatcccc cgcaaatctc
    37141 caataccatc ttgatttgaa tcttgaaaac ttcttggata gatttgataa acaactgaat
    37201 ggtgccacca tttttgttcc atgattttcc tcctataaat ggatgtttct attttttgct
    37261 taatcattta ttttagtctc tctcactaaa aattgtaacg ctttcaaata gctattgcac
    37321 cttgttatcg gtaacagaat aaaacaaaaa aacagctgag gaaattccct caactgtttt
    37381 ttgaaaggat tgatttatgc agggaagaat gctgatttca caacttcttt ataaacggtg
    37441 ttcactcagc tgtcccttgg tattagctca aaaaacggca aaatatgagg aactattttt
    37501 tcgttttttt ctctaatact caggccagaa cactgatttc acaacctctt tataaacggt
    37561 gttcactcag ctgtccctcg gtattagctc aaaaaacggc aaaatataag aaactatttt
    37621 ttcgtttttt tctctaatac tcaggccaga acgctgattt cacaacctct ttatttcaca
    37681 ttcaatatcc acatgaatcc cacaaataca aagaacagac catacatgat tggagatact
    37741 tcttttcctc ttttagctgc taacatggta attggataga agataaaacc taatgcaatc
    37801 ccatctgaaa tactataagt caaaggcatg ccgatcaaga tcaagaatga agggatcgca
    37861 atctctaagt ttccccattc gatttgtcgc aaagattgtg ccatcaatac tcctaccata
    37921 atcaatgctg gtgcagttac ttgtgaagta acaactgata gtaatggaga aaagaacaag
    37981 cctaacaaga agaaaatccc tgtagtaata gctgtgatac ctgaacgtcc tccaacggcg
    38041 ataccagctg atgattctac aaatgcacca acaggagaag taccaaataa ggaacccgca
    38101 agcatagctg ttgaatcaga agctaaagct ttccctacac gaggcatctt gttgtctttc
    38161 atgaaacctg cttggtttgc taatccaact aaagttcctg ctgtatcaaa gaaagtcaca
    38221 agcaagaatg tcagcactac gacccacatt tgaagagaat tgatgtcccc tacatgtttt
    38281 aatgcaacta aaaatgttgg tttcaaactt ggtgctgcgg caacaatttc agaaggcatt
    38341 ggaatcaaac ctgtgaccag acctaaaatc gttgtagctg ccatcccgat gaagatgcca
    38401 cctggtacac gccgaacaag caagatagca gttacgacta atccaaaaat cgtcaaccaa
    38461 gtagaaccaa cgttgagtga tcctaatcct actaatgtag attcattcga aacgatgatc
    38521 ccaccttcgc tcagaccaag gaaagcgata aatagaccga taccgccaga aatcgcatat
    38581 ttcaaatctg ctgggatcgc atcaatgatc atttcccgca gcttgaagat tgtgatcaaa
    38641 ataaaaatca aagaagcaac gaatacgccg gctaatgctg tttcccatgg aatccccatt
    38701 cctaaacaaa cagaataagc aaaaaaagca ttgatcccaa gagcaggtgc cgttccaatt
    38761 ggataacggg ctaatacccc cattaaaaca cagccgatcg cactggctaa tgcagtcgca
    38821 gtaaacactg ctccttcatc cattccagaa gcacctaata cagttggatt tacaaataag
    38881 atataagcca ttgagataaa tgttgtaaat cctgccagta cctcacgttt taccgtggtg
    38941 ttcaattttt ctatttcgaa gtaggaaagt attttttcct tcatcttcca tccccctaaa
    39001 tccgaacatt ataaataaag tccgttaaaa tttcgattta ttaataaaac aacgtgtttt
    39061 atcatatact aaaacaaaac ataagtaaac aaaaaattcg gattttaagc aaaatccgaa
    39121 cgttccatct gattaaatcc ctttatctca aagttcatga acattcactt tggattacaa
    39181 gaatttcttt attgtgttaa taaagtttct tcattctagt ctcaaaaatc aatagtgtga
    39241 tttgatttta ccatttcgtt tctgttcttc tttgatgaat tcttttgtat cgacaggatt
    39301 cagattattt agtccgccaa aacctgtatc gaccattttt ccccagcctt ttgtcttctt
    39361 ttttcctgat ttgagattct ttgctcgaag cttctcacta tgttttgctc gccgatactt
    39421 caaataacct tctaatcttc ccattagatc cactgttttt cctccttttt attcaatcag
    39481 acgactcagc ttttcctata acaaaataat tgccttcagg gtcagaaaaa ttgaaagtca
    39541 agatgccatt gttatctgtg atttctcctg gttcttccat gttctcgtag atagtttcaa
    39601 attcatccgt aaaaaatact aaagaaggcg gtggtcctaa tacctctggt gaaaacttct
    39661 gcacaaatac ttttgggaat acgcctaatt ctaattcttt tgagatgctg agtacaatac
    39721 tcttaaattc ttctggcaat tcgattgttt cagaaatttc tgcaccaatt ttttctacaa
    39781 agaatcggca aatcatctct acgtcattga cataaagcat cacacgtgtt ttttgtatca
    39841 tcatttgacc cttctttcta tttgattttc tttcctgtct ataaactgat tccattatat
    39901 cacgaaagcg caaacacctt gtgtttgata ctattcaaga aaaataaagc aatagtttct
    39961 aggattgctt tcctccacaa acttttttta caaaaaaaaa aacggcaatg aatgccgctc
    40021 ttcttatttc gattctttta taattctgct tctgattcat ctttggttag tgtgtgtcta
    40081 acactagtta cgccaaatgt ttgatcgatt ttttccttca acgcttcgat tgcctgtgcg
    40141 ccacgtgccg cttgttcagc attgatggat tcaatgacca gcaccgctgc ttgtgtttct
    40201 tccgtcagca ttgttctttg ggcttcacgc cagttgagac agcgacagat cgctccttgt
    40261 tcatcatagt agatgatttc ttccggaagt gccggagcat cggaatctgc acctaaaggc
    40321 aggaatgatt cgccaccttg ggcttttcct aaatgcagat ttcctgcgat cttttcgata
    40381 tcttctccgc cgcaaggtac gccaaaagac aaggaaatac tattatagat atctacaagc
    40441 ggattgattg ggaaaaattc ccgttcttga tttgcacgtt tcagtaaagc ttcaatagaa
    40501 gaacgtgcac cttttttagt cttgaatgtg gtaaatgcct gacgccactg agcaatgact
    40561 tcgttttggc tgaatgtttc ttctgtaagg taatttcttg cttcttttgc tgcctcattc
    40621 aataattgat gatacgtttc ttcactttct tttgttacgt ggttatcgat accatttacg
    40681 gataagattt cgatggttgc ttcaggaaat acttcccaga aattggtatc cactattact
    40741 tttttcatct gttcacacct cttgattaga ataattgctt gatgaataga tagtctgttt
    40801 ggggatcatc tcccatataa aaaacatgtt cgctgtattt gataaatcct tttttacgat
    40861 aaaaagcgag cgcaacatcg ttgtgttccc aaacaccgag ccaaatggct gttttttgct
    40921 tttcttttgc tttttcaata gctgtatcta gtaaaaatgt accgatacct tgatgtttaa
    40981 atttttttcg tacatagatt cgctcgattt ctaaaaaatg atcagccatt tcctcagatt
    41041 gactcgttcc tgtatttagt ttcaggtaac ccgcaggctc ttgatcgata ctcactagga
    41101 aaaaatcggt ttctttatct tttagctctt ctgtaagttt ttctagtgca taagcttgtg
    41161 tcaagtaatc agctaaatgc tccggtgaat tatcttttcc aaatgtatct gtaaatgttt
    41221 cgatactaat ttgttgtagt aagtctacat ctgaaggcgt catttttcga atttcgtatt
    41281 tattcacggc ttcttccttt caatagatcc ttttattgcc ttttttgaca aaattccaat
    41341 cttcgctaac attcttgcat actttattta aaagatattc caattgtttt gcttcttcgt
    41401 cagaaagacc atttaatgcg actgcattgg agtagttgtt ttctcgaatg atgaaaggat
    41461 agatttctgc tcctttttta gtcggataga gtttcttgat ttttttattt tctttatctt
    41521 ccaaacgttc gatcatccca ttggattcta atttcttgat tgctcgggca gtcgttgtac
    41581 gatctacttt gatcatttct gctagttttt cttggatgat tccaggattt tcgcaaatac
    41641 gtactaaata aagatactgt cctcttgtta aatcgacttc tttgaattcg atattgctaa
    41701 tagaatctag cgctcgggca atcattccga tgtctcgaag gatttcagcc atttcatccc
    41761 acctttattt tgtttgtaag actagtttac ctctttttgt tgcaaatgca actattttca
    41821 ataaaaaaat caagttcttt tttaataact agtaaagtat attcttttct tcttttacga
    41881 aaaagctcaa tcacaataga tctggttttt gttttctaaa ataagtaact gtttcttcgt
    41941 tgttcgcttt tctgatagct ttggcaactc ttctatacta aaaaatccca tgttggaagt
    42001 ttcattattc tcaataaact tggcctcttc accgtggatc gcacaagcaa aaacaagttt
    42061 ataatactga aaaagctgtg gaatatcttt ttgtttattc gtatcaaaaa ctgctcgcaa
    42121 ctgagtagtt tctactgtta atcctgtttc ttcatagact tctttacgca cattttcttc
    42181 aggagacaat cctatttcag caaatccacc aggaagtgac cattctttcg tccgtttatc
    42241 ttcgattagt aggaccttgc catctttttt gatataggca cgaacatcta cttttggtgt
    42301 tggatgtcct tctccaatcg ttgtgagcgc ttttatttct tcgaaagttt cttttcctat
    42361 ttcactgatc agttccaatg aaatcgtccg gatctcctca aaacgctctt tatcaaatac
    42421 atcttttcca taatatagac cagcttgaga aagtgcgagt agtcttttgt attgagccaa
    42481 cttcttgttc attctcttcc cttctttttt agcaaatgta tgactatgta atcaagtata
    42541 gaagactggt ttaattacgg caaataaata taatgaaatc tgcaataaat aaaagaatgt
    42601 taaaaaactc gagaacaaaa gacattcgac ttctctgggg ctgtgaataa atggcgttca
    42661 aaagcttgca tctgcggtaa taagccccac caaacgaaaa atatgacgag ctattcttgt
    42721 ctggctgttc ttattaattg aggcaggaca gctttttcac aacctcccca ctaaacggtg
    42781 ttctcctacc aactcctcgg tcacaagtca caatattcat caaacatgag aagctgtttt
    42841 tttgaaattg ctccttgtga ctcggagctg aacgacaggc tcacgacctc tcggtaaaca
    42901 gcgttcaaaa gccaattttt cgatattaag ccatttcttt gttacttatt gtcattgatt
    42961 tctttttcta atttatccaa tagtggagtg taatccactt gagtatagtc tgttacatca
    43021 aattcatata gtgttcctaa ctggaatttg tcatattttc ggtcggcgat gatctttttg
    43081 aatgcttctt tcgtaatatg attggtccct ttcgttcgat cttccaatac gtcaccatag
    43141 tgataatgat ccatgatata gctcaaatga cgatctgatt cacgatcacg tttgtaccga
    43201 cgttgacata acacttcaaa gtctgctgtt aggcgaatcg tgatcacctg atatccgtat
    43261 ctatctgcta attcttggag tcgtgctttt tgcttatcac taaaaggata ctcggacaca
    43321 atcactttct ttcctgcttc catatacaaa tcaagtgctt tgtaatagaa aggataaact
    43381 ttcgtccttt cttgttccgc ccgttcttcc aaattgttaa acccgatgga ttctgcatag
    43441 tattccttga tttcatcgag cgtgatggca tacatatctg gaaatcgttc ccgtagttta
    43501 ttcataagat atgtttttcc tgtaccagga ctaccagcca gaagaatgaa atactttttc
    43561 atctcattta tttcctaagt taattggact ttccaagttc ataaacagtc caatcttatg
    43621 cttgacaatt tcttttactg cttcatttgc tggaatcaca ttatggcgaa gagacttgtt
    43681 gatttctgtt tcactaaagg tttctttagc tactttcgtc cagttgacta aaagctccgt
    43741 tccaacattg aatttgcgga tcccgttatt gatcaattca ggataatctt cttctttcac
    43801 acctgttcct ccatggataa ctagggggac atcaactact gcatcgattt ctttcagcaa
    43861 gggaatgttt acttctgttt ttgatttgaa ttgtccatga ttcgtaccga tagcaatagc
    43921 taatgcatcc acgcctgtag cttctacaaa tttcactgcg tcttccggtc ttgtatatac
    43981 cttgtcattt tcagcgacat ggattccttc ttctgtaccg ccgattgttc caagttctcc
    44041 ttctacggaa acaccgtgcg catgggcata ttctactact gcttttgtct tcaggatatt
    44101 ttctttgaaa ggcagatggg acccatcata catgacagaa gtatatccgc tgtcgattgc
    44161 ttctttgata tcatcaaagt ctcgggcgtg gtctaagtgc aagactgcat caaccatttc
    44221 ttcttctgca atagctgtca ttacattgac taacacatgc atgccgatat atttagcggt
    44281 atcgacactt gtttgaataa tgatcggcga acccatttct tttgctgcgc ctagcatagc
    44341 aggaagcatt tctaggttat gcgtattaaa agctccaact gtaaaattca actcttctgc
    44401 tgtttttgtt acttctttca atgttgtata catcttttat tgccacctta tctttttatt
    44461 tgtttccttt gatcgttact tgttttgcaa tttgtcgcat ctcatccatt ttgcccatgt
    44521 agtaatcgat cccttgatca tcaccagcac gagcagcatc tgcacgcttc tggttataga
    44581 ggctcatcag ttttttatac ccatctccga tcgtctgttt attttccaaa ctcttttcta
    44641 aatattggat ggcttgatcg ttcacataca attcagaaaa tttaagtcca acttgttcat
    44701 aaagtttcgc taatttatgc ttatcttggg tagtggaaat ctcttgtttc aattgtgtaa
    44761 tctctgattg aatcttttcg gtcgcttctg gcgatagggc tttttcttct tttgccagaa
    44821 cttttttcgg ttctttttct tttttcttaa aaaatccaaa catcattttc cctactttca
    44881 attaaaatgc tttcaaaact gggcttaaga tccaggcata taagagtgct gcggctactg
    44941 tatctacatc ggttgctgta gcattgatga atcccatcga attaaagatc gttactagca
    45001 aagcgggcaa taatgtaata aagaaaccat gagcaatacc gccaatgatc gctcctcggc
    45061 gtccgccaac tgcgttgcca aatatccctg ctgtccctcc ggcaaagaaa ttggttagca
    45121 taccaggtaa aatcatcgct aagccgaaca tgggcaaagt aaacatggca ataatcgtac
    45181 caatcgttgt cgtaatgaaa cctaaaatga cagcatttgg gctataagga aagaaaactg
    45241 gacaatctaa ggcagggatc gcatcaggaa ctaatttcat agctatccct cgaaatgccg
    45301 gcacgatttc acctaacagt agacgtacac cagctagcaa tacatacaca cccactacga
    45361 attggatggc ttgtaaaaag gcaaacatga tatagttttg atctcctgat aattcagaag
    45421 cgaatggttc gcctgcaaat aaaacagtga tgatatatag cgggaccatc acgaccatga
    45481 cagacaaata agtatcttgc agaaattcga aatgtgtcgg caacttgata tcttcgatcg
    45541 actttttatt ttcgcctttc tcaccaaaaa gcttagcaac accggcttca aataagtacc
    45601 cgatcgtaca aaaatgacct aaagcaatat catttgatcc agtgatttta cgaatgatcg
    45661 gttgcgctac tgccggcata gcaatggcga aacaagctcc gataaaacca ccgaccaaga
    45721 tcaatacgat ccctcgcaga ccggcaaaat agccaaagac tgtcgtcatc gttgccatcc
    45781 ataaaattgc ttgaccagtt aaaaaaatgt acttccattt tgtgaaacga gccaaaataa
    45841 tattgaatac aaaaatggct aagaacgtca gtgcaatatc tctacctaaa cccaaatcat
    45901 tcattgcttg accattgata gattcaatag aagggatgat cccttgcata tgaaatcctt
    45961 cagtaaagat tttcccgaaa taagttaaac ttccaacgat gatgctcgaa ccggcactca
    46021 acacttgaaa tcctaggagt gtctttaacg tgccagagat gacttgtcct gctgatttct
    46081 tctgcagtag tagacccaac atagcaatca atgcaatggt gatcgatgcc tgagtcaaaa
    46141 tattttcgat gataaaattg ataacgccca tgtttttacc ctcttttctt tttgtattta
    46201 gataactccc ttatcttgca gaactggggt gattttttct tcaatttcat tttttgaaac
    46261 aatatttttc aaatagatca tcgccgtttt aggatcaact gcgaactttt cgaactgcga
    46321 acgaaaattt tctgctgtga tgatcacatc tggtttcatg gaaactgcgg aagaaatatc
    46381 acaatggtcc agtttggctt ctactcctaa tttgttcaaa acatcctcag cagacatctg
    46441 tgctgcaaaa ctacttccta aacctgcacc gcatacgaat aaaatgttca atttgttcat
    46501 ctttgttctc ccccgtatca tttattattt ctcaactact gaaaaaagca gttgtttgaa
    46561 atcttcgata tttgtgcatt caatcaatcg atccaacttc tcttcgttgt tgatcaattc
    46621 aattagttct ttcatgatgt tcagatgcga gtaggagtct actgctgcca aacaaaaaat
    46681 aatctttact ggatccattt caggattacc gaaatcgatt ggttgctcaa tcgtagctac
    46741 gctgactcct aatttattga ccccatcttc tggtcgggca tgggctagtg ccatgtgttt
    46801 gccaatcacg atatacggtc cgtaatcatt cactgcacga atcatcgctt cgacatagcg
    46861 ctcttcgaca atgttttctt taaccaatgg tctagcagct agttcaatcg attcctccca
    46921 agtagtggct ttttctttga tcatgatatt gctgtccgtc aaaatatctt ttaacatagg
    46981 ctggatctcc cttttgttga tttctaaatt gtttgtttcg aataattttt ctaacttgcc
    47041 atagatgatt cgatctactc ggccaccact ttctttcatg acatctatta tttgagtgaa
    47101 taactccgtc gattcatgac tgttaggctc taaccgctgt agttcttcat ttcttttcaa
    47161 aaagtcttct ataaccaaga tattcgtctc agttaaaatc ggatcgacaa ctaaaagcgg
    47221 cttatttcga taattcaatt gaaaggtgct gaaaaccaga ttgacatcca atttatctac
    47281 taattcgatt tcttttgaac ttaaaactgc agtaatctcg atctgaggaa acttttcttt
    47341 cagattctct gctaacagat ttccagtagc catcccatgg ttacagacaa cgactgattt
    47401 ataaacataa ttcaagtctc gcttgattac actggcgatc gtcgaaaagt ggatgacaag
    47461 aaaagctatc tcgtcctcca gtatcttgcc accaattact tcttcgatcg taggaacaaa
    47521 atgagtgact gcgcggtaga ttgggaaata atttgttcgg atgttttctt tcaatggatt
    47581 catgacttgg atatgattct tgattcttac aatcagtgca gccatgtgct tatagagatt
    47641 ttcgaataaa gcttcttcct ttagatggaa aggtacgtgt gtctcgttct ctacaaattg
    47701 gatcaattgg atcgttagta attgagcttg tacccactcg attgaattgg agatatctct
    47761 agcattgaac gtctcaatag tgaaacgaag ataatttctt tctacttgcg ggacattgtc
    47821 caattgaaat tcctcgatca tgtggtcaat aaatgcagaa aagctactgt cttttatttc
    47881 tgtatttttc caattaatgg cagagataaa ttcatgtttc ctgattcgct ggatccagat
    47941 caatgtaaaa agcaggattt gttttttata aatatcatct tcttgtttca acaacttatt
    48001 ggaatagatc tcctgtattt ttttgatttc caccaaagga aaataatgga gaaagatttt
    48061 ttgagccggt gttaattgtg catcttgatt cataaagaag tcggtcattc ccagattctg
    48121 attgataaaa tcatagatca tcgtacggat agatcgttct gaacctttat aaaccagtcc
    48181 ttgctttcca taactgacga tctctatccc ataactgacc agtgttgctc gtaaccgtct
    48241 catatcttca tctaatgtac ttttagagat ttgaaattct tcttcttttt tgtttagatg
    48301 gatcgggatt cggctgtaag caacggctaa aactagatca aaaatacgtt cgcgccgatt
    48361 caaaaagccg tctttcacat cttttatttc agtttctaat cgagcttgct tttccttttc
    48421 cagcttcaac aaaaaaactt tgttgcgaat tgtacggatc tccggaagct ttttactgct
    48481 caaccagata ttcgcttcaa gaacttcctg acggatcgtc cctgactaac tattacacgt
    48541 cttctgttgt gtttgttatt gcaggaaatc ttaatataga aggttttcta tttgtttaca
    48601 ttttgttgaa ttcgaatttt tttggcacat aatttggcac aagtacttgt tttctattat
    48661 tcagtatatt aaattccgcc ttcacaaaaa gaacattcgt tcgtatactc tttttgaggt
    48721 gaacttttat gctgatggaa ggaaa
//
LOCUS       DACECZ010000007        44065 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.007, whole genome shotgun sequence.
ACCESSION   DACECZ010000007 DACECZ010000000
VERSION     DACECZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.007"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(176..1741)
                     /gene="guaA"
                     /locus_tag="IWO35_000440"
     CDS             complement(176..1741)
                     /gene="guaA"
                     /locus_tag="IWO35_000440"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ0315521.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(1800..2723)
                     /locus_tag="IWO35_000441"
     CDS             complement(1800..2723)
                     /locus_tag="IWO35_000441"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ0315522.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            2919..3524
                     /locus_tag="IWO35_000442"
     CDS             2919..3524
                     /locus_tag="IWO35_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0315523.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            3832..4848
                     /locus_tag="IWO35_000443"
     CDS             3832..4848
                     /locus_tag="IWO35_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0315524.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            4989..6290
                     /locus_tag="IWO35_000444"
     CDS             4989..6290
                     /locus_tag="IWO35_000444"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ0315525.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            6336..6998
                     /gene="deoC"
                     /locus_tag="IWO35_000445"
     CDS             6336..6998
                     /gene="deoC"
                     /locus_tag="IWO35_000445"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ0315526.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            7019..7417
                     /locus_tag="IWO35_000446"
     CDS             7019..7417
                     /locus_tag="IWO35_000446"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ0315527.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            7483..8565
                     /locus_tag="IWO35_000447"
     CDS             7483..8565
                     /locus_tag="IWO35_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0315528.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            8777..10339
                     /locus_tag="IWO35_000448"
     CDS             8777..10339
                     /locus_tag="IWO35_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0315529.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            10332..11465
                     /locus_tag="IWO35_000449"
     CDS             10332..11465
                     /locus_tag="IWO35_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0315530.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            11465..12421
                     /locus_tag="IWO35_000450"
     CDS             11465..12421
                     /locus_tag="IWO35_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0315531.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            12520..13686
                     /gene="deoB"
                     /locus_tag="IWO35_000451"
     CDS             12520..13686
                     /gene="deoB"
                     /locus_tag="IWO35_000451"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ0315532.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            13710..14528
                     /locus_tag="IWO35_000452"
     CDS             13710..14528
                     /locus_tag="IWO35_000452"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0315533.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            14545..15252
                     /gene="deoD"
                     /locus_tag="IWO35_000453"
     CDS             14545..15252
                     /gene="deoD"
                     /locus_tag="IWO35_000453"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0315534.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(15550..16236)
                     /gene="gpmA"
                     /locus_tag="IWO35_000454"
     CDS             complement(15550..16236)
                     /gene="gpmA"
                     /locus_tag="IWO35_000454"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0315535.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(16408..16989)
                     /locus_tag="IWO35_000455"
     CDS             complement(16408..16989)
                     /locus_tag="IWO35_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0315536.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(17122..17805)
                     /gene="rpiA"
                     /locus_tag="IWO35_000456"
     CDS             complement(17122..17805)
                     /gene="rpiA"
                     /locus_tag="IWO35_000456"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ0315537.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            18124..18537
                     /gene="rpsL"
                     /locus_tag="IWO35_000457"
     CDS             18124..18537
                     /gene="rpsL"
                     /locus_tag="IWO35_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ0315538.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            18621..19091
                     /gene="rpsG"
                     /locus_tag="IWO35_000458"
     CDS             18621..19091
                     /gene="rpsG"
                     /locus_tag="IWO35_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ0315539.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            19185..21272
                     /gene="fusA"
                     /locus_tag="IWO35_000459"
     CDS             19185..21272
                     /gene="fusA"
                     /locus_tag="IWO35_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0315540.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            21444..22631
                     /gene="tuf"
                     /locus_tag="IWO35_000460"
     CDS             21444..22631
                     /gene="tuf"
                     /locus_tag="IWO35_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0315541.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            23038..23346
                     /gene="rpsJ"
                     /locus_tag="IWO35_000461"
     CDS             23038..23346
                     /gene="rpsJ"
                     /locus_tag="IWO35_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ0315542.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            23377..24006
                     /gene="rplC"
                     /locus_tag="IWO35_000462"
     CDS             23377..24006
                     /gene="rplC"
                     /locus_tag="IWO35_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ0315543.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            24034..24657
                     /gene="rplD"
                     /locus_tag="IWO35_000463"
     CDS             24034..24657
                     /gene="rplD"
                     /locus_tag="IWO35_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ0315544.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            24657..24947
                     /gene="rplW"
                     /locus_tag="IWO35_000464"
     CDS             24657..24947
                     /gene="rplW"
                     /locus_tag="IWO35_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ0315545.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            24986..25819
                     /gene="rplB"
                     /locus_tag="IWO35_000465"
     CDS             24986..25819
                     /gene="rplB"
                     /locus_tag="IWO35_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ0315546.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            25862..26140
                     /gene="rpsS"
                     /locus_tag="IWO35_000466"
     CDS             25862..26140
                     /gene="rpsS"
                     /locus_tag="IWO35_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ0315547.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            26163..26510
                     /gene="rplV"
                     /locus_tag="IWO35_000467"
     CDS             26163..26510
                     /gene="rplV"
                     /locus_tag="IWO35_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ0315548.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            26524..27180
                     /gene="rpsC"
                     /locus_tag="IWO35_000468"
     CDS             26524..27180
                     /gene="rpsC"
                     /locus_tag="IWO35_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ0315549.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            27183..27617
                     /gene="rplP"
                     /locus_tag="IWO35_000469"
     CDS             27183..27617
                     /gene="rplP"
                     /locus_tag="IWO35_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ0315550.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            27607..27795
                     /gene="rpmC"
                     /locus_tag="IWO35_000470"
     CDS             27607..27795
                     /gene="rpmC"
                     /locus_tag="IWO35_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ0315551.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            27820..28086
                     /gene="rpsQ"
                     /locus_tag="IWO35_000471"
     CDS             27820..28086
                     /gene="rpsQ"
                     /locus_tag="IWO35_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ0315552.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            28144..28512
                     /gene="rplN"
                     /locus_tag="IWO35_000472"
     CDS             28144..28512
                     /gene="rplN"
                     /locus_tag="IWO35_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ0315553.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            28549..28857
                     /gene="rplX"
                     /locus_tag="IWO35_000473"
     CDS             28549..28857
                     /gene="rplX"
                     /locus_tag="IWO35_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ0315554.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            28885..29424
                     /gene="rplE"
                     /locus_tag="IWO35_000474"
     CDS             28885..29424
                     /gene="rplE"
                     /locus_tag="IWO35_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ0315555.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            29443..29628
                     /locus_tag="IWO35_000475"
     CDS             29443..29628
                     /locus_tag="IWO35_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ0315556.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            29664..30062
                     /gene="rpsH"
                     /locus_tag="IWO35_000476"
     CDS             29664..30062
                     /gene="rpsH"
                     /locus_tag="IWO35_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ0315557.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            30094..30630
                     /gene="rplF"
                     /locus_tag="IWO35_000477"
     CDS             30094..30630
                     /gene="rplF"
                     /locus_tag="IWO35_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ0315558.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            30791..31147
                     /gene="rplR"
                     /locus_tag="IWO35_000478"
     CDS             30791..31147
                     /gene="rplR"
                     /locus_tag="IWO35_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ0315559.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            31168..31668
                     /gene="rpsE"
                     /locus_tag="IWO35_000479"
     CDS             31168..31668
                     /gene="rpsE"
                     /locus_tag="IWO35_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ0315560.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            31683..31862
                     /gene="rpmD"
                     /locus_tag="IWO35_000480"
     CDS             31683..31862
                     /gene="rpmD"
                     /locus_tag="IWO35_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ0315561.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            31906..32346
                     /gene="rplO"
                     /locus_tag="IWO35_000481"
     CDS             31906..32346
                     /gene="rplO"
                     /locus_tag="IWO35_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ0315562.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            32346..33641
                     /gene="secY"
                     /locus_tag="IWO35_000482"
     CDS             32346..33641
                     /gene="secY"
                     /locus_tag="IWO35_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ0315563.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            33703..34350
                     /locus_tag="IWO35_000483"
     CDS             33703..34350
                     /locus_tag="IWO35_000483"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ0315564.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            34541..34759
                     /gene="infA"
                     /locus_tag="IWO35_000484"
     CDS             34541..34759
                     /gene="infA"
                     /locus_tag="IWO35_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ0315565.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            34792..34908
                     /gene="rpmJ"
                     /locus_tag="IWO35_000485"
     CDS             34792..34908
                     /gene="rpmJ"
                     /locus_tag="IWO35_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ0315566.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            34926..35291
                     /gene="rpsM"
                     /locus_tag="IWO35_000486"
     CDS             34926..35291
                     /gene="rpsM"
                     /locus_tag="IWO35_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ0315567.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            35319..35708
                     /gene="rpsK"
                     /locus_tag="IWO35_000487"
     CDS             35319..35708
                     /gene="rpsK"
                     /locus_tag="IWO35_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ0315568.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            35788..36726
                     /locus_tag="IWO35_000488"
     CDS             35788..36726
                     /locus_tag="IWO35_000488"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ0315569.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            36754..37134
                     /gene="rplQ"
                     /locus_tag="IWO35_000489"
     CDS             36754..37134
                     /gene="rplQ"
                     /locus_tag="IWO35_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ0315570.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            37406..38245
                     /locus_tag="IWO35_000490"
     CDS             37406..38245
                     /locus_tag="IWO35_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0315571.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            38221..39090
                     /locus_tag="IWO35_000491"
     CDS             38221..39090
                     /locus_tag="IWO35_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0315572.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            39080..39877
                     /locus_tag="IWO35_000492"
     CDS             39080..39877
                     /locus_tag="IWO35_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0315573.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            40080..40826
                     /gene="truA"
                     /locus_tag="IWO35_000493"
     CDS             40080..40826
                     /gene="truA"
                     /locus_tag="IWO35_000493"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0315574.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(41281..43950)
                     /locus_tag="IWO35_000494"
     CDS             complement(41281..43950)
                     /locus_tag="IWO35_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0315575.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT        14121 a         8160 c         9552 g        12232 t
ORIGIN      
        1 caataggaaa ataaaggtag tgaataaaag caaagccctt gttagtagtt aatctaacaa
       61 gggctttggt agggaaaatg gtaggaaacc atcttatttt ttacgaaaat caggaacttt
      121 agtaattgaa taatgttgat ttataggtat tcctcatcca tattatgttg gctctttatt
      181 cccactccac tgttgctggt ggtttagaag tgatgtcata cacgatacgg ttgacgtggg
      241 ctacttcgtt tacgatacgt acagagattt tttgcaagac ttcccatggg atacgggcaa
      301 agtcagcggt cattccgtcg attgaagtta cagcacggat accgactgtg tagtcgtaag
      361 tacgtccgtc acccataaca ccgacagagc ggatccctgg aagaacagtg aaatattgcc
      421 agatatcacg gtcaagtcca gcagctgcga tttcttcacg caagatagca tcagattcgc
      481 gtacgatttc tagcttgtct tcagtgattt cgccaagaac acgaatacca agaccaggac
      541 cagggaatgg ctgacgccaa acgattgaat caggcatgcc taactgagtc cctaattcac
      601 ggacttcgtc tttgaataat gtgtttaatg gttcgatcaa ttcaaattgc atatcttctg
      661 gcagtccacc tacattatgg tgagatttga tcgtctgtgc tgtttctgta ccagattcga
      721 taacgtcagt atacaacgtt ccttgtgcta ggaaagagac gccttcttca ccagcaagtt
      781 ttgttgcttc gtcatcaaaa acatatacga attcattccc aatgatttta cgtttttgtt
      841 ctgggtcaga tacacctttc aacttgtcta agaagcgatc tcttgcattg actttgatga
      901 tgttcaagcc gaattttccg cctagacttt ccattacttg ttccgcttcg cctttgcgta
      961 gcagaccgtg gtcaacaaag atagatgtca attggtcacc gatcgctttt tgtaaaagaa
     1021 ccccaacgac actagaatca acgccacctg aaagaccaag caagactttt ttgtcaccaa
     1081 cttgttcacg gattttttct acttgcatat cgataaagtt atccattgtc cagttacctt
     1141 cacagtgaca aacatcaaag gcaaagtggc gcagcaattc gattccgtat tcggaatgac
     1201 gtacttctgc atggaattgg ataccataaa aattacgtgt tgtgtcttgg atagaagcaa
     1261 tcggacaatc tttgcttgtt ccgactgttt caaagccttc aggaacttta gttaccaagt
     1321 caccatggct catccatact gtttgttgtt ttggtgtttc tttaaacaag acagcttgat
     1381 cgcttgttac ttctaacaag gctttcccat attcgcggtt tttagcaggc tctacggaac
     1441 cgccaagttt gtacgtgatc aattgcatac catagcaaat ccctaataca ggaataccta
     1501 aattgaagat ctcaggatcg atcccaaatg cattctcatc atatacactg tttggtccgc
     1561 cagaaagaat gattcccttt ggattcatcg cttttacttc ttctgcagta atgcggtggc
     1621 tcaataattc ggaaaagact ccaagatcac gaatacgtct tgtgatcaat tggttatact
     1681 gactaccgta gtctaatacg ataatctttt caacgttcgt catatcggca acgtttgtca
     1741 aatttattac ccctatccct ttaaatttta tctcaaaata gtcaaactat tttaaaatgc
     1801 taatattttc gcatgaaaat cgaatcgatg atatgatttt cagttttatg gagaattata
     1861 tcggctcttc ctcgtgtagg caagatatat tcctgtaaat ttttcaaatt cacatttttc
     1921 catacatctt ttgccatttt aaaggcttct tcacgatctc cgatagcaaa acgataataa
     1981 taattctctg gattttggaa agccgtatcc agtaacgcac caaaacgatc caaataccat
     2041 ttttcgatca gcacgggatc ggcatcgaca tagacagaaa aatcaaaaaa gtcgctgacg
     2101 tagatttgct gattcgcagg taattgaagc gtattgatcc cctcaacgat caagatatca
     2161 ggctgctgga tcaattcgta cttgtctgga atgatatcat acacatcatg tgagtaaaca
     2221 ggtactttta tctcttcctg accagatttt acacggttca agaaatcgat cagcatctcc
     2281 atatcataac tttcaggaaa tcctttacgg tccatgattc cacgctcttt taatatacga
     2341 tttggataaa gaaatccgtc agtcgtgatc agctgaacgt ttcttcgttt gaaggtacga
     2401 gacaaaatcg tctgcagcag tctggctgtt gtactttttc caacggctac acttccggcg
     2461 atcccgataa taaaaggcgg tacttctacg tatttatgaa gaaataaacc tttactcaag
     2521 aacaaagatt caaattcttt catataaaga taaatcagat gagcaagagg tacatagata
     2581 tctcgaacat cctgcatgga aatgcggtca ttcaaacttt tgatattatc tagttcatct
     2641 tgtgtcaaag gtgcttgccc attacgataa aattctcgcc attcttctct tgccacacgg
     2701 tagtagttca ttggatcatt catattagcc tcctgcgttc tcccatcaat ggaactattt
     2761 taacacaaaa taaacgcaaa ccccaccact taagagagat tgccgatctt tttgtataat
     2821 ctttataata gaaagaaacg gagtcttatt cttgacttgc gcacggatac ataaaaggat
     2881 agactgttgt catgaaagaa atgagaagga tggaagacat gacaaatcat tactattctg
     2941 aacgcccaga tacggcgcac gattttgaac aatgggcttt tgagttaaaa ggcagaacct
     3001 ttcaatttgt aacagattct ggtgtatttt cacgtgaaac agtggattat ggttcacgtg
     3061 tcctgattga tgctttcgat tgggaagagt tacctgatga aggaaagatc ttagatgtgg
     3121 gatgcgggta tggaccgatt gggttagctt tggcatttgc gacacagcgg tttgttgaaa
     3181 tggtcgacat taatgaacga gcggtaagtc tagcgcaagg aaacgcaaag cgtaaccaaa
     3241 tcgaacatgt tgatatccat caatcgaata tctatgaagc agttcatgaa gaaacctatg
     3301 cagctatcgt aagcaatccg ccgatccgtg ctggaaaaaa agttgtacat gaaattttga
     3361 caggcgcata tcctcggttg aagaagggcg gtacactgac aattgtgatc cagaagaaac
     3421 aaggagcgcc gagtgcgcaa aagaaaatgg aagaaacatt tggtaatgca gaaatcgtca
     3481 caaaagataa aggatattat atcatcaaaa gtgtgaaaga ataactagct gaaaaatagt
     3541 ggagcgacga aagagtggaa ctgggcgtgc atgtacgggt acgtatcagt tctttttttg
     3601 ttgagaagtt tttaaaaaca aagaactatt ttttcttcaa aaagaaatag taggaacaga
     3661 gtcgttgttt tttacgataa aataaataag atacagaact tgtatagaag gctgccaagt
     3721 cggtagataa aggaggtttt tatcaaaaaa catactttcc ttttttctct attcttttct
     3781 tgggggtttt ggtacactga tagggaattt agatagaagg gagccaatag gatgaagctg
     3841 accatcagag atatcgcaga aatggcaggc gtatcagtaa caactgtctc acagattttg
     3901 aataacaaag gaagcagatt tagtgataag acaagaaaaa aagtgttgtc gatcgtgaat
     3961 gaacatcatt ataagcccga ttatttcgct tcaaatgtga tcaatcgaca ttcaaagacc
     4021 atcggaatga tcgtccctga tgtcactgat ttcttcttct ccaaactgat cgaaggtgta
     4081 gagagctatt tgaatccttt gggctatgtg atcatgttat gcaattctcg tcatagccaa
     4141 gaaaatgaga tcaagtattt acaagagctt tcccaccgct cagtggacgg aatcttgctg
     4201 gccacaccaa atattttacc tgagaaatat gcattagaca gcggttttta tcaaaaaatg
     4261 ccaatcattt taatcgatcg cggattgaat cgtcgggata acggacggct tatcgtaaag
     4321 gaatatgagg gcgcctatca agctgtttcg ctgtttattg aaaatggcca tacgaagatc
     4381 ggcatgctga aggaaaccac tggatactat caattagaag aacgtttcaa cggatatcgt
     4441 catgcgctga aagatcatgg cctgcctttt aatgggaaat ttgttgaaca tggagagttg
     4501 actgtccaag gtggatatac tgcatcaaaa aaattactca aacataaaga tatcacggct
     4561 attttttgcg ggaatgatgc catggcgatc ggatgctatc aggcgatcga tgaattgggc
     4621 aaaaaaatcc cagaagatat ctcagtcata ggattcgatg gattaaagct gtcagaatac
     4681 atgattccgc aattgacgac agtgcagcag ccaagttttg atattggatt ttatgctgca
     4741 cgtttcctga tcgatacgat cgaatttcct caaagaaaag ttccgaacaa agtgttcgaa
     4801 acaaaattaa ttattcgtga gagcgtaaaa gtattgacaa agggctaact cggcggtata
     4861 ttgcttatgt aagcatttac aaaggctagt tagtgctttt tttattttta gtaaaccagt
     4921 ttactaaagt ggtttacctt tcctaaaaat aatgtataat accgaatgaa gacaaggagg
     4981 atcaggcaat gcgaatggtg gatctgattg aaaaaaagag ggatggaaac gagctgtcga
     5041 aagaggaaat cgagtacatc gtcacgaact atacgaatgg aaagattcca gattatcaag
     5101 tcagtgcatt attgatggca attttttatc aagacatgac aaatgaagaa atcacgaact
     5161 taacattggc gattgctaat tctggtgatg tcatcgattt gagttctctt gaaggaataa
     5221 aggtagataa gcattcgaca ggtggcgttg gtgacacgac cacattgatt ttagcccccc
     5281 tagtagctag tgtcggagtg acagtggcta aaatgtctgg acgcggtcta ggttacacag
     5341 gtggtacact agacaagcta gaagcaattc ccggatttca gatcgagctt tcggatgaag
     5401 aatttgtacg cattgttaat gaaagtaaag tagcggtaat tggtcaatca ggaaacttgg
     5461 caccggcgga caaaaaattg tatgccttgc gtgacgtcac agcgaccgtt gattctcttc
     5521 cgctgattgc cagttcgatt atgagtaaaa aaatcgcggc aggagcagat gcaatcgttt
     5581 tggatgtgac cactggagac ggtgctttca tgaagaacat cgaagatgca agacgtctgg
     5641 caaagaccat gacgagcatt gggaaactgg ctaaccgaga aacagtggcg gttatctcag
     5701 atatgtcaga acctcttggc gaagctatcg gaaatagtct agaagttgtc gaggcaatcg
     5761 aaacattaca aggaaatggt ccagaagatc tagtagaaat gtgctacgcc ttaggaagcc
     5821 aaatggttgt tcttgctggc aaagctaaga caaccgatga agcaagaacg ttgcttcaag
     5881 aagctctgga atcaggtaaa gcgttagcaa aattcaaaga aatgatccaa aatcaagggg
     5941 gagacccagc gatcgtggaa catccagaac gaattttgac tgcacgctac acaatggagc
     6001 ttcctgctaa acaaagcgga gttgtgtcaa aaatcgtggc aaatgaatta ggaattgctg
     6061 caatgatgct aggtgctggg cgtaaaacca aagaagaaga tattgatcac gctgttggat
     6121 tgaaactgca taaaaaaatt ggtgacactg tgacaaaagg tgagtcacta ttgacgatct
     6181 atagtaacga cgaagaaatc acttctgtca ttgaattatt atataaaaat attgaaattg
     6241 gtgaatcggc aatgaagcca acgttgattc atgacatcat cacagaataa aggaaaaaag
     6301 cggactttac cgttttaaag aaaaggggaa aagaaatgga actaaaccga atgatcgacc
     6361 atactatttt gaaagcagac gcatctaaag aagctgtgat gcaaattatc gaagaagcaa
     6421 aaaaatacca tttttattct gtctgcatca atccagcatg ggttgcgcta gcgaaagaac
     6481 aattacaagg tgagccagta gccgtatgta cagtaatcgg gtttcctcta ggagcaaaca
     6541 cacccgagac aaaagcattt gaaacaacag atgccatcaa taacggtgca gatgaagtcg
     6601 atatggtcat caatatcggt gctttgaaat caaaggatga tcaacaagtc caaaaagata
     6661 tcgaagcagt tgttgaagca gcaaaagatc gtgcgcttgt caaagttatt attgaaacat
     6721 cgttattaga tagagaagaa atcatccgag cttgcgaaat agcaaaagct gcaggcgctg
     6781 attttgtcaa aacatcaact ggtttctcaa caggcggagc aaaggtagag gatgtccgtt
     6841 tgatgcgtga aactgtagga ccagaaatgg gtgtcaaagc ttccggcggt atccataacg
     6901 aagaagaagc aatggcaatg atcgaagctg gtgctacaag aatcggaaca agcgcaggag
     6961 tagcaatcgt atctggttca actggtgaag gctattaatt ttttcaaagg agaagctcat
     7021 ggcagaactc aaacaagaat ggatcaacac agctattgaa gcgttagata aagcctatgt
     7081 tccttattcc cattttccgg taggcgcttg tctcgtaacg gaatcaggca agatttatca
     7141 aggcatcaac attgagaatg catcatttgg attaacgaat tgtgccgagc gcacagcttt
     7201 tttcaaagca gtatctgaag gagaaaaaac atttacgcat ctagtcgtag cggggcacac
     7261 ccccgatcct atctctcctt gtggtgcatg tcgacaagta atggcagaat tctgttcccc
     7321 tgatatgccg gttacgctag taggagacaa tggtgtgacg aaggcaacga ccgttcgtgc
     7381 actgctgcct tatgcattta ctgaaaaaga tctataactc atgttcattc agtcagctga
     7441 ctgttgaaaa ataaaacaat tattttagga ggctttacag aaatgaaaaa agcaaaatta
     7501 tttggtttag gtgctgtcgc actagcagca gggttatttt taggtgcatg cggcaacaac
     7561 ggctctactg attcatccgg tggcaaaagc tcatcagaca caacaactgc agcattgatc
     7621 acagacacag gtggtgtaga cgaccgttca ttcaaccagt cagcttggga aggactagaa
     7681 aaatggggga aagatcaagg actttcacga ggaaatgatg gcttccaata tttccaatct
     7741 tcaaatgaat cagattacat cccaaatatt gaccaagcat tgaatgctgg attcaaaacg
     7801 atttttggta ttggttataa actaaaacca gcgatccaag aacaagcaac aaacaataca
     7861 ggtacgaact ttgttattat cgatgatgtc atcgatggat tagataacgt tgtatctgca
     7921 actttcaaag acaacgaagc ttcttatctt gcaggtgttg cagcagctta tacgactgaa
     7981 acaaacgtag tcggctttat cggcggtgta aaaggtgaag taatcgatcg ttttgatgct
     8041 ggattcaaag ccggtgttga tgcaggtgcg aaagaattag gaaaagaaat caaagtattg
     8101 aatcaatatg ctggtgactt ctctgctcca gataaaggac gttcaattgc tcaaggtatg
     8161 tatgcacaaa acgcagatat catcttccat gcttcaggcg gtacaggtaa cggtgtattc
     8221 caagaagcaa aatcattgaa cgaatcaggc gacaaaaaag tttgggttat cggtgttgac
     8281 cgcgaccaat cagacgaagg cgaatacaca ttaaacggcg aaaagaaaaa cttcacattg
     8341 acatctacac tgaaagcagt tggaacagta gtagaagatt tggcacaaaa atcagcggac
     8401 ggtaaattcc ctggcggcga acacactgtt tatggattga aagaagacgg tgttggtcta
     8461 acagaaggac aactttctga cgaagcgaaa aaagcagttg atgaagcaaa agaaaaaatc
     8521 atttcaggtg acgtaaaagt tccagaaact cctgaagaaa actaaaacca tttctatagc
     8581 cttgttagac acatgtttga caaggctatg gtttgattag aacaagaatt gttcatttat
     8641 ccaagctgcc ttgtgacagt ttggataagg gaacaactcc ctgaagtaat gtttgtttca
     8701 aaagaagaac gctcactaag cgtgaatctt agtcaatcgt tgccctagat agaaatcgtt
     8761 aggatgtgaa aaaaaggtgg ttcaagaaaa tattgtcata gaaatgcaga atattaccaa
     8821 agaatttgga acattcaagg caaatgacaa catcaattta caagtaaagg caggagaaat
     8881 tcatgcgttg cttggagaaa acggtgctgg caaatctaca ttgatgaacg tgctttccgg
     8941 attattagag ccgacatcag ggaaaatttt gatgcgtggg aaagaagtaa agatcacaag
     9001 tccgaccaaa gccaaccaat tagggattgg gatggtccat cagcacttta tgcttgttga
     9061 tgcctttact gtaacagaaa acatcgtgtt gggaagcgag cctagtcgcg cagggatgct
     9121 tgaccataaa aaagcacgaa aagagatcca aaaagtttct gaacagtatg gattgtcagt
     9181 caacccagat gcttacgttc gtgatatttc agttggaatg gaacaacggg tagaaatttt
     9241 aaaaacactt taccgaggag cagatgtact gatttttgat gagccgacag ctgtattgac
     9301 ccctcaagaa attgatgaat taatcgtgat catgaaggaa ttagtcaaag aaggcaagtc
     9361 gatcattttg attacgcata agttagatga aatcaaagca gtagctgacc gctgtacagt
     9421 tatccgccgt ggaaaaggaa tcggtacagt caacgttaaa gacgtaacct cacaacaatt
     9481 agccgatatg atggttggaa gaacggtttc attcaaaacg atgaaaaaag aagcgaagcc
     9541 tcaagaagtc gttttgtcta ttgaaaatct agtggtgaaa gaaaatcgtg gattagaagc
     9601 cgtgaaaaac ttgaacttag aggttcgtgc tggcgaagta cttggtatcg ctggaatcga
     9661 tggaaacggg cagtcggagt tgatccaagc tttgactggt ttgcgaaagg cagaaagcgg
     9721 acatatcaag ctaaaagggg aagacatcac taataaaaaa ccccgaaaaa tcactgaaca
     9781 tggtgtagga cacgtgccag aagaccgtca taaatacggg ttagtcctag atatgacatt
     9841 atctgaaaat atcgcactac aaacctatca tcaaaaacct tacagtaaaa acggcatgct
     9901 gaattattca gtaatgaatg aacatgccag agaattgatc gaagaatatg atgttcgaac
     9961 aacgaatgaa cttgttcccg caaaagcttt atcaggcgga aatcagcaaa aagcaattat
    10021 cgctcgtgaa gtcgaccgag atcctgatct gttgattgtt gcaaatccaa ctcgtggact
    10081 ggatgtaggt gcgatcgaat ttattcataa acgtctgatc gaacaaaggg acaaatacaa
    10141 agcagtgtta ctgatcagtt tcgaattaga agaaatttta aatgtttcgg atcgtattgc
    10201 tgttatccat gaaggagaaa ttgtcggtat cgttgatccg aaagaaacat ctgaaaatga
    10261 attaggtcta ttgatggcgg gctatacctt agaagaagca cgaaaagagt tagaaaaggg
    10321 ggctggcgca catgaatgat cgcagcgaaa aattccgtaa tttattagtg cctgtacttt
    10381 cagttgtttt aggattgatc ttaggcgcga tattgatggc tgcttttggt tttcagccca
    10441 tccaaggtta ttcttctatg ctagatgcag cccttggttc gcaaagaggg ataggtgaaa
    10501 cgttaaggca agcaacgccg ttgattttta cagcattagg tttttctgta gctaactcag
    10561 ctgggttttt caatattggg ctttctggac aagcactttg cgggtggatc gtcagtgtct
    10621 gggtggcact tgcgtttcca gatctgccaa aagttgtttt gcttccgtta tgcgtgatct
    10681 taggtgcctt agctggtgcg atggcagcgg ctgttccagg tttacttcga gcattttttg
    10741 gaacaagtga agtgatcgta acaatcatga tgaactatat cttattatac gtaagcacat
    10801 ttgctttaca tgaagtgatg cctgaaagct atcgctcaag tttggactct tccaatatga
    10861 tcagtcaaaa tgcctcactg cggttaccat ggttaacaca agcttttggc ggctctcgcg
    10921 taaactcagg attattttta gcaatcatcg cgcttatttt cgtttggctg ctgatgaaga
    10981 aaacgacttt aggatttgaa atccgttctg ttggattgaa tccttttgct tctgagtatg
    11041 caggaatgag tagtaaacgt acgatcgttt tgtcgatggt catttctggc gctttggcag
    11101 ggcttggcgg tgtggttgaa ggattaggaa cgtatcaaaa cttctttgtt caaacgactt
    11161 cgctatctat tggatttgac ggaatggctg tttccttgct tggttctgga tcagcaatcg
    11221 gtattctttt atcagcgatc ttgttcagta ttttgaagat cggtggactt ggcatgcaga
    11281 caggagcagg tgttccgttt gagatcgtca atgtatcgat tgctttgatt atcttctttg
    11341 tcgggatcaa cttcctgatt cgtttcgtca tggctaaagt cttccaaggg aaaaaacaag
    11401 aagagatcgt tgcgacaata gaatcgaaac caacgaatca aaaccatgaa gggggcgaac
    11461 tataatgtcg actatcgaat taattgcctt gatccttact tctacactgg tttattcaac
    11521 accgctaatt ctaacgtcac taggcggaac tttttctgaa cgaagcggga tcgtaaacgt
    11581 agggctagag ggaatcatgg tcatgggtgc ttttagttcc atcgttttca acttgacgat
    11641 gagtccatca cttggttcta tgacaccatg gatcgctatt ttagtgggtg gcgtcgtagg
    11701 tatcctattt tctctgattc acgcagtagc aacagtcagt ttgcgtgctg accatattat
    11761 cagcgggaca gtaattaatc tgatggcacc tgctttaggt gttttcttga tcaaagcatg
    11821 gtatggtaaa ggtcaaactg ataacatcac gcagaatctg gggtattttt ctttcccagg
    11881 attagcagat attccggtca ttggaccaat tttctttaaa aatacattct tacctgctta
    11941 ttgtgctatt ttgattgctg tcctagcttg ggtgatcatc ttcaagacga aattcggctt
    12001 gcgcctccgt tcagttggtg aaaatcctca agctgccgac acattaggta tcaatgtata
    12061 tgctatgcgt tattcaggcg tactgatttc tggcttgtta ggcggtatgg gcggtgctgt
    12121 ttttgctcag tcgatttccg gtaacttttc agctggtacg atcgttggac aagggttcat
    12181 ctccatggct gctatgatct ttggtaagtg gaatccttta ggggcaatgg gtgcctcatt
    12241 gttctttggt tttgctcaaa gcttgagtat cattggcgca caattaccag ttatttcttc
    12301 gattccgcca gtattgctgc aaattgctcc atatttgctg acaatcattg tgctggttgt
    12361 tttcttaggg aaagcttctg gtccaaaagc aaacgggaag aactatatca aatcaaaata
    12421 atcttttagc gaataaaaag ggtgggttac gggaagtgtt tttcgtctcg ccctttttgt
    12481 tttaaaggca ataaaaataa aaagaaagaa ggaataatca tgtttaaacg cgtacatctg
    12541 atcgtcatgg attcagtagg aattggagaa gcaccagatg ccgataagtt cggtgatgtt
    12601 ggttctgata cattaggaca cattgcgaaa gaagcaggat tgaacgttcc acatttagag
    12661 cagcttggtt taggaacgat ccgtcctctt gaaggtgtgg aatcagtgaa agatcatcaa
    12721 ggctatgcga cgaaattaga agaagtttct gtagggaaag atacgatgac aggccactgg
    12781 gaaatcatgg gattgaatat caaaactcca tttcgtgtgt ttcctaatgg attccctgat
    12841 gatctgatca aacaaatcga agaattttct ggccgtaaag tcgtatgtaa caaaccttat
    12901 agcggaacag ctgttattga cgattacggt gaacaccaaa tgaaaacagg cgatttaatt
    12961 gtatatactt ctgccgatcc tgtccttcag atcgcggcac atgaagaaat cattccttta
    13021 gaagaattat atcggatttg ccaattcgtt cgtgatataa caaaagatga tccgtatatg
    13081 attggccgaa tcattgcacg tccgtacgtg ggtgaaccag gagacttcac acgaacaagc
    13141 aatcgccatg attatgcgtt gaatccattc ggacacacag tccttgattc tttgaaagaa
    13201 gcaggaaagg acgtgattgc tgttggaaag atcaacgata ttttcaacgg acaaggaatc
    13261 actgaatctg tccgcacgaa aagcaatatg gatggcgttg accagctgct gaaagttatg
    13321 aaaaacgatt tttcaggaat cagctttacc aatttagtag attttgatgc attatacggt
    13381 catcgtcggg atgtagtcgg ctatgcgcat gcgattgaag aatttgactt acgaattcca
    13441 gaattgcttg aagcgatgga agaagacgac ttgctaatga ttacagccga tcacggaaac
    13501 gatccgacgt ttactggtac cgatcatacg cgagaatatg taccgttatt ggtttacagt
    13561 aaaaaaatga aagaacaagg ggcattgcct caaggatatt attcagatat ttcagcaacg
    13621 attgctgaga actttagtgt ttcttcgact gaaaatggcg aaagcttctt gaaattatta
    13681 aaataatctt tttaaatagg agaaagcaaa tgaccaagtt aagcgagatg ttacaagaaa
    13741 cgacccagtt tatccttgat aaaggagtaa aagaaattga ttttggtttg attctaggtt
    13801 caggattagg tgaattagca gatgaaatcg aagatgctgt aatgatttct tatgatgaga
    13861 tcccgttctt tcctgtttcg acagtagttg ggcatgcagg aaagctagtt tatgggtctc
    13921 tggcaggcaa gaaagtgtta gccatgcaag ggagattcca ctactatgaa ggtcattcca
    13981 tgcaaacagt gacctatcca gtacgtgtga tggccggact aggggcccat tcagtcattg
    14041 tgactaatgc atgtggtggt gtcaacgaat catttgtacc aggagatttg atgctgatta
    14101 ccgatcatat caattttact ggtgaaaatc ctttgatcgg tccgaatgaa gatgcaatgg
    14161 gccctagatt tccagatatg agtcaagctt atacgcctga ctatcaagca acagcaaaag
    14221 cagcagcaga aaaactaggt ttggatctta aagaaggcgt atatatgggc tattctggac
    14281 caacttatga aacgcctgcg gagatccgaa tgtctcggac aataggagcc gatgcagtag
    14341 ggatgtcgac tgttccagaa gtcatcgtag cagctcacag cggattaaaa gtgttaggaa
    14401 tctcttgtat taccaactta gcagcaggta tgcaagcgaa tctgagccat gaagaagttg
    14461 tagaaacaac tcagagagtc aaacaatctt ttaaagcatt ggttaaagaa acattgacat
    14521 tattgtaata ggaggaacaa taaaatgagt gtgcatattg aagcgaaaga aggcgagatc
    14581 gccgataaga ttttacttcc gggagatcct ttgcgagcta aatatattgc ggagactttt
    14641 ttggaagatc cagtttgtta caatcaggta cgcggtatgt taggctttac cggcaaatat
    14701 aaaggagaac gtatttctgt tcaaggaaca ggaatgggaa tgccatcagc caccatctat
    14761 gcccatgaat tgatccagtc gtatggtgta aagaaattga ttcgagtagg tacatgcggt
    14821 gcattgtcaa aagacgtcca tgttcgtgat ttagtactag cacaaggtgc agcgaccagt
    14881 tcttcgatga tcgaaaaaaa tttccaagcc tttcattttc ctccaatcag tgatttcaat
    14941 ctattactga aagcatatga gatagctaaa gaaaagggct atacggttca tgtaggaaat
    15001 gttctttcgg aagattcttt ctacaaagat gatttgacag agacgttcca attagctgaa
    15061 cttggcgttt taggagtgga aatggaagca gcggctcttt attacttagg cgcgaaatac
    15121 catgtgcaga cattaagttt gatgaccgtt agcgaccatt tgatcaccgg tgaagaaaca
    15181 acagctgcag aacgtcaatc tacgttcaat gaaatgatcg aagtaggatt ggaaacagcg
    15241 atagcagaat aagaaggaaa tgaaaaagct gccctgcatt aagtaataag aacatccaag
    15301 caaaaatagc tcctcatgtt tttgacttgg ctggacttat tattgcaaat gtaagctttt
    15361 gaacaccgtt taccaagagg ttgtgaatct gccgtttaac tctgagtcac aaggaacaat
    15421 ttcaaaaaac agcttttcat gtttgatgaa tattgtgact tgtgaccgag aagttggcag
    15481 gagaaaaccg tttatacgaa aaaagggact gtgacaaatc gtgtcacagt ccctttttta
    15541 tagtgttttt tataggtagt attttttagc aactgttaag tcatcattta attcataaac
    15601 aagtggttga cctgttggaa tttcaagatc catgatgtct tcgtcagaga taccttcgat
    15661 atgtttagct agggcgcgta atgagttacc atgtgcagct actaaaacag ttttgttgtc
    15721 tagtaatgct ggagcaattt catcttgcca gaatggcaaa gcgcgttcta atgtcacttt
    15781 caagttttct ccaccaggaa tatcgcgttt gtctaacatt gcgtaacgac ggtcatttgc
    15841 tgcagaacct tcgtcagttt cttccattaa tggaggaaga gtatcgtaag aacgacgcca
    15901 gatgtgcact tgttcgtcgc cgtatttttc agctgtttct tttttgttca aaccttgtaa
    15961 tttaccatag tgacgttcgt ttaagcgcca tgatttgatt tgtggtaccc aaagttgatc
    16021 tgaatattct aagatcaagt tacatgtttt gattgcacga gttaaaacag aagtgtaagc
    16081 aacatcaaat tcgattccag cttctttgat tttgcgtccg ccttcttttg cttcttcgat
    16141 tccttctggt gctagattta catcagccca accagtaaat tggttcaatg cattccattc
    16201 actaagaccg tgacgagaaa aaactaattt tggcatgttt agctctcctt ttaagatagg
    16261 tttatatttc tgaaattttc agttctgttt tattgtacaa ttttttggaa gagatttcca
    16321 tcgaaaaatt agataagatt ctatgagaaa acaagtagag aatataaaaa aacgaacagt
    16381 gcccacgcaa ctgttcgttt ttaggtatta gatgcataat tgctcgattt ctttttcagg
    16441 taaattgata tatttataac atgttccaac tgaaacaccg cattttgaag aaataaattg
    16501 gatcgtttct tttttttcat aatagagacg tcggattttt ttgatcgttt ttgcatcgat
    16561 ttttggtctt ccgccgattt ttcctttttt acgagcttca tctaatccaa ctaatgtacg
    16621 ttctttgatc aatgcgcact ccatgtttgc taggttttcc attaattgga aatagatttt
    16681 tcccatttcg cgcgtatcga ttccttcatc aagactaatc aagtgcagcc cgcgatcttt
    16741 gaataattgc gtgagttctg ttaattgccg cgtagatttg cctaatcgat gaagttggca
    16801 aatgactagt gcatcgcctt gttgcatgtc agcgatcact gactccaaat gaacagcagt
    16861 agaatcattt tgttccactt catatgtctc atggaaaatt tcttgacaac caaagtcaga
    16921 taattgatca acctgagttg ttaaatcaac gtcatttatg gttgtgcgtg cataaccaat
    16981 aattttcatg gtgattcctt tctagcagtg attttctaac tggatataac agtaaaaatg
    17041 tagcataaag tacgtcaaaa agatagtttt ttcgcaaatt ttccgctgtc tttttttatt
    17101 ggatgatcaa gcagtttttc tttatttacg acggattgcg tcaagaatct caacgccttt
    17161 ttctgagccg acaactactt tagaaaccat gtttaggaaa agcccatgtt ctactacccc
    17221 aacaagttca tctaaatagg tagctaagct ttctggctgt tcgatgactt ctagatgcag
    17281 atcaatgatg taatgaccac catctgtggt taagacttct ccgttttcgt ctgtacgcaa
    17341 gacgggttgg aatctttttt cttcaaaaat atgaagtagc tgttgggagc cataagggat
    17401 cacttcaact gggagtggga acttcccaag tttatcgact aatttagaag aatcaacgat
    17461 ccaaatcgtt tcttttgagt acgtagcgac tattttctcg aaaagcaaag cagcgccgcc
    17521 gcctttgatc ccttgaaaat cagcactgat ttcatcagca ccgtctattg tcaaatcgac
    17581 tacggggact tcatcaatac tctttaacgg tattcctaat ttttccgctt gttcttttgt
    17641 cgcatttgaa gtagtaactc ctgtgatacg taaaccttct tcacgcatac ggcgaccaat
    17701 ctcttctacc atataatagg cggttgaacc ggtcccgaga cctacgatca tcccgtcctt
    17761 tacgaatttc gcggcttcaa tgccagctaa ttgttttaaa ttcatctttg cgtccacctt
    17821 tttttagttt gagcatcgtg ctttcctcac tattttagcg tgatttacgg taaaaagata
    17881 ccattctttt ttattccaat aaagcttttt caaaaaaaat tgcatctctt gttgacatgt
    17941 tttttcattc gtgatattct aacaaaggtg ctttatctac aggtttctgt gaagaaacgt
    18001 gctgactgtt tatcaaagca tatttgataa ggatgcagac attgcattta ttttttatca
    18061 caaaaaaaga accacctgga tgtgtggaac tagacgcaaa tcgtgggaag gaggaaacaa
    18121 ggaatgccta caattaacca attagtacgt aaacctcgta aatcaaaggt ggaaaaatct
    18181 aattcacctg ctttgaacaa aggatataac agctttaaga aaactcaaac taacgtaaac
    18241 tcacctcaaa aacgtggggt agcaactcgt gtgggtacaa tgacacctaa aaaaccgaac
    18301 tcagctttac gtaaatatgc acgtgttcgt ttgtcaaact taattgaagt tactgcttat
    18361 atcccaggga ttggtcataa cttacaagaa cacagtgttg tattattacg cggtggtcgt
    18421 gtaaaagact taccaggggt acgttaccat atcgttcgcg gtgctttaga tactgctggt
    18481 gttaacgacc gtaaacaaag ccgttctaaa tacggaacta aaaaacctaa agcttaattt
    18541 taattattaa acaaatgaaa cttgcttcta ctatatagaa gtaattacta tgaacaatat
    18601 ttcggaagga ggagttacgg atgccacgta aaggtcctgt tgcaaaacgt gatgttttac
    18661 cagatcctat ttataactca aaattagtaa ctcgcttgat caaccgtgta atggttgacg
    18721 gaaaacgcgg gattgcagct aacattatct ataatgcctt tgacttgatc aaagaatcta
    18781 caggtaacga tccattagaa gtgtttgaac aagcaatgaa aaacgttcaa cctgttcttg
    18841 aagttaaagc acgtcgtgtt gggggttcaa actaccaagt accagttgaa gttcgccctg
    18901 aacgccgcgt tactctaggt cttcgttggg tagtaaacta cgctcgcttg cgcggtgaac
    18961 acacaatgga agaacgctta gcaaaagaaa tcatggatgc tgcaaacaac acaggtgctt
    19021 ctgttaaaaa acgtgaagac actcataaaa tggctgatgc caaccgtgcg tttgctcact
    19081 atcgctggta agattctctc tgtttggtcg gcttttataa gtcgacctca gatagtgtcc
    19141 ttatttttag caactagata gtaaagagag gagaaacaag gagaatgacc agagaatttt
    19201 ctttacaaaa cactcgtaat atcggtatca tggcccacat cgatgctggt aaaacgactg
    19261 ctactgaacg tatcttgtac tatactggta aaatccataa aattggtgaa actcatgaag
    19321 gagcttcaca aatggactgg atggaacaag aacaagaacg tggtattacg atcacttctg
    19381 ctgctactac agcacaatgg aagggtcacc gaatcaacat catcgacaca ccaggacacg
    19441 tagacttcac agttgaagtt gaacgttcat tgcgtgtact tgatggtgct gtaactgttc
    19501 ttgatgcaca atcaggtgtt gaacctcaaa ctgaaacagt ttggcgtcaa gcaacaactt
    19561 atggtgtacc tcgtatcgta tttgcaaaca aaatggacaa gatcggtgct gacttcttat
    19621 attcagtatc aactttacat gaccgtttgc aagcaaacgc tcacccaatc caattaccaa
    19681 ttggtgcgga agacgacttc acaggaatta tcgaccttgt aacaatgaag gctgaaatgt
    19741 atacaaacga tcttggaaca gagatcgaag aaacagaaat tcctgaagaa taccgcgaat
    19801 tagctgaaga atggcgcgaa aaattggttg aagctgtagc tgaaactgat gaagaactta
    19861 ctttgaaata cttagaaggt gaagaaatca ctgaagctga gttaaaagaa ggtatccgcc
    19921 gcgcaacagt taacgtagaa ttctacccag tactatgtgg ttcagcattt aagaacaaag
    19981 gggttcaatt actattggac gctgttcttg attacttgcc atcaccgtta gatatcccag
    20041 ctatcaaagg gattgaccct aaaactgatg aagaagtaga acgtcctgca gacgacagcg
    20101 ctcctttctc agctttagca tttaaagtta tgactgaccc attcgtaggt cgtttaactt
    20161 tcttccgtgt gtactctggt gttttacaat caggttcata cgtacaaaac gcaacaaaag
    20221 gcaaacgtga acgtgttggt cgtatcctac agatgcatgc caactctcgt tcagaaatct
    20281 ctgaagttta cgctggagat atcgctgctg ctgttggttt gaaagacaca actactgggg
    20341 acacactatg tgatgaaaag agtctagtta ttcttgaatc catggaattc ccagaaccag
    20401 ttatccaagt cgctattgaa cctaaatcaa aagctgacca agataaaatg ggtgtggctt
    20461 tacaaaaact atctgaagaa gatcctactt tccgtgctga aacaaacgtg gaaacaggcg
    20521 aaacaatcat cgctggtatg ggtgaacttc acttagatat catcgttgac cgtatgcgtc
    20581 gtgaattcaa agttgaagct aacgtaggtg cgcctcaagt atcttaccgt gaaactttcc
    20641 gtgctggcac tcaagccgaa ggtaagtttg tacgtcagtc tggtggtaaa ggtcaatacg
    20701 gtcacgtatg gattgaattt acaccaaatg aagaaggtgc tggtttcgaa tttgaaaatg
    20761 cgatcgtcgg tggggttgtt cctcgtgaat acattccagc tgttgaaact ggtttgaaag
    20821 atgctatgga aaatggtgtc ttagctggct atccattagt agatatcaaa gctaaacttt
    20881 acgatggttc ataccatgat gtcgattcaa atgaaactgc cttccgtgtt gctgcttcta
    20941 tggcattacg tgcagcagct aagaaagcta atccagtaat ccttgaacca atcatggctg
    21001 tagaagttgt tatccctgaa gattatttag gtgatgttat gggtcacgta actgctcgtc
    21061 gtggacgtgt tgaaggtatg gaagctcgcg ctggcggcca acaagttgtt cgtgcgatgg
    21121 ttccacttgc tgaaatgttt ggttatgcta caactcttcg ttctgctact caaggtcgtg
    21181 gtacatttac aatgacattt gaccactacg aagatgtacc aaaatcagta caagaagaaa
    21241 tcattaagaa aaacggcgga aaagctgaat aatttcttga aaccggatag tcctgcaaag
    21301 agattatcca aagagactat atttttaata tagtttcgtg taaaataatt atcgatgata
    21361 tgggcatgtg gtgtaccttg tgtacatcat atacctatca gacaaataaa aaatatattg
    21421 tatttttagg aggacatttt aaaatggcaa aagaaaaatt tgaccgttct aaaccacacg
    21481 ttaacattgg tactatcgga cacgttgacc atggtaaaac tacattaaca gctgcaatca
    21541 caactgtact atctaagaaa aacggcggcc aagctatggc ttacgatcaa atcgatggtg
    21601 ctccagaaga acgcgaacgc ggaatcacaa tctcaacagc tcacgttgag tatgaaacag
    21661 atacacgcca ctacgctcac gttgactgcc caggacacgc ggattacgtt aaaaacatga
    21721 tcactggtgc tgctcaaatg gacggagcta tcttggtagt ttctgctgct gacggcccaa
    21781 tgcctcaaac tcgtgaacac atcctattgt ctcgtcaagt tggtgttcct tacatcgttg
    21841 tattcttgaa caaagtagac atggttgatg acgaagaatt actagaatta gttgaaatgg
    21901 aagttcgtga cctattaaca gaatacgaat tccctggtga cgatgttcct gtagttgctg
    21961 gatcagcttt gaaagctcta gaaggcgacg cttcatacga agaaaaaatt cttgaattaa
    22021 tggctgcagt tgacgaatac atcccaactc cagaacgtga caacgacaaa ccattcatga
    22081 tgccagttga agacgtgttc tcaattactg gacgtggtac tgttgctaca ggtcgtgttg
    22141 aacgtggaca agttcgcgtt ggtgacgaag ttgaagttgt tggtattgct gaagaaactt
    22201 caaaaacaac agttactggt gttgaaatgt tccgtaaatt gttagactac gctgaagctg
    22261 gagacaacat tggtgcttta ctacgtggtg ttgcacgtga agacatccaa cgtggacaag
    22321 ttttagctaa accaggtaca atcacacctc atacaaaatt ctctgcagaa gtatacgtgt
    22381 tgacaaaaga agaaggtgga cgtcatactc cattcttcac taactaccgt ccacaattct
    22441 acttccgtac aactgacgta acaggtgttg ttgaattacc agaaggaact gaaatggtta
    22501 tgcctggcga caacgtaaca atggaagttg aattaatcca cccaatcgct atcgaaaacg
    22561 gtactaaatt ctcaatccgt gaaggcggac gtacagttgg tgccggtgtc gttacagaaa
    22621 tcaaagctta attcacttaa gttttaaagt aaagcaacat ttattcggac gtaatcgctc
    22681 ggaacgaagg tcttaggatc tttgttccga gcttttttat ttattgcagg atcaggaaaa
    22741 aagacaggag tattataaat agaagaaaca cgaaataaaa gagtcagtat tttctaaatg
    22801 aaactttatg aaaacaaaat gttttttaca agaaaaagcc ttgctttgtg agtttatata
    22861 tattataatg acaaaggtgc gtttacttta gtgaattgca cagacgtaat cagaatgatt
    22921 tcgtcggcta agaaacgaga ggttgcgaca cgcccggacg ctttgccacg aacgagcgtg
    22981 acggaaattt tcgtggagct atgtctactt ttcaaaatag gcgaaggagg gaacaagatg
    23041 gcaaaacaaa aaattcgtat ccgtttaaaa gcgtatgaac accgtatttt agatcaatca
    23101 gcggataaaa tcgtggaaac tgcaaaaaga actggagcta gcgtgtctgg tccaattcca
    23161 ttaccaactg aacgcagtct ttacacaatc atccgtgcga ctcataaata caaagattca
    23221 cgcgaacaat tcgaaatgcg tacacacaaa cgtcttatcg acattgtgaa cccaacacca
    23281 aaaactgttg atgctttgat gaagctagac ttaccatctg gtgtaaacat cgaaatcaaa
    23341 ttataaaact aaaacaaaaa taatggaggt gtactcatga ccaaaggaat cttagggaaa
    23401 aaagtgggaa tgacacaaat cttcactgaa tctggtgaat taattccagt aactgttgta
    23461 gaagctactc caaacgtagt actacaagtg aaaacaatgg aaacggatgg ctacgaagct
    23521 attcaagttg gttaccaaga tatgcgtgaa gttttatcaa acaaacctgc gaaaggtcat
    23581 gttgcaaaag caaacacggc tcctaagcgc ttcattcgtg aattcaaaaa tgttgagcta
    23641 ggagaatatg aagtaggaaa agaaattaca gtagatgttt tccaagcagg agacattatt
    23701 gatgtaacag gtactacgaa aggtaaagga ttccaagggg ttatcaaacg ccacggacaa
    23761 agccgcggac caatggctca cggttctcgt taccatcgtc gtcctgggtc aatgggtcca
    23821 gttgcaccta accgtgtatt taaaaacaaa cgtcttgctg gccgtatggg tggcaaccgc
    23881 gtaactatcc aaaacttgga agttgtacgt gtcgatgcag aaagaaatgt tatcttgatc
    23941 aaaggtaatg ttcctggagc taaaaaatct ttaattacta tcaaatcagc tgtgaaagct
    24001 aaataattgt aggaagaaag gaggaactaa ggaatgccga atgtagcatt attcaaacaa
    24061 gatggaagcc aaaacggtga aatcacactt aacgaagaaa tcttcggaat tgaacctaat
    24121 gaaagtgttg tttacgatgc aatcgtaatg caacgtgcgt cattaagaca aggaacacac
    24181 gcggttaaaa accgtagcgc tgttcgcggc ggcggacgta aaccatggcg tcaaaaagga
    24241 acaggtcgtg cacgtcaagg atctatccgc tcaccacaat ggcgtggagg tggcgtagtc
    24301 tttggaccaa caccacgttc ttacagctac aaacttccta aaaaagttcg tcgtttagca
    24361 atgaaatctg tattgtcaga aaaagttgct gaaaacaact tggtagcaat cgaaggcttg
    24421 aacttcgacg caccaaaaac aaaagaattc aaacaagttc ttgctaactt atctatcgac
    24481 tctaaagtat tggttgttct agaaaaaggt aacgactttg ctgctttatc agcacgtaac
    24541 ttaccaaacg tttctgtagt aacttctgat aatgtgagtg tgctagacgt tgtatcaaat
    24601 acaaaagtgt tagcaacaca aactgctctt actcaaattg aggaggtgct tgcataatga
    24661 acttactaga cgtaatcaaa cgcccagtga tcactgaaaa atcaatgctt gccatggatg
    24721 acaagaaata cacttttgaa gtggacactc gcgcaaacaa aactttagta aaacaagcgg
    24781 tagaagctgc ttttgacgtt aaagtcaaaa acgtgaacat cgtgaacgta cgtgcgaaat
    24841 tcaaacgtat gggcaaatat gcaggatata caaaaaaacg tcgcaaagcg atcgttacat
    24901 tgactgaaga ttcaaaagaa atccaattat tcgaagctgc tgaataagca ctaatagata
    24961 actcattaaa ttaggaggga aaaacgtggc gattaaaaag tacaaaccta cctcaaatgg
    25021 tcgtcgtaac atgacaggtt ctgattttgc tgaaatcaca acatcaacac ctgaaaaatc
    25081 attgttacaa ccattgaaaa accatgctgg acgtaacaac aacggccgca tcactgtacg
    25141 tcatcaaggt ggcggacaca aacgccaata tcgcgtgatc gacttcaaac gtaacaaaga
    25201 taacgttgtt gcaactgttc aaacgatcga atatgatcca aaccgttcag ctaacatcgc
    25261 gttagtacac tatgaagatg gagtgaaagc ttacatctta gcacctaaag gactagaagt
    25321 gggtatgaaa cttgtttcag gtccagaagc agatatcaaa gtcggaaatg ctcttccatt
    25381 agaaaacatt ccagttggta ctgttatcca caacattgaa atgaaacctg gtaaaggtgg
    25441 acaattgatc cgttctgctg gtacaagtgc tcaagtactt ggtaaagaag gcaaatacgt
    25501 attgatccgc ttgaactctg gtgaagttcg tatgatctta gctgcatgcc gtgctacaat
    25561 cggttctgta ggtaacgaac aacacgaatt aatcaacatc ggtaaagctg gacgctctcg
    25621 ttggatgcgt aaacgcccaa ctgtacgtgg tagcgtaatg aaccctaatg atcacccaca
    25681 cggtggtggt gaaggtaaag ctcctatcgg acgtaaagca ccagtttctc cttggggtca
    25741 accagctctt ggttacaaaa ctcgtaacaa aaaagctaaa tcagacaaac ttattgttcg
    25801 tcgtcgcaaa aataaataat tactttgacc atgtgtcgca cattttgaga ggaggttcac
    25861 catgggtcgt agtttaaaga aaggaccttt tgtcgatgag catttaatga aaaaagttga
    25921 agctcaagca ggggttgaaa agaaaaaagt tatcaaaact tggtcacgcc gttcaacagt
    25981 tttcccaagt tttgttggat ataccatcgc agtttatgat ggacgtaaac atgtaccagt
    26041 ttacatccaa gaagacatgg taggacacaa attaggtgaa tttgcaccta caagaactta
    26101 tcgtggacat gctgctgacg ataagaaaac gaaacgctaa tttgagggga ggataaatcc
    26161 aaatggcaga acaaatcaca tcagctaaag caactgcaaa aacagttcgc acttcacctc
    26221 gaaaagcccg tttagtaatt gaccttatca gaggtaaaag tgtcgcagat gcaatttcaa
    26281 tcttgaaatt cacaccaaac aaatctgctg gaatcattga aaaagtattg atgtcagcag
    26341 ttgcgaatgc agaaaataac tttgacttag atgttgaaaa cttggtagta agcgaagctt
    26401 ttgttaacga aggacctaca atgaaacgtt tccgtcctcg tgcaaaagga tcagcttctc
    26461 caatcaacaa acgtacaagt catatcacgg tagtcgtatc agaaaaataa ggagggataa
    26521 tcagtgggtc aaaaagtaca tccaattgga atgcgtgtag gcatcatccg cgactgggat
    26581 gctaaatggt atgctgaaaa agagtatgct gagttcttac acgaagattt aagaatccgt
    26641 aaatttattt caactaaact tgctgatgct gctgtatcaa cagttgaaat cgaacgcgca
    26701 acaggtcgcg taaacatttc aattcacaca gctaaaccag gtatggttat tggtaaaggc
    26761 ggttctgaag tcgaaagcct aagaaaagaa ttaaacaaat taactggtaa aagaatccat
    26821 atcaatatcg tggaaatcaa aaaaccagat ctagatgcaa aattagtagg tgaaggaatc
    26881 gcacgtcaat tagaaaaccg tgttgctttc cgtcgcgctc aaaaacaagc catccaacgc
    26941 tcaatgcgtg ctggcgctca agggatcaaa actcaagtat ctggtcgtct aaacggagca
    27001 gatatcgctc gttcagaagg atactcagaa ggaactgttc ctcttcatac tttacgtgcg
    27061 gacattgatt acgcatggga agaagcagat acaacatacg gaaaactagg agttaaagtg
    27121 tggatctatc gtggagaaat tcttccaact aaaaaaaaca ctgagaaagg agggaaataa
    27181 tcatgttagt acctaaacgt gtaaaacacc gtcgtgaatt ccgcggaaaa atgcgcgggg
    27241 aagctaaagg cggaaaagaa gtagcattcg gtgaatacgg tttgcaagct gttgattcac
    27301 attggatcac aaaccgccaa atcgaagctg ctcgtatcgc aatgactcgt tacatgaaac
    27361 gtggtgggaa agtatggatt aaaattttcc ctcacaaatc ttatactgcc aaagcaattg
    27421 gggtacgtat gggttctggt aaaggggcac ctgaaggatg ggttgcacca gtaaaacgtg
    27481 gtaaaatcat gtttgaaatc gcaggcgttc ctgaagaagt agctcgtgaa gcgttacgtc
    27541 tagcttctca caaattacca atgaaaacta agatcgtaaa acgtgaggaa atgggtggtg
    27601 aatcgaatga aggttaaaga aatcagagaa ttaaccactg ccgaaatgct tgatcaagaa
    27661 aaacaattaa aagaagaatt gtttaatctt agattccaat tagcaacagg tcaattagaa
    27721 aacactgcac gtattaaaga agtacgtaaa tcgattgcac gcatcaaaac tgtgttgcgt
    27781 gaacaagcta agtaattatg gaaggaggcc attaagcgta tgactgaagg aagaaatcaa
    27841 cgcaaagttt atcaaggtcg cgtggtttca gacaaaatgg ataaaacaat taccgttgct
    27901 gtcgaaacaa agaaaaacca ccctatctat ggcaaacgca tgaactattc taagaaatac
    27961 aaagtgcatg atgaaaacaa cacagcaaaa gttggcgaca tcgtgagaat tatggaaact
    28021 cgtccattat cagctacaaa acgtttccgt ttgttggaag tagtcgaaga agcagttatt
    28081 atctaataac acgagatttt cctatataga ttgaatgcaa aatctgaaag gaggatacac
    28141 aacgtgatcc aagcagaaag tcgtttaaaa gtcgctgata actcaggcgc acgtgaaatt
    28201 ttgacgatca aagtccttgg tggatctggt cgtaaaactg ctaacatcgg tgatgtgatc
    28261 gttgctacgg ttaaacaagc aacgccaggt ggggttgtca aaaaaggtga cgtcgttaaa
    28321 gccgttatcg ttcgaacaaa atcaggagct cgtcgtacag acggttcata tattaaattt
    28381 gatgaaaatg ctgcagtgat tattcgtgat gataaaagcc ctcgtggaac acgtatcttt
    28441 gggccagttg cacgtgaact acgtgaaaac aacttcatga agatcgtttc actagcacca
    28501 gaagttttat aatcttgaga cacttatcaa aggaggtgcg aaacagttat gtttgttaaa
    28561 aaaggcgata aagttaaagt tatcactgga aaagacaaaa acaaagaagg cgttgtacta
    28621 gcagcgttcc cgaaaaaaga taaagttatt gtcgaaggtg taaatgtcgt taaaaaacac
    28681 caaaaacctt ctcaagctgc tcctcaaggt ggaattgttg agatggaagc gccaattcat
    28741 gtttctaacg tgatggtggt tgactctact ggtgtagctg gccgagttgg ctacaaagaa
    28801 gtagacggca aaaaagtccg tgtttctaaa aaaaccggtg aagttttaga taaataaaag
    28861 tttaaggaag gagggtctta ctgaatgaac cgcctaaaag aaaaatatct taaagaagta
    28921 actccatcat tgatggaaaa atttaactat agctctgtta tgcaaacacc taaagttgaa
    28981 aaaatcgtca tcaacatggg tgttggtgat gcggtatcaa acgctaaaaa cttagataaa
    29041 gcagttgaag aattaacttt gatcactggt caaaaaccaa tgattacaaa agctaaaaaa
    29101 tcaatcgctg gattccgttt acgtgaagga atgccaattg gtgcaaaagt tacattgcgc
    29161 ggagaaagaa tgtacgaatt cttagacaaa ttggtttctg tttcactacc acgtgtacgt
    29221 gacttccatg gtgtaagtaa aaaagccttt gacggtcgtg gaaactatac tttaggtatt
    29281 aaagaacaat tgatcttccc agaagtagat tatgatttag tagataaagt acgtggtatg
    29341 gacatcgtta ttgtaacaac agcaaactct gatgaagaat ctcgtgagtt gttgacacaa
    29401 ttaggcatgc cattccaaaa ataattaaag gaggcgaact acgtggctaa aaaatcaatg
    29461 attgctaaaa acaaacgtcc tgcaaaattt tcaacacaag cttatactcg ttgcgaacgt
    29521 tgcggacgtc cacattcagt ttatcgtaag ttccatcttt gccgtatttg cttccgcgaa
    29581 cttgcctata aaggtcaaat tcccggcgtg aagaaagcta gctggtaaaa agtaaattcg
    29641 cataagaagg aggtaaaaga tcaatggtca tgacagatcc aattgcagat tttctaactc
    29701 gtatccgtaa cgcaaacatg gttaaacatg agtctttaga agtacctgca tcaaaaatta
    29761 aacgtgacat cgctgaaatc ttgaaacgtg aaggtttcgt tcgtgatgtt gaatacatcg
    29821 aagatgacaa acaaggcgtg atccgtgttt tcctaaaatt tggtaaaaac ggcgaacgtg
    29881 ttatcacaaa cttaaaacgt atctctaaac caggtttgcg tgcttatgtc aaagctgacg
    29941 aagtaccaaa agtattgaac ggtttaggta tcgcaatcat ttcgacttct gaaggtgttg
    30001 tcactgataa agaagctcgt gaaaaaaata tcggcggcga agttatcgct tacgtatggt
    30061 aatcaagtag aatctacaag gaggtgtctt taagtgagcc gtatcggaaa taaagtcgtt
    30121 gttatccctg aaggagtaac agttactcaa gacggaaaca acgttacagt taaaggacca
    30181 aaaggtgaat tgactcgtac tttttcagca gatatcaaaa tgaatatcga aggaaacgaa
    30241 gtaactttca ctcgtccaaa cgacagtaaa gaaatgaaaa caattcatgg aacaactcgt
    30301 gcgaacttca acaacatggt tgtaggtgtc agcgaaggtt tccaaaaagc gttagaacta
    30361 atcggggttg ggtaccgtgc acaattacaa ggatcaaaac ttgtattgaa cgttggttac
    30421 tcacatccag tagaaatcac accaccagct ggagtaactg ttgaagtacc agctaacaca
    30481 caagtaattg ttaaaggtgc gaacaaagaa gaagttggcg aattagctgc aaacattcgt
    30541 ggaactcgtc ctccagaacc ttataaaggt aaaggtattc gttatgttgg cgaatatgta
    30601 cgccgtaaag aaggtaaaac tggtaaataa tcgtttagaa aaattgcata gaagccagca
    30661 gcaggaaaaa ggcatttggc tgaagtttac cttcaaaaaa atacattttt acttgtgccg
    30721 cagcttctat ttttcgaaac gtatttactg cttgaaatag tagtacaata aaaataaaga
    30781 ggtgacaatt gtgattacaa aaccagataa gaacaaaaca cgtcaaaaga gacatcgtcg
    30841 tgtacgtaac aatatctctg gtactgctga gcgcccacgc ttgaacgttt ttcgttctaa
    30901 caaaaacatc tacgctcaaa ttattgatga cgtagcgggt gtaacgctag caagtgcctc
    30961 taccttggat aaagaaattt caggtggaac aaaaacagaa gcagcacaag ctgtcggtaa
    31021 actagttgct gaacgtgcag ctgaaaaagg cattaaagta gtagtctttg accgtggtgg
    31081 atacctttac catggccgtg tgcaagcttt agctgaagct gctcgcgaaa atggactaga
    31141 attttagaag aaggaggaat accattcatg gtttatatcg atccaaaaca tttggaatta
    31201 gaagaccgcg ttgttgcaat taaccgtgta acaaaagttg ttaaaggtgg acgtcgtcta
    31261 cgttttgctg ctttagttgt tgtcggtgac aaaaacggac acgtaggatt tggtacaggt
    31321 aaagctcaag aagttcctga agcaatccgt aaagcaattg aagatgcgaa gaaaaactta
    31381 gttgaagtac ctatggttgg ttcaacaatc ccacacgaag taatcggtgc ttttagtggt
    31441 ggtagaatcc taatgaaacc tgctgtagaa ggttctgggg ttgctgctgg tggaccagtt
    31501 cgtgccgtat tagaattagc tggggtagca gatatcactt ctaaatcttt aggctcaaat
    31561 acaccaatca acgttgttcg cgcaacagtt gaaggtttga aacaattgaa acgtgcagaa
    31621 gaagttgcag aacttcgcgg caaatcagtt gaagaattaa tcggataagg aggacaacaa
    31681 taatggctga attaaaagtt actttaaaac gcagtattat cggacgtcct caaaaccaac
    31741 gcgatacagt taaagcgtta ggtcttacaa agattaacag ttcagtggtt aagccttcta
    31801 atgaagcaat taaaggcatg atcaatactg tttctcacct agtggacgtt gaagaaattt
    31861 aatttagaaa aatcataact agaacattaa ggaggtgcca aacctatgaa acttcatgaa
    31921 ttaaaacctg ctgaaggttc acgccaagta cgtaaccgtg ttggtcgtgg tacttcatct
    31981 ggtaacggaa aaactgccgg acgcggacaa aaaggacaaa aagctcgttc aggcggcggt
    32041 gtgcgtttag gatttgaagg gggacaaaca ccattgttcc gtcgtttacc aaaacgagga
    32101 ttcacaaacg tgaaccgtaa agactatgca gtcattaatc ttgatgtatt gaaccgcttt
    32161 gaagacggag ctgaagtaac tcctgttgct ttagttgaag ctggaatcgt gaaaaacgaa
    32221 aaagctggaa tcaaagtatt agctaatggg gaactaaaca agaaattaac tgtgaaagca
    32281 gcaaaattct ctaaagcagc tcaagaagca attgaagcgg ctggtggatc aatcgaggtg
    32341 atctaatgtt tagactatta aaagatgctt ttaaagtaaa agacattaga tcgaaaatcc
    32401 tatttactgt atttgtgttg tttgtatttc gcctaggtgc acatattact gtaccaggcg
    32461 taaatgctgc ttcattgcaa aacctttctt ctttgccatt tttcaacatg ttgaacttgg
    32521 taagtggtag tgcgatgcaa aacttttcca ttttctcaat gggggtttca ccatacatta
    32581 ctgcttctat cgtcattcag cttcttcaaa tggacatcgt gcctcgattt gttgaatggt
    32641 cgaaacaagg ggaagttggt cgtaaaaaac tgaatcaagc gactcgatat ctgacattag
    32701 tattggcatt tatccaatct gtcggtatca ctgctggatt caatcaatac gctcaattag
    32761 gtttcgtgaa taatcctaac gtagcaactt acgtaacgat cggtttgatt ctaacagccg
    32821 gtacgatgtt tgttacttgg ctaggagaac agatcacaga aaaaggtatc ggaaatggcg
    32881 tatcaatgat catttttgca ggtatcattt ctagattgcc tgaaagtatc agagaaattt
    32941 atgaagatta ctttataaat attgatcctt cagacatatg gaaatctgct atttttgtgg
    33001 ccattttact agtagcggtc ttgttgatcg tgacattcgt aacatttttc caacaagctg
    33061 aacgcaagat cccgatccaa tatacgaaac gcgtagctgg cgcgcctaca agcagctatc
    33121 tgccgttgaa agtaaatgca gctggagtta ttccagttat ctttgctagt tcgtttattg
    33181 ctacgccaaa tgctgtttta caagcattga gcggtagatt cggttctgaa aactggtaca
    33241 aagtgatgat ggagatcttt aactataata cagcacctgg tgctacgatt tatacgattc
    33301 tgatcgttgc cttcactttc ttctacgcct ttgttcaagt aaaccctgag aaattagcgg
    33361 aaaacttgca aaagcaagga agttatatac caagcgtacg ccctggtaag gggactgaag
    33421 agtatgtctc aagattactg atgcgtttaa gtacagttgg tgcagtcttc cttggtttag
    33481 tggcattgct tccaatcatt gctcaaatgg tttgggatct tccacaatca atcggattag
    33541 gcggtacaag cttactgatc gttatcggtg tagctttaga aactgcgaaa cagttagaag
    33601 gcttaatgct gaaacgtaaa tatgttggat tcattgagta agaaagcgtg ttgaggtttc
    33661 ccctcaacac acgttctcac acatttatta ggagggaaaa caatgaacct cattttaatg
    33721 gggctgccag gcgcaggtaa aggaacacaa gctgaaaaaa tcattgatgc ttatggtatt
    33781 ccgcacatct caacaggaga tatgttccgc gcagcgatgc aaaacgaaac agctcttggc
    33841 ctggaagcaa agtcttatat ggataaaggt gcattagttc ctgatgaagt aacaaatgga
    33901 atcgtgaaag agcgactagc tgaaccagat acagagaaag gcttcttatt agatggcttt
    33961 ccacgtacgc tagatcaagc aaaagctctg gacgcaatgc taaaagattt gaataaaaaa
    34021 attgatgctg tcatcgatat ccatgttggt gaagaaatct tggtcgaacg tctagccggc
    34081 cgatttatct gcagcaattg tggagcaact taccataaaa ttttcaaccc tacaaaagtt
    34141 gaagacacat gtgatcgttg cggtgggcat gaattctatc aaagagaaga tgataaacct
    34201 gagacggtta aaaatcgtct ggctatcaac atcaaaaata gtgaaccaat cttggcttat
    34261 tataaagaac aaggtttgct aaacactatt gatggtgctc gtgaaatcga tgctgtattt
    34321 gcagatgtac aaaaaatcat tgaaaaatag cacgattgcc cgtagccgac ttgcattatg
    34381 cagttactct tgtcaagttc aataaattat gatagaatat gtcagtccga cttctagaga
    34441 atcctctaga tgatccattt tgagaatttt gaggtgggaa ctgcagtttc cgccatttta
    34501 tcgtgtgaaa atgcgaaaca agtacaagga ggtactgtgc gtggcaaaag aagatatgat
    34561 tgaagtcgaa ggtacagtcg tcgaaacttt gccgaatgca atgtttaaag ttgaactaga
    34621 aaacggacac caagttcttg ctactgtttc aggtaaaatt cgtatgcatt acatccgtat
    34681 cttacctggt gacaaagtaa cggttgaatt gtctccatac gatcttaacc gtggccgtat
    34741 tacttatcgc tttaaataat tgtactccgt aaaactacag gaggtattat catgaaagta
    34801 agaccatcag taaaaccaat gtgcgaacat tgtaaagtaa ttcgtcgtaa aggacgcgtt
    34861 atggtgattt gcccagcaaa tccaaaacat aaacaacgtc aaggataatt ggaggtgcta
    34921 taaatatggc tcgtattgca ggagtagata ttcctcgtga taaacgtgta gtagtttcac
    34981 ttacttatat ctatggtatt ggtaacacta ctgctaaaaa gatcttagct gatgctggtg
    35041 tatctgaaga tgttcgcgtt cgtgaattaa cgaatgaaca aacagatgct atccgtgcag
    35101 aagtagataa attaaaagtt gaaggggatc ttcgtcgtga agtgaacttg aacatcaaac
    35161 gtttgatgga aatcggttct taccgtggaa tccgtcatcg ccgtggattg cctgttcgtg
    35221 gacaaaacac gaaaaacaat gcacgtactc gtaaaggccc agctcgcgct atcgctggca
    35281 agaaaaaata attcttaagt gaaggaggtt aaaacttcat ggcagcaaaa aaagtgaatc
    35341 gtaaacgccg tgtgaagaag aatatagaag caggtattgc acatatccat tctacattca
    35401 ataacactat catcatgatc actgatactc acggaaatgc attagcgtgg tcatcagctg
    35461 gttcattagg tttcaaagga agcaaaaaat caactccttt tgccgctcaa atggcagcag
    35521 aaactgctac aaaagcagca atggaacacg gattaaagac tgttgaagta acagttaaag
    35581 gacctggttc tggacgtgaa gcagcaattc gttcattaca agctacaggt ttagaagtga
    35641 ctgcaattcg tgacgtgact ccagttcctc ataatggtgc ccgccctcca aaacgccgtc
    35701 gtgtttaatg agtgccgctc attttgagtt ttatcagtaa cgtcattgaa caagacactt
    35761 ttcgttttga aaggggtaaa gataagaatg attgagttcg aaaaaccaag aatcgcaaaa
    35821 attgatgaag aaaaagatta tggcaagttc atcgttgaac ctcttgaaag agggtatggg
    35881 actactttag ggaattccct acgtcgtatt ttattatctt ctttgccagg tgctgccatc
    35941 actaatattc aaatcgatgg tgtactgcat gaattctcta ccatcaaagg tgttagagaa
    36001 gacgtcactc aaatcatctt gaacattaaa ggcttagcgc tgaaaatgta tggccaagaa
    36061 gaaaaaaccc ttgaaatcga tattaccgga cctgctacag taactgctgg cgatattatc
    36121 gttgatagtg atgtagaaat tcttaacaaa gatatgtaca tctgtagtgt ctctgaagga
    36181 gctacgttcc atgctcgctt aacagtgaaa cctggacgtg gttatgttca agcagatgaa
    36241 aataaaaaag aagatatgcc aattggcgtt cttccagtcg attcaatcta cacacctgtt
    36301 cgtcgtgtga actatcaagt tgagaacaca cgtgttggac accgtgaaga ttttgataaa
    36361 ttgacgatgg agatttggac agatggttca attgaaccgt tggaagcaat gagtttgtct
    36421 gcgaaaatca tgactgaaca tttggatatt tttgtgaact taactgatga agcaaaaaat
    36481 gctgaaatca tggtcgaaaa agaagaaaca caaaaagaaa aaatgttgga aatgacgatc
    36541 gaagagttag acttgtctgt tcgctcttac aattgtttga aacgtgcagg aatcaatact
    36601 gtacaagaac ttacaaataa atctgagcct gaaatgatca aagtccgtaa cttaggacgt
    36661 aaatcattag aagaagttaa agcaaaattg cacgacttag gtttaggttt acgtaaagaa
    36721 gattaaaacc ttttacgaag gagggaaacc aacgtgagtt atcgtaaact aggacgcaca
    36781 tctagccaac gtaaagcgat gttgcgtgat ttaacaactg atttattaat caacgaacgt
    36841 attgtgacta ctgaagctcg tgcgaaagaa gttcgctcaa ctgcagaaaa aatgatcact
    36901 ttaggtaaac gcggagattt gcatgcacgt cgtcaagctg cggctttcgt acgtaatgaa
    36961 gttgccagcg tgcgtgaaga aaatgaggac atcgtcattg aatcagcttt acaaaaactg
    37021 ttcaacgata ttgcacctcg ttacgcagaa cgtcaaggcg gatacacacg cattttgaag
    37081 acagaaccaa gacgcggtga tggtgcacca atggttgtct tagaacttgt ctaaaatcac
    37141 actaataaca tcactttata gatgattctt ttagagtgtt atgatgttgg aagatatttt
    37201 tccgagtcta gctcagcgct gctcctcaag cgaatatcgc ttggggagta agcactcatc
    37261 ataaagtgtt tttttatacc ttcaattacc tgtcatgaca gatatgtttg aaggcataaa
    37321 aaaacatttt aagcgagaaa taattgaaaa ttttgtcttt ttttgatagt gttagtagga
    37381 actttaggat gaagaaggag taatcatgga gccaattatt gaattagaaa agataaatta
    37441 taaataccag ccagatgatc ttcgccccgc attgaaagat gtctccttta caattgataa
    37501 aggtgagtgg atcgctatta ttggacacaa tggttcagga aaatcgacac ttgccaaaac
    37561 gatcaatgga ttgcttctgc cagaatcagg gatcgtgaaa gtgggaaatc aaatattgga
    37621 tgaagaaaat atttggacga tccgacagat ggttggaatg gtttttcaaa atccagacaa
    37681 tcagtttgtt ggttcgacag tagaagatga cgttgctttt ggtcttgaaa atcaagggat
    37741 tcccagagaa gaaatgctag tgcgtgtgaa agatgcactt gagaaagtta gaatggctga
    37801 gtttgcttca cgcgagcctg ctcgtttatc aggagggcaa aaacaacgtg ttgccattgc
    37861 cggcgtggtt gccttacgac ctgatatcat tattttagat gaagcaacca gtatgctaga
    37921 tccagaaggt cgtgaagaag tgatctctac gatcaagaag atcaaagaag aaagtcaatt
    37981 gaccgttata tctatcactc atgatattga tgaagcagct aatgccaatc ggatactagt
    38041 gatgagacaa ggcgaacttg tccgtgaagg tacaccaaaa gaaatttttt ctgctggtcc
    38101 tgaactgatc gacttaggtc tagatctacc atttcctgaa aaactgaaga gcgcactgaa
    38161 agaacgtggg gttgatgtgc ccagtgaata tatgacagaa gaaaggatgg tggattggtt
    38221 atggacatcc gttttgaaca agtagacttc acttatcagc cgaatacacc atttgaacaa
    38281 agagccttat ttgacatcaa tatgacgatc aaagaaaaca gttatactgc attagtaggg
    38341 cataccggaa gcgggaaatc aaccttactt cagcatttga atgcgcttgt taagccaaca
    38401 agcggtacag tccatattgg agaacgagat attcagccgg atacggataa taagaattta
    38461 aagcccatcc gaaaaaaagt tggcatcgtc tttcagtttc cagaagcaca gctattcgaa
    38521 gaaacggtag caaaagacat tgctttcggt cctaaaaact ttggtgtcag cgaagaagaa
    38581 gcactagtcc tagcaaaaga aacattagaa caagttgggc tggatgaaag ctatttggaa
    38641 cgttcgccat ttgaactttc aggagggcaa atgcgtagag tagcaatcgc tggtgtgctt
    38701 gccatgaggc cggaagtact tgtattggat gaaccaacgg caggacttga tccccagggc
    38761 agaaaagaaa tgatggagat gttttggcgt cttcataagg aacagcagat cactattgta
    38821 ttagttacgc atttaatgga tgatgtagcc aattttgcag attatgtcta tgtgttggaa
    38881 aagggacgaa tcgttaatag cggggagccg caagaggtct ttcaaaacat tgaatggcta
    38941 aaagaaaaac aactaggcgt acctactgcc accgagtttg cagaagaatt aatggctaaa
    39001 gggatgaact ttgctacctt gcctttgaca gctgaagaat tagcagacgc tatcgtccat
    39061 tatgctggag gaaatgccca tgatgaataa attgatattt ggtcgttata tgcctggaga
    39121 ttccttgatc catcgattgg atccccgagc taaattatta gccagttttt attttatcgg
    39181 aatcattttt ttagcaaata attgggaaac gtaccttttg ttggcatttt tcacattact
    39241 ttctgtcttc ttgtcaaaag tagatttgaa attttttatc cgaggcattc gcccattgat
    39301 ctggctaatc ctcttcactg tagctttgca ggtcttgttt acaagtggcg gagaaactta
    39361 ttggagttgg ggtgttttcc atatcactga atttggtgtg atgaacggcc tctttatttt
    39421 ctgtcgattc gtgttgatca tttttatgtc gactctactc acgcttacta caccgccttt
    39481 ggaattatcg gatgcgattg agtacttgct tcgtccattg aaagccgtac ggttcccagt
    39541 ccacgaaatt tcattgatgc tgtcgattgc tcttcgtttt gtgcctacat tgatggacga
    39601 aacagaaaag atcatgaacg ctcagcgcgc tcgcggtgtt gattttggtg aaggaaacct
    39661 agttcagaaa atgaaagcag tcattccttt gctgattcct ttatttgtca gcagcttcaa
    39721 ccgtgcggaa gatcttgcga ctgctatgga agcaagaggc tacaaaggtg gggaaggaag
    39781 aacgaaatat cgtgtattgc attggcataa tagagacacg atggttatca ttgcttttgt
    39841 attattaact gtgctactga tctttttaag aggataaacg ttcagtgggc cgctatctat
    39901 tcttttcttg ctgatctgtt tgcaagaaag gatgcaagca gtgtatgaca agaaatatta
    39961 gggtctgtgg cagactaagg tcacaggcct ctttctttta tctgaaaaga aagaacgaaa
    40021 aaattttata gaaaaatcat tattccagat aaccttttag gaacaaagga gagaaaaaca
    40081 tggtacgtta caaagcaatc attgcgtacg acggaacaaa ttttaatgga ttccaaaaac
    40141 agccaaatgg gcgtactgtt caagaagaag tagaaaaaac attacaaaaa atggcaaatg
    40201 gaaaagaaat cacggtcttt ggttcgggaa ggacagatgc cggagtacat gcgatcgggc
    40261 aagtaatcca ttttgattat ccagaggagc gtccgttaga acgaatgcgt ttcgcgctag
    40321 atacccagtc accagaggat attgctgtaa gacaagtaga aattgtttca gaagacttcc
    40381 acgcaagata tttggtcaaa gaaaagacgt atcaatttcg agtggatata gggaaaccac
    40441 gcagtccttt tcgacgccat tatgcaagtt attttcctta cccacttgat ttggaaaaaa
    40501 ttcagcgggc gcttcctgat ttattaggga ctcacgattt cacctctttt tgtgcatcag
    40561 gaagctcgat agaggataaa atacgtacga tctacgaggc taagatggag gtaaatgaaa
    40621 caaaagacga attgttgttt acatttaggg gaaatggctt cttgtataag atgatacgga
    40681 tcttagtagg aacgcttttg aaaattggca atggccgatt gcctgaagat agtatcccag
    40741 atattattgc gaaaaaagat cgtaatgcag caggacctac agcacaccct gaaggacttt
    40801 atttgtatga agtgaaatat gaataagagg ttatgataga agtggtcagc ttcaagaaat
    40861 aagaaggtat ttacgaaaat tacttctcaa aattttgcaa aatttcggct tattttcgaa
    40921 gaagctactt ctggaacacc gtttaaggaa gaggtcgtga aatcagcgtt ttgacctgag
    40981 tattagagaa acaaacggga aaatagctcc tcatattttg ccgtttgttg agctaatacc
    41041 gaaggtcagc tgattgaaca ccgtttaaga aagaggtcgt gagcctgtca tttaactctg
    41101 agtcacaaag agcaatttca aaaaacagct tctcatcttt gatgaatatc gtgacttgtg
    41161 gacgaggcgt tgataagtga atcatctttt tgttacctat tttttctgta accaaaaaac
    41221 gatggaacgt tgttttgatt gcccttggtg cggacagtct gaacaggttc catcgtttct
    41281 ttattttcta cgtcttcttc tgtgtgtgtt tcgtggttgt tcttctattg ctggatcaat
    41341 cgtgccataa aaggggcgat tgcggtcaag gtaccagaca taaagaataa acaatgcaat
    41401 acataggaca aaaaggattg cacctatcgt aaatccttca ggcaagtaac tgaaagtgac
    41461 agtatgttta cctgctggaa cttttatcat caagaatgcg tctttgaaat tgctgacctt
    41521 gactggtttc ccatcaattt tagctttcca tcctttgtca taaggaatag ttgtcaccaa
    41581 catggaatct tcagcggtat ttacagtccc agaagctttt ctgccagtgg cttccatttc
    41641 gacacctttt tctttgatct gattcattgc ggcttgatag gctacaacat ctagaccaac
    41701 gactttaggc tccatgaaac ttatttcttt cgtcccataa aaacttgcag taaatgtcac
    41761 tgttgtctct tgaggataat agccaagatc atagtattga ccagaaatgt tgatttgcgt
    41821 ttttcgcgat gtaccattga cagtgatcgt cgcactagaa ctttctaatt gcgcaaaatt
    41881 ggttgggaac aaacttaagt aagcttgtgt atttgcaggc acatggactt cccaagtgag
    41941 ctcttttggc tggttatctg ctgtttctga gtaagtgacg ccagtcgttg tctttgtgat
    42001 cgtcgtattc aactgattct tcaaagtagg tgtatagaat gtaaaatact gttcatttag
    42061 accagacaat gtattgatca agtttgtctg actggtcaaa ttatctgtca ctggctgctc
    42121 aacatcatta acggacaacg gtgcagtaaa tgctagtggc aatgcattga cattctgata
    42181 cagtgtatag tcgccgcttt gaccagcttg gcggaagcca tatttactga acgtgctttc
    42241 gtttttagaa atgttatatt tgattcctgt aaacccatcc atcagcaaag tattattgtt
    42301 ataacggata ttcaaatttg tccctcttga acggaaacct aatttatcca ggtaagagga
    42361 agaattacga tttcttatag aagaaaacaa actgatacca ctgtaaccat agttgatcga
    42421 atcgtttgca gatactggat ccagattttc caagcggtaa aacgagtcgg aatctttttt
    42481 tgtttcattt acaagagctt tcaagtcagg ataaggatca gtatataaac tgcgggaagc
    42541 ataattccaa tcatcaagaa tgccatggat catactgttc gtattgacca gtgcttcagc
    42601 agacatgatc aataataaca atatactcag atgattgaat gaaagttttt tatgctgata
    42661 aaatcccaca gcaaaagcat aaatccctaa gaaaaccact gtcaatacaa aggaagtcat
    42721 cgtcacatag gtgtagcttc cttcaggttt gatgccccat gccaccgcaa agagagcagc
    42781 tacgaccaaa atgattccta acaacagccc acgatcgtct tgttctatct tttcccatcc
    42841 ttttcctgca agcaggagca ctaagaaaga gaacagatag gcataacgga ataaaaacat
    42901 attcggtgca tgcataccat gccagaacaa attcaatgga acaagataaa aacttgcgat
    42961 aagtatggcg aaaagactgc cgtacaaaaa tttatttttc aacggtactt tccgactggc
    43021 aaaataaaac agacagagga ctaatggtag cagacctgcg taaacgaacg gaatcgatcc
    43081 gtattttgtc gtgtcataaa caccaatcat atttttcatg attagatcca gataagaagt
    43141 cgcttctgtt ttcaacgtag tgatctgact cagtgcctcg ccatttgacc gaagatccag
    43201 cactgctggc aatacaatga tcattgaagc gccgcctgcc agtaaagaag tgataccata
    43261 aggaacaatc gtttttttat attctgacca gtttgtcaat agacgggcga taaaatataa
    43321 gaaagaaaat acgccaatca taaaacccat ataaaaactg gataaaaaca agagtaaata
    43381 actgataaat aaaagtttcg gccgtttaag atccataaca cgatgaatcc ccaaaatcac
    43441 taatggaaga taaaaaaatg catccagcca catgatgatc tccgaatgtg cagtgataaa
    43501 tgagatcaat gcgtaactga cgctaagtgc aacatgcccc cagcgaggaa tcttgaacgt
    43561 acgtgcaaga aaccaaaaag ccaatccggc acagccgacc ttaagcaaag tcagaacata
    43621 cagtgcatct ggcatcatct ggtttggaaa aaagaacacc agtggcgtga agatcccgcc
    43681 tagatagtaa gaaattaaag ataaataatt caatcctaga gaagcattcc atgtataaaa
    43741 aatactttgc tttccaagaa gaacatttcg gaagctggca tgaaaattag aaaactgtga
    43801 aaaggcatca cttgccaaaa tacttctgct gcttccagga tagattccga tacttagata
    43861 aacaagtgct aaaatcaaaa acggaatcag gaaactagct gctgcgtagc gtccgttttc
    43921 tctaaagaat gactttaaat tttttttcat atttattttc caccaaattt ctatgaagtt
    43981 gtattcagtt tatcatatcc ttgtttttct ctgcttaaaa agagtctagt tttcacaaaa
    44041 aaataaaatt tagaataaat aactt
//
LOCUS       DACECZ010000008        43590 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.008, whole genome shotgun sequence.
ACCESSION   DACECZ010000008 DACECZ010000000
VERSION     DACECZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.008"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            160..840
                     /locus_tag="IWO35_000495"
     CDS             160..840
                     /locus_tag="IWO35_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0315576.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            930..1964
                     /locus_tag="IWO35_000496"
     CDS             930..1964
                     /locus_tag="IWO35_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0315577.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1978..2313
                     /locus_tag="IWO35_000497"
     CDS             1978..2313
                     /locus_tag="IWO35_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315578.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2297..4777
                     /locus_tag="IWO35_000498"
     CDS             2297..4777
                     /locus_tag="IWO35_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315579.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4833..6392
                     /locus_tag="IWO35_000499"
     CDS             4833..6392
                     /locus_tag="IWO35_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0315580.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6389..7753
                     /locus_tag="IWO35_000500"
     CDS             6389..7753
                     /locus_tag="IWO35_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0315581.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7924..8994
                     /locus_tag="IWO35_000501"
     CDS             7924..8994
                     /locus_tag="IWO35_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0315582.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9009..9677
                     /locus_tag="IWO35_000502"
     CDS             9009..9677
                     /locus_tag="IWO35_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0315583.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9697..10275
                     /locus_tag="IWO35_000503"
     CDS             9697..10275
                     /locus_tag="IWO35_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0315584.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10676..11587
                     /locus_tag="IWO35_000504"
     CDS             10676..11587
                     /locus_tag="IWO35_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0315585.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11965..12882)
                     /locus_tag="IWO35_000505"
     CDS             complement(11965..12882)
                     /locus_tag="IWO35_000505"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ0315586.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13084..13521
                     /gene="zapA"
                     /locus_tag="IWO35_000506"
     CDS             13084..13521
                     /gene="zapA"
                     /locus_tag="IWO35_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ0315587.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13579..14127
                     /locus_tag="IWO35_000507"
     CDS             13579..14127
                     /locus_tag="IWO35_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ0315588.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14242..16602
                     /locus_tag="IWO35_000508"
     CDS             14242..16602
                     /locus_tag="IWO35_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0315589.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16689..17003
                     /gene="trxA"
                     /locus_tag="IWO35_000509"
     CDS             16689..17003
                     /gene="trxA"
                     /locus_tag="IWO35_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ0315590.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17185..18963
                     /gene="uvrC"
                     /locus_tag="IWO35_000510"
     CDS             17185..18963
                     /gene="uvrC"
                     /locus_tag="IWO35_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ0315591.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19108..20007
                     /locus_tag="IWO35_000511"
     CDS             19108..20007
                     /locus_tag="IWO35_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0315592.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            20000..21262
                     /locus_tag="IWO35_000512"
     CDS             20000..21262
                     /locus_tag="IWO35_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0315593.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21423..23321
                     /locus_tag="IWO35_000513"
     CDS             21423..23321
                     /locus_tag="IWO35_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ0315594.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23651..24370)
                     /locus_tag="IWO35_000514"
     CDS             complement(23651..24370)
                     /locus_tag="IWO35_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ0315595.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            24707..25039
                     /locus_tag="IWO35_000515"
     CDS             24707..25039
                     /locus_tag="IWO35_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315596.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25026..27029
                     /locus_tag="IWO35_000516"
     CDS             25026..27029
                     /locus_tag="IWO35_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ0315597.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27048..27518
                     /locus_tag="IWO35_000517"
     CDS             27048..27518
                     /locus_tag="IWO35_000517"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ0315598.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27539..28123
                     /locus_tag="IWO35_000518"
     CDS             27539..28123
                     /locus_tag="IWO35_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ0315599.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28138..29124
                     /locus_tag="IWO35_000519"
     CDS             28138..29124
                     /locus_tag="IWO35_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ0315600.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29114..29425
                     /locus_tag="IWO35_000520"
     CDS             29114..29425
                     /locus_tag="IWO35_000520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ0315601.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29447..31228
                     /locus_tag="IWO35_000521"
     CDS             29447..31228
                     /locus_tag="IWO35_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ0315602.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31221..32597
                     /locus_tag="IWO35_000522"
     CDS             31221..32597
                     /locus_tag="IWO35_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ0315603.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32604..33239
                     /locus_tag="IWO35_000523"
     CDS             32604..33239
                     /locus_tag="IWO35_000523"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ0315604.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33269..34624
                     /locus_tag="IWO35_000524"
     CDS             33269..34624
                     /locus_tag="IWO35_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ0315605.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34816..36210)
                     /locus_tag="IWO35_000525"
     CDS             complement(34816..36210)
                     /locus_tag="IWO35_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0315606.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRSNQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36302..37273
                     /locus_tag="IWO35_000526"
     CDS             36302..37273
                     /locus_tag="IWO35_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ0315607.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37270..38052
                     /locus_tag="IWO35_000527"
     CDS             37270..38052
                     /locus_tag="IWO35_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ0315608.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38221..38592)
                     /locus_tag="IWO35_000528"
     CDS             complement(38221..38592)
                     /locus_tag="IWO35_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0315609.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38726..39169
                     /locus_tag="IWO35_000529"
     CDS             38726..39169
                     /locus_tag="IWO35_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0315610.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="IWO35_000530"
     CDS             complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="IWO35_000530"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ0315611.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(40040..41407)
                     /locus_tag="IWO35_000531"
     CDS             complement(40040..41407)
                     /locus_tag="IWO35_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0315612.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41391..42221)
                     /locus_tag="IWO35_000532"
     CDS             complement(41391..42221)
                     /locus_tag="IWO35_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ0315613.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42384..42821
                     /locus_tag="IWO35_000533"
     CDS             42384..42821
                     /locus_tag="IWO35_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ0315614.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42897..43517
                     /locus_tag="IWO35_000534"
     CDS             42897..43517
                     /locus_tag="IWO35_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ0315615.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        14279 a         7487 c         9251 g        12573 t
ORIGIN      
        1 ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct cttttataca
       61 aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc atactgtctc
      121 tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa catgattggg
      181 acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt tgctgagggt
      241 caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt ggagcctctt
      301 aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc agcaactggc
      361 cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac acacgagatt
      421 gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc ttatctccaa
      481 gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa cgtgacagtt
      541 tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt agattttgga
      601 acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag tcaaactgta
      661 actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa agatcaaggt
      721 ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt gccgaaagca
      781 gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc agtagcatag
      841 agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga ttttttaccg
      901 cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg tttgtttcgt
      961 ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca aaggattcag
     1021 caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa gcaatcggtt
     1081 attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt atcagcaatt
     1141 cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg agtgatggtg
     1201 gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg tttgatgtcc
     1261 gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca gaaacaactg
     1321 tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta gcgggtattc
     1381 atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca caaatcaaaa
     1441 atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg atagaaccgg
     1501 atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact gttcagcttc
     1561 gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc aagatctttt
     1621 atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca ccaaattcga
     1681 attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat taccgcgcag
     1741 aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac tttacgatca
     1801 aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa gaacaatctt
     1861 tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg attttgctgt
     1921 ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt gatttcgttg
     1981 aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag ctggcaagag
     2041 ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct aagtccaatt
     2101 ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct accgatttat
     2161 aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat ccgttttctt
     2221 tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg gagaagaaag
     2281 gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct ccttcttttt
     2341 tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag ctgttggagc
     2401 aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac ctccaaatgg
     2461 tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta accaattgcc
     2521 taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag gtggcacggt
     2581 ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg tgaatatcac
     2641 tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat caagcagtga
     2701 tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg cccccatcaa
     2761 ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg ggatggggtc
     2821 gtgggataca aatactctat tgacagatat tattgctatt attacgcgag gcgaattcaa
     2881 cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga tgacagaaaa
     2941 cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat tacaaccttc
     3001 tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata ttatccaaga
     3061 tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta aattaactgg
     3121 cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa atacgtggaa
     3181 tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg ataccacaga
     3241 atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga cattgacgat
     3301 ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc gcgtagtcaa
     3361 ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc cagcaagcac
     3421 aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc aaggcggatt
     3481 gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa cggttttacc
     3541 aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat cgtaccaagt
     3601 cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca ggctttattc
     3661 gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa cgatccaatc
     3721 aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt cagggcaata
     3781 ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt cttttgcgta
     3841 caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag tggctaattt
     3901 tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg agacaggaga
     3961 tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca cgttaacaaa
     4021 atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa atactactta
     4081 tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca ttcaacaagg
     4141 agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc ttacttacaa
     4201 ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga caaaggcagc
     4261 tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc cagttgtcca
     4321 aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta atgtaccaga
     4381 tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt atgaatcaaa
     4441 aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc cttggcaact
     4501 gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa gcggaacaaa
     4561 attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa acacgttgat
     4621 ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg ataccgatgg
     4681 tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt atcaaggcat
     4741 ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt gtatggatct
     4801 gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg ctgttaaaat
     4861 cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa aatgaacaac
     4921 ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc gttaataacg
     4981 tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt tatttacaag
     5041 ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat attttattaa
     5101 tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg cgctattcta
     5161 caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg cagaaacttg
     5221 tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa ttaaatgatt
     5281 ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca gaaagtcaaa
     5341 ttcgttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa aacaaaaaag
     5401 aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag tttagttttg
     5461 ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg aagaagcgaa
     5521 tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga ttgtttcaaa
     5581 cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt caatatgcct
     5641 ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt aactttacat
     5701 tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac aaagacgacg
     5761 tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc tctttttatg
     5821 tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat cacgctcatt
     5881 ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa cagcggttaa
     5941 ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga caaacatttg
     6001 ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta tttgctcgct
     6061 atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa attgcttatg
     6121 atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag gaacaattgc
     6181 tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat gatatcattt
     6241 tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt gtattcacta
     6301 gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa tgttatttag
     6361 aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg cttcccatga
     6421 aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag ttttactgat
     6481 cgatgaagat cataaagatg gactaggcta tgtttcaaat ggaatcaacc tttatttaaa
     6541 aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc aagaaagtgg
     6601 ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac atcagctcat
     6661 cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa tcgtagcgac
     6721 aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct acacctataa
     6781 aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag aagtagtgat
     6841 cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata aaggatacat
     6901 tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata aggatatgat
     6961 caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa atgaattctt
     7021 gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt ctcaaacaat
     7081 aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat tgttaaaaga
     7141 aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc agacaagtga
     7201 tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa ataaacggca
     7261 tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag cgctaacttt
     7321 gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag tcattataga
     7381 ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg aacaaaatac
     7441 cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa acaaaacagt
     7501 aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac agctgataag
     7561 ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac acaaaactac
     7621 gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct ctcgttttca
     7681 ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca atagagtaga
     7741 taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata aatattaatc
     7801 aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag cataatattc
     7861 gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag gaggaggagt
     7921 taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc gttagttgca
     7981 ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc ttatggctta
     8041 gctcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt gctttcgaaa
     8101 gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga agaagtcgaa
     8161 ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa agacagtaca
     8221 gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt catcatgccc
     8281 aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga tattagtttg
     8341 aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag tgacaagacg
     8401 tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt tctctataca
     8461 accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga aaatgcacgg
     8521 attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt agaacagatc
     8581 aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt gacttttgga
     8641 tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt catttatgta
     8701 ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat cacaggagga
     8761 tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg tattcttctt
     8821 ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc attccaaagc
     8881 aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt gttaggcgca
     8941 ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg ataagaagga
     9001 ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa gatggcgacc
     9061 aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa tttgttgcaa
     9121 tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc ggattgcaga
     9181 cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa aaagaaagcg
     9241 aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc aatctggttc
     9301 cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat gaacatcgtc
     9361 aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc aacaagtatc
     9421 cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg
     9481 atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa aaggcacgag
     9541 aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg atcatggtta
     9601 ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa gatggtgtat
     9661 tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata caacaaagat
     9721 acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg atatctcggc
     9781 acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct ctatcattat
     9841 ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa agatgttgga
     9901 aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa
     9961 atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct tcacgatctt
    10021 gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa gaaaaattat
    10081 cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa tgggatttca
    10141 aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac tcggtcgaaa
    10201 agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt gaatggtgta
    10261 ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga agcaagatat
    10321 gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat acaaatttgt
    10381 tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc caggaagtgt
    10441 tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat ttattgggta
    10501 gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc
    10561 attttagcag taatgttatt ttctacggtt atatctttta cagccccttt tttgcttact
    10621 taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt caagaatgaa
    10681 aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt ttcaaataga
    10741 tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca gtacggttac
    10801 aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa
    10861 tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta taaacggcgg
    10921 agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga caaatgatgg
    10981 ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa
    11041 accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg gaaatgatac
    11101 tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa gtacagccat
    11161 aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc atactacaga
    11221 tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt
    11281 tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa tcacgacata
    11341 ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct tcactgatct
    11401 gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag aaaatcttca
    11461 accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa aggaattagt
    11521 aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga ccacttattt
    11581 tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg tttggccggg
    11641 cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt gagcctgccg
    11701 ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt gatgactatt
    11761 gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt tgtgaaatca
    11821 acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    11881 tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac aagggactgt
    11941 gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt tttctgtatt
    12001 ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt
    12061 tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc cagcttcagc
    12121 tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac
    12181 agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt
    12241 ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc
    12301 ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc
    12361 gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa
    12421 gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct
    12481 atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg
    12541 tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc
    12601 actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg
    12661 cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc
    12721 ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc
    12781 aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg
    12841 actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt
    12901 atacataaaa ctataccgca actcacgact tttcgttttc taagagattg cgagacatag
    12961 cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg
    13021 tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt
    13081 cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt
    13141 atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta
    13201 gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct
    13261 gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct ggaagaagaa
    13321 acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc
    13381 attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat
    13441 accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt
    13501 aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaagttag
    13561 aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt
    13621 ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg
    13681 tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt
    13741 ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt atttgccttt
    13801 cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg
    13861 ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga
    13921 caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt
    13981 ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag
    14041 catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag
    14101 ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg
    14161 tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat
    14221 agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa
    14281 gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa
    14341 ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac tgaggatgga
    14401 ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa tatccgtccc
    14461 catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc
    14521 agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc tgacagtgaa
    14581 atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc
    14641 cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag
    14701 ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat
    14761 gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat
    14821 gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac
    14881 gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt ggatttgaat
    14941 aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa
    15001 ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta tgtgattggt
    15061 aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc catcgtgccg
    15121 ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag
    15181 gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca
    15241 ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg
    15301 gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa
    15361 gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct
    15421 catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt
    15481 gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta
    15541 gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa
    15601 gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca
    15661 ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc
    15721 tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat
    15781 gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag
    15841 accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg
    15901 aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa
    15961 aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt
    16021 cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca
    16081 aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag
    16141 aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc
    16201 caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta
    16261 aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa
    16321 cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt
    16381 cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt
    16441 ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag
    16501 ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca
    16561 aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc
    16621 agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg
    16681 tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac
    16741 ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt
    16801 tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg
    16861 atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga
    16921 aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc
    16981 aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa
    17041 tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt
    17101 gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta
    17161 attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt
    17221 cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc
    17281 aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa
    17341 acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc
    17401 gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg
    17461 ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg
    17521 gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc
    17581 ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa
    17641 cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct gtgtccttat
    17701 tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta
    17761 aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa
    17821 aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc
    17881 atgactcgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg
    17941 attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa
    18001 cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc
    18061 ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg
    18121 atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga
    18181 gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg
    18241 tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac
    18301 gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg
    18361 acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat
    18421 cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc
    18481 atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg
    18541 atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga
    18601 atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa tgagttgcta
    18661 ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc
    18721 cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt
    18781 aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa
    18841 atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta
    18901 caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa
    18961 tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa
    19021 gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat
    19081 gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt
    19141 gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg
    19201 ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg
    19261 acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat
    19321 gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa
    19381 ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac
    19441 ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct
    19501 aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc
    19561 attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga
    19621 atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat
    19681 gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga
    19741 aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta
    19801 agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat
    19861 acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact
    19921 cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa
    19981 ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa
    20041 aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat
    20101 catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt
    20161 ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt
    20221 caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc
    20281 gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc
    20341 attaggacaa agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct
    20401 tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag
    20461 tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa
    20521 ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt
    20581 gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga
    20641 agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt
    20701 acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca
    20761 attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat
    20821 tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt
    20881 tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt
    20941 gccagtgact tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga
    21001 tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat
    21061 gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt
    21121 acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt
    21181 actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag
    21241 gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt
    21301 gttcccagct tctttttgta tagacctatt ttatttattt tactatattg gatatatatt
    21361 cattgaaaat agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt
    21421 taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca
    21481 cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga
    21541 gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc
    21601 ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat
    21661 tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag
    21721 ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata
    21781 cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct
    21841 tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac
    21901 ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat
    21961 ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca
    22021 aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata
    22081 ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg
    22141 cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat
    22201 tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta
    22261 aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc
    22321 ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc
    22381 gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct
    22441 atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg
    22501 aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc
    22561 aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg
    22621 tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag
    22681 accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg
    22741 caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg
    22801 ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca
    22861 cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt
    22921 ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta
    22981 atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag
    23041 tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt
    23101 attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta
    23161 ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc
    23221 aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta
    23281 cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat
    23341 tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac
    23401 ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg
    23461 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat
    23521 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa
    23581 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc
    23641 agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta
    23701 cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc
    23761 tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa
    23821 gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc
    23881 ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct
    23941 gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt
    24001 ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt
    24061 tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta
    24121 gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa
    24181 taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa
    24241 taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca
    24301 cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga
    24361 ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt
    24421 aaactatgta agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga
    24481 gaaatttcgt gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa
    24541 aagtaagcag aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg
    24601 agaaggacat tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt
    24661 tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt
    24721 agaagctttg gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact
    24781 gcggcagcag ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac
    24841 aaactatcga caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga
    24901 attagaaaaa gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa
    24961 agatcaatat caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga
    25021 aacttatggc cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg
    25081 agaggatttt acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa
    25141 atagaacaaa caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag
    25201 aagcaaaatt aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca
    25261 tacaacacca cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct
    25321 tacaagattt ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc
    25381 ttgatttaaa agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg
    25441 aagaatggct tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga
    25501 cgaccatttt tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac
    25561 tgagtaagct tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc
    25621 tttcgtatgt tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg
    25681 ggttcaataa agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca
    25741 agcagcaatt atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt
    25801 gcagtggcta tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac
    25861 gagaaaaaat aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg
    25921 tagatgcaga agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag
    25981 atatatacgt cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta
    26041 aattgaaaaa ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc
    26101 caaaatatga agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg
    26161 ggatgatggt cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc
    26221 aaaaactact tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct
    26281 cgataccatc gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa
    26341 aagaactgtt tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata
    26401 cgattttgat tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg
    26461 cagcaaagga aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct
    26521 ggcaagggat cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata
    26581 cagcatttgt ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg
    26641 ttccgattat ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt
    26701 acggactgac cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta
    26761 tttcaggcgg aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg
    26821 ctcgattcag tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga
    26881 cactgctaag tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt
    26941 tttatacagg aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca
    27001 tcaatcacaa gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact
    27061 taattagtca aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt
    27121 tttcagggat cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga
    27181 caacaagtca acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggcacgc
    27241 aaggtttgta tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt
    27301 cagttgtaca aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt
    27361 tttcaggtat tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac
    27421 ctgaacatgc tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag
    27481 gtttcgttat ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat
    27541 gaacgccatt gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc
    27601 gttagaacaa gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt
    27661 tgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg
    27721 tcagctaagg aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga
    27781 atttttaaga cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga
    27841 acaaatccag tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt
    27901 cattgctgga gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa
    27961 tgaacttccc tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct
    28021 gataaacgat caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca
    28081 aggaacgatg agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg
    28141 gaataccacg aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag
    28201 acatttgaac ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc
    28261 acgatttaca gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta
    28321 aaggagcaga gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt
    28381 tggatctata cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg
    28441 acaaatcaaa atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg
    28501 aaggatgcca ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata
    28561 cgagaagtac gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat
    28621 gatcgacagt ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg
    28681 ctgacagaag tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata
    28741 ttgcagaaaa gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa
    28801 aaagaagtat ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta
    28861 gagacagatt atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta
    28921 aagcttgaac agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag
    28981 gctttcggac cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac
    29041 cttcgtttac tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg
    29101 gtgagacaag tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc
    29161 attcaaattg tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc
    29221 aattgaagaa atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc
    29281 attgagccca gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat
    29341 tcccaatcat caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa
    29401 agcagtaggt caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa
    29461 aatcataaaa gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca
    29521 agacatgtgt ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga
    29581 tgtggcgtct attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg
    29641 ctctactggt gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga
    29701 cggaatccaa cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg
    29761 cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga
    29821 agccggagaa acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat
    29881 aaaacataaa atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg
    29941 ttcattcact attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt
    30001 gacgatgctt caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc
    30061 ggatgcaccc atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg
    30121 aggagcagca gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat
    30181 tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga
    30241 gatgacggat gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt
    30301 gatggaacgt acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc
    30361 ttctatctat acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc
    30421 tatcatggca gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt
    30481 ggaagaaatg cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata
    30541 ctatgaacga tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac
    30601 cgcaatcagt gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac
    30661 attgcgggtc gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt
    30721 tccttcgatc gattggatac aaagctattc tctttattca acagaagtag ggaagtatat
    30781 ggatcatatc cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca
    30841 ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa
    30901 tgaccgtctg acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc
    30961 ttttgatgat gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat
    31021 cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat
    31081 catggacggt accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac
    31141 agaattagaa aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt
    31201 ttcagaagga ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc
    31261 cattgatggc ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta
    31321 tgcaaaatgg cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg
    31381 tccaaatatt cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag
    31441 gccatccttt agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg
    31501 gacgaccgaa agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag
    31561 aagtaatcaa tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg
    31621 caatcgatca tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg
    31681 gtcttcctca taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat
    31741 cagatgattt cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt
    31801 tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag
    31861 caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat
    31921 atcttgctta tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg
    31981 cagaggcatt gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc
    32041 ctgggtatct ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac
    32101 taaaaggatc ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc
    32161 caatccctga cttgactggt tatattacag aaggacagat catcttgact agagagcttt
    32221 acaagagtgg gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata
    32281 agggaactgg acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg
    32341 cagcctatgc acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt
    32401 tatctgatgt agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca
    32461 atcaaggatt ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc
    32521 tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc
    32581 tgccagaagg gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat
    32641 tgactcgatt gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata
    32701 agcaagatga actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc
    32761 aagaaatgga agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga
    32821 tcacggaacc tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta
    32881 tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac
    32941 agctgacaga cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt
    33001 caatcgatcg gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa
    33061 cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat
    33121 atatgacgat ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa
    33181 acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag
    33241 tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg
    33301 tttgccggct gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg
    33361 cctacttacc ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct
    33421 tttcactgca acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca
    33481 ttggaacagt gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt
    33541 tatgatggtt ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg
    33601 tattatatta aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct
    33661 ttacatccta aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt
    33721 atttgtgcct gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc
    33781 tagtttttgt aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa
    33841 tgtttatttg atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt
    33901 atggcgtgat ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc
    33961 attaaccgtt acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg
    34021 caatggggca acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat
    34081 gagtgtgaca cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc
    34141 aggtctgatt ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg
    34201 cggattgaaa acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg
    34261 acgtacgaga gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact
    34321 tactttattt tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac
    34381 tgaaacgata cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt
    34441 tggtacagta ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat
    34501 cattatagcg cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat
    34561 gacaaaagca caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg
    34621 ataatttcat cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt
    34681 ttcttgcttc ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg
    34741 ttgttgaacc tcgggtagtg cctctttttt attgaaagga atcaataaga tttgattcct
    34801 ttatttatca ataggttata attcatattt ttttattttt tcttttacga tacttagtcc
    34861 agaagctaaa atttcaaggt tattagagga tagggaaatt cgaacgaatg catctggttg
    34921 cgcacgtgta gtaaagcgtc tggaatgata aagtcggata ccgttagcaa gaaagtcatt
    34981 ctcaattatc ttagggtcaa tctcaggatc aactggaaga attcgatagt aagggacacg
    35041 attcgttggt ttttctactt tgaaaagaga atcaaaaagc tgattggatc tttttgtaag
    35101 acttaattta gtttctaaga tcctatttgc ttcatttgaa tggaggattt gagtgatgac
    35161 ttccacatct aatccagaag ttttcacatt tatattaaaa attgcttgtt ctatcttgct
    35221 ttggacatgt actggaacta caagataagc aactcgcaat ccagaacaaa taaattttgt
    35281 cattccagct aagtagattg tttggttagg taataaattt tggaaaggag gaatgatcgt
    35341 atgttgatag taagttgtta aaaatgaatg gatatcatct tcaataaccc atagaccttc
    35401 tttttcaatc acttcggcta attgctctcg tcttttgtga gagatttgaa accctatagg
    35461 attattacaa gaaggcataa gaaagacacc atgaatcttc ttttttctac attcctgaat
    35521 caatatatca ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct ctaaatgata
    35581 taactgagcc aattcaatga aattagcgta tgtatatcgg tctgttgcta ttctatcacc
    35641 tggagagaaa agtgcagaaa gtgtgatagc caatccattt tgaacaccgg aaacaataga
    35701 tagagattct aaggacggaa tgactccttg agtttttaac caatttgctg cagtagtcaa
    35761 ttgaaaattt gtgcctttgg gatcttggta attaagcaaa ccaacaagat ttgggttatt
    35821 actagttgat tgaataaacg ggacaacgaa tttattacat tcttcaaaag aactaaccag
    35881 accaaaatca atgatatgtt cggttacttc attggtagag atggtgatgg actcaaagac
    35941 attgggagaa ataaatgtgc cacttccaat attcgtatac agcaaacctt tctcaattcc
    36001 atatttataa gccttgccaa cggtagtaaa atttaaatct aaataatcag ctagctctct
    36061 ttgggagggt aactgagtgt ttttctgtaa tttattcttt aaaatatctt gttctaattg
    36121 ttgaatcaac gatcgataat aaggacgctt caatttttgt ttgtctggtc gccaagttaa
    36181 cgggtaattg tcataagaat taataggcat tttctccact ccttaaaatt gtataccata
    36241 caattttacc attgattgta ttgattttag aagttaatct tttgtgagga gggaattgtc
    36301 tatgaaaatt attaattttg aggaagtagt agcttctttt agttttgaag aagcgattca
    36361 agtaatgaaa aaatgttttt ttgattatga aagtggtaaa atcagccaaa atcctcgatt
    36421 ggtggaaatt ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc ctgcttattt
    36481 gggggagaac agatattttg gcacaaaagt cattaccgct tttccggata atcatcagta
    36541 tcatttacct tctcatttag gagagatttt cttgtttgat gctcaaaacg gactacccaa
    36601 agcattgata aacgcaaatg cagtcacctg gattcgaaca gctgctgtat ctgcgctggc
    36661 aacagattac ttagcaaatc cgcatgcaga aacacttgct ttaattggag ctggtcaaca
    36721 agcattgtcg catttgactg cgatttctac cattcgtccc atcactacag tgtttgttta
    36781 cgatatttta gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac agtatcctaa
    36841 aataacgttt ataaatgcaa tatcagtaga agatgcagta cgtccagcgg aaatcatttg
    36901 tacgttgacc tctagtaaag aggcatttct tcaaaaagag tgggtaaggg aagacgcaca
    36961 tatcaatgct gtgggcacat ttactcctga tacaagagag ataagtacat cgttgatgga
    37021 aacttctcaa atctatgtag atgattactc agctgcctta acagaaagcg gggacctgct
    37081 tatagccatg gcagaaggag atttagagga atcttgtatt gttggtacat taggcgaatt
    37141 agtttctgag aaaaaaacat tttataaagg agaatcgagg ccgacgattt tcgacgcagt
    37201 tggtctagct attgaagatc tttgttgtgc agaatatatt tataacaaat tagagaaagg
    37261 ggctttaaaa tgaacttaga ttattacgta gttgatgcat ttgcagatga ggtgttcaaa
    37321 ggaaatccag cagcggttta tgtgctagaa gaatggctgc cagaaggtac gatgcaaaaa
    37381 attgccattg aaaacaattt atctgaaaca gcatttacag tgaagaaaaa ccaagaattt
    37441 gagcttagat ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc gacattagca
    37501 acagcatttg ttttatttaa ctattacaaa atacctgatg aaacgattaa attttctact
    37561 caaagcggta atctatttgt gacaagaatt aaagattatt attacatgga ttttcctagt
    37621 attatgccga aaaaagtgcc gatattgtct gaatatgaag aagcgattgg ggctaagatt
    37681 aaagaagcct acttagcacg tgatttattt ttcgtattag aagatgaaaa aacggtggct
    37741 aaattgcagc cagattttac agctatcaaa aattttgaat taggaatagg agtcatcgtg
    37801 acagcagaaa gtgtgcaaaa agactttgtt tctagaacct tctttccaaa attaacaatc
    37861 aatgaagatc cagtatgtgg atcagcacat tctaatctta ttccttattg ggcagaaaga
    37921 atgaataaaa caaaattgtc agcttatcaa atgtcatcaa ggggcgggga tctagaatgt
    37981 gaattaaaag aagatcgtgt gataatagga ggaaaagcag ttttgttttc taaaggtacc
    38041 tcgtttattt gatttctttc aaatgaatta aaaataaatt ggtggagagt ggcaacctta
    38101 tagctacaat ggttgttaaa aaaagacgca aactttttca atttaatgaa aagtttgcgt
    38161 ctcattttaa ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc cccatactaa
    38221 ctatcctcgg atgacttcga ttttataacc atcagggtca atgacaaagt agtaagaagg
    38281 cgcagtgcca ggtaatcctt ttaagtcagt aatctcaaga ccagcagctt tatgttgctc
    38341 gtgtaatttt tctagatcat cagctgctat agcgatatga ccatagccgt ctcctagatc
    38401 atacgcacca tgatcatagt tgtaagtcaa ctctaattca tagtcatccc caggaagtgt
    38461 caaataaaca agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt cttcaaaacc
    38521 aaatgccttt tgataaaatt cgattgatgc ttcaagatct ttcactcgaa cacatgtgtg
    38581 agccattttc atagattcat ctctcctttt ccttttactc aatcataaca aaaagaagta
    38641 aggaaatagt tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg gatagaaaat
    38701 aaaactgtta aggtggaaac tattcatgga taaaccaatt tttggtgaaa aaaagcaatt
    38761 tgctgattat caaacccgtt atgcagcata tattgtcata gaaagagatc agcagattgc
    38821 agtaatcgag gctccaaatg gtgctttttt tcttcctggc ggggaaattg aaggagaaga
    38881 gacgaaagaa gaagcgattg tccgtgagct gatagaagaa atgggaattg tggcagaaat
    38941 ttcttcttat ctgggagaag ctgatgagta tttttactcg aattatcgtg caacttatta
    39001 ttataatcca ggatatttct ttgttgctga ttcttggaag cgtataggag aaccaactga
    39061 aaagaccaat aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat tgaaacgtgg
    39121 tagccaccga tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa agaaagcgag
    39181 tagatcagct ttctctcagt ttttttattt attacagtac atattttttg attgcttcag
    39241 ctacaccgtg ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg acaagagaaa
    39301 cggcatttcc cattgctact cctagaccag catattcaat catggataag tcgttctcgt
    39361 tatctccgat agccatgatt tcatcttgat caataccaag gtgctgtgcg agattcgcaa
    39421 ctgccgcacc tttacttgct tctttattta gtacttcgaa gtaatagggc gcacttttga
    39481 ccgttgtgta tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca tctaaaattt
    39541 ctggttcgtc gatcatcatc attttgacga tgttcatgtc tggtgtcatt tcttcaggtg
    39601 ttcgatattt tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa gcgctgatat
    39661 ctcgattagg cgtgtagatc gctttgtcat cgatcgaatg gagatgactg ccgacacggc
    39721 gtgccattac ttcgatctct aggaaatctt catgtgtcag tccgtgacgg gcgatgattt
    39781 tacctgattt cgtttgttgt acgagtgccc cgttataagt aataacgtaa tcattatctt
    39841 gaaaaaggtt caactcgttt aactgttcct ggacacctgg taagggacgg ccagtgcata
    39901 aaacgatcga aacacctttt gcaacagcag cggcaatcgc ttcttttact tcttttgtta
    39961 cttctctttt ctcatttaat aaagtaccat cgatgtcgat agcgactagt ttgatagaca
    40021 taatttcctc caatatattt taatttattt caatcaatgc gccattacgg atatggctgg
    40081 caaattcttg ataagtttcg tcaaataaat cataatgatc acgcaaggac ggatcaacca
    40141 tttcttttgg aaagaaaaat cgctcatctc cttgttcttg tcccgctaat gcagcaacaa
    40201 gctgactaac ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc tcgatttgtg
    40261 tgcgatgtct gccttgttct gggcgataaa aatcataagg tagatcgtag ctagaattca
    40321 cagcagtgta ataaacagga ttatatccga ccttttctac taacaaagtg agttcttgga
    40381 ctaattttga atcccggttg ccagaaaaag tagcagattt cagtggttta cgattcaaaa
    40441 aacgtttagc taaatcattt aaaataggat cagcagattc agaccattga gtaaagtaag
    40501 tgcccaagac cccatcatca agtttgaggt attcttctaa agtaaagctt ccgtcaagaa
    40561 atggcagaag taattgagaa tggagttcaa aactgctggc atcttcttga tacaactcgt
    40621 gtgcgcgatg aagcagatgc tccaaaatta cttccatacc acgggaaaca ggatggaagt
    40681 acacttgcac atacatttga tatcggctaa cgatataatc ttccactgca tgcatgccac
    40741 tcatggaaaa agcgatcccg ccttcataag gacgaatcac acgtaatatt cgggtaagat
    40801 caaatgttcc atattctgtt ccagtgaaat aagcatcccg aagcaaatag tccattcgat
    40861 ctgcatcgat ctgactagaa atcatttgga caacttgtgg gttggggtaa gttttttgga
    40921 tcacactagc taccttttca ggaaaacctt cttctactcg gttaagaatc tgatacactt
    40981 ctgtttctgg agaagtgatg atctctacag tgatggcttc atgattcgta tggaagatat
    41041 gttcaaatgt atgagagtaa ggaccatgac cgacatcatg caacagtgcc gcacataaag
    41101 tcaccagccg ttcctgatca tcccagcctc cgttgcctat tttttctttt gaaaaatttc
    41161 gagaaaagat atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa tgagtaaaac
    41221 ggctatgttc tgctccgtga aaagtgaaag aagaagtgcc taattgttta atgcgtcgta
    41281 gacgttggac ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg acatggatat
    41341 agttatggac tggatcacga aaaacttttt ccataggcag catttgatat ttataagggg
    41401 ttgtcataat gtagctcctt tatgttatta cgttgttcca ttctctggat ttggttgttc
    41461 cattgctcca gctggatttc tttttttagc cagtcggtag aatcctgtaa atcacttgca
    41521 gaatacagct tttcaaaagc ttttgcaaat gcttctttga cttcttttac aaaaaggggt
    41581 tggtcaagca gttcttcaag tgttgccatt gtagcaggat ccactgcagg atagccattt
    41641 gtaccaaagt cttcttttaa agcagattga taaaatcgct tcatgacttg cccacgtttt
    41701 tcctgattac cgttgacact taagtacatc ataacagaaa tcccattttt gatccgacgc
    41761 tgagcgatac cggcaaattt tttgccatgg atgcttaaat cataagttcc aggacaataa
    41821 gaatgggtga tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt agtcaatagg
    41881 agcatttgct cgtaagcgtc atctatggtt attttttttg tgtcactttg ggggaaaatc
    41941 aaagaaatat tcaaaattcc ttcatctgat acgatcccta atccacctga atttctgatc
    42001 actggagcgt aacctgcatc ttccagtgtt tggatcccgt cacttagaaa aggagttcga
    42061 acatccttca tacctaagat gactgtgcag tcatattgcc agaaatgtaa aaagatttgc
    42121 tggtgttctt tagaatattc cgttaatata tctgttaatg caaaagggga aaagtcgttt
    42181 ttttgataaa tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc tcctttgtat
    42241 aaattcatat tttctacaat attatactct aaaatcaaag agttgttttg gaaaaaaggt
    42301 tttctgtaaa aatggacaag ttggttgaca aaacaaaaga aactaggaac aatgtagcta
    42361 tgagaagtaa aggagaatga ataatggaat tatcacaagg aacaccgatt tcaatcaaat
    42421 tgcgtacaaa agtaaaacag caaggggaag tgcaagattt ttattttgat ttgaaagggc
    42481 aaatggtgac aattggcgat acgctgtaca tacgttataa agagattcag gaggagacgg
    42541 gcgatgagat tcctgtaacg atcaaattag ttccagatgg tcatgtacag ctgatccgtg
    42601 caggagaaat gcggatgcgt ttgaaatttg gctataaaga acggttagaa acatcctatc
    42661 gaacaccata cggtatgatc cagattgcta cttttacgaa agaactgcat gttagtttga
    42721 aagaccgtcc cacagcaggg aaggttcgaa tcgattatga tctgttcatg ggaccggaaa
    42781 aaatcggcga atattatttg accttggatt ttacagcgta aatataaaag aaaagtttga
    42841 ttttttgtgt ctgattttgt atgataagga gtagactgtg aaaggacgtg tcctgattgg
    42901 aaattagtgt atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa gtagcacacg
    42961 cgatcttaga acaacgtggc gatgtcatgg atttttctga tttagctaac cagatccaaa
    43021 actatcttgg gaaatcagac agtgaaattc gtgattcact cgcacaattt tatactgact
    43081 tgaatattga cggcagcttt atctcattag gggataatcg ttggggcttg cgttcatggt
    43141 atgctatcga ttcgatcgat gaagaagtaa accatggaat ggacgaagag gatgaagata
    43201 ctccacgtcg ccgtaaacgc aagaaagtca acgcctttat caatgatgat gaagacgcga
    43261 tcgattacaa tgacgatgac ccagaagata cagatctgac agaagaagat gacgatgatc
    43321 tgtttgatga cgatgatgat gaagatgaag aaatcgctgc ttataattct gacttgcaag
    43381 aaatcggagc agatgacccg acagatgatg aagaagactt gccaggaaat atcgaagaag
    43441 atttaagtat catagacgaa gacgatgatg atgaagatta tgaagaggaa gaattttctg
    43501 actctgaaaa agaataaaca ttattttctt acgagaggtt gtgacagaga cgatcagcct
    43561 atcagaataa ggaggtgtga cacgattttt
//
LOCUS       DACECZ010000009        41696 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.009, whole genome shotgun sequence.
ACCESSION   DACECZ010000009 DACECZ010000000
VERSION     DACECZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.009"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            252..743
                     /gene="nrdR"
                     /locus_tag="IWO35_000535"
     CDS             252..743
                     /gene="nrdR"
                     /locus_tag="IWO35_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ0315616.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            766..2139
                     /locus_tag="IWO35_000536"
     CDS             766..2139
                     /locus_tag="IWO35_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ0315617.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            2139..3065
                     /gene="dnaI"
                     /locus_tag="IWO35_000537"
     CDS             2139..3065
                     /gene="dnaI"
                     /locus_tag="IWO35_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ0315618.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            3194..3634
                     /locus_tag="IWO35_000538"
     CDS             3194..3634
                     /locus_tag="IWO35_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ0315619.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            3778..4479
                     /locus_tag="IWO35_000539"
     CDS             3778..4479
                     /locus_tag="IWO35_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ0315620.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            4476..5597
                     /locus_tag="IWO35_000540"
     CDS             4476..5597
                     /locus_tag="IWO35_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ0315621.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            5612..6844
                     /gene="pepT"
                     /locus_tag="IWO35_000541"
     CDS             5612..6844
                     /gene="pepT"
                     /locus_tag="IWO35_000541"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ0315622.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            6976..7884
                     /locus_tag="IWO35_000542"
     CDS             6976..7884
                     /locus_tag="IWO35_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ0315623.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            7919..8191
                     /locus_tag="IWO35_000543"
     CDS             7919..8191
                     /locus_tag="IWO35_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ0315624.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            8202..9248
                     /locus_tag="IWO35_000544"
     CDS             8202..9248
                     /locus_tag="IWO35_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ0315625.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            9499..12108
                     /gene="clpB"
                     /locus_tag="IWO35_000545"
     CDS             9499..12108
                     /gene="clpB"
                     /locus_tag="IWO35_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ0315626.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(12259..12930)
                     /locus_tag="IWO35_000546"
     CDS             complement(12259..12930)
                     /locus_tag="IWO35_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315627.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(12923..13417)
                     /locus_tag="IWO35_000547"
     CDS             complement(12923..13417)
                     /locus_tag="IWO35_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0315628.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(13437..14657)
                     /locus_tag="IWO35_000548"
     CDS             complement(13437..14657)
                     /locus_tag="IWO35_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0315629.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            14804..16639
                     /gene="lepA"
                     /locus_tag="IWO35_000549"
     CDS             14804..16639
                     /gene="lepA"
                     /locus_tag="IWO35_000549"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ0315630.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            16835..17746
                     /locus_tag="IWO35_000550"
     CDS             16835..17746
                     /locus_tag="IWO35_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0315631.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGQLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            18745..18996
                     /locus_tag="IWO35_000551"
     CDS             18745..18996
                     /locus_tag="IWO35_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315632.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            19079..19351
                     /locus_tag="IWO35_000552"
     CDS             19079..19351
                     /locus_tag="IWO35_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315633.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            19441..19668
                     /locus_tag="IWO35_000553"
                     /pseudo
     CDS             19441..19668
                     /locus_tag="IWO35_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19826..22126
                     /locus_tag="IWO35_000554"
     CDS             19826..22126
                     /locus_tag="IWO35_000554"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAQ0315634.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTATQPTVEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPVT
                     SQPVEETASITTPASETSTPEGEKAAEATPSVKPESSEKQAEEKQRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIEDQKKKEQDIPKTGTPTLLVTVP
                     TNRSVRVRRATNTAVLGGRKIDWGENIGVTSSPNNPNFTLNYMVRFEINGQVAFCIEP
                     GVSAAKGQVYTATALENYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLLNNFDVRATFHNQTHTIKIGETLKVKSGNDFLQRV
                     KGIQKPKGINVSIHGNEAWITVTKEAPEKAKIHFNQIEVLGTPLAYRRPGAQTIGILN
                     PFEPGDSVVNLTILKQGNIRVFKEDAETSVKAQGASSLNNAVYGLYQADGKKIKEVTL
                     KNVNGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLIDSMNPDNKPKLAGIEISLTSKTTGKVVKTVVTDKDGYATFGKNAV
                     VFDTYILSETKGKEGYKLFEPFEVTISENGQTFHYALEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTFTYGKDRYRLEE
                     IKAPVNYVLNQNHWYSRLPMIQNQSK"
     gene            22084..24108
                     /locus_tag="IWO35_000555"
     CDS             22084..24108
                     /locus_tag="IWO35_000555"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAQ0315635.1"
                     /translation="MVFSITNDTEPIKVIHFANRQARKNVKLFKYEEWNKKKTPLAGV
                     TFTLYNQAGKALGEYITDQNGEILVKGLVANNYYFIENTPLESFQPNTDKQAFTITFK
                     QNAPDDGETLLVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGK
                     EYTISGVLMNKATNTPILQDGKEIRKTLTFIADKVNGTKDLEFVVNASVLKGQTIVVF
                     EKLFRDGIEVAAHANINDENQSVRVNNPDVHTTAVFEDGLTVADPTGLFTLNDMVKIK
                     EIIPGKAYLVKGILMDKATGEALLVHGQPVESAGGFIAKAENETVIVPFTFDLTGLNG
                     REIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKVVNPLMKITI
                     TDAVKYHDLIVGKTYTVNGILMDKSTEKPVLVDDKEITSTLTFVAERIDGTVNLDFVL
                     DARALRGKEVVVFEDLFRDGVLLASHADITDKDQTIKIVNPTITTKATNKNGGKEILA
                     LPNQTVLEWVKVDGLAIGQLYKLLVQGYLTPDGTPFEGTHAEKVFTADKEMMEFLFEF
                     LVDGRNLAGKGLSFVEWLQAKTEDDKEAFEEVVKHNVDLTNKDQTVQFVEAPKPPQPV
                     KPEQPVKAATVVPMLPKTGEQTSSILMVLGLVVLVIVLGITLYKVRKAKD"
     gene            24207..24572
                     /locus_tag="IWO35_000556"
     CDS             24207..24572
                     /locus_tag="IWO35_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315636.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIEEVL"
     gene            24574..25026
                     /locus_tag="IWO35_000557"
     CDS             24574..25026
                     /locus_tag="IWO35_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315637.1"
                     /translation="MGLKFPNNYDITEALDETCVGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNLKQSQEKGLDQKQPSVDSKEKEKK"
     gene            25095..25472
                     /locus_tag="IWO35_000558"
                     /pseudo
     CDS             25095..25472
                     /locus_tag="IWO35_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25641..26951
                     /locus_tag="IWO35_000559"
     CDS             25641..26951
                     /locus_tag="IWO35_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ0315638.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMRWSRLKEGYFASVYWRQLLA
                     RMLIDNRFIYTKKQVGEKQMKEKMRLPKVYFYQKKQELVISFPLDGGRFHERFLKMGN
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     LLISGAIGGGKTYLLYSLIKACLDVGTVDICDGKAADLAALGDVDIFKGHVFYKTNEE
                     MIACLRNALKEMNQRYAYMKTMKEPKYEPGKNYAYYGISPHFIFFDEWAAFYGSLDME
                     TRNKVDKLIQPLVLKGRQAGMYVILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            26974..27285
                     /locus_tag="IWO35_000560"
     CDS             26974..27285
                     /locus_tag="IWO35_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315639.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVGLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            27645..28901
                     /locus_tag="IWO35_000561"
     CDS             27645..28901
                     /locus_tag="IWO35_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0315640.1"
                     /translation="MTPIELRNYRKKLSAIHASGGGKKLLSQKRFAQLLGCSVDYIKK
                     MEQGKRKIPEDFEQRIKERFRLYVESGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDERWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQSYLEWMGTSGGKIA"
     gene            28898..29443
                     /locus_tag="IWO35_000562"
     CDS             28898..29443
                     /locus_tag="IWO35_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315641.1"
                     /translation="MNSYMTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLESIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLATVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            29444..29944
                     /locus_tag="IWO35_000563"
     CDS             29444..29944
                     /locus_tag="IWO35_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315642.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKRELEGQFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            30024..30581
                     /locus_tag="IWO35_000564"
     CDS             30024..30581
                     /locus_tag="IWO35_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315643.1"
                     /translation="MSLEFLGEPIEKEEFVLHYMDLFIQLIKKLCAIDEFYPREQDYL
                     AAERIKISLLWEKFTDENNRPPDYRYLSDCLTNNVLSKEYLFIKREKNQMSVEHFCDC
                     YIALLRSQQLLTVERYGAEFGYVMQAERHRATRCYEKEDQVIDGYEELCIRNNRYLQQ
                     RLYEQLMMDFSNLYRGYECSMIQER"
     gene            30601..30946
                     /locus_tag="IWO35_000565"
                     /pseudo
     CDS             30601..30946
                     /locus_tag="IWO35_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            30965..31879
                     /locus_tag="IWO35_000566"
     CDS             30965..31879
                     /locus_tag="IWO35_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0315644.1"
                     /translation="MIKTTLAVVTTVGITLFSFQLGTLLVNADETVETPKSEIVDTLP
                     GEELPDVETELSGGASETPEQSQPEQPEKELIESEPEFPESTLPTDPIEEEGVHGSTT
                     TDSSTVTTPEQSGTTSSSEKTTDSSSTEQPTMSSTSEESKEEPSQPAEKASEKSEAIP
                     KENKPVVPAKEVTVSVTPSGEITTNTSQGTSVPIVTSNVEELTHIPTPTTPLKVESGQ
                     TIVGVKDGIPLVQDSEGNLVKDLSIPVKKLPSGNIEVKTADGKTKVLPKTGEEIHVVL
                     SVLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            31958..32950
                     /locus_tag="IWO35_000567"
     CDS             31958..32950
                     /locus_tag="IWO35_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0315645.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKGVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKIDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTLVITKKTNQYYVEKLTHNWLDTKGEERK"
     gene            32947..33165
                     /locus_tag="IWO35_000568"
     CDS             32947..33165
                     /locus_tag="IWO35_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315646.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNNPTRVFDAFGKIIEKLFMN"
     gene            33178..33588
                     /locus_tag="IWO35_000569"
     CDS             33178..33588
                     /locus_tag="IWO35_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315647.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVAI
                     FEYLFLRSFISWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYVV
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            33641..36169
                     /locus_tag="IWO35_000570"
     CDS             33641..36169
                     /locus_tag="IWO35_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ0315648.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVLNLIWLDVAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGTSYLALLPIGETPENMRSCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATIIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSFVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVEYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILTHLRLEVNEANLSEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            36185..38395
                     /locus_tag="IWO35_000571"
     CDS             36185..38395
                     /locus_tag="IWO35_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315649.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYLLLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVINPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQSSKQQGNNQRKPLLNKGIAFVGQSIPLPAGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPKSSVATLSGNRSVQQLGQRSSESKPSISAP
                     KSLQPAFSSLDKSTDQSKKIEKDPKTLPILDAKLGTDFTLPKGRTVQKPLSKKG"
     gene            38403..39494
                     /locus_tag="IWO35_000572"
     CDS             38403..39494
                     /locus_tag="IWO35_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ0315650.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEDGGEATGLAVAFNGE
                     YTEGLPIFSEIRGRGSITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYQGAMSIIKSYNLTEYDTFAIKHWNSLPTKK
                     SSLVAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YDWTAYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKMK"
     gene            39515..40057
                     /locus_tag="IWO35_000573"
     CDS             39515..40057
                     /locus_tag="IWO35_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315651.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            40279..40509
                     /locus_tag="IWO35_000574"
     CDS             40279..40509
                     /locus_tag="IWO35_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315652.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            40523..40855
                     /locus_tag="IWO35_000575"
     CDS             40523..40855
                     /locus_tag="IWO35_000575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315653.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            40900..41448
                     /locus_tag="IWO35_000576"
     CDS             40900..41448
                     /locus_tag="IWO35_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0315654.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            41455..>41696
                     /locus_tag="IWO35_000577"
     CDS             41455..>41696
                     /locus_tag="IWO35_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315655.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYS"
BASE COUNT        14167 a         6633 c         8931 g        11965 t
ORIGIN      
        1 tttttgtgct agatgactaa atcagctttt gtgtaagtta tttctcagta atcaaggtga
       61 tcagtattgg aaaattgaaa tttagtaagg acaggggaca caattacgca tgaaagtaga
      121 aaaaattgtt cttatatccg tgttttttgg gtaggacagt cgcgtttgtg atttgtttcg
      181 tgttataata caactagttg taaagaaaga cacatccttt atataaaatg cgatgcaaga
      241 ggtgagacaa aatgagatgt cccagatgtc aacataataa ttcacgagta atcgacagcc
      301 gtcaagcaga tgatggacgt gcgatccgtc gccgtcgcga atgtgagaat tgttcgttcc
      361 gattcactac atttgaacga attgaagctg ctccgttatt agtaatcaag aaaaacgggg
      421 aacgcgaaga gttcaatcgc gataaaattt taagaggact catccgttca gcagaaaaaa
      481 gacctgtagc tatggaacaa atggaacaaa tcgtcaacca tgtcgaaaac cgcgtacgca
      541 gtctcggaga aaacgaagta tcgacgaatc taattggtga atatgtgatg gaagatttag
      601 ttgatttaga cgaaatcgct tatattcgtt ttgcaagtgt ttatcgccag ttcaaagata
      661 tgtctgtttt cttaaaagag ttgcaagata tcgtggataa agcaaaagaa aataataaag
      721 agaataataa agaaaacaat taagaaacaa aaaggaggca tttccttgga aaatgcttgg
      781 aaagagcttc aaccaaaaaa tatttaccaa ggaagaaaag cggctccgct gagagaagac
      841 agcaagaacg ccttgctgta tctttatcag ccactaatcg gcggagaagc cttgagtatc
      901 tatttcaccc tgttatcaga aatctctcaa gaatctggca aaggtcctga agggatgcat
      961 gcagatctgc ttagtagtct aggctgcggt atcccgcaat tttatgatgc aagaaaaaag
     1021 ctagaaggaa tcggcttact ggatgtttat tttaaagaag atagtgatct gggacctcgt
     1081 tttatttatg agctgttaga accactcatt cctaaagcat ttttcaaaga ctctgttctg
     1141 gcttttcttc ttctggaaaa agttggtgaa cgacgattcc agaagcttac ccaattcttt
     1201 gaacctaaaa ggcatacagc agagggatat caaaaaatca caaaaaaatt tttagatgtt
     1261 tatcgcttca atgaagcagt gtacgctgct aatcaagaaa acatcgaatc gctgaataaa
     1321 tcttttgaag aatctgaatc agtagcatta cttgaagcag attcgacatt cgattggcag
     1381 tttttgaatg attggctggc aaaaaaacat attgcaagtc ttgatgatcc aatcatcaaa
     1441 caagtcgaca tgtatcatca gctttacggt ttcgatgaat tgacacttgg cgaactgatc
     1501 agtcaatctt atgactttag tgaacaaaaa gtatctgaaa aagaactgca aaaagccatt
     1561 ttggcaatgc agagacgtcc ggtgaagcaa acaaaagaaa cagttgcggt ttctgaaaac
     1621 agtaatgaac cctcgtcaaa cgaacatttg acaaacgcag cgcttcagct gataaaagaa
     1681 gcacagagca ttccaccgat gaaatatctg gaagcaatca agtcagaaaa aggcggatat
     1741 gtttctaaac aagaaaactg gttactgcaa gatcttgttt ctcaatcagg tctttccagt
     1801 agcgtgatca atgttttgct gaattatgta ctagtgatca agaataatgc tagcttaaat
     1861 gccagttatg taaatacagt ggcaaatgaa tgggcacaaa agaaaatcgc cacagcagaa
     1921 gaagcgatcc agcacattcg gcaaataagt aatcaagcaa aacaatcaaa acaaaagaag
     1981 caaacaaatt atcaaactgg caagcgaaat gttcgacggg aaaagcttcc tgattgggtc
     2041 aaccagccaa aagatgaaaa acagatcagc agcgagaaaa aagcagagat tgaccgccgt
     2101 tttaaagaat acctggcaaa aaaggagggt gatatctagt ggaagacgta ggcaaagaat
     2161 tgaagaggat catcagcaaa cgcaattatc aagaaaagta tgctgaaatg atcgctgaag
     2221 tcttaaatga tgcggatgtt caagcttttc taagcgcaca ccaagaagaa ttgacacaag
     2281 cggatattga aaaaagttac gctaaattgt atgaattcgt gcaagaaaag cgtaaatatc
     2341 aaaaaaacga tccaacgatg atcgctcccg gctatgagcc gaagctgacg ttgaattttc
     2401 attatgtgga tgtcacttat gtaccaacgg aagcattgtt agcacggcaa agacaagaag
     2461 aaatcagaag ccgagtaaaa gcaatggata tgccaaaaga tattcaatcg gcttcttttg
     2521 aaaactttga acgtacagat ggacgaggat atgcgagctt agaagcccta gattttgtag
     2581 agaaatatac tgctgatcca aaagggtatc ataaaggact gtatctagta ggaagttttg
     2641 gtattggaaa aacatattta ttaggagcaa cagcgagaga attagcgatt gctggattca
     2701 ccacgacgct agtccatttt ccgacatttg ccgttgagat gaaacaggca atcggaaaag
     2761 atcaagtggc agaaaagtta gatgcagtga aacgttcacc tattttgatg atcgatgaca
     2821 ttggtgcaga tgcgatgagt agttggatac gagacgaagt attcggtgtt atccttcaat
     2881 atcggatgca ggaacagtta ccaaccttct tctcatcgaa ttttacaatg gatgaattgg
     2941 aacagcattt gtccgtcact caacgtggag atgaagagcc gctaaaagcg aaacggataa
     3001 tggaacgcat acgttatttg acaaaagaaa tcgaaatgac tggtcgcaac cgacgcaatg
     3061 gttgaacagt tgacaagtcc aactgacatt tgacctagta gtaaaagcag gtcagcgaat
     3121 cattatatgg acagaaatga ccggagacaa tcaccgcaac aagtaatgca gaaacttaaa
     3181 cggaggattt tttatgaggg ctattttttc tagtatatct gtagttggtc tattttttct
     3241 tttagcaggc tgcggtagcg cagatagcaa cacaggttct gttgattcga ttgatgcggg
     3301 tcaaacagta aaaacaagca gtacgaaaac ggagagacaa agcatatttg tcggaacgat
     3361 tgaaagtatt actcaagtag aagcagaaga agaaacgctt cagttgaaac tcgtcgatgt
     3421 catggccaaa gaagatccag cgaatatggg aacgtcattc agcaatgacg gagtgatctt
     3481 aaatgctact gctgaaaaaa taagcgtgga cataaatgaa ttgaaaatcg gtgacaaagt
     3541 ccaatttaca ttagttgaga tgccgatcat gacgatgtct atcccaccgc aagttcctgg
     3601 agattcgatt attgatgttt cattgaacaa ataaccaata acattttttc aaaaataaga
     3661 aaatgttgtt ttatagaacg aaaaggtata ttttttaagg agatatggga atttttttat
     3721 tcctagtctc cttattttat ttggaaaatg ctataattat ctagaaggag tgaagctatg
     3781 aaccatttag atttatctca gcgcttagcc gcagtgggtg actttgtccc tgaatccgct
     3841 cgtttagcag acattggatc agatcacgct tacttacccg ttgcattgat attaagagga
     3901 aaaatcgaat atgcagttgc tggtgaagtc gttcaaggtc cttatgaatc agcacgtaag
     3961 caagtcaaaa aagatggatt agaagaccgt atcaccgtac gccttgcaaa tggtctagat
     4021 gctatccagc cacaagatgg tattacagct gtcacgattg cgggtatggg aggttcactg
     4081 atccgagata tcctagaagc tggcaagttg aatcaacgct taagcggaca agaacgtttg
     4141 atccttcagc caaacatcgg agagcgtaca ttacgatctt ggctgcaaga aaatcagtat
     4201 aaaatccaat cagaaaaaat acttgaagaa aataaaaaaa tatatgaaat cattgtagcc
     4261 gaaaaagaac aaaaaccagt tacttacaat gaaatggagc tgatgttcgg gccatttctg
     4321 cttgaagaga aaagtgagat cttcaaatca aaatggacaa gagagcttca acaaagaaaa
     4381 gctatccgtg aacagctcaa acaagcagct aatcaagagg cacgtcaaaa acaaatcgag
     4441 caggaaatcc aaatgattga ggaggtttta aagccatgac aatcagcgga cgcactttca
     4501 tccaaaagtt tgaaagctat tgtcctcagt ggctagctga agaaggagac ccagtcggac
     4561 tgcatatcgg aacattggat aagccaatcc aacgagtaat gatgacatta gacgttcgtc
     4621 cagaagtggt cgaagaagca atcaagaaaa aaatcgattt gctgattgct aaacatcctc
     4681 caatctttcg tcctgtaaaa cggctggtta ctgatcagcc acaagaaaaa atgtatgcag
     4741 atttgttgaa acatgatatt gccgtctatg cagcacacac gaacatggat attatttggg
     4801 atggactgaa tgactggttt tgtgaacttc tcggtattga agtggagagt tatttagtaa
     4861 agactcatga gattcattat aaaaaactgg ccgtttatgt acctgtggat catgcacaga
     4921 aaatgcgtga agtattagct gcagctggtg ctggtacaca aggtgattat accggtacaa
     4981 gttttacctc gattggacac ggtcgattta ctccggaagc tggtgcacaa ccagcgattg
     5041 ggaaagtagg gaagacagaa caagttcaag aggcaaaagt tgaggttatt ttgccggaaa
     5101 ctatcgaaaa acaagtgatt caagccatgc ggtcagctca tccttatgag gaacctgctt
     5161 atgatctttt cgctatagat gaaccagtcg aaatgtttgg gttaggacga gtaggtgaat
     5221 tgccgcaaga aatttctata gaggcatttg tagaacaagt aaaagaagcg ttccaactag
     5281 atggactgag aatagtccag ccaaaaaatg caaaatcctc tgtgaagcgc attgcgattt
     5341 gcggtggaag cggtgaaaaa ttttatccgc aagcaatcgc ccaaagagca gatgtctata
     5401 tcacgggaga tatctattat catacggctc atgatatgca aagcgctggg ttgattgcaa
     5461 ttgatcccgg tcattatatt gaatcgttat gcaagcaacg atttatcgag aaatttgaat
     5521 cttggaaaca agaagaaaat tgggatatta actttttcgt ttctgaaaca aatacaaacc
     5581 catttcaatt taattaaaag gagagaatac tatgtacgaa aatcttttgc cacgtttttt
     5641 acgatatgtc aaaacagaaa cacgctccga tgcaacaagc aaaacgactc catcgactca
     5701 gacacaagtt gcttttgcgc aagaattgaa aaaagaatta gaagagttag gcttagctga
     5761 tgttttctat aatgaagata acggattcgt tatgggaaca ttaccaagca atgttgatca
     5821 tgaagtgcgt tccattggat ttatttccca catggataca gcagatttta atgcagtagg
     5881 agtcaatcca caaatcattg aaaactatga tggcgagtct acgatcgtac tagatgcgga
     5941 agaacgctat acattgaata caaaagattt tccaaatttg aaaaattatg aaggccacac
     6001 attgatcaca acagatggca gtacattact gggagcagat gacaaatcag ggattgcgga
     6061 aatcatgact gcaatggaga tcttgatcaa taatccttct atccctcacg gtaccatcaa
     6121 agtggctttt ggtcctgatg aagaaatcgg tgtcggagcg gataaattcg atgttgagca
     6181 attcgatgta gattttgctt atacagtcga tggtggtcct ttaggtgagt tgcagtatga
     6241 aacgtttagt gctgctcaag caaatattac gatccaaggg aaaaatgtac atccagggac
     6301 ggcaaaagat acaatgatca atgctctaca gttagcaatc gattttcata atcagcttcc
     6361 agccgatgaa gttccagaaa aaacggaagg atacgaaggt ttcttccatt tgatggcatt
     6421 ggatgggaat cctgaagaag cttctatgag ttatatcatt cgcgatcatg atcgtgaaaa
     6481 attcgaagca cgcaaagcac aaatcacaca tatccaagaa gagttgaatc agcgatttga
     6541 tcaagaacga atcaaagtag atatgtatga tcaatattac aacatgaaag aaatcattga
     6601 aaaagatatg agcatcgtgg aattagcgaa acaagcaatg atcgagcttg gtatctctcc
     6661 agtgatcgaa ccagttcgtg gaggaacaga tggttcaaaa atctcttaca tgggtattcc
     6721 gacgccaaat ctatttgccg gaggagaaaa tatgcacgga cgattcgaat ttgtttcttt
     6781 acaagtaatg gaaaaagcaa cagacgtgat tgtaaaaatt gcagaattga acacaaagag
     6841 ttaaaatcga aaaagaacag cttatgacaa ggcgttcgtc ccaatgatgg aatgtcatct
     6901 gtctaaggct aaagggcagg acaatggaat tggttgtccc gccctctttt ttgaagagta
     6961 aataggaggt aaagaatgga aaagctgcta gttttttata acaaaagttc agggaaagat
     7021 gagggtgaga aattagcacg ctggtttcaa gattatgtaa aagaaaagca gccagaatta
     7081 tatgtagaac tagctgaaac tggcccatcg attgatgatg cggaattagt caagaaagca
     7141 gaagccatag aagctgatac tttagtaatt ataggtggag acggcacgat tcatcatatc
     7201 gtccaagcgt tccaaaaaac attatctcaa taccatgtcg gattacttcc aggtggaact
     7261 gtcaacaacc ttgcacgtgt tcttgagatt cccctcaaaa aagaagaagc tgctgacgtg
     7321 atcctggaag gcaatgtacg gaaaatagac tatggtcaag tcaatgatga agtcattatc
     7381 tctacactga caatcggaat cttagcagat acagcagttt ggatcagtca agaagaaaaa
     7441 cagcgttacg gctcgtggat ctttatcaag aagtttttta agttattgct gaagaagaaa
     7501 aaatatcatt tggatttaga aacagacgag gagcattgga aaggaaaatc ccaactgttg
     7561 acagtcacga tgtctaactc cgtgggcggt tttacaaatt ttgacgactc agcgacccta
     7621 gatgatggaa aatttcatgt aacgatcgta ccttccttag atattttccg atttattttt
     7681 tatttgccta gaatcatgag aggcaaattc tatacgatcc ccggcattac ttatttcaca
     7741 gccaaacgca tgaaaatcca agcgcaggaa aaatctgtgg gcactcgaac ggatggagac
     7801 acgacagatg atcttccagt tgaattatct gttgtatcaa aaggattaac agtttattca
     7861 ccaaagaaag aagaaaaagc ttagaaatag tagttaacag aaagttggaa gaagagtaat
     7921 ggatgtattg ttacacaatc ttttgtggtt ttaccttttc attgtgaatt tttacttatt
     7981 ttgtctgatg gggtatgata aacatcaggc tagaactagc ggctggcgtg ttcctgagaa
     8041 taatttgata tttgtcagcc tgttaggcgg tggtttggga ggattgatct ctcgaaaagt
     8101 tttccatcat aaaactcgga aaaagaagtt tactatagga ttcgctgcag gtgtacttgt
     8161 tgatttgatt ctcatttatt ttttccatta aggagcagga gatgtcgtct aaaggaacga
     8221 aaactaaaat cattctgaat ctgcttttgc tgattgtgat ttttgccatt atttattatt
     8281 taatcaatca atcatttgca gatatctttg ccgaattgct gtctaccagt gttgttgttt
     8341 tggcagcggc tgttatatta gggacagttt accaaatcgc tgaagggcgc tcaatcaaag
     8401 aaattgctga acctttcgcg ccgaatttta caacgaaaga tgggttcttg acctctagct
     8461 atattgcgtt ttaccgaatc gtttcgtttg gtacaggaac gctgatctcg gagatttatt
     8521 tttataacaa aaaaggaatg aagctatccc agggagcagg ggtgacagca ctccatatga
     8581 ttatgtacaa actggcagtg atcacttatg cattagctgg cttggtcatc caattctctc
     8641 ttttttattc taatggtcca tcaatgatct ggtttatcat cgctggtatc cttttgacga
     8701 tcgtgattgt tgccttttta ttggcgatgt ccgtcagctt gaatgttcaa atattttttg
     8761 tcagcttatc gaataagtta tttaaaagtg agcgtttgcg gaaatgggtg gatacatgca
     8821 atcagcagat ctactcttta agggaaacgg tccaaaccat tatccaagac aggactgcgc
     8881 tattgcggat ttatctatgg aatatgttaa aacttgtatt ttggtatgta cttccttatt
     8941 tgattctgat tgaaaaccat cctaaaatag attttctact gaccatttcg tttatcagct
     9001 ttgcagtagt gctttcaggg gttattccta cgcctgctgg tattggttcc tttgaatttg
     9061 tctatttgtt attattcaga ccagttgttg gaacagttga cgcagtttct tcgcttttac
     9121 tgtatcgttt cagttccttt atcttgccat ttctctatgg atttgtgtat gtgattgctg
     9181 aacggcgaag agtgatcaaa gaagaaataa tagaagtaaa aaaagagaat aaagaaaatc
     9241 aagggtaggt cttttataag atctaccctt tttatcaaat caaagaaaaa cgttatggat
     9301 aagataattt tacgtttgtt tggctctttt tttatataca tacaatcaat tttgtttttt
     9361 taagatttat ataagtcgag aaaaaaagtc aaaaaaccat gtgtatattt tgaccaaaat
     9421 tgaccaaagc gatataatag tttttgtaaa gatcaaagaa ataagtgatc ttacctttaa
     9481 ttaggagggt atacctgtat gaatattgag aaaatgacta cgacgcttca agaagcgatt
     9541 gctgaagctc aacagattgc ggtcactcga aaacatcaag atatcgacat tgcccatgta
     9601 tggaaaatct ttttacaacc taatcatttt ggtcgaaatt tctatacgga tgccggattg
     9661 gatgtcgaat cttttgaaca tgaaattgat cgtctactgg atgagtatcc agttgtttct
     9721 ggtggaaacg tccaatatgg acaaaacctc agccagaatt tctttcgatt attgaatgaa
     9781 gcagaccaaa tacgtgaaag cttcggcgac gagtttcttt caacggaagt cgttatttta
     9841 gcgttgatga aattaaaaaa ttatccgtta actgtttatt tgaataaaaa cggattatct
     9901 gaaaaagaat tgcgtaaaaa catcgaagag atgagaggag gagaaagagt gacctcacaa
     9961 aaccaagaag aacaatacaa agcattagaa aaatacggcg ttgatttagt acagcaagtc
    10021 aaaagcggaa aaatggatcc aatcatcggt cgtgatgaag aaatccgtga tgtcatccgt
    10081 attctttcac gaaaaacaaa aaataatcct gttctgatag gagaacccgg tgtagggaaa
    10141 acggcaatcg ttgaagggct tgctcaacgt atcgtacgca aagatgtgcc tgaaaattta
    10201 aaagataaaa cgattttttc attagatatg ggtgcattga tcgctggagc aaaattccgt
    10261 ggtgaatttg aagaaagact aaaagcagtc ttgaaagaag tcaaaaaaag tgatggacgt
    10321 atcctcttgt tcatcgatga aatccacaat atcgtagggg caggaaaaac agaaggaagt
    10381 atggatgccg gaaatctgtt gaaaccaatg cttgctcgcg gtgaattaca tttgatcggt
    10441 gccacaactt tagatgagta ccgtcaatat atggaaaaag acaaagcgtt ggaacgtcgc
    10501 tttcaaaagg tattagtcaa agaaccaaca gtggaagata cgatcagtat tctgcgtgga
    10561 ttaaaagaac gttttgagat ccaccatggt gttaacatcc atgacaacgc attagtagca
    10621 gcagctactt tatctgaccg ttatattact gatcggttct taccggacaa agcaatcgat
    10681 ttgatcgatg aagccagtgc aacgattcgg gtagaaatga actctatgcc aacggaatta
    10741 gatcaagtta ctagacgttt gatgcagttg gaaatcgaag aagctgcact gaaaaaagaa
    10801 tcagatgatg caagtaaaaa acgcttgaaa aatttgcaag aagaattagc tgaacttcgt
    10861 gaagaagcaa atgcgatgaa gatgcagtgg gaaacagaaa aagaagaagt caattctgtt
    10921 tcagcaaaac gtgctgaaat cgataaagca aaacacgaat tagaagatgc agaaaataac
    10981 tatgatttag aacgcgctgc tgtattgcgt catggtacga tacctcaatt agaaaaagaa
    11041 ttgaaggaat tagaagctaa agcaaaagac agcgagatta agatggttca agagtccgtt
    11101 accgaaaatg agattgctca agtcgttggc agattaacgg gtatccccgt aacgaaactt
    11161 gtagaaggtg aacgagaaaa actaattaaa ttgaatgaaa cacttcataa acgagtgatt
    11221 ggtcaagatg aagcagttga tgcggtcagt gacgcggtaa ttcgttcacg ggctggattg
    11281 caagacccaa atcgtcctct aggttctttc cttttcctag gaccaactgg tgtcggaaaa
    11341 accgaattag cgaaagcgtt agctgagaac ttgtttgact cagaagatca tatggtacgg
    11401 atcgatatga gtgaatatat ggaaaaacat gctgtgtctc gtctagtcgg agctcctcca
    11461 ggctatgtag gttacgaaga aggtggtcaa ttaacggaag ctgtcagacg aaatccttat
    11521 acaatcgtct tgttggatga aatcgaaaaa gcgcatcccg atgtcttcaa tatcttgtta
    11581 caagtcctag atgacggacg cttgactgat tcaaaagggc gagtagtaga tttcaaaaat
    11641 acggttctga tcatgaccag caatatcggt tcacagttac tgctggaagg cgtgactgct
    11701 gatggaacga ttccagaagc agtggctgaa caagtcaaca cgctattacg tggaaacttc
    11761 aaaccagaat tcttgaaccg aatcgacgac acgattttat ttacaccatt gagcctagac
    11821 aacgtgaaag gaatcgttga caaaatggtg gcgcaattgg cacaacgtct ggaacatcaa
    11881 gaaatcctgc tgacaatcag tgatgaagcc aaaacgtgga ttgctgaaaa tgcttatgaa
    11941 ccagcatatg gtgcaagacc attgaaacga tttatcacta aggaagtaga aacaccacta
    12001 gctaaagaaa tcgttgcagg acatgttatg cctaaatcaa aagtaacgat tactttactt
    12061 gatggacaac ttcatttcaa aacagaagag ctagaagaaa tcgtctaatc ttccattgag
    12121 actataaaag aaaaaagggg tctgacacaa ctagtgtcag acctcctttt ttcaaataaa
    12181 cagtgttcca gaagtagttt cttcgaaaaa taagccgaaa tttcttgaag ctgcccgctt
    12241 ctgtcacaac ctcttttatt aggtattttt cactttttct aatttataac tttcaaagta
    12301 acgctcatct tttttacttt gagaccatgc aaacaatccc attaaggcag cacctatcgt
    12361 atttgtcagc aagtcgccca tcgtatccat caatgctgct cgtccgacaa acagtgtgcc
    12421 atcagaagca gcaaaacgtt gaagattcat gcctaagaac tggtcgcata agaattccca
    12481 gaactcccaa aacacgccgc aaagtccagc aaacgcaaag ccgaataaga ggaacaacca
    12541 aggactggtt tttgatatct cagcatcttt catcaagtaa ccaatcaatc catagccaag
    12601 agctgtcagg accattggac tcaccgcatg taagattttg tcccaaaaag agatgatact
    12661 gatcaaatgc atccctgtgc ctaaaaagac agagatgaac aagaaaaacc agtaaaacaa
    12721 aacgattgct gctggtattt taagtttgaa aactttcaaa atgacttgtg gcatgaaaat
    12781 caatgcaatc cctaataaag attccacgat aaatgtgatt ccttgagaag tcgaatattt
    12841 ttgtgtgata aattcataaa aattatagca gacaacgaat acaccaaaag caagtaaagc
    12901 tatttgcacc tttttgattt ctttaatcac tgaaagtccc tccatctaaa aatcttttta
    12961 cttctgaaag caactcaatt tctgaagaaa ccacttttcc gttcaatttg atcaaaccta
    13021 ttgtatataa gttcaggtag tgaaattgat tttcagctgt ttcttgtagt gcagcgattt
    13081 ttttcgggtg ggttccgcct tgctgtcgag tatcagtata taagccgatc actgggatgc
    13141 ctttcgcata agccacaccg atttccgatg ctaccccggc atcgatcgtc agcccatcta
    13201 gtaaagcaat cattaaatca ctagccaaca ctttttctgt gtcagcaaga gcaatcattc
    13261 gactgtctgc ataagcagtt ttgtcattga ttgcttcatt ttcttgagga agatagatcg
    13321 aaagctcaga agagagctct ctgatttctt taactaactg ttggttatat aacaaatcac
    13381 ttttagcaaa cattggtgcg gcaaaataaa ttttcatcta aacatcatcc tttttatcaa
    13441 aaataatcgc taaagtaagc ttccatgttt ggaatgatac gctctagtga cttcagtgtt
    13501 tcttcatctg tttctaaccg ttcgattctt gccaaataag aagcacggcg ataattcatc
    13561 atcaagcaag tcaatgtttg gatatccagt cgaacggatt ttcctatagg ttcatttaag
    13621 acacggactt gtccctgttc atcccatgtt aaagcgaaga caccattatt ccaaggagcg
    13681 acaggatctt caatgatgaa atgaaaagca tcagctgttc caacaaacgg aaaccgttga
    13741 aggaattctt ttacatcaac gatccgagcc ataaaatatg gttcgatctg ctctttgatc
    13801 tgactatctt caatcaaaaa tgctaaaggc tcatttttat aaatatctcc atgtacccag
    13861 tagatcatgg aaaaatgtgc ggaaataaag ttccataagc catttcgagc ttcttgatca
    13921 agatagaaca tttcttttac gtgaaatatt tcttcagcta cccagtagaa cagaacacct
    13981 cttggttcat ggttgctatc gtagtagaca gcggctgtgc gctcatcttc attttcgaaa
    14041 cgccaatact cttcccaatg aaaatcactg cggaataaag caccatggtt ttgttgggca
    14101 aatttgtcat aaacggaaaa tacgtctgga tgatcaactg gtaatcgctc aaccatacca
    14161 cttactggga tttgtttagg tagctgtgta tctctgattt taaaggaaag tttatcagac
    14221 atgatttccc atcctttacg ccgatagtag gggatactgt aaggatacaa ataagagatc
    14281 cattgcttat tcttgcgcat attatccaat gcaacgataa ttagatcttg catcaatcca
    14341 tggttagcat attctggata agttccaact ccagtcacac cacccatttt ataacgtgca
    14401 ccgtgaatat tgacttcaca aggatagata gcgatttgag agatcagttt attttcgtgg
    14461 aaccatccga acactttcga tacttccaaa atcggttttt tggaacggat aaaagctcgt
    14521 ttattctcaa acccgctttc ctcgatgtct gcttcagtca cttggaacac ataggaaagc
    14581 aattcattga attgatcgat atgttcttga gtgaccggtt ttaattcaag attttctcta
    14641 aaatcttttt gatccatttt ttcatctcca tttattagct tgataacagt atagcaaaat
    14701 tctacaaaaa aaacttctat agctgactaa ttaaaaactc tttaaattac tgcttttcgt
    14761 tggtataatg gaaagtgctg attatatgaa aagaggagac gctatggata taaagaaaat
    14821 gaaagaacgc caagagcaga ttcgtaattt ttcgatcatc gctcatatcg accatgggaa
    14881 atctacattg gccgatcgta ttttagaaat gacgcacact gtgacgtctc gcgaaatgca
    14941 agatcagctg ttagactcga tggatttgga acgtgaacgt ggaatcacga ttaaattaaa
    15001 tgctgtagaa ctcaattaca cagcgaaaaa tggagaaaca tatatctttc acttgattga
    15061 caccccaggg cacgtcgact tcacctatga agtctctcga agccttgctg cctgtgaggg
    15121 tgccgtttta gtggtcgatg cagcacaagg aatcgaagca caaacattgg ccaatgttta
    15181 tttagcgtta gacaatgatt tggagatcct gccagtcatc aataaaatcg acctacccgc
    15241 agcagatccc gaacgtgtcc gtcaagaaat cgaagacgta atcggaatcg atgctagtga
    15301 agctgtatta gccagtgcca aatcagggat tgggattgaa gatattctcg agcagatcgt
    15361 ggaatatgtt cctgcaccaa gtggagactt agatgcaccg ctaaaagctt tgatctttga
    15421 ctctatttat gatagttacc gcggtgttgt tctaaatgtc cggatcacgg acggtatggt
    15481 caaacccgga gacaaaatca aactgatgag taatggcaaa acatttgatg ttacagaggt
    15541 cggggtcttc tcgccaaaag cagttgctag agactttttg atggtcggtg atgtcggtta
    15601 tatcacagca agtatcaaaa ctgtccaaga tacacgagta ggggatacag taacgttagc
    15661 agacaatcct gccgctgaag ctttaccagg ttaccgaaaa atgaatccga tggtttattg
    15721 tggtctttat ccaattgata cttctcgtta caacgatttg cgggaagctt tagaaaaact
    15781 gcaattgaat gatgctgctt tacaattcga accagaaacc tctcaagcat taggttttgg
    15841 gttccgctgt ggattcctcg gacttcttca tatggatgtc gtccaagaac gcttggaacg
    15901 ggaattcaat ctagaattga tcacgactgc accatccgtt atttaccatg ttaataaaac
    15961 ggatggtacg actgctacag tagataaccc ggcagatttt cctgaacctg taacgattca
    16021 ggatgtagaa gaaccattcg tcaaagcaca gatcatggtc ccaaatgatt ttgtcggtgc
    16081 agtaatggaa ctttctcaac gaaaacgggg agaattcatt acgatggatt atttagatga
    16141 ttaccgagta aatgtcgtct ataatattcc gctttctgaa attgtctttg actttttcga
    16201 caaattgaaa tcaagcacga aaggctatgc ctcattggat tatgaaatgt ctggttatca
    16261 aaaaagcaag ttagtgaaga tggacatctt gctgaatgga gaaaaagtcg atgcattaag
    16321 ctttatcgtt caccgagact ttgcctatga acgtggaaaa gcaatcgttg aaaaactgaa
    16381 aaaactaatc ccacgccaac aatttgaggt gccaatccaa gcagctattg gacaaaaaat
    16441 cgttgcccgt tcagacatca aagccttgcg taaaaacgtc ttggctaaat gttatggtgg
    16501 agatgtttct cgtaaacgta aactcttaga gaaacaaaaa gaagggaaga agcggatgaa
    16561 acaaatcggc tccgtcgaag ttccgcaaga agccttcatg gccgttctga aaatggatga
    16621 gcaagatagt gtgaaataac ttataatcaa tcgaaaaacc tattaaatca acctttttga
    16681 gtagatgcaa cactgaggat tatctgtata aaagatttta aactgtcatt tggttatcac
    16741 ggcgatacta aaattaacaa aattggaata tttttcttga agatttttag ctgtcagtat
    16801 atgatgaaag catctcatag gaggtgcttt tttcatggtt gataattcaa gtggaagggt
    16861 tcttaaatct ctaagaaaag agaaaaaact atctcaaaaa aagttagctg atttagcagg
    16921 tatttcacaa agtactttag ttaaatatga aaaagggtca agaaaaatcc ctaaagacgt
    16981 agataatact ctaagcaaga ttctgaatat agaaactcta ataaaagatg aggaagataa
    17041 gattgagatt ctcatcggtc aactaattgc atacagagat atgaataagt tacttaacaa
    17101 agagttagct gataatatag gaattagtga agtattactc agttatgtgt taaatagaaa
    17161 gagaaatccg tcaaaagaaa tgcaaaaaaa aatcgatatt tttctattaa gtaatgaaaa
    17221 agaaattttg aaggagatta atcgtgatag tgagatattt agtttatcaa aagacgataa
    17281 aattgttatg ggaaaaagaa tacgagaagt ccgaaagaac cgggaagaga cgcttgaaaa
    17341 atttggtaaa aactttacca tctatacagg caaaaatgtt ataagtagat gggaaaaagg
    17401 tattaatatt ccagatattg aaaaactaat gaatattgcc tatcttggta aggtcactgt
    17461 tccttacctt atgtacggag aagattataa gaatatactt ccaaaagatg aaagggttag
    17521 cgattttaaa aagataaatt cttttagtat gggattaagg atgaggaaaa ttaggaaaga
    17581 ttattatcta gaaagggagg agtttggaaa gcttttttct ccttcaattt caaaatggtc
    17641 catagatagg tatgagaatg gtagagatat acccaatact aatcgaatta tccaatatgc
    17701 ttatatagga aatctttctt tagaattttt gatttacggg atataaaaat agtttacgtc
    17761 gatacttatt aagttatcga cgttttttta ttatagaaaa aatttataaa aggaggtttc
    17821 ttgatatgat aagaaacaaa aagtgttggt tgaaagttgc tttactggca gctttttttc
    17881 taagtaactt tttgacaagc tcaatggcaa cacaagcgga agaagtaata caagaaataa
    17941 ctgttatgaa taacagctag ataaaatggg ctaggtatgt tatccaacaa tttagtaatc
    18001 actagaagcc tagcgaaagt tcgcttgata agttatagat gagtattgat ttgtagtgtt
    18061 tccaaatcaa aagggtacag agccacatga aatatgttgt cctctgcagc gtcaggatgg
    18121 gaatccatac catggagatt cggagatctg tgagggaata cttacgtggt taatatgtcg
    18181 gttgtgttgg acgtagtaat ggtcatagtg ggtagattga ctgaattctt atatgagaaa
    18241 atgaaaccaa caagaagata gggttattta tacagaattg gggatctttt ctggaaaagt
    18301 aatcggacaa tgcgagattc gtacggtctt tcttaaattt ctcttaaaaa aaacggcatc
    18361 gaatgggttg ggtgattgat aagcaatgaa tcatagactg atatagaata aaaaatgatt
    18421 ctatttatct ttttacatct atcgtttttt caagtaaccg caaattgtat cagcattcaa
    18481 caatttttgt atacaagttc tagtaaaagg atctgaagaa aaagttggtt gttgtgaaga
    18541 taaaaaaaga atcaggtttt actgagtttt tcttcaaaat ttgtgttatt aggttaggac
    18601 aaagataaga agaagtatga agatttaaaa aaggaatgag gaaaagatag taatttccga
    18661 ttattggagt ttttttattt ataaagaaga agcgaataag ttgtgattcc tagatttcgc
    18721 gcgtgattaa ttaatgagca aaaaatgcgg aaagttttag tgtttaatac acgaggacaa
    18781 agtttcttag gtattgaagg cacacgtgta acggttgatg ggtggtgtga ttgtttcgag
    18841 gttggaaaac atgttgaact tatggcattt actgagctaa aagataagaa caacgtggaa
    18901 tttttcgaga gtgatattgg ttgggacgat catgaagaag tttatggaaa atttattttg
    18961 cggaagggaa attcttatac gaatggggaa gcataagtag cgatttgtta gagatcattg
    19021 ataggattga aattgtcggg aatatttatg agaatctaga gttatttgag gtgatagaat
    19081 gagtgatggt tttgaacaag tatcaataat taaaaatcaa gttcgggaga ttcttagaaa
    19141 aaaaggctat ttggtagata gttattttga aggagattac gaaacatggg taggtgtcta
    19201 tgctcgtcca gagaataaac ctacctattt agatcctacg acgtcagaag atgcctattt
    19261 acaaaatagg tatcgagtag atggttttaa gcaagacttt gctgagtggt ttgaatggga
    19321 aattgaaaac ggagaagtaa aagaagaata agcgtacttg ttgtatcaaa ataagccagt
    19381 agaacaacca aaccaatagc tagtaatgac aataatactg aggaatcgga tcattatggt
    19441 gtgattgata tttctcttcc agaagatctt aagccagatc ctgcaacaat tggcaacagt
    19501 ggcatgattt ttgattcgtt agatgaagct atgtcatatg gttggaaaga aattaaggat
    19561 gaaggtagta attggtatgg catgagattt ggtgatggtg aagttgccaa taaagctggt
    19621 attccaattg ggaaatggac agttgatttc cacgaagcag gttctttttt gttctgtaat
    19681 tatagtggga gggtaaaatc attgatgaaa tgtgttcaga taagagcgcc agataaaaat
    19741 agaagagaca gttcacgtcg ttatcacttt tggtaacggc gttttgtttt attatataaa
    19801 attctcagga aaaggaggaa ttcttgtgta tgcaaaaacg aaaatgaatt ggttgaaagc
    19861 actcgcattg gtggcctttt ctcaattctg tttcttggtg ggaactgctc aagcacatgc
    19921 agaagaaacg acggctacac aacctacggt ggaagccatt gcccaagtcg cttctgaagt
    19981 gacagaagtc caaccagcag aagccggggt cccagctggt gaagccactg ttgaacaggc
    20041 ccctgtgaca gaagcgccaa cggtcaattc caaaacgact ctcgatcaga cgattgggga
    20101 aacacaacac ccagggaata gtcctgttac ttcacaacca gttgaagaaa ctgcctcaat
    20161 tacaacccct gcttcagaaa cgtccactcc agaaggagaa aaagctgccg aagcaacgcc
    20221 ttctgtgaag ccagagtctt ctgagaaaca agcggaagaa aagcaacgaa tagaagaaac
    20281 agtggaacaa ccagcggcta ctgctgacaa agaaggaaaa aatgaggaaa acgttgaaaa
    20341 aacaatctct tcaaccgcag ctagtcaaaa tcaaccaacg aaaccaaaca aagaatctac
    20401 ggctagtatt gaagatcaaa agaaaaaaga gcaggacatt ccaaaaacag gtacacctac
    20461 actgttagta acagtaccaa ccaatcgttc ggttcgtgtt cgccgtgcaa ccaatacagc
    20521 tgttttaggt ggtcggaaaa ttgattgggg cgaaaatatt ggtgttactt ctagtccgaa
    20581 caatccaaac ttcactttaa attatatggt tcgttttgag attaatggac aagtggcgtt
    20641 ttgtattgag cctggtgtct cagccgcaaa aggtcaagtt tatacagcta ctgctttgga
    20701 aaactatttg aaaaattcaa atgtacggaa aaaaacaagc ttgatctctt atttaggata
    20761 tgtcacaaat aaagacaaat ccgatgaaca atacattgcg gcacaattta tgatttggga
    20821 agctttgggc acaaaaatcc caatcaaaaa tatccgtgtt gattatgatc gtcgaaaagc
    20881 agaaattcaa cgtctgctta ataattttga tgtaagagcg acgtttcata atcaaacaca
    20941 tacgataaaa attggcgaaa cgttaaaagt aaaaagtgga aatgactttt tacaacgtgt
    21001 aaaaggcatt caaaagccaa aaggtatcaa tgtatctatt catgggaatg aagcatggat
    21061 cacagtcaca aaagaagcac cagaaaaggc aaaaattcat ttcaatcaga ttgaagtatt
    21121 gggtacgcca ttagcgtatc gtcgtccagg tgcccaaaca attggtatat tgaatccttt
    21181 tgaacctggt gactctgttg tcaatttgac tattttgaaa caaggaaaca ttcgtgtctt
    21241 taaagaagat gcagaaacta gtgtgaaagc acaaggtgcc tcttctttga acaacgccgt
    21301 ttatggtttg tatcaagccg atgggaagaa aatcaaagaa gttactttga agaacgtcaa
    21361 tgggaaagtt caagcagaaa ttaaagacct tgatcctggc aaatatgttt taaaagaaat
    21421 caaagcgcca aaaggttatg tgctttctga tgaagcaatt gaaattgttg ttgaacctgg
    21481 aaaaactgtc aatgcgaccg caaaagacca agtgattaag ggcaccattg atttagtgaa
    21541 agttgctaat aaagacttaa tcgattcaat gaatccagat aataaaccaa aattagctgg
    21601 tattgaaatc tctttgacgt caaaaacaac tggaaaagtg gtaaaaacag ttgtgacaga
    21661 taaagatggc tatgctactt ttggtaaaaa tgccgttgtt tttgatacgt atattctctc
    21721 tgaaaccaaa ggaaaagaag ggtataaact atttgaacca tttgaagtca caatttctga
    21781 aaatgggcaa actttccatt atgctttaga agataaagta atcgaacaac gtcttaaagt
    21841 ggtgaaagtg gatcaagaaa caggaaaagt gattccactt gccaatacag aattcaagat
    21901 ttttgatacc ttagccaatc gttatgtcac aatggagatt ccaaatgata cagaaacaac
    21961 ggatatcttt aaaaccaatg aaaaaggctt ttttctaact aacggtacat ttacttatgg
    22021 aaaagatcgc tatcgtttgg aagaaattaa agcgcccgta aattacgttt tgaatcaaaa
    22081 ccattggtat tctcgattac caatgataca gaaccaatca aagtgatcca ttttgcgaac
    22141 cgtcaagcac ggaaaaacgt gaaattgttc aagtatgaag aatggaacaa gaagaaaaca
    22201 ccgcttgctg gtgtcacctt tactctgtat aaccaagcag ggaaagctct tggtgaatat
    22261 atcaccgatc aaaatggtga gattttagtg aaaggtctag ttgccaataa ctattacttt
    22321 atcgaaaata caccattaga aagtttccaa ccaaatacag acaaacaagc attcactatt
    22381 acattcaaac aaaatgcgcc agatgatggc gaaacgttat tggtttctgt acgtaactac
    22441 ttgattccgc caaaactaga aacacaagcg tcaaataaag ccgatggtga aaaagtggtt
    22501 gatccattag aaacgatcgt cattaaagac agtgtttctt ataccaacct gtttgtcgga
    22561 aaagaataca ccatttcagg agtattgatg aataaggcaa cgaatacacc gatcttacaa
    22621 gatggtaaag aaatcagaaa aactttgacc tttattgcag acaaagtcaa tggcacaaaa
    22681 gaccttgaat ttgtggtcaa tgccagcgtc ttaaaagggc aaacgatcgt tgtctttgaa
    22741 aaacttttcc gtgatggcat tgaagttgcg gcgcatgcga atatcaatga tgaaaatcaa
    22801 agtgttcgag taaacaatcc agatgttcat acaaccgctg tatttgaaga tggattgacg
    22861 gtcgcagacc caacggggct gtttacctta aatgatatgg ttaagatcaa agaaatcatt
    22921 ccaggaaaag cttacttggt caaagggatc ttaatggata aagcaactgg tgaagcctta
    22981 cttgttcatg gtcaaccagt agaaagtgct ggtggcttta ttgccaaagc agaaaatgaa
    23041 acggtgatcg taccatttac ctttgatttg acaggtttaa atggtcgtga aatcgtcgta
    23101 tttgaaagtc tttttagaaa tgatgaagaa ctggcaaacc acaaagacat caacgacaaa
    23161 gggcaaacgg tacgagtaac gaacccaagt gtacgtacaa cggctacgca taaaccaact
    23221 ggcttgaaag tggttaatcc attaatgaaa atcaccatta cagacgctgt gaaataccac
    23281 gacttaatcg ttgggaaaac atatacagta aacggtattc taatggacaa atcaacggaa
    23341 aaaccagtgt tggtagacga caaagaaatt actagtacgt taacgtttgt ggcagaacgt
    23401 atcgacggta cagtcaatct agactttgtg ttggacgcac gtgcgttacg aggaaaagaa
    23461 gtcgtggtct ttgaagatct cttcagagac ggtgtattat tagcttctca tgcggatatt
    23521 accgacaaag atcaaaccat caaaatcgtc aatccaacca ttacaaccaa agctaccaat
    23581 aaaaatggtg gaaaagagat cttggcttta ccaaatcaaa ccgtgttgga atgggttaaa
    23641 gtagacggtc ttgcgattgg tcagttgtac aaactgcttg ttcaaggcta cttaacacca
    23701 gacggcacgc catttgaagg tacacatgca gaaaaagtct ttacggctga taaagaaatg
    23761 atggaattct tatttgaatt cttagtggac ggtcgcaacc ttgcaggaaa aggcttaagt
    23821 tttgtggaat ggcttcaagc caaaacagaa gatgacaaag aagcgtttga ggaagtcgta
    23881 aaacataacg tggatttaac aaacaaagat caaacggttc aatttgtgga ggcaccaaaa
    23941 ccaccgcaac cagtgaaacc agaacaaccg gttaaagcag ctactgttgt accaatgttg
    24001 ccaaaaacag gcgaacaaac aagtagtata cttatggttc ttggattagt agtattagtc
    24061 attgttttag gtataacact ttataaagta cgtaaagcca aagattaggc atgaggaaca
    24121 agcggttagg atttttaacc gcttgtttga ttgtaatgta gaaatattta gaatttattt
    24181 tggtttttaa gaggaggaaa aactttatgg cagaaaaagc attgcgtatg aagaaagagt
    24241 ttttaggtca agtattttta ttgggttcac gggaacctaa tccaccaaag gaaattaatg
    24301 gtgtgtctgg ccgatatgtt caattacaag cagaatgttc gcgagaagaa cagcctgctt
    24361 tttttgttta tctcacagct gaagaggata aaagattacg attaccagaa aaagaggtgc
    24421 gtatttcatt tgaagaagct tattttgttt ttcgtaaaga acgagtgaat agtcgtgaaa
    24481 caaatgatat gggacaaaca gagcgtcgaa caactggcta tcaagatgtt ggtgtctttt
    24541 taactggcgt aaaaattgag gaggttttgt aagatgggat taaaatttcc aaataattat
    24601 gatattacag aagcattaga tgaaacttgt gtaggagccg tacgctttgg cgcattagaa
    24661 gagtctgatc gtgtatttga taatgaaaca ggcgattata cgggagaaat tcgatcacag
    24721 cgagcacaat tagtttttgg aaatccagat gaaccaaaga ttgcacgacg agttaaattt
    24781 ccagcagatg tagatttgac tggttttaaa cgacgtgatc ctgtgaaatt aattgatcca
    24841 gaatatcatg tggtatatat tccagcacaa gatggcaatc gtgaatattc agatttagtg
    24901 atttatgcga aaggattaga gaaagtagaa gctccaacaa ataatcctaa tctgaaacaa
    24961 tcacaagaaa aaggtttaga tcaaaaacaa ccaagtgttg attctaagga aaaagagaaa
    25021 aaataaggat tttttgaaag agtaggaaga agtcttccta ttctttttat ctaaggtaga
    25081 aggaagggga gattttgaat tcatataaaa ccaatcaaac acttcgagag attgtggctt
    25141 gttgtcaaaa tacccaacaa ttgattgagt attttggaga caatccgacg atttttgcac
    25201 aaacaaatgc atatatttgt gcggttattg ggcagtgtat gtttttacgt gatcttgttc
    25261 gtttttcatt accatatcag tggaaacaaa atcatcagga agaggtttgg attcgtgtag
    25321 tacgaatatg taatatgtgt gcacacccat aaaaaaatcg attagatcgt tttcaattat
    25381 ggttttttat taaatgtgat attccagtat tagttgcttt ttgtcgaaag tttttagcac
    25441 aggagaagta taaacagaaa ggaggaatgt gacaattgga acctcatttt tttaagtatc
    25501 gtggtcggcg aattcgttat ttcaatcgtt atttgtggtt ctgttattat ggtttctttt
    25561 tactcccatt tttgattctg ttaggttggt ttagttggaa agtattttat ccgcgtttaa
    25621 gtacgtattc gaattggcaa atgtatctta ttcctggaat aagtattgtg ttgtatttat
    25681 tgctggttag tggattaatt ttaggtttga tgcgttggtc gcgattaaaa gaaggatatt
    25741 ttgcctctgt ttattggcgg caattacttg ctcgaatgtt aattgataat cggtttattt
    25801 atacaaagaa gcaagttggt gagaaacaaa tgaaggaaaa aatgagatta ccaaaagttt
    25861 atttttatca aaagaaacaa gaattagtga tttcttttcc tttagatggt gggcgtttcc
    25921 acgaacgatt tttaaaaatg ggtaatttgt tatctgaagt atttttgcgt gatttaattc
    25981 gtgaagaaag agaaaaagcc cgaatcaatt atgtatttct ttccgattct ggtcggattc
    26041 ctattgatca atgtatagca gaaaatggac gtattcattt aatgaaaacc ttggattggg
    26101 tatatgatca atcgccaaat ctgttaattt ctggtgcgat tggtggcgga aaaacctatt
    26161 tattgtattc attgatcaaa gcttgtttag atgttgggac agtagatatt tgcgatggaa
    26221 aagcggcaga tttagcggct ttaggtgacg ttgatatatt caaaggacat gtgttttaca
    26281 aaacgaatga agaaatgatt gcttgcttac gaaatgcgtt aaaagaaatg aatcaacgat
    26341 atgcctatat gaaaacgatg aaagaaccta agtatgaacc agggaaaaat tatgcttatt
    26401 atgggatatc gccgcatttt atcttttttg atgaatgggc agctttttat ggttcgttgg
    26461 atatggaaac aagaaataaa gtcgataaat tgattcagcc attagtatta aaaggtcgtc
    26521 aagcaggcat gtacgttatt ttagcgatgc aacgaccaga tgcggaatat tttccaagtg
    26581 gtgttcgaga taatttaacg ttccgtattt ctgttggtcg attatctccg attggctatt
    26641 taatgacttt tggtgaagat tttaaaaata agccatttta caacaaagat caacctggtc
    26701 gtgggtatgc ttttccagga tttgatgtac ctgtagagtt ctttgcgcca tttgtttaca
    26761 aagactatca ctttatcaaa gaatttgaga aatttactga aatgattgtc tatgatttta
    26821 cagtaaaaga ggatagtcaa gatcaaacga tgggagatga taatcagttg ttacaagaac
    26881 aactagagcg ccaaatgtat ttagcaaaag atccaaaagg aacaagtttt tctgatgcgc
    26941 atattttgta atcaactaag gaggtgaagt taaatggcag agagttgtta ttatcgagta
    27001 gctacagcaa tacgaatgat taatccttct ctttcttcca gaacgtttta tgattggttg
    27061 aatcgtattg aacaagtaac tgattaccgc tttttgcgca aagaacgtgt ttttacagga
    27121 aaagttatta atcaagtatt gctaacgaaa aaagatatag aacgattgac tcgattgtat
    27181 cattatcgag taggtttaga agaggattta actttgtcaa tttatcgcgt tttttctcct
    27241 gaaaaatata gtgaaataac aaaattagat catttaattt tgtagttatt atctatcggg
    27301 agggctctag acgtagtctg gtagccgcac tctgcctggc ggtgcgcatt aaggctgcga
    27361 agcatgggcc tataatgcgc actcgctagc agcgtgtgca gctcacaaag aggtcgtgcg
    27421 cttgcgcacg gcctctttgt gtaaagcccc ccgatatata atatgggggg cagaaataac
    27481 ataacacaaa taaaaaagta tatttaaagc agttatatca aggctttagc taaagttaga
    27541 agaagtaccc ctttttagaa aaatcagggg gtaccaccta tgataccggc atttctagca
    27601 gattgaaaaa atcgaaattt gttattttat aaaggaggta gaatatgacg ccaattgaat
    27661 tgcgaaatta tcgaaaaaaa ttgagtgcta ttcacgcttc aggtggaggg aagaagttac
    27721 tctctcaaaa acgttttgca cagttattgg gatgctcagt agattatata aagaagatgg
    27781 aacagggaaa aaggaaaatc ccagaggatt ttgaacaacg aatcaaggaa cgctttcgat
    27841 tgtatgtgga aagcggaata gatcttcgag caatgattga ttatttgcgt ctttcctttt
    27901 ttgggacaga ctacaaaaca gtgattcaac gtgtattacg aatggatctg acagaatttg
    27961 aaacacgtcc aacgggcttg tatctttttg actgtctttc tgttcgtggg aatatctggg
    28021 tgttctggca ttcaaaacga accgtaacag aaaatgtact tgtacaattt tcaggacaag
    28081 gttgccggga atatgaggaa gtcatgcgtg aacaacaaac agattggcga acatggctat
    28141 attctatttg gaaaaatcaa ggaaagttgg aagctggtta tacacgtgtg caatgtagtc
    28201 gattcgatat tgcgttagat gagctttggg gattggaaac ggaagaacat ttcgatctct
    28261 ttgaattact agaaaaacaa aaagcgggtc tattagaaat ggattttaag agattccaga
    28321 atattggcgg ttgtttttta gatgatggct attttcgaag tgaaggatta tccttgtatt
    28381 ttggatcgcg aaagagtcca ttgtttttca atttctatga aaaacgtttt gaaattgcga
    28441 accgagaaaa aatttcagaa ttagaagcat taacgaaata tgggatttat aatcgatatg
    28501 aattacggct agccaatgag aaagcaacac aagcagtaga agcatttatt ttaggtgatt
    28561 caccgaaacg tttaggagag attggcgttg gtttaattaa tgcaaagctt attgtttggg
    28621 atcgactgga aagtgatgca cggattgttg atgaacgatg ggcaaaattg tttcgttcga
    28681 tggaagaatt ggattttaca gtaaagccag aaaaattctc attagatcgt tcagaacgct
    28741 ggtttttgaa acaagtagct ccgacattaa aattgttaca agaagcagat cgtattttag
    28801 gaagagactt tgtacaagaa ggcattgata atgctgaact aaatgacgag caacaaagct
    28861 atctagaatg gatgggaacg tcaggaggga aaattgcatg aactcatata tgacaacaga
    28921 atggttgcgc ctgttggatt taaaagctgc gatcgaagca ttaaatgaaa agatggttga
    28981 tttgtcttat tttcgttttc gagttcctta tattgaacaa gcggtaaaag ctggacgtta
    29041 tcaggaaaaa gagaactggc aagaaattgc acgcttatta gaagttcgta agggatacga
    29101 acaagaactc gaagagttgg aatttagccg aaggaaaggt aggctagaat ccattcgctt
    29161 ttatcgattt tcattgccaa tacctgcgat tttagcggtt aaaaaaggct gcgataaaat
    29221 gaagatatac gaaaactgtg tggcagcact tagcagcgaa aaacctctta tggaagaaat
    29281 ttcattggcg actgtcactt gggaaatgag tcgccagcca acagaagaac agacatatct
    29341 ttctttagaa gagattgaga tagaattaga ggaaattggt cgctatgcca cttgttctac
    29401 ctattgtgga tcggtaatta gtattgctgg tgtgattgtc taaatgaaaa aaattatgat
    29461 ttatgttggt gcgtattggt cacgtgatcc aactgtatta gaaaatccag aggcagtaca
    29521 ctattgtttg cgccaattat tctatctgta taaagaacga ttagaatcgt tgattcggca
    29581 gttgccatat actgacagac gtttagatga actgcttcta aggtatccag caatgtataa
    29641 acgacgaaaa aatagattgt taccagaaga gtatcccatt gaaaaacgtg aacttgaagg
    29701 gcaatttgtg gcttatttct atgatgatgt tcgtatgcgc ttagtagaac aacgaatgga
    29761 aatagaaaat gatcgctact attttatcaa ttattgcaag aagcgtaagt atcaagtaac
    29821 tgatgatttt tatgattgta ttttacaaga tggggaagtg attctatcca aaattgctcc
    29881 aagttttcgt gaacttgtgc cgattgattt tttgaaaaaa tgtcatttgc ggattcttcc
    29941 gtaaaaaaga gctataaaag gaaagaaaag tagaagccaa atcggtttct gcttttttat
    30001 tttttaagga agcagaagat ggtatgtcac tggaattttt aggagagcca attgaaaagg
    30061 aagagttcgt tttgcattat atggatttgt ttattcagtt aataaaaaag ctttgtgcga
    30121 ttgatgaatt ttatccgcgc gaacaagact atctagccgc tgaacgaata aaaatcagtt
    30181 tattatggga aaaattcaca gatgaaaata accgaccgcc agattatcgc tatttatcag
    30241 attgtttaac gaataatgtt ctttccaaag agtatctatt tatcaaacgt gagaaaaatc
    30301 aaatgtcggt tgaacatttt tgtgattgtt atattgcatt attgcgtagt caacagttat
    30361 taacggtaga acgatatggt gcagagtttg gttatgtcat gcaagctgag cgtcatagag
    30421 ctacaagatg ctatgaaaaa gaagatcaag tgattgatgg ctatgaagaa ttatgtattc
    30481 gaaacaatcg ctatttgcaa caacgtttat atgaacaatt gatgatggat ttttcaaatc
    30541 tctatagagg gtatgaatgt tcgatgattc aagaaaggta gaagaaaagg gggaattttt
    30601 atgtggcgat tattattttt ggtggtatgc ggtatggcat tgttctatgt gtatcgtgtc
    30661 tatctctatc cacgaatgga acgatggaca agttggtata gagatccttt aaaacaacga
    30721 aaaggattga agcggtatcg ctatgaatta cttgttgaat tttcagatgg gcgacaagca
    30781 cagtattatt ttttttgcaa actatccaaa atacaacacc agtgaactat tgattcaatt
    30841 attcaaaaat aatccatatg ttgggttaga agaaaacgga cgagagttct tttatcgttc
    30901 agataatatt agtgcggcgt ctatgaacaa aacacgaatt gcataaaaaa ttggaggaat
    30961 aatcatgata aaaacaacat tagcagtcgt aacaacagta gggattacac tattttcgtt
    31021 tcagttagga acgttattgg taaatgcaga tgaaactgta gaaacgccta agtcagaaat
    31081 tgtagacaca ttgccagggg aagagttacc agatgttgag acagaactaa gtggaggagc
    31141 aagtgaaact ccagaacaat ctcaaccaga acaacctgaa aaagaactaa tagagtcgga
    31201 accagagttt cctgagtcaa ctttaccgac tgatccaatc gaagaagaag gtgtgcatgg
    31261 ctcaacaaca acggattcaa gtacagtcac aactccagaa caatcaggaa caactagttc
    31321 ttctgaaaag actaccgatt cgtcaagtac agaacagcca acgatgtcgt ctaccagtga
    31381 agaatcaaaa gaagaaccta gtcaacctgc tgaaaaggca agcgaaaaat cagaagcaat
    31441 tccaaaagaa aataaacctg ttgttccagc aaaagaagtc acagtatcgg taaccccaag
    31501 cggtgaaatt acgacgaata cttctcaagg aaccagtgta ccgattgtca ctagtaatgt
    31561 agaggaattg acccatattc caacaccaac caccccatta aaagtagaaa gtggtcaaac
    31621 aattgttggt gtaaaagatg gaattccatt agtccaagat agtgaaggga atttggtaaa
    31681 ggatttgtcg attcctgtaa agaaacttcc aagtggcaat attgaagtca aaacagcaga
    31741 tggaaaaaca aaagtcttac caaagactgg tgaagaaata catgttgtac tttctgttct
    31801 aggaggagtg ttgacaagta ttgctggatt tgttggatat aaaagaaaga gaaaaggaat
    31861 agaaaatgag ggtaattgaa tggactattt ttcagaaaaa caagcttatc gttgtcaatc
    31921 gtacgtacta gtaaaaacat actagaagga gtagcttatg aaaaaacgac aactacggcg
    31981 gaaagaaaaa gtttatacag agaaagtacc gaagcaaaag aaaatccatt caaaaaaagc
    32041 agtgaaatgg atttggtgtt tggttttatt tctttgtttt tcagggggct tggcattttt
    32101 tcaatccatg aaaacgaagg ttcaagtgtc gtttttgaaa gatgaaatcc aacaattaca
    32161 aaagaaagaa gtacaaagtc ggtcagaatt aacgtacaca cctgaaattg aatcctttat
    32221 gaatcgtttt gttgctttgt atatgaatac attcgataat catgaacaag aacaacaacg
    32281 tcaagaaaca ttaaaaaaag agttttatgc gcaaaacttt attcaagaac caggaagtgt
    32341 ttcaggtgtt cgaaaattaa ctgctgcttc gtttattgga ttaaaagaaa ttgaaaccat
    32401 tccaacagct agttataaag taacttatga tattcgctcg atgaatccag agagtaagaa
    32461 agaaacaagt gtgacaatca cacaagtact caatcttccc ttttctattt caaacaaggg
    32521 ctgtcgtatt attgcttatc cttattttac ggcactacca aagaatacag aaaaacaacg
    32581 aatgattact tatgatgaca cattatacga aacggttgat gcgtcggaag cgaaaggtgt
    32641 tgaaggattt gtggaagatt tcttagataa gtatgcgaaa aacaatgcaa aagatatgtc
    32701 atacatgatg aaagaacctg aaggactcaa tcagacagct aaaattgatc aagtagagat
    32761 gaaagtattg cgttataaag aaacgaaaaa accttttatt gtaaaagcaa cggtcgtctt
    32821 tcgagatggt gatacgtcac ttttacaaaa agaacaaatg acgcttgtta ttacgaaaaa
    32881 aacaaatcag tattatgttg aaaagctaac acataattgg ttggatacga aaggagaaga
    32941 acggaaatga cactgaaggg aattgtagat tttatattaa cgcaaggtgg ctacttttta
    33001 ataatggtgg ttgtttatct ggggttacga aattttatga aatcaaaagt aggaaatgca
    33061 ttaatatcta ttgcagcagg tgcagcagtc tatttctttt taaataatcc aacacgtgta
    33121 tttgatgcgt ttggtaaaat cattgagaaa ttatttatga attaggagaa tagaaacatg
    33181 gattgggaac cttatgatta ttcaattgtt tatgcggaac cttatcgcgt acaacagatt
    33241 acaagcaatt ggcgtcttcc atttccagtt gcattagtca atgcagcaac ctttttgttg
    33301 gtcgcaatct ttgaatattt atttttacgt tcgtttatta gttgggttat ttcttttttt
    33361 ccaccagcag gagttgtttt ttatataggg ctaccctatt tatgtgtgtt agcactggca
    33421 aaactaaaac ctgatggaaa aaaagtgcat ttttatttat gggattggtt ttgttatgta
    33481 gtgcgtatga tgaatggtga ttgtgtgtat tataactggg acaaggtatc aaacaatgca
    33541 ttggaagaat tactttttgt gaataagtat gtttatttag aggattagaa aaagggacaa
    33601 tcaactggtt gttccttttt ttattgggag gtgaaactta ttggtgcttg taaaagaacc
    33661 agtaaaagca tttaagaaaa attgtttgtt aaatggcgag gattgtatat gggcctacta
    33721 tcatgttttt ccacgaacaa tttcaaaaaa cacaccaaaa aagacacagg aaataaaaga
    33781 aatgatggct cgtttactgt tgcttttagc agattatgaa gatgtggagt tggtgatgta
    33841 tccacgaaca atgaatttac gaacaaagtt tgacacctta ggtaagggat tttcacctga
    33901 tagtcaagcc attggtgcgt attatgcaaa agaaacagtg acattgttag aacgagaagg
    33961 gcaagtctat cgtccttcat ttattttagg aatcaaagtc aaagaaaaag caacggtaga
    34021 gagctttcat gatgtattaa atctaatctg gttagatgta gcaggaagtt tagcacgctt
    34081 atttggatta gaagtagatg atttggaaac tttgttttct gaaagtgatc cttatgtaaa
    34141 agaagtgaca caattacttt atagcttttc tattcgtccc ttagtaaaaa aagagctgtt
    34201 gtatctcaat cgtctgcaat atattcgagg aatggctcat aaagtagaag aacaaaataa
    34261 ttgggaagta ctgaaagaca atcaaggaac gtatgtacgt ataccgaagg aacataaagg
    34321 ccttcttcaa ttagaagatg aattggggac tagttattta gcgttgttac ctattggaga
    34381 aacacctgaa aatatgagaa gttgtgatct atttcaattt gcacaggatc tttctttccc
    34441 agcagaatta cgaatgaaaa ttcactatcc acgcttgaaa ggacagggag gtattggttt
    34501 aaaactgaat tggctatcgg aacggttcaa aacagaacat aaggaattgg ttcaaaatga
    34561 tgaagaacct agtgaacgct tattaactgt tcggggatta gtaaaacatt taaagcaacg
    34621 agtagataat gggcaacctg tcgtcgattg gatcgcatgt tttattgtat acggtcaaac
    34681 ggttgaagaa gttcgtcaaa atagtgcaac gattattcaa cagcttcata cgattatcca
    34741 tatccaccgt gcaaaaaatg atcaacgaga attgttctat aaatgtttat taggacaacc
    34801 attgggacgg cagttacatt ggaagcaccg aacaactgta gaagcagttg gtgaatggtt
    34861 gttttcagca gtatcagagt taggtatgga atctggctgg tatattggtc gtcaagatac
    34921 attaggggac ggtggttcta gaggagagaa aacaccgtta gaagaattga tctacgcgtc
    34981 aaatcgcttt gtctttttaa atcttttagc cattgcggaa ggaatcaaag gtgcgttgtt
    35041 tgatgcgcct catgtagcag taactggaaa aactggtaaa gggaaaacat ttttagttgg
    35101 cttgctcttt ttgtatagtt cgtttatgaa tgtacaaagt ttatttgtag atcccaaagg
    35161 agaaaagcgc aaatggttca cgaaactcat tcaagacccg tattatcaga aacattaccc
    35221 tttgtttgtg gaacatttgc aaaaaatgag ttttgtcacg ttagatgcta caaatccccg
    35281 taattatggc gtattagatc caattgtttg tttatcccga gtagatgcga aacaagtagc
    35341 ccaggatatg attgatgagt taagtccgtt aggtaccaat caccttttaa aaggggcggt
    35401 attaaagggg atccaagaag ttttagttca aaaagaagcc gggcaacaag ttggcttgat
    35461 gcatgtggtc gagtatttag aacaaatgga acaaaaagag gttcgagaat atggtgagtt
    35521 tttacgcttg acggtagaag attcgatttt acgtttagga ttttcttatg gagaaaaccc
    35581 aggattagac tttaatgcca aaacaacgat tttagagatc caagatttga aattaccaaa
    35641 tgacaatgtc cgaccagaac tatatacaga tgcggatcga aagtctttgt gtttgatgat
    35701 tagtttaggt cgtttttgtg aaatgtttgg aaaacgagat agtagtaaga aaacagcgat
    35761 ttattttaca gaagcatggg tatttaataa ttcgaacgca ggacgtagta ttattgcagc
    35821 aatggcgcgt gtggggcgtt cacagatgaa tcaattagtg ttagatacac aatttattgg
    35881 cgacttaggt tcagataaag aaaaagggaa ttttggtgtt gtcttcgctt ttgatgaaga
    35941 cagtgagcga gaaaagattt tgactcattt acgtttggaa gtaaatgagg cgaacttatc
    36001 tgaaatgagc catatgatta aaggacagtg ttggatgtta gatccctatg gacgtgtagg
    36061 aaaactcaat gtacacagtt tgtttgaaga aatgaccgct gcattacaaa caacggaaaa
    36121 aacaagtcac tctcaagtag aagaacagga tacgttaatc atgcaataga aatgaggtga
    36181 aacaatggct tatataaagc ataaggaaaa gaaatggtgc gttttgtttt ttttaacgat
    36241 tggattcgga ctgctttttg ttggtggatt ggtgtatgcg gcagaaaagc aggtggatca
    36301 atcgatctat gagattggga aatttcatat tgataccttt tcttcttatt tatctgatag
    36361 tgaattttca ttaggcaaaa tgttatttgg ggcaaatggc acagattttc taaaaatgat
    36421 gacgaatatg ttctttagtt taagtaaatt gatttggcaa ggccttgatt ttgtcattga
    36481 gaagctctat gaaggagctg caatgaacga gttaattcac cagtttttca ctttttcgga
    36541 tactatttat tcaaaaatat acgatcctat tggtgcgggg attgtcgtct tgtatgtgtt
    36601 atatctattt actcttcgta tatttagagg gaaacattac gcaaaaatgg tgtttattcg
    36661 atttctcttc gttgtcattt tttcaatggt ttggtttggc tatggtagta acgtacctag
    36721 taaaggagaa gcttttacca aagggttaaa tgatttgtcc gtggaggtag aaggctttat
    36781 tttttctgct acaaatagtc tagatgaatt ggcgacaaaa gatgtcaatg gtgttttagt
    36841 gccaacgaca caaaaggaag ccattcaaaa agtgcgccaa ctttattata aagcggctgt
    36901 tgttgatccg tatctattgt taaattatgg gacaacaaat ttagaagaat tgtatcaagc
    36961 agagattgaa ccacaagaat ttctagccaa ggaagcgtca agtgaagaat tatctgatat
    37021 tgagacaaaa gtgaccactg ctgcaggaga agatagaggg aaaaagaagg cttatcgtgt
    37081 ctatattcaa ccaactaaag ctgtttataa agcagtcgtt ggttctatgg tgccattgct
    37141 taatctaagt attggattac cacttgtagt gattggcatg attcgtttta tctttcaaat
    37201 gttggtgttg tgtttattac ttgtgattcc tttttcatta ctcgcttctt ttgttccagt
    37261 atttgattat atgctcatga ataccttaaa gtcctttttg ggcttgatgt ttcaaaaatc
    37321 tttttacagt gtgattattt tggtggcatt tttgatctat aacatagtag acgatttaat
    37381 tccaatgaat actgtttttg gttttttagg aaatatgttt gtcaaagcac tcctatcaat
    37441 tttactcatt gtaaaacgga aagatttatt aaagaaacta gggttaggag ctgcagatca
    37501 aactataaac aacacgaaaa acatagcaag tcattctttg tctcaaatga aacaactgcc
    37561 tggtcaacta tcaactgctg gacaaaattt ggcaatcaaa ggtgcaggat tagcagggaa
    37621 tgtgtatcga gcaacgaatc cactcgcttc tgcagcgata catggatttc aaaagtttca
    37681 acaaaaacgt acaccacaag aatcggtaaa agggaggcaa gtgattaatc cctttgaact
    37741 aggtgggcgt tcaaatcaag ccttgcctta tagtggtcgt catgttcgtt atcatggggt
    37801 agagcctgaa atgtcttcta ccaaatcgaa aacagagtgg aattctggtc tttcaaatgg
    37861 tttgattcca aattcgtcca tgaattcagt aagaccaaaa caacaggtac aatcgttggc
    37921 acaatcgagt aagcaacaag ggaataatca aagaaaacca ttgttaaata aaggtatcgc
    37981 ttttgttggt caatcgattc cgttgcctgc tggaaaagta cccgtgccac aattaacagc
    38041 tgctggttct ctttcccaac agattcgcca agtgaaaaat caggcgaaaa atgggagtta
    38101 tccagcaatg cagcagttat tgaatcaata ctctgttcct aagtcgtcgg tagccactct
    38161 atcaggaaat cgttctgtgc aacaactggg acaacgttcg tctgaaagta aaccgtcgat
    38221 ttccgcacca aaatcgttac aacctgcatt ttcttcttta gataagtcaa cagatcaatc
    38281 gaaaaaaata gaaaaggatc ctaaaacact gccgattttg gatgcaaaac taggaaccga
    38341 ttttacttta ccaaaaggac gaacagtcca aaaaccattg tcgaagaaag gatgaggaag
    38401 aaatgaagaa agggtgtcta ggttgtcttc ttttctttat tcttcccttt tttctattgc
    38461 cattcttttt ttcaggagaa ctttccgagg atggaggaga ggctacaggt ctagctgtcg
    38521 cttttaatgg ggaatatacc gaaggattgc cgattttttc agagattaga ggacgtggtt
    38581 cgatcacaga tgaacatgcg caagcagcag ttggtgcggc ggtaaagtat cagttgttgc
    38641 cgagtgtgat tctttctcaa ttgggttatg aatcggctta tggacagtca caatctggaa
    38701 gaaacgatac gaactttttt gggattacct ggtttccagg ctgtccctat ccacaaggtt
    38761 ctgcacgggg gattggcggt tctgaaggcg gcttctatat gaagtttccg aatctaaaag
    38821 cttgttatga ctattacggc tttatggtgg cgagtcaaag caactttaat caatgtgtgg
    38881 gcaataaaga tcctgggagt tgtttattaa ttctaggtcg tggaggctat gcggcggcag
    38941 gtattaccga aggcagtccg tattaccaag gagcgatgag tatcataaag tcttataact
    39001 taacagaata tgatactttt gcgatcaaac attggaatag cctaccaaca aaaaaatcgt
    39061 cacttgttgc agggaaaagt aaaggtaatg cgaaagtttt agatcggatc attggacgat
    39121 cggtaaataa tggagagtgt tatggtggca cggcgtggta tgtatcccaa ctaggtggcc
    39181 cacagttaat gggttctgga cacatgtttg cgatgttaat aggggaagat tatgattgga
    39241 cagcctatgg ttggcaagtg atcatgaacc caaaaccaag tgatttacaa gcaggagatg
    39301 tggtgaattg gcaagcaggt ggtgcattgt ctcctggaat ctatggtcac accggaatta
    39361 taacagatgt ttcatatggc ggacaacatt tctctagtgt ggaacagaat gccgagcaag
    39421 gacggattat ggctcggtat caacgcacgt acaatcaaac aaaaattcgt agtctggttc
    39481 gtaagatgaa ataagaaagg cagtgactag aaacgtgaaa aaagggataa ttttagtatt
    39541 tagttggtta tgtttgatag gtcttactgg ttgtcattgg ttttcttctg aaaaacaaaa
    39601 aacacaattg agaacgacag aagaaacaat cgaagcagta aaagaaacaa gtggaactct
    39661 tcaaagtact gaaagtaaca gtcaaattgg ttcattagaa gacgagacag cgcaacaaat
    39721 gcaaatagat gaaatgacaa aaaatttctt aaaagatttt gctagaaaat ggagtaattt
    39781 cacagatgtt tataaacgca atcaagctgt gagggattat atgacagatc gttgtattaa
    39841 agacaatagt attgatgtag acccgcatgt acaaatgaaa acaactggga aactttacca
    39901 ggtgacacaa gatatagaga atccggatca atatttgtta tttggagaag aaacaatgaa
    39961 taaggttact caatttgtat tacttcaatt gaaagtagat caagaagctc aaaagattga
    40021 tgcgatcaag atttattatg tacggagtgc gtattaaaat aaaaaatgaa cactcatatt
    40081 tattatacac cgaagaaata gaaaaaaaca gactgttttt ttaagaaaaa ctatgtcatt
    40141 ctttacttga aaaaagttgt tataggacag aaaggataaa acaaaaacgg aaaaaatatg
    40201 tcttatgaat aactataaga actattctat tttttaggag atggatacga gatgaaaaaa
    40261 ctaattggct atagtgtgat gtgtattgga tttgttggtg tgggattgtt tgtatcgact
    40321 gaatctgctt ctgcggctga atggtataat cctatctggt ataatccgaa cattgaatgt
    40381 caaaaaggct atccgtatgg accgacaaat ctgaaaaatg gaaaatgtag agtaaattgg
    40441 aatggcgttg tagatagtgt aattactggt tcaatggact ctgctctaca tggctttacg
    40501 ggtcactaga aggaggaaga aaatgtctga agagaaaaaa gaaatcgttg aatcattagt
    40561 agctttaaaa gattctcttt ctaagattga atatgttgat aggcaagaaa ttcaaaaact
    40621 aattgatgat actattattg aaatccaaga tgcgcgttgt gaaggtataa aaatttctgt
    40681 agctttatct aaagtaatag aaaaaatgaa ccgttcatta gcgtttaatg gattgaagct
    40741 agatagacaa actagtctga tatgggatca tttaaaagac ttgtatgaca agtctaaaag
    40801 aagtgaacgg actgctgtat caatcttaaa aggcctttgg ggaatgaatt cataaacaag
    40861 atatttaaaa agtaacttat tttttatgga ggagaaatta tgaaaagaat aattaaaata
    40921 tttggtgttt taggtgtttt cttattaggt ttaagtactt ttgttagtac tggaacaagt
    40981 gtgtatgcca cagaaaaagt gaatacagtt gatttagaaa taaagaataa attagtagat
    41041 gaattaactt tttattttga tacagttggt tttttagacg aacatggtgt ataccatata
    41101 aaggattttg gtgctattcg tgaacgaatc agtcaagata acgaacaagg aagagcagca
    41161 agagtctttt atgaacatat tattgttcct agaatggaga aagttgattg gaaacgatat
    41221 gctggatgtg tagccattaa ttctcttcct ggaggaggag tggcttgggc tataggtacg
    41281 cagggagatt ttattgaagc attaaaagct tttaattttg aaaaagctgc ggatttatta
    41341 ttaaaaattg cagaaaaaca attaggaaaa gctgcagcta aagagttagc taaggtaaat
    41401 attgtagcag gaattgcagc agcagctgct agctgtgcat tagagtaaaa gtttatgaaa
    41461 agtttagaac aagagcatat ggcttttaaa caagcaatgt ttaaagaaaa tatctattta
    41521 aaccataact atattcgtgt ttctaaggca tgttcaccag tattgaatat gcttggaggt
    41581 ggaaatggat tatatcattt actgtttgtc gatgtttgtt ggttaatttt tttaccagat
    41641 gaactagtta tagtcaatga aaaaataaca agtaaaaatg aagtgtttaa ttattc
//
LOCUS       DACECZ010000010        39857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.010, whole genome shotgun sequence.
ACCESSION   DACECZ010000010 DACECZ010000000
VERSION     DACECZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.010"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWO35_000578"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWO35_000578"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ0315656.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWO35_000579"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWO35_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ0315657.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWO35_000580"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWO35_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ0315658.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWO35_000581"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWO35_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ0315659.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWO35_000582"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWO35_000582"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ0315660.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWO35_000583"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWO35_000583"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ0315661.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IWO35_000584"
     CDS             complement(8053..8523)
                     /locus_tag="IWO35_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ0315662.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IWO35_000585"
     CDS             complement(8569..8994)
                     /locus_tag="IWO35_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ0315663.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IWO35_000586"
     CDS             complement(9061..9330)
                     /locus_tag="IWO35_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ0315664.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IWO35_000587"
     CDS             complement(9341..10936)
                     /locus_tag="IWO35_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0315665.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWO35_000588"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWO35_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ0315666.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IWO35_000589"
     CDS             complement(14489..15049)
                     /locus_tag="IWO35_000589"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ0315667.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IWO35_000590"
     CDS             15402..16376
                     /locus_tag="IWO35_000590"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0315668.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IWO35_000591"
     CDS             16546..17946
                     /locus_tag="IWO35_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ0315669.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVNLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IWO35_000592"
     CDS             complement(18024..18746)
                     /locus_tag="IWO35_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0315670.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IWO35_000593"
     CDS             complement(18825..19331)
                     /locus_tag="IWO35_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0315671.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IWO35_000594"
     CDS             19454..21253
                     /locus_tag="IWO35_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ0315672.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22859)
                     /locus_tag="IWO35_000595"
     CDS             complement(21321..22859)
                     /locus_tag="IWO35_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315673.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVTPAPAP
                     SGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWTVPQES
                     AGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQWFAPDF
                     AVSM"
     gene            complement(23114..23623)
                     /gene="mreD"
                     /locus_tag="IWO35_000596"
     CDS             complement(23114..23623)
                     /gene="mreD"
                     /locus_tag="IWO35_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ0315674.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23627..24481)
                     /gene="mreC"
                     /locus_tag="IWO35_000597"
     CDS             complement(23627..24481)
                     /gene="mreC"
                     /locus_tag="IWO35_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ0315675.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24636..25274)
                     /locus_tag="IWO35_000598"
     CDS             complement(24636..25274)
                     /locus_tag="IWO35_000598"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ0315676.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25275..25925)
                     /locus_tag="IWO35_000599"
     CDS             complement(25275..25925)
                     /locus_tag="IWO35_000599"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ0315677.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25938..26837)
                     /gene="rsgA"
                     /locus_tag="IWO35_000600"
     CDS             complement(25938..26837)
                     /gene="rsgA"
                     /locus_tag="IWO35_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ0315678.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27002..29071)
                     /gene="pknB"
                     /locus_tag="IWO35_000601"
     CDS             complement(27002..29071)
                     /gene="pknB"
                     /locus_tag="IWO35_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ0315679.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29068..29808)
                     /locus_tag="IWO35_000602"
     CDS             complement(29068..29808)
                     /locus_tag="IWO35_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ0315680.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29821..31179)
                     /gene="rsmB"
                     /locus_tag="IWO35_000603"
     CDS             complement(29821..31179)
                     /gene="rsmB"
                     /locus_tag="IWO35_000603"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ0315681.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31179..32117)
                     /locus_tag="IWO35_000604"
     CDS             complement(31179..32117)
                     /locus_tag="IWO35_000604"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ0315682.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32110..32601)
                     /gene="def"
                     /locus_tag="IWO35_000605"
     CDS             complement(32110..32601)
                     /gene="def"
                     /locus_tag="IWO35_000605"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0315683.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32616..35024)
                     /gene="priA"
                     /locus_tag="IWO35_000606"
     CDS             complement(32616..35024)
                     /gene="priA"
                     /locus_tag="IWO35_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ0315684.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35277..35582)
                     /locus_tag="IWO35_000607"
     CDS             complement(35277..35582)
                     /locus_tag="IWO35_000607"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ0315685.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35587..36201)
                     /gene="gmk"
                     /locus_tag="IWO35_000608"
     CDS             complement(35587..36201)
                     /gene="gmk"
                     /locus_tag="IWO35_000608"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ0315686.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36688..36972)
                     /locus_tag="IWO35_000609"
     CDS             complement(36688..36972)
                     /locus_tag="IWO35_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315687.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(36990..37880)
                     /locus_tag="IWO35_000610"
     CDS             complement(36990..37880)
                     /locus_tag="IWO35_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ0315688.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37900..38118)
                     /locus_tag="IWO35_000611"
     CDS             complement(37900..38118)
                     /locus_tag="IWO35_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315689.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38088..39788)
                     /locus_tag="IWO35_000612"
     CDS             complement(38088..39788)
                     /locus_tag="IWO35_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ0315690.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
BASE COUNT        10768 a         8691 c         7241 g        13157 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaataa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtca atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcgctgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgattct tctgttgaag attcaggtgt tgttgattct
    21961 tctgttgctg aagattgtgt agctgatgat gattcttcag tcattgaaga ttgtgtagct
    22021 gatgattctt ctgttgctga gctttgtgca gtagatgaag cttgagtagc ttgtgcttca
    22081 gcctctgctt gttcgcgtgc ttctttttca gctttttctt gtgcagcttg ttgacgagct
    22141 tgttctgcta gacgttgttg ttcacgaata cgagcttgct ctgcttcagc ttctgctttt
    22201 tgacggttca agtcagcttt cttgtcttca gcagttgctt gttcagcagc taatgaagtt
    22261 ttcagtacgt tcaaatcagc ttgtttagat aaaagatcgc ctttttgaga ttccaaagca
    22321 gcttggtttt cagctaattc ttttagttta gcatcgtttt cagctttttt gtcttctaca
    22381 gcttttttat cttgtttttg ttgttccatc aagtcgttgt ttgcttttac catcgttgtc
    22441 attgcttgaa cgcgtccaat agcatctgct aatgaatcag cattcaatac agcatcgatg
    22501 tagtttgagc ttgtgttgct tacttgtgct tcgcgtgctt gtttttggat tgtatcttca
    22561 cgtttttcaa tacgttcttg taaatctgca atgtctttta ctaattgtgc agattcttga
    22621 cgcaatgtat cttgttttgc taataggtct tgtgcttgtg tgttgatttc agaaacttgg
    22681 ctttctaagg catcgatttg tgattgtgca tctgcttgtt gatttttcaa gtctgcaatc
    22741 ttttgatctt gctgttgtat ctgagaatca aaatcgtccg ccgcggcagc gatcggtgat
    22801 gctacggcag tcaaggtcat tgaacatacc attactgctg atattaaact ctttttcact
    22861 cttcattcct ccgactggct taatttaata aatatctaaa tatatttaag tctttcttaa
    22921 cgatataaaa attgtaccat agcagtgtgt caacgatata atagttatgt tacaaaaatg
    22981 tttcaaaaca acttttaaaa atgacaaaac tgttcgtttc agaaaatgat catgaaaaag
    23041 ccgcgttaag tctagcattt cttaagataa caacgattct ttttcgcttt cgacctaaaa
    23101 ataaaaaaag aaattattca tcactgaata atttcttaaa gggaaaaatg aataatacaa
    23161 atataatcat gttcaacagc agtgtaggtc ctaaaaacct tgtaataaaa tacgtgttat
    23221 ttactactgc taatttaaaa atcaactgga ccaccatcgt aaacaattca taacccgtga
    23281 caaagatgat catactgaaa aattcagtaa agatgttgac atggataacg tctttcatta
    23341 catacatcaa ccatacaatt aaagggagag ctactgcata gataccgata acgccaatat
    23401 agtaagcgtc atagatagcc ccgagaacaa tcgtggtaat cagtaaataa cgtttttcaa
    23461 acgccatact gcaacataat aatgccaata tcagaaagtg ggcatttgac atataggtgc
    23521 ctttcgacca ctcccctagc attcttgtta aatgaccatc tatcaacatc aaaaagaaca
    23581 aaacgattgg cagatagtac ttcattgttt ctttcttcaa catggactaa tcctccgcta
    23641 gccgttggac aatcgtcaca actgacacgt catacatttc tgcataaggt ttcacataaa
    23701 cttcccgatc cagcccataa ctatctggtt tcgttttaat aaccgtaccg atcggcaagt
    23761 tagctggaga attccctcca agaccggatg tctggacaac atccccttct ttgatatccg
    23821 tatcaccagt taattgggtc accactaagg catggagctt ttcatcatag tttttaagca
    23881 aaccaaacgc ttcgccatta gccgaagata cccgtactgg aaaatgattg gagctttcat
    23941 tagaggatga cagtaattcg attttagacg aagccgtatt gacctcgatc actcggccaa
    24001 tcaatccttt ttgcgacatg actgccatat ttgcttcgat accgtcttta gaacccttgt
    24061 ctacgatcag catgtcctgc caactgtcag gcgaacgagt aatgacattt gccgttactt
    24121 tttcgtaact agtcagcgtc tgattcaact caagttcttt tttcaacgct tcgatttctc
    24181 tctcgtaatt cttgttttgc tgtgaaacgt catcatagct atcgattttt tcttttagcc
    24241 gctcattttc ggcataagtt gaaaaaagat tatggacaga atctactcca tttcgtaccc
    24301 accgtactgg ggcagagatt gttttatcaa ccattgcaac actgtcatta acgatggatt
    24361 ggaagaaatt gggctttcct tcattcgctc gctgcgcagc ggtcacactt agtatcgtca
    24421 ccacgatgat aacaataacc aaggtaataa taatattttt attaggattg aacttcttca
    24481 caaagacacc tcgaattaat tagactcttg tgttcaattt tatcactaaa tgccgtgaaa
    24541 caaaaggtaa gttgctcctc ttaagctttt ttttagcaaa taatagattc tgtcattttt
    24601 agcctgaaag acaaattttt ttatttgttt atggatcaat ctttgctttg aataactgca
    24661 atcataccat ctgaaaaaga aaattctgcg gtatctgaaa gaaattcgtt acttgcataa
    24721 gatgtcggat attctacttc tttattttcc aatacatact tgctgccttt taatgtaaga
    24781 ttcgagacgg gagtcaagca gcaataagct aaatacttca tatctggtat ctgtcttact
    24841 atatgctctc caggcaaata ataagacaaa taattttgac aatctctgat aataagctga
    24901 tgggcaaagg gtttgaagcg aggttccata cctaaccaga gattggctaa aagatgatcc
    24961 aatcttccgc ccgtagctcc aatcaaagtg acttccgctt gcgggtaacg ttcaaatgtt
    25021 tttaagatac ctaactgtgt atctgtatcg tctttttctg caggggaacg ggtatactct
    25081 ttggcttttg caaatacttc cttctgctct tcgttcgaca atgaatcaaa atcacccact
    25141 gccagatcta ctgggtagcc tgcttgaagc aggtacagcg caccacggtc tatcccgaca
    25201 taggcatcgt atttggacag cagagactgc ggccagtttt gcggattacc tccagcagct
    25261 aataaaattt tcatttagtc taatgcctct tttaatcgtt cgatttgtcc gactggattt
    25321 tcggctccat agatatatga tcctgcaacg aagacatccg cccctgcatc tttacagatt
    25381 tttgctgttt ctggaacgat tccgccatct acttcgattg cataagtcca attgttctct
    25441 ctacgtagat cagcgagttc cgtaattttt tctactgttt cttcaataaa gctttgtccg
    25501 ccgaacccag gattgacagt catgactagt acttgatcag ccaaaggcaa aacatgtttg
    25561 atcatggaga ctggtgttcc tggattgata actactccag ctttaactcc taagcttttt
    25621 gtcatttgaa gtgcccgatg aatatgagga gttgcttcga cgtggacagt aatgatatct
    25681 gctccagctt ttgcaaatgc tggaatgtag ttttctggat caacgatcat cagatggacg
    25741 tctaacggta atttagtaac aggacggatt gcctgaacaa tatttggccc taatgtgata
    25801 tttgggacaa atcggccatc cattacatct acgtggatgt aatctgcgcc tcctttttcc
    25861 actaactcaa tgtctctttg taaattggca aaatctgcac tcaaaatgga tggtgctatc
    25921 ttcattcttg tttcctccta ttttcgttta ccttcttttt ttgcatacat tggttttctt
    25981 ttttcgattt cttgtaagaa ttgcaaataa ttgtcgtaac gtgtctgtgc gataccacct
    26041 gtttctactc tatgttttac ttcgcagcca ggttctttag catgcatgca ctctctaaat
    26101 ttgcaatgga cagaagcttc tacaaattca ggaaattgtc tcggcagttc tcctgcttca
    26161 attgtcaaaa agtcaatcga gctaaatcct ggtgtatctg ctactaaccc gccatagata
    26221 ggaatcagtt ccacatgacg tgttgtatgc cgtcctcttc caagtgcatc agaaatctct
    26281 cctgttgcta aattcagttc cggtgatatt tgattcagta gcgtcgattt tcctgctcca
    26341 gactgaccca taaagaccga tagccgttct gggaagtaac ggatcagttc tgtcgtatca
    26401 cccgtatgat tgggtacaat caccggatag cctattttag tataggtttg cttgatctct
    26461 tcgatttttt ctgggtcgtc tgacaagtcg gtctttgtca aaaaaataat cggttcgatc
    26521 gactcgtatt ccaaggtaac tagaaagcgg tcaagtagat tataagaaaa tgcaggctct
    26581 acgatacttg tgacaatgat tccaagatca acatttgtga ctggtgggcg aactaattga
    26641 ttttttctcg gcaagatttc cagaagatag ccgtctgttt gattttcact ctcaaagatc
    26701 acctgatctc cgacaagcgg cgtgattttc cgattccgga aatttcctct tcctcttgtt
    26761 tgataggtct ctccgtttgc gtatacatag taaaaaccgc tgattgcttt tctgatttgt
    26821 cctttgataa gtgccacctc cgtttttgtt tattttacca tacgaagagg aaagtaggtg
    26881 ttattccatg caaacttctc aaagtatatg gcttgaaaat tgatcaaaag agatagaaac
    26941 acaaaaaaga gaatgcaaca aatcaatgtc gcattccctt ctgatatatt ttattcagca
    27001 cttattttga actttctgag gtgctagctg atgaacttgt ggagctggaa gattcttctt
    27061 ttgaagagtt gcttgtgcta ctggagctac ttgagctgct agaactgtct gaagagtctt
    27121 tttcagaact agaagacgct ggatctggtc ctttagagta aattacgttc acaaccattc
    27181 ctttggtaat tgtcgttcct gcccctggta ctgtacgaat cactttatct ttatccaccg
    27241 tgtttgaata ttcggaagtc tcatgcccga tatattctgc accgacacta gctaaataac
    27301 tttgtgcttc gctttttgta tagccggaga tatcattcaa tttaggctct gttccttggc
    27361 ttacgacaaa attgatgtaa gtttcttctg ctacaacttc acccgaggct ggatcctgac
    27421 tgatgatcga acctgatggt acagtgtcgc tatagtcata agtagcacta atattattga
    27481 tattgaatcc ttgtgtaaca agccaattac gaacagtttc gtagttttct cctacatgac
    27541 tactcaaagt cactgtttct ggtcctttgc tgactgtgaa agtaatcgtg tctgttttag
    27601 gatcgacttc tgtatcttct gatggagatt gcgaaatgat cattccgctt tctacctctg
    27661 aactgaattc ctcttctttt tgaatcaagt cagcagaaaa ccctaattct ttcaaacttg
    27721 cttcggcatc ttcataactt ttaccactgt aatcatctaa tttgattttt ttcgtgccgc
    27781 tactaatata cagagtgact tcgcgttttt gcttgactgt cgttcccgct gccgggtctg
    27841 tcttcactac attaccttct tcgattttgt catctgcaat ttcttctgtt tttgttgcta
    27901 cttctaattt cgcttttgcg agtgtttctc tagcagaagc ttcagaaaga tcagaaacat
    27961 caggaacaac aacttctcct cgcccgccag aagcaaagaa tacagctgtt ccgattccta
    28021 aaacagcaag aatagctaat aggatcagcc accatttttt ctgtcctttc tttttaggat
    28081 caggcagaac ttcttcctcc tctggtaagg tttcgttttc tggcttgtct tttggcagag
    28141 gcatagattt gaatgaatca ggcattggtg catcttccgt aaggggtgtg ataacttttg
    28201 tttcattatc catcacatga ggctgccatt tttcttcgtt tgcacgagct ggtgataata
    28261 ctgtagaaag atcatcagac atttcctcgg ctgacttata acggtctgca ggttctttag
    28321 ccgttgcccg taaaatcacg ttttccagtg cttgtggcac atttggatca agtgctttta
    28381 tagatggaag atcatcttga aaatgtttta aagcaattgt tacagcagat tcaccatcaa
    28441 acggtacgct acccgtcaac atctcataaa ggatgatccc taaagcataa atatctgact
    28501 gcttcgttgc catacttcct ctggcctgct caggggacag ataatgaaca gatcctagca
    28561 tcgtattcgt ctgcgtgatc gatgtttcag ataaagcgat agcaatcccg aaatccgtga
    28621 tttttacaac accttcatta tcgatcagta cgttttgggg tttcaaatct cggtggatga
    28681 tttgatggtt atgagctaaa gaaatagcag aaaggatctg ctgcataata tttactgctg
    28741 tttcataagg aacaggataa tgtgtttgga tatatcgttt caagtccatt cctttgacgt
    28801 attccatcac cagatactgc atgttgtctt cttcaccaac atcgtaaaca ctaacaatgt
    28861 ttggatgaac gagttctgtt gctgctaaag cttctctctg aaatcgtcga atcgcagtct
    28921 ggtcattttg aaagtcaaat cgtaagactt taactgctac atcacgatcc aagattaaat
    28981 cgtgcgccaa aaatacattt gccataccac cgctaccgat atttccagtg atttgataac
    29041 ggccatttaa ttttttgccc aactcgatca tacactgtcc tccttatatt cgataagaag
    29101 aacggtgata ttatccgctc cgcctgcttc attggcacga tcaatcagct cagttacttt
    29161 atgagacaat gggccttctt gattgatgat cgttcctatt tcttcttcag acagcatgtt
    29221 cgtcaagcca tctgaacata acagcagtcg atcttttagt tgccaaagat gagtagaaac
    29281 atctacttcg actgttccag gcatgccgac agaacgtgtt aaaatatttt tccttggatg
    29341 gttcactgcc atttcttcgc tgatttctcc tgatttgact agttcattga ctaaagaatg
    29401 atcctccgtc aattgcatga ttttatcttc acgaatcaaa taagctctgc tatccccaac
    29461 attggcaatc gtgaaccagt catctgacag agcggccgct acgattgttg ttcccatacc
    29521 gttatattcc ggctgttctt gtccacgctg ataaatcagc atattttctt cttgaatcga
    29581 ttgaatgaac cattgggcga ttttttcatc gtcaacagct tcttgttttt cccaagcttc
    29641 gcctagattc gttaccgcca tctgactggc aatgtctccg gcacgatgac cacccatccc
    29701 gtctgctaat acagcaagct tgagacctga acggctggta aatacactgg catagtcttg
    29761 atttgtgtta cgtcgtcttc ctacatctga ttgatattca atctgcattt tttcacctca
    29821 ttactttttg cgcaaacaac aaatgaaaaa tccatctgtg taaaactgat gcggatacaa
    29881 agtcaacatt ttctcttcga ttgatgattg tagcatagga ttggtcaaaa catcaatcct
    29941 ctcaaaatca gggtgtcttt ccaagaaagc agaaacaacg tcttggtttt cttcttttaa
    30001 gatcgtacaa gtgctataag tcagaatacc ggaagacttt aaagttggtg cgcagctttc
    30061 taaaatttcc aattggattt ttggtaaatt cgctaattca tccggctgtt ttttataacg
    30121 aatatctggt tttcgccgaa gcagcccaag acctgaacaa ggcgcgtcta ctaaaatccg
    30181 atcaaactgc tctgcttcga actgtttact gacttctcta gcatccatct tttgagcgta
    30241 gacagcagaa gagacaccta gacgtgctgc attatcttca atgagtttga ctttgtgttc
    30301 gtgtacatct aaagcgacca cttctccacc gcatgacgga tctaaaaaag aagcaatatg
    30361 cgtcgtctta cctccgggtg ctgcacaggc atctaagaca tgatgttcag gctcgatttg
    30421 catagacggc gctacaagca tcgaactttc atcttggatc gtcatgacgc catcgtggaa
    30481 caatgagcta gaagctaaat gccctttttt cgctactata ccgtatggac tgacttggct
    30541 tttttcagct gtgatctctt cttcttgcaa aacagtgatg gcctgatctc tggaaagtcg
    30601 gcgtgtatct actcgggcac tagcatggct aggctgatat agcgataaac cgagcttttc
    30661 agtttcttcg tatccgattt gatccaccag tcgtttcgtc atccatagag gcaggctgat
    30721 tttgacagaa agtctttcga taggatcagc aatgccttgc agatctggtg ctccttgacg
    30781 ctgataattc ctcaagacac catttacaaa tttccctgtc ccaggatttc cttttacttt
    30841 tgcgatttcg accgcctcgt ttaaaatagc atgtgcaggg actttatcta aaaattctaa
    30901 ttggtataca gaaagataaa gaagcatctt gacccagtca tccacttttt ttgctttagc
    30961 gatcaatggt gcaacgtaaa attccaatag tcgctgatgg ctaatcgtac catagatcaa
    31021 ttctgtaaat aaacccacat ctttttgatt tagctcgctt tgtttgatca tctcattcaa
    31081 caaaagattc gagtaggcgc cacccttgtt cactcgctct aatgtcagta atgccatata
    31141 tcgtactgat tttttcattt ttttagggat ttttttcatt atttcacctg ctgaccaatc
    31201 tgaacttgct gaccgcttcc gttaaggaaa tcatgtacgg cctgttttcc tttaccagct
    31261 ggctgtaatt cattgatggc taagacggtc tgtttaccgc aagcaatcca tagatttttt
    31321 ttgtctttcc ggatgatcgt accaggtgct ttgtctgtag tttctgccaa tacttcaacc
    31381 gataataatt tccagcgtat gccctcatag gtcgtgaatg caattggcca aggacgcatt
    31441 ccgcgtactt ggttgtcaat ttcttctgca gttttatgcc aatcgattgc ttcttgttcc
    31501 cgtgtgatgt ttggcgaaaa agttgcttct gactcgtctt gaggtactgg tttttgtcca
    31561 tccaaaatat tgggcaatgt ttctagcaat agctttcttc ctaaaaggct aagcttttca
    31621 aacatcgtcc ctacatcatc ttgtttagtg attggtatgc tttcttgcgc atagattccg
    31681 cctgcatcca tttttttgat catttccatg atcgttacac cagtttcttt ctccccattc
    31741 atgatggcat aatgaacagg cgcacctccc cgatatttag gaagtaaaga agcatggaca
    31801 ttgattgccc ctaattttgg tacttgcaat aacttttcag gcaagaattg gccaaatgcc
    31861 gcagtgacga tcacatcagg caccaattca atgatttctt ccatttctgg cgaaccggag
    31921 attttctctg gctgcagcac acggataccg tgcttcagag ccgctttttt taccggtgtc
    31981 ggcgtaatta ctcgtttccg cccaactgga cggtcgggct gtgttaccac tgcctggatc
    32041 tcgtaacctg attctatcag cccttcaaga atcggcacag aaaaagcagg tgtgcccata
    32101 aatactagct tagtcatcaa gatgttcctc catatattct tcaagttctt cttccgggat
    32161 ctgctgtatc attttctcaa tgaacaagat accgtctaag tgatcgattt catgctggaa
    32221 ggcacgggct aaatagccaa aagccgtcac ttctatctct tctccgtctc gatcataata
    32281 acgcacggtc acttcatctg ctcgcttgac cgttccatag acatgcggaa tactgagaca
    32341 accttctacg tcaaggcttt caccttttgc ttcgatgatt tctgggttaa tcagttcaaa
    32401 tttctcgcct tcatctactt cgatcacagc aattctcttg ttttgtccta cttgaggagc
    32461 agcgatccct attccgtcat tcgcaatcat tgtctcatat aaattgtcta ataaagcgat
    32521 tgtctcgtcc gtgattacat cgattggctg agcagttctt tttaatttat cattagggtg
    32581 gatcaatata ggatagcgca taatttggcc ctttcttaaa taaagtttaa tggttcgttg
    32641 tcaattgcca caaaaagacc ttgtcgctgt tctttctggc ttccattcaa aatttcatcc
    32701 aaaacatgag gaagattcgg ttcatgttta tatttgatga ttagttgata ataatatcgg
    32761 tttttcactc tcaaaatcat gctgggcgtt ggccctaata gcaagctttg agggcttaac
    32821 acttctttta gctgattggc aatctgaaag attttctttg ccgctacttg ttcttcagga
    32881 tggctggcgg ttattttcac agtaaagtaa taaggaggat agcctccacg atgccgtaca
    32941 tgcatttcct gctgataaaa ctgctcataa ttttgttttt tagccaattc tatggcgtaa
    33001 tgttgaggat tgaatgtttg gatcacgact tcgcctgctt tttccgctcg ccctgctcgt
    33061 cctgaaactt gggtaagcaa ttggaatgta tgctcactag aacgaaaatc cggtaaattc
    33121 agggaggtat cagcattcaa caccccaact aatgtcactt ctggaaagtc caaaccttta
    33181 gcaatcattt gtgtccctaa taatatatct gcttcttttg caccgaattt ttgtaaaatc
    33241 tgttcgtgtg ccccttttct tcgagtggta tctacatcca tacgcaaaat cctagcttgc
    33301 ggataaagtt ctcggagttc ctcttctact ttttgagtac ctgttccata gtagcggatt
    33361 ttattgccgc cacaattagg acagcgattg ggaataggtt cttcatgtcc gcaataatgg
    33421 cagcgcattg aacgtgtatc catatgcagt gtaagagaga tatcgcaatt aggacatgga
    33481 agaacaaatc cacagtccct acacatcaca aaagacgaat atcctcgacg attgagcagt
    33541 aggacagttt gttctttttt gtctaatcga ttttgtattt tttcctgcaa attagcagaa
    33601 aaagtagagg tccgatgatt ctgaaactct tcacgcatat ctacgatctc aatagctgga
    33661 agctgtgcag ctgctttggc tcggtggttt aactgcaata actgataaac gcctttttga
    33721 gctcgtgctc ttgattctaa agagggcgtc gcgcttccta gaactattgg gcaatgatga
    33781 tatttactgc gccaaattgc tagatctctc gcatgataac gcggtgtctc atcttgtttg
    33841 taacttgatt catgttcttc atcgataatg atcagcccga tatttttcag cggggcaaaa
    33901 atagcagatc gtgcgcctac tactacttgc gcttcttccc gctcaatctt gcgccattca
    33961 tcatattttt caccttggga aagtccgcta tgtaagaccg caactgattc tccgaagcgt
    34021 cctttgaatc gctcgaccat ttgcggagtc aatgcgattt caggcactaa cataatagcc
    34081 gttttatttt ctgacaaaac atcagcgatt gcctgaagat atacttctgt tttcccgctg
    34141 ccagtgattc cctccagcaa aaagacctga tctttttgct gctgacctgc tgtgatgatt
    34201 tgttcgacag catttttttg ttctgcattc agttccaatg ccgtcgtttg gtcaaatact
    34261 cggtctttat aagggtcgcg ataacgttct gattctataa actccagcca gcccttttct
    34321 tttccttgat tcaagacagc agtactgatt cctttatttt tgaaataagc aattggttct
    34381 tcttggctca gttcagataa acacaaaaga agttcttttt gtttcttggc attttgccgt
    34441 aattttgccc attcttcttc gatttgcttt tcagttaaca aagaacggac caaacgaatc
    34501 gtttttactt tatttcgtgt atggacttca tatttgatat ctactaattg ctcttggcgc
    34561 cagcgaatca attgagaaag gctgccttct tcttctgctt ccttccagga aatcacttct
    34621 cgctgatcaa acaatacctg aacttctggt gtgtctgcca aaggatagat caatttatca
    34681 tattctgctt tcatcacact cggaagcatc gtttgtagac aagtgatttt gaatgcgtaa
    34741 gtaatttttt tcatataatc agctaatgaa agtaattctt cattgaccac cggagcaagg
    34801 tctagcaaac gaataactgc cttcaaagat ggattagctg attcttctga tttttggata
    34861 gccataacaa atccttggac atgacgattg ccattaccaa aagggacttc tactcgcatc
    34921 ccgacttgca aagctgtttc catttcagag ggaaccaaat aagtgaatgg ctgatcggtc
    34981 tgcatagtag gtacgtccac aatgacttca gctgttaatg tcatggttcg tttcctcctt
    35041 tttttgtctt ttcttatcaa gcgacaaaaa gttccttgcc tattttacta gagatcgatg
    35101 gatttgtctt atcttctgcc aaatcctaac aaaaaattaa cagcagataa gaaaaaaaag
    35161 aactgtgtca aaaagttcag acacagcctt ttctaccaga caataagtgg cgaatgtttg
    35221 aaaaggaaac agctgggttt gcttttcttt ttagatgttc gctctatttt tctttattac
    35281 attttttgct cttcacggat acgtgattcc aattcgtgtt gttcacgctc tttttgtgct
    35341 tgacgttcct cttcttccat cttcaaacgt tcacgtttta attcaggatg tggatcattg
    35401 actacatttc ctgcatcgat ttcttctaaa gcttgaccga cgctttttac tgaatcaaaa
    35461 gaatctagag taggctgtgc acttgcatcc aattcatgcg cgcgtttact tgctaaaatc
    35521 actagagaat attttgaatt tacacgatct aatagtgagt caattgaagg ttttaacatc
    35581 ataagactac atctccttta acattttaat atatttaccg attacgcgat ctactcgaaa
    35641 atgttcactg gcaataatgt ttttgattcg ttcaactgct agcggcactt catcatttac
    35701 aacagcatag tcgtaaagag ccatcatttc gatttcttct tttgcaactt tcattcgttt
    35761 ttcgattact tcatctgcat ctgttcctcg gcctacgatc cgtgatttta attccgctaa
    35821 atctggcgga gtcaaaaaga tgaatacacc atcgggcact ttttctttga cttgttttgc
    35881 tccttggact tcaatctcca agaaaacatc ttttccttca tccaatgtct tgttgacata
    35941 tgacaaagga gttccataat aattcccgac atattctgcg tattccagca tctctccagc
    36001 ttcgatcatt gattcaaact cttctttgct tcggaagaaa taatcaacgc cttcgacctc
    36061 cccttcacgc attttacggg tagtcattga gatcgaatac tgaaaatcat tatcctcact
    36121 ctcaaaaatt gcttttcgta ctgtgccttt tcctacaccg gatggaccag acaacacgat
    36181 taataatcca cgctctgaca tgacgacgtc ctttcaacta gtctttttgt acttatcgtt
    36241 tattttgcac tttttttctg caactttcaa ggttatcttt aattatagct agaaattggt
    36301 aaaaaagcta ccctgtacca agaggtcgta aaaaagctgt cctgcatcaa gtaataagaa
    36361 cagccaaaca taaacagctt ctcatgtttt tcgtttggtt ggggttatta tcgcagatgc
    36421 aagcttttga acgccgtgta taagaggtcg tgaagaagcc gattagtttc gagtatgaag
    36481 aaaaattttg tgaaaatagt ttctcacatt tttgcataaa tttggcttaa taccgagaaa
    36541 atggtttttg aacatcactt attcaaaata aaaaaagatt gtgacacgaa ttttgtcaca
    36601 acctttaaat gtattcctag agcaaagaag ctaagcgaag aaatccaatt ctgtttattt
    36661 attgtatttc tttgttcagc atccttgcta aaagcccata ttggacaaag tattcactac
    36721 acccatgatc tcatcttttc gagaacgtgc tttttctgtc ggatcttcta cctcttctgt
    36781 gtcaattcga acaataaatt tccgattcat cgtcacgtaa gcaccgtcgt tgatcaaaat
    36841 aaacagttcg ttctcattat agtctgcaca ggcatctttc catacgtcaa agccgttttt
    36901 ttcagaaggg atatcccgtt taaccacagt ttttccactt aattcgtgga acgttacgtc
    36961 ataatatttc attatctcta cctctttcgt cattcaatat tttgtacttg ttctctgatt
    37021 ttttctaaga tcgtcttaga ctggacgact tgatttttaa tttcgattgc atttgatttg
    37081 gaaccgatcg tgttgatttc ccgattcatt tcttggatca ggaaatctaa ttcccgacct
    37141 acaggacatt gtgcttcgat aagttcatct agtttttgta catggatcgc cagacgatcc
    37201 agttcctcgt gaatatctcc gcgttccaac aagatggcta gctccgtcaa caaccgttgc
    37261 tcatcgactt tcgctccaag aaattcctcc agcttttttt gataccttat ttgaaattcc
    37321 ttttcataga tttctacaaa actatcgagt ttttttagta cttctttgaa ttcttcagta
    37381 ttctttttca acactaaagc taaagcttga ccttcttttt ctcgactcgc ctcaatagaa
    37441 tcaagtgcgc ttttcaatgt atctagagct aactgtgcca attcttccat gctctcttcc
    37501 ggtttttctt ctaccgttac gaagttttcg tccagcacga agcgttccgc catccgacta
    37561 attgacactt gattttgact accgtatcgt tgttgaattc cgtctgtcaa ttgagtgata
    37621 agtgaatcaa tcaattccca gttgatgacg atctgttttt cgtttttgcc aatataagct
    37681 agattcacaa atacttcgat tcgaccgcgg cttaactgct gtttaataag ctgacgaaaa
    37741 tcgttttcaa caaagttcag gattttagga cttcgtattt gaacatctag aaaacgctgg
    37801 ttgacacttt ttatttcaat atctaactga tacgcttttg tttcgcggat ggctttgcca
    37861 aaaccagtca tacttttcat tcgacagggt ccttcctcat tacatatttt ctattgcttt
    37921 ttgcagttgt tcaaactctt cgttcgtcaa tgtcaggcct ttccccattt tttcatggtc
    37981 tggcgcccaa tctcgaagat cgaattttgg cgggcgacca ttccagctga ctaggttcag
    38041 ttctttacgc caccccttgt tgttttctga caatatagcg atttcttcta cgatttcata
    38101 tgaaaattct tgtgccatgg ttgttcctcc ttgagataat ataaatatag gatcagtaat
    38161 gtgtcaactg gttgcttaag caattcatgc tgcaaacgat ccttgcgttt tccttgtagc
    38221 agataatcaa aataaacagt tttcgtgcgc tcattttgta ttttttgctg gtattttcga
    38281 taccttcgcc attcttcagt acttaattga ccaatttgtg ccaataaatc atccggcata
    38341 ctttttatat cttgttggca gatcatttga atcatcatgt ctacttgata gatttcattg
    38401 ggcagtaaaa aatgtttttt tgctaaaagt tctagcatcg ccaatccgca tgtctctggt
    38461 acgaccctgt tcttatataa attacgcagt ttttgccaaa attttatgct tttgaaaaaa
    38521 gataattcta actgaatttc ttttgttaag taagacaaaa atcctcgcca gtatgttctt
    38581 gcaggtttaa cagaatgaaa tgtataccag ttcccatagt aatagcctaa tcgttttttc
    38641 atttccagat agcctaattg taaatttaat tggttccttt ggctatcaaa aagcagaaag
    38701 ctgcctagtg tccacaatgt ttgacatttc caatggataa aaatgtctgg catacgaatt
    38761 tttttcgctg ggcctggccc ttgcacatct acgacaaatg cctctgttgc tcctttgtta
    38821 atcgcaatat caataggtat tttgttgcga tacccaccgt ctgcatattt attttttccg
    38881 atctttcgat aagccatagc gggataaaat gaagcagagg cgagtatcca atctggtatt
    38941 tcttctgttt ttgtctgttg gatatcagtc actacttccg ctagttctgg cagtttagta
    39001 gacaccgtat ataaacgtat tttgggatta tgtttgagta aggacaaatc caaattgtct
    39061 ttgaccatct tttccaatgg agcagaagaa accccgcctt caattagtgc tgttttcgtc
    39121 atttggcgtc tttctcgata gaagcgttca cgaaggtctt caactgcagc catttctggt
    39181 aacgcaagca cttgtcttgt cgacagcttt ttccataaat tcacagcttt tttcatatct
    39241 ccttgtaaaa tcaatgcgcc gttcaacgca cctaccgagg tacctgtgat aatctcaaag
    39301 ttgatatcat gttccttcag cgcctgccac acaccaatct gataagcacc gtgcgctcct
    39361 cctccaccta aaacaagtgc tgtatgatag accagctttt tatttaattg tgtggttcct
    39421 tgatagccgt ttttatgaaa aatacgcaac cattcttctg atagaggaga ttgaaataaa
    39481 aaacaacagt tttctttaat aaaaaaacga gcgcaagctt ccatataccg aaaaaatttt
    39541 ttccattcaa aagtttcacc ttcaatcaac agattattaa aaataacatc tcctctactt
    39601 ttagcaatag taaaatagac ggtatatttt ttcgatactg ctttaaaagc acattgcttt
    39661 tttgcagaac gccaaaaagc gtctctactt tcttgatgac tttgaaaaaa tggaaggggt
    39721 tgttttgctt gctggatagg cgaatacagc gacattttct gtgaaaagta aacttttttt
    39781 atctccatcg cgctctcctt tttcttcaag tataccagta cgtcataaaa aggtcatgag
    39841 tctcgtattc gactttg
//
LOCUS       DACECZ010000011        39161 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.011, whole genome shotgun sequence.
ACCESSION   DACECZ010000011 DACECZ010000000
VERSION     DACECZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.011"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(244..2625)
                     /locus_tag="IWO35_000613"
     CDS             complement(244..2625)
                     /locus_tag="IWO35_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0315691.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2615..3262)
                     /gene="recU"
                     /locus_tag="IWO35_000614"
     CDS             complement(2615..3262)
                     /gene="recU"
                     /locus_tag="IWO35_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ0315692.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3326..3865
                     /locus_tag="IWO35_000615"
     CDS             3326..3865
                     /locus_tag="IWO35_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ0315693.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3955..4374
                     /gene="gpsB"
                     /locus_tag="IWO35_000616"
     CDS             3955..4374
                     /gene="gpsB"
                     /locus_tag="IWO35_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ0315694.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4396..4763
                     /gene="rnpB"
                     /locus_tag="IWO35_000617"
     ncRNA           4396..4763
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWO35_000617"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4972..6138
                     /locus_tag="IWO35_000618"
     CDS             4972..6138
                     /locus_tag="IWO35_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ0315695.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6184..7680
                     /locus_tag="IWO35_000619"
     CDS             6184..7680
                     /locus_tag="IWO35_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ0315696.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7761..8444
                     /locus_tag="IWO35_000620"
     CDS             7761..8444
                     /locus_tag="IWO35_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0315697.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9089..10279
                     /locus_tag="IWO35_000621"
     CDS             9089..10279
                     /locus_tag="IWO35_000621"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ0315698.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10381..11334
                     /locus_tag="IWO35_000622"
     CDS             10381..11334
                     /locus_tag="IWO35_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ0315699.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11403..12752
                     /locus_tag="IWO35_000623"
     CDS             11403..12752
                     /locus_tag="IWO35_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0315700.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12935..13258)
                     /locus_tag="IWO35_000624"
     CDS             complement(12935..13258)
                     /locus_tag="IWO35_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315701.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13387..14550
                     /locus_tag="IWO35_000625"
     CDS             13387..14550
                     /locus_tag="IWO35_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315702.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14620..15969
                     /locus_tag="IWO35_000626"
     CDS             14620..15969
                     /locus_tag="IWO35_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0315703.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16014..16445)
                     /locus_tag="IWO35_000627"
     CDS             complement(16014..16445)
                     /locus_tag="IWO35_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0315704.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16833..19475
                     /gene="alaS"
                     /locus_tag="IWO35_000628"
     CDS             16833..19475
                     /gene="alaS"
                     /locus_tag="IWO35_000628"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ0315705.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19675..20271
                     /locus_tag="IWO35_000629"
     CDS             19675..20271
                     /locus_tag="IWO35_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0315706.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20393..21541
                     /locus_tag="IWO35_000630"
     CDS             20393..21541
                     /locus_tag="IWO35_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0315707.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21579..21992)
                     /locus_tag="IWO35_000631"
     CDS             complement(21579..21992)
                     /locus_tag="IWO35_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ0315708.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22071..22502
                     /locus_tag="IWO35_000632"
     CDS             22071..22502
                     /locus_tag="IWO35_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ0315709.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22601..22834)
                     /locus_tag="IWO35_000633"
     CDS             complement(22601..22834)
                     /locus_tag="IWO35_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ0315710.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23017..24687
                     /locus_tag="IWO35_000634"
     CDS             23017..24687
                     /locus_tag="IWO35_000634"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ0315711.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24834..25220
                     /locus_tag="IWO35_000635"
     CDS             24834..25220
                     /locus_tag="IWO35_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315712.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25207..25905
                     /locus_tag="IWO35_000636"
     CDS             25207..25905
                     /locus_tag="IWO35_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0315713.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25910..26374
                     /locus_tag="IWO35_000637"
     CDS             25910..26374
                     /locus_tag="IWO35_000637"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ0315714.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26371..27282
                     /locus_tag="IWO35_000638"
     CDS             26371..27282
                     /locus_tag="IWO35_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0315715.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27517..28056
                     /gene="pyrR"
                     /locus_tag="IWO35_000639"
     CDS             27517..28056
                     /gene="pyrR"
                     /locus_tag="IWO35_000639"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ0315716.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28070..29353
                     /locus_tag="IWO35_000640"
     CDS             28070..29353
                     /locus_tag="IWO35_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ0315717.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29366..30292
                     /locus_tag="IWO35_000641"
     CDS             29366..30292
                     /locus_tag="IWO35_000641"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ0315718.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30311..31591
                     /locus_tag="IWO35_000642"
     CDS             30311..31591
                     /locus_tag="IWO35_000642"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ0315719.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31596..32672
                     /gene="carA"
                     /locus_tag="IWO35_000643"
     CDS             31596..32672
                     /gene="carA"
                     /locus_tag="IWO35_000643"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ0315720.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32677..35862
                     /gene="carB"
                     /locus_tag="IWO35_000644"
     CDS             32677..35862
                     /gene="carB"
                     /locus_tag="IWO35_000644"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ0315721.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35916..36692
                     /locus_tag="IWO35_000645"
     CDS             35916..36692
                     /locus_tag="IWO35_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ0315722.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36689..37621
                     /locus_tag="IWO35_000646"
     CDS             36689..37621
                     /locus_tag="IWO35_000646"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ0315723.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37618..38334
                     /gene="pyrF"
                     /locus_tag="IWO35_000647"
     CDS             37618..38334
                     /gene="pyrF"
                     /locus_tag="IWO35_000647"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ0315724.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38341..38970
                     /locus_tag="IWO35_000648"
     CDS             38341..38970
                     /locus_tag="IWO35_000648"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ0315725.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            38958..>39161
                     /locus_tag="IWO35_000649"
     CDS             38958..>39161
                     /locus_tag="IWO35_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315726.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRII"
BASE COUNT        12614 a         6920 c         8530 g        11097 t
ORIGIN      
        1 aataaaagac tagtgtgtat ttagccgatt taattataaa caagtaaatg gataaagtat
       61 atgtaatgat aaaagaaaaa tggattttct aatttttaag ctaaatgtta gatcacacat
      121 gtatatgctg gatagtctta taaaaaatca tgtaatacag cttggtacat gatttttatt
      181 tgtagctgtt ttttacatag acacaaaaaa gaaaccacgc ttgtagacgt ggtttctttt
      241 ttactatctg tttccagaag ctgaggtgct gctggatggt gtatggtcat ttgcagcatc
      301 cgctccgcta gttgctggct cactggaaga agcaggagga gcagatgaag acggtgtcga
      361 gctttcttcg gatgaagaag aaggaaccgt tgttgaagac gctggctcgc ttgtttctgc
      421 tgtcgtgctt tcttttgagg actcagcagt tgattcgctt tgactagtag agctagagct
      481 ttgagtcgtt gtctctgttg ttgagcttcc aggagtctgg acatagctgg aagatggaat
      541 agttgttgat ggtgtaattg cattcgatct agctgtatat tgatctttgt agtacagttc
      601 accgcccaca cgaattaatc cgcttggcat ctcccagtct gtatttgtca cgttagctga
      661 aacatactgc ataagctcgc gataaacatc agaggctacg tgtgacgatt ctgaagtgac
      721 aggtgtcatt tttttattgt aacctgtcca cacggaaatc gaataatttg gcgtatagcc
      781 cgcaaatagg atatctggat acaccccaga agaaatacca agttttgcat actcgtcatc
      841 tgtgtaattg gatgttccgg tcttaccggc ttgatataac ccagcgattt gcgcattcgt
      901 accagttcct tcagtgatcg tatcttttaa aatgtctgtg atcatataag ctgtttctgg
      961 agacatggct gtttttccat ctggctcata agtttcttct gttccgtctt ggaagacgat
     1021 tttattaaca tattgaggtt tgtaataagt ccctccattg gcgaaagccg cataggctgc
     1081 tgccattttt aaggaagaag ctccgtattt tgtcccatct tgttcttccg tattactaga
     1141 gatcgcattc gattgatgga tcgtactgta ttcgatccca agatttttta agaaagaagc
     1201 cactttatct gaaccgactt cattgaataa tttgactgct ggaacattac gggataagta
     1261 aagtgcttgg cgtaaagtaa tggtgcccat atattgatta tcccagtttc ctacaggagt
     1321 cgaagtccct tcatagttat acggtgcgtc agtaatagtt tttccagtag aatatttcaa
     1381 atattcaaaa gctggtccgt aatctgtgac aggtttcatc gtagaaccaa aatcacggga
     1441 agtattcact gctaagttgt tgcctaaagt tacgtcttct gcgatgtgac gtccgccaat
     1501 ctgggctttt actttcccgg tattggtgtc gataagggtg gaggcaactt gcatctcgtc
     1561 gtctgggtaa gagacatact gatccgtgtt gacgatatca tatagtttct tttgggcgtc
     1621 cagatccaaa ttcgtataaa tctctaaacc gtctgtgtag acattcttat ctgttttttc
     1681 ttgtacttca ttgatgactt ctttgacata attatcgacg atcttagtat tgtcatcgga
     1741 ttgagttagt tcttgcagac catccgttac cggtacatta acagcttgat catattctgt
     1801 ttgagagatt ttttcgtttt gaagcatcgt atacaaaaca gtatctcgtc ttttcttcgc
     1861 ctgatctgga taaacataag gatcataggc ggaaggggct tgaggcatcc cagcaagcaa
     1921 tgctgtttgc ggtaaagaaa gttctgataa tttcttacca aagtacatct cagaagctgt
     1981 ctccatcccg tacaagccgt ttgacatata cactttattt acgtagtagg tcaagatctc
     2041 ttgtttcgat tttttttgtt ccaatcttac agccatccac gcttcttggg ccttgcgttt
     2101 cagagtctga tcctcggcac tggtggagaa gaaggaaagt ttgatcaatt gctgtgtcaa
     2161 agtacttccg ccttgcagtc cgccagaggt aaagttagag agggcagaac cgatgatccg
     2221 aatagggtca accccgatgt gtttataaaa acggcgatct tctaccgaca cgatcgcatc
     2281 ttctaatgtt ttcggtaatt cattcgcaga aattttttca cgcttttctg cgcctaaatc
     2341 ttcgaatagt tcgccatctt gtgtatatag tttggaagat acggtagcgt ctaatttttt
     2401 atccgtcaat tcgggcgcat cttttgcgta ataccagaaa agtcctacac ctgctaaaaa
     2461 gaggatacag aaaaatgaga gtattcctaa aaggatcttg ataaataatc cttttttatg
     2521 tgttccagag gactttccag aacctttgcc agaattcttc ttcacactct ttttaggagt
     2581 aggctgtttc tgtcgtctag agcttctagt ttgttcattt gccattcttg ctttctcctt
     2641 caatagataa tgttgataaa tagaagtcaa ccgcgtccaa ataaggaatc cgcggagaaa
     2701 taccagtttt gatctctatt cctagttctt cgatcaaaga taaaggtagt gatttgcgtc
     2761 ctttttcctt ttgggcgtcc cagtaagcaa tcaaatgaac actattcaaa taaaaacagc
     2821 gcttcaaaga cgaaaaccat aacaagacaa agcagacccc attttgttcg aggcatgctt
     2881 tcatatgttc gatttgatgg gcatggaagt ttttaaaggg aaaagatgtt ttgttcttgg
     2941 tttcttttgc ttcaaagtcc aagtaaaagc ctcgatagac gccattatag tctgtagttg
     3001 aggcttgggt aaagtaggct tccttgatca cagcagcact tctcttagga tagtctactt
     3061 tcacgatttg gataggggta ggctttttat ggataaccgc caacccgcgg gaaagataat
     3121 atttattact ttcattgatt gcttcttcaa agcgcatccc ccgattgccg aattcagtga
     3181 tctgatttct tttttcttga attttttcga cgggagaggc attcttataa taaggttggc
     3241 catttggata gcgcaatacc atctttaatc acactcctag gccacattat agcaaaaaag
     3301 cggatagaaa gaaaaggaat ctgagatgga taagtcaaaa gtaatctatg tgtctgggta
     3361 tcgaagtttc gaactcggtg tttttaaaga aaatgatcct aaaatccaag ttgttaaaaa
     3421 tgttttaaaa aaagaactcc atcagttagc agaagaaggg ctggaatggg tacttgtcag
     3481 tgggaatttg ggcgtcgaac tatgggctgt gcaaatagta gcggaattga aagaagaata
     3541 tccggaacta tctcttggta ttttgtatcc tttttctgat tttggtaata actggaatga
     3601 agggaaccaa gaaaaaaaac aattagcaga acaattagca gattatgtcg aagctgttag
     3661 ccatcaaccc taccaatcac ctagtcagct aaagaatcac actcgtttta tacttgagca
     3721 tacagtgggc tgtctgctga tctatgatga agaattccct ggaaaaacga agtatttttt
     3781 tgaagatgca cagaattacc aagcagatca tccctacgaa attcggctga tcagtatgga
     3841 cgatcttcaa aattttagtg aataatcgat attgtgtttg atttatcaac ttttttccta
     3901 tacaatgaaa aaggtagaaa tgttattacg taaatgaaaa ctagaggtgt aaatatggcg
     3961 aatttggttt atagtcctaa agacatttta caacaagaat tcaaaacaaa aatgcgcggc
     4021 tatgatcccg ttgaagttga tgaattctta gacaatatca tcaaagatta tgaaacttac
     4081 agcaaagaac tccttgcttt acaagaagaa aacgatcgat tgagtgcaaa agtagctcaa
     4141 ttatctaaaa ctcaaggagc agctcaaaca cgcgtgcaac aaacagaggc accaaagagc
     4201 gcagcagtga ccaattttga tatcttaaaa cgcttgtcaa atcttgaacg tgaagtattt
     4261 ggtaaaaagc ttgatcaaca agcaagtgca gtcaaaccag cacaacctaa tccaaataac
     4321 tacacaaatg cagacacaag cttagatgat aatgaaaaaa cacgtcaatt ttaatttttg
     4381 attaatgaat ttgcggtttt cgggtaatcg cggtctgact tgatcaggct gaggaaagtc
     4441 catgctcgca caggctgtga tgcctgtagt gttcgtgctt agcgaaacca taagctaagg
     4501 tactctttgg agtaacggca ggaaaacaag ctaaggcttc ggctatgctt aagtatcctt
     4561 gaaagtgcca cagtgacgaa gcggctgggg aaacccatcc ggtggaacgc ggtaaacccc
     4621 tcgagcgagc aacccaaaca atggtagggg cgctttttgt ctggaagaag aacaggacaa
     4681 aaaggcagta taactgcaga tagatggtta ccgctttgtt ttttctccct ggaaaagcaa
     4741 agtacagaac atggcttata gaaaaccgca aacatcaggg aattaggtcg tgacaaaagt
     4801 aatgcgttat aagaaacaag aaaaacattt gaaagctatt tttcaatact tcagatgaat
     4861 cgttcttatt acaaaacaaa cttttgtatc accggttatt cggaatttga gtaaaagtgt
     4921 ggggtaggac tgatgtccca cctccttttt ttataaataa ggagattgtg tatggaaaaa
     4981 aacaggaagt tcaacctcgt cgcaactgcg gcaagcgggc tagaagcact ggttggtaaa
     5041 gaattaagag atcttggaat cgaatgccaa gttgagaacg gacgtgcacg ttttgaagga
     5101 acaatggaaa cgatcgctac agctaattta tggttacgta cagcagatcg agtaaaaatc
     5161 gtggttgggg aattcgatgc gtatacgttt gatgaattgt ttgaaaacgt aaaagcattg
     5221 ccttgggaag attttcttcc actagatgca gcttttcctg tagctggaaa atcgatcaag
     5281 tcgaaactat atagcacacc tgattgccaa gcgatcacaa aaaaagcaat cgttgaacgt
     5341 ctccgtacct attatcaccg tccagcttct gtaccactga cggaaactgg cgcacatttt
     5401 cagttggaag tagctttgct gaaagaccat gtgatggtaa ctctggatac gacaggacca
     5461 agtttattca aacgtggtta tcgcttagaa aaaggtggag caccattgaa agaaaacatg
     5521 gcagcagcac ttattatgtt gacaaattgg cgaaaagacc gtccatttta tgatcctgta
     5581 tgtggttcag gtacgctttg tattgaagca gccttaatag gccataatat cgcaccagga
     5641 ttcaatcgtg agtttgcttg cgaatcttgg gattggttca gtcaagaaat catggaaaat
     5701 gttcgatcag cagccgaaga aaaagcagat tatgatattg aattagatat tactggttca
     5761 gatatcaatg gacggatgat tgaaattgcc aaagccaatg cagaagaaat cggattaggc
     5821 tcttcgatta cctttaagca acaagcagtc aaagacttca aaacggataa agaatacggg
     5881 gtcatcgtgg ctaatccgcc ttacggagaa cgtctaggag aagaagaaac tgttcggaaa
     5941 ttatataaag aaatgggcga tgtattccgt ccattaaaaa catggagtaa atatatcttg
     6001 actagcgact tagcgtttga ggaattttac ggacaaaaag caaccaaaaa aagaaaattg
     6061 tacaatggtg cattgcgtac ggatctattc cagtattggg gaactcgtcc gcctagaaaa
     6121 aaagagaaag acgaataatc ttactaattg attcattcaa ttggatgttt ggaggaatag
     6181 ggaatgaaag aaaaagagtt tttacaagaa gtaaaagaaa tcaatcttct acaacaagct
     6241 ttaggaatcc tcgattggga tacacaaaca ggtatgcctg aaaaagctag tgcataccga
     6301 agtgaagtag acagctatct ttacagcatt tatttttcaa aaaaaatcgg tccaaaaatc
     6361 aaagaagcca ttcgctattt ttctgaacat ccggaagaat tgtcggaagt tggaactgcc
     6421 gtttttgcca aagtaaaaga agagtatgat ttggaacatc gcgtaccgga agatcttatg
     6481 agaaaatata cagctgccgt ttcttcagca cacggacaat ggcaaaaagc tagagaaaca
     6541 caacaatttt ctgattttca acacgcattg aatcaaaata ttgaattaac aaaacagttg
     6601 ataccttact ggagaaaaga tgaaaagacg aattatgatg ttttgctgaa tcaatacgaa
     6661 cccgggatga ctgtcgaaat ccttgataag gtatttgatc agcttaaaga aggaattctt
     6721 tcgatccgcc gtacgctcca agaaaaaggg actgaacctg aaacagactt tttaaatcgg
     6781 agaatgacga aaaaacaaca gaagagattt gtagtgaaag taatcgaaca acttggttat
     6841 gattttagtc gtggtcgttt agatgacact gtccacccat ttatgactgg aattaatccg
     6901 aatgatgcac gaatcacgac acgatggaac gaaacagatt tcaaaatggc tgtttttgga
     6961 gtgatccacg aggctggtca tggtatgtat gaacaagaca tcgatgataa atatgcgtat
     7021 acaccggttt atgaaggtac atcaatgggt atccatgaat cacaatcgtt gtttaacgag
     7081 atcattattg gaagtagtcg cagcttctgg aaaaagcaat atcctttctt tcaagaatgt
     7141 gcagaaggaa cattcgatga tattcctttt gacaaatttt atcgttcatt gaaccataca
     7201 aaatcaagtt tggttcgtat cgaggcagac agtttgactt accctctcca tatcatcatt
     7261 cgttatgaaa tcgaaaaaat gattttcaat gaaggggtag atgttgctga actacctgaa
     7321 atatggaatc aaaaatatga agagtatctg ggaatacgac caacaaatga tgtggaaggc
     7381 atcctacaag acgttcattg gtcaggtgca agctttggtt attttccctc ttatgcatta
     7441 gggtacatgt atgcggcaca attacttcat tctttgcagc aagaaataga tgtggaaaat
     7501 gttctagcgt cagacgatta ttctccaatc aaaaactgga tgtcagagca tatccatcaa
     7561 tatggtgcat ctagaaaacc aaatcaatta atcatggatg caacacatga accgcttaat
     7621 ccgcaatacc tgatcgacta tatgaaagac ctgtatttta atgtatatca ggtaaaataa
     7681 aatacaattt acaaagatag ctgaaattca tctaatctat agatgttttc agctatttac
     7741 ttaagaaaga agtggaacga atggataaat tacaagtgat cgctgattat agtatggacc
     7801 aattagcgca tgatcaaaca ggacatggga gcgatcatac caaaagagtt gtcaagttag
     7861 ctgaaaaaat tttggagacg gaaccgcagg cagaccgctt tgtgacgctt gcagcagctt
     7921 acctacatga tacgattgat gataaagtcg ttaaaaatga gaacgaggca aagcaacagc
     7981 ttcgtgtatt ccttcgctct ttgccaatag tagaggaaca gatctcgatg attttttcca
     8041 ttatcgaaaa tatgtccttt tctaaaaatc tctctgaagc ggccgaacta tctctggaag
     8101 gtaagatcgt tcaagacgct gatcgtatcg aagcattagg agcaatcggc attttgcgta
     8161 ctgcttattt cggcggtgga catgggcacc cgatttttga tcctgaactg tatccgcaaa
     8221 cattcaagga caaagaaaat taccgaaaag gcacgacagt gatcaatcat ttttatgaaa
     8281 aactgttctt gctgtctgat aaaatgaata ctgattacgg atatgaagaa gccaaacgaa
     8341 gagaaacctt catgcgtgaa ttcttagaag aattttttct ggaatggtct attgacgatg
     8401 aaaattttac tccagacaat tggatcaaaa ataacaaaaa atgatggagg ttgtgacaga
     8461 agtggtcagc tccaagatat ttcggatcat tttccgtaaa agttacttct ggaacactgt
     8521 ttaccaagag gtcgtgagcc tgccgttcag ctccgagtca caaggaacaa tttcaaaaaa
     8581 tagcttctcg tgtttgatga atattgtaac ttgtgattcg gaattggcag gagaacaccg
     8641 tttattcgga agaataggac tgtgacaaga cttttgtcac agtccttttt tcgaattcaa
     8701 caagtaaaac ttttaagaaa tattcagaga gtaaaattaa aaattgctga taaacgaaga
     8761 tggaaatttt atgtattttt attggtctat gcaataaata aattaaatat tcagaataca
     8821 attgatttta acgaaagggc ttctatcaat ggttgtgttg tatttatctg cccaggtaga
     8881 gtaaaataga tcattatcta aaaggtaatt gttcatacta tataagtcat ttgaaagtaa
     8941 aaagatactg taaataaaaa atgtggttaa ataattctca taatcagtgt aagcgttatc
     9001 aaaaatatag catacttgct ttgtagatga atgacacgat atatgtttgg tatgttctgc
     9061 aagacagatg aatatggagg tggagacaat gtcttccaaa gaactaacaa tgtatttaga
     9121 gaaagaattg gatcaactga aagaaaaggg gttatacaat acgatcgaca tactagaaag
     9181 tgaaaatggt gcttgtatca tcgtagacgg aaaaaagatg atcaatctag catccaacaa
     9241 ttatcttgga tttgccaatc gcgaagaatt gaaaaaagca tgtattgaag caaccgaaac
     9301 ttatggtgta ggagcgggag cagtccgaac gatcaatggt tcgctgaaaa tccatcagca
     9361 attagaagaa aaaatcgcag aattcaaagg gacagaagca gcaatcgcct ttcaatctgg
     9421 attcaactgc aatatgggcg ctatctctgc agtaatgaca aaagaagatg ctatcttatc
     9481 ggatgaatta aaccatgcat ctattattga cggttgccgc ttatccggag caaaaatcat
     9541 ccgaattaaa catcaagata tgaaagacct tgagaaaaaa gcaaaggaag cgatagaaag
     9601 taagaaatat aaaaaaatca tgtatatcac agatggtgtt ttctccatgg atggagacat
     9661 tgcacgattg ccggaaatta ttccgattgt tgaaaaatat ggactgatca cctacgtcga
     9721 tgatgcgcac ggctcaggcg taactggaaa aggtgcagga accgtcaagc attttggatt
     9781 aagcgacaaa atcgatatgc aaatgggcac gttatcgaaa gctataggtg ttgttggggg
     9841 ttatgtagct ggatcgaaaa cactaatcga ctggttgaaa gctagaagtc ggccattcct
     9901 attttctact tcgctaactc caggtgcagc tgcagctgca ttggcttcga ttactttgat
     9961 gcaagaacat ccggaacttg tcgaaaaagt ttgggaaaat gctaattatt ttaaagaaga
    10021 attgaaaaaa gtaggctata acatcggtat gtctgaaaca cctattactc cagtcatttt
    10081 gggagacgaa aaagtgactc agactttctc taagaagctg atcgaacatg ggatttatgc
    10141 taaaccaatc gtttatccaa ctgtgcctct tgggacaggc aggatcagaa atatgccgac
    10201 tgcggaacat acgagggaaa tgcttgatga agcagtagca gtttatcaaa aaataggaaa
    10261 agaaatggaa attatttaac taaatttagt tttttcgtct atttaaaatt agagaacatg
    10321 ttcacgcata tattgttaat catctgaaga tagtagtaaa aaagatcagg gaggatcatc
    10381 atgaaaaaag tattggtgac aggatgttta ggacaaattg gttcagagtt gatcacacgg
    10441 ctacgtcaag aactaggcag tgaaaatgtc attgcgacag atatacgcat gcctgaagtc
    10501 attgagaaaa acgatatctt tgagatccta gatgtaatgg attacgaaaa aatgcgcagc
    10561 atagcagaac aatatcaagt ggacacattg attcatttgg ctgctctttt gtcagctgtt
    10621 gcagaagcaa aaccacaatt agcctggaat ttgaacgtga ctgggctggt caatgcattg
    10681 gaagtcgcta gagaattcca gctgaaattt tttacgccaa gttcaatcgg cgcgtttggt
    10741 ccaaatactc caaaagatca taccccacaa gatactcttc agcgtcctac aaccatgtat
    10801 ggggtaacga aggtagctgg agaactacta tgtgattatt attatgtgaa atatggcgta
    10861 gatactcgtg gtgtccgttt ccctggattg atttcttatg caaccttgcc aggaggcggt
    10921 acgacagact acgcagtcga tatttattat tcagcaatca aagaaggaaa gtacactagt
    10981 ttcattgatc aaggtacatc aatggatatg atgtatatgc cagatgcgat cgatgccatt
    11041 ataaaactca tgaatgcaga tcctgatcga ttggttcatc gaaatgcctt caatatatcg
    11101 gcgatgtctt ttgacccgga agagatcaaa gcatctattc aaaaaatcat accagatttt
    11161 gaaatggatt atgatgtgga tcctgtacgt cagtcaatag cagaatcttg gccaaattca
    11221 ctagatgttt cttgtgcgca agaggaatgg ggcttttcgc cgaaatacga cttagatgtg
    11281 atgacaaagg atatgttgga aaaactgaaa gaaaaattgg tagagacggt ataaaacaag
    11341 aaccacatcc aaaaagtttt ttatactttt gggattggct atggtatgaa aggggacaag
    11401 ggatggaaga atatcaagcc acgccagtaa aagaagtaaa aatagaaaat gaattaaaac
    11461 gtacgatggg attttttact gctttatcga ctgttatggg cacagtgatc ggtgcagggg
    11521 tattttttaa agccgcaagc gtagcagaag ttacaggatc cgccagtttg catatgtttt
    11581 cttggtttct cggtggtatg atttctgtct gtgcaggact aacaggagct gaactggcag
    11641 ctgctattcc agaaacagga gggatgatta aatatatcga acggatttac ggaaatacag
    11701 cagctttttt attgggctgg gcgcaagtcg tgatttattt tccagcaaat gtagcagcct
    11761 tgtcgatcat tttcggtaca cagttcgtca atctatttgg tctttcccag tcaatgattg
    11821 ttcccgtagc tgttacagct gctgtatcga tcctgttgat caatttttta ggttctaaag
    11881 ctggaggggc ttttcagtcg attacgcttg tctgcaagct tattcctttg tttgtgatcg
    11941 tcattttcgg tttgttccga caagaaggag tagacttcca actgtttccg atccaagcag
    12001 gagaaaatct ttcatttttc tcagccttgg gagcgggctt gctagcaacg atgttcgcat
    12061 acgacggttg gatacacgta ggaaatattt cgggagaatt gaaaaagccg gcgaaagatt
    12121 tgccaaaagc aatttcttta ggaatcatcg gaatcatgat cgtttattta ttagtaaatg
    12181 cggtgttctt aagaaccgca tctatcgacg gagtcgcagg aaacagcaat gcggcaagtg
    12241 acgttgcaaa aatgattttt ggcggcttcg gtggaagact ggtcacagtt ggtatcttga
    12301 tttctgttta tggaaccatc aatggctata cccttactgg aatgcgtctc ccttatgtta
    12361 tggcaaaaga aaacaacctg ccttttagca agctctttgc taaacttcat gataaaacta
    12421 aagttcctgt agccgcaggt atcttagagc tagtcatcgc tattggcatg atgatgatcg
    12481 gtggttttga tacactaacg gatatgctga tctttgtcat ttggattttt tatacgatgg
    12541 tctttgttgg tgtcattctt cttcgtaaaa aagaacctga tttgtttaga ccatacaaag
    12601 ttccaatgta tccgtttatt ccactagtag caatcattgg agggactttt atcgtatcaa
    12661 gcacgcttat cactcagacc tttttagctt cgatggggat agtattgaca cttgctggta
    12721 tcccgattta tctctattta aaacgtagat agctaaaaca ttcgaatttt attagaactg
    12781 cacttctctg ccaaacaaaa tattttattt aaatgaggag tacgtggaag aaatgtcaaa
    12841 agtactcgtt aattttgatc acgtaaatgt cgttttgtct acgatgaaat agcaaagtaa
    12901 aagacagaaa tcgggctcaa ccgatttctg tcttttactt tgatttgcgt aactgaacta
    12961 ttcggcgtac gccgcgataa atatttaata aaaccaagca accagaaatg aaaatgggaa
    13021 gccaaatcag atagacaggg tagccaagca ctttagacaa agcatgtgga gtcattttat
    13081 ccatttcaag aagtgcttgc tgccaagtct gaagattgaa gactagcatg aaacccgaaa
    13141 taatcaggag aataaatcct aaataatgaa ttgtagttct ttttcttaca taagcgatcg
    13201 tagcataaag gaacagtaaa aaaataggta cgccaaaaac aaagtacata ttcagcatct
    13261 cgacaccccc tttgtgttta tcttacttta ctttgaaaga aatgagaaat aatagcctcg
    13321 ttacttgctt gaaacagcaa aggactgcta agataagaga gatacaaaga atcaaggagg
    13381 agtcatatgg caagttatat cgaaccgatt cgttttgcgc tgattgtttt tccattttta
    13441 gccttagcga tctcatctgt tttttttatt tatgaatatc gaaagtatgg aacatttcta
    13501 gtaacaaggg cagttatttt gtattctttc gttttttatt tattgtgtgc gtattttttg
    13561 gtgatcttac cacttccggc aaaggagacg gttgcccaaa tgacagggcc aaagatggag
    13621 ctgcatttat ttgcttcatg gcaacatttc agagacaaca cggtattggt cttgtctgaa
    13681 cctagtacct acctgccggc aatgaagcag agtgtatttt tggaaccagc ttttaatgtt
    13741 ttattactca taccatttgg catttattta agatattatt tccggctttc ttttatgaaa
    13801 acgatgattg ctagtttctg cctttctcta ttttttgaat tgacgcagct aagtggactt
    13861 tattttatct acccaagacc ataccgctta tttgatgtga atgatttagt gacaaataca
    13921 ttgggtggaa tcattggttt tgtcgttacg ccgttattta catttatgct tcctacaaga
    13981 agacgaatgg atgaactttc ttatgaaaaa ggaaagactg tctcattgac tcgacgcttg
    14041 gtcgcttatt tgatcgactg gacattttta tctcttatta atttggtagc ggcgattttc
    14101 tttaaaattg cgttcgattc aggggcagtt acttctcata tctggtggct tttattagaa
    14161 gtaggggtat actttatgat tgtaccgttt ataaccaatg gatggacact tggcaaaaaa
    14221 attgtgcgta tccgtttggt ggaagaagac cgcgaacgaa tttctttaaa agcattgatg
    14281 atccgctacg ggtatctgta ctttcttttt tatggtttaa gctggttggg gcagttgctt
    14341 cagcaaatca tgaattcgac aacgaatacg gtgttgcaga tgagtgcagt attcatttct
    14401 ctcggtgtct tgttactaca aggtattttt attttaaata tcttgctatc atttttgaga
    14461 aaaaaacgga ttctgtttta tgaaaaagct agtcacacat atactgttag cacgattggc
    14521 caaacaaagg atgatgattc tcgaaattag ctgagcaaaa acgctttgtc tgtgttataa
    14581 tgccaaagtc gtgataattt aaagagaaga ggtgcacgaa tgaataattt tgatatattc
    14641 cgcttcaaac cgtttatcaa tgaagcgtta accgcaaagg gcttcaaaca gcccacagag
    14701 gtccaggaac gtttgattcc tttaattgca aaaggaaaaa atatcattgg gcaatctcaa
    14761 actggtacag gaaaaacaca tacgtttttg ttgcctttgt ttgaaaaaat caatgcagaa
    14821 aaagaggaag tacaagttgt tattacagct ccaagtaggg aactagctgc acagatctat
    14881 gaagcagcta cacaaatcgc taaattttct acaccggaga tccgtgtctc gaattttgtc
    14941 ggcggtacag acaaacagcg tcaattaaat cgtctgcatc accaacagcc acaggttgta
    15001 gtaggcacgc ctggtagaat attagacatg atgaatgaac aagcgctgaa gattcacacc
    15061 gcatttgcat ttgttgtcga tgaagcggat atgacattgg atatgggttt tttaaatgaa
    15121 gtagaccaaa ttgcaagtcg attaccagaa aagctgcagt ttttagtttt ttctgccact
    15181 attccagaaa aacttcgtcc attcttacga aaatacatgg aaaatccaat cattgaagaa
    15241 atcaaaccaa aagcagttat ttctgaagac attgaaaatt ggctgatttc aacaaaaggt
    15301 aaagatgaga atcaaatgat ttatcaactg ctgacaattg gtcatcctta tttagcaatc
    15361 gtatttgcca atacaaaaca gcatgtggat gaaattgccg attacttgaa aaaccaaggc
    15421 ttgaaagtag caaagattca tggagacatc accccaagag agagaaaacg agtgatgcga
    15481 caagtacaga atctggagta tcaatatgtt gtagcgactg atttagctgc tagagggatc
    15541 gatatcgaag gtgtctctca tgttattaat gcagaaattc cagaagatct agacttcttt
    15601 atccatcgtg tcggacgtac tggacgtaat ggcttaaaag gaacggccat tacgttatat
    15661 tcgccaaatg atgagcaagc cattgatgcg atagaaaaat tgggcgtctc atttgaacca
    15721 aaagaaatca aaaacgggga aatcgtcaaa acatacgatc gtaatagacg tgccaaacga
    15781 gaaaaatcaa aagatgtatt agatcccacg ctgattggtc ttgtgaagaa gaaaaagaaa
    15841 aagatcaagc ctggttataa gaaaaaaatc gattgggcaa ttgcagaaaa aaataaaaaa
    15901 gaacgcaaaa tcgaacgcag acagcaaagc cgtactgctc gtaaaaataa gaaaaattca
    15961 cataaataaa aaacaagagg ttgtgacaaa aggcgtgtca caatctcttg tttttaagct
    16021 agactttttt tcattccgat acaaagtact tcatcttcaa taaaaggttc tgtgcaagta
    16081 tgataccctt gtttttcata aaaatctaca gccgtcattt cagctactac ataactagtc
    16141 tgacaacctt ctgaaaccgc ccgtttttca gcagcttcaa gcaactgacc gcctaaccct
    16201 tttcctcggt attcttttgc tacgcaaaat cgttccggct tgaagcaagt aggtgttttc
    16261 ttttcataac gcaaagtagc tactggtctt ttttgatcaa gaaatagata ataggtttgt
    16321 tgcgggatat cttttccgtc gaattctagt tctgcaggaa tcccttgttc taatacaaaa
    16381 acttgatatc tcaaaaagca gctagctgcg cgtatccatt catccgctcc aactattttt
    16441 ttcatcatat tcctccttgt catgaatagc agagatgtat tgattccttt attcttatcg
    16501 taccaaaaat cgtggagtat gaccaaaagg aaatgaaaat aaaaattgag atattgacag
    16561 tgatcaatag aatgcgtata ctaattaagt acgaaaagga tatgatttgt tttttaagac
    16621 tcacagaaaa ttctttcttg gctgaaaaaa gaatagtcac taatcaaatt gctccctttt
    16681 tctattttgg tagaaatacc gacgcagctc agcgttatcg agtttaagag gtaatgatta
    16741 cacatcattg caatcaaggt ggtaccgcga gttttcgtcc ttggctgtga tggtgttttt
    16801 ttgtatttaa tttatagaaa gcaggtaatg acatgaaaca attaagtagt gcacaagttc
    16861 gacaaatgtt tcttgatttc ttccaatcta aaggacatac gattgaacct agtgcgtctt
    16921 tgatccccgt gaacgatcca accttattgt ggatcaactc tggtgttgca acattgaaaa
    16981 aatattttga tggttcagtt gtgccagaaa atccaagaat cacaaatgcc caaaaatcta
    17041 ttcgaacaaa tgatatcgaa aatgtcggta aaacagcacg gcaccatacg atgtttgaga
    17101 tgctgggtaa tttttcgatt ggtgactatt tcaaaaatga agccatccac tgggcatggg
    17161 aatttttgac tgatccaaaa tggatggcat tcgatccaga aaaactttat gtaacagttt
    17221 atccaaaaga tacagaagca aaacgcatct ggcatgaaga agttggcctg ccactagatc
    17281 acattattga tgtagaagac aacttctggg atatcggtgc aggcccatgt ggtccggata
    17341 cggaaatttt ctatgaccgt ggagaagctt ataacgattt agcagaagac gatcctgaaa
    17401 actatccagg aggagaaaat gaacgttatt tagaaatctg gaacttagta ttttcagaat
    17461 tcaaccataa gcctgataac acatttgaac cacttcctca taaaaacatt gatacgggga
    17521 tgggattgga acgcatggtc tcaatcgttc aagatgcgtc aacgaacttt gagacagact
    17581 tgtttatgcc tattatccac gccgtagaaa agctaagcaa acaagtaaaa tatggtgaaa
    17641 acccagtaac agatatttct tacaaagtaa tcgcagatca tattcgcgcc ctttcatttg
    17701 ctattggcga cggtgcattg ccatcgaatg aagggagagg gtatgtctta cgccgtttgc
    17761 ttcgtcgtgc tgtcatgcat gggaaaaaat taggtattga acaagcattc ttatatcgtt
    17821 tagtccctgt tgttggggaa atcatggtaa gttattatcc tgaagtactt caacaaaaag
    17881 aatttattga aaaagttatc cgtacagaag aagaacgttt caatgaaaca atcaatgaag
    17941 gtttggaaat cttaaatcaa gtgatccaag aagtaaaaaa cgaaaacggg acgactctag
    18001 caggaaaaga catcttcaaa ctatacgaca catatggctt cccggtagaa ttaacggaag
    18061 aggtggcaga agatgaagga ctgaaagttg atcatgctgg ttttgaaaaa gaaatggatg
    18121 cacaacgcga acgtgctcgc tctgcccgca gtactgaaaa atcaatgggg gtacaatctg
    18181 ctttattgac agatatcaaa gtagaaagta aattcattgg atacacagaa caagaatcag
    18241 atagtcaatt attggtcatc gttaaaggtg agtctctttt gaatgaagtc gcagaaggtg
    18301 aagctcaaat gatctttgat caaacacctt tctatgcaga aatgggtggg caagtagcgg
    18361 atcaaggatg gatcatgaat gaagtaggcg aaatcgtagc agttgttaca gatgttcaaa
    18421 aagcgcctaa tggtcaattt atgcacaccg tagaagtaaa agcaccaatg agtgaaggac
    18481 aaacctatca tctattgatc gatcaatcga tgcgcaatcg gatcatcaaa aaccatacag
    18541 caacccatct actgcacaag gcattgaaag aagtacttgg cgagcatgca aatcaagctg
    18601 gttctttagt cgctcctgga catttacgat tcgactttac ccactttggt caaatcactt
    18661 cagatgaatt agctgaaatg gaaagaatcg tcaacgaaaa aatctgggaa gctatcccag
    18721 ttgaaacaat tgaaacagat atcgatacag ccaaaaacat gggtgcaatg gctttgtttg
    18781 gtgaaaaata cggacatgac gttcgagtag taaatattgg cggctggtca atcgaactgt
    18841 gcggaggcac acatgtagca aatacagaaa acatcggaat cttcaaaatc gtttctgaat
    18901 caggaattgg tgcaggcgtg cgacgaatcg aggctgtaac aagtaaagaa gcgtacgaac
    18961 tacttgaaga agaagaaaaa caactaaaac aagtagcaac aattgttaaa tcacctcaat
    19021 tgaaagaagt tgtttcaaaa gcggaacaat tgcaacagca agtaagagag ctacaaaagg
    19081 aaaatgaaca attagctagc aagttagcga atcaacaagc tggtgatgta ttcaaagatc
    19141 cagaaacgat caatggaaaa acaatcattg cagcacaagt aaaagtgaaa gatatgaatc
    19201 agctgcgtca attagctgac caatggaaac aaaaaaatat ttcagatatt cttgttttag
    19261 caaacgaaca agaaggaaaa gtcagtctgc tagcggcaat gacgaaagaa tcaaatgaag
    19321 ctggactaaa agctggcgac ttgattaaag caatcgcacc gaaagtaggc ggtggcggtg
    19381 gtggacgtcc agacatggca caagctggcg ggaaaaatcc tgctggtatt ccagatgcac
    19441 ttaaagctgc gaaagaatgg gttgcgcaac aatagggcgt ttacgtttga cagaaaaaca
    19501 aaagttcaaa cattgaacga aacatgatta tgggggcgaa gacaaacaag tcttgccctc
    19561 atactttttt caattggatg gcgcatctaa tcaaatggtc ggattcggat ggtctttagt
    19621 tgacgaaagt gacagaaatt tttacgatta gtataaataa aaaggggtga aaaaatgaat
    19681 agtagaaata agacctttcg actagtctta cgtgcgatct tgttagcaat catcatcgta
    19741 caagcgatgg ttccttggct ggggtttatt ccattaggat tcattagttt aacaattatc
    19801 catatcacag taattattgc tgcagtagta ttaggcccaa aagatggcat ggtgattggc
    19861 ttgttttggg gaatcgctac aattgttcgt gcctatgcca tgccaacaac gccatttgat
    19921 acattagtgt tcacgaatcc gattatctcg gtagttccca gagtacttgt tggcttagta
    19981 gcaggcttgg ttttccactg gatttatcaa aaaaataaat caataacgat cagctcagcc
    20041 tttgcagggg tactgggttc attggtcaat acagtcttag tgttaggatt catgggatta
    20101 ttttatacag gtgcaacggc agatgcctat ggtgtagact ctgctctttt atttaaaacc
    20161 ttagcagggg ttgcagcaat caatgggata ccagaagcaa taggtgcggg aatcataaca
    20221 ccattactag caaaagcgtt atttgcagca acaccgttga aaccagaata atgaccatct
    20281 tttaatagta ataggacgca cgatagatag aaaaaggaag agaggagagg aaaacaatcg
    20341 tcctctccat ctttttacct gagcaacaag caaactagaa ggggtaaaaa acttggctaa
    20401 gaaacaagaa gaaaaaaaga aacaaatacc gcaacagcgg acaaaaaaag gagtactagc
    20461 gggtatctta ggattggtta cgaatctgat cttgtttgtc tctaagctaa tgatcgggct
    20521 tttttcagga agtgtctcca ttatggctga tgcagtgaac agtctatcag atactgcttc
    20581 atccatattg actttgatcg gatttaaaat tgctgcaaag ccagcagatc aggaacatcc
    20641 ttatggacac gaacgcttcg aatatatcag tgggttattt gtttcgatca tcattactta
    20701 tgtaggattt caatttttag agtcttctgc tgaaaaaatc atccatccag aaaatgtctc
    20761 tttaacaccc atcgtctttt ttgtcctaat catttccatc tttttgaaat ttgcacaagg
    20821 caaaatgtat caggttattg cgcaatccat tgattctgaa acattgagag ctacagcaac
    20881 agatagttat aatgatgtct ttactacact ggctgtattg ctttcagcag caatcgaatg
    20941 ggtaactgga tggcgtattg atggatatat aggttttgct ttagcattat atatccttta
    21001 tagtggattg acgatgcttc gaaattttgt atacgaacta ttaggaagtc gcccaacgcc
    21061 agcagagatt gaagaaatgg aagagcattt aaatgcttgc gatgatatac ttggctatca
    21121 tgatttattg gtgcataact atggacccaa taaacgcttt gcatcagtcc acatcgaagt
    21181 agatgaatca atgaatttga atgtggctca taagattatt gatctgatag aaaaagactt
    21241 taaaaagtct ttggatgtgg aacttgtctg tcacttggat ccaatgcctg ttgataacga
    21301 aaaatactat aatatcttgc ggcagttaaa gcaaattctt cttgaaacag gtacagggct
    21361 taatattcat gattttagag taattcatca aggaaaaacg ctgcaatttg atgtagtggt
    21421 ccctgagaaa ttccagtttg ttgatacaga actagaaaga atcttacgta gtcaaataga
    21481 agaaaaaatc ggagattacc aattggaaat tacgtttgat cacaattatc ttttatatta
    21541 aatgagaaac tataaaaaga ctccttaaag tttttaattt aaagagtctt tttttctttt
    21601 aattgtttat ggagagcctg acgtgcaaga ttatctgctc ctttattttt gttttcgggc
    21661 aaccattgca ggatcaataa agaaaattcg gatagaagtt cttggattct ttgaaggtag
    21721 ggaaggaaga gctcgttctt cgttgcgttt ttgtcgaccg tagaaactag tgttttgctg
    21781 tctgaaaaac agaaaatcgt ttcattctgt aagtcgtttt tctttagaaa ttctaaagtt
    21841 tttaaaaaga cttcgaactc tgcttgatgg tttgtcccta tagaaagcgg gatagatagc
    21901 tgttcttgtt taccttgata gacaatcaag attccacctc cactaggacc agggttgcct
    21961 tttgtagagg catcaatgta tacttttaac ataaaaagag attctccttt ttgttcaaac
    22021 taatgttatg gtaagattat atcattactg aatcggtgag aggttagaga atgaaaaaaa
    22081 atattcgatg gcagcctgaa ctggcacata cgatcatcta ttggtcattg accttgatca
    22141 ttctgttttt tagtctgatt ctttctttag aaaacacagg tccttattgg aaaagcaatt
    22201 ttgttttagg tatttttctt gtttttctat ttctaggttt gcggcgttct tttattttaa
    22261 aatcaaactc tgtgattata cgttatgctg ctttttggaa agaccgagaa atatctgtcg
    22321 gacagattcg atctttgacg ccaactaata ctggggtgca aatgatgttg caagatgaga
    22381 aagccccatc tgttttcatg atgcgaaaaa aagatcaagt agtccttttt gatcattttg
    22441 aaaaaaacgg acccaaatca gaatcaattc cttatcctta tacgaaaaaa agaaaaagct
    22501 aattttaaaa atcagaacct aaaaaaacga atttttacag gaaaagaaaa gagtgagacg
    22561 tacggttcat cccgtttagc gtctcactct atttgtttat ctattcatgc atactagtat
    22621 cggtagtctg aacgttgcct ttcttgacat gggcagcttg tgttcctcga ttcccttcga
    22681 tgatctcaaa ttcgaccact tgattttctt ccaaagtttt gaatccctct tcttcaatcg
    22741 cagtaaaatg tacaaaaatt tcttctgtgt catcataact gataaagccg tatccttttt
    22801 tgttgtcaaa ccattttaca attcctgtgg tcaaagcact cacgaccttt ctaaagtctg
    22861 atcatagcct tcattataaa gagtatttat aagtacgtca aataaattaa aaacctgtaa
    22921 ttttatgttt tttttctgaa taaaaaaagg tataattggc atatcttaac aaagaagata
    22981 ttttgttgaa taaagttcgt gaaaaggggt aatcaaatga agaatgatct gcaaatttca
    23041 caagaaacag agctagaacc gattgttgaa gtggctagta aattagatct taaggaagac
    23101 gatttggaac tgtatgggaa atataaggcg aaaatcaatt tttctggaat caatcgatca
    23161 aaggataacg ctgaggggca tttgatctta gttacttcca tcaatccaac accagctggc
    23221 gaaggaaagt caacgatcac tgtagggtta ggagacgcgc tcaataaaat caataaaaaa
    23281 tctgttattg cattgcgaga accctcactt ggtcctgtga tgggaatcaa aggtggtgct
    23341 gcaggcggag gatatgcaca ggtattgcct atggaagata tcaacttgca ttttactggt
    23401 gacatgcatg ctatcacaac agctaataat gcattatctg ctcttttaga taatcatatt
    23461 catcaaggaa atgaattgaa catcgatgcc cgccgagtga tttggaaacg ggtagtcgat
    23521 ctcaatgatc gtgaactgcg taaagtaatt gttggcttgg gtgggccaat ccaaggtgtt
    23581 ccaagagaag atgggtttga tatcacagtt gccagcgaaa tcatggctat cttgtgtctt
    23641 gcggcagacc ttgaagattt aaaggctcgt cttgcaagga tcgttgtcgg ctatacattt
    23701 gaccgtcagc ctgtgactgc tggagacttg aaagcagaag gcgctctggc attactgctg
    23761 aaagatgcaa tcaagccgaa tcttgtacaa acaatttacg gaacaccagc ttttgtccac
    23821 gggggaccgt ttgccaatat cgctcatggc tgcaatagta ttttagctac caagacagca
    23881 ttgagattag cagattacgt tgtcacagag gcaggatttg gtgcagatct tggcggagag
    23941 aaatttttag atatcaaagt acctaacttg aaaaaagcgc cggatgctgt ggtgattgtc
    24001 gccactgtcc gtgcattgaa aatgcatggc ggaatgaaaa aagatgaact taaaaatgag
    24061 aatcttgatg ctttgaaagt aggttttgca aatttaaagc gccatatacg taatatggaa
    24121 caatatcagc tacctgtgat cgtcgcaatc aatgaattcg taacagatac agacaatgaa
    24181 ctcacactgt tggagcactt gtgtgaaaat caggggattc ttgccaagag agcgagtgta
    24241 tgggcagatg gagcagaagg cggcgttgat ttagcaaaag ctgtagtacg attgattgat
    24301 agaaaagaag ctaattacaa gccattatac cgcctagaag aaacgataca agaaaagaca
    24361 gaaacaatcg tcaagaaaat ttacggagga aatggtgttg ttttcagcaa aaaagcgcaa
    24421 aaacaaatag aagagtttac gaagaatgga tgggacaaac tgccgatttg tatggcaaaa
    24481 acacagtatt cattttctga tgatccttca ctgctaggtg caccggaaga tttcaccatc
    24541 acgatccgcg aaatcatccc aaaattaggg gcaggatttt tagtagcctt gactggagat
    24601 gttatgacga tgcctggttt gcctaaaaaa cctgctgcat tgaatatgga cgtgacaaat
    24661 gacggagaag tgttgggatt gttttaagct tgattataat ctaccatcga aggagctgta
    24721 ttttgactac agcttctttt tttagacgat agggaattaa ttttgtttac actaggatca
    24781 ttctttttct ttattgtatt ttgagctata ctaaagcata ataggagggt atgatgaaaa
    24841 cgacatttat aagaaaaacc ggtttttacg gtatgggtag ctcgattcag ctaagaatca
    24901 acaatgaaaa acgcagtgtt ctcagtcatg aacaaaagat aacgcttgat ctggaagtgc
    24961 cctttacgat gcaggtcacc ttttattggc taaaaagccc cgtttataca gtgaaagaag
    25021 caaagcctgc ttatgtgatc acaatgaatt acttgattct tcaaatctat ccgtttttat
    25081 ttctagcaac tgggttgggg gccgttttcc tacaaaattt atttttcagt ttttttgcag
    25141 ttattgtgtt gattgcgttc tttttctata taaaaaaccg ggcatatttg atcaaggagg
    25201 aaaattatgg caaactctga tcttttttta atcagtttca atcgaatcga aaaatggatg
    25261 agagacgaga tgggaaatgc acgaaatatg ggatttaccg aacttgtcag acgcctagct
    25321 caaagaaaac aattaatgat cagaaaatac gaagatgatc ttttacagct tgctcagtta
    25381 aggaatgcga tcgtccatga tcggatcgca gtcgattttg tcattgcaga gccaaatgat
    25441 tggacagtca agcgaatcca gctgatcgaa caagaactga ttcagcctga aaccgtgctg
    25501 cctcgatttg caaaacgtgt aacaggtttt gaacaagatc ttccgttact tgaactgtta
    25561 aagatagtag cagaaaaaag gtattcacaa tttccgttat acaataaagg aaaatttgta
    25621 gcattgatta ctttacgtaa tatcgggttt tggctagcaa aagaaagcca aaaaggtccg
    25681 gttgatttaa caaataaacg agcgttggat ttgatcatcc aaaacggaaa gtacacaaat
    25741 tatcattttg ttccagctaa aacacatatt tacgaggttg aagcgatgtt tcgtgagcaa
    25801 gggactttgg aagccgtact tatcactaaa gatgggaatc ccgatggtaa tctgctcggg
    25861 atcgtacgac ctcgtgatat ttataaacaa atagaaaagg attgattttt tgttaattat
    25921 ttattggata ttcagcgctt tgctagtagg agtggatcag ttggtcaaat ggtggatcac
    25981 tgataatcta gcgcttggag agacgaaaaa tcttatacca ggtattttgt cactgaatca
    26041 tattcgaaac actggagccg catggagtat gctagaaggg aagatgtggt tctttactat
    26101 agtgacactc attgcagtcg ttgtgattct cacattgatg attaaaaatc gtgaaaaagg
    26161 taatcgctgg ttcatgattg gattgagttt gattctagct ggtgcgattg gcaattttat
    26221 cgaccgtatt cgtcttggct atgtagtaga tatgttccag acagatttta tgagctttcc
    26281 gatttttaac gtagcagatg tgacacttgt atgtggagtg atttgtatgt taatttatat
    26341 tattttagat gaaaaggatc aaagaaaaaa atgacagaac ttaaaacgac gattcaccat
    26401 gaatctggta ggatcgataa agtattgact aatttattca ccgattacag tcgttcgcaa
    26461 attcagctgt ggctgaaaaa tggagcggtt tctgtcaatg gacagccagt caaagcaaat
    26521 tacaaagtaa aacaaggaga cgagattact gtcttgatcc ccgaaccaga aacattagat
    26581 gtggtcccag aaaatatccc gttgacgatc gtttacgaag atgaagatgt agcggtgata
    26641 aacaaacctc aaggaatggt cgttcatcct tcagcaggtc atacgcatgg cacaatggtc
    26701 aatgcgctga tgtaccatat caaggatctt tctacgatca acggcgttat tcgtccaggt
    26761 attgttcacc gaatcgacaa agatacttcc ggactgctaa tggttgccaa aaatgaccag
    26821 gcacacgaat cattagctca gcaattaaaa gataagacct ctttgcgcaa atatgttgct
    26881 ttagtccatg gagagattcc tcacgaaaaa ggaaccatca atgcgccaat cggccgttcg
    26941 aaggaaaacc ggaaaatgca ggcgataaga gaagatggga agtcggctgt cacacatttt
    27001 actgtattgg aacgtttcga aggatttact ctggttgaat taaaactaga aaccggaaga
    27061 acacaccaga tccgtgtgca tatgaagtat atcggttatc cgttagctgg agatccagta
    27121 tacggaccga agaaaacttt gaaaggcaat ggtcagtttc tccatgcgca aatgttaggt
    27181 tttacgcacc cgactacaaa cgaaaagatg atctttgaag cacctcttcc agaagttttt
    27241 gaaaaaactt tagaaaaatt aagaaaaaac attgcatttt aatttagata acggtatagt
    27301 aattaacaac gaaagcataa aacttaatag aattccttta agtttagtcc cgtgaggcta
    27361 agaagggtgg gagcaaggat acgtatggtg cgccataagt agattgctca ttgacatagt
    27421 atagctatca gtgtatcagt atacgctcag tctaaacatt cctcctgtcc tcttagggca
    27481 ggagtttttt tatgcaaaaa agtaggggga atgaaaatgc aagcaaaaga agtagtagat
    27541 caggtaacca tgaagcgagc attgactcga attacttatg agattatcga gagaaaccat
    27601 agtatccaag atatcgtatt ggtaggtatc aaaacacgag gaatctacat tgcatcaaga
    27661 attgccgaac gcctgaagca gctagaagat atcgatattc ctgtaggaga actggatatc
    27721 actttatacc gagatgacaa aaaagaaaat ccagaagaac cggaacttca ttcttctgac
    27781 atcccagttt ccttagaagg aaaagaagtt attttgattg atgatgtttt gtatacagga
    27841 cgaacgattc gagcagcaat ggacgcagta atggattttg gcagaccaag aaaaatttca
    27901 ttagcagtcc tggtcgatcg tgggcatcga gaattgccaa tccgtgcaga ttatgttggg
    27961 aagaacattc caacatctcg ggcggaagag attttagtag agatgcaaga gcttgatgga
    28021 caagatcgca tcatgatttt gaaagaggaa gattagagga gagagaaaca tgtcggagaa
    28081 aaaaggattt cataatgatg acgtggtgtt agatatccac gatcgcccaa ccccaggaca
    28141 ttgggtagga ttaagtttac aacacttgtt cacgatgttc ggagcaaccg tgttagtccc
    28201 catcttagta ggtatcgatc caggtattgc gctagtcagt tcaggactag gtacattggt
    28261 gtacttattt actactaaag gaaagatacc tgcgtactta ggaagcagct tcgctttcat
    28321 tgcagcgatg cagcttttga tgaaaacaga tggttatcca gcgattgcac aaggagcagt
    28381 cacaactgga ttagtctatt tgattgttgc gctgatcatc aaaaagatcg gttcagcatg
    28441 gctggataaa atcttgcctc caatcgtcgt cggtccagtc atcatggtta tcgggttggg
    28501 acttgcggca aatgcagcaa caaatgctat gtataatcag ggcacgtatg atttcaagta
    28561 tattgctgta gcgctgatca cattaggatt aacaattttc ttcaacatgt ggctaagagg
    28621 ttttctcgga ttgattccga ttttactagg tattattagc ggatacatcg tagccttgct
    28681 gtttggaatc gtggatacta agccaatcgt agacgctgcc tggtttgctg ttcctaactt
    28741 tgagattccc tttgtacaat atgaaccaaa gctatatctt ggagcaatca caacaatggc
    28801 acccatcgct tttgtgacga tgacagagca tatcggccat cttatggtat tgaataaact
    28861 aactaaacgt aatttctttg aagaaccggg tcttcataag acgttgacag gtgatggatt
    28921 agcacaaatc gtggcagcct ttgtcggagg tcctcctgta acaagttacg gtgaaaacat
    28981 tggtgttctg gctatcacac gtgtccatag cgtgtttgtg atcggtggag ctgcagtatt
    29041 tgcagtcatt ttaggattcg taggaaaatt aagcgctttg attttaagta tccctggacc
    29101 agtcatctca ggaatcagtt ttatcttatt tggagtaatt gccgcaagtg gaatgaagat
    29161 tttaatcgag aacaaaatcg attttgataa aaagaaaaat ctgttgattg cttcagttat
    29221 cttagtagta ggaattggcg gactcgtctt tgaagtaggc acatttacat tgtcggcaat
    29281 ggcattagca actgtacttg gcatcatttt aaatcttatc ttaccagaaa cttcacgaag
    29341 cgaagaaaac taaggaggaa acattatgat catcaaatca gaacgtatca gcttaaagca
    29401 tttattgacc gttgaggcac ttactgatca agaagttatg ggcttgatcc gcagagggca
    29461 ggaattcaag caaggtgcta attggactcc gcaaaagcgc cagtactttg ctacaaacct
    29521 ctttttcgaa aatagtactc gcacacataa aagcttcgat gtagcggaga aaaaactagg
    29581 gctagaagtg attgaatttg aagcaagcac aagctctgtc caaaaagggg aaacactata
    29641 cgatacagta ctgacgatgt cagcattagg cgtagacgta gcggttatcc gtcatggtga
    29701 tgagaactat tatgacgaac tgatacaaag taaaacaatc cagtgttcga ttatcaatgg
    29761 aggagacgga agcggacagc atccaaccca atgcttgctt gatttgatga ccatttatga
    29821 agaatttggt cattttgaag gtctgaaagt tgccatcgta ggtgatatca ctcactctcg
    29881 cgtagctaaa tccaatatgc agatgttaaa acgtctaggc gcacaagtat tcttctcagg
    29941 tcctagagaa tggtacgacg atgaattcga agtgtacgga cattatatgc cattggatga
    30001 actactagat caagttgatg tcatgatgat gcttcgggtg caacacgaac gccatgatgg
    30061 aaaagaaagt ttctcaaaag aaggctacca tcaggaacat gggttaacag ttgagcgtgc
    30121 gaagaagttg caaaaacatg caatcattat gcacccagca ccagttaacc gtgatgtaga
    30181 attagctgat tcattagtgg aaggattaca gtcgcggatc gttcagcaaa tgagcaatgg
    30241 agtattcgtc agaatggcaa tcttagaagc agtgctaaat ggaaaagcgt aattatagga
    30301 ggatgaaaaa atgaaaacac tcattaaaaa cggtcagatc aatacacgaa aaaatatgac
    30361 gacaccagca gaaatttgga tagaagatgg gcgaatcaaa gcaatcggta caggattctc
    30421 agaagccgaa tttgatgaag tgtttgatgc aaagggacaa ttgatcacac ctggtttagt
    30481 ggacgtccat gttcatttga gagagccagg atttacatac aaggagacga tcgaagctgg
    30541 gactcgctca gctgctcgtg gcggtttcac tactgtatgc gcgatgccga atttaaatcc
    30601 tgtacctgat acggcagaaa agctgagaca agtatatgac atcattagaa aagatgcagt
    30661 tgtgaaggta ttgcagtatg cgccaatcac agaaaatctg cgaagtgaga aattagttga
    30721 tcaagaagca ctgattgaag aaggtgcttt tgctttcaca aatgacggtg taggtgtcca
    30781 aacagctggg acgatgtatc tggctatgaa agaagctgca aaaaacaata aagccctagt
    30841 agcccataca gaagatgaat ctttattatt tggcggcgtt atgcacgctg gcaagaaggc
    30901 agaagaactt ggtcttcccg ggatcttaag cgtaaccgag tcttctcaaa ttgctagaga
    30961 tctgttgcta gctgaagcaa ctggtgttca ttaccatgtt tgccatgttt ctactaaaga
    31021 aagtgtccgt gtaatcagag acgcaaaaaa agcaggaatc cacgtaacag cagaagtttc
    31081 accccatcat cttattttga tcgacgaaga tattcccgaa gatttcggct tttggaaaat
    31141 gaacccgccg ctaagaggac gggaggacag agaagctttg atcgaaggct tgcttgacgg
    31201 tacgatcgat tgcatcgcta cagaccatgc cccccatgga ttagaagaaa aaagccaaag
    31261 tttcatgaaa tcaccatttg gtatcgtagg aagcgaaaca gcgtttcaac tgatctatac
    31321 tcattttgta gaaactggtc gattcacgtt ggaacaagtg atcaattggt tggcagttaa
    31381 accggctgag atttttggtt tgaatgcagg aacgctaacg gttggtgccc ccgctgatgt
    31441 agcagttttt gatattacac aaacttgcac gatcgataaa gaagactttt tatcaaaagg
    31501 ggagaataca ccttttatcg gctggaaagt aaaaggcgaa acacaaatga cttttgtcaa
    31561 cggaaaactt gtgtggcaga agggagaata aaagtatgga acgtttgctt atattagaag
    31621 atggaacagt tttcgaagga aaggcttttg gtgcagatgc tagtgtcttc ggagaaatcg
    31681 tttttacaac aagtatgact ggttatcaag aaactatcac tgatcaaagt tttaacggtc
    31741 aaatcattac atttacctat cctatggtgg gtaattatgg ggtaaataga gatgattatg
    31801 aatcgatttc tccaacatgc aaaggagtag tagtaaaaga acatgcacgt gtagcttcaa
    31861 actggcgaaa ccaaatgacg ctagattcat ttttgaaaca aaaaggaatt cctggaatat
    31921 caggtatcga tacgcgcgcc cttactcgca aaatccgtga agtcggaacg atgaaagcta
    31981 gtatagttga tgctggagat tccttcgaac atgcatttga tcagctgaaa gcaaccgttc
    32041 tagcaaccaa tcaagtgcag caagtctcaa ctaacaaacc ttatccaagt ccaggaacag
    32101 gtcggaaagt cattgtcatt gacttcggct tgaaacatag tattttacga gaattgagta
    32161 aaagaaactg taacctcaca gtacttccct ataacacaga tgccgagacg attttagcgc
    32221 tatcaccaga cggagtcatg ctgacgaatg gacctggtga tccaaaagat gtgccagaag
    32281 caatcgaaat gatcaaaaaa atccaaggaa aagtaccgat ttttggtatt tgtctcggtc
    32341 accagttatt tgctttagcc aacggtgcag atacctataa aatgaaattc ggacatcgag
    32401 gattaaatca cccggtacga gaagtagcga ccggaagaat tgattttact tctcaaaatc
    32461 atggctatgc agtcgatgaa gccacgattg atccagaaaa actcatcgta actcatgtag
    32521 aagtaaatga cggaacagta gaaggagtca aacacagaga ttatcccgct ttcagtgtgc
    32581 agtttcatcc agatgctgcc cctggtccgc atgatgcact acatttattt gatgaattta
    32641 tggaaatgat ggacgcagga aaggagcagt aaacagatgc cgaaacgtac agatatcaag
    32701 aaaattatgg tgattggatc tggtccgatc atcattggtc aagccgcaga atttgactac
    32761 gcaggaacac aagcctgcct tgccttaaaa gaagaaggat atgaagtggt tttagtaaat
    32821 tctaatcctg cgacgatcat gacggataaa gaagtggcag atcaagtata tatcgaaccg
    32881 atcacacttg aatttgtatc aaggattctt cgaaaagagc gtccagatgc tctccttcca
    32941 actttaggcg gacagacagg cctcaatatg gcaatggagc tggcagcatc aggtatttta
    33001 gaagaattga acatagaact tctaggaaca aagctttctg cgatcgatca agcagaagat
    33061 cgtgatctat tcaaacagtt gatgaaagaa ttgaatcagc caatccccga gtctgaaatc
    33121 gtcaatacag tagaagaagc tgtagatttt gcaaataaaa tcggctatcc tgtcatcgtt
    33181 cgtccagcct ttactttagg cggaactggt ggaggtatgt gtgaaaatga agaagaatta
    33241 cgtatcgtat cagaaaatgg attaagtctt tcccccgcta cccaatgttt gattgaacga
    33301 agtattgcag gttttaaaga aattgaatat gaagtgatga gagattcggc tgataatgca
    33361 atcgttgtct gcaatatgga aaattttgac ccggtgggta tccatacagg tgattctatc
    33421 gtatttgccc ctagtcaaac attgtctgac catgaatatc aaatgcttcg tgatgcttct
    33481 ctttcgatta ttcgagcact taagatcgag ggaggctgta atgtccaact cgctcttgat
    33541 ccacatagtt ttaattatta tgtgattgaa gtcaatccgc gtgtttctcg ttcatctgcg
    33601 ttagcaagta aggcgactgg ctatccgatc gctaagctag ctgctaaaat agcgcttgga
    33661 ttaacattag acgaaatgaa aaatcctgtt acaggaacga cttttgcaga atttgaacct
    33721 gcattagatt atgttgtagc taaaattcct cgctggccgt ttgataagtt tgaaaacgga
    33781 gaacggcttt taggcacgca gatgaaagca actggtgaag tgatggctat cggtcggaat
    33841 atcgaagagt ctttattgaa agctgtgcgt tctttagaaa tcggtacaca ccatatcgaa
    33901 cttcctgagt tggcagacat cacggacgat gacctaatcc aaaaagtggt tcgagcacaa
    33961 gatgatcgcc tgttttatgt agctgaagct attagaagag ggtacaccat agaagaatta
    34021 gcggatttga caaaaatcga tttattcttt ttagataaac tgttgcatat cgtagaaatc
    34081 gaacagcagt tgaaacagca gccgttggat gacaagatat tgaaaacagc gaaacaaaac
    34141 ggttttacag acaaaaaagt ggcggagctg tgggaaataa acgaagaagc cgttcgccag
    34201 atgagaaaag aaaatgattt gattcctgta tataagatgg ttgacacttg tgcagcggaa
    34261 ttcgaatcaa aaacacctta tttttatagt tcctatgaat atgaaaatga aagttcacga
    34321 tcagaaaagc cctcaattct agtactggga tctggtccta tccgtatcgg acaaggggtt
    34381 gaatttgact atgcgactgt gcattctgtc aaagcaatcc agcaggcagg atacgaagca
    34441 atcatcatga acagcaatcc tgaaacagtt tcgactgact tttctatttc ggataaatta
    34501 tattttgagc cattgacctt cgaagatgtg atgaatgtga tcgagctaga acagccgata
    34561 ggagtaatcg ttcaatttgg tggtcagaca gcaatcaact tagccgaacc tttgactgct
    34621 gctggtgtaa aaatcttagg gacaacgatc gaagacttgg atcgtgcgga aaatcgtgat
    34681 ttattcgagc aagcgctgaa agagttggat attcctcaac ctttagggga tactgctaca
    34741 agtaaagaag aagccgtggt tatcgcttcg agaattggtt atccagttct tgttcgtcca
    34801 agctatgtat taggcggacg tgcgatggaa atcgttgaaa accagcgaga tttagaagat
    34861 tacatggaac atgctgtgaa agcatctcca gaacatccag tactagtgga ccgttacttg
    34921 cttggtggtg aatgcgaagt ggatgcgatt tgtgacggtg aaacagtact catcccaggg
    34981 atcatggagc atattgagcg tgctggtgtc cattcagggg attcaatggc tgtgtatcca
    35041 cctcaaacat tatctgaatc gatcaagaaa acgatcgaag attacacgat ccgcttagca
    35101 ttaggtttga attgtgtcgg gatgatgaat atccagtttg tcatccatga aaatgaggtt
    35161 tatgtgatcg aagtcaatcc tcgagcaagt cggactgttc cattcttaag taaggtgaca
    35221 ggaattccaa tggcacaaat tgcgacgaaa gctattttgg gagagaaact aaagacactt
    35281 ggttatgaaa acggacttta tccagaaaca aaagaagttc atgttaaagc acctgtcttt
    35341 tcttttacga aattgcagaa agttgatact tacttagggc ctgaaatgaa atcgactgga
    35401 gaagtaatgg gatctgacag acaattagac aaagctttgt acaaggcatt cgaagcttct
    35461 ggattacact tgcctgaata tggagccgta ttatttacga ttgcggacga aacaaaagaa
    35521 gaagcactag aattagctaa acgttttaca gaaattggct atagtctgat aggaacgaaa
    35581 catacagctg cttatttcga aaaaaatggt ctggtcgttg aaacagtagc gaaaatttct
    35641 gaggaagcag cagaaaaaaa tgtagtcgac ttaataagag atggtaaaac acaagtggtt
    35701 gtcaatacga ttgataaaga tcggggaaat gcttctaaag acggtttcat catccgaaga
    35761 gaagcagttg aacatggtat tccattgttt acttctttag acacagcaga tgcgatcgtt
    35821 cgagtgatgg aatcccgtgc tttttcaact aaccctattt aacggcgagc acatgttaag
    35881 acaaaaagac gaaaaaccgt tgcaggaggg gacagatgaa acaagaaatg atggaaatcg
    35941 ttcagcaaaa acagctggca ccaaagattt ttgaaatgac attgaaaggt gatctagtaa
    36001 aagaaatgca gcaaccagga cagttcttgc atattctcgt gccgcgtgca gacttgctat
    36061 tacgccgacc gattagcttg aatcatatcg accacgaaac aaatacttgc cgtatcattt
    36121 atcgggtgga aggtgagggg actagagtct tctctactct ttctgccgga gaccatttag
    36181 atgtcatggg tcctttaggc aatggttttg atttgtccaa tcttcataca ggagacgaag
    36241 tatttataat cggtggagga atcggtgtcc ctcctctata tgagttatca aaacagttag
    36301 tcgaaaaagg aattaagcca acgcattttt taggttttgc cacacatgaa gtcgtttatt
    36361 atgaaaaaga atttaatcgt ctaggagaaa caagaatcgc tactgacgat ggaacatacg
    36421 gtatgcacgg aaatgtcggt aatttattgt taggaacgaa gacggaacct acagctgttt
    36481 ttgcatgtgg ttcaaacggg ttattgaaga cagtggaaca gctgttttct tcgcatcaaa
    36541 atgtccagtt atcattagaa tcccgcatgg catgtgggat aggggcttgc tatgcttgcg
    36601 tttgtcatac gccggaagat gaaacaggaa caaaaagtgt gaaagtttgt gacgaaggac
    36661 cgatttttaa aataggagag gtaattatat gaatgagaat cctttagcgg tcaaacttcc
    36721 tggcctagaa ttaaaaaatc ctatcatgcc ggcaagcggc tgtttcggtt ttggcaaaga
    36781 atacggaaaa tactatgatt tgaatcagct tggatcgatc atggtcaagg caacgactcc
    36841 aaatgcccgc tttggcaatc ccacgccaag agtggcggaa acaccatctg gcatgttgaa
    36901 tgcaattggt ttgcaaaatc ctggtctaga cgttgttatg cataaacttc ttccagaatt
    36961 agaggagtac aatgagttac ccatcatcgc aaatgttgct ggtgcttgtg aagaagacta
    37021 tgtagaagtt tgtagcaaaa ttggcgaagc accaaatgtg aaagcaatcg agctgaacat
    37081 ttcttgccca aatgtcaaac atggcggaat tgcttttgga acagattctg atgttgcttt
    37141 tcaattaaca caagcagtaa agaaagtttc ttctgttcct gtctatgtca aattgtcccc
    37201 aaatgtaaca gacatcgttc caatcgctca ggcaatcgaa gctggagggg cagatgggtt
    37261 tacgatgatc aatactcttc ttggtatgcg gattgacctt aagacaagaa aaccgatttt
    37321 agccaatcaa acaggtggac tctccggacc agcaatcaag cctgtcgcta ttcgtttgat
    37381 tcaccaagta gcaagtatat ccagccttcc aatcattggg atgggcggcg tgcagacagt
    37441 ggatgatgtt cttgagatgt ttatggctgg agcaagtgct gtagctatag ggaccgcgaa
    37501 tttcaccgat ccgtatatct gtcctaaact aattaaagag ctccctgtgc gaatgagcga
    37561 actaggcatc gaatcgctgg aaagattaat caaagaagtg agagaggaac gtgaacgatg
    37621 aatcaactag accaacgacc aattattgca ttagattttt caactaggca agaggtagaa
    37681 gactttcttc gatttttccc gaaagaagaa aaattgtttg tgaaaatcgg aatggaattg
    37741 ttctaccaag aaggacctga aatcgtacgt tatctaaaag aggctggtca taatgtattt
    37801 ttagatctga aacttcacga tattccgaat acggtcgaaa aagcaatgcg gggattggca
    37861 aagcttggtg ttgatctcac ctgtgttcat gccgctggcg gtattcgtat gatggaggcg
    37921 gcaatgcgag gattagaaga ggggactcca gaaggtggaa aacgtccgct acttctagct
    37981 atcactcagc ttacttctac aagtgaagaa gaaatgcatg ccgatcaact aatcgaagtt
    38041 tctttagaaa aaagtgtgat ccattatgct agctgtgcca aaaaagcagg attagatggt
    38101 gtggtttctt ctgcttggga agtagaagcg atcaaagaaa tggcaggaga cgaatttgtt
    38161 tgcttaactc cgggtataag accagaagga acagtcgcag gagatcagac aagagtagtc
    38221 acaccttctc aggcaaaaaa gatcggctca acttttatcg ttataggtcg accaatcact
    38281 cagtcaacta atccttataa agcatatcaa acgattcaaa cagaatggag ctaacctaaa
    38341 atgaatgttg aacaaagtat cgcaaaagac ttattagaaa ttgaagcagt atttttaaat
    38401 ccaagtaatc cttttacatg ggcaagtgga atcaaaagcc caatttactg tgataaccga
    38461 atcacgatga gctatccaaa agtccgaaaa gagatcgcaa aaggattagc aagcaaaatc
    38521 aaagaagctt accctgaagt acaagtgatt gctggaactg caacagcagg aatccctcat
    38581 gccgcatggg tagcggaaat actggatttg ccaatggtct atattcgaag taaggcgaaa
    38641 gatcatggga aaggtaacca aatcgaagga cgtatatttg aaggtcagaa gatggttgtg
    38701 atcgaggatt tgatttctac aggcggaagt gtcttggaag cagctgaagc agcaaaaaga
    38761 gaaggtgcgg atatccttgg tgttgcggct atctttacgt atgagttgcc aaaaggaaaa
    38821 gcaaatttcg aaaaagccga aatccctctt atgacgttga ccaattattc tgtattgatt
    38881 gaagcagctt tagaagatcg ttatatcgat gagcaagaat taactctgct aaaagaatgg
    38941 aaaaaagatc ctgaaaattg gcagatttaa gcgcgcttga tgaagataga gaaatccgat
    39001 ttgtgtctcc agaatcatca attggaatag caaaaatcca aaaactgatt gcgagaagca
    39061 tcttttcatc aggtatgaaa agaaacttct atcgtgatca gtttttgatt tttttaaaat
    39121 tcatttttct aagactggtt agcagaaaga agagaataat c
//
LOCUS       DACECZ010000012        35603 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.012, whole genome shotgun sequence.
ACCESSION   DACECZ010000012 DACECZ010000000
VERSION     DACECZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.012"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            285..2051
                     /locus_tag="IWO35_000650"
     CDS             285..2051
                     /locus_tag="IWO35_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ0315727.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2153..3412
                     /locus_tag="IWO35_000651"
     CDS             2153..3412
                     /locus_tag="IWO35_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0315728.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3559..4860
                     /locus_tag="IWO35_000652"
     CDS             3559..4860
                     /locus_tag="IWO35_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0315729.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4863..5714
                     /locus_tag="IWO35_000653"
     CDS             4863..5714
                     /locus_tag="IWO35_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0315730.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5727..6542
                     /locus_tag="IWO35_000654"
     CDS             5727..6542
                     /locus_tag="IWO35_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ0315731.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6693..7859
                     /locus_tag="IWO35_000655"
     CDS             6693..7859
                     /locus_tag="IWO35_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0315732.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8089..8736
                     /locus_tag="IWO35_000656"
     CDS             8089..8736
                     /locus_tag="IWO35_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ0315733.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8820..9764
                     /locus_tag="IWO35_000657"
     CDS             8820..9764
                     /locus_tag="IWO35_000657"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ0315734.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9899..11350
                     /locus_tag="IWO35_000658"
     CDS             9899..11350
                     /locus_tag="IWO35_000658"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ0315735.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11371..12207
                     /locus_tag="IWO35_000659"
     CDS             11371..12207
                     /locus_tag="IWO35_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0315736.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12207..12866
                     /locus_tag="IWO35_000660"
     CDS             12207..12866
                     /locus_tag="IWO35_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0315737.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            12992..13681
                     /locus_tag="IWO35_000661"
     CDS             12992..13681
                     /locus_tag="IWO35_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315738.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13687..14643
                     /locus_tag="IWO35_000662"
     CDS             13687..14643
                     /locus_tag="IWO35_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ0315739.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14687..15199
                     /locus_tag="IWO35_000663"
     CDS             14687..15199
                     /locus_tag="IWO35_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0315740.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15455..16003)
                     /locus_tag="IWO35_000664"
     CDS             complement(15455..16003)
                     /locus_tag="IWO35_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0315741.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16092..17273)
                     /locus_tag="IWO35_000665"
     CDS             complement(16092..17273)
                     /locus_tag="IWO35_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ0315742.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17509..19017)
                     /locus_tag="IWO35_000666"
     CDS             complement(17509..19017)
                     /locus_tag="IWO35_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ0315743.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19176..19367
                     /locus_tag="IWO35_000667"
     CDS             19176..19367
                     /locus_tag="IWO35_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315744.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19570..20487
                     /locus_tag="IWO35_000668"
     CDS             19570..20487
                     /locus_tag="IWO35_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ0315745.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20480..21553
                     /locus_tag="IWO35_000669"
     CDS             20480..21553
                     /locus_tag="IWO35_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315746.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21701..22219
                     /locus_tag="IWO35_000670"
     CDS             21701..22219
                     /locus_tag="IWO35_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315747.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22200..22592
                     /locus_tag="IWO35_000671"
     CDS             22200..22592
                     /locus_tag="IWO35_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ0315748.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22668..23603
                     /locus_tag="IWO35_000672"
     CDS             22668..23603
                     /locus_tag="IWO35_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ0315749.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23736..24515
                     /locus_tag="IWO35_000673"
     CDS             23736..24515
                     /locus_tag="IWO35_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ0315750.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24508..24825
                     /locus_tag="IWO35_000674"
     CDS             24508..24825
                     /locus_tag="IWO35_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0315751.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            25069..26679
                     /locus_tag="IWO35_000675"
     CDS             25069..26679
                     /locus_tag="IWO35_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0315752.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26836..27684
                     /locus_tag="IWO35_000676"
     CDS             26836..27684
                     /locus_tag="IWO35_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0315753.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            27915..28394
                     /locus_tag="IWO35_000677"
     CDS             27915..28394
                     /locus_tag="IWO35_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315754.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNVLNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            28427..30655
                     /locus_tag="IWO35_000678"
     CDS             28427..30655
                     /locus_tag="IWO35_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0315755.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            30784..32427
                     /locus_tag="IWO35_000679"
     CDS             30784..32427
                     /locus_tag="IWO35_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0315756.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(32500..33045)
                     /locus_tag="IWO35_000680"
     CDS             complement(32500..33045)
                     /locus_tag="IWO35_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ0315757.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(33068..33553)
                     /locus_tag="IWO35_000681"
     CDS             complement(33068..33553)
                     /locus_tag="IWO35_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0315758.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(33543..34355)
                     /locus_tag="IWO35_000682"
     CDS             complement(33543..34355)
                     /locus_tag="IWO35_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ0315759.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(34494..>35603)
                     /locus_tag="IWO35_000683"
     CDS             complement(34494..>35603)
                     /locus_tag="IWO35_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ0315760.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        11649 a         6404 c         7115 g        10435 t
ORIGIN      
        1 ttatgtttca ttcttctaga atatacttct acgccaaaaa ctgttaaaaa acttttactt
       61 tagaaaattg gagaaattga tcgcttaaga taaattcagg acactaaata gtacaggaag
      121 tagactaaaa cttttaaaag ttttaagtct atctatttgg caagtttaaa aaataaaaat
      181 tcggtatcaa aaaactgtta ccgataacat ttttgaaagc gttgtctttt gcttctctat
      241 cacttatagt gatgacgtca tattaaaaca caaggaggaa atcaatggat acagctgcaa
      301 tttatcatcg accggaaagt gaatatgcat ttctatatac gaatacaaga tttcgtattc
      361 ggcttcgtac taggaaaaat gatatcaaaa aagtatatgt gctatgtgga gatccatata
      421 ccataacaac tgaaaaatgg tatcaaaaac aacgaccaat gaaaaaatgt ctaagtacca
      481 atgttcatga ttattgggaa attgaagtga cgagcgaaac aagtcgctta cagtatgcgt
      541 tccatgttgt tggggaagat aacacggact gtttttacgg tgatcaagga atttttcctt
      601 atcgagaaaa tgtgattact gaaccaaatt tttattttcg aataccttat tttcatcaaa
      661 tcgatcgatt cactattcca gaatgggtaa aagaaacagt atggtatcaa atttttcccg
      721 aaagatttgc aaacggggac aaagcaaatg accccgaaca cactttacct tggggaagta
      781 aagatccaga ccgagaagac tttttcgggg gagatttgca aggtgtgatg gatcatttgg
      841 attacttgac ggaccttggc atcaatggca tctatttttg tcccatattt aaagccacat
      901 ccaatcataa atacgatacg attgattatt atgaggtgga tcccgctttt ggcgataaaa
      961 aacttttaaa aaacttgatt gatgaagcac ataaaagagg gatacgtatc atgttcgatg
     1021 ctgtattcaa tcatatgggt gtccattcac cacagtggca agacgttctc aaaaacggag
     1081 aaaaatcgat ctataaagac tggttccaca tccgtttttt cccagtagat tcttatcaga
     1141 tgactgactt accagaaact gctgaaaata tcccatatga tacttttgct tttactccat
     1201 tcatgccgaa actaaataca gcaaatccag aagtgcaaaa atatttgctg gatatcgcaa
     1261 cttattggat aaaagaattc gatattgatg gatggcgtct tgatgttgct aatgaagtcg
     1321 atcatcattt ttggaaaaaa ttcagagaag cagttacaga aatcaaacca gacatctata
     1381 tccttggaga gatttggcat tcctctcaag cttggctgca aggggatgaa ttccatgcag
     1441 tgatgaatta tgcatttaca gattcgatta aagattattt tgcaaaaaag aaaatcacag
     1501 cttcacaaat ggtcagtgga atgaatcatc agcaaatgct ttacagagat caggtgaacg
     1561 aaggaacatt caacttatta gattctcatg atactgccag gatcttgacc ctttgccaag
     1621 gtaacaaaga attgatgaag tctgtgatgg cctttatgtt tcttcaaaaa gggtctcctt
     1681 gtatctatta cgggacagaa atagggatga ctggtgaaga tgacccggat tgccgcaaat
     1741 gtatgatatg ggagaaggag gaacaagatc tagagctatt cggttttata aaagaacttg
     1801 tttctctaag gaaacagttg tctaagatta tttctgaagg ttctactcag tggctaatag
     1861 taaatgatcg tgaagataag ctctatttca cacggaaact agagggacag atcatctatg
     1921 tttactttaa tcagtcaaaa gaaccttggg tagtagaaca ggaaaatgag gtgattctta
     1981 gtcaaaattg tcagttgtta gaagacggga aagcagagat acgacaatac ggatttttga
     2041 tcatgtgttg aaaactgtta ccggtaacaa caagaaagcg tttgcaaatg aagatttgtg
     2101 cccattataa taagggtgta acagcacgtt gaatacaaag gaggaaatga agatgaaaaa
     2161 aagaagtgtg aaacagttgg gtttagggtt aatctcttta ggaatcttag gcggattagc
     2221 ggcttgtggc aatggtaact cttcagcaga aggggaagcc gatgaaggaa aaacgttaac
     2281 agtttccgtt gattctggat ataaagatta tataaacgaa ataaaaaaca ctttcgaaaa
     2341 agaaaacgat gtgaaaatca agctagtaga aaaggacatg ttcgatcagt tggaatcatt
     2401 agcgttagat gggcctgctg gaaagggacc tgacgttatg atggcagcat atgaccggat
     2461 cggtgcgtta ggacagcaag gacatctggc ggaagtaaaa ctgggaaatg aagcagctta
     2521 cgatgaaaca gacaaagccc aagtgactta tgatggaaaa atctacggag aacctgcagt
     2581 aatcgagacc cttgttttgt actataacaa agatttagtc gatacagcac catcaacgtt
     2641 taaagatttg gagaatctat ccaaagacag tcgttttgct tttgagtcag aagcaggaaa
     2701 aaatacagga tttttagcta aatggactga cttttattat tcctatggtt taattgcagg
     2761 ttacggtggc tatgtatttg gtgatgatgg cacaaatcct tcagatatcg gtttgaataa
     2821 tgcaggtgct gtagaaggaa tctcttatgc aacagattgg ttcaaaaatg tttggccaaa
     2881 gggaatgcaa gacataaaaa gtgcaggaga cttcgccagc cagcaattca tgtccaataa
     2941 aactgcagca atcattgacg gtccatggca agcgcaaaca tacaaagaaa acaatatcaa
     3001 ttatggtgta gccaaaattc ctactctaaa taatggacag ccttatcaac cgtttggcgg
     3061 aggaaaagga tgggtagtca gcaattatgc taaaaacaaa gacctctctc aaaaatggct
     3121 tgattatgta acgaatcagg aaaatcaaga aaaattcttt gaaatggtaa atgaaatccc
     3181 tgcaaaccaa caagctaggg aaacagcaaa ggctaaaaac gatgaattaa caacagctgt
     3241 gattgaacaa tatgaaacag ctcaagcgat gccaaacatc ccagaaatgg gcgaagtttg
     3301 gacaggtgca gaaaacttga tgttcgatgc agcatcaggt tcaaaaacac caaaagaatc
     3361 cgcagatgaa gcagtaaaaa caatcagtga agccatagaa caaaaatact gattaaaatg
     3421 ataaccgtcc atttttataa aaaatggaac aggtgcttca gataaaagtg gcaagagcga
     3481 gatggaaaat tatcttgctc ttgttgccat tatcttaaaa gcagtttgcg gtttagcaag
     3541 tttagagggg gcattcagat gcagcaagat aaaacaagca atatcaacga aaaaaaaatc
     3601 acgctttgtt cacttattcc agggatgggg cagtttgtaa atggccaagc ttttaaaggt
     3661 atcgtttttt tcgccatatt cgtcctattc attggtcaga tgcttctggg cggatttcat
     3721 gcattggtag gattggttac tttgggaagt ataccaatgg aagatcattc attatttatt
     3781 ttgattgaag gcactttgca attcatcatt acaatcattt tcttgatttt ttacggtttg
     3841 aatcttttag acgcttatcg agtagcgaag atgcgtcaag agggcaaaaa aatcaatcgg
     3901 acgatgaagg aagtcataca taatataggt gatgaaggtt ttccttatct attgacactt
     3961 cccgcttatc ttctcatgat ttttacgatc attttcccag tcctagttac attgttcact
     4021 gcatttacta attatgactt caatcatatt ccaccagcca gtttaattga ttgggtaggg
     4081 gctaaaaact tttttagtat cttcttttta agctcatatc gtaatacttt ttttgcagtc
     4141 ttttcgtgga ccgtgatttg gactttatgt gcaacaactt tgcaaatcac ccttggtatt
     4201 ctcacagcgg ttgttgctca ccagaaattt attaaattca aacgtatttt cggtatcatt
     4261 ttcttattac catgggcggt cccagctttt attactatca tgagtttttc gaatatattc
     4321 aacgatagcg taggtgccat caatactcaa gtgattcctt tcataaacca gcttcctttt
     4381 gtagatatca gtgcaatctc ttggaaaacg gacccgaact ggacgaaagt agcaattatc
     4441 ttagtgcagg gctggttagg atttccctat atctatgtga tggttacagg aatcttacaa
     4501 tcgatttcag aagacttata tgaagcagca aaaatcgacg gagcaaatgc atggcagcgc
     4561 ttttcaaaca tcacgcttcc tgctgtcttc ctagtggcag cgccaacatt tattacacag
     4621 tatacgggga atttcaataa tttctccatg atctatctgt ttaacaatgg cggtcccgga
     4681 agcgtaggcg gaggagctgg ttctactgat attcttattt cctggatcta taaacttacg
     4741 actggaactt ctccgcagta ttctatggct tcagcaatca cgttgattat ctccgtcatc
     4801 gttatctgtg tgtcattgct ggtatttaaa aagacaaatg cgttcaatat gggggattaa
     4861 caatgaaaaa atttcattcg agtgctcgat tccaaaaacg actttctcac ttttttactt
     4921 acttttttct tttaatactg tcagtcatta tcatctatcc gcttttgatc acagcaagta
     4981 ctgccttcaa gtcaggaaat attgcagctt ttagtttaga tctttcaagt gactggactt
     5041 taggaaactt tactcggcta ttcagtgaaa cactttacgg gacttggtat aaaaatacat
     5101 tgatcatttc tgtcctaaca atgatcgtac aagtgactgt agtaactttg gcgggctata
     5161 cttatagtcg ctatcgtttt atcggccgca aatcaagttt gatgttcttt ttagtgattc
     5221 agatggtacc gactatggca gcattgactg ccttttatgt catggctttc ttattagaag
     5281 cgctcgataa gtactggttc ttgacaatga tctatatagg cggtggaatt ccaatgaata
     5341 cttggctgat gaaaggatac tttgacactg taccaataga tttagatgaa tctgctaaac
     5401 ttgatggggc agggcatttc aggatttttt tccagattgt attaccgctt gtcaaaccga
     5461 tgctcgccgt tcaagcatta tgggctttta tgggaccttt tggggatttc atgttagcta
     5521 agttcctgct tcgaacacct gaaaatcaga ctgttgctgt cggcttacaa acatttattt
     5581 ctgatgtgac aaaccaaaga gtctctcttt ttgctgctgg ggcaattttg attgcactac
     5641 cgatttgtct attattcttc tttttacaaa agaactttgt taccggcctg acatctggcg
     5701 gaacaaaagg ataggagcag agtatcatgc aaacatttcc gttaaactat ttttccgcat
     5761 tacgtacgcc aactcaagtg tttgcgggac gcaaactatt atcatggcct aaatttttct
     5821 tgatatttgt atttttagtt tccttgatgg tcatgcctgt gactcttttt tatgccaatc
     5881 agatccaagc gattccatta gaacaatttc tatccgttca ttcgctgatc gatgaacaag
     5941 gaacccaaaa attttctgaa cttgaattat ctgaaactgg gttacaagca tcgcaacaaa
     6001 cgatagctgt gacccctgaa attttggtag gtgtgtcttt atctgagaaa caacaatccg
     6061 atcatggaac ttttattgat tttgagaagg aacaatgggt gatccaacaa aaggataaat
     6121 ctggtatacg ccgctatacg atgaattata gcccatcttt ccaaccagat tctgtacgta
     6181 cgcccgaaga tttccaacga tttttagaaa gagaatttta tgccagtaat cgtccaacaa
     6241 tcattttatc ttatagttta agtcttggtc ttgtcctatt tgtcatgacg agtctcattt
     6301 tattcggggc ttcctttttc ttgtggatga caagaaaaag ccaactttca tccattcata
     6361 cattcaaaga gagtgcaaat ttgatgctga atgttatggg aataggttcc atggcagcag
     6421 cagttgtagg gtttatccac tttgacttta tcttgatgct aagtgtccaa acaacgataa
     6481 ctgtcttgct gcttttatgg gtttttgtca aaacaagatt caaagataag cgtgtcgaat
     6541 gacaagttta agcgatttat gcgacaatga atgtgaaaaa aatgaaaaat aaagtagttt
     6601 ttgaaaaatt tctgaaaaaa ttaaacttct tagttgattc ttttcttttt tagtattaac
     6661 gtaatagggt agttttttta aggaggaaaa tcatgttaga gaaaacttta tacaatcaat
     6721 tattttcacg ttctttctcg ctccctgtcg aagtaaatta ttgggacggt acaacgaaac
     6781 aatacggaga tacggacact cctccccaaa tcaaaatcaa tatccatgaa gaaatcccaa
     6841 tgaaagagat tacaagtaac gcatctttgg cactaggaga agcctatatg gatcatcgaa
     6901 tcgagatcga gggaagtatc caagcattga tcaacgatgc ttatcagcaa gcaaacagct
     6961 ttttacgagg aaaagattat ctaaaatggc tgccaaagaa agaaaaacat acgaaacaaa
     7021 aaaacaaaga agatatccat gcgcattatg acttaggtaa cgacttttat aaactttggc
     7081 tagatccaac tttgacttat tcttgtgctt atttcaatac acctgaagat acgttggaac
     7141 aagcacaaat caataaagtc catcatatct tagacaaact ctttatcaaa gaaggcgaaa
     7201 cgttgcttga tatcggttgt ggctggggaa cattgatgtt cactgctacc aaagagtata
     7261 atgtcaaggc aactgggatc acactaagtg aagaacaata cgatttgatt accaaaagaa
     7321 taaaagaaga acatttagaa gataaatgcc gtgtattatt aatggattac cgcgaactaa
     7381 aaggcgaaac gtttgatcat atcacaagtg taggtatgtt cgaacatgta ggttctgaaa
     7441 atcttgaagg ttatttcaaa gtggtaaaag atttattgaa accaaaagga acagctttga
     7501 tccatggaat cagccgacaa caaggcggtg cgacaaatgc ttggatcaat aaatatattt
     7561 tccctggcgg ttatgtccct ggtgttactg aattagttga taatattaca aaaaacgatt
     7621 tgcaactgat tgatttggaa agcttacgtc gtgactacca attgacatta gaacattgga
     7681 caaagaattt tcatcaagtc caagatcaag tcacgaaaga aatgggtgat tccttctatc
     7741 gaatgtggga tctatattta caagcttgtg cagcttcatt tgaatcaagt aatatcgatg
     7801 tgattcaata tttgcttgtt caacctggaa acaatgacat cccaatgcat cgtgcataat
     7861 tgaggcaaaa gtactgttta aagactattt aaaaagaagc taagttttat gatgctccag
     7921 aagtgaactt ttttgtctaa cttctaggat tgtctctaaa gcttagtttc tttttcttta
     7981 tattttcata ataaaagttt ggttctttaa tttttaaaca tgacagaaaa ttttttcttc
     8041 gttatacttg taatagaata ctattttata acaaagggga tcgaatcaat gaaaaaaact
     8101 gttatttata ctagtttatt tgcagctttg tttttagctg cttgtcaatc aaataccaat
     8161 caaggcacat ctgggaatac gtcttccagt agcgaacagc gctcttcaca ggtatcggac
     8221 tcctcaacaa aagcaagcac atcttcgtct agccagacaa aaagttctag tgaagcaagt
     8281 actcagtcag atatcaaagt gtctgcagct cgtgcgatcg aattgttcca agataaatac
     8341 ccagatactg caattactag tttggaatta gatagtgatt ggggatctta cttctacaag
     8401 atagaaggtg tcgatgatca aaaagaatac gaagcaagaa ttgatgctca aaatgaaaaa
     8461 atggagacag agaatccaga aactctagat cgagatgaac aaaatggtca aaagaaaaaa
     8521 gaagatggtc tggatgttga taaattgatc acgattgagg aagcagcaaa aatcgccgtc
     8581 gacaaagtag gaagcggtac ggcaacagat tgggatttgg ataaagattt aggtactact
     8641 tattgggaag tcaaagtaaa agacggcaat caaagtacat cagtcaagat caatagccaa
     8701 acaggcgaag tactgtcaac tgaacaagat gattaagaat ttcttcggaa aacaagagtt
     8761 gatttttctt ttagtcatga tacactagac aaaataactt gtttgaagga gcgatttact
     8821 tgagtttaga aacgaccttt gccaatcata tctttgccaa tcctttgatg aatgcatctg
     8881 gtgtccactg catgactacc caggaattag atgaactggc tcattcagaa gcaggagcat
     8941 ttatcactaa gagctgtacg atcaatgaac gaaaaggaaa tcccgaacca cgctattttg
     9001 atgttccttt agggagtatc aattctatgg gacttccaaa cttaggtttt tcttattatc
     9061 tagagtatgc tcttgcgtat gaaaaagtgc aggaaaacca aaatcaaccg ttattttttt
     9121 ccattgccgg gatgagtgtc caggaaaact tggaaatgct tgagaaaata gaaaaaagtg
     9181 ggtttaatgg gatcactgaa ttaaatttat cttgtcccaa tgtgcctgga aaaccacaac
     9241 tagcttacga ttttgaggcc acttatgaaa cactgaaaga agtattcagc atcttttcta
     9301 agccgctagg aatcaaattg cctccttatt ttgattttgc tcattttgat cagatggctg
     9361 atatcttgaa tcagtttcca ttgacctatg tcaatgctat caacagtgtg ggcaatggtt
     9421 tgtatatcga tacagaacaa gaagcagtag tcatcaaacc aaaagaaggg ttcggcggca
     9481 ttggcggaga gtatatcaag ccgactgctt tggcgaatgt tcgcgctttt tatacacgtt
     9541 tgaaaccaga aatccaaatc attggtacag gtgggattcg gacaggacaa gatgcatttg
     9601 aacatctgct atgtggcgca agcatgttac agattggtac cgagcttcat aaagaagggc
     9661 cggaaatttt ttctagaatc ataaaagaat taacgcagat tatgtcagaa aaaggatata
     9721 cgtcaattga cgaatttaaa ggcaaattac gcaccatttc atagcaaaga gacgaaggtc
     9781 atgattgatc ttcgtctttt tttataaaaa acactcgtct gttcattcct gtgcaagcgt
     9841 actcattgta tgttagacga acttgtggga aaataacaga ggcaaaaagg aggataatat
     9901 gaatgatgca acagaattag caaaagagct taaagaaaaa gaatttagtt ttcaagaact
     9961 agttacggat atacagaaaa aaataaagaa gcaaaatcat gaattgaatg cgtttgtcgc
    10021 gcttgaacca ttcgagagta attcccacga gctattgatt ccccagtcag aaaaagggcc
    10081 attggctggt atcccatttc ctttaaaaat gctcggtcaa gaaaaaaagg gatggctagc
    10141 tacttcagga tctcgtttat ttgaaacaca ccgagcttct catacatcta attatgtgcg
    10201 gcaagcagaa gcgataggtc tagttccttt tggacaaacc aatgcgccag agtttggttt
    10261 caaaaatatt acagacccag tcatctatgg cccagcaaga aacccttgga accttgaatg
    10321 tacaccgggc ggatcaagtg gaggagctgc tgctgcagtt tcatcaggaa tcgttccttt
    10381 agctggtgct agtgatggcg gtggttctat tagaataccc gcttcgtttt gcggattgat
    10441 tgggctgaaa ccttcgcgag ggacgatgcc agttggtcca tatgcttgga gaggatggca
    10501 aggagctgct attgatttcg gcttgacagt ttctatgaga gacacggagg ctttatttta
    10561 tgggatgaga agtatccatt ccggtgcgcc atatcaagta tcgccagttg agtggcaaac
    10621 acatccaaga aaaaaacggt tgaagatagc tttttgtgtt gattcaccga tccactcaca
    10681 gattagtgaa gaggcagtac ttgctgtaaa aaaagctgta gatttccttg gaaaagaagg
    10741 gcatgatttg acagagattc cttacccact agatggtcgt aaactgatca cgtcttatta
    10801 tcagatgaat ggcgcagaaa cagcagcaat gatccgctcg attgaaacag gcatccagcg
    10861 agctgtcaga aaagaagaac ttgaacccat ttcttggact ttgttccaat atggtcagaa
    10921 gctctctgcc gctacttaca tcgattctct gcatgtatgg gatgaagcag caattgtcat
    10981 ggaaaaccta catcaagaat acgatctatt cctttcacca acgactgcat ttaccgcccc
    11041 taaaatcagc gaagatttgc aaagtgatgc gataagagca cagatggaac acatcagtga
    11101 ttgttctgaa gtagaaggtt tagacgttgt ttcttccatg tttgaggaga gcttgaagat
    11161 cacaccttat acacagttgg ctaatctgac tggacaacca gcaatcagct tgcctactca
    11221 tttgacaaaa gaaaacatgc ctctaggaat ccaatttatg gctgccagag gaagagaaga
    11281 tttgctattt caaatcggct atctatttga agcaaatcat cagttccatt taccaaatcc
    11341 atggaaatag cagaaacaag gagaagaaga atgaagaaaa aaagattgtt tgctctaata
    11401 actagtgcct tttttatttt aagcgcttgc ggtacagctg ccgctaatca ggaagataac
    11461 agctggagta atgtagaaaa aaagaaagaa ttagtagtag cgacttctgg cacactgttt
    11521 cccgcatctt attataatga tcaaaatgaa ttagttggtt atgatgtaga tttggcaaaa
    11581 gaggtggcta aacggttaaa tgtcaaaatc tcctttaaag aatacaacgt ggatggacaa
    11641 atcacttctg tttcaaaaag agaatcagat tttgctgcga acgattttgg tatcacaaaa
    11701 gaaagagaaa aaaaattctc cctttcagag actttcaaat attcatttga cagtatgatc
    11761 gtacgaaaaa aagatgattc aggaatccac tcacttgaag atcttaaagg gaaaaaagca
    11821 gctggcgaac ccaatactag ctatatgcgt ttagcggaaa aacacggagc taaattagtc
    11881 acatatgaca atgccacgaa tgatcagtat ttaagcgatg tagcaaatgg tcgaacagat
    11941 gttattctaa atgattatta tctgcagaaa atggccgtat ctgctttacc agatatccct
    12001 gtaaaaatac tggagaacgt ttatttcaat gaaaacgaaa gtggtttttt attgaaagaa
    12061 ggaaatgatg ctctgaaaaa gaaaatcgat ggcgtattaa aagagatgaa agaagatggg
    12121 acgacaaaga aaatatctga aaaatatttt caaactgatg tttcagtcaa accgaaagaa
    12181 acgatggctg agtcggtgag tgagtgatgt actttcctct aattgatttt tccctcatgt
    12241 ggacttcact tcctttcgta ctgcaaggct tggtatacac attagggatt ggcttcgtgt
    12301 cgtttctttt aggaaatctg gtagggttgc ttttgacagt tttaggtctt ttggattggc
    12361 tgcctctgaa tgtatttatt cgtttttatt tatcgttctt tagaggcatt cccgcactag
    12421 tcctgctgtt tctgttatat tttgggttac cttatcaatt aagtgcctta acggcttcag
    12481 tcatctgctt tacgattacc agcagtgcat ttattggtga aatctatcga ggatcactag
    12541 caggcgtttc ttcaggtcag tgggacgctg gtttagcttc tggtttttct ttctttgggg
    12601 tattgaaata tatcgttttg ccgcaagctt ttcgaatctc agtaccggca ctcagtaacg
    12661 tagctatgga tctagtgaaa ggtacttcgt tagcagcaac aatcacagtg ccagaaatat
    12721 ttcaaaaagc aaagatcata ggcggtcgga cattcgatta tttttctctc tatattttag
    12781 ttgcactgat ttattgggtc atttgtatcg ggatcgaaaa agtgcaacag tatttagaaa
    12841 aacgttctat gcatcagtca atctgaaaaa tagaaatggt gctagcgtgg ctggcaccat
    12901 ttctattttt tgtttcttgt atattctgcc tagtatttta aaaccatttc atgtaaaatg
    12961 taaatcgaaa atatgttcgg aggtagaaaa catgaagacc ccgctaacca tcgaaataga
    13021 atcctcgtta taccaatatt gcatcgagca aggcggcttg gttgtagaag aagtcacgat
    13081 gcctgatgac caaggaattg tagatacgct tagctgcttg acaaaatttg atggaagccg
    13141 agaatggcga tgctatgagc tgaaagtgac aaaagcagat tttcgctcta cagccaaact
    13201 gtcatttgtt ggacattata attattttgt tttaccaagt aaactatatg aagaagtaaa
    13261 aactgaaatc cctacaggaa taggtgtttt agtgtatcgt ccgtacacgg ttgaagagga
    13321 gatgcctgcc tctggtacat ttctgattgc gaaaaaagca gcaaagaaag agcttgctgt
    13381 accggaagaa gctttgacaa accgcttcat ggcttcttta ttcagggaag taagaaaagc
    13441 aaagcaaatg gagcgaggcg taaaatattt tccaacagat caattgtaca aagaattgaa
    13501 aagacgttca gaaaatcaag atccattttc taacgaacga tattatgaac ggtttttaga
    13561 ggaaatagct agtcaggcaa tcaatgagtt gcaagaagaa gttatcgcgc ttcagcatga
    13621 ttatgaatat ttgaggcaac aacagcagat ccgtcggttg cctacagagc cattggagtg
    13681 aaaaatatgt actatcctta tttaagagga aaacaatttg acctattagc attaaaagaa
    13741 gcactgtcgc gaggattgct ctccaataaa atccaaccag tgatcgaacc agtacgtgat
    13801 tccgctacgc ttaaaaatgt gatcgaatta tttcaaaaaa agcaccatcg aatcgctgtg
    13861 atccaaaatc cccaagtagg acaattcaaa ttattcgatc agcatgtgca cagttggaca
    13921 gtcaaagaaa atagctctgt cgtccctgca cagatcttaa cgccagaaaa tctcagtgaa
    13981 gtactggatt cacctccagc gtttcttgtt ttcgatggac agcactaccc aaaagataca
    14041 gaggtgtgga aacagttggc aggagttgat tcgaaatttt tgataccaga tactagtcgc
    14101 tttcgcatct ggctgcccga aaacaaaatt gtgatccgtg actcttttca aacgagaaag
    14161 catgttgaaa gttatgcaga taaaacagat gactttttta gtgatgacta tctttttttc
    14221 cacgcagacg gatatattgg attttctgat ttcacgatcg aaggtagccg ttatttcgat
    14281 aaagggttcc ctagccgagc gcttgctatc catctaacgt atattgatgc ttacggcaat
    14341 atacgcgtga aacattttgt ctctgacagt aacgactctg ctaaagacca agcattgaag
    14401 tttcttgaag caggagataa aatgaaaaaa tggatcatgc gtcaccatca tcaactcttg
    14461 attacttcag gaatgattga attattggct ctttaccagc aacaaaaatt ccctggactg
    14521 ggagtgttga aaaagtggtc acttatgcat catttggaac ttattagtca cctgctagat
    14581 catccaaaag attggcttaa atattattca aaagttcagg aactatatga agtgatccaa
    14641 tgattagata atgtaaatga aaaaggaata gaaaagagga aactggatgt atattcggca
    14701 aacaaggaaa gaagaagtag aagaagtggt agaaatcatt aatgccgcag cggatttcat
    14761 tgcttcgcaa ggctctcctc aatggcaaaa cgggcagaaa ccaacaaaag aaacgatttt
    14821 gaacgatatt gaaagaaaag aaagctatgt attaatagtg gaagatcaaa ttgctggaac
    14881 agctgcatta gtctcaagcg tggatcccgt ttacacaaaa atagatggta gttggaaaga
    14941 atcaactggt ccttatttat cgattcatcg tgtcgcttta gcagaaagta ctcgaggaaa
    15001 aaaactagca aaaccgttct tagaacattt gttgacgatt gcagccagca aaaatatcca
    15061 agatatccgt atcgatactt atccagctaa tgaaccgatg gaaaagacca tctattctgc
    15121 tggattcact tatcaaggga tgattgaatt tccttttgaa cacggcgaaa gaaaagcata
    15181 tcaaataatc atcaattgac tataaaatca tctgagcaaa gataaagaca ttaacactac
    15241 tgaaaaaaac aatcatatga tagccaagag gttgtaacag aagtggccag cttcaaaaaa
    15301 caagaaagaa tttccgaaaa tgcttctcaa atttttgtgg aattttagcg tattttacga
    15361 aatagctgct ttctgaaact cccttcatcg agaggtcgtg acatagcttt tgtcacgacc
    15421 tctcggctta tcctacgagt attattcgat acatttatat caataatcct ttttttattt
    15481 tatctgcagc ttcttggaac gctccatcgt ttgacacgac taaaagtgag gggatagctt
    15541 tcaaacgttt caactgtgct aaatcttcta cataaaccgc cagtctggct gaaatctgtt
    15601 cagatgaatc tccacgtttt ttcaggcgcg attgcaatgt ttctttagag atcgtaataa
    15661 agataggacg gactaccgtc ccgaaatact catataaatg ccaaaaacca tttgctgtca
    15721 aaacggcgta acaatttcct actgtcagtt tctttttaat ttctgcttta ctggaaccat
    15781 atttatgttg agcatattgg tcccactctg caaactcatt tcccttgatc atctgattga
    15841 atgtattgtc agaaacaaaa taataatcct cttgatcttt ttccccaggt cttggtgatc
    15901 gattcgtgta agtaatgatc ttttgttccg gctgaaaaat agcttctgcg agtgaagttt
    15961 ttccagaacc agaaggaccg ataaatacat agcaataatt cattgtcatt tctcctttga
    16021 tgattgaaca agtgaaaaag ctttggaaaa tatttccaaa gcttttaaaa gtaactttgc
    16081 ttttttgtat attataccag caagccagtc acaatagcac ttaaaaagct gactaaagtc
    16141 gctgttagca aaattttcaa tccgtgtttt gcgacaagat ttcctttttc ttcatttaat
    16201 cccttcatcg caccagaaat gatcccgata gaagagaagt tggcaaatga aacgaggaag
    16261 acagatataa ttgctgttgt acgctcagta aaatggaaat taccatttgc taattctgtc
    16321 atagcaacaa attcatttgt aaccagttta gtcgccatga tactaccggc atccactgct
    16381 tctttccatg ggattccgct gatgaaggca agaggggcaa agatgtaacc taggatctct
    16441 tggaaagtga tgccgaatat cccgtggaag atagcattga tcatggcaat caaagcaaca
    16501 aaaccgataa gcatagcagc aaccgtgatc gccacatgga aaccatcaag aatgtattct
    16561 cccaacattt ggaaaaatgt ctgttctttg ttttcttcaa tgatcagctc atcttctttt
    16621 tcttctaata catagggatt tacaattgaa gccagaatat aaccgccaaa taagttcaaa
    16681 acaagggcag tgatcacata tttagaatcg atcagtgcca tataagatcc gacgatcgac
    16741 atagaaactg tagacatagc cgatacactc atggtgaaca aacgtttctc agataggaag
    16801 ggtaattcct tttttatgga aataaaaact tccgattgtc ctaaaatagc agaagctact
    16861 ccattataag attccaattt gcccatcccg ttgattttac ttaatcctag accaacagct
    16921 ttcataaata atggaagaat cttgatatag cgtagaatac cgataatagc agaaataaat
    16981 acaattggca tcaatacgga aagaaagaaa ggtgcacttc ctttatttgc tacaccggca
    17041 aaaacaaagt caacaccttg cccagcaaaa gcaagaagat gatcaaacat ttttgccaat
    17101 acagaaacaa ccgctgtacc aaaagttgta cgtaataata cgaaaccaag aacaagctgt
    17161 aaaacgaaca tcacaattag tggtttcaat ttgattgctt ttttgttact gctgatcaaa
    17221 aaagcaagcc ctaatgtgac aaataaacca ataataccta ataaataact caaataatca
    17281 ctcctattcc aaattccgtt ttattttacc tgttttgcaa ataaaatact aatctttagt
    17341 gaaattttca ttactttttt agagtgaaac gcattcattt tccattagaa aataaagcga
    17401 acaaaataaa aggtttaaaa agtgtaacgt cttagtaaag tctataaaaa aacgaaacta
    17461 tcgattaagc taatagtttc gttttcaaca tttataagta aactttttct attggtaata
    17521 tagattttcg gaagggtaga caggtgcgca ggtaatatcg attcctagtt ttttcaatgt
    17581 ttgttcatca tttttattga ccatcacagt agagtgggct tgtgctccct caagttcagc
    17641 tagtttatca taagcaactt gagcgattgg attcgttact gcactgattg ataaagcgat
    17701 caagacttca ttgatgctta gtgcatccaa agaggaatga agtgtctttg atttcaatcc
    17761 ttgtattgtt tcaagaacta atggtgacaa taaaggaatg tcatctgcga tatgggctaa
    17821 atgttttact gcatttaaga taactgccgc tgacgcatcc atcaatgaac ttgtacgccc
    17881 ggtaatgatg acttcatctg gtaattcgat tgcaatcaca gcagaaggat cttttgtagg
    17941 actttgcgct tgaatcttat ctgcatagtc atgggcaggg actaccacgt ttcggtcttc
    18001 aggacgtaaa ccgacttctt ccataatcag tttgattcga ttaactgctt cttcgtcgat
    18061 caatcctttt ttgaagtcgc attctgtttg gaaataacga cggatgattt cttgtttaga
    18121 ggcttcttgg acaacggtat catccacgat cccaaatcct acacgattca cacccatgtc
    18181 agtaggagat tgataaacag attctttacc agtaattttt tcaataatac gtttgattac
    18241 aggaaatgtc tcaacatccc gattatagtt cacagctact ttattgtagg catcaaagtg
    18301 gaaggaatcg atcatattga catcttttag atcgactgtg gctgcttcat aagcaatgtt
    18361 cagcggatgt ttcaacggaa cattccatac tgggaaagtt tcgaacttgg agtaaccagc
    18421 tgcatgtcct ttttggcttt catgatacag ttggttcaag gaagtagcga gctttccact
    18481 tcctgggcca ggagctgtca ctacaacgat tggtttagtt gtttcaatat actcattttt
    18541 tccaaaacca tcttctgaga cgattttttc tacgttgact ggatagtctt cgatttctga
    18601 gtgtttatag accttgatgt ttcgtctttc tagtttattg ataaagactt tcgtggcagg
    18661 ctgtccacta tagcgagtga taacgacact gttgattaat aagccataac cacgtaattc
    18721 atcgattaac cgcaagatat ccatatcata agtaatccca taatcaccac ggatcttatt
    18781 acgttcaata tctccagcat agacgcaaat cagtatttca gcttgatctt tcaatttttg
    18841 cagcaatttg attttcgcat cttcttcaaa tcctggaagt acacgtttcg catgcttgtc
    18901 gtcaactagc ttcccaccaa attctaaata taatttgtca tagtggttca ctcgttccaa
    18961 aatatacgct gactgctctt caatatattt ttctgaatca aaaccaattt ttttcactag
    19021 gttccctcct taagtactaa agcatttttt catttttttg aggattcgtc aactggaaga
    19081 gcgaaattcc tcaaaaaaac aaagaaaatg gtttaaacca acttgtctag atagttattc
    19141 attggtatga tagtttttga taaggaaggt gctacatgaa aaaaagacag aaaaagaaaa
    19201 acgcatataa acactatatc cgttcgattt ttaccggtta tgaaagaatg cttgaagatc
    19261 ccgaattgga acaattgacg tttacttatc taaacgaaga aacgcagcta actcgtgatg
    19321 agcatcagcg cattcatttc acaacaagag atctgcctag taaataaaaa ataagataaa
    19381 ggtcatcgga ttcgattctg tcctatcaga tggaaagact agttgatcga aaaaaaggct
    19441 gtaccaaatt ttggtgcagc ctgttttatg gagcattgcc cgctcttttt tataacaatt
    19501 ttttacagaa aatggtacta ttgaagtatt aataagtgaa aaacagatga gtaagaggag
    19561 gcaagtagta tgaaagagcc agatacaaat gcaaattatg agccagacga actagaggag
    19621 acgaatgcgg atcaggatac aggacaagaa tcgtcttctt tttacgatat caaagaaaaa
    19681 gcaagcatga atctttccca attattagaa gacttacagg cttttgaaaa agctgtccgc
    19741 gaagaagata tccctgagat ctaccgttta tacaatggcc gattgaatga agaattaaaa
    19801 agaagctcaa atgaaaatca cgagatcgat caattgctga tgcgtaagat ccatgatcgt
    19861 tttttacagg aatttccttt tatggaacaa gtagaaaaca tctcgccaac gatgagctat
    19921 tacaaaatcg gcacttatta ccatgatcgg cccacggtcg gtattgatgc aagtctgcca
    19981 gaaatttttg ttttgcctaa gatcgatgaa gagtgggaga aatattccca accggagtca
    20041 acggactatg acaaaaaaat caatgagcta gacgcaaagg tgatcactgc tcagacagaa
    20101 attgaacgtt tagatgaaca aataaaagaa atcaatcgac aaatgactga tttgaacgac
    20161 aataaaggat tcttgaatcg taaaaaaatc gaggaagaaa ttcaagaatt agagaagaag
    20221 aagcaagtac tatctaatga aaaattaggc tggcttcctt atattgagac gcctgaaaca
    20281 atccagcagc aaaaagaagc attgaagcaa gaagcaagag cagaccagct tcgtgcagcc
    20341 atcgtagaaa aagaacaaag gcaaatcaga cgctatttcg gtagcaaaga gggattcggt
    20401 caagcaatcc atgaattctt gatgaattat cttggcaatg agaatcctaa taatcaatct
    20461 aatgaaggag gttccgagta tgaatgaaca cgatgaagca gaatggacaa aaaatgaaga
    20521 acaaacagaa gatcatgaag aaacgaacac ttcattggaa gaacaaacaa gtaatcctct
    20581 ggatgacaca gaagaaatcc ctatgctaga ggaaacaaaa atacacgaaa aagaaaaagt
    20641 aactccgacg aatacgaata ctgcagaaaa cacccatgct tacgaaataa ctgaaaatcc
    20701 cgggtattta gaaggaaaag agcaagatca tcaagatatc gtcagtttga ttgaaacagc
    20761 ggaagcagaa ttagcggatt tacgtatgag aaaagatttg atcgaatcag attttccaca
    20821 attattagaa tcatatgatc gttttatatt gaatgaagaa ccaatctcca cgatcggaaa
    20881 gacaagcctg atcgaatact tcacgtatga attactgaaa ccgttgaatg atattggtct
    20941 ggaacggtca aattcagctt atgacacatg gaaaacaaga catttttcga tcaattatac
    21001 gttgaatgga caaaacgaat tagtcttttc ctttgtatta ccgaccatcg atcaacgata
    21061 ccaagtggaa tcgatgagtc tcctaaacgt atcaccagag acaatggaaa tcaatgtcca
    21121 agatgaccgg gtgctttctt tgatccgaca ctggtcagtg gatcgagtat tctctgcagg
    21181 gcagatcacg atattcaatc acaaattgaa tcaattatta gcccatgcaa gaacacttgg
    21241 ttttaccgtt aatcagacat tactagacaa tacgaagcca cttcgtttga atcttcagag
    21301 cgaatttgaa ttgacgaatg aagtattgga tgatattttt atcgtggcaa tgaaaaatcc
    21361 atcctacgat atggaaaaga tcaacgaaac tacttatcaa gtacgattag ataaaggaca
    21421 gtctttaacg ataacaacaa acgaaaaaga tcaaacagtc ttgtcgatct catctggaga
    21481 ctacccacga tctgtcattg atttctttat caattacgaa ttcttggtcc cattaatggt
    21541 gagaaagtcc tgattggata agttggatta ccagatgtca ttcagataat tctgttccaa
    21601 aggacttgcg gcagttatcc agaataattg tcacaagtct tttctgtatg tactaaatgg
    21661 acgtgaataa cttgctgatg aaggagggat ttcattagga atgtccacaa ataaaatagc
    21721 acagcttagc ttattgtctg ctgcttgtgt agcaggtagg atcgcttttc aatttattcc
    21781 gaatttccag ccaatgactg ctattttttt atttatagta ctttacttaa gcttaaaaga
    21841 tgcgttaatt gtcatgagtc tttctattgc gatcagcagt ttttattttg gtgttggacc
    21901 atgggtcatc ggccaatgga tcagctatac tgttgttctg agtttgtttt caatcgtctg
    21961 cttgagaaaa acagtgcaaa ggaatctatg gttaaaggga gtctgttttt ttctggcggg
    22021 catggtttac ggaataggta tgaccgtttt tgatacgctg ttatatcgtt tgcctcaacc
    22081 ttggatctat taccttcaag gggtgtcgtt tgatttgatg cacagtatag gcaatgttgt
    22141 gttctttctt gtttttctgc cagtcgtcaa acgtttttac aatcattcag gaggaaaaaa
    22201 tgaaaaaaat aatttatagt ttgcttgcaa tgagtgtcag tatactcatt ttgacaagtt
    22261 gcagtacaac ctatacacaa tcggcttcca accaaaagga ggaagtaaaa acatcgcaaa
    22321 aagcaacaat cacgttacaa gaaaatggtc aaaacttttc ggaaaaagaa gtgttcttca
    22381 aaaaaggcga tgatttactg agtattttga aacgaaattt tgaagtcaaa gaagataacg
    22441 gcttcatcac ttcgcttgaa gggcactcac aggatgagaa aaatcaactc tattggatgt
    22501 ttactgtgga cgggaaaagt gctactactg gtgccaaaga gatcaaattg aatgatggac
    22561 aatcggtcat tttcaatttg tctaaactat aagtttcata aaaaactgtt aaagggcgaa
    22621 tattgccata attcagtgag ttcagttgat gaaagaagga aaataacatg gaaaatttta
    22681 aaaaaagcgg aatgttttcc gtaattgctg ctctaggggc taatattcta gttgctatca
    22741 gcaaatttgt cggctatgca atcagtggca gtgccgccat gctaaacgaa agcatccaca
    22801 gtatcgttga ttgcagcaat cagattttgc tgttattcgg tgataaacaa gccaataaag
    22861 gacagagcga gttgcatcaa tttggtgaag gacgagcaaa atactttttc agtacaatcg
    22921 tcgcaatgat gcttttcttc ggcggtgggg cacttggggt gatggaagct tttgaaaagt
    22981 tgacgcatcc atcccatgaa gtgggtaatc cgatcattgt tatagcaata ctgttatttg
    23041 gtttggtaat cgaaggaagt tcgttacgcg tggcaatgaa agaaatcaaa gaattgaata
    23101 ctgaaaaact ttccacgatg cgctttttgc gcgaaagtcg acacagtgaa atactgatta
    23161 tttttacgga agacttgtgt gctgtaatcg gtttggtctt ggctttagca ggaacaatat
    23221 tgacttatgc aaccgggatt gctgcatttg atgcgatcag cggattgctg atcggtttct
    23281 tgctgatggg agccgccatt ttcctagcaa aagaatttta tagtctgatc atcggtgaaa
    23341 gcgtgacagc aagcgattta tcaaaaatca agttagcttt tgaacgtcct gagatcagtc
    23401 ggctgatcga tataaaaaca gttcatttaa gtccaacgga gattttagtt gcagcaaaag
    23461 cagatatcgt tggtaacaaa gagaatgaag cttactcggt catcaatgat attgaaaaaa
    23521 atatgcgtca gtctcttcct gacaagaaga tgtatatata cattgaaaca gataaatatg
    23581 acccaaatta ttctcgtaaa taaaacgttt attcatgaat tgaagcaaaa atgagaaaaa
    23641 tataatcttg tttttaaata aaacggctac aatttagcga atttatgtta caatgagtcc
    23701 gctgtttatt tgttgtaatt aaaggagata ttttaatgaa caaaaagaaa agtgcgccat
    23761 ggcttcaagc tttgcatgca gtgatgccac tttgtataag ctatatacca gtaggattag
    23821 cttgcggagt attgttgcaa aaagttggat ttgatcctct cttatcagga ctgttatcga
    23881 ttctggtctt ttccggtggc gcgcagtttt tagttgcatc tatgcttacg acgcaagcat
    23941 catttgcaac gacattattg atggttttct ttttagaatt acgctataca ttattaggag
    24001 caagtcttgc cggttttatg aaaaaagaaa agcggacgtt tttggctgtc ttttctcaat
    24061 cactgaatga tgaaaactat gcggtgaact acttaaagtt ttcaactgac ccttcctgga
    24121 ataaaagaaa ggctttatat gtcaattggt tctcaatgac tgcatgggcc ggtagcaata
    24181 tgatcggtaa ttttttcggc tcggtgattc gagtagatgc tgaccttgtc cattttgctt
    24241 tgacggccat gttcattttc atgtttatca tgcagatgaa aagcgcacta ctgatcttta
    24301 caggtttatt ctcaggcgta ttaggggtcg tattcatggt attattccaa aatactttcg
    24361 gattgattgc agcaacagtc attgcttcat ttgccggatt tatgctggag aaggcattca
    24421 aaaaagaaaa agctaagaaa ctaagagacc gcggtaaaga tgtcaatgag ccgctcttca
    24481 acttcccgga acaggaggcc aatcaccatg actaaactat atttgctact tttagttgct
    24541 tgtctgttca tcgtggcata tatcccgcgc ttgttcccga tgctttattt tactcatcga
    24601 aaagtgccat cctggttcag tgactggatg aaatatgtac cggtggcatt atttgcggcg
    24661 ttagctttta aagatgtttt cattactcat gaacatctgg atattgcttg gaatatcaaa
    24721 atagctgcaa tgattctagt agcaggcgtg gcttacaaaa cacgttcgat ggcgttgtca
    24781 gttttgacag gacttgcgtc tgtctttttg ctgtctatgc tttaaactgt tttattttat
    24841 taaaaacaga gattgcgaca taagttgttc aaactgatct atccgaactg catttgcaac
    24901 agtcaattct ggatgcaagt ttattcgtag ggataaaaaa gattagtgtt ggcttatgat
    24961 gcaagctctt ttttatttta ggaattagag agggatttgt ccttctatga ttttttgcta
    25021 taatagggaa gatgtttatt ttatgaaaga atagttgagg gaatttaaat gaataaaaaa
    25081 ttgatgcaag gaacattttg gctaaccttt gctaatcttc tatgtaaagt tttaggggta
    25141 gtctatttga ttccctggct aagtatgatg gggaacaatc aagatggtat gcttgctacg
    25201 acattgtata atgttggcta tcttccttat ggattgttcc tgatgcttgg tacagtcgga
    25261 tttccaaatg cgattgcaaa aaaagtagct gtagccacaa aagaaggaga taacgctgct
    25321 tgtcggctga tttttagaag tacgatcaat atcatgtttg tcatcggtat cgcctctgct
    25381 gcactgatgt atttatttgc tcctttacta gccggaatca gtccgattgc taacgttaat
    25441 aatgggatac tagctatcag aagtttatgc ccatctttga ttgctatccc aattcttagt
    25501 gcaatgagag gatatttcca aggaaaaaat gatctgcggc cttatggaac ttctttgatt
    25561 atcgaacagg ctgtgcgagt gatcgttatc ttggctggta cttattactt gcgcgtattg
    25621 actgacggaa ccatattaga agctgtattg atcagtacag tcgcttcttt cttcggtggg
    25681 ttagctgcga ttatccatat gtatatcgtc ggacgcagaa aagacttctt cgaacttaaa
    25741 gattttttga tttccagccg ttatttcgaa agagaaaacc gatctgcctc tgtctctatc
    25801 attcgggaaa cactaccgtt tatttttgtc ggctcagtca ttactatcgt tcaaatgatc
    25861 gaccaagtaa cgatgaaacc actgcttcac tttttccgtc cagagatcgc agatcagcaa
    25921 ctggaattct tattcagccg tgcttctgtc aatccaaata aattgacatt gatcctgatc
    25981 tccatggtag gaacagtagc tataagcagc ttgccaatat taagcacgat gaagaaaaaa
    26041 gatcggatac aaatcgaaaa aacagtagga gatagttttt cgattgcgtt attgattctt
    26101 cttccttcat tgactggaat gtctttatta gctggaccac tatacacatt gttttttggc
    26161 tatgatcctg aaagtgtcgg atatttccaa atggctttgc tagcttcttt gtttttctct
    26221 ttattcacga tcctatctac gatgcttcaa tctttgaatc accatcgttt tgcgatccga
    26281 ttaactgtag aagcaattat cctgaaagtc atcttccaag taatcggatt aggattagtt
    26341 ggcggatacg ggatgagctt atcaaatggc ttagcattcg gtattgtctt tgttcgcggc
    26401 tatcacttta tgtgcaaaga ataccgaata gcacctttag caaagatcag caattttttc
    26461 ttaaaaactt tccgctccac cctgattatg cttgcagtat gttttgccgt tttctttttg
    26521 ctgaatcagc gattgtcaat ggaatcaaaa agctatgcaa tgatttattg tgtggctgta
    26581 ggaagtattg gcggcttagt atttttattt tctcaattcg gaaaaaatgg gtttcgaatg
    26641 ttgaaaaatg ttaaacatca tagcagaagc aaagaatagg ttaactaatc gttttttgct
    26701 gtcacttgaa aaacggcaac tcgtatgatt acgcttgtcg tttttctatt ttccaatttt
    26761 agtttcatat ctaatcttac ttatgatata gttataaggt ctggaaaaat tctttgaaac
    26821 gaggaaatta aacacatgaa acttgcagta gttacagata gttcggcata tctgccggaa
    26881 cgtatcaaga accatcccga cttattcgtt attccaatcc ctttgattat ggatgggaaa
    26941 atttataacg aaggaatcga tatcgaagcg gatgaatatt atggactttt aaaaaacagt
    27001 aaagattttc ctactacttc acaacctgtc gtaggggaag ttttgggttt atatgaggaa
    27061 ttgaaagaga aagggtatga cacgatttta agtatccatc tttcttcagg gatttctggc
    27121 tttgtcaaca cattatttgc gatgaaagat gacatcagcg gagtaacggt cattccttat
    27181 gactctaaga tcactagtat gccgatggga cacatggtag aagctgcctt agacttaaat
    27241 gatgaaggtc agtcccttga tgaaatactt gcacatatcg atcgaatccg agataatacc
    27301 tacgcttatc tgattgtaga tgatttgaac aatttagtac gaggaggacg tttgacaaat
    27361 ggtgcagcgc tgattggcag cttattaaaa atcaaaccga tcctgacatt ttctgaaggg
    27421 aaaattgttt tattcgaaaa aatccgttca agtaaaaaag catttgctcg agctgaagaa
    27481 attattggta gaagaaataa cgaaatacaa ccgccagtca aactttacgt gatacatgca
    27541 aataatttag aagtagcgat tgcggagaaa gagaaattgc agaaaaaata tccaaatgca
    27601 gaaatcgaaa ttggtcattt cggaccagtc attggtacac atttaggtga aaaagccatc
    27661 ggcattgcca tttcagcgca ataattcctt ttattcaaaa aacataccaa aaatattcca
    27721 acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga gatggtgttt
    27781 ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct ctataatgtc
    27841 gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa ataaaaggat
    27901 gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta atcgttgtag
    27961 aagactatga taaagttgat ggaagaaatg tattaaatac ggatgcaaaa gggctttcac
    28021 tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag gtatggcgac
    28081 atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt attttagatt
    28141 tagcgatctt agctgcacaa ggaagtttat acttccaaga tgcttatcgt tatgaaaaat
    28201 tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac cgaatgacgg
    28261 tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat tacgatacac
    28321 tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt cttttagatg
    28381 agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg agaatttctc
    28441 actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga agaaaccgaa
    28501 aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag ggaattggca
    28561 agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt cggggaagac
    28621 acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag tgacctgttt
    28681 tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag attctaataa
    28741 ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg aagctgtact
    28801 gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa aattagcaga
    28861 gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct attacccttc
    28921 tgaaatcgtt cgagcgattg aaaaaggata tttattagag attcaagagc cgactgtcat
    28981 ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg ggatgttgaa
    29041 tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca ctacaaatcg
    29101 aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc aacatgcgga
    29161 aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga aaactatgat
    29221 ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg atgaaactgc
    29281 aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt gggcaaatac
    29341 ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt ataaattatc
    29401 cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg gattgctact
    29461 ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg agcatacaaa
    29521 aggaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa atgctgaaga
    29581 aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg agggagaaga
    29641 gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa aagaacgcag
    29701 tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt ctcaagaaat
    29761 gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga ataaagaagc
    29821 gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc accgaccagc
    29881 tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc caatagtaga
    29941 atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga cgtaccaaaa
    30001 aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg atttgagaaa
    30061 ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg tacggatcga
    30121 tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag cagcgattgc
    30181 cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag atacagcaga
    30241 ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag acggatttca
    30301 atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg atggtgctgc
    30361 acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac tgttgcttaa
    30421 tattagtgac ggtcagccaa aagcgttgcc ggattataca ggcaaaaaag caaaagaaga
    30481 tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct ccgcagctat
    30541 tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg tagatattac
    30601 tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc tatgataaaa
    30661 taaagctcaa cgaacgcact tagcagattc gttgagcttt tcgtttctag ttaccggtaa
    30721 caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa ggagtgaaac
    30781 gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat ctatcctgct
    30841 agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg tgaaaaactt
    30901 aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta tcaatctcca
    30961 tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag attcggaacg
    31021 atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa agtgattatg
    31081 gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc gaaaaaatct
    31141 aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg caaagaaccg
    31201 aataattgga catcgatttt cggcggttct gcttgggagt attctcatga aactggacag
    31261 tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag cgagaaattg
    31321 aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga tggatttcgt
    31381 ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac agagaaccct
    31441 ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt gaaacatgtt
    31501 tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc agaagaagga
    31561 ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga tcatatccat
    31621 atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaact tgcattaagc
    31681 gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga aaaccatgat
    31741 gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc tatgtctgca
    31801 aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta tcaaggacag
    31861 gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc agtagattcg
    31921 aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc attggatatc
    31981 attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc ggaacaatat
    32041 gctggttttt ctacacatga accttggctg atcactaatc caaataccaa aacgatcaat
    32101 gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa catgatccgc
    32161 attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct tcatgaacat
    32221 ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat tatggtaaac
    32281 ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc ctggacatta
    32341 ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc accttatgaa
    32401 gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta gttggacttt
    32461 gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg ctctaggacc
    32521 accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa gggttttcaa
    32581 acgaggtctg acaggtactt ggacatgttt gatatgcata ccgattgatg tgtcaccgat
    32641 atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga actggtcaaa
    32701 agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga caatttcgaa
    32761 attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat gttcacaacc
    32821 ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac ggatgatcca
    32881 ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt cgcttgtgct
    32941 gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata tgtcattaac
    33001 tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct aaaaccttct
    33061 ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt ataggaaagg
    33121 caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc actccagccg
    33181 gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact aagcttccta
    33241 ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca gcaacatatc
    33301 cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct gaaatcaaat
    33361 cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca taaagaaaag
    33421 cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc ggtacaggga
    33481 tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag atcgttaatt
    33541 gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac acttccggta
    33601 tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt actttctgct
    33661 actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga agaaaaggca
    33721 caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac ctcataatga
    33781 tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc gcctttgatc
    33841 acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat ttctgttaac
    33901 gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg cgtaatcatc
    33961 gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt gatcacagct
    34021 tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg ttttgttttt
    34081 atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat tttgatacca
    34141 tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc caaagatata
    34201 gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa ggcgcttagt
    34261 ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc accagcaaat
    34321 ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat gaataagagt
    34381 ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct catccaattt
    34441 aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa tagttaggac
    34501 ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt agctgttcgc
    34561 gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc atggacttgc
    34621 tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat cggttgtgga
    34681 gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga atctaataaa
    34741 aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg ccgatctttc
    34801 aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca ccaatcgcca
    34861 taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg tcctttagac
    34921 agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga atctgtaatc
    34981 cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact tgttttcaaa
    35041 ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc atatacttga
    35101 agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt ctggttgttt
    35161 atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg atgctcctta
    35221 gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat tggccggtga
    35281 gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat cataggaaat
    35341 cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg agagatgtga
    35401 aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca tttcgtccgc
    35461 catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt accagaagta
    35521 tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga caaagcaggt
    35581 tgttccatta aataaagaat agg
//
LOCUS       DACECZ010000013        34773 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.013, whole genome shotgun sequence.
ACCESSION   DACECZ010000013 DACECZ010000000
VERSION     DACECZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.013"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1537)
                     /locus_tag="IWO35_000684"
     CDS             complement(<1..1537)
                     /locus_tag="IWO35_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ0315761.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFE"
     gene            complement(1541..1951)
                     /locus_tag="IWO35_000685"
     CDS             complement(1541..1951)
                     /locus_tag="IWO35_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ0315762.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(1948..2871)
                     /locus_tag="IWO35_000686"
     CDS             complement(1948..2871)
                     /locus_tag="IWO35_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ0315763.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2868..3746)
                     /locus_tag="IWO35_000687"
     CDS             complement(2868..3746)
                     /locus_tag="IWO35_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0315764.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3733..5070)
                     /locus_tag="IWO35_000688"
     CDS             complement(3733..5070)
                     /locus_tag="IWO35_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0315765.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5073..6176)
                     /locus_tag="IWO35_000689"
     CDS             complement(5073..6176)
                     /locus_tag="IWO35_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0315766.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6250..7758)
                     /locus_tag="IWO35_000690"
     CDS             complement(6250..7758)
                     /locus_tag="IWO35_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0315767.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7724..8416)
                     /locus_tag="IWO35_000691"
     CDS             complement(7724..8416)
                     /locus_tag="IWO35_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0315768.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8438..9412)
                     /locus_tag="IWO35_000692"
     CDS             complement(8438..9412)
                     /locus_tag="IWO35_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0315769.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9712..10584
                     /locus_tag="IWO35_000693"
     CDS             9712..10584
                     /locus_tag="IWO35_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0315770.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11042..13240)
                     /locus_tag="IWO35_000694"
     CDS             complement(11042..13240)
                     /locus_tag="IWO35_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0315771.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13472..14464
                     /locus_tag="IWO35_000695"
     CDS             13472..14464
                     /locus_tag="IWO35_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0315772.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14524..15945
                     /locus_tag="IWO35_000696"
     CDS             14524..15945
                     /locus_tag="IWO35_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ0315773.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            15938..17437
                     /locus_tag="IWO35_000697"
     CDS             15938..17437
                     /locus_tag="IWO35_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ0315774.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17476..18870
                     /gene="uxaC"
                     /locus_tag="IWO35_000698"
     CDS             17476..18870
                     /gene="uxaC"
                     /locus_tag="IWO35_000698"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ0315775.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19029..19712
                     /locus_tag="IWO35_000699"
     CDS             19029..19712
                     /locus_tag="IWO35_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ0315776.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19714..20550
                     /locus_tag="IWO35_000700"
     CDS             19714..20550
                     /locus_tag="IWO35_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0315777.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20561..22315
                     /locus_tag="IWO35_000701"
     CDS             20561..22315
                     /locus_tag="IWO35_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0315778.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22591..22932)
                     /locus_tag="IWO35_000702"
     CDS             complement(22591..22932)
                     /locus_tag="IWO35_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0315779.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23000..25171)
                     /locus_tag="IWO35_000703"
     CDS             complement(23000..25171)
                     /locus_tag="IWO35_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0315780.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25382..26239
                     /locus_tag="IWO35_000704"
     CDS             25382..26239
                     /locus_tag="IWO35_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0315781.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(26310..27167)
                     /locus_tag="IWO35_000705"
     CDS             complement(26310..27167)
                     /locus_tag="IWO35_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0315782.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(27152..29854)
                     /locus_tag="IWO35_000706"
     CDS             complement(27152..29854)
                     /locus_tag="IWO35_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0315783.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(29867..31156)
                     /locus_tag="IWO35_000707"
     CDS             complement(29867..31156)
                     /locus_tag="IWO35_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ0315784.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            31306..32352
                     /locus_tag="IWO35_000708"
     CDS             31306..32352
                     /locus_tag="IWO35_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0315785.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            32354..34507
                     /locus_tag="IWO35_000709"
     CDS             32354..34507
                     /locus_tag="IWO35_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0315786.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        10192 a         7168 c         6277 g        11136 t
ORIGIN      
        1 actcaaaatc agcataactt cctctttttt tatttagatc atgcactgct atcccgataa
       61 aatttccagt aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc
      121 attcttcagt tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt
      181 tcaaaaaagt cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt
      241 tttctgattc tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat
      301 aaatataatt ggattcattt aagtacagta ccagtccagc catctgatta aaattattag
      361 gcatgtattc aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca
      421 aatgatgttc gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc
      481 tacattttgt atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct
      541 ttagctctcc ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat
      601 ttgtctcaat cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat
      661 cgtcggacgt ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt
      721 ttaatggtct ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc
      781 tcccatgtcc tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa
      841 aaccaggatc aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat
      901 gtcctcttcc tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca
      961 aatgtggtgc ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat
     1021 caagcttctt cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg
     1081 tgtccagacg ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat
     1141 ggaacaaaga aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg
     1201 tgatctcaga tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt
     1261 tgacatctgt gtaaacaaga taaaacaatc catcagcata actgatctgt ggcgcccata
     1321 tacttccatt tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat
     1381 gtgaccagtt gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag
     1441 aggagacaat aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc
     1501 ctcttaaaat aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc
     1561 ttactcgata taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa
     1621 taaactgagt gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg
     1681 ttaagtattt tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa
     1741 acaacacaac ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag
     1801 acaaagttga atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca
     1861 tattacttaa agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca
     1921 ttcctagcaa tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat
     1981 ccattcaggt cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt
     2041 ctgttcattt ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat
     2101 cttacacgct tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt
     2161 tctagtatca ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt
     2221 tttcggcgta gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg
     2281 acactgatga aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca
     2341 gcaatattca taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg
     2401 agtaccatct ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga
     2461 tagtcgacag ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc
     2521 taccttttct cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga
     2581 tccttctaag aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc
     2641 ctgttttgca aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt
     2701 ggataatctg ctttctatat attcatcata tcttccttct tttactatca agacctttgg
     2761 atggctatcc ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt
     2821 cggggtatct atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc
     2881 cttatgaatt gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa
     2941 gaaaaagaag aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt
     3001 ctcagcttca gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga
     3061 cgagggcgcg gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt
     3121 ggcacatagt aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg
     3181 tccacctgtg ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac
     3241 cagcttaata agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct
     3301 tcccttgtct ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac
     3361 gtttttcctg ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct
     3421 tcaaatcccc aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca
     3481 aaagatggag aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa
     3541 tctccgccgt gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa
     3601 gcatagaggg ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata
     3661 aaaccgtttt gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga
     3721 gcacctaaaa aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat
     3781 attttctgat tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag
     3841 aaatgctggt ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa
     3901 gccaatataa aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc
     3961 gggaactgca tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa
     4021 ttgttccgct aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt
     4081 cgaaaaatgg atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc
     4141 ccatacatac ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg
     4201 acacatcaca ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg
     4261 acctcgtcta gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat
     4321 ggtaatatta cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg
     4381 gaccatcgtg caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc
     4441 agtccctgcc ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg
     4501 cacatttttg catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa
     4561 gatcgtcaag ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg
     4621 tgactggatc aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa
     4681 tttaggacga ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt
     4741 tccatttcca tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata
     4801 gatacaggaa gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata
     4861 atcttttgga tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt
     4921 gctttttaaa aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc
     4981 tccgtcactt gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat
     5041 tgatgcgcca aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct
     5101 tgcaagaatg caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa
     5161 gcatatgaac catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc
     5221 aagttcagcc agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat
     5281 ttcttcactt gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc
     5341 attgcgtaaa caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt
     5401 tctgttgtta cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc
     5461 agtgtctttt ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca
     5521 taggtatctg ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat
     5581 agacctgttt ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc
     5641 aaatttgttt ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct
     5701 tggtaatctt tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata
     5761 tataagccat ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg
     5821 gatgtgcgtg gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt
     5881 tcttcgcctg tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata
     5941 ttgtattctt caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata
     6001 ttgtctttta tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac
     6061 cctttaagta cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat
     6121 aagcgaggtg ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt
     6181 tgctctcctt taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa
     6241 tcgtctatat tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc
     6301 gcctccttgt gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc
     6361 catgatgtat tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt
     6421 gttcgtcggt gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt
     6481 gatcaattga ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc
     6541 actaggacga gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa
     6601 ttcgatagaa ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa
     6661 tttatacaca tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc
     6721 ttcgtctttt acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga
     6781 ccaaacatga taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat
     6841 atcatcttga ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta
     6901 gattccagtt cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa
     6961 gtcttgtgcc aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata
     7021 ctcttctgta tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg
     7081 tgctccaaaa taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg
     7141 atcgccaaaa ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg
     7201 tttagctact tcataaagct catccatggt agttggaaca tctagtccta gattatccaa
     7261 ccagtctttt cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta
     7321 caaggcaccg tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt
     7381 atagtctttt aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac
     7441 agaagagttt ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc
     7501 caatgcagtt gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc
     7561 cgtatcggtt ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg
     7621 caaaatattt aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact
     7681 tgaactgctg ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac
     7741 gagcgttgct tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt
     7801 ctgttttttc tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc
     7861 acgtgactgc ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt
     7921 ggatgatcat cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat
     7981 gtgagaatcc gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat
     8041 gagccatgat tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca
     8101 aagcgatcct aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga
     8161 tggcgccata tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga
     8221 agtacgtttt cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa
     8281 ccatcgcata cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc
     8341 cccacagaaa attcaaataa accaactgta aaatacgatt tagccttccg tttattttat
     8401 ctaatgactg cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta
     8461 gctttttggc aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca
     8521 atcccattgc tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg
     8581 tgtcgaaaat ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga
     8641 accctaagtc taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa
     8701 ttgccggcaa tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag
     8761 cttcatacaa ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc
     8821 ccaactcttt ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta
     8881 gaaaactgat tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa
     8941 cattcaaaaa tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt
     9001 aaatgatcgt ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag
     9061 acaaaatgat tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat
     9121 ttcttaataa tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga
     9181 cccaaggact tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc
     9241 cccccatagg aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat
     9301 ataaaaattt attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt
     9361 cttttttcga tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct
     9421 ccttagtttt gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg
     9481 tatataatca tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac
     9541 aaaacgataa agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt
     9601 ctatctgaca cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg
     9661 aacatataga aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag
     9721 aaagtctcta taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta
     9781 gctttgatct gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa
     9841 gaagtcgtcg cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc
     9901 tacattcttt ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt
     9961 gtcggaacga tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag
    10021 tatttgtttg gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg
    10081 gggatgattc caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc
    10141 atgatcctgc ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa
    10201 gccttgccgg atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta
    10261 tttttcaaaa ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat
    10321 gctgttactt actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa
    10381 tggccgatac agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa
    10441 agtagtgccg ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt
    10501 gcaacagtgc ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca
    10561 ctcgtaggtt ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa
    10621 gtggtcagct tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa
    10681 atttcggctt atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa
    10741 gagacgttca gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac
    10801 aggagcaata agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta
    10861 ttgtcgatag cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt
    10921 ttgtcacagt tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac
    10981 tagggttatt ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga
    11041 ttcacaaata tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac
    11101 gaataaaatt ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt
    11161 tagtctcttt caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa
    11221 atttttctcc tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc
    11281 tatcagcgat cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa
    11341 tattctccga caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg
    11401 gcagaactaa ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg
    11461 cttttacata aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc
    11521 ctaattgaac gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt
    11581 tattttgtgc aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc
    11641 cagaacgaat ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt
    11701 tttcatgagc agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta
    11761 cacgataata cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt
    11821 cgaaccggtt actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt
    11881 gaatgagtcg gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc
    11941 gataaatagt ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg
    12001 gcacaatttc cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt
    12061 gactatcttc taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac
    12121 tataaccaaa ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata
    12181 aactaagtac aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg
    12241 cttctttttc tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac
    12301 tgaatccttt tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc
    12361 tgtccgaaaa taagtaggaa agaagaccaa tgagtcctac cagtgttaaa gacactgcaa
    12421 ctagcccgat tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc
    12481 tgtaaatcca attattataa tctgatttca gtactatata cgttttatct ttttctctta
    12541 tcactttcag ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat
    12601 tttttactgt ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt
    12661 ctccactttc accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg
    12721 catatttttc tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct
    12781 tttgccaaaa taaattattt ttgttattta tgtacttctg ttgatacttt tcaatttcct
    12841 cttcggtcag ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta
    12901 aatcataatt attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt
    12961 cagaataagt ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt
    13021 tggtcgaacc atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag
    13081 ttttaacttg cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat
    13141 aggtattctg gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca
    13201 tcggtaaaaa tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt
    13261 aaaataaaag gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc
    13321 ccgctttcaa gaatagcata attaaaaata aaaatccaag taatttttaa cgtgtgcaat
    13381 cttttcatat caaagatcta ttgacttatc gtagttatcg taatatgatg aatgcgttaa
    13441 caaaaagtga aaaagtaagg aaaatgatgg aatggtaacg atcaaagatg tcgctaaagc
    13501 agctggcgtt tctcatacga cagtttctcg tgctttgaat aacagcagtt taataaaaaa
    13561 tgaaacaaaa caaaaaatac gacaactagc taaggaaatg aattattcgc caaactatag
    13621 tgcaaaaggt ttagtgaatc aaaaaaaata tctaatcgga ctatttttct caagtttgca
    13681 taaaggaact tcatcaaatt tcctagcaga ttccattgca ggcatccatt cagtattaga
    13741 cacaaattac agtttatctg ttgaaagtat cgatgttgtt gatttaacag aaatcaacct
    13801 gcaacgctac gatggactga tcgttatgag tcaaagtgat gatgatcagc ctttcatcga
    13861 ctatctcaaa gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca atgatccagc
    13921 ggttatcaac gtcactgccg atgatgcggc cggtgttatg caggcagtag attacggtat
    13981 tggatgcgga catacgaaga tcgcttatat tggcggaaag aaaaattttc gttcgacaaa
    14041 agaacgggaa aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc
    14101 ctatttttta acaggagatt acagcttaga aagcggcctc caaaacatgg aatacctatt
    14161 atcactttct gacctgccta ctctggtttt ttgcggcaac gacgacatag cgattggtgc
    14221 attgagagct gtacatttag ctggcctccg tgttccccag aacatttctc tgatcgggtt
    14281 cgatgattct ccagtcgtct cctatctcaa tccgccgctt accaccgtac gtaagccttt
    14341 gaaagaaatg tgtcaagaag gaacaaagct tttactagat ttgatcgaag gcaggacgat
    14401 cgaacaatct aaaattgagc tctccacctt gttgatcctt cgtgagtcga tcaattactt
    14461 gtaaactaca agtaatttta tataaaaatg ttaacgtgtg cataacacag aaaggaagtt
    14521 tatatgaaaa aactgacaaa agaattagtg aaaaaaaacg aagcacctgt aaaagttctt
    14581 cagtttggtg aaggcaattt tatgcgtgga tttattgatt ggcagattca gcagctgaat
    14641 aaccaacaat tgtttaaagg taatgttgca atcattcaac ctttagaaca aggtcttgga
    14701 aatatgatga accagcaaga taatttatat acggtcattt tacaaggact cagcaatggc
    14761 gaaatcgttg atacttccga gattatcacc gtagtagaac aaatgatcaa tccttatgat
    14821 gaatgggatg cttatctatc tctagcagaa aatgacgact tggcctatat cgtctccaat
    14881 acaacagagg caggaatcca attcgtggag actgataatt taacagatac tcccccacag
    14941 agcctcccgg gcaaactgac agccttttta taccgacgtt tcaaattaaa caaagctggc
    15001 tttaccatca ttccttgcga actgattgac agaaacggcg aacgtctaaa agaaatcatt
    15061 ttacgctacg cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa
    15121 aatgttttct gttgtagttt agttgatcgc atcgtcccag gttatcctcg cgaagatgca
    15181 ggtgttttca atcaacggca taactatgaa gatcaattga tggtcaaagc agaaccattc
    15241 atgctttggg tgatcgaagg gcctaaagaa ttggagaatc aactaccatt caaacaggct
    15301 ggcttgaatg tcatcatcac agatgatatg actccttatc gtgaacgtaa ggtgcatttg
    15361 cttaatggac cgcatacagc gatggtccct cttgctttgt tggcggaatt ggaaactgta
    15421 gaagaagtaa tgaaagatcc tttattctcc gcatatgttg atcagttatt caatctagaa
    15481 ttgattccta tgctttcttt acctaaagat gaattagcca tttatgctga ccaaatcaaa
    15541 gaacgtttct tgaatccttt tgcccatcac aaattagaag cgatttcatt aaatagtgtt
    15601 tcaaagttca gcacccgtct gcttccagtg ttcaagaagt atatcgaaga acaaaatcag
    15661 gtaccaccgt taatcactgt ttcactagct gcgctacttc ttatgtatcg aggcgatcaa
    15721 gtaaagccac atgatgatga aaagacgata agcgaattta ctgatgcttg gtctgataat
    15781 ggaacagcta ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa
    15841 ataccgaatg tgacagatac agttcaagaa atagctgcaa agatcgatca aaaaggtgtc
    15901 cggtcagtat tacaagaaat gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac
    15961 aatgaaatta aatcgacgag acagtgtggt cgtcgcatta acccctattc caaaacaaac
    16021 acaactgacg attgatcagc aaacgatcac cactcttgaa gatatcccgc aaggtcataa
    16081 gattgcattg ggtgatttga aagctggaga caacgtgatc aaatatggct atccaatcgg
    16141 tcatgtgacg acagacgtaa ctgccggtca atggctgcac actcataatg taaaaaccaa
    16201 tctttctggt gaattggatt atcgatatga aaaagatgtc catccaagtc attacctttt
    16261 tgagaaccga acatttcaag gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga
    16321 tttgttttta gtacctaccg ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca
    16381 gttcaaggaa aaacatccag atctgggaca gtttgaccat attacgattt taaagcatcc
    16441 ttatggctgt tcgcagcttg gaaaagatca tcaaaatact cgagaaattt tagcggacgc
    16501 agtaaatcat cccaatgccg gaggagtttt agtatttgga ttaggttgtg agaacaatac
    16561 agttcctgaa ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga aatttctagt
    16621 ggctcaagat gtttacgatg agatcgaaca aggtgtggct ctattagaag agcttttaac
    16681 agcggcggaa aatgaccagc gcatatcagt cccgctctct aagttgaatg tcggtttgaa
    16741 atgcggcggt tctgatggtc tatcgggaat aactgctaat cctttacttg gtgcattttc
    16801 tgactacttg attgctcaag gaggcagcac gatcttaaca gaagtgccag aaatgtttgg
    16861 cgcagaacaa gtattgatgg cacgagcgga aaatgaagaa gtttttgact caatcgttga
    16921 cttgatcaat gacttcaaac agtatttctt gtcttacggg gaaccagttt acgaaaaccc
    16981 atctcctggt aataaagcag gtgggatcac tacattagaa gataagtctt taggctgcac
    17041 gcaaaaatca ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa
    17101 accagggctt agtttattgc aagctcccgg gaatgatctt gtagctgcct ctgctcttgc
    17161 ttcatccgat tgccagttgg tcttgtttac cacaggaaga gggactccgt ttggctcata
    17221 tgtcccaacg gtcaaggtat ctacgaatac aactcttttt gaacgcaaag gtcattggat
    17281 ggattttaat gcaggcgtct tattagaaca accaatggaa gtaatcttag aatcgtttgt
    17341 tcaaaaaatc atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg
    17401 agaaattgcc atttttaaaa acggagtaac actttgattt tcataaaaca gtataaaaag
    17461 aaagaaggaa gatttatgtt tttatctgat gattttttat taaaagacga ttgggcgaaa
    17521 aagctttacc attcctacgc aaaaaacatg cccattattg attatcactg tcacttagat
    17581 ccaaaagaaa tttacgaaaa caacaatttc agcaatctga cagaagcttg gttaggaggc
    17641 gatcattaca aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga
    17701 aatgcttctg attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa aattatcgga
    17761 aatccgcttt acagttggtc acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg
    17821 ataaatgaag aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt ggcagctcct
    17881 gattttcgcc gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat
    17941 gatcccgcag acgacttgca ctatcatcag ttgcttgctc atgaagaacc agatttgaag
    18001 gttctacctt cattccgtcc agataaagca ctcaatattg aaaaagaagg atttaatgca
    18061 tgggtcacac tactggaaga agcagctgac caaacgatca ccacctatca agaactgata
    18121 gaagtcttag ggcagcggat agaatacttc catcaacatg gatgtcgtgt ttccgaccac
    18181 gctttagaca tcttacgcta taaagaagcg gatgaaacag tattagaaga gatttttcaa
    18241 aaagcacgag ctaacaaact attatcttca gatgaaatcg atgcttatcg tacagaaaca
    18301 ctgatacatt tgacccactt ctatcattct cataattgga cgatgcagct gcacatccat
    18361 gcttatcgta actgtaatac acaaatgttg gaaaaattag ggcccgatac cggctatgat
    18421 ggaatgaatg atctttcttt gactattcct ctgcaaaaat tattgaaccg tgcagaagaa
    18481 acggatcagt tgccaaagac gatcctttat tcattgaatc cgaatgacta tcctgcgatt
    18541 ttagctttga tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt aggttctgga
    18601 tggtggtaca acgatactcg agctggtatg cgcgatcaat tgacacaatt tgctgatggt
    18661 agtgctttgg ggaattttgt cggcatgctg acagattcaa gaagtttcct gtcctacact
    18721 agacatgaat atttccgtcg tgttctttgt gaatttttag gcgaaatggt catgcgagga
    18781 gaagcacctg aagatgaaca acttttagga agtctagttc aagcgattag ttatacaaat
    18841 gctaaaaatt actttggctt ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa
    18901 acaggatagt gaaataacgg aatccgttta tccacgtttt agagaagaag acggaccaaa
    18961 tttgctccgt cttcttctct tgctgttctg cttcattgcc tataaacctt tatgaaagaa
    19021 gagataacat gcctactatc catattgccg gtgattctac tgcggcagaa aaagatgaaa
    19081 acaagcgtcc cgagacaggt tggggagaaa aaatctctgc ttatttttca cccgagatcc
    19141 agatcaataa ccaagcaaag aatggacgca gcaccaaatc atttatccag gaaggaaggt
    19201 tagcccgtct agcttcacag tttcagccga atgatttctt atttgtacaa tttggacata
    19261 acgaccagaa aatccaagaa gaaagaggta cgcttccctt cggtagctat ttaaaaaatc
    19321 tgcagatatt ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg
    19381 tgacgcgacg agattactta gaaaacggaa cattgaatcc agatatactt ggagactatc
    19441 caaaagcgat gcgtgcattt gctgagaaac accatattcc tcttttagat gtgttctcaa
    19501 gatcacaaga attgttccag accttctcaa aagaagagac aaaaaaattc tacctgcatc
    19561 ttatacctga tacttgtaaa aattacccag aaggactaaa agacaatacc cactttagtc
    19621 cagaaggcgc tgataaagtt gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc
    19681 ctcttgctaa ttatttacaa aaaggagttt gagatgtttc cattaaattt aggcatccgt
    19741 gcccacgatt tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac
    19801 ttgtcccata tccaatttgc accacaaaaa gcatttcctg aacaacttaa attaactgac
    19861 atgacacttg ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa tatcgaactt
    19921 tccattttag gatgctatat caacctatcc agcaaaaatc aagaagttcg tgcacaagca
    19981 ttggcaactt tcaaacagca aattgctctg tgctctgcct atcaagccaa gatgattgct
    20041 acggaaaccg gtagtgtctc tgtcggttac acaaaagaaa attttactga agaagcttat
    20101 cagatcgcca gaaattctat cattgaatta gtcgctcatg cagaaaattt tggcgtaact
    20161 gttgctgttg aagcagggat caatcatcca atctataact atcagttagc taaacgttta
    20221 atcgatgaag tagcttcacc aaatttaaaa atcattttag attgtgcgaa tttgatgcat
    20281 aaagaaaatc atgctgatca ggaaaaagtg atccgaggtg ccttggacaa tttacatggt
    20341 tatattacag cgcttcatct caaagactat atcatggaaa atggaaagat ccgcatggta
    20401 cctgttggca agggctgttt agatttccgc ccggtactgc attatatcaa atatgagcgt
    20461 cctctaatgt acgccactct tgaagctact cctgagattt atgttcctga agcaatagaa
    20521 cacttgcagt ttatttacca agattgttaa ggagaagatt atggaggaac gaatacagaa
    20581 aagttatcaa aaatatcatg caaatgcttt tcagacattg ctgcatcttt atcagggaaa
    20641 ttattggcgt tttgccatgt caggactctt ttttattatc aaacatcttc catcttggtt
    20701 aatgccgatc gctattgcca atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt
    20761 gcaggccatt tatttcaacg cttttttgat ggcttgtctg attggtcaaa atatattgac
    20821 gaattatttt catgtaaaat accatagcac ttcgatccgt caagtagaag ctggactaag
    20881 agcgaccctt atcaagaaaa tccaagaact atctattcct tatcaaaaag aattgcaatc
    20941 tggacgaatc cagtcaaaga tcatccgtga tgtagaagct atccaaacgt tgtcctctca
    21001 agtatttgtc agcggattaa atatcgctgt caatttattc gtagctttag gtatcacgct
    21061 ttataaaagt cctgttgttt tcatgttttt cttattgact gttcctctcg ccgcattcgt
    21121 tactctcttt ttccgtaaaa ggatcagaca gtccaactca gacttcaggc aagaaatgga
    21181 agagacttct gcaaaagtga tcgaagtgat cgaattagca ccgattgctc gagctcatgg
    21241 gttagaaaaa cacgaaacaa ctcgcttaaa taagcatttt actcaaatct atgaaaaagg
    21301 attccactta gatatcctgc aatctgtttt cggatcagtc agctgggcta gttttcagat
    21361 atttcagctg atttgtttga ttttcactgg gatattagct ttacaagggc gcatccaacc
    21421 cggcgatgtc gtgatgtatc agacgtattt cacgacgatc gtcaattccg tttctggaat
    21481 gatcacgctg attcctacga tttcaaaagg catggaatct atcacttcta ttggcgaact
    21541 gttgattgct ggcgatgtag aaacttatca cggtaagaaa aaaatccaac atttggatgg
    21601 aaattttcgt tttgaggatg tttcttttct ttatccggac tcactcaaac cgattatcga
    21661 tcatttttct ctagaggtga aagctggaga aacaattgct tttgttgggc catctggctc
    21721 tggaaaatct acactcctta acttattgat tggatttatc cagccgactt caggaaaaat
    21781 ttttctagat gatcagcctt tggatgaact ggatatgcga agtgtgcgaa attatttagc
    21841 cgttgttcct caaaccactt tgttgttctc cgcttcgatc aaagagaaca tcacttatgg
    21901 cttgaaaaac gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc aactttcttc
    21961 actgattgac cagcttcctg atggtctgga taccttagta ggcgaacatg gcaacaagct
    22021 gtctggtggc caaaagcaac gtatctccat tgcacgagcg ttgatacgtc agccaaaaat
    22081 cattttgcta gatgaagcaa cctcagcttt agataaccag tcagagaaaa aaatccagca
    22141 agccctgaat tacctgacag aaacacccac tacttttatt gtggctcatc ggctttcaac
    22201 gatcaaagag gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg aaataggtac
    22261 ttacgaaaaa ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa
    22321 aataggagct atgacaagac ttttgtcgta gctcctattt ctgaataaac ggtgttctcc
    22381 tgccaactcc tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa
    22441 ttgttccttg taactcagag ttaaacggta ggctcacgac ctcttggtaa atggtgttca
    22501 agagacagcc tatcggcaat aagaagcaat tttgtcatcg ctcaaggccg aacgtctctt
    22561 tcacaccctc tgttgtttca taaaatagtt ttattcgctg taaaggtcgc gaactggtgt
    22621 catgatgata cggttcaagt cattgatgat tgtactgaag ccttgctctt tttgcatcaa
    22681 gtcattgatt acttcttctg attggatttt cacagccatt tcttgtgctt tcattgcatc
    22741 ttcgtcgctg aactcttgtc cactcatttg tttttcttgt aattcgattt gcatcgcttg
    22801 gaagtctttg aacagtttgt gtgcttcttc gtttgcttgt actttctcgt acgcttcttt
    22861 caacgctttg aattctggta attcacggat ctcacgttcg atttgattcg cgctatcgta
    22921 gatgtttgac atgttatgcc ctcttttctt ttagtagtta ctctctattg taacatgggg
    22981 ttattcgttt tcaaaggggc tactctcctt gatatttccg ccagaggtct ttagccgctt
    23041 caccaattcc ctgaacgcct tctttcacca tatcgccgaa gtttttcagc ccttcttttg
    23101 cttgttcacc atattgattc aagttatctt gccaatttcc agactctcca ttagaatttc
    23161 ctgtgtttcc aacttgatct gcagctacca cttgtcctcc tgtggcatag gcatctgcta
    23221 cactaaatcc tgtttttgaa gtatacggaa ggatgccttc tgccgctgat ttgaagactt
    23281 tcccgacaac atttccactt gtcccttcca aatagtgcag cttgctggat tcttcgtaac
    23341 ccatccatgt cgaaatcact acgtctggcg tgtaaccgat catccattgg tcatttgctt
    23401 ttgtggcatc gaaattagtt tctgtggttc ctgttttccc tgctatcgta taacctgcag
    23461 gattcgcagc agccgctgtt ccatttgaaa atgtaccaag aagcatgctg gtcatttcat
    23521 cggctgtttc ctttgatatg acctgttttg gttttttgtt tgtgttatcg acgatcaccg
    23581 ctccagtcga atcaacgatt ttcgtgataa gatgcggcgt atacatctga ccatcattgg
    23641 caaatgcact gtaagcaccc gccatgatca atggagatac tcctttttcc agtccaccaa
    23701 gggctaagcc ataatactta tcagattttg ctaaaggcag gccgaattct tctgctttat
    23761 catatccttt ttgtaaaccg atctcatgca aaagccatac agtaggcaaa ttcaaacttt
    23821 gtgcaacagc ttgatacatc ggtacttctc cttggtatgt cccgtcaaaa ttatgcgcat
    23881 cgtaataact ttgcggttcg tctttcaaga tactggaagg cttgtatcca gcttccagtg
    23941 ctggtgtata tacgcttaat ggtttgatcg ttgatcctgg tgaacgacgc atctgtgtag
    24001 caaagctgaa tcctctaaag acatgatctc ctcttcgtcc tactagtgct tgtacaccac
    24061 ctgtattcgg atctaaagct actgaagcag attggaccat ggcaccatct gaagcatttg
    24121 ctgggaataa tgcactgttt tgataagtag cttctaattg cttttgataa tcctgatcta
    24181 aagctgtata gattttatag cctttattca tgacatcttc ttcatcaaga ccatactcgt
    24241 taactgcttc atcgatcact gcatcaaaat aataaggata acgataacca gaatctgaat
    24301 tatcgtatgt gtcattaaga tacgcgctga tatctgtctg gctttctgct tgttcttgtt
    24361 cttttgtgat ttttccgttt tcttccatta cacttagtac tgtgtttttc cgatcattcg
    24421 cattttttgg gtgatcgatc ggattataaa taccaggacc tttcagcatt cctgccaaag
    24481 tagcagcctc agacagcgta acattcgttg catctactcc aaaatacttt ctggaagcgt
    24541 cttggacacc ccataccccg ttgccaaaat aggcattgtt aagatacatg gtcaagattt
    24601 cttctttgct gtacttcttt tcgatctcga ttgccaaaaa taattccttc gcttttcgat
    24661 caaacgtttg gtctaatgtc agatacgcat ttttggcaag ctgctgagtg atcgtacttc
    24721 ctccgccccc tgatgttccg ccagacaaga ccattcggac tgctgcacgg gcaatccctt
    24781 tgatatcaaa cccgtgatgc tcgtaaaaat tcctatcttc tgtcgagatt actgcatctt
    24841 ggatataggg agaaatcgcg tttaattcta catatgttcc tttttgggcg tacagtgtgc
    24901 cagcttcttc gtttttctta tcataaatga ctgtcgattg gcttaatcct gattttaacg
    24961 tttcgacatt tgcttgtttt gctaaaataa agaggtagat acttgtcact aagacacaga
    25021 tcaaaccaat cagcaaaatc aatttgttca catgatattt tttccagatg cgctttcgcc
    25081 atcgatggaa ttgtccgaga tatggtctga tccaacgcca aaaactgatc aaagcagctt
    25141 ttatcttttc taaaatgctt tgaaaatcca tttgcttctt ctcctgtcta tttgattcaa
    25201 tcgtcttatt ttaccatagt gaaagagtgt tttcttcctg cttaagttgt agttcaatat
    25261 gtttttttac gttagtgcaa gtagagtaac ataagaatga acagaaaaat gtaagttttt
    25321 tcttaaatat gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa ggagtacact
    25381 tgtgaaaatc acgatcactt tacctatgaa ccatcctgaa acaacaattc gcgacttgct
    25441 tgaaaaggaa tggttagttc ctagaaaggt tcgacacttt ttgcgtatac gtaaaaacgt
    25501 ttggatcaat caacaacctg ctttgttcca tcatgaagta aagggcaatg accagatcac
    25561 attgttattc gaagaaacag attataatta tcaagaagtt caattaggag atgcgaagca
    25621 catccgtgtt ttatatgaag atgagcattt gattatagtg gataagcctg caggaatgaa
    25681 gactcatcca aatgaaccga cagaaaacag taccttactc aatcatttag ctgcttatct
    25741 cgacactaaa cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa caagcggtgc
    25801 cgttttattt gcaaaaaatc catttgtctt gccgatactt ggaagaatgt tggaacaaaa
    25861 aatgatctat cggagatatc aagccatcgt atggggagaa attgccaaag atatgaccat
    25921 caccaataaa attggacgag atcgccatga tcggagaaaa agggtcgtag atgataaaaa
    25981 tggtcagagc gccttgacac atgtcactgt caacaaagta ctttctgaac aaacacaagt
    26041 ctattgtgtt ttggaaaccg gacgtacaca ccagatcaga gttcatttag ctcataaggg
    26101 acatccgatc gttggagacc ctctctacca aaatagaaca gctgaacgac tgatgctcca
    26161 tgcattagaa ctgcacatga tccatccatt tactcaagaa aaaatagtcg gtaaagcttt
    26221 acctggatta tgggaataga aataaaaaag aacagagtct tatcagcaga taatgataag
    26281 actctgttct ttttgtgtaa tttgtctgct tacttttgat gttcgtgttc tgctaacagc
    26341 aaactaccga taatcccact ttcattcggc agtccccaac gaacgatata ttcacttact
    26401 tgaggtgttt ccatataacc acccatcaat tcaacaaatt gctgacggat tttttgaagg
    26461 agatgatcct gattcattac cccaccgcct aaaataatct tttcaggtgc taacgtcaac
    26521 gtatagttga tcagtgcttg agcaatataa taagcctgaa tagcccaaac aggatgatct
    26581 tgcggcagat cttgcccttt gatccctgtg cgtgcctcaa tagacggtcc tgctgctaat
    26641 ccttctaaac aatctttatg ataaggacaa gtaccttcat aatggtcatc tggatggcgt
    26701 ttcacccaaa tatgtcccat ttcaggatgt gcgatccctg aaaaaatttc ttcattgatc
    26761 acgacacctg caccgattcc tgttcccacg gtaaggtaaa tacaactatt ttttccttgt
    26821 gctgctcctt gcttcaattc cccatatgca gcagcattga catccgttgt ccaagcagca
    26881 ggaatattgt agcgttcctt gattgtgcca agaaaatcga attgtgccca cccttttttg
    26941 ggagttgcca aaacgtatcc gtaattatct tttgttttgt cgataccaat cgggccgaat
    27001 gagccaatcc cgattccctt caattcataa cgatcaaaaa attcaaatac ctgatccaat
    27061 gtttcttctg gtgttgtggt agggatactt aatttttcta aaatttctcc accatctgaa
    27121 accgcacaca caaattttgt tccgcctgct tctattccgc catacattcg ttttcctcct
    27181 cgaatccgat tgtccgaatc tcataaggtt ggattattgg gacaaccgct tgcttcgttg
    27241 gttcttcaag cagattcaat atgacagttt ttccattgtc tttagttatt tccaattttt
    27301 ctaaatgact agacatattg aaacctctta ctactacttt atcactaaac ttgctgcgtt
    27361 tcaaagctgt aattgcaaac gtttcacttt ttatcgttaa atattgttgt tttgaaatca
    27421 atgttccttc atgatgtttg atttgttgag tggaaaaagg aatctgtgct gcgtatgcat
    27481 gcaaataagt agagaatttt tcttctggtc catgtagttc aatgctgtaa ttgaaccgat
    27541 gctcacctag acactgtgct tctggtgttg gaaaatatcc ccaatcgcct agttctccca
    27601 cactgcgaag tagcgtcact gcgatttgtc cgtcttctgt tacttcatat tcattcaaac
    27661 caaagtttcc gactgttaca ccgtactctt catcatgaat attgacaaat gcttgctgat
    27721 gttgcggatt cgtggggttt tcccaacttt tagacacatg gtttgggcgc ttcaccactt
    27781 caaaaatact atctgcttcg tgatgttcca catgtaactt tgtaggaaac aagacacgta
    27841 agcgatgatc tttcatctga ttgtcgagag tcgtttcaaa gtcgattttt ttactatctt
    27901 ttctgattgt catgattgtt tttatttgaa ggattcgttt ttctttggat cgttctgctt
    27961 ttcgataacg aaattccaca accatctgct gttctttttc taaacgctcg tctgcagaaa
    28021 cagggatttc catttcctgt agaatactta tttttgctat ttccggtgta tctactaata
    28081 cttttacttc agagttttct ttgttactgg ataggattgg ctgatcacag aaaggttgtt
    28141 taaagatata ctcatttcct atatcgccga tatcttcaaa aatatgcaga ttattcaact
    28201 ttcgtttgtt tttgcgatct tccattgtaa tggtaccatt tttttcaatc gtcaaatgta
    28261 gagggccgtt ttcgatgatt cgtccactct gatgaatcat tgtttccttt tcttctgctt
    28321 tcgcttcccc ttctacaagc gcgaatgttt cccaagaaaa tgctggcatg ttttctaatg
    28381 gtaaagtcac tttgatcatt cttgccatat atggcacacg gaaacggtct ttcggcaaat
    28441 cataaccaaa ttggacagtt tcttcagata aaatagctgg tatagtggcc ccactttttg
    28501 tttctacatg atacttacgt ttaggcaaat tttctaactc ttgatatagt tgttcaggta
    28561 ttccttcagc gaatcttttt ctttctaatt cgatggttat ttctgcttca ccagttttat
    28621 ccattcctgc agtatttaca atgacaaatg gaaagctgtc ttttgggaat ccggtcgtat
    28681 cgattgctgc ggtcagctgt tctaaggaat cttgtgcaag atactttcca acttcatctg
    28741 ctttttcaaa acgaggtatc atttcccgat ggacagaatc gactgaacaa ccgcaaatac
    28801 tgtcgtgagg atgattttgc attaatgtct tccaagcata atctaattga tcatggggat
    28861 agtttccact aacttcataa gccatcgtcg caagtggttc tgtaatattt tctagctgtc
    28921 gctggacctt ggtattccat tgttttaagt aaacccgagc agatgctgta ttggctaaag
    28981 tataccagcc atccgtttct tgacttgtca attctccttc tactgagcct aagtcttctg
    29041 gcacatcttt ttgaactgct tccagatagt cagtaaaatt agaatgaata aactcgtaat
    29101 ctggaaacag ttcatttgcg agatggattg cttttgatat atctttttgt accggttgat
    29161 gatcaacacc attcatcatc aataagtggt tcgtagaggc atattgttct gcatccgcta
    29221 atttttgctt ccaaaaggca agagctgctt ctttttctac tggaatctca ttcccgttgc
    29281 tataccaatt ggcaaatagc agaccaaaga ttgctgtttg atcaggtcct ttccaccaca
    29341 tctctgagta ttgggaagaa tagttttctg cttccaaaac ttgattatca aagccaattg
    29401 gttttacacc acggccaaac gctgctgctg aaataccagc ttgtttcatt aattgaggcg
    29461 tctgtcccat atttccaaat gtatcgggaa aatagcctaa catgacaggg gttccccatt
    29521 tacggctttc ttccatgcca atcagcatat ttctcacgtt cgattccgag ctgataagaa
    29581 aatcatcttg caagatataa aatggtccaa ttctcaattt tccttcattg attgcttgtt
    29641 ggattgcctg tcttttttct ggccttactt gaagatagtc atctaaaata atcgtttgcc
    29701 catccaaatg aaaactgttg aaggacggat caacttcaaa tagctctagt agatcgtcta
    29761 tcaattcaat cagtcgcatg tgatgctgtt cgtaagccat gtaccattca cgatcccagt
    29821 ggctatgaga aatgatatat actttctttt tcatttacga tacctcctac ttttctactc
    29881 taatatcaaa gtaatccatg actaattcgc aaaacatcat atttgcccaa gaaaaccatt
    29941 ctctcgtata attttcagga ttgttcacat cgaatccttc gtgcataagg tgtgttcctg
    30001 cgtcagttgc tacaagtaaa ttcaatatcc gctctttttc agatttgtct gacgttgtca
    30061 ttccttccat agctaatgca atcggccaaa cgtatttttc tggcgtatgg gaggatccaa
    30121 ttccttttgc gtactttcct tcatagaaat atggattttc tttactcaac aatgttcttc
    30181 ttgtttgcag ataatcttcc tcttgttcag aacaatatcc caagtaaggt gcggcgatca
    30241 gattgggcac attactgtca tccatgactg tggcgtttcc cattccatct acttcatatg
    30301 cataaatcgt ttgccctttt tgattaaccg ttcgtccgtg attctgaatc ccctcttgga
    30361 tctctttctt caatttctgt gtttgctgta caatttcttt atcatcgagt atcgtttgaa
    30421 aaatttcttc cacataacct aacacgacca ccgcaaacat attggatggt accagatatc
    30481 cataacggca agtatcatcg ctaggacgga aaccagacca tgtcattcct gtaaaagctg
    30541 tttttgcacc tcgcccgtca ttgatcagcg tatcttcttt tcttgtcgtg tctcgtacaa
    30601 atgtataagg agagtgttca tgatcttgct ctgttttgaa cacgtccaag atttttttga
    30661 taccttcaat aaaagattga ttgaattgat cagtttttcc tgtatttttg taaagaagat
    30721 aggccaactg tattgggtag cataaagaat caatttcata ttttctttcc cagatccaat
    30781 cgttcatttt tgtatgatct gtttggtggc cagcaccgtt agcttcttcg ttgaaggcat
    30841 tcgcatatgg atcgatattg atataaaaaa attgcttttt gactaacccg ctgatcatgt
    30901 cagctaattc ctcatcttct tttgcaatca ctaaataagg acgaacttgt gcagtagagt
    30961 ctcttaacca catcgctgga atgtctcccg tcaataaaaa ggtggttccg tcattttgct
    31021 ttttgactgt tgtcaacaga gtattggcaa atgctgctgt aaaattcttt gcccagtccg
    31081 catggatatc cccacatttt tgtgtgatat cttccataaa tttctgcacc gattttggta
    31141 tatctttata agccactaat aaaaccttct ttctatttga tatatcttta aagattatac
    31201 ttcatacagc caattatgtc tacgctttca aaaaatatct ttttatttac tttttcatcc
    31261 attatggtat tatttgaata tgatatatca ttaagaggtg tttatatgaa tcaaccattg
    31321 taccagacaa tatttaatga tttgcaaacg gctattttaa ctggtgaatt acctgtagac
    31381 agccaactac caactgaaaa agaactttca cgtacctata aagtaagcag aatcacttcc
    31441 aaacgtgcat tgactgaatt agagcaatta ggacttattt atcgcgttcg cgggaaaggc
    31501 agtttcgtta aatccgcttc tccggaacag attcttgctt ctcctacaaa aacacgacgg
    31561 attctgtttc ttcttccata tgtcgctgat ttgtctgttg gtgattttag caagggattg
    31621 aaccctgtga tgcaagataa acagattgat gtcatgatga cgacgcttga ctttctacaa
    31681 aataaaacag caaaagagct aatcaatgaa tttgacggat tgatttatta tgctcttgat
    31741 acagaacaac atcttgactt actttttgag ctttcattga aagaattccc tgtcgtcata
    31801 ttagataaaa aaatctatga gttacctttt cctaccgttc tttccgataa tttccaaggc
    31861 ggtgcgcttg ctgcctctta tttgatcgaa cagggacatt caaaaatcgc ttatttgttt
    31921 ggtacgcaga ctcatccaca atccgttcga cagcgttacc ttggttacct tgaagcattg
    31981 gacaaagctg gtttgacttt ccacacttca ctggatgaca aaagcgcgac attagatgaa
    32041 ttgctttctt atatcactaa gtatgaagta actgcttttg tttgtgaaaa cgatgttgtc
    32101 gctatccaga ccatgaatct cttgagacag catggttatc atatccctga agatttttca
    32161 attatcggat ttgatgatat ccaagcagca gctcttgttg acccgccact gacaaccatc
    32221 gcacaagatt ttgaacagtt aggtaagctt gcgggagatg ctttggtcac ttggttagag
    32281 acaaaagatc gaccatccga tagtaaattt ccagtttcat tcatcaaaag acaatcaaca
    32341 aaggagaaat aaaatgaata tcggtcaaat agatactaga catgcaacag cgaatcagcc
    32401 aaatttctct aatgggaact gccagcctta tactggcgtt ccttttggga tgaattattt
    32461 tgcaccgcaa actacggatc aaaacggcag ctggtggttt catcctgatg accgcgtttt
    32521 tcaaggctac cgtcttaccc atcaaccaag tccttggatg ggtgatttca gtcatatgct
    32581 gtttacgccg atcaatggaa aactacagga gaacaccctt ttccatgctc aaagttctta
    32641 ccgcccagaa gaaagtatat tttgcccgac tcatctgtcg atctgtcaat tacgttatgg
    32701 catctgttct acattgatac ctagcatgta tggcggaatc ttatctattg attacagcaa
    32761 aaatgacagc ggtttacttt tatcctttcc gggacgccat cagctctttg ttatagatcc
    32821 ttacacagtg gaaggaaaaa tcacacattt tgctggttca gaagatcctg atttttcttt
    32881 ttattttata ttgagatttg aatatccttt aacaattgat gagttagctg tttccaacaa
    32941 aaatagcgac tcgatcccgt tgtattttga acagacaaaa aaacaaacta tccgatttgg
    33001 gacatctttt atcagctcgg aacaagcagc ttataacctg gaactagaac aaaaccaaag
    33061 caaagagact taccttgctc acagtcaaga acaatggcag tcctattttg atcgtataca
    33121 gctcacacac catgatcaac aacaattgcg gacgttttat cataatttat atcgggtgtt
    33181 tttatttcca caaacatttt atgaaattga tcctagtggg caaaaaatcc actatgatac
    33241 gactagtcga tctataaaac ctggtgttct ctatacaaat aatgggtttt gggatacgta
    33301 taaaacggtt tatcctcttt attctttgat tgctgtagaa aaatacgaag aaatgctgga
    33361 aggttttttg aacagctata gagaaagtgg ctatttgcca aaatggctgt ctcctgatga
    33421 acgtggactt atgcctggga cattgatcga tgcggtcatt gcagatgctg ctgtaaaaga
    33481 tatccgacga gacttgatgc ctgaatttct aacggcaatg gaaaaaggtg ctacggttca
    33541 aagcaaacat ccaaattatg gacgccaagg aacagaagat tacttgacat atggctatgt
    33601 tcctaatcac taccacgaat cagtcaatca cacattagac tatgcttata gcgatttttg
    33661 tattagccaa gttgccaagg tactagaaga agaggaaatg acgaagaaat atcagcagca
    33721 atcattgaac tatcaaaata tctttgacta tgagtcagga tttatgcgtt caaaagataa
    33781 agatggcaag ttcagagaac cattttcagc taatcgttgg ggacaagatt atgcagaagg
    33841 aagtgcttgg caaagcagct ttgctgttta tcacgatttt tcaggactga tcggaaaata
    33901 tggtggcagt gagagatata aagaaaaact cattgagctg tgcaatcagc ccccaacgtt
    33961 tgacccagga ggatatggtt ttgagatcca cgaaatgagt gagatggccg cgatagaatt
    34021 tggacaatta gctatttcaa accagccaag ttttcactat ccttacttgt tcagttatat
    34081 aggacaacct gaaatggcac agccactgct taaacagttg atgactcaga ccttcgacga
    34141 ttctccaatg ggttaccctg gagatgagga caatggaagt atggccgctt ggtatatctt
    34201 caatagtctg ggattttatc ctgtcacgcc tggaaccggt gaatatgtta tcggtatgcc
    34261 attaattgac aaggctgttt tgcttttgtc aaatggaaag gaattgacga ttgaagcaac
    34321 gcctaatcgg ccgcagcaac aatttatcca taaaatcaaa cggatggaca aagaatatac
    34381 gaaattatat tttacccatc aagatttgct gaaaggcggc acgatctctt accaattagg
    34441 gatcatccct caccctaaga aatatcaagc aaaagatttg cctttttcct tatcaaacta
    34501 taattaacaa gaggttgtga aaaagctgtc ctgcatcaag taataagaac agccaagcaa
    34561 aaatagatcc tcatattttt cgtttggttg gtcttattac cgcagatgca agcttttgaa
    34621 caccgtttat tgagaggttg tgaaatcagc gttctgacct gagtattaga gagacaaatg
    34681 gcaaaatagt tcctcatatt ttgccatttt ttgagctaat accgagggtc agctgattga
    34741 acaccgttta gtggagaggt cgtgaaaaag ctg
//
LOCUS       DACECZ010000014        34563 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.014, whole genome shotgun sequence.
ACCESSION   DACECZ010000014 DACECZ010000000
VERSION     DACECZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.014"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            212..604
                     /locus_tag="IWO35_000710"
     CDS             212..604
                     /locus_tag="IWO35_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0315787.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="IWO35_000711"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="IWO35_000711"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ0315788.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="IWO35_000712"
     CDS             3165..4775
                     /locus_tag="IWO35_000712"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ0315789.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="IWO35_000713"
     CDS             5067..5936
                     /locus_tag="IWO35_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ0315790.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="IWO35_000714"
     CDS             6101..7372
                     /locus_tag="IWO35_000714"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0315791.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /locus_tag="IWO35_000715"
     CDS             7369..8664
                     /locus_tag="IWO35_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ0315792.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="IWO35_000716"
     CDS             8763..9026
                     /locus_tag="IWO35_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ0315793.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="IWO35_000717"
     CDS             9471..9920
                     /locus_tag="IWO35_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0315794.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="IWO35_000718"
     CDS             9977..11395
                     /locus_tag="IWO35_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0315795.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="IWO35_000719"
     CDS             11395..12255
                     /locus_tag="IWO35_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ0315796.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="IWO35_000720"
     CDS             12429..13202
                     /locus_tag="IWO35_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0315797.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="IWO35_000721"
     CDS             13349..13714
                     /locus_tag="IWO35_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ0315798.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="IWO35_000722"
     CDS             complement(13862..15070)
                     /locus_tag="IWO35_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0315799.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="IWO35_000723"
     CDS             complement(15089..15367)
                     /locus_tag="IWO35_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315800.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="IWO35_000724"
     CDS             15560..16216
                     /locus_tag="IWO35_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0315801.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="IWO35_000725"
     CDS             complement(16658..17455)
                     /locus_tag="IWO35_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ0315802.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="IWO35_000726"
     CDS             complement(17452..18636)
                     /locus_tag="IWO35_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0315803.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="IWO35_000727"
     CDS             complement(18661..19908)
                     /locus_tag="IWO35_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ0315804.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="IWO35_000728"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="IWO35_000728"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ0315805.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="IWO35_000729"
     CDS             21888..22202
                     /locus_tag="IWO35_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ0315806.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="IWO35_000730"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="IWO35_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ0315807.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="IWO35_000731"
     CDS             22887..23531
                     /locus_tag="IWO35_000731"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ0315808.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="IWO35_000732"
     CDS             23546..23875
                     /locus_tag="IWO35_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315809.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="IWO35_000733"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="IWO35_000733"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ0315810.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="IWO35_000734"
     CDS             24863..25687
                     /locus_tag="IWO35_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ0315811.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="IWO35_000735"
     CDS             25680..26027
                     /locus_tag="IWO35_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ0315812.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="IWO35_000736"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="IWO35_000736"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ0315813.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="IWO35_000737"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="IWO35_000737"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ0315814.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IWO35_000738"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IWO35_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ0315815.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IWO35_000739"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IWO35_000739"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ0315816.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="IWO35_000740"
     CDS             31684..32451
                     /locus_tag="IWO35_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0315817.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="IWO35_000741"
     CDS             32441..34483
                     /locus_tag="IWO35_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0315818.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11113 a         6125 c         7258 g        10067 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 ttt
//
LOCUS       DACECZ010000015        33178 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.015, whole genome shotgun sequence.
ACCESSION   DACECZ010000015 DACECZ010000000
VERSION     DACECZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.015"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(76..264)
                     /locus_tag="IWO35_000742"
     CDS             complement(76..264)
                     /locus_tag="IWO35_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ0315819.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            534..896
                     /locus_tag="IWO35_000743"
     CDS             534..896
                     /locus_tag="IWO35_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0315820.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            948..2630
                     /locus_tag="IWO35_000744"
     CDS             948..2630
                     /locus_tag="IWO35_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ0315821.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2747..4783
                     /gene="recG"
                     /locus_tag="IWO35_000745"
     CDS             2747..4783
                     /gene="recG"
                     /locus_tag="IWO35_000745"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ0315822.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4832..5833
                     /gene="plsX"
                     /locus_tag="IWO35_000746"
     CDS             4832..5833
                     /gene="plsX"
                     /locus_tag="IWO35_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ0315823.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5955..6197
                     /gene="acpP"
                     /locus_tag="IWO35_000747"
     CDS             5955..6197
                     /gene="acpP"
                     /locus_tag="IWO35_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0315824.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6465..7469
                     /locus_tag="IWO35_000748"
     CDS             6465..7469
                     /locus_tag="IWO35_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0315825.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7470..8414
                     /locus_tag="IWO35_000749"
     CDS             7470..8414
                     /locus_tag="IWO35_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0315826.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8414..9376
                     /locus_tag="IWO35_000750"
     CDS             8414..9376
                     /locus_tag="IWO35_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0315827.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9403..10317
                     /locus_tag="IWO35_000751"
     CDS             9403..10317
                     /locus_tag="IWO35_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0315828.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10343..12124
                     /locus_tag="IWO35_000752"
     CDS             10343..12124
                     /locus_tag="IWO35_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0315829.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12472..13158
                     /locus_tag="IWO35_000753"
     CDS             12472..13158
                     /locus_tag="IWO35_000753"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0315830.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13178..16759
                     /gene="smc"
                     /locus_tag="IWO35_000754"
     CDS             13178..16759
                     /gene="smc"
                     /locus_tag="IWO35_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ0315831.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16768..17577
                     /locus_tag="IWO35_000755"
     CDS             16768..17577
                     /locus_tag="IWO35_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0315832.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17588..18586
                     /gene="ftsY"
                     /locus_tag="IWO35_000756"
     CDS             17588..18586
                     /gene="ftsY"
                     /locus_tag="IWO35_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ0315833.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18773..21040
                     /gene="gshAB"
                     /locus_tag="IWO35_000757"
     CDS             18773..21040
                     /gene="gshAB"
                     /locus_tag="IWO35_000757"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ0315834.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21129..21581)
                     /locus_tag="IWO35_000758"
     CDS             complement(21129..21581)
                     /locus_tag="IWO35_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ0315835.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21731..22681
                     /locus_tag="IWO35_000759"
     CDS             21731..22681
                     /locus_tag="IWO35_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0315836.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22674..23633
                     /locus_tag="IWO35_000760"
     CDS             22674..23633
                     /locus_tag="IWO35_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0315837.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23630..24385
                     /locus_tag="IWO35_000761"
     CDS             23630..24385
                     /locus_tag="IWO35_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0315838.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24408..25361
                     /locus_tag="IWO35_000762"
     CDS             24408..25361
                     /locus_tag="IWO35_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0315839.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(25884..26180)
                     /locus_tag="IWO35_000763"
     CDS             complement(25884..26180)
                     /locus_tag="IWO35_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ0315840.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26535..26891
                     /locus_tag="IWO35_000764"
     CDS             26535..26891
                     /locus_tag="IWO35_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0315841.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            26901..27800
                     /locus_tag="IWO35_000765"
     CDS             26901..27800
                     /locus_tag="IWO35_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAQ0315842.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            28317..29048
                     /locus_tag="IWO35_000766"
     CDS             28317..29048
                     /locus_tag="IWO35_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0315843.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            29193..31220
                     /locus_tag="IWO35_000767"
     CDS             29193..31220
                     /locus_tag="IWO35_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ0315844.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            31328..32425
                     /locus_tag="IWO35_000768"
     CDS             31328..32425
                     /locus_tag="IWO35_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0315845.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            32732..>33178
                     /locus_tag="IWO35_000769"
     CDS             32732..>33178
                     /locus_tag="IWO35_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0315846.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYVSVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT        11115 a         5737 c         7110 g         9216 t
ORIGIN      
        1 tcaaaagtcc cacccttaaa aatttttatt aaataaaaaa ccgttcacta tctgaacggt
       61 ccaagtatca ttcaattaaa cgcgttcaac ttttcctgat ttcaaagcac gagttgacac
      121 ccaaactttt ttaggttttc cgtcgaccat aacgcgaact ttttgtaagt taggtttaac
      181 agtacgtttt gttgagttca tagcgtgtga acggttgttt ccactttttg tcttgcgacc
      241 tgtaaagtaa caaacttttg ccatggtaat ttcctccttt gccttgattt ggagcaatag
      301 aataattcag ctcataccag actaacatat catatagaat gggaatttgc aagtacattt
      361 tcatagatac aattgacaat tgagaagaat aattgtaaat taaacaaggt tatacctgaa
      421 attttgcaag gtttttgagt actttgggta actgcctttt atttcaagtt gcgctatgat
      481 aaaatatgat agaagaaaaa tgggagtttt ttacccgtta aggaggctca ttcatggctg
      541 taaaaattca aacgtcagct ggaaccattg aaattaccaa tgatgtaatt gcaacagttg
      601 ttggtggcgc cgcgacagat gtgttcggtg ttgtaggaat ggccagcaaa aatcaaatta
      661 aagataatat tactgagatt ttgcgtaaag aaaattactc aagaggagta gttgtccgcc
      721 aagaagataa tggtattgct gtagatgttt atacgattat cagctatggt acgaaaatct
      781 ctgaagtttc ccgcaacgtc caagaaaaag tgaaatacaa tctagaggca ttattaggcg
      841 taactgccaa ttcagttaat gtctttgtac aaggtgttcg tgtactgcca gactaggcag
      901 gagactggct ggtaactcag cagtcgatct gaaggaggat tatctaggtg agtgtaacag
      961 aaataagtgc aagccagttc caggaaatgg tccaggcagg tgcgaatcgt ctgcaagtaa
     1021 atgcagaata cgtcaattca ttaaatgtat tccctgtgcc agatggcgat acgggaacaa
     1081 atatgaatct atctatgact agtggagcta aggcagtagt agattcaacc tcagaaaaag
     1141 taggagaatt agcagcaatg ctttcaaaag gattgctgat gggtgcgcgc ggaaattccg
     1201 gcgttatttt gtctcaattg tttcgtggat tttctaagca aattccagat gttgtctcat
     1261 taaatgcttc tgatcttgct gcagccttca ctcatggagt tgaaactgcg tataaagcag
     1321 taatgaaacc agtagaagga acgattttga cagtagctag agaagcagcg aaagctggag
     1381 aaaagaaagc aaaaagtact gatgatgtcg ttgaagtaat gacagctgtt gtaaaaggag
     1441 caaagcgtgc tttagctaaa actccagact tgcttccagt cctaaaagaa gtcggtgtcg
     1501 tagacagtgg agggcaagga ctgctgtttg tttatgaggg ctttttaagt gcattgaatg
     1561 gagaattcca agcggatgaa acatatgaac cttctccagc agaaatggat gatatggtca
     1621 atgcagaaca tcatcgcagt gtccaaggtc aaatggcgac agaagatatc cattttggtt
     1681 actgtacaga aatcatggta aaaatcggag aaggacctac agtcgatagt acattcgatt
     1741 acgaagaatt ccgtaactat ttagatggta taggagattc tttacttgtg gtcaatgatg
     1801 acgagatcat caaagtccac gtgcatacag aaaacccagg agaagtcttg aattatggac
     1861 aaaaattcgg ttcattagtc aaaatcaaag tcgacaacat gcgcttgcag catgagacga
     1921 ttttggaaca cgatgaagaa gtgaacgagt ttgcgaaaaa agaaatacca cgtatcccgt
     1981 atggtatcgt agcgatcgct gctggtaaag gcgtccaaga gctgtttgaa agtttaggcg
     2041 ctaattatgt tatcagtggt ggacaaacaa tgaatccaag tacggaagac attgttaaag
     2101 cgattcaaga agtaaacgca gataaagtga ttgtcttgcc aaacaataag aacatcttca
     2161 tggcagctga tcaagcagca gaagtagcag atatcccagt agcagttgtt ccttcaagaa
     2221 cgatttccca aggattgaca gctatgcttt cattcaatga tcaagcttct ttagaagaaa
     2281 acaaagaaac gatgacagaa atgttgtcta gcgttgtgag cggtcaagtc actcacgcga
     2341 tcagagatac ctcaattgac ggtgtagaga tcaaagaagg agactttttg ggcatgatcg
     2401 atggaaaaat cctgatctct aacccagata tcacagcaac agcgttaagt acactagaac
     2461 aaatgatcaa tgaggacaca gagatcgtaa cgatcttggc aggtgatgaa ggaacagttg
     2521 aacaagcaaa tgaatttgct gagaaactaa cagaaaaaca tcctgatttg gaaatcgaga
     2581 tccaccaagg agatcagcct gtctatcctt atctattttc tgctgaataa tttctgtgtc
     2641 agaatatctt tctaacacag tattcattca taaaaggcct tcggttagtg atcaggtatg
     2701 atcgctgacc aagtcctttt tttgttaaag gagggaggaa aaatttatgc gttctttaga
     2761 tgatccaatc actatgctgg caggagtcgg agaaaaaaga gcagctagtc ttgccagctt
     2821 gggcatccaa acgattgaag acttactgac ttattatcct tttcgctacg aagatattca
     2881 ggaaagaaat ctaaacgaga tccaggatca agaaaaggtt actttgaaag gaatcgtagt
     2941 ctcgcctcct gtgatgaatc gatttggtta taaaaaaagc cgattgcaat ttcgtatgat
     3001 gcaagaacat gatgtgttca acgtttcttt ttttaatcaa ccttatctaa aagaaaaagt
     3061 cattttatcg gaggaaatcg ccgtatacgg taaatgggat gcaaagcgta aagccttgaa
     3121 cgggatgaag attttaggtt ctcaaacaat ggatgacttt tcgccgatat atcacgtcaa
     3181 caaatcaatt cgtcaaacga cattagttga tctaatcaaa aaagggtttc aagaatacgg
     3241 cacattgatt gaagaaaatc tgccaaacaa ccttgtggag aaatatcgac tgcttgaccg
     3301 agcttctgct gtaagaagta tgcattttcc aaaagaccac gaagaaaacc atcaagccaa
     3361 acgacgtgtc gtttttgaag agttcttttt atttcaaatg cgtcttcaag gattgaaaaa
     3421 agcagaaaaa gcagaaacga atggtttgga gatcctttat gatgtccaaa aattgaaaca
     3481 atttactcaa aagctacctt ttgaactgac aaatgcacaa aaacgtgtaa cgaatgaaat
     3541 ctgtcgggat cttcgcagtc cgcagcatat gcagcgactt ttgcagggag atgtaggtag
     3601 cggaaaaaca gtggttgccg cgattgcgtt atacgctacc gttacagcag gtttccaagg
     3661 tgcgttgatg gtgcctacag aaatcttggc acaacaacat atggaaagct tgcaacagct
     3721 gtttgatccc aatgaagtaa ccatcgcatt gattacgggg tcaacgaaag caaaagaacg
     3781 acgtgagctc cttgaaaaac tagagcaagg ggaaattaac gtcgtgatcg gcacacatgc
     3841 attgatccaa gatggagtcg aatttcagaa tttaggtcta gttattacgg atgagcagca
     3901 ccgttttggc gtgaaccagc ggaaagtact tcgtgaaaaa ggctggcgac cagatgtctt
     3961 atttatgaca gcgactccga ttcctagaac attagctatt acagcatatg gtgagatgga
     4021 tgtatctatc attgatgaac ttccagctgg acgtatcccg atcgaaacac gctgggtaag
     4081 aacacctcaa ttggacagtg tactggattg gacgaaaaaa gaactggcaa gaggacatca
     4141 aatgtatgtc atctgtccgc tgattgaaga atcagaagca ttagatgtaa aaaatgcagt
     4201 ggaaatctat gaaaaacttc gtgaactatt tgcaccaaaa tatgaagttg gcttgttaca
     4261 tggtaaaatg aagaatcagg aaaaagaagt aatcatggaa gccttcaaag acaaccagat
     4321 gcagatatta gtttcgacta ctgtaattga agtaggggta aacgtgccga acgcgaccgt
     4381 catgctgatc atggatgcgg atcgatttgg attggcacag ctgcaccaac ttcgtggacg
     4441 tgttggacgc ggaagtgatg cttcctactg tatcttagtt gctaatccta aaaatgaact
     4501 aggtgtggaa cggatgaaaa tcatgacaga gaccaccaat ggttttgtac taagcgaaaa
     4561 agatctggaa ttacgtggtc ctggtgaagt atttggcttc cgtcaatctg gtctgcctca
     4621 atttgcgatt gctgacttgg tgacggatgg aaatgttttg gaggtagcca gagaagaggc
     4681 tgcagccctt tggcaaataa aggattggca gatgcttcct gagtatcagc ctttagcgca
     4741 aagcctccaa atagaaaatc aggataatcg ttatttcgat tagcagactg tgctatactt
     4801 tatactgatg acggaaaaag gagtggaaac aatgaaaatt gccgtagatg caatgggcgg
     4861 agatcacgca cctcaagcaa ttgtagaagg cgtggcttta gcgcagcaag attttccaga
     4921 agtagaattc ttactttacg gaaaagaagc agaaatcaag aaatacttaa caaatgaaaa
     4981 aaacatcacg attatccata cagacgaaaa aatcaatagt gatgatgagc cagtaaaagc
     5041 aattcgacga aaaaagactg catcaatggt ccttgctgca caagcagtga aaaacggtga
     5101 agcagatgct gtattttcag cagggaatac aggcgcgcta ctagcagcag ggttgttcat
     5161 cgtaggacgt atcaaaaata tcgaacgccc tggcttgatg tctaccttgc cagtagtagg
     5221 gaaagaaggt gccggtttcg atatgctgga tcttggcgct aacgcagaaa acaaaccaga
     5281 acatttgttg caatacggta ttttaggttc tttttatgca cgtaaagtcc gaggtatcgg
     5341 acgtcctcgt gtcgcactct taaacaacgg aacagaagca acaaaaggta gtgaagtgac
     5401 gaaaaaagcc tatgaactcc ttcagaatga aacaagcttg aattttattg gaaacgtaga
     5461 agctcgtgag ctactcaatg gtgtggctga tgtagtggtc acagatggat ttacaggcaa
     5521 cgctgttttg aaatctattg aaggaacagc aatgaatatg atgaatctat taaaatctgc
     5581 tattctaaat gaagggatca aaggaaaaat gggcgctatg cttttaaaag acggcttacg
     5641 ttctttgaaa gcagaaatgg attattccaa acatggcggt gccgttttgt ttggcttgaa
     5701 agcaccagtc attaaaactc atggagcaac aggccccgat gctgttcgct atacgatccg
     5761 ccaaatccac acaatgcttg agacagatgt agttggacaa ttagtcgaac aatttgaaaa
     5821 aagcgaagaa tagtatttgg atatctgaag cgatccgttc cattgaaaaa aaggtggaca
     5881 ttcctgaaaa atgccagtaa aattagccat ggtgtaggta attgacaagt gtatgattct
     5941 tggaggtgaa aaagttgact cgcgaagaag tatttaacaa agtagcgaaa attatttcaa
     6001 atcattttga gatagatacc gataaagtga cggatgaatt gaacatcaaa gatgatttaa
     6061 aagctgattc aattagtatt atggaattcg ttttagagct tgaagatgaa ttcggtacgg
     6121 aaatttcaga tgaagacgct gaacaaattg aaacagtcgg tggcgcagta gattatatca
     6181 gcagccatct taaataattt caaataaaag caaagaagga taaaaagctg catgcagctt
     6241 tttatccttc tttttttaat ctttttgaca cgtaaaagtc atttttctga taattctggc
     6301 gataaacaga aattatgttg caaagtaaag cggttttcta tataattatt aataattcat
     6361 tacacaaaga taagaataac ctttgttata aaacgattta ttctgtgtaa tgtccttttt
     6421 tattaagaaa aacttccatt taggaagcag agaggggtat tcgattgtcc gataaccaat
     6481 tattagaagt tcagaaccta catacaggat tccgactcaa agatgactat tacgatgcag
     6541 tagatgacgt ttcatttact ttagacaaaa acgagatttt agcaatcgtt ggggaatctg
     6601 ggtgcgggaa aagtaccttg gctactacga ttatgggatt acttgatcct aataacacaa
     6661 aaattacagg tgagatttta tataacgatc tgaatttaat cgacctgaac gaaacgctct
     6721 ataataaaat ccgcggaaat gatatcggaa tgattttcca agatccgttg tctgcattaa
     6781 atcctctcat gagaatcgaa gaccagatca aagaaggact ttcctatcac acaaagatga
     6841 atgcagaaca gcgtcaagct cgtgcactag aattgctaga acaagtaggg attcctaatc
     6901 ctgcgagagt tggacgccaa tatccccatg aactatcagg cggtatgaga caacgtgtca
     6961 tcatcgcaat cgcaatcgcc tgcaaacctc caatcgtcat tgcagatgaa ccgacgacag
     7021 ccctagatgt aaccatccaa gcacagattc ttgatttact gaaagacctt caggaagaaa
     7081 caaaaacagg gatcatcttg atcactcatg acttaggagt cgtagcagaa atggctgatc
     7141 gagtggcagt gatgtatggc ggacagttcg ttgaagtagc aggtgtaaaa gaactgtttg
     7201 atcatcctaa gcatccttat acacgctctt tactgaactc tataccacaa gaaggaaatc
     7261 atgatgcaga actccatgtg atcgaaggtg tcgttccttc tctgaaaaac atgcctcgta
     7321 aaggttgtcg gtttgcacca cgtattccat ggataccagc aagcgatcat gaagaagagc
     7381 ctatccttca tgaagtcgca ccgaaccact tggtacgttg ctcctgctat aagcatttcc
     7441 actttagaga cgaaaaagga gaggtgtaga tgacagaatt aatcacaatc aaagacctga
     7501 aagttcatta ccctattcgc agtggattct tcaatcgagt aacggatcat gtatatgctg
     7561 tagatggtgt ggattttatt atcgaaaaag gaaagacata tggtttagtg ggagagtctg
     7621 gctctggaaa atctactacg ggaaaagccg ttgtgggatt agaaaaggta acagccggac
     7681 aaatcatgta tgaaggaaaa gatgtaacga agcgaagcaa ccgcaaaaaa atgggttata
     7741 acaaggatgt gcagatgatc ttccaagatt caatgtccag tttgaatcct aaaaagcgag
     7801 tgttggatat cattgctgaa cccatccgaa attttgaacg gttaagcgat caagaagaaa
     7861 agaaaaaggt aaaaggactg ctagatatcg ttgggatgcc ggaagatgcg ctatataaat
     7921 atcctcatga attttcaggc ggacaacgtc aacgtctagg agtagcccgt gctgttgcta
     7981 ccaatccaaa actgatcgta gccgatgaac ctgtatctgc tctggatcta tctgttcaag
     8041 cgcaagtatt gaatttcatg aaacgaatcc agcaagaata cggcctcagc tatttattca
     8101 tttcacatga tttaggtgta gtaaaacaca tgtgcgacaa tatcgcaatt atgtacaaag
     8161 gtcgatttgt agaaatcggt acaagagaag acatttataa tgacccgcga catatctata
     8221 cgaaacgtct gctttcagct attccgcaaa tcgatgtaga aaatagggaa agccacaaag
     8281 aaaatcgacg aagagtagaa caagagtata tccaaaacca aaaagaatac tatgatgcga
     8341 ctggtcgagt ctacgatttg cgtacgatca caactacaca caaagtggcg ttgaaagatg
     8401 gaggtgcaag ctaatgtgga aaacgattct gcgacgtgtt ttattgatga tcccgcaagt
     8461 tattatttta agtgttctga tcttcttatt agcccaagcg atgccggggg atccatttac
     8521 cggtttgatc aatccaaatc aggatccggc agtgatcgaa cagatgcgac aagcagctgg
     8581 cttgaatgac ccatggtatg tccagtactt ccgttggatc ggaaatgctt tgcaaggcga
     8641 ttttggtcaa agtttcttat acaaacgctc tgttgctgac ttgatcggcg aacggatcat
     8701 caacacttta tatctatcga tcttgacagt tgtccttact tatttgattg ctgtgcctct
     8761 ggggatgctt gctggccggt atcaaaattc aatcttggat aagattgtcg taatctacaa
     8821 cttctttagt tttgctgttc ctttatttat cttcggcttg atcatgctat ttgtttttgg
     8881 ttatcgttta ggctggttcc caacaagtgg atcacaaaca ctaggtttgc aaggtggatt
     8941 tatcactcaa tggatagacc gtttgaaata tattctgtta ccatcaatta cccaagcatt
     9001 tttggcgact gccgtcacaa tccaatatct gcgaagtgaa gtaatcgatt cgaaatcgct
     9061 cgattttgtc agaacagccc gttccaaagg ggtaccgacg aataaagtat tcagtcgaca
     9121 tattttccga aatgcagcgt tgccgatggc ttctcaaatg gggtatgaaa tcacttcgtt
     9181 gattgctggt tctgtagtta tcgaaaagat ttttggttat ccgggagtcg gaaaactatt
     9241 tattgattcc atcggccaac gggactatac cgtgatcaca tccttagtat tgattttagg
     9301 aatcgcgacg ctagtgggga cattactttc agatatcatc atgagtatcg ttgacccgcg
     9361 tattcggatt caataaaacc aatcagaaag gagaatggat cgatgagtga aaaaaacaat
     9421 acagcagttc aagaaagtat tcctcctatg gggatacgaa tgattgctcg agaatttaaa
     9481 aaagataaaa ttgctatttt ttcattaggt ttgctagtgg ttttattatt agtgatcttt
     9541 atcggtgcga tgctgaccga tcaagacaaa gtgatgtatg ttagtatctt cgacaaatac
     9601 gctgcaccag gaacagtttc gacacaagga caaaagttca ttctgggagc agatgaaggc
     9661 ggacgtgatg tattaggaca attgatcata ggtgctagaa actctgttac gatcggattt
     9721 gcgatcacga tccttacttc gatcatcggg gttggattag ggatcatctc tggctactac
     9781 ggagggatca ttgacaacat cttgatgcgg atcgttgatt ttatcatgat tctaccgatc
     9841 atgctgatca ttatcgtctt cgtatcgatt atttccagat acaatgtctg gtcctttata
     9901 ttcatcatga gtgcatttta ttgggtagct aaagcccgat tattccgaag caagacatta
     9961 tctgaagcaa gaagagatta tgtcagtgcc tcaaaaacaa tgggaaccaa tgattttaaa
    10021 atcatgttcc gcgaaatcat gccgaatcta agttcattaa tcattactaa tttgacgatg
    10081 aactttgcgg caaacatcgg tatcgagaca actttgactt tcttaggttt cggcttgccg
    10141 gcaaatgtac ctagtttagg tacattgatc ggttatgcaa gtaacggaga agtcttagtg
    10201 aatcggcaat ggatctggtt acccgcatca atccttattt tagttttgat gttgagtatc
    10261 aattatgttg gacaagcatt caagcgttct gcagatgcgc gccaacggtt aggctagtat
    10321 agaagcgaag ggggaaatag gaatgaagaa aacgattttt ggatttgcag cgctgatgtc
    10381 tgctgtcgtt cttgcagcat gcgggggcgg aggtacagat acctctggaa acaactctgg
    10441 aggcaacggc aataacaaag aggaattaac ttttccaatt gcaacaaaaa ataatgatga
    10501 tgcaatcgaa gacggcgaat tagaagctgc ggtcgcaacg gacagtcagt ttaaaggatt
    10561 gttccagtgg gagttttacc aagattcata tgatgcagct tttatggctc cttctcatga
    10621 accgctattt gcaaacgatg acgatttcag aatcacagac gacggagctg caacgcttga
    10681 tctagatgaa gatacgaaaa aagcgacaat caaagtaaaa gacaacgtca aatggtcaga
    10741 tggtgaagat gtaacggcgg acgatgtgat tttcccttac gaaatcattg ggaataaaga
    10801 ttacactggt atccgttatg acgacacttt ccgtaacatc gtaggtatgg aagaatatca
    10861 tgatggaagt gccgacacaa tctccggaat caaaaaaata gacgacaaaa cagttgaaat
    10921 cgaatataaa gaagtaaacc caagcatgct tcaatcgggt ggtggtatct ggtcatatgc
    10981 agcaccaaaa catacgttag aaggtgtagc aatcaaagat atggaatcaa gcgatcaagt
    11041 acgtaaaaat ccagtgactt ttggccctta ttacatgagt aatatcgttg caggagaatc
    11101 agtagaatac ttgccaaacg agcactactg gaatggtacg cctaaattga aaaagatcac
    11161 gatgaaatct gtaccgatgg caagtgcaac tgaagcattg aactctaaat tatatgatat
    11221 gattttccaa atgccaacag atacttacga tacctataaa gaaattgctg gctatacaaa
    11281 cttaggccgt gaacaaactt cttatactta tctaggattc aagttaggta aatggaatgc
    11341 agatacaaag agtgtggaat acaatccaga ttcaaaaatg gctgataaat cattacgtca
    11401 agcaatgggc tatgcattag ataataatgc agtaggtgaa cgtttctatg caggtttgag
    11461 aagcaatgcg acttcattga ttcctccagt attccagtca taccatgatg atgagatgaa
    11521 aggattcaca caagatctag acaaagcgaa aaaactatta gatgatgctg gctacaaaga
    11581 tgtggatgac gatggtttaa gagaagacaa agatggaaat aaactaacaa tcaactttgc
    11641 ttcgatgtcc ggtggggaaa cagcgcaacc attggcagat tactatatcc aacaatggaa
    11701 agaaattggt ttggatgtcc aatacacaac aggtcgtctg atcgaattca attctttcta
    11761 tgataaatta gaaaacgatg atcctgaaat tgatgtttac caagctgctt ggaatacagg
    11821 aactgatcca aatccaagtg gactatgggg accaaatgct gcgttcaact acactcgtta
    11881 tgaatcagaa gaaaatacaa aattgatcaa cgatatcagt tcaagcaaag catttgatga
    11941 agagtaccaa aaagaagcat atcaaaaatg gcaacaatat gcatttgaag aagcatttgc
    12001 gattccaacg ttgttccgta atgaagtatt gccagtcaac gaccgtgtca aagactggga
    12061 ttggtcttat gatgtaccaa acccatgggc aaatgtttct gttacagcag attcacgttc
    12121 ataatcacta aaaatgaccg aaaaagggcg atgtcctttt tcggtttttt gatatttttc
    12181 tgtttatttc tctaatattc aggtcaaggc gctgatttca tgacttctcc aataaacggt
    12241 gttcaaagtc cagtttctcg gtattaagtc aaatttatgc aaaaatatgg aaagccattt
    12301 tcacaaaatt ttttcttcat actcgaaact aaacgaattt ttcacaactt ctcgtcaatc
    12361 actaggatta ttaaagcttt acttaccttt tgaccgaacc atttcaaatt tgacaaaaaa
    12421 ccagtataat aaggaagtat cgaaaaaata tccataaaag gaatgtggaa aatggacaac
    12481 cagctgacaa aagaactaaa agaaaactat aacatcgttt ttcacgatct aaatttgctt
    12541 gaacaagctt ttacccattc atcctatgtg aatgagcatc gcaatctgca attatcggat
    12601 aatgagcgac ttgaattttt aggagatgcc gttttagaac tgatggtttc tcagtattta
    12661 ttccgtttat atcctcattt gccagaagga aaattgacaa aaacacgagc tgcgatcgtt
    12721 cgcgaagaca gcttgtcgaa atttgccaaa gaatgtcact ttgatcaata tgttttgttg
    12781 ggcaagggag aagagaattc aggaggacgt acgcgaccag cattgctttg cgacctattc
    12841 gaagcttttt taggcgcgtt atatcttgac cagggattcg acacgaccca tgcattcatc
    12901 gaaaaagtga tttttcctaa agtcaaagca ggtgcttttt cacatgagat ggatcataaa
    12961 acaaaactac aagaagtctt gcaaaaatca ggtgatgtat caatcgaata ccgattgatc
    13021 aatgaagaag gtccagctca tgaacgagta ttttggatcg aagtttatgt agatgatcaa
    13081 ttgattggca ctggacaagg gaaatcgaaa aaattagcag aacaagctgc ggcggaaaat
    13141 gcattggcag cactgtaaaa gtaagtgagg gaaagctttg tatttgaaac gaattgaaat
    13201 agccggtttt aaatcatttg cagacaagac agtcattgat tttgaaaata gcgtcacagc
    13261 agtggttgga ccaaatggca gcggaaaaag caatattaca gaggctatcc gctgggtatt
    13321 aggagaacaa tctgctaaaa gtctgcgagg cggaaaaatg ccggatatca tctttgccgg
    13381 ttccgatacc cgtaagcagc tgaatattgc agaagtcaca gtgattttag acaatagtga
    13441 tcattatctg ccattggagt acaatgagat cagtgtaacc cgccgttatc gacggactgg
    13501 ggaaagcgag tttttcatca ataaacaaag ctgcagatta aaagatatcc aagaattgtt
    13561 cttagattct ggattaggaa aagaatcgtt ctcaatcatt tctcaaggaa aagtagaagc
    13621 gattttcagt agtaaaccgg aagaccgtcg tgggatcttt gaagaagcag ctggtgtctt
    13681 gaaatacaag cagcggaaaa agaaagcaga acagaagctt tttgaaacag aagacaacct
    13741 cagccgcgtc caagacatca tccacgaatt ggaagaacag ttaactcctt tggccgccca
    13801 gagtgaggca gcaaaggagt ttttacggct aaaagaaaca ctgactcaaa ccgatgtctc
    13861 tttgatggtt gctgaaatca agacagcaaa aaaagattgg gacaacaaac aagcacagct
    13921 tgcgaaattc aatctagaat taggcaaact atctgagtcg atccaagaac aagaatccat
    13981 tttggcgaag cagcgtaaag aaaatgcgca agccgatcgt ctgatcgaaa aaaatcagca
    14041 agtattatta gatctttcgg aaaaactgaa gcagacagaa ggacaaaaag atgttttgca
    14101 ggagcggaca aagcatacac aaaaaagctc gcaagaatat caaacttcat tagcagaagc
    14161 acaaaaaaag gttaaacatt ttgaaaagct tcaagaaagc ttgatgaaag cagcagcaga
    14221 aaaagaaaca gagatccaaa aagctgaagc aaatctcata aaaacacagc aagaattaga
    14281 aaaataccaa aaatcaacga aagaattatt ggcagaatta agagatcaat atgttgattt
    14341 gatgcaagaa caagcagctg taggaaacga actgaagtat ctagaacgtc aatatataca
    14401 agaaacagca aaaagcaagc agacgttagc caagcaatcg gaagtagaag catcagtaga
    14461 ccgattgatc ttacaaaaag aagaattgac acaaaagcag gcgcagctaa aatcatcttt
    14521 aactgaaaca aaagagaagt tagaaatgat ccaacaaaac gggaaaaagt tccaagaagc
    14581 attagcaaaa gaacagccta aaatgtatca attaatgaat caagtccagc agctacgtgc
    14641 tcgtaagaaa agccttcaag aaatccaaga aaactatttt ggtttttacc aaggggttcg
    14701 attggtcttg cagcataaac agcagctttc gggtattgtc ggagcagtgg ctgaactgat
    14761 cgatgttcct gcagatttca cgttagctat cgaagcagct ttaggtgggg cagcccagca
    14821 tgtcatcgtt gaaaatgaaa aagatgctcg gcaagccatc acgtatttga aacagcaaag
    14881 aggaggaaga gcaacattcc ttccactgac gacaatcaaa ccaagacaat taccggctca
    14941 tatattgaca caagcagcag ctgtagaagg gtttataggg attgccagtg aacaagtcag
    15001 ttatcccgat caaatccaaa ctgtggttca aaacctgcta ggaacgatcc ttttagccaa
    15061 agacttaact agtgccaatg caatcgcgca aacgatccgc tatcaatatc gagtggtttc
    15121 cttagaaggt gatgtgatga atgccggagg atccatgaca ggtggagcga ataaacgagg
    15181 aaaccaaggc agtttgtttg tacaaaatca agaattgaaa cagttgacta gtgaatttga
    15241 agaagcagat aaacaactcc aagcacaaga aaaaaaagtt caggagctgc aacaagaaac
    15301 agctcgttta gccgaagaac aagaagtttt gcgtacacga ggcgaacaat tacgttttga
    15361 agaacaagaa gcgacaaatc agctacaaaa catcataaat gaacttgaac gttttgaaaa
    15421 agaaaagcaa atctcaactt ttgagacacg tgaactgcag cagtttatcg aagattacga
    15481 aaagcaaacg aacgaattga aagacaaaca aacagatctt gaatcgcaac gacaaaaaat
    15541 cgacgaagaa atcaaatcgt taagtcaaga aagcgaccag atggaagcca gaagagcaca
    15601 agtacaaagt caaaaagcac aagaacaagc agatcttgct gtcttgaaag agcagtttaa
    15661 tcatctgcag atccagctcc gcggtgcccg agtccaaaaa gcagaggcaa ccgaacgtca
    15721 agaagcgata gaaaagcagt tggcgacatt gacagcagac ttttctgatc atgaagtcac
    15781 agaagaaagt ttggaaaaac aaattaacga attatctgct cagcgagaaa cattaaaagc
    15841 cgagttggca aaagccaaag agcagcgaga tagaaggcaa aaggagatcg ataagcttga
    15901 agcagtcttg gcagagcgta atcgtgagca aaaagcacga ctgtcggaac aatccaaatt
    15961 agaagtccaa aaagaccgag ctgaaatgat tttagataat catttgacct atctgcagtc
    16021 agaatatcag ctgacatttg agaaagcttc ccaagactat caagaaacaa ccgatatcga
    16081 agacagtaga acaaaggttt catccttaaa agaacagatt gaaaaactgg gaccagtaaa
    16141 cttaaattca atcgaacaat acgaacaagt cagtgaaaga catacattct tagcaacaca
    16201 gcgagatgat ctgttagcag cgaaaaacca attgtttgaa acgatggatg agatggatga
    16261 tgaagtgcgg acaagattca aagaagtatt tgaagctatc cgtcaagaat tcaaaattgt
    16321 ctttccaaat atgtttggcg gcggtaaagc tgaattagta ctgacggaac caactgattt
    16381 gctgaaaaca ggtattgaga ttgaagtaca acctcctggc aaaaaactgc aaagtctaag
    16441 tttgctttct ggaggagaac gagcacttac agccatcgcg ttgctctttt ccattatccg
    16501 tgtctgtcct gttccgtttt gtatcttaga tgaggtagaa gcagcgttag atgaagccaa
    16561 cgtgaaacgt tttggtcgtt acttgagcga tttccaagat gacacacaat ttatcgtagt
    16621 cacacaccgc aaaggaacga tggtcgcagc agatgtactg tacggagtga cgatgcagga
    16681 atccggtgta tcaaaaatcg tttctgtacg gatggaagat atcaatgaag aggcgaaaat
    16741 caacgaaacg aaagtgtagg aacgaaaatg atcaaattag tagcaatcga tttggacggt
    16801 acattattaa atgaacaaaa gctgatttca gatgagaata aacaagcatt agcacaagca
    16861 aaacagcaag gggtcaagat cgttctatgt acaggacgcc ccttagctgc gatggcgcat
    16921 tatctgcaag aattaggact ggtagatgaa ggtgactaca gcatcacttt caacggcggt
    16981 ttggtacaaa aaaatgatac tggtgaaatc atggaaaaga aagtgatgga agtatcagat
    17041 atccaacgct tatacaaact ggctcagcaa ttggatctgc cactagatgt tttgtctgac
    17101 aatgtggttt tacagctgcc tagtgcccca aggaagcaat ccttgtacaa tgtgttgaat
    17161 aagctgctgc aattccaacc agcggatata agcgatatta cagatgaatt tgtgcttaat
    17221 aaggcagtaa tcgcttatga ccaagaagaa ttagatccta aaatcaaaga aataccagca
    17281 gaattccatg aaatgtatga gatcatcaaa acaagaagta tgcttttgga atttatgcca
    17341 aaaggtgtta caaaagctta tggtatttct ttattggcaa aagatcttgg tttgaaacaa
    17401 gaagaaatca tggcgatcgg tgatgaggaa aatgatttac cgatgatcca atatgctgga
    17461 ctaggcgtag cgatggagaa tgctgtgcct ttcgtcaaag aagcagcaga tttcgtgaca
    17521 gccagcaatg aagaaaatgg cgtagccaaa gtgatccaaa aatttgtatt agagtagggg
    17581 gagtcaaatg ggattttttg ataaaatcaa gcgtgctttt actggcgaag agaaaacaga
    17641 agaagttgaa gtacaggaga agtacgacaa gggtttagaa aagacgcgta aaacatttgg
    17701 tgaacggatg aatgagttgt ttgccaattt tcgaacagtc gatgaagact tttttgaaga
    17761 attagaagaa acacttatcg gtgcagatgt aggttttgaa acagcgatac gaatcaccga
    17821 agcattacgt caagaagtga aactcagaaa tgcgaaaaaa tcggcagaag tccaaaatgc
    17881 gatcatcgag aaacttgtgg atctctatga agcggaagga atcaatgaag taaatgaact
    17941 aaatatccaa gaagacggac taacagttat tttgtttgtc ggtgttaatg gtgtagggaa
    18001 aacaacaagt attggtaaac ttgcccatca gttcaaaaac gaaggtaaaa aagtattgat
    18061 ggcagcagca gataccttcc gcgctggagc aatcgaccag ttagttgtct ggggcgaacg
    18121 cgctggtgtt gaagttgttc gtggcaatgc aggaggcgat ccagcagctg tcgtattcga
    18181 tgctttggaa agagcaaaga cagagcaggc agatgtttta ctagttgata ctgcaggccg
    18241 actgcaaaat aaagtgaact tgatgaacga attagaaaaa ataaaacgag taatccagcg
    18301 ggaattacca gaagcacccc aagaaacact tcttgtatta gatgcaacca ctggtcaaaa
    18361 cgcgatggtt caagcaaaac aattcaaaga aacaacagat gtgaccggtc ttgttttgac
    18421 aaaattagat ggtacagcaa agggcggtat cgtacttgct atccgtaatg aattgcattt
    18481 accagtgaaa ttggtcggtc ttggtgaagg aatcgatgac ttggagccgt ttgatccaaa
    18541 tgattttgtc gttggattgt tcaaaggatt actaaaagaa gaataaccca tgtctaaaaa
    18601 gcaggagctg tgacaaaagt tgcagctcct tgtttgaatg attgttttta acatagctgt
    18661 gtttttgatg ccttcatttt tatacttatt tgtaagcggt tctcaaacaa tttcgttctt
    18721 tttcttagac tttttgatta taattagatt catatgaatt ggaggtcact taatgatgaa
    18781 ttttaagcaa ttattattgc atgtcaatgc gcgccctttt atcgaccaag ctcgatttgg
    18841 tatagaacgg gaaggacagc gggttgatct tgcaggaaat ctagcaaaaa ccgatcatcc
    18901 agcaatcttt ggcgatcgat cctatcaccc ctatatccaa acagatttta gcgaaacaca
    18961 aacagagatg atcacccctg ttaccgattc tattcccgaa ttatttcagt atctcgctgc
    19021 tgtttatgat gtgactgctc gttctatacc gaaagaagag atgatctggc cattaagtat
    19081 gccacctgcc ttaccggaaa aagacgaaga gatcattatt gcaaaattaa aaaatttcga
    19141 agatgtcttg tatcgacgtt atttagcaaa agaatacggg aagcgaaaac agatggtaag
    19201 cggtattcat ttcaattttg aatttggcga tgagttgcta agaacattgt tcagccatca
    19261 ggaagaattt caagattttt ccgaattcaa aacagaactt tatttgaaaa cagcgagaaa
    19321 ctttatgcgc tatcgctgga tgatcactta tttattcggt gcttcaccaa tgagtgagaa
    19381 gaactacttt ttagatgaat cacatccgca agaacctgtt cgcagtattc gcaacagtgc
    19441 gctgggctat acgaatcatc caaatgtgaa agtttcctat gcttctatga aacagtattt
    19501 agcagatatc gagcgaatga tcgaagaagg gaaactttcg gaggagaaag aattttatac
    19561 accgcttcgt ttccgaggcg gaaagaaagt cgcagattta gcaacaacag gtgttcgtta
    19621 tattgaattg cggaatatcg atttgaatcc ttatgcaaga ttgggtatca atccagagca
    19681 agttcggttc ttacaattat tcctgatgta catgttatgg acagaagaaa aagaagactg
    19741 tgaccaatgg gtagcagaag gtacgactcg aaataacaaa gtagcactag aacaaccatc
    19801 agatcaaaca gaatttcatc aagaaggtag agaaattctt gaaggaatga agcaaatgct
    19861 ggttgaattg gattggttgg attctcttta tttagtagag gaagcattga ctcaaatgga
    19921 tcatcctgaa caaacgttag cagcgaaact ctatcaagaa gcacagctat cgagtcagca
    19981 agaagttgct gtggcattgg gacatcaata ttataaagaa agccatgaac gtccctatca
    20041 gttggccggt tttcgcgaaa tggaactttc tactcagata tttatgttcg atgccatcca
    20101 aaaaggtgtc caagtcaaag tattagatga gtcggatcaa tttttgcgtc tgcaattcca
    20161 agaccatgta gaatatgtga aaaatgcaaa catgacaagt aaggacagct atatcgtccc
    20221 acttatcatg gaaaacaaaa cagtaacgaa aaaagtctta aaagaagctg ggttccgggt
    20281 accaggaggc gcagaatttt catccatgga agaagcggta aaagcttatc cgagatttgc
    20341 cgagcaggca tttgtgatca aacctaaatc aaccaattac gggttaggta tcacgatttt
    20401 caaggaagga gctagtctgg aagattatca agcaggttta gccattgctt ttcgtgaaga
    20461 cagttcggtt ttagtagaag aatttatgcc gggaacggaa tatcgtttct ttgtgatcga
    20521 cggagaagtc caagccatca tgttgcgagt ccctgctaat gttattggtg acagtatccg
    20581 aacagtgaaa gaacttgttg aagaaaaaaa tagtgaccct ttgcgtggaa cgaatcatcg
    20641 tgcaccttta gaattgatcc aattaggtga attagaacaa ttgatgttga aagaacaagg
    20701 gttgacgatc gaatcagttc cccaagccaa tcaaattgtt tatctaaggg agaactcaaa
    20761 tatcagtaca ggtggtgatt cgatcgatat gactgatgaa ttttctgaag cttacaaaaa
    20821 aatcgccgtt tctgcggtag aagcactagg agctaagatt agtggtatcg atttgattat
    20881 accagataaa gaaatcgatc ctacaactga taaaaaagca tatgggatca tcgaagcaaa
    20941 tttcaatcca gccatgcata tgcatgttta tccttttgca ggaaaaggca gaagattgac
    21001 gatgaatgta ttgaaattgt tatatcctga agtattttaa aagaaaaagc ttttgacaga
    21061 ttgtcctatt tgatgtcaat ctgatcaaaa gctttttttg catgtaaaaa tttatgtgtt
    21121 ttttcgtttt aaaataaacg atcccagaag ctttttttct tttttggttt ttctttttca
    21181 gctggcgaag agttttcttc tgggtatttt ttttcgatat caatcacagg ttcatttact
    21241 gcttcttttg ctgttaagag caatccgaat tcctcaggat ttgcagtgac gtaatcgttg
    21301 actacagtga aacggctatt gtttttcgtt gctagctgga tatattggtt ttggacagaa
    21361 aggggtagag ccccattcaa gagtatggaa gcatctggat gttttttgag ttcttttaaa
    21421 aagttttctt tgttttttgg ttggatcatt tcagcaacag tcatactaag atagcagcgt
    21481 tccctgaatg tccctaaata tttatgctgt tcttcagggt tcactaaagg tgtaccataa
    21541 agtcccttat ccaaatggct ttttacatca tcttgtgcca taaaaaatac cctccttctt
    21601 ctctttgtct ttcttacgat tatagcatat tcccaataat acctaaaaca tatgaaaaag
    21661 cggttgcttt tgttcaacga ttcacttaaa ataagaatga aaatcattct caattagaaa
    21721 ggtggattgg atgcgctacg gacgactagt agcattatta gttattttaa gtagtatttc
    21781 attgttcatc ggagtatcga atgtacaatt gtcagaactg ctccagttag atgggcaaag
    21841 cttacaaatc ttgctagtga ctcgtttgcc acgaacgatt tgtttgatcc ttgtgggagc
    21901 gaccagtagt atttgtgggt tgatcatgca acatttgacc caaaataaat ttgtttcacc
    21961 aacgactgcg ggcacgatgg atagtgcgcg tttaggtatt ttagtggcta tgattttcct
    22021 acctggtgca tccttgattg tccgatcctt gacagcattt tgctttgctt ttgcaggaac
    22081 attgcttttt ttatccctta cgcgattgtt cctccagaaa aatcaattga tgcttccgtt
    22141 gattggagtc atgttaggga atatcattgg ttcagttgct actttttttg cttatcaatt
    22201 ccagttggta caaaatatga cttcatggct tcaagggaat tttgcgactg tgatacgagg
    22261 aaattatgag ttattgtatg ctgttgttcc attgttgctc ttggtttggc tatttgctta
    22321 ccgatttacg atcattggtc tgggcgaatc gtttgcagta aatctgggaa tagattacca
    22381 gaagatgcag tttttaggca ttggcttagt atcattggct agtgcagtca tgttgatcat
    22441 ggtaggaagc attcctttta tcggagtgat cattccgaat cttgtctcta gaatatatgg
    22501 agatcaagtg cacaaaacaa ttggcgtaac tgctttagca ggtagtttgt tcttagtatt
    22561 ttgcgatctt gtcgctcgcg tgttgatttt tccttatgaa ataccagtca gtgtgatcgt
    22621 tggaatcatc ggtggggcta tcttccttta tcttctgata aggggacgac gatatgctta
    22681 aaaaaaagcc atctattttt attggtatgc ttgttcttgt tttatttgtt acaggaatgt
    22741 acttgacgat ccatacatat ggaaactggc attttgcatg tgcattgagg agtaagaaaa
    22801 tttttgcatt tatccttgtc tcgctagctg tcagcagtgc cacaatcagc tttcagacaa
    22861 ttacgcataa ccagtttttg actccttcag tattgggact tgagtcgtta tatgtccttg
    22921 tccaaacggt ccttttcttc tttatcggag gagtgacgat gcttcagcag acaggtacag
    22981 tttttttctt attgaacgtt gggctgatgg tcgggctgag tctattgtta tttttctttc
    23041 ttttgaaaaa aatgagagcg aatctttttt tattactgat gtcagggatg attttaggta
    23101 cgctttttgg tagtgtcagt acctttttac aagtcatcat ggatccgaat gaatatgatt
    23161 tgcttcaagg cagattattt gcaagctttg gcaatatcca gagtcagtat cttttgcctg
    23221 cttctttgat cattatcggc tgctggtacc tgttatttcg atatcgacat gtgctagacg
    23281 ttttccatct cggaaatgaa caagcagtaa acctcgggat agacacagat cgttttgaat
    23341 ttttgatttt atgttgtgtc agtcttttga cagggacatc aacggctctt gtgggtccag
    23401 ttactttttt agggtttatt gtcgcaaata tcagttatcg tgtcttttct acttatcgtc
    23461 atggtcagct atttatcgga agctttctgg tagctatgct gtttctgaca ggaggacagc
    23521 tcgcagtaga gcagattttc ggctggaata caacagtgag tgtcgtcgtt gaatttgtcg
    23581 gaggaattta ttttgtcaca aaattgattc gcgaaaggaa gagtatccta tgattgattt
    23641 acaaaaggtc aataaactgt accatggaaa atcagtggtt aaagatgtac agttaacgat
    23701 tcctgaaaag aagttgactg cttttatcgg tccaaatggt gctggaaagt caacggtgtt
    23761 gtcgatgatc agtcgtttga taccaaaaga tacaggtgaa atctatttag accataacga
    23821 agtaaaggct tggaaatcca atgaattagc caaaaaatta tcgatcctac gacaatcgaa
    23881 tgaaataaat gtccaaatca ccgtgaaaga attagtagaa tttggacgat ttccttatac
    23941 gaaaggaaaa ttgacgacaa aagacgagga aatcgttcag gaatcactgg aacaccttgg
    24001 gttagaagaa atagcggatc aatcgattga cactctttca ggcggtcaac tccaacgtgc
    24061 gtatattgca atggtgctag cgcaagacac ggattatatt ttactggatg aacctttgaa
    24121 caatctagat atgaattttg cggttcaaat catgcagatt ttaaaaaatt tagtacaaga
    24181 aatcgggaaa acaattgtta ttgttcttca tgatatcaac tttgctgcaa gttatgcaga
    24241 cgaaattgtt gcaatgaaag atggacagtt atttacgcaa ggaacgaccc aagaaatgat
    24301 aaaaaaagat gtattggatg aattatacga aatggacata cggatttgtg agattgaagg
    24361 ccgcagattc tgtctttatt tctaaaacta acattaggag agaagagatg aaaaaattag
    24421 tagtaggtat cttgattcta gcatcatttg gtttagcagc atgtggaaat agcaacaaca
    24481 ccagtcaggc agacacaaaa aaaagcagca cacaaactga aactacattg acgataacag
    24541 atagtaatgg agaccaaatc gaggtgccaa ataatccaga aaaagtcgtc gtattcgaca
    24601 atggttcttt agatacgatg gatgctcttg gtgttggaga taaggtagtt ggagcagcta
    24661 caagcagcct tcctgagtat ctttcatcct ataaaaaagt agaatcagca ggtggaataa
    24721 aagaaccaga cttagaaaaa attaatcagc ttcagccaga tctaatcatc atttctggcc
    24781 gacaaagaga ttttcaaaaa gatctaagtc aaattgctcc gaccattttc ctttctttag
    24841 atgcgaaaaa tccatgggaa tctttccagc aaaatgtcac tgctttaggg gagatctttg
    24901 gtaagcaaga agaagctaaa actcaattag aggaattatc tagtgcaatc gatcagacga
    24961 aaaagaaagc agaagctact gacaaaaaag cactagtcac acttgtgaat gaagggcaat
    25021 tatcagccta cggatctggt tctagattcg gtttcatcca tgatttattt ggctttgaac
    25081 aagcagatga tcaaatcgaa gcatccactc acggtcaaag tgtttcttat gaatatgtgc
    25141 tggaaaaaaa tccggatatc ttatttgtag tagacagaac aaaagctatt ggtggagacg
    25201 acagcaaaga tgatatttct gccaatgaac tggtagcaca aacgaatgct ggtaaaaatc
    25261 agcagatcat ctcccttgaa ccagatgtct ggtatttaag cgggggcggt ttagaatcaa
    25321 tgaaactgat gatcgaagat gtgaatcaag catttaaata gcataaataa cagaggatta
    25381 aactctcata ctatgtaaca ataatgtttc ttacattctt aaatagcaag agcctatcct
    25441 caaagaagtt atcaaaaacc atccgtaaaa tcgtattgat gattttacgg atggttttta
    25501 tttatttgag aaatcttttt tcaacgtgaa ttcattatct acatcatacg ggttgattct
    25561 agaatgggga gtggcaaaaa atagtgtgat ccattttttc agcatcaaga tcgcctcctt
    25621 tgatttgaat caaaggtaca aagcgaaact tagataaaac ttaaatttag atatttttat
    25681 tttgtaccga tctcttcttt aaacgatgtt ccatctgctg cttctcggca ataagtccac
    25741 tagactagaa aatatgagaa gctattttct gtccagcgtt cttattactc aaagcaaatc
    25801 gcagttgtca caatctcttc taaacaatgt tcaatcagct gaccttcggt attagctcaa
    25861 agcactgatt tcacaacctc tatttaggca tgtgtctcag caagcggttg atagttgatg
    25921 atctgcattt ttcgataaaa atcggcggga agcttactcc accgttttag taattgatag
    25981 aaataactct cttctgcttt gattttatac tcgcttaata tcaatccaaa actattcgtt
    26041 ttttttgaag ctaatttttt ataaatatcc aatgctttgg ttttatcttc attgacaaag
    26101 agtgttttta cttcaatata agaaccctcg atattttgtg tttcttcgat aaaatagatg
    26161 ttttttatat attccttcat agtgattcct ccaaagaagt cttttatttg ataagtttac
    26221 tctaccgaag agttgttaat aatccgtgaa caaaaagtaa ttctttttcc aatagttgtt
    26281 aaaggttttt tattttttat agtatttttt gttatttaag aaaggaataa ggttaaacaa
    26341 ataacaggtt gctttttggg tctttttccg ttcaagaaac tgaacgaaga aaagggaaag
    26401 ttgtctcttt tgtgataaga gaaatcataa taaacgaatg acttactttc caataaattg
    26461 agaacagtca gaaccagaca aattagttgc atctgatgaa taaagtgatt attctatgac
    26521 atgaggtgat acagatgaaa ttagaagtga caaaagaagc acaagaagta ttgaagaata
    26581 aattagaagc agacgatcaa cttttattag atatcgaaaa cggtgacggt ccatttgcag
    26641 aaagccaagt cagttgccaa ttggatacgg catttcgatt gatcattgtc aaaaaagata
    26701 cacaaacaga tctaagtctt tattctgtag tggctgatac accagttgga ccaatcaaaa
    26761 tcaaagacag tgcaatactt tacatggatg atccatccac acttgcttta gaaccaactt
    26821 ataatagttt acaattaaaa ggaccaagcg gcttgagaaa aggcaacttg caagttgttc
    26881 gaaaagacta gagggataaa atgggaaaat tggcgatcat cagcgaccta catgtggaca
    26941 tcaataaatt cggcgaatca gagctgatgc agctttggca agttttgcaa aagcacaaga
    27001 tcactcggct tcatctagcc ggagatacgg ctaataaagt agaccgttgc ctagctgttg
    27061 tcgaattttt cgtcaataaa ggattatcta cgacatttca tttcggcaat cacgagttgg
    27121 cagatgtgcc caatgaacag atgatggacc attttccaga cccacatttt ttaaatgaac
    27181 ggtatattgc tttaaatgag cagaccgttc ttttagggat gaatggctgg tacgactatc
    27241 agttttccga attgaaagat tacgaggcaa tcgtacgaat gaagcgtttg tattggtatg
    27301 atcggttcat ccagcgtggg aaaacagatc tcgaggtcaa tgcagaaatg cttgcagctg
    27361 caaaaaattt gctagaccag ctggaagcaa aaaaacttga ggtgatttta tccactcatt
    27421 tcgttcctaa acgggcattt atcatgtatc aatctgctcc ttatgagcgt tggaacaaat
    27481 taaatgcatt tttaggatca gaatcattcg gagaattact tgaccgttat tcaaatatca
    27541 aacaagtagt ttttggccat acgcatcgtc gctttgaaga taaatccatc ggtcacacga
    27601 tttacagctg cagacctttt ggttattatt atgaatggca gctgacccga gattttgtct
    27661 tgaaagaaca gttgctagac agctatgatc ctatgaaatt acgtgtcctc ttgcgtgccc
    27721 atcacccagc ttttcaggcc tatcaaacac aacatttgca agaggaattt gaacaagcga
    27781 tgacaatcgt tccgtattga gacagcatta gtaatgtgta aaagaaaata ccttgtaaaa
    27841 cataaggata agtaagagca gtagatccat ttatcgtgga tatgctgctt tttttgcgga
    27901 aaataatcag cgaatgttta tttgatcgga tgatttgtaa ctcgcagagt actagatgca
    27961 agcgtgtatt ctttttttga acgataatca caaaaaaaaa tataatatac attttttatt
    28021 taattataat taatattgat tgaagtctag gcagtcaagt cacattttga tagataggag
    28081 ggggacttac gaattatttt aaaggctatg acagctgtat ttctagattg tgtttgcatc
    28141 aagttgcaat aaaaactaga aatcaaatcg ggctgataat catttataac taatcgatac
    28201 ataggaccaa atataggtct atgttctttt atttcataag tatcgtaaaa cagaattttg
    28261 ctatggatta atccaatcga ctgatatgtt tagtaaataa agaaagagga atgtcaatga
    28321 tgaaaaaaga aatcgtatta acagcagcaa tgatgttatt cagcatggta gcaagtacga
    28381 cttttgtttc tgcaactgaa gtatacccaa aggaatataa cacagaagga acgattacat
    28441 ttgaagcagg agatgagggt gtaactcctc ctgttgatcc agagaatcct gatcccaata
    28501 aacctgtcga tccatcagat cctccaagcc ctggaacagg aggggcatta tcgattgatt
    28561 atggatcaaa attcaaattt gggactcaaa aaatttctac tgcggataaa acgtattatg
    28621 cagctgctga tgtgatgaac gatgggtcta gaaaaccgac atatgtccaa gtgacagata
    28681 ggagaagtac cttatctggg tggaaattaa gtgtctctca accagaacag tttaaaactg
    28741 ccagtgggga tgaactagtt ggagcacagc tgaaatttac gaaagggcag gctgtttctt
    28801 tggttgatcc aacatatacg ccgcaaacgg tgaattctga attaacgttg acaccgggag
    28861 gaaacaatac gttagcaatt aatgctaaat caggaacagg tgtggggact tgggtctatc
    28921 gatttggtgc aaatgaaaat gaaaatcaag atgcggtaca gctttcggta cccggtaaat
    28981 ctgtcaagtt agcgcaacaa tattccacta aattagtttg gacattagaa gatacaccta
    29041 acaactagtt tactgaaaag aactgttaaa aataaatacg tagtcatatt gtcttgcgag
    29101 gtaatcgagc aaaaattgtc aaaggctagg ctaaatttgt ttggatacga atagttcatc
    29161 taggaattta acgatcagga ggaaaagata tattgaaaaa gaggaggatc gtgctcattt
    29221 taagcagtct cttagtgact aatatctttc cgatcatagg gtactctttg gaattgaatc
    29281 agttggagca tggaaataga agagacgaag aaagcaaact cacttcgaca ttcgacagtt
    29341 taatagaatc caaccagaca caagaaagca gtgaggattt aagtatttcg agtagtggag
    29401 agaccactga caacgtagaa gctgagggtt cggacaaaca aggactagaa gatgcaccaa
    29461 tttctgcggt tgagctgcct gacgatccca atattatttc tatcgataaa atttttcaaa
    29521 cacctatcgg tactagcacg tccatattgg aaaatgggaa gttactgcag ttgaacccag
    29581 cagaaaggtc tcaaagagga gctatttggt caaataagaa gattagttta ctctcggatt
    29641 ttacatttaa atcttattta tacttaggaa atcaacgaga tagagcggga gatggcatga
    29701 cctttacatt gactggcgat cctagaatgg ctagtaatcc cagtcaagtt attggatcgc
    29761 caggtatggg aattggctca tattctactc gttctggcca accttacatt gcgaatgcgc
    29821 tctcgattga gtttgatacg tataaaaata gtggttcttc tgatcgaatg gatcgagaga
    29881 taactgctga taatagaggg catggacatg tcgcttttgt cacgcctaaa ccaaataata
    29941 ataattattc tggagagcat actggcgtga cggtagctcc tacatttctt tctaatggca
    30001 catggcgctt gttaataatt aaatgggacg cggagcaaag aagtcttact tatgatttgg
    30061 aaggtgtcgg tcgctcttct cggattatac ctgatctcat taggcagttt ggttctacag
    30121 aagtaaattg ggggtttact tcttcaacag gtactttttt tcaagaaaat gctgtcgctg
    30181 tcacgcgact tccatcgaat gtcacatcaa cagctgatgt aaaagtcaac gatagtgatt
    30241 atggggccat cgctgaagct ggaggaaacg ataagataac tatcagaaat acattgaagg
    30301 tcgaggggga ttttcttgaa tataagaaaa tgcagactag tatcgacctc ccacaagaat
    30361 taactattcc cgagcatatc ttgatcaatg gaaatcaaat tgatgccaaa gatatcttga
    30421 taaccggaaa tcgagtcacg ctaaatctga cagattattt tattgctgga gatacgttaa
    30481 taatcgaatt ggagacaact ttgcaggaag taattcctga aaaagtactc actatgcatt
    30541 ttgaatacct tgaaaacggg aatttgattg agcaatcgaa tgaaataaac ctaaagatcg
    30601 caaagctgaa ggagatagat atccatgttt attataaaga taaagacagt cagattgata
    30661 ttgctgaaat gaaaacaatc actgggaaaa taggagagaa gtataaagaa actcctattg
    30721 atattgaagg gtatgttttt gactcggatt cagcgaatac agaaggcatc ttctccgaaa
    30781 atacagagga tatctatttt tattacagaa aaggcaaact ttatctctca actgctcctc
    30841 aatatgtcaa ttttggaaaa cataaaatat ccagtagacc attaagtgaa ttcggacagt
    30901 tttcaggtgg gttgaaaatt atagatgaac gggcttttgg aagttggaaa ttacaattga
    30961 aacaagagaa agttcttaca aatggagatt ttgagcttcc agaagctctt tcctttgtta
    31021 cctctgaaaa tacaacggtt atagggacat ccgctgttac gattttcgaa agtgatcaaa
    31081 agggtgaaag cgatttgtcc tctcttttag attctacagc tcatcgtgga atcagaataa
    31141 atattcctgt agaaaaccaa agaatcggaa cctttgaggg gaaactatct tggatacttg
    31201 cagatgtccc aggaaattaa caggaagaga agaatataac aaaattattg agtttcaaga
    31261 agggcacagg taccataggc tcttgtgtcc cacagagaca agattttgtt atagtcttct
    31321 cttacatatg attaaaggag cgattttctt gagaccaaaa caacaattat ttataacact
    31381 cctagttatt ttaggggcac tttttaacaa tatatcagtg tcagctagtg catttaattt
    31441 ttcagtgacg ccgataactt cagagaatca aatcgacaag agaaaaactt acttcgatct
    31501 tcaacttgat ccggatcagg aagtagaagt aaaagcggaa ttgagaaatg atacagaaaa
    31561 agaagtgaaa atcgatattt ctgtgaatag cgcaaccacc aattcaaatg tgatggttga
    31621 atacggaaaa aacgagattg aaaaagacat gagcctgatc tttgatctgg tagattatgt
    31681 gagctatcca cagatagtga cgctaaaacc taaaagtgtc caaaccgttg cttttcatgt
    31741 caggatgccg aatgagcgat ttgatggtgt attggctggt gggatcacat tccaagaaat
    31801 ccagacggaa aaggaccaaa cagaaactaa ggatcaaagc ttatcgattg agaatgaata
    31861 cacctatatt attgctttgt tgatgcaaca gacactgaac gaagttgctc cgaaccttac
    31921 tcttcatgaa gttaaaccag atcaaatcaa tgcaagaaat gtcatcttag caaacgttca
    31981 aaatgatcaa aaaacctata tcaatcaagt agttattgaa acaaaaatta caaaaaaagg
    32041 acattctgaa gttttatatc aagaggaaaa agaaggattg cagattgcgc cgaatacaaa
    32101 tttttctttt ccaactgctt taaatggtca accgctaacc ccaggtgaat atcaccttac
    32161 aatgactgtt ttaggaaatg aaaatgaaaa tggaaaattt tcgagaaaaa aaggaaatac
    32221 tacgatcaac tataccaatc aatgggtatt tgaaaaagat tttacaatag acggaaaagt
    32281 agccaaggaa ttgaacacga cggatgttac gattaaaaaa gacaataatt ggatttatat
    32341 cttagtagct tcacttttct tattgtttat tttattatgc atacttttgt ttgttttgag
    32401 aaaaaaagaa gagaaagaaa agtaaggtta agataacaaa tagtaaattt ttattattgt
    32461 aggatggttg ggctcttttt ataacaatat aaaatatacc attttattaa taaaaatgca
    32521 tactagtaaa tgatataatt ctaataacag gtttgatcgc gagaaaggcc aaataaaacg
    32581 ctctccaaaa tgttaaaaaa aagacaggac gatgtatatt atacaaacaa gaatcgctga
    32641 caacagtgac tcggatccag gaaatcagtt ttatccccag ctagttggtg aatgcatgga
    32701 aagtaatata cgtagaaaga gctgatgact aatgaattat gagttttttt taagcaaaga
    32761 agatcacaga agttattcat tacttaaata tttagaagca agctcaaatt tatcagagtc
    32821 aatatcgaat gttcaagaag agttgtcgct atcgacattt ctcttgaaaa aaacgatcga
    32881 taagctgaaa catgatttac aaaagttcga tctagagaaa aatttgcgag taaccgtcag
    32941 tgaattggat atatgtctgg aaattaatgg aaaatgttct agcaaagctc tgttatctcg
    33001 ttatataacg gattctttat ctatgaaaat ggtattggct ttctttcagg aaaaatatgt
    33061 ctcagtagaa aattttgcta aacagcatca cgtgagttat tctgtggctt ataaagtatt
    33121 acaaagacta aaaagaaatc taaaaaaata tcaaattttt tttgaaaaga gaaagttg
//
LOCUS       DACECZ010000016        33129 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.016, whole genome shotgun sequence.
ACCESSION   DACECZ010000016 DACECZ010000000
VERSION     DACECZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.016"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1..579)
                     /locus_tag="IWO35_000770"
     CDS             complement(1..579)
                     /locus_tag="IWO35_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0315847.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(656..1186)
                     /locus_tag="IWO35_000771"
     CDS             complement(656..1186)
                     /locus_tag="IWO35_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ0315848.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1190..2050)
                     /gene="rlmB"
                     /locus_tag="IWO35_000772"
     CDS             complement(1190..2050)
                     /gene="rlmB"
                     /locus_tag="IWO35_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ0315849.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2043..2438)
                     /locus_tag="IWO35_000773"
     CDS             complement(2043..2438)
                     /locus_tag="IWO35_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0315850.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2435..3847)
                     /locus_tag="IWO35_000774"
     CDS             complement(2435..3847)
                     /locus_tag="IWO35_000774"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ0315851.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3844..4380)
                     /gene="cysE"
                     /locus_tag="IWO35_000775"
     CDS             complement(3844..4380)
                     /gene="cysE"
                     /locus_tag="IWO35_000775"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ0315852.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(4702..6162)
                     /locus_tag="IWO35_000776"
     CDS             complement(4702..6162)
                     /locus_tag="IWO35_000776"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ0315853.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6332..7489)
                     /locus_tag="IWO35_000777"
     CDS             complement(6332..7489)
                     /locus_tag="IWO35_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ0315854.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7523..8893)
                     /gene="radA"
                     /locus_tag="IWO35_000778"
     CDS             complement(7523..8893)
                     /gene="radA"
                     /locus_tag="IWO35_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ0315855.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8956..9483)
                     /locus_tag="IWO35_000779"
     CDS             complement(8956..9483)
                     /locus_tag="IWO35_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ0315856.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9713..10519
                     /gene="proB"
                     /locus_tag="IWO35_000780"
     CDS             9713..10519
                     /gene="proB"
                     /locus_tag="IWO35_000780"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ0315857.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10516..11763
                     /locus_tag="IWO35_000781"
     CDS             10516..11763
                     /locus_tag="IWO35_000781"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ0315858.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11839..12183
                     /locus_tag="IWO35_000782"
     CDS             11839..12183
                     /locus_tag="IWO35_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315859.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12300..12626)
                     /locus_tag="IWO35_000783"
     CDS             complement(12300..12626)
                     /locus_tag="IWO35_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315860.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13241..14443)
                     /locus_tag="IWO35_000784"
     CDS             complement(13241..14443)
                     /locus_tag="IWO35_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0315861.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14560..15075
                     /locus_tag="IWO35_000785"
     CDS             14560..15075
                     /locus_tag="IWO35_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0315862.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15274..15720)
                     /locus_tag="IWO35_000786"
     CDS             complement(15274..15720)
                     /locus_tag="IWO35_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0315863.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15767..17245)
                     /gene="msr"
                     /locus_tag="IWO35_000787"
     CDS             complement(15767..17245)
                     /gene="msr"
                     /locus_tag="IWO35_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0315864.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17599..18228)
                     /locus_tag="IWO35_000788"
     CDS             complement(17599..18228)
                     /locus_tag="IWO35_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0315865.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18212..18676)
                     /locus_tag="IWO35_000789"
     CDS             complement(18212..18676)
                     /locus_tag="IWO35_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0315866.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19163..20431)
                     /locus_tag="IWO35_000790"
     CDS             complement(19163..20431)
                     /locus_tag="IWO35_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ0315867.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDCLTPMGWRYEGIGFYGY"
     gene            complement(20662..21399)
                     /locus_tag="IWO35_000791"
     CDS             complement(20662..21399)
                     /locus_tag="IWO35_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0315868.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21466..22035)
                     /locus_tag="IWO35_000792"
     CDS             complement(21466..22035)
                     /locus_tag="IWO35_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ0315869.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22163..23035
                     /locus_tag="IWO35_000793"
     CDS             22163..23035
                     /locus_tag="IWO35_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0315870.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23377..24249
                     /locus_tag="IWO35_000794"
     CDS             23377..24249
                     /locus_tag="IWO35_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0315871.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24246..26204
                     /locus_tag="IWO35_000795"
     CDS             24246..26204
                     /locus_tag="IWO35_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0315872.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26536..27387)
                     /locus_tag="IWO35_000796"
     CDS             complement(26536..27387)
                     /locus_tag="IWO35_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0315873.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27510..27977
                     /locus_tag="IWO35_000797"
     CDS             27510..27977
                     /locus_tag="IWO35_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ0315874.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(28240..28722)
                     /locus_tag="IWO35_000798"
     CDS             complement(28240..28722)
                     /locus_tag="IWO35_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0315875.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(28822..29685)
                     /locus_tag="IWO35_000799"
     CDS             complement(28822..29685)
                     /locus_tag="IWO35_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0315876.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            30283..31158
                     /locus_tag="IWO35_000800"
     CDS             30283..31158
                     /locus_tag="IWO35_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315877.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(31303..31455)
                     /locus_tag="IWO35_000801"
                     /pseudo
     CDS             complement(31303..31455)
                     /locus_tag="IWO35_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(31723..33054)
                     /locus_tag="IWO35_000802"
     CDS             complement(31723..33054)
                     /locus_tag="IWO35_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0315878.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT         9654 a         6922 c         5919 g        10634 t
ORIGIN      
        1 ttattcctca ttataaaatt tcaagtgttt ttttattttg agttttactc tcccgtaagc
       61 gctcattact tgcttgtgat ccattgctaa acattcagcc atgtctacaa aatcaacgcc
      121 ttgcatataa taatgaaaaa ttttagcttc tatttttgaa aactggattt cgcaattttc
      181 gattgcttct tttaataaaa gatgctgaat tgctaaaggt tcttgcaatg tgatctctgc
      241 taaaaagtct ggtccttctg tctctatttt ttggtcgatt gagacttcat ctttttgatc
      301 acgacgcttc agcgcgttct gcttcctcaa cagagaatag atttgattgc ttataacgct
      361 tttataataa cttccaaagc taacaccttt gcttttttcg taagttctca atgcctgata
      421 taaagcgatc cttgcttctt gttcccaatc ctcagaaaca taatttctta aataataggt
      481 gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac agggcagcaa atgcttcttc
      541 gtcaccatta cgtgcagcgc gcaataactc ctcagacata aaataattcc tccataaatt
      601 cgtttatgga ggaatcttat catcaatgat tcatcgaact gattagaacg atatgttatt
      661 tattttttct cattaaatcg tccaattttt gggacaattt ttttaactgc tcttcattcc
      721 atggagaatt acgtcggaaa tcaataaaat gcatatcttt attttcttgg gctattgttt
      781 tttccgtttt ttttatcgtc ttatataatt cataagcaga agttcgtaat gctccttggg
      841 aaaagatcat ccactgttct gctaaatcgc ttgtcgccac tgtcacttgt gtcaaacggt
      901 cattcagctg accagcgatt ctctcgatat aactgtcagc cgtttcgtct tctttcgtaa
      961 aaacaacatc caactggtat ttcttataat tttgttggat gcccggaact agttgggcat
     1021 cgaagacaac gatgacttcc attccttcat atttcgcata atttgataaa cgatgtaaaa
     1081 gagcttctct tgcatccgcc attttgtcct gctttttaag ctggacaagt tccggccacg
     1141 aaccaatcat gttgtagccg tctacaatca acagctgttt tttcatggtc tactccccct
     1201 gacgttggga aaaagcctga tacataagca aaccagcagc aacactggca tttaagctct
     1261 ggacgtgccc ggtcatcggg atcgtcagca attcatccat ttcttttttc aatcctggac
     1321 tcattccttt gccttcatta ccaatgacta aagcaattga tcctttggca ttccattttt
     1381 ggaagtttgt gccggtcata tctgtgccaa atacccaaaa tcctgtttct ttgagctgtt
     1441 gcacagcttg taccaaattc gtcacgcgtg caacagggac atgttcgact gccccagtgg
     1501 aagtctttgt tacgataggt gtgattccaa ctgctcggtg ttttggtatg atcacaccat
     1561 cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa attgtgtgga tcttcgatgc
     1621 tatccaaaat caaaaagaag gggtcttctt ttttctcttt tgttcgttca atcagttggt
     1681 caagggataa atattcataa ggtgtaatcg caagaacgac tccttgatgc acgccgtgat
     1741 cgctcaaagt ttctaatttt tgtttaggaa cccatttgac tggcacagct tgttcacgtg
     1801 cgatttcttt cagttcttct actttttctc ctcgtgaatc ttcttgaaga aagagtttat
     1861 tccctcgccc ttgttgtagc gcttctactg cggcatggtg cccaaagacg aaattatctt
     1921 ctaatgcttc ttcattttga tcagaaactg actgatttcg tccagtttgc tttttatttg
     1981 aatatctatt ttgttttttg tcttcaaatc cacgtttcga ttgatgtttg tttgttcttt
     2041 ctttacccat ttttttcacc aactttctga atacaccagc ggatcaattc ttccatccgc
     2101 tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt cgaaccctgt tgcgacccga
     2161 tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt tagcatttct tcctctgcga
     2221 taataaagtt cttcttcttc tgtcaaaaga tgttcttcca gcatttgctg aatcaaaaag
     2281 gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat gcagcttatt gggttttgtt
     2341 tctccttgat caatcaggtg gtcacgaaca tagatttcgt aaatcgcatc tccaacataa
     2401 gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc ttctcttctc catcgggttc
     2461 cttgcggtgt atcttctaat aagatccctt tttctttcaa caaatcacga atttcgtcgc
     2521 ttcggacaaa atcacgattg cttcttgctt gatttcgttc ttcgatcaat tgttcaatct
     2581 cttcatctaa taactcttct gctgtaaata aaataccgaa gatcgaaagc cattgcgaga
     2641 ataaagcttc tccttgtgcc aagacagcgg cagaaacttt cggctggtct agataagtgt
     2701 tcagccactt agcaagctca tagaccactg tgatcccatt tgctgcatta aaatcatcgt
     2761 ccatttcttc gatgaatcgc tgttctaaat tttctaacaa agcgatttta tccgcatcat
     2821 ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa attgtcaaac gtattcttca
     2881 gtcgttgtaa attggtggct gcttctttca tcgtcgtttc actgtagcgg atcggacgac
     2941 ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg atcaacgttt ttgatcattt
     3001 catgtacagt gatgaaattc cctagagatt tactcatttt ttcatcgtct tcgccaatcg
     3061 ttacatagcc attgtgcatc cagtaattag cgaatttttt ccctgttttc gcttcacttt
     3121 gtgcgatttc attttcatga tgaggaaatt ctaaatcttg cccgccacca tggatatcga
     3181 tcgtttctcc taaatgtttt gttgccataa cggaacattc aatatgccaa ccgggacgtc
     3241 ctttgcccca tggtgaatcc caagaaatct cgccaggttt tgcttgtttc cataaagcga
     3301 aatctaatgg gtcttctttt tgctgctgtt cttcccctgt acgctgactt gctccgactt
     3361 ccagttcatc gatggattgg tcacttaatt tcccataatt ttcaaattta cgggtacggt
     3421 aatacacatc acctgaagat tcataagcat agcctttttc aaccaacact tgaataaatg
     3481 ctaaaatatc tggcatatga tccatgactc ttggatgcag tgtagcaggt tcgacattca
     3541 acgctttcgt atcttcttca aatgctcgga tgaaacgatc agcaacttct ggtgctgtaa
     3601 tacctaattc gtttgctgta cggatgattt tatcgtctac atccgtgaaa ttcgatacga
     3661 aatcgacttt atagcctcga tattcaaaat agcgtcgaat tgtatcgaaa gcaatggtgc
     3721 tacgagcatt tccgatatgg atataattat acacagtcgg tccgcagaca tacataccga
     3781 catgtccctt ttgtataggg atgaattctt ctttttttct ggtcaatgta ttgtagattt
     3841 ttatcattct gatctcctca ctttttctcc tttgattcga acgacttttg ctggtactcc
     3901 taccgctgta gcgtcgtctg gaacatcttt caacacaacg gcacctgcac cgattttggc
     3961 gttttttccg atcgttaccg gtcctaatat ctgcgcattc gcagagagca tggctccttg
     4021 tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg ccgcctaatg ttacgccatg
     4081 gaataaaatc acatcgtcct cgatttctgc ggtttcgcca atgacgattc ccattccatg
     4141 gtcgatgaac acgccagttc caatcgttgc tcctggatgg atctcaacac cagttaggaa
     4201 acgccagaat tgagcattga tcttcgccaa taaaaagagc cggtgctgat ataaaaaatg
     4261 agagaaacga tgccaaaaca aggcatgaaa accagggtat gtcaaaacga tctcagctgt
     4321 cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc gctcgggtaa accaactcat
     4381 gactttccac tcctttcgta agaattttat gtaggaaatc atcttcttat aaagaaaaga
     4441 agcgcctttt aaataagttt ccttatcaaa agacgctttc cgcgtggttc cacttttctt
     4501 cataatcaat gattatctct gacagctaac gcctgttata cgttcttggc tactttgttt
     4561 cctataaaac agtttttcct gttttctgtg tttcgccaag accactccca gaggcatttc
     4621 ggcaaccatt cattgatacc ttgcaccacc cggtatctct cttgtaatga tcagctgctt
     4681 acttttttct gttcatcgtg tttagttcag tacttgattt aaatgtgcta atgatttttc
     4741 acgaccaagc aattcgatcg tttcaggcaa ttctggacca tgcatttgtc ctgatacagc
     4801 tacacggatt ggcatgaaaa gattttttcc ttttacgcca gtttcttttt gaacagcttt
     4861 gatggcagct ttgacagttg gagcatctac tacttccatt tgttctagct gtgctttgaa
     4921 tgcgtggaga acagtaggaa ctgtttcacc agcaagtact tcttttgctg cttcattcaa
     4981 tacaggatgt tcattgaaaa atagcgtaga cagatcaacg atttctgcag catagctcat
     5041 ttgaggttga tacaggctaa cgatcttctt cacccattcg attgtctttt catctgggtt
     5101 ttctgctaca cgtccatcag cgattagata aggcagacac atgtcagtaa atgcatctag
     5161 atccattgct ttgatgtaat ggttgtttac ccattgcagt tttttcgcat cgaaagctgc
     5221 gggtgattta cttaggcgtt ttggatcgaa cattttgatc aattcttggt gttcgaagat
     5281 ttcgtcttcg cccacaggtg accagcccaa taatgcaata aagttgaaca ttgcatctgg
     5341 tagatagcct aattcgcgat attgttcgat aaattgcaag attgattcgt cacgtttgct
     5401 tagtttttta cctgtttcac tgttgatgat caatgtcata tgtccgaagg ttggtggtgt
     5461 ccagccaaat gcttcataga tcatcagttg ttttggtgta ttggcaatat ggtcatctcc
     5521 acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc gcaacagcaa aattataagt
     5581 tggcatacca tcgcgttttt ggatgacaaa gtcgccaccg atattatcgg attcgaacga
     5641 aatagcgcct ttcaccatat ctgtaaatgc gtattctgta ttgcgaggca cacggaaacg
     5701 tacaacagat tctagacctt gtgcttcttt ttctgcttgt tcttcaggtg tcaagttcgc
     5761 acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt tgtgcttccc gttctgcttc
     5821 taattcttct tctgtgcaat agcatttgta tgctcgattg ctagcaagca gctgatcaat
     5881 cagcggttga tagatttctt tacgttcaga ttgacggtaa gggccgtatt ctccaggatt
     5941 ttcaggagat tcatcccaat tcattcccaa ccaagcaagg ttttccaact ggcttttttc
     6001 gccatcttcg atattacgtt tttggtcagt atcctcaata cgaataataa agtctccatc
     6061 attgtggcgg gcaaacaaat aattgaataa tgcagttctt gcatttccaa tatgcaggtg
     6121 cccagttgga cttggtgcat agcgtacgcg tactttcgtc attttcttcc tcttctttca
     6181 aactagactt tgactaacag caaaaattcc gtcagttacg gcttctcaaa tatcatttat
     6241 ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa atagtaaagc caactggttg
     6301 aatttcctta ccagttggct gaatttcttt tttattttga ttgtttagtc tttttctctg
     6361 tctctttttt attgtcgatt cctcgtcctg catgtgcagg tttagcaaag atcatgcgtc
     6421 cagcagctgt ttgaagggca cttgttacaa cgacttcgat acgttcattc atgtaatgct
     6481 gtccatcttc tacgacaacc atcgtgccgt catcaaggta ggctactcct tgttggcgtt
     6541 ctgtcccggc ttttactact agaacattca tcgtttcacc agggatgacg actggtttta
     6601 cggcatttgc caacgcattg atattaagaa caggtacatt ttggaattca gagactttat
     6661 tcaagttata gtcattggtt acaatgacac catctaaaag cttagccaat ttgatcaatt
     6721 tgctgtctac ttcactgata tcttcaaagt ctccatcgta catttcgaca gatataccct
     6781 cttctttttg caaggcattt aggatatcta atccgcgacg accacgcaca cgtttcaaac
     6841 tatcccctga gtccgcgatg tattgtagtt cgtaaagaac aaaattaggg atcatcaagg
     6901 ttccttctaa gaatccagtc tttgtaatat cataaatccg accgtcaatg atcacgcttg
     6961 tatccaagat tttatattta tggaaatttt ctcctgattt tcgttctaac agttgactct
     7021 caacttgact agctgtttct tgctcgattt ttttgcttcg tgaaccaaaa actttcttcc
     7081 attcttcgat tcgtgtcgtt cccattctca atcccagata gcctaagatg atcatcagta
     7141 aaatcggcaa tacactattg acgaatggaa tttccatatt gtaaagaggg gtcgagatca
     7201 caacacctaa agtcaatccg atgatcgcac cgatcgcacc gaagagaaga taagtcaagc
     7261 tgacctcact tagtttttgt tctacttgtt tgataatagc tgagatatgt ttggccaaca
     7321 gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct gttggtaaat gtattattca
     7381 gccatccgtt tgactgttga ccgattgcct gccaagctga gggtaaaaat gaaataccca
     7441 agcttgcgcc gacaatgatc atcagcaaag taatgacacg tttttgcatc gtttttcctc
     7501 cgtttcgaca atcatttatt agttagcgga acacacgctt caacgtttca gccaaagttg
     7561 ctactccaac tatctcgatt ccttttggcg cttcccagtt acctaagttg tttttaggta
     7621 ggtatacttt tgtgaagccg agtttttgga cttctttgac tcgttgttca atatgactga
     7681 cacgtctgat ttctccagtc agtccaattt caccgatgaa acattccgtt ggctgtgttc
     7741 ctttttcttt atagcttgaa gcaatactga cagcgatagc taaatcaatc gctggttcat
     7801 ttaattttac cccacctgca gcttttaagt aagcatcttg attctgaaga agcaacccag
     7861 ctcttttttc cagcactgcc atgatcagag atacccgatt gaaatcaaga cctgttgttg
     7921 ttcgtttggc atttccgaac atagtaggtg tgactaatgc ttggatctct accaaaattg
     7981 gacgtgtccc ttccattgcc acaacgatag cagagccagt tgcaccgtct aggcgttctt
     8041 ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc atgctcatgc atttcaaaaa
     8101 tcccaatctc attcgttgag ccaaatcggt tttttactgc tcgcaaaatc cgaaaactat
     8161 gatgttgttc accttcaaaa tataacaccg tatccaccat atgttctaac attcttgggc
     8221 cggcgatcga gccttctttg gtaacatgac cgacgataaa aatggctatc ccatttgttt
     8281 tagcggtttt gagtagttct gcagtggttt ctcttacttg gctgacactt cctgccacac
     8341 ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat gataacataa tcaggggaga
     8401 tatgttcgat ggcacgactg atttcgttca tatctgtttc agcgtataga taaaattctg
     8461 tgttgatact tccgaggcgt tccgcgcgta attttatctg ctctgcgctt tcttcgccgg
     8521 atacatataa gacttttccg cctatggctg ctaattgctg agaaacttgt aacagcaaag
     8581 tggatttccc gatacctgga tcgccaccaa tcaagaccat agaccctggc accacacctc
     8641 cgcctaacac acggttcagt tcttctaatt ttgttttgat ccgaggttct tttttaggga
     8701 cgacgtctgc gatttttgtt ggttttgctt tttctcctgt caaacttgtc cgagttctgc
     8761 ggtccgttgt atcttgttcg atttcttcga ccatcgtatt ccattttccg caatttgggc
     8821 agcgtcccaa gaacttaggc gacacatacc cgcaattttg gcaaacaaat tgtacttttg
     8881 atttttttgc cattgtaccg cctcctttta tcctaatcat gagacattaa atcaagctta
     8941 agtatcttta acgttttatt tactggaaga accaaatccg ccttgacgtt gagcagtcgt
     9001 ttgatcttga tctgctttta aaaacggcaa aaagatccct tggccgatac gctcgccttt
     9061 tttgatcact acatccttga aaccaaagtt ggtgaattgg aacatgatat ggccttcatt
     9121 tgcctcatta ttgtaataat cactgtcaat cacacctact ccgtttgtca acactaaaaa
     9181 ccgcttcaac ggattgcttg aacggttagc aagctgcaaa aattcatctt ctcccatgta
     9241 agctttgatt ccagtaggca cgagggttgg tttcaattgt ttttcatttt ctgatacatc
     9301 ttttttcaat ccatctgcga ctgtttccaa cccctttttc cagcctgctt tccagatact
     9361 tggaatcacg acctcttctg ctgcttcaaa atcatagcca gcagcatgat gtgtagcgcg
     9421 ctgcggtaga ttgatatttt gatctttata tttagaaata acttcaaatc ctctttttct
     9481 caccggtttt cctccctaaa taaactactc ctattctaac atatccgagg ctgtaaaaga
     9541 ctttctttac aaattcttca agaaattctg ctatcttaaa gtaaaccatt aattcgttct
     9601 ttttccaaac gactgcaagt tgtacaaaaa aactattttt tacatgactt tactcgtcaa
     9661 aacaagtaga atagaacgaa attaatgagt ggatatttga ttgaggtgtc acatgcgtaa
     9721 agaaattaaa aatgcaaaac gaattattgt caaagtagga acaagtacat tgatttatcc
     9781 caatggaaat atcaatcttg cggcaatcga tcagttagct tttgtccttt ctgatcttag
     9841 aaatcgggga aaggaagtga tcttagtctc atcaggagct gtaggcgttg gtatgcataa
     9901 attacatttg tcagaacgtc cgactttgat tcctgaacag caagctgcgg cggctgttgg
     9961 tcaagccgaa ctgatgaata tctataatca aagattcagt gtatataatc aacagacggc
    10021 acagatcctc gtgacaagag acgtggtaga ttatcctgaa agtcgtaaaa atgtgaggaa
    10081 tacattggaa cagcttttga ttatggacac gatccctatc gtgaacgaaa acgatactgt
    10141 agcagtggat gaattagatc atctgacaaa atttggcgat aatgatcagc tgtcagccat
    10201 catggcaaaa cttgtcgcgg cagacctttt gatcgtcctt tctgatatcg acgggtttta
    10261 ttcagataat ccgactacaa atcccgaggc tcagatgtat cgctatatcg ataaaatcga
    10321 tgaaaagatt ctggcacaag caggtggtgc tggtagcaca tttggaacag gcggtatgca
    10381 gagtaaatta aaagccgctt ctaggatatt tgaagcaaac agcgcaatga ttttagcaaa
    10441 tggaaaaaat ccggcaatta tttttgatat tcttgcagga aaagaagtag gtactttatt
    10501 taaggaggac ttgaaatgac tgacttgatg caattaggac aacaggcaaa agatagcgcg
    10561 caacaactag cacttatgcc tacaaaccgc aaaaatgagc tacttgttca aatggcacaa
    10621 accatcaaaa agaatcaaca agcgatcatt gaagcaaatc ataaagactt agaaaaagca
    10681 gtcgaaaaca atatcagtga aacgatgcaa gaccgcttgc agctgacacc tgagcgaatc
    10741 aatgccatgg ctacagagat cgaaaaaatt gcttcgttag acgatcctat tggaaaagta
    10801 gatgagatgt ggacgaacac cgacggattg agaatcggca aaaagcgcgt gccactaggc
    10861 gtgatcggca tcatttacga atctcgtcca aatgtcacga cagatgcagc tagtctagca
    10921 ttcaaatcag ggaatgccgt tatccttcgc ggtggcaaag aagcattctt ctcgaatcaa
    10981 ttattagtcc aattattgca gcaagtactg gtcagtgctg gcgaatctcc ttatgccatc
    11041 cagtttgtcg atgatacttc tcatgaaact gcaggacagt tgatgcgatt gacagaatac
    11101 ttagatgtgc tgattcctcg aggcggagca aaattaatcc aacgagtcaa agaacaggca
    11161 acggtaccta tcattgaaac aggtacagga aataatcacg tatatatcga taaagaagca
    11221 cagttatcta tggcagtgaa catcatcgtc aatgcaaaag cttcccgtcc atctgtctgt
    11281 aacgcggcag aaacattgct gatccattct gagatcgcac cttttttctt gccagctatt
    11341 gaaaaagaac tggtagaaca cggggtctct ttacgtgcgg atgctcgtgc gttagaatat
    11401 ctagagacgg ctgctttagc agaagacaca gattgggata ctgaatatct agattatatc
    11461 cttgctgtca aagtggtcga ttctttggat gaagcaattg cacatatcaa tcggcataat
    11521 actaaacatt cagaaacgat cgtgacagat agttatgcgg ctagtcaacg tttcttaaat
    11581 gaagtggacg cggcggctgt ctatgtcaat gcctccactc gttttacaga cggatcggta
    11641 tttggttttg gtgcagagat tggtatctct actcaaaagt tgcatgcccg tggtccaatg
    11701 gggttaaatg aattaacttc cacaaaatat atcatttatg gcgatggaca aatccgcgaa
    11761 taatgtttcg tgtgaaacat tattcccgcc ttttgccttt tttctgtaaa ggttctatcc
    11821 aactagaaag gtgtcaatat gtttgaaatt ttaatgattt tttttattta ccttatttct
    11881 ctgaatatcg ctgcttttct gggagtcggt attttatccc ttttctttca attcaaaaaa
    11941 cgaagcatag ggagtcaacg ggaaaaatgg tcgcagtatt ttgacaaaat cgggcaaaaa
    12001 gggctggtta cacgcttgca cattagttac atggttgcat tatgcttatt agcgacaatc
    12061 aattactatt cttttttcga tcattcaatc gcttatacga ttacgctact gattgctggg
    12121 atttttcact tatcttataa atatcagctg aataaaaacc acttgaatcg gactttccgc
    12181 taaaaaatat ccgtacgata agtagagtgc cttgaagatc ggcaaaaaac tgccggactt
    12241 tttagaggct gtgacaaaga ttttgtcaca gcctcttttt ccatataaac ggcgttcgcc
    12301 taccagctgc ctggtaaatc tgtttcctct tcgttaagaa gcgaaaatcc gtcaaatgtt
    12361 tcgccatcat aataacgaag cagctcatcc atggacaagt tcatcgtttt ttctttttct
    12421 tcttccgctg attcagccag tagctgttcc cgatacacct gatacatacc agctattttt
    12481 tgctgcatcg cttttctagt ttcaccttcc gcattgatta ttggcaattc tttgatatag
    12541 ccacgataaa tcaaccgatc atctgaaaat tcaatgggta attcaacgag tgtcaacccg
    12601 acttcctgaa aatattcttc cagcatctat tatcccccca ttctttttct taaatgaatt
    12661 ttagcttacc tactgaaaat tttccagttt cattattttg agaaaaaaag ggaatgtgac
    12721 aaaaatcgtg tcacatcccc tttttccgaa taaatggtgt tcttctgcca actcctcaat
    12781 cataagtcac aatattcaac aaacatgaga aactgttttt tgaaattgtt ccttgtgatt
    12841 cagagttaaa cgacagtctc acaacctctt ggtaaacggc gttcaatcag ctgaccctcg
    12901 gtattagctc aaaaaacggt aaaatatgag aagctatttt tccgttttgt ttctctaata
    12961 cttaggataa aacgctgatt ttacaacctc tgttaaacgg tgttcaatca gctgtccttc
    13021 ggtattagct caaaaaacgg caaaatatga ggaactattt ttccgtttgt ttctctaata
    13081 ctcaggacaa aacgctgatt ttacaacctc ctgataaacg gtgttcaaaa gcttgcatct
    13141 gcggtaataa gcccaaccaa acgaaaaata tgagaagcta tttttgtttg gctgttctta
    13201 tgacttgatg caggacagct ttttcacaac ctctgatttc ttattcttct tcatttgtag
    13261 caaaataatt acggtgataa atgatacaac atacactgat aagaacaatg gcacctgcaa
    13321 aataaaacgc atagctttca ccaatatgtt caccaatcac acctgcaata aatggtgcta
    13381 aagctccacc gaaaaagcgg acaaaactat agctggacga agcaacgttt cgttgcagac
    13441 ctggtatttc catagcaatc gtcgtcaaca gtgtattgat aattccttgg aaaaatcctg
    13501 ctaacacaat gctgatcggt acaaggactg gcatagaagt tccagctcct aataacagca
    13561 aacaaataat gaataagaca aatgctgcta aaattgtccg ataagtcgta aacttccgct
    13621 caagagccgg tgcaacgaaa atcgaagcaa tagccagaag cacaccccag ccaaaaaata
    13681 caaaaccgac ttctaattca gaatagccaa tcaataaaaa tggcgcatac gcaagtaaag
    13741 taaagaatcc gaaattatat aacaatgcga ttagtccaac tgaacgaagt ttatgattgc
    13801 ttaacgcttt gacaccttcc caaaggccta cttttctctt tggtttctca gtttcaggta
    13861 aaagtaccgc tagggctata aaaccgcaaa acatcaaaaa tgctacccca aaaaatggat
    13921 aacgccatga accactgccc aagaccccac cgactagagg gccaacagcc attccgcagc
    13981 caagtgctgc ttcatacatc atgattgctt tttctgtgtt accaactaaa atactgacga
    14041 tcgttgctaa agcagtagag acaaataaag cattcccgac accccagccg gcacgtaacc
    14101 caatcagcat tccgatattc ccagataatc cgccaagacc agcgaataca acgatgataa
    14161 acaatcctgt catcaatgtt tttttcaaac cgatccgact agataagaaa cctgtaaata
    14221 gcatcaccac accagtgact aacatgtaac tagtaaataa cagggtcgtt tcagctggcg
    14281 tagcgtttaa ctgtgcagca atcgttttta agattgggtc tactagaccc actcccataa
    14341 aagcgatcat acaggtagct gctacaacta gcgctgaact tttccccttt gtttcttcat
    14401 ttgtttgttc ttgtatatct atttgattct ccaatatttc catccctttc tctctattag
    14461 catcatacat gatttatttt acatgtcaat atgcacatgt aaaacgtgga aactttactg
    14521 tgatataata aagaaaataa gacgagaggt aggattttta tgaatacttt agcctatata
    14581 ttattatgcg tgcttttatc caaaccacgt tctgggtatg aattgaagca gctgatcaca
    14641 attttttggg aagcccatca cagtcagatc tatacaacgc ttgccaagtt agaccagcag
    14701 ggttatgtga ccgttttgga caaagacgag cactctcaaa aaaagatcta ccacttgact
    14761 gatgaaggac gtgctcttgt agaggaatgg atcaaagagg aaactcccgc tcctacgcaa
    14821 aaagatgaat ttctagcaaa aatctatgct ttttcgacgc tcgataaaac aacagctact
    14881 ggactgctat tcactagaga acaacagctg aacaaaatca tgcaaaaaaa tcaaaaaaaa
    14941 ttgaatgacc tctcgctaga caagaaacaa gaatttggac gttatgttgt gatccagcgc
    15001 agaattatgc tttgtcagca ggaactccaa tggtgtcagc aagtaagaga acaaatgcag
    15061 cagttttttg agtaagggat aaacagaacc tttctagaaa tatctattga agagcaaatt
    15121 atcttcaata aaagagcaat acagttggaa aagaaaaagc gatccgaacg acaggccaaa
    15181 acctatcgtt cggatcacta tttttctaga actgcattca agagactatg acaaaaagca
    15241 tgccaaggcc ttttttcaaa taacagtgtt ccatcattca ttactttgaa ttgcttttag
    15301 cacacttctt gaaagcgggc caacgatgat caactgcaac ggtaaagcgg caatcacatt
    15361 cattttccaa gcattcatat aagcagagaa taattgatca ggtattcctt gttccatgat
    15421 cacaccgaat aaagacatac atgtcaccat tcctaaaacc atcaagcatg aaatagttaa
    15481 gatcatttgg accttgcttt ctggatttac tggtagttta aaggcaattt ttttcgccag
    15541 cacaccgatg atgaagacat ccaaaatgaa agccaccacg aatccaggca ctagaccaat
    15601 caacaaactt gataaatgaa gatcttgatg cagccataaa ttatacgcgc tcatccccaa
    15661 gaccatcaaa aaacacatca ttgttgtaaa aattattcct tcttttttat ttgtaggcaa
    15721 tctgtaccac tcctttaaat ttgtcacgaa aatcatcgta gcacatttaa aaatttctcg
    15781 taagtacttt ttttattttc tgccctttta tttcataaac ttcatcagcc gtacgctcga
    15841 caaagtaaga atcatgagaa gtaaacaaaa tcgttcccgg atatatttgc attagcttct
    15901 ctaaagcttc gatcgttgcc atatcaataa aattagtcgg ttcatctaac agcaacacat
    15961 tacttggctt agtaaaaagc aaagcaatcg tcagacgggt cgcttctccc ccacttaatg
    16021 tacataacgg tttcgtcaaa gtttcctgag caaaccctaa gtgattcagc aaactacgga
    16081 caagcgattc tgaaaactct gtttgtctca tgacaaaacg aatgatggat tcttcagatg
    16141 tcatttgata acccttttgc tgatatactt gaaagcttac tttcggagag agaaggattc
    16201 cttctccttg tttgcggata tgttctaata aggttgtctt acctgaacca ttttcaccga
    16261 caatcgcgat ttttttacct aaagaaaact gaaaatcacc atttaccagt aatgttctcc
    16321 cacttctttc caattggaca ttttgtccca tgatgggata gcggctgtgg agttcaagag
    16381 attttggtac tggaaaacgg atttgtttca tctgctgcgg cttggttgtt tctcctattt
    16441 tttggagtct tctctctaat gttttcgcat tcttttggat ggctttttgt acactgtctt
    16501 tttgtttaga ggaagacaac cgatccagtc tgatttgttg ctttttcttt ttactggaca
    16561 ctttttccaa cttttccgct tgttttttct tttcttggat cgatttttcc aatcgttttt
    16621 tctctttcat gatcgactct gcttctcgta gctgagtctt tttctccaat tccttttgag
    16681 tgagataggc actataattc cccgaaaact ctcggatttc tccgtctttt acttcccaga
    16741 ttttcgatgc tagctcatct agaaaatagc gatcatgact aacaaaaacc agcgtaccat
    16801 aataataacg aagctcttca atcagtcgat caatactttt tctatctaga tgggttgttg
    16861 gctcgtctag aagcatccct tgttcataag aagaaagaag acgtatcaac tcgatctttc
    16921 gttcttcccc tccgctccat ttccgctctg aagtttcttc cagtcttaac tggaaatgac
    16981 tggctaattc caaatttagg tcctttgctt cagcgagata ggttaattga ggcaggtagt
    17041 tgaaggtgat ctctgtttgg attttcccat gatcgggaaa aagttcaccg gctattagtt
    17101 tgagcaaagt cgatttaccg acaccatttt ctcctatgat ccctatacga tcttgctgat
    17161 agacacgaag agaatcaata gatagttcta gctggttgcc aaagctgact tgtagatttg
    17221 ttatatttac tgctaaattt tccatatgtt ttacactcct atttcatgta aaacaacaat
    17281 tatcgatact aaaaaagagc agaaaaatct gtttgcaaaa aaactgcatc acatcttttc
    17341 ctactctgaa cgaaataact atgactaaat caccaaatct gtcatagctt tcacctattc
    17401 tactgtcgtt cgaaaaaatg gacatactcc atccatgaaa ttgttgttta tacatgtctt
    17461 ttgaatttat ttagttgtta ttcaaaagtt cgaaacgtaa tttcatcgat gcagtcattt
    17521 ttccgacctc cttttttatt aggttaccac acacgattca aaaaaagcaa tagctactga
    17581 atagtaactg aaaacctttt attcgaacat agtgatcttt tctggtgcta aacgaacaga
    17641 ctcgtaacct tcttctaccg cttttcctat tgataaaata ataactggaa cataacggtc
    17701 tttatccaaa ccaaatgcct ctgccaactg atctgcttcg aatccaccaa ttggatttgt
    17761 ttcatagcca tgatcacgcg caactaacat gaattgcata gccgctaagc tagaatcgac
    17821 tttgactaca tcgttcattt cttctcttga aaaactcttg taataaggaa tgatcgcagc
    17881 taattgttga tctctgactt cttttggcat cttccctgat tcgtaagctt ggttgtagat
    17941 ttcttcgcca tattcgtaac attgcatatc gccaaaaatc atgaccattg cagaagaagt
    18001 atcattttgt ctcgtattga aacggatcaa aggacgcaag atttcttttg cttcctcgct
    18061 ttcagcgacc acaaagcgcc atggtttcat attgacagaa gaaggtgcag tcgtcgcttc
    18121 tttgatcatt tccagcattt cttcgtgtgg gattttgtat gtttcatcat atacgcgaac
    18181 ggatcttcta ccagtcataa ttgttgaaaa atcatttttt tgttccatta ttattaacct
    18241 ctttcgtttc tgttgttata ttgcaaagtt gggtattcaa tttatctaaa agtgttagaa
    18301 attgtttcat ttctacatcg ctgaaagccg gggatagcac ggaacctgta tctttatggt
    18361 ccatttcaca atgcttgatt ttcttctggg ctttttctgt tagcgctaca tacacttccc
    18421 ggttgttctg aggatttctc tgacgcgaaa catagccatt tgcttccaat accttcagat
    18481 gacgagtaac tgctccttga tcaatctgca acttttcttg caaactgctt tgtagcaatg
    18541 gattttcttc tttcagtctc atcatgatct cgtatctagt aagacttata ccgatatttt
    18601 cttcaaagat ctggacgatt ttttgatcgg ctaatttgat ttgatataaa aggtgactaa
    18661 tctcttctaa agacataatt tcctcctttc taataattga ctcgtcaacg attgacttgt
    18721 caataataga ataaaaaaag aaaaaagtca aagaatcagc tgtgtaataa aaaaaacgct
    18781 ctcaatagaa aagtatagag aaaagaaatt ttttgtagaa tgaacggaaa aaatgataaa
    18841 gaaagcacaa cgctaaaacc aagattattg attcttatca aactatagaa gatagctctt
    18901 ctgcagtaga taattttaca ccgccaaaaa tttccgaaat cgccttttgc aagaatcccg
    18961 tcgaatatta gaaattggat cagtatgctt aaacagtaat aaaaaagtta tgacaagatg
    19021 ttgtcacaac cttttttgag taaacggtgt tcaaaaactt gcatctgcgg taataagttc
    19081 aaccaaacaa aaaacatgaa aatctgtttt tgtttggctg ttcttactac ttgatgcagg
    19141 gcagcttttt cactacctct ttttaataac cataaaatcc tattccttca tatctccagc
    19201 ccatcggtgt tagacaatct ctttccttag cactcaaggt atagtgatgg gtaccagttt
    19261 tttcatttgg attaaatagt cgataaattg gcttggaacc accagaataa aaacttagac
    19321 cttcataacg ccaaccgatt tttgttaaag agttgatctc agattgtgcc attgtgtaat
    19381 gatggtctcc actgtaagga ttgtataaac ggtagacagg ttgtccgctg tttggtgctt
    19441 gccaagcaat cccttcatat tcccagccta cagttggcag ataatttttt tctccggcat
    19501 tcaatgtata taaatgttct cctgtatgac ggttatacag acgatacatc ggagaagttg
    19561 aagttggtgc tgtatcatac tgtgtcagat tataggtttc gattacattg ttcaatgtag
    19621 aaccataatt aggcgcagtg gcataacgac ctgttagcca agccgtagca tctttgtaag
    19681 agttcgtatt gcttttccat gcaccggaat agtagtaaac gcctggctga aaagaagtcg
    19741 tcttcaatac cgttacgtta tcttgcaatg attctttgta cgaaggatat tttctaaaag
    19801 cggcattcac cgtaatccat tgcccattca catattccga agtaggcatc gtaacagatt
    19861 ggccattata gcttccttta atcccaaata gattgtaatt aggggcagaa gccaaagtac
    19921 tgttccccca gccactttca acgatggctt gagcaatcat gactgatgca tataagtcat
    19981 tggcacttgc caaactttgt gcagttgagg caatactatc aatgaacgct tgttgagaat
    20041 tagcagatga gcgagcataa aaagcatagc cagtatagtt ttcgaattca gacaaatctg
    20101 cttgatgggt atgtgtcaat gtttgcccat ctgtcaattg gttcgtgatt tcttctgcat
    20161 tctctggatt gttctgagct tctgctgatg tactgtcttc ggtttgtgtt tgttctgttg
    20221 tttcagttgt tggttcttcg gttgaaattg tattttctga gacagtagct gactgcaaag
    20281 tactttctgt atcgttttca attcctgttt ctgaagaact cgatgtctga gttgcgacag
    20341 tttctgtcga ttcttcagct agcactgttg tgttggcaac tgctagattt cctaaaagaa
    20401 taacgctaaa taataaattc tttgctttca tcttttttct cctttgaatt atgaaacttt
    20461 tcctaaataa cagtgtaact aaaaaagtaa aagcaagtca atgtccatat ttaaataaca
    20521 tttgtttctt tttaataaat ttgctttttc tgcataaaaa gacgtaaatg aagaatattc
    20581 ttctccattc acgccttaaa ttgatggtcg tttgaataac tatttaacaa gtaattctct
    20641 ctggatatta tccgttatgg attatacttc ctgtgtcgtc ggacggataa tcatgttgct
    20701 gacagacatc cggtctggcg tttctatcgc aaatgctaca gcagaagcta tatcttcaga
    20761 tgttaatccc cattcttttt gcgcttcatg aagttcaaga gcagcctttt tgtcgctgat
    20821 ggtttgatat aattcagttt gaacagctcc tggagagatg atcgtagatt tgatgttatt
    20881 ttgccgttgc tcttgacgta atccttccat gattgcacgg acagcaaatt ttgttccgca
    20941 ataaacggcg gaatcaggat aaacaacatg tcccgccaca gaatcagttg aaatgatatg
    21001 cccagatttt tgtttttcca tgattggaag tacggcggaa attccattta acacacccat
    21061 gatgttgata tcaagcatat tttgccattc atcacggtga ccttcaacta atggagcggt
    21121 tggcatgatt cctgcattat tgtacaatac atcgattcga ccgaactttt ccatcgctaa
    21181 atcgataact gcttggactt gtgcaaaatc tcttacatct gctgtttgaa tatatagctc
    21241 agcttctggt aaagattctt ttattgcttt taggcgatct tctcggcgtg cagctataac
    21301 tagtttagcc cctttttctg ctaatagctt cgttgttgct tcaccgattc cgcttgaagc
    21361 gcccatgatg acaatcactt tttctgataa atcattcatt ctattctcct cctgttttga
    21421 aaaatctctt gcttatactc actatctctt atttcatctt actgttcaaa taatccgttc
    21481 gacaggtttg acgattttgg cagggattcc tgcaacgatg gtattgtctg ggacatcttt
    21541 cgtaacagtt gcattcgctg caacaatggc attctcgcca atcgttacgc ctggcagaat
    21601 agttgctcct gctccgatcc aagcattttt cttgatgtgg attggcttta ctgacaagcc
    21661 tcttcgttct tttggatcgg ttagatggtt caccgtgatg aggttgacac gaggaccaat
    21721 caatactgaa tcttctaaac agatgccacc taagtcaacg aacatcactt gacgattgat
    21781 aaagacattt ttccctatac taatatgttt gccgaaatct gtataaaaag gcagcagaac
    21841 agtcaccgta tcatctactt tgtcactaat gatattttct agccgtttgc ggatatctgc
    21901 tggttcatgg tacccagtat tcagcgctgc taaaggctct tcgttttccg cttggatctg
    21961 atggatcact tcaaagagcg gatccgttac tcgaatctct ttattgacga tttgtttttc
    22021 gatgaggctt gtcattttgc tccctccttt tatccttact ttataacgga cggcttatga
    22081 tgtctaatac ttataaaaca tacttaacta ttccttttaa ctatacttag tggtatgctt
    22141 ttttaaaaac aggagggaaa aaatggatat tcgtatactg acgtattttt tagcagtagc
    22201 tagagaaaaa aatatcagcc gagcagcaaa atctctgcat atcacgcaac ctactctctc
    22261 aaaacaactg aaagatttag aagaagaact tggtgtgaca cttttcactc gaggctcccg
    22321 agagatacag ttgacggatt ctggtcgata tctttattca aaaggaaaag aaatcttgtc
    22381 gttggtagaa aaaacggcga acaatttaac aaatgacgca atcatctctg gagaaatcta
    22441 cattggcgga ggcgaaacta aagcaatctc tttcatttct gatagccttc attcgttgat
    22501 caaggcgcat cctgatatcc agtttcattt atatagcggg aatgcagatg atatcataga
    22561 caaactggat agcggattac ttgattttgg actcgtaatc gaaccgacaa acaaacaaaa
    22621 ataccagtca tttcgactgc cagcccaaga tcgttgggga cttttgatac atcggaatca
    22681 tcctttagca aagcaaacag tcatccatcc gcaggaactg aaaactgttc ctttgatcat
    22741 ttcccggcaa acgtcagtgg acagtcaact agctgaatgg ctaggtttca gcatagatca
    22801 gttatcgatc gtcggaacat ataacttatt gtacaatgct tctttgatgg tcgataacgg
    22861 gacttttggt gcattagctc tagatgggat catcaatact tatgggacgg aaaaagtatt
    22921 catcccactc gatccgccat tacaagcttc actgaatctc atttggaaaa aagaccagcc
    22981 tctttctcct gctgctcgta aatttttaga tgtcctaacg gagaaataca gttaagtaga
    23041 gtttttttcc ttcgctttca gctttttaac aacgaataag ttaagcaaaa gatccaatga
    23101 ttaaataaca taagtttctt aatttgttcc ttccttttat ttctgataat tccagtaaat
    23161 tactataatt ataaaaaaag aattgacaaa acataactgt ctatataaaa ttgattacgt
    23221 taacaataat tcgtttcatg tgactagatc atactaggca tgaaaaatct ctgaggaaca
    23281 tggtttccct aggaggtttt tcgtgtctat ttttgtttgg ccgcaatcaa ctggtctagc
    23341 aaacgtgaaa accaaatctt caaaaagggt gcgcgcatga aaatcaaaaa gatctttaat
    23401 caaaatgcag tattggtaga tgacggtgga caggaaaaag tagctattgg aaaagggatt
    23461 ggatttgaca gaaaaagaaa tgatttgatt tttgatcgtg atgtcgaacg gatatttgtc
    23521 atggaaccag aaggtcaaat caagttgcaa aatttactta atcagattga tgaacgcttt
    23581 ttatttgcag cagaaacgat cattgatcat gcggaaactg ttttgatgga aaagttgaat
    23641 gaacatgtat tgatcgcttt gaccgaccac attgcttttt ctgcggagaa cattaataac
    23701 ggtatcgtca tccgcaacaa acttttaaga gaaatcgaag tgctttacag tgatgaattc
    23761 agtattgctc aatgggcagt agagtatctg acaaaagagc ttgggattcc atatacttat
    23821 gacgaagcag gatatatcgc aatccatatt cacagcggac gaagcggtcg gaacaataac
    23881 catcgcagta tccgtgaagt gacgattgta tctgacatca tccatttagt ggaatcagaa
    23941 ttggaaacag atatccattc agaaccattt tcactgaatt attcaagact ggtgaatcat
    24001 ttacgactgc ttctacaacg aacccatgcg caacaatatg ctgttttaga cacggaaatc
    24061 gtagaaatgg tcaaacgaaa atacccagaa agctataaga tttctaaaaa aatccgtgtg
    24121 ttgctgacca aagaatacca gctcgcgatc acgaaagaag aactgggtta cttggccatc
    24181 catatcgagc gtttaagaag cgcaatcgta caaacaaatg ttaacaatca tgaggaggaa
    24241 aaaaattgaa agcatatcta caacgaatgg gacgttccct tcaacttcca gttgcggtct
    24301 tacctgctgc tgctttacta gtagggatcg gaaactggtg ggcatcttat agtaacgaca
    24361 ttgtcgctca ttttttacaa gctggcggta acgcagtttt aggtcaatta cctttgctgt
    24421 ttgccgttgg gttagcacta gggatgtcag tggacaaatc aggtgctgct gccttggcag
    24481 gtttggtcgc atttgaagtt ccagtaaatg ttttaaaaac tgattcagta gctactttat
    24541 tgaatatcaa accagaatca gttgatccat cttttgctca gatcagtaac gtatttattg
    24601 gtattattgc cggattgatt gcggcagctc tttataatcg tttccacgaa gcaaaattac
    24661 cgatggcttt atctttcttt agcggcaagc gtctagtgcc aatcttggct gcctttgtca
    24721 tgttgctgat cagtgcagtg ctactgctcg tatggcctcc ggtttactct gctttagttt
    24781 cttttgctaa attcattttg agtttaggct ggggtggtgc aggtctttat ggcttcttca
    24841 accgtttact gatccctaca ggattgcatc aagcaatgaa ctccgtgttc tggtttgacg
    24901 tagcaggaat caatgatatt ggtaaatttt tggcttctga aggaacaaaa ggcgtaacag
    24961 gaatgtatca agctggattt ttcccaatca tgatgttcgg gcttccagca ggagcatatg
    25021 ctatttacag aaatgcacgt ccagaaaaca aagcaaaaac agcttccttg atgttagcag
    25081 cagcttttgc ttcattcttt actggtgtaa cagaaccact tgaattctca tttatgttcg
    25141 ttgcttggcc attatatgtc ttgcatgcaa ttttcactgg gatttcttta gcagtgagtg
    25201 cattcttcca ttggacagca ggatttgcct ttagtgccgg atttgtcgat tacttcctat
    25261 ctttgaaaaa tccgattgcg aatcagccat tgatgttgat cgtccaagga ttagtaacag
    25321 cagtgattta ctactttggc tttaactttg ctatcaaaaa attccatctg atgactcctg
    25381 gccgcgagga agcagatctt tcagatgacg atactgcaac gactaattca gacaataaat
    25441 atgcggcaca agcaagcaaa atctacgctg cattaggcgg agccgacaat gtaacttcga
    25501 tcgataactg tactacacgc cttcgtttgc aagtcaaaga tacaggcctg atcgatcaaa
    25561 ataaaatcaa agccactggc gtacctggta tgaagatcat cgacggaaaa aatgcgcaag
    25621 tcatcgtagg tacagaagta caattcgttg cagatgagat ggcaaaacta catggtggtg
    25681 ctgcaacacg acctgcacaa acaaacaccg tggttaaaac agaaacattt gccggtggaa
    25741 cgatcgaaca agatatttac gctatcgcaa atgggaaact gattccaatc actgaagttt
    25801 cagatgatgt attcgcggaa aaaatgatgg gtgacggcta tgctgtatta ccagaaaatg
    25861 gagaaatctt ctctccaatc gctggtacga tcacaaacat tttcccaacg aaacatgctg
    25921 tcggaatcca aacagatgct ggtatcgaag tcttgcttca tatgggcatc aacactgttg
    25981 acttaaaagg cgaaccattc actttatatg taaaagaagg tcaaaaagtc gcaagaggtc
    26041 aattgattgc tttagttgat cttgccgcta ttcaatctgc aggcaaaaac acggatatgg
    26101 tcgttgtatt cacaaatggc gacaaagtcc aaagcttaga aatcgaaccc gcacgtgatg
    26161 taaaagcaaa tgataaaatc ggctctgttt ccaacaaagc ctgagccatc gtcttcccca
    26221 gactttttta tcgataaaca aacaggagct gagacaaaaa tcttgtctca gctccttttt
    26281 ccgaataaaa acggcgttct cctgccaact ccgcggtaat aagtccagcc aaacgtaaca
    26341 tatgaagagc tattttttat ttgactcttc ttattacttg atgcagaaca gcttttttac
    26401 aacctctgat aaacggtgtt caaaaaccag tttatcggta tgaagtcaaa tttatgcaaa
    26461 aatgtggaaa gccattttca caaaattttt cttcatactc aaaactaatc ggctttttca
    26521 caacctcttt atacattatt cctgatccaa tatttctttt aatgctgctt caagagaagt
    26581 taatggtttt cccaatactt tttctaaatc atcagaggaa atagctaact gatccttttt
    26641 aatatcatgt tggatcgcta aaaacatgtc agctgcttct tgagggaatc cggcttcttt
    26701 tagattttcg acaaacgctg catcacttgc ttcaataatt tctaactttt tacctgtcgc
    26761 acgctctaat gccttggcta attcttcata gtcagttaga ttgccggata attctaagat
    26821 ttctggagag tcactttcta ctagtacttt tgctgcagct tctgcatatt cacgtttcaa
    26881 tgcccaacct gtttttccat ttcctgcagc ataaacgaat tgaccagttt ttaaagcctg
    26941 cccaatgata ggtaattcat tctctacata ccaattattc cgtaaaaatg tatgggaaat
    27001 acctgaatcc ttaatcattt tttcagtgaa ttggtgatct ggcgctaata cactgatcga
    27061 gtgatcggca tctgcaaaac ttgtataagc gatataagat accccagctg cttgtgctgc
    27121 cttcactaca ttcttatgtt cggcttgacg atttcctgga acgccagaga caaaaagcaa
    27181 tcgatctatt cctttcaacg cttctttcat tgagtctaaa tcatcataat caccaatacg
    27241 tatattaaat ccagcttctt tcagtgcttc tcctttttct tcactacgta ctagtacata
    27301 gatttcattt tgtggaacca tcttttttaa gaattccagc gcataaccac caaaacctcc
    27361 agttgctcct gttactaaat aatgcatatt tgccactccc tattcttttt atttgttaag
    27421 atttacagct gtacatttat aaattacacc tttatcataa agagtgtggt aagataagtc
    27481 aagcaaaaaa aatgaaagga tgacaactga tgaagtattc tctgcaattt agtgatgcaa
    27541 tccatgtact tgcctatatt gaaatgtttc aagatacccg atcactttct agtgagatga
    27601 ttgccagtag tatcgaaaca aatgcagcaa atgtacgtaa aatcatgagt cagctcaaaa
    27661 aatccggttt gatctttaca aaaaccggaa aaccgcagcc ttcccttgcc aaaagtccag
    27721 atcagatttc tttactagat gtctatcgca gcatcgaagg taatacacag cttatccaag
    27781 tcgatccaaa aacaaatcca aattgcgtcg ttggtgcgaa tatccaagaa gctttagaag
    27841 aaagctacgc tcggctgcag aaaaaggcag aagaagaaat ggctaagatc acacttgata
    27901 gtctgataca taaaatagcc gttttggaaa aagaaaaaag accagaaaac acaggcctcg
    27961 tagagagatt tttataattc tctctactgt gcctgcattt ttctgatcgg aatcgtctac
    28021 ataaattctt ttattctccg ggggacaaaa gccttgtcat gccccttttt cctctctaat
    28081 gctcaggtca aagcgctgaa gtcatgacgt cttgataaac ggtgttcaag tagctgaccc
    28141 tcggcattag ttctgaaaaa tgcaaaatat ggaaagctat ttcccatttt tcctctctaa
    28201 tgctcaggtc aaagcgctac tttcacgacc tctatattat catggtttat tgactaccgg
    28261 tatccagatt tctcccatat atttcccgtt ggtaactccc atttcgacta cagcatttgg
    28321 acccccaaca taggcgtaat cttctacttc tcttaaaaca tgcccaaagg ccgtattcgt
    28381 caaatcattt cttaattctt caggcgattc tgctaaagcg attacaacaa gatacgttcc
    28441 ttttgggaat gggatgattc gggtagtgtt tggaacatcc tgactcgttt gaacaccgac
    28501 atagtacatc attttcccgt tatagggttc gttgactgta aagaaataat gattttcagc
    28561 agcttgtttc aatgcatcta ttgttccatt accgataact ttttgctcaa atgcttgttt
    28621 ctctgcagcc aaaccgacaa aatccgtatt gctactcttc aattccacac caatacccgt
    28681 caccgtaaaa tctggttttt cttcgatcct atactttgcc atttctccca ccctttctat
    28741 tatattaagt tcatcttaca taaaattaaa gggaaatgac acgattacgc tcttttttct
    28801 agggatattt ttatttttta actatttttt tgctttcact gcaatccaaa tctcactgta
    28861 aacattactg aaatcctctc cccattttgt aaaagagatc tcgggagctt cgactaactc
    28921 ataatctgaa gaaggaagcc attctgcata gattttcccc caagtatctt gtaaagtttg
    28981 gggaaatggc cctttattag gaaaaatggc ccaagtgtgt tcttctatag caagcatttc
    29041 taaatcagcg tacggatttt cttttgtcgt agcaaagcca atcatgtgca tcaaactgcc
    29101 cttttcttcc aagcgggctc catcaaatcc ataagaaacg ttgattactt gatttggata
    29161 taaatctcct aacttatgca tctcttccct ttgagtctcc gtgatcgtct gcgctaactt
    29221 ttgtatttct tgattgactc cttcaaattt gacaggaact tttttcgcca cacctgccac
    29281 ataaaactgt tctttctttt cgatacgaaa ttccatcgat tttcctcctc tgacatcaat
    29341 aaaaaatgtc aagcgtggaa aagatttttg tatgccgtat ctgctgattt ctgaaggcag
    29401 atatcctgac caatcacgaa aagctcttga aaaaccttcg actgtttcat atccatatct
    29461 aaacgccgtt tctgtcactc ttgatccatc taggagatcg cgattagcca aagccaatct
    29521 acggtttttg atatagtcca ttagcgacat ccctgcaata aacgtaaaga tgcgttttaa
    29581 ttgatattca gagattcctg tattttcggc tattatttta taggaaagtc cttctgtcaa
    29641 atgttcttcg atgtaatcga tcaattggtt cagatcttta agcatattta tcttcctcct
    29701 tgcttgcctt tttatcatat tatcaaagaa agtcaattac ttgatgaatc aggaaccaat
    29761 ttagtcagat gtagaaaatg ttgtatggat ctaaagaaaa ttctcaagca gcgtttcgta
    29821 acggataatt ggtattatct gcctatctat gttttacctt tagaaaaata aattttgggg
    29881 aagacacatt ctaagtaaac aaagcggtaa aaatatcttt tattccctta gttaatccgc
    29941 ttactaccta ctgtacaaag caaattgttg ttcaaactgc tgatcagaac gctgcttgga
    30001 tttttctttg cgatacatca tacctctttt atcaattgga acaattattt atttctaaca
    30061 aaatcacttc atttttgtca gataaataaa aaacaaaaaa agttccatct ttaaaacacg
    30121 ctattctttt actttttctc acattctcta agaaaagaaa aggagaatga atttttgaca
    30181 attcgagcaa aagtgattat caaatatgta aaatcagcta cttttttctt aaacgtttct
    30241 ttatactaaa tataaataag tgtttcatga aaggagaact agatgaataa ttggcttgag
    30301 tattttcctg aaaacgtttt ggaacgcggt tacagctatc atctgcacgg ttttgtccgt
    30361 catttgaact atacaagcaa gtacttgtcc gcaacagttt ctggcacgga agattataag
    30421 gtagtgatca cttgggatga aaagaccaac atgacttgtg attgtctata tgccatagaa
    30481 ggaaaaaaat gcaaacacat ggctgctgtc ttattcgctt acgaagaacg tccaataaaa
    30541 aaatccaact actcactttc cgaactctcg tcccttgttt cctccgctag ttcctctctt
    30601 gtacgggagc ttttaacaga gattcttata gaacatccgc aatttatcga acgcttcaaa
    30661 gttaagatgc catttcatgc aataaattat tcagataaat taacaactat catccataaa
    30721 tacgatcata ttatcaagaa aaacaaaaac aggaaaacag cgaagttcat catggagatg
    30781 cgaaagttca tccaagaagc agttgaatcg ctcattcagc aaaatgccta tcttcccgca
    30841 ttcgaattga tcaatgaagt gattgctact ctcgaaacat tctattggga gcctgaggat
    30901 gaacgaaccc ttcttctgat agaggactgt tattatttat ggaaagaatt attagcagaa
    30961 gcacctcatg cagaaaaaag gcaaatgttc tcttggttcg tctgccaagt cgatcatact
    31021 gacgcctctt attcaaaacg ctactccata aaaatcctga aagaagactt tcgggaaaaa
    31081 gaattttcta accaaaagaa aaaaatagat aaaaaaacta aagaaacggt aaagaaagac
    31141 gatttcaatg aaaaatagca agtaaacgat aacaaccatc ctaaatccac aagaaataca
    31201 gcagaccttg ctttaaaaac acgaatcatg caaaggatga agtaaagaag acttaacgcg
    31261 tacccaatat tagtcatttg actattaggt acgcgttttg ttttataagt catgacaaat
    31321 ttccttcgtt gctgggtaga ttttccggca ttgttgatag attttatgat acttttctac
    31381 attttgtaag agaggttcca ctgcatctgt atattggata aaattctgac aacaactttc
    31441 aggtgtgtcg aaccactcta atctttaaag ataaatatta aaataacgat taataatatt
    31501 aacagtcttt atttttcacc accaaaaacc tatctttgca tatactaaat agacgtatat
    31561 acacagatag gtttgtcttg tgaaaaataa tgattatcct taccaaaaaa tgtttgtttt
    31621 tctacatgtt cgtacttatc aaaaaatatg atccttattg aaattactgg ctatttatat
    31681 tccaacgact tcttgtgtta atttttgtta tctcatagta aattatgttt cattttcact
    31741 taatttagtt gtcacgtgtt cgtcaattcg taaaaacggt aaatatataa acacacttgc
    31801 ggctataatt aatatttgta aaacagctgc tcgccagtct ccggctgttg ctaaaaatcc
    31861 cgatataact ggtggcgtag tccaaggaat ttgcactact acatgattaa ttaatcccac
    31921 agcagttgca aaatatgcta ctgtcaaaga aaaaatcggt gcaagcacaa agggaataat
    31981 cagactgata ttaaatacaa taggcaatcc aaaaataata ggttcgctta tattgaatat
    32041 agcgggagtt accgataatt tagcaaagtc cttataatct tttctacgac tgaagataaa
    32101 tattgcaatc aataaagcaa tcgtatttcc cgatccaccc attaccgcaa aggtatcttt
    32161 aaaggccata ttgataatat gtggaatttg ctctttgttt gcataagctg ccatattttc
    32221 ttgcatattc tgaatcaaaa acggatctaa caatgaacct gatattactg cttgatggat
    32281 accaaatcca aaaaatagat tagcaataga tgtaattaac aaaaatcctg gtaaaccagt
    32341 tgtaatttta cttaatggct ctgtaactat cgtggtaatt agcttattta tatccatatt
    32401 aaacaattga atgattataa atgacaataa agcaaacgag gtaatcgtaa taatgattgg
    32461 gattaataca ttaaaagact ttaaaacagc gggaggaata tttccggata gctgaatctg
    32521 tagtttctta ttattagaaa gtttaataaa taattctgta gccgctaatc caacaaatat
    32581 acctacaaac attcctgaag ctcctgtata ggatataggt atcacacccg tcacttcaac
    32641 agctttatcc gcattttcag gagtaaacat catctgcata ggtgttaata caacaaatgt
    32701 cgaaatagca actaataccg ctgcaatatg atttgtataa tttctgcttg cacacatatg
    32761 ataagatact gctgctacaa ctaataatgt agttatgctt aatgtaccat tggcaattga
    32821 agtaccaatt tgttgtaacg tggtcaatgt actagtatcg acaatttccc ccatgaatcc
    32881 tgttggttct aagatcactg aattaataag tgtcacaaat cctgctaaca tcataaatgg
    32941 cataaatgta gcaaacgcat ctcgcaaagt tcttaaatgg atctgattcc ccaacttagt
    33001 cgcaactata ccaaattttt caaatacaat gtccttcctg ctacttttgc ccatattttt
    33061 tctcctttat gcttattgta atcgattact ttttccaccc aaataataca tcacttatca
    33121 aaaaaagta
//
LOCUS       DACECZ010000017        31714 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.017, whole genome shotgun sequence.
ACCESSION   DACECZ010000017 DACECZ010000000
VERSION     DACECZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.017"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(106..573)
                     /locus_tag="IWO35_000803"
     CDS             complement(106..573)
                     /locus_tag="IWO35_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ0315879.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(693..2231)
                     /locus_tag="IWO35_000804"
     CDS             complement(693..2231)
                     /locus_tag="IWO35_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ0315880.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            2311..2997
                     /locus_tag="IWO35_000805"
     CDS             2311..2997
                     /locus_tag="IWO35_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ0315881.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(3198..3725)
                     /gene="msrA"
                     /locus_tag="IWO35_000806"
     CDS             complement(3198..3725)
                     /gene="msrA"
                     /locus_tag="IWO35_000806"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0315882.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(3809..4486)
                     /gene="pgmB"
                     /locus_tag="IWO35_000807"
     CDS             complement(3809..4486)
                     /gene="pgmB"
                     /locus_tag="IWO35_000807"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0315883.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(4483..5613)
                     /locus_tag="IWO35_000808"
     CDS             complement(4483..5613)
                     /locus_tag="IWO35_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0315884.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(5613..7796)
                     /locus_tag="IWO35_000809"
     CDS             complement(5613..7796)
                     /locus_tag="IWO35_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0315885.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(7793..8818)
                     /locus_tag="IWO35_000810"
     CDS             complement(7793..8818)
                     /locus_tag="IWO35_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0315886.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(8863..9690)
                     /locus_tag="IWO35_000811"
     CDS             complement(8863..9690)
                     /locus_tag="IWO35_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0315887.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(9687..10754)
                     /locus_tag="IWO35_000812"
     CDS             complement(9687..10754)
                     /locus_tag="IWO35_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ0315888.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(10780..11616)
                     /locus_tag="IWO35_000813"
     CDS             complement(10780..11616)
                     /locus_tag="IWO35_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0315889.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(11613..12482)
                     /locus_tag="IWO35_000814"
     CDS             complement(11613..12482)
                     /locus_tag="IWO35_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0315890.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(12552..13835)
                     /locus_tag="IWO35_000815"
     CDS             complement(12552..13835)
                     /locus_tag="IWO35_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0315891.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(13849..15534)
                     /locus_tag="IWO35_000816"
     CDS             complement(13849..15534)
                     /locus_tag="IWO35_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ0315892.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            15809..16834
                     /locus_tag="IWO35_000817"
     CDS             15809..16834
                     /locus_tag="IWO35_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0315893.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(17429..21082)
                     /gene="rpoC"
                     /locus_tag="IWO35_000818"
     CDS             complement(17429..21082)
                     /gene="rpoC"
                     /locus_tag="IWO35_000818"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ0315894.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(21161..24778)
                     /gene="rpoB"
                     /locus_tag="IWO35_000819"
     CDS             complement(21161..24778)
                     /gene="rpoB"
                     /locus_tag="IWO35_000819"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0315895.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            25506..26474
                     /locus_tag="IWO35_000820"
     CDS             25506..26474
                     /locus_tag="IWO35_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0315896.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            26492..27397
                     /locus_tag="IWO35_000821"
     CDS             26492..27397
                     /locus_tag="IWO35_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0315897.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            27394..28200
                     /locus_tag="IWO35_000822"
     CDS             27394..28200
                     /locus_tag="IWO35_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0315898.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            28357..29325
                     /locus_tag="IWO35_000823"
     CDS             28357..29325
                     /locus_tag="IWO35_000823"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ0315899.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(29642..30166)
                     /locus_tag="IWO35_000824"
     CDS             complement(29642..30166)
                     /locus_tag="IWO35_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ0315900.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            30453..>31714
                     /gene="scm"
                     /locus_tag="IWO35_000825"
     CDS             30453..>31714
                     /gene="scm"
                     /locus_tag="IWO35_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ0315901.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTS"
BASE COUNT         8914 a         6858 c         5972 g         9970 t
ORIGIN      
        1 aaaaacggca aaatatgagg aactattttg acgtttgttt ctctaatact caggtcaaaa
       61 cgctgatttc acaacctctt tttcggtcaa gttcaacaac agtttttacg cgtcgatctc
      121 tggcgctttg cccaaatgag cttggatcat ccagatacgt ttttctgtgg ctgttttgaa
      181 ttcgataaag atatcttgtg ttggatcgtc gccttcttct ccagaaactt cgattccttt
      241 ttggtaaaga tctgctagat aacgatagcc tgcaacaagt gtttccaaac gttctggaat
      301 agtacgatcc cagcggccaa tttcatcagg gatttttgtg ttatctgcaa attcacgcaa
      361 tgtagaataa ggtgaaccgt ctaatgtgat caagcgttcg gatacttcat ctaactgagc
      421 gttcaaatca tccatgaact catccatcat tgggtgcaat gtaaggaaac ctggtcctct
      481 catgtaccaa tgtgtttggt gaacaacaac agacatttgg cttagatctg caactaattg
      541 atttaatact tctttggttt gttggaattt catctttctt gcctcctaac attttcttac
      601 acttaaaata atagtagact ttcaatgaaa agtacaacaa aatcatttac ctgagaacga
      661 ttttcagttc gcttttttat ccgtaaagcc aatcattttt cagaatcagc taattcttct
      721 cgcagttgag caaattgctg ataagtctgt gctaaatgct tttgtgtcga aacaaataat
      781 ggatagattt tctgatattt tttatgatca gtttcttgta ctccaacttg ctctttcaat
      841 tctatcaatt cctgtgcttc actgatactt tggatctttc cgatgcttct ccagcctaaa
      901 atcacagctc ctaatgcgga tgcttctgtg acagtaggga aaacaagcgg gtaacccagt
      961 acatcagcca tcatctgacg aaaaatcgga gattgagcaa acccgcctgt agcacggatc
     1021 tctgtgagct gattttcctg gcaaactcct tttaaaatcc gataaaggtt gagacagatc
     1081 ccttctagaa tcgccttcat catttctttt tctgtatgtt gccgctgtaa accgataaaa
     1141 ctgccaaacg catctgcgtc ccaaagcggt gctcgctcac caagtaaata gggatgaaaa
     1201 atcagaccat tggcacccgc tggcacaaaa gcgatagccg aaagcagtgc atcatatgga
     1261 ttttctcctt tttctttagc agattgaacg acttcctgta aaaatgtatg ccctgcccaa
     1321 tcaaagatgc cagcaccatt gctagtcgct cctcccacta cccaatgttt ctgatccaga
     1381 acataacaaa acgtctccat ttctggatgg aactgaggct gagtagtaat aaaacgaagt
     1441 gctccgctcg ttcctacggt caaagcggcg atcgtatcac caaaagcacc taaccctaga
     1501 ttagaaagcg gcccgtctgc ccctcccaaa acaaatgata tatcatttgg caaacctagt
     1561 tctgctgctg tttttgatgg tagacctgtt acttgatagg tggaaggaaa gacttgcggc
     1621 aattgctcta ttcgaatgtt caagtagtca agcacttctt ctgcccattg gaattcatgg
     1681 atatcaaaat atccagttcc tgaagctgtt ccgtagtcgc tgatcagttc gccaaacaat
     1741 ctataaaaga tatagtcttt gataccgaca aaataagcag tttttttgaa taattcaggt
     1801 tggctgtttt gcagccactt gattttgttc aatggtgtca ttggatgaat cggcgtccca
     1861 gtctgccgat aaaattcttt gccaagcacg gtatatttca gtttttctgc ttcgtccgct
     1921 gctcggttat ctgcccaagt gatcattcga gataacggtg ttttctcttc atccaacaat
     1981 atcaggccat gcatcgcaga ggagaaggag acaaattgaa tgattgtgtc tgaaggcaac
     2041 tgtttaatta cttcttttat aacggtgcag acaccttgaa agatcaattc ctgatcttgt
     2101 tctgccatcc cttgtgtttc ttggatcaaa gggtagcgaa aataagcgga tgctgcttct
     2161 tgggcttcat ctgtgaaagc aactgctttg gtttgtgtgg ttccgatatc taatccgatt
     2221 gaaacgttca ttttttcacc tcaaatttta tgtatgtaca aaaatttagt ttaagcttgc
     2281 gtatcttggt tttgaaaagg gggagatggc gtgttacttt attttatcat tggttgctgt
     2341 atcggatcat ttttatgttt aaccgcccag cggattccgt taggtcactc tattatttat
     2401 cctagatctc attgtgtcaa atgctgtcgt tcgctttctt ggtatgaatt gattccgatt
     2461 ctctcgatca tcgtccaacg atttcgctgc cgctattgca ggtgtcggtt gcccttctac
     2521 tatttgttgg cagaagcctt atgtggtggt ctttttgcct ggttctttac tttttcttcc
     2581 gcaagaaatt tttcgacgtt catatggatg ctttcggctt tgctattttc gttgatggat
     2641 ttattttact tcatggtcca ttctcacacg ctattctgtt cttgggcaat tttatggatt
     2701 ttttggttgc aggccggcgt cttccaatgg caaagtgtgc tgttaacgtt tatcgtaggc
     2761 ggtgtgtgcc ttcgctatgg tcaatcctat ctcggagctg gcgatactct gttgatgctt
     2821 tcttggagtg gtggcttgtc tctagaagag ttgcttcaga tcttgtttct tgctagtctg
     2881 tttggaatat ccgttttttc tgtttacttt atctgtcata aaaaaaagtt ggaaaagctg
     2941 cctttcattc cttttttgag cagtgcgttg ctgattgtcc ttcatcttag gtattgaaaa
     3001 aaggttgtga caaacgactt gtcacaacct ctgatatata gtgttctctt accaaatcct
     3061 tgtgacttgg agctaggcga caggttcaca acctctccac taaatggtgt tcaatcagct
     3121 gaccctcagt attagctcaa aatgctggtt ttacaacctc tgatatacgg tgttccataa
     3181 gtagcttttt cggcttctta tttcttgacg ccccaatgtc gacgaaggaa acctgcacgt
     3241 ccagaacctt ctcgataaag attgtaatgg agcttgtttt tcttgtaata atcctgatgg
     3301 taatcttctg caggataaaa ttcgcttctt ggttcgattt ttgtcacgat tggcttatcg
     3361 aatcgcccgc tgtcttgcaa tgcttgtttt gaacgctctg caatatttcg ttcttcttca
     3421 tcattatagt agatcactgg acgataagaa tcaccgcgat cagcgaactg tcctaatgcg
     3481 tcagtcggat cggtttgctg ccaatagatt tccaccaact gatcataaga aataacttct
     3541 ggatcatatt cgatctctac tgcttctgta tgccctgttg tgcctgttgt tacttgttca
     3601 taagttggat ttggtacatg cccgccagta taaccggatg tcacagaaat gatccctggc
     3661 tgctcttcaa atggtttgac catacaccaa aagcatccgc ctgcgaaaac tgcttttttt
     3721 gtcatataaa atcccccttt tttctcatca taccttgatt tccaaaagga ggataacgtt
     3781 ttgcttgtga cagaaaaaac ggaaactatt atatacgtga tacggcttct gataaatgcc
     3841 aaaacgaacg tacttgatca aaaacggtta aatcaggaat cacatattca gcagaaggaa
     3901 gatttatgtt ttcacctatt cccaatacat gcatgttacc agccaaagct gcttgacaac
     3961 cagcttctga atcttcaatg accaagcatg catttggcgg tacattcaat tgctgtgctc
     4021 ctttgagaaa aacttctgga tcaggttttg cttttgatac ttgcgtgccg tcaataagga
     4081 catcaaaata acttgtcaaa ttcgtttttt ctaaaatgag gcgtgcattt ttgcttgctg
     4141 atcccaatcc gatcttgatg tttgtttttt tcaagtactc aagaacagct attgcaccag
     4201 gcaaaacgga tgtttcatcc attttttgaa tcgcttgaag atatagattg tttttttgct
     4261 cagcaaaggc tgatttctct ttttctgaga atttctgttc catttttcca aatgacagca
     4321 atcgctctag tgactcggtt ctggatattc ctttcagttg ctcgttttct ttctcagtga
     4381 atggaatgga cagctggtta gctaaaacca gccacgcttc gtaatgataa cgagctgtgt
     4441 ctacaagaac gccatctaaa tcgaatagtg ctgctttgat tttcatcgat tacctccttg
     4501 aaaatgaacg acttgaagta aagatgcgat gtttttagct gtgcgatgaa gattatcagt
     4561 ggcttgctgt aaagcttctt gtaaaggcac agctgcagga aggatcggaa aaacggccga
     4621 aatgccaaca tctgctgctt tttcgcttcc tttaccaaca cttccgcaaa tagctatcac
     4681 aggaacgccc tctggtactc ttctagcaat cccaactggc gcttttcctt ttagactttg
     4741 tccatctaga cgtccttcgc ctacgattac gaggtctgct tgctgtaccc gtttatcgaa
     4801 ctccagtaaa tccaatacgt agtcgatgcc agagactatt ttccctgaag caaatgctaa
     4861 cagtcctgca cccattccac caccagcact actgcccgat gcctcgatta cttgaggggc
     4921 aaatctcata taaaaatcat gcatggcttg gtcagccact gctaattcat tttctttcaa
     4981 gcctttttgt ggtccgaaaa caaaagaagc gccttctttt ccgcataaaa gattagtaac
     5041 atcagtaatg acagtgattt tggctttaat cagctcaata ggcacgttcg ttttatcaac
     5101 tgtagcaatt ttacctagat tttctcccag tgcttctact ttttgattat cttgatcgta
     5161 aaaagcataa cctagacctt ctgccatgcc gatgccgcca tcgttggtac tacttccgcc
     5221 aacgccgatg atgatgtgtc gaacaccttg ttgcaacaat tgtcgtatca gttctcctac
     5281 tcctcgcgtg gataacctaa gtggctgttg ttggtttagt gggacatctt tcaatccgac
     5341 gatttctgcc atttcaaaaa tcgcagtttg accgtctgaa gcgtactgga tttctttgtt
     5401 tttgccaaat gcattagtaa cgactgctgt ttgccgcttc aaccttaaac tgtctgctaa
     5461 tgatgaaacc gttccttctc ctccatcgcc tataggtagc agatcatatt cagcctgaac
     5521 aaaaacttct tgaaaacctg attgaatggc ttgtgctgcc tctaaggctg tcagactttc
     5581 tttgaaagaa tctggcgcaa tcagaatacg cattattttc ccctccgttc caagatcagt
     5641 tctttttcca gctggtattg gtctccattg acaaaaatag gtaatgtttc tttcgtctct
     5701 ttttttatta ctatttgttt ttttgtcaaa acaaatagca acctgtgatt tttccaaata
     5761 aaaggaaaag aaattttttc ccatgctgaa ggaagtcttg gagaaataga aagagttcca
     5821 ttcgttactg taatacctga aaaaccaaat ataactgcta gccatagtgc tcctaatgaa
     5881 gcagcatgaa ttccgtcatc tgatgaatgc ggatctggtc ctaaatcaat caggcacgct
     5941 tcttggaata aatgatacgc ccattccatt tcttggatac ggcaagcctc tatggcgtga
     6001 atcgctttac tcaacgatga gtcgtggatt gttttgtttt catagtaatg caaattctta
     6061 gaaacgattg gttgagaaaa atactcaggt aataagtata acagcatgac aagatctgct
     6121 tgcttgctga cttggagttc atttacttcg ctacgggcat aatcaagtaa aatagactgg
     6181 ctgccaggac gttctttgta agtggtcaaa tcaataactg gcttactgag atacgtgtca
     6241 tcttgcggaa tcagctgttc tttgttaggc actggcaaat ataacttatc taaaaaattt
     6301 tgacattctt tcaaaaaaag cggatcgcca gcttgatatt taacagctaa ggccacatta
     6361 taatgcgcca gatagttcgt atatgcatta ttgtcgatat attctgtgta ttcatctgga
     6421 ccaatgactc tgtgtatctc taaatgatca tcgactttta cagctctgct cagccaaaaa
     6481 gctgctgttt catgaatgag cgtttttccc tttgtttcta aaaacgattg atcctctgtt
     6541 gcttggacat actcttgaac ggcataagca aggtctgcac tgatatgatg ttctgcggca
     6601 gctgaagcaa cttcttgtct tgttccggtt ttgatattca atgcagcaaa tttgggtgtc
     6661 tcttcctgac cagtgtgtgc gctttcccat gggaaaagtg caccttgata gtgataatct
     6721 cttgcttttt gccgtgcgcc ttctaatcga tcataacggt acagcagcaa attctttgct
     6781 acttcaggaa actgatataa aaagaaaggc aggataaaaa tttccgtatc ccagaaaaca
     6841 tgccctttat aaccttcgcc agttagtccc ttcgccccta cactaacact tggatcattg
     6901 cggggagtca taatctgtaa atggtaagca gcgaagtcca tcgctaactg atcgaaagaa
     6961 gaatcgcctt ggatcgttat ccgatgctga tgccaaaaat ctgtccaata agacatcgac
     7021 tgctctaaaa tttctgtata actaacgctt ttctgggctt gggcaatact tttcttaaac
     7081 gggtctgttt gctgaagaga ggtagagagc acgatgatct tctccagcgt aaaggacgta
     7141 ttttttctga tcttttctga aaaggaagcg gtgatccggc gattttttgc ttggaataca
     7201 cctgctttat tgaatttagc tgcaatccca atatcaattt tactttctgt tgtctggtag
     7261 gatgcactga tcaaatcgtc accaaatact cgcagttctt tttcaaccaa ctgctgtgta
     7321 ccaaaattgg tttgctgtcc atcgataccc gttttcacat ctacttggca gtcttcggtc
     7381 aacggcttga tgaccacttt cagggctgcc tgatggagat tattttgatc agcaacacga
     7441 caagtaatga cttccacttt ttttccttga gcagtttcaa tgagcgcata gcgtttaagc
     7501 tctcccattt ctaaatctag tgcccgctcg tatgcaagaa ctttcatcgt ctcaagagaa
     7561 aaatgttcac cgtcgatttt tatttcccat ctgatgacat ctgggagatt cactaactcg
     7621 gctgattctg ctcctaaagg tcggttatat acaccagctg caaacattcc ccttacttga
     7681 ttccgatagt cttcctcctg gcttcctctg ataccaaaaa atccatttcc aatggtcatc
     7741 tgactcgcat acttattgat atactctaaa tcaaactgtt tttcttctac tttcataagg
     7801 taataacttc tcctgtttct gccgaacgat ataaattttc caccagattc tggatgacat
     7861 atccttgcca gccatctgca atcatctgag tctggtcacc gagcgcccga ttcacaaaat
     7921 gagccatact tagttcatgt gggtcattaa ctggcgtggt cttttgtaag gaaaccagct
     7981 ttccatctac atcttcgaat agttcgcctg gatacagatt agcacctgcc tgatcgccac
     8041 aaaaagccac attcagtttg tcttctctga tattcaaagc aaacgaagta ttcagttgga
     8101 tgatcttgcc gttttctaat tccagtgtgc caaaaattgc atcttctact gtgaatttgc
     8161 ttggatccca tttcccgaat ttccctgaat tttttttcgt accaattttt tggaagctgt
     8221 acgcccgaac gctttttact ttaggaaatc cagtaatata catcgctgta tcaagcatgt
     8281 gaatgccata atcgatcaaa ggcccgcctc cttgaagttc tttgtcgata aacgttcccc
     8341 agcctggaac accagaccga cgaagcgcat tcgcttctac gtaataaata tccccgagaa
     8401 catcaagatg attcttgatc attgttgctt cgtcagaaaa acgatgctgg aatccataag
     8461 ccaaaatctg cccgttttta tcggctgtct ctgccatctt tcttgcttga tcagctgtca
     8521 tagctggtgg tttctcgcac atcactgaag cattagcttc gagggcagcc aatacatgtt
     8581 cgaagtgaaa gcgattgggt gtacagacac tcacgatatc gggagaaatc tcattcagca
     8641 tttgtgtaac tgatgtatac gcatgagaaa atcctgtacg tcggcaaaag tcttctgctc
     8701 gctggctgac tggatcaacc acagcggcca attctaagac atctgatctt ttttgataat
     8761 aagcagcatg tactttttct gctacttgtc cagcgcctat gattgctact ttcttcattt
     8821 atttctccct cctttgctta tttacttatt gttattcttt ctctattctt tctctaaatc
     8881 atttaaatga cgcgctgttt ccagacagtt tttcaagtat tgcaatgatt cctgataaat
     8941 tttcacttca ttttctcctc gcaaacgaca ttcatatacc atataacctt catactcatt
     9001 ttgaagtagc gtttgaaaat ggcgagtaaa atcaattgcg ccagatcctg gttggtagcg
     9061 atgattatct gctaaatgga tatgacccac atactctttt tgcgtagtca atgcttttga
     9121 aatatcatct tcttcaatat tcatatgata aaaatcagcg atgatctgca catgtttcaa
     9181 ttgattagag tcaatgtaat gacgcgcatc cgctaacaaa ttgatcatat ggtcttggta
     9241 tcggttcaaa ggctccaaat aaataaccgt acctgtttct tcggctactt tatctaaata
     9301 acgaagtgat tcgctgactg ctttatagtc tccttctttg cttcggggtg aaaccatcgg
     9361 cggtaatcga taagtgaaca ttccccaagc tgctgggata acgattcctt tgcctccaac
     9421 ctcgcttagt gcacgcaaaa tctcagcaat ttgacgtaca ccattaagac gccgttcttc
     9481 gataaagtca ccgatccacc catcgtagcc gttacaagct gtcactactg ggatccctgt
     9541 ttctttgatt gcctgtttga ctgtgtcgat attttctatt aacaacttac cgtcgatttc
     9601 gtattcttca aatccacagt ctttgataaa tctaaatttt tctaaaatgt ctcttgggaa
     9661 aaatgcttga tcttgcgtac cgaatttcat tgttctcctc ctcggacttg aacgcccatt
     9721 ttgatacttt cttctgggtg ctggtctaca taacgcatgt aagcttctgc gctttctgca
     9781 aatggcacaa ctggatcaat gatttcttca caatccaagt aaccgttcat caataattcc
     9841 caacatgtgt cctcgattcg tttacggttc catcttgggt aatctgggtt tggttcacta
     9901 gatgctcgag caaagacgat ttttgcatta ttgaaatgtg cttcgcgacc aagattaaac
     9961 ccagctggaa aaggtttagc aaaggcaaca taagaaatga tcccgccata gccgatccct
    10021 cttaaagaag cttgtaatgc ggcagcactg ccactcgttt caataactga atctactcca
    10081 cgtttatctg tcaattcctt tagacgaaaa ccgacatctg ctttcatagg atctagcgtg
    10141 taatcggcac cgtatttttc tgctaattct ctacgctgcg ctatgggatc gatcccaacg
    10201 atggtagtag cacctgcttt tttagctaat tgaatcgcaa tttgcccgat agcgcctaaa
    10261 ccgaagattg ctacagcatc acctgctcga acatgtgctt cccggatacc acctaaagcg
    10321 aattgagcag gatcgtaaca tactgcattt ttccattgat ccaaccgatc aagtttacgt
    10381 agtttataat tatcaacacc gtttgtaatc agcgtttcac gaatgggtcc ataagtgcag
    10441 actaactctc ctttttgata ctctgtcacc aaactgcccg tttcttcgat cgtaccgaca
    10501 atcatatttc ctaattgaaa ttcgccaaat accacaccgc gttggcttcc ttcttctctt
    10561 ggcaagaata ggcgccattc ttctgaaaat tcttcatcga taaatggact taatcctcta
    10621 aagtcgacaa tttctgttcc tggttttgga gtagcgaaaa ttactcggat acgtacttcg
    10681 ttcttttcga tctcacggtc ttcgtaatct ttgaatccgg ctattcttgg ttctaatgca
    10741 actaattttt tcatcttttc tttcctcttt cttttctttt tatcctttta cgccgccttc
    10801 gactaaatca cctttaataa atcgttcaga taacgcatac ataatgatga ctggtaaagc
    10861 ggtcaccaat gaagcggcca tcattcgtcc ccaaatgtag tctggtgtac tgaataatgt
    10921 gttcaaaccg attggtaaag taaatttttc ggaggtcgac aggaaaatcg aagcaaataa
    10981 ataatcattc cacgaaacca taaagcaata aacgaagaca gaaatgattc cagaaatagc
    11041 caatggtata atgatccgaa taatgatctg gatccggcta agaccatcga tcattgctgc
    11101 ttcttcaata tcagtcggaa tcgtgtcaaa ataacttttt agcatgaata tcgcagtagg
    11161 aagtgtttga acgaccatcg tgataatcag tgctgtattg gtatcataca aacctagacc
    11221 ggaaatgatt ttaaataatg gaacaacgat caaaataccg gaaaacatat aaacggtgta
    11281 aaagctagca ttgattttcg agcgagcttt gaagcgtagt tttgacaaag cgtatgcccc
    11341 gctgatccca atcactactg cagttacgga ggctatcaag gagatcacta aactattttt
    11401 gaaatacatg atgaatggaa aaatctcagc attgaaaata tcctgataat gttcaaaagt
    11461 ccattctttc ggaaaaatag tttgtgtcgt tgcaattgct tcttgtgaat ccttgaatga
    11521 tgtcatcagc atgatcaaaa atggaaacaa attgacagct aatagcaaca aaatcgtcgc
    11581 gtaaaataag attttcctgc tctttttatt tttcatttca agtccacctt ctttcgtgag
    11641 agcaagacga ccgctgcaat aatgatgaac aatagaatcg atatagctgc tgcttttcca
    11701 agatcattga acgcaaaggc tgtctgatac aaataaacgc ctaagatatt gactttattc
    11761 gtcagtagat agacatctgt aaacatatag aacatccaga tagatcgtaa aacaatcaca
    11821 gttgctagta ctggtttgat tgccggcagt gtcacgatct tgaacttctg ccaagcattt
    11881 gcaccgtcca tttcagctgc ttcataaagc gttttgtcga ctgtttgcaa aatagccaaa
    11941 aatgaaataa acgcatacgg atagtatctc cagatagcaa acaaaacgac cataaagaag
    12001 ctagcaactg gctggtcgaa ccacagcggt gcttcgttga aaagctgcaa cctatctact
    12061 aacagatcat tgacgattcc ataactatta ttgaacatat atttccaagc aaaaatcaaa
    12121 gaaatagatg gcgtaacata acttaataaa atcagtgaac gcaccgtttt cctaaatctg
    12181 aacttgcggt taagcaaaat agctacggct aatcctaaag ctgtactgcc tgctacaact
    12241 aagaatgtgt aagcaactgt taaacctaaa gaacgataaa attcactatc acttaaaatt
    12301 tcaagataat tgcttaatcc gataaaaacg gcgtctgagt taggattcag cggcatatcg
    12361 aaaaaactga tctgaatatt ggaaagcatt ggaatcagca ctaaaacagc taataaaagc
    12421 atgcttggtg ctaaaagtcc catggctaat tttaagtcag aatgtttcct tggacgattc
    12481 attgaaacct ccccctgata atctgccaat agctatctac ttttttcgaa caccattggc
    12541 agaacttctt gttatttcgt atttttttgt gcgtcagcta aagcttgatc gacatcttcg
    12601 tttccgacag ttacttggtt cactgctttt ccgatcacac ctgagcttgt gatatctccc
    12661 attttcgtga aattcttttc cccgactagt ccaaagactt gtacttcatc aaaagcacta
    12721 gcaatctcag atggcagctc gccaaaagac ttgatgactt cattttcttt gtaaccatcc
    12781 agttcgacta cttgtttatt cactggttga gcaccaccag gagacatcaa gacccacttt
    12841 tccatgtttt tcggttgaga taagaaagct gtgaatgctt cagcagcctc tttttgtttc
    12901 tcgtctagac cagaagaaat cgtcaatcca gatactgtgc catagacagc ttgatctttt
    12961 tgtgttggga tagcaaaacc gatattttct gagttccccg cttcataaac agaaggcaaa
    13021 atgtaagttg aatagattgc cattggtgct gttccattca tgaaagcatc tttgatttct
    13081 gtcacatcat tagagcctgg catcgtgtat tgggataagt tttgataaaa cgacaatgct
    13141 tctctcatct tttccgtatc gatcgttacc tcgccttcat catccaaaac atttgctcca
    13201 tttgacaaag caaattgaga gaacgcttgc tcactcattg ttccttcagc agtaggcata
    13261 gcaattccat attgtttatt ctctttatct gtgaaatgtt gagcaatttt taaaatatca
    13321 tcccaatttt ctggttctga aaaaccagct tctgataatt tttctttgtc ataccaaata
    13381 ccttgaaccc agccgctaat tggtgcagca atatagcttt ttccatcttc tgaacgaaca
    13441 aggtttttgg ctccgtcata ataattatct tctccagctt cctcgataac gttttggact
    13501 gccgtctgat caatcagttc atctttatcc atcactttag caaaatcctg actgacttcc
    13561 attacttctg gtaatttacc ggaacgagct aacgtaacga tttttgtatt gaacgctgat
    13621 tcttctaccg gcacttgctt gattttgatg tctggatttt ctttttcaaa atcagcaacc
    13681 agttcattga tcacacttaa tcgttcctgt tcgacagaag agtgcatgaa ttgaatcgtt
    13741 gtctgttgtg attcgttaga gctttgactt tcaccacaag cagtaagaag aaatccagta
    13801 gctagcagac aggtagtaac aataattttt ttcatttttt tccacctctt attttattat
    13861 tttcagccac atatattcat acggctggaa tatcaacttt cgttcttgac tgcaatggat
    13921 aatttgtcca gaaaacaggt ctaggtaatc tccttttggt aaaagataac ctgctgattg
    13981 attggaaaaa ttatggataa ctaagacttt ctcttccttc gcttcgcgaa taaaactgat
    14041 aaaagaatct gctgagtcca gtaagtaaat caaactattc ggatggaagg ctttttcttt
    14101 ttttcgaata gatagcatct gggtcaattc accatatact gctgttctta atggatctgt
    14161 ctgcaactct ttctcaatat ctgctaacaa aaatttttgc cgattgattt ttcgattctc
    14221 tcctgttttt tctacgcctt tgtaatcatt tcgacttccc agcacacttt gaatataaat
    14281 tgccggcacg cctggtaacg ttaccaaaaa tgaatgagca gctaaaaagc gtttgcttct
    14341 ccattccgtc gtatctgttg gagaacttaa cgcatctaga tacgtagcgt ttatctcata
    14401 tggactggtc gttccatctg ggtttttctt gtagtttact cgggtaccct ctttcttcag
    14461 ctgttccact aattcaagga tcgttttttc cggcagaata cctcgtaccg gattcaatcc
    14521 gataccgtca tgggaggcta aaaaattaaa aaaagttgtt tttttcgatg cgaaatcgat
    14581 ggtttttaac cattctttca acttacttgt attacccgaa cgaatcgctt gaagggtcaa
    14641 aggtggtagc gaaaactgat agaccatctg tgcttcattc tcgccattcc caaaataact
    14701 gatattatct ttgtgtggca catttgtctc agtgatcaat accgttcctg gagctgcttc
    14761 atcagcgatt gcacggaata attggatgat tttatgcgtt tctggcaggt gtatacaaga
    14821 agttcctagt tctttccata aaaatcccac agcgtccaag cgaataaatg aagcgccttt
    14881 ttctaagtaa aacaaaagaa tatcgatcat tttcaataaa acttctggat ttttaaaatt
    14941 caagtctact tgatcttcgc taaaagtcgt ccatacgtgt ttttctttcc cgtttttgtc
    15001 aatgaacgtt gtaagtaacg ggctggtcct tggtctagtc acgctgcttg tatctgtatc
    15061 cagtggcaaa tcaataaaaa aatctttgta cgtatcattc aagtttaaat attcctgaaa
    15121 ccaagtactt tcggcagaca tatgattgca gacaaagtcg aacattaacc gtgtttcgct
    15181 tctcatttct tctatatctt cccatgtgcc caaacggtcg ttgattgcct gataatttac
    15241 aacggagaaa ccatcatcag atgtatatgg gaaaaatggc aagaaatgta caactctaaa
    15301 tgtatctgcc aaatactttt gataaaattt cttaaatgta cgcaaagttg gttgctgctc
    15361 ttcaaaaaat tgatccccat aagtaatcaa gaaaatatct tcttcgtccc aatgcagttt
    15421 gcctgcctgg cctttcttct gctgataaac actgactttt tgttcaatca tagaggcggt
    15481 tttctcagac aaatctgagt cgcgataaat cgcttgtaaa agttttgtat acatcggatt
    15541 cccctttctt ttttgggagc gttcccaacg atttgatcat attatggaaa acgcttactg
    15601 tcaatagcat ttttttaaat aattttgata tacacaaaaa tacaaataag aaagcttatg
    15661 tatcaataat taatagcttc ttaaaaaaat atggtctctc atgatcatgc aagctattcc
    15721 taaattttca aaatcataga caaaaaagaa tgtctattgt actatggaat tattctaaaa
    15781 cgctcccaaa aaggaggtgc atataaaatt gaaacctact atttatgaca ttgcgaaaaa
    15841 agctaatgtc tcaaaatcaa ctgtctccag agtattaaat aaccagccaa atatctctga
    15901 taaagcaaaa gcaagtgtac ttcaagcaat caaagaattg aattatgaac cgagcaaaat
    15961 tgcaagagga ctgtcaggaa atggttttga cgcgattcta gcattatctc atcgtacttc
    16021 tcatacgacc tatggcaatc cttttttttc agatgttatt caatccattt ctgccgtttc
    16081 tgaacaaaat aacttcgact tgattctaca aacggcaaaa acaccagaag aagagctaga
    16141 aaaatgcgcg aaaaagatcc aagaaaaaat gatccgagga attattattt taagctctac
    16201 gatggatgaa acacttcttt ttgaattgga tcaatacaat attcctattg tggtaatcgg
    16261 aaaagtcgga aacgactaca cgaacatcta ctcagtggac acggataatt tccaggatag
    16321 ttacgatttg gtggaaacac tgatcaagaa tggacaccgt catatcggct gtctccatgc
    16381 tccgacagaa gtttatgtat ccaatgatcg tctcaatggt taccgtcaat gcttatttga
    16441 tcaccaccta gatatccgta atgaatggat tatagacggc ggctttcgct tagaagatag
    16501 catggcagca atgaatgatt taatgaagct acgagaaaga ccaactgcga ttttttctac
    16561 cgatgcatta aaatcattga gtatttacaa agtggcagaa caatctggaa agcagatacc
    16621 tgatgatctt tcgatcgcag gattcagtga tcccttattg tcctccctat tttcgccaca
    16681 gttgactcgg atagacattc cgacaaatca gttaggaatc aaagccacag aacttttatt
    16741 ccaactaatc caccaccaat accctgaaga aaaaagaatc attctttcaa ccgagattac
    16801 tctcacagat tcgatccaac aacaagaaat gtagaacaga attataaaag aacagaaaca
    16861 ctgaggataa gtcagttact ctgttctttt ttcaatcact catccctcgt taccccctcc
    16921 cggtatttta gcgaattttt cttattttcc atctgtttta gatagctgtg gataactata
    16981 atgtttgtgt taaactattt tttccacagg gaagaaaaaa ctatttttaa ccacgttgtg
    17041 tataagtttt tatagttcgt ttttttaaac acatttaccc ttatttcaat ccatgttatt
    17101 tggggatact tatctctaaa acataaatag aataacagac atgtctactg gttttgtttt
    17161 attcgaggaa ttattcaata atagctagca aaatattaaa aataaccgct ctatcttccc
    17221 atcaatttat tttgcattca aaaaatgatc tatctttact tttgttgatt gacttctctt
    17281 ggaagatgat cgttccgata ccttaattgg atacgaaccg atgttgttaa gatcctttcc
    17341 cacttttctt ctagttagac aaattatttt agacaaacca aaaaagaggt tgtgacaata
    17401 atttgtcaca acctcttttt taaaatatct attttgtttg attgttcatt gcgtcttctg
    17461 cagccatttg cgcttcaata tctgaaatac tgtagacatt ttcgcttgct acaccgactt
    17521 cttttggttc catgtcacga taacgttgca taccagtacc agctgggatg atcttaccta
    17581 taataacatt ttctttcaaa ccaagaagag gatctttctt accgcggatc gcagcatccg
    17641 tcaagacacg agttgtttct tggaatgatg cagcagataa gaaactattt gtttctaatg
    17701 atgcttttgt gatacctagt aagacaggtc gagaagttgc aggaacgcct cctgagacta
    17761 gcgtattgta gttttgatct ttaaagtctg cgatgtctaa taatgtacct ggtaagatat
    17821 ctgtatcacc tggatccatt acacgaactt tacgcagcat ttgacgaacc attacttcga
    17881 tatgcttgtc gccgatttct accccttgca tacggtaaac acgttgtact tcacgaagca
    17941 agtagttttc aacagataat acatcgcgaa cttgcaacaa ttgttttgga tcgattgatc
    18001 cttctgtcaa tggtgcacca cggtggatca tatcgccttc tgctactttc atacgtgctg
    18061 tataaggaac agtatacgta cgtgtatctg tttttccttt gattgttact tctttggtac
    18121 gtgttgcagg atcttctgag atatcgatga catcccctgt tacttctgtg atcactgctt
    18181 gccctttcgg attacgtgct tcaaagattt cttggacacg aggaagacct tgagtgatat
    18241 cgtctccggc aaccccacct gtatggaatg tacgcatcgt caactgagta ccaggttcac
    18301 cgattgattg agcggcgatc gtaccaactg cttctccgac ttcaacgtca gagccagttg
    18361 caaggttgcg tccgtaacaa tgtttgcaga caccatgttt tgtattacat gtgaagacag
    18421 aacggattgt cactgtttcg acacctgcat caacgatttg ttttgcgatg tcttcggaaa
    18481 tcaattggtt tcctgcaatg atcaattcat ttgtttcagg atgacgtact tctttacggg
    18541 tgtaacgtcc cactaaacga tcttctaatg attcgatgat ttcatttccc tcgcggatgg
    18601 cttggatttc aagacctcgg tcagtaccgc aatcttcttc acggatgatt acatcttggg
    18661 ccacgtcaac cagacgacga gtcaagtaac ctgaatcggc agtcttcaag gctgtatcgg
    18721 tcatcccttt acgagccccg tgagttgaga taaacatttc caagacggat agtccttcac
    18781 ggaagttcga aatgattggc aattccatga ttcgaccatt aggagcggcc atcaaaccac
    18841 gcattccggc aagctgtgta aagttggaaa tgttaccacg agctccagaa tcagacatca
    18901 tgaagattgg atttttcgct tccaaacctt ccatcagttt ttgttggatg ctgtcttttg
    18961 cagcattcca aacagcaatg acacgttcat aacgttcgtc atctgtgatc agaccacgac
    19021 ggaattgttt tgtaatagtt tcaacttgtt tatgggcatt ttcaatgatt tcttgttttt
    19081 cattcagtac actgatgtca gcgatcccta ctgtgatacc ggcatgtgtc gaatgtttgt
    19141 accctaagtc tttcatacgg tcaagcatct tagaagtttc tgttactttg aatcgtttga
    19201 agacttctgc gatgatgttt cctaagtttt tcttcttgaa cggtaatact aattcttgct
    19261 ctttgatatg tgcaggaata tcagtaccag cttcaacaaa gtatttgtcc ggtgtttgga
    19321 cggtcaagtt gaagtctgtt ggttcattca gataagggaa ttcaggaggc atgatctcgt
    19381 tgaagatgat cttaccaact gttgtgatca tcgtacgttc tttttgccat tcagtaaatg
    19441 gtttttcacc taatgagtta gggttcacac cgatacgtga atgtaaatga acatatccgt
    19501 tgcgccatgc gataacggct tcgttttgat ctctgaagac cattccttcg ccttcgcgtc
    19561 cttcttcttc cattgtcaag tagtagttac ctagaaccat atcttgggaa ggagtaacaa
    19621 ctggtttacc atctttaggg ttcaagatgt tttgagcagc taacatcaac atacgtgctt
    19681 ctgcttgtgc ttcttcattc aatggaacgt ggacagccat ttggtctcca tcgaaatcgg
    19741 cattataagc ttcacataca agtggatgca aacggattgc gcgtccttgt actaaaactg
    19801 gttcgaatgc ttggatacca agtcggtgaa gcgtaggtgc ccggttcagc aatactgggt
    19861 gttccttgat tacgtcttct aaaacatccc atacttcgtc ttcttgacgt tcgattttac
    19921 gtttcgcatt tttgatgttt gaagctagtt cgcgttgaac aagctcacgc atcacgaatg
    19981 gtttgaataa ttcaatggcc atttcttttg gaagtccaca ttggtacatc ttcaagaaag
    20041 gtccaacaac gataacggaa cgacctgagt agtcgacacg tttacctagc aagttttgac
    20101 ggaaacgacc ttgtttccct ttcaacatat gagaaagaga tttcaatggg cggttacctg
    20161 gtccagtaac tggacgtccg cgacgaccgt tatcgatcaa tgcatctact gcttcttgca
    20221 gcatacgttt ttcattttga acgatgattc caggtgcatt caagtctaac aaacgtttca
    20281 aacggttatt acggttgatt acacgacggt aaagatcatt caagtcactt gttgcaaaac
    20341 gtccaccttc taattgaacc attgggcgta aatctggtgg aataacaggg ataacgtcca
    20401 taaccatcca gcttggttga tttcctgaag cgcggaatgc ttctaaaata tccaaacggc
    20461 ggattgcacg tgtacgtttt tgtccttgtg cagatttcaa ttcttctttc aattcggctg
    20521 cttctgcatc tagatcaaca ttatcaagta attgtttaat cgcttctgca cccatagcag
    20581 cttggaattc ttgtccatat tgttcacgtt tttcgcgata ttcgcgttct gtcaacaatt
    20641 gttttttctc taaattcgta tttcctggtt cgatgactac atatgaagca aaataaatga
    20701 tttcttccaa agcgcgtggg ctcatgtcta atacaagacc catacgtgaa ggaatccctt
    20761 taaagtacca gatatgtgaa acaggagctg ctaattcaat atgtcccatg cgttcacggc
    20821 gaactttcga acgagtaact tccactccgc atcgatcaca aacgattcct ttataacgga
    20881 tacgtttgta ttttccgcag gcacattccc agtcttttgt aggaccaaaa atccgctcac
    20941 agaacaaccc ttcgcgttca ggttttaagg tacgatagtt aatcgtctca ggttttttta
    21001 cttcaccgta agaccagctt ctgatttttt cgggagaagc caaacctatt tgcatacttt
    21061 cgaatttatt tacatcgatc aaaaggggtt ccctccattt catttaatgg caaaccgcaa
    21121 gacggctgat cttcgaccgc cttgcagctc atagaccgga ttagtctaaa ttatcttctg
    21181 cggtttcaaa gttttggatg acatcgtctt tttcatcttc cacttgttca gcagctttct
    21241 tttctaattc tttggcagtt tgctgttcgg caaattttgt caaagcatca acagtgatca
    21301 aatcgtcgtc ttcatcgtcc atgtcgcgaa gttcgatctc tgtttcttcg atatccaaga
    21361 cacgcatgtc cagacctagt gattgcaatt ctttcaccaa tacgcggaat gattccggta
    21421 cacctggttt tggaattggt tcacccttga cgattgcttc gtatgttttc acacgtccga
    21481 ctacgtcatc tgatttgtat gtcaagattt cttgtaaagt ataagccgca ccgtaagctt
    21541 ccagtgccca tacttccatt tcaccaaaac gttgtccacc aaattgagct ttacctccca
    21601 atggttgttg tgtaaccaat gagtaaggtc cgattgaacg tgcatgcaat ttgtcatcga
    21661 ccatgtgagc aagtttgatc atgtacatca caccgacaga cacacgtcca tcaaatggtt
    21721 cacctgtacg tccgtcgtac agaacagttt tcgcatcgct ggccataccg gcttcacgaa
    21781 ctgtttccca tacgtcttca tcacttgcac catcaaatac tggcgttgct acgtggatac
    21841 ctaactgacg tgcagccata cccaagtgta attccaatac ttgcccgatg ttcatacgtg
    21901 aaggcacccc taaaggattc agcatgatat cgattggtgt tccatcaggt aggaatggca
    21961 tatcttcttc cggcataata cgggatacaa cccctttatt tccgtgacga ccggccattt
    22021 tatccccttc atggatttta cgtttttgaa cgatataaac acgaactaac atgtttacgc
    22081 ctggtgacaa ttcatctcca gcttcacgag taaagatttt cacatcgtga acgataccgc
    22141 cgccgccatg tggtacacgt aatgatgtat cgcggacttc acgagctttt tcgccaaaga
    22201 tcgcatgcaa tagacgttct tctgcagata attctgttac cccttttggc gtgactttcc
    22261 ctacaagcaa atcgccatct tggacttctg caccaatacg gatgataccc atttcgtcta
    22321 aatcttttaa tgcgtcttcc ccgacattag ggatttcgcg tgtgatttct tcaggtccta
    22381 attttgtatc gcgggcttct gattcatatt cttcaatatg gatagaagtg tatacgtcat
    22441 cttttaccaa acgacggctc atgatgatcg catcttcata gttgtaccct tcccaagtca
    22501 tgaaaccaac taaaacgttt tggcctagag ccatttcgcc ttgttccata gatggtccgt
    22561 ctgccagagt atcccctttt tcgactttct cacccaaatg aacgattgga cgttggttgt
    22621 agcttgttcc ggagtttgaa cgacggaatt ttgttacatc gtatttatct aatgcaccgt
    22681 tgtcgcggcg tacgcggatt tcagaagcat caacgtattc aacgacaccg tcatgtttac
    22741 ataacaaagc agctcctgag tcatgcgctg atttgtattc cataccagta ccaacccatg
    22801 gtgcttgagg attgatcaac ggcactgctt gacgctgcat gttggcaccc atcaaggcac
    22861 ggttggagtc atcgttttcc aagaaaggaa tacatgctgt cgcaacggca actacttgtt
    22921 taggtgatac gtccatgtag tctactttat caatagatac ttctaagttt tcactttgag
    22981 cacgagccat aacaacgtct tgagcaaatg ttccatcttc attcaatggg ctgtttgctt
    23041 gggctacgat gtaatggtct tcgatatcag ctgtcaagta gtcgatttgg tcagtaacac
    23101 gacctgtttc acggtctaca cgacgataag gtgtttcgat aaatccgaat ttatttactt
    23161 tcgcataact ggacaaacta ttgatcaacc cgatgtttgg gccttcagga gtttcgatcg
    23221 gacacatacg gccatagtgg gagtagtgaa cgtcacgaac ttcataaccg gcgcggtcac
    23281 gagtcaatcc gccaggtcct aaggctgaaa gacgacgttt gtgtgtcaat tcgccaagag
    23341 ggtttgtttg atccatgaac tgtgacaact gagaagaacc aaagaattct ttgatacttg
    23401 ccacaactgg acggatattg atcaattgtt gaggtgtcaa tgtttcagta tcttggatcg
    23461 acattctttc acgaaccaca cgttccatac gtgctagccc gatacggaat tggttttgta
    23521 aaagttcacc gactgaacgg atacgacgat ttcctaagtg gtcgatatca tcgacattgc
    23581 cgataccttc catcaagttc aagaaatagc tcattgaagc aatgatgtcg gctggacgaa
    23641 ccgtttttac agctgcttct ggatagccgt ttccgatcac gtttacttca cgttcagggt
    23701 ctcttggtga aaatactttg atgacttgga cagtcattgg atctgtgacc acaccgtctt
    23761 ctgatggata gtaagtcact gaatttaggc cattatcaat gaaaggagct agtgtttcca
    23821 tgacttggtg agtcagaact gttccttttt caacgatgat ctcgcctgtt tctggatcaa
    23881 ctaatgtttc agctaatgtc agattcaata gacgtgtttt caaatctaat tttttgttta
    23941 ctttgtaacg accaacgttt gctaagtcgt aacgttttgg atcgaagaaa cgagcattca
    24001 gcaagttacg tgagctgtct gctgtttttg gttcgcctgg acgcaaacgt tcgtaaacgt
    24061 ctttcaaacc ttcttctgtt cttgagtcgc ttgcgttttt atgcaagtct ttttcgactg
    24121 tattgcgtaa tgtttcgctg tcgccgaaga tttcaaagat cgtatcatcg gaaccaaatc
    24181 ctaacgcacg aactaatacg gttaaaggaa tcttacgtgt acggtcgata cgtacataag
    24241 agatgtcttt tgcatcagtt tccatttcta gccaagctcc acggttaggg ataacagtag
    24301 aaccgaatcc ttcttttccg tttttgtcga cttttccatg gaaatagaca cctggtgaac
    24361 ggactaactg agaaacgatt actcgctctg ccccgttgat gatgaatgtt ccttgttctg
    24421 tcattaatgg gaaatcaccg aagaatactt cttgtgcttt gatttcgcca gtctcgcggt
    24481 ttgtcaaacg caatgttaca tgtaatggtg cagagtagtt tgcatcatgc gcacgagctt
    24541 ctgctactgt atactttggt tctttcaatt cataatctac aaattccaaa gatagattgc
    24601 cattgaagtc atcgattggc aaaatgtctt caaacatttc tctaagtccc tcatctaaga
    24661 accattggta agagtctgtt tgaatttcga tcaaatttgg taattccaac acttcactga
    24721 ttcttgcgaa acttctacgt tcgcgatgtt ttccgtattt tactacgtgt ccagccaagc
    24781 tcttcacccc tctaaaatgt cattcaaaaa gactacccga tcaattgtta caataatatt
    24841 tcaaatatta tttttaggta acaacagaca ttttttgcgg cttttaggca aaaaaaaacc
    24901 aaaaatagaa cattctcttg aaaccttact tcgtgagctt ctacttttgt ttccttatat
    24961 taaagccgaa acggacaata tttcgtttct gccttttctt catcagatag tttttgcaac
    25021 ttcatatact acacttattt tctataaaag tcaacacttc ctggcgtttc ttctgctttt
    25081 tcgcaacatt ttcttaatgt tttcaatttt atgttttcaa ttatctgaca attcttaatt
    25141 gacagacctt tttatctcgg ttatacttta gaaaattcat tttgtggagg aacttctatg
    25201 cgaaccaaca tcaaccaaat cgcaccaaca caactgaata acttttactt tagctacttt
    25261 agataggttt gtattcatca tgatggatac aaacgttaaa cgacagtttg tatctatgat
    25321 ggctaaagaa ctttgtattg cagcaaaaag ccacgtagac aaaatgctaa gtggctgagc
    25381 agtgaaaata caaagtaaaa gtatcaagca ttctactttt tcaatttatt ttccgacttt
    25441 tcaatccatt tggacatttt gtctgaatgg attttttgtt tcccaaaatt aaggaggaaa
    25501 aagaaatgaa aaaaaatata atcatcggat taggcgctgc cctgctacta gctttaggag
    25561 gttgcgcaca aaaagacaat tccgcagagg catcagatgg acaaaaaaca attggcgtat
    25621 tgcaacttgt acaacataac tcgttagatg ctgcctataa aggattcaaa gaaggattag
    25681 cagaagaagg gtataaagaa ggcgataatc taacaatcga ttatcaaaat gcccaaaaca
    25741 atcaagacaa cctgaaaagt atgagtgaga aactcgtcaa agaagatcct gatttgctac
    25801 ttggaatcgc cactccagca gcacaatctt tattaaatga aacaaccact atcccgatga
    25861 ctgtcacagc ggtcacagac ttggaacaag cccatttagt cacatccaat gaaaaacctg
    25921 gcggcaatgt gactggcacg acagatatgg tgccaatcga aaaacaaatc ggcttactgt
    25981 taaatatcgt gccagacgcc aaaacaatcg gcatcatgta taacaacggt gaagcaaact
    26041 ctaagatcca agctgatttg gcagaaaaag aattgaaaaa agcgggcgtc aaagtcaaag
    26101 tactgacagc aaattccaca aatgatgtcc aacaagtgac gaaaagtcta gctaaagatg
    26161 tagatggaat ctatattcct actgataaca cgttcgcaag tgcggcaagt gttgtcggtg
    26221 aagtcgcaaa agaaacaaaa acacctgtcg ttgctggcgc agttgatcaa gtcaaagacg
    26281 gcggtctagc aacagtagga atcgactacg aaagcttggg taaacaaaca gggatcatgg
    26341 cagcaaaaat cttagacggc aagaatccaa gtgaacttcc tgtcgaaaca gcgaatgatt
    26401 tggaactgta tgtcaataaa gacatggcca aagcattagg catcaatcca gacagcatca
    26461 aagaagctga ataaaacaca ggaggattct catgacaaat ttgctctctc ttttcttatc
    26521 atctgcatcg caaggggtct tatgggccct acttgcgatt ggtgtatacg tcactttccg
    26581 tatcttggac attgccgact tgactgctga aggaagcttt cctttaggtg cagggatcgc
    26641 ggcagtaagt attacaaatg gctatcatcc attgacagct tgtcttctag gctttttagg
    26701 gggtgcggca gcagggcttg tttccggact gcttcacaca aaattgaaaa ttcccgccct
    26761 tcttgcaggg atcatcacca tgactggttt atattctgtc acatcccgtg tgatgggtgc
    26821 gccgaacatc tctttattgg gtcagccaac gatctttcat tgggtcgagt cattcggcat
    26881 aacaaaagac aacgctgtac tgatcgtcgg tctgcttttt gctttgatcg ttgtatgttt
    26941 acttgttttg ttcttacgta cagaaacagg acttgctatt cgtgcaactg gcgataatct
    27001 ggcaatgagc gaagcaaacg ggatcaacac agataatatg aagatcatcg gttatatgat
    27061 ttctaatgga tgcattgctc tttctggctc cttgcttgcg caaaacaacg gctttgctga
    27121 tttaaattct ggtattggaa cgatcgtaat tggacttgct tctattatca tcgcagaagt
    27181 tttgttacgt aaccagccac tatttctccg tctcttgact gtcgttttag gtgcggtcat
    27241 ttatcgtttt attttagcgt tagtcttcga actgaatgtt gaaccaagtg attcgaaact
    27301 tgcttctgct ttcgtgctag tgatctgtct ttctttcccg cagatccagc aaaaattcca
    27361 tttacctcaa aaaaagaaag gcggtagtgt caaatgaatg acgtcttaac attggaaaat
    27421 ctccatcata cctttgaaaa aggaacgatc aacgaaaatc atgtcctgaa aggcattaat
    27481 ctttccttag atcccggcga cttcgtgacg atcatcggtg gaaatggggc tggtaaatct
    27541 actttgctga acagcatcgc cggtacattt tctgtcgatc agggtcaaat tttattgaat
    27601 ggaaaaaata tcacgaaaaa atctgttgtc gaacgttcga aatccatcag tcgcgtattc
    27661 caagatccta aactagggac agccgttcgt ttaacggtag aagaaaatct tgctcttgct
    27721 atgaaacgag gaaaaaaacg cggcttcttc cgtggtgtca aaccacaaga ccgaacattt
    27781 ttcaaagacc agctggttcg gctaaatttg gggttggaaa atcgtttatc ctcagaaatc
    27841 ggtttgctgt ctggaggaca acgtcaagct attacgttat tgatggccac attacagcgt
    27901 ccagaactga tcttattaga tgaacacact gcagcgcttg accctgctac ttccaaaacg
    27961 gtgatggaat tgacagaaaa gctgatcact gagcagaatc tcactgcttt tatggtcact
    28021 catgacatgg aagacgcgat ccgctatggg agtcgactga tcatgctgca tcaaggaagg
    28081 atcgtcgtag atatccagca agaggaaaaa cagcagctca cagtgcccca attattagcg
    28141 ttgttcaaac aaaacagcgg cacggcatta aaagacgatc aagtactttt atcttcataa
    28201 tcagaaagag gatgggacaa aaaatgaacg cccaccctct tcttttttct ctttccttct
    28261 ttcgtttttg ctttatgtat caaccgttgt ttcttcgaaa actattttgt tctctccttc
    28321 ttcctgatag aatggatggg aaaggagctt gatgccatga caacgaaaga caaggtactt
    28381 caattattga aaaagacacc agattttctt tccggagaaa agttggcaca gcaactggaa
    28441 gtttcccgga caagtatttg gaaagccatc aaagaattgg aaaaggcagg ctatcgtttt
    28501 gagcacgggc ctacagggta tcgttacctg ccctctgacg tactagatgc aaaagaaatc
    28561 cagcaagagc tacggtcgtt tattcctgat ttgaaagtac aaacgatgtt gacttctgaa
    28621 tctacgatga aagatgccaa actggcagca gctcaaggag attctgtttc gaagttattt
    28681 gttgctgata cacaagaaca agctcgtgga cgttttggac ggcctttttt cgctgaacct
    28741 ggacgaggaa tctacatgag tctcttgctc catccagaca aacggtttga tgaattgcct
    28801 caatatacga tcatcgcagc cagtgcctgt gtgaaagcca ttgaatctgt aaccggtcgt
    28861 tctgcttcga tcaaatgggt caatgatatc tacatggatg gaaaaaaagt ctgtgggatt
    28921 ttatctgaag cagtcagtga tatggaaagc ggcaggatca gtcatatcat cattgggatc
    28981 gggcttaatt tttctgttcc tcaaaatcat tttccagctg atctccaaga aaaagccatc
    29041 tctgtctttc ccgacggaca agctagtgtc acaaggaatc agctaatcag caaaatctgg
    29101 caagaattgt tcacttatct ttctcaccca gaaaaagcgt tggacttcta taaagaaaaa
    29161 tcattcgtat tgggaaaaac cgtgtcgttc aatcaaaaag gaacgactta tgtcggtctt
    29221 gcaaaagaaa tcggtgatca tggcgaactg atcgtccaaa caaatgaaca gaagatgagt
    29281 ttgtcttctg gcgaaatcag tctcacatct atccgtacgc aataattatc agatttcaca
    29341 aaaggacacc tgatctcata cagtgtcctt ttttctgaat tctcttcaaa agactctcct
    29401 tattctatca gctgacagtt tctctatcct ctagaaaaag gtattctaaa aatatctata
    29461 aagcatggta cactttcttt aaatgaatca atgcaaaaaa caagagagtc atcagtttac
    29521 acgatcaaaa gagatagcag gccgcttcct ttttcaatct tatagataga ccatgggtaa
    29581 cgataaaaac aaaaaagaac ttgcgactta ttttgccgta agttcttttt tagctagtgc
    29641 cttaatctct taatcccaac aaacgtctcg catagatcac ctgaccagag tgcatagcag
    29701 catcatcaat aatagaaacg agacgcattc ctcgtgtgac tggtggtgtc cagttctcgt
    29761 caattacttc atctagctcg gaaggttcca gccttcttaa gtaagaaata gcaaaatcag
    29821 cagctcctcg caagtatcct agcagatctt ctatttgatt agcttggatc tgctttgctt
    29881 gttccagact atgcttccaa tcttgttcgt tatctgctat cgtcatcgga aaccggcttt
    29941 cccagtcttg actatgccaa attggagaag ttcctgctaa atcagcaatc tggaaatcta
    30001 attctcgagc ggtatgccaa gccagccaag tcattgattt gatctgcggg gctctatctt
    30061 ccactggaaa acgattggcc tcctcaatag agacgccttc aaacaaacgt tcaaaccgat
    30121 cgtatgcccg atccacactc tcgcatagca tatctaaata atccatttat tttcctcctt
    30181 tttatttatc ctacttactt tttcctaatt ctaggatacc tacttatatg ttactacaca
    30241 cccttatttt ttacacttta acagtcacca tcatcaatag tttggagatt gaaagaaaaa
    30301 gatagtgtcg aactatcaat tgaacaagaa gcaacaatat gaacgtttca ttaatattct
    30361 ttaaataaac aatgattttt caaacgaaca gtttaagact aggagaaaaa gagtataata
    30421 ggaaaatgtt tgaaaggaaa ggaaggggaa aaatgaaaaa aagggcaagt ttactagtcc
    30481 tagttgcttt gttgttcaat atcttttcgt atggtgtggc cgtttcagcg gaaacataca
    30541 ttcctaaaga ccctgggaat atttataatg atctaactgg taactatggc ttgttaggaa
    30601 ttgctagtca attccacgta tttgctaaaa ataagacaac tgtgaatgca catacagacg
    30661 gaaatatagc aacacatgaa cttgatgcaa aaaataattt tggtacagag cttcatgaag
    30721 gtttattgaa ccaagaaata aattatatac aaaaaactga ttctttcgct aattcttcag
    30781 gcatacctac aggtagcaca agaatgaata aatttgtcgt tggcagtaac gtggatgtag
    30841 cacttcaaga tggtcatcct acagtcaatg gatccaggct tgatcactta actccagatg
    30901 atttttataa agatcgtgct gggcatgaat atattgattt cgagcaggaa tttgctaaat
    30961 tgaatgtagc agcaaatgat cttgcaacga tcactccagc taaaacatat actgctgcag
    31021 attttccaga catgaacaat cggacaatcg atttaactga tttatcagat accgggtttt
    31081 tattggtaaa tatcgatgca gaagtgttaa cgatgaatac gccacttcaa atcattaatc
    31141 caaatgatca agtagttgta tttaatgtca tcaacagttc ttctgcattg aacgtacagt
    31201 caccaatcaa atacaatgat cgttcaaatc atgaaacaga agatttttcg gatgcgaata
    31261 tctcttggaa ttttggaaat gagatgactg atttgacaat cagtgctcct tttcaaggaa
    31321 caatccttgc gcctaatgct actatccgca ttactcaaaa ccaagacgga acgattatag
    31381 gaacaaatgt gattttagat gcagctacca atcgttggga tcctaacgaa atatttatca
    31441 gcgacaatgg tacagatacg acagacacaa gcgacagtac agatacatct accacaagtg
    31501 atagctcaga ttcatcaact tcaagcgaca gtacagatac tacctctacc acaagtgata
    31561 gcacggatac atcagcttca agcgacagta cagatactac ctctaccaca agtgatagca
    31621 cggacacatc agcttcaagt gacagtacag atactacctc taccacaagt gatagcacgg
    31681 acacgtcagc ttcaagcgac agtacggata catc
//
LOCUS       DACECZ010000018        30949 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.018, whole genome shotgun sequence.
ACCESSION   DACECZ010000018 DACECZ010000000
VERSION     DACECZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.018"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(71..790)
                     /locus_tag="IWO35_000826"
     CDS             complement(71..790)
                     /locus_tag="IWO35_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0315902.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(921..2072)
                     /locus_tag="IWO35_000827"
     CDS             complement(921..2072)
                     /locus_tag="IWO35_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ0315903.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(2289..3029)
                     /locus_tag="IWO35_000828"
     CDS             complement(2289..3029)
                     /locus_tag="IWO35_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0315904.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3033..3356)
                     /gene="rsfS"
                     /locus_tag="IWO35_000829"
     CDS             complement(3033..3356)
                     /gene="rsfS"
                     /locus_tag="IWO35_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ0315905.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(3413..4006)
                     /locus_tag="IWO35_000830"
     CDS             complement(3413..4006)
                     /locus_tag="IWO35_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0315906.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(3990..4637)
                     /locus_tag="IWO35_000831"
     CDS             complement(3990..4637)
                     /locus_tag="IWO35_000831"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ0315907.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(4650..4961)
                     /gene="yhbY"
                     /locus_tag="IWO35_000832"
     CDS             complement(4650..4961)
                     /gene="yhbY"
                     /locus_tag="IWO35_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ0315908.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(4977..6086)
                     /gene="yqeH"
                     /locus_tag="IWO35_000833"
     CDS             complement(4977..6086)
                     /gene="yqeH"
                     /locus_tag="IWO35_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ0315909.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6083..6610)
                     /locus_tag="IWO35_000834"
     CDS             complement(6083..6610)
                     /locus_tag="IWO35_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ0315910.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(6790..7575)
                     /locus_tag="IWO35_000835"
     CDS             complement(6790..7575)
                     /locus_tag="IWO35_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ0315911.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(7568..8437)
                     /locus_tag="IWO35_000836"
     CDS             complement(7568..8437)
                     /locus_tag="IWO35_000836"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ0315912.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(8438..9817)
                     /locus_tag="IWO35_000837"
     CDS             complement(8438..9817)
                     /locus_tag="IWO35_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ0315913.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(9825..10244)
                     /gene="fabZ"
                     /locus_tag="IWO35_000838"
     CDS             complement(9825..10244)
                     /gene="fabZ"
                     /locus_tag="IWO35_000838"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0315914.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(10269..10745)
                     /gene="accB"
                     /locus_tag="IWO35_000839"
     CDS             complement(10269..10745)
                     /gene="accB"
                     /locus_tag="IWO35_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ0315915.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(10748..11983)
                     /gene="fabF"
                     /locus_tag="IWO35_000840"
     CDS             complement(10748..11983)
                     /gene="fabF"
                     /locus_tag="IWO35_000840"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ0315916.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12002..12739)
                     /gene="fabG"
                     /locus_tag="IWO35_000841"
     CDS             complement(12002..12739)
                     /gene="fabG"
                     /locus_tag="IWO35_000841"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ0315917.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(12751..13668)
                     /gene="fabD"
                     /locus_tag="IWO35_000842"
     CDS             complement(12751..13668)
                     /gene="fabD"
                     /locus_tag="IWO35_000842"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0315918.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(13775..13999)
                     /locus_tag="IWO35_000843"
     CDS             complement(13775..13999)
                     /locus_tag="IWO35_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0315919.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14052..15014)
                     /locus_tag="IWO35_000844"
     CDS             complement(14052..15014)
                     /locus_tag="IWO35_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ0315920.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15024..15464)
                     /locus_tag="IWO35_000845"
     CDS             complement(15024..15464)
                     /locus_tag="IWO35_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0315921.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            15770..16735
                     /locus_tag="IWO35_000846"
     CDS             15770..16735
                     /locus_tag="IWO35_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0315922.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(16792..17022)
                     /locus_tag="IWO35_000847"
     CDS             complement(16792..17022)
                     /locus_tag="IWO35_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ0315923.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(17108..17995)
                     /locus_tag="IWO35_000848"
     CDS             complement(17108..17995)
                     /locus_tag="IWO35_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ0315924.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTNTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(18084..19307)
                     /locus_tag="IWO35_000849"
     CDS             complement(18084..19307)
                     /locus_tag="IWO35_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0315925.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(19324..20403)
                     /locus_tag="IWO35_000850"
     CDS             complement(19324..20403)
                     /locus_tag="IWO35_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0315926.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(20533..22260)
                     /gene="ptsP"
                     /locus_tag="IWO35_000851"
     CDS             complement(20533..22260)
                     /gene="ptsP"
                     /locus_tag="IWO35_000851"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ0315927.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(22260..22526)
                     /locus_tag="IWO35_000852"
     CDS             complement(22260..22526)
                     /locus_tag="IWO35_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ0315928.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            23005..25239
                     /locus_tag="IWO35_000853"
     CDS             23005..25239
                     /locus_tag="IWO35_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0315929.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            25387..25707
                     /locus_tag="IWO35_000854"
     CDS             25387..25707
                     /locus_tag="IWO35_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0315930.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(25912..26135)
                     /locus_tag="IWO35_000855"
                     /pseudo
     CDS             complement(25912..26135)
                     /locus_tag="IWO35_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(26333..27913)
                     /locus_tag="IWO35_000856"
     CDS             complement(26333..27913)
                     /locus_tag="IWO35_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ0315931.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(28314..29690)
                     /locus_tag="IWO35_000857"
     CDS             complement(28314..29690)
                     /locus_tag="IWO35_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0315932.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(29818..30936)
                     /locus_tag="IWO35_000858"
     CDS             complement(29818..30936)
                     /locus_tag="IWO35_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0315933.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
BASE COUNT         8689 a         6686 c         5311 g        10263 t
ORIGIN      
        1 aagacaatct gttagtgaga ttgtcttgct tttcgaaaaa gactgaatac ttttcgttaa
       61 aactggttgc ttagtcttct aatgccacat tatggaacac atgctgtaca tcttctagat
      121 cttccagtgc atcaatcatt ttttcaaatg tttcttgatc ttctggagat agtgttacct
      181 cgttttgagg gatcatttgg atctcagcga ttgaaaaatc agtgattcct ttttctttta
      241 atgcttcttg tatactatgg aaatccgcta cttcaccgta aacgatgatt tgtccttctt
      301 cttccattac atcgcgaaca tcgatgtctt tttccattag gtattcaagg atctcgtcag
      361 cgtcttctcc tgcaaaagca aagattgctg tattgtcaaa catgtaagaa accgctccgg
      421 aaacacccat gttcccgcca tttttcccaa atgctgcccg tacgtctgct gctgttcgat
      481 tcacattgtt cgtcaatgta tcaacaatca ccattgagcc gcttggtcca aatccttcat
      541 agcgcaattc agagtatgtt tcatctcctg caccttttgc tttttcgatt gctcggtcaa
      601 taatatgttt tggtacatta taagttttag cgcgatcaat gacgaaacgt agtttttggt
      661 ttgcatgcgg atcaggatca cctgattttg ctgccgcgta gatttcaatc ccaaattttg
      721 cataaatccg gctattatta gcatctttag cagttttctt tgctacgata tttgcccatt
      781 tacgtcccat attttcactt cgctttctga tttgatagtt tcataacgca aaaacgtcct
      841 gttccattat acttgtgaat cacgattctg tgaagctttt tttctttctt ccttcattct
      901 tctcattttt agtcaattat ctatactcgg attgggattt gcccgaaatt ctgttccgga
      961 atattgcctc ccaattgata gatttgatca cttctaacta acagttccgc ttcttgtgcc
     1021 tctttttttc cgattcggga ataaatagaa atctcttttt tttcttttaa gaatcttctt
     1081 ccagcatcgg taaatccaag tagtcgaaga taatttttat tttgttggac aggaatctct
     1141 tgctttttga tattcagcaa gacataacaa gataatcgtt ggatccgtgt ccatgtatat
     1201 ctttttgttt ttacctttga cacaaaatcc tcaaaactat cagcagttgc agctgctgtt
     1261 ttcatgcgcg attctattcc ttcggtcatc tgatagatat ctgtcagttc cagcaaagta
     1321 gaagataaca accgataacg caaatacggc caataagatt cccaagtctg cagcgaacaa
     1381 gttgaaagat cttcttctgt ctgaggcggc acaagtccag ctattctttt tccttcttct
     1441 aacgcatggc gaatggctgt tgcactggct atcctctgat ctgtgatgat tttttcatga
     1501 tagcctgcac ttttacgtcg gatcggatgt aattccatag ggtggacata cttggcattt
     1561 tcctttgcat aacttaatcc caagatatga tttggcgaag aaaaatcaag cgtaatatcg
     1621 ggatacagac tgcgaaaaac ctcagtcatt ttttgcggat aactatacgt ttgatcagac
     1681 agttcttgaa atgcctcttc tatcctatct tgattctctt ggacaaagcg tccgaaactg
     1741 ctgtaatcaa acgaagtctc ttcttctgtt ccaaaacaca aactgtcaca gtccagtgtt
     1801 tgcaagattt ttactgcacc tttagcaaaa tagtcagctg actgaaccgc ccattcgacg
     1861 ggaagctcca ctactaaatc cacaccattt tgtaaagctt cattcgcgcg atgccattta
     1921 tcgatgatag ccggttcgcc tctttgcaaa aaattcccgc tcattacagc aatgacgacc
     1981 tctgctcctg ttatttttcg tgccatttcc acatgatagc gatgtccatt gtgaaaagga
     2041 ttgtactcta cgataatccc gcagcttttc atgcgcatcc tccttcatag ttatcgataa
     2101 aaaagaaata gagacaagta aaaaaaggca aaaatcggaa aaaatacccg aactatataa
     2161 actattctcg caaacacaat acttctgcga cgaaaagctg cactgcaaga gcaatgccta
     2221 tatcgttcgg atatataatt tatgttgaag atttattctt ttcttgttaa ctcgagcagg
     2281 caaaaccgct agtctttgta tgctacaaag aaccagcgct ttgatttctc tgatgctgct
     2341 tcgtctgtaa aatcagaaaa tgcttcgact ttcgaaaagc cgctgttttc cagcattctt
     2401 aaataacttt ccaatggata ggtacgctct tgatgtagtt catcttcacg aataaatcga
     2461 ccagaccctt cttgttcttc aacgaaaaag gtaagaaaat gttcgatgct gtgtttctcg
     2521 cttcctggat aactatccca caaaaaagca aaattatccc cttgataatg atagctatat
     2581 tctggaaaaa cctcatctac ttgataggtg gagtgtacgt caaataaaaa tcggccattt
     2641 tcttctaacg cttgatagac ttcatcaaac acttgctgta cctcttgtct atttttcata
     2701 tagcacagcg aatcagaaaa acaggtaatt gcctgatatt gtccgatttc tgataaatcg
     2761 agcatattgc cttccacaaa ttggatcgat gcgtcttctt catatgctcg ctggctggca
     2821 atcatcaaca tctcttctga aaggtctaac cctgtcactt tatatccatc ttttgcaaag
     2881 gcaaccgcta atgctccagt accgcatgcc agttccaaaa tattttttgt gtcttcttgt
     2941 aaatgacgtt tcgaaaaagc taaccattgc tgataaagtg tttcgtccat cacttcatcg
     3001 taaatgaaag caaatgtttc ataagccata tgttaatcca accattcact aagatcgact
     3061 agcggagcat ctgaccatag tttttccaga ttatagaaat ttcgttctgg cgcatggaaa
     3121 acatgtacga tcacatctcc aagatcgatc aatacccatt ttccgccttc ttttccttca
     3181 atacgtttta cttcatattt gttttcttct tctttatcaa tgatttcttc tgtaattgcg
     3241 ttgatttgac gttcgctatt tgctgaacag atcatgaaat aatctgccaa caaggaaaca
     3301 tttcgaacgt ctaaagcgat aatgtcttct gcccgttttg aatctgctgc ttcaatcgca
     3361 atttttaatt gttctttact atctatgatg atttcctcct aatatgagca gcttattttt
     3421 ttgctaccca gtaattatat gtttcgattg tttttggata aatctgttgt tcttgcgcaa
     3481 gcaaatgggc caatgtatgt tttgtttcaa atgctacagc ttcgtctaga tcagcccatg
     3541 caatggctcg tgcatcttgt accccaggaa aatctcgacc tggttcaata tagtctgcta
     3601 cataaatgat tttgtcaagg agactcatct tagcagcccc agtcgtatgc acccggattg
     3661 catgaagaat ctctgcatcg gtgattccta attcccgctc tacaaacgaa gcgccaacaa
     3721 gaccatgcca gattgcatta ttataattca ataactccgg atcaaaaccg tctcttacga
     3781 ttaccatttt gaactcctca tctggtcgtt cttttgcata atcatgtgtc aatgcagcaa
     3841 tactagcttt ttcaggagaa gcaccgtatt ttttggcaag agccacggct gtttcttcta
     3901 ctccaagcac atgcttgaag cgttgttcgc tcattctcat ctgtattttt tgcatcaggt
     3961 tttctctaga ataagcagta tattcattac tatagtccat attcatacaa cccttcattt
     4021 tgaatataat caattacttt atctggaaca aggtagcgga tagaacagcc ttctttgatc
     4081 ttttgacgta ttttcgtgga actgatgtcg atctcaggta catctaccca aattacagga
     4141 tatggagtat ccgttgtgta tcctggtcga cgtataccca caaagttgac catagaagtc
     4201 agctcatcga ttttatacca ttttggcaaa tattctacca tatccccacc gataataaag
     4261 taataatctg tatccggatt gttttgcgtc aattccttca tagtatcata ggtgtaactt
     4321 ttcccaccac gagctaattc aatcgtttca atctgcaaaa aaggattgtc ttccaccgct
     4381 aattccaaca tgttgagtcg atgttttgca tcaatcgtct gtttctcatc aacatgaggt
     4441 ggctgataag aaggcatcaa aaatacgcgg tcaagaccta aattccggcc agcttgttca
     4501 gccatcacta aatgtgcaag gtgaacggga ttgaatgttc cgcccaagat ccctacttgc
     4561 tttttcttct ctaagaattg aggcatttct tcaggaaata attgtgaacg aacaaatgtc
     4621 ttagcttgcg tattcatcat ggtcactcct tatattgctt ttacttcttt tgatagcctt
     4681 tgatactttt cttttgtgga aggtttgtat aatacaataa cacgtccaat gatttggacg
     4741 atttcgcaat ctatttcttt ttctaatact tgagctgctt cttctgcaat ctcatctgta
     4801 ttttgaagta acgatacttt gatcagttct cttttttcta aagcttcttc aatttgaacg
     4861 atcattgctg aattgatccc accttttccg atttgaaaaa tcggctgaag atgatgcgct
     4921 ttgctacgta aaaaacgctt ttgttttcct cttaaactca taattttcct tcttccttaa
     4981 atcaatgctt tgcgtttcaa tacatctaca ccctttggcg cccagcccgc aattactact
     5041 ggcgctgtta cagtgatcca gcctagaccg gcaaaaacga tgtctgtttt ttctttcaca
     5101 gaaaactcaa aacgaacaag ctccggaaat gcctcgactt catcaggacg aggaggctgt
     5161 aataatccac ctacatgttt ttgataaaat tcgtctgctt tttccagttt cgttcgatga
     5221 atatttacat catttgacac ataagcaata aatgaagcac gattgccgct gataaaatca
     5281 aatcttgcaa gtccgcccaa gaaaagtgtt tgcccttcat tcagctgata aactttaggt
     5341 ttgatttctt ttaacggtgc aatgatccgc aaatcttttt tacctaagta gtgcgccatt
     5401 tgatggcgat ggataattcc cggtgtatca atcaaaaaat gcccgtcatc taaaggaatc
     5461 tcgattttat ccaaggtcgt tcctggaaac tgcgaagttg tgattacatc ttgtactccc
     5521 gctgtatttt tgatgatttg attgataagc gtcgattttc cgacatttgt tactccgacg
     5581 atatagacat cccgtccatc gcggtattct tcgatttttt ctaatagttc cgtcatctca
     5641 tgagatttct tagcactagt taataataca tctactggac gaattcctgc ttcgtaagca
     5701 cgttccttca tccattgtgt taatttagtt tttttcaatg atttaggtaa tatatccact
     5761 ttgttcccaa ctaaaagtac tggattgtca ccgacaaaac ggtgtagtcc tgggattagt
     5821 gaaccattga agtcaaaaat atccaccaca ttgacgatca atgcatcttt ttgccctaat
     5881 tcattcagta agcgcaaaaa gtcgtcatcc gtcaatgaga catcttgggt ctcattataa
     5941 tggcgtaatc tgaaacaacg ctgacaatag acttccccgg aagctaaacc tttttccaaa
     6001 gccgtttgcg gcgtgtatcc taattcaccg ggtttgtttg tttgtataac ggcaccacag
     6061 ccgatacatt gtaattcttc tgtcattcga ttccacctcg ccagcccata tcagggtgtt
     6121 ttgctaatag ataggccata attctttttt cccaaaaacg attaaattgt gtcttccaac
     6181 tatctgttgc gataatgggt tttacaagga tgctacgtat ccctgcacga ttggctccac
     6241 gtatatcagt cataatctga tcgcctacca ttactatctc atcatctgct aaatccaatt
     6301 gtttctttgc tatgttgatc cctcttgcta aaggtttcat tgctcgtgca acatacaaaa
     6361 gatcaaattt ttcaatcgct cgcgcgacac gttttgaatt attgtttgaa acaacaacaa
     6421 cagggattcc tgcatttttc atttctaaaa tccaagtaag caactcttct gttccatctg
     6481 gattattcca tgcaatcaac gtattgtcta aatcggtcaa tactgctttg atgcctagat
     6541 tctttaattg ggcaggtgta atctgataga tggcttctac catccatgtt ggtttgtaat
     6601 ttgaaaacat gctgtctcct catctgcaaa aaatgaagga gcacataaag tactcctcaa
     6661 ccccgtatta tactgcatat cagcaaaaaa aactacctct atactcgttc gtctttcaga
     6721 aaaagcattt ctttacaaaa tgaccaaaaa taaagattga tcacagaaaa atttagaaac
     6781 aaaaaacaac taatattggc ggaacctctt gtatcgtttt tccagtaatt tatgctgatc
     6841 taatcgaaca agttccgtca atctttcaat caacgctttt ttcataatgt gtgcgacttt
     6901 atatcgtggc aactgctcgc cgttgaacgt ttcaggaata actcgatcaa cgattcctaa
     6961 ctcgcgtagt tctggcgcag taattttcat caactcagct gcttctgcgg cgcgactacc
     7021 gtctttccac aaaatcgacg caaatccttc tggagaaagc acagcataaa tcgtttcttc
     7081 cagcatccag acttcatccg ctaaacctag agcaagggca ccgccacttc caccttcacc
     7141 aatgatgatg gcgatgatcg gtacctttaa atcagacatt tcgagcagat ttctggcaat
     7201 agcctctcct tcgccgcgct cttctgctcc tacaccgcaa tatgcacctg ctgtattgat
     7261 caaggtaatc accggtctat tgaacttttc cgcttgtttc atcagtcgta aagcttttcg
     7321 atagccttca ggatggggag aaccaaaatt tctgcggata ttttcctgca gatcccgtcc
     7381 tttttgaata ccgatcactg ttaccggttt gccatctaat cgagcgattc caccaacaat
     7441 cgcttcgtca tctgcaaata aacgatcacc atggcattcc tcgaaagaat cgaagatctc
     7501 ttcaaaaaaa tccaagcttg tccatcgttc tttagctctt gctaactgaa cgacatcatg
     7561 tgctgtctta tccacgatct atccatcctt tctgtgtatg caattcaaga agcgtatgga
     7621 ttttctgttt caattccatg cggggaacaa tctgatcgac aaaaccatga gataataaga
     7681 attcagcctt ttggaaatct tctggaagtt cctgcttgat cgtctgttcg atgacgcgtc
     7741 ttccagcaaa accgatcaag ctttggggtt ctgctagaat aatatcgcct tccattgcaa
     7801 aactggcagt tactcctccg gtcgtcgggt ccgttaaaac agttagataa aaaagacctt
     7861 cattgctatg ccgtttgaca gcagcagaga tttttgccat ctgcattaga gaaaagattc
     7921 cttcttgcat ccgtgcgcca cctgaagctg tgaacaagac gactggaaga ctttcctttg
     7981 ttgctcgttc gaataatctg gtgatctttt ctccgacaat cgttcccata cttcccatga
     8041 taaaattagg atccatcact ccaataatga agggaatccc ctgaatggtt gctttaccag
     8101 tgaggactgc ttcatgaagt cctgttttat cttgcatttt tttaattttt tccgtatatt
     8161 cgggaaatgc tagcggatct ttggttacaa gatccgtatc ccattcttca aaactttttt
     8221 catcaataat caaagctaga cgctgccatg caccgattcg aaaagcgtat ccgcaatgag
     8281 ggcatacttt ttcttcaccg atgtctttcg tataaaggat atgtttgcaa tttggacact
     8341 ttgcccacat gttatcagga acagatggcg agttagcagg tttgctctct ctattaggat
     8401 tgatccgaat atatttcttt ttcttaaaca gagccatcta tgcgcctcct atctccgttt
     8461 ctggtgtcca attcggtaaa aattcttctt gtaaaaatgc tgtgctgtaa tcacctgcta
     8521 tgactgcagg atgactaatc aaatccatct gaaattctgc gttcgtgatg attccttctg
     8581 tgataaattc acttaaggct cgctgcatct tcattaatgc ttcaaaacgc gtatttccat
     8641 ggacgattat ttttgcaatc attgaatcat aatatggcgg gatcgtatat cccgggtaaa
     8701 ctgcactgtc tactcgcagt cccatcccac ctgctggaag cagtaagttt tggatcttcc
     8761 ctggagaagg agcaaaatgg aaagcaggat tttctgcatt aatccggcat tcgattgcgt
     8821 gtccttggaa tactacttct tcttgacaca cattcaaagg ctcaccggct gctatctcca
     8881 cctgtttttt tactaaatcg attcctgtga ccatttctgt tactggatgt tccacttgga
     8941 ttcgagtatt catttccatg aaataaaaat cgccttcttc atccatcaga aactcgatcg
     9001 ttccggcatt ttcataattg actgctttag ctgctcgaac agcagcgtct cctagtgctt
     9061 ctctttttgt ttgtgagatc actacagaag gagattcttc cagtactttt tgattgttgc
     9121 gttgtaaaga acaatctcgc tcgcctaaat gaatgacatg accaaatcga tcacctaaga
     9181 tctgaacttc tatatggcga gcaggataaa tgattttttc caaatacata tcatcatttc
     9241 caaatgccgc tgctgcttct tgctgagctg aagaaaaatg ctgtgggagc tcttcttttg
     9301 attgaacttt ccgaatgcct ttcccgccac cacctgcagc tgctttcagc atcactggat
     9361 aaccaattct gtctgctatt tccaatgctt cttctactgt agataataca ccagtacttc
     9421 ctggaatgac tgggacattt gcttcttgca tcaattgacg cgcattgatc ttatttccca
     9481 ttgcatcgat cgttgcactt ttaggaccaa taaatgtgat attgcactct tcacacatgg
     9541 aggcaaatcg gctattttca gataaaaaac caaatcctgg atgaattgct tcggcttttg
     9601 tcacgattgc agcgctcagt acttcctgaa cattcagata tgaatcaacg gcttttgccg
     9661 ggccaatgca gattgcttca tctgctaact gtgtatgaag tgcttcggca tctgcttctg
     9721 aatatatcgc tacggtttgg atccctagtt cgcggcatgc acgaatgata cgtacagcga
     9781 tttcaccgcg gttagctatc aatactttag aaaacatatt tcacctatcc aatcataaag
     9841 gttaattccg cttcggctac ttttttaccg tctactttag cgattccttt gcctattccg
     9901 gctgaagctt tcactttcaa tatctcaact tcaaggtata aggtatctcc cggtgtaact
     9961 ttcttacgga attttgcttt gtccaatccg ccgaaataag ccgtctttcc cttgaattgt
    10021 tcaagagaaa gtaatgcaac tgctcctgct tgagccattg cttcaacaat caatacccct
    10081 ggcataacag gttcttctgg aaaatgtcct tgaaaaaagg gctcattgat cgttacattt
    10141 ttcttagcaa cgatccgttc tccttcaacc atttcttcca ctcgatcaat caaaagcatt
    10201 ggataacgat gtggaatgat ttcttttatt tcttgtatgt tcattgtttt ccctctttcc
    10261 tgctttgctt atttttgctg aatcctaaag agtggctgtc catattcgac aactgactcg
    10321 ttttctacaa ggatttctgt gatcactccg tcggcctcac tagttatctc attcatcagt
    10381 ttcatcgctt caataatgca gataacttct cctttggcaa cttgatcacc tacttttttg
    10441 aaagctggtt tgtcgggcgc tggttgcaga taaacgattc ccacgattgg cgaaacaacc
    10501 tcgattccat caaatactgc cgattctttt tctttcatta tttctggagc agttgttgct
    10561 tgttcaggta tgacattgac tggctccgaa actggcaacg tttcgtttgt ttgaaccata
    10621 ttcatgcctc gacttgtttt atttttattc atatacaatt caaattgacc ttcacgcaaa
    10681 tcgaattccg ttagcgagga ttgatcaaat tgactgacta attctctgat ttcattgata
    10741 tccaaaatta ttcctcccag cgtttcatgc acagaaccgc attatgtccg ccaaacccta
    10801 atgaattact cattgcataa gtaaaacttg caggtttgct ttcgtttttg acaactgtca
    10861 agtcaatctc aggatcttgt ttttctacat tgatcgtagg tgggataaat tgatgttcta
    10921 atgccagcaa agttgccaag gcttcgatcc cgccggctgc tcccaataaa tgtccagtca
    10981 tcgatttcgt gctgctgacg taagtatctc tgaaattttc acctaatgcg atttggattg
    11041 cttttgcttc tgcctggtca ttggctggtg tacttgttcc gtgagcattg atatatccga
    11101 cttgttccgg ctgtacacca gcttcttcca tcgcaagctt cattgcttta gatgcaccag
    11161 aaccatctgg agtaggagct gtcatatggt aagcgtcaca gtttgcaccg tatccaacga
    11221 tttctccata gattttggca ccgcgagctt gtgcatgttc taaagattca agaactagaa
    11281 cgcctgctcc ttcacccatc acaaaaccat tccgattttc gtcaaacgga atcgaaccac
    11341 gattaggatc agttgattct gagagtgctg ttaaagctgc aaatccagca atccccatct
    11401 cacaaatcgt tgcttctgtt cctccagcaa gaatgacatc agaatagcca tgtttgatgt
    11461 tacggaatgc ttccccaatt gaattcgttc cgctagcaca agctgttacg atagctgtac
    11521 aaataccttt tgcacctaca cgcaaagcaa tatttccagc tgccatgttg gcaatcgcca
    11581 ttggtacgaa cattggagaa actcgttttg cacctttttc atgtagacga ataacttggt
    11641 tttcgattgt tggaagacca ccgatcccag atccgatgat gacgccaaac cggtcttgat
    11701 tcattttttc tttatctaaa ccgctcatct ccattgcttc taaagcagcg tagattccat
    11761 agatcgaaaa catatccatt cgtttgctat cttttttaat aaaatattta tcgtaaggga
    11821 aatctttgac ttcacccgct aaggtaatcc ctgtttctga agcatcaaat ttagtgatcg
    11881 gtgcgatgcc attcgtcccc tcttttaagc tattcaaaaa gctgtctgcg tcatttccaa
    11941 taggtgaagt gactccatag ccagtaatta ccactcgatt catttgtttg ctctcctttt
    12001 cttatccgtg catgaccaat ccgccatcga cattgatgac ttgtccggtg atatatggat
    12061 tttgtgctaa gaaaactgct gtttgtgcaa tatcttctac ttgtccgaaa cgctgcatcg
    12121 gaatttgctt ttccgcttgt tccttgactt tgtctgctaa aacttccgtc atatcagttg
    12181 tgatgaatcc tggcgcaatg gcattacatg taatgcctct agttgcagct tctcgtgcca
    12241 cagattttgt taatcctact acgccagctt tactagctgc ataatttgct tgaccaatat
    12301 ttccaatcaa cccagataca ctagagagat taatgatggc cccttctcgc tgcttcatca
    12361 tttttttcaa tacgtgctgc gtcatattga aggttccaat caaattgata tccagacatt
    12421 ttttgaaatc ttctgcatcc attcgcatca caagtttgtc atttgtgatt ccggcattgt
    12481 ttactaacac atggatcgat ccaagctttt cttctgcttc tttgatcatt tgtcccgctt
    12541 tctcataatc agagatgtca ccggaaactc ctacgcattt tacaccgaat gcttcgattt
    12601 cttctatctt ttcttttgga atctctccgc gaccattcaa aatgatattt gctccagctt
    12661 ttgcaaaagc taaggccatc gctttgccga ttcctcttgt actacctgtg ataaatacat
    12721 tttttccttt tacatccata acgttcctcc ttatagtatt gctattgttt catcaaatgt
    12781 ctgcttattc tcaacacgca gaactggtat tgttttatcg atttttttca taaatccgct
    12841 taatactttt ccggggccga cttcgatgac ttgttcgatg ccgattgttt ttaacttatg
    12901 gatactctca tagaaacgaa caggttgcat gacttgctgt tcaagtagtc ccgcgattgt
    12961 ttctttcttc attatttccg ttgttgtgtt gcttatgata ggaaaagaag gttcagagaa
    13021 ttgaatctga cttaggtcct tcgccagttt ttttgctgcc ggttctaaaa tagctgtatg
    13081 aaaaggcccg ctgacattta gagggatcat tcttttgaac cctttttctt ttaatagtaa
    13141 aacagcttcg tctactgctt tttcttcgcc accaatcacg atctgctgag gtgtattata
    13201 atttgcagga gatacgatgc cgtacttgct agcttcatga cagctttcct cgatcgtttc
    13261 aatcggcgca ttcatcactg caaccatttt cccgcttcca gctggggcag cttctgtcat
    13321 ataagcgcct ctttttgcta ctaacgcaac tgcttcagtg aaggatagcg cattactagc
    13381 tactaatgcc gaatattctc ctaaactcag tcctaaagct gcatctggga tgattccatg
    13441 ctccttcaat aagcgataat acgcgatgct gactgttaga atagctggct gtgtatattg
    13501 cgtctgatcc aaacgttcat tttctgtaaa gcaaagttca gccatatcgt agccgagaat
    13561 ctcacttgct tcatcaaatg tctgctttac aacggcttct tcatataaat cttttcccat
    13621 accttgatat tgtgcacctt ggccactaaa taagaatgct gttttcatgt ttttctccaa
    13681 tatatccaga atatatgttt gattcttcct attcaaaaag gccgagacat tcctatttca
    13741 ggaaatgtcc cagcccccaa cctaatttta atgattatgc agtttgtttt tctacgtaag
    13801 tcactaaatc ttgaaccgtt ttgattccgt cttctgtttc gattttcacg tcaaattcat
    13861 cttcaatttc attgatgatt tggaataaat ccaagctgtc agcttctaag tcttcttgga
    13921 tgtcagtagt caatttgatt tcttcagttt cttttcctaa ttcttctgcg atgatttctt
    13981 gaattttttc gaataccata ataatatatt cctccgatat tgtttgcttt ttatttttat
    14041 aatttaacag tctacaactc catgagaata cttccccatg ttaaacctcc gccatagcct
    14101 gtaaatatta ctttctgctg ttttcctaag gtgatctttc cactagaaac tgcttcatcc
    14161 aataagatag ggatgcttgc agcagatgta ttcccatatt tatccatatt ggtcaggaac
    14221 ttttcttgcg gcatctttac ttttcgggca attttctcaa taattctgag gtttgcctga
    14281 tgcaaaagca gataatcaac tggtttgtct ttcatgactt cttggatatt gtttgcaacg
    14341 tctcgtacgg caaaatcaaa aatatctctg ccagtcattt gcaaataata cgaaccttct
    14401 gaatcttctg aatagaacgg atttttattt tgctggtagc cagcggtcaa tgagctactt
    14461 ctggtaccgt cagctgctaa tttttctttc agcaattttt tctgtggatt cgcttctaag
    14521 agaatcccac ctgctccatc gccaaacaac acagcagtcg tacgatcaga ccaatcgatt
    14581 agcttagata gtgtttcacc accaatcacc agtcccttag ttgagccgca gcggatcatt
    14641 ttttcagcca tgcttaacgc ataaacaaat cctgagcacg cagcactgat atcaaaagcg
    14701 atagcatgag ttgctcccaa tttcccttgt acttgtgctg ctacagatgg tgtcgtgtaa
    14761 tcgggcgaca ttgttgctac taaaataaaa tccaaatctt tagggtttag atttttctgt
    14821 tttatcaatt tttccgccac taaaatgcat aaatcagaag tctcctgatt tgttacacat
    14881 cttcgttcac gaatacccgt ccttgacgaa atccattcat cagaggtatc catcatcttg
    14941 gaaagatcat tattagtaat aatcttttca ggaaggacgc tggctgttgc agtaatcatt
    15001 ccaaattgtt ccattaaata tcctcactta tagtcttgca agaagtcgtg caagtttttc
    15061 agtgcatcca gcaaggcatg ctgttcctcc gtagccattc cgtctaagat gttcttgacc
    15121 atttcacgat gaaaatgctg atggacccga aatagtaact ttcctttttt cgttagtccc
    15181 aactttacaa cgcggcggtc atcttcactg cggatacgtt cgacatagcc tttttttact
    15241 aacttgttaa tagcagtggt caaggtccca acagtaattg atagttcttt ggctacttct
    15301 gaagttgttt ttttcttgta catcccgatt gcctcgatcg tgtgcatctc tgtgattgac
    15361 agatctttga attgagattt tttcagctca gactcttcga ttgtcaaaat atcattgaag
    15421 acactaacaa gataatcatt tactgttttt aaatctggtt ccatgttcca ccgtccacta
    15481 attagtttga ttatcaaatc ttttgatgtt caaactatat cttaactttt ttcaaaatgc
    15541 aaccttaaaa gatcattttt ttactatatg agcttttttt aagattttac tcatttaaaa
    15601 caactgataa atcagcattt ataatttctt ttattagaaa aatcatctat ctcacatatt
    15661 ttgattatca aagtattttc tgtgaacaga actatagaat tccctaaaac ttgtccatta
    15721 tttcaaaatt tgttatagtc tttttattca aagcgtaaaa ggagatccta tgttaaaaga
    15781 aaaaatgaaa actctcccca tgctgccggg tgtctatctt atgaaagacc gagatggtaa
    15841 ggtcatctat gttggtaaag caaaacggct caaaaataga gtaagcagct attttcatca
    15901 gaataaacag cattcaaaaa aagtattacg tatgattcat catataacag attttgattt
    15961 cgtcgtagta gacacggaat tagatgcttt attgctcgag tgtcaattga tccaacatta
    16021 ccgtcctttt tataaccgac aaatgaatta tttcagtaat tataattatg tacatattac
    16081 caataaggga tttgtattaa cagatactcc tacggctcga acatacgggc cttttcgtct
    16141 ctataaaaaa atgccgtcta ttttgcgtat aatggaagaa acttatcaaa tgccttggtt
    16201 gtctgaaatt agtttgttag ctttgcgagt ccagcttcct gacctacagg aaatgacttt
    16261 tgaacagaaa aaaaaggaac tccaagggct gtttcaaggc cgaaataaaa agctactgac
    16321 ttacttgaaa aaaagacaac aacatttcat ctatcagttg aatttcgaaa aagccggtat
    16381 gcttcaaaaa gatatcgaat tagttactta ttttattaga cggatccagg aacaaaagca
    16441 gtttttaaga acaccatcgc tcaccttcag catgccatta gcagcagatg agagtcagaa
    16501 aaagcactac ctcgtttgtt atggtcaact tgcagaaaca atgattacct ctggtgacgt
    16561 ttctcctgac ttctattatg aaaagaaaga agctcattta tctttgaaac gtcaattgtc
    16621 aaaagaagaa atcgatcctg tacaaatctt gatcagttac cgcaaaaaat tagaaaaaga
    16681 acagtttgaa attgaactgt taaacaaaaa agaagctgaa aagcaactaa attagttgct
    16741 tctcggcttc ttttaggcat cttcaacttc aaatgtcaat tatcctaaaa tttattggat
    16801 taagtcatag atttccatcg ctacgatatc gatattatca aattcgtatt gctgatgagt
    16861 atctgtttca gttaattcaa atgtatcggt cgctttatca taagttactg tacacttctc
    16921 tacgccttct ttttcaaaac ggcgaacttg cagttcatta tctgctgact cgaccatagc
    16981 ctccagtcgt tttatgattg cgactaattg tgaatgtgtc atcggaggtc ctccttttct
    17041 tatttctgtt atattgtatc aaaagcatag cctacttgca ttgattttct ctaaaaaaac
    17101 tcgtttttta tgattttttt tctggtattt ttccttcagg ccaccaaagt ttgatcaaag
    17161 cttgggttcc gtcattctca aaacctttat ctgccaactc ttcatatagt ttcaatgctt
    17221 gggtagttgc tggcaataca agatccattt tttctgcttc ttctaatgca atctttaaat
    17281 ctttgataaa atgtttgaca aaaaaaccag gtgtgtaatc ttcttttaaa atacgaggag
    17341 aataattgct taaagaccag ttagctgcac ttccacctga aagcgtctca atcactttct
    17401 ttaaatcaag tccgtttttt tgtgcataga caagcatttc cgtcattcct gtcatcgtac
    17461 ctgcaatcat gatctgatta gccatttttg tatgctgacc tttcccagca gaaccatgca
    17521 atgtaaatgt cgtaccaaat tctttgaata aaggcagtac ttgatcataa acggcttctt
    17581 ctccaccgac catgatcgtc agtgtaccat tctttgctcc taagtcacca ccggataccg
    17641 gtgcatctaa cgcatctgcg cctttttctt ttgctgtttt tgcaattttt tctgccaggc
    17701 ttggtgtatt tgtagtcaaa tcaactaaaa tatgtcctga gatatctgca gaaaaaatcc
    17761 ccgactgtcc atagtagatt tcttctacat ctttggggta acctaccatt gtaaagagaa
    17821 tattactttg ttctgctacc gcttttggtg tatctgccca gatagctcct tctgttacta
    17881 aaggatcagc ttttgctttt gtccgattat agacagctac ttcgtgtcct gcttctagta
    17941 gatggcgtgc gacagcactt cccatgacgc ctgttccgat aaaaccaagt ttcatttttt
    18001 cgacctccta aaaatcaaaa atccttgctt ctatgctagt atacgctaga aacaaggatt
    18061 ttttcataaa aaatgtttga attttatctt ttaaatgaat ttaattttat tttgattcgt
    18121 tcaatcgagt cttcttcttc ttttcttcgc atctcttctt catgataaac aaccatgtct
    18181 tgataaaatt cgatcatagc ttcgccgaaa tgtgtagatg aaatatcata aagtttagct
    18241 tctaaaactt ttggatcttt aggaacttca ctggaaatat aatcaataag tgctggtgca
    18301 aagtcttcgt cggtttcaaa agttttccct aatgaaggat gatcgaacaa tcggttcaaa
    18361 taagcatttc cttccacgac acaaggtgtt ccggctgcca tcgcttctgt ataagtaagc
    18421 ccttgtgttt ctgaagtaga agcactgaca aaataatctg ccgctttata ataaagtgct
    18481 acatcattgt tgtccacttc tcctgtgaac tgcacaaatt cttctaactg cagttctgag
    18541 atcaattcct ctaactcttc tacataaggt ccttttccta cgataactaa tctggcgtta
    18601 ggataagcgg agatgacctg aggaaaccca ttaatcaagg cttggatatt tttctcataa
    18661 gaaatcctgc ttaaagaaag aatcatcaaa tggttttcct gaagacctag tgactccctt
    18721 aatttgctga tatcctgctg agtgatatca gaacgtttga atttttcaat gtcgatacca
    18781 gtaggaatca cgcgaagcgg tgctattaca ccatagctgc gtaatgtatc aataacacgt
    18841 tcacttggac aaactacacc tgtcgtatga tttgcaaata atctagacaa gtattttaca
    18901 tgggattgcc tgatcacttt tcctttaccg atataatgaa ggtagtcttc atacatcgta
    18961 tggtaagtat ggatcagagg tattttcaac ttcttagcta ccatcttccc aagtatccct
    19021 gctccgaatt ctgtatgggt atgaataaga tctagttcta gttctttggc aaccaaataa
    19081 gcataccaca tgcctctaac aacaactctt cgatccttga aagaaataaa tggtacactt
    19141 ggcattcgaa tcacatcttt ttccaactta tccgcattag gatcggtcgt tgtaaaaata
    19201 tagacttcat gcccatgtct ttctaattct tgttttaatg ttttgatcga tgtagcaact
    19261 ccgctgactt gcggaaagta ggtatcggta aaaaagccga ttttcatgtt tttcgcctcc
    19321 aaactagtcc ttgacctgat ttactttttt taatttgcta gagacttttt cattcagtac
    19381 actttcgtat acatttttaa gttcttcacc gattttattg atacttcgct cttttgctgt
    19441 ttgataaccg gcttgtgtcg tactcggtag ttcttgattg gtaatctgtt ggatcagtcg
    19501 gataaattct tcgtttgtct tccccatata gcagttcttg ctatcttcta accagccatt
    19561 atacactgga atatctcgaa caagtacgtt ttgtttgctt gctaaagctt ctaaaacaac
    19621 gatcccttct gtctcttcat aagaagggaa aaagaataga tctgcacctg aatacgcacc
    19681 ttctatgatc tctccacgaa tgtaaccagg aaacaggaca ttttcaggat gatcttcttt
    19741 tacaattttc cggatgttcc gaggaatcga gtacatggga acatgaccaa accaaataaa
    19801 tgtgtattcg ggcatcttct tagcaatttc tacgaaatca atgattcctt tacgttcgaa
    19861 aaacaaaccg acacaaatga ttactttttg atccggagac agtttgaaat attctctgaa
    19921 cttttgttct ttcattggat cagggtgata tttctctaag tcgatcccat tggaaatagc
    19981 ttgaatcggc acacggatac catagctttc caacaactgt tttgaataag gtgttggcgt
    20041 gatcagatag tccgcttttt ggtacaatcc taccagatat ttttttacga taggagaaag
    20101 ctggttcgat ccgataaagg aatttcggaa atcttcctct gtagaatgcg catgatagac
    20161 aacttttttt ccttctcttc tggctttatt gaccatatta tgactattga ttccgtatgt
    20221 attgatgtgc aggatgtcat aatcttcttt ttcatctagt gtataagaga taccgacttc
    20281 cgtcagagca cgtttttgat gatcaagcgc acgcccgatt ccagattttg caagcatctt
    20341 ttcactttca aaatataata ataccttcac cttacgtccc ccttcactta tatagtataa
    20401 catagcctac aaactgtcac tcaaacttaa gccctatttt tgtctattct tactgacaaa
    20461 aacgttttct taaaaagaac aaaaaaactg gcgtagctct tttacaagcc acaccagctt
    20521 ttgatacgtt gtttatttta catattcatg aacaagttca acgacttctt ccatcatatc
    20581 gcattctttc aatgcacgat cagcaagttc agccattttc gctgtgtcca agcgtttcat
    20641 taaactacgc gttttaagaa tagatgtcgc actcattgag aactcatcta gacccatacc
    20701 tactagcaat ggaacagctg tttgatctcc agccatttct ccacacatac ctgcccattt
    20761 tccttcagcg tgtgctgcat caattacatt tttgatcaaa cgtaaaattg atgggttata
    20821 tggttggtac aagtaagaaa cgcgttcgtt catacggtct gctgccattg tgtattgaat
    20881 caaatcgttc gtacctacag agaagaaatc tacttctttt gcaaacttgt ctgctaagac
    20941 tgctgctgct ggaatctcga tcatgatgcc gacttggatc gtatcagata cttccttgcc
    21001 ttcacttatc aatttttgtt tttcttcttc aaagatcgct tttgctgcgc ggaattcttt
    21061 gagtgtagct accataggga acatgatacg taaattacca tgaacagatg cacgaagcaa
    21121 tgcacgcatt tgtgtacgga acatttcgtc gccttgttca gataaactga tacgcaatgc
    21181 acgatatcct aagaatggat tcatttcatg aggtaattgc agatatggta gttctttatc
    21241 tccaccgatg tccattgtac gaacaactac aggttttcct tccatacctt ctaatacagc
    21301 tttgtaagct tcatattggt catcttctgt tgggaagtct ggagaatcca tatacaggaa
    21361 ttcagtacgg tacagaccaa ctgcttctcc accattatta tggacaccaa ctagatcttt
    21421 tggtgttccg atgtttgcag ctaattcaaa atgcttgccg tcagctgtga cagtttctgc
    21481 attttttaat ttttcccatt ctgctttttg tgctgcgtaa tctgcacctg ctttttcaaa
    21541 ttcagctttt tgttcatctg taggatcgat gattacatcg ccttcgatcc cgttgacagc
    21601 caaaatatcg ccttctttga ctttagcagt aatttctttt gtcccaacga ttgctggaat
    21661 ttctaaagaa cgtgccatga tagcagaatg tgaagtacgt ccaccaatgt ctgttacaaa
    21721 cgcttttaca aaattgcgat ctaattgtgc tgtatcactt ggagtcaagt catgtgcaac
    21781 aacgaccact tcttcgttga tcattgatgg atttggtaaa gtaacgccta ataagtgcgc
    21841 taaaatacgt ttagcaacgt cacgaatatc tgcagcacgc tcttgcatat aagcattatc
    21901 ttccattgct tcgaacatac cgatatacat atccgttact tcttttagcg ctgattcagc
    21961 atttacgctg ttgtctttaa tattttgctt gatttgacct accatttctg gatctgaaag
    22021 aaccatcaaa tgggcatcaa atacttgcgc ttctgcttca cctaagcttt gcgctgcttt
    22081 ttcacgaatt tgctgcaatt cttcagtaga tttagctaat gcagcatcca aacgagcttc
    22141 ttcagccgca gtatcttcta ctgttgcctt actgaatgtt aaatccggtt gaactagcag
    22201 gtaagctttt gcaacggcta caccgtcact agcggcaatc ccttttagca tttcaaccat
    22261 tattcagata agccttcttt cttcattgtt tcaacgattg ctgccattgc atcagcttcg
    22321 tcagcacctt ctgcagtaat agtaacatca gaaccttgac ctacacctaa agacataacg
    22381 cccatgattg atttcaagtt tactgattta cctttatact ctaagtttac atcagagtta
    22441 aatttgcttg ctgtttgtac taatagtgta gctggacgag cgtggatccc agtttctgct
    22501 actacgtgaa attctttctt ttccatatta atcggtctcc tttaagtgag tgttgttttt
    22561 atgtgagggt tttctttttt agtaattcag agaatcctaa caaatgccac cctttcatct
    22621 gtaaagatta acattttttt tcgttagata caacagtttt cattcaattt cagataaaag
    22681 tattcaaatt attccggatt ctttttcatc cttttcttca cattttataa caaacgtgtt
    22741 tatttccttt ttcttgtcgc tttctcttct cctgacgttt atcattcttt cctttgaccc
    22801 tctttgtttt catatgaaac gaattacttg cttccctttt tatttttagt ataataagag
    22861 tcaaaggtca ggaatggtca atagagattt caccttttct acctcataaa tctcttaaaa
    22921 aactaggggt tattctttta cctcatgaaa aacgagatgt aaaatatgga tgtacccatt
    22981 tttgtgaaag gacgtgaatg attcatgctt tgtcaaaact gcggcaagaa tgaggcaacg
    23041 attcacttgt atgccactgt caatggtcag cggacacaac tcgattactg tcagagctgt
    23101 tatcaaaaaa tcaaaaatca acaaaatgga ggtctgacta atatggccca aaatgatcca
    23161 tttggatttg gcagtttgga cgatcttttc cgttccatgt ctcgccaaat gcaacagcaa
    23221 ggaaattatg agcaaacacc accaacacaa tttggcggaa acaacaactt caatggtggt
    23281 cagccacctc aaggcaatac agaaggttta ttgggagaat acggaatcaa tatcacagaa
    23341 caagcacgac aaggtgatat cgatcctgtg atcggacgcg atgacgaaat caaacgagtc
    23401 attgaaattt taaatcgccg aaccaagaac aatcctgtct tgattggaga accaggtgtt
    23461 ggtaaaacag cagttgttga aggtttagca caaaaaatcg tagatggtga tgtaccgcaa
    23521 aaattaatgg acaaagaagt cattcgttta gatgtcgtat ctttagtcca aggtactggt
    23581 atacgaggac aatttgaaga acgtatgcaa aaattgattg aagagatccg tcaagcagaa
    23641 aatgtgatcc tattcataga tgaagttcac gaaatcgtcg gtgctggttc tgctggtgat
    23701 ggaaatatgg atgctggtaa tattttaaaa ccagctttag ctcgaggaga attgcaaatg
    23761 gttggtgcta ctaccttaaa tgagtaccgc atcatcgaaa aagacgctgc tttagaacgt
    23821 cgtatgcaac ctgtacgggt agatgaacca actgtcgaag aaacgatcag tattttgaaa
    23881 ggtttgcaaa aacgttatga agactatcat catgtaaaat atactgatga ggccatcgaa
    23941 gcagccgcaa ctttatccaa tcgttacgtc caagatcgct tcttgccgga taaagccatc
    24001 gatttgctag atgaaacagg ttctaagaaa aacttgacga tccaaatcgt tgatcctaaa
    24061 acaatcgaga aaaaactgca agaagcagaa gaacaaaaag ttttagcttc tcgagaagaa
    24121 gatttcgaaa aagcagctta ttatcgcgat cagatcaata aacttcaaaa aatgaaagaa
    24181 cgtcagctca ctgaagagga aacacctgtg attaccgaaa aagatatgga aaaaatcgtt
    24241 gaacaacgga caggtattcc agtgggtgaa ttaaaagaaa aagaacagac acaactgaaa
    24301 aacttagcag atgacttgaa agctcatgtc atcggacaag ataacgctgt agatcgagta
    24361 gcgaaagcga ttcgccgaaa ccgtgttgga ttaaacaaac agaatcgtcc aatcggctcc
    24421 ttcttatttg ttggaccaac tggtgtaggt aaaacagagc ttgcgaaaca acttgcctac
    24481 gaactattcg gttcacaaga ttcaatgatt cgtttcgata tgtctgaata tatggaaaaa
    24541 catagtgtct ctaaattgat tggttctcct ccaggttatg tcggatacga agaagctggt
    24601 caattaacag aaaaagtacg gagaaatcct tacagtcttg tcttgttaga tgaagtagaa
    24661 aaagctcatc cagatgtttt gcatatgttc ttgcaaatct tagatgatgg tcgattgaca
    24721 gatgcacaag gaagaacggt cagctttaaa gatacgatca tcattatgac aagtaatgcc
    24781 ggaactggta aagtagaagc aaacgttggt tttggtgcgg cacgcgaagg tgttactcgt
    24841 tctgtattga accaattaaa caactacttt acaccagaat ttctgaaccg ttttgacggg
    24901 atcatcgaat tcagtgcatt atcaaaagaa aacttgatga caatcgtcac actaatgcta
    24961 gacgatgtca accaaatgct ggcagcacag caacttcaca tcgaagttcc tacgaatgtc
    25021 aaagaaaaat tagttgactt aggctatgat ccatctatgg gtgctcgtcc tcttcgacgc
    25081 acgatccaag aacaaatcga agatggtatc gctgaatttt atcttgatca cccttctatc
    25141 catgagttga aagcaaaact ggataaagat ggaaaaatca tcgttacttc taaaccagaa
    25201 cgtttggcta aagaatcagc cgaagaaacg gctgaataaa tccagtttta tagcgtgaga
    25261 cggtatttct ttgagaaatg ccgtctcatt cttttttcag aaaagtcaaa atactgttca
    25321 ttttataaaa aagaaattat aataagagaa acaagcaatt aaaaatacat aaaagaggtg
    25381 agcgccatga aattggtaaa tgtaacaaac agccattccc gtcttgtatt aaaccagctt
    25441 gaaaacactg atgcccatct agtcaaggtc tacacagccg gaaataccac tattgtctac
    25501 actgaagccc ctgaacataa tgaaattctg ctgatcaatg acaaacgtaa aatccagccg
    25561 aaagaaatcg aagatgctaa aaattatttc ttagataaaa tcaaagacgc cgtttaccac
    25621 gaggatgaaa tcaaagtaat cgaattggca ggccttgttg aaatctcgat tccaaaacat
    25681 gaattaggcg ctacattagc cgtgtaaaca aattttgaac ataataatta aagagcgtgg
    25741 gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg cgaaagcaga
    25801 agcaactcct tcggaaataa gctgaaattc acgaaaattg cttttcaaat ttttgtgaat
    25861 tccagcttat ttcttggagt taaacgcttc tgtctcagcc tctttttctt tctagctttt
    25921 tgatttttct cttttctttt gtacttttct cttttcattt tcagttctac gcgtgtcttt
    25981 atttttaact ttttcctctt tatggatgat tgatcgtact ttttgtgcaa tttgctcgtt
    26041 tttgtcccag tcttttacat ctaataatcg acataatcgt aacatctctt ctttttctgt
    26101 ttttttcttt cacgtctttg ccattctgtt tccatgtgtc acactctcct ttctttaatc
    26161 atatgtacgc aaaataattt ttatattttt ttgattagac atttctatga ttttaatata
    26221 gtaaaaagag ccgtcacgga aaatccgtca cggctctaaa caaaatatga gaggctgttt
    26281 ttgtttggct attcttatta cttgatgcag ggcagcttgt gtcaagactt ctttacatca
    26341 aactttttaa ttttacattg ggatatttat ccgcaaacca gcgttctgca aattggttct
    26401 cgaataagaa caacggttgg tcgaaacggt cacgagccaa gatattgcga cttgaactca
    26461 tcttctcatc caaatcttct ggatcgatcc agcgagcgat tttgtgtccc attggagaca
    26521 tgattacttc ggcgttatac tcatttaaca tacgatattg gaatacttcg aattgtagct
    26581 gaccaacagc tccaatgata tattcttccg ttagatatgt tttataaagc tgaatcgctc
    26641 cttcttgaac taattgatag attcctttgt ggaaggattt ttgtttcatg acattttttg
    26701 cagtcacttt cataaacagc tctggagtaa atgaagggag ttcttcataa gcaactttta
    26761 gttttccttc gaacaatgta tccccaatct gataattgcc tgtatcatat acaccgatga
    26821 tatcgcctgc tactgctttt tcgacgtttt ctctcgaatc tgccataaat tgtgtgacat
    26881 tgcttaattt cagtttttta tttgtacgtt ccaaccagac atccattcct cgttcgaatg
    26941 ttccagaaca gattcggaca aaagcgattc ggtcacgatg ggctggattc atatttgctt
    27001 ggatcttgaa tacgaaacct gaaaattctt cctcataagg acttaccatc tgatcatctt
    27061 gtgttttgtg tccataagga gctggtgcca attcaacgaa agtctctaaa aaagtctgta
    27121 caccgaagtt agtcaatgca gaaccaaaaa atactggtgt ctgatcgcca cgagcaattt
    27181 tttcttgatt aaatgcatct ccagcctctt tgattagttc tacttcttct aagacttgtt
    27241 catataagga attttcagtt agaggatgat tttcgggaag cagtccatct tcatccaatt
    27301 ctactaatcg ttcatcctga taattttccg gacgataaaa ctctacacga ttatggtaga
    27361 tatcataaag accttctagc cctttaccca taccgattgg ccaattcatc ggataagatt
    27421 cgatatctaa tagctcttct aattcttcta acaattccaa tggttcacga ccatcacgat
    27481 ctagtttgtt gataaaagta aatatcggga tgccacgttt ttttacaact tggaataatt
    27541 tctttgtctg tgcttcgatc ccttttgcac tatcgattac catgaccgca ctatccacgg
    27601 ccatcaaagt acgataagta tcttcagaaa aatcttcatg tcctggtgta tccaagatat
    27661 ttactcgttt cccttgataa tcaaactgca taacagaact agtaacagaa atcccacgtt
    27721 gtttctcgat ttccatccag tctgatttgg caaaattacc tgtttttttg ccttttacgg
    27781 tcccggcttg acggatagcc ccaccaaaca atagtaattg ttctgtaatc gttgttttcc
    27841 cagcatccgg atgggaaatg atcgcaaaag ttctgcgatt gtccacttgt tgctttaatt
    27901 ttggattatt cataacctaa ctccatttct tccattcaat tatttatatt ttctctaggc
    27961 acagattgtc cgatctctta aagaaagaca atttcatata gggctgtgac aaaagccgtg
    28021 tagcagtctc tttttccgag taaacggcgt tctagagaca gcctatcagc aataagccca
    28081 cccaagccaa aaatacgaaa agctgttttt gcttggctgc tcttattact taatgcagga
    28141 tagctttttc acaacctttg tcaactacta caactgaatc agtatagcat ggattcagcg
    28201 aatgaaaaag ccaccaaacc ataagagtca aatatttttt tgtcagctga ctaaaagaaa
    28261 agacgtgttg cttgtttatt caagcaacac gtctttcata accaagtttc caattatctt
    28321 cttggctcgt catcttcgaa ttcttttctg aagtatcttc gttcttcttc aggttccgct
    28381 tctgtttctt ccggatcatg aaaaatgaca cggattttaa ggattcgaga accttccatt
    28441 tcttcagaaa ccagcgtaat attatcgaca tcaaacgata acttttcgcc ttcatccgga
    28501 atcatgccta atgctgtaat caaataacca gccatcgtat ctacatcact catatgcaga
    28561 tcactgtcaa aagcttcatt gaactcatca atcagcatac gtccttggat gatatattcg
    28621 tgttcatcga ttttttcata aagattctca acttcatctg attcatcatc gatttctccg
    28681 acgatttctt ctaaaaggtc ttctagtgtt gctaacccaa cgacgccgcc atattcatct
    28741 aataaaatag ccatctgatt ctgcgtcttt ttcatttcat ataacagatc atcaataaag
    28801 accgtttcag gaacgaataa aggctcctgc atgattttct tgatatctaa attatcaaag
    28861 ccgaatttat gggcagcttt tagtaaattc tttgtatgaa ggattccgac tactttatct
    28921 ttgtcttcgt tataaacagg gattcttgaa tagttttcgg ataaaacttc attcacattt
    28981 tcttccacag tgtcgttgat atcaatcata aatgcatccg ttcgtgggac catgacttct
    29041 cgggcaactt tcgtatcaag agaaaaaacc ccttggagca tttctaattc ttcattattc
    29101 aatacgcctt cgttttcaag catataacgc atttcatctc tggtcatttt tgaatcttca
    29161 tcatcaaaag tcatcggcgt gatacgcgcc aataaacttg tggaagcaga aagcagccag
    29221 acgaagggct tagctactac accaatcaag cgtatcagcc ctgaagtgaa ctttgcgact
    29281 tcttctgatt tatttaaagc aatacgttta gggtacaatt ctccaaaaac aatcgataca
    29341 taggtcaaaa tagccagcac aatgatattt gcaatatttt ttgccgctgt gccgccacct
    29401 aataccggag ctaaccgttc cgataaagta ttagctagtg aagcaccaga caaaatgttg
    29461 accaaagtga ttcctacttg gattgtcgat aggaaattat ttggattctg caatattttt
    29521 aaaagctttt gagatttcac atctccttca tcagcttttt gctcgattcg attcttattg
    29581 atcgaaacaa cagcaatttc ggaagctgct aaaaaagcat tgatcaatgt caaaacgatc
    29641 aataataaaa tctgcgctaa cagcgactga ctctcagggt cagcattcat aatcgcattc
    29701 tctccttata aaattaattt ttagtttacg atagaaaaaa acgtggcact atagccacgt
    29761 taaaatggta tcacaaagca attttctttg caataaaaac aagtgtacta aaactaccta
    29821 cgaggtcgta gcaattttct tcttgttttc attttgcttg tcttctttga cgattttgat
    29881 tatataggaa atgatcgtct tgcagattgc gtaaaaagga acacctaaga agataccaag
    29941 caatccggca atatttccag ctactagaag aacgataatg atcgttaatg gatggatttt
    30001 cagtgatttc ccaattacat taggataaat gatattccca tccaactgtt ggacgattag
    30061 cacaaccaca caacaaagta atgcttttac cggatcattg aagactgtta ctagaaatgc
    30121 aggtgctagt cctagataag gtcctaaata tggaattagg tttgtcaatc ccgcaatgac
    30181 cccaaataag aaagcataat ccacaccaat taccatatat cctaaaaatg tgaaggtacc
    30241 aacgaataaa cattcgattg cttgtccgct aatgtaatta gccaatgttt tattcagttg
    30301 ccccaataac tccacaattt ggtttcttct tttttctggg aaaaactgtt ggatgtttgg
    30361 cactagtttt tctccgtctt tcaacatata gaacaagatg aatggtgctg taatgattac
    30421 tacggttgcc gaagcgatcg ttccaacgat cgaacctaaa ctattggata ctccactaag
    30481 gaattgctga atgattgtcc cgtaagaaat gtctaattgc tcaaaatatt tattgatatc
    30541 tacttccttg aacaaaggca attcggctac ctcttttaac cagctttcta cctgtttgat
    30601 gaaatcaggc atattagatg ccaatgagga aatttgctgt actagacttg ggatgacact
    30661 aagtaaaatc caaataattg ctgcaactaa aaggagcagc acaattgcga cagcccatat
    30721 tcttttgatt tttgctttca tcaacagctt gacaatagga ttcaataaat agtaaagaaa
    30781 gcctgcaatc agtacaggtg caaataacgt ggaaaaaaat gtcccgatcg gttcgaaaat
    30841 aaagtttatt tctgatgaaa tcatgatcaa tgcagctatg atcaataatt caagcgacca
    30901 aaacattagt ttagactgtc taagtttttc aaacaataga tttcctcct
//
LOCUS       DACECZ010000019        30744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.019, whole genome shotgun sequence.
ACCESSION   DACECZ010000019 DACECZ010000000
VERSION     DACECZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.019"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            37..735
                     /locus_tag="IWO35_000859"
     CDS             37..735
                     /locus_tag="IWO35_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0315934.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(903..3542)
                     /locus_tag="IWO35_000860"
     CDS             complement(903..3542)
                     /locus_tag="IWO35_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0315935.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(4161..5192)
                     /gene="queA"
                     /locus_tag="IWO35_000861"
     CDS             complement(4161..5192)
                     /gene="queA"
                     /locus_tag="IWO35_000861"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ0315936.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            5385..6560
                     /locus_tag="IWO35_000862"
     CDS             5385..6560
                     /locus_tag="IWO35_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0315937.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            6564..7205
                     /locus_tag="IWO35_000863"
     CDS             6564..7205
                     /locus_tag="IWO35_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0315938.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            7202..8119
                     /locus_tag="IWO35_000864"
     CDS             7202..8119
                     /locus_tag="IWO35_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0315939.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            8116..8787
                     /locus_tag="IWO35_000865"
     CDS             8116..8787
                     /locus_tag="IWO35_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0315940.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(9328..9816)
                     /locus_tag="IWO35_000866"
     CDS             complement(9328..9816)
                     /locus_tag="IWO35_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ0315941.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(10017..10283)
                     /locus_tag="IWO35_000867"
     CDS             complement(10017..10283)
                     /locus_tag="IWO35_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0315942.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(10283..10546)
                     /locus_tag="IWO35_000868"
     CDS             complement(10283..10546)
                     /locus_tag="IWO35_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0315943.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(10539..10811)
                     /locus_tag="IWO35_000869"
     CDS             complement(10539..10811)
                     /locus_tag="IWO35_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315944.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            11022..11207
                     /locus_tag="IWO35_000870"
     CDS             11022..11207
                     /locus_tag="IWO35_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0315945.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(11349..13172)
                     /locus_tag="IWO35_000871"
     CDS             complement(11349..13172)
                     /locus_tag="IWO35_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ0315946.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(13770..16475)
                     /locus_tag="IWO35_000872"
     CDS             complement(13770..16475)
                     /locus_tag="IWO35_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ0315947.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(16781..17728)
                     /locus_tag="IWO35_000873"
     CDS             complement(16781..17728)
                     /locus_tag="IWO35_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ0315948.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            17918..18187
                     /gene="rpsN"
                     /locus_tag="IWO35_000874"
     CDS             17918..18187
                     /gene="rpsN"
                     /locus_tag="IWO35_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0315949.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(18363..18725)
                     /locus_tag="IWO35_000875"
     CDS             complement(18363..18725)
                     /locus_tag="IWO35_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0315950.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(18736..19857)
                     /locus_tag="IWO35_000876"
     CDS             complement(18736..19857)
                     /locus_tag="IWO35_000876"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ0315951.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(19873..20223)
                     /locus_tag="IWO35_000877"
     CDS             complement(19873..20223)
                     /locus_tag="IWO35_000877"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ0315952.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(20353..21864)
                     /locus_tag="IWO35_000878"
     CDS             complement(20353..21864)
                     /locus_tag="IWO35_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0315953.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(22186..23538)
                     /locus_tag="IWO35_000879"
     CDS             complement(22186..23538)
                     /locus_tag="IWO35_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ0315954.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(23691..24767)
                     /locus_tag="IWO35_000880"
     CDS             complement(23691..24767)
                     /locus_tag="IWO35_000880"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ0315955.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            24963..26708
                     /locus_tag="IWO35_000881"
     CDS             24963..26708
                     /locus_tag="IWO35_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ0315956.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(26988..27425)
                     /locus_tag="IWO35_000882"
     CDS             complement(26988..27425)
                     /locus_tag="IWO35_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ0315957.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(27409..28782)
                     /locus_tag="IWO35_000883"
     CDS             complement(27409..28782)
                     /locus_tag="IWO35_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315958.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(29104..29592)
                     /locus_tag="IWO35_000884"
     CDS             complement(29104..29592)
                     /locus_tag="IWO35_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0315959.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(29614..30216)
                     /locus_tag="IWO35_000885"
     CDS             complement(29614..30216)
                     /locus_tag="IWO35_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0315960.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            30315..>30744
                     /locus_tag="IWO35_000886"
     CDS             30315..>30744
                     /locus_tag="IWO35_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0315961.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT         8862 a         6529 c         5718 g         9635 t
ORIGIN      
        1 tatattggtg ttattaaaga aaaggaggga aaaatcatgc aatcttctgc agaattaaaa
       61 cgccaagcaa aagagagtct aaaaggacgt tggggacaag ctgttttact aaatctaatc
      121 cctacattga ttggtattgc tactattctt cttattatga ttcctattat cttgtttgcc
      181 accaccatat atgatggatc agcgagcgtc tcagaagcga caacttcagg aggcggtagt
      241 tcaggaggcg gtggcggaat tatttccagc atcattagcg ctttgttcat gagcggtatt
      301 tcttggactt atcttgatat tttacgtgga caaaaagatc agatcgaacc attcaaagat
      361 gcatttcgtg gattcaaagg tttgtttatc ggcggagtga tcagcttagc tgtgctgatc
      421 tcgatcttta caacattatg gggtttgcta ttagttattc ctggaatcat taaaagctat
      481 tcttattctc aaagctattt tatttactac gatatcgtcc aagaaacagg acaaacacct
      541 aaagtactgg atacgatcac tgcaagtcga aaattgatgg atggttataa aggacaactt
      601 ttctggttag atctttcatt tatcggctgg catatcttag cattagcaac tgctggtatt
      661 ggttatctat ggctcagccc atacatttct gcaacaaaag cagcatttta tgaacaattg
      721 ccaaaagata tttaaaagag agtgtgactt gtgaccgagg gggtggcagg agaacaccgt
      781 ttatcgagag gcagtaaaag aaacattcag cccattagca aaaaggaggt gtgacaacat
      841 tcatgtcaca cctccttttt gtatagttcc agtaaaaaga aaatcaactt agaaacagtt
      901 aatcattcct cttcttctgc ttccggtaat cgatggaact tgatcagttc attcaccgcg
      961 attggtacga tagataaaat ccctaccaag aaccagtgat tcaaactgat gtgtactagg
     1021 ccgaatagtt cttgcgtaaa tggcagcaaa gcaacagccg ttgtaatagc taatgccagt
     1081 aacgccattt ctaccaaagc tttattggtc gctaaattga ctttgaagat cgattggctg
     1141 ctgcgtacat tgaacacatg cagaatcgat gaataagcta agatcaagaa tgcaacagtt
     1201 tgaccgacat gctgacttgc gtctacccat gggctgatag tatcgacata actacctaaa
     1261 taataaccaa acaaggttac gatggtgaag acccctgcat ttatggcaat tttttgcaat
     1321 aaacctcgag caaagatccc ttcatttttc ggaatgggtg gctgatccat gatgccgtgt
     1381 tccgctggct ctcttcctaa agcaaatcct ggaagtccat cagcaactac attgacaaac
     1441 agtagttgca cagctgtaaa tggtgctccc catcctaaca gcatcgcaat caaaacaata
     1501 aagatctccg aaatattaca gcttagcaaa aagttgatag ctttacgaat attctgataa
     1561 accgaacgtc cttgactgac agcgtctacg atcgtcgcaa agttgtcatc cgtcaaaatc
     1621 atatctgcgg cactttgcgc cacatctgtt cctgtgatcc ccatggcaca cccaacgtta
     1681 cttgctttca acgctggtgc atcattcaca ccatccccag tcatcgctac gactgcaccc
     1741 gtacgttgcc aagatttgac gatccggatc ttgtcttctg gcgttacccg agcatagaca
     1801 gagagagctt tgacacgcgc atctaattct tcgtctgata attgatgcag ttctgatcct
     1861 gaaagtgctt ctgacggatc attcaagata cctaattctt tcgctatggc actggcagtc
     1921 accacatgat ctccagtgat catgactgtt ttgatgccag cctttttcgc tcgcttgatt
     1981 gctcctttac tttctggacg tggtggatcg atcattccga tcagaccaat caaacgtaag
     2041 tctttttcca gtgcttcaga agaaatatct tgcggttctt cgtcataagt agcataacca
     2101 acagcaatca ctctcagtgc ccgtttacca aagcgatcat taacgatcgc cgcttgttct
     2161 acatcgcctg aactaaagcg cggagcaaga acatcgaatg ctcctttggt gattgagatg
     2221 tatttttcgc ccatttggtg aactgttgtc atcatttttc gttcagaatc aaaaggaagc
     2281 tcgtttactc gtggatattt ttcttctaat tctgcctttg tatgataatt ctcttcaaca
     2341 gcccttacga tggctacttc tgttggatta cctttgataa caaggtcgtc atcttcttgc
     2401 tcgaccgtca catctgtaca tagtgcagcc attttcaata tttccatcgc gctgttcgtc
     2461 attgaatctt cgatattcgt tacttcatct tctttgctcc atacccggcg gacgcgcatt
     2521 tcattttgtg tcagcgttcc tgttttgtct gagcagataa cattagcggt tcctagcgtt
     2581 tcaactgctg gcaactgtcg gatgatcgca tgtttcctcg ccatcttctg cacaccgtat
     2641 gcaagtgtca acgtaacaat aacagtcaag gtttccggta cagctgcaac agccaaggaa
     2701 atcgaagtca tgaacatctc aaggagtggt tctccttgca gttcaccaat cacaaatact
     2761 aaagccgcag ccgctaaagc gatcatactg attcgtttgc ctagctgatt caatcggacc
     2821 tgtaaaggtg ttttttgcat agtattttcg ttcaaaagat ccgcgatctt acccatttct
     2881 gtagccatgc caatggcagt gacgatcgct ttcccacgac ctgcagcaac cgtacatcct
     2941 ttaaatacca tattaagttg gtcacctaat gactcatctt cttcagcaat aaatgctgca
     3001 tctttttcaa ctgcttcact ttctccagtc aacgccgatt cttctacacg taattgtgat
     3061 gcttcgatca aacgggcatc ggcagaaata gcatcaccgg actctaaaac aagaatatca
     3121 cccactacca gttgctctga ctcaacagtg gtcactttgc cgtctcgtat gacagttgcc
     3181 gtttgtttat tcatatcttg caaggattcc agtgcttttt ctgcgtttcc ttcttggaca
     3241 atcgccaata ccgagttgat cacaacgata gcgatgatca gcagtccttc aaataaatcc
     3301 ccatgttctg tagcaaatgc ggtataaaag gaaatcaccg ctgctacaag aaggatgatc
     3361 gttgtaaaat cggacaagct tcgaaagaat tttttgatca tcgattcttt cggcgcctca
     3421 tcgaatttat tcaatccctg cttttttcgc cgttcatcca cttcttgtga agacaaccca
     3481 gtatctggat ctgcttctgt taccttcttt acttcttcta ttgtctgctt gtattcttcc
     3541 atcctattcc tcctttaaaa tcttcgtaca aaaaaaggta cttattttac tataacatcc
     3601 tttttttatt tacttcaaat aaagggctta tcgattattt gttaagaaag cgtaaaattt
     3661 aaagtgctta tatttgcaaa cgctaccaaa ggatgctatt atatagatgt aaggaaggag
     3721 gataccatca aatagacaga agaatttctc gatagaacag tgtaagcaaa caaagttgtt
     3781 agaagtacag gtttcaccca gacatgcgtg tagagtacaa acctataaat agtgaatgcg
     3841 tatggatgtt tggatcacga agtatccgaa acaagaagta atgcgatacg taacaattga
     3901 ataacgtaaa cgtctgaaaa ttgaatagga acaagagatg tttcaaatgt tgaagatgta
     3961 acgaaaacaa aatttttgtt taacatgaag aaattcaaca ccgtgctttg catgtatgat
     4021 ggaaagttac aatcatttct gtttatttga atccttccga ggtacaagaa agccgagctt
     4081 atcgagagct cggctttttc tttgtcataa aatctttgta aagctttttt gttattcaaa
     4141 actagcggct tttcataacc ttatttcacg aacatcgcat ctccaaaact aaagaaacga
     4201 tagcgctctt caatcgcatg ctggtaagca gataaagtca attctcttcc tgcaaaggca
     4261 ctaactaaca tgactaaagt cgattttggc aaatggaaat tagttgagaa agcttctacc
     4321 actttgaatt catagccagg cgtaatgaaa atatcagtcc atccgctgtc tgctttgatt
     4381 tctccattaa atttcgttcc aatcgtttct aatgttcgaa ttgaagttgt tccgacagcc
     4441 acgattttcc cgccattttt acgtacttgg ttcaatcgct cagccgcttc ttccgtcaaa
     4501 cggtagaatt cactatgcat atggtgttct tcaattttat ctacgctgac aggtcggaat
     4561 gtacctaacc ctacatgtag tgtcagatag actaattcaa caccctttgc ttcgatctca
     4621 tcaagtaatt ctttcgtaaa atgcaatcca gctgttggcg ctgcagcaga accattttct
     4681 ttcgcataga cagtttgata gcgatctggg tcttccagac gttctttgat atatggaggc
     4741 aatggcattt ctcccaatga ttcaagaatc tctagaaaaa ttccttcata ctcaaagtca
     4801 atgatccgtc cgccgtgttc caattcttct ttcactactg cttgcaatct gccatcacca
     4861 aaagagattc ttgtcccaac tttggcacgt ttcgcaggtt tgatcaatgt ctcccacgta
     4921 tctccttgtg tattcgttag aagaaggact tctaaatgcg caccagtatc tggtttttct
     4981 ccatataaac gagcaggcag aactcgtgta ttattcataa ctagcgcatc accaggctgt
     5041 aactcttcaa tgatttcatg gaaatgttta tcttccatct ctccagtttt ccgatccaaa
     5101 acaagtaaac gagaatgatc gcggtctttt aatggtgttt gtgcgattaa ttcttccggc
     5161 aagtcaaaat caaaatcttc tgttgttaac atatcgtcac tcactttcta tcatgtgctt
     5221 catcattcta tcatgttttt cgttctgttg gcaaaatcag cactttttca cttctatttt
     5281 ttgttttcct aaataaaaaa actaccctat ttgtattttt tcattgtttt aggcatacta
     5341 gtaatagata gttcttcatt gaaaatagaa aaagaggtgg caccatgatt gaatttcaaa
     5401 atgtgtcaaa aatttataaa ggtggaaaag tcgctgtaga ggacgtcaat ctttcatttg
     5461 aaaagggtga gttcatttgt tttatcggta cgagcggaag cggaaaaacg acgacgatgc
     5521 ggatgatcaa ccgaatgact gatccaacac aagggaaaat tttgattgat ggaaaatcca
     5581 tccaagaaat tgatccagtg gaattgcgac gtcagatcgg ctatgttatc caaagtattg
     5641 gattgatgcc tcacatgacg atccgtgaaa atatcactct tgtccaaaaa ttgttgaaag
     5701 tcagtcaaga agaacggaat aaaacagctg aaaaaatgat tgatcttgtg gaattgcccc
     5761 gtgagatgtt agatcgctac ccacatgaat tatctggtgg tcagcagcag cgtattggag
     5821 tcgttcgtgc ccttgcagcg aatcaagata tcatcttgat ggacgagcca ttcggtgcgc
     5881 ttgatccaat cactcgtgat tccttgcaag accttgtcaa agatttacaa gagcgtttgg
     5941 gaaaaacgat cgtatttgta acccatgaca tggatgaagc gattaaacta gcaagccgta
     6001 tcgccatcat gagtgaaggc cgtgtgatcc aatttgatac accagaaaat attttacgtc
     6061 atcctgcaaa cgatttcgta gaagagttga ttggagaaga tcgcctacta caagccaagc
     6121 caaatgcgac aagagtcggc gaagttatgc taaatacagc aatcacgatt acaccggaaa
     6181 aatcattaca agaagcaatc aaactgatga gggaaaaacg tgtagatacg ttgttagtta
     6241 ccgataattc gaatgtatta aaaggattta tcgatgtaga aacgttgaat aaaaaacgag
     6301 gaaaagtatc cagtgtcggc gacattttga ataaacatgt tttttacgta aaagaatccg
     6361 cctatcttcg tgataccttg caacgcattt taaaacgcgg gctgaaatac gtgccggtcg
     6421 ttgatgaaca gaacaaagtc gtaggtatcc tgactcgtgc atcattagtg gatatcgtct
     6481 acgatgtgat ttggggagaa gatcccgcaa ctactgatgt tgcagagtca gctactcctg
     6541 aagaatctaa ggaggtctga cctatgcagc agttttttca tcagcacggt tcagaaattc
     6601 tctcaaaagg ttttgaacat ctttatattt caggacttgc tttgatcatt ggtatcatca
     6661 ttgctgtacc gcttggtgtc ttactgacac gttttccaaa agctgccacg attgtgattg
     6721 gcttaaccag tgccttgcag acaatccctt ctcttgcttt attggcattg atgatcccgc
     6781 tatttggcgt aggtaaatta cctgcgatta tcgcactctt tatttattca ttattgccga
     6841 ttttacgtaa tacgtacatt gggatgaaaa atgtcgattg gaattatcgg gatgtggcaa
     6901 aaggcatggg gatgactgag ttccaatcca ttttttccgt tgaattgccc attgctatgc
     6961 caacgatcat ggctggtatt cgtttagctg ccgtttacgt gattgcatgg gctacacttg
     7021 cctcttatat cggcgctggt ggtttaggcg atttgatttt tagtggactg aataattacc
     7081 aaccagatct cattttggca ggtacgatcc ctgtaacaat tttagcacta ttggctgatt
     7141 ggcttctagg tctcttggaa caccggctga caccgatcgc acttagagag gagaatgaag
     7201 aatgaaacgt atactaaaaa tgaccatggt tcttttctct cttcttatac ttgcgagttg
     7261 ttccttccct ggtcttgcca gcaatacaga tgaagacacc atttcgatca caggtggaat
     7321 cacttcagaa gcacagatct tagcaagtct agttgccgga atggttgaac attatacaga
     7381 taaaaatacc gcaatcatca acaatctggc tactacaact atcaaccatc aagcgatgat
     7441 gaacggagat gcttcaattt cagcggctcg ctacacagga acagatttaa cgactacatt
     7501 gaaccttcct cctgaaaaag atccgaaaaa agcgtttgct accgtgaaag atgaatttga
     7561 gaaaagatac ggtcaaacgt ggttcccgtc ctatagtttt gaaaatacgt atgtctttct
     7621 tgttcgaaaa gacaccgcac aaaaatacca tctatctaaa gtcagcgatc tgaaaaatgt
     7681 agcggatgaa ttagtagctg gtgtagatac ttcttggatc aatcgtaaag gagacggtta
     7741 tgacggcttc caagaaactt atggcttctc cttcaattcg atattaccta tgcagattgg
     7801 acttgtttat gacgcagtcg aagcaggaaa aatggatatt gttcttggct actctacgga
     7861 tggacggatc gccagttatg atctagtcat gttggaagat gatcgtcaat tcttcccacc
     7921 atatgatgct gctccagttg tagacaacaa gatattagct gatacgcctc atttaaaaga
     7981 agccttaaat aagttaggtc ataccatcag tacggaaaaa atgcaggaat tgaactacga
     8041 agcagataat aatctagtgg aaccatcagt agtagcacaa cgtttcttac aagaaaacca
     8101 ctattttgaa gggaagtgaa gcaattggat aaaatgaatg tctttcaaca atttttctat
     8161 tatttccagg aaaatggctc ctatatcttt gcacaattta tccgtcactt cctgatttct
     8221 atctatggtg tcctctttgc agcagttgtc gggattccgg ttgggatcat gatttctagg
     8281 agacgtaaat tagcaaactg ggtgattcgt ttggcgaata tcatccagac gatcccttct
     8341 ctagccatga tctccatttt aatcattgga ttaggcctgg gagtcaatgt cgtgattgtt
     8401 actgtatttt tgtattccct cttaccgatc atcaagaata cgtataccgg gatgatccaa
     8461 gtagacaaaa acatccttga tgtcggcaaa ggcatgggga tgactgctag acaacgacta
     8521 ttcatggtag agttaccact ctctgtttct gttatcatgg caggcataag aaacgcgtta
     8581 gttgttgcca tcgggataac cgcgatcggt gcattcgtcg gtgctggtgg tcttggagat
     8641 atcattatcc gaggaaccaa tgcaacagag ggcacatcga ttattttagc aggtgcattg
     8701 ccaactgcat taatggcgat catcacggac tggcttttag gtattttgga aagacggcta
     8761 gatccagcca gccgtacatc acgctgatgt tattgtaaaa aacaaaagct tctgcgacca
     8821 tttgttggtc gcaaaggctt ttttagttct gtcattttta aggcaataat ccactctgat
     8881 tcttccctcc ttatgggcaa agagttgctt ttcttagcta tttatccatt ccgtatactc
     8941 caacttcata ctgccaacgg gttcatatct gcatccaccg actaagtttt tttcttatcc
     9001 gctaaggcat tttgccaatc cattttccca tcactcgtca tggaattcat accacatgat
     9061 ctctaaatac tcgtctgctt gttctttgaa ttgggcatca attcttacat cctttggcgt
     9121 ggctttcatt taagcctttt ccatcagtcc tcgattgttc gatgccttca gctgcatcat
     9181 caaaactggt gactgccgta tcggtaactt cgatcatctt ttatacaatc gggaacttgc
     9241 catcctcatc cttggctaaa aacatatcta gccattccag cttctcgcca tgggaggttt
     9301 gcggttccta aaaccggtac acgattctta tggactgcat ccgtcgcaac tagacttggc
     9361 gatacgatga tccttcacca gaagagcacc cccgtaaacc aattgatcga tatattgcca
     9421 ataagaaaag acattggcgt aaaagatatc gatccctcgt gatgcattgc cgttgatact
     9481 gctattcatg gccgaaagcg ccacgacttt aacttcttcc gtttgagtga cattcatgtt
     9541 cggcaatgtc gctttggata cccgctctgc taaaggaacg acactggtat tgtatttcgt
     9601 atctgtatct tttccaaaag accatgccaa cgaatcctct gggaaccaat aagacgactc
     9661 cggttgattg tccattgctt ttttcaacat acttttggct gtttctcgat aaacgacatt
     9721 tccaattgtc ttcgcctcac tttggcgata atcgatggtc gttgccactg ccaacactaa
     9781 caacgatgct ccaattttac tgattttcct tgtcacactt cttcctcctt gttttacata
     9841 aacccgtcaa cattccattc ctaaactcaa acaatgaccc ttcaaaggtg gagatatagg
     9901 aacaaattaa agaaatcatg caataaatcc aagaaggttg gttttagaac aaaaaaagag
     9961 tctccttttt ggggagactc tttgttaact atctaaaaag gttggctctt ctcacttcac
    10021 tcataatgtg tccaggcaga ataaatttct acaactttat tttcctcatc aactttgtaa
    10081 accactcgat gctgtctatt cagcctgcgt gagtatctgc cttcatgtgt aggtctcagc
    10141 ttttcaaaag actgcgtggg catatatgga tcttccttca atgtctgaat cacttcttca
    10201 aattgtttct ttaaattcgt ttgcttgatt tttcttaaat ccactttcgc agagtttttg
    10261 attgcgactg tatagttact catcaaagag cttcccaatc aatatcatcg atattcgtaa
    10321 agctactgtc gtctttttcg cggtctcgta ctttatctaa agtacctgtt tgctcaagaa
    10381 aaagggtttc ttgaatcgct ctccagtctt ccagcccaat caatacagca ttgtttccac
    10441 tacgatcact aataatctcg atttcagtat gattttcatt tacctctttc aataactgat
    10501 aaaaattttt acgagcagtg ctaggattca tcgttgtatt agccattgtc tctcctcctt
    10561 tttctgatat aattatagcg tacttgatta agtacgtcaa aaaaatacaa taaagcactt
    10621 aaattcagca aacttttcct cttttttaca tagagaaaaa gacccctttt tagggaggat
    10681 ctttgtccac gctcacttct tactccttta cgactagcgg tagccaaacg taaagtaatt
    10741 ggtaacaacg tatcatcttt agcaaaggtc gaatccaaag cctgtgagtc tatgcaacaa
    10801 caccaataca cgaaggttcc tcgttgcagt gtgacttgat tgatatcttg aataacctga
    10861 ggatacgcac gaaagatttg taacggttat tcaaaactga ttttggccgt caagtcttct
    10921 actgcctcaa aaatgattgc ctctttcgtc tcctgtcatt catgttcact atcgatttga
    10981 tacttgctta gaccgttggg tttgtgaatc accaattaaa aatggcactg agttatggta
    11041 acaaaaaatg gtcacttgac gaggctgaga ttcctactta tgaagacaca tggcacttaa
    11101 gagtcgaggt aaaggaagaa gctgaaaata taggaggctg tacattattt ggcaaaggat
    11161 tcggaaatta tgatcaagac atcaaaatca aaatgtttta cagctaaata ggaagaataa
    11221 taacatttca tacgctgttc ccatctttaa tggcttatag agggcacctt tagctgagaa
    11281 taagacaaaa cagaccctct agcttcaatt aagagctaga gggtctgttt ttgattttaa
    11341 aacttggttt atttcttcaa atggtagcta taggtgctga caacgatatt ttcacgccaa
    11401 gacaatcgca atcttaaccg caaacttgtt tggttatgga gaatattctg ccagcgtctg
    11461 tttggtacaa attgaggaat caacacagta gttgtatagt tttgtttcgc tgcattttta
    11521 cttacgatat ctacataacg gataattgga ttttcaatcg agcgataaga agagtggacg
    11581 attgaaaaac ggacatctgg gaaatgcttt ttaaattctg cttcgatttc tttttctttt
    11641 tcgacatctt cgtctagcga aacatgcatc gcaacgacat aatcaccgat cgaacgtgcg
    11701 taattcaacg caccgatatt tacccgtgtc acattcccga ctaatacaag aacggtattc
    11761 ccgtcataat catgtagttt tgcatcattt tctaaacgaa gctgttctgc tactttttta
    11821 taatgatcat ggatcttata aaaaatgaac ataacgattg gcataatgat gaagaaaggc
    11881 caaatgtccc ctaaccgata gacaaacaaa atcgcaatga ttgcatatga gatgaacgca
    11941 cccgtaatat tggcaatcga tttgcttaac catctttttc cttctttttt ccatttgacg
    12001 accatccctg tttgagataa agcaaatgga atgaacacgc cgatagagta taacggaatc
    12061 aagcgttctg ttgagccatg gaaaataaac agcaatacga tcgaacctag cgctagtgtg
    12121 ataatcccat tggaatagcc tagtcgatcc ccacgatctt gatacatgtg aggcatgaat
    12181 ttatcttttg ccagattgta tgcaagaact ggaaaagcag aaaaaccggt gttagcagca
    12241 actgctaaaa tcagcgctgt agcaaattga aggacgtaat acaaaatacc atgtccaaag
    12301 acagctttac caatttgaga caaaacagtg acttcttttt caggaacgat cccataccaa
    12361 tagttgataa atgtgatccc cacgaagaag aaacctaaga tcaatgccat catcgtcaat
    12421 gtagcagcgg cattttttgc tcttggcttc ttaaagaatg gtacagcatt actaatcgcc
    12481 tccaccccag ttaaagagga agaaccagaa gaaaatgctc gtaaaatcaa agcgatagaa
    12541 acacccggca cagccgctcc tggaagtgca gtagcttgca atggcacagc acctgtcacg
    12601 atattatata aaccaacaac gatcagtaac gagatcacaa ggataaatgt gtacaccgga
    12661 aataatagga acgatgcaga ttccctcagc ccacgaagat tcagcatcat cagtagcaaa
    12721 acgattgtca cagaaatagc gacttgatga ccgtagagtg ctggcaccgc tgatgtgatc
    12781 gcttcagcac ctgctgagac agatactgct actgtcaaca tgtagtcgat caataaagag
    12841 ccaccggaaa tcaagccagc attctttcca agattctcac tgctgacgac atatgctccc
    12901 ccgccatgag gataagcatg gatgatttga cgatatgata gcgttaatga aatcaataaa
    12961 ataataacaa aagcggcaat gggtagcgaa taccagatgg ctgcagcaga caaagcgact
    13021 aaaacgacaa cgatttgttc cgtaccatat gcaatagatg ataacgcatc agacgacaat
    13081 aatgccaaag cagcaaatcg ggttaatttg tgctcatcat tttcagcaga ttttaaaggc
    13141 ttacctacta acaacctttt taagtaatcc aatttctaaa ctcctttaca cacaatttta
    13201 cttcaagttt tatagtcgaa aatttatagt aaacaagatg ttatagtacg ctcactgacc
    13261 ttaaaagtca ataaacttta agaaaataat tggaaaaaaa cagaaaaata tcaagcgctt
    13321 tttcaaacgc tttaatacag caaaaatcta acattttttt atgggctttt tctttgaatt
    13381 ctataataaa aggatagaac ttgacttaga aaatcaagtt ctatcctttt tgatatctta
    13441 acttttggaa gagagatgta aggctgcttt ctacccatca ttcttactac tcgaagcata
    13501 gcgcagttgt cacaacctct tgataaacgg tgtttaaaag cttgcatctg cggtaataag
    13561 cccaaccaaa ttaaaaacat aagaagctgt ttttatttgg tcgttcttat tacttgatgc
    13621 agagcagctt ttttacaacc tcttggtaca cggtgttcaa tcagctgacc ctcggtatta
    13681 gctcaaaaaa acggcaaaat atgaggaact atttttccat ttttttctct aatactcagg
    13741 tcaaaacact gatttcacaa cctctttttt taatgaaaaa tatggctctc atgcgccgct
    13801 tctttgaatt tcgtcatttt ttcttcgatg aacggataga ttgccgttcc tacgattcca
    13861 aagacaacaa acaaaccgat catgatccag attgctttcg tagcatttgg ccaataaatt
    13921 cctcctgcag attcccgtaa caagtttacc gcatgagtaa atggcaagaa cggattgatc
    13981 atttggaaga atttccctga gacttggatc ggatagttcc cgcctccgcc agagatcgac
    14041 agtactaaga tgataatagc gatcccttta ccgacattat cgaataatgc agccaagaca
    14101 tagacgatca tcatgaacgc taaggcgatc aagacagcaa atgacacgct gtagaccgga
    14161 ttttggacat ctactcctag taagaaaata ttccctaatg taacgatcaa gctttgcgca
    14221 atcgccattg tcaaaaatgt cagcatacga gcaacgaatt gttctcgttt cgtaaaattg
    14281 tttcggtctt tctttcctaa atagtattct gtcgtagcaa cacttgaaag aagaacagcg
    14341 ccgacccaca gacatagtgc agtataaaac ggggtgctag ctgaaccgtt atttgcgatt
    14401 ggataaagcg cattggtttg tagctcaaca ggttgtgtaa agaagtcact ttcttttgtt
    14461 gcgtctagtt tcaataattt gatgacttcg cctagatcga cttcttgttc gccttttcgg
    14521 atcgcgtctg ctgctttttg gataccagct ttgatatttg gccagtcatt gttgatcaag
    14581 tccgcagcag cgttcacagc tgtttcgact tctggcattt tttcatttac tgttgtcaat
    14641 gtaccggtga tttcattttt tagtgctggc caatcatttt ggataaaatc agcggccaat
    14701 cctaattttt gctgaatgat cggtaattca ttgttgtata ggtctgcccc ttcattgatg
    14761 ccattgacga tggtatccat atttccattc agcattgtat tggcatcatg gatttcttgt
    14821 ccaattgcag gcatttcttt ttgatatttt tctaaaattg ccactgcatt cgttacagtt
    14881 gctttcgttg agctgagaag cgaatcaaag tcgatctggt ccgctttgct cagtgcgcct
    14941 tgtgcagtcc caatcgtatc gatgacttgg tctagtagag aagtgacagt ggaactgatt
    15001 tgacctgtat tgataccagc aatgatgcca ttgatattat taacaacggc atcaatttga
    15061 tctagcaatt catcaatagc cttcgtgtct tcattttgaa cagctgtgtt caaagcagat
    15121 aggcggtttt gcagattgct tagcaagggt tttaattgat ttagctgatc aataattggc
    15181 tgtaatttcg tatttccagc acttgtttgc aatttagtca ggaactcgac cagtttatcg
    15241 attgcatttt gttgttgaga tattccgtca ataaagccgt tcaatacatt gctgatgttt
    15301 ttcttttcat catcagtcag cttaccatct tcaatagctg ttcttacagt atctattaac
    15361 gaacctgtcg ttgttgccac ttgtccaatt gacttcaatg taacatctac actgtttgtg
    15421 atgcttggta tcgcatcttt cagctgagag gcacctgatt ttgtttggtt tgctagatca
    15481 cttgcctgat tttctaaatt tttcacatct ggcaagatat cttgtacttg ttggatgatc
    15541 gtcaagcctt gttttgcttc atcgattcct tggttcattg tctcttggat cgaagcaaaa
    15601 tcgccgtcga tctctgctag ttgcttaccg gcattttgga tctctgggat tttttcttgc
    15661 aaatcaagga tgatttttgc ttgatctttc aactcaggca ttttgtcatt caatgcaacg
    15721 actttttcac ccatctcatc tactttaggg atgtaatcca cgaattcatt tgctttattc
    15781 agtttttcct tgatttcagg tagttgactt tgtaattcga ggacttgttt tgtgtagccg
    15841 tcgatcgtgt ccaaattttc atctgttgat aaaatcatat ctttaacttt attgatactt
    15901 actaggttcg tatcgatgtc gtaacctatc tcattgaata ctttcaataa agtcgaactg
    15961 gctgttttga taaattcatt tgtgatttgc gattggatcg aacttgctcc tttatcggtg
    16021 attttcggtg caatcgcatt gattttttca tttaccgtat attcgatttt tggtttcttg
    16081 atatcaccac tagtaaaact cagcagatct tctgagaaat cctttggcaa gtaaatacct
    16141 gcgtaatact ttccggaacg gactccgtct tctaattgtt cttttgaatc aacgaattgc
    16201 catcctaatt gtttattttt atgaagcgat tcgatgactt gctccccaat ggccacttct
    16261 ttaccttgaa actctgctgg cttgtctgca ctgtaaacag caattggcag ctctccagta
    16321 tttccatatg gatcccataa tgctttgatg ttaaaccacg catatagcga agggatgatc
    16381 atgatcgcga tgatcagaag tgttgcaatc gggtttttga aaatccgttt ccagtcaagc
    16441 aaatataatc gaagcgtatt tttgattgac tgcatagttc tcccccttct tcttttttca
    16501 gctgaattac tttaccatgt atttcatact tttttcaatc ggcttttttc aaaaaagtcg
    16561 aatttttgga catatcacgg caattgtctc attgtaatct cttacatatt gagaaataat
    16621 taaggattac agcctttact ttatcatgaa ataaaaggga aagataattt tatgccaaag
    16681 tttttatcta ttatacgtat acgcaaaaaa gctaaaacat tttttgtctt tgcccctaca
    16741 aaaatgtttc agcttttttc gttttttatt cctgtatttc ttatttttcg aatgacagga
    16801 tgattttccc agcaggagga tgtccttcaa ctagctgatg tgcttggatg acatgcttaa
    16861 gtgaagcaga cagattcatt gaaataaaaa catgaaaggc tcctttttgg taggctttga
    16921 ttatgccagc aaaggcttct gcatctagat attcctttct tggaacaaac gattcataaa
    16981 agccttcctt tttttgccgc aaatctagat ctgggaggtc atttaatgca acatacctgc
    17041 cgcctggttt catgatctgg atgccggttt ctcctttgat acttcctttc gtcgcatcga
    17101 ttacaaggtc tgcctgatca gcaaattgca gacctggatt tgttttatca taagcagcaa
    17161 atgctgaagc tcctaatttt tttacctttt cctcattttt actggatgca ctggttaaga
    17221 ttcggattcc cttttcatgg agaagctgga tcagacttga cccgaccgca cctgatgctc
    17281 ctagtatcat tacagtatct gtcggctgga tttccgtcaa atggttgatt agattataag
    17341 ccgtaattcc tggcgtgacc attccggctg cttcttccca actcatttta tctggaattt
    17401 ttgctacttt tgctgatggc aacacgactt cttctccgta agtcccgccg actgcatgga
    17461 cagctactcg atcacctaca tgaacagttg tgacatctga accaatctct gtcacaatac
    17521 cagcgccatc attacctggt acataaggga atttcaatgt tcgtatctct ttcatttctc
    17581 ctaaacgcaa ggcaacatca taaggattca cactgaatgc ttttatttct acacgaacat
    17641 gtccagaacc gacttctctg ggatgagcat cgatttcttc aaaaacatct tgtgcttctc
    17701 cgtaaccgtt aattgcaaat cttcgcatgg tcaccatctc cctttttctt taagtttacg
    17761 tttatgcctt tattttgaca aaaaaaatgt cttttcgttt tggcgcatga ttcaaaacaa
    17821 aatagttgtc ttttttcgcg taacgtgtta aattatttat ctaaaagcgt aaagattacg
    17881 atttacgcta ataaaaaata attggaggaa tttgttgatg gctaaaaaat caaagattgc
    17941 caaagcaaaa aaacagcaag aaacgattga acgctatgct gctttacgcc aggaattaaa
    18001 agcgcaaaaa gattacgaag gcttgcgtaa acttccttta gatgctcgtc cagaacgatt
    18061 gaaaaatcgt gatttaatcg atggacgccc tcgcggatac atgcgtaaat tcgggatgtc
    18121 ccgtataaaa ttccgtgaat tagcacataa aggtttgatt ccaggagtaa aaaaagccag
    18181 ctggtaatcc aagtctggct gtaatgtaaa ggaggagggc gcatggacca ctccttggat
    18241 catcaaaaaa gatacgttcg cttcttctag cagaaatgct ggtactagaa gcagacgtat
    18301 ctttttttac gcacagctgg tcctttttta tatgtaaagg ctgcatggtt acttcgctat
    18361 atttatttag taacaggagt cagtgcttcg atacccacac taattttcaa tgctcggtca
    18421 acttctgcca taatgggtcg atccaaatgg cagacctttt ctttcaatct tgatttatcg
    18481 atcgttctga tctgctccaa caggataacg gagtctcttt ctattcctgt tccagtagct
    18541 ttgatcccaa tgtgagtcgg aagcttaggt ttagccattt ttgccgttat ggccgcaaca
    18601 atgatcgtcg ggctaaaatg gttacctaga ttattttgaa taaccaaaac tgggcgtgtt
    18661 ccgccttgct ccgatcccac gactggagaa agatcagcaa aataaatatc tcctcttttt
    18721 accataggat cctccttatt cttgatattc tcttggtatc cgctgtccta atccgcaagc
    18781 cacttcatag tggatcgtac ctaattgatc tgctatatcc tgcatagtga tttctttgtc
    18841 tgcatttttg ccgataagcg tcacttttgt tccgacagga aattcttgag gcaatcggat
    18901 catcagttga tccatacata cacgcccaat cgtttcacag tagttccctt ctaccaatag
    18961 agaaaaaccc tgcatctttc gtggccagcc atctgcataa ccgatcggta tcgttccgat
    19021 ccactctgct tcaggagtga tatatgtttc tccatagccg attccttcac ctgctgggag
    19081 ttttttgact tgaatcaatt cagaaaccag ctcaagtgct ggctgcaatg gataaacttc
    19141 cggtagtgcg tgcccagagg gattaagtcc gtacattgct actccataac gaatcatatt
    19201 gccgattgtt tcatcatgcc ataaagccgt tgcactattg ctgacatgta catatcgagg
    19261 aagttcggga agttttttca gcacttctat aaagcgttct ttttgcagat tccaatatgt
    19321 atcatctgcc tgatctgctg tagaaaaatg ggtaaataaa ccttcccatt ctaatacacg
    19381 actttctttg atgaatgcag ctgcctcttt tacttcttca ggtgtacaaa atccgatacg
    19441 acccatccca gtgtctgctt ttagatgaat cgaaagaggc gtctctagct tacttttctc
    19501 taaaacagca gctgctgctt gcaaccattc ccgtgtacct gcagttactg ataaatcatg
    19561 agcaacagct acagtcagtg cgtcgtaagg aaccatattc aagataagga tgggctgagt
    19621 aaatccagct tctctcaact cgactccttc atctaaattt gaaacacaaa agcccgaagc
    19681 tccacctgca actgctgcct cagctgtttc aatagctcca tgaccgtatc cgttcgcttt
    19741 tactactgca aacaattctt ttccttgcgg aagtcttgca acttcatttg ctacattttc
    19801 agttatagct tttttatgta tcactgcttt cgttgggcgg tgccaagcga caaccatcgt
    19861 ttttccttct ttctagtctt ccaaaataat ttgtgcaaaa gctgtttcat ttgtatgagt
    19921 aatactgacc catacattgc cttcatgagg tgaacgcgtc acaacaggct gaccattttc
    19981 attttttaaa atctcgacat cttgaaaggt aacttttccg atcccagttc cccaagcttt
    20041 agagaaagcc tctttgcaag cgtaacgccc gcctaaaaat tctacttggc gatgaaaagg
    20101 caaggactgg aatagtttca tttcatcaga ggtcaagatt cgtgcaatga attttggttt
    20161 ttcttcgatc agccgagtga tcctaggaag ttcgactgca tcgatcccaa ttccttttat
    20221 catatgcttt tctcttttca ataaatttag aacactctca ttctaccaaa ttataaccat
    20281 tttgccacaa ataagtccac aaataacaaa aatgaaagtt tctcttaaaa aagagaaact
    20341 ttcattttat gtttaatcgt tatttgaacg gattacaaat ccgcgttttt tatcattgtg
    20401 acgtttaccg ccatcttttt ggctgttatt atttttgcta taaccgcctt ttgatttgtt
    20461 tccacgataa ttaccgccgt cacgacgatt acggttgttt ccgccaccac gtttgccatt
    20521 tttattgtat ccttttttct gcattggcaa tgggcgttct ggtgtgatct tcactggcac
    20581 tgcatcagct ggatcttttg ctgtcgtttt tagcaaaagt gcaactaaat cttgtgcaga
    20641 atattcttct aataatttat ctgctgtttg caggtattta tctagaccat tttctgcaag
    20701 ttttgtttcg atttgttcaa ctgcagcacc taattgacct ttgaatgctt ctttttcggt
    20761 tggtggacgt aaagtcgtca tccgtttttt cgtcaagttt tcaatcacat gcaagtagtc
    20821 catttcattt ggtgtaacga aagtcacaga cataccgcct tttccggcac ggcctgtacg
    20881 tccgatacgg tgaacgtagc tttctggatc ttgagggata tcatagttgt acacatgagt
    20941 gacacctgaa atatctaatc cacgtgcagc aacgtctgtc gctacaagga tatctaaatg
    21001 accgctctta aatgaacgca atacgctcat tcgtttttgt tgagacaaat cgccatggat
    21061 tccttctgca cggtaaccgc gtgcttctaa tccgcgtgcc aattcgtcta cacggcgttt
    21121 tgtacgtccg aagacgattg ttaattcagg tgtttgaaca tcaaacaagc gagtcatgat
    21181 atcgaatttt tcgtattctt ttgcgcggac atagtattga tcgatcaagt cagctgtcat
    21241 ttctttcgct ttgatcttca catgatgtgg atttttcatg aatttaacgc caatattctt
    21301 gatagctggc ggcatcgttg ctgagaaaag taatgtttgt ctttgatcag gaacttgtga
    21361 aatgattttt tcgatatctt ccaagaagcc catattcaac atttcatctg cttcatccaa
    21421 aactaatgtt tgaacggttc caagtttcaa tgtgtggcgg ttaatgtggt caagcatacg
    21481 tcctggtgta cccacaacga tatgtggacg atctttcaag ccacggatct gacggccaat
    21541 atctgctccg ccgtaaacgg cttgtacacg tacttttttg tcgcgcccta aacgatataa
    21601 ttcttcttgt gtttggatag ctaattcacg tgttggtgcg ataactaaac cttgtaattc
    21661 gtgacgatct ggatcgattt tttctaacat tggtaatcca aatgcagctg ttttccctgt
    21721 acctgtctgt gcttgtccaa taacgtcttt tccagctaat gctagtggaa tcgtttctgc
    21781 ttggataggc gttgcttctt caaaaccagc gcgttcgact gatttcaaca attctggtga
    21841 taattctagt tctttaaatt tcaaatgaat cctcctatat ggttgttaac tgaggatgta
    21901 gcttttttgc taccccgtcg atttgttcgg tcaaagtgta ttgtttcttt ttgacagtgg
    21961 ccacgcacgt ttttatccat tcttagcccc tgtggcacca ccgaaaaatc actttgcgaa
    22021 gtttaggcat tagaaaagcc aaacgggtcg cgttcagctt tatcattatt atgacatctg
    22081 gcttcaacaa gttgtttcta tggcaaaacc agtccaattt ctcttcatct ttcggcaata
    22141 ggttattcta acatagtttt tcgttttctg caagtttaca gatttttatt tcatttgctg
    22201 aagttgttct accacttcaa ataagcccat gctgttgctg ccttttagca taatactatc
    22261 tgttgcttca agattttgtt tgatagcatc aatcaattta ttcttttctt tttcaaaata
    22321 gaacacaggt agttgggggt atttttggtt caattcttct tttagtgcat gcatttgttc
    22381 cccataaagg aaaatcatgg cgaatttctc aggatcgata tgttcactca tttgcgcatg
    22441 catatttaaa gactctttcc ctaattccag catatctgct aacacagcga ttctgcgtcc
    22501 ttttgttggc agatttttaa aagtatctaa taccagtccc atggctgttg gatttgcatt
    22561 gtagacatcg ctcaaaatag ctgcaccatt tcctgccgtt agccattcgg ttctgttttg
    22621 agtgatagaa acatgggcga gcccttggcg tatctcaggt atggacagtc caaagaagcg
    22681 cccgatcgta tacgcaatca aagcattcgt tacattataa cttccaggca gcgggatcgt
    22741 aaatgtttct ccttctacta caaaatcggt ttgtgctttt ccttttgctg tcacttccgc
    22801 atgaacatcg ccatttccaa taccgaatgt tgtgactgtt tgtactaatg ttgccgttaa
    22861 aggtgtcagc aacggttcat ctgcgggcac aacgagtaat ccgtctgata caagtccgtc
    22921 agtgatttcc attttggctt tagcgattcc ttctcgcgac ccaagatttt ctatgtgtgc
    22981 ttcaccgatc attgtgattg ctgcagcttt tggttgagcc ataagtgaaa gttccgtgat
    23041 ttcattggcg tgatccatgc ccatttccaa aacgatcttt tctgtatctt ggggcatgtg
    23101 caaaatcgtg taaggaagac caatattatt gttgtaattt ccttgcgtct tatatgtttt
    23161 gtatttttca gacaagacag acgcggttag atctttcgtg gtggttttac cattgcttcc
    23221 tgtaattgca acgacttctg cgcctacttt ttccaagtaa taggttgcca attgctgcat
    23281 ggctttcaaa ggatctgcta caggaataac tggaaaattt tttggagctt gttcaacagg
    23341 caagctccaa aatgaagcag atgctccgtt tgtgatcgct tgctggataa attcatgtcc
    23401 atctcgcgta ccagcaagag gaacgaataa acttccttcg gttatcttcc ggctgtcaaa
    23461 ttcgacattg gtaatctttg tcagtgcaga gcattcgctg cccacggctt gtgcaatttc
    23521 ttgtattgtt agattcattg agatctctcc taaaaaaggt tgtgaaaaaa gcgtttggcc
    23581 taaacggcgg gttcgcatga ctctcacttc caagtaaatt tctctattcg tatttagaag
    23641 ctagaatgaa cgcggttacg caatcactcc agcctcttct ttatttctct ttattcattt
    23701 ttttcaaaaa aagattgacg ctgatggtat cgattcattc ctaactggat cagttcttca
    23761 atcaaatcac cgtatttcaa gcccatattt tcccataaga gtgggtacat actgaactcc
    23821 gtaaatcctg gcatagagtt taattcattc aggaataatt catttttatt tgtcaaaaag
    23881 aaatcgcacc ggctcaatcc gcttccacct aacatcgtgt aagctaactt cgcgtactct
    23941 tgcgcttttt gataaacttc ttctggcact tcggctggaa tctgcatttc gattttatta
    24001 ttgatatatt ttgcttcata atcatagaat gctacgtctt ttacgacttc accaggcaaa
    24061 gtcgtccgaa cgtcttcatt tcctaataca gcaacttcga tttcgcgcgc ttcaattcct
    24121 tgttcaacga ttgctcgaga atcatactga taggctgttg ctaaagcatt ttgcagctct
    24181 tctcggtttt ctgcttttgt aatgccgaca ctagaaccca tattcgccgg tttgacaaac
    24241 atcggataaa gcaaagaacc ttcacattga tcaaatactt ttttaggatt ttctttccat
    24301 tgattcttaa gtactggtac ataaggaact tgcggcacac cagcagcttg taaaatatac
    24361 ttggtcatga ttttatccat ggcacatgca ctggtcaata cgcctgcgcc gacataaggc
    24421 atattcaatg tctctaagaa gccttggatc gttccatctt ccccgtttgg cccatgtaaa
    24481 actggaaaaa cgatggctcc ttcttctttg atttcgcccg gattgatcac ttttcctgta
    24541 aagccttcct ctgtctgtcc acttgggtcc catgaaagat gcaagacatc tttgctggca
    24601 ggtttttctg ttagtaatgg acctttgacc cattgtcctt cttttgtaat aaatacgagt
    24661 tgaacttggt aataattata ataaatggca tttaaaactg aaaatgcgga aagaatggac
    24721 acttcatgct ctgcgctgcg tccgccatat agtaaagtaa tcttcaaaag attgtcctcc
    24781 tgtattctaa atcgttcagt gatttactct catattttag cacaaaacaa aaataaacga
    24841 ataacttatt tataaaaaaa cgagccagct tctaaaagaa cgaacgtcat ttattttttg
    24901 tcagctagat tatttgtccc gttttttata aaaagttatg ataaagaaaa ggagggatga
    24961 tgatggcgca aacaaatcca cgcagccgct tggcagaaat cattcgagtg ttcatcaatt
    25021 acaatgtagt gccaaacttt gtacagcaaa aaaatcccga acaggtaaaa aaagcctttg
    25081 aagaactagg ccccaccttc atcaagatag gacagatgct ttctgttcga gaagatttgc
    25141 tttcttctgc cttcactcaa acattcaaaa cattacaaga tagtgtacct agcgacacat
    25201 tttcaacggt caaaaagacg atcgaaacag aactttctct ttcattatca gatatattcg
    25261 atgatttttc aaaatcgcct tttgcctctg cttctatggg gcaggctcat cgtgccaagc
    25321 tgaagaacgg tgacgcggta gtcgttaaga tccaacaccc taacatcgcc gaagagatcc
    25381 gacttgatct tcaactattt gaacgagcga tccctttgat caaatacatt cctgaaacta
    25441 gcgtagttga tctaaaaggg gtactccaag aagtaaaacg ttccttgatc aatgaaatgg
    25501 acttcttaaa agaaagtcaa aatggcgaac aattctatca aaagaacaat ggctggaaag
    25561 agatccgcag ccccaagatt tatgacgctt tttgttcgaa aaaagtgatc gtgatggaag
    25621 aaatgtctgg taaaaacttg aatcatttaa tgaacgccga aaacaaaact gaaactttta
    25681 tcactggaat tcaaaacaag cagctgaaac aagaagttgc aaagctttta gttgagaact
    25741 ttatgaaaca ggtgtttgat gatggttttt tccatgctga tcctcatcca gggaatctgc
    25801 tattccatgt actaacaaaa gaagaacaga cacaagctag ccgaaaaact gaaacagtcc
    25861 atgaaaaaga gttcggttcc tttgcttttc gagcctccac ttctgcagaa gaccctgttg
    25921 cgccatatac catcaactac atcgattttg gtatgatggg gcatctttct gcaggactta
    25981 ggcaaaaact cacacaagcc gttttagcgc tgtatacgaa agatgcttat cgaatcgaaa
    26041 aagcggtcct tcgactttgt cagcaagaag gctcgtttga tgaaagtcgg ttccaccaag
    26101 aactgacaag ttttcttgaa cagtattatg attctcctat tgatgaaatc aacttgcaag
    26161 aagtattcac acatgtcgtg acgatttgtc atcaaaataa tctgcagttc gatcgtgata
    26221 tcacgctttt gctgaaagca tttgggacat tagaaggggt gatccgcgtc ctcgatcctg
    26281 aagtttccct catggaagtc gcttcgcctt ttgctcaaca ttattttctc actcatctgg
    26341 atgtagaaga tacattgaaa caaagcggat tagacttatt ggaagggatg aaggctgcgc
    26401 ctaagatccc tcaacagctc caccatctat tagaaatgtg gacttcggga caaggaaaag
    26461 tcaatttgga attgaaaaag caagacaaat tattgagcag gatcgaaagt atgatcaatc
    26521 ggcttgtttt cggtatgatt ttagctgcat tgatcgtagg gtcctccctg cttgtccaag
    26581 cagcacctgt agaaaacgca gaggtcgttt ccttattagg gatatttacg tatgctattg
    26641 ccgcattcgt cattattttt ttggcaatcg atgcattgat ccaaatgtat aagaagagga
    26701 aaaagtagaa aaaggttgtg acagaaagaa tcagcttcaa gaaataagaa ggggttttac
    26761 ataaattgtt tctcaaattt ttgtgaaaat tcggcttatt tttcgaagag gctacttctg
    26821 aaacaccgag aataaaaaat gtgaaaaagc tgttctgcac ccagtaataa gaacaatcaa
    26881 aaagggaaca tggaacacat ttatcctgtt tccctgttcc ctttttgatg tattcgagca
    26941 taaataaaat accttcgtta cctactatct tctgtacttg gtaaacctta gtcaattgcc
    27001 ttttttaacg cttgacgaaa agacatatga tctttctccg taaagggttt tggacccttt
    27061 gtccttatgc cagcttttct taactttcca ctatgataac gttgttctag aagcgtgccg
    27121 atattcgctt cgtcaaattc ttttccagag tgatggaaca ctcgcacacc cttcggcaat
    27181 acaagtgaag ctaaaccata atcttgcgtg atcaaaaagt cattcttctt aaccataccg
    27241 acgattttat aatccgcact atcagctccg cggtctacgt agacgatttg gacatgatta
    27301 gcgtattctt tggtcgtata atggtccaca ctggtcacga taaccacgtc aagaccatag
    27361 gattctccta attttatgac atcttctttt actggagaac catctccatc aatgaatagc
    27421 ctcattcttt ttcgccttct tccataaatg ataaacagtt actaatccgc tcagtaaaag
    27481 accaacattg atccattttg ccaagataag cagttgtaca ctttctatcg gcactgattc
    27541 atggttcagc gtgatcaata atagatggat tggctctccc atttcttttg ctaaaacaaa
    27601 caaaagcgtc atcaataaaa actggtggat cacactccct ttattttgag tagtatactt
    27661 gacaacgatc cggcgatcca ttggaatagc tgtatcagtt tgataatatt ccttatttag
    27721 ccagctgcct gttactacgt gccagaaact aagcagaaag acacctatcg caaaaccatt
    27781 tggaatcaag aggaccaata aagaagaact cagtccaagc aattgcatag ccaatggaga
    27841 ccatgttaaa cgtttcaaaa tccatggtcc gactattttt gctgcaatca agcctgctgc
    27901 ataaggcaaa tacataaaaa tcgaaagttt ttctaatccg tacagtatgc caacataaaa
    27961 actgctaaat aaaaaaagga aattccctag cattccattt agaaaagtaa acagatttag
    28021 ccagcttggc aacttccatt ccttcttctg gtgtcctaaa acaaacacga acaagatgaa
    28081 cagcagcaag aaaccataaa tagcaagatc aaaaagcctt tcatcgaata acaatcgggc
    28141 atttctcaaa agaaacaata agccaaagaa tatcaaaaat aaaacaagct ctcgcgtagc
    28201 taccagattt cttttgatgt ctttgaaatc cagttcgtaa cgcggataat gactaaccgt
    28261 atggtaggcc atgacgtaaa aaagtgtata aatagtaaag cttgctgcaa tctgagtcgg
    28321 tgcaggcaac tcaatcgctt gatacaggag aaatagccag atagctgcaa ataagtactt
    28381 tttcccatcc atattgataa aaccttgcgt cttttcgtga aaattgaccg tggtattagc
    28441 aggtacaagc catgctgcac tcagccctag caaaacactt gaaaaataaa ataaaggaaa
    28501 ataaaatacg cctaatattc caagcaatga accagcacca ccaatcagca gagcgatcat
    28561 cagcaacgtg taactatcta gaccaaatcg tagactgttg actaaaaata gtccagtcat
    28621 ccgaaaggca taaaacagcg taaatggcaa aatcgtcagc caattatgcg agtctgttag
    28681 tcccaaaaat aaaagataag gaataaaaat aaccgtgtta cttaagataa agggcgttgt
    28741 ttgtgtaaaa ttacgcccaa atcgtttgag tttttcgaac atgacaatgg ctccttaaac
    28801 agtcttataa ctaatctgct cttcgatttt agcattaaag gggaaataaa ccaattttac
    28861 agaatatatt cttgtaaaaa aaaatacaga aagaagcata agcttctttc tgcattttta
    28921 cggtacttga ttactcaagc agacaagccc ttctccacaa aagtcttgcc cgacacccct
    28981 gcatccggca ggtgataaag atagaaatca attatcctgt gcctccaaaa tcaattagga
    29041 gctctgtaaa ccagagtcct tctcgacccg tcgcataaat ttactttaac acagttccta
    29101 tgtttatgcg agagcttttt tcattttata atcgaccatt tgaaatcctt gaccgatatc
    29161 tgaaataagt tcttctgtaa tctcaaatcc ttcatgctta taaacctcga ttgcttgatg
    29221 attcccctga ttcactcgca aaaagatctc tctttccttt gtttgcgcag caagttcttc
    29281 ataccagcga aaaactgaag aagtcaatcc ttgaccacgt actttattta gcaaatataa
    29341 tttacttaaa taaatttttc catttatctc ttcataagca gtgtaaccga cagtcttttc
    29401 ttcgcttttt aataaaaagt aggacacacc ttctgtcagt tctttttgaa tctgtttttt
    29461 tgactgataa gttttcagca tgtaatccac ttgtttttga ccaatgatcg gcacaaaaac
    29521 ttccggccat acttcttgta tgactgccat cagctcatcc agttcatttt ctgttacttt
    29581 tattttttcc atacacccac gtcctttttt acattataaa tgaccttgcg cttgatggaa
    29641 taaaaagagc atgatcatgc tatacacacc caccgaccac ggtttcaaga aaatcacaat
    29701 ctgcataaat tccctaaaag ataatttcgt caaaccagct actagacaga tcaagtcgtc
    29761 tggcgcaaag ggaagcagca aggtaacaac tagtactttt ttaatatctt tcgtttttct
    29821 tgtcagcaat tcatcaaatt tctgatactt ttttggcgat aaaataagct ggacaaaggc
    29881 tacgccatag taacggacaa gcaaaaatcc gattgcttcc ccaatcacta ggccaataca
    29941 actatataaa agcccaaatc cattgccaaa catcagcatg cccgctactg aggagatccc
    30001 tcccggcaaa ataggaatga tcacttggat cacttgcaac agaacaaata tcatcactgc
    30061 atacttgcca aattgctgga taaacgcttg caaagacgtg ctcgatcgaa aaatcccatg
    30121 atgataagca taaagaatca acacaaagaa aaaaaacaga ccaataatgg gcatgatctg
    30181 aagacatttt tgtaaaactg atcctttttt agacatctat attccccaca tcttttttca
    30241 tttattaaaa ctgcctcctt atgatgtgca ccccaaaagt tagactagaa atctaattaa
    30301 aaggggtgct ttttttgcgg aaatatacat ttgtttttaa gaaaaaagta gtttcagact
    30361 atttaaataa cgaaggcggc tacaaatatc ttgcacataa atatcaaata aatcgtacac
    30421 tggttagaca ttgggtaagg atttataact atcatggttg ggaaggcttg gttggaggtg
    30481 gcaaaagcta cactacaaaa tttaaacttg atgttataga atatatggaa acaaatggtc
    30541 tttctatcca agaaactgct aaaaaattta atatcggttc aaatagaact ctaagtaaat
    30601 ggatagagca atatgaagaa ggcggtgctt cttcacttga gagccaaaaa aggggcagaa
    30661 aaattagtat gaattccaag ctaaacattc ctaaaaaact taaagatgag tctcttgaag
    30721 aagaagttat tcgtttaaga gcag
//
LOCUS       DACECZ010000020        29109 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.020, whole genome shotgun sequence.
ACCESSION   DACECZ010000020 DACECZ010000000
VERSION     DACECZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.020"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1030)
                     /locus_tag="IWO35_000887"
     CDS             complement(<1..1030)
                     /locus_tag="IWO35_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0315962.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="IWO35_000888"
     CDS             complement(1112..1801)
                     /locus_tag="IWO35_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0315963.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IWO35_000889"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IWO35_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ0315964.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="IWO35_000890"
     CDS             complement(2328..2831)
                     /locus_tag="IWO35_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ0315965.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IWO35_000891"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IWO35_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ0315966.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IWO35_000892"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IWO35_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ0315967.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="IWO35_000893"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="IWO35_000893"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ0315968.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="IWO35_000894"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="IWO35_000894"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ0315969.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="IWO35_000895"
     CDS             complement(6443..7462)
                     /locus_tag="IWO35_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0315970.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8522)
                     /locus_tag="IWO35_000896"
     CDS             complement(7635..8522)
                     /locus_tag="IWO35_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0315971.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IWO35_000897"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IWO35_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ0315972.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IWO35_000898"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IWO35_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ0315973.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IWO35_000899"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IWO35_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0315974.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="IWO35_000900"
     CDS             9753..10397
                     /locus_tag="IWO35_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0315975.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="IWO35_000901"
     CDS             complement(10453..10956)
                     /locus_tag="IWO35_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0315976.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="IWO35_000902"
     CDS             11553..12518
                     /locus_tag="IWO35_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0315977.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="IWO35_000903"
     CDS             complement(12617..13789)
                     /locus_tag="IWO35_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0315978.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="IWO35_000904"
     CDS             complement(13776..14921)
                     /locus_tag="IWO35_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0315979.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="IWO35_000905"
     CDS             complement(14935..15867)
                     /locus_tag="IWO35_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0315980.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="IWO35_000906"
     CDS             15984..17063
                     /locus_tag="IWO35_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0315981.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="IWO35_000907"
     CDS             17060..17689
                     /locus_tag="IWO35_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0315982.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="IWO35_000908"
     CDS             complement(17799..18026)
                     /locus_tag="IWO35_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0315983.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="IWO35_000909"
     CDS             complement(18129..20717)
                     /locus_tag="IWO35_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ0315984.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="IWO35_000910"
     CDS             complement(20737..21396)
                     /locus_tag="IWO35_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0315985.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IWO35_000911"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IWO35_000911"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ0315986.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="IWO35_000912"
     CDS             complement(22169..23035)
                     /locus_tag="IWO35_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0315987.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="IWO35_000913"
     CDS             23144..23785
                     /locus_tag="IWO35_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ0315988.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IWO35_000914"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IWO35_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ0315989.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="IWO35_000915"
     CDS             complement(25226..26125)
                     /locus_tag="IWO35_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0315990.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="IWO35_000916"
     CDS             complement(26127..26924)
                     /locus_tag="IWO35_000916"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ0315991.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="IWO35_000917"
     CDS             complement(26902..27606)
                     /locus_tag="IWO35_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ0315992.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="IWO35_000918"
     CDS             27737..28312
                     /locus_tag="IWO35_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ0315993.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..29107
                     /locus_tag="IWO35_000919"
     CDS             28430..29107
                     /locus_tag="IWO35_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0315994.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8565 a         6169 c         5232 g         9143 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taattaaaa
//
LOCUS       DACECZ010000021        29045 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.021, whole genome shotgun sequence.
ACCESSION   DACECZ010000021 DACECZ010000000
VERSION     DACECZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.021"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(125..1228)
                     /locus_tag="IWO35_000920"
     CDS             complement(125..1228)
                     /locus_tag="IWO35_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0315995.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(1450..1920)
                     /locus_tag="IWO35_000921"
     CDS             complement(1450..1920)
                     /locus_tag="IWO35_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ0315996.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            2177..3496
                     /locus_tag="IWO35_000922"
     CDS             2177..3496
                     /locus_tag="IWO35_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAQ0315997.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            3937..5577
                     /locus_tag="IWO35_000923"
     CDS             3937..5577
                     /locus_tag="IWO35_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ0315998.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            5731..7053
                     /locus_tag="IWO35_000924"
     CDS             5731..7053
                     /locus_tag="IWO35_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ0315999.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(7115..7546)
                     /locus_tag="IWO35_000925"
     CDS             complement(7115..7546)
                     /locus_tag="IWO35_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0316000.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(7610..8446)
                     /locus_tag="IWO35_000926"
     CDS             complement(7610..8446)
                     /locus_tag="IWO35_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ0316001.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(8636..8938)
                     /locus_tag="IWO35_000927"
     CDS             complement(8636..8938)
                     /locus_tag="IWO35_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ0316002.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(8964..9395)
                     /gene="ruvX"
                     /locus_tag="IWO35_000928"
     CDS             complement(8964..9395)
                     /gene="ruvX"
                     /locus_tag="IWO35_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ0316003.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(9392..9658)
                     /locus_tag="IWO35_000929"
     CDS             complement(9392..9658)
                     /locus_tag="IWO35_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ0316004.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(9912..10637)
                     /locus_tag="IWO35_000930"
     CDS             complement(9912..10637)
                     /locus_tag="IWO35_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ0316005.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(11003..11073)
                     /locus_tag="IWO35_000931"
     tRNA            complement(11003..11073)
                     /locus_tag="IWO35_000931"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11039..11041),aa:Gly,seq:ccc)
     gene            11190..11942
                     /locus_tag="IWO35_000932"
     CDS             11190..11942
                     /locus_tag="IWO35_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ0316006.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            11958..12437
                     /locus_tag="IWO35_000933"
     CDS             11958..12437
                     /locus_tag="IWO35_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ0316007.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(12688..14268)
                     /locus_tag="IWO35_000934"
     CDS             complement(12688..14268)
                     /locus_tag="IWO35_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ0316008.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(14261..15004)
                     /locus_tag="IWO35_000935"
     CDS             complement(14261..15004)
                     /locus_tag="IWO35_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0316009.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(15180..15956)
                     /locus_tag="IWO35_000936"
     CDS             complement(15180..15956)
                     /locus_tag="IWO35_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0316010.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(16204..17040)
                     /locus_tag="IWO35_000937"
     CDS             complement(16204..17040)
                     /locus_tag="IWO35_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ0316011.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(17051..17980)
                     /locus_tag="IWO35_000938"
     CDS             complement(17051..17980)
                     /locus_tag="IWO35_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ0316012.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            18172..18525
                     /locus_tag="IWO35_000939"
     CDS             18172..18525
                     /locus_tag="IWO35_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0316013.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(18599..19255)
                     /locus_tag="IWO35_000940"
     CDS             complement(18599..19255)
                     /locus_tag="IWO35_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0316014.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            19481..21415
                     /locus_tag="IWO35_000941"
     CDS             19481..21415
                     /locus_tag="IWO35_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0316015.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(21501..22724)
                     /locus_tag="IWO35_000942"
     CDS             complement(21501..22724)
                     /locus_tag="IWO35_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ0316016.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(22718..24616)
                     /locus_tag="IWO35_000943"
     CDS             complement(22718..24616)
                     /locus_tag="IWO35_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ0316017.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(24610..25728)
                     /locus_tag="IWO35_000944"
     CDS             complement(24610..25728)
                     /locus_tag="IWO35_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0316018.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(25811..26644)
                     /locus_tag="IWO35_000945"
     CDS             complement(25811..26644)
                     /locus_tag="IWO35_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0316019.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(26637..27554)
                     /locus_tag="IWO35_000946"
     CDS             complement(26637..27554)
                     /locus_tag="IWO35_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0316020.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(27781..28905)
                     /locus_tag="IWO35_000947"
     CDS             complement(27781..28905)
                     /locus_tag="IWO35_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0316021.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         8901 a         5840 c         4944 g         9360 t
ORIGIN      
        1 gtatgaaaaa atatttcaat tttatttttt cgtttcaaat tattctatta gtgacatcct
       61 tgaactataa aaataggagt acggcatcat ctgccgcact cctatttttt tcgtcatttg
      121 tttcttaaat aaaacgattc tcacgcaatt ttattttttt gatatcttct actgtttgtg
      181 ttccagctaa ttgcatgacc atttccaatt ctgttttaaa gaaatcaaac acttgatgga
      241 ctcctgtact tcctcctaaa gacaatccat aaataacggg tctgccaata gcaacaagat
      301 ctgcacctga tgcgattgct ttgaaaacat gctgtccgcg tcgtacaccg ctgtcaaaca
      361 cgattggtac acgtttatct actgcttctg ccacatattg caacgaatca aacgctgctg
      421 gaccaccatc tagttgacgg ccaccatgat tagataccca gattccgcca gcaccagatt
      481 ctaatgaacg atagacatct tcttctgact ggacaccttt cacatagact ggcaaatcag
      541 aatgagcagc aataaattct acatcttttg gactaagctt ctgtttggaa gatttgtaca
      601 cagcatccat cgtttggcct actcctgatt gataagcctg aacgatcggc attggcagtg
      661 ggaaggtaaa cccatttctg cggtctgttt cacggtttcc gccaactgtt gcatccgctg
      721 tcaatacgat tgcttttgct ccattacgct tggccacttc aagaatatcc aaattgatcc
      781 catcatcttt gctcatataa aattgaaacc attgcggtgc tttctccccg cctgcttctc
      841 ttatctcttc taatgtacaa gaagcgtaag aacttgctgt atagatcgtc ccaaaatctg
      901 ctacaccttt tgcagaagct ttctccgcct tcacatgagc taagccatga gcagcaactg
      961 gtgccatgat gattggtgca gtcagcgttt ctttgtcaaa atgtgtagtt gtatcgggta
     1021 attctacatc tcttaataca tgaggaataa tcagctgatg attgaatgcc cgttcatttt
     1081 cttgataagt aaataagtcg ccagcaccac tgctgatgta accataacca ccagctggaa
     1141 tcacttgtga agcggctatt tctaaatctt ccatattgat aaaatccact attccttcat
     1201 ttgtacctgc ttgatacgtt ttttccattt caatgccttc cctctataaa aaattgaatg
     1261 aataatatga tagcgtttac acactttata ttactcttcc gtattgatac aggcaaataa
     1321 tctgtttaca tttcagtcat tccagttgtt actactctga aagaaatgtt tagaacacaa
     1381 atagataaaa aacgatggga ttttgacaaa agtcgtgtca aagtcccttt tacgaaatac
     1441 tgtggatgcc tattgaaata agtgcgggtt gtttctttgt atttcaatcg tttcttgcca
     1501 ttcttcgttt aattttggat taccgttttc ttccttgaac gcataaggtc ccaatccaaa
     1561 aagctcagtt agtatttgat tcacgattcc atatagctcg gtgtgatcgg acacatgatt
     1621 attgaagatc atctcattat caccacgttt gaatctcaca ttcgggctct catgatgaac
     1681 ctctatgcag ttttcccctt tttttaactg acggatttct gaaaacatct cataaatatc
     1741 ttctccaata aaaccttcag caagcgccag actgtccaca agaccgccta ctgtcaactc
     1801 agagattatc gctttgttgg caccaagctt taatgtatag cttgttgctt ctactacgcc
     1861 gcatgcattg caaccacctt ctgatagttc gctttttctg atcaattgaa cttctttcat
     1921 tttctgttcc tcctcttagt tttctactta tgtatttttt atcacctgac gtaagtgtat
     1981 tttatcgtca cgatcacgtg atttattctt cagagattct acctgaataa ttcatacttt
     2041 taattaaaac catgtgaaac tgcctcacgg cagcttacaa ttactttttc atcttcaccc
     2101 gttaagtgat gaaaaaagaa cccctttctg ctatggttaa agtgactaaa ccaaccaaaa
     2161 gaaaggagtt cttttcatga ctagcttaca caaaaaccaa gtaaaattca attcaaatat
     2221 cactatttct catacaggtg gtcaattatc gagtgattcg ggtctcgtcc tagtgaaaga
     2281 gctgatgaac atctttggtt tttctgaact ggctaagcaa cacattcaca ttgaagacga
     2341 acgagcgtat tttactcatg acaacttatc catacttgag cagttcatta tgcaattaat
     2401 tgctggttac tcagctgatt ctgcagctaa tctcctgaga caagatcctg tgtttaaagc
     2461 tgttttagat aggaaagaac tcgcttctca atcttcactt tcccgatttt tagatcgact
     2521 atctgaggag aatatccatg aacttcaagc tttgaaccaa gaacttatcg ataaagcacg
     2581 cctcatccgt aatgatacgg aattaatcat tgatttagat tcgacccatt cagatacatt
     2641 tggtcaccaa gaacaaacga attataatac acactaccaa acctatggtt accatccatt
     2701 ggtagctttt gacggattga ctggtgactt cttaaaagct gaactacgtt caggaaatca
     2761 atatacatca aaaggcgtaa aggagtttct cacaccttta ttagagcact ataatcactc
     2821 tctaccaaac actgacatct tggttcgtgg agacagcggg ttcgctacac ctggtgtgta
     2881 tgattcgtgt gaatcaaaaa agagtcatta tgttattcga ctgaagaata atcgtagact
     2941 aggtcaaata gctgagaaat cagtacttta tggcgataat caaaagtggg aagaacgaga
     3001 agttcagtac ttctcgacca cttatcaagc acaatcctgg tcacaaagtc gtcgcgtgtg
     3061 tatacgctca acacgtgaag cgggcgaact actctttcga catgaattta tcgtgacgaa
     3121 tctatcagaa aatgtttctc ctgataccat cttttctatc tatgccaaac gtggcacaat
     3181 ggagaacttc attaaagaag cgaaagctgg cttttacttt gacaaaacag acagtcctcg
     3241 ctttttggag aatcatgtcc gaatgatgct cagcttgata gcttacaact tagtcaactt
     3301 cttaagaacg attggctttg aagaagtcca aaagggaatg accattcatt ctattcgatt
     3361 gaagtttctg aaagttgctg ggaaattagt ccaaacgggt agacgagtct atctcaaatt
     3421 atctagttat catgtgtatc agaatgaatt ctacagggtc tttgctcgcc tgaggcgagc
     3481 cagtcaatgg atctaatcca acaatctaac gattttttta ctgggaagtt atccagggag
     3541 aagtatgctc aaaatgcctt acacccaaaa tttattcctc aatatttttg aacagtgaat
     3601 gtttgaagta actttttagt caaaatcagt tgatagggat attagtgata catatttcaa
     3661 taaaatgtgc caaagaatta aagctatgaa ttattcagga ttctatttat ctatctaaca
     3721 tatcctttat tcttctactt ttctttcttt agcatataag agatacgcat agtaatcaag
     3781 caaggattgt tgatataaaa agaacaaata ccgctatttt gatttctttt gactctatta
     3841 ctcatataaa taaaatttat tatacctgct ctttttgact atatcctcat ctgactccta
     3901 tacttcgttt gtattaaaac ttaggaggaa tcattcatgc ttacagggct aaacttatta
     3961 aataatcctt ttctaaataa aggaacagct tttacaaaag aagaacgggc aaaatacgga
     4021 atcactggta tgcttccaag tactgtccaa acattggaac aacaatctgt ccaagcttac
     4081 ggtcaatact taagcaaaca gaccgatttg gaaaaacgta tcttcttaat gaatctattc
     4141 aatacgaacc ggacattgtt ttataaattg atgggcgaac atttagttga attcatgcct
     4201 gtcgtttatg atcctgtcgt tgcggattca atcgaacaat acaacgaaat tttcttgaaa
     4261 ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa atatcaaagc aagtttgaaa
     4321 aacgcagcag atggacgcga cattcgtttg atcgttgtga cagatgcaga aggaatctta
     4381 ggtatgggtg actggggtgt gaacggtgtt gacatcgcta tcggtaaatt gatggtctac
     4441 actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg tatcaatcga cgctggtact
     4501 aataacgaag aattattgaa taatccatta tacttaggga atcgtcatgc tcgtgtagaa
     4561 ggcgaaactt actacgaatt cattgatcaa ttcgttcaat cagctactga attattccca
     4621 gaattgttat tacattggga agattttgga cgaggaaatg ctgcaacgat cttagaaaaa
     4681 tacgaagata aaatcactac tttcaatgat gatatccaag gcacaggaat cgttgtacta
     4741 gctggtgtat taggtggatt gaatatatca ggtgagcaat tgaaagatca aacgatcttg
     4801 acatttggtg ctggtactgc tggtgtaggt atcgcaaaca tccttttaga tgaaatgatc
     4861 cgtcaaggtg tacctgaaga agaagcacgc caacatttct atcaagtaga taaacagggt
     4921 ttattgtttg aagatacaaa aggattgact cctggtcaaa tcccattcgc tcgtaaacgc
     4981 tcagaattca ctaatgctga tgaattgaca aacttagaag cagttgtaaa agcaatccat
     5041 ccaacgatca tgatcggtac ttctacccaa cctggagcat tcactgaaga aatcgtgaaa
     5101 gaaatggcgg ctcatacccc acgtccaatc atcatgcctt tgtctaatcc aacaaaattg
     5161 gcagaagcaa aagcaaaaga tttgattgaa tggacggacg gaaaagcttt agttggaaca
     5221 ggtatccctg ctgctgatgt ggaatataac ggtgtaactt accaaatcgg tcaagcaaat
     5281 aatgcgttga tgtacccagg tttagggctt ggtttgattg cttcaacagc tacacgagta
     5341 aatgctgaaa tcatttctca agctagtcga gcattaggcg gaatcgttga tgtaacaaaa
     5401 ccaggagcag ctatcttgcc tccagtagca aaaatcactg aattctctca aacgattgct
     5461 gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc gtgaagaaat cacggatatc
     5521 aaagaagctg ttgaatctgc taaatgggtt cctgaatata aatctttaga agattaagat
     5581 tgttatagta aaagaacata cagaaacttt gtatgacgtc taaaaagaaa aaagcgactg
     5641 cggcaaaaca gcagccagcc aactactgct cttttgccgc ttctctttgg cttttttcat
     5701 acgatacaga ataagaaagg atctgtaaaa atggcacaaa aaacaacaac cgcaactgca
     5761 gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa ttaccggcgt aagtttgccc
     5821 ctttatttga tcatggtcct tgtcctgatc attgcaatgg ctttaggaaa attaccgacg
     5881 aatatgattg gcccaatcgc tgttttggtc attttcggta atttatttca ttttatcgga
     5941 acaaaaatcc ctatagtcaa aagttattta ggtggtggct ccgtttttgc gatctttgcc
     6001 tctgccgcga ttgctacatt tgggatctta cctgagtatg tagtaaaatc cacagaaaac
     6061 tttgtcagca atatgggctt tttagacttt tatattgctg ccttgattac tggtagcatc
     6121 cttggtatga accgtaatct attgatgaaa gcttctgttc gttttattcc cgttgcactt
     6181 atttcaatgg tcgtctcctt ttttgccgta ggactcgtag gtatgcttat cggaaatggt
     6241 tttgcaaatt ccgttttata tatctcattg cccgcaatgg ctggtggtgt tggagccgga
     6301 gctgtacctt tatcaacgat ttatgcaaat gttttaggta cggacgcttc ttctatcatt
     6361 tcccgcctga ttcctgcgac agcaatgaca aatgtcctag ctatcattgg tgcagcgtta
     6421 gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta atggtaaatt aatggaaaaa
     6481 ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt ccattccaca attaggtgtc
     6541 ggtttgatgg tatcattgac tttcttcatc ttaggacaaa tctgtaacta ttttgttcct
     6601 aaagtccatg cttacgcatt catgatcatt atcgttgtta tctgtaaaat cgcaaaccta
     6661 ttaccagaat attacgaaga tgcagctatc atgttcaaca acttgattgt caaaaacttg
     6721 acagcagctg ttcttgccgg tatcggtatt gcgttattga atctaaatgt attggcttct
     6781 gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg ttatcgcgat ttcagtagtt
     6841 tcaggatttg tcggtcgttt gtttggttta tatccaatcg aatcaactat tactgctggc
     6901 ttatgcaaca actcgatggg tggtacagga aatgtagctg tcttatctgc agctaatcgg
     6961 atggaactta ttgcttttgc tcaaatgggc aatcggcttg gtggtgccat cgttttgatc
     7021 atctcaggtt tcttgatgca attactttct tagtcaaaaa aaatacctga tcatcaagta
     7081 tcggcttgat gaccaggtat tttttgataa aaatttacag gattttcaaa aatctttttt
     7141 gcattttatc ggaatgataa ccgatgcgtt cataaaatcg gtgtgcttct actctgtatt
     7201 ctgcagaatt aagtcttatt gctgatattc ctcgtctttt cgcttcttct acgccagtca
     7261 tcaaggcctt tccgattcct cttttttcag catgtttaga cacagcaagt gccataatat
     7321 tgaacatcgg atcgctgaaa atcgattcat aggactccgc ttgtacatag ccagctgctt
     7381 ttttagaagt aggatcttca tagaccaaga aaaaattttt ttctggctcc ttcaacagtt
     7441 tcttcatttg tctcaccgta acggttaatg gtacatcata tcctaattgt tccgcattga
     7501 tttctcgtag tgcttctgca tctcctaaac gcatttcccg caacatccaa atcaccagcc
     7561 aatttttatt tttttaaatg aattttttta tcacagtata ctcctttttt cacttaaaaa
     7621 aagacttttc agtgattttg acttgcttga atccagatcc ttctaatgtt tgcaagatga
     7681 cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac aatcgcttga aaaataaagg
     7741 tcactttttt tggagacaaa accctgctaa atcttgctcc ccacaaacct ccaataacag
     7801 cagcggggat aacataaaac aacatagata aatcaaagcg cataaagcta cttgttaaag
     7861 caatcgttac caattttgcc agttgagaaa agaaaatcgt agatagagaa tatagtgttg
     7921 cttcttttat tggcaatgca aacatcagca tcaacaaaga aacattgact ggtccaccac
     7981 caatacctaa aaaactagct aaaaagccca gaaccccccc acatataagg taccaagtcc
     8041 atgacgtcaa atgaaatttt ggctgatgat gcttggtgta gaagaacgca aaaaccaagg
     8101 tcaataccgt caaaaaaatt tggatcagct gcacatgctt ctcgttttca aataactgta
     8161 aaaaaacttc gaatacaatg ttgccgacta ctcctccaac aacagctcca ctagataccc
     8221 aaccgacaat ctgccaattc agtgatttcc cgcttgatac ttgtcgcaca gtcgaaacaa
     8281 tggacatcgt gaataccgca actgttgagt aaaaagaaat ccctgcaaca gaatgggcgc
     8341 cgataagatc aagaatcggt ttgatcaaca cgcctccacc catcccggaa atagcgccaa
     8401 ctgtgttcgc cagaacgatc accacaaaat aaatcagacc aatcatcttg tatttccttt
     8461 ctcttgttta tatcttttag cgattgttac tcttttcttt tattatacag taaaagaaac
     8521 aaacgaggct tcgaaagctt ttctactttt taaggaaatt tagtgctaaa cgaaaaaacc
     8581 gggctctact tttcagagag tccagctttt cttacacctt atcgattctt ttgttctatt
     8641 cttcttccgc catgaatgta ttgaatactt cttcgatcat atcccattct gcgtctgttt
     8701 cgatttgttc taactcgcct tctgttccat cttcgttttc gatgtatgca taagcttgta
     8761 attctacatc ttcatcttct gaaacaccag ctggatataa aagaacatag ttacgtccaa
     8821 attcttcttg tccgtcaact gttagcaaga tttcgtataa tgtttcattt ccttggtcat
     8881 ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga atggttatgt tctgtcataa
     8941 gattacctct tttcttttta ttattaaaat aattgcttac ctgagccgtc taaataattt
     9001 tgtaaaatca tcacagctgc taatttatcg atgacttttt ttcgtttcgc tcttgaagta
     9061 tttgcctgct ctactagcat acgttctgcc tgaacagtgg ttagtcgttc atcttgataa
     9121 tcaaccggca aggagaatag ttctttgatc ttctctccat atgacatgga agcttctgcc
     9181 cttggtccga tggaattgtt catattctta ggtaatccaa ctacaaaacg gtctacttcg
     9241 tactgcgcca ctaattcctt caaccgatca aaaccaaact cttcttctgc ctcattgatt
     9301 cgaatgattt ctactccttg agctgtccag ccaaagggat cgctcacagc aacgcctact
     9361 gtcttagaac cgacatccaa gcccattact ctcataaatt cactccatga tttgagaggt
     9421 agtctttgac aattacttcc atgatttcat cgcgttcgtg acggcgaatc aaatttcgtg
     9481 catcacgata acgaggaatg taggccgggt ctccagaaag caagtagccg acaatctgat
     9541 tgattggatt gtagcctttt tcttccaaag cccgataaac aatcgctaat gtttcgctca
     9601 cttctttttt gcgactatcg tcaaaaccaa aacgtacggt ttcatctgta aaacccatga
     9661 ttctacacct ctttcccgca aggtaattcc agcttacctt ctacaccctt attttactcg
     9721 aagaaaggga aaactacaaa caaatattcc aaacttctga ataaattaag aaacacgatt
     9781 tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc ctctttccat aaagtatacg
     9841 ctatatttct ttgaaagtat gtttttacac aattttttca taaagttcat aaaaaattcg
     9901 aattgctgct actaattttt cgttttcgtt gcttgccctg tctcataatt gatggtgtag
     9961 ccatcttgaa cattgtagat aaagacattg aattcaattt ttttgtcttc aatgctttct
    10021 gcttccaact gcacacctct tgctaccagt tcatttccct cgaaatgagg agtgacacga
    10081 tagcgtacat gatgatttgt ttccttgata tatgccgcaa tatcattttc gtgggcaagc
    10141 atgtacggag cattcaacga acgtgtccct gtcattaagt ttttaatgtt attattttga
    10201 ccagtcaact ggaaaccaat caaatggctg cggttgtata accattgtcc gtcacttaat
    10261 ttcttttgtt tccaacctgt cggatcaata tacaaaggtt ctctcttttc agtaggaaac
    10321 aagtcttttc ccaacatcgc attggcttgt cctactctgt tcaatttgtc tatatttgaa
    10381 tagctttccc atgtcccttt ggaaagacta agatcctctt ttgtaaactc cggctgattc
    10441 ccattgattt ctatttcttg atgttttcca tcatattctg gcacatccgc tacttgagaa
    10501 gcttgcggtg tggaatcaaa aatggttgta atttgttgga agatatctga atctttttgt
    10561 gtggtggaaa taccgagtgc ccctgcaatc acagcagcga cgatccccac gatcgccttg
    10621 gttgtttttt tgctcatttc tttactcctt tactaacagt tccctaaatc ttaacatatg
    10681 ttcccctata aataaatact ggaaagaaat agaaacagta aaaaaataaa accagttttt
    10741 agacggctta agaatacaaa aaaagagacg gattcatcgt ctctactcaa tcacaaaaca
    10801 ctatttacaa ggtatttatt atcattcaaa aaatttttct taatactcaa aactaaacgg
    10861 cttctgtacg acctcttggt aaacagtgtt caaaagcttg catctgcggt aataagtcca
    10921 accaagtcaa aaacataaga agcttttttg cttgactatt cttattactt gatgcagggc
    10981 agctttttca cagcctcttt taagcgggtg acgggaatcg aacccgcgac atcagcttgg
    11041 gaagctggga ttttaccact aaactacacc cgcgatacaa aaacagtata cgcctctttc
    11101 actccttcct caagtttttt tattctttac gcttccgata tgttttgtag taaaataaaa
    11161 ctaatcaatc aattattgga gaagatgcta tgacgggaaa agaattagat ctgttgctgg
    11221 acacttgttt actcgctggg aaaatcatga tggaaagcaa tgcggagatg tatcgggtag
    11281 aagatacgat gagtcgaatt gctttagctt ctggaaattt ccgtttggtc agctatgtca
    11341 ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac catccgaatg gaacaaatta
    11401 ccaaccgttc gattaattta gaaaaagtag taaaagtaaa tgatctttca cgtaaatatg
    11461 ttgcttcaga gctaacatta gaagaacttt actgcgaact gcaagcaatc gaccgagacc
    11521 gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc agtgatcagt ggaacaatta
    11581 tggtgttatt tggcggaaag atgaccgatt tacctgtgac tttagtcatt ggaggattgg
    11641 gatacgcagc ttatctatat agtttgaaat tttttagaat caaattttta tcggaatttt
    11701 tatcttcttt attgattggc atagccgcta tctacagcgt acgttttggc ttaggaagca
    11761 atcaggatct aatcattatc ggatgtgtga tgcctttagt tcctggagtt caaattacga
    11821 acgctatccg tgatttgcta gctggacatt atgtctcagg agtttctcgc ggaacagaag
    11881 cgatgatgac tgctacgatg atcggttttg cgattgcttt tattttccag cttttttatt
    11941 aggaaaggaa cacaagtatg gggacgatat ttattcagtt ttcttttagt tttttagcga
    12001 ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc attgatttgt tgtggtcttt
    12061 ctggtgcttt tggctggatg atctactgga tctgtgttca aataggcgga tctgctgctt
    12121 ttggttcatt gcttggttct ttaggagtag cattcatcag cgatcttttc tctaaacgtt
    12181 tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt tcctctagtc cctggtggtt
    12241 tagcttatca agcggttcga aatctcgtta ctgaatccta tcgagaagcg gctcattatg
    12301 cggtccaagc aattatgatt gctggagcaa tcgctttagg tcttgtatta tcagaagtct
    12361 ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc aatcatgatg aaacgaaaaa
    12421 aacggctttt taaataaacg aagtgtaaca aagaaaaggt tgtgaaagag acgttcagcc
    12481 ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt aaccgcagga gcaataagaa
    12541 gtaattatga gtattaaaaa aagttttgta aaaatagctt ctcacatttt tacataaaat
    12601 tgacttaata ctgagaaact gacttttgaa caccctctat ctgtaaaaaa ggggctgcga
    12661 catgattttt gtcacggccc ctcttcttta ttcaaatttt ttccaaaacg ttcgttgata
    12721 ataaacgatc catagagcgg aaagtattag aactaaaccg atcacaggaa tattcactaa
    12781 ccaaaatgga taaaacaaag ccaacattcc gtataagacg ataagaataa tactgatgat
    12841 caatgaaccc atcatagttg cgactaaacc caaacttcct gaacctctgt taaataattg
    12901 gttaatattg gtccagttca gcagcagcaa ccgataatct ctagcgaaat aacgtagtga
    12961 caataagtag cagccgaata aagaaccaaa tagcaagcta gggataaaaa acaaaggcag
    13021 ctgaaaagac agtccgctta ataatgcgat cccaccagtt aaaaaactct gcaatatcca
    13081 tccgaatctg aacttttctt ttaaatattg gttcatagag attggcaaag aacggataaa
    13141 cagaaaattt tcttgatcca aagaaataag attgctgata aaagaagtct gattaactgt
    13201 taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg attaacttat tatctaaatc
    13261 agttaatttt atttcgcctc ctagggcaaa agcaagaata aaaatgattg gagttaacaa
    13321 ggaagaagaa agaacttgca taatcaaatt aggttctttt actaattgag cattgtaact
    13381 aaataaaagc tgatgcacat tttggttctt tttatgggtt ctttttattt tgaactcact
    13441 gtttgaatca gtcagttgct ctgtaagctt tggtactatc agctgtttta cagcaaggaa
    13501 aaatcccaga gccgtgatag ctagtattcc ccaacccaat aaaccttgtt gagaaagtgg
    13561 ctgactggca gcatcataga atggcaataa tatcgaaatc ggatgccgat ctgttaactc
    13621 attttcaata gtccccgttt gatgattcat ccacatgatt ccagcaaatg cgatcagcat
    13681 cgagctgcca agcaagatac tagaaactgt ttttctatgt tttttaaaca ctgctgttcg
    13741 agccaaacca aaaacaatca aactagccag acaaaaaaga ataatcaaaa atagagaaaa
    13801 caaaaggatt gagataatga tagtaagcaa aatccctaga tcagctcgcc atccggttaa
    13861 aataaacatg actagaagcg gaaaaacaaa aggaatgatg gtcaaagcaa ctaccaaaat
    13921 ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc aaaaatactt gcaggtcatt
    13981 tccttcaaaa aagatattat aaatgactga tatactttga gttaaaccta acataccaaa
    14041 aagtgccacg taataagtaa agaaacctgg caatttaaca aagttcatag caaacatagc
    14101 aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga ttttgagaca aaagatagat
    14161 agttaatcgc tttccctttt ttcctttctc tcttgcttta ttcgtgagct gtggattaac
    14221 atataaaaga ttaactcgaa ttaattctaa taattgtcgc ttatacatac ggtccatctc
    14281 ctgtcgcttg ccttccagcc attttcaaat aaatcttttc tagcgtttca tcaggtgagc
    14341 cattcaataa ttcactaact gccccctgat aaatcaattc acctttcttc aaaatagcca
    14401 gtttatcgca taactgttgt gcggtatcca atacatgtgt agaaaaaatg actgtttttc
    14461 cttttgcggc atgagccttc atcatttctt tcaaatcaaa tgctgcttga ggatcaagtc
    14521 cttgcaaagg ttcatccaat acccaaatat ctggatctgg cagcaacgtg ccgatcaaaa
    14581 tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt ttcattttga tgactataca
    14641 tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt tttttgtgtg tctaattcat
    14701 aggcagctga aatcaagtcc caatattctc cagccgtcaa ttgtaaaaaa atatctgggg
    14761 aatcaggcac ataaccgatt ttttgtttga tactcaatcg attttccgtc aatttcattc
    14821 catctactaa aatcgttccc gatgatggtt caataatact aactaaactt ttaatcgtag
    14881 ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat ttccccttgt cttatcgaga
    14941 gagacaagtc ttttaatgct tgttttttcc cgtaactttt acttacattg atgaattcaa
    15001 tcatttctgt actccttttg tttgttatta ctttttattg tacccttcgt tattggtgga
    15061 gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa cgactaggct gctacttggg
    15121 ttgttctctt ctacaggaaa acactcttct aacaggtaag atatgagtct ttgttttctt
    15181 taaaatgatt cattttctat cttttcttgc ataaaattct ttagtttatc aatacttctc
    15241 tttcccatct ctagttgttc tccatttgca aatgttacta tttcgtttac tctgctaata
    15301 gatgtaatgt tttcagtatt aatgatataa gcttttaaat ctttacataa atactcatat
    15361 tcgagtgatt ttttatattt tttaattgtg ccgtttacga gaatctcttc atctttcaat
    15421 actaggccta ttttattttt ataacctggc atgctcatta gataaaggat ttcatcgtaa
    15481 tggatatagt gatcgctatt accataacgg acagttgtcc accttttttt cgtagcatgt
    15541 tgaacaggat agttttcttt ttctttcaat aagtaacttt ggatgctttt tttatttttt
    15601 tcataagtga attcttttat caaataacca ctaggcgtaa tctttcgatt aataacagtt
    15661 attgctttat tcggatgaga cgttagataa acaattttac acttttcatt cttttctcta
    15721 agcatttcgc ctagttcgat accgctataa taggcgttca aatcgatgtc taggaaaaaa
    15781 atatctgagg aatagatttc attattttta atgaattcaa caaattgtat aacgttcaat
    15841 ccctgaacga tctcaaattt ccaaggactt aataaaattc tggattcttc caaaatttct
    15901 actagcattt tttcaaaaac caaactatca tccaaaataa atatacgcgt taccataaaa
    15961 agtacctcca ctcgatattt tactatcaaa atcatttcat acaacttttc aacatacatg
    16021 ttaggaataa attgtaattc tcttcacaat cttttccaac acctttgaaa ccgatatcaa
    16081 atactttcag gatgctgctt ctaaaataaa tatcaaacaa atgaatacat catgcaaaaa
    16141 aagccagaca accgacgtcc ttgcgtgtca gctgtctgag ctatgtttat ttattttgtt
    16201 cttttaaaaa gaacgataca catactgatt tttcggagca gagactttct cagcgtttgt
    16261 aacaactaca gatgggattt cacgcttgaa tggttgataa tagtctgatt ggatggttcc
    16321 ttctatttta taccattcat catttttaac cgttactcct tccggcatat gcgtcaacaa
    16381 accaaagact cctgaatctg ccacacaatg gataatgccg aatcggaaca agaagatatc
    16441 tgtattgcta tcgttcggtg tttggtaaac aaatccttca tagctgattt ttttgccaat
    16501 gaattcgcta gggtaattat aaattagctc cataacttct aaatagtttt catctgtgat
    16561 cttgatagtt tgcttatccc catacttttc tttcaactca ttcatttggt ctaagtagtc
    16621 tgatttgttg aaataaatac tggtatccgg ctttaagtat tgtgtctgca tctccggatc
    16681 ccctactgat tctttactta ctgggaaatt aaatcctttg gcttctacga tcgtagtgtc
    16741 gagactaact gttggaaaca acgtccccac gattactggc aaagctagca gtacataagc
    16801 gattccacgt tgataaggtt ttgacaaccc atggtcatga tcgtgctcgt gtgccttttc
    16861 ttctgctttt ccgtttttga cccacaaata cagctgaact aaagccaaaa gagcagataa
    16921 aatcattgaa agaatagcta agtagcgata atggacattg atatattgat cgagtttgcc
    16981 agaaacttgc aagtacatca tcaattcgaa atacccagat aaaatcaaaa accgcagcat
    17041 attttccctc ctaaataaat aatgtgaatc caataacgct aagggcaatc agtccgatca
    17101 gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat cagtagattt ttgatatcga
    17161 ccattgggcc aaagaccaaa aaggcagcta caggtgctgt gccgaacaga cttaacaaag
    17221 aagatcctat aaacgcatct gcttctgagc aaagagacat cgtaaaggcc aataacagca
    17281 tgatgataat ggctaaaatc tttgttgagc cgagcgttag cataattcta gttggtaaat
    17341 aagtctgcat cgcagctgcg attaatccgc caatgatcaa ataacgacct gtatcaaaaa
    17401 attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg gatcagctga ttttttggtt
    17461 cattcttctc actttgttct tcttcatgga catgatgatg gtgattttct gctactttag
    17521 acgtgatttt ttctttcaat acagcgtctt tttgaaaata ggcaagccag acaccaatca
    17581 caagggcaac aaaaaagctg cctatcaccc gccagcttgc catctgccaa gaattgccaa
    17641 atgcgataaa agtggaaaaa atcacgattg gattgatgat cggagcagtc agcataaaag
    17701 caaacgctgt gtgaactggt acgccttttt taacaaattg atgaacgatc ggtacgatgc
    17761 cgcattcaca tgaaggaaag aaaaatccta agatcgaacc agtaaaaata gagaagatac
    17821 gattttcagg aagccatctc ttcacacgtt ctggggtcaa aaatacttgt aaggcgcctg
    17881 agataagaca gcctaataat acgaaaggta atgcttcaat aacgatcgat aagaaaatcg
    17941 tactcatctg caaaaccgaa tttggaataa aagaaaacat gagtttcctc ctttttattt
    18001 ctagttcatt cgctttgcaa atatacgctt ttattcaaaa aaaacaagtt gctctgtaaa
    18061 tttttacaca actcttattt tttaccaaag cacttcaatt gactttttct gttattccca
    18121 ttaaattaag gtctcataca tatcacttct atcataaaag ggagaataaa aatgaaacaa
    18181 accactaact tgactgaagt tcaagacata ctgcaacagt catccgttgc cgtcatttat
    18241 ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc cacgactaga ggaacttctc
    18301 acacattatg atattccggc acttcatttg gatgcatttg aaacaccaga agtagctagt
    18361 aaatttgaag tgctgactgc gccagttgtc ctgatttttc atcaaggaaa agaagtcttt
    18421 cggcaagcac ggttcatcga ctttaaaaaa atccgttatc tactagatga actgacagca
    18481 gaagatgatt ccctttctta tgaagaaatc ttccgaacaa aataatcgtt gtccaaacag
    18541 aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg ttcaatcgct ttttattttt
    18601 attctgctgt attttcggaa tgcgcatttg cgtcagaatg caagaaatag atcaaggttt
    18661 gcaattcatt cgtcaaatca acattttgga tcaatacatc tgacggagca ttcaatcgag
    18721 cagcggtaaa gttcaagatt cctttgattc ctgcttccgt taactgattg actacttctt
    18781 gtgctctttc agcgggtatc gttaaaattg ctacttcgat ttgttggatc ttgatttgtt
    18841 ctgacatatc ttccattgga taaacgggga taccgtctac gatgcgccca acgatttcct
    18901 cattcacatc aaaagcacaa ctgatacgta tgctattact ttgatggaat ttgtatttta
    18961 gtaaagcact tcccaagttc cctacaccga tcagcgcaac atttgtcaat tgatcgtcgt
    19021 ttaatgtctt tgcaaaaaag tgcataagat cttctacatc ataaccatac ccacgttttc
    19081 ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc actatctact tgaacggctt
    19141 cacttaattc agttgaagac acttttttct ttccagcatt atttaaaatc cttaaatagc
    19201 gataatacaa aggcagtctt ttagctgtcg cctttggaat aatatgatct ttcacgaaat
    19261 gacctccacg tattgtctaa ttacatcttt gatttctcca tcatagcagt atttatagga
    19321 aaaaatcaat tccattgctc agatattgtg aaattttatt ctttattcat ttatttatca
    19381 tttctgcaaa tctcgcagta taatggaatt tatggtagac tactaattga aaaatagttt
    19441 tttatttgag gagaattcac aaagtagaaa agaggattcc atgatattat tacaagccaa
    19501 tcagattgct cgccattttg gtgcagaagt cttatttgaa aacatacatt tagaaatcag
    19561 tacaggcgct cgtgtcgctt tagttggcag aaatggcgct ggaaaatcga ctttattaaa
    19621 gatcattgct ggaattgaag cacctgatga aggaaagatc gctaaaaata aaacagcgac
    19681 tctcggatac ctcgctcagg acacaggact tgattccaat gaaacagttt ggaacgagat
    19741 gctgaaagct tttgaacaag ttagacaaat ggaagagcgg atgcgcgagc tagagatttt
    19801 cattagcgaa ggtacgccag acacacctgc ttatagttct tatttaaaag aatacgaccg
    19861 gctgcaacac gatttttcag atcgaaacgg ctatggttat gaaaatgaaa ttcgttccgt
    19921 attgcatggg tttaaattcg attcttcatt ttatgatcag aaaatcgata ctttatcagg
    19981 cggacagcgc acacgtttag ctttagctcg catgcttttg caacaccctg acattttgat
    20041 tctagacgaa ccaacgaacc atttagatat cgagacactt tcctggcttg agaattatct
    20101 gcaaagctat actggtgcat tattgattgt ttcccatgac cgttattttt tagataaagt
    20161 agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc cattacaaag gtaactattc
    20221 gaaatatcta gatttgaaag cagaacagtt agcaagtgaa tggaaggctt atgaaaagca
    20281 gcaaacagaa atccataaat tagaagactt cgttgctcgg aaccttgttc gcgcctctac
    20341 cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa atggaccggc tggatcgtcc
    20401 tcaaggggat gaaaaacaag caaactttct attcagtata gaaaagcctt ctggaaatgt
    20461 agtcttgcaa atcgaagatg ccgctatcgg atatgataat gtcaccttat ctgaaccaat
    20521 caatctagat atccgtcgtc aagaagcgat tgctctagta ggtccgaatg gtatcgggaa
    20581 atcgacgttg ctgaaatcaa tcattcacca gatccctttt atccgtggaa aagaaacgct
    20641 agggacaaat gtcaccatcg gctattacga tcaaggtcaa gctgatctgc atacaaacaa
    20701 gacgatccta gcagaattgt gggatgagca tcctaccact ccagaaaaag agatccgaag
    20761 tgctttaggt gctttcttat tttctggcga agatgtagaa aaaccaatcc ctctgctaag
    20821 tggcggtgaa aaagcacggg ttgctttagc caagttagcg atgaacaaag aaaatttttt
    20881 gattttggat gaaccaacaa accatttaga tattgacaac aaagaagtat tagaaaatgc
    20941 attgatcgat tatgacggca cactgctttt tgtttcccat gaccgatatt tcatcaatcg
    21001 gatcgcgaca aaagtcgtcg aactctctga aaatggcagc aaagtctatc taggagacta
    21061 tgattattat ttagagaaaa agcaggaaga agaagaaatc gccgaattgt tggcacaaga
    21121 tgagccagaa tctgctcctg ctcccacgaa caaatctgat tattaccaaa gcaaagaaca
    21181 gcaaaagctt atccgtagcc ttcaacgaaa aattactcag gttgaagaag aaatggcgcg
    21241 gatcgatgaa tggatcgatc agttggaaca agaaatggtc gcgccagaaa acctggaaga
    21301 tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct gctagacaag atcaagaaac
    21361 aaaattgacg gaatgggaag aactaagctt ggaattagaa gaatatgaaa attaacgaaa
    21421 aaaccaaaac agaaaccatt catgctgttt tggtttttct ttttttattt tatcaaacaa
    21481 aaattccata tttacctttt tcaccaataa gaagaccaat tactagtcat tctcactaaa
    21541 gcttctaaat aatagtaatc accccaaata caaggtgtat ttaccccatt atttttgggc
    21601 ttattataaa cagactgtgt taaaaagcca tcttcttctt ccgttgtagc atatctttct
    21661 ttcaattgtc gcataatttt ccaaacagca gactggtata agttcttttc tgttttgtct
    21721 aatagacctg attctattaa ttctaataat ccacaaacca tgatcaccat actagatgta
    21781 tctttttctt ctctgctaga atcatcaaag atcaaatccc aataacaaat ggtatcttct
    21841 ggcaaccgat tcataaaata attggctaat attttgctta aatcaaaata tttttgttct
    21901 ttcaaataac gataacttaa tgcaaaaccg taaatccccc atgcttggcc tctagcccaa
    21961 caggaattat cactataacc ttgatgcgta cttccatatc ttggttttcc atctttgaca
    22021 tccatataaa atgtatgata agttgaacca tcttctctta ctaaataatc tgctgcattt
    22081 tgaatatgag cctgagcagc ttttttatat ttttgatttc cagtttcttc actcgcccaa
    22141 tacaataatg gaagattcat gcaacaatcc atgatcattc taccttgttg tgaaggatca
    22201 ctaggatctc cccaagcttg tataactcct gccttctccc aaaaacgctg aagtaatgtt
    22261 tctgcagcat caattgaagc ttccttcatt tttcgatttc ccgttatttt ataatctgct
    22321 acggttgata aagaatacaa aaaaccaaga tcgtgatgat tagtgataat ttgacagtca
    22381 atcctgtgga taaaatctat atctgctttt tcagctgttt gttgaaataa aggatcaccc
    22441 gtcttctcaa ataacaacca attaattcct gtccaaaatc cttctgtcca accaatattg
    22501 tctcttaatg gataacgtcc ttttatagga cactcgcttg ggaattgata ctgactgtta
    22561 ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac gctcgatctc attattccat
    22621 tctttttcca ttaattcttt ataacattca tttatccatt tttctggatg tattatctct
    22681 tcaaacatca ctttatcaag catttcccca ccagccttta gcataatttt agtctttttt
    22741 tattgttttt attttggata gttagcccgg ttgtcccatc aaaatcaatt ttgggacgat
    22801 tgttccatac ttctaaacta taatcccgat cttgatgagc aaccactcct gttccgagcc
    22861 aatgggttcc ttttccaaat gtaccctcta atccaggaat aaaagtgttt tcccatgtca
    22921 tcaagtttgt attgggcaaa cccataatag ctgtggattt tcgtttaata gtatcgtcac
    22981 ttaaacagat aatacctgaa aagccagctt cactcaccct atagttttcc ttgtcttctt
    23041 ctatgattat tccttctttc tcaggagaac attcaatagc aaaccctgtc tcatatagaa
    23101 catattctgt atctaattct actttgtgta cgcgaatctg ccagccttca aaaggaataa
    23161 gccaagttct gatttttgcc tgtaaagtag gttcccatgt agaggcaagt acctctttct
    23221 tacatacaat gtctcgtaca ttatttcgaa cgatgaactg tccataggtt ttgatgctct
    23281 cattcttaaa actggtcgtg atatatgtgt ccgctcgctg aatccctagc attgaatcca
    23341 ttgccatttc tgctttcaat tgattcgctc gcggtatcga aaaaccaaat tgactagaat
    23401 aagcaaattt taaatatttt tcattggtca atgtatggaa atttggccct aattgtcccg
    23461 cattcaaaaa aaatgtatgt ccattatcat gttgtatgag caaattggct tctgaaagaa
    23521 gtttagttcc ttcacgaata ggcataggca tttctttagc cgtccaatac tcatggtctt
    23581 caggtagcgc caacaataaa aaaatcttat tcagccataa tggagataac gtactattat
    23641 agggttcagt caatgtcaat tgagggtagg tataaccaat attcaggata cctttttcat
    23701 caaatatttc tttttgcatc caccatctta tattccgatt gactaacccc ttgatttcac
    23761 ctaatttaaa aggcaaaaga tcggcccaca acatagctga ccaaaagctt gaaactgcaa
    23821 aacgataaat catgcttcga ccaaaaggca catttgcacc atcttccgca aaaaaatgaa
    23881 tatagtctct agaaaaatca atagctcggt tcttatatat ctggctaatg ggcttttctg
    23941 gttctagtac gctataaatc aaaccataat aatgaaatgc aaaaggatta taataatctt
    24001 ggcgacctct tgaactgtct cgataccagc cattttgaag ataacaattt tctatattat
    24061 caaatgcttc tttcagtgct gattctttaa ctggataacc aaatttttgg ataattgttc
    24121 cgacaattat cttaaaaaat tgccaattac catctgcaca ttcatattca aaaatttgcg
    24181 agaaccaatg aataatctgg cttttttctc ctgtatctaa ttttttccat aataattgtt
    24241 catgatgata taacgctaat aacattgcgg gcatttctac catacgctgg tctctttcat
    24301 gtatttctcc ccaataatcg ggatgggaag gatctgttcc agcttttatt cctgatagaa
    24361 ttaatttcaa ccatcgttca tctaattggt caattgcggg ccccgctccc cataaaaccc
    24421 gagagaatcc ttccattcct gcaattttat ttccatatcc taccccttga tgtatataat
    24481 caatttgtgc gccacttgct cgataaaacg gctggagtgg ttgatttatc tttttaaata
    24541 agtcaaagta atcctgtctg gtccttaata gtggtactgg atgtttcatt gtattttctc
    24601 ctctttttac taccaaatag ctaattccga ctgttctaat cgaagtaagc cttcaataaa
    24661 aaaataatca gaatagatca gtggcacatt ataagtcagt tctctatggt atgcttctgc
    24721 tccgcctcca atgatcccat cttcatccat attccaattt gcatagtgct tttctgtctt
    24781 ttggagtata ttgatagcag cttcatgata catttttgat cgtacagagg aaacatatcc
    24841 tgctaattcc agaagaccgc atgcagcgca tactccagca ctggtatcaa tattcgaatc
    24901 ttttacagca aaatcaatga gaggaatata accagttttt tcgattttct gaataaaatt
    24961 tactgctatc ttttctgcta tagctagata gtacggattt tttgtgtaac gataacttaa
    25021 cgcgaatcca tataaagccc atgcatttcc tctgctccaa caagaattct cattgtatcc
    25081 ttgcccgcca agtaaccgaa tatatttccc tgattctgca tcaaattctg cgatatgccc
    25141 ggttgatcca tctttcctca ctagatactt agctgttgta tctgcatgct gccgagcaat
    25201 tttagagaac ctcggatcac cagtttccct cgaagcccaa tacagtaatg gtaaattcat
    25261 catactatca ataatcatcc aaccaggttt atggtcattc catgctacta aatagttccc
    25321 tgaaatatta tagcgtccag caagtaccgt agctgcatgt aaccctagtt gtcgactatg
    25381 tttgttttga gactgttgat aatgtgcaat agccgatggt aagaataaaa aacctacatc
    25441 atgatgaacg tctgaaaaat ggtacagggc agattctaat aattgttctt gttgtattgc
    25501 tgcctctttg aacttttctc gttttgtctt gtgaaagagc tgccataaaa taccactcca
    25561 aaaaccattg gtccaccagt cgattcctct gtcctttatt acgtctgtat attttccgtt
    25621 gtaagaaata tatggaattt tattccccat tctgtctaat tccgcatcta tcttttcaga
    25681 agctacattc aaaatttttc ttgaccattc cttcgtctgt tcattcatgg atatcccgcc
    25741 attcttttaa tttttatgaa tgtggagttg ctcctagaac gaactagatg gttaacattc
    25801 gttatttttt ttacttattt gcagctccaa tgtcattctc cttaaattta gactgcgccc
    25861 acacataaat caagaccatt gggatcactg aagttgctaa aattagcata attgcgccat
    25921 aattaattgg ctcacctgta ctagaaaccg tcatattttt gataattaag ggaaccgtat
    25981 atttatcttt atctgaaagc atgactaatg gaaatagata ttgattccaa cttcccataa
    26041 agataagtaa accactagta ataatcgtag gtgataaaat cggtacagcg attttcgtaa
    26101 aaatcaccca ctcattggca ccatcgattc tggcagcctc catcagtgct gtaggaaatt
    26161 gactgctata ttgtcgcatc ataaacactg aagttgtact ggcaatcgaa gggatgatca
    26221 cacctgcata agtattcgat aatcctaaac gactcataat gtaaaataaa ggaatcaatg
    26281 tggtaaaaaa aggcaccatc ctagatagca taactgtatt gaacaacagt ttattcccca
    26341 taaaacgata ctttgctaat gcataacccg ccattgtaga actggctgta gctgaaactg
    26401 tactgattac agcaataaca atcgaattcc aaataatcaa gggataatta tatctcgcca
    26461 tgatggcttg ccagtttttt aatagcatat ccccaaactt gaaattgatt ccgccactta
    26521 aaatttcttg attactttgc gttgaagcta caatcatata taaatatggc atcaaaaata
    26581 ttaaggcacc aaacacaata atgacgtacg taaaaattct gctttgtatc ttttgtttag
    26641 tcatttttat cacctccgaa tttaaattga atagccgata atataacaga tacaaacacc
    26701 atcgtccagg caatagcaga ggcttttcca aaatcaacca tttcaaaact tgttttatat
    26761 aaattgactc ctacgaccaa agctgaattt tctggtccat tggaagtccc aaagaatata
    26821 tttgggatat tgaaattacc caagccattg atagtggcta aaactgtggc cattaaaatg
    26881 attggcttta gcatggggat cgttaataaa aagaattgcc gaaatttcga tgctccatct
    26941 atttcagcac tttcgtatat ctctttagaa atcccttgaa gaccagctaa aaataaaata
    27001 gtgttatatc ctacactaag ccaaatcatc gtgatgatca ttgatattcg tatccaaaaa
    27061 ggatttgtta accactgtac accgttaatt cctagcgcat tcaaaaatcc attgaataac
    27121 ccagtcggat tgaaaaaagt catgaatacg gaagctacag ccaccgttga tgtaatggtt
    27181 ggaatataat agacagttct aaaaaatcct tttcctttta actgtgctga attcaataaa
    27241 acagcaaagt aaatagctaa tagcatcgtt attggaattg atcctgccat gataatcaaa
    27301 acattcgtgt aagatttaat aaacaatgga tctcggaaga tagctagata attgtcaaat
    27361 ccaacaaatt caaatgagta gccaattttc ataaaactaa aagcaaacga gataatgatg
    27421 ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg tgaccaatcc ataagcactt
    27481 tttttatgag acgtatctgt tgcttttttc ttgctgttct ttattgtctt gcttggtaac
    27541 gaagattgca tcatcgttaa gtcctcctaa tattttttaa tcaccagtta attttacgcc
    27601 gtacttatga gcaaaatttt ctgcttcttt tagatatgaa ttttcaaaat cacctgtttg
    27661 ccaatagtta tatgcagcgg tagtcaaata agacgtagca tctgaataag tggctggtaa
    27721 aacagtattt gatgtatttt cattgatttt attcaatagt tcatagtatt tttgtcctcc
    27781 ttagtagtca aactttttct cagctgcttc tgtttgatag gccaatgagt tagatgtagc
    27841 cacgcccagc tccagtgcgt tggctaagca ttcttcatct gtcagcataa atgtaagaaa
    27901 ttgttgtgcc acttgtggat ttttggcatc tttagggaca taaaaacttg atcctcctga
    27961 tactgcacct tttcctggat tttcttttga aaaaggaatc agtggagcca tgcgccattt
    28021 gtcagatgca tcaggaaaat tcaaagacat attcgtagca taccagccgc cagcaacaaa
    28081 cattgcagat tcttgaaatg ctttctcagc atcttgtgca ccgtagaaat gtacaatatc
    28141 tgagtcgatc atttctttga ttaactttgc agcatgtttc gcttctttag tattcagacg
    28201 gatatttcct tcttcatcaa gcaaagattg attctgctgg gctagcatgt tgataaacaa
    28261 ttcttggtcc ccagattctg gcaaggcaat tccaaaacta ccagtttttt cttttaatgc
    28321 ttttgccacc tggatatatt cttcccaaga actgatttct tcaaaatcta cacctatttc
    28381 agcaaatttt tttggttgat aataacaaag tgctggggta aaatcactag cccaaggaat
    28441 aatcgtttca ttactcgttt gttgacgcaa aatattcaat ttttgatcat aaaactgatc
    28501 gccatatttg tcataaaatc catattcatc agcactataa aaagcatctt gaaatttttc
    28561 cagcacacct gctgctgaag tgtctgccat ggagataata tccggcatat cttcgtgtgt
    28621 taccaatttt ggggtgatca tttgtactgt tgctgttccc gaagctacat tcacaatttt
    28681 taagtcaata tttgctccag ttttgcggtt atagattttt gcagcatttt caacatagtt
    28741 tgtcagtttg ccgtttgcga catatacttc taatgtagtg gcttttttta tcattggatc
    28801 tgcgatgttt gctgttactt cttcgttagt ttttccacat gcacttaagc tgaataatag
    28861 ggtaatgttt agcaaagcta tcaccaattt gtatcttttt ttcatgtcta tcccttcttt
    28921 cttttcaagt tcttatataa aacgctttca cttatgtata ttaacggaat tagtaacaca
    28981 aatatttata taaaaaaata ttttacttgt cgttttggct tttcattttt gttataatag
    29041 tcatt
//
LOCUS       DACECZ010000022        28443 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.022, whole genome shotgun sequence.
ACCESSION   DACECZ010000022 DACECZ010000000
VERSION     DACECZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.022"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..195)
                     /locus_tag="IWO35_000948"
     CDS             complement(<1..195)
                     /locus_tag="IWO35_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0316022.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYY"
     gene            702..1961
                     /gene="lysA"
                     /locus_tag="IWO35_000949"
     CDS             702..1961
                     /gene="lysA"
                     /locus_tag="IWO35_000949"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ0316023.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1948..2748
                     /gene="dapF"
                     /locus_tag="IWO35_000950"
     CDS             1948..2748
                     /gene="dapF"
                     /locus_tag="IWO35_000950"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ0316024.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2751..3632
                     /locus_tag="IWO35_000951"
     CDS             2751..3632
                     /locus_tag="IWO35_000951"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ0316025.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3629..4345
                     /locus_tag="IWO35_000952"
     CDS             3629..4345
                     /locus_tag="IWO35_000952"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ0316026.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            4351..5046
                     /gene="dapD"
                     /locus_tag="IWO35_000953"
     CDS             4351..5046
                     /gene="dapD"
                     /locus_tag="IWO35_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ0316027.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            5062..6063
                     /locus_tag="IWO35_000954"
     CDS             5062..6063
                     /locus_tag="IWO35_000954"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ0316028.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            6060..7421
                     /locus_tag="IWO35_000955"
     CDS             6060..7421
                     /locus_tag="IWO35_000955"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ0316029.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            7381..8532
                     /locus_tag="IWO35_000956"
     CDS             7381..8532
                     /locus_tag="IWO35_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ0316030.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            8507..9676
                     /locus_tag="IWO35_000957"
     CDS             8507..9676
                     /locus_tag="IWO35_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0316031.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9851..11230
                     /locus_tag="IWO35_000958"
     CDS             9851..11230
                     /locus_tag="IWO35_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0316032.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            11511..12308
                     /locus_tag="IWO35_000959"
                     /pseudo
     CDS             11511..12308
                     /locus_tag="IWO35_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            12497..13165
                     /locus_tag="IWO35_000960"
     CDS             12497..13165
                     /locus_tag="IWO35_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ0316033.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(13365..13751)
                     /locus_tag="IWO35_000961"
     CDS             complement(13365..13751)
                     /locus_tag="IWO35_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316034.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(13723..15105)
                     /locus_tag="IWO35_000962"
     CDS             complement(13723..15105)
                     /locus_tag="IWO35_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0316035.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(15261..16448)
                     /locus_tag="IWO35_000963"
     CDS             complement(15261..16448)
                     /locus_tag="IWO35_000963"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ0316036.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(16455..17486)
                     /locus_tag="IWO35_000964"
     CDS             complement(16455..17486)
                     /locus_tag="IWO35_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0316037.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(17502..18956)
                     /locus_tag="IWO35_000965"
     CDS             complement(17502..18956)
                     /locus_tag="IWO35_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0316038.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            19131..20036
                     /locus_tag="IWO35_000966"
     CDS             19131..20036
                     /locus_tag="IWO35_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0316039.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            20127..20804
                     /locus_tag="IWO35_000967"
     CDS             20127..20804
                     /locus_tag="IWO35_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0316040.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            21085..21981
                     /locus_tag="IWO35_000968"
     CDS             21085..21981
                     /locus_tag="IWO35_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0316041.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(22036..23406)
                     /locus_tag="IWO35_000969"
     CDS             complement(22036..23406)
                     /locus_tag="IWO35_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0316042.1"
                     /translation="MEEKKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            23644..24390
                     /locus_tag="IWO35_000970"
     CDS             23644..24390
                     /locus_tag="IWO35_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0316043.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            24387..25226
                     /locus_tag="IWO35_000971"
     CDS             24387..25226
                     /locus_tag="IWO35_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0316044.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            25227..26177
                     /locus_tag="IWO35_000972"
     CDS             25227..26177
                     /locus_tag="IWO35_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0316045.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            26318..27070
                     /locus_tag="IWO35_000973"
     CDS             26318..27070
                     /locus_tag="IWO35_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316046.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            27089..28240
                     /gene="queG"
                     /locus_tag="IWO35_000974"
     CDS             27089..28240
                     /gene="queG"
                     /locus_tag="IWO35_000974"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ0316047.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         9338 a         5131 c         5931 g         8043 t
ORIGIN      
        1 ataatagcca tttcgttgac tttgtcggtt ttctgttcgt tcatattctt ttgcttgaat
       61 atattctgtt cgttgatttt ccattagttg attaaatacc gttgttaaaa tatttttaga
      121 aacgtcatcc tttacagaat attcaataat gctttgaatc tcttcgcttt tcagtgtaaa
      181 atgtacttgg gtcatgtaaa agtcctcctg ggtatgtttt tgtcgttaaa aacattgtac
      241 cgtaaaagga ctgttacatg gcctttttac ttttacacaa ttataaggac tttatctttt
      301 ttgtggaatc agcaaattga aaaataaaaa agtatttgat tttatttata ttttgaaaga
      361 tgaatggcaa aagctagaga aagaagaaac tgcacttatt tatgaagaaa ttgaaatgat
      421 tcataaaatt gaaaatatcc gaactattcc gaactatatt gaagcgaaaa tattttttca
      481 tcactaaata gtgttgaatt tatgttttta tgtataaata gcttctaata gttcgggtat
      541 tttgttttta taaccatttt tattcttatt tttaaaaaaa tatttcgaaa tatactagac
      601 aattcagaaa attttgttta atattattcc aataaaattc aaatctttat caaatgaaat
      661 tccaataaaa acggaaaaaa agtgaaaaga ggaatgaaaa aatgacagaa ttgataattg
      721 gcggagtcac agcaagcaca ttggcagaaa agtataagac acctctgtat gtgtatgatg
      781 aagaaaagat gagacaaacg atgctgtcat tcaagcgagg ctttgtatcg aaacgttttg
      841 atacaaaagt gctttacgcc tctaaagcat tccaaacgat tgagatgctg catttagttc
      901 gtgaatatgg attcgggcta gatgttgtca gtggtgggga aatctatacc gctttgcaag
      961 ctgattttcc tagagatact atctacttcc atggtaacaa caagacgcca gaggaactga
     1021 tctatgcact tgaaaatgat ttgttgcata ttgttgccga taatttgatg gaagttgaat
     1081 tattagcaga attaagccaa cgctatttaa aaaaaatctg tatcatgctt cggttgaatg
     1141 tcggagtaga agcacatact catgaatata tcgtcactgc gcatattgat tcaaaattcg
     1201 gcatgagcta tgaaagtaaa gagtgccaac aagcccttca gttgataaaa gaaagcagct
     1261 atctagagtt agaaggattt catgctcata tcgggtcaca aatttttgat gttactgctt
     1321 ggttagctga aatcgacaaa ttgacgaatt acttaaccga ttttgaccaa cctctttcct
     1381 tgaatcttgg cggagggttt gggattcgtt atacacaaaa tgatcagccg atcccagtcg
     1441 aagaggcact aaaaaaactg attgaatata cggaagaagc tttggaaaaa cgaaaactga
     1501 ccattcgtca attactgatc gaaccgggta gaagtattgt tggagaagct ggtacaacac
     1561 tatacacgat tggtttcatc aaaaagacac cacataaaaa atattacttt gtagatggcg
     1621 ggatgacaga taatatccgt ccagctctgt atcaggcaga atacgattgt gatttagcag
     1681 caaaactgtc ggaagaaaaa acagagaaag tgacgattgc aggaaaaatg tgtgaatcag
     1741 gcgatgtggt aattaaagaa gcttatttgc cgaaagcaga atcgggtgat cttttagctg
     1801 tttattccac gggagcttat ggatactcga tgagcagcaa ctacaatcga gcgacacgac
     1861 cagcagttgt ttttgtcaat aatggccaat caagactgat tgttcgcaga caagcatttt
     1921 ctgatctact aaggggggaa gtttcctatg atccttcata aatataatgg ctgcggaaat
     1981 gattttatct tattagatta cgcagaagac acagattata gtcaattagc cgcttcttta
     2041 tgcacaaaag aaaaatatga tacagacgga ttgatcgccg taaaaatcga tccgttggaa
     2101 atgatctatt acaacaaaga tggcagccgc gcgccgatgt gcggaaatgg cattcgctgt
     2161 tttgctcgat acgtaaatga tcagaacatg atgaatgaac aggaattcaa cgtagagaca
     2221 ttagcaggca caagaaaaat tacggtgttg gatccctcac ctttttattg cagcgtagat
     2281 atgggagtgc cagatgattc tccggaaaaa atcaaagcta atcaaactta tccaataaaa
     2341 aaccaggaaa tagagatcaa tggtgaaaaa gtaacgatca ctagtttatt tttaggaacg
     2401 attcataccg ttgtttttgt tgaaaatgcg cgtgaggaag tctggagaaa aagaggtgaa
     2461 ctgatctgtc atcatccttt gttcaaagaa aaaaccaatg tcaactttgt agaagtagtc
     2521 aatgaacaag aattgatcgt ccgtacctat gaaagaggag taggttggac acttgcttgc
     2581 ggtacaggtt gctgtgcctc ttatgtggtt gctaaggaaa ctgggaaaat ttcagcagat
     2641 caggtagttg ttcacttgga acaaggagac ttgatgatct ctggtgagga tgcgatacaa
     2701 atggctggcc cagcagttca tgaatggaca agaatattgg aggaatgaag atgataaaag
     2761 gttcgatcgt ggcgctgatc acgccgatga atgaagaggg atcagtagat tatgctggtt
     2821 tggaaaaatt gatccaattc catttagatg aacaaacaga tggattatta gttttaggta
     2881 caacaggaga atcttctacg ctcacacaat ctgaggagga gcagatcctt caactgacag
     2941 taaagaaagt ggctggccgt gtgccggtga ttgcaggagc tggaacgaat aatacgaaag
     3001 aaacgattga aaaagcgaag cattttgctt ctttgggggc agacgcactt ttggtcatca
     3061 ccccttacta caacaaaaca agtgatgcag gactagctgc tcattttaca gcaattgcag
     3121 aagcctcacc gattccgctt attttgtata atgttccttc tagaacaggc atgtcgattc
     3181 caatccatgt attagtaaat ttagcagaac atcctaatat cattggtttg aaagaagcct
     3241 caggagatat ggcttatgtg atggatgccg ctcggttgat tggagaagaa ttcttccttt
     3301 acagtggaaa tgatgatttg atcctaccag taatgagtgt aggaggaagc ggtgtaataa
     3361 gtgtatgggc gaatatccaa cctaaaatag tccatgaatt agtaaaagat acgcaggacg
     3421 gaagatggca gcaagcaaaa gaaaagcaat taaatgcact tgaactgatc catgccttgt
     3481 tcagtgagac caatcctatt cctgtcaaag cagccatgtc attattagac ttaccttccg
     3541 gacctctacg cctgccttta gtttctcttt caaaagagaa aaagaaacaa ttagctcagc
     3601 ttttgttaaa agaaaggacg gttgaaaaat gaaaatcgga ctgattggca gtggacagat
     3661 gggaaataga gtcgaagaag ttgcacaaga aagaggagat acggtccttc ttttacggac
     3721 ggcacctaaa gaagcaacta cgcttgcttt tacggaactg ccagaactgc ttattgattt
     3781 ttctcatcca gataatttag agaaaatcct ttcgtatagc ttatcatatc aacttccgtt
     3841 agtcattggc acgacaggtt atacacaatc acagttccaa gaaataaaag aacatgccaa
     3901 acatgtacct gtgatgtata gtgctaattt ttcgttcggc atcatggtga tgaatcaact
     3961 aatcaagcaa gcagctgcaa tgttgcaagg ctggcagatc gaactgattg aaaaacatca
     4021 tagtcgtaaa aaagatgcac catctggtac tgcaaatatg ctgcttcaaa caatccaaga
     4081 agtgcaaaag ctgcagcccg tatacaactg gcaagggaaa aaaagagaag aaaaccaaat
     4141 tggtgtacac agtattcgtg caggttcttt acctggtgaa catgatgtat tgtttgctgc
     4201 tactgatgaa atatttacga tcaaacatga atctttttct aatcgaatct ttgctgcagg
     4261 cgcagtagaa gcagcggact ggttgaaaga tcagtcacca ggttattaca aattagaaga
     4321 tttattaatg gacaaagggg tgtaaaaaac gtggaagctc aagaaatcat ccgctacatt
     4381 caaacggcat ctaaaaagac gcctgtgaaa gtctatctga atgtaaccga gccaatcgaa
     4441 tttaaagaaa gtaaagtttt tggtgaagga agcagcttcg ttgtttttgg tgactatgag
     4501 acgattgctc cggttttaga agctgaatca gaaaaaatca ttgaaatcga aatcgaaact
     4561 gctgcaagat acagcgcagt tcctttgcta gatattaaaa aaattaatgc tcgtatcgaa
     4621 ccaggtgcta ttatcagaga tcaagtgtca atcggcaaca atgccgtgat catgatggga
     4681 gcgattatca atattggtgc agtaatcggt gaaaacacga tgattgatat gggtgctgtc
     4741 ttaggtggac gagcgacagt gggcaaaaac tgccatattg gtgcaggagc tgtcttagca
     4801 ggtgtgatcg aaccagcatc ggccaaaccg gttatcgtag aagacggcgt actggtaggt
     4861 gcgaatgcag tgattgttga aggtgtccat atcggaaaag atgcagtcgt ggcagcagga
     4921 gcagtggttt tagaagatgt ggcagcagaa actgttgtcg gtggaatacc tgcacgtgta
     4981 ttaaaaataa gtgatgatca gacaaaagaa aatacagcat tgattgctgc gttacgcgaa
     5041 ttatagagga gatggagaaa aatgcgcgaa atagatacct gtattgtggg ggcaaccgga
     5101 ttagtcggcc aaacgatgct caaagtgtta gaagaatatg attttccagt aggacgattg
     5161 aaattattag cttcaaaacg ttcagccggt aaagtttgtg tattcaagaa aaagaaacac
     5221 actgtacagg aattgacaga aacttcattt gaaggatatg atttagcgct attttctgca
     5281 ggtgcttctg tatccaaaga gtttgcgcca atcgcacgaa gtcagggact atttgtcata
     5341 gataattcct cagcatggcg ggaagattcg gatattccat taatcgttcc agaggtcaat
     5401 ctggaagact atacattgaa tcggctgatt gcaaatccaa attgttcaac cattcaagcg
     5461 gtacttccgc ttaaagcttt acaagaagcc tttggtatcc agcgagtcaa ttattcgacc
     5521 tatcaagcag tttctggttc cgggcaaaaa ggcattgaag atcttcatcg tacgcaaaat
     5581 gacgaggaac cagttttgta tccttataat attagccaaa cggtgattcc ggaaatcgat
     5641 cagcctttgg aaaatgggta tacaaaagaa gaacagaaaa tgataaatga gacccgtaag
     5701 attctgcatc agcctgcttt gccagtatca gcaacctgcg tacgtgtgcc aatcgaaaat
     5761 ggacatggag tctctgtggc agttcaactg caaaaagagt ttacgacgga agaaattaga
     5821 gagtgtctca gccagtttcc agggattcaa ttagaagatg atctagcagc acataaatat
     5881 ccgacgagca ttcttgctag aggaacagat atggtttatg ttgggaggat cagaaaagac
     5941 acttctttag agaacggatt gttgttctat acgactgccg ataacatacg caaaggagcc
     6001 gctgcgaatg ccgtacagat tgctgcagct ttaatgcaga aaatagcgga ggtggcaaga
     6061 tgagtaatac gctgaaagta gcgaaattcg gcggaacatc gatgtctcat gaagagcaat
     6121 tcaaaaaagt ccgtacaatt gttctttcag atcccaatcg ccaagtgatc gttgtttctg
     6181 ctttaggcaa acgtacgtca gatgatgaca aagtaacaga cttactttat ttgatttatg
     6241 cttaccttca gcatcatgtt gattggacac ccctttggaa aaggatttgt gatcgtttca
     6301 ttcaagtaag agatgttctt gccctgcagt tacctattga agatgaacta gcacgcatac
     6361 agaaacaact ggaagacgaa aagataactg aagattattt agtaagtaga ggtgaatatc
     6421 tgactgctca actgatggct gagtatcttg gttaccagtt tattgacgca aaagacctga
     6481 ttttctttga ctatgatggg aaaatcgatc aggaaaaaac agcacaggaa atcacaaaag
     6541 catttaaaag atatccgaaa atggttgttc cagggtttta cggggcgaac cctgcaggaa
     6601 aagaaaaatt attgggccgt gggggaagcg acattaccgg cgcgatactg gcgaagatgt
     6661 tgcaagcgga aagttatgag aattggacgg atgtttcagg aatcatgatg gctgatccgc
     6721 gaatcatcga tcatcctaaa atcatcgagg aaatctcttt tagagaactg agagagatgg
     6781 cttatatggg ggctaacgtt cttcatgaag cagctgtttt cccagttcaa gaggctgata
     6841 tccccatcca aataaaaaat accaatgatc ctttcgctca agggacaaaa atatcgaatc
     6901 acgaaataga aaaggaaaat ggactgacag gaatagctgg acgcaaggat tttctttcca
     6961 tcactttatt caaacgtcat atgtccgatg aaatcgggtt tatctggaaa gcaatgagca
     7021 tttttgctaa acatgagata tcaatcgaac atattccttc agggatcgac aatatcggag
     7081 tgatcgtctc agcagaagcg attgccgata agttatttct catcacgaaa gaattaaaag
     7141 aaacacttgg cgtggaagaa atcgtcgtga tcgaagactt agctttgatc tctgtcgttg
     7201 ggggtccgca taaagaattg attggattat ctgggaaagt gttatcgata ttgaacaaat
     7261 tagaaattcg cacatcgatc ttatcacaag gagcacagga gttgaatctg atcatcggtg
     7321 taccgaataa tcaatatgaa acagtagtga agggtatcta tgaagggatg gtgaatacgg
     7381 atgctgaaag aatatcgaat ggcactccat caaattcctg aaatcgggtt tcatgaattc
     7441 aagacaaaaa aatatttata tgaacaagta aaaaacagcg gggccgttat ccatgaaatc
     7501 ggcgaaacag gactactcct ttattttgat aatcagcaag aagaaacgat tgcttttcga
     7561 acagatatcg atgcactgcc agtcaaagaa gaaacgaatc tctcttttgc ttctaagcat
     7621 gaaggttata tgcatgcatg tggtcatgac ggacatatgg cgatgctttt agggttgact
     7681 gactttataa ctaaacacat tgaggatctt atttataatg tagtactgat ctatcagcca
     7741 tcagaagaaa tttccggtgg cgcacaaagc gttattcagt caggattgtt ggataaatat
     7801 gaagtacaag caatatttgg ctttcatcta tggcctggac tagctaaggg agaagttttc
     7861 tctcgaccag gacctttgat ggcacaaagc agtgaaacag acattatcat taaaggccag
     7921 tctgcacata ttgcttctag tgaaaaagga atcgatagtc tggaggcagc tgtacgattt
     7981 ttgaagaatg tttatgattt tgaccggcaa ttaccggcag agtggaatca tctaatgaaa
     8041 tttggccaaa tccaaggtgg aacgatccga aatgttttag ctggagaagt tattgtatcc
     8101 ggaagtatcc gttcttacag ttcagatatg caaaattatt tgaagagggg attagaccat
     8161 ttagcagaac agtttcactc tacagctact atttcttttc gctataatga cggctatcca
     8221 gctgtaatca accatagagg cttatatgca gcactgttag attcaaacaa actacatgaa
     8281 ttagatgctc cagttcttca agccgaagat tttggcatat atacgcaaca atatccatgt
     8341 gtatttttct ttttaggtgt cggtgatacc cctgctttac atgatccgac gttcgatttt
     8401 gatatggatg ttttagaaaa tggtgtagaa tggtacaaaa cgattctttt tacagaaaat
     8461 cttcttgtaa aaatcgatag cagttcaaaa gaggagcgag taaataatga tgaatttatc
     8521 caatcgattt agtcctaggc tttctagaat agaagtatca aaaatacgac aattcgatca
     8581 gcaaatatcg tcgataccag atgtgatcaa actcaccctg ggagaaccgg attttccaac
     8641 tcctgaacat gtcaagcaag caggaatagc agcgattgaa gaagactttt ctcactatac
     8701 cggaatgaga ggattagagg aactgcggga agcggcttgt atatttcagc aacagcgtta
     8761 tggattaact tatgatccgc aaacggaagt tttgacgaca gttggtgcaa cggaagcgat
     8821 tgcctctgcg ttgttatctg ttttggaaga aggagacaag gttcttattc cagcgcctgc
     8881 ttattcagga tatcagccat tagtggaact agctggtgct gaattgatcc cgatagatac
     8941 ttctgacaca gggtttgtct gtcagccgga acaatttgaa cgagcatttg aacaatatgg
     9001 atcagctgtc aaagcagtca ttttaaacta tcctaacaat cctactggga cgacattgtc
     9061 tgcaaaacaa ttgaaagcaa tcgctgaggt tttaaaaaaa tatccggtat ttgttataag
     9121 tgatgaagta tatgctgaat tgacttatag tggaacgcat atgtccatag ctacttatct
     9181 tccagaacaa acaatcgtga taagcgggtt atccaagtca catgcgatga ctggttggcg
     9241 agtgggcttt atttttgctc aaaaagccct tattgacgaa ctaattaaag tccatcaata
     9301 tctggtgact gccgctacaa cgatgtcgca aaaagctgcg gctgaagcgc taataaatgg
     9361 ggtgaaagat agcgagaata tgaaagagca atatcaaaca cgaagagact atctggtcca
     9421 gcaattggcg cctttaggat ttgaagtgac ccagccaaac ggtgcatttt acttgttttg
     9481 caaactccca gctgccattc aaactgactc ttggcagttt tgtgtagaat tagcggaaca
     9541 ggcaaaagtt gcgtgtatcc caggaattgc atttggccca gaaggagaag gttatatccg
     9601 gatttcttat gctagcagca tggaaaaatt acatgaggcc tgcaaacgga tcaaagactt
     9661 tttaacagaa cagtgattca tagattttat caaaaaaaca atcgttaatt ttgcggtata
     9721 ttctgcaagg agacgattgt tttttaaatt tagttcaaat gaacgcgttt cttttgattc
     9781 ataactaatg taatgttaag tttaaattgt ttagaatgat tttaaaagaa tattcaagga
     9841 ggtttcatca atggaaaaaa tcaaagttgt cattgtaggt gcatctcatg gagggcatca
     9901 atcgatcttg gaactattga cacgatacgg tgaaaatgta gatatcacgc tatttgaagc
     9961 aggagactat atttccttca tgtcatgtgg tatggagctg tatttagaag acaaggtaac
    10021 agatgtaaat gatgtacgta atttcagacc agaaaacttt ccgcaatcca atgttcatat
    10081 tttaaataat cacgaagtaa aaacgatcga tgccgataaa aaaacagtaa cggttactag
    10141 cgcatcagat aacgaaacaa aagaatatcc ttatgacaaa ttgattttga gttctggtgt
    10201 caaaccaaac aatctgccag taccaggagc agatctagaa aatgtctact tgatgcgtgg
    10261 atacaattgg gcaaccaaaa tcaaagaaaa attaaatgat ccggctatta aaaaaatcgc
    10321 tgtcgtaggt gcgggttaca ttgggatcga agcagcagaa gcaagccgaa aagctgggaa
    10381 agaagtcgta ttgcttgatg tgatcgatag acctttaggc acttatttag atgctgagat
    10441 gacagatatt ttagaacaac acctaaatga aaacggtatt gaagttttga caaatgcaaa
    10501 aatcgaagct tttactggta acggaaaagt tgaggcaatc aaaacaagtg aaaaagaaat
    10561 cccagcagat ctagtcattc aagctgccgg tgtgaaggca aatacagaat ggttaaaagg
    10621 gatcgtcgac ttagatgaac gtggctggat ccaaacaaat gaatacttgc aaacgaatct
    10681 tccagatgtg tatgcagtag gagatgctac acttgcttat tctatcccag cacgcaaaaa
    10741 aatgccgatc gctttagcga ctgttgcacg tagagaagca cgttatgtag taaaacatct
    10801 ttttgaagaa gtaccaaaca aaccgttcgg cggagtcgta ggttcctctg cattaagtgt
    10861 ctttgactat cattttgcag caagcggatt gaatagcttt acagcagcaa aagcaggagt
    10921 agatgtacga gtgtcttatt atgaagatac gttacgacca aaatatgtac cagaagaaca
    10981 aggaaatcca aaagtgtcca tccaattatt ctcggatcca atgacccatc aattgctagg
    11041 aggagcagta ttatctacct atgatgttac aggtcaagga aatgtattgg cactagcgat
    11101 ccaaaacaaa ctgactttag aagaactagc agaagctgac ttcttcttcc agccaggctt
    11161 tgaccgacaa tggagcatgt tgaatctagc cgctcaacaa gcgttaggag aagaaccatt
    11221 tgtagaataa gaaaacgaaa tatttgaaaa gaggttgtga aaaagctgcc ttgcatcaag
    11281 taataagaac agccaagtaa aaacagcttc ttatgttttt aacttggctg gacttattac
    11341 cgcagatgca agcttttgaa caccgtttat tttcaaaaaa gggactgtga cacgatttgt
    11401 gtaccgaccc ccaaaagtta gaccaaaaaa tctaactttt ggaggtcggt attttttatg
    11461 gttaaatata cttttgaact taaattaaag atcgttcatg attatttgga tggcaaaggc
    11521 gattggaccg taaaaatcca gatcaagaga ttgaagcgga aatgcttaaa attcgggaga
    11581 agcacaagga ttatggctgt ttacggatga ccaatgaact aagaaaccgc ggtttctcta
    11641 tcaataaaaa gaaagttcag cgcttgatca agaagctagg aataaaagta acggcataca
    11701 cgcgtaaatc acgtcgctac aattcttatc gaggaaaagt tggagcagta gccaaaaatc
    11761 ggatccatcg tcgtttctac accagtatct gccatcagaa aatcacgacg gatacaactg
    11821 aatttaaata ccatgaagtg gatgccaaag gcattatccg tcaaaagaaa ttgtatctag
    11881 atccttttat ggatatgttc aatagtgaga ttttatccta tcgtatctct gaaaagccca
    11941 atgcattagc ggtaatggaa ggtttggagg aagctatcca gaccactaac gattgtccct
    12001 atcgtcgtac gtttcactca gatcaaggtt gggcgtatca aatgaaagct tatagaaaca
    12061 aattgaagga gtacaaaatc tttcaaagca tgtctcggaa agggaactgc ttagataatt
    12121 cacttatgga gaattttttt ggcttattaa aacaagagat ttttcacggg aaagtttata
    12181 actgtttcga ggaattaaaa tcagccatcg atagctatat ctattactac aataatgaac
    12241 ggataaaaca gaaattaaac tggcaaagtc tcgtccaatt tcgtaaaaca accgtaacgg
    12301 ttgtgtaaaa atatcatttt agagcataaa aaaaaggagt ggatttctcc actccaaaaa
    12361 agtctaactt ttgggggtca ctacagtgtc acagtccttt ttttagtaaa aggatacatt
    12421 tcttccttgt atattccatg cgtgttcgtg agaaagcata tataatttaa ttaacagata
    12481 gttaggggga gaaaaaatga ttttttcaat tacaatgaaa ctggtgttcg ggttgatcgg
    12541 tttattatta gtggtacgtt tgcttgggaa gaaatccatg tcagaaatca caccgtttga
    12601 tttagtctat acgcttgttt taggtgggat cttagaagaa tcaacctatg atgacaatgt
    12661 ccatgtaggg catgtcctat ttgccattgc gctttgggca gtaatgattt atggtatcga
    12721 acgtatcgtc caaaaaaatg aaaaggtcaa tcgttgggtc aaaggcgagc catctgttct
    12781 aatcaaagac ggcgtcatca acatgacaga gttgacaaat aaccatatcg agatggagca
    12841 gttgcgagca atcttaagac agcaagaatg cttttctctt gaaaatgcca aacatgttat
    12901 cctagagaat gccggtcaaa tgagtgtatt gaaaaaatca gatgaagaca aagctttatc
    12961 gatcctgctt gtagatgaag ggcaaattca gcataaagtg cttcagtcca atcaattgac
    13021 tgaagcatgg cttatggaga acttgaagaa agaaggctat gcagatgtaa aacagctgat
    13081 ttatgttgaa tggtcagaag aaaaagggct ctacatacta aataaagaag acgtcatgca
    13141 tgaatcttac cgaatagatg gatagataga cagaggttgt gaaatcagcg ccttggcttg
    13201 ttactcaaaa ctaagcgact ttttcacaac ctctgatatc taatatacgt tgttccagaa
    13261 gttgcttctt cgaaaaataa gccaaaattc cacaaaaatt taagaagcaa ttttcgaaaa
    13321 atcctttctt attttttgaa gttgatcact tctgtcacaa cctctcatgt cacaaaccat
    13381 ttttttgttt cctttaactc aatataccgt acgtcttccc acaatatcgg ctgatggtca
    13441 atcataaagt aatcttcata ggcttcacca atgaagtgcc cttcgacctc atccaagagt
    13501 cttccaaatt catcttttaa attgagctga atgattactg ctttacgaga taaaaaggct
    13561 tctgtcagta tactatcgat ttcttgcaat gtcatttgtt gtttaggaag aggctgtttg
    13621 cgtgcttctt cttcattttt tttgataccg ttgactagtt cttccatcgc aaaagcagtc
    13681 ccccatttta atccaaatgg ccgatctcga tactcgttat agtcaatgaa tttttttgct
    13741 gtccgtctca tcttttccac cttctaatcc actcatacca cctgcatgcc cgccaacgag
    13801 agaactacgt gcgatggcgc gtcctccttg caagagacta tgcgcatgga tgattgattg
    13861 aaagccgtat ttttttctaa tcgtatcgac aacaaaatca actttcgctt catttatctg
    13921 cttttcagga tcttcaaaaa ggttaagctg taaacctgtc gcgtatacga ggtcggaaca
    13981 gttcacgcct acacgccgaa tatcctggta gtggtaatgc tggtggaaaa tggtcaataa
    14041 agcatctgct agatctctgc tagcatttgt cgcttcgatt ttcatttgct ggtgaaaccc
    14101 ggtcttgcca tttggatcaa gatagcctaa agagtatcct acccataagc tgactacttg
    14161 tgtttttgct gcagatcgtc tcaagcgagt gccgacttgg tcggccattt ctttgataac
    14221 gatctcgatt tcttctcgtc ttgtataatc tctatctagg atttgactat ttccgatact
    14281 ttttgatttt ggtgtatatt cttgtcccaa aaaacttcga tcgatgcccc aagcatgggc
    14341 ataaagctga gtaccgatga cccctagctt gcttttcaag tgataataat ccgcgtgagc
    14401 taaatcataa acagaatgta tacccatttg tttcagacgt tttgctgttc gtttgccgat
    14461 tccccaaaag tcagtgatat ctgagatttt ccataacttt tctggtacat cttcataacg
    14521 ccattcagca aacatttgct ttccgtcttt tgagccattg tctaaggcta gtttagcaag
    14581 tagcgggtta tcgccaattc ctatagtcgt gtagattccg gtctgctcaa acacatcttt
    14641 atggatcagt ttagccatgt cgtacgcatt tgttttacca aagagtgcca tgctatttgt
    14701 tacatcaagg aagctttcat cgacggaata gacatgatgg ttcgcttcgt ctgcatattt
    14761 tttatagata gaattgattt ctctgttctt ttccatataa taggccatcc ttggggcaac
    14821 aatatataaa tcacttggat aggggaaggg aagatctctg gcacggctga tattggtgat
    14881 gccgtaagct ttttttgctg caggactgga agccaagatt aatccgctac cccgtgtttc
    14941 tgtcgaatcg gacggatagg acatgactac taatttagtt gtaaggggat caagatcctt
    15001 ttcaacgcac tctacggagg cgtaaaaaga tttgcagtca atacataaaa tatctcttga
    15061 aggttctttt tcataaacaa ataccggatt ttgattcgcc cacataacaa tcacctcttc
    15121 aacacaatta gaacacacgt tctctttccg tgtcaataaa aaacagcaat tttcaaatac
    15181 gagcaaaagg ctcacatttg gaaattgctg agaggttatt tttatctatt tgtttttagc
    15241 tgagatcgtt gattttttta tcaaacatga acagcttctg gatcgatctt tgttgtccca
    15301 tttccaatgt gtgcaacata aaattccgga gcatagccga caactttcat gtattcatca
    15361 tagacattgt ttttgaaggc aggtatattt tcttctttga caagagcaat cgcacagcca
    15421 ccaaatccag ctcctgtcat tcgtgcacct aagacacctt cttgtttttg agcagcatct
    15481 actagtgtat ctaattcgat cccagttact tcataatcat agcgaagaga gtggtgagaa
    15541 tcatttaaca gttgaccaaa tgcttctaag ttcccagctt ctaattcagc ttttgccttc
    15601 aaagtccgct gattttcaga aacggcatgg cgagctctgc gtatcaatgt atcatcgccg
    15661 atttggtcaa tagttgattc aaattcttct tcatctaagt ctcctaaaga agagataggc
    15721 aaagtttttt gcaaacgtgc caaggcttct tcgcattcgc tacggcgttc attgtatttt
    15781 gaatctgcca attcccgccg cttattcgta ttcatgatga cgattgcata accatctagt
    15841 ttcactggaa ccatttcgta tttcagcgta tttgtatcta ataagattgc atgatctagc
    15901 tcaccgaagc cgatggcaaa ctgatccatg atacctgaat tgacaccgat gaattcattt
    15961 tctacttttt tacctgtttg gactagttcc aagcgatctg tttttaaatc aaacagatct
    16021 tctaatacaa cacctactaa taattctaat gaagcgctac tggaaagtcc tgcaccatta
    16081 gggattgttc cttccactaa caactcaaac ccatgatcga ttgagtgtcc agcttctttt
    16141 aatttaagga ccattccttt tacgtaattt gcccagtttg cttttttatc atattctagc
    16201 tcatctaaag taaaactaat gacaccgata tcgtcaaagt ttgtggaata aactaatatt
    16261 ttattatcgt ctcgaggtgc tgctacacct tgcgttccat aagtgatgga agcggggaag
    16321 acatggcctc cattgtaatc tgtgtgttca ccaatcaaat tgatgcgtcc tggtgagaag
    16381 tattgtgtag tttttttagg tccgaatgtt tcggcaaatt ttgtgtttaa gcttcttttg
    16441 atttccatga tgagttattc ctttcttgtt ttgattttga attctgtcac tgtttcaaat
    16501 actgatccgg cttttaaatg aatcgaacca aatgctgaga agcgctctgc acctggagct
    16561 gtttgagtct caaatgtgat gccaccgtga tgtgccagtt ttcggttgcg catttctggt
    16621 gtttctgtgc cgaaatttgc agtgaaaata accacactag aagcatctgt agcaatacct
    16681 acttggattc ttttatcggg gctagtcaag cgagcggctt ctttacccaa gccggtttct
    16741 tttaagaaaa atggatgatc gattccgtca acaagatttt tttgatccaa atcactagca
    16801 aaaacggacg ctaatttttt aggtatttga aaatcgaaag cacttcctgc agcattttct
    16861 tttaccccta taggaatact atctgttcgc aaaggagcat aagcttcgct attgagccaa
    16921 agctcatgtt gatcgattga ctgagaagca tctcccgtca agttgaagta aacatgattt
    16981 gtcggattga ataaagtatc ttggtcactg gtgccgcgag aggtcactcg ccagatatta
    17041 tcttttgtca aagtatagcg gatttccaca tccattgtac ctggaaaacc atgttctccg
    17101 tcgggacttg tagtagtaaa aatgacagaa gcttcatttt cgccattcaa aatgacataa
    17161 gaccattttt ttaattcaaa tccaggagca ccaccatgca gattatgtcc agtttttgga
    17221 tctgtggcta attggtaagt ttttccattg agagaaaagc ggccattttc gattctccct
    17281 gcggtgcgac cgatagaagc accgatatac ggatcttttt cgatatattc ttcagcggtg
    17341 tcaaatccca aaactaattc tcgatcattt gattttaaac tgacgattct agcacctaaa
    17401 tcagagatag aaagtgctag cttgtttttg ttctccaagg ttatcaagtg ataaccttgg
    17461 ccaaaatctt tttctgtaat cttcattttc aagatccttt cttaatggga aggaactact
    17521 gcatcgtgaa gttcatcttc cattgtatca gctggagcca atttgttttc caattcctca
    17581 acgatttgtg catgtttttt ctctgtcaat gtaatcttcc aagcaaaaat aatagcggag
    17641 aaaatcatca aagcagcagg agcatagaat gcataagctt tgaaagtcgt tacgccatga
    17701 gcagtgatat cagcagcagt agcattacca gtcatcccag aagcgatagc gacaaaccct
    17761 acgatcccgt tagacaaagc accagcgatt ttatctagta aagggcgaat tgatagagta
    17821 acagcttcgt tacggatacc atttttccat tgaccatatt caacagagtc tgtgatcgtc
    17881 atcaaagcgg ataagaaaat caattgttga gggaagtaga acaatactag cccgatgata
    17941 acgattggca aacttgaacc tgcgatcgta aagaaaaagt aagaagaaat catcattgcg
    18001 atccctccaa ggtaaacgta gcgacgagag atgacgcgtg taagcattgg gaatagtgga
    18061 acagctacta aaccaagtaa ggcagcaact agaccaacga tccaaaattc agttggttgg
    18121 ccaagtacat atttgaagaa gtagaataat acggcagttg ttgctacatt ggcaatcgca
    18181 taagcgatat aagcaagggc aagccacatc aactgatcat tttgagcaac tgctttgaac
    18241 acatctttga tcccagcttt ttcattttgc tgacggatcg cagtatcgac ttcttttgtg
    18301 cccaaggcag tgataatcgc agtaccgcct gaaataacag cagcaatcac accgaaccag
    18361 aaccagcctg aagcgccttc aatatgttta tgagtaacaa gataggtaaa gaaagtaacg
    18421 atagggacaa cgactagagt agtaccattt gcacctagag aagagccgaa acgagcaaac
    18481 gtagcaaatt tttcacgttc ctttgtttcc gtactcaaag ctggaatcat tgaccaaaaa
    18541 gcgatatcct taaatgaata aaaactatcc aataagatga aaacgattgc aaataatatg
    18601 ataaataaag tcgtattatg agtagctaag ccaaagaaat tggtaaagat tgctaccaag
    18661 agtacggaac taacaacgcc gccgataact aaccatggtt tgaatttacc gtattttgta
    18721 cgagtattat cgatgatgcc accaatcaaa ggatcaaaaa taatttcaac taagcggata
    18781 cctacaacta agcttgtaac tgtagcaatc atttttgcat tcgttgcctt atctgttcct
    18841 gcgaacatct ggcttgtcac gaataccata aaataagtgc ttaatgtaac atagaatgtg
    18901 tcgtgtccaa aagcgcctaa agcgtaggac acacgttgga tcattttgct tttcataatc
    18961 tgtattcctc tcctgttcaa tatataatac taagtaaacc ggttacaacg aataacaaag
    19021 atcttttaat gacttaaact taacaagaaa tcgcttcctt tttaatatat ttctgtgact
    19081 tttatttaac tttttgttgc attatactaa aggagtaagg agggatagac gtggagcaat
    19141 cgattttttt aaaaaaggaa catcaacaag tagaaaaaga attgtatttt gatttttgtg
    19201 gtttctctaa aacgatgccg tttcattctt ttggtcctgc tattcgcaat gattacatcc
    19261 tgcatgtcgt gatggaaggc aaaggatctt atcatgtgaa aaatcaacaa ttccaattga
    19321 aaaaaggtga tctgttcttg attcgtccag gagactcgac gttttactta tcagatggag
    19381 aagatccgtg gatgtattgc tggctttcat ttggtgggag tgctgcagaa gaaatcattc
    19441 ggcagtcgct tttcagagaa gaccaataca cgatggtctc ttctgacata caaaaatata
    19501 tcgacattat tttagaatgc atgaaagata cggaagaaac cttggaaaat gaattacagt
    19561 taacagcatt aacctaccgt tttttatcag tccttttaaa agatggcgga aaggtcagcg
    19621 gtggtgtaca aaaggttcat tcctctttag ctgttgaaac agtcaaatac atcgaggcac
    19681 attatgctga aaaattgtct gttgaggaaa tcgcccagca tttagcggtc aatcggagcc
    19741 atttgtcgcg agtattcaaa aatcatatgg gtaccagtat caaggaatat ttgatcgggg
    19801 tacggatcaa tcgaggggcg tttcttcttt ctctgacgga tgaatccgtg gaaagtattg
    19861 cctatcaagt agggttcaat agtctagtcg tatttagccg aatgttcaaa aagagtaccg
    19921 gagaaacggc tacgaactat cgtaaacgta tgaaaaacga aaattttaag aatattgctt
    19981 tggatacctt gaaaaaacag ttggaaaaac aaaccgcagt ttcttggaca acctgattag
    20041 tctggttaaa atgacaagca tcggcaaaag aaacttgtgg cggttatgct acactgtaaa
    20101 gcgaagacta aaaaggagga aaagggatgc gtacacttat catcattagc catccaacga
    20161 tcgaagaatc tttaaatcag caatttttca aagaagcttc tgctggttta ccagctactt
    20221 ggcatcattt ggaatcaagt tatccagacg gaaaaataga tatagagact gaactacagc
    20281 tcttggccca gcatgatcgg atcattttcc agtttccgtt ttactggtat agttctcctg
    20341 ctcatttaaa aatttggcaa gataatgtct tggaacgagc agcacatgta ttaaaaggga
    20401 aagaactagg aatcgtattg acaacaggtg tggcggaaaa agaataccaa gctggaggaa
    20461 aagaagaata tacgatttcc gaatttttga ggccgtatca gcgaatcgca aataaattcc
    20521 atatgaccta tcttccgcca ttcgttttag cacaatttat gtatttatcc caggaaaaac
    20581 gttgggagaa gctaattgct tatcagcagt atctctcatt ggaaggcaaa ccatcgctga
    20641 ctcagcggat tgactggttt attcaaagag tacaggaaaa tcagaaaatg caggaagaag
    20701 aatctgaaaa acaagcatat atcgtagaag cactgactga ggcaaaagag cagatcgaag
    20761 acttgacctt tacactgcaa gaaatgaaag gaacttcatt atgacaaatg attggctgat
    20821 ccaacaatta gatgaattaa gcgaaaaatc agaatatcaa gaccgtgcgc tcttatatga
    20881 aataaagaaa ctgatggctg agatagacga acgaacaatg cagctgcaag gagaaatcga
    20941 cggacaatct tggaatcatc tcaactggtg aaattttctt ataaataaca aagataaaaa
    21001 tgtgcacagc gacaattatt tgctacaata agaatatgaa gcgtttgata acgctattat
    21061 acataaaaat tggtggtgaa aaaaatgtat gatgaaaatg agtggggaga aaatgttcgg
    21121 atgacccgaa gaagacagca aaaacaggca gctcgtaagc ggatgatcat gattggggca
    21181 gcagttgccg tgtttttgat catagcagga ggagttgcgg gtctatattt tcatggaaaa
    21241 aattcgtcag aagcgaaaag tacgaccata acggaaacaa cgacaaagaa aacagagacg
    21301 aaacaaacaa gtgagtcatc aactaaaaca aaagagacac aaacttctga agaaaaaaca
    21361 gcaaatggtt tgccagagca acttcaagca atcgtaaatg attattcgaa aaagatcgaa
    21421 gaaaagacgc ctgcattgat tgaagaatac cagacagaaa ttcagggaaa tcaagaggga
    21481 atagctggtt tatcagcagt tgctaaccaa aaagcacgtg agcttcaagc aatctccgac
    21541 gaagggatca gaaaattacg tgcagcgtat caggcagcag aaaataaaga cggtgtagac
    21601 ttagatactt tgatcaatca attatcggct aattatacga atcatgtcgc taagatttcc
    21661 gatatttatt tgcagactag cgcctctctt caagcagagt caacaagcac acaagacaca
    21721 acatcttctg attctgagac agcagagtcg gtagaagctt ctgaaatgca agctcgaaca
    21781 acacaagaca caacagattc ctctgaaact aataccaaca gcgcgacttc acaggctgcg
    21841 acaaccgtag ttttggaagg agaaggtcca aatcaaatag ccgaacgaac tggcgtacca
    21901 gtagaaacga tcttgtcgct gaatgggatg agcatggaga attatttcct gactcccggt
    21961 gagacgctaa aattgaattg ataaaagcaa taaaaaagaa cgaagcttcc actaaggtga
    22021 agcttcgttc tttttttatt ttccggttct tttttcgcgt acaaagtaat ggatcgtcat
    22081 gtaaagcaag aaaacgagtc caataatcaa tgagattctc gtttcaggat tgataaacat
    22141 gaaaatcaat acgatagcta aaaaagcaag tgtcacataa ttcgtataag gagatagtgg
    22201 cattttaaaa ggatgatcat gcattttatc tgcgtgcttc tttttgaagc gaatctcgct
    22261 gatcaaaatc actacccaag gaaccatacc aggcaaaata cttgagctgt atacgaaaac
    22321 gaaaatattt tctgactggt tgatgaacat tggtagaaga taatttaaga tcagaccaat
    22381 taagattcca cctgagattg ctacgacagg ataaaaaggc gcaccgtgac gagatagttt
    22441 cagaaatttt ttagacatct ttccatttat ggcaagtgta tagaccatac gacttgcgct
    22501 gaaaatccct gaattgcaac ctgataatgc cgctgtaatc acgacaaaat taatcagtcc
    22561 tgcagcaaat gtaatgccga ctttagaaaa agtttgtaca aaaggtgaac caatctcact
    22621 caattgattc cacggataga tactcacaat gataaaaata gcaccaatat aaaaaatcaa
    22681 gattcgaccg atagtggact ggacagcttc aatgatcgtc tttttaggat tctctgcttc
    22741 accagcagtc attccgatca gttcgattcc ttgataggaa gcaacaacga tcgataatgc
    22801 aaagaaaaat cctttgacgc ctccagtgaa gaatccacca tttttccaaa gatttgaaat
    22861 ccctactgcc tgaccatgat taccaaatcc aaataagatt accccaagac ctgcaatgat
    22921 catcaaaatg atcgttatga ctttgatgat cgaaaaccaa aattctaatt cgccaaacgc
    22981 cttgacagaa ataagatttg ccagcataag aaacaagacg acaatgattc cgggaataat
    23041 tgccggcaaa tcaggccacc aataattcat ataagatcct acagcaatca cttcacttat
    23101 tcctacgaca atatattgaa aaatattact ccaagcggtc aagtagccag ccacaggatg
    23161 gatgtactcg ctcgcgtaat ttgcaaaaga accagtcgaa ggatcaacgt aaagcatttc
    23221 gcctaaagca cgcataatca aatacaaaaa gaatcctgca atcgcatagg ctcccatcac
    23281 tgagggccct gtccaacgta tggttgagga ggcacccata aacagaccga caccgatcgt
    23341 tccacctagt gcgatcattt ggatatgacg ggcactaaga cttcgagtta actttttttc
    23401 ttccatacaa aaactccttt cgttgttgag tcaacaagaa aaaataatca aacgtttcaa
    23461 cacatgtttt tcattattag gttagcctta ttgaactctt gatgtgattc tacagaaaat
    23521 tttcataaaa tgcgaggaaa attcgaaaaa aactttttca tcattctact tgaaaatgct
    23581 ttccatttta gttaaactat atttaaataa aaagttaggt aaacctaaaa aggagtgtaa
    23641 gatttggaac gtacggccat tgtaattcaa gacctttcag ccgcttatca agggaaaact
    23701 gttttgaaaa ctatcaactt gacgataaat ccacaaaaaa ttaccggtat tatcggacca
    23761 aacggtgcag gaaaatcaac atttatgaaa tctttgctag agttgatacc agctactact
    23821 ggtaaagtca ccttttctgg acagccggta aactcagtcc gtaaaaaaat agcctacgtg
    23881 gaacagcgaa gtgaattgga tctttccttt ccaattgatg tattaggcgt tgtactttta
    23941 ggaacatatc catctttacg aattggacaa agacctggga aaaaagaaaa agaacgtgca
    24001 agacaagctt tgaaaaaagt agggatggaa gaatatgcca aaagacagat cagcgaacta
    24061 tcgggtggac agcttcagag agtttttatt gcaagagctc tagcccaagg agcagaatgg
    24121 atctttttag atgaaccatt cgtagggatt gatgcgttaa gtgaacgaaa gatctttgac
    24181 atcttgcagg aattgaagaa ttcaggaaaa acgattttga tcgtccatca tgatcttcat
    24241 aaagtagaag aatatttcga tgaggttatt cttttaaata aacagctgat cgcttccggt
    24301 ccagtacaag aagtctttac atcgaagaac cttcaattgg cctatggtga gatcatcaga
    24361 catttagtaa aaggaggaga gaaaaaatga tccaatcctt tattgatgga ttaatgaatt
    24421 atcaattcct gcaatacgct ttggtcactt ccatgcttgt aggacttgct tctggagtca
    24481 tcggttcttt tattatttta cgagggatgt ctttgatggg agacgctatc tctcacgcag
    24541 ttcttccagg tgtagccatt tcttatatgt ttggttttag ttacatcttt ggagccactg
    24601 cttttgggat gctaacagca gcagtgattg gtttcgtgac tcagcggagc cgattgaaaa
    24661 atgatacagc gattggaatt gtctttagct ccttttttgc attagggatt atcttgattt
    24721 catatgccca gagtgcgaca gatttgtatc atattttatt tgggaatgtc ttagctgtta
    24781 gggaaagcga tttgttgctg acagcactcg ttagtggaat cgtcttgata tttgtctttt
    24841 tcttttataa agaattgaag atcacatcat tcgatccgac aatggcaaaa gcttatgggc
    24901 tgaacacatc gctgatccat tatcttttga tgttcttttt gacattagtg gctgtagtca
    24961 gtttacagac agtaggaaca atcttggtga ttgccatgtt gatcacacca gccgccacgg
    25021 cttacttgct aacgaaccat ttactgaaaa tgatcattac agctgcagga atcggtatgc
    25081 taagtgcagt tgtcggtgtg tttttcagtt atagttacaa ttggccatca ggagctacga
    25141 tcgtgttagc atgtaccgca ttttttatcc ttgctttttt attttctcca acaaaaggaa
    25201 ttttatttca gaaaggaagc agatagatga aaacaaaaaa atcactcttt ttaatcttgg
    25261 cagttagttt cttggtatta gccggttgcg gaaagcaggc ttctgaccaa gcggatgaag
    25321 gaagtaaaga aaagttgtca gtcgtggcta ccaattcgat cttggcggac atggcaaaag
    25381 aggtcggaac agatcaaata gatatccaca gtatcgtacc agtcggaaca gatccgcatg
    25441 aatatgaagt actaccagaa gacatcaaaa aggcaagtga tgcagatgtt attttataca
    25501 acggtttgaa tcttgaaaca ggtaacagct ggttcgataa cttgatggaa acggctaaaa
    25561 aagaagaagg aaaagattat tttgcagtta gcaaaaatgt agaacctctt tatttaacta
    25621 gcggtgaaga acatacaaaa gcagatcccc atgcttggct agacctatct aacggaataa
    25681 aatatgtgga ggaaatcgca cgtatattct ctgaaaaaga tgcagaaaat gcgacactct
    25741 ataaaaaaaa tgcagaagca tatgtggaaa aactaaaaga attagataca caagcgaagg
    25801 aaagttttgc ttctatcgaa gagaacaaaa aattattagt aacaagtgaa ggggcattca
    25861 agtatttttc acgagcatat gatttgccag cagcttatat atgggagatc aatacagaaa
    25921 gtcaaggtac gcctgatcaa atgaaagcga tcattgatca gataagagca aaagaagtac
    25981 cggttttatt cgtggaaacc agtgtagact caagaagcat ggaacgggta gcaaaagaaa
    26041 cgggtttgaa aatctatgat aaactgttca ctgattccat agcaaaagaa ggagaacaag
    26101 gagattctta ctatcagatg atgaaatgga atattgaaac gatccatgaa ggtttgtcac
    26161 aaacaaaaga aagctgaaaa gtgcaaggtc tagcttgagg agcagacctt gcatttttta
    26221 taagagatat gaaaaaaaat tatgctgttt ttcgtcgtac agataccttt tttgaagaaa
    26281 ttccgttata gttagagtaa ggaaggaagg tgtggctatg aattttacac gtaaagataa
    26341 atatacgatt aaagaagaag tctttcgatt gatcagccag caggaaaata ccgacaagga
    26401 tcaagacctt caatttcttg aagcagaatt agctaaatta ttcatagcaa atcaaaatga
    26461 gacaacagaa ccagatgaag agctgtacat aggcaaatgg ttgtttgatg aatttcagcc
    26521 tttagatgaa aataccggta tcggttattt tgtgttcctt tctccagtcg gcttcacacc
    26581 tggcgtatta gttaaacaaa acgacgaaat aaccgtcaaa gcatggcctg cattattttc
    26641 agtcatcaaa gcagaaggag taatcaaaaa gatccagtcg gcctattata aaagtcaatt
    26701 tattgccaac agcaaattaa tgaatatgtt ttataatcgt ctagtagaaa ttgctaggcc
    26761 tgggaaaatc ccgcaaacat tcacgtacac acagcaaaaa aatctctatg aagaagtatt
    26821 gtattcccgc gatctgtctg agttttcgcc atgggaagag gtaaggctcc atgaactgtt
    26881 aagtacaaaa ccagtttcat tgaatttttt taatgcatta gataacaaaa gaaaattaat
    26941 ttcaacaaat tattgggaag tatttgctgc aagaaaaaaa tggatggaag aaaagaagat
    27001 tgccgatcag cagatccagt ttgtccagct gaaaaaagaa aaactatatt atgggttata
    27061 cagccgttaa cagcaagcga ggaagaccat gacattaaaa gaagaaatca tacaagaaag
    27121 caaacgatta ggaattgaca aaatcgggtt caccacggcg gaaccttttg attcattaaa
    27181 agaatcgttg gaagaacaaa aagcagctgg ccatacttct ggctttgaac atccaaatat
    27241 cgatgagcgt ctttatccag aaatgacatt tgatcagccg aaatcgatca tttccattgc
    27301 acttgcgtat cctactagga tccatgaaga agtgcctagg gatgaaaaac gcgggcagtt
    27361 tgctcgtgct tcttggggga tcgattacca tgatatactc agagaccgtt tgaaccgatt
    27421 gatcgaattc atccaaacaa gagcagaaaa atggcaaaaa gaagaggaat ggcgtctagc
    27481 tccgcaagta gatactggag aactgatcga tgtcgctgta gcacagcgag ctggtttagg
    27541 atttattgga cgaaacggat tgttgatcac tgaagagttc ggttcatttg tctatcttgg
    27601 tgagatcgtt actaatattg tatttgagcc tgataaacca ggagaatttg gttgcggtga
    27661 ctgtatgcgc tgcgtgactg cttgtccgac aaaagcattg cttggtgacg gaagaatgaa
    27721 tgcccagcga tgtttgtctt atcaaactca gacaaaaggg atgatgccgg aagaatatcg
    27781 taaaaaaatg cgaaatgtca tttatggatg tgatatctgc cagttagtct gcccttacaa
    27841 tcaaggaaaa gattttcatt ttcatgaaga gatggaacca aaagtagacg aagtatatcc
    27901 aaaattaaag ccaatgcttt cgatgtctaa taaagacttc aaacagcaat ttgggcattt
    27961 agctggttca tggcgtggga agaaaccttt gcagagaaac gcattgattg ctttagctaa
    28021 tctaggtgat cgaacggcat tgcctgagat ttcagaatgt ttaacagatg tccgccctgt
    28081 catcagagga acagcagctt gggctattgg acgtctagga acaaaagaac cggaaaaatg
    28141 gcttgatgta ttagatgaaa tattagctaa agaaacagaa gaagtcgtta taaacgaatt
    28201 ggaacatgcc cagaaattat tgcggagaaa gttaaaataa aaaaatagtt aacattattc
    28261 aaagtttttg tccagtaatt gttgtaacaa ttactggttt ttttgtattt tttacatttt
    28321 tttaagagaa tttgataaaa atcaaaaatt tttttgcttt tttttatgga ttattgcagt
    28381 gaatgttaca gaatctgcgt ttttcattca aaaataaaaa aacatagaaa aacgattact
    28441 ttg
//
LOCUS       DACECZ010000023        27825 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.023, whole genome shotgun sequence.
ACCESSION   DACECZ010000023 DACECZ010000000
VERSION     DACECZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.023"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(338..2230)
                     /locus_tag="IWO35_000975"
     CDS             complement(338..2230)
                     /locus_tag="IWO35_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0316048.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2227..3954)
                     /locus_tag="IWO35_000976"
     CDS             complement(2227..3954)
                     /locus_tag="IWO35_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0316049.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3970..4620)
                     /locus_tag="IWO35_000977"
     CDS             complement(3970..4620)
                     /locus_tag="IWO35_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0316050.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4824..5120
                     /locus_tag="IWO35_000978"
     CDS             4824..5120
                     /locus_tag="IWO35_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0316051.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5312..5932
                     /locus_tag="IWO35_000979"
     CDS             5312..5932
                     /locus_tag="IWO35_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ0316052.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6024..7190)
                     /locus_tag="IWO35_000980"
     CDS             complement(6024..7190)
                     /locus_tag="IWO35_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0316053.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7202..7555)
                     /locus_tag="IWO35_000981"
     CDS             complement(7202..7555)
                     /locus_tag="IWO35_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0316054.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7683..8705
                     /locus_tag="IWO35_000982"
     CDS             7683..8705
                     /locus_tag="IWO35_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ0316055.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(9144..9578)
                     /locus_tag="IWO35_000983"
     CDS             complement(9144..9578)
                     /locus_tag="IWO35_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316056.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9688..11547)
                     /locus_tag="IWO35_000984"
     CDS             complement(9688..11547)
                     /locus_tag="IWO35_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0316057.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDHSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11665..12825)
                     /locus_tag="IWO35_000985"
     CDS             complement(11665..12825)
                     /locus_tag="IWO35_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ0316058.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13132..14556)
                     /gene="araA"
                     /locus_tag="IWO35_000986"
     CDS             complement(13132..14556)
                     /gene="araA"
                     /locus_tag="IWO35_000986"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ0316059.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14572..15270)
                     /locus_tag="IWO35_000987"
     CDS             complement(14572..15270)
                     /locus_tag="IWO35_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0316060.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15227..16888)
                     /locus_tag="IWO35_000988"
     CDS             complement(15227..16888)
                     /locus_tag="IWO35_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ0316061.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17106..18506
                     /locus_tag="IWO35_000989"
     CDS             17106..18506
                     /locus_tag="IWO35_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ0316062.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18758..19837)
                     /locus_tag="IWO35_000990"
     CDS             complement(18758..19837)
                     /locus_tag="IWO35_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0316063.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19923..20882)
                     /locus_tag="IWO35_000991"
     CDS             complement(19923..20882)
                     /locus_tag="IWO35_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ0316064.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20894..22417)
                     /locus_tag="IWO35_000992"
     CDS             complement(20894..22417)
                     /locus_tag="IWO35_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0316065.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22427..22990)
                     /locus_tag="IWO35_000993"
     CDS             complement(22427..22990)
                     /locus_tag="IWO35_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0316066.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22996..23859)
                     /locus_tag="IWO35_000994"
     CDS             complement(22996..23859)
                     /locus_tag="IWO35_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0316067.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23873..24769)
                     /locus_tag="IWO35_000995"
     CDS             complement(23873..24769)
                     /locus_tag="IWO35_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0316068.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24944..26215)
                     /locus_tag="IWO35_000996"
     CDS             complement(24944..26215)
                     /locus_tag="IWO35_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0316069.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26205..27722)
                     /locus_tag="IWO35_000997"
     CDS             complement(26205..27722)
                     /locus_tag="IWO35_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0316070.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT         8124 a         5864 c         4819 g         9018 t
ORIGIN      
        1 aaactggctt ttgaacaccg tttaccaaga ggttgtgagc ctgccgttta actccgagtc
       61 acaaggagca atttcaaaaa acagcttctc aggtttgttg actattgtga cttgtgaccg
      121 aggagttggc aggagaacac cgtttaagga agaggttgta aaatcagcgg tttgacctga
      181 gtattagaga tacaaaacgg aaaaatagct tctcatattt tgccgttttt tgagctaata
      241 ccgaaggaca gctgattgaa caccgtttat tcggaaaaag ggactgtgac acgacttttg
      301 tcacagcccc taaaaaaaat aataaatttg tactattcta tgaagcttct tcttcagaaa
      361 actgactgtt ataaagatct gcatagaatc cgtctttttc catcaatgta tcgtggtttc
      421 ctgtttctac gatcgaacca tgattcatca caataatgtt gtctgcatcg cgtattgtag
      481 acaagcggtg agcaacaacg aaacttgtcc ggttttccaa taggcggttc atcgcttttt
      541 gaatcagtat ttctgttcgt gtatctacac tagatgttgc ttcatcaagg atcaggacat
      601 ctggatttgc taagaatgcc cgcgcaattg tgattagctg acgctgccct tgcgaaatat
      661 tgcttgcatc ctcgtttaat acagtgttat atccttctgg caatttacga acaaaatcgt
      721 ctacgtgagc agctttagca gcctcaatga tttgttcgtc tgttgcatcg tcgttcccat
      781 atttgatatt gtcatagata cttcctgtaa acagccaagt atcttgcagt accattgaaa
      841 actgtgcgcg caattcatca cgtgaaagat cacgggtatc tacaccatcg taacgaatac
      901 tacctccaga aacatcgtag aaccgttcaa gaaggttgat cagtgtcgtc ttccctgcac
      961 cagtcggacc tacaatagcg accatctcac caggtttgac atccaagtta aaatcagtca
     1021 ttagcaagtt atctggtgtg tagccgaatt gaacattttc aaaacgaact ttgtatggtg
     1081 tatctttttc ttctggaagg ccagattttt cattggacat ctcttcttca tccaacactt
     1141 caaatacccg ttcggctgaa gcaactgttg attggatagt gttcatcaaa cttgcaagtt
     1201 gagtgattgg ttgagagaac tgtgttgtat attgcaagaa cgcttgaaca tcccctaatg
     1261 tcatgttccc atttgcaact tttactccgc ctagtacagc gacgaacaca tagcctagat
     1321 ttttaacgaa attcatcaac ggcatgatga ttgccgaaat aaattgagct ttccaaccag
     1381 cggcatacag attctgattt tctttttcaa atacttcctg gtctttttca gtatgattga
     1441 atgttttaac aatggtatga ccaccatatg tttcttctac ttggttattc aataaaccta
     1501 aacttttttg ctgatctgcg aaaaacactt gtgatttcgg cgctacgatc atcacgatga
     1561 tcaagcttaa agggatcatc aataacgcga ctaacgtcaa ttgccagctg attgaaagca
     1621 tcatcacgat gaccccaatc aatgtgactg cactagttac aaattgagtc aaattttgct
     1681 gcagcgtgct tgcgatgtta tccatatcat tgattgctcg agacatgata tccccatttg
     1741 tatgtgtatc ataataggaa atcggtacac gattcatttt ggcttccaaa tcatgacgta
     1801 attcataaac tgttcgttgg gaaacacgtg tcataataaa ttgctgtaaa aagttgaata
     1861 cagcagaaat caaatacatc acgattactg tcgcaatgat ctgcgcgatt ttatcaaagt
     1921 ctatcggaaa ttttgttact tgttggcctt gttgcatctg ttgagcgcca gccattacac
     1981 ctttgaatat ttctgtggtt gctttcccca agacctttgg tgtttggatt tggaagatca
     2041 cggctgctat tgctagtaca aatacagcga tgatcgcaac catacgttta gacatatagc
     2101 caaacaatcg gcgtaccgtt ccccagaaat ttttaggctt ttcacctttt acaccgagat
     2161 ttctgccagg tccaccatgt cctggaccgc ctgctggtcc atgagaggga cgtgtgtttg
     2221 ttttactcat gctaagtcct cctctcttag ttgtgaatga acgatttctt gataagtttc
     2281 gtttgttgct aatagttctt catgtgttcc tttgccaacc agttttcctt catctagcac
     2341 aaggatcata tctgcatcca ttactgtact tactcgttga gcaactaaaa cggtgatact
     2401 ttctttcata ttcgttttca atgcttggcg aagattggca tccgttttga aatccaacgc
     2461 tgagaaggaa tcatcaaaca catagatatc tgctgttttg actaatgccc gtgcaattgc
     2521 cagacgctgt ctttgtccac cagagaagtt tcctcctcct tgttccacat ggctatctaa
     2581 gccatcttcc atctctgaaa caaaatcttt agcttgtgcg atttctaatg ctttccaaat
     2641 catttcatct gttgcatccg gcttaccata ctgcatattg tctcggatcg ttccagagaa
     2701 cagaactgct ttttgaggaa caaagcccat taattctcgc aaattatatt gagtcatgtc
     2761 actaatgtcc gttccattta tttggatcga accagattcg atgtcatata atcgagggat
     2821 caaattgact aaggtcgttt tacctgaacc agtcccccct atgattgcga cgatctcgcc
     2881 cgacttggct tggaaatcaa tatcttccaa tgcaagtttt tctgctccat gatatcgata
     2941 attgacatga ttgaaggcca atgttgcgtt ttttccagca aaagaaactg ttttaggatt
     3001 ttcagggtca tggataccga ttttttcatc caacacttca ttaatacggt cagctgaagc
     3061 ttgtgcacga ggcaccatga taaaaatagc ggaaagcatc ataaagctga tcaagatctg
     3121 catcgcataa gtcataaatg caatcagatt accgacttcc aaagtcatat ctgctatgta
     3181 gtgtccacca aaccaagtga ttgctacgtt tgtaccgctc atgatcaatg tcatcattgg
     3241 catcatcaat gcgacgatcg tattaacttt gattgccgta tttgtataat ctttattcgc
     3301 taaatcaaaa cgattttctt caaagcgtgt cttatcgaat gctcgaatca ctctgacccc
     3361 tgtcaatcct tctcgaaata ccaggtttaa acggtcagtt ttcttttgca tacttttgaa
     3421 taatggaaca gcaaagtaca tgattccgcc gatcaaaata atcattactg ggatgacaac
     3481 taagaagatt tttgtcaact gatgatcttt ttgataagca agtatgctcg cgccaatcaa
     3541 cgtgattggt gcattgatca tcatacgtaa aaacatttgt gtaaccattt ggatttgagt
     3601 cacatcattg gtcgttcttg tgatcaaaga ggccgttcca tatttatcaa acgcatcttt
     3661 tgtcagactc tctgattttt tatagacatc cgtacgcaat tgcttaccca gtttctgtga
     3721 ttcacgagta gcaaaaaatg tatttgcgat agcagctaag atactgatca aagaaaagcc
     3781 gatcatcaca aaacctgtat gccaaatata atcgacatct ccttgcgcta cccctttatc
     3841 aataatattg gaggtaagtg tcggcaaata taaatctgcc agtacttgga tcaccatgaa
     3901 aactgcagct gcaattgctg aagtcaagga gatccgtttt acgattttta gcatctagtt
     3961 cccttctttc tatttatata ctccgttttt cagccaagaa agtttcaaat tgaattcact
     4021 gattgcttgc tctactgagt aatcttcaga tgttgaaaat aagcgatacg aatgcgcaat
     4081 tgttgtaaat acagtattca tcactaattg gatcagtaat tccaattcat gatcattttt
     4141 tactttcagc ttcgtccgat cgatcaattc aagcaactct tttgcatctt gtttgtgttc
     4201 tttatgtgga tcgtgaggcg ttttttggcg actcgtatga ggagcgactt ttcttgaggc
     4261 acggtaatcc atgttcataa acaagttacg ataaaaagaa gcattctcgc cttccaaaat
     4321 ctcataaatc attttagaaa aataggattc gaatcctttg aatagatcac catcggattc
     4381 tttcattagt tgtaacatat ctttagagct gtcacgtctc attgtctcaa agtaataaaa
     4441 ataaagatcc tctttatctt caaaatactg atagaaactg cctctaggga tatccgctaa
     4501 tttgacgata ttagcaatcg aagcttcttt taagggtact ctagaaaact cgattctcgc
     4561 agcctcgatt agtctttttt gtttttcttt aggcaaatgg aaaaatgtct gtgtcggcat
     4621 atggattctc ctttctttca agatgacaca ttgtcatatg acgctatgtc attataacac
     4681 ctcctacctg ttttgcaaga catttgcata gtttttttca ttatcatccg atacattaaa
     4741 aaagtgataa gaggaatcct tccatttgaa ttaatgagag ttgcccatta aaatagccac
     4801 atactaaaca aaggatgaga taaatggccc aatcaattag tgtccaagac ttcaataaat
     4861 tgcctctcga tgctgacagc atcgttttag atacaagaaa taccttagat tacacaaaaa
     4921 accacttaag ccactcgctt tctatccccg ctgctatctt gccaagacaa ttaaaaaatc
     4981 tagatccaaa gaaactttat tatattctaa gctatacagg caggcgatca gaagcattag
     5041 ccgatcaact tactgctaat ggttttcgtg ctgtttatat cattggtggg atggaagctc
     5101 ttcagtcgag tgttgcttga atattgcttg ttagattcaa aagaggctgt gacaaaaaat
     5161 cgtgtcacag cctcttttat accctgtctt tttttagtta tttattcttc aaccttatca
     5221 atatgacttg atagcctttt ctttttcgag taaactgatt ttgttagtca tagttataac
     5281 aatcatagca ataaaaggag aaggtaagca tatggcatct gttttagtag tcaaaggtca
     5341 tcccctaact gcagaagaat cgcgcacagt taaggcattg acgtcttttt taacaagtta
     5401 taaagaaaat catccagatg atgaagtgac tgttttggaa ctttatcgcg atgatattcc
     5461 ggaaatcgat gaagaattat tatcaggttg ggaagcatta cgtgctggtg cagaattcac
     5521 ttccttatca gaaagtcaac aacaaaaaat tgctcgtttc aatgaattga ctgatcagtt
     5581 ccttgctgct gataaaatcg ttatcgcaaa cgccttatgg aatctaaata tcccaacaaa
     5641 attaaaagct tggttcgata ccgtcaatgt agctggaaaa acgttccgct atacagaaaa
     5701 cgggccagaa ggattagtga caggtaaaaa agcactgcac atccaatcta atggcggtgt
     5761 atacaacgga caagactttg cctctcaata tgtaaaaggt attttaaact ttgtaggcat
     5821 cgatcaagta gatcagctat tcatcgaagg aatcgattat gaccctgacc gtgcagatga
     5881 gttgatgcaa aatgcattag ataaagctgc tgctttaggt aaatcatttt aaaaattgag
     5941 ataaatgcat acaaaagaga cagaggaaaa aatcttcaaa gcagcatttg tgccttggat
     6001 attttcccat ctgtctcttt ttgttattct ttctgctttt tcaacggatt ttcttcataa
     6061 agagaagaga ttgttgtgta ccattcaaaa acatgagtaa tcactacttt agctgctgca
     6121 taaacaggaa tccccaagat cacaccgatg attccgaaca atttcccaga agtcagcaga
     6181 actaataaaa tcgttactgg atggattgct aactgattcc ctagtactaa tggagagatc
     6241 aagcgtcctt ctattgtctg ctctactgca aaaacgatca gtactttgat gatcatgaat
     6301 ggacctgcta caatggctaa aaagatagcc ggaatcatcg ctaaaaatga tcctaagtaa
     6361 gggatcaggt ttaaaaaacc cgcaataatc cccaacgtga cagcatactc taaaccaatg
     6421 accgcaaaac caatcataaa catgattgcc acagcaaaag ccacagttag ttgtcctctt
     6481 atataagaag aaacttgact attcatttca ctcagcacat tcaacgtcgg tttttgcatg
     6541 cgtgtcggta aaaatttgac aaaataaggt gctaattgtt taccgtcttt caatagataa
     6601 aataagataa acggcatcgt cagcagagcc actaaaatag tcgctaccgc gccgacaaaa
     6661 cttccaagac tctgaactgt actcttagat atatcttgga tcatatcgcc tgccgaattc
     6721 atgaacttat cacctgctgt ttccaactgt tcgcgaaatt ggttgaacag cgggtcccgc
     6781 aaaatctcgg ttaatttatt atcaatcgta tctacatatt gagggaagtt actgatgaag
     6841 ctcactgttt gttcctgaat cttagggatg atcactacgc tcccccatgc gattagtgca
     6901 acaaccaaaa caaacagccc aatgatactg taaagccttg gtacttttcg cttttcaaag
     6961 taatcaacta ccggattcat caagtaatac aagattcctg ctaaaatgat aggcagacct
     7021 acgattgcta aaaattgcca gattggtgca aacaaatatg aaacttttgt aaataaaaag
     7081 acgatcagca atattaacaa tacaacaagt aaggctgtga ctacttgatt atttaaaaac
     7141 cagcgccaaa accacgatac cggtctgttc ttcttatttt ttccttccat aattcctcct
     7201 gttagttata aaaaatttgg cttcccaaac caatagcagg cagtctatgc ggatgaagat
     7261 agattccatt tgcaatttgt ccttcttctg gacaagcatc aaaaatcaat gatacgtgag
     7321 ctactgtctt taaattccca ttggccatcg gaccgacata atcgatcgta tatacctgat
     7381 catcaaaagc aattgttccg cctgctttca aagaaaaagg ttcgtctttt gatacttctt
     7441 gaatcaccga atattttcgt aaggcagctg ttgctgtttt atcgaacaaa atcagcatgg
     7501 gttcttcctt gtttattgct tctgtcccaa tttctgtaat ctttgcctga atcattgcac
     7561 tctctccttt aattgctttc aaaaacagta taacacattc tcattcttta gccgtgtgga
     7621 ataattatgt gatagaataa aaacgaaaac tatttcatta caattaggag gaatcagctc
     7681 atatgatcga aaagataatt ttaggaacct atacgcgtcg cgaaagtaaa ggaatctata
     7741 cgatcgacct tgatacagaa aaagaagaat tagccaacct ttcattagct gttgaagaaa
     7801 atagccctac ctatgtagca aaaagtaaag ctggcaatct ttacacagtt actagtgtgg
     7861 atggattagg tggtgccggt gcgtacgata aggattttca ttttttgaat gcggtaacag
     7921 agtctggcgc tcctttgtgt tatgtagcgg ttgatgaagc acgtcaatta ctatacgggg
     7981 cgaactatca taaaggtgaa gtaaatgtct accatatatt aggagatggc gaactgaaag
     8041 cagcagattc aatattccat caagaagcga caggtcctca taaaaaccaa gatcatgcgc
     8101 atgtccacta tacggatctg actcctgacc aacgattagt cgtatgtgat cttggtacag
     8161 atcgtgtgta tacttatgat gtgtccgaaa acggcaaatt gaatcttgta actaccttca
     8221 ctgcagagcc aggaacaggc cctcgccatc ttgtctttca tccaaatgga caattcgctt
     8281 atttatttgg tgaattagac agcacagtcc gcgtgctttc ttataataaa gaaacaggtg
     8341 cttttgaaga attacagaaa atatcgacct tgccagaaga attcgacggt gaaaatggag
     8401 gggcagctat ccgcatttca aatgatggca agttccttta tgcttctaac cgaggacata
     8461 actcgattgc tgtattcgca gtttttgaag atggttccca cattcaaaac aagcaaatca
     8521 tctcaacaga aggggatttt ccccgcgatt tcgcactaga tccaagcaat aattttgtcg
     8581 tgtgtgccaa tcagaacacc gataatctga cattatatcg ccgtcatcct gaaacaggat
     8641 tactggaatt actgaaaaaa gatgtctatg taccagaatg tgtctgtgtt tattttgatc
     8701 gttagtaaga aaaggagaat gaaatgaatc agcaagacgt attagacaga ctacgggaag
     8761 aactaaatat tcctttcttt gatgggaaaa tcgaagataa agagtatagc gaagaggaat
     8821 atcagaaact caaagccgat ttacagaact actttgaaca atatgtacag aatgtagaaa
     8881 actaatcagc tttccatact atctgaaaag aacatcagga caagactttt ccgtctatcc
     8941 tgatgttctt ttctacaaat aaaaaaccgc cgagatggtt agactcgacg gaaaaggagt
     9001 atttactcga ttttgagtaa gtacaaggtt gttgttggtt ataggtatgt cattatcata
     9061 aaggattttg atcactctat cataaaggta atattacatt tatattagat aactctagtt
     9121 tttattgtta tcaaagattt cttttatttt ttcaacaaca aattagtcat tttttggatg
     9181 gcagctgtca attggtctgt cgcttggtct tgttttgctt cagatgtatt gaatagatct
     9241 tcatctaccc ctgattcaag atcgatactt tggatcattt cttcacagcc tgctacatat
     9301 gaaagataag ctttttcaaa atttttatga atgcccattg cttttgctgg tggacgcaaa
     9361 gtactgactt tcataagcat tgcccgatat ttttttgttc cttcttcaaa taacaggatg
     9421 atctcttcaa gagtcgattt gtccagttca cttgtttttc cttcatcaat tgcgtttctg
     9481 atcacttcat atttcggatg cattccttct ccgattgctt ctgtttcctt gacgatttga
     9541 tcgatggact gcacataata gccaatattc ggtaacatgt tcgttttcct actttctcca
     9601 ttgttgttat tatttattct acttgttttt atcctcaaat gacaagtact tgaaaaaatt
     9661 taatggtttc tatacagctc gccattctta ttcgtgctca aagaaaccat ctgctaaact
     9721 atattgtttc cctgcgacaa tgtcatattg gatcaattga tattcgtcgt aaatctcttg
     9781 ttgcgagcga ttgaattgcg cttctttata ccactgtccg ttttgatagt acagttctcg
     9841 ttcaaacgcc ggaagttcct gttctaatga taaaagcagc tgataaaagc catttgttgt
     9901 ctgattcgtc tgttccatta gattcgctgc aaaataaaac ggactggtga ttgcatcgtg
     9961 agtcgtttgc ttttctaatt ctcctttact gtcgaacata agaaattgtg tttcatgcaa
    10021 tgtttgtttg tcattttttg cttggatcgt atctgaatag atccctggaa gatgatcccc
    10081 ccaaaaaaca actaaagtcc ttctagaaag attcttcaat tcttccgtga actgcttcag
    10141 tgccgtgctg ctataggaaa tatcttgcag ataattctct aagtcaagtg tcccacttcc
    10201 atctgaagtt tttgcaggat agtctagatt atcatacttt ccatcgtagg gcatatgtgt
    10261 ctgcatcgtg actaaatgaa tgaactgagg tgttttttct tccttgagaa gtgtcattac
    10321 ttctttgtaa gctgactcat cagaaatata tggattattc tctatcgtat ccgtataggt
    10381 catcgtacgc tcgctgatga actggtcaaa tcctaatgtc tgataaacat cctctctttt
    10441 atacatcgaa gtattgtatg gatggatcgc tgtcgtctgg tatgattgtg cgtttaacgc
    10501 tgaaacgata gaaggaagct gatccatttt tggtacaagc atcgtgtaag gggtggtgag
    10561 ttgcgcatta aataacgcca tagaaaaacc agtcaatgct tcaaattcga tatttgctgt
    10621 tccaccgcca tagttttgag aaagcatatt gccactatat gtttgatcag ctacttcata
    10681 ataatctgcc aatggctcac cagatacttc tacgccgttt aatcgactat gatctgagaa
    10741 actttcactc atcacgaaca cgatgttcgg cgactcttcc tctacagttt tatttttttc
    10801 atcggctagc ttctggtatt tttcagtgat ttctttgatc ttttctttcg aatatccttc
    10861 aggttcatcc attggttcta ccttcagatt gtacaaaaac ccaccgataa aaccagtgtt
    10921 atagtagttc attttctggc tgtaaggtat ccatagtgcc gttttattat atgcttttcg
    10981 caacaaatta tcgggattgt tgaaattact gatataccct agcagtccaa ttgccacaag
    11041 cagtgtcgtt aatcggatca attggaatcg ccttcctttt ttcaggcttc gaaacatgta
    11101 ccagcaaaac aatcctatca cggttcctgc ggcagctaag atcactgtaa acagcatagt
    11161 gccggtcata tctttcagca atccgatttc agtgatcatc ttcaaatcgt ctggataaat
    11221 cggttcttgc cgataactca ttttcatata atttgcaaaa cctaatacac cgatggctac
    11281 gacatacgta agggaaccta aagggacaga tcctgctagt gcaatcagaa aaataagaat
    11341 aatcagcaag acgagacagg ctaagaagaa tttttccgta tgccaagaaa aagcaaattt
    11401 caacgcaagg tctacggata gatcattttg acaccactgc aaaaataaat tactaaaaac
    11461 aacaagaaaa aagatactga tgattgctgc tgttcctttt aaaatctttt tctgtttggt
    11521 cgaaagtctg cttatatatc ttttcaaatg cttgctcctt catttctatc aataaaagaa
    11581 tgttgcttct cttattttag ttgtacgaaa ttcgaaagta aagaacggaa agaagaatta
    11641 atgaaatcca aatattctct ttctttatgg cctttctggt ttctttaata agcggacctt
    11701 tcgtactcca gaaatttttt ctagctgttg tttcacttga ttgagctgtt caaaatcagt
    11761 actgtctatg tcaatcaata tgtagacatg ctcattctta tggttacgcg atatatctgc
    11821 gatcgctagc tcgttctcat tcagcagaga aaatatctct gctaatatat tggttttctt
    11881 ttgataaaat atagtgaatc gaaacggcgc ttggaaaaac attcgtgcgg atggataatt
    11941 gaccgcttct ctaaccgtac cgaacaaaag ataatctttt aaggcttcaa ctgccaaacg
    12001 tccgctgtct gccaaagctt cctgagtcga tccccctaga tgaggcaaca taagtatacg
    12061 gtcattttct aagaactctt cttctggaaa atcagtcaag tatttagcca gatgttgttt
    12121 ttccaatgct tgcataacag catatttatc aacaatttca cttcttccta cattgataag
    12181 tacagcatct tttttcattt tttctatatt cgcctgatcg atcaatcctt ttgtatcttc
    12241 tgtcaacggc agcatgacta cgatgtaatc cgaggtagaa agaactgttt ctaaatctgc
    12301 ttgccgaaca taatccaact gggcatcgcg gatagaataa cccaagacat ccatccctaa
    12361 gctatagcaa cttagcgcta cttttgtgcc tatcgctcca agaccaagaa gaccgatggt
    12421 tttgccttgc aattctcgtc ccacataagc acttcttttg ttttctgcct gttctaaaat
    12481 attaggacct gttagtgttt gtaccatacg acttgcttcg attatcggac gggaagataa
    12541 gagcaaacag cacaaaacaa gctcttttac tgcattagca tttacacctg gtgtattcat
    12601 gacgatcgtc ccgttttcag aagctttttc aacgttgatc gtgttcaccc caacaccagc
    12661 tcttgaaatt gctagcaaat ctggtgtaag ccattcatct ttcactttcg tactacgaat
    12721 aactattcct tcggctggca aatcatctat tattttgaaa tattcccctt gtaacttttc
    12781 tatctcttgt tctggaatag catcaatcaa atgaacgtga tacataaaac cccctcctta
    12841 ctaacaagta tctcgaaatt tctgcaatta gacaactgtt taccatttga cttcctagaa
    12901 gcaaaaagag cagttagagc agagtaggct gagtgataaa aataaggcta tgacaagtta
    12961 ctgccataag ccttattttc gagagcaaaa tattgagggt gagatatctt gataaatggt
    13021 gttccgttag ctgacattga taataagact aaatttcaaa aaagttgaaa agcaaatttc
    13081 gtcaatctct tcttatttct taaaactaag tggctttttc acaacctctt tttatctaat
    13141 aaccactgtt tccaactcaa ctaattttgc ccacgtttca atttgatctg ttgttagatt
    13201 tagcgagaca acggtatgat gaccgccacc tgcctgaatc catgaatgga caccttcatg
    13261 gaaattcggt ttagttttcc ataacacacg tgcaacaggt aaattaggtg cttcagtcgt
    13321 tggttcaaag gcttctactt cattgatcag cagtttataa tgcgtaccga aatctgccat
    13381 cgatacgact accccttctc ctgctttccc atcaaataca agacgcgccg gatcttctct
    13441 atctcccatt gataaaggag aaacaacgat tcttggtttc gtcgcagcaa gcgtcggatc
    13501 tacttccatc atatgggatt ccaagattgc ttcttggcca ctagctagct cgtaagtata
    13561 atcttccatg aatcctgtat ctttccctcg agccatgatt ttcagcaacc gatcaatcgc
    13621 tgcagttttc cagtcgcctt ccccagcaaa accgtatcct tctgccatca aacgttgtac
    13681 agctagtcca ggtagttgtt tcattccata caggtcttca aaattagtcg taaaagcgtt
    13741 atagcctcct tcttctaaga aacggcgaat accgatttcc tgctgagctt gtacttttac
    13801 atgttcctcc catgtctttt cttcataatc accatagtcg aaggtataca attccttgta
    13861 ctcttcaaat aattgatcga cttcttcgtc tgatacgcga tcgattactt gtacaagatc
    13921 tccgatacca aaataatcaa cagtccaacc aaattgaatc tgtgcttcca ctttgtctcc
    13981 ttctgtgaca cccacgtttc tcatgttgtc accaaaacga gccactttta ttccaaaact
    14041 ttcattatag gctactgcaa catccatcca atcagcaatc tctttttgga tctcaggtcg
    14101 tttccagtag ccaacgacga ttttattttg tttatttaat cgagcattga tgaacccata
    14161 ttcgcggtca ccatgagcgg attgatttaa attcatgaaa tccatatcga tcgttttcca
    14221 aggaatgctt tcgttatatt gcgtagctaa atgaagcaat ggtttttgta gaagctttgt
    14281 cccgcggatc cacatttttg ctggtgagaa agtatgcatc caagtgatca cacctgctac
    14341 ttcttctcga taattgacct ctttcatgat ttttgtgatc gtatcagcag taactgctaa
    14401 ttcttgaagg cggattggat aaggcaattg gccattttca ttcagttcat cgaccatttc
    14461 ttgtgcatgt ttctttactt ctcgcagtgc ttcttcccca tataaatgct gtgatcctac
    14521 aacaaaccaa aattcttttt ctccaatatt caacataatt agccactcct tttatttgtt
    14581 ttgtccgtaa tacgcatttt ttccgtgttt tcgtaaataa tgtttatcta aaatccgttg
    14641 aggcagctct tctgaaaatg aattgagctg tctggtaaat aaattcatct tgcatacttc
    14701 atctagtacg actgcgttca ttaccgcact ttgcgcatct ttcccccaag taaatggacc
    14761 gtgtccgtgt aaaagtaccc ctggaattgc taagggatca attttgcgtt ctttgaatgt
    14821 ttctatgata acttttcctg tttcatattc gtacccgctg tcgatttcct gttgtgtcaa
    14881 aaatcgagca caaggtaccg atccataaaa agtatctgca tgcgtcgtcc ccatcgcagg
    14941 aacatccaat cctgcttgtg cccagatcgt cgcccatgtc gagtgagtat ggacgattcc
    15001 tccgatttga ggaaatgaac gatataaaac agcatgagta ggcgtatccg aagagggatt
    15061 gagtttacct tcaatgatat gattgtccaa gtccacaacg accatgtcgt ctgctgtcat
    15121 ctcttcataa ctgactccgc taggtttgat aacgaaatat cgtgtttcag gatcaaaagc
    15181 gctcacattt ccccatgtat atttgacgag tccgtgttta ggcaagtcaa gatttgcttg
    15241 gtatacttct tctttcagtt gttccagcat tttctatcac tcctttttct tgcaattgat
    15301 gaatcgctgt tttttcaatg tcgatcccct cacggtaacg tctcataaat atctgatagc
    15361 ctgctatttc ttctgctgaa ggtgtgatgg tgattccttg gtcttgtcca aacacgtctt
    15421 ctgccaagaa gttttctaaa gacttcccat gtcgatccat catataggct gctaataacg
    15481 cgattcccca cgctccacct tctccggctg tgtccatcac tgtaactggt gcctccatag
    15541 cagatgctag aatccgctgt ccaacttctg gtgttttaaa aatcccccca tgccccacta
    15601 aacgatcaat ttggacattc tcagatttca atatatccat tccgattctc attgcaccaa
    15661 aagcactggc taaatggatc cgcatgaaat tcgctaaatc aaaacggcta cctggtgtac
    15721 gtacaaataa tggccttcct tcatttactc ctgtaatgtt ttcaccggaa aaataaccat
    15781 aagaaagtaa tcccccgcaa tccgtatcgc cttctagcgc tttgttgaat aataccgtga
    15841 ataatttatc tctgcttatc tccataccca aagaattggt aaattcttca aaaatactga
    15901 cccaagcatt gatatccgaa gagcagttat tcgtatgaac catgcctact agttctcctg
    15961 aaggtgtagt gaccatgtcg atttccggat ggacattttc tagttctttt tctaatacga
    16021 tcatagcgaa ggcggaagta ccagccgaaa tgttccctgt ccctttggct acactgttcg
    16081 tagcgaccat accggttcct gcatcccctt ctggcggaca aaccgggatt cctggtctca
    16141 aatttcccgt agggtcgatc agataagccc ctgattcgct taacttgcca gcttcttctc
    16201 ctgctactag aactttaggc aaaatgtgat ccaagtcaat cgctacgtgc gctttttcga
    16261 ttaactgacc aaaaatattt ttcatttctt gatggtaggt tttcgttgaa ggatcgatag
    16321 gaaacatgcc tgatgcatct cctacgccta gtactttctc accagtaagc tgccaatgga
    16381 cataacctgc aagcgttgtc aaaaaattaa tttctttcac atgttcttct ttattcaaaa
    16441 tagattgata caaatgagcg atactccatc tctgcggaat attgtattga aatttttccg
    16501 ttaatattcg ttctgcttct tcagtgattg tatttcgcca agtacgaaat ggtactagta
    16561 actgttcctt gctatcaaat gctagataac cgtgcatcat tgcactgaac ccaatcgacc
    16621 cgattgtcgt aagattctcg ccatagattt cgaatacttc tgctgccatt tttcggtaac
    16681 tgacgcgcag tcctttccaa atatcttcta atgaataggt ccagatacca ttttctagtt
    16741 tgttttccca ctcataacta ccagcggcaa taggagtaaa agatgaatca atcaatacag
    16801 ctttgatcct agttgatcca aactcaatcc ctaaagatgt acgaccatct atgacatcta
    16861 acatactttg cgcatttact tccttcataa tgatttaact ccctccttct cgtcaaacct
    16921 gatcaaaccg tctttttagt agcgcttaca atgattattg tatacttttg tacgtacatg
    16981 tcaagcagtt atttacaaga atacaataaa aaacacttgt tttgtacgta cataagttat
    17041 aaaatgaaat tgcttacaaa atctgatcaa ccgccttctc gaaattctag aaggagagaa
    17101 tacttatgaa aaaagaaaaa aaagtttcct catcgtttat ttattttttt ggcgcatttg
    17161 gcggcattct atttggatat gatattggtg ttatgaccgg cgccttacct tttttacagc
    17221 atgattgggg tttggcaggt aaagcttctt tgataggatg gatcacgtct tcggtcatgt
    17281 taggtgcgat ccttggcggc tctttatctg gtcttctttc tgataagctt ggacgacgaa
    17341 aaatgattct tctttctgca cttattttta tggctggttc tgtcctttct gcatctgccc
    17401 ctcacaatgg ttcttatttt ctgattgcag caaggattct tctaggttta gctgttggcg
    17461 ctgcatctgc cctagttcct gcttatatgt ctgaaatggc tcctgctaga ctaaggggaa
    17521 gattgtcagg gatcaatcaa gtgatgattg cttctggcat gctcctttcc tatgttgcag
    17581 attatttgct aaaagggttg cctgaaacga tggcttggcg cgtgatgtta gggctcgctg
    17641 ctgtccccgc gctgatttta tttttcggtg ttttagctct accagagtca ccgcgttttc
    17701 ttatgcaatc tggccgcttg gaagaggcaa aaagagtatt gaactatatt cggacaccaa
    17761 aagaagctga acaggaattc gaacaaatcc agctgaatgt caaacaagaa aagacaaccg
    17821 gaacttcttg gcatactttg tttttagaaa aatatcgttc cttagtcatt gccggtattg
    17881 gtgtagcagt attccagcaa tttcaaggag ctaatgctat tttttactat attccgctga
    17941 tcgtagaaaa agcgactggg catgcagcga gcgatgcatt gatgtggccg atcatccaag
    18001 gaatcatttt agttgctggt tctttgctct ttttggttat tgcagacaag ttcaatcggc
    18061 gaactttatt gaaaataggc ggtagcgtga tgggactttc tttcatccta ccagctgttt
    18121 taggaactgt actggatgct catacaaatt cgttgttgat tctcttgttt ttatgtattt
    18181 atgtcgcatt ttattcttgc acatgggctc cactgacatg ggtaatcgta ggcgaaatat
    18241 tcccactagc tgtccgcggg cgagcatctg gcctagcctc ttcttttaat tggattggtt
    18301 ctttcctagt tggcttgctt tttcctgtga tgactgcttc tatgtctcaa gaaatcgttt
    18361 tcggcatttt tgggatcatc tgctttttag gggttttatt tattcaagag atcgtaccag
    18421 aaactcgtgg gaaatcttta gaggaaatcg aacaaagtgc atctaaaaaa acttatccta
    18481 aacgtatttc tatccatcat tcttaaagag gttgtgacag aagtggtcag cttcaagaaa
    18541 taagaaggaa ccgcttagtt tccagtcaca aggaacaatt tcaaaaaaca gcttctcatg
    18601 tttgatgaat attgtgactt gtgaccagtt ggtaggagaa caccgtttag tggagaggtg
    18661 gtgaaagaaa cgttcagcct tgagcgatga caaaagctct cacaaacatt gcgacgagta
    18721 accgtaggag caataagagg cggtaaaaac ccgtctctta tttttcgttt tctttcactg
    18781 tttcccgttc aactagtaca ggttcataga gaatatttgg ctgtgcctgt cctttttcaa
    18841 tcgttgtgac gatccaccgt gcggcatctt gtcccaactt ttcttttgga tgcgtcaatg
    18901 ttgtcaattt tttattccct aaatggctta aagaagaatc gtctgttcct acgatcgata
    18961 ggtcatctgg aatatgatag ccattttctg tcaacttatc tagcattaaa ctagctattt
    19021 gatcgttata gcaaatgatg ccatctaaag cttgttcttc tttcaaccgt tgtactgttt
    19081 tttcagcgat tgtttctcgt gtttccgtgg tgaaagtaat gatatcttcc gtcaaaaaac
    19141 gcacattata ctgttcacac gcgctgataa aacctttcat cctgtatttt ccttgaagat
    19201 cgtcgatttt tgtgatgaac aacagacgct gatgtccttt ttttatcaaa tattctgctg
    19261 ccataaaacc gactttcaca tcatctaaac aaatataaga ggcatttaac tcttcataag
    19321 cagcattgat catcaccatc ggaatgcctt gttctctcaa agcaacataa agtgctaagt
    19381 ttgggttata ctgattactt ttcgtaggct caacgatcaa accatctacc ccataagcaa
    19441 tcattttctt caaacactct ttctccaatt cgtgatcgtt attcgtactg gctaaaagca
    19501 atgaataccc agattctcgt aattcatttt cgattcctcg gataatcgac ggaaaaatat
    19561 aatcagagag atatgtggtg atgacaccga tcgtttttgt tctgttcaat ggtgtttttt
    19621 ctttcttata gctgtcatct acatatgtgc ccgacccttt ttctttacgc agatagccct
    19681 catttaccaa cacagcgatt gcttgccgaa tcgtatgtct gctgacgtca tatatttctt
    19741 gcagcttgaa ttctgaggga atggttgtat ttacttcgta ttcctcacga aggatctttt
    19801 gttttatctc ttctgcaata tcttgatatt ttgccatttg ccttgcccct ttttatttgt
    19861 acgtacatat atcataacga gtctgcaagg tttattcaag caaaaatcaa ggtttcttgt
    19921 gctcataagg catacggaac aggttcacca aattctggaa atccttgctc attccacgta
    19981 aagcattgcg cgttcgcgtg ccgattagga ttgtctaacg gatccccttt ttggttcttc
    20041 tctggtcgag catgatagat caaaatatca aagcacccgt cttctgaaac cgtaaacgag
    20101 ttgtgaccag gaccaaattg cttatttttt tcagaagaaa cgaatactgg ctccgataat
    20161 ttatgccatg attcttttaa taaaagattc ttgtgctcat cagcccacag caggcccatg
    20221 gcatagtttt catctgttgc actaccagaa tacgtaatga atattttccc attgcgagta
    20281 aggactgctg gaccttcatt tactgaaaaa ccccgttttt cccattcata ctcaggtata
    20341 gaaaggagga tctgcttccc ctttaacgtc caaggattat ccatttcgga tagataaaga
    20401 ttagaattcc cagtaatttc aggatcttgt tgggcccaca cataatatag ctttccttga
    20461 gaagagaaag ttgttccgtc taaactgaaa gtttcccttt tcgtttttat ttgtcccttt
    20521 tctatccaat ctgtattcat tggatctata tgctgatttt caagtacgaa catgcggtga
    20581 tgatgagatt catcacgtat agttttatga ttgcttgcag caaagtaaat ataccatttt
    20641 ccattgatat aatggacttc aggtgcccag atcaattcac tcatcggtcc ttcttcatga
    20701 gcctgccata caatcaggct tgcagcatgt tctaattcat tcagtgactt tgcccttctt
    20761 aattcaatca cttggtagcc tggaaccgat cctgtaaaat aataataacc atctgtatgt
    20821 ttatataccc aaggatctgc ccgttgccga acgataggat tattataaag tgtcttcatc
    20881 ataacaaatt tcctcacatt ttctttattc taattacatt ccaagagtat ttttctatcc
    20941 ctacatttaa ttcgtcatgt ttttctattg aaaaatagtt tatttcaggt tttatttttt
    21001 ctggttcttc acgtgtattt ttgtccaaca gatcgctact ggttaatgtg tagtgctctc
    21061 cttgatctag caaattgaaa tcatgttcaa tcgttatctt ttgttgttca tttgtgcgat
    21121 tgatcaagaa aatagtcagc tgattgccgt tatctacgat cacactgtcc acataaggaa
    21181 cattttttat cttttcacaa tcataaacag ggcaaatgat ttgttgtttc aatacttgac
    21241 ctttagcata tttcgataag tgttgcatca cataataagt tggattcctc catgcttgtc
    21301 ctttagcatc tgtcagtatt aaaggtattg tgtttactaa caatgattga caactaatct
    21361 ttacgcgatc tgcgtgtcgt aaaatagcca gccctaatga accaaataat aatgtatctt
    21421 ccatagtaaa gtaacaccaa tcgatgggac taccgacttc aaaatcccta tgtctttttt
    21481 ccggttgggc atgtacattc cattcatcaa acgctagata cattgttttc tcacttctca
    21541 aatatccttt tacataatca caagtagcaa cgatttcttc aatttgacga tcgaatcgat
    21601 aagcttttgc aaggtatgtc gctgtatcat cacgctcatt tttaggaggt ttagtcaagt
    21661 cttcttcatc atacttatca atatagttat gcaaagctaa ataatctaca ttctcataag
    21721 cttcttctaa tgcgatacga tcccattcag ggtacgatgg taaacgtggc gtagagcttc
    21781 caactaagat tgtttcaatt gaatcatcca ctaacttcat gacttttgag gcttcatttg
    21841 ctaacctacc atattcataa gcactcttag cccccacttg ccatggaccg tctagctcat
    21901 tccctaaaca ccataattta acgccaaaag gctttttttg cccatttttt attcttaaat
    21961 cactccagaa ggttcctttt ggaaaattac aatattctac taaatttttt gcttcatcaa
    22021 ttccttttgt tcctaaattc accgtgaata tcggtttcgc ttgtacatga ctgatccatt
    22081 taaaaaattc gtggagtcca aaagaatttg gttctatacc tcgccatgct aaatcaattt
    22141 tttttggtcg ttgttctttc ctacctacag tatccaccca atcataacca gatgtaaagt
    22201 ttcctccagg atagcgaatg acacctaatt ctaattcctt tacataagag aggacatctt
    22261 gtctaaaccc gtcaccatca gctgtttcat ggcccggttc ataaatccct gtatagacga
    22321 cacttcccat atgctcaaca aatgagccaa acagccgaga actgatctct cctaccgtaa
    22381 aatcctctct gatttttact ttcacttctt cgtccaacag ctttccctac ctttcatttc
    22441 ctatagtcaa gataatagat acaagtttcc atacgccacc agcaaagata aaaataaaag
    22501 gagggaaaag aaatatgagc agtacaaatc ctaataacaa tacgcaacaa agaatatttc
    22561 tcaaaaaatc aaaaaatact ttccatgcac tttgttcaaa aatttgtaaa aatgtgatat
    22621 ctttgttttc agaaatcaca tatatccagg ataaaagaaa catggaataa ataagtaaaa
    22681 ctacatatcc actaattgaa agtacgattg tctgttcttc taataattga ttaacaagaa
    22741 aataaataat gatcgttaac agactaagga acaagttctt accaaaatgt tgctttattc
    22801 cttgaaagaa aaaataaatg atatttcctg atccctcacc tttccattgc gccattacat
    22861 aaaaaacagc acatgtagca ggcacgatag taataacagg caaacacacg attacccaga
    22921 gaatacctaa taagatgtaa tcagaaacca caatcaactg tccataaagt ttagtctcaa
    22981 ctattttcat cttcctcact ctttcattcc agacatagct gttccttgaa caaaatattt
    23041 ctgtgcaaaa atataaacca ctagtaatgg cagcattgaa ataaccgttc cagccattac
    23101 aggtccataa aaagaaatgt tctgccctgt aaataaagct aaccctgctg gtaaagttct
    23161 catttttgaa ctacttgtca atatcaatgg ataaagcaag tcattccaac tattcatcag
    23221 cgtgaaaatc cctaatgata aaagagtagg tttgctcaat ggcaacatga ttttcaaaaa
    23281 aatattaaac tcacttaatc catcaatgcg tgctgcctct tccaaagaat ctggtaaagt
    23341 aataaaaaag gaacgcatca tgaagatacc aaatgcagtt gtcatacgag gaatgatcaa
    23401 acctgcataa gtatcaagca tgccaagcaa attgacttca ataaatagag gaatcatgaa
    23461 gacttggaat gggatcatca ttgtcagcaa aacgaaatag aacaaaactg attttccttt
    23521 gaaacgcata cgagcaaatg cataccccgc aagagaatca aagaatacag aagtgatcac
    23581 tgtccctcca gcaaaaatca ctgtattttt gatgtaatct aataaaggga tcgttgtcca
    23641 aaccttaata tagttatcta gggtatagct atctgcaaat attctaggcg gaaatgagat
    23701 aatgtctttt tcgtgtttaa aagaagaaat aatcagccac aaaaaaggaa acgcaataac
    23761 caacaatata actagcatac taatcgttcg aatgattttt ttaccactaa ctgcttttcg
    23821 actattcaca gtaactgtaa tcgagctttc tggtgccata caggtttccc tcctatttca
    23881 tttgtttttc tttccgtgac atgtacacat tagtggagat agctatagtt gcaacaatga
    23941 taaacaaaac cgaagacagt gctgatgcat agcccaattc ataaggtgct gtaaatcctc
    24001 gactgtaaat atattgaaca gctgtttctg tcttaaattg tgggccacca gcggttgcaa
    24061 catatacttg gtcaaacact tgcatcgaac caatcagatt cgttaaaata caaaaaccta
    24121 aggagggaac gatctgaggt agcgtaatgt taaaaaactg ctgtcgattt gttgcaccat
    24181 ccatttgagc agattcatac aaagagggag agattccctg aatagcagtc aagagaatcg
    24241 tcatagtaac accaaaattc ttccaaacag tcatgatggc taccgtcggc atagcaaggg
    24301 ttggatcgcg aaaaaattga gggatcggca agccgacttg tgaaaacaag taaggaatcg
    24361 cccctacatt cggatcaagc aacatagaaa aaataatcgc aattgacgtc aatgaacaaa
    24421 taactggaat gtaaaatgtc gaacgacaaa aacgattgaa tctggtattt ttagataatg
    24481 ctactgctgc taataaacca acgataattt gtgttggcgt ttcgagtacg gtgaaatata
    24541 aagaatgaat caacgaatta actgctcgtt catcactaaa tatacgtgtg aaattttcaa
    24601 aacttacaaa atttcttgat gtaaagaaaa tattcaaatc tgtaaaacta atagcaaatg
    24661 ttcctaaaag cggtatgaca gtaaataata ataaaactaa aaaagatgga gttaaaaaca
    24721 aatacgcagc acgatcagga cgattccaat atttggtgaa tccgttcata tagtccacct
    24781 catctttcat tttgataaaa tatacaatct tggtttaaac gaatctgaat aagatatccg
    24841 aacaatcgct gtgatttttg caataacacc tatctgcaaa tactaggtcg atcattcaga
    24901 tatctgtatc ttgccaacag tctatcctgt ataaagttct gtactatttt tgcagtaatt
    24961 gattgatggc atcatctgca tcgttcatga gtgtctcagg atcttctcct gctaataatt
    25021 tttcaatcag tggattgatg atatcgttat tgatcgcaga gatttgggta aaacctggta
    25081 aatatggctc tgcatagtcc atttgtttcg ataattcaga tacaatagga tcatttttta
    25141 cttcttcatc ttttgctact gaatccaagt aggcaggaaa accattttcg atactccatt
    25201 ttttaccaat ttcagttgta ttccaatatt tcaaaaactc atagacagct tcttttttat
    25261 tattatctgt acttgccgga atggcatatc ctacgccatc taaaatagct gtttttttgt
    25321 ctgaatcact cccttgtggt aaggtagtca cattataatc aatctcgttt gctttcaatc
    25381 cgttatttaa ccatggccca ttgatttcaa tggctagttg accagcattc attaaattgt
    25441 ccatttcggc gccagaaatc gattctggac cagtcttgtc tacatggatc attttttgga
    25501 tcgcttttaa cgttttaagg ttctcaggag acgcaaattc agacttagta taatcatcgt
    25561 tcactaattt accgccattt gctaacatcc agttatataa cggtgcagtt ccatccttat
    25621 tgaagacaaa acctgataca tttttactac tatcagttaa ttttggtgcc atttctgtca
    25681 attcttccca agtagtaggt ggttgttcag tatctaatcc agcttgttta aacaaatcat
    25741 tattccaata catatacatg ccttgtacct gcatggggac aaaatactgt ttaccttcta
    25801 cttcacctaa ttcaatagcc gtttctttaa aatcagattt atctactcca tcatattccc
    25861 agaaatcgtc taatggttgt accgctccgt tttctacata ctgtgcaaaa tctggataat
    25921 ttaatgcaat aaagtcaggt gcttttttag aagaaatcgc tgtaggaagt ttttcattca
    25981 aattggccca agtcatgata tccatttcaa ttttgatgtt tttatcattt gttttattaa
    26041 attcattaac gatttcttgc aatatttcac catcagatgc agtaaaccca ttccaaaaag
    26101 tcaaagtcac ttctttatca gaatcagatt ctgctgaact tccgccaccg caagctccta
    26161 aaatacctgt tgcagctacc aacaaagagc caattaacca ttttttacct ttcatttttt
    26221 gttcctcctt catctgataa acgacatctg accacattcc aagatagcgg tttcaataaa
    26281 caattgattt tcccatcttc agcttttaca tttgttgaat gacttggttt tacttgttca
    26341 cttttaggaa gattagttgc ttttgtatca aatccactca tttcagaaaa atcaatcact
    26401 ccctctaatg ataattcaga caagtgaaca gttagattca tttcttcttc tccacggtta
    26461 actgcaaata tgacaagttc tttgtcattt attacggcta tactttctaa atacggcact
    26521 tcttcaaaat cttccgtcgc atagtttgct gaacgaattt gtgggcttaa cacctctcct
    26581 tgtccataca cagcagcttg cataaatgga taatagatcg tctgtctcca agcttctccg
    26641 tttgtttctg tcctgattgg tgcaataaca tttaccaatt gagcaagaca agcaatcttt
    26701 actctatctg catgtttcaa taaagtgatt aatagacagc ctaatagtaa tgcatcttca
    26761 aaattatagt tatcttctag taaaggagga gcaatctgcc aaggcgctgc ttgctcatct
    26821 tgttccgctg aatgatacca aatattccat tcatcaaagg acaggttgat ctgcttctta
    26881 ctatgtttct tcgccttgac tgtatcgcac atcgctgtca cttcagaaat gaaccgatcc
    26941 atgtctaagg aacgtgcaag ataatgagaa agatcatttt tttggttgcc ataatattga
    27001 tgcagagaca aataatctat ttgatcgtac gcttgatcaa gaactgttaa ctcccaatca
    27061 ccaaatgtag gcatttgccg attcgaactt ccgcataaaa ccacctcaat tgaatcatct
    27121 acgagtttca tcgccttagc cgtttcctct gctaaccgtc catattcttc cgctgtttta
    27181 tgtccaatct gccaaggacc gtccatttca ttccctaaac accatgtctt aattccaaaa
    27241 gggctctctt gaccattcga acgccttaaa tcactccaat aagttccttt aggaaaatta
    27301 caatattcga ctaaattacg agctgcatct actccacgag tacccagatt gacagccata
    27361 ttgatttcgg cccccacatg ctgacaccag ttataaaatt catgaatccc aacttcattc
    27421 gtttcaattg tccgccaagc taaatccaac cttctaggtc tatttgctat gggcccaata
    27481 ctgtcttccc aacgaaatcc tgacacaaaa tttccaccag gatatctaat catgggaatg
    27541 ttcaattctt ttaccaagtg aatgacgtct tggcgaaatc cacttttatt agctgatgga
    27601 tgatctggct gataaatccc ttcatatact gctcgtccca tatgttcaat aaatgaactg
    27661 tacaaccgcg gatcaacttt gcctacacgc ttgacactac taatcaacat tgtggcttcc
    27721 ataactgacc tcctttaatt tatacatgaa taatacacct cgttgaaagc gttgtcaata
    27781 atataaatgt taaacttact tattttttat cgattttaat taacc
//
LOCUS       DACECZ010000024        27538 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.024, whole genome shotgun sequence.
ACCESSION   DACECZ010000024 DACECZ010000000
VERSION     DACECZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.024"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            103..549
                     /gene="nrdI"
                     /locus_tag="IWO35_000998"
     CDS             103..549
                     /gene="nrdI"
                     /locus_tag="IWO35_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ0316071.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(614..2596)
                     /locus_tag="IWO35_000999"
     CDS             complement(614..2596)
                     /locus_tag="IWO35_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0316072.1"
                     /translation="MAKNELTLEELAKHIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(2610..3506)
                     /gene="murQ"
                     /locus_tag="IWO35_001000"
     CDS             complement(2610..3506)
                     /gene="murQ"
                     /locus_tag="IWO35_001000"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0316073.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(3509..4570)
                     /locus_tag="IWO35_001001"
     CDS             complement(3509..4570)
                     /locus_tag="IWO35_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0316074.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(4781..5308)
                     /locus_tag="IWO35_001002"
     CDS             complement(4781..5308)
                     /locus_tag="IWO35_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0316075.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(5375..6877)
                     /locus_tag="IWO35_001003"
     CDS             complement(5375..6877)
                     /locus_tag="IWO35_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ0316076.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(7135..8256)
                     /locus_tag="IWO35_001004"
     CDS             complement(7135..8256)
                     /locus_tag="IWO35_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0316077.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            8382..9323
                     /locus_tag="IWO35_001005"
     CDS             8382..9323
                     /locus_tag="IWO35_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0316078.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(9473..10369)
                     /locus_tag="IWO35_001006"
     CDS             complement(9473..10369)
                     /locus_tag="IWO35_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ0316079.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            10638..11978
                     /locus_tag="IWO35_001007"
     CDS             10638..11978
                     /locus_tag="IWO35_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0316080.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            12196..12639
                     /locus_tag="IWO35_001008"
     CDS             12196..12639
                     /locus_tag="IWO35_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ0316081.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(13143..14561)
                     /gene="nhaC"
                     /locus_tag="IWO35_001009"
     CDS             complement(13143..14561)
                     /gene="nhaC"
                     /locus_tag="IWO35_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0316082.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(14964..16187)
                     /locus_tag="IWO35_001010"
     CDS             complement(14964..16187)
                     /locus_tag="IWO35_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ0316083.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(16218..17057)
                     /locus_tag="IWO35_001011"
     CDS             complement(16218..17057)
                     /locus_tag="IWO35_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ0316084.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(17074..17754)
                     /locus_tag="IWO35_001012"
     CDS             complement(17074..17754)
                     /locus_tag="IWO35_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0316085.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(17756..18829)
                     /locus_tag="IWO35_001013"
     CDS             complement(17756..18829)
                     /locus_tag="IWO35_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0316086.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(19476..20147)
                     /locus_tag="IWO35_001014"
     CDS             complement(19476..20147)
                     /locus_tag="IWO35_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0316087.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(20234..22912)
                     /gene="mgtA"
                     /locus_tag="IWO35_001015"
     CDS             complement(20234..22912)
                     /gene="mgtA"
                     /locus_tag="IWO35_001015"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ0316088.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTRFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            23571..24008
                     /gene="ndk"
                     /locus_tag="IWO35_001016"
     CDS             23571..24008
                     /gene="ndk"
                     /locus_tag="IWO35_001016"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ0316089.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(24030..24896)
                     /locus_tag="IWO35_001017"
     CDS             complement(24030..24896)
                     /locus_tag="IWO35_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0316090.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(25056..27149)
                     /locus_tag="IWO35_001018"
     CDS             complement(25056..27149)
                     /locus_tag="IWO35_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0316091.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         7873 a         5796 c         4852 g         9017 t
ORIGIN      
        1 tttctcatta tacaagcaaa gcgcctagtc cctttccaaa ttacaccacc aaaaaactta
       61 aaaaagatgc tatactaaaa ggaaagaatt ggaggatgat ccatgaacat tttatatata
      121 tcgatttcag gaaatacgcg tgcctttgcc aaacatctag cagaatatgc tgaaaaaatg
      181 catgcagaag atcctgtcaa tcctgaagtt actttaaaag aaatccatga aaatagtgat
      241 tttacgaaag aaacagaacc attttttact ttcgttccta cttatttaga cggcggaaat
      301 ggtttagata acggtgatac agaaatcttg accgaaacga tgcgtgaata tctggaacat
      361 gaagacaatc ataaactttg tcttggtgta gtcggtagcg gaaataaaaa cttcaataat
      421 cagtattgtc taacagccaa acaatatgca caaacatttg gctttccttt cttggcagat
      481 tatgaattac ggggaacacc atctgatgtg acacgaattt atcagatttt agcagaaaac
      541 aataaataat aaaaaagaag aagctgaaaa atagatttca gtttcttctt tcgtttctat
      601 tgtttctatc atattaagct ttgacatcca tcacaggtgc ttttgctttt gctttacctg
      661 ttttttccaa gacaaagttt tttacacgat ccatattggt taccaagacg atcatggatg
      721 tttctttgcc tgctgctttg atcgcttcta agtcagcttc tgctacaagc gtatcaggtg
      781 tcacttgctg cccttctgtt actttcacat caaatggcgt accgcctaag tcaactgtgt
      841 tgattcccat atgaagcaag atttctaaac catttggcgt tgtgatccca atagcgtgtt
      901 tcgttgggaa gacagagctg actaccccag taactggaga atagatatgt ccgttttctg
      961 gttctacaaa gtagccttca cccatcatct tttgtgcaaa aactggatca cttgctttat
     1021 ctaaagaaac aactgttccg tcagcaactg atgagaggat agtcacactt tcagctgaag
     1081 tttgaggtac tactttttca ggcacttcat ttactgtggc tgtttcagtc gctaattctt
     1141 ctttttctaa acggtcttta ggaataccga agaagaatgt agcaacgaag ccaccagcat
     1201 aagctgccaa taatccaagg atgtagccta accatttacc gtcagagatc aatgggatca
     1261 aagcggctcc tgaaggtcca atagcgatag ccccgacatt tccgatcatt ccgatcactg
     1321 ccccgccgat acctccaccg atacaagcag taataaatgg gcgtcctaga ggtaatgtta
     1381 ctccgtaaat caatggttcg ccgattccta ggatcccgac tggtaaagct cctttgatca
     1441 tttctgttaa ttctttatct tttttgcagc gaatccataa tgccaatgct gcacccactt
     1501 gtcctgcacc agccatcgca agaattggaa gcaatagcgt acgaccagtt tcatcaatca
     1561 tttgcacatg gattggagtc aaaatttgat gcagtccgaa cataaccatc ggcaagaata
     1621 atgtgcctag aacgaagccg gcaaatgctc caccaacgcc aagtacccag ttgatcccac
     1681 cgaccatgcc atcagaaatg aaacctgcta aaggcatgat gaagaaaatt tcaatcagac
     1741 cgatggcaat caatgaaaga gttggtgtaa cgatgatatc taccgcatca ggtactactt
     1801 tatgcagttt tttctcaacg attgacaata gccaaacagc gaagatgaca cctaggatcc
     1861 caccttgtcc agctgcaaga gcttcaccag taaataagtt tttcagtggt gcttccggat
     1921 tcatccctgt cagcatcaca acggccccga taactccacc aagagttggt gttgcaccaa
     1981 aaacttgtgc agcattgata cctgtataga taactaggta gctaaacatc ccgtttttca
     2041 agatgttcat cacatcgata tattgttgcc atgttgctgc atcaagtgtt ccggctgtga
     2101 ctaagtttgc taaaactgcg gcaatccctg ccacgatccc agtaccgaca aatgcaggaa
     2161 tcattggcac aaagatatta gaaatgtctt ttaataccga tttgatttta gatggttttt
     2221 gtttcgattt ttgtgccgct ttcatttctt gtgcccgttc gtttacacgg tcttttccag
     2281 atgctgatga atttgctgta gctgtactta cagaagaaag tttttctcct aatttcacgc
     2341 ctgccaaatc aaccatttgc tgtgctactt tgttcacttt cccaggtccg atgatgactt
     2401 gaagctgttc atcttctact acaccaagca ctcctggaat cgcttttaat tcagagacat
     2461 ttacaagatc atcattgatg atcttcatac gcacacgagt catgcagtgg acaatgcttt
     2521 ctacattgcc catgccgcct gcccctttat aaatgtgttt tgccagttct tccaaagtca
     2581 gttcgttttt tgccatggtc ttttccccct cataaggtcc cttttacaaa tccgtttgct
     2641 tcagctaatt ttgcttgagc ttcctcttta tcactatttg tcaaaatcat gacgatagcc
     2701 agcttaacat tttgatctgc ttcttcaaag tacttttcag ctgtttcgta atcagcttct
     2761 gtcgcttgca tgatgattcg ttttgagcgt tcgattagtt tttcatttgt tggtttgaca
     2821 tcgaccatca agttattgaa tactttacct ataccaatca tactgatagt agaaatcata
     2881 ttcaagataa gtttttgtgc tgttcctgac ttcaatcgag tagagcctgt caaaaattct
     2941 ggacctgcat cgacttcgat tgggaaatcc gcgtgtttac tgatttcggc ttgtttattg
     3001 caagaaatcg ttcctgtgac agcaccgatc gattttgcgt aatctaatcc accgatcaca
     3061 tatggtgtac gaccgctagc tgcgatacca ataactatat cattttttgt caaattcagc
     3121 ttgattagat cttcttgccc tagctcataa gaatcttcgg ccccttcgac agctacagtc
     3181 attgcttctt ctccaccagc aattagacct atgaccattt ccggttctac accgaatgtc
     3241 ggtacgcatt cagcagcatc taacacacct agtcttccac ttgttccggc tcccatataa
     3301 atcagacgtc cacctttttt aaaggactca atcgtgttag caatcactgg ttcgatactt
     3361 gtcagttctt tttcaaccgc agcggccact ttctgatctt ctttgttcat tagttgaacg
     3421 gcttgagaaa tactcatctc atctaatcca aaagtttctt ggtttctgcg ttccgttgtt
     3481 aggttctcta aattgatttt gctcattttt atcttccttc ttttcttata aattgatagt
     3541 tcataccagc tttgatttgg ttgatcaatg gtagctcttc tttgataact ttagctacta
     3601 cattcacttt ttcatccgct ggcaaatcat atttggttaa ttggatctca cccatatagc
     3661 gcaaatactt ttcgttatct aaagtaatag accctttcat acgcgtagct gtatttcttg
     3721 ctgggatagg cggaatctct cgaaaacgtg catctacgct tcgtatgaca tctcttgcat
     3781 cgtcttgccg attcgtatgc ttccctaata tatattcgta aaattcttca tctacaggtt
     3841 ccgtatggag caagactgtc tgttcttttt ggaaagaagc aaattgctcc tgaacgtttt
     3901 ctgacagacc tgcatctccg atgtaaacaa tatccgtgtt acttgctaat agatctagag
     3961 cgcctgcaag aggatggatc cctcgatgtt cttccaaagt tggcaatcct tgatatagtg
     4021 ggcctctcaa ctctgtatct cctggtacaa aaccttggac aaggagacct tgaattttga
     4081 gccattgatt ttttttctga taccattctt tgtccagccc tgtttcggga cgtggataat
     4141 aattatgcca agcttcaatc tgtgagaaat cagcttctgc ttctttcaat tcatcgatat
     4201 ctttaggcgt gatcgtgcta gcattcaaac tgattttcaa ttgatgtgac cagtcggcaa
     4261 tctgctgatt ggaaatatga taatccattc ttaaacctgt tacaccaatt gctttcaaag
     4321 gcgttagatc ttctaatgaa aaacccgctc gttccaatgc ctctcctgaa atatcgacca
     4381 tcaattctag tttattcttt ttcgcttcgc ttcctagttc gatcaatcgc ttttgatatg
     4441 ccgaaacatc atcttctggt atatgcatgg aagtgaaaat accgacaaag ccattggatg
     4501 ccatttggcg gatatactgt ttcttctctt ctgacaaaga atcgttcata aatattgaaa
     4561 agccaaacat attatccctc tttttctgta tccgtcttca tttctatgac tagagtatat
     4621 atctaaagaa acaaaatttc aatactttta ttaaaataat taaatttcat ttctaaaggt
     4681 tgagtttata ttatcaaaag aattgtcata acctaaaaaa ggcactgcta agcaaaagca
     4741 gtgccttgaa atattttatt taaataacac aaaaacataa ttactcataa gaaaccaatt
     4801 ctgtattatc taccatgaat gtaaagaacc cctcattagg catcggatga aaataagcat
     4861 tgatcactct tatcaaggca ttatggcaaa ctaacagata gacattttct tcgtcttcga
     4921 tgcattcttt caataaagga acgatccgtg catacacatc taagactgac tccccatttg
     4981 ggaatcgtac agcgaacgct aaacgtgctt tttgaaagtt ctcatctttg cttggcagtc
     5041 catcataatc cccaaaatcc atttctacca atcgttcatc aacggtcagc ggcaagctga
     5101 gacgatcagc aactgcttgt gctgtatctc ttgctcgttg aagaggggaa tggatgattt
     5161 tcgtgattgg aacaggcaaa tctactacct tttctgctag tttctgtgcc tgcatctgtc
     5221 ctttttcagt cagcggcaca tctgcccgtc cgcaaactaa tccttgtgcg ttccaagttg
     5281 tttctccgtg tctcgttaca taaagcatgt gattctctcc ttcgttggca aagttcccct
     5341 taaccatacc ttgttgttct tgagattaca agggttagat tctctcaaga gaaataatct
     5401 cagtagatac tttatccagg aaggtttgat catgttccac tagtaacatg gcaggtttca
     5461 cgttcaagat cagttgttct aattgttctt gattgaagac atccaaataa ttcaatggtt
     5521 catcccatgt atatagttca gctggctgtg ataaagattt agccaattcc acttttttcc
     5581 gttggcccat actcatctgc tcgatcttgt tatgaaaaac atctctttcc atcccaagct
     5641 ttcggaggtt gttcaaaaat gcttggtagt ctacttggtt tttctctgca aattccgcca
     5701 acgttcctcg attgtcttca taattttggc ttgcataact aatgctcaga tgttttggct
     5761 ggtatttttc tcctataacc ttgccgttga atgcccccag aagataatgg ataatggatg
     5821 actttcctgc accgttagga cctgaaatcg ccacccgctg atgaggctcg attgtaaaat
     5881 gaattggctc gaataacaga ttttcatacc ctaattgaag atcttctacg cttaaaagtc
     5941 gcttatggtg agacgcttgg ctattcatcg tcaaagaatc gatatattcg atatctttta
     6001 atagtttttc tttctcgctg atctgggtct ccatccgatt cacgattgct ttggatcgtt
     6061 tcatcgtccg tgcagcatca gcacccactg ctcctttatt cactcgtcta gattcagtat
     6121 cgatgaatcc gacttgtttc tttgttttat ctccttctcg ggaacgagac cattcggctt
     6181 tttcagctgc tgttttcttt agcctactga cttctttttt caatttttca ttttgagcca
     6241 tttcaaattc atcacgaagt tttttctgtt cttcatagat agagaaattc ccttgataaa
     6301 gttccagttg acttttttcg attgctaaaa catggtccac tacttcatcg ataaatcccc
     6361 ggtcatggct gaccacgatg aagccttgtt ttttcttttt caaataagcc gctacttgtt
     6421 ttctaccaga gatatccaaa tgattcgttg gttcatcgat taacgggaaa tgagtgtcat
     6481 ccacaaataa aagtgccagt aggacttttg tcttctctcc ccccgatagt gtgctgaatt
     6541 ctctccataa gatttcagga tctgtttgca tcaattggag ctctctttcg atttcccata
     6601 tctcaaaatc cgtaatatca tttaacacgt aataggttaa acgttcttta tcttttgtct
     6661 gttgcgggaa ataggcaaat tcttgctgat ggattacttg cccttgataa ggtagtttgt
     6721 tttgtagaat attcagtaaa gttgtctttc ctcgaccgtt tcgtccgata agtcctagtt
     6781 tccattgtgt gtcaaaattt agatttgctt gttcaaataa taatgtgcct tggctgtcgt
     6841 agccgaatgt cagatttttt atttcgattt tagacatttt ccatcacctc gtaaaaaaag
     6901 accagtccta ttttcagaac tggctaaaga ggatgcaagg gttggataca cacaaaaaga
     6961 cccatacatc gaaaaaacca agccaatccc gaaaatctgc acactgcttc cttgtctcca
     7021 caaggtacag tctgaatgca gctttcatca atcgacttaa tttttcaatg cacggatccc
     7081 tcactttgtc ctatatttga aattacgata gcataaagaa aatgattttg tcaactactt
     7141 cgggaggatg gtgaaaaatc tgctaagtgt ctgtcctaag atttttgttt tttctaatgt
     7201 ttgcctgtgt tccattgctt cttctaaacc tattggaccc aaatgaatac ttaagacaat
     7261 tgggagaaaa tcagaaagtc cttctagctc ttggtctcta cttccgcaaa tagcgattat
     7321 tggttttcga tatttttttg caagcactgc tacaccgtat ggaacttttc cgtggatcgt
     7381 ttgctgatcg atccgccctt ctcctgtaat gatgacatct gcttgttcga tctttttttc
     7441 caatccagtg atttggctga ctaaatcgaa accagaaacc atcttcgctc ccaacaaaaa
     7501 aagggctcca ccaataccgc ctgctgctcc acttcctgga atatcatcta aatccacctc
     7561 ccagaaatct tttatttgct gagcaatctt tcctgcttgt ttatctaaga aagctagcgt
     7621 cgtatctatt cctccttttt gtcttccaaa ttgccaagca gctccttgtt gacctgtata
     7681 tggattggta acatctgcag cgataatcaa ttttacttta gaaacaagaa cgtttcttcg
     7741 atcaaaatga agttttttag tagataataa aggatttcct tctgtgtacc cttctaaaga
     7801 agcacccaag gattggagta atcctagacc accatcgcta cacccgcttc ctcctaaagt
     7861 gacaataatt tgttctactt cttgaagcag tgcatcttta ataagttctc ctaaggcata
     7921 actggaagca agtcggatag tttgatccgt tggtttatca agtagatcta accccaatgc
     7981 ttgtgcagat tcgatgactg cggtgttttt tccttgccaa gttgtcaata aatagctgat
     8041 ttttcgctga cggaataaga gatccatccc atcaatggta cgccatgttc ccctgagcgc
     8101 gtaatagatt gcttctgcag tattttctcc gccatctgca atcggcacat tgatttttct
     8161 tcctttacga tcttcccagc catttagaac tgcctgattt agttcaaatg aagaagcact
     8221 tcctttaaat gaatcaattg ccgccaaaat atccatctgt atccctcact ttcttcttaa
     8281 gtaagacact ttttttcatt tttttcaaac aaaacaatgc tattttcttc catttttcta
     8341 tatactaaaa gggaactcat tgaataaagg agagaaaaaa catgaaaatc gcaatagctg
     8401 gggccggagc aatgggcagc cgaatcggat tgatgctaca tcaaagcgga aatgaggtcc
     8461 tcttgatcga tcgttggcct gctcacattg aagccattcg tacaaatggg ttgattgctg
     8521 actttaacgg taaagaagtg gtcgcaaaac tgccaatcta ttcaccagaa gaaatcatcg
     8581 aaagcaacga acacgtggac ttgatcgtag cattaacaaa agccaatcaa ttagatgata
     8641 tgttttgttc gatccaatcc attattactg acaatactta tgttttgtgt ttactaaatg
     8701 gattaggtca tgaagatgta ctggaaaaat atgtgccaaa gaaaaatatc ttatttggga
     8761 tcacgatgtg gacagctggt ctagctggtc ccggaaaagt cacattgtta ggtgatggag
     8821 aaatcgaact agagaacttg gaaccagaag gcgaagcatt tacaaaaaaa gttgtagaag
     8881 tctttcaaga agcaaattta aatccgatct atagccataa cgttcgttat tccatttggc
     8941 gaaaagcttg tgtaaatgga acattgaatg gtttatgcac gattttggat tgtaatattg
     9001 ctgaactagg cgcgcaaaaa gcggcagaga gcatggttcg aacaatcgta tctgaatttg
     9061 cttctatcgc agcaaaagaa ggaatcatac tagatcaaga agaagtgtac caacatattg
     9121 cttctactta tgatcctgac aacatcgggt tgcattaccc atctatgtac caagacctga
     9181 tcaaaaacca tcgtttgact gaaatcgact acatcaatgg tgccatttgg cgaaaaggac
     9241 aaaaatatga tattgctaca ccatattgtg cctttttgac ccaattagtc catgcaaaag
     9301 aaggaatcct tggcgcagaa taaaaggctc tctgatgaga atcagctgaa tcagcagctg
     9361 gttctctttt tttaagccca ataaaagatc aaaaagaaaa cacaactctc atgatgccat
     9421 aagaattgtg cttgtttgtt tcagtacata atctcttcaa aatgtaggtt ttttatttgt
     9481 tttcaacaga ggagaggtca tattcgtatt gctcataaat agtggaatcg ttagtgataa
     9541 atcgttttgc ccaattttct gcatcataaa attccaatag ctctgatagg attggcttaa
     9601 atggtgtatg cagaaattcc ggtaattcat ctaaatcacg ttctcgaagc aaaaaaatat
     9661 tttcaggacg ataaaaatga taatgagcaa tcgcttgatt atccgtcact agtttctttt
     9721 gaaaatacat ggattcaaat gtccgcaaag tcactccttg ctgaccctcc cgcaacagtt
     9781 ccagaatccc gcgcgattcc cctgtttttt tcaggtactc ttcataagta agatatccag
     9841 gttgttcatt ccctcgatgt ggaagaatga aatagttggt cgtaacatct ctttttataa
     9901 attcctgctt atagctttct gctcgttctt ttctcccatt attcgttgca ccaaaaaata
     9961 gatccgtctt tggttcgcta gaagaaaaat tcatatgttt cgaataaaat gaactattat
    10021 gtttcagccc aagttctttt gcttccaaaa aatcaaaatg ataaatctca tcgattgccg
    10081 gttcatcttg tgcctgcttc agcatcgcct cgtgttctgc tttgaagtga ttccaaaaaa
    10141 ataacacaat ttttgcattg gtaaatgggc gcacgcgtcg agccatctcc atatcaaatc
    10201 cataatctaa aaaaacgatc agctgataat ttgccaactg ttgtctagct gatttaatga
    10261 attccgactg tagtaaatct tttccggacc aaacatctaa agagcggatg gaaggcaaaa
    10321 aataaagtga tttatttttt gcaactagca gtacgtcatt tttgttcatc ttttccgcct
    10381 cactttctct gtcacttttt cctatgatag cctgtttaaa tagtaaaaag ccagtaaaac
    10441 gtttcctata tttgtatgaa aatttaacat ttgtatgaaa atatgatgaa ataaatcatt
    10501 cttctattaa aattcgccta aaagttttct gaatagttgt aattgtctga aaatttgtta
    10561 tcattagtca ttgttaaata ttgctattta ttcttaatat aaattttgat tgtgaattta
    10621 aaagaaagag gtcatttatg gaagataaaa aattgaaaaa acaacttagc tccagacata
    10681 tcaccatgtt agctttaggt ggagcaatcg gtgcaggtct atttaaagga agtggcgaag
    10741 cgattgggat cgccggccct tctgtattga ttgctttttt gattggtggg ctgattttat
    10801 atatcgtgat gaaaggtctg ggaaaaatcg ttttaagcgg tggcgacacg catcacggat
    10861 tatctggttt gatacgccct tatctaggtg ctcattctgc tgattttact gattgggtct
    10921 actggtctat gtggattatc aatatcatcg ctgaagctgt tgcagctgct tcctttttgc
    10981 agttgtggtt cccgcacatt ccagcctggg tatttgtttt tatgttggct gttttaacta
    11041 cgatcatcaa tctttattcg gtacggttat tcgcagaaac agaatattgg ctggcttttg
    11101 ctaaaatcag tgtcattatc ttactgatca tttttgctac ttatcttgtc ggtcagcaaa
    11161 tgttgggcac aggcgttttc cctacccttc agcaattgac tgaccatggt gggtttactc
    11221 ctcatggtat gaagggaatc atcagttctt tgcttgtagt tatctattct tacggcggtt
    11281 ctgaactgat tgctatcacg gtaagcgaag cagatgatcc taaacgtgcg attcctaaag
    11341 caatcaaagg tgtcatggga agaatcattt ccttttacat cattcctttg tttttgcttt
    11401 tgattctttt tccttggaat actttagcag gcacgaatgt cagtcctttt gtgatggtat
    11461 ttgaaaaaat gaatatccca tttgcagcgg atatcgtcaa ttttgtgatt gtccttgctt
    11521 tgttctcatc tatcaattcg ggtgtgtatg catcttcaag aattttgttc ttccgtttga
    11581 aggatcgaaa aggttcctct agaaaactag ctgttttaaa caagcatcaa gtaccgcaac
    11641 gtgctgtttt attttgtacc agtgttcttt atcttggtgt agctctttct tattttgtcg
    11701 gtgacaaatt atttggctat ctggcaggtt ccctttcgta tacagtatta ttgatctgga
    11761 tcattatcag tgcggcatcc tttgtactgg cactaagaaa aggaacatta tgggataaag
    11821 gaatcagctt ttttgcatta gctgtcttag gattgatttt ccttggtatc ctgttcacga
    11881 actccattgg ggtaactgta ttgactggcc tgctttacct gttcatttat ttcagttatc
    11941 aaaaaaagaa tgatgcgttc gtattgacga atgaatgaaa tcgatagaaa attttatttt
    12001 atttatgtgg aatagatgga gctattgaag aaaggagttg tgacaaaagt cttgttgcaa
    12061 ctcctttttc cgaataaaca gtctcatgac ctcttggtaa acggtgttca aaaaccctct
    12121 gtgcaaaata attgattgtt ctccatgctc atgacatgat agaagaaagg aatgaaaaaa
    12181 aggagtttgt tttaaatgaa aaagtattgg aagtttttgt tgatgatcgg tgtttcaacg
    12241 gtagttatgt ttggtatgat gtacttgaat gtctatgcgc tcgatcatat cttcttcagt
    12301 cgtacacgag tattcatggc gttgatgatg ggtgcagtca tggcagtcat catgctgttg
    12361 ttcatgtgga aaatgtatga aaacaaaaag ataaatgtta tgatcttagc cgctagtctt
    12421 cttgtttttg ccggttcctt gtttatggta cgaagccaaa ctactgtcaa cgacacagct
    12481 tggatgaagg ccatgattcc tcatcattct atcgctatcc tggcaagtaa acgagctgat
    12541 ttaaaagatc ctgaagtaaa aaaattagcg gaagaaatca ttcaggcaca agaagaagag
    12601 atcgacaaaa tgaaaaaaat gatcaaagaa atggaatgac cattatccgt ccaaataaaa
    12661 aaagaggatg ttccagaagt tatcagcttc aagaaataag aagaagctac ttctggaacg
    12721 ccgtgaataa ggggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    12781 aaatagcttc tcctattttt cgtttggtta ggcttattac cgcagatgca agcttttgaa
    12841 caccgtttat taagaggttg taaaatcagc gttctgacct gagtattaga gagacaaatg
    12901 gaaaaatagt tcctcatatt ttgccatttt ttgagctaat accgagggtc agctgattga
    12961 acaccgttta tcgagaggtt gtgaaaaagt cgcttagttt cgcgtattaa gaaaaatttt
    13021 gtgaaatggc tttccacatt tttgtataaa tttggcttaa taccgagaaa ctggcttttg
    13081 aataccgttt attcgtaaaa gggattgtga caaaagtctt gtcacaatcc ctttttatgt
    13141 ttttattgtt ctaattgcgc tgtcttgctt actgcttctt gcggtttcac aaattttttt
    13201 gagattcccc aacctgtgaa ggcactgacc atcgaaaaca caggagaaag caaactgaaa
    13261 aatacgaatg gcaagtaagt gagtgtagga ataccaaatg tacttgctac gaaacttccg
    13321 gcaattcccc aaggcactaa gtagttgatt actgttccac catcttctag cgttctacct
    13381 aatacagttg gatcaaatcc tgctttttca taagtttctt taaatgcatt accaggcaga
    13441 ataacagata ggaattgttc accaataaaa atatttactc cgatacttgt catcaatgtt
    13501 gtaaaaatca aatttggtgt gttcgtcatc tttcttgata ctcgttccat cacggttgag
    13561 atcaagccaa attcgatcaa caatccacct aatgacaaag ttagaataat caatgacaca
    13621 gtaggcatca tactttcgat tcctccgcgg ctaagcagtg aatcgatttg ttggtttcca
    13681 gtatttgaaa caaaccctga ttcgatgacc ccagccaact tcgttaagga agtttgtggg
    13741 ttttgaataa aaatcattcc tactgctaca gcgatattca acaaaatcgt taaaattgcc
    13801 ggcacctttt tccatgcaca cagtaacatt aaaaataaag gaatcagtga ccagattgaa
    13861 atagtaaagt ttgcttctag aacatcagtc acttcacgaa tagatgaaag acttgcggct
    13921 ttacttgtgt gtcctaaaat catgaataaa aagaaagaaa caacaaatgc cgggacagtt
    13981 gaccacatca tatgcttgat gtgtttgaac aaatcggcat caacaatagc tgctgctaga
    14041 tttgttgatt cggataatgg agacatctta tctccaaaaa cggcacctga aacaatagct
    14101 ccaacaacca gcgctggatt taagcctagt gttgtcccaa taccgaaaaa tgcaatccca
    14161 atcgttgaca taacagtaaa ggcactccct acagcgctac ctacgattgc acacacaaca
    14221 aatacagaag ggacaaacca ctggacactg atcaatttga acccaatgac cattagagtt
    14281 ggaatgatcc ctgcttgtat ccatacagca atcaatgaac cgactaaaat aaaaataaag
    14341 attgggatga ttcctggttt gatgccattg atgattcctt cgtgcagcca atctgtcggg
    14401 tatcttttta tcactgcata caacatgacc attccgatcg ctgctaagat cgtgatatta
    14461 ggtgagacct ttaatccgac aacgccaatc gcaatgccta aaacaataac cattaataca
    14521 agtgttgctt ctaaaaaact tggtgttcct ttttttccca tgataatctc tcctttttta
    14581 gttgttctta tgttctaaaa aagttaagat atcggattac tccgccgcaa ttcatccgcg
    14641 attcctcctt tataaaaata aaaagacctg ttttaaacta aggacgaatg actccgcggt
    14701 accaccttaa ttaaaaacag atctgttttt cactttcaaa cttttattca aatgtatgga
    14761 ctttccatgt aattcgtcaa ttaggcttga tcggttcaca gcgaccaccg actttctgaa
    14821 caaaacacct aaccgctact gcgtgaaaag ttgacttgta cgaagtttac cttccataag
    14881 atcatttgtc aatgaaaaag aaaatatttt ttcatttttc tgaaaattat ttttataact
    14941 ttcacacaca agtttatttt ttactagaaa gtagcgccct cttgtttttt tcttgctact
    15001 atttcttgat gttcttgctt gattctttct aatagctttg gctctttcaa cagatcaaga
    15061 gctgtattgg cgagtagttc tgctgcaatc gcaatagaag caaggccttt gtcactttta
    15121 gcagcagctt tgaattcttc tgtgtgtccg gcaatatatg tctcagagat tgctaccgta
    15181 ggctggattg ttggtatgac ttggctgaca tttcctacat cagaagaacc gagacttgtt
    15241 ctttgttccg tttctatttc ttcatctgga acacctgctt cttttgcatg ctggaagaat
    15301 aagtcatcaa aagaaggagt aacgatgaca tcatcaactg cattttggaa caatccaaac
    15361 tcaaaagtcg tgcccgtact tatcgcagca cctcgtacga tattttccac tttttcatat
    15421 actttatcta atgtttgccg atttcctgca cgtaaataga aacgtccagc agcgtaatct
    15481 ggaatgacgt tggcagctac tcctccgtct gtaatgatcc catggatatt gacatctttt
    15541 ggcaattgca agcgtagagc gttgattcca ttaaacactt ggatcaatgc ttccaaagca
    15601 tttactcctt tttcaggtgc tgctgctgca tgggaagcga caccgtaaaa tttgatgtcg
    15661 actggatcat tcgctaaaga ttctgtcgtt ttcccataac gaaaagctgg atggacgcat
    15721 aacgctacat cgacatcctc aaaaaaacct tctcgaacaa agctgccttt agctgatccg
    15781 ttctcgcccc cttcttcccc tggggtgcca tatacacgga tttctccacc agtttcatca
    15841 atgaattgct tcactatgct ggcggctaat acggagtaag taccaaacaa attatgcccg
    15901 catgcatgac caatacctgg taaagcatcg aattctgcca aaaaggcaat tgtgggaccg
    15961 ggtttattgg aaatataacg tgcatcaaag gctgttcggt gtccagctac ttctttttct
    16021 acccaaaacc tttctttttc taattgttta atcaacgctg ctgaggaata aaactcatag
    16081 ttacttactt caggatgttc gtggatatct agagcaagtg cttgatattc tggtaaacga
    16141 tattggatta gatccttcaa ttggtttgtt gagattgttg ttgtcataaa aaattcctcc
    16201 tatgctagtc atttttctta ttttctcccg aatgtttccc atgcaggcac gcttgatcct
    16261 tttgatgttt tctcgattac tttgactgtg tcttctgttt gataagcgtc tactattttt
    16321 tgatacgttt tattgttttc atcttctttt cttgcaacga tgatattcac ataaggacgt
    16381 gcatggtcat ccactggttc taagaagatg gcatcagaag caggattaaa tccagcatct
    16441 acagctacac cgctattgat cactgaaatg tctacatctg acaaagcacg tgctgtttgg
    16501 gaagcatcta attcagaaat gtctagattt tttctattct cggtaatatc agaaacagtg
    16561 ggcgcttgtc cttttgcctt atccactttg atcaatccag cagtttgcaa taacagaaga
    16621 gctctgccgc catttgtaac atcattaggg attgctactt ttgctccgtc tttgatatca
    16681 gctacatcct tcaaagtgtt agaataaatt cccagcggtg cattgacagt atttccgatc
    16741 gatactaaat cagttccgtg ttctttattg taattgtcta aaaagaattg atgttggaaa
    16801 gaattcaaat caatcgatcc atctgctaaa gccacatttg gttgattgta atctgtaaat
    16861 ttcacgtatt ccaaattaat gttctccttt ttcaaacggt ctttcacgtt gtcccataca
    16921 tctgtatctt ctccaataat ccctagtttt acgatagttt cttttttgtc agttgtatct
    16981 gcttttcctt ggctacaccc tgccaaccaa ataattgcta caaatgtaat gactacaaaa
    17041 cgaataattt ttttcatttt ttctcctcca ctttcaatgt gtaatttttt taatcagttg
    17101 attactgata aattgactta aaaaaactaa gattagaatc aataatgtag ccacaatcgt
    17161 tacatctgtt tggaatcgat tataccctct cgatatagca agattcccta atccgcctgc
    17221 acctactgct ccagccatgg tagtcagtcc gatcacgttg atgatagtca acgccgagac
    17281 ccgaatgata ccaggcaaac cttctaacaa atacacacgt aaaatgatac cgattggact
    17341 ggttcccatc gcttctgccg cttcgatcac cccaggatca acttctagca gtgcgttctc
    17401 gatttgacga gcaaaaaacg gaacaactcc aaaaatcaat ggcactaaag ctgctttcgc
    17461 accaatcgtc gttccaacta agaaacgagt agtcaatgct aaaagagaaa gcaagataat
    17521 gaaagggatg gaacgaatga tattgatcag cttatcaagt atttcaaata gcagacggtt
    17581 agctagtaat ccattagggc gtgtcactac taacgtcaca cctaagatga ttccaatcat
    17641 cccgccaatg aatgctgtcc aaaaagacat atatagtgtt tgcttcgtcg cttcgacaaa
    17701 ttcatccgga atcaaataaa cattgggcaa atatgtttta aagaaatcag tcattttatc
    17761 ttcctccttc gatcactttg atacgactat ctaaatcgtt tttttgtaaa atatttattt
    17821 ttacaccaga tttgatcaaa taatccaatg cttttttccg ctgtttggtt tctccagcta
    17881 aagtaacgat caaactccct aacggcgttt cttgtaataa ctctacattg ccatataaaa
    17941 tattcgctgt tacttgatat ttgctgtaca gatgagcgat tagaggttcg ttcgtctgat
    18001 ttccaatata acttaattcc accagccatt tattggcgat ctgatcggca aaagcagaat
    18061 gggcaatgat cgtttctaat gcttggtcaa gatgagtagc tgttcgaata aaatctttgg
    18121 tcaattcttc ttcaggattg ctgaagatct ggatgctgct acctttttca acgattcttc
    18181 catcttccat aaccgctacc ttgttgcaga tttctttgac aacttgcatc tcatgagtga
    18241 tcaggacgat tgttaaacct aactggcgat tcaatttttt taacaatgca aggatctgaa
    18301 gggtcgtttt cggatcaagt gcactggttg cttcatcaca taataatacc ttaggttcat
    18361 tcgccaaagc tcgtgcgatt gctactcgtt gtttttgtcc ccccgacagt tgtttaggat
    18421 aagcagatgc tttttcttcc aaaccgacaa gttccagtag ttcattcact ttttgacgac
    18481 gttcctgttt cgattttcca gaatacttta aagaaaaatc aacgttgtca aaaatagttc
    18541 ggctttccat tagattgaaa tgctgaaaga tcataccgat ggcctttctt tcttttcgca
    18601 attctttggg agagagctga ctcagattcg tctgattgac aatgacttct cctgctgttg
    18661 gtttctgcag caaattcatc acacgaacta acgtactttt ccctgcacct gagtaaccga
    18721 cgattccata aacatcgcct ttttcaatcg ttaaatctac agaatctact gcctgtatct
    18781 ctttttcttt ttgtttgaaa catacagaaa catctcgcaa ttcaatcatt aaaattcctc
    18841 cttatctaaa aaaagcgtcc tcaagaaaat gaaaatgtca ttttcttgag gacgcttttt
    18901 tgcgtgttac cacctcatgt tcaagctgtg ttcacacaca actcctcttc aagtacgatc
    18961 atactctagc aagatatcgg tcgcagccgt catcgcctta catctatgct cgggatgaat
    19021 tgctcgatgg ccattttcca atagtcgttc cttgcttctt ctcagctacc gaagctctct
    19081 gtcaaaggtt cttctatcgt acttaccatt tcaatgcaaa taatattcaa aaaaaaagtc
    19141 cttttactaa cataaactat gtcagtaaaa ggacgacgta tcgcggtacc accttttttc
    19201 ggaagtttcc ttccctcaag cccaacatct caaaaagaga gattggcgtt cgttttaacg
    19261 gtcgaacagt tccgaatttg cctacttatc gacaaattat tctccaaggc cattttcagc
    19321 ttttcttact tgactcttct caccagccag agcctctctg caaagtttcc aaacttactt
    19381 tccttttcaa cgaaatcgat attttgttgt tgggattaat ctacatgagg aaaactgaaa
    19441 agtcaagtac gaaattcaaa ttattttttc tatatttatt tttctgtttt tttatttaac
    19501 atttcaataa cagttcgaag atcttttcct tccgcaagaa gtttcaattt ttccttttct
    19561 tcgacggtat aatttcggac aaaactggaa tcaggtatca aaacaacgcc taatcctgct
    19621 cgtgaagcag ctgttgctcc tactaaagaa tcttccactg caaaggctga actagcttct
    19681 aatcctgttc gttccaaagc tgtcagataa ggaatcggtg aaggcttgtt ctccgacact
    19741 tcgtctccga atgtaaacgt gtccaagtaa tcagctaatt taaagtgatc caagatatct
    19801 gttgctcttt tcgtaacagt ggtcgtagca atgcctagtt tcatatgacg ttcttttaag
    19861 tctgccaata tttcttttgc atatggtttt aagtggatgg ctccgcttgt cagctgttga
    19921 taaaaatatt cttcacgctt cgctcggact tcctgtactt tttcagggct acctgctgct
    19981 ttcactaaaa tatcaaaact ttgtttccag ttttttccgc cccaagcgtc aacgatttct
    20041 tttgggatat ggatatcaaa ttgctgaaat ccccaaagcc agccatcacg atacgttttt
    20101 tctgtgtcaa ctaacaaacc atctaaatca aatattgcac ctttcatcta tacattaatc
    20161 ctttccaaaa caatacacat agtttagcac gactggtcca aaaagaacag aagcaaagaa
    20221 ccagccttct cgtttataag agttcttgga atcgacggat atagattttc ttcacagctg
    20281 tcacaagcgc cagataagcg atgactgtag caaataacca taaccagtaa ttgccaggaa
    20341 gtggcatcaa gcctaattca gcaccaaacc ttgtaaaagg aaggacagaa ccgacaataa
    20401 ttcctaatgt agtgataaca aacatggcaa atgttgcgtt gctttggata aatggtactt
    20461 tcggtgtacg cagtgcatgg agcactaagg tttgtgtcca tagtgattcg acaaaccatc
    20521 ctgcatggaa taatgcgatg aaaatcactc gctgtgaact atccaatgta tggaaacttc
    20581 cgcccactac agcaggacaa ataacaaaat acatcaacaa gtaagtagta atatcaaata
    20641 tcgaacttgt tggaccaaag taaaccatga attccccaat actagatgca ttccattttt
    20701 ttggctcgtc caaatattct ttatccatcc gatcccatgg caaggaaatg caggaaacat
    20761 catagatcag attcaaaaac aaaagttgta aaggcagcat tggtaaaaat ggcaggaacg
    20821 tacttgcaat cagcacggaa aacatattgc caaaattaga acttgctgta gccttgatat
    20881 atttcatgat attcccaaaa gtttctcgtc cagaaagaag gcctctttcc aaaatcgtca
    20941 aatctttttc taaaaggatc acgtctgcag attctttcgc aatatccaca gctgtatcta
    21001 cagaaatacc gatatcagcc actttcattg caggagcgtc attgatacca tctcctaaaa
    21061 atccgacagt atgaccattt ttacgtaaag ctgtcgtcaa acgtgctttt tgctgaggat
    21121 tcaattttac gaatacttca tggtcttcag ctacttgtgc taattcatga tcactcattt
    21181 ccgagatttt ctcaccggta accaattcat tgatctctag acctacttga cggcacactg
    21241 aacgtgtaac taattcgttg tccccagtca gaacttttac agctacacca tgttctttca
    21301 gagctttcaa cgcttgtttc gttgtttctt ttggtggatc aaggaaagca agataaccaa
    21361 tcaaaaccat gtctgactca tctttgactg aaaattctcc aacgacgctt ggattggtct
    21421 tttgtgcgac accaatcact cgtaagccat cttcattaag tgactgtact tttgcaagta
    21481 cggcttcttt tcgttctttt gttaatggtg taacagtacc gccaagatca ataaatttag
    21541 aaatgttcag catttcttca atcgcgcctt ttgtgatcat ttgtgtttta ccagctggat
    21601 cagcaaccac aacactcata cgacgacgtt caaaatcaaa aggaatttca tccacttttt
    21661 gataattgat cttatctgta tctaaagttt ctgttgcttc atcaatgata gccacatcta
    21721 acaaattttt cagccctgtt tgatagtagc tatttagata cgcatggcgc aaaacacgat
    21781 catcttcatt tccttcacaa tccatatgat attcaagaat gattttatct tgtgtcaatg
    21841 tccccgtttt atccgtacat aacacatcga ttgcaccaaa gttctggatg gcattcaaat
    21901 ttttgatcac tgttcctttt ttggccatca cacttgctcc tttgaccaaa ttcgtcgtaa
    21961 cgatcattgg aagcatttca ggtgttagac caactgctac agatagtcca aaaagaaaag
    22021 cttctagcca atcccctttt gtaagcccat tgatgacgat aactgctgga gccatcaatg
    22081 acataaattt gatcagtagc atggatgttt tatggatgcc taattcaaag cttgattgaa
    22141 ttggtttttc agatagcgtt tctgctacat gaccaaatag tgtttcattt ccaacagaaa
    22201 caatgattcc ttccgcactt ccactgatga cattacttcc cataaagaca agattttcgt
    22261 aacttgtttc gctttgctga tcgtcttcac atactttcgc atgcttttct actggatagc
    22321 tttcccctgt caatgctgct tgggaaatga ataaatcctt tgcggacaat aaacgcatat
    22381 ccgctggtat catatcccct gctgaaagcc gaacaagatc tccacagacg atttcttcca
    22441 taggaatctc tgtgtacttt ccattacgtt ttacagctgc cgttactttc acaagtgatt
    22501 tcagtttttc agccgcttga ttggaacgta cggactgaat caacgtcatt gtcccgctga
    22561 cgataaccat agtgaagata atggctacac cgaaaagatc tttatctcca ggagctgcta
    22621 atacatattc cgtgatgaat gaaataattc ctaatgcgat caacactaat gtaaatggcg
    22681 tgatgtatgc tttcaatatt tctacaatga atggtgtttt tttgccatat gaaatcgcat
    22741 tttcaccgta attttctctt gaacgttcga tttgttcttc tgtcaatcca tttgctgatg
    22801 catggaatag ttcaaataat tcttgtttcg tttttccagc aaaagctgca tagatttttt
    22861 gtgtatcttt ctttttttcc atctcaatct ctcctttgtg tccaaaagac agctgttctc
    22921 gctgcttttg gtctttagct attcatcgga tcgatagaga aaaaactgaa aggggctgag
    22981 aacagataga taatagccct ggcctttcct ctagatcctt gtttttcgaa tgactgtcat
    23041 ccatttgttc tcatctcctt tactaagtaa ttttaattca tgacttccaa tcatttttcg
    23101 aatagttatt tttccaatta gaccacctcc tttttatttt tcaccgatgg ggatttgcgg
    23161 caacaaaaaa acctctcgcc aaatactcag cgaaaggttt gaaaaagcat attaaaaaaa
    23221 gcttctcaga tctcatcgtt gagttttgac actgtacaac gtaaaaagca gatacctgct
    23281 tttagctacc ttatgaaaag ccttatctcg acaattcctg ttctacccat tggcgtctct
    23341 cgacgttttt gggcagtagc ctatgtttgt ataggagcct cacctaacaa atcttattcg
    23401 gttttccttc gttaatataa caactaaaaa agaggatgtc aaatcaattt tccatgtttt
    23461 tcatactttt tttaaaatac acacgttata ttcctgtctc acgcggtttt atttgtctca
    23521 ttttccacct cccgctataa tagaaatata gtaggaaacg aggtgctgtt atggaaagaa
    23581 cacttgttat tatcaagcca gacggcgtga gacgccattt agtcggaagc atcatccaac
    23641 gttttgaagc aaagggactg gccattgctg aaatgaagtt tgaaactatg actcctgaat
    23701 tagcaaaaga acattatcag cacttgactg agcgcagttt ttttgatgaa ctgatcgatt
    23761 acatgacttc cggtcctgtc gtttatcttg tgctggtcgg cgaggaagtg atcgatatcg
    23821 tccgtaagat ggttggagca acaaaagctg ccgatgctgt tccaggtacg atccgaggag
    23881 actacgcgct gccggggact gaaaacatca ttcacgcttc tgattcccgt gatgctgcgg
    23941 taaaagagat cgcccgcttt ttcccgccat tcgatataaa caaaccgtct gaaaaagcgg
    24001 cgaagtgata caaattctaa actgctggtc tagttgctgg gttcagcaat ggccagcagt
    24061 tttttctcta aggacctccg ttgattcttt cctaacatat attcgagcac tgtgaagaat
    24121 tcttggtagt ctttcgtctt ttcttcccct aaagcagcag aaatcaatct ttgataataa
    24181 tctgccatca atgtttcata gatcactgta cttctttctt tatgtgctaa gctccgtttc
    24241 atatcttcat tcacactacg tgcctttttc aaccgaccat tttctatata aaacgaagtc
    24301 aggttcatta aaaaagtaaa gagatactgc tcatgattgt tcaactgtgc taatcgtcgc
    24361 attctttttt tgattttcaa ataattgttc tcaatgaacc gatcactgaa tatccaagga
    24421 aaatcagcta gaatagatag ttcaaattga ccatagtctg gtatctgcat caagtattct
    24481 tcaatgattc ggcattcata acgtaaaaat tcaaattctg tctcagatac ttctgttttt
    24541 tcgaccatgt gaagattcaa aaccaaataa tacagatctt ctgttttctt aaaatgccta
    24601 cggttattgc tgatctcttt ataaggatca aagctgtctt cttgtacccg atctagcatt
    24661 cgtctttttt tctttaactt tattggttca tccagctggt ttcgcatcca ttcatattct
    24721 gcaagtgtca tgttcattcg ctctaaaaat gatctcaatg ttacaaatga catttttttc
    24781 actgaatttg tctcaatccc gactagagaa gtcttaaaac agattccttc cgctaattgg
    24841 gactgtgtca acttcttgtt gactcgaagt ttcttgatca attcaccatc atacataata
    24901 gttctccttt ttaatttaaa tttttccaca ttcaataaat agatacgtta aaaaggaaaa
    24961 atacacgaaa aaaataaaaa aatgtttttt tcataaaaaa taccaacctt caagcattag
    25021 aatgttgtct aactactgaa ggttggtttg attgtttatt gattattttt ttgctgttca
    25081 acgtctgttc ttgttactgg atataagttg gttgttgttt tattaccatt ttttgaaaag
    25141 acacttgtcg aatgttcgcc tttttgcttt tcgattgctt cattacgttc cgcttcattt
    25201 ttatagtcat attttttcgg atcaatcgtc tcaaatccgt ttggcacata aaaccgcaag
    25261 agattttcct gattcagctt atctgaaaga cttaacgcag tcttgacttt ttcctgatct
    25321 ttggagattt cctcatcgat tcctgctgct ttcgtatcga tgatttctcc tgtctgatta
    25381 ttgtaagctt ttcctcctag gacggtgtat ttgggtgtca caaagttccc atttcgaaaa
    25441 gcaactactt gatcatgctg agaagataat aagtctgtac caaaatgaag ataatcttta
    25501 tcatcgattc ccaatagatg tagtaatgtc ggcaaaacat cgatttctcc accgtattca
    25561 tgattgactg tccctttagt ataaccaggc atatggatca tcaatggcac tcgttgcatc
    25621 tgtgcgttgt caaattcatc ccatgtatct ggatctttac caagagcgct tgctaaatct
    25681 ttattatctg tattcgagat cccaaaatga tccccgtaaa tcacaaagat tgaattttgg
    25741 taaataccag attttttcaa atacgtaaaa aattgttcca atgattgatc cagataatgt
    25801 gccgttcgaa cataattatc tactgtgcta ttgccagtat tcaaagatgg aaagtcgaaa
    25861 ttcttgtcat ccgtataata aggcgtgtgg ttggttacgg aaagaaattt cgtgtaaaac
    25921 ggctgttgta gatgctcaag atattgggca gattctctga ataaatcttt atctaatatc
    25981 ccataaccta acgtttcgtc ggattcatca aaataagatc gatcaaagaa attgtcataa
    26041 cctaagtttt tatatacatg atctctattc caaaaactag ctacattccc atggaaaaca
    26101 gcgcttgtat agccttgctt ttgtccaagg atcgctggtg cggcttgaaa gacattgtct
    26161 gacccaagtt ctgtgaataa tgagccttga ggtaaaccaa atgtgccagt ttccaacata
    26221 ttttcagcat cacttgtctt tccctgtcct acttcatgaa agaaattgtc aaagctgatc
    26281 gttgcttgat tctggaaaat actatttaaa aacggtgtga cttcttgtcc gtctaccttc
    26341 atattaatca aaaactgctg gaaactttct aaatggagaa cgatcacatt tttcccttta
    26401 gcgatcccaa atgtttcggg atttggctct gcataatgtt tctttgtata atctaaaact
    26461 tcatcaatcc cattactgct tgcatgcgcg cgaacactgc tagtcattcc tgttttgatt
    26521 ccgtcataaa tagtatacgc atcgattcct agatatttga caatatatga acggtcaaat
    26581 gttcgctgca gcaattgtgg acggttggct tcgctcagcg ctagatttcc tgaaaaaata
    26641 agccctgaaa gacaggtaac agcgatagcc atcggtttgc cgacagggtt gcttttgata
    26701 ggtattttct tgaccttcaa ccaaaccagt agcccaatga acacagcgat atccagccag
    26761 tagatgacat catggggctt catcaaagaa aaggagcttc ctgacaatcc ttgcgacact
    26821 ttcgaaaagc ctaatacaga tttgactgta atgaaatctg tgaattcccg ataaaaaata
    26881 atattcaagt acaacaaaag cgtatttaaa atatcaatga tcaaaagaat agctaacgct
    26941 ggctttggct ttttaatata caaagaaagg ccaaaaaaca ataaggtagt cgcaattggg
    27001 ttgatccaca agataaagta ctggatcgta ccttccacac ctaatgaaaa atctgcatag
    27061 taggcaatga tcgtcttcag ccagaaaaag aaaacagctg tcaaaacgat tgctaaacga
    27121 ttttggcaca attttttaag ttgtgtcacg tatatcactt ctctctattc acattttttc
    27181 ttattttgct cctgaaaaat gaaaatttcc taaaaaagtt gttatcttaa gattttttta
    27241 ttctttctaa agattattcc gtccactttt cacagcataa aaccgctgcg atgtaagcga
    27301 ttaaactaat gaagagataa aagtcttgtg gtgaccgctt cactccacgg tattcaaaag
    27361 ccagtttctc ggtattcgta caaaaaaaga ggctgtgaca aaattcttgt cgcagctcct
    27421 tatttcttga tgcaggacag ctttttcaca acttcttcct taaacggtgt tctcctgcca
    27481 actcctcggc cacaagtcac aatatttata aaacatgaga agctgttttt tgaaattg
//
LOCUS       DACECZ010000025        26998 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.025, whole genome shotgun sequence.
ACCESSION   DACECZ010000025 DACECZ010000000
VERSION     DACECZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.025"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            44..901
                     /locus_tag="IWO35_001019"
     CDS             44..901
                     /locus_tag="IWO35_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ0316092.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1016..1936
                     /gene="pstC"
                     /locus_tag="IWO35_001020"
     CDS             1016..1936
                     /gene="pstC"
                     /locus_tag="IWO35_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ0316093.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1936..2820
                     /gene="pstA"
                     /locus_tag="IWO35_001021"
     CDS             1936..2820
                     /gene="pstA"
                     /locus_tag="IWO35_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ0316094.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2835..3641
                     /gene="pstB"
                     /locus_tag="IWO35_001022"
     CDS             2835..3641
                     /gene="pstB"
                     /locus_tag="IWO35_001022"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0316095.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3657..4415
                     /locus_tag="IWO35_001023"
     CDS             3657..4415
                     /locus_tag="IWO35_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0316096.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4428..5105
                     /gene="phoU"
                     /locus_tag="IWO35_001024"
     CDS             4428..5105
                     /gene="phoU"
                     /locus_tag="IWO35_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ0316097.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5356..6374
                     /locus_tag="IWO35_001025"
                     /pseudo
     CDS             5356..6374
                     /locus_tag="IWO35_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723340.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
     gene            6371..7138
                     /locus_tag="IWO35_001026"
     CDS             6371..7138
                     /locus_tag="IWO35_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0316098.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7238..8806
                     /gene="liaX"
                     /locus_tag="IWO35_001027"
     CDS             7238..8806
                     /gene="liaX"
                     /locus_tag="IWO35_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ0316099.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9106..9423
                     /locus_tag="IWO35_001028"
     CDS             9106..9423
                     /locus_tag="IWO35_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ0316100.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            9423..9773
                     /locus_tag="IWO35_001029"
     CDS             9423..9773
                     /locus_tag="IWO35_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ0316101.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9940..10878
                     /locus_tag="IWO35_001030"
     CDS             9940..10878
                     /locus_tag="IWO35_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ0316102.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10893..11723
                     /locus_tag="IWO35_001031"
     CDS             10893..11723
                     /locus_tag="IWO35_001031"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ0316103.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11767..12792
                     /locus_tag="IWO35_001032"
     CDS             11767..12792
                     /locus_tag="IWO35_001032"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0316104.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12807..13745
                     /gene="galU"
                     /locus_tag="IWO35_001033"
     CDS             12807..13745
                     /gene="galU"
                     /locus_tag="IWO35_001033"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0316105.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13870..14310
                     /locus_tag="IWO35_001034"
     CDS             13870..14310
                     /locus_tag="IWO35_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ0316106.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKATAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14313..14915
                     /locus_tag="IWO35_001035"
     CDS             14313..14915
                     /locus_tag="IWO35_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0316107.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14974..16080)
                     /locus_tag="IWO35_001036"
     CDS             complement(14974..16080)
                     /locus_tag="IWO35_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0316108.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16290..17291
                     /gene="ccpA"
                     /locus_tag="IWO35_001037"
     CDS             16290..17291
                     /gene="ccpA"
                     /locus_tag="IWO35_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ0316109.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17628..19973)
                     /locus_tag="IWO35_001038"
     CDS             complement(17628..19973)
                     /locus_tag="IWO35_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0316110.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSISDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLSKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20252..21154
                     /locus_tag="IWO35_001039"
     CDS             20252..21154
                     /locus_tag="IWO35_001039"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ0316111.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTAAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            21367..23142
                     /locus_tag="IWO35_001040"
     CDS             21367..23142
                     /locus_tag="IWO35_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ0316112.1"
                     /translation="MLNTLKPLINQLTKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHFSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(23208..24794)
                     /locus_tag="IWO35_001041"
     CDS             complement(23208..24794)
                     /locus_tag="IWO35_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ0316113.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            25045..25269
                     /locus_tag="IWO35_001042"
     CDS             25045..25269
                     /locus_tag="IWO35_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ0316114.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            25411..>26998
                     /locus_tag="IWO35_001043"
     CDS             25411..>26998
                     /locus_tag="IWO35_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0316115.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIAMIPLPLLAITSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYLKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVKHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLMLDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAA"
BASE COUNT         8636 a         4810 c         5657 g         7895 t
ORIGIN      
        1 aaatgataaa ataaacatac tttaggatta aaggagattt ctaatgaaaa aacttaaatt
       61 attcttacca ataataggat tagccattct tttatcaggc tgttcaaagt ggattgatcg
      121 aggagaatcc attacggctg ttggctcttc tgctttacag cctttagtag aaacagttgc
      181 tgaaacttac cagacagaaa atcctggaaa attcatcaat gtacaagggg gcggaagcgg
      241 aactggtttg tctcaagtac agtcaggagc agtcgacatc ggaaactctg atttgtttgc
      301 ggaagaaaaa tcggggatcg atgcctcagc attagtcgat catcgagtag cagttgttgg
      361 gatcactcct attgtgaaca aggatattgg cgtaaaagac atcagtatgg aaaacctgaa
      421 aaaactattt acaggcgaga tcaaaaactg gagtgaagtc ggtggaaaag atcagccagt
      481 agttatattg aaccgcgctt caggaagcgg aacccgtagc acgtttgaaa aatgggtatt
      541 ggacggagaa acagctgttc aggcacaaga acaagattca agcggaatgg tgcgccagat
      601 cgttgccgac acacccggtg caatcagcta tgttgctttt tcttatgttt ccgatgatgt
      661 agatacgttg aatgttaacg gtgtagaacc gacagatgaa aatgtgacaa cgaacaaatg
      721 gacgatttgg gcatatgagc atatgtacac aagagatcat ccaaaaaaac tgacaaaaga
      781 atttttagac tatatcttat ctgatgaagt acaagataac atcgttggag aactaggtta
      841 tatccctgtt tctcatatga aagtcgaacg tgattggcaa ggaaatatcg ttaaagagta
      901 atctttacac aaaatttaca aacttagcta aacttttaca tgttattaaa accaaattga
      961 tgttcaattt gtacactgag tgagtatgtt aaacctgatt aagggggagt catctgtgga
     1021 agatgtgaaa aaagtattat taacaaaatc aaaacgttca aaaatggaac agcgtggaaa
     1081 attcatcagt tttttatgta tcgcgctgat cgtgttggtt gtattatcta ttttttattt
     1141 tgtagctagt aaaggattgg ccaccttttt tactgataag atcaatgtat ttgattttct
     1201 gtttggtaca gattggaatc caagccaaat cggagcaaat gggaaaccaa tggtaggggc
     1261 attgcctatg atcacaggtt cgtttattgt gaccttttta tctgctatcg ttgccacgcc
     1321 atttgccatt ggtgcagcag tattcatgac ggagatctct cctaaaaacg gaacgaagat
     1381 cttacagccg gtcatcgagc tgcttgtagg tattccttct gtcgtttatg gttttatcgg
     1441 tttatcagtt atcgtgcctt ttgtcagaag tatttttggc gggacagggt ttggtatctt
     1501 agctggtaca tttgtgttgt tcgttatgat cttgccgact gtcacaacga tgactgttga
     1561 tgcattaaag gcagtgccac ggcactatcg ggaagcttca ttagcgcttg gtgccacaag
     1621 atggcaaaca atctacaaag tcgtgctacg agcagccgtg cccgggattt taacagcagt
     1681 cgtttttggt atggctcgtg cgtttggtga agccttagct atccaaatgg tcatcggtaa
     1741 tgcagcattg atgccaacag gacttactac tcctgcttct acattgacgt ccatcttaac
     1801 aatgggaatc ggaaatacaa tcatgggaac agttgaaaat aatgtgttat ggtcattagc
     1861 gctgatcttg ctattgatgt cattattctt taatatcgtg atccgtatga ttgggaagaa
     1921 aggagagctg aaataatgaa cgcaaaacgc tcagataaaa ttgctacagg tgttttatat
     1981 atagtttcgg ggatcatcgt attgatttta gcagctttac tactttatat cttagttcgg
     2041 ggaataccgc atatttcatg gtccttcttg accgaacctt ccaaagccta ccaagaaggc
     2101 ggtggaatcg ggatccagct atttaactcg atttatttgc ttttgatcac gatgatcatc
     2161 agttttccta tttctttagg tgcgggtatc tatctttcag aatatgctgg aaaaaactgg
     2221 ctaacagatg tgattcgcac gtcgattgaa atcttgagct cgttgccttc tgtcgttgtc
     2281 ggtttattcg gtttcttggt tttcgttatt caattccagt atggtttctc gattatttct
     2341 ggtgcattgg ctttgactgt attcaacttg cctcttttga ctagaacagt tgaggagtct
     2401 ttacaagcgg tccattacac acaacgagaa gcaggattgg ccttaggttt gtcacgctgg
     2461 gaaacagtga taaaagtggt ggttccagaa gcgacaccag ggattttgac tggtgtgatc
     2521 ctaagttctg gaagaatttt tggtgaagca gctgcattga tctatacggc tggacaaagt
     2581 gcacctgcgt tagattttac gaactggaat ccattaagtg tttcaagccc gatcagcatt
     2641 ttccgtcaag ctgaaacatt agctgtccat atttggaaga tcaatacaga aggcacgatg
     2701 cctgatggtg tttctgtttc ggctggcgct tctgccgtgt tgatcattgc tgttttactg
     2761 ttcaacttcg gcgctcgtgc gattggcaaa cgattatacc gaaaaatgac ttcagcctaa
     2821 ggagaagaag caaaatgaaa aaatataact ggaatgaaac aaatgtcatc accatgccaa
     2881 aagagaaaga agtagcacta tatacggatg atctacatgt ctggtatggc gacaatgaag
     2941 caatcaaagg agtggatctg gaatttgaaa aaaataagat cactgcactg attggtcctt
     3001 ccggctgcgg aaaatctacg tatcttcgtt cgttgaatcg aatgaatgat gggatcgcta
     3061 atacgaaaat cactgggaaa atcatgtatc aaggcgtaga tattaatgcg cctcaagtgg
     3121 atgtttatga gatgcgcaaa cggattggca tggtttttca acgtcctaat ccgttcagca
     3181 agtcgattta tgacaatatt acatttgctt tgaaacagca cggcgaaaaa aacaaaaaga
     3241 aattagatga aatcgttgaa acaagcttga aacaagctgc cttgtgggac caagtaaaag
     3301 acagcttgga caaaagtgcc ttggctttat ctggcgggca acagcaacgt ttatgtattg
     3361 ctcgtgcgat cgcgatgaaa cctgatatct tgctgctaga cgagccggca agtgctttgg
     3421 accctatttc gacaggaacg gttgaagaaa cacttgtaaa cttgaaagaa gactatacga
     3481 tcatcatcgt gacgcataac atgcaacaag cagctcgtat cagcgactat actgcctttt
     3541 tctatctagg aaaagtaatg gaatatgaca aaactaacaa gatatttacc agaccaaaaa
     3601 ttcaggcaac cgaagattat gtgtccggtc attttggtta agacggaggc ctcaacatga
     3661 caaaagaaat tatttcatct aaagatcttc atctgtatta tggaaagaaa gaagcactaa
     3721 aagggatcaa tctaagtttc aatcaagggg aaatcactgc gatgatcggt ccttctggtt
     3781 gcgggaaatc cacttatctg cgttctttga accggatgaa tgacctgatt ccgaatgtaa
     3841 cgatcaccgg aagtgtttta tataaagaca aagacatcta tggtccaaaa acagatgttg
     3901 ttgaactaag aaaagaaatc ggcatggtat tccagcagcc aaacccattt cctttttcta
     3961 tctatgaaaa tgtcatctat ggattgaaat taaaagggga aaaagataaa aaagtccttg
     4021 atcaagtagt tgaagaaagc ctgaaggcag cgtctgtttg ggatgatgtg aaagacaaat
     4081 tgcataaaag tgctttatct ttatccggtg ggcagcagca gcgtgtatgt atcgcacgtg
     4141 ttttggcagt taatccggaa atcatcttat tggatgaacc aacaagtgcg cttgatcctg
     4201 tttctactgg gaaaatcgaa agtatgctgt tagaattaag agagcggtat acgatgatca
     4261 tcgtgaccca taatatgtct caagcttcac gtatttcaga taagacggca ttctttttag
     4321 atggtcactt gatcgaatac aacgatacga agaagatatt catgaatcca gaaaaacaag
     4381 agacagaaga ttatatttca ggtcgatttg gataaggagg aaacggaatg ctacgtacac
     4441 aatttgaaga agaattgctg aatctccata atcagttcta cgaaatgggg atgatggtga
     4501 gcagcgccgt gcacaagtca gttcgtgcat acatcaaaca cgataaaatc ttagcccaag
     4561 aagttatcga aaacgatata aatatcaata acatggaaac acgcttggag aagaaaagtt
     4621 ttgaaatgat cgcattgcaa cagccagtta caactgattt gcgtatgatc attacggtca
     4681 tgaaagctag ctcggattta gaacgtatgg ccgatcatgc ggtgtctgtt gctaaatcca
     4741 cgattcgcgt aaaagggcag acgagaatac cggaaatcga aaaagagatc tccgatatgt
     4801 ccgattatgt gaaaaaaatg gtggacaacg tcctagtagc ttatgtcaaa acagacgaaa
     4861 aagacgcacg tacgatcgcc aatatggatc aaagagtcaa tgactatttc aatcgaatct
     4921 ataatgcaac aatcaaaaat atgcaggaaa atcctgagac agtcatcagt ggaacagact
     4981 acttgaatgt tgccagttat ctagaacgga tcggagacta tgtgacaaac atctgtgaat
     5041 ggatcgtcta cttggctaca gggaaaatca ctgagttaaa tacgaaccac aatgagattt
     5101 tctaaactga agctgtgatt atgccgtttg tttaggaaaa gaggttgtga aatcagcggt
     5161 tgtgaaaaag ctgttctgca tcaagtaata agaacagtca aacaaaaaat agctcctcat
     5221 atttttcgtt tggctggact tattaccgca gatgcaagct tttgaacacc gtttattcgg
     5281 gaaagggttt gtgacaaaac atgtcacaaa cccttttcat catttcatag aaaggataaa
     5341 gaatgcaaaa aaaaaattag catcatcact atatttttta tttgtgtgct tacttcatgg
     5401 gttattcagc atcaagctgc tttatatgaa gtacctgtca tcgagatcga aaaagtacga
     5461 ataaaagagg atcatcaatt gattacagga agactgatga acacggatca aaaaggagaa
     5521 tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag aaacagcaaa tataaaagcg
     5581 agacaaatgt atctagtcca aatgcatgga gatgaatggc agctagtagc aaaaaaaaat
     5641 gatggctggc ttttcttctt cacaagtatc ttcattggcg ccatgttgct tattggcgga
     5701 aaacagggtc tatttgcttt aggaagtctc atcatcaata tcgttttact ttacttttgt
     5761 tgctcatctt tactaaaaca gaagggattt ctctattaaa tatatctatc ctgttcgtcg
     5821 ttatagggat gattattagc atattggctt taaatggatg gaatagaagc agtataatca
     5881 aaatttcagc tgcagttacg agtgtttttt tagcattttt tatttgtctt ttgacaatga
     5941 atcattggaa agaccaagga ctgcgctatg aggaactgaa ttatctgaca cgaccgtatc
     6001 gttctgtttt tctttctagc gtactgattg gaacagctgg aggaacactc gataccgtta
     6061 tcacagtcat tgcgacatta gaagaattga ctaagcgaca accagaaata acagcgaaac
     6121 agttatggct ttcaggaagg caagtgggtc gagatgtgat tggctcgatg accaatatcc
     6181 ttttgttcgt ctatatcagt gggtcagttc catcgcttct tttatattta ggaaatcaat
     6241 ggtcgttcaa agaaacgata gaacagcatt tatcattgga atttttacgc gtcattgctg
     6301 gcagcttagg aatagtatta tcgatcccta tctcggtcct cttttttctg actgtaagga
     6361 ggctgaaacg atgaatgtac ttattatact tgttgggatt ttagcaatca gcgtgctact
     6421 tattggcggt acgcaaggta tgtatattct tttaggctta ttcatcaatt tagggatctt
     6481 tttcctgttg ttattcggtt atcatcagaa gtggccgata ctggcattgt cggtcatagg
     6541 atttttattg attgctgtgg ttatcttatt ttttatcaac ggttataatc taaaaatgcg
     6601 tgcagctttt gtgagtatac tgatcttttt attttgtttc ctactattgc ttcctatcac
     6661 tgatttttta gcgatccaag gttttacatc ggtagaacta gaagaattat ctggcttaga
     6721 taagacttta gcgattgatt ttagattact tacgagatcg ttgcttctga ttagtcttag
     6781 tggtgctgtt ttagatgcta gtgtagcaat ttctagtggg acattcgaag tatatcaggc
     6841 aaatccgcaa ttatcttttt accaattgcg ccattctagt ttcgctattg ctaaaaaaat
     6901 attggcgtca acagtaatga cacttctttt tgcattcatg ggaagttctt tagcgttgat
     6961 cctctggttt attgatttag acattccttt tcagcaagtg atcaatgaaa aatcatttgt
     7021 tctagaatat acgatggcta ttcttaccac attgtctgca ctacttgttc ttcctttaac
     7081 ctgcgtgact acagcatatc tctttacaaa aaagaaagaa caactcaaga tggagtaaaa
     7141 gttacaactt aagtcctatg acatttttac tgaatcagtg cataatatgg atgtaaggaa
     7201 aagcttgcaa acaattttgc ttaaggaggc aatttccatg aaagaaagag aacgtatttt
     7261 agatttagtg aaaaaaggaa tcctttcgac tgaagaaggc ttagatttat tagaaagcat
     7321 ggcaactgaa aaagacgaaa agcaaataaa aaaagaagca gacaaggtaa ctgctagtca
     7381 taaagaaaaa gatcaggcaa gtcaattgat cgataaactt gaaaacggcg aagaagaaat
     7441 ttctgagcca gttgatccaa aggaaagaga aagacaagat caagaaaact tagaaaagat
     7501 cttagatgaa ctagcaactg aagcgaataa aacttcggcg cgtctagatg aagtaaatgc
     7561 tcaattcgct gacaataagg cagcacgcaa tgagaaacag gaagcattga tgcagctgaa
     7621 tacaaaagaa gaattaggcg agttgacaga agaagaatta gcacagcgtc agacgttaga
     7681 agcagaaatc aaagaattag aagctagtgg cgatacactg ttagaagaac aattgaaatt
     7741 agaagcagaa ttaaaagata tccgtaagaa tcaatggtct gagaaaaaag aaacgtttac
     7801 tgataagttt gaattgccag atgattggaa agatcaagca acagatacac tgaatcaagt
     7861 aggcgaaaaa atgtcagaag ctggatcaca gttaggcaaa ttcttgaaga aaacattcca
     7921 aactgtttca gaaacagtga atgacaacat ggaatggaaa gatgtcagct tacgtgtccc
     7981 tgggattgcg acaacgaaat ttgagcatga attttactat gaagctcctg cagcaagtat
     8041 ccttgatatc aaggcagcaa acggaaacgt gacgttaaaa acgtgggaca gcaacgatgt
     8101 gaaagttgaa gctaagatca aactttacgg aaaaatgggt gctgaaccat ttgaagcatt
     8161 ctctgaacgg agccagattg aggttaatga agatcatatc tccttccaaa ttccaaataa
     8221 acgtgtgcgt gcagaccttg ttttctactt acctaaacga gtatacgacc acgcagctat
     8281 taaacttcta aatggcaaca tcatgattga aacattggaa gctaaagata tttatacaaa
     8341 atctacgaat ggtaacatca tcgtggacca attaactgca acgatgctgg aagtagaagg
     8401 tgtaaacggc aatatcgata ttcgaaacgg gaacatctta gattctatta tcgaaacagt
     8461 aaatggtaca gtgacatttg gtgcgacagc agaaaacctt tctgtgtctt tggtaaatgg
     8521 tgatgtacgt cttacgatta aagaagataa tttgaaaaaa gtagaagcaa gttcagtgaa
     8581 cggaaatgtc aaagtcgctt taccagatgg catcggtttg gaaggacatg ctaaaacaag
     8641 tcttggaagt atcaacagcc gtctgtccaa ttacgaagta gttcgtgaga aaaaagaacg
     8701 gacaaaccaa atgttgcaat tccgtcgtgt ttcagatggt gaaatcacgc aagtgcaact
     8761 ttcaacgaca acaggaagta tctatttaaa agatacagat aaataaagag gttgtgaaag
     8821 agatgttcag ccttgagcaa taagaagcga ttttgtaaaa tagcttcccc tattttacaa
     8881 aattgtgcct tattgccgat aggctatctc ttgaacaccg tttaccaaga gattatgaac
     8941 ctgccaatta tcgccgagtc acaaggaata atttcagaaa aagctcgtca cgttttatga
     9001 atatggtaat ttgtgaccga gaattttgca gaaagacacc gattaagaag aaggttgtca
     9061 caacgatttt cttcatttaa aatagtagaa cagaggtgag acatgatgaa aaaaaagttg
     9121 acaaaatcca ataaaaatgt tgttttgaca gggactttag caggaatagg agaatacatc
     9181 gggattgatc cgactgttat ccgtgtgctt tatgtattcc tgagcttagt tgcttttgga
     9241 agtccaatca ttttatacat tttattagct ttgattatcc catctgcgcg ttctgcacga
     9301 acaaactatg gacataacaa tgcatattat gaaaaaaaca attatcgtga acaaactcgt
     9361 gaacaaacaa aaaaacgcaa agaagcggaa aaaatcgatg atgacgaatg gagtgacttt
     9421 taatgtcgta ctttcaacgt attttagtta atacgttaac attcatatca ttggctgttc
     9481 tttttcctgc gatggtccat gtgagcagta tttggacggc cgttcttgcc agcttcgtat
     9541 tgtctgtgtt gaatatgttt gtcaaaccga ttttgacgat actgtccctg ccctttacat
     9601 tagtcacatt gggattgttt acgtttgtta tcaatgcagc gattcttaaa atgacctcct
     9661 tttttgtggg agcagaacat tttagttttt cttcattcgg tgcagcgttg ttagtagcta
     9721 tcttgatgtc gcttgtcaat gtaattgtca gcgaacataa cagtagaaaa taaaagaaag
     9781 ctgtgccgaa ctatcggcgc agttttttta tttagaatca ggaatgcgtt ttctattaca
     9841 ttggtaatta aataaaaacg ttttttttaa ttggtagtac ttttggctag acaatgataa
     9901 aatgatagag aacactctaa tagaaatgag gtagcatgaa tgtcagaagt tgtaaaagtt
     9961 agccaattag tgaaaaaatt atctttggaa atcgtgacag gcgacgagaa aagcttagac
    10021 cgtgtcatcg taactgggga catctcacgc ccaggactag agttgactgg ttattttaat
    10081 tattattccc ataatcgttt acagctattt ggcagtaaag aaatcagttt tgcggaacgg
    10141 atgatcccag aagagcgttt gatcatcatg agaagattat gtagtgaaga tatgcccgca
    10201 tttattattt caagaggatt accggctcca gaagaaatga tccaggcagc aaatgaaaat
    10261 gggatagctg tactgcgttc tcccatctca acttctcgtt tattaggaga attatccagt
    10321 tatttagatg gtcgccttgc ccctcgaaca agtgtccacg gtgttttagt ggatgtttac
    10381 ggattaggtg tactgattca aggtgacagt gggataggaa aaagtgaaac agcgttggag
    10441 ttgatcaaac gcggtcatcg attgatcgca gacgatcgag tagatgttta tcagcaagat
    10501 gaattaacgg tgatcggaga accgcctaaa atcctggaac atctaattga aatccgagga
    10561 atcgggatca ttgatgtaat gaatcttttt ggtgcgagtg cggtacgtgg ctctatgcaa
    10621 gtccagttgg ctgtttatct ggaagcatgg gcaaaagata agaaatacga ccgtctgggc
    10681 tctgaagaca cgaccgttga aatcgcggaa gtgggcattc cacaaataaa aatccctgtc
    10741 aaaacaggac gaaacgttgc aatcatcatt gaagtagccg cgatgaattt ccgtgcacgt
    10801 acaatgggct ttgacgcaac gaaaacattt gaagaaagat taagtcgctt gattgaagaa
    10861 aactcaggca acgactagca aggagtgaca atatgttagc acaaatcaat cgagttgctt
    10921 ttgagcttgt agggattcct atctactggt atgcattgat catcgtatca ggtatcatta
    10981 tcgccatgtg gcttagcagc agagaggctg ttcgggtagg attaaaagca gatgatgtga
    11041 cagattttat gttgtgggga ttgcctatct ccattattgg cgctcgctta tattatgttt
    11101 tatttgatct tccccagtat atagctgatc caatccaaat attcaatatc cgttcaggag
    11161 gcttagctat ttatggcgga ctgattgcag gcgcgatcac gctgtatttt tttactaaat
    11221 atcattttat ttctttgtgg acttttttag atatcgctgc accaagtgta ttattagcac
    11281 aggcaatcgg tcgttgggga aatttcatga accacgaagc atatggacca gacacgacgc
    11341 gaagcttctt agaaagtctg catttaccgc aattcatcat tgataatatg tatatcgaag
    11401 ggatctatcg tcaaccaaca tttctttatg aatcactttg gagtttgatt ggatttattt
    11461 tgcttttgtt tttacgaaag aaaccacacc ttttgaaaaa aggcgaagtc cttttatgtt
    11521 atctaatgtg gtatagtttc gggcgtttct ttattgaagg attacgtaca gacagtcttt
    11581 actttttagg tgtgatccgt atttctcaat tattatctgc tgtgttattt gtcggtacat
    11641 tgattctatt tatttggcgc agaaaaaaag gaaatttacc ggattatgac cgctcattag
    11701 gaaaaaatca acaaattatc tgatttttag agcgttaaaa atgaaaaaaa tttcaggagt
    11761 ggtaaaatga attatagagt agcggtttta ggaccaggct cttggggtac tgctttggca
    11821 caagtcctcg ccgaaaacgg acatgacgtt cggatttggg gacatcgtgc ttcacaagta
    11881 gaagaaatca acacccaaca cacgaatcaa cgttatttac cagatcatta tctacccgat
    11941 tccatccaag cattcaccaa tatggaagat gcagtaaaag acgcagatgc ggtgctattc
    12001 gtcattccaa cgaaagcaat acgttctgtt gcaaaagaac tagtgcctaa attgaataca
    12061 aagccagtga tcatccatgc aagtaaagga ttagaacagg gaacacataa aaggatctct
    12121 gacatcttgt tagaggaaat cccagcagaa aaaagaaaag ctgttgttgt tctttctgga
    12181 ccaagccatg cagaagaagt agcgaaacat gacatcacta cgatcacagc tgcaagtaca
    12241 gatgcaaaag cagcaaaata tgtacaagag ctgttcatga ataattattt tcggatctat
    12301 accaatcctg atgtgatcgg tgtagagatg ggggcagctt tgaaaaatat tattgctatt
    12361 ggtgctggcg ctattcacgg cttagggttt ggcgataatg caaaggcagc aatcatgacg
    12421 cgcggattag ctgagatcag ccgtttaggt gtagcaatgg gtgccaaccc gctgaccttt
    12481 atcggattaa gcggagtagg cgatctagtc gttacttgca caagtgttca ttctaggaac
    12541 tggcgcgcag gcaacttgtt aggacaaggg cagcccctag aagaggtgct agccaatatg
    12601 ggaatggtcg ttgaaggtgt gaatactacc caagcagcga tggaactagc ccaaataatg
    12661 gaaatcgaaa tgcccatcac tcaaacaatc tatgaagtgc tgtatgaaca aaaagaaatc
    12721 aaaacagccg caaaagaaat catgttacgt gatgggaaaa aagaaaatga attctcatta
    12781 acaatttttt gaaggagcga atgtatatga aagtcaaaaa agcagttatt ccagcagcag
    12841 gattaggaac aagattttta ccagcaacaa aagctatggc aaaggaaatg ttgccaattg
    12901 ttgataaacc aacaatccag ttcatcgttg aagaagcgtt gaaatcagga atcgaagaca
    12961 tcttgatcgt tacaggaaaa gctaaacgac caatcgaaga tcactttgat tcaaatattg
    13021 aattagaaac aaacctaagt gaaaaaggaa agaccgaatt attgaaatta gtagaagaaa
    13081 cgacagatgt caacttgcat ttcatccgtc aatctcatcc aaagggttta ggacatgccg
    13141 ttcttcaagc aagagccttt attggaaatg aaccgtttgt cgttatgcta ggcgatgata
    13201 tcatggaaga tgaagtacca ttgacacagc aattaatcga tgattacgaa cgtactcatg
    13261 cttctacgat cgccgttatg caagttcctc atgatgaaac ttcaaaatac ggaattatca
    13321 atccaggaga agtattggaa gatgggttat acaatgtcaa aaactttgtt gaaaaaccag
    13381 atccaagcaa agcaccaagt gatttagcta ttattggacg ttacttattg actccagaaa
    13441 tttttgatgt attgtccgag caacaaccag gagccggaaa tgaaatccaa ctaactgacg
    13501 caatcgatac attgaataaa acacaacgcg tatttgcccg taaattcaca ggtaaacgtt
    13561 acgatgtcgg tgacaaattt ggttttatga aaacaagtat tgaatatggt ctaacacatc
    13621 cagaagtagg tgcaccttta cgtgagtata tcctgtcttt aggtaaaaca ttagaagcag
    13681 aaggaagcaa gatcgatcat aatccaaaag gaataacaga aatcaaagaa gaaaagaaaa
    13741 aataaccctt ttttctaacg agcttcagct gctaattgaa taaaaagtag ttgaagctcg
    13801 tttttatttg tttaaaaaac acgaaaatgc tatccttata gaaaagagag acaagaaggg
    13861 agtttgatca tgtcaggagg agaaattgca ggattgattg cagcaatcgc ctttgcgata
    13921 cttgtcgttt ttatcgttcg tgtgttgttg caagtttcta aaactttaga aaaagtagat
    13981 cagactgtag cagaagcaaa tacgacgatc gaaatcgtca caaaagacgt agatcttttg
    14041 tctagacaag tagaaggctt gctggtcaaa agtaatgaac tgctcttgga tatcaatgga
    14101 aaagtcgcaa cgatcgatcc attgttcaca gcagttgctg atttaagtga aagtgtatca
    14161 gaacttaatc attctagtaa aaacatcgcg acgaaagtcg gcggaatcgg taaagcgaca
    14221 gctaaagcga cggttgccgg aaaagtcggt gggacagcga tgaaattttt taaaaacaaa
    14281 aaacatactg aaacgacagg aggaaaataa aaatggctaa aaaaggtgga tttttattag
    14341 gtgcagtagt cggaggaaca gccgcagcaa tcgccgcgtt attgttagca ccaaaatctg
    14401 gtaaagaact tcgggatgaa ttagctgccc aagcggatga tttgatggat atggcttcag
    14461 attacacaga tatggcgaaa gaaaaatcga ctgagttaac aggtattgca aaagaaaaag
    14521 cgcaagatct ttctcaacaa gcaggtgatc tagcaggtag tgtcaaacaa aaagtaagtg
    14581 aatcagcaga tgaagcagga agtgaatctg acgatatctt gttaaatctt aaagaacaat
    14641 cagctgactt atctaatcgt ttcaaaaaaa cggcagaaga tatgaaaaaa cctgcagatg
    14701 aattaggaag tattgctaaa gatacttcag aagatatcat gattgatgta aaagacgctg
    14761 ctggtaaagc aggagatact gtcaatgaag gaatgtcaga agcaaaacca gttgcagatg
    14821 acacaaaaga aatgatgaaa atggcaaaag aagatgcgaa agaagctaaa aaagaaataa
    14881 aagatacaat gacagattca tctacaaatc actaaaatca caaatttata gaataaaaag
    14941 gtgggagcgg actagctctc acctttttta ctcttatttt tctaaaacaa gtaattcttt
    15001 tggtgtagtc gtgaatggca cacagccatc gcttgtcaca tatacacagt cttcgatacg
    15061 tacccctact ttttcaggaa tatagattcc tggttcaaga gagaagcaca tcccttcttc
    15121 gattactaag tcatttcctt ctacaaggga agggaattcg tggacggttg ttccgatccc
    15181 gtgacctaaa cggtgattga aatattcgcc gtagccatag ctgttgatca cgtttcgtgc
    15241 gatttgatca agttcacctg ctgtcacacc tggacggacg gcttcttgtg cggcaagctg
    15301 tgcttcaaga gtgatgttgt atattttttc ttggaagtcg gttggttttt gataagcaat
    15361 tgtacgagta gcatcactgc aatagccatt ccaaacaacg ccaagatcga ataacacaag
    15421 ttcattcggt gaaatcttcg tgtttcctgg tgtaccatga gggcttgcag catttgctcc
    15481 tgcaaggact aatgtgtcga aagacatttg tgaaacacct tgttttttca attggtattc
    15541 gatttctgca atgatcgctt gttcagtcac gccttctttc actgctttaa agccaatctc
    15601 aaatgcaaca tcggcccatt taccagcttc gatcagtttg tctttttcct ctagagtttt
    15661 gatcagttgt agacgttgga tgatggaagt tagatcagtg taagaatccg ttcccgtcaa
    15721 gatatcagag atagcatcga agcgctcgac agtcaaggcg ttcttttcaa tccctacatt
    15781 gccaggctga ccatagcgag cacgaagttc ttttccgatg atctcccaag ggttttcgct
    15841 gtctaaatag ccacggacat catacgccca actactgttt tgagcatctt caacttccaa
    15901 agcaggagtg aaaaggaaag gatcttgttc aagagggata aaaagagcta gtacacgctc
    15961 atgaggatca ctttcgtatc cggaaaagta agcgatgtgg ccagggtcgc tgatataagc
    16021 caattcgact tggtgttgtg ccatccattg acgtagttca ttgattttta cgttattcat
    16081 ttggcaacct actttctaaa ttaattttgt attcaataat tgtaccactg atttattaaa
    16141 agaccaacgt tctaaaaact gaaaaaagta aaaattttca tgaaaacaga gataaaacgg
    16201 ttgcaattct tgagattatt tgatattcta ttttagaaat gtaagacgta ttttcagaaa
    16261 acatataaaa tatgaatagg agaacacaaa tggaaaagca aacaatcact atctatgatg
    16321 tagcgagaga ggcaaatgtt tcgatggcta ctgtttcacg tgtcgtaaat ggcaatccaa
    16381 acgtcaaacc agcaacaaga aaaaaagtct tggaagttat cgatcgcttg gattatcgtc
    16441 caaacgcagt tgcgcgtggc ttggcaagta agaaaacaac aacagttggt gtaatcatcc
    16501 ctgatgtcag caatatgttc tttgcttctt tagcacgtgg tatcgatgat gtggcaacaa
    16561 tgtacaaata caatattatc ttagcaaatt cagatggcaa cgaccaaaaa gaagtcaatg
    16621 tattgaataa cttactggct aagcaagtgg acggcgtgat cttcatgggt caccatatca
    16681 cagacgaaat ccgtggagaa ttttcacgtt caaaaactcc tgttgtatta gctggctcta
    16741 ttgatccaga tgaacaagta ggtagcgtaa acattgatta tgtatccgca acaaaagatg
    16801 cagtaaacaa attggcaaaa agcggcaata aaaaaatcgc ttttgtcagt ggtgcgttga
    16861 tcgatcccat caatggacaa aatcgtctga aaggctataa agaagcatta agcgaaaata
    16921 acctaccata tagcgaagga ttgatctttg aagcacaata caacttcaaa gatggtctat
    16981 ctctagtcga tcggatccat aatagcggtg caacagctgt ctatgtttca gatgatgaat
    17041 tagccatcgg tattttggat ggtttattag accgtggcgt gaaagtaccg gaagaattcg
    17101 aaatcatcac aagtaacaat tcactactga cagaagtagc tcgtcctcgt ttaacaagta
    17161 tcacacaacc attatacgat ttaggggcgg tatctatgcg cctattgacg aaaatgatga
    17221 ataaagaaga agtcgaagaa aaaacgatta tcttgcctta tggaatcgaa gaaaaaggat
    17281 ctacaaaata ataaaaggac aaccattcag aggttgtgtg cctgccgttt aactctgcgt
    17341 tacaaggagc aatttcaaaa aacagcatct catgtttgat gaatattgtg actcgtgatt
    17401 cggagttggc aggagaacac cgtttatttg ggaaaagggg ttatgacaaa attttgttat
    17461 aatccttttt ttgtgtctta aaaaagagag aaagtggttg aaattatatt tatcgtatat
    17521 aaaacaatcc gtgtatagat gctgtatttt gttcaccatc atcaaaaaaa taagggaatg
    17581 tgacaatact attgtcacat tcccttatta ctgtgtattc tattaaatca atcatctttc
    17641 tcatctgatt ctttggttgc ttgattcgct cttgcataag ctgaagcggt gttccagtaa
    17701 tctgtatagt tttcatcggt accgccaacg ccaaacttaa aggcgctttt cttcggtcca
    17761 tttttcgccc atagcgattc aactgttttt cctggtgttt ggatggattt tttattgaca
    17821 gtaacttttc caggtgtttg accagtaaat ccagatactt ttttcttcat cacatcagat
    17881 gataattcga atttctcatc agtgccaaaa atgtcaggtt ttgcttggta agcttgattg
    17941 atcagattgg caagataatt agccgaacgt atccttgctt tgctgtccat tccagtattg
    18001 tcatcgtgcc ctgcccagct actgatggta atagacggag tagagacaat tagccatgaa
    18061 tctcggtatt cattggtaga accagttttt ccgacccaat ctgcttttcc tagatttccg
    18121 tttaagcttg agatggcatc tttgaatggt gtagtgattt ttgcattgat cacactgcgc
    18181 atcatgtcat tcatgatcga tgccgttgct tccgaataga tccggacaga gttgctttcg
    18241 tgtttataaa ttacatttcc tgcgttatct gtgatggaat cgatgatata gccttcttca
    18301 tagacgccgc cgtttgctaa agcctgaaat ccgttggtct gttgaagcgt cgtgacatct
    18361 actgtaccta aaggagcaga ttcaacgccc caattgtcat ttgccgggta gttcatcttt
    18421 gacaagtagt gctcgtaagc atattgtttc gatccaccgg aattcaacac atcttgatat
    18481 aagtgataag ccgggatatt gttggaccat tctagtgact cacggacggt ttggaaggtg
    18541 ttcgatcctt cgttagtagc attgacgatt ttctcgccag cattttctcc ttcttgccaa
    18601 gttgttgcat aatctgatac acgggattct gatccgataa gccccatgtc gattgcaggt
    18661 ccgtaaacaa gaactggttt gattgatgag ccagcttgac gttctgtatc gaaggcatgg
    18721 ttattttgat tctgactgta attccgtccg ccaacgaatc cgtagatgcg gccagttctg
    18781 ttatccatca atacatttcc ggtttctact tgactgccag caccatcatc taacatgtat
    18841 ccataatttt caacgccatt ttgcaatgca gcatagacat ctttatcgat tgtcgaatgg
    18901 attgtatatc ctttattttg gatcgtttgc tgtgcctttt gataataagc gtcactgatc
    18961 gatgaatcag acatatcagc gttgtctttt tcagccaatt gttgtgcaat gattcttgtt
    19021 gcttcattca tcacagtata atacaagaat tctctgtcag tctgttctgc gatttgttgt
    19081 ggtagaaagt cttttgtaag gtcataggct tttgcttctt cgtattgttc tttcgtaatt
    19141 tggccttcac gatacatact gaataaaaca aaatcttttc tggcaaggcc ggcagaaaga
    19201 tcttctttta gcgcacctgt gttcgtgtat ggactatacg tgatcgggct ttggggtaat
    19261 ccagcgatat aagcagcttg cggcagagta acatcttttg catctacacc aaagattcct
    19321 cttgcagctt cctggactcc agcaatgttt tgtcccttat tattacgacc aaatggtgag
    19381 acattcaaat acgtagcaat gatctcatct tttgaaaagt atttttctac ttgtgcagaa
    19441 agaatgatct cgtttgcttt acgtttgaat gttgtttcat ctgtaaggat ctgctgtttg
    19501 actaattgct gtgtcaaagt tgatccacca gaagagccaa taccagtagc ttcagatact
    19561 aatgctcgta aaacagcttt aggaacgtaa ccttggtgtt tatcgaagta ttcatcctct
    19621 gtgctgataa ttgcagtttt caataaaggt gaaattttat cagatgaaat agtcgtgcgc
    19681 atcagatctg tctggatttt tgatatctct gtattatctg cataaacaat ctttgacgtt
    19741 tctgtaatgt ttcctagttc tgtctgcagt gcttttttag tcggcaattg tgtgtcttcg
    19801 acgagatagg caaaatagcc agcgcctatt ccaataccta gagctgcgaa catcaaaaga
    19861 agaacagtaa taaaaacagt caatgattga aaaactctaa aaataatctt aggaacaaac
    19921 catttttctt tttttcttct attctgtttt tttcgagtat ttttgcgtgg caaaagggtc
    19981 acctcaacta atggttttat ttcgctaatt atagcaaata atagaacaaa aaaacagaca
    20041 aggctgtttt tggcaggaat ttctttttta aaggagttaa aacgtgagaa aatgatcttt
    20101 tttataaaag agttcttaag cttttcttta gagaaaattt cagttactat ggagaacagg
    20161 gcaaagaaaa atcaatcatt ttctttgtat atcatcttac caatatatag gaattgttag
    20221 taacaattgc ctttcaagga ggaatccatc catgttatta ggctcacacg tcagcatgag
    20281 cggaaagaaa atgctgttag gagcagcaga agaagcagcc agttatggat cttcgacttt
    20341 tatgatctat acaggcgccc ctcaaaacac tagaagaaaa gccatcgaag agatgaatat
    20401 tgatgccgga aaagcgtaca tggcagaaaa cggtctaaca aatatcgtcg ttcatgcccc
    20461 ttatatcatt aatttgggga atacgatcaa accagaaatg tttccttttg ccgtccaatt
    20521 tctgagagaa gagattgttc gtgcggaagc tttaggcgct actcaaatta cacttcaccc
    20581 aggtgctcat gtaggggcag gaacagcagc cggaattgct agaattattg aaggcttgaa
    20641 tgaagttttg acaaaagaac agacggcaca aatcgctttg gagacgatgg caggtaaagg
    20701 tacggaaatc ggtcgaacat ttgaggaatt agcgcaaatc atcgatggag taaaattgaa
    20761 tgaaaaatta tccgttactt ttgacacctg tcatacgaat gatgccggtt ataatattaa
    20821 agaagacttt tcaggtgtaa tggaagagtt tgataagatc atcgggttag atcggcttaa
    20881 agtactccat atcaatgatt ctaaaaatcc gcaaggttcg cataaagacc ggcacgccaa
    20941 tattggtttt ggaacaatcg gattcgacac gttgaataag atcgtccatg atccaatgtt
    21001 tgacaatatt tcgaaaatct tggaaactcc ctatgtcggc aaagataaaa agaaagctta
    21061 cgcaccatat ggaaaagaaa tcgccatgtt caaagcgcaa acattcgatc cagaagtgtt
    21121 tcctgagtta ttagcagaag aagtgatact ctaagatttt ctatttgaca gcagcaaaaa
    21181 aggaccaggc acattgcctt gtcctttttt gtttaagaaa caaaggagta ctttttgcag
    21241 tctttttcat catgcatctt ctcccccctc tttttaaatt tagacaaaca gggtatactg
    21301 gtagaaaagt aaaaaaacga aaagtttcac aagcaagtgg caaaacgtga ttggaaggac
    21361 gaaaagatgc taaacacttt aaaacccttg atcaaccaac tgactaagaa acatacatac
    21421 tttattcttg gcatagccct attatttaca attattcgtt tgtatttata tagtaaagca
    21481 atctatggaa cagacatgtc ggcaacctat gatgatcagt tgctgattcg ctatgctcaa
    21541 aatattttgg acggtaaatg gctaggggag tatacgacta caacactttc caaaggaatc
    21601 tcctacagct tatttttagt acttgcacat aaactgcatt tttcttatgc agttttattc
    21661 ggcatgttca gtatttttgc cagtatattg gttgcactcg cgtttaaacc gatcgtgaag
    21721 aacaagtatc ttcttttaag tatctatttt tattttttgt tttctccgat tagttttaca
    21781 catgaatatt ccactcgaac gtatcgtaat gccctcgtga ttccggctgt tgagatcatt
    21841 atcgcttgtt tattagctat ttattaccgc aaatacggtt ctagcagaaa attgctgcca
    21901 tggtttttag ggctttctgt cgcttttcct tttttttggt tcattcgcga agattctatc
    21961 tggcttcttc cttttactgt agtagctttg ggtatcagtt tcgtccagat cttgctaggg
    22021 caagactttt ctttcaaaga agtaaagaag ttaaaaaaac gactctttct tctctcgaaa
    22081 cgggaatggc tgaaactttc attgttttta cttcctatag ttggcttttc catcgtctat
    22141 cagtggatcg aatataaaaa tgagacaaca tacggtattg caaccatcaa tgaccgttcg
    22201 agtggagaat ttggacgttt gatgaaacaa ctgatccgaa tagatgatga aacgaacttg
    22261 aataaagaaa acagtgagat atgggtctca catgctgcac taaataaagc attagccgca
    22321 tctcctacat tccatactgt ttctacccgt attgacgaat tatatacgac tcacgcgtgg
    22381 actcaaggtg gaaaagtaaa agaattaccc ggagatatca tcttttgggc attgcgagat
    22441 gtagtaagcg aatctggtta ttaccaaaac aatgcaaaag aaaccaatga tttttggaaa
    22501 aaagtcaacc aagaattaga agaagcctat cacaagggaa ccatcaaaaa gaaaaaagag
    22561 ttctatctga tgggatcagg agatggaaag acgatgagtg atctgccaat cgtattcgat
    22621 ttccttcaat caggatacgc ttatgcatgg cgatataaag atttccatca aggaggaaat
    22681 tatagtttcg gaacgcaaga acaagtcaaa caggctcaag aattattaaa ccttccgcta
    22741 atggagaatt ggatcgatag taatgaaccg agaacaccta acttccaatt aacaaaatca
    22801 gcaaaagtcg cgaatgtcat cataaaaatt taccaagttc tggctattcc cgtgctgatt
    22861 gcttcaatag cagggatatt acttatgata ctaggaaccg tatttgcaaa agctgatcgt
    22921 gcctattata gttcctcttt gattgtggtc accggattaa tactgacaca attggttttt
    22981 cttttcggtg tctcatggtt ttgttcttat gcgccagaaa aaaaggacta ttttttaagt
    23041 gtctacactg gtgcaggcgt gccattaacg caattcatcg tagtaacaag catgctagtt
    23101 ttcagtaaat taaatctgaa aaaagcttta tcttctaaat aaaaagaccc ttggtagcta
    23161 gatttttaac ctaactatca agggtctttg tgtggaaaaa tcagttttta ttcgatttct
    23221 tctaccgttt cctgcaaaag ctgttcatat tttttgtttc ctttatataa atcaccaatc
    23281 gtccatgcca acaggagaag gacaagaaca atgattacat aagcgatgtg atcagccaat
    23341 gccagttgag cggaagtggc ttgctcgccg aagaaacttt cgatctgtcc tggtaatcct
    23401 cgtaagttca agtaagttag actaatcaca gagaaccagc caagaagttt tacgatgaat
    23461 gaatttttaa atcggctacc catctccgct tggctgttgg tcatcatcag taaaggaatc
    23521 atcgaaaatg gcaacgcaaa agccaagaac acctgggatt cattcattaa gttattcaaa
    23581 gcggtatgtt cttcgatcgt tccttttgta cttgtaaaca aaacacaaat caatacagga
    23641 atcacagaaa gcaatcgtgt gaccagtctt cttagccaaa tcgggatacg catatggatg
    23701 aatccttcca tgatgacttg accggtcaac gtaccagtaa tagtagagtt ctggccagaa
    23761 gcaagaagtg ctacagcaaa taaagtggac aagataccag attttgctac agcaatcaaa
    23821 ataccattgc tcatagctga tggatcagat aaagcttcat acagaccaaa aaaggaagga
    23881 tcttggacag aaccggtttt gaagacagcg acacccatga tcaatagcag tgcattgact
    23941 agaaaagcca ttgacaactg gatattagaa tcccaagtag aaaaacgtac agcttgtgct
    24001 acatcttctt cgttttggtg atcgatcttc cgagtttgtg aaactgctga atgaagatat
    24061 aagttatgcg gcatgactgt tgcaccaata atccctaaag ctccagttag cggagtttcg
    24121 ccaccaatga caggtgagtc tgagaatgta tggctgttag ggataaagcc agcaaataaa
    24181 ctgtgccaat ctgggttaga caagccaact tgatagacaa aaacgaataa aatgacgaaa
    24241 attaagcaga caaccagtgc ttcgatctta cgaaacccga cttttgtcag tagtaagaga
    24301 acgaaaacat ctaatacagt aataaagacg gcaatcacca atggaatatc aaaaagtaag
    24361 tagagggcga ttgctgcacc aattacttct gcaatatctg ttgccataat ggctaattca
    24421 gtcaagatcc ataaaatgac acctaatgtc ttacttgtgc gtgctcggat tgcctgtgcc
    24481 aaatccattt ggctcacgat tcctaatttt gctgccatat actgtaaaag catagcaata
    24541 agactagaca tcaaaataac tgacatcaat aaatattgga agttttgtcc tccggtgatc
    24601 gaagtagacc agttacccgg atccatatac ccaactgcta ctaacgcacc aggtccagag
    24661 taagcaagaa gtgttttaaa aaaacttttc ccctttggta catcaactgt tccatttact
    24721 tcagctaaag ataaaccatt tgtatgttca ataagtcgat gcttatgaaa ttgtttattg
    24781 gattgattat tcaagaaaaa accacctttc ctatttatct acgattacat tctacacctc
    24841 aaaaagaaaa aattaaagaa aaagattcgg tatgcctaat ttattttcgt ggcgtgtctt
    24901 gctagatgac gaaagaaatt ttgttctcct ctaaaaaaac cgctattttt tgcatgaaac
    24961 taaagaatac gcctttcttt tttcaataaa ctttagtaaa ataagtcatt gagagcaatt
    25021 cggaaataga aaggagagat ttatatgtta tcaacaggta ttgtcgtact tattgctatt
    25081 attgctttgc taatcggcgc agtaggtggc tttttcctag cgcgtaaata tatgaaagac
    25141 tatttagaaa aaaatcctcc cgttaatgag gacatgttgc gatcaatgat gatgtcaatg
    25201 gggcaaaaac catctgagaa gaaaattcgc cagatgatgc agcaaatgaa gaaccaaggg
    25261 aaaaaatagg ttctgaccca acgacttttt acgggctgat tttttcagcc cgttttttat
    25321 ttcgtaattt gttagactat tcagattatt cagcaaaaaa gacaagcaca gacaatccta
    25381 ggctcttgat aaagacaaag gaggagtaaa atgtcgattt ttagaaaatt aggctggttt
    25441 tttcgtcagg aaaagaaaca ttatttaatt ggagttttct cgctattcgc agtggccttg
    25501 gtacagttag ttcctccaaa agtgattggg atcatcattg atgagattgc cgataaaaat
    25561 ttatcaataa agatcatttt cgggtggatc ttcctattga ttatcgctgc ttttttacaa
    25621 tatctttttc gttatatctg gcggacgaat atttggggaa gtgcagctcg gttggagaaa
    25681 gaacttcggc agcagttatt caaacatttt acagaaatgg acaatctctt ttaccaaaaa
    25741 tatcggactg gggatttgat ggctcatgca acgaatgatt tgaatgcgat ccagaatgta
    25801 gcaggagctg gtatcttgac ctttgcagat tcagtgatta cgggcggaac aacgatcatt
    25861 gcgatgattt tgtttattga ttggcgctta acactgattg cgatgattcc tctgcctttg
    25921 ctggccatca cctcacgagt gctgggatcg aaactccatg atgcttttcg tgattcgcaa
    25981 gaagcttttt ctagtatcaa tgataagaca caagaaagta tcactgggat caaagtcatt
    26041 aaaacatttg gacaagaaaa agaagatcta gcggattttg aagaaaagat tgaagatgca
    26101 attattaaaa acaaacgagt caatttttta gactccttat ttgatccatt cattaccttg
    26161 attatcggtc tgtcttatgt tgcaacaatt attttaggtg gaaagtatgt cttggaaggc
    26221 acgatctcga tcgggcagat ggtctcattt attagttata tcggtatgct tgtttggccg
    26281 atgtttgcga ttggtcgatt gttcaacgta ttggaaagag gaaatgcttc ttatgatcgc
    26341 gtcaatgaac ttttgcatga aaaaacgcat atcgtggaac aactgggtgc agtgcagacc
    26401 ccggcaaaag gcgagctgaa tgtagctatt tcctctttta attatctgaa agaagaatca
    26461 ccagcattag aacaaatcaa gtttgcaatc caagaaggag aaacattagg tattgttggg
    26521 aaaactggtg cgggaaaaac gacgatcatc aaattactga tgcgtgaata cgatgattat
    26581 caagggaaaa tccaatttgg cggacgttcg atcaaagaat attcattgaa tgctttatta
    26641 ggatcaatcg gctatgtgcc tcaggatcac ttcttgtttt cgatgaccat tcgagataat
    26701 atccgttttt ctaatccaaa actttcagaa gaagaagtaa agcatgcggc ttctttagca
    26761 tttatcaaca atgaaatcaa ggaattacca aaaggttatg ataccttagt tggagaacgt
    26821 ggagtctctt tatctggcgg acagaaacaa aggatttcga ttgctcgagc attgatcaaa
    26881 aatcctgaat tattaatgtt agatgatgca ctttctgcag tagatgccaa aacagaagaa
    26941 gctatcttgc ataatttaaa agaaataaga aaagagaaaa caactatcat tgcagctc
//
LOCUS       DACECZ010000026        26258 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.026, whole genome shotgun sequence.
ACCESSION   DACECZ010000026 DACECZ010000000
VERSION     DACECZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.026"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(189..991)
                     /locus_tag="IWO35_001044"
                     /pseudo
     CDS             complement(189..991)
                     /locus_tag="IWO35_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1212..2426
                     /locus_tag="IWO35_001045"
     CDS             1212..2426
                     /locus_tag="IWO35_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0316116.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            2448..4463
                     /locus_tag="IWO35_001046"
     CDS             2448..4463
                     /locus_tag="IWO35_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ0316117.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(4546..5919)
                     /locus_tag="IWO35_001047"
     CDS             complement(4546..5919)
                     /locus_tag="IWO35_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0316118.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(5945..6229)
                     /locus_tag="IWO35_001048"
     CDS             complement(5945..6229)
                     /locus_tag="IWO35_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0316119.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(6231..8246)
                     /locus_tag="IWO35_001049"
     CDS             complement(6231..8246)
                     /locus_tag="IWO35_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0316120.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(8425..8730)
                     /locus_tag="IWO35_001050"
     CDS             complement(8425..8730)
                     /locus_tag="IWO35_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ0316121.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(8819..9850)
                     /locus_tag="IWO35_001051"
     CDS             complement(8819..9850)
                     /locus_tag="IWO35_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ0316122.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10057..10932
                     /locus_tag="IWO35_001052"
     CDS             10057..10932
                     /locus_tag="IWO35_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0316123.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(11084..11926)
                     /locus_tag="IWO35_001053"
     CDS             complement(11084..11926)
                     /locus_tag="IWO35_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0316124.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(12018..13895)
                     /locus_tag="IWO35_001054"
     CDS             complement(12018..13895)
                     /locus_tag="IWO35_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0316125.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(13892..15313)
                     /locus_tag="IWO35_001055"
     CDS             complement(13892..15313)
                     /locus_tag="IWO35_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0316126.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEELRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(15316..18705)
                     /locus_tag="IWO35_001056"
     CDS             complement(15316..18705)
                     /locus_tag="IWO35_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0316127.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            18962..20353
                     /locus_tag="IWO35_001057"
     CDS             18962..20353
                     /locus_tag="IWO35_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0316128.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(20426..21073)
                     /locus_tag="IWO35_001058"
     CDS             complement(20426..21073)
                     /locus_tag="IWO35_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0316129.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(21081..21767)
                     /locus_tag="IWO35_001059"
     CDS             complement(21081..21767)
                     /locus_tag="IWO35_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0316130.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(21773..22846)
                     /locus_tag="IWO35_001060"
     CDS             complement(21773..22846)
                     /locus_tag="IWO35_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0316131.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            23068..23742
                     /locus_tag="IWO35_001061"
     CDS             23068..23742
                     /locus_tag="IWO35_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0316132.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            23747..25087
                     /locus_tag="IWO35_001062"
     CDS             23747..25087
                     /locus_tag="IWO35_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0316133.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(25236..26222)
                     /locus_tag="IWO35_001063"
     CDS             complement(25236..26222)
                     /locus_tag="IWO35_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ0316134.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         7652 a         4948 c         4820 g         8838 t
ORIGIN      
        1 gatggaaacg aattcctgaa tacgcataac agataaatga caaatgttta cacctatatc
       61 ccctgataat acaaccaaaa gtttagaact gaaaataaat ccaaaaaaat tcgctgcaac
      121 gatataataa gacagcaatc gcacctaaaa caattttcct tcatatgctt tagtgaaaga
      181 ttttcttctt aatgtttatt tctccattca ttgggagtac atgttgaata cttcttgaat
      241 aatttgaata aagaagtcgt ttgtttatat ccgatacgat tagcgatttc ttccaacgaa
      301 aaactcgttt cttgaatcag ttccaacgca aagtcgaatt ttttctttag aaccagttct
      361 tgaaaggaag catcaaaata cattttcaat ttattactaa gatagaccgg attgtagcca
      421 aaatgagctg ctgtttcttt taacgtacaa gaagtaaaat gcttatcgat ataatctaat
      481 actctaagca tctcattttc tgcgcatcca tctgctgatg aaaagctttg ttgttctaat
      541 aaatgagtca attcgatcag taatagtgat gtatagagat ttaacgaacg gatcttatga
      601 ctgtatgtat caaaataaat caacagcata ttttcaatca aatgcgtcaa acgacttgtt
      661 tttccgccgc gaaaatgaat gaattgtgta tgttcccctt tttcttttga tgcatttatc
      721 aaaaaagaag aaagtagatt atttgtgctt gccaaatgat tcaatatctc tgtagataag
      781 gtttcttctt ttaataagat gttgattaaa atatcctctt gccctgtgta ttcgattctt
      841 tgaggtgttt actgatatga atatttccct gttgaaatag atcccctcgg tggatttttg
      901 gcggtgtctg aagatggtaa gctaagttta aatcattgat attcattcct tgttcacgct
      961 gcagagtttc gattgattct tcgttacgca aaaaaaatgt ctaattcagc tagttccaat
     1021 tgactccctc ctttttgtaa gcggtttctt tcgcttatta gttcacgctg tactgctatt
     1081 atatgcaaag ctatttttgg tttcataaag actttctatg gttgggacat gaacttcctg
     1141 tgattccgct attgagagaa taaccacttc atactatatt tttgataagc gcttacaaaa
     1201 atgaaaggaa catgcttatg aataaaaaga tgatgaaatt ttctctgctg tcagtctctc
     1261 ttattttgac ttctgctggt gcagtcacgt ctgcaattcc cagtatgtca gctacttatg
     1321 ccacgcgttc tttatctgct gttgaattat taacgactgc tccctctttt atggtcacta
     1381 tttttgtttt gattagtagt tttattgcta ggaaaatcgg acaaaaacaa actgtgatca
     1441 tcggattagt gattgcatcg atttgtggta tcctccccct cttctcacaa agttacagta
     1501 ttgtcttagc ttcaagagct ggactaggta ttggctttgg attgatcaat tctttggcag
     1561 tcagcatgat tgctgccttt tatcacggag atgaacgagc ctctatgatt ggttttcaaa
     1621 gtgcatttca aggatttggt gcagctttaa tgacatttgt tgcaggacag ctagtacagt
     1681 tcggttggca atataccttt ttagtctacg ccatcagctt acctattctt gtcttgtttg
     1741 ttttgtttgt tcctacacct gaaacaactg aacttcaatc gcatggcaca gccccaacaa
     1801 gacaagtaat caaaacgaat cctaaaatga ttagttatgg tatttttctc ttgctggtca
     1861 tcgtggtata taatgctgta tcgattaaat tagccacagt cctgacgact tattctctag
     1921 gaaacgaaac taatgcagcg aatatcttaa caatcatgca attggcaagt atggctgccg
     1981 gagtttgttt tggctggctt cagacaaaag cacatcgcta tatgcttaca ctttctttac
     2041 tactgatggg aatcggcttt gtattgattg ccactgtgat aaacctgtat gtgattgggt
     2101 ttggtgcgat cctaacagga atttcttttt cgttattcat tccttactta ttcaaccaag
     2161 taagtatcca agcgcccaag tacgcggaga gtttcaatac ttcaatctta ttggttggcg
     2221 ctaatttagg tactttcatt gcaccatatg gtttgctctt tctctctttt gcttctcttg
     2281 ggagtaaaac acttgctgtt ttcgtcaatg gcagtatctt gctgttgatt agcgcgctta
     2341 ttagccttct agtagtacac aggacgaata gaaagacagt gataagagta gatgaagaaa
     2401 taaaagaaca attgacaaca aaataaattt gatggaggtc gtgtaaaatg acaaatgaaa
     2461 aacataaaga tagatggctt tggtatcctg gagactttga gatccgccat ggactgctac
     2521 aaaatttcca acgggaagaa cgaggttttg actggccagc gtactggtac atggatgatt
     2581 gccatcggaa cgtcaagttt aagagatatt actttcttga tcaaccttcc atgtttaaag
     2641 tgacgatcca aggagttggg tatgtagaaa ttaatggaca aaaacatcca tgtggaaaat
     2701 ggctgacttg tccagctggt aaagcaaaaa ttcgtatctt tgtaggacat acatcagggc
     2761 taccagcgat gtttatcgag ggagacgaag taaaatcaga tattggctgg acagcaagta
     2821 attttattga agaatatcca gctggctgga gtccgcttta tgtcgatatc gcaaaagatc
     2881 caaatcaaat ctattatcaa aaagaatgcg tccatccagt cactgaaacg gagattggcg
     2941 gaggaatctt attcgatttt gggcgagcag tgaatggctg tgtagaacta atatctctgg
     3001 atgaccatat cgaatctatc actctatgtt atggagagtc cgatgtagaa gctatggata
     3061 tcgattattg ctactataag caaataggaa tgacaagaaa aatgaaagct cgtaaacgcg
     3121 cttttcgcta tttgtttata ccagaggttt ctactggaat ggttcaactt caagtttttc
     3181 atgaattttt atctctttcg aaacaagctg ctttttcaag cgattcttct ttactgaatc
     3241 agatctggtc agtatcagaa gaagccttca agttatgtag tgatttgttt tttatcgacg
     3301 gtatcaaacg agatcgctgg atttggtctg gagatgcgta tcaatcattc atgatcaatc
     3361 aatatctttt ttttgacgag gaaatcaata aacgaacgat attagcatta agaggacaaa
     3421 atgaaatcaa gcagcatatg aatactatcg ttgactattc gatcttgtgg ttgattggta
     3481 tcgaaaacca ctatatgatg acgaaagact atgaattttt acgcatcgtc taccctaaga
     3541 tgcaatcatt aatgagatac ttgatggagc aaacgaatga attagggttt atttatggga
     3601 gagcaaaaga ttggatattt attgattggt ctgaaatgga taaagaagga acgcttgctg
     3661 cagaacaagt cttattactg aaagcctatc attccatgat cacttgcgga gatatcttag
     3721 ctgaaacata tggagagaac gaaaattatc atactcatct ctatcaagaa aaatatcaac
     3781 tattgaaaca aaacctattt gactactttt gggatgaaga acttggcgct tttatcgatt
     3841 gctatgaatc tgggagaaga aatgtgacgc gtcatgcgaa tattttcgct gttttattcg
     3901 attttgctaa caaagaccaa cagcaatcga tcttaaagaa tgtcttattg aatgacacta
     3961 tcacacagat tactactcca tatttcaaat tctatgaaca agatgcttta tgcaaactcg
     4021 gacagacgaa tatcgtctac caaacgattc ttgattattg gggagggatg ttatctcatg
     4081 atgcggtaac tttctgggaa gaatacgacc ctactcaaac aggaaatgaa aagtacagta
     4141 tgtatggaga tccttatgga aaaagcttat gccatgcatg gggagcaagc ccaatctatc
     4201 ttttaggacg ctattttatc ggtttgtatc caacaaagcc aggttatgaa gaatttatga
     4261 tacaacctaa tctagattca tttgaaacac tgaattgtac gctacctatc aaagatggaa
     4321 agatttcgct tgaaaaaaca aaagataaga tcgtgattac ttctgataga acaggcggaa
     4381 cagtgaaaat gaatggtaaa agttatcctc ttctagcaaa acagccactt gtcttaacaa
     4441 gctattcaaa aataaatgca taaaacaaaa aactgcttgc gacatatcag aagactttaa
     4501 atcttcttct gatattgtcc aagcaatttt tattttgaag tttgcttaat tctgttcttt
     4561 agaaacaatg tttgttcttt ttccggcaaa aagaaagcta gataatacga caagtaaaaa
     4621 gaaagcgatg attccaactg tcaaggctat ttgaccgtat tctcctagtt tcccaagtac
     4681 aagtcctggt actagataat cagtatcggc aaatgtagaa gaagcgattt taagatctcc
     4741 taatactgga aggataaata atggcagcca actaatcaat aagctattga aagcagaacc
     4801 taataccgcc cctcttctac cgccagtcga gttgccgaag acaccagaag cagctccgca
     4861 gaagaaatgt cctactaccc caggaatgat aactgtagtt tgcaagccta tcatagcaaa
     4921 catggatagt gttcctacaa agaagctaac aaaaaagcca attaatacag cattaggtgc
     4981 atatggaaaa acgataggaa catctaatgc tggtttagaa tcgggtacga gttttttagc
     5041 aatcccttgg aatgctggaa cgatttctgc caacaccatt cgaacccctt gaaggacgat
     5101 aacaaaccca gctgtgaatg ttccagcttg gatcagtgca aagattacag cgttgtctcc
     5161 attgcttagt tctctttcaa caaatccagg accagctaac aaagcaagtg cgacatatac
     5221 gatcgacatc aagagcatga tcgatacagt agaatctcgc aaaaagctga aacttttagg
     5281 aatctttatg tcctctgtag aacggtcttt gtttccaaaa aactttccga tccatccact
     5341 agaagcataa ccgatatttc cggtatgccc taacgcaact tgattgtttc cagtgatgtt
     5401 cttcataaac ggttgacaaa gagcaggcgt aatagttaat agtgttcctt cgaagacccc
     5461 gcctaataag atcatccaca aatttgcagt aaaccccgcg cttactaaaa tgactgctgt
     5521 tagacaagaa acataaagca ttgcttgacc tgtaagaaaa atgaatttat atcttgtgaa
     5581 tctagcaagt aaaatattca cgatcatccc tactagcatg atcaaagcag taggtgtacc
     5641 gtattgttgc aaagcaatcg ccactactgc ttcattgcta ggaactactc cttgagtatg
     5701 cagagctgtt tggaacatcg aagcaaaagg ggttagtgaa ttactgatta aaccagcacc
     5761 gccgatcagt actaggaatc cgacaaatgt tttgatagtt ccttggatca cttctgaaaa
     5821 tttcttcttc tgaagaacga gaccaagcag tgcaatcaaa gaaacgagga tggagggctg
     5881 actgacgatt gacacaaaca aattcaacaa tttatccatt gatagttcct cccttgaatc
     5941 atttttattc ttttgacaag atcttcgtta ctttttcttt tacttcttcg ctgtcaatga
     6001 tcgattgcaa aggtacgagc ttttcatcag gtatagaagt tagcgtatct ttcaaagaat
     6061 gctcaatgaa gaaataatca gccatatcgc tagatacact tccaacatcc gtatgactta
     6121 cttcaaactc gtccacatct gctccaagat ctgcaagtgc ttgtttgatg ttcatctcta
     6181 ccataaaact tgttcctaat ccactttgac atactgcaaa aattttcata tcattcgtct
     6241 ccttttctta tgatcgctaa tatttcttct tttgttgatg cgtgtaataa atcttgaaga
     6301 ttttgtttat tagaaagtag ctttgttaaa tttgccaaag cccgaagatg tgcctcatta
     6361 tcgatagcag ctaaacagat gaacaaacta atgggatggg ctggattatc aagcagatcc
     6421 acaggttgtt gaagtttcag caaagacatc cctacttcgt ttacgccatc ttctggacga
     6481 gcatgaggta atgcgatgtg atcgccaata tggataaacg caccatactg ctccacgcgc
     6541 tcgatcatag ctttgatata actttcttct attttatttt ctgataatag cggctgtgcg
     6601 gcaaatgtga ttgcttcttg ccagtttttt ggttgatccg ataattggat catttcttct
     6661 gttaataatt cagatagcat aggacgttga tcctccttct tttctaaaag tttgttcatt
     6721 ttacgattta atacttgata gagcttttct tcgtctacgc cttcttttaa ttcgatataa
     6781 ggcagtagcg ccgacaacaa ttcttgggca gaaggcattg aaaatcctgg tatcagccaa
     6841 tctctttgta cttgattgat cagctgattt ttttccaagt cagtcatgat tggatgcaga
     6901 acatagagag actttttctt atcagtaagt gtgagaggta cagtagaaaa aataatatcg
     6961 taagaagttt cgtcaacctc gtgtaagcgg tcagctgaat tagtctctgt aaaaaagatc
     7021 atcgggaata attgctgaag ttcacttttt aagatcaatg aagagctaat gccacttgga
     7081 cagacgatca cggctcttat ttttctattt ctttcttctg cgtccgcttt tctgatctct
     7141 ccgccaaata gtatagtgaa aaaaccaatc tcttcttcag ggatgctctt ccctattcgt
     7201 ctttctaacg gaagtaatgc ttttcttgtc atttcgtaga tggaagcata ttgatgctta
     7261 atttgatcga tcatcacatt aggtaaataa aaagaatagg aaatacgaaa ataggcaggg
     7321 accaagtgat aaaagacatt catcaataat tcacgaaacg aagcaaattc tacggccatc
     7381 aggaattcca tgcgatggat gacttctgaa gaacattcca gcaaaaaatc aagtgcaggc
     7441 tcctggacat ctccttcaac gatcgatgct aagcaaagag agaaaaatat aatctccgaa
     7501 ttttgatgtt cggaatagcc aaggccagtc aatacttgtc gactaatgtc agtgactgca
     7561 agagaagcca acagttcagc atcttttacc agttccatgg atgtttttcc aattcgaatg
     7621 gttgtcaaac tcaaataata agctaattca tccattcgat ttggtgcgaa tcgaagatct
     7681 gatagtgaaa tcgttttatc tagcaaatct ctaactttgg cgtaataatc aatggaaagg
     7741 gttgcggtta aatacgtgag cccaaatctt ccagcaagtg actgcatcaa acgagcagtg
     7801 cgattttttg cttctcttct taatattgct tctgacccta gcagcctaaa accagttttc
     7861 ctagtatacg acagctgaca atcttccact tctaattcat ttcttatctt tttgatatct
     7921 gacaggactg tccctttaga aacctgaaga aaatcttgaa aatgaaaaac agaaacagac
     7981 gttgcagtcg aataaaccat caaataaaga agatctcttc gctcttgttc ggtatacaca
     8041 gcttcaaatc gagtaatatc caaaaatttt tctgcccagt cgattgctcg gaatttcttt
     8101 atacggatac gggtatcatc cacatattcg gcaaaaggag ttaattgttt gatcgcctcg
     8161 cttgtattta cttcgatgat ctctgctgct ttttctagac tgatttctct tggtgttttc
     8221 aggagataac taattttgta gttcacagcg ctgttcacct cctacttata ttctagtatg
     8281 gtagcgcttt taaatcgagg ggtgtttggt acaacttaca gttggatttt ttttacgact
     8341 gtaccgaacg atttctatcg aatggataca gaaaaaaagc aggtgaccaa atgtctcctg
     8401 cttttatgaa ttgatttcgt ccgatcaatg atgatcatgt ttattttttg aggtcagttc
     8461 ttcaaatttt tctttgacat cttcaaccgc tttttcgata tctttttcaa agtgattggg
     8521 ctcattaggc tttgtttcct cttctacaga atttgaacca gataaagctg tttctttatt
     8581 ttctgtttca ccagagtttg tgccagctaa tgattcttct ttgtcttctg tttcaccaga
     8641 attagttcct gctaaagcca cttcttcttc tgttttaggg gccattgaac ctcttaatcg
     8701 tttgtgtttt tcaccgtcat aatttgtcat gttggttcct cctagtagtt ctatactttt
     8761 tcatcgtaca ataagacagt ttatgcgtca aaaaacaaac ccgattctta caaatatgtc
     8821 atatttttcc ttctgattct tgaatgcttt tgattccttc ttccaaaatt ttcatttgta
     8881 agattgtttc ttcatctttg atgacttttt ctgcgccatt aactaatgtc tcgtaaatcc
     8941 cttcgtagac acggctgtaa tcaccaacct cggaaattac tttttcttca tgataactcc
     9001 cttgttcatc tatataggtc aatacaccat aatgttcggg caaatcgata ccaaagtctt
     9061 catgatcagg catgtaaaac attttcagat gttcttcttg tcgatctttt gtttgtttca
     9121 cgaacatccc tttttttccg taaacgacga aacttggacg ttctttgatg cggaaataac
     9181 tggattttat cgaaattttc atatttccat agaaaaaatc cagatcgaag taatcattca
     9241 ttcgtccttt ccctaaaagt tgacgtacat catagctgat atcatctggt tcgccgaaat
     9301 aagaaagaac ttgatctact gtatgacaag catgtccata taaatagctg ttcgcagtag
     9361 aaaattcatg cgtattttct ggtacttccg gtctgaaata atcataatgc atttctactt
     9421 ctaaaatatc ccctaatttt ccgctttcga ttactttttg gactgtcaag aagtcagaat
     9481 cgaaccgtct gttttggtaa cattggataa atagaccttt ttcttttgct aatgcaaata
     9541 actcttctgc ttcctttgac gtttctgcaa aaggtttctc tactagtaca ttcttaccat
     9601 gttcgagtgc catttttcca tattcataat gaaattgact aggtgtagtg atgacaacta
     9661 attggatctc aggatcatcc caaagatcag acagctgatc agtatattcg atgtcttcga
     9721 atgttttcca cactggttca gatggggaaa aaattttttt gatattgatt ttgttcgtaa
     9781 gttttcttga aaaaggcaaa tgatagcgat ttgtactctt tccattgcca agataagcga
     9841 ttgtaagcat gagtggtgcc tccttttatt gatgttttca gtatatccta gaaaagcgca
     9901 gaaagtattt ataatagcta ttctggtaaa aaagttcaga aaaacacttg ttatcattcg
     9961 gtttggtgaa attaatgttc aaagtaagga taaaaatcaa aaagatagta gcggaaccaa
    10021 gtgtttatca gtaagataga atttggaggt cagatcatgc ttattgaaga aaaactaaca
    10081 aaacaagagt tgtttaccac gacagagaaa cggattgtcg attacatccg taggaatatc
    10141 gaagctgcag tctacatgac gattgaagaa ttagcaaaag ctacgtatac ttcccattca
    10201 gctatcattc gtttatgcaa aaaaatggga ttcagcggat ttaaagaatt ccgtttcgag
    10261 ctggggcggg aagttcatca attgattgct cagttcaatc aaacgattga tcctaatttt
    10321 cctttttcaa gtgaagatag cactcagcaa attgcaaaga aaatggcgga tttatcgatc
    10381 cagtcaataa aaaaagcaca attgcagatt gaacatgaag atttagatca aatcggtaaa
    10441 gtattgaaca aggcacagcg aatctttctt tttgcaaaag gtgattctca aatcacggca
    10501 aggaaattcc agaataaaat ggtcaagttg aataagtttt tgatcatggc tgaagaatac
    10561 agtgattctt cttggaatgc agccaatttg actagcgagg attgcgccat ttttattagc
    10621 tacagcggtc gcatccatca ttatgaacga ataatgacct atttgaagca tgtagaggcc
    10681 cctaccttgc tgattaccgg aaatcagcat tcagaaatgg ccaaacaggc tgatatgtgt
    10741 cttgttatat cgcaggagga atatgatttt gctaaagtag cgactttctc ctctcaaatt
    10801 gccttcgatt acgtattaaa tacgttattc tcagtgatat attctcaaaa ttttgaagag
    10861 aatatccgca acttgaaaaa caaacaagaa ttgatgcaaa aaggccttct taaagaatat
    10921 cggaaaactt aagaaaagat cctcatttag taataagaaa gatagtgttt gataggctct
    10981 ctttgatatc atcataaaaa caggctgaag ttttcttttt caaacctcag cctgtttgtt
    11041 tacggaacga ttcgaataga tatattatag aaatgaggaa tagctaactt ctgtttctta
    11101 ttttgaacga ttgacgaaaa agatatataa gaaggaggaa aatcagtata catgcagcta
    11161 acaatccata caggaaaaat cgtttgttct cggatacttt ctgtacttct ttttgaactt
    11221 cttttgcatg ataaggaacc cggtgccctc tgacaagtaa gcgatgactg ttgaccatat
    11281 aaggtgtaca agttaggagt gtcacgaaat cctgcccttt ttcgattctc agatcttttg
    11341 tatcagtcgg ttctaccgtc ttgatctgat cgacttgata agcgagtgtc cgtccattga
    11401 tttcgatata gaaagaatca cctaaagtca gtttcggtaa gtctgtgaac aactttgctt
    11461 gaggcagtcc tcgatggctg gaaataacgg catgtgtatt ttctcccccc gtaggataag
    11521 acgttccttc caataatgag gcaccttttt ctagcagcac agtattcgtt tcatcaaaaa
    11581 ttggcaaatg gacattgatc tcagggatcg tcaaaatacc gatcgtatgt ttctcaaaat
    11641 aggttctatc ctttttcgca ggttcttttt tcttagtaaa gggatctgca cctggatttc
    11701 caccttcttt tgctaattgc tggtttttct ttgtcatgtt ttcttggatt ttagccatga
    11761 cttcttcatt ttctttgacc gcttgttgct gataatgaga gatgatctgc tgatccagat
    11821 aattattcag cgcgtcgcta acaaatggat aagctagtat ccctattcct gagagaagca
    11881 agaaaagcat cagcagatct agaagtttcc ttttctttgg tttcatttgt gttctccttg
    11941 tgatgactgt ttgataataa aagaaaaagg agcatttgcg taatccgctg ctcctttttc
    12001 caattcttgc aaaaagacta aatctgactg tgcttgcgtc tagcaaagta cagtccagca
    12061 atcagtagaa ggactactcc tgcaccgata tacacataga ttcccttacc gcctgttgaa
    12121 ggaagtgtac ctttgtgttt atttggtatt ttttcaggag aaatcaactg tcctgctgtt
    12181 acataagatt gttcatcgat agtaaatgct acacggtttg ttaatggaac atatttttct
    12241 ggcgctttcg tttcttccag atagtacgtg ccatatttta gacctgtcac atcgattaaa
    12301 ccgttacttg tggttgtaaa aaccgttgct gattctttcg ccgataccca gctgacggct
    12361 tttgtggatg ggtcgatcga taaatatttc gctgtgtcac tatcttgatc acgaacgacg
    12421 aattctgctc cagcaagtgt ttggtctgat gtaacgtcac catctacttt aacaaatcgt
    12481 ttgcccccag taacgacatc gactgacggt ggtgtttgat catttgtatg cccgttatcg
    12541 acatttgctt ggttagaaaa tcctttggtt ggatctgctt tttcgttcaa atgcatatag
    12601 taaacgaatt tcaatgtacc gccaggagtt aatgaaggaa tataattcgg atcaactgaa
    12661 accgtgaatc cgtctgtttg ctcagtgaca gaataattta ctgggtcgat ttctttattt
    12721 ccatcatata aggcataagc atacgttcct gaagaatcat tatcaaatgt taaagcagcg
    12781 tcatgagtat cgataagttt gaatgttgtg tacttgtttt gcgttccttc tttatcagcg
    12841 atacctaatg ggatattgac agaaatctca tattgaattt tttcaccgat tgcgacatct
    12901 tttccattca attgaggtgt tgttttatca actttagacg tatcattttt gatggtcttt
    12961 tctactggtg tttggctatt tgccaggatc tcaaaaggcg tgattgtttg cttttcaatc
    13021 atttccgcat tatcaggagc ttttacttct tctaaattat attttccaac ttctaaatga
    13081 ttaacgatca actggccttt ttcaatagat gcctcggcaa agtcattgtt gccgatgtca
    13141 taagaatgac cagtaatgaa atgtttggct ttggtttgat cagttgtcca agtgtacaat
    13201 ccatctgtga cactttggat gtattttttg acgcttggtg ttccttcttc tttagaaata
    13261 ataaattctg ctccatttag tgcttcgttt tcggcagtac cgatttttgt aactttcaac
    13321 gttccatcat taccgactgt atttttaggg tagagatgga tagtatctag ttcttctgtc
    13381 ccgtatttat aagagccatc tgcttgtttg atcatctcat atacagggaa agctaaaacc
    13441 atgtttgcgg cagctgacac tccgtctttt ggttcttctt tgatcgtgta gactgcatct
    13501 ttcccatttt gttttttagg taaagataaa gtgacatttc catcagcatc tgtcgttcct
    13561 gaagcaactg gtgtaccagg agtcaaagac tggactgctt gttttgctgc atccacggac
    13621 gctcctttat ctcgttgcgc ataaaattct tgagtgacgt tataaactga aaatgaaata
    13681 tcggctaatc cttggtattg atcgaattcg ctcatttctt tcccgctgtt ttggattaaa
    13741 ggatcgggta aatcagtcat tttcttttta tgaaggatga cttgagctgc tgtctcctct
    13801 gcaaacactt tttttgcgcc gaagctgttt gtgagtaatg gtaaaataag gaaaaggact
    13861 cctaacataa cgtttatttt tttatgattt ttcatttttt cactcctgtt ctttttttga
    13921 taaacaacca gattgcaaag cttgcgaata ccataccaac aacgcctaaa agtgtatttt
    13981 tcgtttcgtt tgtttttgga agaaatggac ggctattttc ttttttattg actactttca
    14041 atatactgtc cgcctcttta tcctcagaaa gaaaagttga aatggtaaac ggaatttctg
    14101 tttcacttaa aatataacca gaaggtgctt cgatctcttt taatcgataa cttcctgttt
    14161 tcaacccgga aattgaaaaa gcgccatttt tatcggaaat caggtgaagc gcatctgatt
    14221 cgttctttgt ccacttgtat ccgttggctg tttgctggag gtaatttcct gctttgtttt
    14281 taataaggaa tactgcgcca gctaatcgtt tatctggttt atttgtctcg attttgacaa
    14341 attgcttact ccctgtatat acagagactt tctttgtact gacaccaaaa ccaaaatcca
    14401 gtgttccgtt gttgttgatt tccttgttcg cctgtgctgt actgcttagc tgcatctgat
    14461 aagaaatggt cagttttttt ccagcatatt tttccaacga agggatcgaa aataaaacat
    14521 caaatccgtg aggtttcttg tgtatgacaa agccttctgt cagcttttct ccattcgatg
    14581 aaatcgttaa actattaggt aaaaacgtta atgcttcatc cgcactatct gacaattcga
    14641 acttttgata gtcaaggata tttacaggaa ttgtcgtatg caaggaataa gtgatttttt
    14701 caccaatcgt aaaatcattt ctaagctctt ctaatacctt ctcaaaaggt ggatcagggt
    14761 agtcgttttc ttcattttta ggatacaaat ggatagttga aagtttttga ttgttttgtc
    14821 catgtacagg aagaacaact accatattct ctgccttttc tttgacgact tctggtactt
    14881 tggattcaat gaaaagatac gctttatctc ttttttcttg atctttagcg gccagagaaa
    14941 aagaagcgat accatcttcg ttgttaagag ttgtagttgt tgcttctgcg gtaaacatac
    15001 cggaagacgc accgtttttt gcgatctctg cttgtgcttc ttctaccgtt ttgccctttt
    15061 cccgtagatg gtaaaaagaa tctgtgacat cataaacttg gaatgtgaca ccatttaatc
    15121 ctcgatacgt ttgtaataaa gctttttctt gtccgtcatt tggatgattt ttcggcagtt
    15181 gcccgtttgg gaaaagcaat ttgtgtaaag taatctgaac catttctgtt tctgtttctg
    15241 ttgctacctg agtaaaagct ggtttaaata gtaacaagaa gagacacata ctaagccaac
    15301 caagtttttt catatttagc tgccccctct ttcttttttc aaaaaaagat accaaataga
    15361 aaacgcacaa ccaatcatcc ctactagata gattcctaaa cggccaattc ctcccgtttc
    15421 aggtaatggt acttttgcct gattcgtgat gtctaaagaa atctggttgt ttttggctcc
    15481 tggtgacaga acagattcat gatcttgtcc atccacttga attgacccat cttcgtgtat
    15541 aactatagta actggctctt ttagaccttg gtatccttct ggtgtaaaag tttcggtcag
    15601 cgtataagtt ccaggtttta aattttcgaa tagaaaggta tctgtttctt gtccatcttt
    15661 tggcgattct aagtccattt ctggtccctg cagtctgaat tttgctcctt ttaatgtctg
    15721 tccgttgtct tcttttttgt gtaccgttag atcaaacgct tttagtcgat tttgatgagt
    15781 gagcgtccat acattatttg tcatctcggt attttttccc gtgtaaatga tttttccata
    15841 tttatcgatt tggaattcat aatttcccgg cgaagtatca tatccggcag gaccagctgt
    15901 ttcaacgact cgatatttac caggtgtgag tgaatcaaaa ctggcaagac ctttttcatt
    15961 cgttgtttgg ctgtcaattg gctgataagt accatttgct tcttttttct gtagggaaaa
    16021 tgctgctcct ttaagattta tctctttgtc cgtcccttgc atcgcatact tacgtactgc
    16081 tacaggaact tcaacaaaag gattttcaat agtcaactgg atcgtatcat cggaagtagt
    16141 gactgttttt ccatcaatgg ttaccgtacc atcagaagcg atcttgattt cccaagtagt
    16201 ctttttgctt aatccatgcc cttctggagc ttttgtttct gtcagcgtat aggtcatttc
    16261 tttttgcaat ttgacatttt ctggaagaga ataggttccg tcgccatgat ctgttagtaa
    16321 agtatcaata gaatctcctg ttaatttgaa aacagcgcca ataagatcct tttcacctgt
    16381 agaggaattt ttcgttattt tcaagtttaa cggtgtgaat tgtttagtat ttttccatgt
    16441 cattgcatct atttgttgag aatcgtatcc tggtacaggt aattctttga ttgtttggta
    16501 actgaatgct tgaccttgat tattgtattg aggtagtgat aatatctctt gataactttc
    16561 tccgctattt gcagataatt tgtcaatgtc tgcacgttcc cacgtatttg ttgtatcttt
    16621 agctggttta atgattcgaa tatatccgtt tttccaagct gcagcatttg tcgtatgttc
    16681 ccgttgaatc tcaaaagtaa cttgatctgg acgatcagct agattgttgt caaattcttc
    16741 ccataacttt ttgatgtgaa gactgactcc gggagcttta gcagatggta taccgaattc
    16801 agctaattca ttggtatcaa tgtttggctg gaaagttgtc ctgccgttca tttgatacca
    16861 gaaatttgga tggaagtcct cattttctgt ttggattctc acttggtaat ggatttggat
    16921 ttcttggtct tttcctaaat agatctgatt gctcttgatg gtatttcctt ctatggaaat
    16981 agatggagtg accgttgtag gacttgtccc cacactcttg actgaaagtg taccaggctg
    17041 ataaatgaat ggttctgcga ttggatcatt tatttgtcca ttgcttacag ttgctgagat
    17101 ctgtacagct tttttggcta ggtattcaga gatatctgtt gcatgatcag ctgattcata
    17161 gtaaagatcg cctttttcat ctgatgaaac catttgtcgc atacgagact ctacttctgc
    17221 ttttgagaga ccagctgccg gatcgctttg aagttggatg ccaagaccat gtatttcgat
    17281 tcctcgttct ttgagtgcca tcgcttctcc gatggttgcg ataaacgtac tgtcgattct
    17341 tctggataga ttgttttggt caggtgcatc atagattctt gagattagag aagtatttcc
    17401 cggccgatct tgcgtattag aaaactgagt tccgtaataa ttgctgcttg attgtgcgtg
    17461 tacccgctgt actttatagg aaaatgttgg tacaccatcc gtcagcaaaa cgatcacttt
    17521 tttatgtcca tttggaacgg atagcatgct tcctgcatct cttagtgctt tttgtgtaaa
    17581 agtaccacca tttgtccgtg aaggtgtaat ggattttact tgatttttca ctgaatcaaa
    17641 tgaacccatc tgtactgcac cgttactgta gctatatcct tcgcttgagt agccgacata
    17701 tcccatattg attttgtctg tgataccgct atctgctaaa gtatcgacaa aacgatcgac
    17761 accaatcttt acttcaccga tccgattatt gtcgttcata cttcctgacc agtctacgac
    17821 caatacgaga tcaagaggcg tgatatcttt ttgtacattt ccacgagcat tcaaataaac
    17881 atcaaacaat ccgggtgtac ttgtttcctt tgcatacttt cgtatcgcat agtctgcttg
    17941 attaccggtt cctccatatt cgatatacga atgggtccga tcattaggat ctccattcca
    18001 agaatttata ccatcccatt gatttgttcc gtttttattg ccttgatgat caagaacatt
    18061 tgtatttcct gtaggttgcc aactggcttt aggataagtg ccctgatcat ctgttgtata
    18121 ttctgtagga atcgtttgat tagatggcac actcacagct ggcaaggata atgtaggcaa
    18181 tgtaaatggg ctgacagaat tttcgttttc ttttgatgat tcttcttcta tttcgtctgg
    18241 ctttggtact gacacatctg tatctttctt attttctgtc tcatcaataa tgtcatattc
    18301 tttattatcg acgatcagct tgtttgcctg gctgtggtaa agtgctggat cgattggaaa
    18361 actcagatcg atagtttgtt tagtgtttga tgagatttcc aacgtaattt tcccgctatt
    18421 tacctgataa gcatagccat ctccttcttt tctttccaca tcacttaagt aattttcgac
    18481 tagaccaatt tctttttcta tcttttcagc actggtgtta ttcgcattta gcttgatgtt
    18541 cagtgctttt tttccagact gatccgaaat gacttcagct gaaattgttt ctaaaactgt
    18601 tggatcatct gtagtgtctg ctattgcaga taatggcaca aagtttgata aactcaatat
    18661 tatcagcatg aaaataactt tcagtttctt ccctgtggtc gtcatttttc ccctcctttt
    18721 tctttttttc tgttttgacg tgaatagtga aaattattaa aataaacagg cgttacataa
    18781 ttttatttat tttaatatga taattattat acgaagggcg ctaaagggaa tcacaccaat
    18841 tttcatacca attggtttca tttatgaagt tgattccttc gattaatgag cgtttcattg
    18901 aaagaaaagg gttgagggag aaaaacatat gatcaggttg ttggaacgaa actatagaaa
    18961 gatggaaaaa atcattcgaa ttctgtccat ttctgacaag tggatgacta aaaaagaact
    19021 tgccaaacga attggtagtt cagaatctac atttgtaagg tatatcgaag agatcaaaat
    19081 ccgctgggga acggatgtaa cgattcaatc ttcacaaaaa ttggggtatc gtcttgaaca
    19141 tttgaatgta ccagcttatt taaatgttct gatggaaatg tcgcaaagtt ctacaaacag
    19201 tcgtttatta aatgagatta ttgtcaatcc gggtaaaacg attgagtatt attgtgaaaa
    19261 aatttccatc agccggtctt cttttacacg taagttgaaa caatgcaatc aaattttaga
    19321 aagctattta ttaaaagtga tcgttgacca aggttttcag ttaactagcc ttaaaagtga
    19381 gctccagttg cgtatctttg ttaccttttt ctttttaagt tactacggaa gacgcacgtt
    19441 gccatttcca atcgaaaaaa agaagatcaa agaaattatg gtcaagaata attgtcagat
    19501 gaatttgttt caggaagaaa gcccatatga gcaatctttc tttattatgt attttgttgt
    19561 gcatctcatg agagaatccc aaggaactcc atttgaatgg aaagctggag actcttatgt
    19621 acagttgata aatatcgata aaaaagactt cttaatcttg aaaaatctct taggaaaatt
    19681 gaagtacagc agctatataa aagtagtgaa ctattttgtc aatacgattt tttcagaatt
    19741 ttcggagaaa gaaaaaaatg aaatcagtga gcagatacga aaaaatatgt cctactgttc
    19801 gttttcatcc gctgattgca aagaaaaaaa cgaagaatat gttgttcaat tattaacgca
    19861 aatttatttt ttctcccgta ttttcccata cgatacgact catttgacga tccgtgcaac
    19921 ttactttgca cagcagatgc aacaccgcca gatacgtcta tatgctggat ttcgtgcaga
    19981 acttcaattt tattccactc tactgaatca gaatctgatt tattatttat ctagctatat
    20041 cttttggatg cttatcggta atcctgaaat tcaccattat acttctgata aaaaaatact
    20101 gattatcagc gacttaagtc ttcgacatag tcaatatatt gaagaatata tcagtgatat
    20161 tcttgctgtg cataaaattc actctgagac aactgctatt actgaagatc agctgtcgaa
    20221 atacaatctt ctcgagtatg atctgatcgt tacgaatcaa cccattttga atgcccatgt
    20281 tcctcatata ttgattgatg atagtgtgtc attcgcaaat gaagaagaac tcatccgctt
    20341 attcgagtta tgaataatca aaaaacagct atgtataaac caccctgata aaaatttcag
    20401 gacaaataca catagctgtt tctttttatt gtattccttc agcaatccaa cgagatttat
    20461 agcgaaagtc ttttaacaac tcttcttttg tccaattatt tgcaatgaga tccgtcaaag
    20521 tgttcattac gagggtcatg gaaaagtata ggaaactaac tgcttcttcc gacgattgaa
    20581 tggagaaatg attgattctt aataattcca gccatttttc gttcattgga gaatcagccg
    20641 atagttctct acgtttggtt gtctgctgat catttcgctg ggttaacaag tataaccttt
    20701 gccaatagat actttcgtga tctaagtcgc agcaccactc taaatatttt tcgatcccca
    20761 aaaagaaatg ttgtttgttt tcgctaatga attttaagat atcttgatgg actatgtgag
    20821 aactgctttt gatcgtatac aaataagcat cttccaaatc ttgaaaatat ttataaaaag
    20881 caccgcgcga catccccatc ttgtcaacga tttctgagac tttgacttgg ctgatgtgct
    20941 gtgagcaaaa tatctccagt aataggtcat cgattctctg tttctttgcg ttcgataaat
    21001 tgaaaaaagt ttcttttggc atccacatct ctcctttatc tctgattata cgtggatagc
    21061 ccttcattgt catgaaggat tcattccttc tttttctctt cttctgtcaa gtacccatct
    21121 tccattcgat aaacgcgatc actccattga atcatacgga catcatgagt aaccataata
    21181 gtcgcttttt gtttctcatg agcttcttta actagtaatt tgacgacttc atatgcatga
    21241 tctgtatcca aactagctgt tggttcgtct gctaagatca agcttggctc attatataaa
    21301 gcgcgagcaa ttgccactct ctgtctttct ccaccagaaa ggtctttagg ataactattt
    21361 ttaagtgact ggatatctaa agattgcagc aaggaatcga tttttgttga atcttctttt
    21421 tttgaagtaa cttggtcaac tagttcaaat tgttccttta ccgttaaaaa aggaatcaaa
    21481 ttagagcttt ggagaataaa tcctatttct ttaaagcgta aatctgctcg ttttttttct
    21541 ggaagctcag ttagtgagtg accgttgatc actatttctc cggaagtcgg tgtttgcaat
    21601 cctcctgaaa tggtaaggaa agtactttta cctgaaccag aaggaccaat gatgctgacg
    21661 aattctcctt cctttacata aaaatcaatt gctttcaaag ctgagacctt tgtatgacca
    21721 gaaccaaatg ttttggtaac ttttttcatc gttaatattt ttttcatttt tattaacctc
    21781 ctattgctgt aattgggtct actttggtta tcgtacgtat cgaaaacaat ccgcctagca
    21841 ttgcgacaat gatcaaaatc ccactatata gcaaccattg tgaccaaaaa acagcaaaag
    21901 gcatcgcgct tggtaaaatc agactagtca aataagcaaa caaaagagca agcagaacgc
    21961 ctaccacccc aacaataaag gactgtgcta ctaaagattt agcgatgaag aaatttttga
    22021 tcccttgtgc tttcattact ccaaaaatag cagtcttctg aagtgtcatc acgtacatga
    22081 aaataccgac cacggcagca accactaaga acaaaaagta tatcatcgca tttaaggtca
    22141 tattttgggc agaatagcca ggaatacttt caatgaatgt cccaatagtt aataactgta
    22201 gacccttcga tgctttttct ttcgaaattt tagctgaatc ctttattacg atcccattga
    22261 tcggctgttc ttcttcagaa gcaaacggct gttgtccata cttcaaagca gtccacgtat
    22321 ctagggacgc atagatgacg ggagaaactg tgtaataagt ttcaggaaag ataccgacaa
    22381 tcgtcaaagg atcgtcgtaa ctgccaattt tgatcgtatc tccaatttta tagcctgaat
    22441 cagccagatt ttgagagatg atgatttcat tctttgtttt aaattcatgc cctttcgtga
    22501 gttttggtaa taaaaatgct tctttcgttg tgccgaaaac tgtaacattt tctttgtttt
    22561 ttcctttgat tgctccgctg tacaatccga ttggtgcttt ctctcctcct tcaatgtatt
    22621 tcagatctcc tcttgttaat tgtgaagctg caaatacttg atttgcatcc tccgacaaaa
    22681 caatctcttg tgcgtcccaa tcatcgattg cttttttaaa ctcttctgaa agtccattcg
    22741 ctagtcctga caacatgaat acgacatacg caatcaagaa cataataccg acgatcaagc
    22801 catatcgtaa tttagaatag cgaatttctt tgatagctag aaacatttgt ttttcctccc
    22861 tatgatagat tgatagtgac aaaatgtcac ttgttgaata gtgtaactct aaagtgacaa
    22921 tttgtcactt gtgttgtttt tttcttatca ataaattgtc taagacatct ttcgataaaa
    22981 ccttaacatg agtcataaaa aatttattta agccttattt atgctttcca tgataaacta
    23041 aagtgaccaa atcaatgagg tgggaaaatg attaaactat tagtagtaga agacgataaa
    23101 gtattatcca ataatataaa agaaatactc gctgcaatcg gcgagataac acaagtttat
    23161 gatggtagtg aaggactgtt cgagttggaa agtggagttt atgatcttgc aatcttggat
    23221 ttgatgatgc cggaaatgaa tggatatgaa gtgttagcaa aaatacggaa agaaaaaata
    23281 cagactccag tattgatttt gactgcaaaa gatagtttgg aagataagat tgaaggattt
    23341 gaacgaggtg cagatgatta tctaacaaaa cctttctatc gagaagaact gatcatgcgc
    23401 gtgaaagcat tattgaaacg ctcgctaggt ctctttgatg aaaatgttct gaagtataag
    23461 gatatcagtt gtgatttaaa aagcaatgaa gtcttgtata aagaacaagt tttgcccata
    23521 caaggaaaag agtttgaatt gctcgtttat tttttacaaa ataagggaat cattttgaca
    23581 aaagagcaga tatttgaccg gatctgggga tttgattctg atacgacgat tacagttatt
    23641 gaagtttata tgagcaactt acgaaaacat ctgcgaagta ctggattgga taaagaaatc
    23701 aaaaccttac gtaatgtcgg ttatatactg caaagggtgt gatgcaatga atggagatat
    23761 tacacgaaag caacgtagtc gcttttttgc gacaaatgta gcagcatttg cttttatctt
    23821 ttttcttttg ggattcatta ctttacagat attaaaccaa tctgcataca gagaaacaga
    23881 tctgtcatta gagaaaataa attttgattc tcgaacagta caattagaga tacaaagata
    23941 tcagcaaggc gatccctttt taaacccgac tcaaacggaa caaaacagaa caaacatgga
    24001 acagccatta atggaaccat caaatgaccg ttttaacact caagtgatct tgtggtctaa
    24061 aaatggtgag atattgaata aagcagctat tggtgggcgt ttcaaccaaa tcaaagaatt
    24121 aaaaatggat acgaaaaatc tgaataccat ccaaaatatt gaattaaatg aagacagtgg
    24181 ctcatctgcc ctctctttcc gttcgattac gaaaaaagcg gagacgaatc aagatggcgt
    24241 tgcctacatc caaattttag aaaatacaaa tcaaatcaag aattcattag cgacttttcg
    24301 cacgattatg attttgtgta tgattgtttt ttggattctc tctattggta tcagttatta
    24361 cttgtctagt ctaagcatgc gccctattat tctttcttgg agaagacaaa aagaatttgt
    24421 tgagaatgct tctcatgaat tgcgcacacc gttaacgatt attcaaaata gtttggaaca
    24481 tctttttacc aagcctaatc atactattat tgaagaatcc gaaagcatcg ctcaagcatt
    24541 gtcagagacg aggcgcctca ctggattgac ttcggatttg ctgacaatcg ctcggaatga
    24601 ttctgatcag cagctgcttt caaagcaaat gatcaataca caagaatata tcgaacaact
    24661 tgttaaacca tttcaagaga tggccatcat agatggaaaa gagtttattc tccataacga
    24721 tgcaacatta gaagtaccaa ttgatgaaaa aaaaatccat caagttttgg ttattttgct
    24781 ggataatgca ttaaaatata cgaatccaga agataagatc attttggatt catctgttaa
    24841 ccataaagaa tggctgattc acgtaaagaa tactggccct tctatcagtg aagaagatca
    24901 agcaaggatt tttgatcgat tttatcggga agatcaatct cgggcgaaag agactggcgg
    24961 atacgggctt ggcttggcaa ttgcaaaaca ggtaatcgaa cagcatcatg gacagatttc
    25021 tgtatccaat tatcttccac aaggagtcga ttttaaagtc caattgccgt tgaagcctaa
    25081 aaaataatgc ttatccctaa aaaagggctg tgacaaaaat cttgtcacag cccttttttg
    25141 ttgcgaataa acggtgttcc agaagtagct tcttcggaaa taagccgaaa tttctttaag
    25201 ctgaccactt ctgtcacaac ctctttagct tacgcttacg attttttatt gcggtcttca
    25261 atgtgtacat caccgcttgt gtcaatatcc aattcttctc ggctaacatc ttctgtcact
    25321 ttttcaacat cttcatgttc ttctttatga atcccaactt cttcacgaat aactgtatgt
    25381 ttgcttacat ctacttgctc ttctttgatt gggatagtaa tcgtgtcatc ttccaatttt
    25441 tcgtctgttc cctgtgaaga ttgatctgtt actggtttgc gttcgatgac gatttcttca
    25501 cgtttgaccg gtacttctac cgtttcagta tcgttgacca catgtttgtg gatgtcgact
    25561 tctccagttg tcacatcatg tgtatttaca tctaattttt cttcttttag ttgaattgta
    25621 tcatcatcta gatttggttt gcgatcgatg tcttcagtct tgcttgtacc cattgtatca
    25681 gtctcgccag gtacgtccat cggatcagtt cctggtgtca tattcaatgg atccgttgta
    25741 ttttgaaagc ctgtcgtgcc acctacgtcc attggatctg ttccagtgcc agtttctaat
    25801 ccagtcccca ttgtgcttgc tgggctaaca gtatcttgtg tatccattgt atcttctgtt
    25861 gtttctggct gacgatagcc ttttactgtg atgacaaatt ttccatcaga aatatctcct
    25921 ctatattgat ataaaggatc attttctggt gtagccgtct cagaatcata ggtatcaaaa
    25981 gaaaaagtat cttttatttt ttcccataaa gaacggtctt cttcaccatc catccgattt
    26041 tcatctgttt caacatctat accagacaat ccgtcaaatc ctagtctaga tgctgcttca
    26101 tcagtagtat aaataacgat atcatctcgt tcataccctt cgttacgcaa ccgttcaatt
    26161 actgataatg cttcttccct cgtactatag cttccgatga ccgttaaatt tttctcttcc
    26221 atgtaaattc ctcctagcaa atagatttat gattggta
//
LOCUS       DACECZ010000027        25975 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.027, whole genome shotgun sequence.
ACCESSION   DACECZ010000027 DACECZ010000000
VERSION     DACECZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.027"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(231..653)
                     /locus_tag="IWO35_001064"
     CDS             complement(231..653)
                     /locus_tag="IWO35_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0316135.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            complement(643..2817)
                     /locus_tag="IWO35_001065"
     CDS             complement(643..2817)
                     /locus_tag="IWO35_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HAQ0316136.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2833..5175)
                     /locus_tag="IWO35_001066"
     CDS             complement(2833..5175)
                     /locus_tag="IWO35_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316137.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(5197..7740)
                     /locus_tag="IWO35_001067"
     CDS             complement(5197..7740)
                     /locus_tag="IWO35_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HAQ0316138.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7750..8148)
                     /locus_tag="IWO35_001068"
     CDS             complement(7750..8148)
                     /locus_tag="IWO35_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0316139.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(8229..8513)
                     /locus_tag="IWO35_001069"
     CDS             complement(8229..8513)
                     /locus_tag="IWO35_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316140.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8537..9535)
                     /locus_tag="IWO35_001070"
     CDS             complement(8537..9535)
                     /locus_tag="IWO35_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0316141.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
     gene            complement(9528..9875)
                     /locus_tag="IWO35_001071"
     CDS             complement(9528..9875)
                     /locus_tag="IWO35_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316142.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFLEGASVVVHEVVIVDKI
                     PKLLLERYGISHE"
     gene            complement(9891..10541)
                     /locus_tag="IWO35_001072"
     CDS             complement(9891..10541)
                     /locus_tag="IWO35_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316143.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(10552..11724)
                     /locus_tag="IWO35_001073"
     CDS             complement(10552..11724)
                     /locus_tag="IWO35_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ0316144.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(11743..12615)
                     /locus_tag="IWO35_001074"
     CDS             complement(11743..12615)
                     /locus_tag="IWO35_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0316145.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(12637..13557)
                     /locus_tag="IWO35_001075"
     CDS             complement(12637..13557)
                     /locus_tag="IWO35_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316146.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(13557..13826)
                     /locus_tag="IWO35_001076"
     CDS             complement(13557..13826)
                     /locus_tag="IWO35_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316147.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(13823..14203)
                     /locus_tag="IWO35_001077"
     CDS             complement(13823..14203)
                     /locus_tag="IWO35_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAQ0316148.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(14670..16133)
                     /locus_tag="IWO35_001078"
     CDS             complement(14670..16133)
                     /locus_tag="IWO35_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0316149.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(16197..19214)
                     /locus_tag="IWO35_001079"
     CDS             complement(16197..19214)
                     /locus_tag="IWO35_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0316150.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(19365..20204)
                     /locus_tag="IWO35_001080"
     CDS             complement(19365..20204)
                     /locus_tag="IWO35_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0316151.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            20349..20594
                     /locus_tag="IWO35_001081"
     CDS             20349..20594
                     /locus_tag="IWO35_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316152.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            20657..21079
                     /locus_tag="IWO35_001082"
     CDS             20657..21079
                     /locus_tag="IWO35_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316153.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(21173..21490)
                     /locus_tag="IWO35_001083"
     CDS             complement(21173..21490)
                     /locus_tag="IWO35_001083"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316154.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(21517..21723)
                     /locus_tag="IWO35_001084"
     CDS             complement(21517..21723)
                     /locus_tag="IWO35_001084"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316155.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(21704..21886)
                     /locus_tag="IWO35_001085"
     CDS             complement(21704..21886)
                     /locus_tag="IWO35_001085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316156.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(21900..22250)
                     /locus_tag="IWO35_001086"
     CDS             complement(21900..22250)
                     /locus_tag="IWO35_001086"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316157.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(22276..22578)
                     /locus_tag="IWO35_001087"
     CDS             complement(22276..22578)
                     /locus_tag="IWO35_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316158.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(22581..22814)
                     /locus_tag="IWO35_001088"
     CDS             complement(22581..22814)
                     /locus_tag="IWO35_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316159.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(23800..24122)
                     /locus_tag="IWO35_001089"
                     /pseudo
     CDS             complement(23800..24122)
                     /locus_tag="IWO35_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24245..25360)
                     /locus_tag="IWO35_001090"
     CDS             complement(24245..25360)
                     /locus_tag="IWO35_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316160.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(25366..25764)
                     /locus_tag="IWO35_001091"
     CDS             complement(25366..25764)
                     /locus_tag="IWO35_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316161.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         7089 a         5457 c         4057 g         9372 t
ORIGIN      
        1 aaacggattt caaacaaaat acgtttattt tttctattga ttttagctca aaatcgattc
       61 aattcgagtg aaatttgctt caattttcaa ttggtttttg ttcaaatttt gaaacatttt
      121 tgctcaaaaa atgagtgaat ttgtttcaaa agaaaaacaa atacgatcga aaaaaatttg
      181 aactcataac aattttttgt ttttttcaac gtattctctt tcaattttca ctaaaaatta
      241 ggaagaaatt gattcaatgt cttttcaaaa aagaatgttt ttctctcaat ttttttgacg
      301 tttctatcat gaatcacgag aaaacttggg acacttttaa catggtaacg ttgtaaaaaa
      361 gtttgaagtt cttcttgtct tggttctttt cttgtttcaa ttcgatatac attcctagag
      421 aattttttgt cgtatctttc caagaaagat tcaacttctt ggcatttttt acaatcattt
      481 cttgctagat acacaaaaaa tgattgattc gttttttgaa gttctagtac ttcttctaaa
      541 ttactattga ttagttttcc atttgattct tcgttagttt ttatcagttt aaaacaacca
      601 aataagagga aactaacaat aagtatccta acaataactt tcttatttcg catattgttt
      661 ctccaaattt ggtttccgaa tatagacgac tcgcggtggt tcctggacgt cttcagctgt
      721 tcgatacacc acaaattcgc ctgttctcaa tgctcgaatg cgattcggat tgaatttaaa
      781 gcggtctacg tctcgtttcg tgcctctttc tcctgtccaa tcaacctttc ccatatcaga
      841 ataacctgct tcttgtgtcg tcatttccgt taattcaatg tcttcttctg tccccattaa
      901 atctgcccaa taatcaattt ctgtcgggtc atttgtcttc ccaataaaga acgtattcac
      961 attcccaatc aactgttttc gaaggacagg atcaattcta tcaatgtccg caagcgtttg
     1021 aggggcatac gccgcatgca ccccagcgcc tcgtccctta ttgactaagt ctataaggtt
     1081 atcatttaga taagaagctg gttcgtcaaa tattactagg actttcgagc gattggctgt
     1141 ttctcctgct tgtttataca cttctgacgc aaagaaatta atatctccca caagcatttg
     1201 agccaatgtc cgaataaact cactataaat caatccgttt aacgagatat aaacaatctt
     1261 tccttcttta acaacttctt gaatatctaa ttctcttttt cctccagaag tatcaaagag
     1321 ttcaccaagc tcactatcca ataacatatt gatattggtc tgtaaattct cagaagtcgc
     1381 atacaaataa aacgggttct tcttatgttc gtaacgaaca aacaaccgtt taaacaacgt
     1441 ttgctcgatt tctgtcatga gagaaatgtt ttcttttaat aatagataat acgtttctaa
     1501 gtcaacggtt ttacgatcat gttcaacgat tcgctcttca cttaactgtt ccacatcggc
     1561 aaaatcacgt tctgttttgt ccatcgcttc attctttttc gtttttttct tcgtggtttt
     1621 cttcttttct acgaccactt tttctttaaa taaatctgga tttttcaact gaatacgatc
     1681 cgcaaacaaa tgtaagacgt ttcgtggcaa taaataatgt tgtaaccaaa ctagatttct
     1741 ttcaatttta tggctctttt ggaactcgtc taaaagctga atcgttacct gtaataacgc
     1801 tttggctgct gtcgaataat acacgctttc tgtttctgct agattggtta atttatctcg
     1861 aatggcaata tcattgccat tttttacagg attataacgc aaatcgccac cgtccacaaa
     1921 gacttcaatc gggcgattgt atgcctctgc cacttctttt aacgcttgta aagtctctcc
     1981 tgctcctttc ccatcgacta agataacggg gatacgaaac ttacacgcat gttctgctag
     2041 taattggatc accgtcgttt ttcctgaacc tgtagtcgct ggaataatca catgttggtt
     2101 caattcacta aagttcatca cataagggag cccatactca tctgttccta atagaatcac
     2161 tttggctaat gaagccagtt gtttattccg caaagcaacg tcttttatga caattgctac
     2221 ctggtacgct tgctgatact ctgctgtata tttttgggac tcctgaaatt tattcttccg
     2281 acgttccttg tattctttac tatttagcaa agctgtttgc cgttctttct ttgaaacaac
     2341 ttttcttttt tcaataaaag acgcatacgc aatcaaacaa cttcctgcac ctgtcccacc
     2401 taaaaggagg gcaaaaatcg tttgtccatt aaactgcaac gcatgagaaa acaagtttaa
     2461 tggaaataat aagtaacacg acaaataccc tctaggaaga acaaaaataa aacaaaatag
     2521 atttaatagc gtaaaaccta acgcaaaaat aaaaaaaaga gagcttcttc gttttaagaa
     2581 gctctcatac tcaaagtcat aaatatgtgc acgtgtctct tttttatcta tcataaagac
     2641 aatcgctaaa ggaatactgc ctacaataaa aaatggatat agacaaacac ctaataaagt
     2701 gacgataaaa taaagttgtt gaaaccgact ttttggcgtt tctttttctt gttttctttt
     2761 gcgatcgaca gaaaacattt gctcaaaatg tgtcggtcgt tttggtgcat tcatcatact
     2821 atcgatttcc tcctatcgtt ttcgttgttt catttctctt cgataaatcg gttgtttttc
     2881 tcgttttcgt ttcatttttg cttgagttgc tcgaaactca ctccgtgtct tcggtgcttg
     2941 ttttggtgat tttgtttgtt tcatggttga gtatgcttta gatacggttg aaggcgcttt
     3001 ggtttgtgct gttactcgat tgactgctga catatgttcc cgaaccccct ctggacgtcg
     3061 aacagatttc ggtgtggtag aacctgttgc tcgtggtaca ttcccttttc ttagtggttt
     3121 ttgtaccgca tgtttttgtc ctgcaactgt ccgggttttc atcgaacttg tttttgttac
     3181 gggagtaagt gcctccttcc gacgacgact tgtttgtccc tgacgacgtt ttcgatcgtt
     3241 caattctgtt ctaggcgtat gcttctggcc ataagacttg tgtttttgct ttggattcgc
     3301 taatgacacc accattgcag aaccctgatt gcgatacgga ctacgagacg caccttttct
     3361 acttctttct cttagactag gattgccttg caattctcgt tgtaataaat cggctgtttt
     3421 tcttgcctgt ttctccattg ctgttcgttg catacgttct tgactttgct cttctgcttg
     3481 tttcgcttga tcatgcaagt ctcttgcttt ttgtgccata cgttccatac gatttggtgc
     3541 tttatgacga ggttgttgtg cggattcacg ccgaaaagca cttcgtgtgg cttgtttcgc
     3601 cgtttgtggc atacgaacgt ctgagccacc cccacgtttt cgttgacgtt cttgtctctt
     3661 ccgtgcgttt tctttttctt tttgtttctg tgcttttctc gcttctttgc gttctttcaa
     3721 ccgttgtttg gcttcccttc tcaattttgc ttctgcccca aatggatttc ccgcaaaagc
     3781 taacgctcct cgaacaccca ttcttccagt aaatagactg cctaaaaagc ctcggaacaa
     3841 ataaattccc aacggcgcaa acaaataaac aatgatcaag agaatttttt cccatggatc
     3901 gtcttgtgta ctacttaatt ggaacccgta actaagaaaa gaagaaaaaa ggatacatgc
     3961 aaaacctgtc attcctttta aaaacgtcaa aataccaaaa actttggcat agttaccaaa
     4021 cacattcatt gtgacaagaa acaatcctaa aaaaagtaag aacggcaaca tcggtactaa
     4081 caatagttgc atagttccaa taatcaatcg aaacatactt aagacaaaat aaacaacggt
     4141 ttgaatcaga ttcacgacaa aataaaacaa ggcaaatgct gtatttttcc aactattgta
     4201 atattgtatg ttcttgtttt tcagtgtatc tgcttcatac tctgtgactt tgtcatgaat
     4261 tttctcccct atttttgtac ctaaatcatt gtccaataaa agattgattc gatcatattc
     4321 ttttccagca tatttgactt tcttctcacg aattgtgtct acattggttg tgccataatt
     4381 taacaacaga tatggttcat aaacattact ataaaagacc cgagccgcca tcaatttgcc
     4441 tgccgtttcc acttttttat ccgctgttac ctcttctgtt tcttttgttt cataatttgt
     4501 ttcttcttca ggtgttaaca ctggattgac cttcacgaac gccgtttcca ctgtagaatc
     4561 taaagaaaaa agaacatcaa aaagattctt atttgtccca gaatctttta atacgcttaa
     4621 cccagtaaaa ataaggatcg tcataagaaa gacacggagt gcttgtttca ttcgagcctc
     4681 tccagcatat ttgattgcca tcataattgc cgaaaaactg attccaatag ttcctgcaat
     4741 acttaacata ttgtccgcaa ttttttgtgt taagttttga atgggttctc gaatggcgga
     4801 aataatatct aattcaaaga gaaattcaac cattgaagca ttgaattctc ctaacatttt
     4861 tacgccactc caaacaaaat ttttcatcac tcctgcaaca ttgtacgata gctttttttt
     4921 aaggttcatt gactcatagt cattggtcat caattcaaac gaattctttt tgtaattttt
     4981 atacaattga tccacatcag tcgttacttc ttttacttct actttatcgt tggcattctc
     5041 tccttcagaa cgatcggttt cagtgacacc cgcaccatca ttcatttgat cggcagaaac
     5101 agtagcccct tgtagacaga aaaaaagacc ccctaataaa aggagcgtcc accatttcag
     5161 agattttttt cgcaaaaaaa ccacctcttt tttgttttat cgttgttttt cattttctaa
     5221 cattgcttga attttttcat cttgattttc agtagaagaa acagccaata accaactatc
     5281 aaacaacaca tcaatggcaa tcggttgatt tcttcccaaa tgatcctgga ataaacaggt
     5341 tcctgtttgt aattgttcca tcatttctaa attggctgaa ttcacttcca tatcaaagaa
     5401 ggacaatagt ttctctcgtg cttctttttg tttcggacga aacgcaaatt tgtaagaaat
     5461 taactctttt ttatcttctt gatcgtaatc tttaaacgct tgcacaacaa ggtaaatatc
     5521 cgtattattc gcacgtcctt ttcgcaagct atcatcaatc aaatagcttc cttgtgcggt
     5581 atcttcatac ccttttgcct cgtcataaat aatcgcagcg tcttcttctt tgtttgtaga
     5641 aaagatatac gtaaacttca taatgacttc cataattgcc attcctgcaa gttgttcgct
     5701 atttaatcga cccgaatcaa tctctttttg tgtcggaatg cgtattcctt gtgtccctaa
     5761 cacgttgatt tgtgaatcaa aacgtatcgg cgtactgtgc tcattcccaa tgagaatctt
     5821 tcctaaacca acgctatgcc ctaaaatcaa tttagctagt tctttgtcac tttttcgaat
     5881 ttcttcgata acttgactta acgaatgctt acctggcatg gtgtaaacta catcgatggc
     5941 gtcttggatc accgttttct tgtggctcgc tgtttctcga ttggtatcta cttctcctaa
     6001 cattcctaac acatttttag cggtttgaat tgcctcttct tttggtaaaa acaatagagg
     6061 atcaagcatc cctcgacagc tttcatctga agataacgaa ataaaattaa ttcgttcata
     6121 catagccaca aactctggta tgtgtccgaa tttttcaagt gcctttaaaa agaacttttg
     6181 ggtttcattt ttcggatcaa tataaagaat tttttgacct aaaaacatca gccataaaaa
     6241 aatatatttt acaaggaccg attttccttt acctggtgga ccaataatca acgtatttcc
     6301 attggtatgt tccgcgcctt caatactttt ctttgttaaa ttgggataat accatactag
     6361 tttactagat agcgctaacg cttctttcgc agatttaaac ttttttgcaa taacaatccg
     6421 tcctaaagga aatcctttct tatttcctac ttctttcttt aagtccaagc cataatctgc
     6481 cacaaaacct gtcgtagcga cctgctcgta actacgaaaa cgttgcgtac ttccaattaa
     6541 gcattgatta aacaacgtta gctgatccgc taccgatcga taaaatttga aatctgtaga
     6601 ctcaatcgca aattcaatat ctttgactcg ttcttccaat tcttcttttg aagaagcaga
     6661 tacaaccagc gtgacggtag aataacacaa tcgtttttct ttgcgcttca gcttatcttt
     6721 tacatccatt aaccgttctg taccaaaatt taatacatcg tctgaatcta aaatgccgtc
     6781 aacggtctca tattcttttt cttgttcata aatccgtcca gccattttat taatttttcg
     6841 ttcatcagat tttgaatgct caaatcgaag tcgaatatgc gtatcaattg gaaaagataa
     6901 gctatcctga agattttgaa taaagctact cccatacatg gctaaaggag cgtccgtttg
     6961 acttaaaaac gtcgaataat gcgtttgatt caactgttca atcgcaatgt atcctgcttc
     7021 attcgaaatg acgccctcgc aaatttccat tggcgtaagc tgtctttcag gcaatttttc
     7081 ttctgatcga tggaaaaaat aataataaat ttgtccaatc tcttgggcag ataagcgctc
     7141 tatcggctta taattggaca attccgtata gagctccttt tctaatgttt catacgttgg
     7201 taacacacta cttataggaa ctcgttgtcc cgtaatacga tagataaact ttttaaaaac
     7261 atgcttaagc aattgaaaat attcgatcgg atcagacact ttcatttctg tagtgaaacg
     7321 cacaaacaga tacgttcgat actcgtttaa ttgcacttcg tcttttaaaa tctttcctgc
     7381 ctgttcaaag tacactttcc caagatctgc gcattctccc tttaccaaat ctcgaatggt
     7441 ttcttcaatg tgcgtatcta ggttaaattg acgtggaata ttgataaaat gatagtcata
     7501 ttcatcatgt ccaagtatgc cttcgccatc ttgaagatag ttcttgaaaa aatcaatgtc
     7561 atttaaagga aatacttggg aagaaagttg atagcctacc cacacatcat catttttggt
     7621 caaaacaaaa tttcggataa tggcgtctat cggaaattct aatcgaggaa gcttcgtttt
     7681 tttctttttg aactgccaaa tcccgataat aaagacgacc aaaagaaaca aaaacgccat
     7741 ttactcgact cactccttcc atttgtgtag ggaatacgtc actttttcat ggaaaaacgg
     7801 cacatacatt tcatgttcaa agcgttgttt ccgtttctga taataggacg ttcgcccaat
     7861 taaatactcg aaaaagtgtt tattgtccca tttcattgaa aacaatgtcc aaacgagaac
     7921 accaatccca aaaatcataa tcaaccagga ctttcgaaac agtgtctgca agaaactaat
     7981 ttgattcaca tacgcaatca aaaataggat caaaagaatg aagagaaata ccccacctat
     8041 gactaattgt tccatgcgaa tgccattaga aatccaggga attttttgtc ctttaatccc
     8101 gtaaattttg tgagggtgct taaactcctt tgtatagttg tattccatgt tcactctacc
     8161 tccttttaca aaaaaagaag cgaccagaag ttctaatctt ctgatcgctt ttgcagtttg
     8221 tctttttctt atgataattg tatccagggc acaatcattt gcgttacccc cacgttctct
     8281 tcactatcct taataaagat aatgttcttt agccattcac caatatcaac ggcattcgat
     8341 aacaataaga cgagcattat caaaataaag acgccaataa tggcaaacgc tgcccgcgca
     8401 aagccttggg tcgcataagc accaaccatt agaacaatcg caacgattgc caatagccaa
     8461 atgacttgtc cagatacatc atcaaataac gatttaagat caaaaagttt catttccatt
     8521 ctcctctaag ttcattttat tcatttaacg tagtaacaaa ataactatcg ccacttgttt
     8581 ttacttccat tgaaaagttt gattggaact cgtttccttt ctcaaaatag aaacgatact
     8641 tcccttgcaa aaagtagttt ccttctttat tttttactgc atgatccagt tgtacattga
     8701 tcaattcgcc gccttctaac cctttttcgt tagaaatcaa tgccaatttt tcatcatttt
     8761 tgacatataa ctcaaaaaac cgtttcacaa atgcttccag cttgatttct tcgctttcag
     8821 tgaccgcttg cccagaagaa taaaatcgat tttcctgata gttcacagaa ttggtattct
     8881 tactctcaga aagattcaaa aattgaacgc catcaagtac ttttaaaaca ttgttttgat
     8941 aactgactgg aaaagaaagt tgactgcttt tccaatgttc tccttctgta aaagtaacct
     9001 cataaaatag gcgatacatt gcttgtgtca aatcgatttc ttctagttta ataaagcgaa
     9061 catctttcgc tactcgattg gtagccgtcg ttacaaagcc tagatcagac gcttccaatc
     9121 cttgtgctaa ataatgattg acggaagcct cccattcgtt tcgtttttct ggcgaataag
     9181 taaataacct ttcaataaaa tgttgtccct cgtacttcaa ttgttccgta gaagacaagg
     9241 tttggttgac ttcagttaac aatgtttctg gattgacttg ttttttcact gctaaacgcg
     9301 ttaatgtatc cactcgtcca aagtttagga ggagcacgac acccattaac agaaacaaac
     9361 aaaaaaaagc gcctgaaaat acactcatgg cacttttttg cgaaaattgg atcattcgat
     9421 ctgacggttc tgttttcttt ttttggaaca aaaaacgttt cttttgtttc ttttccttct
     9481 gttgtttcgg tggcttagtt tggaacgctt gtggattaaa ttttgcatta ctcatgagaa
     9541 atgccatacc gttctaacaa taatttcggt attttgtcca caatcactac ttcgtggaca
     9601 accacacttg ccccctccaa aaaggcaagt gcttctttgt aggcattttg actgtatttt
     9661 tttgataaag catgacaaat ttcttgataa aatagttgac tgcctaccga ataccgatta
     9721 ttgtgtatcc gccacaaaac gaacacactg atttgaatcg acgggcgtaa aaaagaaaaa
     9781 ttctttccat aagaaaaaga acgtccttct tgggtttctt ttgcttgttc tgttggtact
     9841 tcttgtacct ctcttattga ttcttgtctc tccataacct tctccttttc ttattcttct
     9901 aaaagctgtt gattaaagct acttcccaaa ttttgcataa acgtgatttt ctttgacgct
     9961 ggatcaaagg ttacgtcaaa aatattcatg gcattattcc aggaactatc accaatttta
    10021 ttgcgatacg acaccacaac gagcccttgg atagtcgctt tttgtgtgct ttttaaataa
    10081 acagaacatt tctctaattc agaagacacc ccgtttgaat caccatagga aacgccttct
    10141 ttgaaatatt gcttcatcag tttgtcagaa agatatggtg cacaattgtt ttttcgttct
    10201 tgaaaagaat caggctcata attaaacaga acctcgaacg cttctgtcac tgttttttga
    10261 aaggtttgaa tgtttccttt ttctgttgaa tcattgtttt tcaattcttc atttgctttt
    10321 tttaacgtcg tgacctcgct ttctacttgt tgaagttgtt ctttgagtga cgttacaagg
    10381 tcttcctgtc ctttcttctc ttcttggaac ttgtttatct ccaccgtcag tttctcttcc
    10441 tttgctgtta gctgtagctt ttcatgaata agaaaaaatg tactgcctac accaaataag
    10501 aacaatccgc ctacacaaac ataaataaat cgtttcttca tttgacacct cttatttctt
    10561 tatcggtcga gcaaaacaaa tatcaccacc attaagcaaa tacccaaagg ataaattgac
    10621 ttgcccttca cctacaccgt tagtattcat tcccccttgt tcgattactt tcgtagaaag
    10681 tccatgccaa tcttccgcta aaacagctgt gtgtccatca tttcccacac cagaacctaa
    10741 gttcacaatg ataatatcgc cagcttttgc ctcgctctca ggtatttctt gtaagtattg
    10801 cttacttcct cttgcgtcgc tcgtcattga gccggtaaac cacccaacgt tcgcaggcgt
    10861 tttataccct gctttcgtta aaacaagcca cacaaagctc gaacaatcgg catagccatt
    10921 tttatctgga ttttcgacac taccaaattg tgcccgttgc ggttgtgaat aatgaaaata
    10981 accaatcaga ctttttgcta cgtttaatac attactatcg gaagaactgc ttgttcctgt
    11041 tgtttctaga caataatcgg tcaagtcttt acttcctgcg tttgccccat tttctaatgt
    11101 aattgaagcg acgggatcac tgtcattctt tcctagtttt gcgtaccatt gattcgcaaa
    11161 agtaatacga ttccccatca ttggtatccc tgcacgctca aatttcactt gccactgttc
    11221 tgttgcttcc tctacgcttt ttgcttgtaa tgcaacttgt gaagcttttt ctgttgtgtt
    11281 tagttcatga atcaaaaact caacttgtac acctaaatct gaccactctt tgtttcgctc
    11341 tttggcaaaa tttttcaaag cagtcgttcg ccctgccgtc cattgtgcaa tcccatgccc
    11401 agataatgga tcgtttggtc gttcaatcgc tgtgggactc atactcgact cttgttgaaa
    11461 attgcctaac atacccgcta gaccctctaa tgtcgtattt ggaatatgtt tttgtacttc
    11521 ctttgcaatt tgaagcccgt ttgtctccat gccttggttc gttaatgaga tagtacttgc
    11581 gacaatttcg gttggactac agtcgatttc ttcctcttca gaaccactac taatcaaaag
    11641 tatcataaac agaaaaccaa acatacatag taatagtgtg ggtagacaac cgaacccaca
    11701 ccataaaaga agttttttct tcatgtttga tccttcattc gcttagttca ttttttgtgt
    11761 tgcttttctt ttgcgccaaa aagcaaaacc gcttcctgaa gccaataatc caccaagtgc
    11821 tgtcgcaacg agagacactt cttcgccagt cttaggcaat actttcattt ttccatcaga
    11881 ccctttcacc gcaatatttc cgcttggaag ttgttcgacc ttgtctgaaa tgggcgtcaa
    11941 gccatttcct gattctttat aagcgacacc atcaacgacg gagacaattt tttctcctga
    12001 agctgtttca attggtgtac ttggtgttgg aatatgggtt aattcagacg cattattggt
    12061 ttcaatttga acattttgtc cacctgtttg ttggctgcca atacttccat ctggttttac
    12121 tacgacgtcc tgtactggag gcgtagtgtt cgtcccccca ttattggtag gtggtgtcgt
    12181 tggaggagtc gtatttcctt cattgcctcc tggattcgac ggcttttctg gtgtctctgt
    12241 attaccacca tttcctgggt tttcctcaac gggtggaact tcaggtgttc cttcccctcc
    12301 gccagtatct ggattttctg gtacttcagg cgtttctgtg cctccacctg tatctggatt
    12361 ttcttccgtt ggtggaatct ctggggtttc aggagtttct ggtatttcgg gaatttctgg
    12421 ggtttcttct cctccatctg ttggtggtaa aacaggttct tcaggaactt caggtgtttc
    12481 ttccgttggt ggaataattt ctgtcggtgg tacacttgga tcacctggtg acgtagtatc
    12541 tgcatacgca ctggttccta ccatacctag caatacagtt gcactaaata aacttcctaa
    12601 aatcattttt ttcatgtgtt tttcctcttc tcttatttat tgatttattt caaaatcatc
    12661 tagttcggga agcgtggaaa ctagataatt tttttcctgt tcttccaatt gaaaagacac
    12721 tttttttgtt tcttgtgtct ttttctcggt ttccaatgta atgacatacg caatttgcca
    12781 atgcttgcct tctttcttca tttcccagga taaaatactt ttgacttttt ccttttgagg
    12841 actgatttgt ttcgcaactt tttccgttag aaacgattga agctttttcc tataccgttc
    12901 ttctgccatt tcacccgaat aataattcgt aataaaataa cgactaaatg tatcaatttg
    12961 tggttgtttt gtcgcaagtt cttccgcaaa tgcaatgcgt tcttgtaact ggtgctcctt
    13021 tttcgttagt tgttcaatct ctctttgcgt tgaaagttgc ataataaaaa gaccacaaga
    13081 tacaacgacg ataaatccaa acaaaagcca ttgaaaagac atttttttct tctgaagaga
    13141 gccgttgctt tgtgtaaacg ctgtttctac agaaagtggt gttttctttt cctttttgat
    13201 ttttgttggt cgacgttcgt tcttttggtc tttctttact tgataaaact tcggtaattc
    13261 ggaatttaag gtttgaataa aggtttgtgc ttcttcactt aattgcccat tttctgttaa
    13321 agattgttca atcaatgaaa tcaaatgaaa ctcttctact ggaaaatcat acgagccttc
    13381 aaatactgga atttcatggt tttctgtgta gatttccaag gtacaccagc caatttccgc
    13441 tcgtgccaat agctcttcat tttctctttt caattgaata aatgccttat ctaatgcaat
    13501 ttttttatct gttagtaaag acatgttatc aacttctgta aatcgaatca tcatcattag
    13561 tcaaacactt cttccaccac ttctaccgca tacaccactt gaatagggtt atttaaagaa
    13621 aatttcaccc gatattcttg attgttcaaa ctgacgatat agctatcctc atcttcagca
    13681 taataggtat attcgttgcc tttgacttga aaatattttt ggatcgcttg tctttgttgc
    13741 cattcgccaa tctttggaaa ataggcgaaa tgatagccaa ataaaacgcc tatccctaat
    13801 aaaacaattc ctgttactat aatcatctgg tttcctcctg tacctgttta tcttctattg
    13861 actcttttgt cggtaagcaa taaagaacca gtaacacaag agagccaatc gttgggacaa
    13921 aatagaaaaa aataaatgcc caatgaaaac ctgcgtctcg taaacgtcga acgctgagag
    13981 aaagagacgg gacaagaact acgaaacaat acacaatcaa tacgcctatc aaacaaccaa
    14041 ttattgtttc agaagcattc aacgctcctg ctagtccaat cgctaagcct aggccaaacc
    14101 caatgagggc attcattaac gcaacgagcc aatactcttt tcttgtggct ttccctttgt
    14161 aattaaagct attctgccaa aattttttat aatttgtgat catatctttg tcctttctaa
    14221 tgtttatttt tgattctagt ttcttctgtt ttactaacaa tgaaacttgc cataaaaaag
    14281 gtggccaata caccaccagc tagaaatcca cttacaaata acaaaatttt ttcctccttt
    14341 ctaaaaaatt tcgccataaa aaggcaaagt atgaacacga taaataccat gtacatactt
    14401 tgccttttta tgcaatatat gttaactctc caagcaatta ctgattttta aattctttat
    14461 aaataaaata aaaatcgttt gagtaaagaa ataagtcaat gaagacccaa acgataaaaa
    14521 atattcctaa tactatattt aataaactac gttctagata gatacgagat ttcaaattaa
    14581 aaaacgaaaa tgagagatac aaatatatca aatatttaaa aagtaaccac attttctata
    14641 ccactcctta tcagaaacag ccttattctt tagaataaga ctgtttctgg gttgattaat
    14701 ttgtcttttg ttaaactacc atctaaggca agttctaaat gtaaatgtgg tcctgtacta
    14761 tttcctgttg aacccacata accaataatt tgtccttgtt caactgtttg tcctacgttc
    14821 acgcaatatt cctgttggtg cgcataaaga gccacacttc cgtcttcatg ctcaattgtg
    14881 acataatttc ctgacaaagt atgatacgaa gcttctatga ccgttcctgc cttgctcgca
    14941 tagattggtt ctccttgatt ggctgccatg tctaatcctc tatgaaattc ttctcctcgc
    15001 caaccgaagg aactagaaat tgaataattt tttagaggta ccatatagcc ttctttgctt
    15061 agttgtggac cagctacttc tttatcatat ttgattaagt cataggtttc gattaaccca
    15121 tttaattttt tatcgtagct agtgtctgtt gcgtatcttc ctgttaaaaa tttagtcgct
    15181 tcctgatagg tttttgcctt acttttccaa acaccatcat aaaaagaggt atttccaact
    15241 aagccttctt ttaacagttt ggcatagtct tttaagcttt cttcatagct ctcatattgc
    15301 cgaaaagttg cttgggtcgt ataaagtgtc ccattgccta aatcttcttg tgtcgcaaaa
    15361 gagacgccct ttccgttatg tgtgcctttg attccaaata gattgtagtt tggtgcctga
    15421 gccaattgac tttgtccgct tgcggattct aaaatggctt gcgcgatcat aacagaagcg
    15481 tataaatcat tttcttgtcc aattttacga gcagattcac cgattttacg gataaacgtt
    15541 tctacagacg cgtctttttc aaaatgaata gtcccttcgt tatcttgtgt gctggaaggg
    15601 actaattctt gtatcggtgc gcttggtgct tggtggtagt tttttgtctc ttctgaaccg
    15661 attgattgtg cctgcggtac gtcattcata ggtacagtca cactctgttc aggtgccttt
    15721 tgtgggcgtt ccgtttttgt tggcggttcc gcttctgttt cggtacttgg tttttgagga
    15781 gtctgtgagc tttcatcaga aggttgctca tttggttctt ctgacggttc ctcctcaggt
    15841 tgttgattgt cttctggttt gtctggttga ttcggtgttt ctggttgttc ttcagaacct
    15901 gttggtggag cgttcatttc tggattatct ggtgtatcta ttgtatcttc agtcggtgtt
    15961 tctggttgat tttcttctcc agtggaattt gttgtcgttt cttcagtgat cgttgcagat
    16021 tgcgaatttg tcagttgatt gttgccttgt tcattagcca aaaccgctgg cgtataccca
    16081 aatacttgac ctactaataa caaagcagat aaacatttac tcgttttttt cacaagattg
    16141 cctccattct tcaatcaatc atctagagac agctgattgc tgtctctagt catactttaa
    16201 ttgctttgtt tcattttacg acgaaccaga acagccccca caccaacaaa aagaaacagt
    16261 cccaatgcaa ttaacacatc gttcactgtt tctcctgttt ttggtaagct ttgttttggt
    16321 gtgctcgtcg gcgtttgttt gacaggggtt gtcggcgttt cttttttaat gccttttgct
    16381 gtcaatgttt gtttttcatt cttcaaatca aagtgacgtg cttcttcctc tttatggtct
    16441 ttatccttat agacaatatg agttgcgacc aatttctccc cgtcttttaa cgtgcctgct
    16501 ttgtagtcaa aggatacttt aaactcttca gacgctttag aaactttacg tgcagtttct
    16561 accgtaccaa cgactgtatc ttttccattt ttatcaatct tatggaattg agtcacccag
    16621 tatttggttt cccctaccgt agattctgga aattcttgcg atacatgatc gatcaactct
    16681 tggtctttgt ctggatcaaa tgtttggtcg ccattcactg taataaagcg tgtttcgatt
    16741 tttggttctt tcgcagtcac tgtttgatct ttattggtta aatcaaaatg acgagcgaat
    16801 tcttcttcct cttctttgtc gttatagaca atgtgcgttg cgacaatcgt gtcaccgtct
    16861 gaaaccatgt ctgctttgta gttgaacgtc acatcaaatg tttctttttc atcggtcact
    16921 tttcgtgttt cttttaccgt atctaataca gtgacttttc catcggcagt aattttatgg
    16981 aattgcgtca cccaatattt ctctgtccca accgcagatt tagggaatac ttgggacact
    17041 ttatccacta attcttggtc ttttttcggg ttgaaatctt ttccacctgt cgttgtcaca
    17101 aacaaagtag aaatttctgg ttggtcttct tctggaacca cgatttcttc gtttttcaca
    17161 gtaattggtt gtacttctcc gtctttgacc gttactaaaa tcgtttctgt ttgtcctgac
    17221 gcttgcccat aatgctcagg gaacacaatt tccgtaaatt tatacgtacc gtctggtaat
    17281 tttagttttt ctggttcttc ttttgtttca tacacaaagg acacattttt attatgtgca
    17341 gtcatacctt caatcaggaa tttcgctcct ggtaattgta gatgattgat tgaatcgact
    17401 ttattgaagt ctacttcacc cgtttttaac aagttgtcaa tcacatctaa ttcaaacgta
    17461 agcacaggcg tcgtgttatc gccataggcc acagagaacg tggtttgttc ttcagaaggt
    17521 tgacggtagt tgtctggcac atttttttca atcaattggt atttgttttg atcgccattg
    17581 tagaattttt tgtttgcctt ccagtgtccg tctttgtcag tttttactga atccaagact
    17641 tctccttttt tcacaaggac tgtttcttta tctggcgcag taatatctgt tttggcgata
    17701 atatcaaatt cgatattttc tgcaggaacg gtatcataaa caggtttgcc ttgattccag
    17761 gattcttccg cattatagac ttctttgact ttctttcctt ctggaatgaa ctgttgtaag
    17821 tcattttttg cagttaacgt cacttctttg tttggttcaa tcgtaatcgt atgagtttgt
    17881 ttgtctagaa tatacccagc tggtgctttg acttctttga catccaattt attttgaaca
    17941 tattttccgt ctttttcata gaatgcaggg atttctttta aggcgatttc ccctttggca
    18001 tccgttgtat attgttttgt ttctcccgta tcatgacgtt tgatttcata cgtcacattt
    18061 ggaacttttt tccctgtgac agcgtctagt ttggttagtt tgaagtcgcc attttttaga
    18121 actttaatag ttagtccagc tctattagga tctggcacat aaagctttcc tagattttga
    18181 gcaacaggat ctacataggc aaaacttgaa cctacccctc tagaatttgg atccagtgaa
    18241 gaaacagaaa ttcgtccgtc atttgagttt tgatttgctt ttataaacaa actatttcct
    18301 gattttctta cttgaacgtt agctgtgttt tcaacaaccg acccatccca ttgagaaaac
    18361 acaccttttg tatccttaac ttctacttct tcccctactt taaccgtcac tgtttggcta
    18421 tcaaaagaag gatcttcaag taaattgttt atttttctat ctacagtctg tttgaaagct
    18481 tcataattag attgtccaat agttccataa ttaacaaatg aaccacctaa aacttcccaa
    18541 atatatgttt gtgctaatac atagttatcc attgaaccac tagaatagta gccaaaataa
    18601 ccaattaaag aaatcttttt tctaatagca cttgtcgtaa ttaaattttc gagtggtgtc
    18661 acatcatatt gaatacccgc actcgtgatt gcgccttctt gaatgcaaaa aacatcttct
    18721 ccgtcaattt gccagcgaac catgtaatca tgagtataca ttgttccttc aggagtaatt
    18781 cgagtcatat tcacatcttt acgaccattt tcagtcaatg ttagattggt agcacgtgtc
    18841 tgtgcattct tttccaagat tgcttgccat tcttcagatt cttgcgccaa tcgttcttgt
    18901 tcagaaaaag aagtcgcttt ttgaatatct tcaatggtta taaagtattt ctcataacct
    18961 tcagggacct cttgagtcat tacattgggc ggagaaacat cttctgtcgt gtcctgcgta
    19021 gttgtctctt ctgttgtttc acttgtggtt tcagtactga tcgcttcttc actacttttt
    19081 gattcttcag tcttcattac ttcttcagta gaatttgatt catttatcgt ttctgcaaat
    19141 gccgttacag atcctaacat gggattgaga aatgtaacga aaattgccaa ccaagttgcc
    19201 attcttttaa acatattttt ttcgttcctt tcattttatt tttcagtctt ctaaagtaat
    19261 ttcttatgga cttcgacacc gaaacatttt tatcctcctc aaaacaaaaa aagactagaa
    19321 cttataattc tagtcttcat caaccactta cttttctttt atttttagta gaaatctaca
    19381 gtgtgttttt gtgacccgtc actgtaaata ataacatcca ccataaaacc tttgttgtac
    19441 cactgcgaat ttggatcgct aacctctgca ttagcccaat cataggcttc atctacagtg
    19501 ttaaatacct ttccactatt accaatctct ccaactacaa ttgttggctc ttcaggtgtc
    19561 gttgtgccac catttccgcc ttgatcgcct ccaccaggat tgcttggttc ttcaggcgtt
    19621 gttgttcctc cacctgtgct tgggttagaa ggagttgttt ctcctccagt atttccacca
    19681 ttacctggtt gtgtaggtgt ttgagtagtg ccaccattgt tttctggtgt acttggcgtt
    19741 gttgtaccac cagttgttcc tgtttgatct ccagctggtg ttgttggtgt cgttgtattt
    19801 ggtgtcgttt gtgtatttga agtcgttcct tgtcctactg gttgattcgg atcaactttg
    19861 tctttctctt gaatcactgt ttgagccaca gtttcacctg cgctgttttg tacacttgct
    19921 acattgccat taaatactaa tttatttcct gcatagatta aatccacatt tgtaatctta
    19981 ttcatgtccg ctaatttttg gacagtaata tttgttgctt gactgattgc gcttagggta
    20041 tctccccaga caattgtgta ttcattccct ttaatttctg ctttgatttg ttccgccgaa
    20101 cgaggtgtcc aaactttgtt ttctgctgct tcagcattcg ttcccacagc aagactaact
    20161 cctccgacta cgattcctag tgccaatact ttcaacattt tcattgtttt tcgcctccga
    20221 tttgaataaa tttatttaca aaaacatagt accaaactta tgtctatcag tcgatatatt
    20281 ttttgtagat ttttttattt tttgatatac taactttggt actaaaataa ctaaaaggaa
    20341 tgtcacacat gcaacttaat aatttaaatg aaatacatga ttgggctcaa aataatttac
    20401 ttaatcgtaa acaagctgcc aaaatcactg gacaaaacta tgatgctttt tctagctctt
    20461 tacgtagaaa taatatcaaa ccatttctag aaataggcga cgaacctggt ccttccatca
    20521 ttcgcttata tctaagagaa gatattgaaa aatacgcaaa acagctcgaa gaaaaaagac
    20581 agaaacgtca atgaggcgtt ttctgtcttg gatttacaag taaaacaata ataaacactt
    20641 ttacaacgga ggttctatga ataagaaata cattgtttca gttggttcaa tcgtactgat
    20701 tgtactcgct attattgctt tttctgtatc caacaaaaca gaaacacagc cacaaaatac
    20761 ggttcaatcg actactacaa gtcaatctag cgatcattcc gtctatcctt cactaaatag
    20821 tttaaaagaa acgcatggac aagatttaat tgatcttggt tcttctcaaa ttactactgt
    20881 cttatttgaa aagaaaaata ttgcgtttat tactcctaaa gaaggaactg accttgaatt
    20941 ttctatcaaa gtaacaaata ccaaagaaaa tttattctcc attcctgaca ttcaatatac
    21001 ccctaacaag ttaaagattg gggatacgtt tggtttggct aaagtaaatg aacaatactt
    21061 tgcttactca ctgaaataag tttctaatag caagaggaaa aaccctcttg ttatttttat
    21121 cacataatca ttttataaaa acgcttcgaa aatatcaaca tttttgaaga aactattttt
    21181 ctaatttctc aatttcttcc aagggatttt cgctaatgac ccatacaggc acagattgct
    21241 ctcgaggctc cccttcttct aaccaccaac ctgcataatg aacttcttcc tcctcagcat
    21301 taccatgata ccaaccaaca aaggattcat ctacaacgaa tgaaatttct tcttctggat
    21361 caaataattc atttttccat gcttcaattg cttgttcttt ggtgtacttt tgtttgttaa
    21421 atacaccaca aatttcgtca tctacataaa aaatttcaaa atcaaaatta ctcgttcctt
    21481 ttactggcat cttaactttt cctcctttga ctcacttcaa tagatatacc actcatgttc
    21541 attccattgt ttactagtta attggagttt tatcttaggg ctttgtgcac tctttccaaa
    21601 aacttgagcg tcgttttttt cttctaacca aaaaaggtaa tttcttacat tcggtactaa
    21661 atgtttttcc tgcatttctt catgaaaaac atataattct gcttcatttt tcaaccaatt
    21721 cattattcat tccctcattc acttcttcat accgccattc agatagtgag atttcttttt
    21781 gtttctccac ttgtcctata aaaatcgctg tattcaacaa gctgatcaca cttaataata
    21841 aagcaatgaa actaatggtt acacttactc tttcacattt tgtcattttg tttccttctt
    21901 tactcgtttg ttatttcttg taataggtct aataactcct tcaccgaaat ttgcaaatgg
    21961 ttcgcaaaaa tttcaataaa agcactcgca cacattacca gaccttcaac tgatatatca
    22021 ctgcgacaat aaaattctgg agaaacactg atttctgatt ctttcttaca attgaacgaa
    22081 aaaactattt ttgaatcaaa ttccggtaat tcttccacaa ctatgttttg ggattctacc
    22141 aagaaaggga gtgcctgttc taatgcttct tctggtgttt gagcagatag aataattgct
    22201 tcttgttctt ttatttcatt gttctcaaat actttataac tttttttcat tgttagttcc
    22261 tctttcattc ctctctcaat tagctaagcc gttactggta cgccattctc taaatttagt
    22321 atcgctaatc cttaatgctc gtcgaatatc aatcattcga taacctattg cacgaagttc
    22381 tttatatctt ttagcagtta gcgtctctaa cgtcatttca ggcaatgggt tcgaatcaaa
    22441 aatagaattc gaaccgatta tttgggccaa ttgttgtact tgtaacagct cttctgaact
    22501 taattcagtg attgctcgat tgcctatcat tgcatacatt ttctgtcgaa tatacttctt
    22561 ttcttgttta gacaacattt ctatccactc ctattttcat tatcatttgt tacctccact
    22621 tcagtaaatc cacaaataaa tgaattcata ttccaagtac cattcccttc aactacaact
    22681 tttctcctgt cctctttcgg atattcaaga atcagaccgt ttaccaatat gtttttcact
    22741 actactaatt tgtttgtata ccgtggaatt gctgttccga tatacttcac tttatttcct
    22801 acgctaattg tcatttcctc actcctattc ttttttatcc aaaacttttt cctgtcctat
    22861 tcctgccaac gaatatactt cactgcatgc ttaaacaaaa caaaatgttg ccctgtttta
    22921 tttccgtctt ttacttgttc cacatacagt tcatatttgg tcacaccttt taaaacgcca
    22981 gatacttttt ttcatccatt gtcacaatta gttttttcat caatcgattg atactgcact
    23041 gctcattttt cttgttcata tagtattcct ttctttttca aaactagcct cttttgttta
    23101 ctagttttca tcaatcatca acgatttaac agattttttc tttcgcaggg tttctagcgt
    23161 cagcaacccc aaactctaaa aaagtgcctt tgctcccttg aaaaacgagc ggacgattgt
    23221 actcgtcatc atatgttttt catactacct gatccacgca aataaccttt ttttgttctg
    23281 tgccacaccc atccatacgt atcgcttgag attcaccata cttttttagg tggtttatcc
    23341 ttcctaagtc cctgttagac acgctgatta acttagaaag tcctgtttgt atccaatttt
    23401 caaagatctc ccaaaagaaa taagtaggtt tacgtgtatc gcttctgttc aaagtacctg
    23461 tttgtacttg agtatttcta ctcccctcgt tcaagggatt ccttccacca gctagctttt
    23521 cctttcatca tcaaatgttc ctgaaaagca tttacgtttc gtgtccgtgt gcttacttat
    23581 tcttttttcg atagcgatta tccgctcgct actcgctatt ttatactttg tttgttagct
    23641 caaataaaaa ctaacaaaca aaaaaggcta gtttctttcc gttttatttt tttaactgtc
    23701 gataaatagt tgttagttta attccaagtt ctaaaaccaa tacgcccaac aaaaccacag
    23761 gaaaaacaat tggggcaaac aagtaaaatc ggatagtttt catgtcttcc ctctttcttt
    23821 ttggtgtcgc tgtggtcgtt cttgccgttc aatacttcga tacatttttt attcattatt
    23881 tgctcgtttt gttttcctct ggactgcttt tcttgctttt tgaatacttt atacgctttt
    23941 cgtgcctgaa ttaataggtc ttgtcggcgt ttcattcgtg agaaaacaat atttgaaacc
    24001 atcaaatcct ggttcgaaca accacgattc gcaataattt tcccatcaaa aaaaatagtt
    24061 ttcacttgag aaaaaaacag ttggaaatct tgttttgttg ctaatttttt tgtcaaaatc
    24121 actgtgcttt tccctgttga atctacaatg attcttcgaa caatccgatt cgataacttc
    24181 caacgttgcc gattaaacaa tgttacttca caacaaatat gacgtaagtg attaggggtc
    24241 attttcatgt gtccaattcc tccaaccaat tatgcatcga aattttgggc aattgtgaca
    24301 gcccataaat ctgcgtttcc aggtcttcat aactacaaga acgcttggct tgaagtaatg
    24361 aatattctgc ttgatctgat agttttgttg ccattggatc tagattcaat tctcttacac
    24421 aacattccca aaaattcaat agcgctctaa aataaaatcc ttttcgattc tgaattgctt
    24481 ttccttcatg acgagcaatt ttttcatttt gaatacaccg ttgatacgtt ttgcttaaat
    24541 cttctgcaaa tgtttcccct aaaattcgat actcgcttcc atcagataaa ccatgtgttt
    24601 gattatactt tcgttgccgc ttttctataa ttcgtttact gtcgtatatc atgtttagct
    24661 gctctttcgc ttcacgccat tcagctgaac tgcgaacaat ccatgtcaaa acatctttcg
    24721 gaacaaacgt actttcgctg taatgagcaa taaactcttt ttgccaatca tcagccattt
    24781 caacaggact agtcgtatcg atttttgtat ctagattttc tgtatctaga ttgttagaag
    24841 aattattaaa taattgtgca gaattttcac atggattttt ttcgtcaaaa tcatcgaaaa
    24901 tcgctaaatt atcaacagat tcctcctctt cttttccttg tgcagaatct acactggttg
    24961 agtcaacagg acctaatgaa tacacatcat tttctgtcgt atctaattct cctagataca
    25021 accgagcttt gtttcttttt cctgtttgtt gatcaaatcc catatcctct atttctagta
    25081 gctcatattc tttcaattct tttttgattg tattaacaga acgtctgcaa caattcaatg
    25141 cattggctaa ttcttgttcc gtaaattcaa aataaacaaa accttgacta tctacttgat
    25201 ttcgttgaat ggctaaatct aagcgtgacc gaaaaaccat atacgcgatt tttgctttat
    25261 cagacaatga gcgatatttt tcacttgtaa taaatacctt tggaaattga tagtataatt
    25321 cattgtattt acgagttgcg gtaaatcttt ttgttgccat cgttattaca cctcatcttg
    25381 tccatatatt aagaaagaat tttggtgttg gtagacacga acatcttcca ttgtagcaat
    25441 caattctttt agataaattc gaccgaattc aattgttagt aagtcgtaat cttctaattc
    25501 atttttgatt tcttcaattt cttcttcaga cacattgagt aaaatggcta attcttgatt
    25561 ggtataagaa atatagacat gtttcttttc atcaatccgt ttttcaatta aggattcttc
    25621 taagtactca ttcagtaagc tataggcgta ttttgcttgt gcagtcatcg cataatattt
    25681 tgacgatttt ataaagaata gttttggcat atgaaaagtt gcttgtgtca gcacatctgt
    25741 tgcgcgaaat ttgtttttta ccatattttt tcctcctaga aaaacacgca aaaaaagctc
    25801 tgtataaatc gaattacaca gagtttcttc ttgttgatat ttaatttcta aaatacgttt
    25861 cgggtaaaaa ctaaacactt actaatattt agattgtaga atcttcgcct tcatttattc
    25921 attttaaaga gaaaaagatc aagtgaaatg tgcccaaaag tttgattcta ttaat
//
LOCUS       DACECZ010000028        24823 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.028, whole genome shotgun sequence.
ACCESSION   DACECZ010000028 DACECZ010000000
VERSION     DACECZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.028"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(369..1076)
                     /locus_tag="IWO35_001092"
     CDS             complement(369..1076)
                     /locus_tag="IWO35_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316162.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(1141..1977)
                     /locus_tag="IWO35_001093"
     CDS             complement(1141..1977)
                     /locus_tag="IWO35_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0316163.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1995..3404)
                     /locus_tag="IWO35_001094"
     CDS             complement(1995..3404)
                     /locus_tag="IWO35_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0316164.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3418..5241)
                     /locus_tag="IWO35_001095"
     CDS             complement(3418..5241)
                     /locus_tag="IWO35_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0316165.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6084..6410)
                     /locus_tag="IWO35_001096"
     CDS             complement(6084..6410)
                     /locus_tag="IWO35_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316166.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6456..6818)
                     /locus_tag="IWO35_001097"
     CDS             complement(6456..6818)
                     /locus_tag="IWO35_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ0316167.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6855..7070)
                     /locus_tag="IWO35_001098"
     CDS             complement(6855..7070)
                     /locus_tag="IWO35_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0316168.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7197..7532
                     /locus_tag="IWO35_001099"
     CDS             7197..7532
                     /locus_tag="IWO35_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0316169.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7608..9605)
                     /locus_tag="IWO35_001100"
     CDS             complement(7608..9605)
                     /locus_tag="IWO35_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316170.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEIRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9703..9978)
                     /locus_tag="IWO35_001101"
     CDS             complement(9703..9978)
                     /locus_tag="IWO35_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0316171.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10210..10944
                     /locus_tag="IWO35_001102"
     CDS             10210..10944
                     /locus_tag="IWO35_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0316172.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            10983..12125
                     /locus_tag="IWO35_001103"
     CDS             10983..12125
                     /locus_tag="IWO35_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0316173.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12220..13611)
                     /gene="sufB"
                     /locus_tag="IWO35_001104"
     CDS             complement(12220..13611)
                     /gene="sufB"
                     /locus_tag="IWO35_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ0316174.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13616..14086)
                     /locus_tag="IWO35_001105"
     CDS             complement(13616..14086)
                     /locus_tag="IWO35_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ0316175.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14073..15308)
                     /locus_tag="IWO35_001106"
     CDS             complement(14073..15308)
                     /locus_tag="IWO35_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0316176.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15305..16591)
                     /gene="sufD"
                     /locus_tag="IWO35_001107"
     CDS             complement(15305..16591)
                     /gene="sufD"
                     /locus_tag="IWO35_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ0316177.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16604..17377)
                     /gene="sufC"
                     /locus_tag="IWO35_001108"
     CDS             complement(16604..17377)
                     /gene="sufC"
                     /locus_tag="IWO35_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ0316178.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17574..18422)
                     /locus_tag="IWO35_001109"
     CDS             complement(17574..18422)
                     /locus_tag="IWO35_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0316179.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18446..19132)
                     /locus_tag="IWO35_001110"
     CDS             complement(18446..19132)
                     /locus_tag="IWO35_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0316180.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19125..20159)
                     /locus_tag="IWO35_001111"
     CDS             complement(19125..20159)
                     /locus_tag="IWO35_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0316181.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(20554..20892)
                     /locus_tag="IWO35_001112"
     CDS             complement(20554..20892)
                     /locus_tag="IWO35_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ0316182.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(20894..21250)
                     /locus_tag="IWO35_001113"
     CDS             complement(20894..21250)
                     /locus_tag="IWO35_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ0316183.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21256..22443)
                     /locus_tag="IWO35_001114"
     CDS             complement(21256..22443)
                     /locus_tag="IWO35_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0316184.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22634..23479)
                     /locus_tag="IWO35_001115"
     CDS             complement(22634..23479)
                     /locus_tag="IWO35_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0316185.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23713..24678
                     /locus_tag="IWO35_001116"
     CDS             23713..24678
                     /locus_tag="IWO35_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ0316186.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         7251 a         5221 c         4221 g         8130 t
ORIGIN      
        1 gaaacaatta catatgattt tttcataaaa aaacagtact gtctagtagt ataaaatata
       61 caaatttctt aaaaattttt tcactaaaat cactacctga aacagtctaa aacacttata
      121 tatcaacgtt taatgaaact atctccgttt catttcagat acaaataaat atagtcgggc
      181 tccaaatccg aaaaaaaata cccccagtag cttatcctaa gctactggag atgtataagt
      241 acggtcatat aacaacgggg tagggtaaat aaatgaaaaa gatggaagaa tcattattca
      301 ataacttttt taaatacttc gttgtaatca gttacctgtc cgttcttaaa taccacagaa
      361 taatcttttt acccattttc aagtaaccga agtggaatgt ctttaccaac ttgctctgtc
      421 actgcattac tgatacctgt tagattagct aataaatctg ataaagattg agcatctgta
      481 gaaatctctg tatcagtatt tatcgtgatt cctttatctt catttacaag cttccaagta
      541 ggtggtatag ctgtgtatgt ttcgatgaat gaatcaattc tttcatctat cgtcttccca
      601 gtatctactt ctgtctcatt actcttagat gaatcgattc tttcatccat cgtctgctcc
      661 gtaactgctt ctgtctcatt attctcaccg gaaactctct tcacacccaa acttatatat
      721 tgtccatctt tagtgtgcat agttaaaatt cccacgcttt tactaataaa ttcactataa
      781 ccagttcttt caatagagac ttcaatgtat ttcacactat catactcaaa agaaaaatag
      841 acatgtaaaa gcattttgtt gtttacatct acataataat cctttgactc tacttgagac
      901 cttcctatac ggaatacagc tttactgtct gatatctcta taccagcatc cttattatta
      961 atctgccaat ctccaagtag ctcttctggc aagctagtag tttcttgtga acactttacc
     1021 acttcactgg tagaggtcga aacattcacc tccgcactgt tagaagaatt ttccaaggaa
     1081 tcaggagcct tagcacaacc aacaagtctt tatatattgt gcagccgata ttttcttcat
     1141 tcagtacacc cttgaaatat gcagtgttag ataaagcagt tcgtcctgat ttgctgaggc
     1201 acccatctct tcttgaacgt actccttgat tttcaaggcc gcccgatact ctttcggaaa
     1261 acgctgcttc actgaagcca gcaaaaaatc atccttctcc ggcaactctt ccttcttaat
     1321 cattcgggca ataaaaaact gtaaatgcgt cacaaatctt aagtaaagca tcgaatcttt
     1381 atcaaccttt ttttcgggaa acagatatcc tagcatagcc aagatatcct ttagtgctct
     1441 taccgagagc atggtttctt ccatatttgc ttgttcactt tgggcattta caatgtgaaa
     1501 cgcaatgttt ccagcctcct cttctggcaa ctgaacattc atttttttgt tgatcaattg
     1561 gatagcatag cagccgatct caaattcttt tgggtaatac ttttgaattt caatcagcag
     1621 gcgattttga aaaacaatgc ctttttgact gcgttcaatg gcatgcttca agtgatcaac
     1681 caaggtaaaa aatagacttt tattcagctt aaagtcaaac ttggcgttgg cataatcgat
     1741 aattcgcttg acgcaaataa ttagatcatc cggagcagcc tcaaacagtt taatgtaatc
     1801 cttttttaga gcatcagaaa gtgaatctaa tttaaatact ttttggattt tgtcagccgg
     1861 aaccaaatcg cctttcttta gttgaaatcc aatccctttc cccatcaaaa ttacttcttt
     1921 gccttgctta tctaagcaaa gaattaaact gttgtttaac gttttaatta ctctcattag
     1981 attcaccgtt ctctctaagc taagccattt cttgcgatta ctttttgata ccaaaagaag
     2041 ctttcttttt ttattcgttt taattcttta agattctcat cggttcggtc aacatatata
     2101 aaaccataac gcttattgaa gccttgatgg gtactaacaa catcaatcgc tgaccaaggt
     2161 aaataaccaa aaacatctac accatctgtt aaagctgctt gaatagccag aatatgtttg
     2221 gctagataat caattcgatc ctggtcatta attcgtctat cttcctcaac tatgtcgggt
     2281 gccccatagc cattttcagt aattaaaata ggtaaatcat agcgatcgta caatttcctc
     2341 aatgttaatc gtagtccagt agggtcaact acccagccat atggcgtttt atctacataa
     2401 ggattttcag ctgcccggta gacaccttct tctcccaaca tgatctgttg atcccccgct
     2461 ctagcggaaa catctgatcc gtcatttttg ctggcagcga tggtggcagt tgaataataa
     2521 ttaatcgcaa taaaatcggg tttaccggag gataaaattg cctgatcctc tgcctcaatt
     2581 ttaggatata atccacgatc attcaaatag ttttcaaaca aataattgta ttttccccga
     2641 actgctacat ctaagaaaga ccagcctctc aatgtttccc aattatgggc tgcaattgca
     2701 tcttctggac tggaggtagc ttgatacata gccgttaaat tgattgctgg accgattttt
     2761 cctttaagat tcaactgatg atacaagtga attactctag cttgagctaa taaaacatgg
     2821 tgattaattt gaaagagttc tttttttgaa ggcagctctc cacttggtgg caaaccaatc
     2881 gctccaggat gaaggatcat cgtgttttgc tcattaattg tcagccaata gttcaccttg
     2941 tcaccatact cagtaagcaa tacctgtaca tagtccacaa aataatcgat agttgctcga
     3001 tttaaccaac cgccctgctc ctctagaaaa taaggtaaat cgaaatggta catggtgaca
     3061 atcggttcga ttccctgatt caggcattca tcaattactt gatgataaaa agaaattccc
     3121 ttttgattgg ttttattatt ttcaatatcc ataattcgcg tccaagcgat ggaaaagcga
     3181 aaagctttta agcctaattc tttaaacagc tgtatatctt ctttatagtg atggtaaaaa
     3241 tccgaggcaa cttcaaaatt agctacccct tctgtatgct gatacttgtc aataatagac
     3301 ggttgtttcc catcctcctg ataggcaccc tctgcctggt aggcactaat ggaggcaccc
     3361 cataaaaact tctctggaaa tgctgaaagc tgtttgtatt gcacaaaaat cctctcctta
     3421 actaatttgc atcaatggtt cgtgttcctt taagcttccg cttgcttcac taattcctgc
     3481 gtaattttca ctgttggtca ctaccacaat cactgtatca tcgtagccag cgtttctaat
     3541 ggcagcgaaa tcggcgttga ttaataattg tcctgctgac acctgatctc ctgcttttac
     3601 aaatacttca aagggttccc cttttaaatt cacggtgtcg atccccacat ggatcaataa
     3661 ttcaatacct tctttagaac gtagaccaat cgcatgttta gattctaaaa tagtaatgac
     3721 ttctccctca acaggtgaat aaatgctgtt ttcttctgga tgaatggcta cgccctttcc
     3781 caatagtccc ttagaaaaga cagcatcgtt cacttcagaa ataggacaca cggttccttt
     3841 taccggattt tcaacaatga ctgcttcggc tattgccgtt tgctctgatt catcagaagt
     3901 atcgtcaaat ccaattagat aagcaatgat aaaggttgca acaaaggcga ttacacacgt
     3961 aataattgcg tgaacaatat tcattggatt ttctccaata aaggctggta aggctgctaa
     4021 gccaggggaa acaaaggcat atctgactaa accgctcaag ccagcataca aaccagcaat
     4081 tgcaccacca atcatggaag caactaatgg ttttttcagc ttcaaggtta ctccatagag
     4141 tgaaggctca gtaattccca ttaaagcagt gatcccagtg gacacagcta gctgcttcaa
     4201 ctctttgttt ttagttttcg cggctacagc aaaggtagca cctgattgag caatattaga
     4261 agccagcatt cctggcccat tgatcatttc agaacccgta ctgctcaatt gcatagtagc
     4321 gattggcgtc attgcccaag ctgtacccgt aaccaccaaa aatggctgca aaccacccat
     4381 caaaaatgga attaaccaag ctgctttgcc gttaatccat tcagccccca ttgctataag
     4441 atcgttcagc aacgttccaa acggtccaat gacaaccaac gctaaaggcc ccataattaa
     4501 aaataccagc atcggcttag taaagaattt aataaacgaa ggcgaaattc gatccgcaaa
     4561 gggctctaca taggacatga tccaaacagt aaagataatc ggtaaaactg atcccgcata
     4621 atctgcagct aataccggaa ttcctagaaa agaaacggca tcgccagtag aaaaaagctg
     4681 tgaaatattt ggatgaagca aaatgccagc aacagtaatc gccaaaattg ggttgacatc
     4741 aaactttatg gctgccccat aggctaatag tactggcata aaataaaagg ctgcatcagc
     4801 aatagtattc aaaatgatgt aagtttgact actgtctgat agtacaccag tcaaagtcaa
     4861 gacggccaaa atggctttta ccatcccggc acctgttata gctggaatca tcggtgtaaa
     4921 ggtggtagaa atgacactaa tcaatcttga aataatactt tcttgattgt ctgaagaggg
     4981 gcccttttta attgaagttc ccacttcctt ttgaatttcc ttgaaggctg tttggacttc
     5041 attgccaatc accacttggt attgtccacc gctatccacc acactaatta caccaagtgt
     5101 tttttctaag gctttcgtat cggctttcct tttatctttg agttcaaaac gcagtctggt
     5161 ggcacaatgg gttaagctga ccacattttc ttttccccca acctgttgca atacatttct
     5221 tcctacttct ctgtaatcca ttattctctc tccttaaagc agtaaaaaag accaagtcaa
     5281 accacgcccc aatcaacatt gaagtgttag tttgtcttgg tcttgcctgc ataatcagta
     5341 acatgccaat aacgttattt tgctgtaatc caataatagc acattatgat agcgcaatca
     5401 acagggaatt taatttttta tcttttcgtt tgaccgtata gggtaatata acagttagat
     5461 cacatcaact agggtatatc tgcgtatact tactgctaaa ataagtatat ctaatattaa
     5521 ctataccagt aaaaccattc ctacataacc agcgggaaat tcccaaaatc gtgaaagcat
     5581 caaaaatgca tataacggat cacacaactt caacaattct gctcttggga aaaattgatg
     5641 aatttggtga gtaaaggatg gaataaacag cattccaaca atataggcaa caatagtgat
     5701 acttgttttt ttggtaaaaa tcagtaagca atagccaatt tcaatcattc cgagatataa
     5761 aggtatcaat agaatgattt gggctagtat tcttaaaaat taactagcaa atgtttaaac
     5821 tgatttattt ctaatctaca tgtagtaagt atttcaattc caccaattat caataaaaat
     5881 accaaacata tccataataa ttttttcttt ttcatttctt ttatattgtt ataacagtga
     5941 ataacataaa aattcccgac aatcattgta atattttttt gtcacctgta ttagtattta
     6001 atttatagga aactataaaa actccctagt gaatttacac ctttgggagt tcctgtttgg
     6061 acggtatgca taatacattt aaatcaattt aacattaaca cctcagcata tggtatctcc
     6121 acatatatac ggtctttctt tggatagtac tcaatattta tgttgatagc acttccattg
     6181 tctaatatag ctttagcccg gtatgtgtta tctccatttt catgagtaat cgtcacatca
     6241 tcacatgatc gatttaagtt gtactgttct tgtaaaatct gtgttaccaa tggttttgat
     6301 gacttctcta atccagatgt acctgtcatt tttgaaaaaa taatacctat aatagccaag
     6361 acaacaatac ctataatgac aaacttcttg ttcatattta ctttattcaa ctttttatct
     6421 cctatctcca aaataatttg ttcttatccc tcttatcagt acattgacat ccctaaaagt
     6481 acaccaaaga ttgctccagc tacccctgct atcatcaaaa tcgtacccat tttctcatct
     6541 ttttctcgat ataagtagcc cataataaca gcaacagcac caaatacaac aggaataaat
     6601 gctaacgata ctaccataga aatccaacca ataatcaaat acagcttccc tgtctcttgt
     6661 ccatgtacga tttgttcctt catttcatta attgtctgct cagtactttt ctttactgtg
     6721 tctgttccgg ttgtctcatc tgtttcttgt agtgctccac aaaactggca atacttactt
     6781 tcatctgtta tttctttgcc acattttcta caatacatgt cttctcctcc taaaatcatt
     6841 caattgctta ttagttaact gtttacttta ttaaatacat cagaaaacag ctcattcatt
     6901 ggtacactta atatctcaga aagcatcttc atttgatagg gtttaggaat cgttctccct
     6961 gtttgccacg aacttacaca ttgctgtgta acattcattt tttttgctaa ctccgtttga
     7021 gaaagtcctt tttcttttct gtatcggctg agattactta cttttttcat gattctcccc
     7081 tcctgtttta ttatatacaa caatatgatg tatttcaact cataaaatat aaaaaacttc
     7141 aaataccatt acaaatacaa caacttgttg tactatactg agatgggggt gtattaatga
     7201 aacagaaaca atatgcaaag gtcattagag aaaagcgaaa agctctcggg tggacacaaa
     7261 aagaattagc tgaaaaaatc ttctctacac aacaggcagt agcaagatgg gaaaattctg
     7321 taacagagcc taacctcgat agcttaacag ctctttctag agccctagga acccctgtaa
     7381 gccattttct agacaatgtt gtcgtagatt atgaggaaga gtttttggct ctctatcgtt
     7441 ctcttagcac tgaagatgca gttcgaacaa ttgactatat gaagctatta aaaaggcaag
     7501 aaaatgaacg caatcagcta ttgaaagatt gaaaaataaa taacccaccg aactttaacg
     7561 gtgggtgtat tgagatgaaa taagattaag agctatggat aacctgtcta gtttaataca
     7621 attgggtctt ttctcttcct tactctccgt ccatcatctc ttgttacact tgtaaattga
     7681 tacagaggaa ctatcccagc tcctgataat atcaattctt ttattgtttc atctatccct
     7741 gcccctttcg ctaaatatat taaaaaacta ctacttgctt tcacattgta tagtttctta
     7801 atatccttag aagctcgttc aagagtgaat ccttgtttaa caaagtagcc tctgtttttt
     7861 attaaagcat ttttcacgct gttatcccca acattacgta ggcgttcaca aacagtgtta
     7921 ataagttctt tctctgtcat tagatatgtc ccttgccacg ataagccctg taaaacttca
     7981 tgtgcttcat ctaaaatcaa ttgtttttcc ttgctgagtt ctgatcggga attttgagga
     8041 aatactcttt tagcctcttt ttcgttaaaa catatcttaa cccattcata agaaaaatat
     8101 tgatatgtaa aatgaatttt ttccagaagt ggaaggatat cttcattcaa tttttgaatc
     8161 acagaagttg cattaaaaac gtctaatcga tagacattgc ttctcttatg attaaagttt
     8221 cctaacgctt tctcttcctt taacctattt aggtattcga gcatcttcaa aggaacatct
     8281 tcatcatcta atttttttat caatcctata aaagtcataa gtgatagtaa cttattacat
     8341 ttatctatgt cataagtttt accatagtaa gaactcattt ttttagctaa ttgttccccc
     8401 gacaaaatac aagtcacact ttccttacta tcatcaaaaa aattaacatc agaaagcaag
     8461 acatcataga cacgtcgtat ttcctctaga taattcctta ttgtcctgaa aagtttgtct
     8521 ttcattgatt caagagtgtc aaaaagtact tttgattcct ctttcaattt tcgaaaatgt
     8581 gtaggataca agtctaggtt ctctaatagc caattaaatg tttctacccg actgtctttc
     8641 tttaaaatta gcatcacaac tccaataaaa ttttcactga tatcacaatt actggaatga
     8701 caataaaaaa tggaatgccc agttttactg gtaaaaatac tagcagaggg attatgatcg
     8761 ttgtgaaaca gacatcttaa gtttttactt gacttaagta atttattcat atcaatggtt
     8821 aacaatcttt cataaatctc attaaaagac atgtcggagg aatcaaacag gcagtcttta
     8881 aaccactgtt tcatttcttc tttgtcgtca ttttttatca acttaacaaa atcggtttca
     8941 ttgactagtt catactctct ccctgaatca ctttgacaat ttttagaaca tgtcatggta
     9001 aaatcaatcg gaagctcagt aatatctaac atgttgtctt cattaattat tatcactgca
     9061 tctgacttat atcctccaaa aaacattcta ttcgaagcat ttacattgtc atcacacccg
     9121 gggatgttct ttttcaagta ttcgtaaaca gcaaccattt taccatagtc agtaattgct
     9181 tcttttaata gaaagacaat cttgtatttt tctagatttt cagagtaccg aatacttttt
     9241 tgtatcgcaa acgcagaatt atgaactaaa ttgatagata aaaccttttc tatgcttaag
     9301 tatctactat catctttact caaatctact accttcccct tctcatcttt aacagtgtta
     9361 tcgacatcca gaataattac tctggtttcc acaaaactct ccttgtcatt gcctttattc
     9421 tttgtcttgc ttggaataaa tgattgtgat gaatttagta tctctacaaa ctcatctctt
     9481 gtaacttctc tatagttatc taaacaccta tctcttacat cagttttttc tttttttgta
     9541 actggcttct ctaataattt ttcttgatct acataaattg gaatattaat cattgatgtt
     9601 tccatttgtg tttcctcctt ataaaattct gttaaagact aaaacataca gataataaaa
     9661 acaaactctt tattactata tgttttagtc ttatttgttt actcaacctt tataccgttc
     9721 catgaaacta tcaatatcag attttttaaa tctaatgacc gtatcaattt ttataacagg
     9781 taacccgtat tgagaaacat attttctaat cgttcctact gatacaccta gatattcact
     9841 tgcctgctga tagtccatat actctagagt gactccagaa ataatgtctt ctagcatagg
     9901 ctttagcact gtaattatct ctttcacttg ttgttcaact tgtatagaaa caatgctatc
     9961 aatgatttgt agagacatat ttccccctcc tttcgtgatt gcttgaacaa attttctaag
    10021 aattatcttc gtattttttg ctcaaaataa aagtattttg ttattaaccg attaatcaaa
    10081 caaaaaatga ttaccagttc atcgacatac acataatacc tcgataaaaa aggggtgtca
    10141 acagctttgt aaaatttatt tacaacaccc cttttttgag gtatactaat tttaggaggt
    10201 gtggtcaata tgataaaacg tccaccaata aattatttag aacgaaaaaa gatactcgga
    10261 acaaaaataa aagctataag aaagagtaaa aaattaacac aaccagcatt tggtcttatg
    10321 attaacaacg gtcaattaat tgacaaaaaa actatctacg aatgggagaa gggaacctat
    10381 ttaccaatac cagagcgatt atcccgaatt gctgatttgg gaaatatgag tatagaggag
    10441 ttagtttgtg gcaatgtaga agaatatatt ttgggaatta ttctttatag agatagcatt
    10501 gtactagatg gtataacatt tccagataaa aacctgttcc agcatcttcg tcaacagttc
    10561 ccaccagtac actctaattt agatacatgg ctggatagat attccaaatt agaaccagag
    10621 atgcaagaat ttattgctaa caagacttgt aataaagtta aaaatgaaaa aattagtctt
    10681 ttcaatatct taaaaataga agaattattc atcaatgcta ttgtagaaga atttgacaat
    10741 aacattcttt tcctcactag cagtatagaa gaactactag aaagaatggt agatgaatgg
    10801 ctacctattc aattgaaaga catgtcttat cccgaagaag ctgtgagaga aattactgat
    10861 aatataaaca agctagaaca aacgatttca tcaattggga aaaaatacac taagaaaaaa
    10921 atgaagggag gtgataccat ctagtggtaa gtactccttg taaactaaga ggagatgatt
    10981 taatggcaaa aatatcaaag tacaaaaaga aaaacggtca agtggcgtgg atgtataaag
    11041 gacacattgc caccgatcca cgaactggtg aaaaaataaa tactacgaga agaggtttta
    11101 gtagcaaaca agaagctcaa aaagattatg acgagtacag acatcaaata ttatacggtg
    11161 ttaaaaagaa agcacctgat atgacatttg aagacctgta taatgaatgg ataactcatc
    11221 agcgtaccag tgttaaagct tcaacaatcg caatctcagt aagatacgca aataatcaaa
    11281 tacttccagc atttggtaag cttaaactat cgaatatctc tgtcccatat tgtcaaaaag
    11341 tggtagatga atggcacagt aaatatgaaa gttacgatta tatgagaaaa cagactgcac
    11401 aaattttacg ctacggggtt gcaatgcaat atattgacaa taatcctatg gaaaaaacac
    11461 ttcttccacg aaaaaaagag tatgagaaaa atcgtaaatt ttactcaaaa gaggagctta
    11521 ataatttact ggatgctttt aaagattttg gtaacatgaa acaatacgcc ttcttcagat
    11581 tattggcata cacaggtatg agaaagagtg aagtactcgc tttacagtgg aaagacatag
    11641 acacgtttaa taaagaacta catgtcaata aaactcttgc tgtagatgaa tttggtaaag
    11701 ttataattca atctccgaaa acaagagctt ctagacgagt tatctcttta gatactgaga
    11761 ctctttctat cctcaacaac tggaaattac agcaaaaaga agagtattta aaacttggat
    11821 acaatactag ctctaaagag caacatgttt ttacaacagt taaaaacact ctatacattc
    11881 caaacactgt taatgattgg ttaagatata ttttgaaaaa atataacttg cctcgtatta
    11941 cacctcatgg gtttagacat acacatgcta gtttattatt agaagcagga gaatctgtca
    12001 aggtagttca acaacgatta gggcatgaaa atagtaaagt gacattagac atctatgcac
    12061 atattacaaa taatgcacct aagaaaacag gtcaagactt tgctgatatg atggctcatc
    12121 agtaaaagag ctggtcaaaa tctggtcatt tctagtatac aggcaaaaca aaaaagctag
    12181 aacccttata tatcaagggt tctagctcct ataacaggtt tacccaacac tgccttccat
    12241 ctcataactg atcaatcgat tgagttcgac tgcatattcc attggaagtt ctttggtgaa
    12301 tggttcaaca aagcccatga cgatcatttc agttgcttca gattcggtca ggcctcggct
    12361 catcagatag tagagttgtt cttctgagat ttttgagact ttggcttcgt gttctaaaga
    12421 gacttggctg ttgtggatct cattgaatgg gatcgtatcg gatttcgacc attcgtccat
    12481 gatgatcgtg tcacattcga tatgagagat cgaacccgca ctgtttttgg caaaggttac
    12541 ttgtcctcga tagtttactt ctccgccgtc ttttgaaatt gatttagaga cgatagagct
    12601 agatgtattt ggtgcattgt ggatcatttt tgcacccgta tcttggattt ggtttgctcc
    12661 tgcaaaggcg atggagagca tggtgcctcg tgcacctttt ccatctaagt aaacacttgg
    12721 gtatttcatc gttgtttttg cacctaggtt tccgtcgatc cattcaacgg ttgcgccttc
    12781 gtatgctttt gctcgtttgg ttactaagtt gtacacattg tctgaccagt tttggatcgt
    12841 tgtgtaacgg caatacgcat cttttcttgt aaagatttct acgatggcgg catgcaggct
    12901 gttacttgaa taagtaggtg ccgtacaacc ttcgacatag tggatgcttg ctccttcgtc
    12961 aacaatgatc agcgtacgtt cgaattgccc cgtgttttcc gcattgatcc ggaagtacgt
    13021 ttgtaatgga acatcgactt tgacgccttt tggcacgtaa ataaaggtcc cacctgacca
    13081 tacggctgaa ttcagcgctg ctaatttgtt gtcggttggc ggtacaagtt ttgaaaagta
    13141 ctctttgaat aattcagggt attctttcaa agcagagtca gtgtcagtaa agacgattcc
    13201 taacttttcg aattcatctt tcatattatg gtacacgact tctgattcat attgtgcgga
    13261 agcaccggct agataagctc gttcagcttc tggtatacca atcttttcaa atgtatcttt
    13321 gattttttct ggtacgtctt cccagtcacg agcgggtttg tcacttggtt tttggtaata
    13381 tttgatggca tcgaaatcga tgtctgaaag gtcggggccc catgtttgca ttggcatttt
    13441 gttaaatgtt tctagagatt tcagacggaa ttcaagcatc cattctggtt cgcctttgat
    13501 tcttgacatc tcacggatca cttcttctgt caagccttct cctgtactga aaaccggttc
    13561 gacatcatcg tgaaacccga atttatattc ttctaattct ggtacgccca tgttctcact
    13621 cctcttcctt gtggtaatga tggaaagctg cggtaccatc gttttccatt gcttttccta
    13681 gtgctttcca ggcaagtgtc gcacatttga tccgagcggg aaatttagct acaccaccaa
    13741 gcatagcagc atcgcctaat tggtcgggat ctttgacttc ttttccttgg acaagctgta
    13801 gaaagtctac agttaaatgt tctgcttctt ctattgattt tccgatgacg gcgtctgtca
    13861 tcatcgaggc acttgccgta ctgatggagc agccactgcc gtcaaaggcg atatttttga
    13921 tgattccgtc ttttacatcg acttgcaatt cgatcacatc tccacatgtg gggttgttca
    13981 attcgatctt gcttgtcgag tcagtcaagc ttccacgatg atgcggatgg gaagaatgat
    14041 ccaagatcac ttgacggtat aaattgtcta atttagatag tcccatgttg gaaaaactcc
    14101 tttgttgcca tgattgcttc aactaaacgg tcggcgtctt gcgttgtgtt gtagaaataa
    14161 aagctcgcgc gagcagtcgc tgatacttca aggtacttca atagcggttg cgcacaatga
    14221 tgacctgctc tgacggctac cccttccatg tctaaagctg tcgcgacatc atggggatgt
    14281 aacccatcca aattgaatgc aacgactcct gtccgaaaat tcgggtcttg tggaccataa
    14341 acagtcaatc catcaattgc ttgtaatttt ggcaacacat aagcggctag ttcttcttca
    14401 tatcgatgga tattgtccat accgagtttt tgcaaataat cgattgcatg gcctaatgcg
    14461 atagcgccgg cgatatttgg tgtccctgct tcgaatttcc aaggtagttc tttccatgta
    14521 ctgtcgtata attcgacaaa atcgatcatt tcgccaccaa attcaacagg ttccatttct
    14581 tctaaccatt ttcgtttacc atataaaaca ccaatacctg ttggtccgca cattttgtgt
    14641 ccgctgaaag caaagaaatc acagtctagt gcttggacat caatggccat gtgcggggca
    14701 gattgagcac catctactac gatgaccccg ccgttttcat ggacaagttc agctaattct
    14761 tgaacaggat tgaagacacc taagacattg gatacatgag caatggagac gattttggtt
    14821 ttgtccgtaa tctttttcgc tgcatcctcc atatccagga agccttcttc tgttagttcg
    14881 atatatttca agacagcacc tgttcgtttt gccaactgct gccacgggat aacattagaa
    14941 tgatgttcca tgtaagagag cactatctcg tctcctgctt tgatatagcg gtcgccgaag
    15001 gttctggcta cccaattcaa gcttgtcgtc gtaccacgag taaacagcac ttctgctgtt
    15061 tcttttgcat ggatgaacgc ccgtactttt tctcttgctt gttcataatc atgggtcgcg
    15121 cgttctgcta acgtatggac accgcgatga acattggcgt tatcgtgttc gtaatagtac
    15181 gctaatgcct ggagtacttg ttttggcttt tgggtcgtcg ctgcattatc cagataaact
    15241 aaaggttcat cattgacgtc ttgaaataag ataggaaaat cattgcgtag ctttttagca
    15301 tcgatcatcc attcaacttc ccttcgatta cttcgactaa ttctttccga acttgttcta
    15361 cagggattgc tgttaagaca gaaccgagga atcctcgaat cactaagcgc tctgcttctt
    15421 ctttatgcaa tcctctactc atcagatagt acatttcttc tggatcaact cgtcctacac
    15481 tggcagcatg tccagcagtt acttcgtttt catcgatcaa aagaatagga ttcgcatcac
    15541 cacgtgcttt atcagaaagc atcaagacac gactttcttg ttgggcatct gcaccttttg
    15601 cccctttcaa gatgtggccg ataccattaa aggtcaatgt tcctttttct cggatcactc
    15661 cgtgttgcaa aatatgcccg atcgtatgag gggctttatt ggttactcgt gtatcgattc
    15721 cttggatttg tctgcccgag ctgattgcca cgattttgac ttctgcgtgt gcaccttctc
    15781 ctgctaaatc tgagtcaaag tcagcgatca catgaccgtc attcatgaca ccaattgccc
    15841 agtcgacaga agcatctcgt aaaatgtgcc cacggcgatt catataagaa gtaatatttt
    15901 ctcctaattg atctacggct gaatatttga tttttgaacc agctttagcg atgacttcca
    15961 cgatgatatt acctgatgat tttgctactt gttctttggt tgtctggaaa cgttccagat
    16021 aagaaaattc actatgttca tctgccacga tcaacacatg cttgaagaag tgctcatcgg
    16081 atgcgccatc ttgaatgaat aaggactcga gtggttcatc gatcacgaca tttttaggca
    16141 catacaggaa aataccgctg ttcataaaag ctacatggag tgcagtcaat tggtcttcgt
    16201 tcatgtttac tgcttttttc atataatatt cttggactag ttctggatat tcgatcattg
    16261 cagtgaacaa gtcggtaaaa atgactcctt tttctgctaa ttccacaggc agttgttcaa
    16321 aaatcgtcca tgaaccttgt tggacaagca caggattgtc tttcattgca tcaaatgccg
    16381 gaatcgtgcc tgtttcactg atggtttgtg tttctttcac atcaaataac gaccagcgat
    16441 ggaatttcac ccgatcgata tgcggcaatg gcagttcatc tgctttcgct agtgcgtctt
    16501 gacggaaggt tctcatccaa tcaggttcgc ctttacttaa agaaaaatca tttaccgcgt
    16561 caagataatc gagccagttt ttttctttca ttcttgctcc cccttacgct tcttcttctt
    16621 tatagtcgat tcctaattct ttactgatac cagcgtagcc ttctgcttcc agacgtttag
    16681 ctaggtcagc attccctgtc atgactactc ggccttccat catgatgtga acgacatcag
    16741 gtgtgatata gttcaataat cgttgatagt gggtgatgat caatgcaccg aaattgtctc
    16801 cgcgcatttc atttactcct ttagcaacga ctttcaaagc atcgatatcc agacctgaat
    16861 cgatttcgtc taaaattgca aatgtaggtt ctaacatcaa caattgcagg atctcattcc
    16921 gtttcttttc tccgccagaa aaaccttcgt tcagataacg ttcagccatt tcttctggca
    16981 tatttagtaa agccattttt tcatctaact ttttcaaaaa tgccataaca gaaattttgt
    17041 cgttttcatc acgtttcgca ttgatcgctg cacgcatgaa ttccgcattc gtgatacctg
    17101 gtatctcact tggatattgc atagctaaaa ataagccaag tcgcgcacgt tcatctactt
    17161 ccaagtctaa aacatttttc ccgtcaaaca aaatttcgcc ttctgttact tcatagtttg
    17221 gattgcccat gatagcggca gataaggtag attttcctgt gccgttaggt cccatgatgg
    17281 catggatttc tccggttttc attgttaggt ttacgccttt aaggatttct ttttcctcga
    17341 tcgacacatg tagatttttt atttctaaga cagacatgca ttataccccc aaattctctt
    17401 tcgttctcac tcattttaga ctaattgaga atgaagcgca atgaccttcc ttactaaaat
    17461 caaatatctt ttatttatta ttaaacaatt ttgatttcta aattagaata ggtataaaaa
    17521 agatgagaaa taagccatta cagacttatt tctcatcttt tttgttccat accttatttt
    17581 gcatcagcct ttactggttt aacagagccg ccccatttgt ctgtgatcca ttcttggact
    17641 tctttgcggt gaagcacttc aatcagtttt ttgattttgt cactgtcttc atcaccttta
    17701 cgagtcgcaa taatattcac gtaaggagag ttgtcctttt caagcaatac ggcatcttct
    17761 aatggattca accctgcgcc gtaagcgaag tttgcgttga ttgcaactag gtcaccttca
    17821 tcattttggt acgtagaagc taaaagtgat gggtcgatcg tatggttgaa cttcaagttc
    17881 ttagggtttt cttcaatatc atcgaatgtt gctgtttcca gatctactcc atctttgact
    17941 ttgactaagc cagcatcttg tagaattgtg atgatgcgtc cccagtctga ttcagaattt
    18001 gaagtaatga ttgttgcacc atcttttaat tcggaaacat ctttgacttt tttagagtac
    18061 aagcccattg gttcgatgtg gacagcaccg gcattgacaa agtcatatcc tttttctttg
    18121 atttctttgt tcaaataagg aatatgctgg aagtaattgg catcaatgtc gccttcttcc
    18181 aaagattggt tcggtaatac atagtcatcg aatttgacga tttcaagatc gattccttca
    18241 tctttcaata atggtttcac atgttccaaa atctctgcgt gcggtgtcgg tgaagcgccg
    18301 actttcaatg tcgtgtcgtc tgctttattt gctgaatctt tgctgtcgct gttgttaccg
    18361 catgctgcta atcctacagt cagcaaaagc gctgctgcaa atccaaaaat tttctttttc
    18421 atatcctaca tccccctata tcctattatc ttttgtctgt tttcttagtt aagaaatcac
    18481 cgattccttg aacgataaat acgattagta aaataattaa agtcgcaacg aatgtcactg
    18541 ttagattacc acgctggtag ccttcctgcc aagcaagacc tccaagtcca cctgcgccga
    18601 tcgcgccggc catcgcagta aatccgatca tcgagatcgt tgtaactgtt aatccagaaa
    18661 tcaatgcggg aatactttca ggtatcaaga ccttccagat gatttcccag taacttgctc
    18721 ccattgcatc tgctgcttcc agtacaccag ggctgacttc acgaaaagca atctctacta
    18781 gacgggcata aaatggtgct gcggataaaa caagagctgg aatggctgct tgcgcaccta
    18841 gcatagtgcc tacgattgct ttagtaaatg gaatgatcaa aaccatcagg atcataaatg
    18901 gcgttgaccg gaaaatatta ctgatgatcg atacgattcc ataaatgatt cgagcatacg
    18961 gtttgttttt gcgtccaata gagtaaagga tcaatcccaa aactaatccg atgatcgtca
    19021 ctaagaccat tgagattaat gtcatatata gtgtatcaag gatagcctga ttcatcgttt
    19081 ccgggttgat ttgtttaaaa tctagatatg tttctgaaaa gcttttatcc acgatggatc
    19141 acctcaattc ctactccttg ttctttgatg aacacttctg cttgatctaa ttctttggtt
    19201 tcaccagtca tcaagacagt caatgaacca aaggaacctt ctttcacttg tttgatcttt
    19261 ccatacacaa cgttgacatc gatgttatag cgtcgaatcg cttgggaaat gactggctcg
    19321 ttggcatttc tttcgctaaa ggtcaaagtt gccaaacggc cgcttggatt ttctttgatg
    19381 agttcagcta atagttctgt cgaatcagct tctggttcga tgtcttgttg tacaaagcgt
    19441 ttggttactt cttttttcgg ttgtctgaag acagtcaata cgtctccttc ttctacgact
    19501 ttccccaact ccatcacagc gactttgttg cagattttgc ggatcacatt catttcatgt
    19561 gtgatcaaga cgatggtcaa attcaatttc ttattgatcg ctaacaataa gtctaatact
    19621 tcttctgttg tttgcgggtc caatgcgcta gtagcctcat cacaaagcag gatctcagga
    19681 tcatttgcca atgctcgtgc gataccgacg cgttgtttct gtccgccgga aagttgtgaa
    19741 ggatatgctt gtccgcgtcc ttctaacccg actaaacgca acagttcttc tgcccgttct
    19801 ttacgtttgc tctttggtac gccagctaat tccagcggca gctggatatt ttcaagtaca
    19861 gttcttgacc ataacaggtt gaaatgctgg aagatcatac cgattttctt tcggaatgta
    19921 cgtaattctt tgttttttaa ttttgtaata tcttgatcgt ttacgatcac ttcgccgctc
    19981 gtgggaagct caagtccatt caggagacgg actaatgtac tttttcctgc gccagaatag
    20041 ccgacgattc cataaatatc ccctttttca acgtttagtg aaacgtcatt gacggcttgg
    20101 atgtttccgt ttttcccatt gaatgtcttt ttgacattcg ttagttggat taatgacatg
    20161 ccattcacat ctttctctta tgatttttca aacaaaaaag ccttcgccct cttgccttaa
    20221 gcaaaaggac gaaagcttga acttccgtgt taccaccttg atttaccgtt acttcgcaat
    20281 aacagcctct tccagtactt ggacttagct tctttccgta tactgtcgcg ctgtaacggg
    20341 cactcccgta gtaagtttgc ttacgctcgc ttactctgct ctaagaccat cttccatctc
    20401 attttcgtta cccattttca gcaaacaggg ctctcttcaa acgtccgtga catgtactct
    20461 tcttttcaaa gctttctcgt ttcatatagg attgcttatg attatagcaa ctggaagaat
    20521 cagtgtcaac ttttttcaag tgattttctg attttataat tcgtcaaatt cttttggatc
    20581 aacatctttg aaacttaaga tccacgcatt catttcatct gaatcattca agatattgat
    20641 atcttcaccg attttatcat taacagaaga aactgtaccc gttagcgggc tggcaaattc
    20701 attgaccgct ttttccgctt caacttcgat cagaggttca tcttttttta gcgattgtcc
    20761 tgcttttggc agattggcaa acgtgatgtc tccaagttct tcttgcgctt catttgtcaa
    20821 accaacacag tattcttttc cattaaataa gatccaaagg ttgtcctttt tcttcaaatg
    20881 cttgcctgcc attttatatc agttctcctt cataaatttt ttcattgaat ccaattgcaa
    20941 caagctggtc ctctcgtaag atcagtggtc gcttgatcag cataccatct gtcgataaga
    21001 cttggctcgc ttttgaagcc gtaaactcgc ctacttgatc tttcagtcca agttcccgat
    21061 atttcatccc gcttgtgttg aacaggcgac ggattggtac ttgactcgtt tctagccaag
    21121 tctcaatcgt tgaggccttc ggtacttctt tgatcatatc aacggtttga taactaacgc
    21181 catgctggtc taaccacgct ttggcttttt tgcatgttga acatttcggg taccagtaaa
    21241 aggtgtacaa ttttcttacc tccttgaaga tcttgcaggt gcttcatttt ggtatctcat
    21301 cgataatatc aacccgatac cgatcatgtt ccctaaaatc gaggatcctc cctgactaat
    21361 gaaaggcaat gggatccctg ttaaaggaag taatccgata tttgctccaa tattctcaaa
    21421 gacatggaac agcagcatca tgatcaaacc gcttgcgata taggcgtaaa attcattatt
    21481 cgtatcgaaa cacacccgaa tcatacgata gattaaaata aaatacaaga aaatgacgaa
    21541 tgcaccaccg ataaagccga agttttcacc gataacggta aaaatcatat cggactcacg
    21601 aacaggcaca tagatattgc ttacgttgaa tcctttacca aacaagccac cggtaccaat
    21661 tgccaaaagt ccttgggccg gctgataact cattcctgac gtatcatgga aagggtccag
    21721 ccatgaatca atacgtgcaa actgataaga tttgaatcca actttataaa gcaagtctct
    21781 tccgccttca gtcgtaacca agaagatcgt tcctgctcca acgactactg ccaagattac
    21841 tacaggtgcg atgatgcgcc aagagattcc agacatcagg aaaatcccgc caaagatcgc
    21901 aagaaagaca agcatcgttc caaagtcatc ttgggccatg atcaatgcta aaaccggtat
    21961 tgtcacgatc aagagcttcc caatcagcca aaagtccgat ttcatcgtcc gttcttttac
    22021 ttgcgtatta tgtttagtta cgatcagtgc catcatcaag atgtacgcaa tcttcatcaa
    22081 ctctgctggc tggaaagtaa acgcaccgaa tcgaaaccag tttttagacc ctgttgaagc
    22141 agctaaattc tgatcataga atttgagcaa gagtcccatt accactaacc ccaaaccata
    22201 aaggtaagga gtcagtttcc agatccattt ggaattcaaa tgcatgatga caacgattgc
    22261 tgccgctcca actaaatacc agacaacttg catccctacg cctttcaaga ctgctggatt
    22321 gttcggatca tggctaagtg ctacatataa tgagagcaga ccaatcaaac ataacagaaa
    22381 gactggtaaa atcacgccat aatcaatacg attatcgttc gaagctttta attttttttc
    22441 catgtttcat cccttttctt aaaaaccata ctgctgatta ttatagcggt ccagttagat
    22501 aatggaaagt ctttccatag acatttggga aaaattaact ttccgtttgt gcagttgctt
    22561 gagcaaatgc agtacaaaag cctactgctt atctttagtg tatcatagaa ctttttttga
    22621 tacatgcaat tatctaccag ctgagattct cctcgctgtg agaagtttct gtgctttcat
    22681 tgtatcgaag gatctcttcg gcaatctcta gcagctgatc agaaaccaga ttggttcgtt
    22741 ggatgaaata aaaatatcga tggaaattca acggagtcat tggcacattt tctggaacag
    22801 agcgcttaga caaaatcgag cgaccgattc cttccttcag caacgcaaca attacatcgt
    22861 tacttttcac gatcatcttc ttatcttgga tattcatctc ttcaaaataa cgctgcgtgt
    22921 aatgaaaaac accagagttt gcttctctta caagccacaa tttgcttttt gggtccccag
    22981 cgatgactag ctggtcttcc atcaaagagt atctttgtac gccggaagtc tctaatggct
    23041 tttcgataaa accaaaatcg gattcatgtt tcgctacttg ttctaatgat tcatgagaat
    23101 tcgtgatttc gatctcaaaa tgcactttag gaaacctttt gatcagatgt ttcatcaact
    23161 gaggcaaaag atagacagca aaggtgtggg aagcaacgat ccgacatact tcttcttcat
    23221 tttgttcatt gagcaatttt tgctgagttt cttcccattc ttctaacaaa accagtgtcc
    23281 gttcatataa taagtcggct tgcttagttg gaagcatctc ttttcttccg ttacgttgaa
    23341 aaagatcgat attcaattcc cgctccaact gtttgatttg attcgagact gctggttgag
    23401 ataaaaacag tacttctgct gctctggaaa aatttcttgt ttcatatact gctcgaaaag
    23461 tcgttaataa cttaaacatg gcaccacttc cataactttt tatgataatt actataaaaa
    23521 ctatttgatt taaatataca aaaaaccatc tataatagaa atgtacaaca aaacagttgc
    23581 tgatccaact agtaacttag atgaagacaa tcagctgtct gcttgctcgt aatcatacat
    23641 tcctattcat tttacaatgt attcgttcat cttggtacta aaatctatta aataaaaagg
    23701 agaatattat atatgaagaa ctttttgaaa attattccga tcccgatctg cgggttgatc
    23761 cttgggttag cttcccttgg aaatctgttt aaagactatc accatgttgc tttaggcaat
    23821 ctaactggcg gtatctctat gattcttatg atattcattt ttgttaaatt gtttatctta
    23881 ttcgaacaca cgaaacaaac gttgcatgat ccaatcatcg cctccgtttc accaactttt
    23941 acgatgtcac tcatggttat ttgtacttat tttgtttcat atgaaagtat tgcaccaatt
    24001 gtcaaattca tttggctgtg cgctgttatc ttccaagtga tccttgtctt ttatttcaat
    24061 tatcaccatg ttatcaaagc tgatctttca attgaagcaa tttatccaag ctggtttatc
    24121 atttacgttg gatttggggt catcactgta actgccggga acttcttccc acttattgga
    24181 aaaattttct tttggatttc attgatctgc tatgcgttct tattgccgat catcatcaaa
    24241 cgtgtctttt tcgtaagaaa tatggctcat ccgacacgtc cgctgattac gatcgttgca
    24301 gcacctggtt ctctttgcct aacaggttat cttaaaaact ttgctgaacc taatttttat
    24361 cttgtcgtag cattgttcgc tttatctcaa gtattgtatt tcgttgtgct gtctatgctt
    24421 ccacgtttgt tcaagcttcg tttttatcca agctatgcag cctttacatt cccgcttgtt
    24481 atctctgcaa cagctatatt tactactgtt cattacttca acagtttagg tatctatagt
    24541 agcgtattgg atttcttaaa agttgcggaa tgtctgtttg caagtgtagt tgtcttctac
    24601 gttcttgctc attacgtacg tttcttaatt aaagaacata gcaaacatgg aaaatttgtt
    24661 tctaaaaaaa tcgcttaata atcgattcaa taggttgtga aaaagctgtt ctgcatcaag
    24721 taataagaac ggccaaacaa aaacagctgt tcatgttttt gtactggttg ggcttattac
    24781 cgcagatgca agcttttgaa caccgtttac caagaggtcg taa
//
LOCUS       DACECZ010000029        24297 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.029, whole genome shotgun sequence.
ACCESSION   DACECZ010000029 DACECZ010000000
VERSION     DACECZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.029"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            51..464
                     /locus_tag="IWO35_001117"
     CDS             51..464
                     /locus_tag="IWO35_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0316187.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(922..1197)
                     /locus_tag="IWO35_001118"
     CDS             complement(922..1197)
                     /locus_tag="IWO35_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316188.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            1651..1857
                     /locus_tag="IWO35_001119"
     CDS             1651..1857
                     /locus_tag="IWO35_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316189.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            2053..2313
                     /locus_tag="IWO35_001120"
     CDS             2053..2313
                     /locus_tag="IWO35_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316190.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSVFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            2918..3751
                     /locus_tag="IWO35_001121"
     CDS             2918..3751
                     /locus_tag="IWO35_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HAQ0316191.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFETLSDEEKDIYSSLNDNPSFVLSEEIRLLKIRQFRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKNPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            3744..5159
                     /locus_tag="IWO35_001122"
     CDS             3744..5159
                     /locus_tag="IWO35_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase B"
                     /protein_id="HAQ0316192.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            5171..6700
                     /locus_tag="IWO35_001123"
     CDS             5171..6700
                     /locus_tag="IWO35_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0316193.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            6792..7670
                     /locus_tag="IWO35_001124"
     CDS             6792..7670
                     /locus_tag="IWO35_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAQ0316194.1"
                     /translation="MMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            7672..7989
                     /locus_tag="IWO35_001125"
     CDS             7672..7989
                     /locus_tag="IWO35_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316195.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            8038..8739
                     /locus_tag="IWO35_001126"
     CDS             8038..8739
                     /locus_tag="IWO35_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAQ0316196.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMSMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            8754..9644
                     /locus_tag="IWO35_001127"
     CDS             8754..9644
                     /locus_tag="IWO35_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAQ0316197.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLFGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            9667..9882
                     /locus_tag="IWO35_001128"
     CDS             9667..9882
                     /locus_tag="IWO35_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316198.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            9894..10235
                     /locus_tag="IWO35_001129"
     CDS             9894..10235
                     /locus_tag="IWO35_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316199.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            10235..10606
                     /locus_tag="IWO35_001130"
     CDS             10235..10606
                     /locus_tag="IWO35_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316200.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            10599..10994
                     /locus_tag="IWO35_001131"
     CDS             10599..10994
                     /locus_tag="IWO35_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ0316201.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            10996..11373
                     /locus_tag="IWO35_001132"
     CDS             10996..11373
                     /locus_tag="IWO35_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316202.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            11374..11982
                     /locus_tag="IWO35_001133"
     CDS             11374..11982
                     /locus_tag="IWO35_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0316203.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            11982..12323
                     /locus_tag="IWO35_001134"
     CDS             11982..12323
                     /locus_tag="IWO35_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316204.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            12565..15354
                     /locus_tag="IWO35_001135"
     CDS             12565..15354
                     /locus_tag="IWO35_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAQ0316205.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            15344..16084
                     /locus_tag="IWO35_001136"
     CDS             15344..16084
                     /locus_tag="IWO35_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0316206.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            16081..18843
                     /locus_tag="IWO35_001137"
     CDS             16081..18843
                     /locus_tag="IWO35_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ0316207.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            18856..19764
                     /locus_tag="IWO35_001138"
     CDS             18856..19764
                     /locus_tag="IWO35_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAQ0316208.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            19764..20384
                     /locus_tag="IWO35_001139"
     CDS             19764..20384
                     /locus_tag="IWO35_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316209.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            20388..20837
                     /locus_tag="IWO35_001140"
     CDS             20388..20837
                     /locus_tag="IWO35_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316210.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            20837..21331
                     /locus_tag="IWO35_001141"
     CDS             20837..21331
                     /locus_tag="IWO35_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316211.1"
                     /translation="MADIVQLKEDGVAKYLKTHVKAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            21345..21692
                     /locus_tag="IWO35_001142"
     CDS             21345..21692
                     /locus_tag="IWO35_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316212.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKSGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
     gene            21685..21825
                     /locus_tag="IWO35_001143"
     CDS             21685..21825
                     /locus_tag="IWO35_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ0316213.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            21925..22314
                     /locus_tag="IWO35_001144"
     CDS             21925..22314
                     /locus_tag="IWO35_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ0316214.1"
                     /translation="MTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTGLAKAWKLKEI
                     SSEIGFEGLLKKVLIFVVIAVAVDVQKIVGNSIPLREIVIMFYVANEGISFLENISVF
                     LPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            22311..23321
                     /locus_tag="IWO35_001145"
     CDS             22311..23321
                     /locus_tag="IWO35_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0316215.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYSINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTL
                     TDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPD
                     VNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIP
                     KR"
     gene            23511..23584
                     /locus_tag="IWO35_001146"
     tRNA            23511..23584
                     /locus_tag="IWO35_001146"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23545..23547,aa:Ile,seq:cat)
BASE COUNT         8698 a         3444 c         5000 g         7155 t
ORIGIN      
        1 caataaatcc aacacattta ttatatcaca taaaggagcg gtttgacttg atgcaattgt
       61 tacgagaggt agatttcaaa cagacaagat gtaatgcgag agatgtgctg aagaactttc
      121 ggcgtttgga gcggatggca ggtcgctctt tgatagatat taagtcgccg attataacgg
      181 atatgccgaa ggcaccgaag catggcaata aggcagaaga cgcgattatt cagatgatgg
      241 atatagaaac ggagagagat gcgattctag cggctttgat ggctcttagt ctgattagtc
      301 gtcagatact ctactacagc ttctgtgtgc cagatagctt ctcaaactac agaattagcc
      361 gtgaagtggg ttattcagaa agaagtatac aacggatgaa gtcggaagct ctaatagagt
      421 ttgcagaagc atataaacac ggaaaaataa ttgcttataa ataatttggc ggttttttgg
      481 cggaatgatg gcggttttta gacatttacc agtgttatta tggtagtgtc gaaagattag
      541 gaaacaggat cgacaaaata aacgaaggga ggcaatctcc ctcatcgttt taaacttctt
      601 cttagataga cagcaactaa ttatgaagaa aaggatgtga attcaactcc tcctaaattc
      661 ttcatcggtt gctgtttatt ttgattagac agcagcactc acaaaactaa acatgttctt
      721 gtcgtctttc aactgctgct gtctattaat ttatgtattg gagggaagaa aaatgaaatt
      781 atcagtagaa ggcactccag aagaaataca agaattgctc caagctattg gtagtagcaa
      841 ggagcaaaag gtaagtgttt ctaattcaga tattgacggt atttgcaatt gtttaaacag
      901 agaaaaaata tctaatcctt actaagagaa aatatgttcg ttaatgaatt tccaattttc
      961 ctctgtagct ctaccttgat aatttttttg cagttcacag atgaataagg aatcgttatt
     1021 atcaagtact gtatttaatt tttcacacat ttgatttgga ttcaaattag ttttaactaa
     1081 ccaaaaagat ttttgtagct ttatatatgc cccaaattct tttattattt caaatacttt
     1141 atcatacttt tgccctgggt tattaagatc ataactaatc atgtagggtt tatacatgtt
     1201 gtcttgcaca tccttgtttt atatttcagc ggaccactcg ctgataaata aaattatacg
     1261 cttagtattt attttcacaa tattaattta tcgtaataac ttttgagtaa atagaacaaa
     1321 aaacctgcac tagtttccgc tagtacaggc agtgactata tcggttgata atctagcata
     1381 tattaaaaat aattgcaaga aaagaaattt atagtggttt ataccaaatt atcaaagcaa
     1441 tgttattttg ttgctgtcta ttgtttttta attattcaca cgataactaa aggtgggtga
     1501 agagaaatga ttccattaat aatttcaatt tttgcgctct gtcttaatgt ctatatgatt
     1561 ggatttaaaa atgggcaaaa taaaaaatag tagcagccaa gaataatttt atagtgtcac
     1621 tgtggcggaa agggtagacg ctaagcatgt gtgctaggtc aatgcttcgg caaccatgca
     1681 aggttcgatt ccttgccagc gacattaaat gcctatgacg gttacgacta ccgaaaaaag
     1741 atcgttaaga agctatacgg tgctacgtac ggcaatgtag taagtgtgct atctgtacac
     1801 caccaagctt cggtcactgt ggcggaagta gaagacgcag cggtaaatgg cgagtagcct
     1861 cgtgagagcc tggtaagttc tcgtgagtgg tgcaatccca ctccagcgac tttaagcaac
     1921 cgaggcatcg gcggtttaaa aatatagggg tgcgcaattt cgtacgcgtt ttgtgcatcg
     1981 tgcaagttac tattacatat tagatcactc gttgagtggt ctttttattt tagaaaagag
     2041 aggattttga aaatgaatca tgagaagttt atcgaaaaat gcaaggctat tgtacgtgaa
     2101 agaattgaga atgagattgc tgacctaagt ggagcagtac ctgagtttag tgttttcata
     2161 gtctggtcat gcaaaacact acagaatagc aaagcattag tcagcgctag cttaaaagga
     2221 gcaccgtatt ttgaaattac gttgaacggg gacaaaggtg aaatctatgt agatacttat
     2281 ctcaaaaaat caaatgaatg tatcaaagtc tagcaggtgc taggcttttt ctttacataa
     2341 aggaggaatg atctcatgaa acaatatacc gctaaagatt tcgaggaaat gaagcgatta
     2401 aagaaggact atgaagaagt tgatatggag ctaactgttg gagtcattca acgaagactg
     2461 cgggtcggat tagagacagc aaaggctatt tacaatgatc taaatgctat tgaagagaag
     2521 aatggctaat gaggaactac tggtatatat cgctaactaa tgaatatcct cgaaccattg
     2581 atgattgttc agtgcgtgtt gtgcgttctg tacaaatcaa agggaagtac tctattgtcg
     2641 aaatggggag agaagcagaa ccatgtgaga ttgatgcgtg caagctcgtt tattgcggtc
     2701 atggtttcta tgatgaacca aacattcaaa ataatattaa caagaatttg agggattaga
     2761 atgcaagaaa tggcttaaaa atctccaatc aggacaaatc aagttagaca aagtttccga
     2821 tttaaagata ttaattgaag cagatctaat gttgaaagat attgaaaatt agaaaacaaa
     2881 actcaaccta agaagattgc gaggtggtgt gtattgaatg gcaagacaac gtgatccaag
     2941 acgtgatgaa gccaaaagaa tttggttaga atccaacgga gaaaagcagt taaaggaaat
     3001 tgcatctgaa ttaaatgttt cagattctca ggttagaaaa tggaaatcgc aagacaaatg
     3061 gagcgctgaa ttgaaaagta acgttaccaa tggcaaaagt aacgttacta atcaaggtgg
     3121 cgctcctatt ggtaatcaaa atgctaaagg taacaaagga aatagccgag cttctccgcc
     3181 agtgggtaat aaaaacgctt tgaaaacagg cgagtatgaa accatatttt ttgagacact
     3241 aagtgatgaa gagaaggaca tctattctag tctgaatgat aatccttctt ttgttttgtc
     3301 tgaagaaata cgtctactta agataagaca atttcgtatg atgaagagaa tccaacaagc
     3361 tgaagctgga ctaaatgatg aagaagtcga acgattgcag cagctaagaa agattaaaaa
     3421 tccgattgaa aaaaatggta aaaagctaga aatcaagcgt gaggttatgc aagatgtgca
     3481 gattagcaga aaaaaacatc gcaaaattga tgatattctt tcaattgaag attcattgac
     3541 tcggattagc aaccagttag ctaaagccat caagcaaatg aatgaacttt atatgaatga
     3601 atacagaact gatttaatta aagctcagac tgataagatc caagctgaga caaatgaaat
     3661 tggcggaaat aattcaggtg aagaaataga agaatggaaa caggcagttt taaatgccgc
     3721 aaacaaacgg gcggtgaaag aaaatgaata atgaatttat tccttttgct gatattggtt
     3781 ctgccattga ttattactat gataaaccag tagctttttg ccaagatatt ttgcatttga
     3841 atcctgatga atggcaagaa aatgttttaa atgatttagc tgaattttca aaggtttctg
     3901 ttcgttctgg tcaaggagtt ggaaaaacag cattagaagc aggagcaata ctttggttct
     3961 taacgtgtcg accctacgct aaagtaatag caacagctcc gacaatgaag caactttacg
     4021 atgtactttg ggcagaggta gctaaatggt taaatgatag cttgatcaaa aacttactga
     4081 agtggacaaa gaccaaaatt tatatggttg gtgattcaga gcgttggttt gctacggcta
     4141 gaacagcgac taaaccagaa aatatgcaag gttttcacga ggaccatatg ttgattgtgg
     4201 tagatgaagc ttctggtgtg tctgatccaa ttatggaagc tattcttggt acgctatcag
     4261 gttttgataa taagctgttg atgtgtggaa accccaataa tattgaaggt gttttttacg
     4321 attcccacaa ttcagaccgt gataaataca gagttcataa agtatcaagc tatgatagta
     4381 aacgtacaaa caaagacaat atagaaatga ttcttaaaaa atatggaaaa gaaagtgatg
     4441 ttgctcgtgt ccgtattttt ggagaatttc ccaaaggtgc gttggattca tttatcagtc
     4501 ttgaaacggt tgaattggct acagaaaaac aaattagtga ttctttagtc aataaaacaa
     4561 cggttgctca tattggtgtt gacgtagctc gatatggtga tgattctacg attctctttc
     4621 ctagaattgc taccagggca ttggagtatg agaagtattc aaaacgtagc accatggaaa
     4681 caacaggata tgtcatcaac atggccaaga atctaatgag tcaatatccg agtattgata
     4741 aagtgatgat taaagtcgat gacactggtg tcggaggtgg tgtaaccgac cgcctagaag
     4801 aacttataga agacaaacat tatccttttg aggtgtttgg agtgaataac ggttcaacat
     4861 cagaagacga tttttacgat aatttaggta ctcaactatg gggaaacatc aaggaaatgt
     4921 tagaagaaaa tatgacagca aatcttaacg gagaacagcc tgttattgaa ttgccttctg
     4981 atagttcgtt aatcaaagaa ttaagtactc gcaaattcaa aatgacaagt agaagtcgta
     5041 tacgtttaga aagtaaagat gatatgaaaa agcgaaatat tggtagtccc gatattgctg
     5101 acgcactggc tttagcgttt tatgagccac caagtcacta tcaatttatt caattttagg
     5161 aggtgagctt ttgattagta cagtattatc tttagagaga tataagaagc tacgtgttaa
     5221 atatgcaaca caaattgaag atggaatgtt tgatccgaat ggttttatag aagatatgaa
     5281 accatttttt gctgatcgcg aaagaaaata tctagcttat actagcgaaa agaatgaaat
     5341 agataataga ccaaagccta acactgatat tgtaaaagtt aataataaac ttcatgctgg
     5401 tatgtattca attgtcgtag accaagcagt caatcatttt actggtatac ctatcaaatg
     5461 ggattacgat gtatcggaac aaaaaagaac actcattcaa agattaaaag ataaattctt
     5521 aaaaaatgat atagaacttc cgacagttcc agaagctttt aacaaattaa caagcaatct
     5581 tgattccatg agattcgcaa tgcttgattc tgaaactgca acttttcaag gagcttgtgg
     5641 agtagctttc cgattgttag agcctgtgga agaagatgat ggttggaagt taagagcaag
     5701 taatattgaa ccttggagag cagaaagata tggaaatgcc ggaatctata tcaaagaaaa
     5761 gtatgactct taccagaaaa aattttttca agaaatgaaa gttatgacaa gaaataaaat
     5821 tctgacatat gcttgctatg gtgatttgaa ttttgttaca agcggaacgt tcaaaaaaat
     5881 tgatgagacc gataaccctt tggggacgat taccttggca gaatttaaaa ataatacgaa
     5941 tcgttattgt gattttgaag tagctgagga aattggagat gcaattgatc gtgctttgtc
     6001 tgatcaacaa aacgaaatcg aacaatttaa gcttgcgtac atgcttgtta ctggcacaac
     6061 aatgagcaaa ggcactgcta aagaaatgat gaatcagcta gggattatca acttaaaaga
     6121 ccctactgca aaagctgaat atgtcacaaa aaatttagca aaagatttca atgaatatca
     6181 tatggatcta ttgaaaaaac agttttacac catttgtaaa gcaatcgatt ttaacgatga
     6241 ggtgtttaaa tcaaacagtt ctggagaagc tcgcaagtgg caaatcatta gcctagaagc
     6301 gaaaacaaat actaaagagc aatattttag agaaggattg aaagaatgcg cagaaacaat
     6361 tgctgctttt cttaagtttc atgacaaagt agaaattgag cctgaaaaaa ttatttttac
     6421 tttctctaga tctttgccaa ctgatttaag ttatttagct gaagctttac ctaaacttgc
     6481 accatacgta tccaaacgaa ctattcagag ccaaattcca tttgtaacag atgttgatta
     6541 tgaaaatgaa atgatggaat tggaaagtgg aagtgcttat ccagatagtg aatacaattt
     6601 tggcggaggt ggcaatagtg acgatagaaa cgaagtattg gaccaaacgt cgagaactgg
     6661 aggatcaagc aaggctcaaa caagaaaatc agacacttaa aaaattaact agtgtatttc
     6721 ctgaagcact gaaagagata caagcaaaac tattatcaca agctgactta cacaatatca
     6781 cttatccaga aatgatggag ttttatagta caagtaatca gaaaaaatat cgtgaatatg
     6841 tggaaaaaaa ttataagtca ttaaaaatgt atgatgcaaa atacaaagag tttatcgatg
     6901 aattttttcc accatttgac tatgcaaaag tcaatcgctt attacaaata cgatcagatg
     6961 tatttaaaat tcttgcagaa tatgcgatgg atgcagatgt gaatcaatat ttttctgatc
     7021 gcttagagga aattcttcaa agaacatatt cttctaatgc taatgttttt gttcaacttt
     7081 taaacgttga tataccaaat tatttaccag aaaatgaaat taaacattat ttgaactatc
     7141 catggtcagg aaagacattt tcaagaagac tttggggaaa tatttcatcg cttgaacaga
     7201 aactctctaa tgctattgta aaaagtgttg ctagtggaga aggcgttata cacgcattaa
     7261 acaccatgag attagactca gaaatttgtg acatgtttaa gttagaagaa tcaaagtata
     7321 acaaagcgat agaaaatctc gttcgaacgg agtatgcaaa atttgcacaa gatggtattg
     7381 aaaaatcata tttagaaaca ggtattgagg aatacaacgt attgactgca aaagatgaga
     7441 gagtttgccg gatttgtgga ggaaaggcaa gtaagaatcc ctataaactg aaagatgctg
     7501 tcataggtga aaatcgagca cctttccata gtcgttgtag atgtacggat gttcctaatt
     7561 taccaaaatt aggaaaggat attgatgaag aatatgaccg tttatttggc gatttattag
     7621 atgagtttgc acatgattct tttggaatta atttgaaacg gaggaagtag aatgaaagat
     7681 ttttttgaag cagtactaac aattaatgta aatgctgata ttgcagaagc ctacaaaaca
     7741 gctattgagt ctgagaacca tcctaatggc ttgagagacc attggaatgg caattatgcc
     7801 tacgtggtta ttggcgatca aactgttaat tatcaagata atactccagt tgataagaat
     7861 accgttaatt taacgattca attattatct cattcattac caaatttaaa agaaacagtt
     7921 gattggtatg aaaagatggg atgtattgtt gttagaactg actacaaaga aggaaagtct
     7981 agtaattagg cttttttatt ttgtccgaaa tgacgttaaa ctagcgcaat actgggcttg
     8041 gttgaatggt ggggcgcaac tattaaaact caaagcaatg cggggcgtga aaacgaatcg
     8101 tggggcgaaa ggagaatgag catgaaacac aaatcattaa tgccaatgaa tttgcaatac
     8161 tttgccgaag gtgacgatca aaagttttct tttgatgact tcaaatcttt tgtggaatcc
     8221 aatgaagaag ctcaaaaatt tattcagtca caatctcaaa gtgttgccga caaacaattg
     8281 gaagcttgga aacaaaataa cttagataaa attaaacagg ataccatcaa ggaatatgag
     8341 gaatctaaga aaaacaagtc acctgagcaa attcaactgg aaaaactaca agctgaattt
     8401 gaagcagaaa aagcgttgcg tgtgacaagt gataataaag catttgttgc agaacaaatt
     8461 gctggattag aactagatgg agagttaaaa gagtctattt ctcaatttat gctaaataat
     8521 cttgttagtt cggatacaga tttcactaag aatgctgttg aaggttttac aagtgtcttg
     8581 aatgcaatta atgagaaaca tgcagatgca ttaaaagaac tacaaatgaa gtctgcattt
     8641 ggtggaactc aacaatcgaa taaccaggtt cagcagaaca atgaaacatt aacaaatcca
     8701 gaagaacaat taggacaaat tcttcaacaa tttaactagg agagtgaaaa attatgaaaa
     8761 aaacatcttt aaataattta gagtatttgg atatttcacc agcgattaat gctatgcaag
     8821 taccaaatac acctttttta agctatttat ttggtgctgg aaaaacagag ccagcaaact
     8881 cgacagaaat taaatggcgt gaatatgata tcaacaacga tgattcttct gaaaaacttg
     8941 agggcggaga atatccagat gctgaatcag gtcgaacttg gtttaacaac tatactgaaa
     9001 tttttagaaa atcaacctct gtatctggta cattagatgc tattaatgtg aatggagtcg
     9061 gaaatgaatt aactaatcaa gtagcactac gtggtatgga aatgaaaatt gatttgaacc
     9121 gaaaattgat tactggtgta aaagctgatg aaaatggttc taaaggtcgt cgaatgaatg
     9181 ggattttgaa cttgatcaat tcagcaaata aggcagaaac agccactgcg ggtgcagtaa
     9241 caagaaaaga tatcgatact ttatttaaat tgatgtatga aaaaggttat atgggagaaa
     9301 aactatgctt gatttctcca gatatgcagg agttaatgac tgatgagtta gatggaaaat
     9361 caacaaaaat tgttcagttc ggggaaagag taacttttgg attgcaaatt ggaaatatcg
     9421 tgtctaatta cggtacaggt attgctctac tagaaccatc attgccaaaa ggaacaattg
     9481 ccgcaatcga tactaattat gtgaaactac gtccattacg tgaatggaga gcagaagaac
     9541 ttgcaaaaac aacggattct agacgtatcg gtcttgtagg tgaatactct cttgaataca
     9601 atgcttcaaa ctctggggca attttaaatt taaagtctga ataaaaggga gttagtactc
     9661 ccttttttga taggaggaat tacagtggta aaaaaagatg aaactaaaaa agatgaagtc
     9721 gtgaaatata gagtaggtaa aactaaaaat tttgttgggt ttgttcatcc taaaactcgt
     9781 agatttatca cagcagattc aaataacgaa tttatcattt ctatagatga taaagaagca
     9841 attgcaattt tggaagatgc aattgatgtt aatgaaattt aggaagtgat ctgatggatg
     9901 aatcgctaaa aacggaaatc attgagtcta caaaagaaga ttttccagat ttgagtgaag
     9961 aacgcataac taatttatta gaaataattt tgctagaaat tgaatcatac aacacttgta
    10021 aaaatgatat ttcatgggaa aagttaaaaa gcgtgattag tgaagtgttg tataaaataa
    10081 taaaaaatga atcagaaaaa acagtatctt cagttagacg tggcgataca acgattagtt
    10141 atgcttcaac aacaaatgat gttagtgagt tacttctagg ctatggtgat ttgatacgaa
    10201 gagttattgg ttgtggagga ttggagtttt tttaatgaat gaagcagata ttttagaaac
    10261 tacttatgaa gatagttgta ttattgaaag actgacggac attgaagatt ctaatacaaa
    10321 tattactatt caagattaca aaaaagtata tgataatcct atttcttgcg ctctttcaca
    10381 aggtcaaatc gatggactag cagtcataga agatggagag atggtaaatg tttcaactga
    10441 cacatataaa ttatttgttc atcctaagat taaactaaaa aaaggagatc gaataacaat
    10501 aactcaaaag gccagtggct taattttttc tctatttgct actaagcctt tttactatcc
    10561 tagtcattgt gaagtgaatt tgataggaag tgagaaaaat gggtaatctc aaatatgaat
    10621 caaatgcaga aaagatcatt gagaatttta aaaatatgac tgtaattgct caaaaagaag
    10681 gaatatcctt cgttaacgat tcaatgaata aagtcgttag tttagctaaa ccattaactc
    10741 ctgtaaaatc aggtaattta aggcgaggat atcgtgtagt aaaagctaga aaactatcaa
    10801 gcggtcgaat tgttggagca gttataaaca atgaacatta ttttaaatat gtagaagaag
    10861 gtcataggac taaaaatggt ggctttgtca aagggaaatt catgttaact cgtgcaacga
    10921 atcttgcaaa tatgtcttat attcctcgaa gatttaaaca aatggcaata aaaatcgtta
    10981 agaaaggaaa gtaacatgaa agatgaaatc attgctgcaa tcagtagcaa attaaaagaa
    11041 atctatcctg atgggacaat atatcttgat tcagttatgc agtcaactaa agatttttat
    11101 ttcgttttat cagtaatgga atctggaact gaaaatgtag gaattgatat tcaaaatgtt
    11161 tctttcttaa ttgatattgc attgattgat aataaaccta atagaaattt aattaatgaa
    11221 ttagtctcac gttgtgggac tttttttaat acgattacaa tcgatgagca aatattattt
    11281 ccaaaagcct atttacctga tgaagcagat ggtgttcaac atattcgttt tacattagaa
    11341 tttccacaat atattgaatg gagtgaaaga taaatgggag aaaaaagaag taaaaccgga
    11401 attatttctg tcgaaaaacc tacttggttt cctttggaag atgagacagg gaattttccg
    11461 gtatatggta ctgcgttgcc aatgggaaca gcagtaagta tcaaaccaac agctaattat
    11521 gaaacaacac aagattatgg ggattcagtg gttcaagatc aatttacagc ttttggtggt
    11581 gcagaagttg aactggaagc aaatggatat actcataaag ttttatctac aattactgga
    11641 gggaaaattg ttaaaggtgg tgccttgcgt tctggagaag atattgcgca agatggtgcg
    11701 tttgcatata gacgtaaaaa atcgaatggg aaataccgtt atacggtctt ttataaagga
    11761 cagtttgctc tagattcaga tgaaacatca acgattgaag gaagtaaagt aagttttact
    11821 catccaactt ggaagggttc atttgtagat gtaccaggac ttggatatat gtattcagtt
    11881 gatgaagatg atgaaggcgt ggataaagca atgatcggaa attggtttac aaaagtagca
    11941 attccaattg aagaagcaga actttcagga ggtacagaat aatgtcaaaa tatcaaacaa
    12001 cgattaaatt aacgaaaaaa gatgaagaag gtaaatatga gcaagtacaa tttaaatctg
    12061 ctgaattttt accaggaaca gttgtagaag atgctgcagg agttatggaa gaaatgcaaa
    12121 cagcgactga taaacaatca gttaaaaaag ctttaagtcg tgcttattca tttattgcag
    12181 atactttatt tgagggacaa tttactggtg aagattattg caaaggaatt gatgctcgtg
    12241 agattgcctc attgacagga aaattattga agtctgttac tgcaggtttt gatgaaactt
    12301 atacagagac gaaaaaaaag taagtgaggc gctcaagtca ccttcattta agtattcgat
    12361 tacttaccga gaattagata taaagacgca attactcgaa gcaggttgga cgttaccaga
    12421 aattgaacgt actgacttgg atgagttaat gcgtcttttt gcttttagag atgcggtaaa
    12481 agagcatgaa gatgttgagt actatgataa tttcactcaa ttttaggagg tgatactttg
    12541 aataacgatg acctaattct gaagatgata ctagatgaat ctggttttac tgccggtatg
    12601 aataatgctg tcaaaaagtt gaactctttt gatgaaacaa tcgaaagaaa aagtagaaat
    12661 agtggcagtt cattaggtag tatttggaaa atatttgctg gaagttttct agctagcgga
    12721 gtaactagaa tcgtaggagc tggctttgat ctaatcaagg gttccataag tggagcggtt
    12781 gatcgagtag atacgatgaa taacgctcta cgaaatttcc aaaatatggg atttagtaat
    12841 tcagaaatta tgaagaatat cggaaagaat gggcttttat ctcaaggtat tcaaggactt
    12901 cctactgctt tgaatgatgc gataagtcat gttcaacttc ttgcttcttc tacaggtgat
    12961 ttaactcgtt caactcaaat atttaaagct ttaaatgatg gaattcttgg tttcggtggt
    13021 tcaactgatc aagttaatga agctgttatt caattatctc aaagtttctc aaacggaaaa
    13081 gtagacgcac aaacttggaa ctcaatgatc aacgctcaac ttggacctac tctttctgct
    13141 attgctaaaa agatgggaat tacaatggga gatctgaaag aaggtttgtc tcaaggtaag
    13201 atttctgttg aagaattcca aaatcaatta atagaaatgg ataccaaagg tggcggagga
    13261 cttaaatcat taagtcaaat cgctaaagat tctactaagg gaattaaaac ctctatacaa
    13321 aatgcaaaaa cagctgttac acgtggtgtt ggtgaggtaa tagaaggatt aaataaagca
    13381 ttagtagatt cagatttagg tggatttaag ggaattattg ataaagtggg cagttcaatg
    13441 gaatcgttct taaaagtaat tgctgcaaat attcctacag cagtatcatt tttaagtaat
    13501 ttatttaacg aaattcaaaa atttggttct gctttgaaat tcatgatgcc ctttcttgtt
    13561 cctgcagcta ccgcttttgg agcgttcatg tttcaactta aaggtatacc agcaataata
    13621 aaaagcttca ataattttaa gaatgccata attggcgttg gaaattcatt aaagattatg
    13681 ggggcaatag ctgctgctaa ttcatttgtt ttaattattg gtgcagtcgt tggtgcaata
    13741 gctgttttcg gttactttat ggcaaccaat gaagagttta gaaacaaggt tatatctatt
    13801 tggaatgatg tgaaagattc cgtaattggt gtattaaaaa atataaagga ctggggaatt
    13861 gatacttgga attctgctaa agaaatggca tcaaatgcag ttgagggtgt caaagatgct
    13921 tggtcaggaa caaaggaatg gttttctaac acctggaaag gtattaagga tggggccaca
    13981 ggtttatttg ataaaacagt agagacttca agaaatgcag tcgatagtgt aaaaaatgca
    14041 tggtctaata tgaagaaatg gttttctgat acttggcaaa gtataaaaga ttcagccaga
    14101 gaaaaatgga atgaaattaa aggttctatt atgtcggttg ctggtccatt gattacaggt
    14161 ataaagaatg catttttaca tgttactttt tatctagaga ccttatggaa taatttagtt
    14221 gagattggta aaaacgtctt tgagatttta aaaaatgtta tacttgcgcc ggttttgttt
    14281 attacctcca tgatttctgg tggatgggaa gaaacaaaaa ataatatgat tgctgtttgg
    14341 aataatatta aagaaagtgc cataaatatt tgggaatcta taaagaatat atttgtaagt
    14401 tattttacaa atatttattt tgctgcactc aatatttgga cagggttcaa gcttacattg
    14461 ataaatattt ggaatgaagt ggtaaatcaa gctaaatcga tttggttaaa cataaagtat
    14521 ttttttatta atctttggat tgatataaaa tattttgcaa ttcagaaatg gattgaattg
    14581 aagttttcta ttattcaaac ttggatcgat ttaaaatata atgccattac tacttggaat
    14641 aatattaaac agtttttcaa agatacttgg aaaaatatta aagatacggc atacaatacg
    14701 tggatttcta taaaaaatac catgattaat acatggaata acattaagga ctctttctgg
    14761 aatattgtta ctggaattgt taattctgct gaaaatgcat ggacaaatct aaaaaatggt
    14821 gtttcaaatg ctattaatcg ggtaaaagaa atctttaatt cgttaaggga agtcaattta
    14881 tttgaaattg gcaagaacat cattgatgga cttataaatg gtgtaaaaga aaagtggaat
    14941 gctttgaaag atactatcaa aggtattgca ggtaatatca aaaattggat taaaggagct
    15001 ttagatattc attctccatc aagatggatg cgtgatatgg ttggtaaaaa tattgttcaa
    15061 ggtattatcg ttggtattga taaagagcaa agtaagttag atcaaaccat gacaaattta
    15121 gtaaaaacac cgtctgttca acctgtagta aatggaataa gtcacctacc aattacacaa
    15181 acaaagcaaa atatatctgc tagtgaaaca aaagaaattc atttacattt aaatgtttat
    15241 ggagatttac ctgattcaat ggttcggaag attaccacaa aaattaaaac agaattgaca
    15301 agacaaatga aacgagatgc tggtgcagta ggaggaacat tatatgcaac ttaagagagg
    15361 acaatttttt attaatcaac attattcttc tgaatttaat gtgtatattc aaaacaggcc
    15421 tgcttctgtt tcagctagtc gagtaattga attgagagag cgagaaggaa atgattctat
    15481 cattattgat aaaggctatt acagaaatgt gactagaaag attgagtgtt actataaagc
    15541 accttcaatt gatgtggtgc aggagtggga agatcgaatc actgaatggt tagatatgtc
    15601 gtcttatagt gattttattc tttactatga tgaacaatat atttatcaag ctgtagtaat
    15661 agaaggacct gaatttaaag gaacaagaaa aacaggaaat attgtccctt ttgaatttac
    15721 agtgagcatt cgaccattta aggaaaatta caatggtaga tttactattc gacaaacaga
    15781 aacttttgaa atttataacc cagagaagta ttcttcaaaa ccgcttatta aattgagtgg
    15841 ttctggagat gcttcttttt atattaataa ggacaaatat gatttgaaat cgttggacag
    15901 agaattattt atagattcta aattagaaga ggcttaccga aaattagatg gtaatttaga
    15961 acatcaggat caagtcactt tatttttaga ttttccattt ttatatcctg gaaaaaatga
    16021 aattaaatgg acgaaaaata ttcattcatt tgaaataatg cctaggtggt ggagaaaagt
    16081 atgaaaccaa gaatatatag tcctactgaa acagatttct caacgaatgg cttaggaatt
    16141 ttaaaggatt gtacaagatg tgaaatatat gaagtagcta atggaaaata tgaattagag
    16201 ttggattatc ctttaggaac tagatttgat gaatattttg aaaatgacta tcaaataaaa
    16261 gcaaagccaa acgatcaaga agagtatcat atctttttta ttgatgataa agatatagat
    16321 acttttttaa atacagtaac tatttatgct cagagtcgta caaatcgact tggaagacgg
    16381 gtagtcactc ttgcggaagt agactctaaa actggtcaag aagcgatatc aattattgaa
    16441 accaaaatgg ataaaaaatc tgacatacga ctttattctg atattacggc cgtttctagc
    16501 acaatctttg aagcgagaaa tgttttaaat tgtattgctg gtgaacaagg atcattgctt
    16561 caatattggg gtggagaaat taaacgtgaa ccatttaagc tttctttgtt aaagcgaaga
    16621 ggtcgcaata atgttggaac gattcggtat ggaaaagata tgtctggttt aaaggtcaaa
    16681 ttagattgga caggtataaa aacaagactt attccatatg ctgatcctca aagtgaggta
    16741 ggtacgacta atcgaattta tggttcgcca gtagatagtg cctatattaa taactatcct
    16801 gatgtgtata cagagcatgt tcagtttaca gaagaacaag gagtaaaaga cattaagagc
    16861 ttaaataaaa tagctaaaaa ttactttaaa acgatcaatc ctggctgtga taagcctaaa
    16921 gtttctatta cggttgaatt tgataagttg actgatagtg aagaagcgaa agaatttgcg
    16981 aagattagaa attatggtct gtttgataca tttaaaatat atcataaaaa atataatatt
    17041 tatcttgaat caaaggttag tggactacaa tacgattctt tatctgaaaa aactttgaaa
    17101 ttagaagcag gagacattca agttgctttt tatcagcaac aagctgtaac tattcaggat
    17161 aaattaaaag attatgcaac gaataattat atgagtgatt ttaatgatta tgtttcttca
    17221 atgattacag gtcaaggcag tgcaggagga tatgtaagtt tatggccaaa agaaaaacct
    17281 tccaacatct ttattatgga taatccagat ttagaaaagg caagagaagt actaagaatg
    17341 aataaaaatg ggattgcttt ttctaagaaa ggatggcggg ggccttttac ttcagcttgg
    17401 acattagata gtatatttaa tgccaatttt atacaaactg gattaatcaa agcagatatc
    17461 tttcaaaatt cttttaacaa gactggagat gtattgaaat tagttaatgg actactccaa
    17521 atttggaaca acaaaaagaa aataatggag ttaactaaaa aaggaatgga attttggaat
    17581 tctaagggtt caattggaac gattggaaca actgattctg ctggtaatcc ttttcctggg
    17641 gcttctactc caacccctat tgaagataat tctttagtta ttcgtacaaa tggagacggc
    17701 aaatatattt tgatttctcc taaagtagga aaaggattag ttttattagg aaatggtaaa
    17761 gcaatttatt ttggagactt agatgtacaa ggaaaactta cagttaaagg aaaagaaata
    17821 attcctggac aaggtggtgg tccatcggga ggtggagaat ctactggtgg gtatcctagt
    17881 gaattaaaaa cagatgcaga aaagagagct tggagaattt acgatatttt gtgtaataac
    17941 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
    18001 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
    18061 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
    18121 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
    18181 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
    18241 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
    18301 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
    18361 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
    18421 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
    18481 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
    18541 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
    18601 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
    18661 ggacaagtgg gttcatggtt tgcagacgga acttatggac atactggcgt tatccgaggc
    18721 ttagctaatg gtcgtatgca aacttatgaa caaaacacag agttaggtat gatttgtgga
    18781 aagttagatc gccagtatta tagtgcaagt gcaatttctt ccattgtcat accaccaaaa
    18841 tagggaggtg atttaatggc aaagtggaat gtcatattaa gtacaacaga accctataat
    18901 tacgtgggga tgattcaagt tcgacaaggc aataaaaaca ctgaagttat ggaagctaca
    18961 attgtcgaaa atggtcttcc atatgattta tcagaatgta aggtatattt tgaatcagtt
    19021 gttggcggaa aatatccagt ccaattagaa acaagaattg tggatgctaa aaaagggaaa
    19081 atcaaatata tttttgataa atattccatg cagtgtttgc atcgacaaac agccaatttc
    19141 attatattta aaggagaaga cttgattgga acaactcaag acttctccta ttttgtcatc
    19201 aatgctgttt caaaaacaga aggagaaatg ggttcttatt ggcaatcaat tgaagattta
    19261 attgctgata tgacagattt tattaatgaa aataaaggcg attttacgga ctggatgaat
    19321 gaaaggaaag aagaatttga tcgctggaga gaagaacagg agaatagttt ccaagattgg
    19381 agagaaggac aagaatcaga ttatttgtca tggtttgaat caatcaagga tattttgaag
    19441 tctatcgatc caggtggagt aatgttagcc gaattaatgg atgcacgtgt agatcttcaa
    19501 ggtgtacgcc atgaatcgat ttctgaacgt ttcctatccg atctaaatta tttgtatcaa
    19561 aaaatgaaag cagcactttt cacgattgaa tacggtgaaa ttgaagtgac tgatattctt
    19621 caagacgatc tcttttcaga taatcacgaa gtcgaaaaag ttggaactgt agaattcccg
    19681 attgaagaag gagccttgat catcgcaacc gttgatgatc caaagcaaaa tgtgttcact
    19741 cttgagaaag tcggggtgat ctaatggcta aaactaaacg aatgatggaa acggatgaaa
    19801 aaacaggtgt acaacgccaa ttctttccaa tcacacatgc ttccgcagtt cttgggttag
    19861 aagaaataat agcaggagaa gcaacagttt tatctgttaa tggaaaaatt ggagccgtcg
    19921 tcattactaa agaagatttg ggattagaga atgttctcac agaattaccc tatgcaagtg
    19981 aagaagatga cggcattatc acggcagaaa tgtatcaaaa aattttaaac agtggagaag
    20041 gtgactacgt gttgccagtt gccactatcg accgtttggg tggcataaaa gttggtgaat
    20101 tattgacgat tgatgagaca gggaaagtct ctgcagtcag acaatctgat gtcaattttt
    20161 cgctggagtt aaaagaaaaa ctcgattcac tgaaaaatta tactgctgga gaaaacatca
    20221 ctattgatga agatggaaca attagttcta caggtggtag tgggactggt ggtgtcaatc
    20281 agtcatatgt agatcaaaaa ttccaagaag ctgtaaatca agcagaaaat tacacaaacg
    20341 aaagaattcc aaattttact tttgaaaaaa ttggggaggt atagagaatg acagatatcg
    20401 taaaaataaa acaaagcaat gtacaggttt atccacaaac tcattggaat gctatagaag
    20461 gcaaaccaac aacggtgaag ggagataaag gcgatcctgg ccaagcagca acaattacca
    20521 taggaaccgt ttctagtggt tcaaccgctt ctgttacgaa tgtaggaaca tcatctgcag
    20581 ctagatttaa ttttgtttta ccaaagggag ataaaggtga tcctggaata aatgccacaa
    20641 ctacagctgt agctacaact acagcaaatg ggttgatgtc gtccacagat aaaactaagt
    20701 tggatggaat tgcagctgga gcacagaaaa atccaggtaa tgctacaact acgacagcag
    20761 gtttaatgtc agcaaccgat aaagtgaaac ttgatggatt agccaatatt acatttgaga
    20821 aagtggggac ggtttaatgg ctgatattgt tcaattaaaa gaagacggag ttgctaaata
    20881 cttaaaaacg catgtaaaag ctattgatgg taaagaagca ttggtgcaga cagatggaga
    20941 ccaagccatt gcaggacata agaatttttc aggttctgta acaatcaata ataaacgtgt
    21001 tttgacgact gatgataaca gatatgaagt ggtaaatctg gttgtcacta acggtaatac
    21061 agggacagca aagctttatc gtgaaggaaa aacagtttct atatattttg tggctttaaa
    21121 cggaaaaagt agtggcggga atgattcggt tattttaact gttccagaag gctatcggac
    21181 accaattagt tttgaacaac tggttgggtc aatagaccgt tctactttga acagtgctca
    21241 actatctatt ggcgcagacg gagccattaa atggagaaga aattcaagtt atggatcagc
    21301 ttattcattt gttatcactt attcaattta agggaggaaa tctaatgaaa gtagtttaca
    21361 aatcaatcaa gccttacgga ttcgagcaaa tcattttgaa caatcaagaa aatatccctg
    21421 aaaactgtac agagattaaa ccaccagttc ctaactggag accaagattt gattttgata
    21481 aaaaacagtg ggttgagtta gctactgaag aagagaagag tggcacagcg gttgacgata
    21541 ttgaagatgt cgatcagttg aagcaattaa atgctctact gacaaaacaa ttggcgatat
    21601 cggtacagga acaagaaaaa atgcaacaaa tgttagctca attgacgatg gaagtcgcaa
    21661 gtattaagaa tggagggaaa agtaatgaat agttttccgg gctttgaaaa tataaaacaa
    21721 ttttatgatt ggggatgtta tacggaccaa gatttacttg actatgtaaa catgaactgt
    21781 ttaacaaaag accaatataa gcagattact ggaaatgaaa tttaattaag cacaaagtta
    21841 aataaaaagc gtactcaaac gagtgcgctt ctttgcataa aggagaaata gatattggaa
    21901 aaatatttaa atacactctc agtaatgaca ggggtgatag gcgggacaat tgttggttta
    21961 ttgggaggga tggacaatat actacatgtt ttaatttttc ttgtgggtgt ggacttctta
    22021 actggacttg ctaaggcatg gaaattgaaa gaaataagta gtgagatagg tttcgaagga
    22081 ttgttgaaga aagtcttaat cttcgttgtg attgccgttg cggttgacgt acagaaaatc
    22141 gttgggaatt ctatacctct aagagaaata gttattatgt tctatgttgc aaatgaaggg
    22201 attagttttt tagaaaacat ttcggtattc cttcctttgc cagataagtt aaaagaggta
    22261 ttccaacaaa tacgaaatga tactgaaaat aaagaccgag gaggaaccaa atgaaaaaga
    22321 aaatctttgt aggagctatt atagctcttt ttttattgcc aataaacgcc tttgcttact
    22381 cgatcaacaa tgaattcaat ttgggcgcaa acgaaggtag ttctcaagta gcaaataacc
    22441 aatacatcct attgcatgaa acagcaaacg aaactgcgac aggacgaaat gaagcgcagt
    22501 acatgaaacg ttcttggtac aatgcctata cagcgtatat tgttggcgat ggtgggatcg
    22561 tttaccaagt tggacaacct ggttatgtac aatatggcgc tggttcatat gctaatgcaa
    22621 atagtcctgt gcaaatcgaa ctgcaacata cgcacgataa agtaacgttt gaaaagaact
    22681 acaaggcata tgttgaatta gcgagagatt ccgctaataa atacggtatt ccacttacat
    22741 tagacacacc gtataatcaa ccaggaatca aatcacattt atgggtaact caaaacattt
    22801 ggggcgatca cactgatccg tatggatact tatcagaaat gggagttagc aaagaaaagc
    22861 ttgcttatga tttagctcat ggatttaccg atgaaaatcc aacaacttct gaaaacaagc
    22921 ctgtcattga tccaacacga gctggtgcag ctaatcctac gctgacagat ggaacaaatc
    22981 acgcccacat tgatcagttt ggggaaatcg aaaacgcaaa cttgcatgtg gctggatggc
    23041 acattgctaa ctataaatac gagtatattt tcattatgga ctacaatact gggaaagaat
    23101 tagctcgagt aagagctgat ggaatttata gaccagatgt aaaccaagct tataatactt
    23161 ctggaaatgt tggttatcat gtatctttca atatgcgtaa ttttcctagt aagaaagtct
    23221 atgtaatgat gcgggcaacg aatgatccag agggaaacac taaaggcggt gcgcaagatt
    23281 tccatgacaa acgttggtat ttaaatattc ctaaacgata aaaatagctc ctcgttgagg
    23341 agcagtacat aactatattg acaactataa aaattattcg ataaaatagt gatgttatcg
    23401 catatcttca ctatcaccca taaatagtca cactccaagc tatgcgataa caggtttgtt
    23461 gccacacatt ctactggttg attgtttatg gctttatgtg gcaacaacca gtacccttag
    23521 ctcagttggt cagagcagac ggctcataac cgtccggtcg taggttcgag tcctacaggg
    23581 tacattaacg tagccatttg aatcgttctg tgttagaatt tttttgaaga gtattataca
    23641 agctaaagct tttcttcatt gccactcaaa tgagtggctt ttttatgtat ccttttatgg
    23701 attaatgaaa ggatgtttca catagttata cttctgtata tttgaaaagt tttactttga
    23761 tttttaaata gaaagacatt tgggttaaat tgtgagataa taataaagaa gagtttaaag
    23821 cgcaccccaa accacttccc cataagtgtg ttacgcttta aactctttta tatttgaagc
    23881 cattaaaaag cataccatat ttttgaaaaa aagtgagaaa aaaggcttac aattggagtg
    23941 gtagttaatt agtgacttat ttttgatttt atagcactga tactataaaa tatagatatc
    24001 atcatattac acaatcttaa tactaactta aaaaatatct cctttcacaa gtatggtgat
    24061 aaaattcgtt ccgggctacc tttttaggta gcctacttta atctttatac ctttctggat
    24121 caacgaaagt atactttata tagtcataac gccgatgatc gctacgtgcg tccggcacgt
    24181 cagtcatgaa tcggctacac tagactagac agaaaaaata aggtgtgtag aataataaaa
    24241 aacacactgg aggatttttc atgtcaagac gtcaacgaag aacctattca aaagaat
//
LOCUS       DACECZ010000030        23702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.030, whole genome shotgun sequence.
ACCESSION   DACECZ010000030 DACECZ010000000
VERSION     DACECZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.030"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWO35_001147"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWO35_001147"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ0316216.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IWO35_001148"
     CDS             complement(1172..1867)
                     /locus_tag="IWO35_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ0316217.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IWO35_001149"
     CDS             complement(1880..2596)
                     /locus_tag="IWO35_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ0316218.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="IWO35_001150"
     CDS             2926..3534
                     /locus_tag="IWO35_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ0316219.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IWO35_001151"
     CDS             3859..4182
                     /locus_tag="IWO35_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ0316220.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IWO35_001152"
     CDS             4364..5734
                     /locus_tag="IWO35_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ0316221.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="IWO35_001153"
     CDS             complement(5864..7165)
                     /locus_tag="IWO35_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0316222.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="IWO35_001154"
     CDS             complement(7519..9510)
                     /locus_tag="IWO35_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ0316223.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="IWO35_001155"
     CDS             complement(9654..9920)
                     /locus_tag="IWO35_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0316224.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IWO35_001156"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IWO35_001156"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ0316225.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IWO35_001157"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IWO35_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ0316226.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="IWO35_001158"
     CDS             13264..13827
                     /locus_tag="IWO35_001158"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0316227.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="IWO35_001159"
     CDS             complement(14080..14601)
                     /locus_tag="IWO35_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ0316228.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="IWO35_001160"
     CDS             complement(14604..15446)
                     /locus_tag="IWO35_001160"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ0316229.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="IWO35_001161"
     CDS             complement(15439..16164)
                     /locus_tag="IWO35_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ0316230.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="IWO35_001162"
     CDS             16340..16636
                     /locus_tag="IWO35_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316231.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="IWO35_001163"
     CDS             17204..18796
                     /locus_tag="IWO35_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0316232.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="IWO35_001164"
     CDS             18809..19549
                     /locus_tag="IWO35_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0316233.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="IWO35_001165"
     CDS             19717..21066
                     /locus_tag="IWO35_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0316234.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="IWO35_001166"
     CDS             complement(21194..21784)
                     /locus_tag="IWO35_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0316235.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21931..23283
                     /gene="brnQ"
                     /locus_tag="IWO35_001167"
     CDS             21931..23283
                     /gene="brnQ"
                     /locus_tag="IWO35_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0316236.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6796 a         4773 c         4511 g         7622 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagccattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caagaactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aaaccgtaaa ggagttatgt atgacaaaaa aattatcatt taaagactat
    21961 ctttttatag gctccatgct tttcggtcta ttttttggtg ctggaaatct tatctttccc
    22021 gttcatttgg gtcaagaagc aggcgctgct accttttggg cgaatctagg tttcttggtt
    22081 accggtatcg gtcttccctt cttaggggtg attgccattg gtgtatcgaa aagctcaggt
    22141 gtatatgaat tagcaaaacg gataaatcaa acatatgcga tggtttttac catcttgctt
    22201 tacttagtta tcggaccgtt ttttgcttta cctcgccttg caaccacatc tttcgagatt
    22261 ggtttagcac catttatcga tcctagcaga cagacgctat atctagcaat tttcagtatt
    22321 gtattttttg tgctggcttg gtggttttca agaaaaccga caaagatatt agattatgtt
    22381 ggaaagttcc tgaatcctgc atttttggtt ttattaggtg ctttgcttct ccttgctttt
    22441 ttgaatccac taggttctgt aaatcaagcg cctattcagc caaattatca agagcatgct
    22501 tttttcacag ggtttactca aggatataac acattagatg cgcttgctgc tttagcattt
    22561 gggatcatca ttgtcacaac aatccaaaac atgggtgtaa caaaacctgc tgaaatcgct
    22621 aaagacacga tcaaatctgg tgcgatcagc attatattga tgggaatcat ttatactttg
    22681 ctcgcttatt taggtgctat gagcttaggt agctttgctc taagtgaaaa tggcgggatc
    22741 acgctggctc agattgctga tcattattta ggtacttatg gcagtatctt attagcattt
    22801 atcgttatat tagcttgctt gaagaccggt attggtttga ttacagcttt tgcagaaaca
    22861 ttcacagatt tgtttccgca aagaaattac gcattctttg ttgcacttgc tagtatcctt
    22921 ccttgtttag cggcaaacgt aggcttgacg aatatcattc aattttcgtt gcctgttttg
    22981 atgttcatct atccattagc aatgacactg attttactag tgttagttgg tcctttgttt
    23041 aagcagcgta cggctgttta tcgaatgact acttatttta cattgattgc ttcgattttt
    23101 gatgggttga acgcttgccc agaaactatc aaacaaacac ctattgttca aaatctttta
    23161 tatgttgcgg aaagctactt gccgtttttc aaattgggga tggggtggat cgtaccagca
    23221 gttatcggtt ttgttatcgg actgatttgg agtttcgcta agaaagaaga agtagcagat
    23281 tgataaaaga ggttgtgaaa gagacgttca gccttgagca ataaaaagta attttgaaaa
    23341 atggcttctc acatttttgc ataaatttaa cttaataccg agaaactgac ttttgaacac
    23401 cgtttattga gaggtcgtga aaaagccgct tagtttcaag tattaagaaa tattttgtga
    23461 aaatggcttc tcacattttt gcataaattt gacttaatac cgagaaactg gcttttgaac
    23521 accgtttatt gagcgtctat aaactgacct gagtattaga gaaacaaaac ggcaaagtag
    23581 ctccatatac tttgccgttt tgttttgaac taataccgaa gagaagctga tcaaacaaca
    23641 tttcttttat aatatagatt ttagacatat atatgatata aaaatagttt tcgcaaaaaa
    23701 ac
//
LOCUS       DACECZ010000031        23483 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.031, whole genome shotgun sequence.
ACCESSION   DACECZ010000031 DACECZ010000000
VERSION     DACECZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.031"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            100..1761
                     /locus_tag="IWO35_001168"
     CDS             100..1761
                     /locus_tag="IWO35_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0316237.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1831..2178)
                     /locus_tag="IWO35_001169"
     CDS             complement(1831..2178)
                     /locus_tag="IWO35_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ0316238.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2262..3203
                     /locus_tag="IWO35_001170"
     CDS             2262..3203
                     /locus_tag="IWO35_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0316239.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3204..4247
                     /locus_tag="IWO35_001171"
     CDS             3204..4247
                     /locus_tag="IWO35_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0316240.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4253..5323
                     /locus_tag="IWO35_001172"
     CDS             4253..5323
                     /locus_tag="IWO35_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0316241.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5313..6266
                     /locus_tag="IWO35_001173"
     CDS             5313..6266
                     /locus_tag="IWO35_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0316242.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6278..7288
                     /locus_tag="IWO35_001174"
     CDS             6278..7288
                     /locus_tag="IWO35_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0316243.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7573..8394)
                     /locus_tag="IWO35_001175"
     CDS             complement(7573..8394)
                     /locus_tag="IWO35_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0316244.1"
                     /translation="MNRFASKEEEKLYYEHEASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8491..8769)
                     /locus_tag="IWO35_001176"
     CDS             complement(8491..8769)
                     /locus_tag="IWO35_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316245.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9037..11046
                     /gene="metG"
                     /locus_tag="IWO35_001177"
     CDS             9037..11046
                     /gene="metG"
                     /locus_tag="IWO35_001177"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ0316246.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11525..12112)
                     /locus_tag="IWO35_001178"
     CDS             complement(11525..12112)
                     /locus_tag="IWO35_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316247.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12287..13609
                     /locus_tag="IWO35_001179"
     CDS             12287..13609
                     /locus_tag="IWO35_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0316248.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13764..14540
                     /locus_tag="IWO35_001180"
     CDS             13764..14540
                     /locus_tag="IWO35_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0316249.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14533..15102
                     /gene="rnmV"
                     /locus_tag="IWO35_001181"
     CDS             14533..15102
                     /gene="rnmV"
                     /locus_tag="IWO35_001181"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ0316250.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15095..15979
                     /gene="rsmA"
                     /locus_tag="IWO35_001182"
     CDS             15095..15979
                     /gene="rsmA"
                     /locus_tag="IWO35_001182"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ0316251.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16430..17059
                     /locus_tag="IWO35_001183"
     CDS             16430..17059
                     /locus_tag="IWO35_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316252.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17183..18292)
                     /gene="ugpC"
                     /locus_tag="IWO35_001184"
     CDS             complement(17183..18292)
                     /gene="ugpC"
                     /locus_tag="IWO35_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ0316253.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18501..18923
                     /gene="mgsA"
                     /locus_tag="IWO35_001185"
     CDS             18501..18923
                     /gene="mgsA"
                     /locus_tag="IWO35_001185"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ0316254.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19246..19782
                     /locus_tag="IWO35_001186"
     CDS             19246..19782
                     /locus_tag="IWO35_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0316255.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19822..21585)
                     /locus_tag="IWO35_001187"
     CDS             complement(19822..21585)
                     /locus_tag="IWO35_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0316256.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21588..23303)
                     /locus_tag="IWO35_001188"
     CDS             complement(21588..23303)
                     /locus_tag="IWO35_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0316257.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7572 a         4297 c         4776 g         6838 t
ORIGIN      
        1 aaaaaaaagt gttgattttt taataattat tcattaaact cttaaacaac ttcagaaaat
       61 attaattatt ctgaaaattc caataaaggg gtacgtaaaa tgaaaaagaa atttacattt
      121 ggtttggttg cggtatgcgg ccttgtactt gctggatgtt atggcggcag ttcagccaca
      181 agcagtagtt cagcaaaaag cggttcagca gatggaggag gcgtatttaa cttagtcgtg
      241 cctcaagaaa tgcctacagc tgacttatca gttgcaacag atacaatcag ttttacagca
      301 ttgaataacg tttatgaagg aatttaccgt cttgatgaag acagcaaacc acagccggca
      361 ggagctagcg agttggcaga agtcagtgaa gacggattaa cttacaaaat caaattacga
      421 gaagacgcaa aatggtcaaa tggtgatcct gttacagcag cggattacgt ttatggctgg
      481 caacgaacag tagacccagc tacagcttct gaatatgctt acctgtatgc gccagttgaa
      541 aatgcagaag acattacagc tggaaagaaa gataaatcag aactagggat caaagcagtt
      601 ggtgactatg aattagaaat caaattgacg aaacaaacac catatttcca atatctgctt
      661 gctttcccat catttttccc acaaaaacaa tccgtagtgg aagaaaatgg ggatgcctat
      721 gcatctgcaa gtgataaagc tgtttataat ggaccattca cattagaaga ttttgatgga
      781 ccaggaacag atacagaatg gacttataag aaaaatgatc attactggga caaagataca
      841 gtaaaacttt ctgaaatcaa agtcagtgta gtaaaagaat cttctactgc attgaactta
      901 ttcaaagatg gccaagcaga tgacgtgatc ttgtctggtg agttagcaca gcaaaacgca
      961 aacgatccag cttatacttc tgttaaagaa gcgcggacaa gctatatcga attcaatcaa
     1021 cgtgaaaaga attcaccatt caacaatgtg aacctaagaa aagctatttc ttattctatc
     1081 gatcgtgaag ctttagtaaa acaagtacta ggtgacggat cagttgtctc tacaggattg
     1141 atcccatcag atatggtaaa aagtccaaca acgaacgaag actttgcaaa agaagctgga
     1201 aaattagttt cttatgacaa agataaagca aaagaatatt gggaaaaagc gaaaaaagaa
     1261 ttgggcgttg attctttaga atttgatttg atggcttctg atgatgattc atcgaaaaaa
     1321 gtaatcgaat atgttcaaaa ctcaatccaa gaaaacttgg atggcgtaac agtcaaacca
     1381 acacctgttc cattctctgt acgtcttgac cgctcaactt cgggtgattt tgacgcagta
     1441 ctaggcggat gggctgctga ctatgcagac ccaagcagct ttactgattt gtttgtcaca
     1501 ggtaattcat acaaccgtgg tcgctggagc aatgcagaat atgacgaagc agttaaagca
     1561 gcttcatcaa cagacgctgg cgatgaacaa gcccgttggg atgatttcca aaaagcaatc
     1621 aagatcatct ctgatgatat gggtgtcgtt ccggtgtacc aaaaagcaga aggtcaccta
     1681 gtgaatgaaa aaatcaaagg tatcgttcac catgcggcag gcgcttcatg ggattacaaa
     1741 tggacatacg ttgaagaata atcgttaata ccgtgagcat gagaaaaaac gaggctgcgg
     1801 caaatgtcgc aacctcgttc tcatattcac ttatcctaac aaagaaaaaa gcatgaaaat
     1861 agcagaaaga gcaattagta ttccggcaat gatcagccaa aagctgtaca taccgtagct
     1921 ggcagacgag agcgaggaaa attctggttt tttctttcgg ttccttgttc gtgcaagatg
     1981 gaccgatcgc tgaaaatgat cgaagaaacc cgaagaaaag acccataaaa aaccgccgat
     2041 aatcagaaac ggcaatgacc ataagaatag atcattagat aattgaatga gtgtaaagga
     2101 atggttgatg aggtttttta ttaaaataac cagaatcagt agaaaagcta ctgcgtaggg
     2161 gatagatttt tttttcattt gattgctcct ttatttttgg ttcattatct aataatgtag
     2221 cgaaacatct gtcagaagtc aaaagctagg gggaaacatt agtgaatagt tatatcaaat
     2281 atgtattaaa acgtgtcttt ttcatgatca tcacgctttg gttaattgca acaatcacct
     2341 ttttcttgat gcaattgctg ccgggcacgc catatacgaa tcaggaacgt ttgagtcctg
     2401 aaacaatcga gatgctaaat aaacaagtcg gtttggataa accagttatc gtacaatatg
     2461 ggatttatct ttctaatttg cttcaaggag attttggaat ttccttccaa tttaaaaacc
     2521 agccagtcgc acatttgtta gctggaagaa tcggaccatc cttgcagtta gggttacagg
     2581 caattatctt tggaacagtc ctagggacga ttttaggaac gatctctgcc atgaagcaaa
     2641 atacatgggc agatacatct tctacactcg tagcgatatt aggtcgttcc attccaaact
     2701 ttgtatttgc tgttttatta caatatatct ttgcaatcaa attacaagtt ttgccaatcg
     2761 caaaatggga tggctttgtg tacacaatct taccaacaat tgcgttggcc atgtcgccgc
     2821 ttgctgactc tgctcggttt atccgtacgg aaatggtgga agtactgcat agtgattatg
     2881 tggaattagc aaaagcaaaa ggattgagcc gttgggaaat cgcctttaaa catggtcttc
     2941 gaaatagttt gattccattg atgactttat taggaccgtt agctgtagca ttgatgaccg
     3001 gatcactggt ggtagaaaat atctttgcta ttcctggtat cggggaacaa ttcgttaagt
     3061 caatcacaac aaatgattat cctacgatca tggctgtaac gatcctttac tcatttatgc
     3121 tgatttttgt tattttgatc gtggatctat tgtacggtct agttgatcca agaattcgtg
     3181 tttcagaagg gagccgagga taaatggaaa tgataccaaa gcgttatgaa acagttgcag
     3241 atatccctgc ggatgaattt cttccattac aaaaaaatac cgaagaagaa cgtgaacaga
     3301 ttactgcacc ttctttgaat tttatccaag attcatggcg ccgtttaaag aaaaacaaag
     3361 ctgccgttat ttcaatggtt ttactattgg tcatcatttt gatctctgtg atcacaatct
     3421 ttgtttctcc tcatgatcca actgcccaaa acgtggctta tatcaatttg cctcctcgta
     3481 ttccgggaat caatatcgat ggattgaatg gaaaagcaat ggttggtggt gaacttgttg
     3541 ataaatatgc tcaagcaaac gtcccttcaa acgtaaattt ctttttagga acagacggtt
     3601 tgggacgtga tgtattaagc cgcttattta tgggaatgag aatctcgttg ctgatcgcat
     3661 ttattgctgc attactagat gtaacgattg gtgtagctta cgggttggtt tctggtttgt
     3721 taggcggacg tgtcgataac gtgatgcaac gttttttgga aatcttatca ggaattccaa
     3781 acctcgttgt catgattttg atgttggtcg tttttgaacc aggaattttt tcaatcgtgg
     3841 ctgctatggc catcacaaac tggataccaa tggcacgtat cgttcgtgca caaacaatga
     3901 aattaaaaga tcaagaatat gttttagctg gattgacatt aggtgaatca agaatgaaaa
     3961 ttgcattcaa acatattttg ccgaatatct ctagtgtcat tattattcaa atgatgttta
     4021 gtattccatc tgctattttc ttcgaagcct ttttaagctt tatcggttta ggtcttacgc
     4081 ctccgtcagc ttcattagga acgatgttga gcgaagggta caagacattc ctgtatctgc
     4141 cttatctatt atggattccg gcagtaactt tatcggttat catgattggc tttaacttac
     4201 ttgctgatgg attgcgtgat gcctttgatc ctaaaatgaa agagtgaaga cgatgacgaa
     4261 aattttagaa gtgaaagact tagaaatctc ttttgatacg tatgctggaa aggtacgagc
     4321 gattcgcggt gtcaattttc atttgaataa aggagaaaca ttggcgatcg ttggggaatc
     4381 tggaagtgga aaatcagtga cgaccagaag catcatgcgc ttgctgtcaa gcaacgccaa
     4441 tatagatgct ggtcaaatcc tctttaaagg taacgatatc gttggaaaat cagaaagaga
     4501 gatgcaaaaa atccgtggta aagaaatagc gatgattttc caagatccaa tgacctcttt
     4561 agatccgacg atgcccattg ggaagcaagt cgctgagtcc cttagaaagc ataataaagt
     4621 ttcaaaaaaa gagggacaaa aagctgcatt agaattgctt aaattagttg gaatacccga
     4681 agctgagaaa cgtatcaaca gttatccgca tcagttctca ggaggtcagc gtcaacggat
     4741 cgtgatcgcg attgcactgg tatgttatcc ggaaatcctg attgctgatg aacctacaac
     4801 agcgcttgat gtgactattc aagcacagat tttggagctg ctgaaagaaa ttcagacaaa
     4861 aatcgataca tcaattatct ttatcactca tgaccttggt gtagtagcga atgtcgctga
     4921 tcgtgtcgct gtcatgtatg gaggaagaat cgttgaagtt ggaacatcgg aagaaatttt
     4981 ctacaacccg caacatccct atacttgggg actgcttggt tcaatgccga cattagatag
     5041 tgcgaacgat cgattgtatg ctattcctgg ttctccaccg gatttattga acccgccaaa
     5101 aggagacgca ttctatccaa gaaatgaatt cgcgatgaaa atcgatgcag aacaagaacc
     5161 accatttttt gaactatcaa aaacacacaa ggcagcaact tggctacttg ctccgcaggc
     5221 acccaaagtg acaccgccag aagaaatcca acgtcgctgg gctatcttta aagagaaaca
     5281 aaaacatact tacggtcaag gaggaattgc tgatgccaaa taaactactt gaagttaaag
     5341 gattgaaaca gtatttcaat gtaggaaaaa agaatgaagt acatgctgta aatgacatca
     5401 gctttcacat ttatgaagga gaaacattcg gtttagttgg agaatctgga agcggaaaat
     5461 ccactactgg ccgtacgatc attcgcttga atgaacctac tgatggtgag atattattcg
     5521 acggtcaaga tataacgaag atcaaagata aaaaaggttt gacgaaattc cggcatgatg
     5581 ttcagatgat ctttcaagat ccatacgctt cgttgaatcc tcgtatgaaa gtcagagata
     5641 tcattgccga aggaattgat gttaatggac tagctaaatc atctgaagag cgtgcaaaaa
     5701 aagtcgatga tctattgaga actgttggat tgaatccaag ccacggtact cgttatcctc
     5761 atgaattttc aggtgggcaa cgccagcgta ttggtattgc acgagcattg gcagtaaaac
     5821 cgcgttttat catttgtgat gaaccaattt ctgcactaga tgtgtcgatc caagcccaag
     5881 tggtcaattt gttgcaagat ctccaaaaag aacatcaatt gacctatcta tttattgcac
     5941 atgatttgtc gatggttaaa catattagtg accgaatcgg tgtaatgcat aacggcttgt
     6001 tattagagat gggaacaagt gatgaaatct ataatcacgg tgttcatcct tatacagaaa
     6061 gtttgttatc tgctattcct ctaccagatc cagaccatga gagaaaacgc agaagaatca
     6121 aatatcaacc tgaaccagat gacggacaag tacgtcaact tcgtgaaatc gcaccagaac
     6181 attttgtcta tgcaactgag caggaagtcc catattacgc aaaaaaacta aaacgacaaa
     6241 aggaatctct tctggttgct aactaggagg gacatagatg ctaaaagctt ttaaattccg
     6301 tatgtatccg acagaagaac aaaaacagca attgattcgt acttttggat gtgctcgatt
     6361 tacctataac catttgctga aaaagcggca gaaaagttgg caacaaacag gagtagccaa
     6421 tttttcgctc acacccgcta cattaaaaaa agaataccca ttcttaaaag aagtagacag
     6481 tcttgcttta gcaaatgcac agcttaattt ggatcgggct tttcgcaatt atttcaaggg
     6541 acgtgccagc ttcccaaagc ttaaaacgaa aaaaagtatg tggcaatctt atacgaccaa
     6601 caaccaaacc cgtacgatct atctggaaaa tggctactta aagttaccaa aacaaaaaga
     6661 actaatcaag ataaatcagc atcggccagt tgaaggatcg attcgctccg ctacgatttc
     6721 tgctcgatat aatgaagaat tttatgtcgc actactttgt gatgtttcgc cagtaaaaaa
     6781 agaatcttta gctaaatgga tcgggatcgc ctatcatccg aagacattga tccaaacatc
     6841 tcgacctcta gaagtaactt taccaaaatt tcaccagaca gaagaaaagc tgcaacatgc
     6901 acagcggaaa ttgaatgtga aagtccgctc cgctcaccat agaaagatca ggttagatca
     6961 ggcaagcaat tatcagaaac aaaagagaaa agtaatggat ctttatctga aacaaaagaa
     7021 ccagcgagaa gattatcttg aacaattatc tgggaaattg gtcaaacaat atgactactt
     7081 atttgtagaa tcttttccaa aagaagaggc acatgctgat ttttctattc acgactggca
     7141 taaattaata acaaaattgc gttataagtc ccaatggtat aataaaaaat ttcttttgat
     7201 caataccgac ggagcagaag aaagcaattc cgtaagaaag agccaagtag tagaaaaaat
     7261 gggcagacat tcggttatca agggataaaa aatgaatgaa actgatagga gcgttagggt
     7321 tagccttgtt aataaaaagt cttaatgact tttgtttcta ggaatttttc ttttgaagaa
     7381 aagctcacta attatgacaa accaataaaa caatagcaga aaaagttcgt acgagctttt
     7441 tctgctattg ttaatttttt attcagaaag tttacgaata catcaaaatt ctgaaacaaa
     7501 aaacgtaact cacaaagagt tgtttttgcg ttttttcttg aatgattagg tctgaatctt
     7561 ttgctcacaa ccttattcat ggtgccccgg cttgagctga tagatttttg aaggacggcc
     7621 gattcctaca ggacgttcac cgatctcatc gaaatattgg agcatagctt tcttaaaatt
     7681 ggaatgatcg atcgttttat aatcgatccc taagaattta gcaaatactt ttcgagcttc
     7741 cgtaatcgtg aaatctttac ctaaaacttg tagaacttgc ggctcgtgtt ccattttatt
     7801 gactacacgg ttgaatgctt tcaagatgat ttgactatgg tcaaaagcca aagtatcttt
     7861 gccagaagac tctccagtga ctaaatcaag cgagatagcc acttctccgc tagataaatg
     7921 gatcttgtta ctatgacgtt ctagtgtgaa ccagcgtact tccttggcat catctccggc
     7981 gattagcggt tcttctccaa taaaggctaa gtagctgact gtgacgaccc agcctcgcgg
     8041 atctcgatta ggcgtactga atgtatgcag ttgctcaata ttttcttttg aaataaccac
     8101 accagtttct tcctttgttt ctcttaagac gctttctcca gttgactcat ccttttggac
     8161 aaagccacca ggcaatgccc atgaatttcg ataaggatgc ccttttcttt ggatcaacaa
     8221 aactttcagc tgatcttctt ctttgttata gcacatcagt acgatatcga cagtcaaaga
     8281 gggcttgtca tattctggac gttcctgttt ttgataccag ttaagaaatt cttcttcact
     8341 tgcttcatgt tcataatata atttttcttc ttctttagaa gcaaaacggt tcatctgacc
     8401 acctccattg acagtaaaag tactttttac cttattgtag cagactctga aaaaaaacca
     8461 aggatttact tgaaaaatct aggttatcat ttaaatatga aaaataaatg gaagtatgag
     8521 attgaataaa aagaaaagca gataaaacgg caatacttta taccgctctt cacgaatggc
     8581 aaagaagcca caaataacga tatatacgtc aatgataaaa cgcaaaatcc aagctgctgt
     8641 cataaaactg ttcaaatgcc catggaggtt aaaataacca tacatacgaa tataaaaata
     8701 gctgaatatt aaatgtgcta atgaaagaaa agcaatacta agtgtaaaaa aaagatgttt
     8761 cttctgcatg attgttccag tcccttacta agatagatag tctttattat accacgtgtg
     8821 aggggagaaa taaaaagccg ttttttagta gatatgaaac aaaacaaaaa cccgcattta
     8881 ttcaacctat tcatcctttt aaaggataga ttgaacgtag atagaagtcg tttatccagt
     8941 ctttttcatt ttgaaaaaaa gctaacagca tgcttttttt atggtatact agcaaggaaa
     9001 cgaattcgca tcatgcgata aaggaggaag agccccgtgg cagaaaagga aactttttat
     9061 atcactacgc caatctatta tccaagcggc aaacttcata tcggcaactc gtatactacg
     9121 attgcatgtg acgcaattgc tcgttataaa cggttgatgg gctttgatgt gttttatctc
     9181 actggagtgg atgaacatgg tcaaaaaatc gaaacaaaag cagaagaact aggtgtaaaa
     9241 ccgcaagaat atgtagataa aatggcggct gatgtcaaga aattatggaa aacgcttgat
     9301 atcagttatg ataaattcat tcgcacgaca gatgattacc ataaagcagc tgttcaaaaa
     9361 atatttgatc gtttattaga acagggagat atttacttag gtgaatatga aggctggtac
     9421 tctgtttctg acgaagaatt ctttacagaa acacaacttg cagaagtgta caaagatgac
     9481 gaaggtaacg tgatcggtgg taaagcgcca agtggacacg aagtagaatt ggtaaaagaa
     9541 gaatcatact tcttccgcat gagcaaatac gcagaccgtc tattggaata ctatgaagaa
     9601 catcccgaat tcatccagcc agaatctcgt aaaaacgaaa tgatcaacaa ctttatcaaa
     9661 ccaggattag aggatcttgc agtttcacga acaactttta cttggggagt accagtcaag
     9721 agtgatccga aacatgtggt gtatgtatgg atcgatgcat tatccaatta tattactgca
     9781 ctaggatatg gctccgaaga tgaaagtcta ttcgaaaaat actggcctgc agatgttcag
     9841 atggtcggta aagaaatcgt tcgctttcat acgatctatt ggccaatcat gctgatggcc
     9901 cttgatttgc ctctacctaa aaaagtattt gggcatggct ggctattgat gaaagacgga
     9961 aaaatgtcta aatcaaaagg aaatgtcgtt tatcctgaaa tgcttgtgga acgttatggt
    10021 ttagatgcac tccgttatta tttgcttcgt gctgttccgt ttgggtcaga tggtgtcttt
    10081 acgccagaag attttgtttc ccgtgtcaat tatgatctgg caaatgatct agggaactta
    10141 ttgaatcgaa cgattgctat gatcaacaaa tattgtgatg gactcgttcc tgactatgcc
    10201 tcacttgtca ctccatttga tagtgaactg tctacgacgg ctgctaacgt tgtcggacgt
    10261 taccatgatg caatggaaaa aatggaattc aatacagcat tggctgaaat ctgggtgctg
    10321 atttctcgtg caaacaaata cattgatgaa acagaaccat gggtattagc aaaagacgaa
    10381 gaaaagaaag ctgaattgaa tagtgtcatg atccatttag ctgaatcttt gcgtatcgca
    10441 gcgatattac ttcagccaat catgacagaa acgccagaga agatcttcaa tcaactagga
    10501 ttggactctg aaacaatgaa tcttgaagga ttacactttg gcgaatttcc ttcaggaaca
    10561 aaagtagttg caaaaggtac accaatcttc ccacgtttag atatggaaga agaagttgct
    10621 tttattcaag aaaaaatgtc tgaaggtact caaacaaacg aagatacagt caaatgggat
    10681 ccagaagaaa cagaacttgt ttcaacgaaa gaaaaacaaa tcaaatttga tgtatttgaa
    10741 aaagtagaac taaaagtagc agaagtcatc aactgtcaaa aagtagaagg ggcagataag
    10801 ttgctgcaat tccgactaga tgctggagac agccaagacc gtcaaatctt gtcaggaatc
    10861 gctgaatttt atcctgatcc aagcgaattg atcgggaaaa aagtcgtcat tgtagcaaat
    10921 ctgaaaccac gaaagatgcg tggacaaatc agtcaaggaa tgatcctttc tgcagaagca
    10981 ccagatggct ctttacaagt aatcgaagca ccaaaatcca tgccaaatgg ctcagaaatc
    11041 gcatagaaat tcaaaggggg tatgacacta gttgtgtcat accccctttt tccgaaattt
    11101 gagaagcaat tttcgtaaat tccttcttat tacttgaagc caatcatata atacagaaac
    11161 agagaactat ccaaaataaa tcctagaaaa agaagtacga gcttcgctga tttttgttaa
    11221 tttagaataa tactaatagg aatcgtaaaa acagaaaaaa agcataatga taaagggtcg
    11281 ggcagatttt aattgattca tctggtactc ttttttattg tccttacatt cataaagttt
    11341 gaatgcaatg actttattac atctatattt atatctaaca gtagcgttga tgtgttaatt
    11401 ataagttcta tcactttatg ttcaattagt ttgtgctctt atatgtgtga ttgaaaatat
    11461 agataaaaaa acaatcatcg gctttgactt cgatgattgt tttctttatt ttgtaaaaat
    11521 cctatcactt gattttatgg taacctttta tttttgtctt caaacggctt ggtaagagag
    11581 cagtaagcag tgcaaatggg atcgaaaaga cataccagaa aatcgtacgt ccgataccat
    11641 aatgttgata gagctttgtc aacatcacgt gtaacaagat caaccctaaa agcaaggagc
    11701 cagctataaa ccaatgatta tcccaatgca tgacttcact aagtgccatc actccaaaag
    11761 gatagaaata aaaatagatc ccgactaagg tcatccgcca gttctttgta aatgttccga
    11821 tattcaataa aaagaaaatc gctgaaagca aaataccgat aggcggaaaa agcccaacca
    11881 aaaaggaata caacatcccc cacaagatca tcacaggacc tttgatcaat gcaacgattg
    11941 cgatgaacaa agtcagcaat attagttttt gtgataacgt tgcaacagtc attaatccaa
    12001 ataaaattaa tgtgaaccaa tgccaatggg actgctgttg ttctttacgg gaaaaatcga
    12061 cccggttgga taaataatct gctaatttcg gataagaacg cttaaaatcc ataaactgtt
    12121 cctccttcgt ggaaaaggta ggtttagtat acgaaaaata aaaaagaaat acatccgtct
    12181 taagagggag tttttcactg aaagtggaga aaaaagaatg gtaaattaaa atattctcag
    12241 ttatactgaa aattatacca aatttcaaag aagaaagagg atgggaatga aaggcaaact
    12301 aaaacgagaa atcaatctgt ttggcgcatt agctaccgtt atgggaaccg tgatcggtgc
    12361 aggcgtcttc ttcaaaactg ctgctgttac tgctagtact cagtctacaa gtttgacatt
    12421 gctagcttgg cttttgggtg gtttcttaac gatctgtgct ggattgacag tggctgaatt
    12481 agctacagca atcccggaaa ctggtggagc tgtaaaatat attgaagctg cgtatggaaa
    12541 attgcccagc tttttattag gatgggcgca aagcttgatt tactttcctg ctaatattgc
    12601 tgcattatcg attatctttg cgacacaatt gacgaactta ttacaattat ctaccgatta
    12661 tcttttgttg attgcagtga tcaccgctgt ttctgtgaca gggttgaatt tgttagggac
    12721 aaaagtcggt gcttctgtac aatcagtgac gttgattgtt aaattaatac caattgctgt
    12781 gattgtcata tggggattat tgaccccagg acaaggaact gtccagttat ttccaatcga
    12841 agctggaaaa gatgttactt ttgtcgaagg attgagcagt gcgttgttag caacgttgtt
    12901 tgcttatgac ggctggttag gtgtcggtgc aatggcaggg gaaatgaaac ggccggaaaa
    12961 agatttgcct aaagcaatta ttcttggttt gagttttgta acagtcgttt atctattgat
    13021 caattttgtt tttttgaaaa ctttgccgat tgatcatctt gccggtaatt tgaatgcagc
    13081 ttctgaagca tcagacgtca tttttggagg aatcggtgga aagctggtca ctattgggat
    13141 cttgatctcg gtatacggtg cattgaacgg ttatacacta actgggatcc gcgtgcctta
    13201 tgcgatggct ttagaagacg agctaccttt cagtaaacag ctgacaaagc tttcaaagaa
    13261 gtttacggtt ccttatgttc ctgcggtttt ccagttagca gtagcttgta ttatgatgag
    13321 ccttggttct ttcgactttt taacagacat gttgattttt gtgatgtggc tattcagttt
    13381 actgattttt atcggtgtct ttattttaag aaaaaaagcg ccggaattgc cacgtccata
    13441 taaagtacca ctttatccaa tcgttccgat tattgcgatt ctcggtgcta ttttcatttt
    13501 aggtatgacg atgatgacgc aaacgaaatt agcattgatc ggtataggtg ttaccttgat
    13561 aggtatccca gtctattatc aaaaaaagaa aaaacgctca gaagagtaaa atatttcaac
    13621 aacagacaga aaaagtacga actaggtcct ctagatcttc gtgctttttc tatttcaata
    13681 aaacaagttc ttctattttg atttttgcta cttctgcaag tagtgcttat gtgctaaagt
    13741 aagaatgaaa aggagagata aacatgatat ttgattctca tacccattta aatgctgaac
    13801 agttcaatga agatattcca gaaacgattc agcgagcaca agaaatgggg gtaacgaaaa
    13861 tggcggtcgt gggcttcgat acgccaacga ttgaaaagtc tttgcaatta agccatgact
    13921 atccaaatat atacagtatt atcggctggc atccgacaga ggctggcagt tatacgaaag
    13981 aaatcgaaaa taaattacaa gaacaattga ctttaccaaa ggttgtagca ttaggggaaa
    14041 ttggcttgga ttatcattgg atggaagacc cgaaagaagt ccaagataaa gtatttcgca
    14101 gacagattgc tatcgcaaaa gaaatgaacc taccaatcag tattcatact agagaagcac
    14161 ttgaagacac gtatcaaatc ctaaaagaag aagatgttcg agatatcggt ggaatcatgc
    14221 atagtttcag tggtgattat gaatgggcaa aacgatttct tgacttaggg atgcatattt
    14281 catttagcgg tgtcgtcact ttcaaaaaag cattagacgt ccaagaggct gcagcaaatg
    14341 taccaatcga acgattactt gtggaaacag atgcccctta tctggcacca gttcctttta
    14401 gaggaaagcg aaatgaacca ggctatactc gttatacagt agaaaagatt gctgaacttc
    14461 ggaatcttcc gacagaagaa gtggctgccc aaacatggac aaatgcgcac cgattatttg
    14521 gtctaagcga acatggataa gatgaagatc caagagatta tagtcgttga aggaaaagac
    14581 gatacaagaa ggctgcggga agtagttgat gcagatacga tagaaacaat agggtctgca
    14641 atcaatgaag agatcctgat gcaaattgaa catgcccaag agactcgtgg agtgatcgtt
    14701 tttaccgatc ctgatttttc aggagaaaaa ataagaaaaa tcattatgga agctgttcct
    14761 caggcaaaac atgctttttt gccgcgcaca caagctaaac caaagaaaaa gggaagcctt
    14821 ggtgtcgagc atgcaagtga tgctgctatt ttagaagcat tgaaaaaggt cgtgacgcca
    14881 gacatttccc aaacggctgt ttctgaaatc actagacaag accttttaga ctatggttta
    14941 atagctgggg cacaagcaaa agaaaaacga gaaaaactag gtgacgagtt gcgcatcggt
    15001 tacacgaacg gcaaacaatt ggaaaaacgt ttgaagatgt ttcgtattac gccgaaagaa
    15061 ctcgcccaag caatgaaaat agtggaggaa caacatgagt gaatatcgtg aaattgcgac
    15121 accctctaga acaaaagaga ttttattgaa acatggtttt tcatttaaaa aaagtttagg
    15181 acaaaatttt ctgactgaac caaatatttt aaggaaaatc gtagaaactg cgcaaataga
    15241 tgaacatacg aacgtgatcg aagttggacc aggaatcggt gcattgacgg aacaattagc
    15301 caagcatgca aaacaagtcg tagcatttga gatcgatgat cgtttgatcc ctgtattggc
    15361 tgatacgatg caaccctatg acaatgtgac tattgtacat caagatattt taaagaccga
    15421 tttgtcaaca gctgttagag aaacatttca tgaggagctt ccgttaaaag tcgtagcgaa
    15481 tttaccttac tatattacga caccgatcat gatgcatttt cttgaatcag atttggttgt
    15541 ggatgaacta gtcgtcatga tgcaaaaaga agtagctgat cgtatctctg ctgaaccagg
    15601 aacgaaagct tatggttcat tatcgattgc tgttcaatat tatatggaag ccagtttagc
    15661 attcatcgta ccaaaaacag tatttgtccc tcagccaaat gtagattcag cgattttgaa
    15721 actgacaaga agagatatac cggcagtcga agtgacagac gaaaaagcat ttttccgatt
    15781 gacaaaagcg gcatttcagc aaagaagaaa aacgctttgg aataacttgc agcattcgta
    15841 tggcaaagat gaccagacaa aagcatggtt agcaaaaagc ttggaaacag ctggaatcga
    15901 tccaaaacgt cgtggggaga ctttgtcatt acaagaattc gccgcattaa gcaatgcgat
    15961 gtcagaaaat atgcagtaaa aaaagaggtt gtgacagaag ttgtgaaaaa gctgtcctgc
    16021 atcaagtaat aagaacagcc aaacaaaaat agttcctcat gtttttcgtt tgtttgggct
    16081 tattaccgca gatgcaagct tttgaacacc gtttaagaaa gaggttgtgg gcctgccgtt
    16141 taactctaag tcacaaggaa caatttcaaa aaacagcttc tcatgttttt gagttagttg
    16201 gtcttattac ttgatgcaag cttttgacca ctattatgcc gaacaaggag atgtgacaca
    16261 aattttgtca catctcctat ttttgtttct tgatcaaaaa attcttatac ttgatactca
    16321 cactcatttt gtatggattt agaaaagaat atgtgtcttg acagttatta gtaagtgagt
    16381 tatactaagt cgacatttat gtgtcaagac atattaggag agagaaaaaa tgagaaaaaa
    16441 ttcagttcga aaactaacga tttctgcttt attgattggg atgggggtca ttatcccaat
    16501 ggtcatgcct aaaatcatga ttccaccagc ttcctttact ttggcgtctc atgtgccatt
    16561 atttattgcg atgtttttta caccaggagt tgcagtggct gttgcgctag gtacgacatt
    16621 tggtttcttt ttaacaacgc cggttatcat cgcattgcga gcattgtcac atcttgtgtt
    16681 tgcgttgatt ggggcttggt acttgcaaaa gcatccgagt atcgttttaa aaaatggaca
    16741 gtttactttt gctaattggc gtttccaagg gttcaatttt gtgatcggtg tgattcattc
    16801 tttagcagaa atgctagtag tcagcatctt ttactttatt ggtaatatgc ctgaaacata
    16861 ttattctcaa ggatattttt atacagtatt catcttgatg gggattggtg ggcttatcca
    16921 cagtttagtt gattataata ttgcttattt tatagcagga acactcagca aatattttga
    16981 tatccccgta tttacggcag caaaaaaaga aaatcggaaa gagtcgattc aagggtacgc
    17041 gaagaaaacg acatattaga catgatctct caaaaaaaaa actctcatct agtaatggat
    17101 aataaatcct ctaagatgag agttttttgt cctaaaaaga gctgcaccaa catcttctgg
    17161 cacagctcta acagggggca atttatggaa gtttgatgac ttgttctgtt tctttatcaa
    17221 agaagtggcc tttgctgatg ttgaaggcaa ggtcgactat ttcgcctggt ttatggaaat
    17281 ctcttgcatc tacttttgag atgaactcag tagaaccagt ttttgtgtaa agcatcgttt
    17341 cagctccaag taattcggaa acgacaactt ctgatttcac tacggcttca ggtgaagctt
    17401 caagggctac ttgttcactg tggatgtctt ctggacgaat accgaagata acttcttttc
    17461 cttcataacc tttttcaact aatactttgt tttttccttc aggaatacga agcttaagcc
    17521 catgtccatc tgtgataaca ccatttttca atgtcacatt aaagaagttc atggcaggag
    17581 atccaataaa gccggcaacg aacatattga cgggtgtatc ataaacttct ttcggtgtac
    17641 cgatttgttg gataaatccg tctttcatga taacgattcg gtcagccata gtcattgctt
    17701 ctgtctgatc gtgagtaaca tagatggtcg ttgtttcaag acgctggtga agtttggcaa
    17761 tctcagcacg catggctaca cgtaatttag catctaaatt tgataatggc tcatccatta
    17821 agaagacttt tgcatcccga acgattgccc gtcccaatgc cacacgttgg cgttgtccac
    17881 cagataaagc agctggtttt cgtttcaagt attctgtcag acctaagatc tctgctgcat
    17941 tgtccacacg ttttttgatg tctgctttat catatttacg taatttcaag ccaaaagcca
    18001 tattatcaaa aacagtcata tgaggataca atgcgtagtt ttggaaaacc atggcaatat
    18061 cacggtcttt aggtgccacg tcattcatga cttttccgcc gatatttagt tctccttcag
    18121 agatatcttc cagtcccgcg atcattcgca aagtagttga ctttccacaa ccggaaggac
    18181 caacaaaaac gataaattca cggtctttga ttgatagatt aaaatcagtt acagaataat
    18241 tttctgcatt gtcatatttt ttatatacgt ttttcagagc catttctacc ataatacgtt
    18301 ccacactttc ttttttagtt ttattaacta tttaaagtat aaatgaaagc gttaactaac
    18361 ttcaatgtca tgttgaacaa gaattttttc ttttttttgt caatgtgcta gttttaagct
    18421 ttaataaata tccataagaa ataaaaactg atacactgga gtaaataagt atgttaacaa
    18481 tatcaaaagg agtgtctctt atgaaaatcg ctttgatcgc tcatgaccga aaaaaaccta
    18541 tgatggtgaa attagcaaca gcgtataaaa caatattgga aaaacatgaa ctttttgcta
    18601 ccgggacgac tgggcagagg atcacggaag caaccggctt gccgatccat cggtttaaat
    18661 ccggaccgtt aggcggggat cagcaaattg gagccatgat ttcggaggat cagttagatt
    18721 tggtgatttt tcttcgagac ccgctggctg cacagcccca tgaaccagat gtaacagcac
    18781 ttatccgttt atctgatgtc tatgagattc cgttagctac gaatatcgga acagctgagg
    18841 tgctactaag aggactggaa gccggcttcc tagattttcg aagaatcgtc catgaaatag
    18901 acaagcatcc actttcattt tgatggataa gcgtatggaa aaataacgtt taatgttcgc
    18961 ctatatgtag ctataaaagc taaacaaact gataatatgc gatataatac gaacgtattg
    19021 gataatataa acaataatat tagtttttcg tatttgcagt aaatgtgaaa atatgctata
    19081 gtttctgtgg gaaaagatcc caaaaataat acagagtaga aaataaagcg ttaagtgcta
    19141 agaggatggg atgttgcctc ttggacgaag gacgtaatgt tcgcggtttt attttcgcat
    19201 tcgctgcatg atacgatgta gcagctctta aaggagatgc taaaaatgaa gaaacaccgg
    19261 ttagacgcac gtatgattgc tattatgggc ttattgattg cattgatggt cactttgtcc
    19321 cgactggtag caatcgagac cccatttatt aaaatcagtg tcacatttat tccacaagtg
    19381 atcatgggca ttctttttgg tcctttttgg tcagggatag gcgcagtttt agctgattta
    19441 gtaggaatgg ccctcttctc gaaatctgca ttctttattg gatttacatt gaatgcattt
    19501 attgaaggag caatctacgg attctttttc tatcgaaaag agatcacttg gaaaaatgcc
    19561 attctggcta cgcttagtgt cacgctgatc atcaacttat ttttgacacc attatggctt
    19621 gcgttgatgt accatgtgcc attgttcagc tgggtcgttt gggcgccccg cctgttaaaa
    19681 acagtaatat ggttaccgat tcaatcaatt gccatttatt acgtaggacg tagtattcct
    19741 tacaaaaaaa ttttaagaag tttagcgatc cacgctaaat aaaaaacaag cagtcttccc
    19801 atcccagaag actgcttgtt tttattgtgc tgtgaattgt gcttgataga gccgtcggta
    19861 ataaccatct ttttgaagca gctcatcatg ggtgcccatt tcgacaatag ctccctgatc
    19921 catcactaat atctggtccg catttttgat tgtggacagg cgatgggcaa tcacaaaact
    19981 ggtttttcct tccatcattg ctaagaaggc ttgttggatt tttttctctg tcaatgtatc
    20041 gactgagctg gtagcttcat ctaagatcaa cataggggga ttactgatca tcgttcgagc
    20101 aatcgtcaac agttggcgtt gtccatctga tatctttaac ccttgaccgc caatcttagt
    20161 atctaatttt tccggcaaac gttctacaaa ttcatacata taagaggctt ttagggcatc
    20221 attgatttct gcgtcacttg cttccggatt cccaaaacga agattttctc gcaatgtact
    20281 gtcaaatagc caagtatctt gtaacaccat tccaaaactt ttacgtaatg tatcacgttg
    20341 gatagagcgg atgtcatggt catcgactgt aattgttcct tttgttgtgt cataaaaacg
    20401 catcagcaaa ttaacaagtg tggattttcc ggcccctgtt tttcctacta tagcaatggt
    20461 ttgacctgga taagcagtaa aattgaaatc ttcgatcaaa cgctgcttgg ggtcataaga
    20521 aaaagaaaca tgatcaaaag agacttttcc tttgatattt ttgggttcca aggcataggc
    20581 atctggcaaa tcaggctttt cttctggctg atcgatcaaa tcaaatgctc gttctagacc
    20641 agaaaaagcg gtttgcagtt gtgtcgtgat tccagaaagt tcgataaatg gtttagaaaa
    20701 ctgacttgag tagatcgtaa agctggaaat cacaccaaca gtgattgacg aaccactctt
    20761 caatgccaat aggccgccaa ctaaaccaat cgataaataa gcaaggtgat ctacaaaacg
    20821 agaagacggg tttgtcaagg aagaagaaaa ttgtgcccgt tgtccttttt catatagatc
    20881 agcatttagc agttcaaatt gctgttgact agcttcttct tgctgaaaag ctttcacgat
    20941 tttctggtta ccgattcgtt cggtcacaaa gcccgagata tccccaataa tcctttgctg
    21001 tctatcgaaa tttgtttgtg aagcacgagc aacgatccaa ctcacaagga aaataattgg
    21061 tgtagaacaa agaacgacca atgtcagaag tggacttaga cgcatcataa aaataaacga
    21121 aagaatgatg attgccactc cagaaaataa ttgattgaag gccgcagaaa ttgcaatgga
    21181 aatattgtcc atatcatttg taaatcgact gacgatattt ccgtgggagt tttgatcata
    21241 ataacgaagt ggcaatcgat tcagatgagc aaatgcatct tttcttaatt ttgcaacgga
    21301 taagtaagct aaccgattgc ttaacacttg gatgagccat tgactgacta cgttgattaa
    21361 aacgatacca ccgaatagaa taagtaaatg gaacagtcga ttgaagcgga cctgtccttc
    21421 tccaatcatt tgatcgatac tttgaccaat ttgtaacgtc atcacaactg ttgcaatccc
    21481 attgatgatt cctagtatca cagctagcca aatgtctttt gcataggcag taaggtaagg
    21541 aatgaatcgg cggagcgtct gcaaggataa tttttttact ttcataacta ttcttcctcc
    21601 tgtgatgcga cgatttcctg atattctgct gattgagcaa gtaattcttc atgggtaccc
    21661 tgagcagcaa tcttaccttg atccatcact aagatatgat gtgcttcttg aatagagcgg
    21721 acacgttggg agataataat cactgtactg ccaagataag tctgtagcgc cttacgcaaa
    21781 tccaagtctg tttgataatc caacgcactt aaagagtcat ctaaaatcag taatgctggc
    21841 ttggcaatca aagctcttgc aatcgtcagt cgctgttttt gtccgccaga gaaatttttc
    21901 ccattttcca tgacttttgt atctaaacca tttggaagct gacggacaaa ttcttctgcc
    21961 tgagcgattt tcagagcttc ccaacattct ttttctgtgg cgtctttctt accccattga
    22021 aggttttcac gaatagtccc ggtaaatagt acagaagttt ggggaacaag agcaatttgc
    22081 tgacggagat gatccagctg ccaagaacga acatccattc cgtccattat aactgtaccg
    22141 gaactgacat catagaaacg cggaatcaat tgtgtcaaag tcgttttccc gcttccggtt
    22201 ggaccaatga ttccaaggac tgtatttcgc ttcagctgaa atgtaatatt ttggagagaa
    22261 agaccagaat caggagtata acggaagtca acatggtcaa aagcgataat tgcttcagaa
    22321 gaaaaatcgg gagtagtact gacctcagct tcaagaatcg aattttctgt gtctagcacc
    22381 tctttcaccc gatttcctga agcttcggca cgtgtgaaga tcacgacgag gtttgaaaca
    22441 acgatcagtg ccaaaagcat ttgattcata taattgatca aagcaagaac ttgtccttgt
    22501 tgcaagctac caatatttac tttgatcccg ccaactgtca gcaaacagat gatcccgaca
    22561 ttcatgatca aggttgtagc aggtgataat agggcagaga tattggaaac acgtaaataa
    22621 ttgttcgcta gatcatttgt gcttttatcg aacttctctg tttctttttt tgtctgcgca
    22681 aatgcgcgga tgacacgaac accgctaaga ttctgactaa cgtgttcatt gagctgatct
    22741 agtttttctt gcactttttg gtagagagga acggtttttt tgatgataaa aaataaaatg
    22801 attgagaaaa taggcaataa tgctaagaaa aacagaccta cttcccagtc gatcacaaaa
    22861 gccatgacca ctgacccgat acttaaaaac ggtgctctga tcaataaacg aatgaccatt
    22921 gctaaagcta gctgaagctg attgatgtcg tttgtcattc gggtgatcaa ggtatctgtt
    22981 ccaaaatgat tcaactcgtt gtgagagaaa gtattgattt ttttgattag ctgattacgt
    23041 aactctgtac caaatccttg tgaagctact gaagcatagt attggcaaat gatagcacaa
    23101 atcagaccaa tcacagacat agcgaccatc cataagccca tttcaatgat ttttcctcgg
    23161 tcattcattt ttagtccatt atcgatcaag gaagccatca ttaaaggcag gacaagttcg
    23221 aagacagctt caagaaattt gaagaacggg cccaaaatga tttgcttacg atagtctttt
    23281 gcataacgta ataactgaat catatgattc ctcctattat ttatttgtcc acaattgaaa
    23341 aaacaagtac taccacattg tagcttagga aatttagaat gaaaagagaa caagcctttt
    23401 cataaatgaa agttttatta tgaagaagct ttagaaattg aatagactat tttttttaaa
    23461 aaaaagaaaa tgagaaaaca tgc
//
LOCUS       DACECZ010000032        23359 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.032, whole genome shotgun sequence.
ACCESSION   DACECZ010000032 DACECZ010000000
VERSION     DACECZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.032"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..2144)
                     /locus_tag="IWO35_001189"
     CDS             complement(<1..2144)
                     /locus_tag="IWO35_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0316258.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2501..3874)
                     /locus_tag="IWO35_001190"
     CDS             complement(2501..3874)
                     /locus_tag="IWO35_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0316259.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3874..4269)
                     /gene="rbsD"
                     /locus_tag="IWO35_001191"
     CDS             complement(3874..4269)
                     /gene="rbsD"
                     /locus_tag="IWO35_001191"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ0316260.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4297..5214)
                     /gene="rbsK"
                     /locus_tag="IWO35_001192"
     CDS             complement(4297..5214)
                     /gene="rbsK"
                     /locus_tag="IWO35_001192"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ0316261.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5322..6335)
                     /locus_tag="IWO35_001193"
     CDS             complement(5322..6335)
                     /locus_tag="IWO35_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0316262.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6416..7015)
                     /locus_tag="IWO35_001194"
     CDS             complement(6416..7015)
                     /locus_tag="IWO35_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0316263.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7201..7902
                     /locus_tag="IWO35_001195"
     CDS             7201..7902
                     /locus_tag="IWO35_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0316264.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7912..10623
                     /locus_tag="IWO35_001196"
     CDS             7912..10623
                     /locus_tag="IWO35_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0316265.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11074..12702
                     /gene="treC"
                     /locus_tag="IWO35_001197"
     CDS             11074..12702
                     /gene="treC"
                     /locus_tag="IWO35_001197"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ0316266.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12762..13943)
                     /locus_tag="IWO35_001198"
     CDS             complement(12762..13943)
                     /locus_tag="IWO35_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0316267.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14195..15718
                     /locus_tag="IWO35_001199"
     CDS             14195..15718
                     /locus_tag="IWO35_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0316268.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15787..16514)
                     /locus_tag="IWO35_001200"
                     /pseudo
     CDS             complement(15787..16514)
                     /locus_tag="IWO35_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(16527..17807)
                     /locus_tag="IWO35_001201"
     CDS             complement(16527..17807)
                     /locus_tag="IWO35_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0316269.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17948..18550)
                     /locus_tag="IWO35_001202"
     CDS             complement(17948..18550)
                     /locus_tag="IWO35_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ0316270.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18795..19001
                     /locus_tag="IWO35_001203"
     CDS             18795..19001
                     /locus_tag="IWO35_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316271.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19313..20032
                     /locus_tag="IWO35_001204"
     CDS             19313..20032
                     /locus_tag="IWO35_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0316272.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20113..20796)
                     /locus_tag="IWO35_001205"
     CDS             complement(20113..20796)
                     /locus_tag="IWO35_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ0316273.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            21014..23200
                     /locus_tag="IWO35_001206"
     CDS             21014..23200
                     /locus_tag="IWO35_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0316274.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         7771 a         4206 c         4585 g         6797 t
ORIGIN      
        1 tatttcttta aaggcagatc aagccagaat gatttgtcta aacttaacaa ggttagtaca
       61 ggaacagttg cagctttaac tgtacgagta gttgtttctg gtatctgtct tgccgtaaaa
      121 ttgttccaca aatcccagat tgaagcgaca cggaagatgg aagaagcgtt gttgacgcgt
      181 tttatcgttg aaaattctaa tcctgtgatt ggcacgccat ctgttgtcca tttgattggc
      241 acatcgaatc gcttagacat attgaggatt tcagcgtttg cttcttcagt tgttgctgct
      301 tcttttttcg gttcatcata ttttgtcagg tcgtacgctt cgatgatcgc gttcaatttt
      361 tgtgaatatt ggcgatctgt agcataacgt ccttccaaat atttggttgc ttctttgtag
      421 tcttttgttt ccgacttcca agttggtttg tagaaatcag gattgccagt cagtccattt
      481 ttgatcaagt cggcatagtc ttccaaactt tccttgtaag aaggatattt acggaattgt
      541 gcgcggattt ggtagctttg tccggaacta tcttgttcaa gtgtattgaa ggtcacgctg
      601 cttcctttat atgatccttt gataccaaaa aggttatgat ttggttcgct tgataataag
      661 ctgtttccag atccactttc aaggatagct tgagccatca tgactgatgc gtagattcct
      721 tctttttgcc cgatttcctg tgcatcatct gcaatctttt tcaagaactg atcagttgtt
      781 tggttttgac tgaaatggaa cgagccatct aatccatcta aaatagatga gccgcctgtc
      841 agcgaacctg aagcagcagg taaacctgct aagtttactc ggacagcaaa atcaggcgtg
      901 aaatctgaaa tagaagttac tcgaacttta tctcctggtt gaggcgcatg gataaattgt
      961 ccgttcccaa tatagatacc tacatggtga gtaacaccgg aagaaggtcc ccagaagacg
     1021 aggtcgccag gttgggcctg acttactgga atctgcgtac ctgcatattg ctgttcgcct
     1081 gtccagccac cgatataatg accggttgct ttcatatata cgtaataagt caaaccagaa
     1141 cagtcaaaag tatttggacc ttttgctccc catacatatg gttttccaag atgcttgaat
     1201 gcttctcgaa cgattgcttg ttggacattc gttgtatttg taggcaatac agcatcaatt
     1261 ggtgatttta caacaaaatc agaagaagca gaagatggat tctctatacc gctttgattt
     1321 gctgtatttg gacgagatga agcattgttc gttccgccat tcgatgaacc attgtttgga
     1381 tggtttggtt ctctctgagt atttccattg ttattcgatc cagatgggtt tgtttgattc
     1441 gaagaattcc cgctgttcga attattgtta ttattgtttg aattatttga accattatta
     1501 ttgttgttat tacccacatc tggtttgttt ggcgtttccg gatctggtgt actagggact
     1561 tcttcactag gatcagttgg tttctctgtt gaagtatccg gtccagcttt aaattctgca
     1621 gtaatagata aattgttcgt acctactgta aaggtctttg catcataatt tacttctttg
     1681 gcaccattaa cagcgactga agcaatttgt tcaccttcag ctggcgtaat agtgtaagtt
     1741 accttcgttt ccgctaataa accttctacc gttccacttt ccgtaatatc caaaggttgc
     1801 tgttcagggg aatccgctgt cgaagcagtc agttggatct taccaatcaa agaaggagca
     1861 attgtaaggg aatatgtctc ggctggcttt tcattttctg atggatcagc cggatcaatt
     1921 tcagttggct cggtcgatcc tgttgttgag ttagttgttc cagttgggtc ttctgtaaaa
     1981 gaatccgttg tttcactagt agctgagctg gatgtagtcg tatctgttgt gactgtttga
     2041 tcagctattt gattatctac ggcaaatacg gtattggtcg acaaaacaga cggagcaatc
     2101 agccaagtag cagctagcaa acgtatcgtt tttttatttt tcataataaa ctagtccttt
     2161 tcctttcatt gattttctgt cttattcagc attcattaga cattttttgg aacagataat
     2221 cgtgtcgaaa agctttttct tttcataaat ctgtctgtgt attctaaata aaacaatttg
     2281 taaataagca acgagcattc attcgttttt ctttgacgtt cattttattg catagaccac
     2341 ctttgtttgg ggataaacaa gtggggataa aaatggttat gcatatgtaa attataccaa
     2401 agtttggaaa gtatcacaaa aatttataaa aataatgatc ctaaaaagaa aaagactgaa
     2461 atatgtgata aacatctcat acttcagtct ttttcattaa ctattcataa gacattaggt
     2521 cttcatcaaa cccgctaacg agagtagtga tcattgctac gactagacta acagccagtc
     2581 ctgctaagta ccagaaaaaa gtaccggaaa tgaatgcagg caatgtggtc agactaccaa
     2641 atacaaaagc gatcgctttc acttgggcaa atcccattac agctccacca gcagctgcac
     2701 caatgcaagc agcaacaaat ggttttttca atgggatcgc aattccgtaa acagttggtt
     2761 cggtcactcc cgcaagtaaa atcgaagcaa aagcagaacc ggagatcgaa cgggtttttg
     2821 aacgtttact tcgcagaaat acacctaaag ctgcaccagc cattccaaaa ttggctgctc
     2881 ctgtcaacgg aacgattcgg tcgaacccat aagcagagat attactgatc atgacagggt
     2941 ttacagccca ttggatacca aatattacca aaatattcca accaccagcg atgattgcgc
     3001 cactgatcgg tccgttaata tccatcatcc aagtaattgc ggcagctaaa ccttcgccaa
     3061 ggtaaacacc cgcaggcccg aataccatta acgtcaaagg aaccgtgata actaatgaaa
     3121 gtaaaggtac aaatactaat tgcatatttt cgcgaatata ttttttgagg aatttttcaa
     3181 caaaagaata aaagaaaata cctaaaatcg ccggcatgac agtagacgta tactgcatca
     3241 gtactacagg aatccccaga aaatcagtag ctgctttgcc actgtctgaa agtaaagagg
     3301 tcagattcgg ttcaatcagt gctgcagcaa tgacagccgc aatatatcca ttcactttaa
     3361 acgttttggc agcagaaaaa gcaagaaaga ttggcagaaa ataaaagacg gcattcgacg
     3421 cagcagacaa aatatgataa gttccgcttg tttcactaag ccagccaatc gtagtcgcta
     3481 aaacaaccag accttttaat ataccagaac cggcaaacag cggaatgatc ggcgtaaaga
     3541 tttccgatat caaaccaaat agtttttcga tcggatttag cttttctttc ggagctgaat
     3601 gatcgatttg acgcgtcaat tccttgtaag caggagccaa tccctgatcc atgattattt
     3661 ctgcatatct atcgagtaaa atgaaatact taatggaagg gatttgctgt tttaattttt
     3721 ctgcattggc tactggctgt tttttcaatt tgatacgaag ccgagtcgca catggaagga
     3781 cagaatcaat attgtctttt cctcccaact gttcaatcag catatgaatc tgctcggaca
     3841 gtaccatctg ttcaggattc attgtcgttt ccattagaag accactccac tcactaatat
     3901 aatattggcg taaggtgtca tttcgcctgt acgaataaat gcatgacagt tattcaactc
     3961 ttttttcatt tcttcatgtg ggataaagct aatcggtgta tctggtaaca ataatttgat
     4021 tttttccaat atttccgggt tcattgtctt tatttcttcc gccaattcaa tcgattgtac
     4081 ctcaagttct tctaaaacgt tttctaaaac agataaaaaa cttggaagac cgtttgagac
     4141 agccaaatct attttttcag taccaaaggg gacaggcatg cctgcatccc cgatacttaa
     4201 tttatcaaag tgccccattt gtgcgattac tcgtgaaata tctgaattga ttacttttcc
     4261 ttttttcatg tttgtttctt cctctcattg ttaattttag actgctgatt gttctaaagc
     4321 ttcgcggtaa gggatagaag gttgcgcacc ataagactgc acagcaacag aagaggctag
     4381 atttccataa aggatcgctt ctttcaagtt actaaggtct ggctgaagtg tgctgcttaa
     4441 tgcgccaata aatgtatcac ctgcggctgt tgtgtcaacg gcatccactt taaaagcagg
     4501 gacgataccg tgttcttttt ctgtatgata gaaagcgccg gcactgccta gtgtaatgat
     4561 tactgttcca atacctaatt catggagttt ttcggctgct gcgaccaaac tgttatgatc
     4621 tgtcactctg attcctgtaa tgatttctgt ttctgtttca tttggtataa tgatatctgt
     4681 cttttcaagt agttcagtag gaatcgtttc acgagctggt gcaggattca ggatcgttgt
     4741 ttttcctgcg tcttttgcaa tagaaaaagc taggatcgtc gcatctaagc tactttcaaa
     4801 ctgagcaata ataaagtcac tgtttgtaat cagttgcttg ttttttagga cttcttgttc
     4861 atggaaagca ttatttgccc ctgcatggat caagatacta ttttctcctg ctgcgtctac
     4921 cataatcatt gcttgtccgg ttgtggcgcc ttggatttca gctatcccag atgtatcgat
     4981 gttttctttt gtcagtagat ctaataactg ttgtccttcg ctgtcagccc caacgcctcc
     5041 aataaaagat gtccgtgcgc ctgaacgttt ggctgccact gcttggttgg cacctttacc
     5101 tccgccagcg tgaaaaatct catgcgcatg catcgtttcg cctggcttgg gcatgttggt
     5161 taaacgaagg gtagtgtcca tattaatact tccaattact gtaaccgtgt tcattttaca
     5221 aacctccaac tatctcattt attttgatgg gaaaattatt ttttaaaacg ttttagtaaa
     5281 acgttttaat agttcgataa aaatatagca caccttgaaa gctatttcaa gggtgcagtt
     5341 gaaaatcttt tttctaatcg tacaggtaat cttttttctt cccattcttt ttctgggaat
     5401 tggatccgat ctaacaataa cttggcagat gtttgtccca attcgaagat tggttgtgcg
     5461 atcgttgtca gtttcggctt gatatactcg cacatatcta tattatcata gccaataatg
     5521 ctgtagtctt cagggatgct ttttccagct tcctctaatc cgcgatataa tccaaaagct
     5581 aattcatcat ttaaagcaaa cacaccagtt gcagcagaat caagaagttc tgctgtgatc
     5641 tggtagccac cttgttttga gaactgtgta ggtaagagga tcagttggtc gtgagggatt
     5701 tggtaaacat caagagcaga tttaaacccc tcaatccgag cgtggacatt ttccggtgga
     5761 ttttcaggat agacaagggc gatcgtttga tgtcctagac taagcaagtg catgcccgct
     5821 aggtaaccac cgcgaaaatc gtctgttcgt acggcatcac taaatccttc agattttttc
     5881 tgatccaaaa caatgaaggg ccggccttgt tttttcaaat tttcgttgat tgcatctgta
     5941 gagacagcag aagtcgcgat aatgaatcca tccacacctc gacgtgtgag ctcggctaga
     6001 tattcgattt ctttttgatg atcagaatca gcattacata atatcgtgac gaaattctgc
     6061 ttatacaaga tatcctcaat tcctcgcatc agtgtactga agaatggatt cgtgatatca
     6121 ggaacgagta caccaattgt tttcgtttcc ttggtaatca tttgtctagc aaaatagtca
     6181 ggctgatagc ctaattcttc tttggcagct aaaactttct gaacagtttt cggactgaat
     6241 ttctccgtat ttccatttaa tatcaaggag acagttgtga ttgaaacgcc ggaaagttta
     6301 gcgacttctt tgattgtgat cttttttttc accatgttca tactcctatc taaatcctgt
     6361 ggttattttc ccatgaaatc aaaaagaagt aaatgaaaat cacgtgtgcc agtacttatt
     6421 tgctttgttg gatagcttcg attatttgtt tcgttgagcg tactcgtgca gttttcggaa
     6481 atatcttttt tatgaacaat tgatgggatt ctttgtcacg atcactgcaa gcatcttcag
     6541 caagcacgat atggtacccg tgttgaaagg catctaatgc agtagcatat acaccgtttg
     6601 ttgtcgctac accgcttaaa atgatagttt cgattccacg ccggcgaagt tgaagatcaa
     6661 gagaagtacc aaaaaaagca cttggattgt attttgtgat agtcaacata ttatctgtat
     6721 ctttcaaaga atccttcaaa agtaaatcag tgaattctgg aggtacagaa gcagtcattc
     6781 cacccatatc agtttgttga ctcaaataag ggaaagtgga gatgtctacg ttcactaaaa
     6841 ctttcaaggc agcggtacgt ttcaatgttc ttactaacaa atcattgttt gtcagaatag
     6901 atgctgcagt gtgtggagag agtgtaccgt tatttacgat ctctttttgc atgtcgatcg
     6961 cgacaaaagc agtttttgat aaatcaaaaa tcatattttc ctttaaatca ttcatgatgt
     7021 tccttctttc aaataacaat gttttttatg taagtaaatc ataaatcgtt tatttgtcaa
     7081 ctaaattaaa ggattccttt ctttagtcta gagaaattca gtggtaaaat aaataagtgt
     7141 gagaaaacgc attcgatgca tagatatagc gagaaatatc gtgaaaatgg aggaatcaga
     7201 gtgaagtatg tcgaagtaaa aaatgaatac aaacgataca aaatgggaga aacgaccatt
     7261 acagcaaatg acgatatttc tttttcaatt gaaaaaggcg aattagtgat tatacttggt
     7321 ccaagtggag caggaaagtc aacgattctt aatattttag gtgggatgga tacacctgat
     7381 gaaggtcaga tcattattga tgacacagat attgcgcaat tttcagataa acagcttact
     7441 gcttaccgac gaacagatgt agggttcgtt ttccaattct acaatctcgt tcctaatcta
     7501 acagcaaaag aaaatgtgga attggcaacg gaagtttctc ctgatgcgct tgatcctgta
     7561 gaggtattga ggcaagtggg cctagctcat cgattgaata actttccgtc ccaattatca
     7621 ggaggcgaac agcagagggt ttcaattgct cgtgcactag ctaaaaatcc caaacttctt
     7681 ttatgtgatg aaccgacagg cgcgctggat tttgaaacgg gcaagcaagt attgaaatta
     7741 ttgcaaaacg ctagcagaca acacggcaat accgttttga tcattacaca taattctgct
     7801 ttagcgccca tcgctgatag agtcattcac attaatgatg ctaaagtaag gtctgttgaa
     7861 ttaaatgatc atccttcatc cattgatgag attgtatggt agggggattt tatgaaaaca
     7921 agaacatatc ttaaagcagg ttttcgcgaa attcggcaat ctaaaggacg attcattgca
     7981 atcatcctta ttgtcatgct aggaacactt ttatttgtag gtgtcaaaac tgctggacct
     8041 gtcatgcaaa aaacgatgga tcactatgtt ggtacagcag gactttctga tttgcaaatc
     8101 gtttctactg gtggcttgac acaaaaagat attgctcaag cagaaaagat cccagatgca
     8161 aaagtagaaa caggaaaaca gatctattat tccaatactg gcaaaaacga agtgattcaa
     8221 atattttctt ataacaaaaa taacaagcag aacaagctac aattaactga tggaaaactc
     8281 ccggaaaaac caaaccagct tgtattggat gaaaaagcgg aagatgaagg gtataaaatc
     8341 ggtgatattt atcagattga ttcagatgaa ttgaaagaaa aagagtatac catcacgggc
     8401 tttgtccgtt ctccattgtt catcaacaat ttggagagag gatacgcgaa cgtcggaaat
     8461 ggtagcgtcg attattttgt ctatttacct gaatccgttt ttaaaacaga tatttattct
     8521 gtcttatatc ttgattttac aaacgtaaag aatttgaata cgtacagcaa agcttacaaa
     8581 gataaaatgg agcaaaatca agaaaaggca gaaaagtatt tacaagatcg acccgaagaa
     8641 cgcttagaag agctgaaaaa atcggccaac gaagaattag aaccggcaca gcaaaaaata
     8701 gcggatggca aagcacaaac aacgcaagca agaacacggt tagaagatgc taaaaaacag
     8761 ttagcgcaac agtcagcaac tatccagcag cttcctgaag cgcaacgaac aatcgctgtt
     8821 caaacattat ctcaacaaga agctcaactt gctgaacaag aaaggcaatt agttgagaaa
     8881 gaacgtgaac tggcctctgc agaggaagaa ttgaatcgaa atgcagaaaa aattgaaaat
     8941 ttaactaaac catcatacct atacaatgaa cgttcagaaa atgtcggttt tcaagaattt
     9001 ggtgatctgt ctgatcgtat cgctgcgatt gccaatgttt ttcctgtttt ctttttcttt
     9061 attgccgcat tgatcacctt tacgacaatg acacgtatgg tagaagaaaa ccgcagggaa
     9121 atcgggacat taaaggcgtt aggttatacg aaagttgaaa tcgcaaaaaa atatgcaatc
     9181 tatgcaagtc tagcctcagg gataggtatt gttttaggaa cgatattagg aacgaatctt
     9241 ttgccaagga tcatttttga gctgtctaac gagcggtatg atattgggag tgcgcttatt
     9301 ttttatgact ggccgccgat tttacaagca gctgccgcat tcttcattgc cgcatttggc
     9361 gctgcaatga tcgttttatt caaagattta agagaacggc ctgctgcatt gcttcaaccg
     9421 aaagcaccga agccagggaa acgcattttt cttgaataca tcactccgct gtggtcacgc
     9481 cttagcttta atcaaaaaat cagttaccga aatctcttcc gatataagtc aagaatgctt
     9541 atggcgatta tcggaattgc tggatgtgcc gggctgatgg ttgcaggcgt gggattgaaa
     9601 gattctctaa gttctgtttc agccaaacaa tttggaccga tcattgacta tcaagcaatc
     9661 gttacattgg attcatccac ggaagatagt cgtcaaaaag cagcggatat gctgcagaag
     9721 caagaaaaag taaatgagat cctagctatt catactcaaa ccgtagaatt gagaaaaaaa
     9781 ggacatgctt cgcaaagtct gacagtgatc gtaccaagta aaacagctga tttggatcgt
     9841 tttatccatt taactgatct tgaagagaac ccacgctcct tgcctaaaac aggaatcgct
     9901 attacgcaaa aagtagcaga actatttgac ttatctacag gagatcatat ttcgctttat
     9961 gatgaagatc aaaaagaatg gaaagtcgaa gtaaaagaaa tcgtgaaaaa ctatctaggt
    10021 agctttattt atatgtcccc tgaatattat gcttcagtta ctgatcaatc gatgatggat
    10081 aatgcatttt tgctgcgtac agataagatg acaacaaaag aagaagaaat gctttccgag
    10141 aaactgcttg ccacagacgc tgtgacaaat acgtctttca tgtccaaaca aatcgaaacg
    10201 caagaagaat caatggcgaa cttagactcg gttgtcttaa tctttgtcgt attatcagga
    10261 ctgctggctt ttatcgtctt gtataatcta actaatatca atatttccga gcgtgtacgc
    10321 gaattatcta caataaaagt ccttggattt tttgataaag aagtcacgat gtacattgtt
    10381 cgagaaaata tcatctttac tcttttagga atcatcggag gatttggtgt aggatatata
    10441 ttgactgact tcatcttgca acaagcgtct atggagaatg tcatctttcc attagtgatc
    10501 acatggtggg cttatgtctt atcagcagga ttaactatcg tatttacggt tattgtgatg
    10561 attgttaccc atttcaaatt aaagcatatc gatatgattg atgcgttgaa atcgaatgaa
    10621 taaaaacaga ggttgtgaaa tcagcggttt gacctgagta ttagagaaac aaacggcaaa
    10681 atagtttttc atattttgcc gttttttgag ctaataccga aggacagctg attgaacacc
    10741 atgaatcaga ggttgtaaaa aagccgtcct gcatcgagca aaaagaatac tcaaataaaa
    10801 ttagctcctc atgttttaca tttgagaaga ctgattgccg tagataaaag cttttgaaca
    10861 ccatttatag aagaaacaag aatattttgc tattttaaac aagaggatag acaaatacat
    10921 gtctgatctc ttgtttttta ttgggcaaca gacctcttag aatgtataaa gataaatttt
    10981 acaaacgctt tcggttcagt tataataata aatgataact tgtatatata agttgtggta
    11041 ggtaataaat cagttgaatg gaggtaaata tccatgacat ttaaagacaa agtcatttac
    11101 caaatctatc ccaaatctta caaagacagt aatggtgatg gaattggaga tttaaaagga
    11161 atcaaagaaa aactcccgta tctgaatgat ctaggcatcg acatgatttg gctaaaccct
    11221 atttatccaa gccctcaaaa agacaacggt tatgatatct ctgattacac agccatcgat
    11281 cctatctttg gtacgatgga ggaattcgaa cagctaatag ccgaagcaaa gaaataccgg
    11341 atagaaatca tgctcgatat ggtgctgaat cacgtttcga cagaacatga atggttcaaa
    11401 aaagcactag caggggacca gcattatcag gacttcttca ttttaagaga agagccaacg
    11461 gattgggtat caaaatttgg tggaagtgct tggtcgccat ttggagatac ggggaaatat
    11521 tatttgcact tatatgatcg gacacaagct gacttgaact ggcggaacca agcagtaaga
    11581 gaggaactat tcaaagttgt tcgtttttgg atggaaaaag gggtaaaagg attccgtttc
    11641 gatgttatca atgttatcgg taaagacgaa caattgaaaa ataataaaga aaatgatggc
    11701 aaacctgaat atacggacaa accaatcacc catacgtatc taaaagaatt gaaccaagca
    11761 acgtttggga cggattccac tatcatcaca gtcggtgaaa tgagctctac gacgatcgaa
    11821 aattgtatct tgtatacaga acctagtaga gaagaattat caatggtctt taatttccat
    11881 catttgaaag tcgattatga aaatgggaat aaatggtcta ctcctccatt tgattttgca
    11941 gaattaaagc gactatttca tacatgggga gaaaagatga gtgagggtaa cggctggaat
    12001 gctctatttt ttaataatca tgatcagcca cgtgcgctca atcgatttgt agctatcgat
    12061 cagtatcgaa aacaaggagc agagatgcta gcaaccatga tccatttgaa ccgtgggacg
    12121 ccctatattt atatgggaga agaaatcggc atgatcgacc cagactattc atcgatttcc
    12181 gactatattg acgtagaaag tttgaatgct tataagttac tccttgaaca aggattcaca
    12241 gaaaaagaag cgtttcgtcg gatcaaagca aaatctcgcg ataactctcg aacacccatg
    12301 caatggtcag acacagaaca agcaggattt actacaggga aaccttggtt aaaagttgga
    12361 ggcaagctag aagagattaa tgtagaaaaa gaactatctt ctgaagattc tattttgtcc
    12421 tattacaaga aattgattcg gctgagaaaa acatatccaa tcgttgcaca aggagattat
    12481 catgcttatg aagcaaatca tccggaagtt tatggctttc ttcgccaatt tgaaggacag
    12541 cagctgcttg tattaacaaa tttctatgat aaagatacaa ggatcgccat cccagaagga
    12601 ttcacagaag ccaatattct tatcgataat tattccgata ctgttcttca tcaaagtctg
    12661 gagctaaaac cataccaggc aattgctttg ctgaaaaatt aaaacaaaaa cacaaaacgt
    12721 cgttcctacg agctattctc gtaagatcga cgttttgtgt tttatttaaa ataatcttct
    12781 tcagaaaatg aagagttttc atactctcct ttatcgccat aaaatttatt gtagcgttgt
    12841 ttgaatttac ggaaaataaa agtaacgagt aatttgacta acgtatagat aggaatacca
    12901 aaaataacgc ccacaattcc cattagatca cccatgacaa gtaaaacgat taagatcgta
    12961 atcggatgga tctgtaaacg atcacccatt actcgaggaa tcaccaagtc accgtggaga
    13021 agttgtacgg caaaccagac aatgacaaat ttgacagcca taaaagtaga gtcttggaat
    13081 gcaatgatca aacctggaaa gaaggcgata aacggcccaa tatatggaat gatacataac
    13141 aacccagcag aaatagctaa aacactagca aattctaatc cgatcagtaa gaaacaaacc
    13201 cagtagactg ctccaaggat aaatgaagca atgatttgcc cctttagaaa tgaacccaat
    13261 tgttgattag caatctttgt caaattttta aaatctgtac gaaatgctgg tgggacaatc
    13321 gctaaaactg actgataaaa tttttgagga tctctcaaaa ggaaaaaagc gacaattgga
    13381 ccagtaaata aagtaataaa aatagctgta acagttgaaa aaatgttgct gatccgttgt
    13441 gcgccacttt cccaataatt tccaataaat ccagctatat cagacgtcac gtcgtcaata
    13501 gaagcgaaaa attgattaaa tgaatcggca aaaggtgttt tctccaaaaa ttgtcggaga
    13561 ctctcttgaa aatcttgaaa caaatctggc aaactactga tcaatgtctt tgcctgttct
    13621 tcaagaaaag gaataagcca tatgcttcca agccccaata aaaataaaat aatcacataa
    13681 acgataatga ctgacaagtt cctcgaaagg tgcttgctca aacgtttgat gactggttcc
    13741 aaaagataat acaagatcag tgcaaacaat aatgggggca agatagtagc gataatgatt
    13801 ccgacaggct tgaatataaa cgagatctga tggaaaaaat aaatgatcag cgcaagtaag
    13861 ataactattc ctaaaacata gtaagaggtt tttcctccta aaaagcggat caaacgattc
    13921 gttgtatctg tttctttttt cactcttttt cctcctttat ctataacttc attatagaca
    13981 aaagcgggta taaaaggaaa atgatagagg ttgattgcaa aaatatcgtc aatcattcat
    14041 ataaactttc tcatcaatta aaataagaaa acgcttgaca gttcttttaa ccatatgata
    14101 aattaaatcc tgttgctaaa acgtaactat tacggtttgc gaaaagcgaa tcggaacatt
    14161 caaatgaaag aagatgagaa gaggagtgtc ataaatgaaa cgattgatag gtatattggc
    14221 catgctggtt ttagcaggta tgttaactgc ttgcggggca agtgggaaag ccgaggacag
    14281 taaagaaaaa ttgagcgtga tgacgacctt ttatcccatg tatgatttta caaaagcaat
    14341 cgtcggggat gaaggagaag tagagttatt gattccagca gggactgatt cccacgatta
    14401 tgaaccttct gctaaagaca tggcaaagat ccaagataca gatatctttg tttataatga
    14461 tgaaaacatg gaaacatggg tccccgcaat ccaaaaaaca ttacaagaag gcaatgtcca
    14521 tacgataaaa gcgacagaag gtatgctttt acttccggga agtgaagaag gacatgatca
    14581 tgaccatgaa cacggggaag aaggccatac acatgagcta gacccacatg tatggctagc
    14641 tccaagttta gcaatcaagc aggtagcaaa catacgtgat cagctgattg aagcttaccc
    14701 agaaaaacag gaagtatgga caaaaaatgc agcagcctat accgagaaac ttcaagcact
    14761 ccatcagctt tatcaagaaa cctttaagca agcaaaacaa agatcttttg tcacgcaaca
    14821 tgctgcattc aactatctag cgttagaata tggattgaat caagtatcga ttgctggtct
    14881 ttcttctagt gaagaaccgt ctgcggcgag aattgctgaa ctgaaacact ttgttaaaga
    14941 acatggaatc aactatattt attttgaaga aaacgccaaa gatagtattg ccagaacgtt
    15001 agcaaacgaa gcaggtgtat ctttagaagt attgaatcct ttggaaggat tgacgaatga
    15061 acaaatagaa aatggagaaa actatctttc tatcatggaa gcaaacctcg aagcgttgaa
    15121 aaaaacgaca gagacagaaa acccgctaga agatactttg acccccaaaa aagaaaagag
    15181 cgtctacaac ggttattttg aagacgagga tgtacaagat cgctctcttt cagattgggc
    15241 aggaacttgg caaacagtag atacgtatgt agaagatggg acttttgatc ctgtttttga
    15301 atataaagaa aaattgaatc aggataagac agcacaagaa tataaagaat attatacgaa
    15361 gggctatcaa acagatattg cttcaatcaa gatcagtgag aaagagatgg catttacgtt
    15421 taagaatgga acaacgaaac gttcaaatta tcgttatgta gggaaagaaa tcttgaccta
    15481 cgaggcagga aaccgtggcg taaggtatct attcgaagca gaagataagg aaagtggcgc
    15541 ttttagatat attcaattca gtgatcacac gattgcaccg gctgactccg aacattttca
    15601 tttatatgca ggaaatgaaa gtcaagaagt attgctgaaa gaaatggaga attggccaac
    15661 attttatcca gatacaatgg atggacgtga aattgcacag gaaatgcttt tgcactgata
    15721 ggcaaaaaag aaccgaaggg tttggatcgg ctggtcattt agtcggtcca aactattcgg
    15781 ttctttttat gaaatcaatt tctttaatcc acggatagag gctgggatgg tctcgccgtt
    15841 tgccaacgtg acttctctgg tttttttatt aatcgaccgc acattttgtg tattgataac
    15901 ataggattta tgacaacgga taaaattccg atgcattttc tcgatttctt tgatcttctc
    15961 atagaattgt aattgggcat gagtcaaatg gacttccagt ttatgtggcg ctcctagcga
    16021 agtagaaaaa tattggatgt ctttgatcgg taaaaatttg atctcatcat taaactctat
    16081 tttgaagata tctttttgga tttgggacat ttgaaaatct tcgaaaattt tgctcaggat
    16141 atgctcgaca ttttctttta aatcataaag atcttctttg acgatataat cagtcggttc
    16201 gatgttgctt tttaagattt tcatcgacat ttccgtatgg gaagtcacat agattatttt
    16261 tcctaaagga tctaattcac ggatcaattt accgatcgct acaccatcca tttcactgtc
    16321 ttctagttcg atatctaaaa agtaaatacc aaaagaagaa gttctgatct tggctgcatt
    16381 gagaagttct tccggatcag aggtagaaat atggacataa ctgtctaagt tttcaatcat
    16441 gattctattt tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata
    16501 taaatgggaa tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa
    16561 cgctggtttt caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc
    16621 aagataaaca agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt
    16681 ttcaactggt ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa
    16741 tgattcttct tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca
    16801 atcgcattgt ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc
    16861 agttcgattg gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg
    16921 agttttaatg acagtaaact acggatctca ggaactttca tattatccaa catacttaga
    16981 ttggccggaa aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac
    17041 cgtaatcctt ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg
    17101 taatcatgtt tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc
    17161 tgttgcatcc tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc
    17221 caataataaa ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc
    17281 aaagctaaga catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat
    17341 gtaaataata tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa
    17401 gtctctaacg atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca
    17461 atgtgaaaat actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa
    17521 tcgccgatgc ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg
    17581 taaaccaatt gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata
    17641 gcaattagag cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa
    17701 atcagaatga ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata
    17761 caaattgtgt aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc
    17821 cattgatttt taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat
    17881 tttgaattca gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt
    17941 taattgttta atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg
    18001 taataggaag caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt
    18061 aagaagctga gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct
    18121 ttaataatgg gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat
    18181 agcgaagcag taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag
    18241 tcaattgcag atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca
    18301 gacaaacgaa ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag
    18361 aaagaaaggt gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga
    18421 ccgctagttt tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca
    18481 ccatatcttc ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt
    18541 tatctttcat ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt
    18601 cttttgtttc ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct
    18661 tcgttttcca agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat
    18721 gacaagtttg tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg
    18781 atggagggat tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta
    18841 aatctgattt atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg
    18901 tgatgatacg tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact
    18961 ggcagttagt tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag
    19021 agcataaaga ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa
    19081 acagcttttc atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg
    19141 tttatttgaa aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg
    19201 agttactttt ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt
    19261 tatttctata aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat
    19321 aattgtgatc attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct
    19381 tgcccaagat ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc
    19441 gctattttat tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa
    19501 acgctttttg gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat
    19561 ctacgaagat agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga
    19621 agtaaagcag tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc
    19681 acaaaagaaa ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag
    19741 aatcaaaaaa agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta
    19801 ctataaagaa ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa
    19861 aatagtgatc gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat
    19921 cgtacaagaa atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat
    19981 ccgctacact acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa
    20041 ctaagcaaat tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt
    20101 gattcattga gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt
    20161 ctgagtgagg gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa
    20221 cagatgatga cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag
    20281 atagtggaaa aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt
    20341 cgttgctacc ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat
    20401 tttatcaaaa gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc
    20461 gggatgctca tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag
    20521 aacaaacaag cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt
    20581 tagaagaatc cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca
    20641 ttctccgtca aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa
    20701 agcaccatat tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag
    20761 tcctattgta cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata
    20821 tttttgtctt ccttatctta gcataaacga ttgaaaaaaa ggaaaaattt tcagaaaact
    20881 aaaatcatat cgcttcccat tttcattttt agaaaggtct gttagtataa agaaaaaata
    20941 gacaacgaaa ctttaaataa ccaacttgaa ataaacaaat tcaaagaaga aagatgggaa
    21001 ggaaatcaat cagttgaatg aaaaagaaat tctccgatta caaaaagaat tgaaacgctg
    21061 ggagaacaag tataaaaagc ttcaacaagt ctttcggcta caagaagaaa tcattgctac
    21121 ctatgaagcg gcttatggcg aagcacccgg acttattgag gaagaagtac aagtaaaaga
    21181 aagtgaagga aacagctctt tctttaaaat gatggagaaa cgttcctata atcgcttcaa
    21241 tcaagaacaa aaagcagcta tcacatatga tatgaagaaa catcttcgaa tcatagcagg
    21301 agctgggagc ggcaaaacgc aaacaatctg tgcaaaagct gtttatctga tgacgcaaaa
    21361 acaagtagac gaagaacgca tactaatgat cacttttaca aggaacgctg caaatgaact
    21421 aaaaaaacga gtagataatt tcagccagag aaaaaccgct gtccatatcg gaacattcca
    21481 tagtatcttt tttaggatat acaatgaaat ttgtcgaaaa tttcctgaag tagcaatgca
    21541 aggaatacaa ggagactttt caaaggatac tgcacaaaaa gtgaatgctg ttttgcaaca
    21601 gctgatacgt aaatataatc tttatatatt tgatcaatat ggagaaaaaa cgattgcttc
    21661 tcggttagac tattggcaga atatgaacct ttcagtagaa gaaatggttc agttagttaa
    21721 agaaaaatat gattccattg ataagaatgc acgacagccg atatcagaac gactgtatgg
    21781 attgctcacg gaactccaag aacaaaaaag aagccagcaa ttgctagagt tcaatgatgt
    21841 actccaaaat ctgaaaagcg ctttggagaa tgaggagatc cagcattata tcggccaaaa
    21901 atatgattat ctattcattg atgaatttca agatactaat ccacttcaat gggaaatcgt
    21961 gaaacggatg acaaaggaaa acaggatcaa gttgattgtt gttggagatg atgatcagag
    22021 tatctatgga ttcagaggat cagagccagc atacatcaag aactttgaaa aagaatatcc
    22081 taccaaaact cttttcttat taactaatta tcgttctcgg gcagctattg tccaaggagc
    22141 aaatagattg atcagctata ataaaaatga tcggatacca aaggcgatga ttccagcaca
    22201 aaaagaagca ggtgttatgg aagcctattt gttttctgat actaaagcag aaagtcagtg
    22261 gctaatcaaa caaattcaga catttattca aaaaaatggg aaatataagg aatcaatcat
    22321 attataccga tcagcttcac aaacgcagca gcttgtccaa tctctgctga aaacgaatat
    22381 tccttttgta ctggaagcag atagtccata tgaaggtatc tttggtatca agtctttcca
    22441 acatttctat caaaaaattg ttcattggca aacagcactg aatctgcaaa agaaaaatca
    22501 ggcctaccag caattgctcc ggcagctgat ggcagactgc tacctgaaaa aaagtgaatg
    22561 cgatcaatac ttttctccta aatatcaaaa tatagccgtt gcagattata ttttgtcgat
    22621 aagccccaat ttaaaatcga aatctgcaga aatccgagag tttgaggaag cgttgcgtaa
    22681 aacagaccag catcaagaaa tctattcgct tgttcaaagc tatcttcatc tgccacgtat
    22741 agctaaggaa atcgaccaga gtgatcaaga atggatacga gaagaagtaa agcagcatgc
    22801 tactttcaga agactccaag ccttatcgca agaaacccaa gcgacagcta aagaattaaa
    22861 aacccgcttg attgcttaca gacaaggaaa acttgatgca ctatgtatcc agagcattca
    22921 caaaagtaaa ggtcttagtt accgaaatgt ctttttgatt ggttgtaacg aaggaagttt
    22981 gccttataac ggagcgacag aaaagaaagt aatcgacttt aatgaaataa aagcagaacc
    23041 agtgacgacg atagaagaag aacgaagact attttatgtt gcgatgacta gagcaagaaa
    23101 tcgtctgtat ctatgtgtac ctcaaatgaa aaattcaaga aaattgaaag tatcgagatt
    23161 tatcaaagaa accgggagtc gtgtgaaaaa aggaaagtga tagaagagag ggtgtgacag
    23221 aagtggtcag tttcaagaaa gaagaaggaa tgtacgaaaa ttgcttctca aatttttgtg
    23281 aaatttcagc ttattttcga agaagctatt tctggaacac agtttattcg gaaaagggag
    23341 gtgtgacaag aattttgtc
//
LOCUS       DACECZ010000033        22156 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.033, whole genome shotgun sequence.
ACCESSION   DACECZ010000033 DACECZ010000000
VERSION     DACECZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.033"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(119..637)
                     /locus_tag="IWO35_001207"
     CDS             complement(119..637)
                     /locus_tag="IWO35_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0316275.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(639..1256)
                     /locus_tag="IWO35_001208"
     CDS             complement(639..1256)
                     /locus_tag="IWO35_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0316276.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1253..2107)
                     /locus_tag="IWO35_001209"
     CDS             complement(1253..2107)
                     /locus_tag="IWO35_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0316277.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2104..3015)
                     /locus_tag="IWO35_001210"
     CDS             complement(2104..3015)
                     /locus_tag="IWO35_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0316278.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3056..4390)
                     /locus_tag="IWO35_001211"
     CDS             complement(3056..4390)
                     /locus_tag="IWO35_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0316279.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTYTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4598..5428
                     /locus_tag="IWO35_001212"
     CDS             4598..5428
                     /locus_tag="IWO35_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ0316280.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5672..5921)
                     /locus_tag="IWO35_001213"
                     /pseudo
     CDS             complement(5672..5921)
                     /locus_tag="IWO35_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6112..6306)
                     /locus_tag="IWO35_001214"
     CDS             complement(6112..6306)
                     /locus_tag="IWO35_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316281.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSGF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6811..6996)
                     /locus_tag="IWO35_001215"
     CDS             complement(6811..6996)
                     /locus_tag="IWO35_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316282.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(7846..8187)
                     /locus_tag="IWO35_001216"
     CDS             complement(7846..8187)
                     /locus_tag="IWO35_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0316283.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(8187..8417)
                     /locus_tag="IWO35_001217"
     CDS             complement(8187..8417)
                     /locus_tag="IWO35_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0316284.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            8704..9042
                     /locus_tag="IWO35_001218"
     CDS             8704..9042
                     /locus_tag="IWO35_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316285.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(9247..9822)
                     /locus_tag="IWO35_001219"
     CDS             complement(9247..9822)
                     /locus_tag="IWO35_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ0316286.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(10431..11012)
                     /locus_tag="IWO35_001220"
     CDS             complement(10431..11012)
                     /locus_tag="IWO35_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0316287.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(11195..11821)
                     /locus_tag="IWO35_001221"
     CDS             complement(11195..11821)
                     /locus_tag="IWO35_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0316288.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LIESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(11843..13174)
                     /locus_tag="IWO35_001222"
     CDS             complement(11843..13174)
                     /locus_tag="IWO35_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ0316289.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(13329..13730)
                     /locus_tag="IWO35_001223"
     CDS             complement(13329..13730)
                     /locus_tag="IWO35_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0316290.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(13816..14022)
                     /locus_tag="IWO35_001224"
     CDS             complement(13816..14022)
                     /locus_tag="IWO35_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316291.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(14117..15619)
                     /locus_tag="IWO35_001225"
     CDS             complement(14117..15619)
                     /locus_tag="IWO35_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ0316292.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(15637..16092)
                     /gene="argR"
                     /locus_tag="IWO35_001226"
     CDS             complement(15637..16092)
                     /gene="argR"
                     /locus_tag="IWO35_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0316293.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(16190..16807)
                     /locus_tag="IWO35_001227"
     CDS             complement(16190..16807)
                     /locus_tag="IWO35_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316294.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            17028..18401
                     /locus_tag="IWO35_001228"
     CDS             17028..18401
                     /locus_tag="IWO35_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0316295.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            18476..19588
                     /locus_tag="IWO35_001229"
     CDS             18476..19588
                     /locus_tag="IWO35_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ0316296.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(19660..21297)
                     /locus_tag="IWO35_001230"
     CDS             complement(19660..21297)
                     /locus_tag="IWO35_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ0316297.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLLDEAKESLENLIQKLN"
     gene            complement(21465..22073)
                     /locus_tag="IWO35_001231"
     CDS             complement(21465..22073)
                     /locus_tag="IWO35_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0316298.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         6464 a         4510 c         3863 g         7319 t
ORIGIN      
        1 tcatttaaca aaacttaagt tattaagaca aatgaataaa caactacgct ttcctcgaaa
       61 caaaaaaata aagcacaaat gcgcatttac gcactcatgc tttattcgta caattaattc
      121 agataacggc agcgcatagg atgccaataa cacttcctaa aatagctcct cctaaaatgt
      181 cagtaggata atgtaaaagt aaaaaaatcc tcgaataagc gatcaaacaa gcaaataaaa
      241 aggctaatat tcctgcaact ggacttaatt cgaacaacgc agctactgat aaaaaggcta
      301 gcgctgtatg tcctgaagga aaagaagaag tcttcggttt acttgtgtaa aggacaagcc
      361 gacgattttt ttcataaggt cttttccgtc ttgtcgtttt tttgataaaa agcaaaccaa
      421 caaaaaaata gatcaaactg ccgataagaa tggctagacc atattctcta gtatcaggta
      481 tcaagactaa aacagcagca aggagtaacc aaataaaacc accattagag atattactga
      541 taaatttcaa aaaattgatt tttcgactat ccgttttccg ttgcttggcg aacttgttga
      601 taatactatc atcgatttta gcaatcaaac gcaacatgtt attcctcctt tttaagaacc
      661 ttttcttgcg tacgatggat aaaaagcagg acttgaaaat aaaacaagaa actgaaacct
      721 aataaagcaa aatagattgg tcctgcaaac cagaatgcga caagaaggat caacgtgcct
      781 gccaaataga aaagcgcgct tttgaaatca ttaaaaaaca agaaaaatgc agtaatccaa
      841 gaagcttctt tttgttccag ttgaagcaag attttgtaac taaagagaag cgcgcttaat
      901 agaagcaaaa acaacgtagt atacatcaat gtatagccaa ttcccccacc ccaatagcgg
      961 aaaatctgat aatcgatcaa tagaagaaga aaataaacac ttgctaaact atcttgcttt
     1021 ccataaagag agaagttctc acgaaacttt ttccagaata gtgaaccgag ctttttctct
     1081 tcattttttt tgtaatgata aatcatatac agaactgttc gacttgcagt tcctaatgtg
     1141 aaaacaggca agacgcacag gaaccatagt agctgtaaat ggataagacg tggaatccat
     1201 tcaatgattt ctaagaaata atcattggct tttttaccaa tcgatcgatt catcatcctt
     1261 ttgaacctcc tgtcaaatcc atcccttcaa tgaaataacg ttgtgcaaaa aagaacagca
     1321 aaatcgtcgg gatcaagaca agtgttgctc ctgccatcag ataattccat tgtgtcgttg
     1381 cttggttctg aaaaacttgc aagccaatct gtaacgtgta tttttcttga tcttggatat
     1441 ataacagagg acctaagaaa tcattccaag ctgcgttgaa ggaattgatc gcaatggtaa
     1501 tcaaggcagg tttcgtcaat ggaagcatca aatgactcca aatatacaaa tgattcgctc
     1561 cgtcgatttc agcagcttcg atcaattcat tattgatcga taagtaaaat tgacgcatca
     1621 gaaatatatt gaacgcattg ccgaagaacg atggaacgat caggggcaaa taagtaccta
     1681 cccagccgat cttggagaat aatacatatt gagggatcat cgttacaaac cccgggatca
     1741 tcatcgtaga aagaaccaaa gcaaacatca gctttttccc aggaaaatca agcttcgcaa
     1801 atccataagc aataaaagaa tttgaaagga cattaccgat cactactagc acagtgatga
     1861 ataatgtatt tcttgcatag cgccaaaaag gaaaggcggc aatcgtatcc agatagtttt
     1921 cccatacgat tttttctggg aaaaaggtcg gaggataacg aacgatttct tccattggtt
     1981 tcaatgaagt aaacaccatc caaagcaatg gaatgatcaa aatgattcct attactgtca
     2041 tgaggacata acttagtatt ttttttgttt gccgattcac actggtcttt ttgaaataat
     2101 ccatcatttg ccgtcacctc cagaataata gacccactta ggtgcaagct tcaaattgac
     2161 aaacgtcaga accaacacga tcagaaacag gatcattgac atagccatcg cataacccat
     2221 atcaaaagct acgaacgcct tgttccacat atgcagatta taaaaaagca aagaatttgc
     2281 aggtccgcct gttccatttt tcgtcatgac ataagcttct tgaaaaattt ggaaagaacc
     2341 gatcgtagag gtgatcacat cgtaaaaaat aataggagtg attaatggca aagtaatact
     2401 tttgaacttt ccccaaacgc ctgctccatc gatttctgct gattcatata aagatctagg
     2461 aacattttgt aatgtagcta agtaaagtag cattgctcct ccagaactcc atattttcat
     2521 caaaatcaac gcaggtttcg tccattgtgg atcaaacagc caagctggtc cagggatatg
     2581 gaaccagccc agaaatgtgt tgatcaatcc tgcagatgga gaaagcagct gcatccataa
     2641 gatgtagact gctactcctg ataagactgc cggtaaatag aaagctgtac ggaatacact
     2701 gattcctttg attttttgat tgagtactgt ggctaaaaac acactgaaaa tcgtcgtcag
     2761 cggtacattg aagatgacga aataaacggt gttgtataac gatttccaaa ataaatcatc
     2821 gtgaacgaac attcttttga aatttgctag accaatgaaa ttcatcttgg atgtcaaatt
     2881 ataatcggtg aaactaccaa taactgaaaa aagcataggc cctagcataa ataccaagaa
     2941 cccgataata aaaggcgaga taaataaata ggcccatctt gcttcttttt tctttcggat
     3001 agataaacgc ttcaaatttt cttgcctcct ctgtacctag acagtcaaaa atggattatt
     3061 gattttgctt gactaagttt tctactgctt tttgtgcatc tgccaatcct tcttttggtg
     3121 attttgttcc taaaagaatc tggtcaatct gttcattcac taatgtcgtg taatccggag
     3181 catctaaagg aactggtgtg atcaccgtat ttttaaagtt ttcgtccgcc atttgataga
     3241 tcatttttcc tttatccgtt aggttgtcat tattcactaa attctcattg gctgtcttgc
     3301 tcgccatgat atcaaaactt ttttcgccga atttttcttg tacttctgga gaagaaagat
     3361 atttgatgaa ttcataagag gcctcaggat cttttgctcc gtatggtact tccaagacga
     3421 atccgccacc ccacgaccaa tggcctgaac cttcttcttt ttctgggagc tgcatcacgc
     3481 caaaatcaaa atcatccggt gcattttcca tcaaactaga gtaataattg atattttggg
     3541 cacgcatggc tactaatccg gaaataaatg gatcagatac accagaccca aattcggctt
     3601 ctaatttatt gatcgtattt ttcccataat aatcttgcca gtcttggatc cactccaagg
     3661 cttccacttt atttttagta tcaatcttga ctttttcatc tttgtcaaac caactagtcc
     3721 cgttatctgc gtttagcgcc catacatcag cccctaaatt ccataatgga taaaaaccga
     3781 tgcgtgtaaa ttcttttcct tcttttttat ctagtttacg tgctgtttct tctaattcat
     3841 cccaagttgt cggtagatct tgttcactga ttccagcttc tttaaataac tttttattat
     3901 aaaaaatgac ttgtgtatat gtattgaatg ggatcgcata aggatcacct tcatattcta
     3961 ctgtatccca aagctgcgga taaaaatcct tagaagtgtc tttttctaca tatttggaga
     4021 gattagttgc ttgctgtgct tctgctcttt gggcaacgga atttatgtcc tgtacaataa
     4081 catcgggtgc atttccagct gttattgcag ataaagattt agtccagatg tcaccccatg
     4141 gctgatactt ataatttaca tggatctcgt tttgtgattg attgaaatcc tcaatgattt
     4201 cttcgatagc ttcttttcgt gcaccggatc cccaaaagga ccagaaatca atattttttc
     4261 ttccatcact tgttttttca gaagaatttg ctgcacttga actggcatcg cttgcattac
     4321 ttgatccgca tcctcctaat aaaaacaatg ctgaacttgc cagcaaccct gtaaacaata
     4381 attttttcat attttacctc caaattttaa tgtaaccgat gtcggtttta atataacgat
     4441 atcattttgt tctgaaaata acaatttata aggtatgatt ctaaaaaaat tattaatgat
     4501 accaatactt tatataattc gcaagcgttt tctttgaatt ttttaatctt tcgtagcaga
     4561 atgagaatgt attaaaaata gattaggagg aattttgatg tttagacacc agaaagaatt
     4621 gcagtttgaa gcaaaaccag atcgtccgaa tccattaatc gcgaaatatc tgcaggagct
     4681 gatcggcggc caatatggcg agatgagtgt agctatgcag tacctgttcc aaggctggag
     4741 ctgccgagga gaagaaaaat ataaagatat gctgatggac attgcaacag aagaaatcgg
     4801 ccacgtggaa atgttggcga caatggttgc tcgtttatta gagggtgcgc ctgttgaaga
     4861 tcaagaagca gcgatgcaaa aagatccaac aatcggtgcc attatctctg gaatgaatcc
     4921 acagcacgcg atcgtagctg gcttaggacc tcgtcctgct gacagcgtgg gcaatccatg
     4981 gagcggtgcc tatatcattg cgagtggcaa cttgttagca gactttagag ctaatttaaa
     5041 tgccgaatca caaggaagac ttcaagtagc acgtatttac gaaatgatcg atgatcgtgg
     5101 cgtaaaagat atgctgtctg ttctccttgc aagagatagc tatcatcaaa atctatggga
     5161 atgtgcagtg aaagaattag aagaaaaaga aggttcaatc ttagtcccaa gtactttccc
     5221 gcgtgacaaa gaacgaaaag acattgctta tgacttctat aacttctcag agggagacca
     5281 aagtagtgaa ggttcttggg ctagtggcaa agcactggat ggcgaaggcg aatatcaatg
     5341 gttgaaagaa cctaagatcg aaggcgctgc accaaaatta gaaccagtaa caccaaaaat
     5401 gtatgggaca cctccattca agaaataaat atctacccgt agaaacaccc gttttttgtt
     5461 ccacacgctt tgtgagcaaa actgactaaa gtcattaatc ataaccaccc ttcaaaaggg
     5521 tggttttctc tagagctata agctcaaatc accggccagc gcctaaagac gctggctttc
     5581 atattttatg ttcaagccat attgcctttg ccagttttgc ccacctctta aaaggtttat
     5641 gtactactcc ttagccctta aaaggatcgt cttactctcg tacactcagc ttatctaacg
     5701 cactatcatg tttttcttgt tcttgaatgt actttttgat ggtcgcttca ttttgtccta
     5761 cttttgtcac atagtatcct tccacccaaa agtgtcgata cccaaattta tattttagat
     5821 ttgcacaatc tcttgatgat ctcctgaata ttggctcgat attgattata aattattttt
     5881 cttctatact ttggtgtgaa tacaatgtgg tatttacaca tctactttgt acgggctaaa
     5941 ctatttccct ttattgctcc acccgcatag caagtggttt tgtatttcaa tcgctcggcg
     6001 cgctcaatta ggtctaaaga caataaatct aaaaacccgc tgcttcactg acagaaacaa
     6061 cgggttttca gaaaaaaatg aaatgaaaaa gatctcttac ggcaatctat tttatttttt
     6121 tgatttttta taaatggaaa atgcttcaag aagtccaaga aataaagccg tataaaaacc
     6181 agaacctatg aaatctttat ttattagata ttcaacagaa atcccgataa cactagccaa
     6241 caatatattt atcagaagtg taaaataagg atgcttttta tagaatagaa ctagtgttgt
     6301 tttcatagac tctcctttct tcatatttcc aattaatcac aaattcacgg cattactaga
     6361 aaaattaaag gggcgcagaa gtggaaaaat actcttcgac tttccctaca aaccaggcca
     6421 tcttgtgttt aataactata ccaaatctaa ggtacctcta aatctaaatt ccatttgtgg
     6481 cggtgacctc caatttttaa ctcttctatc tcccatccag tatacataac cttgatactt
     6541 cgaccactta tatatcgaac acacaaatac ctatccattg caggagtact acctaactta
     6601 gttgctactt gcatctaaat aacagctgaa aagataggac ttactcttag taaagtatct
     6661 gaactattaa tttttaaact atcattttta tcctcaacta catgcacatc tgttgcaact
     6721 tcactttctg tctcatccag ataaatgatt aaggaatcgg gaattgcaat atcttcagct
     6781 gacgctggaa tagttgagct aaattagtca tcatatcttt attataaaat aaccaacagc
     6841 aaaaaataca atgaaaaaca aacttggaac taatgtatca ttaataaatg ttgaaaaatt
     6901 cagcaactca ttactgtatg attttagcag cccaaccaac atatacccta taaaagctaa
     6961 taagatggtt aggaatcctt tagaattttt actcattcat agacacttcc ttaaataact
     7021 tttattatag aaggtattgt aaataataat attgacaaaa agtgactcaa aataaaaaaa
     7081 accttacatt atactggaat gaactgtatt gatagtgtac ctaaaagctc tactgacata
     7141 agatcgtgta atcggtaaag taattttatt aattatacgg gatgtaatca attagagaat
     7201 aaactaatca taaaaggtac ttttacattc ataattgtcg ctttgagatt ctactttggt
     7261 tcactatctg acccaattgc acattttcga tcattacaag tattggtgtc gttagaacaa
     7321 ctaaataaaa actagcggta gatttttcag aatctatgct aatttctgta tggatgttcc
     7381 agtgatggca aatttctctt ttcattgttt ttcttgtcgc atgatatgcc tttttgtgtt
     7441 attcagttaa actatagaaa ttctttccta gacagatctt gatcccaaat tttacgtttt
     7501 tgagcatcga tatagtttaa aatgacatta ctcatatcta aagagtttag attttttagg
     7561 actaaaactg tcagttgttc tttttcttct ttctgttaaa gtattgtcgc gacttttcgt
     7621 atacgttaag atatgtcgtg aaaaaacaag ttttgtgaca caaaaaaacg aatgacttga
     7681 ggaaatcacc tcaatcattc gtttaaaatg ctatatattc tttagtttaa gatacggaaa
     7741 ttaatgccat cgaccacact agtagaatga acgacattta cttggccttt ttttaatttc
     7801 gttatgttta gcatataaaa atattataga gtttacttac aatgcttaaa acatatactg
     7861 tataatctga tcaattttag ccatgtcttg caatggtaag ttttctattt ttttgagttt
     7921 tcgttcctta aaatcaagag attttaattg tgtaataatt acttgtcctt ttatatctag
     7981 ctcttcaggt aatgagtaac gtgtcggtaa attctttata gtggaagtaa ttggacatat
     8041 gaccgaaaac attgttttac ggttaaattc ataacgagag acgactagtc ctggtcgtct
     8101 tttttgaatt tcttttccta cggagggatc aaaatcaatc catacaatat cacctttttt
     8161 aggaatataa attctttctt ctgacactat aaaatttctc tcccttcagg agttaaatct
     8221 tcccattcgt ctttctcata atactcggcc tcttccccac cactaaatgg atcttcaatt
     8281 tttggaacta acgtaattgt tccatcatca gaatacacaa cgatatattc ttggttttct
     8341 gaaggtttct ttccgttatc agaaggtaaa gttaccacta cagaacttcc ttgtaatcta
     8401 gattttgtaa caagcattat catcactccc ttaataaaag tatactatat aacactaagt
     8461 aacgcaaaat tcccctttgt aaactatcta gaaaattgtt ttgtttaaag tatatcttat
     8521 tggatataaa tagaaataaa ttgtagccag ttcatttttt ctttaactaa aaaaataatc
     8581 caagaaacat tgaacctatg gatccaaaag ctacccaaat actatagcta accccccgct
     8641 accttcaatt ttgcagataa aacgctttgg gagaaaattt caaagacaac aggccatcct
     8701 atcatgcccg attataaaca aaaagctggc tattcctttt gctctgacaa atttcaattg
     8761 ggaaagttca cgaaactagc aggtgaagaa gtttcaactg ttcggatcaa agaatgcccg
     8821 cttcaaatag aaacattggt tcaacgaatt tcaactagac aagactttgc tattgtagaa
     8881 cgtgagatcg tagctatttt tgtagaagaa gggattttac atgatccaac tcatatcaat
     8941 gtggataaat ggcaacctct tatctataaa ttcagagaat atacaactgc caaccagtcg
     9001 ttaactttga attttagatt ccaagaattc aaagagtcat aatgataaac atcgttaggt
     9061 ataaataaaa aagactcgca gcacgagtaa aaaacaaata gaagctatac attagaaagg
     9121 tcgactgtga ccctatagat gcagtatatg cagcttatga gcatttcttc tgttctcttt
     9181 gtttttggga tctgtgctga agtctttttc gttttttgcc gctctttctt tgaaaaatcg
     9241 attatgttat accttctcca ttactaattc aggcatctgc tgagtaatga tttttcgcgc
     9301 aaaagaaata tctgttaccc aaggttcgat ttgttcttca gtcaagacaa gcggcattcg
     9361 gtcatggatt ggtgcgactg tgcggttagg ttcagtagtc attatgattg attctgccat
     9421 tttttcatcc tgatggctac gataaaaacc gccaatatac actatttttt ccttatttga
     9481 aaacataaat ttgtcttttt cttcattcca ctcataaaag ccactcattg ggaaaacaca
     9541 acgattttgc aaaaatggtt gatgaaaggt ctttttttct tctactgtct ctgcacgggc
     9601 attaatcatc aattgtcctt ttttaaatcc ggtaaatccc cacttcatga tttccggagt
     9661 gaggatgttt tcttcctctt ttcttaacgt cacaacaaga tttgacggaa aaacttcccc
     9721 agctgctaat tctgctgcct gcttcggttg tttcgtctgg gcttctcgat aataattctg
     9781 aaggagctct gactctagat caaaaaaata acgaccacac atcctaatcc ctcccaatct
     9841 ttttttctat catacactgc aacaagaagt tcgcaccaac aaaacgcttt caatttttgt
     9901 agaactaaaa aagttgtgac aagtgacaaa ctctccagtg aatggtattc tcctgccaac
     9961 tcctcggtca caagtcacaa tattcaacaa acatgagaag ctgctttttg aaattgttcc
    10021 ttgtgactcg gagtgaaacg gcaggcttac gacctctggg taaacggtgt tcaaaagctt
    10081 gcatctgcgg taataagccc aaccaaacga aaaatatgag cagctatttt tatttggctg
    10141 ttcttattac ttgatgcagg gcagcttttt cacaacctct ccactaaacg gtgttcaatc
    10201 agctaacctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg
    10261 tttctctaat actcaggtca aagcgctgat ttcacaacct ctaatacacg gtgttccaga
    10321 agtagcttct tcgaaaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa
    10381 attccttctt atttcttgaa gctgaccact tctgtcacaa cctctaatta ttaaagaacc
    10441 ttttgcgaag ttaacaatct ttctttgatc aattgaaaat tctgcctaag tagtgataga
    10501 tcctgtgtga ctttcaacaa taagatactt ccctggattt cagcaatcaa cgctttggct
    10561 tgacggtctg cttcttcatc ggacagtttt tcttcagaaa gtgccaggat attttgtttc
    10621 gtatacttga cccattgatt gactaattct agtagcaaca gacggtattc ttcatccact
    10681 gtactcaact caataatcaa attacctaca ggacagccat gataagtgat ctgtgtctca
    10741 tgatcagcga aaatccagtc caacatttct tctaatgctt cttctggctg ttttccagca
    10801 tttaagatgt cttcgaacaa ttctgtctgc catacgtcca tatgcgcatg gatcaccgct
    10861 agacaaactt ctttttttga agaaaagtaa taataaaatt gaccttttcc tgtatcagaa
    10921 gctgctagaa tatctcggat tgacgtttca tgatagcctt ttttataaat aagatctttg
    10981 gtcgcttcca agatcttttc ctttgttttc aatgatattc tcctttttca tttctcatag
    11041 tcctatatta tacagtaaat tcatcatttt ttcttcttaa tcgtttaaaa aagactgtgc
    11101 caaattatct gacacagccc tcactataaa actgttctca tatagctaga tgttcattga
    11161 ttcaagaact gcgaggacaa aagcaattcc tatcttatga tagttgctct aatacttcct
    11221 ctgttgtcat cacttgatct gcaataaagc caaagttcgt caacgctgca tccattgctt
    11281 cttggccagg agcaccaatt gcatctgtcg ctaccacaac attgaatcca ctctctatca
    11341 attcacgcat atgggaatcg ctacacaagt ttccgttcat tccaccaaga attactgtgt
    11401 cgattccatt ttttctcaat tggagcgcca aatcattgct ttctggtcca aagattttat
    11461 gaggagagct gatgatcgta ttttcatcca aataaggttt caattcttcg ataaaatcgg
    11521 ctcctgaacc ttcttccact gattcatatt gactttttct agcaaacatc tgctgctcaa
    11581 gcatcatatg ttctcctgca ccgccaaatt gccatttatg gtcatgtggg taaaaataat
    11641 gaggagaaat aaagagtttg tatccttttt cttttgcttg ttttaccatt tcaatcatat
    11701 tttcgatcgt attgtatttt tctaagatat cttttgtcaa accaaagcca ccgcctgctg
    11761 gtttcaagaa ctcgacttgc gggtcagtga gaactactgc tgtattttta ttattgaaca
    11821 tggtggattc tccattcgtt atttatttta atacatcatt tagagcttca ctcattgtcg
    11881 gatgagaata gatttgatct ctcaataacg tataaggaag ttttccattc atagctagcg
    11941 aaatcaagtt gatgacttca tgagaatctt ccgcatagat acttgcccct agaatcaagt
    12001 cagtttcagg gtcgactaaa actttgaata cacctttagg atcttctaat acttgtgctt
    12061 tagggacacc gcttgtcata tacttgaata atttgtaatt gacgccttgt gctttggctt
    12121 gtttttctgt taaacctaca ttggataaag tcggagtgat aaatacactg taaggcacag
    12181 ctttccggtt attcgttgtt cgtttattct cgcccttcaa ctgatccata atgattcgat
    12241 aatcatctag agagatataa gtgaactgca atccgccttt gacgtctcca atcgcccaaa
    12301 tgttgtctgc atctgtacga aggtattcat ccacagcgat tgctccgcga tctgttagtt
    12361 taacatctgt attttctaat ccaagtgatg ctgtacttgg ttttcttcca gttgcagcca
    12421 aaacttttga cgcttggatt gttacttctt ggccatcttc tgtaaaggtg acggcagctg
    12481 ttgattcttg gtctgctact tgatctacag atacacctag atggaattcg attcccgcat
    12541 ttgtcatgtc ttctaaaatc atttgcgcga tatcttcatc ttcacgtgga agaaattcag
    12601 aatgggcatc aagaacgaca actttagatc cgtattcatt gaacattgat gcaaattcca
    12661 agccaatata tccagccccg atgatcacta atttttccgg taattgttcc tgatccatcg
    12721 cttgcgtaga atcaatcaag tagcgacttg tattcaatcc tttgattggt aaaatgattg
    12781 gttcagaacc agtattgatg aaaatccgtt cgccttcaac aatcactttt tcaccattat
    12841 tcgtcacttc gatttgatgg ttagaaagaa aacgtgctgt accgtccatc actgtcccag
    12901 ttgcctcatc gctgatcata tgataatttt tggcgcgaag catgcccgtc aatttttctt
    12961 tccgagcaac agcctctgta aagtcgattc ctctttctcc attgaaaatc aatgatttag
    13021 aaggaataca gccgatgttg atacatgttc cgccatacat acgtgttgac ttttcaacga
    13081 ctaaaaccga ttcacctttt gaagctaacg ccttcgctaa agtcttaccg ccctttccaa
    13141 aaccgatcac aatattttga tatttttcca tcatttttcc ctcgcttctt ttttacctac
    13201 cgattggtac aaaaatatga taaccgataa ttgtctattc tccaaatcat ctgctttatt
    13261 tgtcaaaaaa aaccagacaa aaagcggtcc tttcacgttt ttagtctggt tcgatttcct
    13321 ttattcttct aaacaatcgt tacttctaca tcgatatttc ctctagttgc tttggagtaa
    13381 gggcagaatt catgcgcatc ttctgcaatt ttttgggctt cttcttcgct gatgccatcg
    13441 atatgcactt ctaatactac accgatctga tagccgccat catctaaatt ataaagagag
    13501 acacgtgctt ttactgtgga ctctgctgtc attcctgctt ctttgatcat atgttgtacc
    13561 gccccattga aacaagaagc ataacctgct gcaaataatt gttctggatt tgttccttct
    13621 gcatggattc ctggaggagt gattttcata tgcatcgagc catttggtgc ctcgacttct
    13681 ccattgcgcc cgccatggtt gatcattgct gtttcgtatg cttttttcat tgtactgctc
    13741 ctttcgattg accatttgct acttggctat aattatagtg tatgacaagt aaggcgcttt
    13801 ctacaaaaat tctgcttatt cgaataccag aatgccgtct tctactattt ttccaaaaaa
    13861 caaaaaaata ctactcgtga acaacagcgt catatttgtt ccaaaataaa tagcaatctt
    13921 ttctacataa tcttttatat gattgatttt aagatacata tataccagaa agttgacccc
    13981 ataaaccaat actgttaaaa aaataaccat attcgttacc attttttcct ccttttgctg
    14041 gtctccctac cagcactcga tttaaccata ccactttttt tgccgtccaa gcaactagcc
    14101 tttcaaaaag actggtttat tctgctgtta atgattcaag atcagaaaaa tgtccgaata
    14161 attttgctaa ttttactaac gccaacttgt tctttccgaa ttcatcattt tctatctgta
    14221 atactagtaa cggctcatgt aagcttaaac ggatcaaaaa ccagccgttt ccgtatggct
    14281 cttctagatc atatcgaata ccttcttcat tttccgggtc gaagttcatc cctttttctt
    14341 cttccaacag tcgcttcatt tcttctaata tcttgtttcc ttgttcgcgt ggttcatcgc
    14401 tgaacaattt taatcggatt tcttgtactt ctacgggctg tttcaactct ttgatttgtt
    14461 catctaatgt gatacctttt ctttttaatt ctggcaaaag catcaggatc tttgcgatga
    14521 catatgcacc atcatccaga aaatagtttt ccttgaatgc agcgtgtccg cttgtttcga
    14581 tggctagctg tgtatctttc ccaagacggt taagttctaa tgctttattg attacatttc
    14641 gatagccgga aatataacga acttggatgc ctcctttttc ttcaatgaat ctttgtagat
    14701 gagaagaagt aggggaattc gtcacgattc ttgttcctgg atgctctttt aatacgattg
    14761 tacttaatac tgcgatcaaa ttatttcgat tgatcactgt tccagaagca ctgacaactg
    14821 cagaacgatc cacatctgta tcgaaaatga tcccaagatc tgcttcattt gctaaaacag
    14881 cctgcttgat actttccatc gcttctttat tatcaggatt tgggatatga ttaggaaatc
    14941 ggccatctgg ttccaaaaat tgggaacctg ttgaatccgc acctaagcta ttcaatactt
    15001 tttcagcaaa gaatccacca gcaccatttc cggcatcaac aatgattttc caaccattta
    15061 gtggtttgct ttctagtgtt ccaatcccct ttttaatttt tgtgactaaa tcttgcgcat
    15121 aaggtgtcaa aaggtctgct ttttctacgc ttccagtttg ttcgcttttt attttttgct
    15181 gctgtgatag gataaaagca atatcttctt tttctgctcc tccttctgag gtaaagaatt
    15241 ttattccatt ataataatag ggaagatggc tagcagtcag cataaccgca gcatcgcacg
    15301 agaactgttc aaattgagtg gacataaaca tagcaggcgt agtagctaat tcgaaatcga
    15361 tgacatgtat cccttgttct ttcagacttt ggatcaacgc ctctttcagt gctggtccgc
    15421 ttatcctact gtcatgacca atcccaatcg tccatttttt atttggatcg gttgatttct
    15481 tctttttctt tatccagttt gctagtcccc agccgatctt ttttaccatg tctgtcgtca
    15541 agtttacttc atatccttca actggtaatg cgattcctcg aatatccgat ccattttgca
    15601 gcgctttcat atctttcata taaaagtcct cctcatttat tccctcattt tttccagctt
    15661 ttctgcaaat cgctgtgctt cttcttctga gcggcaaata ataatgaatg tatctacacc
    15721 ggccatcgtt ccgacaactt cagggtaagc cacttcatct agccaattcg ttaccacatc
    15781 ggcaccattt cgttcagtca atacgataac catgaactga acagactgga tacgcaaaac
    15841 ctcgcgtcta attgctgtac gcaaacgttc ttcatttaaa gaaacagatg gctggttaaa
    15901 taatgtatag cgtactattt tgttttcatt ttgacttttt actaacttca tttctcgaat
    15961 gtctcgagaa atcgttgctt gagtcacatc ggcgcctgct tcatgcagcc gcgcaagcag
    16021 ctcttcctgt gtcccaatag gttgttctgt aatgatctgg cggatcagtg cttgtcgctg
    16081 tttctttttc atattagaca cctctcaatg ttgattatag caaaagataa gtaatatttc
    16141 ttcatatttt ggtatttttt acttttattt tttgttttca ttcaggttgc taaccttttt
    16201 ctattttaac acgcaaaatc gtcttcattt tttcaggtgc tgattttctt ggatcgctga
    16261 tgtaaatctc tttgtgttct ttagaagctc tttcatagcc ttcttttgcg atgaatgctt
    16321 ctaatttctc aaacgttgca ggttcttcgg catagggccc gacgtgtaag atttgggcac
    16381 aaagaccttc ttccatcttt ttgaatacta cttgttcttt caagtcttcc ggaagtttgc
    16441 tagaacgctg aatggcttct tgtgctatcg cttctgtaac aaagtcaggc tgtcggatca
    16501 tcaattgata agaaaaatgt tcttttttca tcactggttc atgcaaatac ttttcttgca
    16561 gtccccattg tccttccagt ggatagacag tatacatctg ataatcagga atcagccaat
    16621 tttttttagg cgacatgcgt attgcatagc tgactgcata tagacaggct actcttcgtt
    16681 taaagtcttc ctgattcggg tcgccgatcc ctctgatgct aaagaatggt tttttaggaa
    16741 cctctagtat ttgcgggctt tttttcatac tatagctctt gtctttacgc cattcaattt
    16801 tttccatttt tcttcctccg cgaaattcca tttagttatt tattatcata cctctttttg
    16861 agtgacttgg ctaatcgtgt tgacactatt tttcagtttg tgctagaata agtgaagtaa
    16921 ataaatcgta cagataacac gtttcggcgt gtcgagagag aggcggtttc tatttttata
    16981 atagaaatca ctctcttttt ttacgaaatt ttttagaggt gatgtaaatg acaaaacaaa
    17041 aacagctgct tttagtacct gcagtaattg caacagggat gatgtctttt gcgggcgtat
    17101 tgatcgagac ggccatgaat gtaactttcc caactcttat cagacagttt gatttaacga
    17161 ctgcacaagt ccaatgggta acaacgattt atcttttgat gatttcgatc attgttccgc
    17221 tgtctactta tttgaatcgc aattttagtc tcaaaaaact ctttttagct tcaaatctgc
    17281 ttttcttggc aggtataatg atcgatttct tctctcctac cttcatggtc ctattgtttg
    17341 gtcgattgct ccaaggtgca gcgacaggta tcgctcttcc gttgatgttc catattatct
    17401 tgacatttgc ccccctcaat cgacgaggcg cgatgatggg tgtcggtact ttgacaacgg
    17461 ccattgctcc agcaattggc cccacttatg gaggcattat gacttcacat tttacatgga
    17521 atcatatctt cctgtttctt gtgcccgttt tgattctttc tttgattatc ggctggtttg
    17581 ctattcccga gatggcagta caaaaaagtg gttatttgga ttggataagc gttttaggaa
    17641 tttgtctttt gtttagtgga agtctaacat ttttgagtac gttgggaaca gtttccagct
    17701 ggattgcttt aatcatcggc ggtattggct ggttcctttt ttactatcga agtaaaaaag
    17761 tggagcatcc actgatccga ctgtctattc tttcaaataa aacattccgc ttgtttttga
    17821 ttggcttctt agtttatcag tttttattac ttggtgtatc ttttgtcttg ccgaatttcc
    17881 ttcagatcgt tcaaggaagt agtccttttg tcgctggatt agcgatgctt cccggcgcag
    17941 cggttggtgc tttgctttct cctttttccg ggaaaatgct ggatcaatat ggtccgaaga
    18001 agccaatttt tgctgggctg attctttctt tgattggttg gctagcatta ttcctactca
    18061 taggacacgc ttcagtgggt gtattgattg cttgtcatgt gttttatatg atcggtatcg
    18121 gattatctta cagcaatatg atgaccaccg gaatgaatgc actgaatgaa gaactacaag
    18181 gagacgggaa cgcaatgttc aataccttgc agcagttttc tggcgctgtg gctacttcat
    18241 tagtagcggt cattatcaat tacgtccagc atcatacttc ccataactat gaagtgtcta
    18301 ctacattagg atcaaaagca gcattaggcg tcttgttgct gttgcttctc gtaagctttg
    18361 ctcgattcgc ctattatctg ttctttgcta aaaaagcgta gattccatat acggcaggtg
    18421 ttgcatctgc cgtttttttg tgtgaaaatg gttgtttgta aaaggaggct tttttatgga
    18481 aaaaattgcc attatcggtg gcgggatcat tggtatgacc ctagccagtt acttagatac
    18541 atcaaaattc aatgttgctt tatttgatga agggacagga caggcaacta aagctagcgc
    18601 tgggattatc tccccttggt tgtcgaaacg cagaaacaaa caatggtatc gtctggctaa
    18661 agacggcgct gcttttttcc ctaaacttat aaaagatttc caacttgatc aaacgattta
    18721 cgcacaatca gggacacttt tgcttcgacc tgaaaaagat ttagctgaat tggctgttct
    18781 tgccgagaaa cgaaaagaag aagcgccaga aattggaaac atcgaatggc tttcacctga
    18841 acagacatct caagcacttc cgttattgaa accagcagtt tcactgcgta tcagtggtgg
    18901 tggtcgtctg gatggaaaag catatttaga acatcttggt cagcgtttag cacaaaaaca
    18961 aatggagcag atccaggcaa aagtcacttt gcataagaag agcgattctt ggctcatcaa
    19021 gggaccgttt ggagaataca cagctgatcg tgtaattttg acacctggcc cggcacttaa
    19081 gcctttatta caagaattag gctatgctgc agatatacgt ccgcaaaaag ggcagctctt
    19141 agcatttcag acatcttata agatgagtca agactggcct gttgctatgc ttgacggaga
    19201 agctgatttc attcctttca tggatggaaa gatcctttta ggtgctaccc atgaaaacga
    19261 tcaaggctgg gatttggctc ctactagcga agcttataag ggattgacta agagtacaga
    19321 acagttcttg actgatccag atcagataaa aaactggcca tacgagttcc gtgtaggtac
    19381 ccgtgcttat acgtcagatt tttccccatt ttttggcgta ctgccagatg atccaagctt
    19441 actggctgct agcggtctag gctcttctgg cttgaccaca ggtccttata tcggctatct
    19501 attagccaac ttcttaaata ctggtcattg gtctacggaa gattatcaaa aggacatctc
    19561 cacctatatt acgaaaaatc catcatagaa aaacagcttt ttcaagtaag gatcccgagc
    19621 gatattgcgc atggattact tcttgaaaaa gctgttattt tagttcaatt tctgaatcaa
    19681 attctccaaa ctttcttttg cttcatctag caagagatct tttcctatct gagcttcaat
    19741 gaatcgtaaa cgaatatcaa gtcgttgttt caccagattt ttataggacg tatcctcaaa
    19801 agaaacagga aaatctggta taggtaaata gtggatgtct tcaaatggac caaacgactt
    19861 gaattctgcc gtttgttgca cgattctttc aacgattgac aatgtcgttt caggtttgat
    19921 ttttttatca tcgaaatatc cagtattcag gacatagcag gctgtttgct gtttcaaaaa
    19981 caactctcga aaagcttgat agtcttccct aagaggatag acacggaaag gattagcaaa
    20041 tggttcaatg actagctcat ctagctgtct ttttgtagtg atattttccg cgtctgatcg
    20101 ttttgttgct aatgttgcac caaaagacgc tgctaaaact gggtcattga tcttgataat
    20161 tggcggaagc gaatcatctt tcatgatcca aaaaacagca tcgatctttt cgctaagatg
    20221 atcgactcgg tcgggtgtcg cataacgtga tttgactgtc cgcccatttc cgtttcgtat
    20281 atctcctgtt actaggactt tttctccttt acttgtacat gtcacgccca cattctgaca
    20341 agtaaggaaa gaagaaatgg ccggatcatc agacggatag tcttgtgttt tatcaaagta
    20401 agaaggttcc aaggcagtag caaaaccatt ttttcgagaa ataacgaaag catcgtcatg
    20461 caagatcgaa acagagtgac cttccgtatg ttttgctaag gtaatcgttg attttccaga
    20521 accggacaac ccaaatacag ccatcgtata ggtctggtca ttactagcat attgtttcat
    20581 cccaccgtgg caggcaataa agccattgcg atttcccaat gcccaagcaa gcgtcaaagt
    20641 cgcttttttt aattctccaa agtaacgtaa tcctagcaca gccgctgtat tatggagagc
    20701 atcaaacaaa atgagtccat ttggaaatgt ttcatgtgtc cattcgggat cacaaaagat
    20761 ataaagatct ccctcatcaa aggaaacaga cttttgataa gtctctaaga tcttcgaatt
    20821 cgctggctga aaattcagca aataagaata caaattgttc tcaaatcctt ctggaatcag
    20881 caaatgactt tgcacagaaa attcttcaga taggccaacg attacttttg tatggtaaaa
    20941 ctggcgcttg cgactttgat acacagcttc tcttaaaatt ttctcgtatt tgctttgttc
    21001 cttgtctaag tcctcaaaga tccgccgagc acttgctgtt cttcccacaa tgcttccatc
    21061 attgaatact agtgtcctaa catcagctgg taaatctagt tcttcagcat gtgagattgt
    21121 taaatctgtg atgatcgttc ctggactccc ttttgctagt tgataagctg tcttcctgtc
    21181 aaaaacttca tgaacattat taccgtaaaa tgtcgtttct acaatcgatc gaatactgga
    21241 taacagtgga tttccacttg tgatttcgtt taattcgaga ctctcttttg tactcatggc
    21301 tcctcctcat tccctaaaca aaaaaacgtt ttctttatta tacattattc gagtttttat
    21361 ttgtactgct gcgctgtggg aagcaaacga gttatttttc acacaaaaaa accgagaact
    21421 tgaataaaca agtttctcgg tttcatcatt ttccttttat cggcttagta ccagccgttt
    21481 gctaaccaga atgttttagc agcatcccat gaaccataac gagatgttac atattgttct
    21541 gctacacgtt cttgattggc agctgaatag tcgccgttta agtatgaagc atctaattga
    21601 taacggccga tgtaacgtcc gttagtagct gtgtatgaac cgcctgattc tttttgtgcg
    21661 atccattctt ttgcagagct tgaagttggc gctgttgcag cagctggggc agcaggagct
    21721 acttcaactg tttcaacagg tgcaacgtat gtatgttctt gcgttgctgt ttcagttgta
    21781 gatgctgtat tttctgttgt tttgttttct gttgttgctg aagaatcatt tgaagttggg
    21841 attgttaatt gttcaccaac ataaatgcgg ttgatgtttg cgatcgaatt tttttcagca
    21901 attgcatcga tcattgagtt gtctcctgca aatttttgtg agatcttaga taatgaatca
    21961 cctgatttta ctgtataaac ttcatcagcg tgagcatttg tagttcctac aaacattgca
    22021 gtagcaccag cagcaaaagt tgtacctaaa acaattttct taaatgaatt catattaaac
    22081 tccttttttt ctccgttttt tattctttcg acgctattaa ctttatcatt tagaaatatt
    22141 tcataggtaa aagaaa
//
LOCUS       DACECZ010000034        21493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.034, whole genome shotgun sequence.
ACCESSION   DACECZ010000034 DACECZ010000000
VERSION     DACECZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.034"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(289..362)
                     /locus_tag="IWO35_001232"
     tRNA            complement(289..362)
                     /locus_tag="IWO35_001232"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(326..328),aa:Ile,seq:cat)
     gene            complement(551..1570)
                     /locus_tag="IWO35_001233"
     CDS             complement(551..1570)
                     /locus_tag="IWO35_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0316299.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1581..1787)
                     /locus_tag="IWO35_001234"
     CDS             complement(1581..1787)
                     /locus_tag="IWO35_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0316300.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
     gene            complement(1920..2879)
                     /locus_tag="IWO35_001235"
     CDS             complement(1920..2879)
                     /locus_tag="IWO35_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0316301.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            3145..3489
                     /locus_tag="IWO35_001236"
     CDS             3145..3489
                     /locus_tag="IWO35_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ0316302.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            3490..3909
                     /locus_tag="IWO35_001237"
     CDS             3490..3909
                     /locus_tag="IWO35_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ0316303.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(4001..4351)
                     /locus_tag="IWO35_001238"
     CDS             complement(4001..4351)
                     /locus_tag="IWO35_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316304.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(4317..5648)
                     /locus_tag="IWO35_001239"
     CDS             complement(4317..5648)
                     /locus_tag="IWO35_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ0316305.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            5904..6470
                     /locus_tag="IWO35_001240"
     CDS             5904..6470
                     /locus_tag="IWO35_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0316306.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            6615..6702
                     /locus_tag="IWO35_001241"
     tRNA            6615..6702
                     /locus_tag="IWO35_001241"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6647..6649,aa:Ser,seq:cga)
     gene            7036..8010
                     /locus_tag="IWO35_001242"
     CDS             7036..8010
                     /locus_tag="IWO35_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ0316307.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            8335..10230
                     /gene="asnB"
                     /locus_tag="IWO35_001243"
     CDS             8335..10230
                     /gene="asnB"
                     /locus_tag="IWO35_001243"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ0316308.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(10425..10871)
                     /locus_tag="IWO35_001244"
     CDS             complement(10425..10871)
                     /locus_tag="IWO35_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0316309.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            11099..11248
                     /gene="rpmG"
                     /locus_tag="IWO35_001245"
     CDS             11099..11248
                     /gene="rpmG"
                     /locus_tag="IWO35_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0316310.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            11336..11854
                     /locus_tag="IWO35_001246"
     CDS             11336..11854
                     /locus_tag="IWO35_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0316311.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(11931..12887)
                     /locus_tag="IWO35_001247"
     CDS             complement(11931..12887)
                     /locus_tag="IWO35_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0316312.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            13053..13859
                     /locus_tag="IWO35_001248"
     CDS             13053..13859
                     /locus_tag="IWO35_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0316313.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            13856..14845
                     /locus_tag="IWO35_001249"
     CDS             13856..14845
                     /locus_tag="IWO35_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0316314.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            14842..15846
                     /locus_tag="IWO35_001250"
     CDS             14842..15846
                     /locus_tag="IWO35_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0316315.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            15978..16520
                     /locus_tag="IWO35_001251"
     CDS             15978..16520
                     /locus_tag="IWO35_001251"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ0316316.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            16540..17238
                     /locus_tag="IWO35_001252"
     CDS             16540..17238
                     /locus_tag="IWO35_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ0316317.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            17259..17471
                     /locus_tag="IWO35_001253"
     CDS             17259..17471
                     /locus_tag="IWO35_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ0316318.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            17471..18433
                     /locus_tag="IWO35_001254"
     CDS             17471..18433
                     /locus_tag="IWO35_001254"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ0316319.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            18451..18846
                     /locus_tag="IWO35_001255"
     CDS             18451..18846
                     /locus_tag="IWO35_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0316320.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            19009..19374
                     /locus_tag="IWO35_001256"
     CDS             19009..19374
                     /locus_tag="IWO35_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ0316321.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            19371..21182
                     /locus_tag="IWO35_001257"
     CDS             19371..21182
                     /locus_tag="IWO35_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ0316322.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(21244..21411)
                     /locus_tag="IWO35_001258"
     CDS             complement(21244..21411)
                     /locus_tag="IWO35_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ0316323.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
BASE COUNT         6832 a         3719 c         4325 g         6617 t
ORIGIN      
        1 atataaaaga gtttaaagcg taacacactt atggggaagt ggcttggggt gcgctttaaa
       61 ctcttcttta ttatcatctc acaatttaac ccaaatgtct ttctatttaa aaatcaaagt
      121 aaaacttttc aaatatacag aaatataact atgtgaaaca tcctttcatt aatccataaa
      181 aggatacata aaaaagccac tcatttgagt ggcaatgaag aaaagcttta gcttgtataa
      241 tactcttcaa aaaattctaa cacagaacga ttcaaatggc tacgttaatg taccctgtag
      301 gactcgaacc tacgaccgga cggttatgag ccgtctgctc tgaccaactg agctaagggt
      361 actggttgtt gccacataaa gccataaaca atcaaccagt agaatgtgtg gcaacaaacc
      421 tgttatcgca tagcttggag tgtgactatt atgggtgata gtgaagatat gcgataacat
      481 cactatttta tcgaatgatt tttatagttg tcaatatagt tatgtaatgc tcctcaacga
      541 ggagctattt ttatcgttta ggaatattta aataccaacg tttgtcatgg aaatcttgcg
      601 caccgccttt agtgtttccc tctggatcat tcgttgcccg catcatgaca tagactttct
      661 tattagggaa gttacgcatg ttaaaagata catgatagcc aacatttcca gaagtattat
      721 aagcttgatt tacatctgat ctataaattc catcagctct tactcgagct aattctttcc
      781 cagtattgta atccataatg aagatatact cgtatttata gttagcaatg tgccatccag
      841 ctacatgcaa atttgcattt tcgatttctc caaactgatc aatgtgggcg taattcgttc
      901 catctgtcag tgtaggatta gctgcaccag ctcgtgttgg atcaatgaca ggcttgtttt
      961 cagaagttgt tggattttca tcggtaaatc catgagctaa atcatatgct aatttttctt
     1021 tacttacgcc catttcagaa agataaccgt aaggatctgt atgatcgccc cagatgtttt
     1081 gtgttaccca taaatgcgat ttgattcccg gttggttata aggagtgtcc aacgttaatg
     1141 gaataccata tttcattgct gaatctctag ccaattcaac gtatgccttg tagtttttct
     1201 caaacgttgc tttatcatgt gtgtgttgta actcaatctg cacaggactg ttggcattag
     1261 catacgaacc agcaccgtac tgtacataac caggttgacc gacttgataa acaattccgc
     1321 cgtctcccac aatataagca gtataagcgc tagtccatga acgttgcata tactgcgctt
     1381 cattgcgtcc tgttgctgtt tcattagccg tttcatgcag taaaatatac tgattatttg
     1441 ctacttgtga gctaccttca tttgcgccca aattaaattc attgttgata gtataggcaa
     1501 atccgttaat tggcaataaa aaaagagccg ttaacaggct catcgcagta atagtaattt
     1561 tctttttcat atctatcctc ctatttatct gatgaaattt tcttatacgt tcgatttgat
     1621 acacctaaaa tactccctag aaatgctcca aaacctgtaa taatcatcac cgttatatct
     1681 gtgtaccgcc agttaacacc ttttccgaca agaccgacaa aagtcgctaa agcggggata
     1741 actactatag cgatccattt caaaatatca aaggttctat tgttcatact atccacctcc
     1801 cttcattggt tgggctactt tctttttcaa tcgcttgttt tcttctatca atatttctct
     1861 ttctgaaagc aaggcatcaa tttgcccgtc cagttgttca attttatctt tcttaattgt
     1921 caaattaagt tagacagatc atcaatactt acgtaactca aaaatacttc caaaggtgtt
     1981 cgataatcca gtgattttct aggaatatta tttcgtttag atgcgacaga ttgaataaaa
     2041 aattcatcta ctgaattgaa atccatagat ttcaataaac catctctacg taacaatccg
     2101 ttagaattct cgtttaggcc tctttgagac ggcgttcctg gatcagcgaa ataaatggca
     2161 atatcattga cattactgat ctgtttccaa tttgaaaatt cctttccaca atcaaaagtg
     2221 atggatttga ataggttttt cggtacagat tcaaaccatt gatttaattt tttttcaata
     2281 tcaatcgctt gtctaccaca cggtttcaat gtgatgataa cttttgataa tcgttcaact
     2341 aaggtaatta cagcactttt atgtttcaga cctacgatag tgtcaccttc aaggtgacca
     2401 aactcatttg agaattggct ataatccttt tcacgttcat gaatagagcg gcggaaagtt
     2461 tgttttccac gtctttcttg atgtccattc ggtttacgct tgcctttcat cggtaagtca
     2521 gaagaatcaa atagcccctt tttgaacata cgataaatgg tacgagcaga acaactaata
     2581 ggaaacgctg cacgaccaac aatcacatcg ggtgtccatc cttgaacaac ctttctttga
     2641 atatattctg attgttcctc gggtaaaaca agcggacgtc taccacagtt tgatttgttt
     2701 ttcttatact gttgataata ttctaaggct gattttcctt gcttgaagaa cagatatact
     2761 ttatggatcg tttgtcttga acgattcaag caatgggcag ttttagcaac agattgattc
     2821 attttgaaat aagactctat tattacaagt tcatccgttg taagatgggt ataggtcatt
     2881 tgtactcact ccttataatt ctttggtcgg aactattttg agtgtagcac aaatgacttt
     2941 tttagttgtc tagcttaatt ttacaatcgg cgaataaaaa aagcgttagc aactatgctt
     3001 aaaaagcata tcgactgaat aaactaagca ttggacaggc tgaaagacta catttaaaat
     3061 gaaatgcgaa gaaacaatag aaaataaagt tgccttagaa ccatagttga atcacctaac
     3121 agtcgtgaag ggagaataag agaaatggca gaaaaacaaa ccgcaggacg agatcaatta
     3181 ggagattttg caccaaaatt tgcagaattg aatgatgatg ttttatttgg acaagtatgg
     3241 tccagagaag aattatctgc tcatcagcgt agtatcatca ccatatctgc attaatttca
     3301 ggaggaaatt ttgaacaatt gccagctcat ctgaagattg gcaagcaaaa tggcattact
     3361 agtgaagaaa tagcagaaat cattacccat ttagcttttt atgtaggatg gccaaaagct
     3421 tggtcagctt ttagtttagc aaaagaaatc tatttagaag aaaatagcga agataagaaa
     3481 gaggcgtaaa tgacgacaaa acacgaagaa atacacaatg gcgtgatttt tccagcaggt
     3541 gaaaaaaacg aagcttacgc tcaagtattt accgggcaaa gctatctgaa tatgttagtg
     3601 aatgatcccg aagtttctgt tggtgtaggt aatgtaactt ttgaaccagg ttgtcgcaat
     3661 cactggcatg tccataaaga cggctatcaa atcttacttg tgaccggagg agaaggctgg
     3721 tatcaggaag aaggaaagcc agcaagacga ttaatggctg gagatgtcgt cgtaacgcac
     3781 aaagggatta aacattggca tggtgcgaca aaagacagtt ggtttgaaca tttggcgatc
     3841 actgctggta attcagagtt tttagaacct gtaacagatg aagtgtatga aggattggaa
     3901 aatcaataat gtcaagcttg aacagctcct gaaagtagat tttaatcgaa ctttcaggag
     3961 ctgctttttt gattcagtct tttataaata gaaaataggg ttaataatag gaatgagaag
     4021 cacgtcgaaa gatcttaata ttgataatcg gctgggcata catgatttcc acgtcatcaa
     4081 tcaaactacc taacagatga taatcttccc gatcatgtcg gggattacct aatagttctt
     4141 gataatagat ttccatacta gggtcccgag gattgttcag caaatcagca agtacatcta
     4201 aatcttctgg ctgagtttct gccgtaccct gtatctctat gaaaagccct tcctcagcaa
     4261 attctactcc aatacgtatt tcagttattt catgggaata taaataagta agaagctcat
     4321 cgatgattct ttttgataat tctttttcca agtgtctttt tttcctttct gacagcggta
     4381 aagttatcta gtatcggtac aaggatacct gcaacgaaac cagttgaaaa accactgtta
     4441 tatagattta gtcctccgtg caaagtgctg acgttatgga caagagcaat atgtagtaca
     4501 ccagctatta caccgtaaaa actgccgtaa aaaccgctta tgggcgcgag acctgtccca
     4561 aacacagctg caacaagcat gccagtactg gtgatctcat gaaaagttcc aaacaaagaa
     4621 gcaataaata ttccgactag tatcggaaaa ctgtttttta aatgacaacc aaaagcactg
     4681 aaacccaccg cagataaaat agcaccgatg acaggtccat ttaactgacc gccaaccagc
     4741 aaaacataag aagacagcat cagaccaact aagcctatgt tgatcaaagt agcaccaata
     4801 ccagaattag caatgaaatc agttgtcaat tttccagatg aatcgaaaat ttctcgaatc
     4861 cccaaaagcc gaaaggaatt ataatagaaa ccaattccga ctgtcagcga aaaaaagcca
     4921 aaaataatcc aaatgagagg aaagtgatat tcattgaaaa ccaatgtatt ttcgacgatt
     4981 gaatagctga ataacctcaa aacagcagta aaagtcatcg caatcaaccc agaggtgaat
     5041 ccaatattat aaagtgtgaa tccacgatgg aaaactaaag tttgagcagc taagggcgga
     5101 agaagtagac caattgctat gccagctaaa ttccccaata aaatacctat cagtaaagga
     5161 aaacgcatac caaaagtgat ataactaacg accggtgata atgcgctacc aaacagtccg
     5221 atcatgatgt actgagaaaa aggtttattg acaaatacag cgtatagata cacacctaaa
     5281 atgattgaga tagaattata aaggtttttg ccaaaaaaag agaaaccggt aactgttaga
     5341 atcgaagcaa ataagggacc gtttaaaatc accttgtgcc gataagctaa aagcacagaa
     5401 agtaaggtca aaatccctac atttatgaat gcgcttccga aaccgcctaa agcaaaataa
     5461 tctgtcaaaa gattgcttgg cgaggtaaga atagttagca atccactcca attatcagta
     5521 ggagtattag aaagcaagcc aataaaaaga aaaaaaacac ctgtaaaacc aagataaaaa
     5581 tactttcttt tctgtgaaac gattcgtttt tgaggatgaa tataaataac gtcagtttcg
     5641 ttttccatta cacaccttct ttttcatttt ctttaactat aacttgaaat gaaagatttg
     5701 gacaactatc atttaggtca aaactaaaaa tgtaagggaa cccattgaat ttaaccaaat
     5761 ttttttaatt ttgcaaaaaa aagttaaaga agactgaaaa aaaatttaaa atataacgtt
     5821 ttgagtaaaa tgtttgcaaa aaatgctaaa aatagggtac aataaaacaa catatactgc
     5881 agtaaagaag tgggggagtt ttaatgagaa gaaaagtcta taccaaggat caaattctaa
     5941 aagctgctta tgatgttgtt gcaaaggaag ggtttaaagg atttactgcg agaaatattg
     6001 ctaaaaagat ggggatttcg acacagccga tttatctgga attcaaaaac atggaagatt
     6061 taaaaaatac gttgctggat tctatctttg aacaactaaa agaagatgtt ttccctattg
     6121 aacgtactgg tgataaaatc attgatctcg gtttgaacta tatctacttt gcaaaaaagc
     6181 atcatagtct ttacatggca ttgtacattg atgaacatgg ggacggaaag aaaatgcatg
     6241 atttttcata caattatttc cgtgaaatag ttaaagaaga tgaaagatac aatcaattat
     6301 cggatgaata tgtcgatgct ctgcatactg gtacatgggt agcagtgact ggcttagcgt
     6361 cattgatgtc atcaaacatc atgcatccaa cagaagaaca aatcattgca ctgatcaaac
     6421 ggacaattga ctcgatttta gcattagata atcctgaaat aatcagatag tgttctatca
     6481 gtatgaattt gatcggtaag tccgttggct gcaaactgtc aatggacttt tttgctaaaa
     6541 aaagcgattt ctgttattta tctattagaa ataggcttga catttcgtga aaaaagttta
     6601 aaaatagtac ttacggaagg ttggcagagt ggtaatgcac cggactcgaa atccggcgaa
     6661 ccggctaata ccggcgcgca ggttcaaatc ctgtaccttc cttatatcaa cgattagagg
     6721 ttgtgaagaa gctgtcctgc atcaagtaat aagaacggcc aaacaaaaat agcttttcct
     6781 attttttcgt ttgattgggc ttattaccgc agatgcaagc ttttgaacac cgtttgttcg
     6841 gaataagggg ctgtgacaag acttttgtca cagcccctta ttgttgtact tttaacctac
     6901 aatatttttt gattccccct gagtactatt ttttataaat gctagtaaat caatcttctt
     6961 tggccttttt ttgataaaaa aaatactaga tgtaagataa aagtgtaaaa acgatttgtt
     7021 ttaggacggg aaacgatgtg taccgcaatc acttatgcaa cgaaagacca ttattttgga
     7081 aggaattttg attatgagat gtcttataat gaagtggtca ctattactcc aagaaattat
     7141 cgtttcgatt ttagaaaggt aaaaaattta gataaacatt atgcaatgat aggaatagca
     7201 gctggggtat ccaactatcc actctattat gaggctacaa atgaaaaagg gttgagtatg
     7261 gcaggactga attttcctgg caatgctgat tataaagaat tacaagaagg aaaagataac
     7321 gttgcaccat ttgagtttat tccatggata ctagggcaat gctcaaccat agacgaagct
     7381 aaagaattat tggctactat caatctagtc aatatcgatt ttagtgaaaa actacccttg
     7441 tcacctttac actggttatt agctgataaa gaaaaatcga tagtaattga aagtatgaaa
     7501 gatggacttc atttatatga taatcctgtc ggtgtgttga caaataatcc gccatttgat
     7561 tatcaattat ttaatctgaa caattatcgc tcgctatcaa atggaacacc agaaaatcat
     7621 ttttcaaacc agatcagctt gaatgtttat agtcgtggga tgggaggatt gggcttacct
     7681 ggagatctct cttctgtttc tcgatttgta aaagccacat ttactaaaat gaatgctgca
     7741 tcaggtgatt cagaatctga aagtatcagt caattttttc atatactagg ctcagtcgag
     7801 caacaaaaag gagtatgcga tactggagaa agaaaatacg aatatacgat ttattcttct
     7861 tgctgtaacg ttgataaagg gatttattat tatcgtacgt atgaagacag tcaaattact
     7921 gctgtaaata tgaataaaga agacttggat cgtcatgaat tggtcagtta tccagtcata
     7981 aaagagcaac agataaacta tgtcaattaa ttaatgaata gatccgtttg gtatcatatt
     8041 ttctgataca gtcataatta gacacgatct ttaggtcgtg tctttttttg ctggtttttt
     8101 tggaaatagc atccactaat atttttttaa cacgaagaga ataaggtagc ctcaaaaaag
     8161 aaaacatggt aaattagata ttgggacttt attttctgta aaaacagaaa ctttcatctt
     8221 ttgttttcac gtgacgaaat tgtaagagag aaaatattcg tccgctattt attctatgat
     8281 aagatgcata tgaatgaaat aaacagtaat tttatattag gatgtgaaag ttaaatgtgc
     8341 ggtattgtag gttttgttaa cgacaaggac aacaaaaaaa caattattaa tacaatgatg
     8401 gatcgaatcg ttcaccgtgg acctaatagt tcaggagaat atatcgataa acatgttgct
     8461 ttaggattca gaagattaag tattattgac ttagagggcg gtactcagcc tatttacaat
     8521 gaagatcgga caaagatcat tatttttaat ggcgaaatct ataattacca gcctttgaga
     8581 gaagaattga ttgcagctgg ccacgttttt caaacacatg cagatacaga agtccttttg
     8641 catggttacg aagaatgggg aacagaatta ttgcaaaaga tccgcggaat gtttgctttt
     8701 gctatttggg ataatgaaaa gaatgagtta ttcggtgcaa gagaccactt tgggatcaaa
     8761 ccatattatt atgcagaaat gaacggaaca tttatgtttg gttcagaaat caaaagtttt
     8821 ctgcctcatc ctgattttaa caaagaattg aacaaagaag cgttgaaacc ttatatgact
     8881 ttccaatatt ctccattaaa cggcgaaaca ttcttcaaag atgtctatcg tttgcctgaa
     8941 ggccattatt atacgtataa agatggaaaa atagacattc agcaatattg ggatgcagat
     9001 tttgaaacaa aagaaacgca ttcccgacaa gaatggatcg aaaaaatcga cgaaaccgtt
     9061 caagcttcta ttgaagcaca tacggtcagt gatgtagaag ttggttcttt cttatccagc
     9121 ggggttgatt ccagttatgt aacctctgta ttgaaaccag atcattcttt ctcaatcggg
     9181 tttgatgata aaacctataa tgaagcgatt gaagccagaa aactaaccga actattggac
     9241 ttagataata cagcagcagt gatcgacgga gacatgtcgt tcaaagcatt ccctttgatc
     9301 cagtatcact tagatgaacc tgattcaaat ccttcttgcg tacctttgta ctttttagca
     9361 aatcttgctt cacaaagtgt gcgggtagtc caatctggag aaggggcgga tgaactgttt
     9421 gcgggatacc aaacgtatgg attccatacg aattcaaaat tcatccgtgt gattgctcaa
     9481 ggattgaaaa aattaccaaa aggaacacgc tataatttag gacgtaaaat cggaaaaatg
     9541 aaaaatttcc atggacggat ccatttatat gaatcacttg ctcctgcaaa agaatatttt
     9601 atcggtcatg ctcgagtgtt cgaagaatca gaagcatcag aagtgttaac accaaaatat
     9661 caaacagcac catctgtgga tgaaatcatg actgttcatt acgagaaaac agaaggaatc
     9721 aaagatgaag tcaataagat gcaatatgtc gatcttcatc aatggatgcc aaaagatatc
     9781 ttgttaaagg cagataaact ttctatggca agttctttgg aagtccgagt acctttattg
     9841 gatatcgaag tcatgaaatt ggctcaacaa ataccaagta agtttttatt gaatcaaaac
     9901 aacacaaaag atattttcag acaagctgcg aacaaacatc ttccggaaga atggtcgaat
     9961 cgtgtgaaat taggattccc tgtgccaatc aaagcttggt tgaaagaaga acacggctac
    10021 gaacaagtca aagcattatt cgaagcagat tttgcaaaag aattttttga tcaagaaaaa
    10081 atcatgaagt tgctagatga ccatcacgaa ggacgtaaag aagagcaaag aaaaatctgg
    10141 acaatcttca gtttcttgac atggtataaa gtctactttg tagatgaaac gattccgcaa
    10201 gcagaagcaa tcgactatgt gactgtttga tgaaaatcaa atatcaataa aaaagaggtt
    10261 ttctcctacc gtttaacact gagtgataag aaacaatttc aaaaaacagc tcttcccgtt
    10321 ttatagatat tatgacttat ggttcggagt tggcagagga acaccattgt ttcgaacata
    10381 agggggttgt gacacgattt tcgtcacaac ccctttacat atgttcaata atcgatttct
    10441 ttttcttttg tcccatcttt tttataaatg aacaaagttg attctttgtt ttgtgctact
    10501 tgctttcctt tttcgattgc ttcttttttt gtatcaaaat ggttgcttgc cttttttgcc
    10561 cctttagatt tcacgatcca ttgttcttcc tcatattcta cgatcacatc tgaatcaagc
    10621 agttttccag cacgaggatt cgtatcgtgt tcatccgtct tgcttggatt tttttctttt
    10681 tcgaaagttc gtttttctga ttcactagca tcttcatacc atttttttgc ttgatcaatg
    10741 gcaattggga tcgcacggtt atcagggtac ccttcatcta ataatgcatt tgcgatgtcg
    10801 attgcttttt ttcttgtcaa tttatctaag tttttcattg atgcaggaaa gtccttcatg
    10861 ttccatggca tattagtatc cccctttatt aaaaaacata atctatataa acaagtgtaa
    10921 gggtatttat acaaaaagaa aaagattttg tttgagaagt aaaaccgttg tatttatcgt
    10981 caagtagttt tacttaacaa aatcgtgaaa aacactagca atctagaaaa aatcatggta
    11041 agataatcaa gtctgagagt catcttagag gattcttgta aaaatgggag ggaaaaacat
    11101 gcgcgtaaac atcactttag agtgtacttc ttgtaaagaa cgtaactatc taacaagcaa
    11161 aaacaaacgt aacaatcctg atcgtttgga aaaacaaaaa tattgcccac gcgaacgcaa
    11221 agttactttg caccgtgaaa caaaataatg tttttaaagt tctcaattcc ttgctggtca
    11281 aagaattgag acttttttta tctcaaaaca tctaaaatca taaggagcgg ataatatgtt
    11341 tatttccatg tgggcacaag ataaaaatgg tctgatcggc aaaaatggtt tgttaccatg
    11401 gcgattgcca aatgatatgc gtttctttcg cgaacacaca atggatcgaa ttttagtgat
    11461 gggaagaaag acctatgaag gaatggggga tctttcactt ccttatcgcc atatcatcgt
    11521 gttgacaacg caaccaaatt ttcgcacgaa agaaaatgcg gaagtaatgc attcgatcga
    11581 tgaacttttg tcgtatagtc aaacgatctc agaagatatc tatgtttcgg gtggtagtcg
    11641 gatttttcaa gaacttcttc cttacactgg tcggatctgg cgaacattga tcgatagcac
    11701 gtttgaaggc gatacgtata ttgggaatat tgatttttca gacttcgccc ttatagagga
    11761 gtatgagggg ataacggatc aagaaaatct atatgcccat cgttttcaaa aatgggaaag
    11821 agtaaaaaat agggttgctt caaaagaaga ataaaatcta tgcgtctagt ttgttaataa
    11881 gaaaaaaagc atctcacgac taatcgtgaa gatgcttttt ttagttaaaa tcaatttagt
    11941 ttttctttga tatcattgac catttcctga ctggcaatag gtccgttata tagccaacta
    12001 gattcaggtc cgaattgcag tacatgattg tctgtcactg cttttagatt cttccagctt
    12061 ggttcattga acatggcagc atctttgtca ctgttcacaa ggatgatgta gtcagcatct
    12121 aattcagcta gcttttcaga ggaaacagca gaccagtcag aagtggcttt tttggagacc
    12181 tcttccacta gatcaggaac accaaattcc agatcgtcgt atacgattct tccacttgac
    12241 cgatttttgc tgaccataaa ggctgagttg ttggtcaccc acaacactgc agctgtcttg
    12301 ttctcgattt tatctgctag ttcttctcga gttttcttga tggatttttg ataatcggaa
    12361 atgatttttt ctgcctcttt ttcttttgcc aaagctttgc cgacatcttt tagttgttct
    12421 tcccatgtga catctgttcc gttttttaca acataagttg gcgcgatttt gctatattgt
    12481 tcgtatttcc cgccttcgac agtagcggaa gaagaaatca acagcaaatc aggctcaaag
    12541 cttaacactt tctcataagg aaggtcataa gaaatcgttg ggacatcctt taaatcatct
    12601 tgcagataat cttgaatgga accactgcca acagtccact gggcgatagg tttttcccct
    12661 agtgcaatca gatagtcttc taagtaactg ccgatgatcc gtttgggcgc gtttggaatc
    12721 tctactttat gtccaagtgt atctgtgact gtatgagtac tattggatga agtcgtcgaa
    12781 ttggcagttg tagctgtttc tttatctgaa gagggggagc aagcgctcag tactccagcc
    12841 agcagcaata tgctagataa tgtgaatact tttgttttta atttcatttc tttcctccta
    12901 acataattga gaatctttct cattggttat tattcctgat atttcgaacg ctgtcaaccg
    12961 cttcatttgg ctatagatat gaaagtagcg cttgacgaaa tgttgaaaca gcgtcataat
    13021 tgagaatgat tttcagcgaa aggatgggtg aaatgacgag attaagagca gctgacattg
    13081 cagccggcta cgaaaacaaa aaaatcattg aaaacatgtc tgttacgatt cctgaaggga
    13141 aaatcactag tttgattggg ccaaatggaa gtggaaaatc tactttattg aaaagtttta
    13201 cccgtatact acctgtgaaa tcaggaaaga tcattgtcaa taactctgat atctctacat
    13261 acagttcaaa agaacttgca aaaaaaattg cattattagc acaaactagt gaacacccta
    13321 caggtatgac agtagaagaa gtagtatctt acggacgata cccttatcaa aaactgttca
    13381 gtggcatgga cacgcaagat catgaagcaa ttgagtgggc gttggatgct acaggtctag
    13441 ctggatttcg tatgcgggat atcgcaacgc tgtctggtgg acaaacccag cgtgtctgga
    13501 tcgctatggc gcttgctcag gaaacagata tcttgatctt ggatgaaccg acaacatttt
    13561 tagatccggc acaccaatta gagatcctac agttattgac tgaaatcaat caaatgaaac
    13621 aaacaacgat cttgatgtcg attcatgaca ttaatcatgc ttcacgtttt tcagattatt
    13681 tattagcaat gaaaaacggt aagttgatca cagaaggtac acctgaagaa gtgatcacaa
    13741 aagagtgtat gaaaaaaata tttgagattg atgcaacgat tgtcacgctt ccaagaacaa
    13801 ataaaccagt ttttctaacc tatgatttgt tgccaaaaga gcaggaggat caattatgag
    13861 gactatgaag aaaaatagac tcctgctttt ggtctgtttt gttcttttga ttattttttt
    13921 tgttgctctt tcaaatggcg cagcgactgt gggaaaagca gatttttggc aggcgttgtt
    13981 tgattttgat agatcaaaac aaagtcacca aatcatacga aatattcgtt tgcctcgtgt
    14041 ggcgggagca tttcttgtag ggagttgttt tgcattgagc ggtgcattga tgcaaggagt
    14101 gacaagaaat cctttagcag attcaggctt actaggaatc aacgcgggtg cttcgttggc
    14161 aatggccctt agttttgctt tttggcctaa agcttctgat ttttctgtgt ttctaatgtc
    14221 attggcaggt tcttttgctg taactttgct cgtatttggt tttttgcagt tttcgaaaat
    14281 gggcatgaat cctttacagc tgattttagc tggcgtagct attagttctt tttttactgc
    14341 tatcagccaa gcacttaccc aattctttaa tctgcagcag gatcttgctt tttggtttgt
    14401 aggcggagct gccaatatca catggaaaca attagcagta ttgtttcctt tatatatact
    14461 tgctgttttg gtttcttttt cattaggaaa agcaatatct ttgattcatt tgagtgaaac
    14521 acatgcatta gctcttggaa gtcatcctaa acgaacgcgg ttgattgttt tctgtctagt
    14581 tgcagtgtta gcagccattg ccgtttcgtt agtcggacct gtttcattta ttgggctgtt
    14641 ggttccgcca gttgttcgcg gaatggttgg aacggactat cgttatgttc tacctgtctc
    14701 ttttttagtt ggcggtggat tggttatgtt ggcggatttt acagcgagga tgatcaatcc
    14761 cccttttgaa acaccttttg gattagtcat tgcattgatt ggcgttccgt ttttactgat
    14821 acaagtaagg agggaacaac ggtgaaaaaa atatattgga cgttctattt cttgcttttt
    14881 gtacttgtca tcctgtctat aggtagttta atgattggta cgccttttat ttacctggat
    14941 cagctttttg ccacgttcat agggaaagaa tcatcttctt tggcgctgat tgttagagaa
    15001 tttcgagttc ctcgattact gatcagttta ttagcaggag cttgtcttgg cgtttctggt
    15061 tatatcttgc aaggagtaac aagaaatgaa ttggcagatt ctagtatatt gggcattaat
    15121 gccggagcag gatttttagt gatgctttat ttaggtttct tttcgcacta ttcgctgccg
    15181 tttctattgc tagttgtagc ctttctaggt ggtgttttag ctgcttgctg tgtctatctt
    15241 gcggcctatg atcgaaatgg ctttttgggt atgaatcgaa tcttattgtc cggtattgcc
    15301 gttaatgccg gcctttctgc attaacattg ctatgcacga tccaattatc taaggaaaat
    15361 tatggatttg tgaatgcatg gttagcggga tcaatctggg gtgcgagctg gccttatgtt
    15421 tgggcattgc ttccatgggc ggttatctta attccgctag gaggacttta tgcacagagg
    15481 attgtattgc tatcatttgg tcaagaacgc gcgcaaggat taggccttaa cgtggctaaa
    15541 agtcaaaaaa tccttttgtt gttagctgtt gctttggcaa gcggtagtgt agcggtgaca
    15601 ggtagtttat cttttgtagg actactagct cctcatgctg cgaagctagt cgtacgtaaa
    15661 gaaaataact ggacctttgt tctgagcggt ttgttcggtt ccatttttct gcttagtgca
    15721 gatatggttg ctagagtgat tctactaagt ggggaagtac ctacaggaat tttgattgcc
    15781 ctttttggtg cgccatattt tctgtatgca ctttttatca gacaaaaaca cgcgctgaac
    15841 agttgaaatt cattaaaaaa ggaaggccaa tcatttctag gtcttctttt tattctattt
    15901 atgcatttat ttgaaagttc gattgctatt tttctttttt tcctatacca ttttatgaga
    15961 gaaggaaggg tgagtttttg gaccaattga aagtaagatt acgccggaaa ggaatcgaaa
    16021 agctgaaaaa tctggccaaa agtggcaaga aaaaggaaaa agaacagcga atctataaac
    16081 agctattcgc cagcgagtac tggaagtcag caaaagtgat agggacgact ttatcgaatg
    16141 aatttgaact ggatacacag ccggttatcc aacgagcaga aaaagaacaa aagaaaatag
    16201 ttgtacctaa aactttgccg aaacgtcaga tggcgtttta tgaaatggat gaacagtccg
    16261 ttcttgtacg ttcgagtttt ggtgttcttg agccacaatc agagaagctc tatttgccgg
    16321 agatgatcga tctcctcatt gtaccaggta ttatctacaa tagagccggt tatcgaatcg
    16381 gttttggcgg tggttattac gaccggtatt taaataattt tcataaaaaa acatgtagcc
    16441 ttgtttttcg tgaacaattg aatgaacaat ggcaaccgga gagctttgat cagaagatcc
    16501 aacaattgtt tattgactaa aatgaaatgg gaggttttca tgaattatca acagcaaata
    16561 aaattgcgtg catggttaag acgaccgtta tggacctatg cttttttagg gatacagaca
    16621 gcggtcttcc tcatgatgga attatttccg cgacttgaga tcccttatta tgccggaatg
    16681 tatggtcctt atcttgttca cttcaatgaa tggtggcgtt tagtgacacc aatcttcatc
    16741 cattttggac tgatgcattt cgtaatgaac tcactgatac tgtattttat ggggcagcaa
    16801 atcgaggcaa tctatggcca ttggcgtttt ttcttgattt atatgttcag cgggataatg
    16861 ggaaatacag caagctttgc ttttaacgaa gcgaatgttc tgtctggtgg tgcaagtact
    16921 tcgatattcg gtttgttcgg tgcgttgttt atcttagggt ttcattttaa acataaccca
    16981 gcgatccagc agctagtcag acattttctt ttatttattg tgttgacatt tgtttttggt
    17041 cttctggata cctctgtaga tgtttgggga catgtcggtg ggctaattgg cggattagtg
    17101 ttagggaatg ttttgggtct accgaaacaa cagacaagct attcgataca ccaaaggatt
    17161 ctttctacac tagtttttgt atttcttttt gtaatatgta ttttactggg tttaaaaaaa
    17221 tatggtttac ttgtataata tgaaggaagt gcttaaagat ggagagctta tatgatgtac
    17281 agcagctgtt caaaaaattt ggcatcttta tctatgtggg tgcacgcatc tatgacattg
    17341 aattaatgac tatcgaattg cgtaatctgt atgatagccg tttgattgat catgatacat
    17401 acatgacagc gtggcgcata ttaaaaagag agcaccgaat tgaagaaagc cgaaagaaag
    17461 gaaatttgta atggacaaga agattattgg gattgattta ggtggaacaa cagcaaaatt
    17521 tgcaatttta acaccagaag gagagattca gcaaaaatgg agcatcgata ccaatatttt
    17581 ggatgatggt aagcatatcg ttcctgaaat cattgaatca atcaatcatc gtttgaattt
    17641 atataatatg aaagccgaag actttatcgg aatcggcatg ggtaccccag gaagcgtgga
    17701 cagcgaagct ggaacggtta ttggtgcgta taacttaaac tggacagaag ttcaatttgt
    17761 gaaaaaactg atcgaggcag gaacaggaat caaattcgct atcgataatg acgcgaatgt
    17821 tgctgcttta ggtgaacgat ggaaaggtgc tggagaaaac gatccagacg ttgtattcgt
    17881 tactctagga acaggtgttg gcggaggtat cgttgctggc ggaaatttga ttcacggcgt
    17941 gagcggagct ggaggagaaa tcggacatat cactgtggat cctgaagggt tcgaatgtac
    18001 ttgcggtaaa cgcggatgtt tagaaactgt atctagcgct acaggtgtgg tacgcctagg
    18061 tcgtttcttg gcagaagaat acgcagggga ttcaaaactg aaagcaatgc tagacaacgg
    18121 agaagaagta acaagtaaag atatctttga aatggctcaa gaagacgatc cgtttgcatt
    18181 gatggttgtt gatcgcgtat gcttctactt aggtttagct tgcggaaact tggggaatac
    18241 attaaatccg tcaagtatcg tgttaggtgg aggagtatcg gcagcaggag aatttttacg
    18301 tagccgtgta gagaaatatt ttaaagaatt tactttcccg caagtaagag aatcaacaaa
    18361 aatcaaatta gcagagcttg gtaatgaagc tggtgtcatt ggtgctgcct cattagcctt
    18421 gaaatttgct taaaaaaacg gaggcattac atggttttat gggtaattaa tatcattttg
    18481 ctattgattg tgcttgcaat catcctttgg gaagtctatc tacgagtaat ggccaaacgt
    18541 tcagcaacga ctttgacaga agaagaattt cgagaaggaa tgagaaaagc acaagtaatc
    18601 gatgttcgcg aaaaagatgt gtttgatgct gggcatattt taggtgcaag aaacatccct
    18661 tacactgttt tgaaagattc attaggatct attcgaaaag accagcctgt ctatatttac
    18721 gatcaaaaga aaagtttgag catccgtaca gcaaataaat tacgtaaagc aggttatcaa
    18781 gagatttatt tacttaaggg tggatatgac ggatggtctg gaaaaatcaa aagtaaaaaa
    18841 gtataaaaca gatgattgta tgacagcttt aaaaaccgaa tcaatcatta gattttgacc
    18901 aagatcacat gaacggcgca atggatagtt gtgccgttca ttttttttat tttgtgctat
    18961 tatctacata tataagctaa gcgaaaaggt agaaagaagg aaacaactat gatcaaatta
    19021 aaacgtgttt accttgatgc tgaaaaagag gatggctata gaatactcgt ggatagattc
    19081 tggccacgag gaatttcaaa agaaaaagcg cagttagatc tgtggttgaa agaaattgca
    19141 cctagtaatg aagcacgcaa aacttttgac catgtaccag aacgatttgc ttcatttgca
    19201 aagaaatacc agcaagaatt gattttcgaa gaagaaaaga agcaagctat ttttaaactc
    19261 agtcaactta tgaaagaaca tccagtcatt acttttgttt atggtgcaaa aaatgaacat
    19321 cagaaccatg ctgttttatt gaaaaaattt atcgaaatgg aggcatgcaa atgaaaatag
    19381 ggatcattgg tgccggacct agaggattaa gtgtagcaga aaggattctc cataataatc
    19441 aggacagaca ttctcttgaa ctttttcttt tcgatcccaa tggtcctgga ggtcgagttt
    19501 ggcggttgaa tcagccaccg gagttattaa tgaattctgt ttctcaacaa gtgacgcttt
    19561 ttacagatga aacattgtcc agtggaggat tggtaacaca gggacccaac ttgtttcaat
    19621 ggagtaaagg acctgctaca gattatatcg aacagacggt aaccaaaaat aagcaactaa
    19681 ttatagaaga aatcaatcgt ctggaaaaag atcagcaatc tactcgatgt ttatacggac
    19741 tctatcaacg ctggtttttc tcaaagttgc aagaagaatt tccagcttct attcagtttg
    19801 tttattcttt agtaaaaaaa gtaagcaaaa gaaaagatgg ttatctttta cagacagatc
    19861 agcaaagcta ttctatcgat cagcttgttt tagcaagcgg tcactgggaa aatgatgaga
    19921 cagaagaaga aagagtgtgg gtagactacg caaagaaaaa tgttttactg tatcagcttc
    19981 cttctaatcc tgcagatgta gatctttcga agataccgcc taaagaaaat gtctttttaa
    20041 gaggtctagg cttaagtttt tttgattatg tcggattgtt tacagtatca agaggagggc
    20101 gcttcgagaa cgaaaaggga aaactggtct atcatccatc aggcaaggaa ccgattgtat
    20161 acagtggatc aagaaggggg ctcccttatt accctcgagg aagaaatcaa aagcaaggcg
    20221 gagcaatggc atggccacgg ttactaacaa aagaaaacct ggagcaatgg catcagtcgg
    20281 gatttttgac tggcgaaaca ttttttgact atctgaaaaa agacgttgag ttgttttatt
    20341 acaagaaaat catcgaagaa tatcacttgc cgattccaaa gaaaaatttt gagcatgatt
    20401 ttctctccct ctcttcagag gagtgtctcg gaaagcatcc agcactaatc ccttatagat
    20461 gggattggtc gtatttagaa actcctcttg cctatcagaa tgagcccttt tcagaagcta
    20521 gccgaatatt tattgaaggg cagataaaag aagcggaaaa aggcaattgc acaggagcat
    20581 ttgcttcaac atttgatgca ttaaaagact ggcgtgatcc aatccggcag gctattgaat
    20641 ggcacgtttt ttctgctaag gaatacaaag aaatactatg gggatggttt actcatctaa
    20701 atagcttttt aacgatcggt ccgccaatca ttcggacgaa agaattagca gcactgatag
    20761 atgcgggtat ttttcatttg atcgaaccgc catttgatat tcagcaaaag cacaaccgct
    20821 atgttatttc tctacaagat aaagaaatca gcagtcgcta tttgattgaa gctcgattgc
    20881 cacaaacaag cttttctcgt tcaagtaatc ctgtcataaa aaatttgaag gaaaacaaac
    20941 ttgttcgccc tttttcattt tcagataata caggaagtta tcattcagaa gctatagatg
    21001 ttgagcgagg tacgaatcgc gtgattgatg cttcagggga aatccagtct gacttttatt
    21061 gctatggaat acctgttgaa ggtgtggatt ggctaactgc agcagtagct agaccttata
    21121 cagatccatg gaatctgaga caggcagata aaatagcaca acttatattg actgacaaat
    21181 aaaaatacgc atgatgactt atctgttttt tagaaagtct tcatgcgtat ttttaaataa
    21241 ttattaacga gaaacgcgtt tacgcattgc ttttttacgg ttttcttcga tcattgcttc
    21301 tttttcgtca attggatcga tcactgtttt tttcacagaa gcaaccaaac cagcaacggc
    21361 agcggctgtt gccagacttc ctactaatat tccagataca aattttttca ttgaatccac
    21421 ctcatttatt atttgttata tctattatgg aacaaaagag aaaaaaaggc tagttttacg
    21481 tttgtttgta aac
//
LOCUS       DACECZ010000035        21489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.035, whole genome shotgun sequence.
ACCESSION   DACECZ010000035 DACECZ010000000
VERSION     DACECZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.035"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            51..464
                     /locus_tag="IWO35_001259"
     CDS             51..464
                     /locus_tag="IWO35_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0316324.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            1282..1626
                     /locus_tag="IWO35_001260"
     CDS             1282..1626
                     /locus_tag="IWO35_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0316325.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            1631..1912
                     /locus_tag="IWO35_001261"
     CDS             1631..1912
                     /locus_tag="IWO35_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316326.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVRESLNKDNNVERIKNIFGYGIEEINYYIDFAEAFIPMVR"
     gene            2016..2330
                     /locus_tag="IWO35_001262"
     CDS             2016..2330
                     /locus_tag="IWO35_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ0316327.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            2308..4002
                     /locus_tag="IWO35_001263"
     CDS             2308..4002
                     /locus_tag="IWO35_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ0316328.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            4022..5200
                     /locus_tag="IWO35_001264"
     CDS             4022..5200
                     /locus_tag="IWO35_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0316329.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            5163..5849
                     /locus_tag="IWO35_001265"
     CDS             5163..5849
                     /locus_tag="IWO35_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ0316330.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            5849..7009
                     /locus_tag="IWO35_001266"
     CDS             5849..7009
                     /locus_tag="IWO35_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0316331.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            7019..7894
                     /locus_tag="IWO35_001267"
     CDS             7019..7894
                     /locus_tag="IWO35_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316332.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFEGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            7891..8202
                     /locus_tag="IWO35_001268"
     CDS             7891..8202
                     /locus_tag="IWO35_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316333.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            8192..8545
                     /locus_tag="IWO35_001269"
     CDS             8192..8545
                     /locus_tag="IWO35_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ0316334.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            8535..8936
                     /locus_tag="IWO35_001270"
     CDS             8535..8936
                     /locus_tag="IWO35_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316335.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            8929..9333
                     /locus_tag="IWO35_001271"
     CDS             8929..9333
                     /locus_tag="IWO35_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316336.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            9345..9926
                     /locus_tag="IWO35_001272"
     CDS             9345..9926
                     /locus_tag="IWO35_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0316337.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKLQL"
     gene            9972..10334
                     /locus_tag="IWO35_001273"
     CDS             9972..10334
                     /locus_tag="IWO35_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316338.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            10337..10519
                     /locus_tag="IWO35_001274"
     CDS             10337..10519
                     /locus_tag="IWO35_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316339.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            10536..13967
                     /locus_tag="IWO35_001275"
     CDS             10536..13967
                     /locus_tag="IWO35_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ0316340.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGDTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            14018..14755
                     /locus_tag="IWO35_001276"
     CDS             14018..14755
                     /locus_tag="IWO35_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0316341.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            14765..17056
                     /locus_tag="IWO35_001277"
     CDS             14765..17056
                     /locus_tag="IWO35_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316342.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDVDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            17080..19155
                     /locus_tag="IWO35_001278"
     CDS             17080..19155
                     /locus_tag="IWO35_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316343.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSSGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDDQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGNPNLMANINADSFS
                     QGSGALSVVDDGDEVVVTLDPNNKLEIFKAKSQPALVVGKTYTMSVEIMLEGDFTGDP
                     SKIGLRYIKMPNWVSELYTRNTLTATKGVWQKLTGTVKITAASDNAESWLIMLQNKDA
                     NNSLSGKLRLRHAKVEYGSEATPFQPNLLVEPYNICREYPNENLCNPTVKFPIKSSVE
                     TLYSGRVPEDFVKGETYTVTIGATKPSTQSFGVYLALENHAIFKSVEGLPNTWTATLK
                     IDRLGEKKNAVYIYQSPASSVGACQIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNNPK
                     DYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQ
                     TKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGK
                     RSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSD
                     YTFAITYTI"
     gene            19318..19764
                     /locus_tag="IWO35_001279"
     CDS             19318..19764
                     /locus_tag="IWO35_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316344.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19766..19903
                     /locus_tag="IWO35_001280"
     CDS             19766..19903
                     /locus_tag="IWO35_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ0316345.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            19941..20234
                     /locus_tag="IWO35_001281"
     CDS             19941..20234
                     /locus_tag="IWO35_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316346.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            20231..20455
                     /locus_tag="IWO35_001282"
     CDS             20231..20455
                     /locus_tag="IWO35_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0316347.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            20452..21477
                     /locus_tag="IWO35_001283"
     CDS             20452..21477
                     /locus_tag="IWO35_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0316348.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNPYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNYAHIDQFGEIENANLHVAGWHIANYQYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPQR"
BASE COUNT         7560 a         3266 c         4567 g         6096 t
ORIGIN      
        1 taataaatcc gacaagttta ttatatcaca taaaggagcg gtttgacttg atgcaattgt
       61 tacgagaggt agatttcaaa cagacaagat gtaatgcgag agatgtgctg aagaactttc
      121 ggcgtttgga gcggatggca ggtcgctctt tgatagatat taagtcgccg attattacgg
      181 atatgccgaa ggcaccgaag cacggcaata aggcagagga cgcgatcatt cagatgatgg
      241 atatagaagc ggagagagac gcgattttag cggctttgat ggctcttagt ctgattagtc
      301 gtcagatact ctactacagc ttttgtgtgc cagacagctt ctcaaactac agaattagcc
      361 gtgaagtggg ttattcagaa agaagtatac aacggatgaa atcggaagct ctaatagagt
      421 ttgcagaagc atataaacac ggaagaataa ttgcttataa ataatttggc ggttttttgg
      481 cggaatgatg gcggttttta gctatttacc agtgatatta tgatagtgtc gaaagatttg
      541 gacaggatcg acaaacaaga tagacagcag cggaaatatt aagagtaagg atgtgaattt
      601 caactccttc taaattgttc ttattatcta tcatccgttg ctgtctattg tttttttaat
      661 tattcacacg ataactaaag gtgggtgaag agaaatgatg gcgttaataa tttcaatttt
      721 tgcgctctgt ctgaatgtct atatgattgg atttaaaaat gggcaaaata aaagatagta
      781 gtaactaaga aagaaatttt tatattgtca ctgtggcgga aagggtagac gctaagcatg
      841 tgtgctaggt caatgcttcg gcaactatgc aatgttcgat tcattgccag tgacttaagc
      901 agcttgcact tggtagacat aaatacctga tgtgtcgtag agttcaaatc tctgaatcta
      961 ggtgaggtcc gtggtagaac ctattagggc gggctgttgg ttcaattcca actttggtgc
     1021 aagttgctat tacatattag atcactcgtt gagtggtctt tttattttgc acaaaggagg
     1081 taacaacaat gtatagacca caatacttag aacagaagta tgaagtaatc actgtgcaaa
     1141 atggtaacgg tgagatagta cgaaagtata gaagaccaat aaagagcgat acatataaac
     1201 gaaaggaaag gaatgaagtt attccattgt atggcaaaag aatagctaag cattaaataa
     1261 gattgcgaaa ggagacgggg catgaccgag gaattctata gatggctatt acagttgata
     1321 agagaagatc gtttggttaa gttctatcag tctcctaaat ggcgcaggct tagagagaaa
     1381 gcgatgaaac gagatcacta tgaatgccaa gagtgtagaa gactaggtaa gtatcataga
     1441 gtagagaacg ttcatcatat aaaggaagtc aaggatagac ctgacttagc tttagattta
     1501 gataatctta tttgtttatg tgttgaacat cataatgaag ttcatggaag atatcttaca
     1561 gcgttagata aacaagagaa gaagatagaa agctttgcta acttcgacgc aagtgaaagg
     1621 tggtaagtgc atgatcatca atgacaatgg cagagagtat gatacagaaa agattgaaga
     1681 gtattcatct tatacacaag gattaattaa acgtttgata tacgttcgct atgtaggtat
     1741 tagggatctg ttatcagata actgttgtag taaatacaaa gtgaatcaag tgagagaatc
     1801 attgaataaa gataataacg ttgaaagaat aaaaaatatt tttggatatg gcattgaaga
     1861 gattaattat tacattgact tcgctgaagc tttcattccg atggtgagat aacccccccc
     1921 ttaaaataaa tcgcaaattt tttgggggtg atgaaacgga gggggctgtc aggaaaagag
     1981 attttttcga actttatcat gaaaggaggg ctaaaatgtt taaaaacgaa ttgtctcaaa
     2041 atcgctacag agaaaaatta cgccgctctt taataagcca attggaaagt cagaaaacaa
     2101 atattgagcc attcttagat aatgttgatc gttatatcag tttatgggaa acggcgatat
     2161 cactggaaga agatatatcc gagaacggca ttagactgga gaatggtaaa aagaatgaat
     2221 cagtagcgtt gcttgtttct gtcaacaaac aaatgggatt gatgttggat aaacttgcca
     2281 ttactcctga attggtaggt gaagcaaatg aatcaattcc tgagttataa gcatattgaa
     2341 aattggttca aagctataga agaaggcact atcaaggtat gcaaagagca attattgcta
     2401 aaaaattatc tagaagaaag agtctttact agagaagata tttacttcga taagcagatg
     2461 gtagaggatt caatcaatat accagcacaa tactttccat tcgaattaat tccgtgggaa
     2521 aaatttctac aatgttttat ttatggtgtt cgatggaaaa aagataaaac actagtgttc
     2581 aatagatatc tttcattaat gggacgtggt aatggtaaaa ctggttttgc ttcttggaac
     2641 aacttctttc tactaaccgc taaacacggt attaaaaatt atgatattga tatctatgcc
     2701 aataatgaaa gccaagcaaa gactagtttt gatgatgtat ttaaagtaat taaagatcat
     2761 cctgatttag ataaaaaagt atttaaagct acgaaggaag ttattcaaaa tatcgctaca
     2821 aacagcaaac ttcgttataa cacggcaaat gctagaacaa aagatgggaa gcgaccaggt
     2881 gcaaaccgct ttgatgaaat tcacgaaaat gaagattatt caatgataaa tgtggctact
     2941 tctggtggtg gtaaaattcg agattataga gaattttatg atacaactaa tggtcatgtt
     3001 cgtggtggtc cgcttgatga cattatagaa gaatcaaaaa tgattctttc tggagaactt
     3061 ggaattgaca aggatggagc agaattttct agtttgtttc catttatttg tcgcttggat
     3121 aacgataatg aagttgatga tcccgacatg tgggaaaaag cttgtccaac tattaattac
     3181 aatgcagatt taaaacggaa aatgtttcaa gaatactctc aaatgcaacg taatgctggt
     3241 ttaagactta cgttcatgac caaacgaatg aacagaccta tggaagatac acgatttgct
     3301 gttgcttcat atgatgatgt tctgcatacg aaagaaaaag aatttcctga aaaaatggat
     3361 gaagtgatag gaacagtcga ttttgctgat agacgagatt ttgccagcgt tgggttgcta
     3421 ggaaaatacg ataaagatgt ttattttaca caacatactt ttatccacga atcagccctt
     3481 cgattacaaa acatcaaacg agaggttata gatatttcta tagatcaagg aaaatcacag
     3541 atcgttcatg gaaaaaatat agaagctgat tatattgtag gttggtttct tgaaatgagt
     3601 aataaatatt atattaaaaa aatcgctatg gatatgtacc gtgcaaaaat attgaagccc
     3661 gctttagaag aagcaggttt tactgtggaa attgttcgaa gcggatctgt tacacatggt
     3721 atgttaaaag atctggttga tgaccttttt attaatcaac gtttattttt tggtgacgat
     3781 gcgattatgc gttggtattg catgaatgta tatgaagagc atatttctaa tggaaatata
     3841 cgctatgaaa aaatagaacc tgaaactaga aaaacggatg gctttttttc attccttcat
     3901 ggtttgaatt ttttagatga tatttatgat tctgctcctg taacagtcac aaatagctca
     3961 gtagaaaata caggaactgg atttactcct ctagtattct aacttgaaag gaggtgagaa
     4021 agtggggatt tttcaaaagg cggtaggata cttcacaaaa aaagcaacgg ttcctttaga
     4081 agaatacttt tgtaaattgc aagttgattt tgtgtatcga aaatttgcga ttgaaacttg
     4141 tattgatttg attgcaaatg cgatgagtaa agcggaattc aagtcatatg aagatggaaa
     4201 aaataaaaag aatgatcttt actataggct gaatgtagct cctaataaga aaaataatgc
     4261 aacagaattt agaaaaaaac tgatcaggag attaatattc tacaatgaag tattgatcgt
     4321 ttctccgtct aataattcta gcgaaatatt tattgcggat agttgggatg tcacagaata
     4381 tgcattgaaa gatgatgtgt tttctcaagt gcaaattaac aacatagtcc ttgatagaga
     4441 atttctagaa agtgatgtta tctatataaa atacgcagat caacaaatta ggcaactagt
     4501 cgatgcgtat tatcaagcgt atgggaaact catttctagt gccatgaatg tttacaagcg
     4561 ctctaacgct cgtagatacg tactgaaagg gaatttattc cgaccgcaag acaatacaac
     4621 acaagatcaa atcaataaaa tgatgacatc acaatttaag gcttttatgg aagctgataa
     4681 tgcaggtgcg gtatttcaat tacaaaatga gtacacatta gaagatttca gcggaaactt
     4741 ccaaagcaat tcaagagata taaaaaactt aatagacgac atctttgaga tgacagcagc
     4801 agcgtttcac gttccgaaaa acctactaaa gggagacatg agtgggttat cggatcaagt
     4861 ggacgctttt ttaatgttcg aaatcatacc gattgctgaa cttattcagg atgcgtttaa
     4921 cgctagtctc tatgaagtag aagaatactt gtcagggaat tttgtacgtg tggatacaac
     4981 tatgatcaag attactagct tcaaagattt ggttgacgct attgatgtag gcattagaaa
     5041 tggagtattt acaatcaacg aaggaagaga gcgcgttgga aatgatcgct ctgataaggc
     5101 gatggcagat gaaatattta taactaaaaa caatcaacaa gtatcgaaag gaggtgaggc
     5161 gaatgacgac aatgaaaaca tttctagcag taaagaatga aggcgcagta ccgcaaattt
     5221 ttattcaggg atttattggt tctagttggt tctttgaagg gaatactgac aagggaatca
     5281 aaaatatttt ggatagtcta ggtgatcaag aagaaattga agtagtaatt aattcaaacg
     5341 gtggagacgt atttcaaggg attgctattg ggaacttact taagtcaaat aaagcaaaag
     5401 ttaacgttgt gattaacggc ttagccgcta gtgctgcttc aattatcgca atggctggcg
     5461 atactataaa aatctacaac aatgcacaat tgatgattca ccgcgcttcc acatacggag
     5521 aaggaaatgt cgatgacttc cgtacgattg ctgaccaact ggaatcaatt gataaatcgg
     5581 taaaggcttc atataaaaca cgattcaatg gcacagatga agcattgcaa gaacttcttg
     5641 aaaaagaatc gtttatggat gcagaaacag ctttgagtta tggattggtc gatgaaatta
     5701 tcgatgcaga aaatagctca ggtactgaag ctaaaaaaga acaaagcgtt gaagaaattt
     5761 tgaatgacgt taaagaaaaa agagcagaaa aaattgctgc atttacagca gcattaaata
     5821 aaacatttgg acaaggagat gtaaaataat gacagttaaa aatttaaaag gtgtaacagc
     5881 tgcaagcgac caattgatga aagcttttaa agatggtaac gaagaatctt ttagcgcagc
     5941 tatggtaagc ttatctaagg aaattcagga taaaatttta gaagaagcaa cagcaaaaaa
     6001 tcaagatcaa ttagtattaa tgaaccgtgg tcagcgtgta ttaactacac aagaaacaaa
     6061 attctataac gaagtagtga aaaacgaagg ttttgcaggg gtcgaagaat tagtgccagc
     6121 tactgtattt gaacgtgtat ttgaagattt agaacaatct catccactat tgcaaaaaat
     6181 tacttttgtt aacacaactg gtgtaacaga atggattgtg tcacgtggag tcaatccagc
     6241 atggtggggt aaactttgcg aagctgttaa aaaagtttta gataatggct ttgatgtaat
     6301 taacatgaag cagttcaagc tatcaggtta tattcctgta tgtaaggcaa tgcttgattt
     6361 aggtccagta tggttagatc gttatgtccg tactgtttta gtagaatcat tgagaattgc
     6421 attagaacaa gcaattgttg atggtactgg taaagatatg ccagtcggaa tgatgcgtga
     6481 catgagcaaa caaactagcg gagaatatgc tgaaaaaaaa gcagaaccta ttacagcttt
     6541 agatgctgta actatgggcg gtttgatggc gcgactatca aaattcaata tcgaaggtgt
     6601 gaatgatccg atttatcgta atgtgaatcc ttctgatgtg gtcctaattg tgaatccaac
     6661 tgattactgg tctaaagtat tcccagctaa gactgtacta actgctaatg gagaatacgt
     6721 acaagtattg ccagtaccag tttcagattt gcagtcaacg gctgtgccag aaggaaaagc
     6781 agttattggg gtagcctcag attacttcat gggtgtagga tctacactaa aaattgaagc
     6841 ttcagatgaa taccattttg ttgaagacga acgcatttat ctagctaaac aatatgcaaa
     6901 cggtcaacct aaacgtaacg atagtttcat tgtattagat attagcgctt tgggaactac
     6961 tactacaact acaaaaccaa caaccacaac aactacaaca caagcgtagg tgatcagaat
     7021 gaagtatatt ctttgtcagc cggcaatcaa tcggtttaaa tgggagcttg aagtttgttt
     7081 aactaatctg aagaaactag gaatcaaaga tatcgtattg cttttcagca gacacgatga
     7141 tcagattcct attttttttg agaaggaata tggtgttgaa gttcatgtgt acgatgatct
     7201 gcgggacgac aaagagtata ttccttcgat taaaccatat ttatggtgga aatatttaga
     7261 agaagatcat tcgcgtgagg acgaccgata tttctatatc gattcggatg tcattttcaa
     7321 taaaagaatt aatttgcgca aattgccttc taaagatgat gtttggtatt gtagcgactg
     7381 ctgtagttat ctaagtcttg attatattag aagctgtgaa aacggagaaa atattctaaa
     7441 agatatggca aacattgtaa atgttacagt agaatctttg gaaactataa acactaattc
     7501 aggaggcgca cagtgggtta ttaaccgccc taaagcgaat tattggaaaa aggtttatct
     7561 ggattctaat cggctatatc gctaccttag agggcaaaaa acaaatatac aaatctggac
     7621 agccgagatg tgggcacagc tttggaacat gatgtatttc aatattggtc ctaaagttca
     7681 cgaggaatta gacttttgtt ttgctactga tccaatagaa aaagttaaag aagtaaaaat
     7741 cttacacaat gctggagtaa caacaaacga tgaagattta tttttcgaag ggagatacgt
     7801 gacttctacg ccttttgatg aagatttatc atttgtaaac aagaaaaaat gctcttacgc
     7861 atatgttaaa gcaattaagg cggtggttag atgacgcctg aacaagtgac tgaagaattg
     7921 ctaatagctg tgaaggataa tatttacgtt acctggaacg aagaagatga gtcaattaaa
     7981 aagatgatag ctaaaaatgc tgtttatctt caaagtaaag tgagtacaac actttctttt
     8041 tctcctgaaa gcttagaata cggattgcta atcgaaagat gtagatacga ctggaatcgt
     8101 gctttagatg agtttgaaca aaatttcgct agtgagttat taggtttcat tcaacattat
     8161 gcgctacaag aatatattgc aggtgatgtg aatggcgaat aatcgtagac tcgaagaaac
     8221 attcaacgat ggttggttaa agattttgac gcaaactacc aaaagaaatg aactaggaaa
     8281 aaagattggt gtagaagata cagaaatcac ttctttaaaa tttagaaatc tttccatgag
     8341 agatagtgat ataacagcta tggatgcgat gggatcgaaa ttaactaaga aagtaaagac
     8401 tccatttcat ccaatcgcca agaaatttaa taaagatcaa tattttatcg taatcgatag
     8461 tatgcgttac aacgttatct atgccgatta cgataatttt tatatctatt tttatcttga
     8521 aagtgtgggt gaatatggtg attgataatt ctaaagaaaa agaacgttta aataagcaaa
     8581 tttctgctat caaaacttcc ttagaagagc attttggcct caaactcttt caagactccg
     8641 taggcgagga tgagctacct gatgatttta attacttcat tctcgaaaca ggagaaatag
     8701 aaatgatcac tgagccaaaa tatagcgtgg gtcaaaatct atatctaact ttctattcag
     8761 aaaatagaga agatttaaca ggagattcac tagatattat ttcattgatt caaaatcgtt
     8821 cgattcgttt tcagagaatg gatcccaatc atttaaaact agagaaccaa gatcgctata
     8881 tcgatcaatt ggtatttacg tttagacgat tattgaagag tgattgtcat ggctaaaaat
     8941 agttgggagc taaaaataaa tggacatgat gaacttcttg tgcggatgga acgctattca
     9001 agcgagagcg aacgactgat taacgaagca ttgaaatcaa aaggttcggc tattgcagtg
     9061 gataggatta cagaaaaaat tcctgtttct gaagcagatt taagaagagg gcaccaacac
     9121 gcaaaaaata gtcgtccact taagactcaa tacattaatt tgggtttcat cattagacct
     9181 acaagaaaat ttgagtattt aaaatatcct gatttgggga taggtacttc taaaagaaat
     9241 cagccggacg aatttatgag aagaggatta ggtcttgcac ttgatccaat tacagaactt
     9301 ctgattcgtc aattcgataa attaaataaa tagggggaac aacaatggct aaaacaaaaa
     9361 ctgtagtaac aacgttcgat aacgtaagta tcaaacgaat tgcttttaat tttaagaacg
     9421 caggaaatgc aatcgcaacg aattgtaacg gacaattaga tggcgaaaca gaaatgcaaa
     9481 cggtggttaa aaaatgtgga gcgacagaag taaaatcaaa atctaaacca atcaatatga
     9541 cggtaacaat tactgcacat gtaccgatgg aagtttatcg acgtttcaat gggttgaaac
     9601 aagatggacg tattaaacca ggcatttact cttacggtcc tgattccgta ggcgaagatt
     9661 tctcacttgc tgcagagatc gtggatgact tcgaagaaaa tagaaagtta gttggtatgt
     9721 tagcatgcac ttcgaataca ggattaacat tctctattga aaatggtgcg gatgaagtag
     9781 ctgcgttaga actagaaaca aaagttatgc aagatgaatt tggtaaattt tatcatgaag
     9841 caattgttgc agaacttgaa gaagacttaa cagatcaatg gatgacgaat ctatctgctg
     9901 atgtgattaa aaaacttcag ttgtgacaac tacggccact caatcacagt aaaaaaaacg
     9961 gaggtagcga aatgaacgaa gattactcaa aaattgaact aaacgatgga acaattttga
    10021 atttagaacc taaactgaat atcaagaaat tattgatgat caatagagat tttaacacag
    10081 acgagtttgc aaaaatgact gtgggaaaag gatccatgga tatttctgtt attcaaggtg
    10141 caaaggctgt gtatattgct taccgccaag cgaacatgac tgattatatt tcattcgatg
    10201 aatttattga taaatgggat tttgatatgg ctactgccag ctatatttat caattgatga
    10261 tgttcaaaca agcacgagat gcctatcaaa aagaattcga aaaagcaaat aaggaaaaaa
    10321 agcttcaaaa gtaaaaatgc caaagctctt agttgaaacg tgggtcgatg tctattcgat
    10381 gttgaccgac gttttttcta tgccttcaga tttagtttta agcgatatct gtttagatga
    10441 cattttacaa atggcttaca acaagagcgc ttatgaagga tggaagaact acgcaataaa
    10501 tcaatcccaa aaaaattaaa gaaaggaggt aaaaaatggc taaaaagaga acagaagcag
    10561 aagtaacttt catagctaac gatgacggat tgaaatctac gttaaaagaa atcagcgctg
    10621 aattaactaa aaatagagca gaattaaaac tagaacaagc tcaattacaa cagactggtt
    10681 ctgaatcaga caagttagga agtaaattat cttctttaga gaagcagtat gaattacaaa
    10741 gtcaaaaagt tgaagtaact agccaacgtt tagccaatgc gaaaaaatat tatggagaaa
    10801 attccaccga agttcagaaa cttgaaagag aactgattaa tcaacaaaca gcgcaacaac
    10861 gtttgtcaaa cgaaattgat aaaacgagta atgcactagc tcaagcaaaa ggcgaaatac
    10921 agacgtacga gtctacaatg caacagttgg atagtgaaca aaaaaatgtt caagctagtg
    10981 cttctctgat tgaatccgaa tacaaaaaat ggcaagcaac tgctggtcaa tcagcttctg
    11041 aagccgagaa attagcgaaa gcccaagaat atgtttctca acaatctgaa aatgcggaga
    11101 aaacgataga tatcctaaga cgacagttag aagctacaca gtctgaattt ggcgctacat
    11161 ccacagaagc aatgcagatg gaagcgaagc ttaatgatgc tgaacgtgaa tttgaagagt
    11221 taggacaagc tgctaaaaat gtagatacaa ctaacttgga cgatatcgga agcaaaatag
    11281 atatgaataa tttaatggaa gcttctgacg ttttaagcga cattggcgat aagcttacag
    11341 aattagggaa acaagcagtg gactctgcta acagtgtagg tagttcccag agtaagatac
    11401 aagctaattt tggtttgtct aaacaagagg ctgaagaatt aacgaatgta gccagagaca
    11461 tttattataa aggttttgga gaatcgttag atcagtccac agatgcattg attttggtaa
    11521 agcgtaattt aggagattta aataatcaag atttacaaaa tatcacggaa caagctatgg
    11581 tcctagaaaa caccatgggc gctgatatgg atgaaacgtt acgtggtgta aatggcttaa
    11641 tggtcaattt cggcttgagt gctcaagatg caatggattt aatggtttcg ggtactcaaa
    11701 acggtttaga taaaacgcac gaattaggcg acaatatggc agaatatagc caattatgga
    11761 gtcaaatggg atattcagct gatgaaacgt tcggaatgct tcaaaatggt ttagatgcgg
    11821 gtgcttataa ccttgataaa gtcaatgact tagttaagga aatgggaata tcgttaacag
    11881 atggtcgatt tgagcaaaac atggatatgt ttagtgaaag tactagaaaa gcttttgaag
    11941 agtggaaaaa tggcggagga acacaaaaag acgttattaa ttccatgatt caagatttta
    12001 gcaatatgga tggtcaatac gaccaattaa ataaagcttc gacaatttgg tctgcgcttg
    12061 gcgaagataa tgcgatgaaa gttgtccaat ctttaactga tgttaaccat acatttgatg
    12121 atgttagtgg atctgcacaa aaaatgaatg aagattctac tactccgttg caagagttaa
    12181 acgggaaaat agctgaatta aaggattcat tagctcctat aggcaataca atcatagatg
    12241 cactcgaacc agtaattgat tttctaggaa agatggctga tgcgtttaat aatcttccac
    12301 aaccagtaca ggattatgcc gtagcgattg gcggattgac tgctgcattt actttattaa
    12361 tgccaataat agtcggcttc atggctctag gtggtcctac tacattaata ataggagcag
    12421 ttattactgt tattgctgga gttatagcaa ttataaaaaa ctggggcgca attactgact
    12481 ggtttaaggg aatatggagt aaatttactg attggttggg tgatacttgg gaaagtataa
    12541 aagaaggtgc ctcatcagtt tgggatggag ttaaagaaac ctggtctgga tttgtagatt
    12601 gggttcaaga tatttggcaa ggagtttctg attggtttgg agagctatgg agcggattag
    12661 ttgaaggagc ttccaacatc tggcaaggag tccaagagac ttggcaagca ttcgttgatt
    12721 gggtttcaaa tatttggaac ggagtcaaag aagtatggtc gattatttgg gcagacattg
    12781 taggaattgt tcaaatacca tggacattaa taacgtcatt gattcaagct ggcattaata
    12841 ttatcgtggg tatttttgat gtagctggac agttattagg cgcagcttgg caagctgttt
    12901 ggacgcctat ttctgatttc cttaaaaata cttgggatac tatgacacaa tggataagca
    12961 tcgcttggaa tggaattgta actacattcc atactatatt tgatccagta gtggcatggt
    13021 ggaatggtat atggacagct attagtacta cggcttcaaa tatttggaat tcaattagtg
    13081 caacagcttc tagtatttgg aacagtatca agaatacaat cactagcttg gtacaagcag
    13141 ctgctacagt aattcaaaat atttggtcaa ctgtatctag ttggttaggt ggaatttgga
    13201 attcaatcag ctctacagca tcaaatatct ggaatagtgt gactagtagt ataagcaatg
    13261 ctataaacgc agctaaaagt gccattcaaa gtgtttggaa tagtatatct tcgtggatta
    13321 gcggaatttg gaacggtatc aaaaatactg ctttgaatct ttggaatgga attacaagca
    13381 ctattagctc taaagtaaac gatggaaaaa atgcaatttc aagcggttgg tccaatctaa
    13441 caggtattgt ttccgacata ttcaataatg ttaaaagtac aattgctaac atatgggaag
    13501 gcatcaaaaa gactgttagc gctccaattg attggattag agacaaaatc agtggcattt
    13561 ttgataattt gaatatttct ataccacata ttccgttacc ggaatttatc atggaaggca
    13621 gctttaaccc gctaaaaggt caaatccccc atttgcgtgt aaaatggcat gctaaaggag
    13681 gtatctttac taaaccaact ttactaggtg gaatgaacgg tgtcggtgaa gcaggacctg
    13741 aagcagtttt acctttgaaa agatctgttt tgcaagaaat tggtgatcgt atcttgagta
    13801 gcacatcagt ttcatctagg gcacaaacga ttcaacctgt gaataactac gaattcaatt
    13861 tcacaattga tggtaacgca gatgaggtta ctatgaagca aacaactcaa caaatcattg
    13921 atagcattac aaaaattcaa aatgataatg cttcggcatg gcgttaaaca ggagagtatt
    13981 tctcctgttt ttttagtatt aaaaaggatg tgaaaaaatg actgattgta tacattctat
    14041 aatcgatgga tttcctgatt atttgcataa attggcttta gctgaaagac caaccatacc
    14101 ttctccaaaa agacagagag ttgaaacttc tgttttaggt aggttaggtg gcttagtaca
    14161 agattactcg tttgaagata tgtcgtttac attgcactat aactatttag aggatgtgga
    14221 agaccatcaa gcgttcaagc aatcgtttta tatcatgcgt cattggttaa actatgcaaa
    14281 gaaattagaa ttctctgacg atcccaacgt ctattacgtt atccagacta tcgatattgg
    14341 ggatgcagaa aacgatattg ttgaatgggg agagttcgat gtaaatatta ctgcgaaacc
    14401 attcgcaaga gttcaagaag atgtacctat aaccgtagat aaaccacagt catttaactt
    14461 gctgaataat agtttagaag aaagttttcc aaagattatc atcactcctt cagctacttc
    14521 atgccagttc atcttaaatg attatgtgtt tagtttcgaa ggcttagtag caggaactga
    14581 tgtagtcatt gatagtgatt tgatgctttg ctacgaagag caatcggacg gagatatttt
    14641 agatcggtcc aacaaaatga agaccatgca atatccgaca ttgcaagtgg atatcaatca
    14701 ttttaattgc actggtttga gcaaaataca aatttatcgt aacgggttaa ggtaggtgaa
    14761 atagatgatc gataatttaa taactattta cgataaaaat gacgcgaata atttagctga
    14821 acatttatat gatacgcaag gtttaggcgc tttgtcagac tggttaacag ctactgttag
    14881 caataaacta aacggagccg agatatttca gggtacttat tcaataaacg gaactaatgc
    14941 agatttgatt gtagaaggac gtattattca gtgttatgta gatgaaaatc gagcaaaaca
    15001 gcgtctacgg atttattatg caaaaacttc tgtaataggc aatacgatag aagtaaaagc
    15061 tgaacctatt ttcaatgata taagaaaatc ggtgttgaat aaatatgaca gcggaacaga
    15121 aaagatcact gctactcagg catggcaaaa cgcaaaagtt ttagcgaaac cagctatccc
    15181 ttcgcagttt tctttctcgt cattagtaga tacgcttgct aatgtgaaga tagaaaaggc
    15241 gaatttttta gaattctttg gtggaaaaga gggatctatt ctagatcgat ttcatggtga
    15301 gtttctaaaa gataataaca cattacgtca tgaaaaaagt ctaggtacgg atcataaaat
    15361 caaagcgatt tatactaaaa acttaactgg tcttgactta gagattgatg ctcaaagtgt
    15421 tttagttgga gtttatccat tcattagcag ctcttcagaa ggagaagacg agatcactct
    15481 accagaagaa gttattttca cggattacgt ggatgattat cctgctggat atgtttcttt
    15541 tgttgatttt aaagacaaag cgactgatgt agccacatta agggaagctg ctaaagactg
    15601 gttgaaaaca aacatagata aacaaaaacc acaagtgagt ggttcgattg aattagtacc
    15661 attgaggcat caaagaggct atgaaaaatt tgttgatcta gaaaaagttt cgatgggtga
    15721 cggagtagat gtgtatcatc cacagttaaa agtgaatatg tcagcgagaa ttgtggaata
    15781 tacgtttaat gttctaacca attcatacga taaattagtt gtaggaaacg tcaaaacaaa
    15841 cttcttagaa aacacagaga ataatgtcag caatttgatt aatgatgcca ttgatcaatt
    15901 gaaaaacggt ggcgaaatca gtgatttact caatgatatt gtagatcatc aaactgatat
    15961 gattactggt caaaatggtg gttatgtttt attagatcct aaagaagcgc ctagtcgtat
    16021 tttgattatg gacacaccag ataagaatac cgcaaggaat gttttacaaa tcaacaatgc
    16081 tggtattggt ttttctaaaa ctggcattaa tggaacatat gaaacggcat ggacgttaga
    16141 tggcggattc aatgcctcgt ttattacggc tggtgaaata gtaggaatta ctattagagg
    16201 tactacatta attagtgatg gtgctgatta tagaacaagt attgctaatg gcaaaatgac
    16261 ttggtactca aaaaaagtta acaaagatat tatggagcta gaagcacgtg attatgtaag
    16321 tgctgatgcc ggtattgtat catacaccat gaaaactggt ggtggtttca tgattagaaa
    16381 tccacagggt aacttggttt ttagtacgtg ggataatggt aataacagac cgtttctatc
    16441 ttttggtgcg cccaatttca ggtatagcaa tgctagttat gtaacttctg gcgacggtag
    16501 ttctttaagc attaatggta gtgcgggtaa ctcatgggaa tttaaggtag ctggtaggac
    16561 tatgaaattt actagtgatg gtatgctaac gttaccaggt tgtttttttg gttcatggga
    16621 agatgggaaa cttgctaggt ttgaacaatc aacggtacaa gtatataaag attttactgt
    16681 tagaggtact aaaaactcaa ctgtaccaac agaacattat ggacaacgac tattgaacgc
    16741 ttatgaaact ccagaatatt atttcgctga ttatggggaa gccgttacag gtgacaatgg
    16801 taaagttcgt gttgatattg accccatgtt tgctgagaca gtaaatctaa gtcggtatat
    16861 gacacatgtg acacctacag aactagtttt gtgtgctgtt actcatgaag atgttgacca
    16921 tttcatcatt gaaactagta agccaaacgt attggttaga tggaatttag tggcacatcg
    16981 actagggtat gaagatgtta gattaaaaga ggatacagca tatgatagca cagtgcttga
    17041 ccaaaaacgt ttttaaaacg aagacaagga ggtatataaa tggctagcag tttatataat
    17101 ttggctttag atttcagcaa agaattaaac tacaccaaag ctattatggc tcgtcaaggt
    17161 gataaaggga tcacggtgac tgttaaacca tttttaaatg gcctacagat ggatacgagt
    17221 agcggaacat ttactttaaa aggaacaacg ccatctaatc gttacgtaga tagtgtggca
    17281 actagtgtaa ctagtgaaga agtcacattt tctcttgatg gcacatttat gagtgaagca
    17341 ggatattata aacactgcta cgtagaatat agaaaagacg atcaaatttt aacaacgcaa
    17401 gatatcattt ttttctcact aggagtgtct gacatttcgc aaggccaagc cgatgaatac
    17461 gtttcgcaat tggaagagtt gattcgaaag tataacgaaa cttttgatgc ttttatggct
    17521 gaaattaaag gtagagtgga tagcttaaat caacagatta ctgatttaac tggtcaagct
    17581 aaaacgctac aagacaagtt agatgctctg aaagaagaaa tttctaagtt aggtaactta
    17641 caagtgatgt acagtaacag catcgacttt gggggctacg attatagcgg gaatccgaat
    17701 ttaatggcta atataaacgc tgacagcttc tcgcaaggta gtggtgcctt atctgttgta
    17761 gatgatggtg acgaggtagt agtcacgctt gacccaaaca ataaattaga gatattcaaa
    17821 gcgaaaagtc aaccagctct ggtagtagga aaaacatata ccatgagcgt agaaattatg
    17881 ctagaaggcg atttcactgg agaccctagc aagataggcc taagatatat taaaatgcct
    17941 aactgggtat cagagctata tacgcgtaat acattaactg ctactaaggg tgtatggcaa
    18001 aaactaactg gcaccgttaa aattactgct gcgagtgata acgctgaaag ctggcttata
    18061 atgctacaaa ataaagacgc taataacagc ctatccggca aactacgttt gagacacgct
    18121 aaagttgaat acggctcaga agcgacaccg ttccaaccaa acctattggt agaaccttat
    18181 aacatatgcc gcgaatatcc taatgagaat ttgtgcaacc ctacagtgaa gtttcctata
    18241 aaatcaagtg ttgaaacgtt atactcaggc agagtacctg aagatttcgt taaaggagag
    18301 acgtacactg tgaccatagg cgcaacaaaa ccttcaaccc aatctttcgg ggtatattta
    18361 gcgttagaaa atcacgccat attcaaatcg gttgaaggtc tgcctaatac atggacggct
    18421 accctaaaaa tagaccgttt aggagaaaag aaaaacgctg tgtacattta ccaatctcct
    18481 gcgtcaagcg ttggcgcgtg ccagatagac tggctcaaga tcgaaaaagg cgacacacga
    18541 accccgaata ttgagcaata taaataccga ggaatcggca tgcgtgattc aaacaatcca
    18601 aaagattatg tttgggatct agcaccagaa tatgtcgaag ataatttggc cacagatatt
    18661 aaaatttctg aaattactgg taaagcaaac aattataccg atgggaaagt atcggagatt
    18721 aattcgcagt tgactgcttc aattaatgaa gtagacacca cagctaagga tgctcaaaca
    18781 aaagcgaatg ctaatgcgac tgctatagat gaattagaca ataagatcga tgaacgcatt
    18841 aatgatacag ctactaccac attaacagtt acaaacggga ataccggatc agcaaagctt
    18901 tatcgtgaag gaaaaacagt ttctatatat tttgtggctt taaacggaaa acgcagtggt
    18961 ggaaatgatt caacgatact aacaattcca gaaggctatc ggccaccaat tagttttgag
    19021 caactggttg gctcgataga ccgttctact ttgaacagtg ctcagttatc tattggtgca
    19081 gatggagcca ttaaatggcg aagaaactca agttatggat cggattatac ctttgcaatt
    19141 acttacacga tttagaaaag cgtgaatcga tatgaaggca gcatatagac caattgaacc
    19201 ttacggattc gagcaaatca ttgtgaatga tgaagaacat ttaccggaag aatgcacaga
    19261 agtcgaacca ccgattccaa attggaaacc gaaattcaat tactgggagg gaaataaatg
    19321 aaaaacattt ggaaatatgg acgtactggc ggagagtacg caggaaaagt attggacgac
    19381 atgcttgtat ccgttcctta cacagatcag ccaccgcttg aaggaattcg tgctgatggc
    19441 gaaccgttaa caatcgctga tcaaatgttt gatcctaaat tgaatcaatg gattatttta
    19501 gcgaacgcac tagatcacaa cgatttaaac aatctcaaag caatgtatga gtcgttagaa
    19561 aatgagaacg gcgatttaaa acagatcaat gccaaactca tgctaagcga tgtagcaatt
    19621 aaacaggaaa atactgcatt gaaagaaaaa gcggatagtt tagcacaaat caattcaaaa
    19681 atgatgcttg cttcgttaca aaatagcaaa gacatttcag aaattaaaga gcaactaaat
    19741 ccagcttcaa agggaggtga gtagtatgtt tagttttagc gatgtgaaaa tgatgtatga
    19801 ttggggctgt ttcactaacg aacaagtaat ggttttcgtt ccgttgtgca ttactgaaga
    19861 aaaagcagat aaaatcatta gcaaagaaga gagcgcatct taattgatgt gctttttatt
    19921 ttgattcaag gagttgtcac atgattaatt taggggaatg gggagcgata gcaggatcaa
    19981 taaccgctat cgtttctttg attttattag taataaaacc aattactgca tctttctcga
    20041 agattactga gactctttca aaagtaagtc acaatttaga tttgctgact aaagatttag
    20101 aatcgagcaa atcagatcga ttgatgattc atgaagaact aaagaaacac gatgaaagat
    20161 tagatacaca tgcagaaaaa ttggtagaac acacgcaaca aattaaaact ttatttaggg
    20221 agagaagaaa atgaataata aaacgttcga agtactaaaa tggttcgcac tggtaattat
    20281 tcccgcacta gctactttcg tggggttagt tggtaaagcg ctcaattggc agtacacaga
    20341 tatctgtgtt gtcatcatta ctggttttgg cgcgttttta gggagtgtgt tgggtgtatc
    20401 aaatcgaacc tacaaaatgt tctcggctga aagcgaagaa ggaggaaaca aatgaaaaag
    20461 aaaattacta ttactgcgat gagcctgtta acggctcttt ttttattacc cattaatacg
    20521 tttgcttata ctattaatga cgagtataat ttagcgccga atcaaggaga ctccagatta
    20581 gcaattccta acaaaattat tttgcacgaa actggaatag atgcaccagc aagaaacgta
    20641 gccgccaaca tgaaaaataa ttataacgga agcaatcctt atactacaga tgttattggt
    20701 gacggtggga ttgtttaccg tgtgggtgag caaggatatg tttcgtgggg agctggtaac
    20761 gctaatcctt atgcgcctgt acagattgaa ttacagcgca catatgataa agcattgttt
    20821 gaaaaaaact atcgagctta tattgaatat acaagagata gtgcaaaaaa atatggaatt
    20881 ccattgactc ttgatcaagg aacttcttta tttacaaaag gaatcatttc tcatttgtgg
    20941 gtgacaaatt atgtttgggg gaaccacaca gatccatatg gttacttatc gcaaatggga
    21001 gttagcaaag aaaagcttgc ttatgattta gctcatggat ttaccgatga aaatccaaca
    21061 acttctgaaa acaagccagt cattgatcca actagagcag gtgcagcaaa tcctacactg
    21121 acagatggaa caaattacgc ccacattgat cagttcggag aaatcgaaaa cgcaaacttg
    21181 catgtggctg gatggcacat tgctaactat caatacgaat atattttcat tatggattac
    21241 aatactggaa aagaactagc tcgagtaaga gctgatggaa tttatagacc agatgtaaat
    21301 caagcttata atacttctgg aaacgttggt tatcatgtat ctttcaatat gcgtaatttc
    21361 cccaacaaga aagtatacgt catgatgcgt gcaacgaatg atccagaagg gaacactaaa
    21421 ggcggagcac aagattttca tgataagcgt tggtatttga atattccaca acgataaaaa
    21481 aagcccctc
//
LOCUS       DACECZ010000036        21014 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.036, whole genome shotgun sequence.
ACCESSION   DACECZ010000036 DACECZ010000000
VERSION     DACECZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.036"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            157..1923
                     /locus_tag="IWO35_001284"
     CDS             157..1923
                     /locus_tag="IWO35_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ0316349.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            1938..2375
                     /locus_tag="IWO35_001285"
     CDS             1938..2375
                     /locus_tag="IWO35_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0316350.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            2388..3539
                     /locus_tag="IWO35_001286"
     CDS             2388..3539
                     /locus_tag="IWO35_001286"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ0316351.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(3601..4299)
                     /locus_tag="IWO35_001287"
     CDS             complement(3601..4299)
                     /locus_tag="IWO35_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ0316352.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(4292..5644)
                     /locus_tag="IWO35_001288"
     CDS             complement(4292..5644)
                     /locus_tag="IWO35_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ0316353.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(5867..7267)
                     /locus_tag="IWO35_001289"
     CDS             complement(5867..7267)
                     /locus_tag="IWO35_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ0316354.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            7428..8165
                     /locus_tag="IWO35_001290"
     CDS             7428..8165
                     /locus_tag="IWO35_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ0316355.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            8290..8769
                     /locus_tag="IWO35_001291"
     CDS             8290..8769
                     /locus_tag="IWO35_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ0316356.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            8766..9533
                     /locus_tag="IWO35_001292"
     CDS             8766..9533
                     /locus_tag="IWO35_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316357.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            9763..10332
                     /gene="lepB"
                     /locus_tag="IWO35_001293"
     CDS             9763..10332
                     /gene="lepB"
                     /locus_tag="IWO35_001293"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0316358.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(10436..11038)
                     /locus_tag="IWO35_001294"
     CDS             complement(10436..11038)
                     /locus_tag="IWO35_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0316359.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            11144..11613
                     /locus_tag="IWO35_001295"
                     /pseudo
     CDS             11144..11613
                     /locus_tag="IWO35_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            11719..12303
                     /locus_tag="IWO35_001296"
     CDS             11719..12303
                     /locus_tag="IWO35_001296"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ0316360.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            12306..13379
                     /gene="prfA"
                     /locus_tag="IWO35_001297"
     CDS             12306..13379
                     /gene="prfA"
                     /locus_tag="IWO35_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ0316361.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            13372..14211
                     /gene="prmC"
                     /locus_tag="IWO35_001298"
     CDS             13372..14211
                     /gene="prmC"
                     /locus_tag="IWO35_001298"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ0316362.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            14204..14383
                     /locus_tag="IWO35_001299"
     CDS             14204..14383
                     /locus_tag="IWO35_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316363.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            14405..15430
                     /locus_tag="IWO35_001300"
     CDS             14405..15430
                     /locus_tag="IWO35_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ0316364.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            15605..16849
                     /locus_tag="IWO35_001301"
     CDS             15605..16849
                     /locus_tag="IWO35_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ0316365.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            17002..17949
                     /locus_tag="IWO35_001302"
     CDS             17002..17949
                     /locus_tag="IWO35_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0316366.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            18083..18712
                     /gene="upp"
                     /locus_tag="IWO35_001303"
     CDS             18083..18712
                     /gene="upp"
                     /locus_tag="IWO35_001303"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ0316367.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(18851..19093)
                     /locus_tag="IWO35_001304"
     CDS             complement(18851..19093)
                     /locus_tag="IWO35_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316368.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            19214..19286
                     /locus_tag="IWO35_001305"
     tRNA            19214..19286
                     /locus_tag="IWO35_001305"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19247..19249,aa:Thr,seq:cgt)
     gene            complement(19405..20043)
                     /locus_tag="IWO35_001306"
     CDS             complement(19405..20043)
                     /locus_tag="IWO35_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0316369.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(20166..20798)
                     /locus_tag="IWO35_001307"
     CDS             complement(20166..20798)
                     /locus_tag="IWO35_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0316370.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT         7147 a         3723 c         4222 g         5922 t
ORIGIN      
        1 gttttattta aaattgaagt tttctgatac cgaaatcttt ttattttgct gacatactca
       61 aacctttggc acaaagtttc gtgccaaagt acgactattt ctctttttag taagcggata
      121 caattagagt atcaaaagga gagagggaga gacaaaatgg aacggacgat tcccaaaaac
      181 aacatttcgc taaaagcgag ggtacaaaaa ttaggcagta ctttatcaag catggtcatg
      241 ccaaatattg gagcactgat tgcctggggt gtattaacgg ctctatttat cccagatggt
      301 tatttaccaa atgaggcttt cgctaccatg gtaagcccaa tgctaactta tctgattcct
      361 ttattgatcg gttatactgg cggaaaagta attgctggtg accgcggatc agttgttggg
      421 gcaattgcaa cgatgggtgt gattgtcgga acagatattc caatgatgct aggcgcgatg
      481 atcatgggac ctcttggtgg atatgccatc aagaaatttg atcagctatt ccaaaaacgg
      541 atcaaatccg gttttgaaat gctagtcaat aatttttcag caggtcttat cggttttgga
      601 ttagcgttat taggtttttc tgcgattggt ccagttgtcg atgcattgac tcaagcaatg
      661 gcaaaaggtg tagagatcat tttatctgcc catttgattc cattgacaag tatttttatt
      721 gaaccagcaa aaatcttatt tttgaataat gcaatcaatc atgggatttt gacaccattg
      781 ggaactgaac aagtagcaga ggctggaaaa tctattctat tcttgcttga agcaaatcct
      841 ggtccaggac ttggcgtatt acttgctttt actttgtttg gcaaaggagc agcaaaatct
      901 tcagcaccag gagccatgat cattcacttt ttaggcggga tccatgaaat ctattttcct
      961 tacgttatga tgaaaccgtt attattctta tcagttatag gcggaggaat gtcaggaagt
     1021 ttcattttcc aattgatgga tgctggacta agagcgccag cttctccagg gtcgatcatt
     1081 gcgattctag cgatgacacc gttcaattct taccttcctg ttattttggg cgtgattgtt
     1141 gccgcagctg tttcgtttgg tatttctgca gtaattttga aagcggatgc gaaagataca
     1201 aatgaagact tcgaaaaatc tgttcaagag acaaaggcag ctaagcaagc ttccaaaggt
     1261 gcccaaggta ttcctcaagg agctacattc tctggcgtac aaaaaatcat ttttgcttgt
     1321 gatgcaggaa tgggatcaag tgcgatgggc gcttcgatct tgagaaaaaa agtacaggaa
     1381 gcagacttgc ctcaaacggt gaccaatcaa gcaatcagcc aactgacaga tgactcgaat
     1441 acattgatcg ttactcaggt cgaactacaa gaaagagcaa aacaaaaagc accgagcgcg
     1501 cgttttgtct cagtagaaaa ctttttgaat tcacctcgat atgatgaaat cattcaagaa
     1561 ttaagcagca agcagaaaac atcagtttct gaaattgaga atgagccagc agaagagttt
     1621 ctttcagcaa acatcaaaca aatcaaagag attctacttg tccacgatga tcgttccgga
     1681 tcagcaacaa tggggatgac cgtgctcaaa gatatattag acaagaacca tttaactatc
     1741 ccgttaagaa aagtcaatat ccaagattta aaagatgaac ctgattcact gatcgtagcc
     1801 aaagaaagtt tagcggaaaa agtacagatg gctgcacctc aatccattca tttatcagtc
     1861 aacagcatgg tcaatccgca aaaatacgaa tcgattgcac aatatttgaa acagacagct
     1921 taagggagga aaacagcatg caaagtttgc cattcgacat gatcgagctc aatcgatctt
     1981 ttaaaaccaa agaagaagcg atccgctatt gcggtcagat gcttgtagaa gcagggtgcg
     2041 ttgatgagga atatatcgat gccatggtag atagagacaa aatgttatct gtttatatgg
     2101 ggaattttat cgcgattcct catggaacag atgaagcaaa aaaatatgtg aaaaaatccg
     2161 gcatctgtgt cgttcaagta ccagatggtg tagattttgg taccgaagaa gaagaaaaga
     2221 ttgcgactgt tttgttcggt attgcaggag tcggagatga gcatttgcaa cttgtacagc
     2281 aaatcgcact ttattgcagt gatatggaca atgttgtcca gctagcagac gcgctcagca
     2341 aagaagaagt aacggaaaat ctagcaattg cttaaggaga gagaaaaatg agagcagtac
     2401 attttggtgc aggaaatatc ggtcgaggat ttattggaga ggttttggca gctaatggat
     2461 ttgagattac tttcgtagat gtgaatcaca cgatcattga tgctttaaat gaaagaaaag
     2521 aatacgaaat cgaattagca gatgaatcaa aagaaaaaat cactgtaaaa aatgtcaaag
     2581 gaatcaacaa tcaaacaaat ccagaaaaag taatccaaga aatcgctgaa gcagatttag
     2641 ttactaccgc cattggcccg aatatcttac cttttattgc tgagttgatt gcaaaaggta
     2701 tccaagaaag agaaaaggaa ggaagcacaa catctttaga tatcattgcc tgtgaaaata
     2761 tgattggcgg ttcagcattt ctagaaaaag aagtgtataa atatcttccg gaaacatcat
     2821 ttactgacaa atatatcggc tttccaaatg cagcagtcga tcgaatcgtc ccattacaac
     2881 accatgaaga tcctttattc gtccaagtgg aacctttcaa agaatgggtc attgacgata
     2941 gccaaagaaa aaataaaaaa atccaattaa aaggggtttt atatgtagat gatctagaac
     3001 catatatcga acgcaaacta tttagtgtca atactggtca tgcgactgtc gcttatacag
     3061 gtgccttgtt aggttaccag accattgatg aagcaatgca agatgcgtta gttgtggcac
     3121 aattaaaatc agttttgcag gaaacaggaa gtttgctgat tgccaaatgg gggtttgatg
     3181 cagagcagca tcatgcgtat attgaaaaaa tcattcatcg cttccaaaac aaaaatatct
     3241 ctgatgcgat cacacgtgtc gctcggacac cattacgtaa gttaggttat caagaaagat
     3301 ttacacgtcc agtgagagaa ctacaagaac ataacttgac ttgcccacat ttgacggcaa
     3361 caatggggat tatttttaat tactatgatc ctgaagatga acaaagccgt caattgcatg
     3421 aaatgaagat ccatgaaaac ttggaacaat tgattcaaga agtcacagga atcaatgatc
     3481 cgaaaacaat cgggaatatc aagcaaaacg tgaaccgtta tgccaagcaa gtcgcttaaa
     3541 ataggatacg aaaaagggga atgtgacgaa atttttgtca cattcccctt tttcgaataa
     3601 ctaggctgtt tgtccttcaa gatagggcag atccaattct ttaccgtaac gttgttctaa
     3661 agctaggcgg atcaaacgag ctgccaggtt ctcatttctt gcaaggatag gtccatggaa
     3721 ataagaaccg aagacatttc ggtaaatgac accttcagat tggtcttcac cattatttcc
     3781 ttgaccttgt accactttcc ccaaaggtcg ttcaccttca cctaagaaag tccgaccatt
     3841 gtggttttca aatccataat aagtttcatt gaattcttcg ttatggataa cgatatcacc
     3901 gataaaccgg ttattttcct gacttaacgt gtagtgatcc agtgcactga ttccttggat
     3961 cttttctcca ttagctccca tgtagtagtg acctagcaat tggtagccac cacaaatggc
     4021 taacatgacg ccatcatttt cgatatagtt gatcaaagaa tctttctttt tctggatgtc
     4081 ttttgaaatg attaattgct caaagtcttg accaccgcca aagaaaacca agtcatattt
     4141 atcagcatca aaaggttcat gtatactaac gatctccgta tggcaagaaa cccccatttt
     4201 ttctgctata tatttcaaca tcaaaaggtt tccgttatca ccatatgtgt tcatcaaatt
     4261 tccataaagg tgggcgatat tgagttcata attagccacg gttcattcct cctttgataa
     4321 atccttgagc agctagttct ttacgaagct gcaatacagc agtgtaagta gctaaaatat
     4381 agacatgctc agttggcaat tctttgattt tttcgattac ttctggtaat tcttttgttt
     4441 ctgtcaattt attttctgga atcccggcaa cttttagacg taaagtcata tctgtatgac
     4501 ggtctcctcc agaaataaca gctgggatat ccatatcggc aaagttttca aaattaccat
     4561 cccaaatcca gctgacatcg attccgtctg cgtaattggc atttaataaa gccactaaag
     4621 aaaatggata aggagctaga ccaatcatat cgattacttg attgatacct acaggatttt
     4681 tgacaagtac taatgtacat tttttcccat tgatattgat gacttcttgt cggccgaata
     4741 ctttctcgtc atatcctaaa ccttgtttga ttttatcagg atcgacattg aagtactctg
     4801 ccactgcagt agctgctaaa gcattgtaga cattatacat accgccaact tcgattccgt
     4861 attcatgacc atcgatcacg aaatctgctg acacattgtc catccgtacc atatcagtta
     4921 atcgataatc caattcagga cgtttgaagt cacaatttgg acagtaatat ttccctaagt
     4981 ttgcataagt gatcatttta taatgcaaga tgtgatgaca atttggacat aagacaccgt
     5041 ctgtattgta atgagccatt tgttcacgat cttcttcatg gtcaaagcca taatattttc
     5101 taggattgac tgtttcgata gaattgaaga ttggtgaatc accgttactg ataatcgttg
     5161 catttggtga ctttgcggca ccgtctacaa tcattttata ggtcgtatag atttctccat
     5221 accgatccat ttgatcacgg aaaatatttg taaataagaa cagttctggt ttcatatatt
     5281 ctgtgacttt gctcaaactt gcttcatcta tttctaggac agcaaacttt ttctgtcctt
     5341 tttttggttt tgcctgcaag aaagtcgata caattccctg taccatattc gcaccagtag
     5401 ggttggttag aacttcatcg aattgctggc gcaaaatatt gactgttaaa gcggtagtca
     5461 atgttttacc gtttgttccg gtgacaacga cgacttggta gtcttttgcc aaactatcta
     5521 gtatatgtgg atcgactttc agcgctagtt tacctggtaa acttgatccg cctttaaaaa
     5581 aagtttttaa tgcccattgc gacgtttttc caactgcaat cgcaaaatga ctgcgtattc
     5641 ccattatgta acctccaaaa acgatttgct gacgaataaa ttcaactaat cataccatat
     5701 gttgaaaaaa aggttaaatt gcttttctta ttcttaagaa aaggttacgg ctgaaagtgc
     5761 atgaaatcgg acaaaatcgc atgatttaac aaaaaaacag cttgaaacaa aatgattgtt
     5821 tcaagctgct gtgattttat tattccctct tacctatatt cccattttaa ccgataataa
     5881 ttttctcgct tggatattca tagcctagat ctcgtgtttc ttttggcacg aataacatca
     5941 atgtatatag cattccgatc cgcccaataa acatcagtgt agcaatcgtg attttgccca
     6001 caacagacaa gtctccggta atccctaaag ataaaccagt tgttccaaac gcagaagtta
     6061 cttcgatgat gatcgagatc agcgtttgtt cttctgtagc agataaaaaa acgatacaaa
     6121 agaaacacat accaagagaa agcataaaga caacgactga ttttcgcaca tcatcttgat
     6181 cgattctccg tccaaaaata ttgatgttat cttcactttt gaggaaggaa tatagataca
     6241 aaccgatgat tgctacagta gttgttcgaa tcccgccgcc tacagaacta ggactgcatc
     6301 cgataaacat cagtaaagaa aaaatgatca gtgttgtgat ttgaaaatca cccaaatcgt
     6361 gaatctgcag accagcatta cgagtcgtaa tcgaataaaa catcgaattg atccatttga
     6421 cactcatatt ggaatcttgg aataaatggt ccttttccaa tagataaatc aagacagttc
     6481 ccgaaacaaa caagataaca aaagctaaca cagctaattt ggtaaacaag gaaaaacgaa
     6541 acggaagctt cgcattggaa cgtttgtata atagccattc ccggcactcc atcaatacag
     6601 gaaaaccaat tcctccgaca aaaatcaaaa acatgatcag gatcaagaaa aaatagtcat
     6661 gtgcaaaggg cttgatggaa tccccagtga cgtcaaaccc agagttcgtg acagccgata
     6721 tcgcctgata aaaaccgtaa aaaacggcat cacgccaacg atcaaaatag cctctgtaat
     6781 aaaaataaat agaaaagaat gtcccaaaaa gtaattgaaa ccagattaaa atagcaaaag
     6841 tgatccgaat cagtcggacg ataccagaca aacgcggttg attcatatcc gtcatgatca
     6901 gctgtcgctg tttcagtgtg atccgtcgtt tagaaaaaat gacaaaagca gtagaaatca
     6961 tcatgatccc caaaccacca acttgaaata atatttccag cagtataata ccgttatcat
     7021 tgaacacaga attgatatca aaagtcgata gtccagtcac actcactgta ctgattgcca
     7081 tgaacagcat gtccacaaaa gaaacatggc tatctggttc gcgaaaaatc ggcatataaa
     7141 atagaacgag tgataagacc gtcatcaata tataataaaa aacgatgatt tggatcgatg
     7201 agaaatgatt ggcagcaaac ttctgtcctc gccgctgcag ccttcgaaag aataaatcaa
     7261 catgcacagt actatcctcc tgacaatagt cgtttctacc agtatacaag gggaattagg
     7321 aatactcaaa gaaattatca aacagataac aatacagctt tcgattaaaa tagaaatggc
     7381 ttataataag gcaaatccta gaaaaaagaa ggaggaggaa taacaaattg attaatgctc
     7441 aacaaatcgt agaaaaaatg aaaaatcaaa aaataaatta tgatacagtt ctgaaaaaaa
     7501 tgatcatgca atgggagaaa gaagaagtaa agcctaagat attaatacat acatgctgtg
     7561 ctccttgcag tacgcacaca ttggaattca tgtctcaata tgcagagatc acgttatttt
     7621 tttcaaattc gaatattcat cctaaaagtg agtatttgcg aagattacat gaacaaaaac
     7681 gatttgtaga ccaatttaat gagaaaactg accatcatgt tgcgctaatc gttgatgaat
     7741 accgtccaaa tgaattcacc aaaatggttt taacaaacca tcttgaaaac gaaaaagaag
     7801 gtggtgctcg ctgcagtgca tgcttcaata tgcgattaga tcttgttgcg caaaaagcgc
     7861 aagaactagg atacgattac tttggaagtg ccctgaccat ctcgccaaag aaaaatgcag
     7921 ctttgatcaa tcagatcgga atggatatcc aaaagattta cgggacacaa tacttgccaa
     7981 gtgatttcaa gaaaaacaaa ggctatgaac gctctttgca aatgtgcaaa gagtatgata
     8041 tttatcgcca atgttattgt ggttgcgtgt tcgctgccaa gcagcaagga tgcgatctga
     8101 aagaaatcaa tcgggaagca aaagcttact taaaaacatc aaccgtagtt tttgaggaaa
     8161 attaaagaaa tctttcggaa aaacgtaaga aaaaaataaa gaatttatgg tagaaataag
     8221 gtagactgta taatttgtcc gtagtaaagt aaggaaacaa gaataaattt caatgaagga
     8281 agagataaga tgcgaaaagt agcacctatt ttgaagccaa tcgctgtatt aacaatctcg
     8341 acgattgtcg cgactactac aggtattgtg aatagttcca gctaccgtgc accaagttca
     8401 agttctagta ctgtagaaga aacaacacca actacttcag tctcgacatc tgaaccaaca
     8461 acaacggaaa gtttgaatga aaccactacg acaagtacat gggacgatac aacaactagt
     8521 tcgtcgtatg atgagacata tacaactgac agctcaagcg aaacaagtaa tacggtacca
     8581 gatggcactc catcggattc aaccaatcca agtacaagtc cggatacaag tgctagctcc
     8641 gaaacgatac caagcagtac gactgtagat gataacacgc aaaatgatga tgcagcacgt
     8701 acagcacagc aaaaccaaga ttcaggaaat acccaaacgt ataaccagca agcaggtgat
     8761 tctcaatgac atttttacta gaccttatca acggggtgca taaattcctt ggctatttag
     8821 acatcagtcc taaatatctt aaccgtggat atacgatttt aagtgtgatt cccacgcttt
     8881 atttattgag aattgtttat ggactttggc aaaaccaaaa ctatcttcaa tttttcttat
     8941 atggacttgc atttctagtg cttgtttatt ttactgtttt aaatgtattt tattactttt
     9001 tagataaaaa cacgaaagcc gatgtcactc agctttttgt gaagtattta cctgatgaag
     9061 cgttcaacat ccagacagaa gaatcgatag ataatggaaa ttcgatcgat aaagtgaata
     9121 cacaagaggt catggtttct tatgacgaag attatcaatt gaaattagcc gagaatatgc
     9181 ggtatttgat tggaaatggt gagatcaaaa caaatgaact cggtcgcatg gatggatttc
     9241 tggtcgatag aaatacattg tatccttatt actatgtcaa gaaaacagga gagaaagagt
     9301 atgttctaaa tattggacga agctactcgg acatggaaaa agtagggacc attcgacttg
     9361 actcaacaga agacacattg gaacctgtcg gattgttcat cgtcggcggt gattttgtca
     9421 aagacgggtt acgctatcat gagccttatc gtctgaaatt gattgcaaaa aaggcacagt
     9481 cgcaggaaga accagtggtt tatagtcgta gccaaagaag aagacattca taatgtgatg
     9541 aaacgccgtg tatcagtggt tgcgataaaa acgtttattc ggaataaggg ggatgtgaca
     9601 cgtcttttgt cacgttcctt ttttatataa cgagaaagat aagacgaaca catcacttat
     9661 tctttccttt catttataaa cattgtagaa taaagaaagt acaacgaagt attgtgaaaa
     9721 aacgtaataa ataacgagga aaatacaagt aggaggtaag ctgtgaaaga atttttgaaa
     9781 aattggggga ttttgatcct tgtattagcg gctattttat tagctagagt gtacgtgttt
     9841 acaccagtta ctgtaaacgg gcattcgatg gaccctacat taagtgatgg acaacgattg
     9901 atttcatcta agatctcaaa ctatgaacga atggatatta ttactacaaa ggaaccgggg
     9961 gatgaagaac ggatgattgt taaacggatc atcggtatgc ctggagatac agtcaagatg
    10021 gaaaatgacc agttgaccat taatggcaaa aagtatgatg aaccttatct ggatgagttc
    10081 aaaaaagaat tttcagaaga taagttacaa ggagaatatg cttatagctc aggattccaa
    10141 gcacaagcag aaagttcatc cacctttacg agtgactttg aatatactgt gccaaaagga
    10201 aagtatcttg ttctaggcga taaccgcttg atttcaaaag acagccgaat gtttgggtta
    10261 gtcgacaaag atatgatcca aggaaaagtt gttttccgtt attggcctct ttcagaaatt
    10321 aaaatcatgt gatgaaaaaa taaaaagaag ctgtgacatg ttttttgtca cagcttcttt
    10381 ttccgaaaat tccttcttat ttcttggagt tgctcacttt ttttgcaatc tcattttaag
    10441 ctttttggct gatggataag aaaagcgaag cgtttttgtt cataaatagc ttttccccac
    10501 gatggaaggt atgactagta gcagagacaa agcccgtttt ttccaatcga ttattcaatt
    10561 cttcttggct aaagccatta tggactttcg gatgactaat ttgttcattc ttgtcaaagt
    10621 ctacgatgat caattgaccg ccaggagcta aaatttcata aagttttgta agaatattct
    10681 ccgtatcagg aatgtgtaaa agtacaagtg atagaaggat gagattagct tggacagcag
    10741 gaaattcgac agaagcatca gcgtggatcg ttcggacatt tttcagatct gctgcttgga
    10801 tcttttcttc tgccattttc agcatagttt ctgaagcatc ggcaatgatc agttctttga
    10861 atctttccgc aagcggtaaa ctaacaagcc cagttccgcc tccgtaatcc agcaggactt
    10921 tttggtggct gtcgcgcggt agataagtag ttagttcttc gcgaataatc ttggctaatg
    10981 cttgacggtc aggggaatca taatggttag cgatggaatc aaaaatattt tttgtcatca
    11041 aatagctcct tacgtaaaac tttgaaattt taacttatta tagcacgtga aaagaaagaa
    11101 agcatgctat actcaattca aaaagggaga atctagaaag aatatgccga tatcgtttgt
    11161 aaaagataga gaggaaaaag gaaagtgtgt ccgagaaata ttactagact tgcctgagtg
    11221 gtttgggctt cctgaatcaa cggaaaaata tatcgaggaa tcaagtaaac tacctttatg
    11281 gtgtgaaaaa agaaaagaag aatatttagg ctttatcact ctttcacaga caagtgaaga
    11341 tactgccgaa atctattcta ttgtatggga ataaaaaaag actatcatca ccaaggatta
    11401 ggaacaaaac tttttaaaga agcagaaggt tacgcagcaa aacattacaa atatttgcaa
    11461 gtgaagactg tagacgaggg gcactactct atatatgatc aaactatttg tttctacgaa
    11521 tcattaggat tttcaaggtt ggaagttttt ccaaatttat gggatgaatg gaacccatgt
    11581 ttagttcttg tcaaaaaatt ggaacaaaag tgagcaatag atttaatact aatctgtgct
    11641 tgatgaaaat tctcactgta aaaaatgctg aaaatcaagt ataataccat aggaacaaac
    11701 tggaaggtga aaaagagcat ggcacaacta ttttttaagt atggcgcaat gaatagcggc
    11761 aagacgatcg agatcctaaa agtcgcacat aattatgaag aacaaaataa gccagttgtc
    11821 ttaatgacaa gcggtattga tacaagagat ggtttaggcg ttgtttcaag ccgaatcgga
    11881 ctgaaacgta atgcgatgcc gatatttgaa gaaacagacg tatttgacgt aattcagaaa
    11941 atggaaagca aaccctattg tgtgctgatc gatgaagcgc aattcttaaa aaagaaacat
    12001 gtcattgctt ttacgagaat tgtggacgaa ttggatattc cggttatggc atttggtttg
    12061 aaaaatgatt ttcgaaacga gttatttgaa ggatcaaaat atttattgct ttatgcagac
    12121 aaaatcgaag aaatgaaaac aatctgttgg ttctgccaca aaaaagcaat catgaattta
    12181 cattatattg atggaaaccc tgtttatgaa ggcgatcaag tgcaaattgg cggaaatgaa
    12241 gcttattacc ccgtttgtcg aaaacattac tttcacccat caatgattac agaaggagaa
    12301 taagcatgta cgatcaatta caagcaattg aagaccgtta tgaagagctt ggagaacttt
    12361 taagtgaccc agaagtgatt tctgatacaa agcgttttat gcagctttca aaagaagaag
    12421 caaacacgcg cgaaacagtc gaagtttatc gtcgttataa agaagtagta caaggaatca
    12481 ccgatacaga agaactgctt ggtgaaaaac tggatgatga aatggctgaa ctagcaaaag
    12541 aagaattatc tgaattaaaa aaagaaaaag aagtcttaga agaaaaaatc aagatcttgc
    12601 ttttaccaaa agatccaaat gacgataaaa atatcatcat ggaaatccgt ggagcagcag
    12661 gcggagacga agcagcatta ttcgctggcg atcttttcaa tatgtaccaa aagtatgctg
    12721 aagcacaagg ctggaaaaca gaagtgatgg aagctaacgt taccggaatc ggtggatata
    12781 aagaagtgat catgatgatc accggagaca acgtatattc gaaattaaaa tatgaaagtg
    12841 gtgcgcaccg cgtccagcgt gttccttcaa ctgaatcgca agggcgtatc catacttcca
    12901 ccgcaacagt tgtcgtgatg ccagaagctg aagaagtaga gatcgatatt gaagacaaag
    12961 atatccgtac agatatctat catgcttcag gtgctggcgg acagcacgtc aataaaacag
    13021 catctgctgt acgtttgact cacttaccaa cagggatcgt tgttgctatg caagatgaac
    13081 gttctcaaat caagaaccgt gaaaaagcaa tgaaaatctt gcgtgctcgt gtgtacgata
    13141 agatccagca agaagcacaa agcgagtatg atgccaatcg aaaatcagca gtcggaactg
    13201 gggatcgttc agaacggatc cggacgtata atttcccgca aaatcgtgtg actgatcatc
    13261 gtatcggatt aactatccaa aaattagatc agattttagc agggaaatta gatgagatcg
    13321 ttgatgcatt gatcatgtat gatcaaacat caaaactgga agctatgcaa aatgggtaaa
    13381 atgacctatc gagaagtcct gtcacgggct tcttcttttt tagaggatca tgggaaagaa
    13441 gcatatagta tccaattttt atttttggaa cgaaaacaat ggaaaaaact tgattggctc
    13501 cttcatatga acgaagagat ttcagaagaa gaacagcggt tgatcgaaac cgatctgcga
    13561 cttctgcttg cagatcatcc acctcagtat ttgctaggat atgcagattt ttatgatcat
    13621 cggttaaagg taactgaagc aacactgatc cctcgaccag aaacagagga gttagtcgaa
    13681 tggtgtttag acgagacgcc tggtgtaccg ttagaagtga tagatattgg gacagggact
    13741 ggagcaattg ctattagttt gaaagcagca agaaaaaatt ggcatatatc tgctgtggat
    13801 ctttcagaag aagcattaga agtagcgaaa gaaaatgctc agaaagaagg aacgaaaatc
    13861 agtttttatc atggagatac attaggacca gtaatggatc agcaatttga tgtgattatt
    13921 tctaatccgc cgtacatcag tcggaatgaa tgggaactga tggatgaaag tgtccggtca
    13981 ttcgaaccta aaatggccct ttttgctgag aacgatggat tagctattta tgaaaaaata
    14041 gcagttgaag caccaagcgt actaaaatca aatggaaaga tttttttaga aatcggattt
    14101 cgccaaggtg aagcagtaaa aaaaatattt cagcaagcat ttccagataa aaaagtggcg
    14161 ataaaaaagg atctttttgg caatgaacga atgatccaag tttatggttg aagttgtgga
    14221 tgaaaaattt tttatttgtt tttttattat aaaaaaacac ttatttgaca aggatgtaaa
    14281 cgactttttg gtttatcaac aagtttatcc actattgaca aatagattat ccacaaacag
    14341 tggaaaactt gaggataaac tcagacaaaa taagaatgta tgaaaaaggg gttgtggata
    14401 aactgtggaa acgaaacgat atgatgaaac agaattgcaa gaggcagccg aagcactgaa
    14461 agaaggagaa cttgttgctt ttcctaccga aacagtatac ggattgggcg ccaacgctct
    14521 tttaccaaac gctgtcaaga aagtgttttc tgtaaaaggg agaccgcaag ataatccatt
    14581 aatcgtccat gtcgcctctt ttgaacaagt aaaagagtat gtggacaact ttcatccggc
    14641 agcagagaag attgtgaaga acttctggcc tggaccatta actttgatat ttaagatcaa
    14701 aaaagacact ttgccctcag ttgtgacggg aggattatcc acagctgcat ttcgtatacc
    14761 ggataacaaa aaaacactag aagtaatcga actttccggt gttccgcttg tcggtccaag
    14821 tgcaaatacg tcgggaaaac caagtccaac gactgcagat catgtatatc atgatctaca
    14881 aggaaaaatt accgggatta ttgatgatgg agctacgcga attggtgtag aatccactgt
    14941 tcttgattta agtgatccaa cagccatgcc tatgatttta cgaccaggtg cagtaacgaa
    15001 agaacaaatc gaagcagtca tcgaatctcc tgttgctatt gatcagcatc ttgtaaaaga
    15061 aaatgaaaca ccaaaagccc ctgggatgaa atacaaacac tactcacccg atacacgtgt
    15121 tctgatggtc agagatggcg attggtcaac tgcagttcaa tgggcaaaaa ataagaagat
    15181 ccgtgcaggt gtcatagcaa gccccgaaat tgcggatcaa gtgcggacag atactgcagc
    15241 tgtttatatg tataacgaca actcagtaga agcggcggct aaaggattgt ttgcaggtct
    15301 aagaggatta gatgaaccaa cacttggatt agatttgatt tttgtacaag tgtatccaga
    15361 aactggattg gggaatgctt acatgaatcg cttgaaaaaa gcagcaggac aaaattattt
    15421 tgaaaaatga tcaattggtt attgtcgcat tcttttagta tgatacaatg ctatcaagta
    15481 aatcttctat cagtgaacga aaaactaagc gtaactaatc gtaatagaag agatacgaat
    15541 atttggcaat ttgtttttga tgggaaaaat aaaattgtag gaaaataaaa aggagctgtt
    15601 gtttgtggta gattacaaaa cgtttgaccc tgatctttgg gctgcgatcg ccaaagaaga
    15661 agaaagacaa gaacataatt tggagctgat tgcctctgaa aactttgtct ccgaagcagt
    15721 gatggctgca caaggaagta tcttgacaaa taaatacgca gaaggttatc cagggcatcg
    15781 ctattatggt gggtgtgaat ttgtcgatat cgttgaaaat ttagcgattg accgtgcaaa
    15841 agaattattc ggagcgaaat ttgcgaacgt ccagcctcac tccggctctc aagccaacac
    15901 ggctgcttat ctcgctttgg tagaaccagg ggacacgatc ttaggaatgg atctttccgc
    15961 aggcggacat ctaacacatg gatcaccagt caacttcagt ggcaaaacct atcactttgt
    16021 tgcttacggt gttgatccga caacagaagt tattgattat aatgtggtac gtattttagc
    16081 tagaaaacat caaccaaaat tgatcgtagc aggtgccagt gcttacggac gaacgatcga
    16141 ctttgcgaaa ttcagagaaa tcgcagatga agtaggcgca aaactgatgg tagatatggc
    16201 acatatcgca ggattagtag cagcaggact tcatcctaat ccagttcctt atgcagatat
    16261 cacaacgact accactcata aaacattgcg tggccctcga ggcggcatga ttttgacaaa
    16321 cgatgaagca ttagcaaaaa aaataaatag tgcggtattt cctggtattc aaggcggccc
    16381 tctagagcat gtgattgcgg gtaaagcagt agcattcaaa gaagcattag atcctgcatt
    16441 taaagaatat agtgaacaaa tcatcgccaa tgcaaaagca atggtcaaag ttttcaatca
    16501 agcaattgga accagagtca tttcaggggc gacagataat catctgatgt tgatcgatgt
    16561 tcgcgagtta ggtataaatg gaaaagaagc agagagcatt ttggacagtg tgaatatcac
    16621 tgtaaataaa aactcgattc cttttgaaac actaagtcca ttcaaaacaa gcggtattcg
    16681 aatcggcacg ccagcaatca ctactcgcgg gttcaaagaa gaagatgctg taaaagtggc
    16741 tgaactagtt gtcaaagcat tacaagcaaa agatgataac gctcaactag acgaagtcaa
    16801 aacaggtgta cgcgaattaa ctgaaaaatt tccgttgcat aaaaaataaa aatttaaatt
    16861 ccattagtaa acagaaaagt tcaatgttgg gcttttctgt ttattttttg ggaaaacgga
    16921 ttctttgaca atatcggtga tgctctctag tatatgaggt gtaattttag tcttcacact
    16981 aaatctcagg gaggaaaagt catgactgat cgtatctcgt tagaaaagga agcagtagca
    17041 ttcagtgatg caaatgcgcc tcatccgcgt atctatgaac tagcaccgga agaaggaaga
    17101 gttttactag aagaagtaca agaatcacct gtgaacaaat attccgtcga tattgaagac
    17161 ttgatggtgg atactggcga atggggaaaa ctcaatattc gtctcgtacg accagaaggg
    17221 aatatggata aattaccagt tattttttat attcatggag caggctgggt ttttggcagt
    17281 gctcaaaccc atgataaatt gatcagagaa cttgcggttc gtacgaattc cgtcgttgtc
    17341 tttccagaat atagtcgatc accagaagca aaatatccaa cagcaattga acaaaattat
    17401 gctgtgttgc aacagctgaa taaattagcc aagtcaaaaa atctggatct cgagaatcta
    17461 atcgttgcag gagattcagt aggtggtaac atggctacag tcatgacgat cttaacaaaa
    17521 gaacgaaagg gcctcccaat atccaagcaa ttgctgtatt atccagtcac aaacgctgat
    17581 tttgatacag attcctatca tgaattcgga gaaaactatt ttttgacaag agaaggaatg
    17641 atgtggtttt gggaccaata tacgacgaat gaagaagagc gtgctgaaat cacagcttcg
    17701 cctttgagag caaatataaa tgaactaaat gatcttcctt cagcaatgat tttaacggga
    17761 gaagcggatg tattaagaga tgaaggtgag gcttatgcgc gcaaacttcg tgatgcaggg
    17821 gttcctgtta cacaagtacg cttccaagga atcatccatg attttgtcat ggtaaatgct
    17881 atggatcaga caaaggcaac gcgtgcagca atggcattat ctacttcttg gatcaagaaa
    17941 aaagaataag ctttgcttgt ccgcaggaaa acttcttttc aaaaacggat tttcttagga
    18001 aaaatggcga gtttgactag tcaatcactt gattttgttt tacaattaaa aagaaacgac
    18061 agaacaaagg agagcttaaa aaatgggcaa atttcaagtg atcgatcatc cattgattca
    18121 acacaaacta actattattc gggacaaaaa ctgcggaaca aaggtttttc gtgaagtagt
    18181 cgatgaaatc gctatgttga tggcatatga agtatcacgg gatatgccat tggaagacat
    18241 cgtgatcgaa acaccgatca ctgaaacaac acaaaaaacg ttgtctggta aaaaagtagc
    18301 gattattcca atcttgcgcg caggtattgg aatggttgac ggtatcttgg aattgattcc
    18361 agctgctaaa gtgggacata tcggtcttta tcgagatgaa gaaacattgg aaccacatga
    18421 atatttcgtc aaacttccag aagacattga tgcacgtcag ttgtttgtag tagatccaat
    18481 gttggcaaca ggcggatcag caatcatggc aatcgatgca ttgaaagaac gtggcgcgtc
    18541 gaacattaaa tttgtttgtt tggtagcagc acctgaggga gtaaaagctt tgcaagaagc
    18601 gcatcctgat attgacatct atacagcttc tctagatgaa aaattgaatg aacatggtta
    18661 tatcgttcct ggactagggg atgcgggtga ccgtttgttt ggtacaaaat aagtaaatac
    18721 gaaaaagggt gtgacacaac ttttgtcaca tccttttttg gtcattttta acgtttgatg
    18781 cagaacagct ttttcacagc tttcgtttgc atataaaaaa accatcccaa aaatgcagag
    18841 atggtcaaat tcaaatcttt tttagttctt tgtagttaac gattactaca cccttacttt
    18901 gcgtcatcaa gttcgcattt tctgcagttt catcaatctc aacaacagcg gaatttttca
    18961 attgctttga aatcgttcca gtttgcgact tgccgtgaat tgtgaacatt acgtgagtat
    19021 tgatcttgaa tttttcacca atttccgatg gtacgatagt tgtatcaaat tttccaaaag
    19081 ttgccatttt cataaaacct gcctttcaca tcaattatac cacgttttat gaaaaatatc
    19141 atgaaattaa actaattttc catcggactc tttacaatca gccaaataga gtatataatt
    19201 tcttttggat aaggccagct tagctcagtt ggtagagcaa cgcactcgta acgcgtaggt
    19261 cgtaggttcg atccctatag ctggcattaa aagagaagac agatatatca aggattttta
    19321 atccattgat acatctgtct ttttgtttaa aggaaataaa gtatgctttc ttagcaatag
    19381 agcatgtatt atttctataa aaatttagga gaataaaacg actagcaata tagctgtgaa
    19441 atcaaatatc acatgaactg cccaacttgt aatgatcgag ttgcttttaa tggcagcttg
    19501 gttgaataac aatcttgcca ccccttgtat caaaaggatg tgtgctacag tgtgtaaaat
    19561 atttccatta tcataagtac tgtaatggat aaatccaaag atgattgctg acaagaaact
    19621 agcgacagga atgctgaact tactagaaaa aatagcgaga aaatacactg agaacaattc
    19681 ttctccaagt atcatgatag gcaaaataat aaataataac gggctgaaag cctcatttat
    19741 tggattgcct gttaaacccc atttcaaaat ttgttgcaaa aataccgaga ctgcggcact
    19801 gattactaca ttgataaaaa aatatttggc tatatttttg acaggagcga caggtttcga
    19861 gaacattttt ttcgtatctt ttttaccaaa aacaagtgta aatgctataa atccagtgat
    19921 agttaaagaa aaaatgctaa taatggatgg cgcaaatagc aaccctaaca aaccgagtac
    19981 aacgacaata gtgcccaata tccctttttt aagttctatt ttttttccta aaatgttatt
    20041 catgataata actcctacta ttcagttttt gaagatagag tcagtataca tgaagagtta
    20101 aaataggcaa tgttttttta tggatatttg tattgctaaa aatttatctt atatattcat
    20161 tttgattata ctacttttat ttgtaaaaat tttctagcaa aaccaagtgc ttgcaactga
    20221 ttaagcgtac aattttttag atgttccgag cgtacttcaa tcatttcaaa ggagttgcta
    20281 cttaaatcga tattttttaa atcagtatca aaaaagctag cattttctaa atcattagta
    20341 gaaaacatta ttttagtgag ggaacagtct acaaaatctg cgcttgataa gacagagtgc
    20401 tcaaaggtac tttcttttat gtttgaacca gataaattta catagtccat tttacaattc
    20461 tcgaataacg tgttcccaat ataactttcc atcaatttag tacctgttaa tttacaattg
    20521 acaaatttca cgcgatgcaa catgcttttt gtcaatatac agcctgaaaa atcacagttt
    20581 tcgaatatac agtcggtaat ctccattctt tgaaaatcat tggaagcaaa aaatgcatac
    20641 ttgaaaatag atttgtaaat aattggatgg cagattattt ggttttcgat gacctctttt
    20701 tgtgaaagga tattatacag ttcaaaatct tcgttttcat agatatgaaa aaaggatcct
    20761 ttattcaagc ttgcttcatt aactttagga aaaatcatat ttaattacct ccattttcat
    20821 aaagtcatat taacaaaagc taaaccaact tacattagca gaaaatgaga taggttttcg
    20881 cctttttcga taacgatagt gtcgctatct cttgaaacgt tcttagaatt gccctgagag
    20941 acacaaatta ggcgtaattc taataaaaat cttagaataa aaatttttac tgatttaatt
    21001 tagaatgttt ttaa
//
LOCUS       DACECZ010000037        20957 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.037, whole genome shotgun sequence.
ACCESSION   DACECZ010000037 DACECZ010000000
VERSION     DACECZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.037"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IWO35_001308"
     CDS             591..1280
                     /locus_tag="IWO35_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0316371.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IWO35_001309"
     CDS             1267..2475
                     /locus_tag="IWO35_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0316372.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IWO35_001310"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IWO35_001310"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ0316373.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWO35_001311"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWO35_001311"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ0316374.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="IWO35_001312"
     CDS             complement(6578..7273)
                     /locus_tag="IWO35_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ0316375.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWO35_001313"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWO35_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ0316376.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="IWO35_001314"
     CDS             complement(8472..8663)
                     /locus_tag="IWO35_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ0316377.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="IWO35_001315"
     CDS             complement(8684..9574)
                     /locus_tag="IWO35_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ0316378.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="IWO35_001316"
     CDS             complement(9561..10328)
                     /locus_tag="IWO35_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0316379.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWO35_001317"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWO35_001317"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ0316380.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWO35_001318"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWO35_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ0316381.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWO35_001319"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWO35_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ0316382.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14944..15282
                     /locus_tag="IWO35_001320"
     CDS             14944..15282
                     /locus_tag="IWO35_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316383.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="IWO35_001321"
     CDS             complement(15450..16208)
                     /locus_tag="IWO35_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0316384.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="IWO35_001322"
     CDS             complement(16226..17044)
                     /locus_tag="IWO35_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0316385.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWO35_001323"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWO35_001323"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ0316386.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWO35_001324"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWO35_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ0316387.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="IWO35_001325"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="IWO35_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ0316388.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="IWO35_001326"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="IWO35_001326"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ0316389.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5990 a         4264 c         3838 g         6865 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac ctttttt
//
LOCUS       DACECZ010000038        20181 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.038, whole genome shotgun sequence.
ACCESSION   DACECZ010000038 DACECZ010000000
VERSION     DACECZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.038"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            53..295
                     /locus_tag="IWO35_001327"
     CDS             53..295
                     /locus_tag="IWO35_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0316390.1"
                     /translation="MRKKLKELGIEKQFTLHGLRHTHASILLYQGVNILSVSKRLGHS
                     SLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(358..465)
                     /locus_tag="IWO35_001328"
                     /pseudo
     CDS             complement(358..465)
                     /locus_tag="IWO35_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            complement(954..1718)
                     /locus_tag="IWO35_001329"
     CDS             complement(954..1718)
                     /locus_tag="IWO35_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ0316391.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(1829..2698)
                     /gene="mdcB"
                     /locus_tag="IWO35_001330"
     CDS             complement(1829..2698)
                     /gene="mdcB"
                     /locus_tag="IWO35_001330"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ0316392.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(2723..3799)
                     /locus_tag="IWO35_001331"
     CDS             complement(2723..3799)
                     /locus_tag="IWO35_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ0316393.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(3823..4416)
                     /locus_tag="IWO35_001332"
     CDS             complement(3823..4416)
                     /locus_tag="IWO35_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ0316394.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(4420..6096)
                     /gene="mdcD"
                     /locus_tag="IWO35_001333"
     CDS             complement(4420..6096)
                     /gene="mdcD"
                     /locus_tag="IWO35_001333"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ0316395.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(6086..6385)
                     /locus_tag="IWO35_001334"
     CDS             complement(6086..6385)
                     /locus_tag="IWO35_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ0316396.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(6487..8133)
                     /gene="mdcA"
                     /locus_tag="IWO35_001335"
     CDS             complement(6487..8133)
                     /gene="mdcA"
                     /locus_tag="IWO35_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAQ0316397.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(8140..9051)
                     /locus_tag="IWO35_001336"
     CDS             complement(8140..9051)
                     /locus_tag="IWO35_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0316398.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            9171..10028
                     /locus_tag="IWO35_001337"
     CDS             9171..10028
                     /locus_tag="IWO35_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0316399.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(10215..10577)
                     /locus_tag="IWO35_001338"
     CDS             complement(10215..10577)
                     /locus_tag="IWO35_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316400.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            10923..12362
                     /locus_tag="IWO35_001339"
     CDS             10923..12362
                     /locus_tag="IWO35_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0316401.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(12369..14921)
                     /locus_tag="IWO35_001340"
     CDS             complement(12369..14921)
                     /locus_tag="IWO35_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ0316402.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPKGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYELGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(15035..16366)
                     /locus_tag="IWO35_001341"
     CDS             complement(15035..16366)
                     /locus_tag="IWO35_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0316403.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(16332..17810)
                     /locus_tag="IWO35_001342"
     CDS             complement(16332..17810)
                     /locus_tag="IWO35_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0316404.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(18115..19950)
                     /gene="tdc"
                     /locus_tag="IWO35_001343"
     CDS             complement(18115..19950)
                     /gene="tdc"
                     /locus_tag="IWO35_001343"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0316405.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT         5969 a         4293 c         3456 g         6463 t
ORIGIN      
        1 ttttttcaac ttaaaaaatg gattagtttc atcaaacgcc gtcagcaaat atttgcgcaa
       61 aaaattaaaa gaattaggga ttgaaaagca atttactttg catggactaa ggcatacaca
      121 tgcatctatt ttactttatc aaggagtaaa tatacttagc gtatcgaaac gtttaggaca
      181 tagcagttta gaaactacaa tgtctactta tcttcatatt gttcgagagc ttgaagatca
      241 ggataaagaa aaaatcaata ctgtgttcga tagtttatat aaaaatgata actagtttgg
      301 cacatttttg gcacaaatgc aaaacaaacc cctgtaccac aaaggataca ggggttattt
      361 gtacgttcgc tgactgacat tgaactcact tgctagatgt tttgctttta ctggatattg
      421 ggctgtcatg attctgtgaa gtatttcaat tgttcttgtt ttcatagaaa tgatctcctt
      481 ctgatttcaa gtatatggtt ccgcttacat aataaaaagt agatatttct gccacttgaa
      541 gtggaaagaa agtaagcaaa gcaaacaaaa aataagccat atgctttttc atctcctcaa
      601 cgaaaaagac atacgcctat tattacaaaa attttgccca tctatacaaa aaagaggttg
      661 tgaaaaagct gtcctgcatc aagtaataag aacaactaaa ctaaaatagt gcttcctatt
      721 tttcgtttgg ttgttcttat tatcgcagat gcaagctttt aaacaccatt tagtggagag
      781 gttgtgacag agatgttcag ccttgagcga taagaagcga ttttgaaaaa tcgcttccca
      841 tattttacaa aatcgtgcct tattgccgat aggctatctc tggcacactg tttattcaga
      901 aaaaggagct gcgacaaggc ttttgtcaca actccgttca taaatatttc ctgtcaatcc
      961 agcataatcg gctgaataaa atcggcttgc catctttttt ctttctcaga aaaatcaaaa
     1021 taccatatct gagaaaaata ctgtcgttgt gtcttacttc ctgagatcaa tcgcccatct
     1081 tttcgatcga ctgaataatc acagcatctg aaatcaattc ggatggcaaa actattttcg
     1141 tggatctgtc gataatttcc tacttcctga atagttattt ttttagtatg atttcttaca
     1201 ccgtctgctt tgtttttttg cagaactttc tgatgttcgg caaacagctt gtctgtataa
     1261 cagcttctgg ctttttcagg agtttcttct tcccaagcgg attggatctt catgaatatc
     1321 ttttggattt ccagaaactg tgctttcttt tctttagccg ttcctgacat gcgggacaat
     1381 atctccgctt tcagcctttt cttttgtctt ttattcttga cgatagaaaa agcagaaacc
     1441 cctcctactg acaaaacagc taatgcaaga aatcccgttt gctcataact agaaggacct
     1501 gtacctggac cataataccc ataagaatct ctgccgtaag aacgactgcc aaaataactg
     1561 cccccatatg aacgaccacc aaatgaacct ctgcccgaac ttcgaccgct agatgtacct
     1621 ccaccatgac cacccgcgac agcgaatcct tgggtcggca gagcaaacaa acataggccc
     1681 actaacaaaa tgattgctaa ataattgatt tttttcattg tcttcctcgt tatcttttat
     1741 tacaaaaaac tatttttatg atagccttgc taaaaggaag agtcaataac cagatgcata
     1801 aactgttttt ttagcaattt tgtcttattt agtcattgag taacatcgga caaatgtatg
     1861 tgtcggcagt ccataattcc tctatcttca gcatgaacaa gcttgcagac aagagatctg
     1921 cgcttcctcc aggagagatt tggttttcct gacaaaagtg caacagtgct gtaaatggac
     1981 gatttggtaa agcactttga ctagcagcat gagcaagttt ttgcatttcc actaacgttt
     2041 tctgatcact gcgatgcaat acgcatgtgt catctagcga actcatcaaa actaacaaca
     2101 tatgcatttg tttctgtaca tatgaatcat tatctagctc cttagctgct tggattgcaa
     2161 gcctgatatg cggataccct aatatcgctt cttcatatgc accatttacg ccatattttt
     2221 gcttcaccct atgaccatga gtggtttttt tatgacgata ttttgtatca ggaaaagcag
     2281 caagcttgct gacatcatca agtattttcg ataaagaaaa aggctgttcc atactgattt
     2341 tttgtgcaat gacactgatc aatagcccca ttgcccaaat cgctccttta tgggtattga
     2401 tgccatttgt agcaatcagc attgcctttt ccgcttttcg cccaatctca gcaattcttt
     2461 ctcttaactc ctgatcaata gcatgatgcc atgctatttg agccatttct tcaaaaaaag
     2521 gagttagcgt cttagcagac ttaagaaata aagcatagtc catatcggaa tgtgcacccg
     2581 catccagtgc atccactaat ccaggttttg gacttaatgt tacttctgcg atcaatgctt
     2641 cctctaccaa cgcagcgagt tgctgagtca ctgctttttt tcgttcgtca tttatcatga
     2701 cactcactcc ttcttcctac tattacttgc tgaaaccttt tttgatagct acccaaatga
     2761 tatgaagtaa ccagcttaaa gcaaatgcac aacttgccgt cattagataa gctacaagaa
     2821 tcatgactcc tgaaggtaac tgcagccaac tatctttaaa caataataac agtacttgga
     2881 aaacaaatac attggctaaa tacgtacgat aagcataaac agacaaccat ttgaacaatg
     2941 gcaattttgg cgaatcgttt acaatcattt tggaagcgcc tatgaagaca gcaaagatga
     3001 tgactaaact gtataaactt tggatcgtat tcaagtaagg tgcattcgca aagctcaagt
     3061 ctcctggatg tgcgagcaat gaacggacag aaaagaacgc gataactaag gcaacaggta
     3121 agaaaagata acgtactctg tccaaatatt gatagacttt ttcgtgataa acaaatgcgg
     3181 ctaccccata aatcatgaaa ctagcaacaa accgatctaa taagtaccag ctgtcatgat
     3241 aaggcccagt caatacttgt gtttgataga acagatacca tgcgacatat aaaatcgttg
     3301 aaccaatcaa tactggccat acatattttt tattagcaaa tactttgtaa ccgagccaaa
     3361 caaaaaacgg catgaacaat tggatctgca gcatcataac ggtataccat aaatgaggcg
     3421 ctccatcacc tgtgacatat ttcagtaaaa agcttgcagt atccgtatac ggagtttgtt
     3481 gttggactgc tggaaatagc caaagataag ccgtcgtcca tagaagatac ggaacgacta
     3541 gttcaaaaaa cttttctttc aaaaattccc aataagacaa atgctgactt gtttttacca
     3601 tattataaac aattgcaaaa ataaacattg gtgctgtgta tttaacgatg acgtaaaagg
     3661 taccaagaaa agctgtattt gttgcgctgt ggctgtttgc caaagcaaag ctcaatatcg
     3721 tttgcatcat aacagccgtt acagctgata ctttctggaa gtcacttacg ttgattttga
     3781 tttttttgtt ttcaaacatc tctttctctc tctttttctt tattaccaaa gttctttggt
     3841 taatttaggt ccttcgactg ttttcaatag aatttttttc tctggctctc tcaaatactc
     3901 ttttagtgaa aaaccgcctt gccgagtcac tacttgggta tcgaattgct caaaaaaagc
     3961 aggatgcgat tgaattacct gaattggcaa ttcaatcggt gaatttgcgt acagcagcaa
     4021 atcaaaatca ctggtttctt ttactgccgg aatcccagtc gataattcaa atcctaagct
     4081 gccgccgatt ccccatttac atcctgataa tatgtttcta gcagcatgaa attgctgata
     4141 aacaggataa tccatgattt tctcttgtct atacgattct tgttcgatga tgtcccacgg
     4201 atgaatgact tgtttgacgg catgttttga gatttctgtt gaccatctct gattgcgggc
     4261 aggtccacgt aaaccaatcg gtagtatttc tgcgtgatcc gtcgacgcac gacgaactgt
     4321 tccgaacatc ttttcagtta gaacgaccca ttcaggcaag gtgaaccctt ctatttcatg
     4381 tttatcaaaa aggacaatat catgtgcttg aatcattttt tatgcatccc attcttcgtc
     4441 cattttggca cggactttat ttgtttcgac acgtccgaat gttttggctg tttcgtttgt
     4501 gtatcgattt gtcagcatcg tgtcatttgt attgcggaca tcttgaatgg cttcattgat
     4561 tgcttcgatg actttgctga cattttgttc tgaagcagct tggtcggtga tatcttctag
     4621 gaaatgatag atcgctccta gttttgtata attattgata tcgtaagcga tagctggaac
     4681 tttttcagca gctgcttcga tttcagcaac cgaacgtttt gtcacacgtg cagcactttc
     4741 tttagacatc gcttggacgg taatttcttt attgtttaaa gcaatcaagc ggcttgattg
     4801 taatccatgt gctaaaaatg cacctgaaat agcatttcct gcaatcaatc caataactgg
     4861 atgtccagct tgacggagtt ttgcataagc agcagcacta ttagccaaag ccacatggat
     4921 accgatcaat tcttcgttat agccgtatgc ttggcttggt acatcgatca caacgatgat
     4981 tggccgtttc acttttttgt ctttatcttc ttcgtaaacg tgatccaaaa tttgtcccat
     5041 gcggaaacct tcaactaaac caacttcacc gtttcgtaca cggtacatcg ggtttttagg
     5101 atcaggtacg attgatgtca cgatggcttc ttctatgaat ttacttgatt taccataata
     5161 cacagtacta atagaagaag ttggattttt catgccagtt agtgccgcaa accaatcgta
     5221 accaacactt gctgcagctg gttcatttcc ttctgctact tctggtaagt caattgattt
     5281 tgcttgatgg ttagcaattg cttcattgta atcgtcaatg gccattggtt ttgaaagatc
     5341 taaagaatca agtaatgata aataaaatgc tgagctttca ctgcgatgcg tgtcttttcg
     5401 ttcgtccatt gctgcgatga cagcagcttt gattgcttct acactatcag tcactaattc
     5461 atcaatgatt tttgattcta cccgttgacg agatcctaat gtattccaaa tcaagtcttt
     5521 gtcactagaa tcaaattctc gaacgcctgc ttcttgttca ataacttctg gtccattcaa
     5581 accgactctc gctttttttg ttgcgattaa ataactcatt aatgctgaag taatcgacat
     5641 accgccaaat gatcctacac gtcctggaat caaaccgatg actggtacat attgttttag
     5701 agcaatgatt acatttccga tttctgaaat agataacagt ccgtagtttg cttcttgcaa
     5761 acgaacacca cctgtatcta gtacaatgac tggaaagatc tcatttcctt ttttattttc
     5821 ttctaacaca tgttcaagtg cagcaacgat ttttgcaccg gaaacttcac cgataccgcc
     5881 accttggaat tttccttcca tagcgattac tactgcttgt ttgccattga tcgtgccacg
     5941 gctgacaacg atcccgtcat cactttcagg gacgattcct tgtgctggta aacttggcgc
     6001 gatgatttga tcgaatggat caatcaattc acgtccattg tcaagtagag cgttcactcg
     6061 ttctctggcg tttaattcta caaaactatt tttcatctga aagttcctcc attgcttgcg
     6121 tcaatcgtag gtacacaact cctggtgtag caccgaagtc attgatcacg atatctgctg
     6181 aaattggata acgatcaaaa aatctcgtta atacattgtt ccatacttcg ccgaagccat
     6241 cgcttcctgt acaaattttt acagtagatt ctgttttttc tgttggaaac atcaagattt
     6301 ccaaatctcc tgaaccgacc actcctacat ggattttctt attgatcggc tgtttcgttg
     6361 gatatttata agctaattgt tccatggatc tctccccttt ttgtaaaaca gagcatacac
     6421 gttctgctta ttcgtctttc ttcttccatc tgggtaaatg gcgacaaaag gtttttctcg
     6481 ccaattttat gaccaagttc tgaatttcat tggtggttca tacaggccat ctgaccattc
     6541 gatcaaatca tcgatggttt gtgcagctaa taatgaacgt ttcgcttcac tacgtttgat
     6601 gcctagatct tctggtaatt tcacgatgcc ggctgcacgc aagctatcta gtgattcttt
     6661 cgtgctagtc attccaagag gtgtaacacc gccaattgct gcaattgctg cttgtcgttc
     6721 ttcttttgtt tttgctttgt acaaataagc aattccttct tctgtcacga tatgtgtagt
     6781 atcttcgcta taaatcatca ctggtgcatt ggctaagcct gcttgttttt gaacagctag
     6841 tgcatctaat tcttcgacaa agactggttt tttatttgaa ccatatgttt caaccatttg
     6901 aacaactaat ttttggcctt tccctaatgg atcatcattg tcgcgaagtg attgccatgc
     6961 tggtgtagaa tgacgacgac cgccaggatt atgtcccatg ttaggcgcac cgccaaaacc
     7021 agataaacgt ccttttgtta cggtagaaga gtttccttcg taatctaatt gcaaggttga
     7081 accgataaac atatcgatgg catattgtcc tgcagcttgt gataatgttc ggttactgcg
     7141 catcgtgccg tctttaccga caaaaaagac atctgggcga gcagcaatat atttttccat
     7201 accaacttcg ccaccaaaac tatggatgct ttcaacccaa cctgattcga tggctggaat
     7261 catcgttgga tgtgggttca atacccagtt cttcgcgatt ttccctttta accctagtga
     7321 ttcaccataa gttggcagta acaattcgat agctgctgta ttgaatccaa taccgtgatt
     7381 caaagattgt acgccgtgtt ctgcgtagat tcctttgatt gccatcattg ccattagaat
     7441 ttgcaattct gtgatgtttt gaggatctcg tgtgaataaa gcttctaatt gataaggttt
     7501 atctgctttc acgacaacgt ctacccagtc accagggata tctacacgtg gtaaagaatc
     7561 gacaacttcg ttgacttgaa cgatcacgat accatctttg aaagctgcag cttcgatgat
     7621 tgttggtgtt tcttccgtat tgaaacctgt gtacaagttg ccgtctttat ctgctttatc
     7681 tgcagctact aaaacaacat ttggaatcaa atcgatgaat aagcgtccat aaagttccaa
     7741 ataagtatgg atatcaccta atttcattgt gccgtcttca atcatttggg cgacacgcaa
     7801 gctttgtgca ccagcataag agaaatcgat cttgcttgcg attccttttt caaaaatatt
     7861 caaatgttct ggtcgtgaga tactagacat gatcaaatgc agatcattca ctttttctgc
     7921 atctactttt tcaagacttt ctgaaagaaa acttgcttgt ttttgattgt cgccttctaa
     7981 tacaactttg tcgccgggag caatcagttt ttccattact tctacgattt tttcagtttc
     8041 tacatacttg ccattgagca agttgctgat tttttccaat cgctgcgctt ttttttcgcg
     8101 tttagaagcc cagttgcggt ttggtttttc cataattagt catctccatt tttgatatag
     8161 aaacgtggcg tcatcaatac catgttggtc tagattgatc gtacggatcc ctttatctac
     8221 accaaatttt gtatgaatca gctttgtcaa agtattgccg gggggaaatt caatcgtcag
     8281 atccattcct aattcttttg ccaagtccat catttggttc cactgcacag ggaaagctag
     8341 gttttccaac aaatctttac gaatctcttc tgcgtcctga gtggctcttc catccgtatt
     8401 ttttatatac aaacatctag catcagatag atggacagac tgcatatacg gcttgaattt
     8461 cttgaccgta tcattcatca agagacagtg agatggcaca gggactttca gcagcttcgc
     8521 tgtttgcgct tggtgccgtt tggcttctgc caacgtaacg gataaacctt cgatactccc
     8581 agataacgta tgttgcatcg gacaattttc atttgacaga taaactggat ggtcaggatc
     8641 aaatgttttt gctacaattt cctcagcttc gcttctcgtc acacctacta ctacgcccat
     8701 tgcataaccg cttggataag cttctttcat taagactgcg cggtgataga ctagttccgc
     8761 tgcattctca aatctcaaag atcgacaagc aacagctgca caaaaagcac caatcgaatg
     8821 tcctgctgca atatcaggct gaataccgtt tttttccatt tcatctaaat aaaatactgc
     8881 ttttgtcagt aatgctagct ggataaaaac cgtatcttga tagttttctt ctttatcaac
     8941 taaccgatac cccgtcacat tttctaaacg ttccatgtac atttcaggga tatccgctaa
     9001 catccctaac ttctgactgc cttgtccagg aaataagaaa cctgtactca tgagcctcgc
     9061 ctcctcagca cttacttttt ccactgctat cttagtcaat ttctgctata ataaaaaatg
     9121 ccaaaaaaag aaaaaaccat aacaaaaagg tatggatggg atggataatt atgaaattgc
     9181 ttcatctgca gtattttaaa aaagtattcg aaacaaaaaa tgttacccaa gcagccaaag
     9241 aattatttat ttctcaacct gccttgagcc gtgcgatcaa acatttggaa aacgaattag
     9301 gtgtcccttt gttttaccat agcggcagaa acattgagac gaccgtttat gcagaagaat
     9361 tttatccata tgcagtcaag acattagaca cattagaaga aggaatcaaa ttattgtctt
     9421 cggtcaatga gaagatcgtt acttccgtca tattgtattt agaggtagcc tctgtttcta
     9481 ttccgaactt ggtccgaatt tttatggaaa agcatcctga tatccaatta acgatcatgc
     9541 aacatcattt accagatgat attaccgatc ctgtactgta catcacttcg gagatgaagc
     9601 ctggcatgac caatattcct atcatgaaag aacctgttta tgtcgccatt cctaagaaac
     9661 atcctcttgc taaaaaagaa catctgcaat tagaagatat ccaacgcacc cccatcttga
     9721 tgctgtcaaa gagcaacgcc ttccgtcata cactggatgc agcacttgaa gcgaaaagta
     9781 tcgaattgac gatcagttcg acgacagatg atcctgcgac attacgttct attgtcaagc
     9841 aaggattagc tgttagcttt ttcccaaaag tttcttggtc ttacgataaa aatgatcctt
     9901 tcgtcttgcg ggaaatcaaa gatttgccct tgacacgaac aatttattta tcttctagct
     9961 tcacagaaga tcatcctttg atcaaaatga ttgcaaaaac gatgcgggaa ttttatttag
    10021 gaaaataaaa aaaaaagagc tgggacaaaa ttcttgtcgc agctcttttt tcgaacaaac
    10081 ggtgttccag aagtaatttc ttagaaaata agccgaaatt tcccaaaaat ttgagaagca
    10141 attttcgtaa attccttctt atttcttgaa gctaaacatt tctgtcacaa cctcttctct
    10201 attgtcctag acattcattg cgttgattta aaacttccat caatcaaatc tgctagttca
    10261 tctagctttt ctattttacg tagatcaata gagatccaat gttctttatt catgtgatag
    10321 gctggaaaat acccctcttt tcctcgcaaa attccgccaa gttcaggatc gaccttcaaa
    10381 tcaagaatat tgatgatcct atggtcactt gcatcatcat agattttttc ttctggaaca
    10441 gacatcagga ccccaaacca ttttttgctt ttttgatgtc tgaatacgca ataatcagga
    10501 aattttttaa aaatatattc tggagacaaa ttgtatgttt cattgatgtg atcgattagt
    10561 tcttttttcg ttagcatctg cttccatctc tttcttattg attcgcttta ttcaaagata
    10621 ccacgtaaga atagaaaaat gaatacaagt agttgctaat caaatacttc gatatttttc
    10681 ttttttatca actcttaaat gttcaatccg cctgtgttgg tcttgctacc atctactatt
    10741 aaaatgttta ttattgtttg cccacctatt ttttgatttt agtggtatca tttttcaagt
    10801 tcatgatgaa attcttatca tcagtaaatg ctccagaaaa ataaacaatg attgaaaaat
    10861 cagttgaaac ttgaccaccg tttattttct ccgtattctt agaatagaag ggagtctgat
    10921 tgatgtattc actaatgaaa aaaatcatta ccgaaaaaga tattcgacgt catgtttctt
    10981 tagtagaaca attgctgaat catacaaaga taaccgtaaa tgaattagct gaaatcatag
    11041 ggacaaccga acgaacgatt ttttctgacc ttcaatctat tcgctctcag ctgcccgaag
    11101 gctgggacat catcagcgat caagcaggaa ttagtttgca aaaccagcaa aatttattaa
    11161 caaatgacct ttgggagatt tttttcaaac aatcagtcag tgtcgaactg ctgaaaaatc
    11221 tgctttttac aaaaaaagtt gctgtccctg attttctagc cgaccatggg ctttcttatg
    11281 ggacattgaa aagacatgtg actaaaatca atcagcgttt agcaagttac gacttgcaga
    11341 tcgatttgac gaaatataca gcttgtattc ttggaaaaga gcgtgcgatt cgtacttttt
    11401 atcatcgatt gctgattcct ttcactcata acaactattt tttcgaggat tattcgatcc
    11461 atgaatccca ttattttcaa tttttaagaa acctcagtca aactgaacta gcggttgaaa
    11521 cagaagagat tttcggaaca tgttggttct ttatcaatac gatccgtata aaagcgaatt
    11581 gccgattaga ttcttctatc cataccaaca gcacactctc ctccctgtat gactctgcat
    11641 tgaaaaaatt gtatttaacg gaaggtattt atcttaaaga cactgagctt tcttttgctt
    11701 ctttctgctt tctggaaagt tggaattaca acaataacta tggacaagaa accgctcgct
    11761 gcctgcatca ttctccattt ttagaggtcc tcgaaacgtt tgtagaggaa ctagcttcag
    11821 agctgtctct tgatcaacta aaaaaaacat ctctgactga taacttggct ctcctcatat
    11881 taaaatatca tgaatcgccg atattgattg aagcattgga ccgtcaatac catcgttttt
    11941 tagaaacata tgatcagcaa tatgcacact tgtatcaaca gaaagagaat ttactgaacg
    12001 atctaaaaaa agaaatagaa atcaatgaac caaattactt tctccaactc ttgtctctac
    12061 tgatccaaca aacgattttt tctatcaaac cgaatttgtt caatgtctat tttattttcc
    12121 aaggagaacc ttcatggaag gcgttcttgc agcaggaatt aaaagattac ttaggaaaac
    12181 gtgtctgctt cttacctatc gaactaaccg atctttccac tatttctttt gaaaaaacag
    12241 atatcttgat ttccaatttc cctctagatt ctctagatat ccctattgta tacatttctt
    12301 ctattccaac gaaaaatgag ctcaatcgtt tgactgaact cactttcaaa acttttttat
    12361 agtaacggct agcgactgta ttctctagct gttttttctt ttttgcgaaa cagtttgttc
    12421 atgatccctt tttctatttc tacaactaag aatagaagaa tcacatttgc tccaatcaat
    12481 aactgttgtg tcatcgttaa acttactgta ccgatcagct gctggccgac tggtagaaaa
    12541 ataaccatcc cttgcaataa agcaaggata cctaatgaaa gaaaaagcgc tttgttttgc
    12601 aaaagacctt ggttcaatga cggcttggac aactctctgc aattgatcat gtaaacagct
    12661 tgagctaaga cgatattttg cagaagaatg gtttgctgta agctttgccc ttcaaactgc
    12721 atagccaata aataggcagg aaccatgatc agtagggaga catagatgat ccgaaacaaa
    12781 tgataaccag ataggattcc ttgctttgga tctcttggtg ggcgcttcat gatttctgga
    12841 tctggcgact cgaaccctag ttcataagat aaagtgattg tcgttaccat attgacccat
    12901 aaaatctgta ctggggacaa aggtagcggg cgattcagca taagtgccca aatcaagatc
    12961 aatccttgag ctaatgcagt tggcaagaag aaattgatgg ttttttgcaa attatccaaa
    13021 atccgccggc cctcttttac ggcttttgca atcgtatgga aattatcatc tcctaaaacc
    13081 atatcagctg cctgtttgct tacttcacta cctttgattc ccatagcaat tcctacatcg
    13141 gatcttttca aagcaggtgc atcatttacg ccgtctccgg tcattccgac gatttctcca
    13201 tttttctgca aagctttaac aattcgcagt ttatgttctg gtgtcgttcg agcaaagaca
    13261 tcgactattg gcacttgctg aatcaactct tcatcagaca taagatccaa atcgatccct
    13321 tctaaaactt tggcagtatg ttttaagcca atttgttccg caatcgcttt tgctgtgtct
    13381 ttgtgatccc ctgtaatcat tttcactgag atccctgctt gttggcattc ctttacagct
    13441 ttgactgcac tttcttttgg cggatcgatc aatccggcga tccctgcaaa agttaattgg
    13501 gttaaagtct catgcgtgag ttcctgtttc gaatctactt ttttataagc aaatcctaaa
    13561 acacgctgac cttttctcgc aaattctgca gctgtctgac tccatgcttc ttgttctgca
    13621 gaagaaagag ttgatttagc aaataatact tctggtgcac ctttgacgta aatcaccgct
    13681 cctttttcct ccgcatgcat cgttgccata tatttgtatt cagaactgaa cggaatcttt
    13741 gcttctattt ttttcaaagg tagattggct gtttctgtgt gtcgaatcag cgcaacttct
    13801 gtcggattgc cacttaaatt ttctgttttc tgttcttctt ttgtttttaa ttcctgacag
    13861 tttttcataa tggacaacac gcaggcttct tcttttgcag ctacatccac aactgtcatt
    13921 tcattttttg tcaatgttcc tgtcttatct gaacagatca ctgtcataga acctaatgtt
    13981 tctaccgaag gaagtccttt gatgatcgcc ttttcttttg ccatttcatg tactccaacg
    14041 gatagaatca tggtcaaaac agctggcaat cctttgggta ccattgacac caccaaagca
    14101 atcgtagccg aaagaaggat atgccaatcc ataccatatc gaaatgtcgt aaaaataact
    14161 aagaataggc tcaaacaaag aatgccttga aaaatttgtt tgtttaatcg gttgattttt
    14221 ttaatcagag gcgtcgtttg ttgttcgacc gattgcaatg cttgattgat tttaccgatt
    14281 tctgtcgtgt ttcctgtttc tacaacgaca ccgactgcag aaccagattg tacaagcgtt
    14341 cctgaaaaag ccatattttt ctggtcgccg atcgattctt cttctgtcaa aacggcatga
    14401 cttttttcag atgcttctga ttctcccgtc aaaatcgatt cttccactaa taaatcatgt
    14461 gcttcgatta atcggatatc tgctggaacg acatctccgg cacgtaagac aatgacatct
    14521 ccttttacca gttctttcgc agggattttt tcttgcatcc cgtttgatag tactatcgct
    14581 tcttgtccca tcagctgctt caaaccattc aatgattctt ccgcttttct ttcttgccag
    14641 taactaacga gtccattgac gatcactacg aataaaataa tacttccttc aatatattca
    14701 ctagaagcaa atttcaaaaa agaggcaatg atcaacacga tcatcagcag atcagtaaaa
    14761 tgctttgcta tttttttcca tcgttttgtc tgctgccttg cttttatttc attatatcct
    14821 tgatcagtca ggcgtttctg tcgttcttct tgcgacaagc cttctgttga tgtttgatag
    14881 tttttcaata catcttctac cgtcaatctg taccagttca tcctatcccc tcctgtttaa
    14941 ttgatactgt tattatacgc agggaagttt tttcaggcgt atcgattatt tcaggggggt
    15001 ctgaaataat tcgttttgca aaatgtatct gggcctaact gatatttcta ttgcttattc
    15061 ttttatatac aaaatttaag aatacaaacc agataaccaa taaaaaagga ataaagcgtg
    15121 tcgtgctttc gaataacaag ctgaaagcaa ttgaagtaaa aaacaccaac aaagcaagat
    15181 ctttatatct ttgtgtagtg ttccgtttct tttctttaat aaattgaaca tgtgcacaca
    15241 agatcgaaga ccacacaaat agaaaacaac tcgttgtcat gcctgccaaa agctgaaaaa
    15301 acaatcgact atcagctaca aaaaaatgta gacaaagacc ggcaaaaaat aacattcctg
    15361 taattaaaat accgtgtaaa ggtacgcctt tcttagaaag cgcacaaaac caatggggca
    15421 aacttttttc ttttgctgtt tgatataaaa tcctgctagt actgtagact gcactatttg
    15481 ctgaggacaa agaagcaacc aaaatcatta gattgacaat tgctgctccg ccttggcaat
    15541 ggatcacttg cagcatagtc actaaaggac tttcttttaa atctaaacaa tgccatggaa
    15601 caacaaataa aatcgacaaa atcgttccta tatagaaaaa gagaatacgc caaggcagac
    15661 agttgattgc ttttttcaaa ttcttttgcg gatcacttgt ctcgccaacg gttaagccga
    15721 tcatttcaat tccagcaaaa ctgaataaaa ccataggaaa tgagactacc attccagcaa
    15781 agccaaacgg tagtaattca tcgatctgtc caaaattttc tttgattccg atagactggg
    15841 tatgaaaatc tgagacactc aatcctatac ccaataagat aaaaaggaaa atccccaaga
    15901 tcttgatgat tgagaaaaaa gattccaatt caccaaacca cttgactgca acaaggttga
    15961 ctgccattaa gatcattagt gcgctacctc cgcttatcaa agctggtaca tgtacaaacc
    16021 agaaatggat atacaatcct attgctgtta attccgcaag tccagctcca agccagcaaa
    16081 gccaataagt ccaattgaca aaaaatgccc actttttgcc taaataatag tcaacgaaat
    16141 cactgataga gttaaatgta ctatttgcta aaaataaatc acccatggcc cgcatcaaac
    16201 cataaatagc caaaccgcat atcaaataaa cgagaataac agctggccct gctgatgcaa
    16261 ttgcctttgc tgaacctaaa aataatcctg ttccaattgc gccacctaat gcgattaact
    16321 ggatatttcg attagttaga gtccgtttca ttgccggttt ttccatcaag ttcacctttt
    16381 tctttttcaa gcagcatggc gacttcctgt tcccaagaag gtttctttat tttttcaata
    16441 cagagtggta ctacaaacaa aatgacactg atggctgtga ctgcaattag attgatccat
    16501 gttccagctt tcatcgtcag tccgacagaa aatccgattg ccgcaaataa aacaataacg
    16561 acagtccaaa ctccaagatt gctctttttc tctttttttc caattctaaa tggtcgattc
    16621 aactttgcat gtgtataacg caatctcaaa actgcgatcc ccatgatgac atagacgata
    16681 cagtagataa cagtagttga atttacgagc atcaaaaatg cttgattgac gccaggaatc
    16741 agcaaataaa aaagtgcaaa gattgagata atgacagact gagtcaaaat aacggatttg
    16801 gatacgtcaa attgattcgt cttccaaaaa ttgaattttg gtggatacgc accttttcta
    16861 gcactttctg tcactgtctt tgctggtcct gaggcccaag cagataattg gactgctaca
    16921 ccaatgaaga caagtaaact aaacagattc acaataagat gtggcaaacc aagaatttgg
    16981 caataaatcg aaatagattg cgcgatatta ttcaattcca tttgtccttt tggtacgaca
    17041 tttgctacaa ctaaagcatt caaaatattg acgaaaaata taaaaattaa cgcggcaaat
    17101 actccgcgaa tatacgtttt aactggattt tccaatcgag tgatatacac agaggacatt
    17161 tcgataccag taaaaataaa catgatcggc gcaaaataaa caaatgagga tgcagtcgta
    17221 gtatctggta ttaatttgtc ccatgaaaaa ctgcctaatg ttccggttgg tacgatccca
    17281 actttgatcc acgcagcaaa ccctaataag agcatcatcg tcaaaggcac gtacaatcct
    17341 aaccaaacac cgacttttcc gccgattttt gccatatcaa atttcaaatt caaaaaagtg
    17401 atgatccaat aaacgactaa aatgacaact aatgtaaatt tgctgttcaa accaagcggc
    17461 acattatcga taatattacc aaacaacgga gcgagtgctg aagcgaccat caccatccca
    17521 ggaaacattt ggacccataa cagccatgag acaacgaacc cccatttttt tcctagagca
    17581 ttcgataccc aaagttctgg accacctttt tggggaaaca ttccagcaaa ttcaccagag
    17641 atcaatgaga ttggaaaagc gtacaataat actgcaacca gcatgtaaaa aatcatcgtc
    17701 cagctcgttg ctgctacatc tggaatattt cgtacacttg ctacaagggc gcaggtcatt
    17761 ccgataaagg tcatggtcgt gatttgcttt gatgaaccag tatctttcat ctttattcct
    17821 tctttcaata gcgacatatg tttttgtttt cttttatttt tgttcaagaa cagaaatgac
    17881 aaggtttcat cgattcagta caaaacccag tatcaccgaa acatccattg aacgatctta
    17941 gtgaactggg tttgatacat gaaacgaacg atccaactta tagaacgttt tataaaaaag
    18001 aatatcttgg cgaggaatat atgaaaaaca gggggaaata caattgtttt gatgaagcaa
    18061 ataattgtct gttctccata tgtctcttgt tattctttca cacaaaaatc agttctattg
    18121 gatgatttcg tttagtttct tttccattgc tttggcgatt ttttttgtat aaaaattgaa
    18181 aattcgatca tcattcaaat aaggagtcat gatagccgca cgtagcagag tgattttctg
    18241 ttcttttttc cattcttctt tcgttagacc cattctttca acaaaacgaa tcggagaatc
    18301 accgtaatct tcttgtgtaa atgtagtatg agaagtaata aaccgttcac cataaacatc
    18361 gccatccata taagaagacc gatcgaacat tttttcattt aattcattga tagcgttcaa
    18421 atctgtacag ccttgttctt tgaataccca attgaccata ttaaaatcag gatgattcaa
    18481 tggatagact tcgattgttt ttcctttaac agtaaaagtc agatgatcga gaaattctct
    18541 gaatctttga gctgcctcga ttgaagcccc aatcaattga ccatatccgg tcacattcaa
    18601 tggcaataca cgatgtgctg tccagactgc ggcggcggtt gctccagctt ttgatccttc
    18661 caaaatataa gctcctaaca tatctggagc ctttactgat ttttcaaaca catatggcgc
    18721 gaaataagaa atgatatttc tcatattttt atgtttgatt acgattcctc cagcagcata
    18781 agggacatag cccatcttat gaggatctat cgtgacagaa tccgcttctg aaatagctct
    18841 gaacccttca tatacctctt tgtcaatcgt tacgcagtgg tggaaaacat gatgttcttc
    18901 aaagaattca gctaaggaag catacggcac aaattctcct gcttcgttta gaaacagtga
    18961 acgcgcatag ccgccatatg cggcatcaac atgtaaatag aaatagatcc cttcatcttt
    19021 taacctttct cttaattgga cgattttatc tacactgtct acttgacctt cttctgtcgt
    19081 tcctacgaca gcaaccacac ctagaattgg gattttttgt ccagctaatt cacgaattgt
    19141 tttttccaaa gcattaatat ccattcgata atcctcttga actgggatag caaccatctg
    19201 atctaagccg actccgcaaa tatctaacgc cttcatccaa gaatagtgtt ttgtttgagg
    19261 gaccagccat ttccctaatc tttggatatt ttttccgcta cgggaagaag ctgctttgac
    19321 ctcatccatt tcttcgtccg taaagctttc ggtcatttcc aaaatttctt caacagataa
    19381 attcaaaagt tcccactcag acattttttt cactttttca ggcagcactt ccttgacagc
    19441 taaaggaatc gatttgatgc aacgggcgta ccataatcct tcaagattag caatagaacc
    19501 atctgctgca atgtgtcccc atccgtctgt catattgaat aatgaagcaa aatcctgtcc
    19561 tacttctgct tccatttgcg aagttgccat agaagattcc aatgcaacat tatttgggtt
    19621 ccacagcatc gcatagttgt aagctaataa agcaggcatc aatgtttcag cattcatttg
    19681 tccccagtaa cggcctgctg aatgccatgg gatcgatcct gcgcgtaatc gctgactgac
    19741 ttcatccaag acctctatca tatggtcgcg ggtggctaga taatctggag tgagcttatc
    19801 tccttcgcta attgcaggca tatctgaagg aatataattt tctctccatc caagatgttc
    19861 atctaccatc ttgttcaaca acattttata aactggtcca ttttcagctt tgtcgcctat
    19921 aaagacggcc ttgatatcca tatctttcat ttttcttccc tccatatttt gttggtcaat
    19981 gtcttgcaga tacatgtgtt tatctgtaac acctcttcac tatagcgcca aaaatgagaa
    20041 attttttttt ataacatcaa atccacgtag atttttacaa acaaaaataa ataaagtcgg
    20101 atttttctga taattcaacg caaaaaagcg aacaattttt caataaatca gtcttctaaa
    20161 atagataacc taaaaaaagc g
//
LOCUS       DACECZ010000039        19849 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.039, whole genome shotgun sequence.
ACCESSION   DACECZ010000039 DACECZ010000000
VERSION     DACECZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.039"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(26..178)
                     /locus_tag="IWO35_001344"
     CDS             complement(26..178)
                     /locus_tag="IWO35_001344"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316406.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(369..1247)
                     /locus_tag="IWO35_001345"
     CDS             complement(369..1247)
                     /locus_tag="IWO35_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0316407.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1286..2404)
                     /locus_tag="IWO35_001346"
     CDS             complement(1286..2404)
                     /locus_tag="IWO35_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ0316408.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2401..4500)
                     /locus_tag="IWO35_001347"
     CDS             complement(2401..4500)
                     /locus_tag="IWO35_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ0316409.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4551..5372)
                     /locus_tag="IWO35_001348"
     CDS             complement(4551..5372)
                     /locus_tag="IWO35_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0316410.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5369..6178)
                     /locus_tag="IWO35_001349"
     CDS             complement(5369..6178)
                     /locus_tag="IWO35_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0316411.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6199..6693)
                     /locus_tag="IWO35_001350"
     CDS             complement(6199..6693)
                     /locus_tag="IWO35_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0316412.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6705..7193)
                     /locus_tag="IWO35_001351"
     CDS             complement(6705..7193)
                     /locus_tag="IWO35_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0316413.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7270..9966)
                     /locus_tag="IWO35_001352"
     CDS             complement(7270..9966)
                     /locus_tag="IWO35_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0316414.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(10394..11467)
                     /locus_tag="IWO35_001353"
     CDS             complement(10394..11467)
                     /locus_tag="IWO35_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0316415.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(11464..12291)
                     /locus_tag="IWO35_001354"
     CDS             complement(11464..12291)
                     /locus_tag="IWO35_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0316416.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(12292..13098)
                     /locus_tag="IWO35_001355"
     CDS             complement(12292..13098)
                     /locus_tag="IWO35_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0316417.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(13110..14195)
                     /locus_tag="IWO35_001356"
     CDS             complement(13110..14195)
                     /locus_tag="IWO35_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0316418.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(14212..14754)
                     /locus_tag="IWO35_001357"
     CDS             complement(14212..14754)
                     /locus_tag="IWO35_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0316419.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            15012..16001
                     /locus_tag="IWO35_001358"
     CDS             15012..16001
                     /locus_tag="IWO35_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ0316420.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            15998..16735
                     /locus_tag="IWO35_001359"
     CDS             15998..16735
                     /locus_tag="IWO35_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ0316421.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            16732..17283
                     /gene="coaC"
                     /locus_tag="IWO35_001360"
     CDS             16732..17283
                     /gene="coaC"
                     /locus_tag="IWO35_001360"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ0316422.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            17299..17931
                     /locus_tag="IWO35_001361"
     CDS             17299..17931
                     /locus_tag="IWO35_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0316423.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            18016..18663
                     /locus_tag="IWO35_001362"
     CDS             18016..18663
                     /locus_tag="IWO35_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0316424.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            18668..19651
                     /locus_tag="IWO35_001363"
     CDS             18668..19651
                     /locus_tag="IWO35_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ0316425.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         6079 a         4262 c         3462 g         6046 t
ORIGIN      
        1 tattttatac ggctgaaaaa atctatcacc aaaaaaattc aaacattaag ccaaggatta
       61 tgaagtcaac atttcgaaaa atgaacatac attggtaagc aactataact attaccacag
      121 acataaagta acacaatata ttactattta agtgtacgcc accagtgaat atcctcaagg
      181 tatacagcgc acaacaaaaa gaaaaaagga aacgcagaag catttccttt cagtgattta
      241 atgaaaacaa cacgatttga cttagagcct ttttatttga attcattttc gctttatcca
      301 acttcatttg atcatttgat tactcggtta aatacttttg acatgagtat caatgaaaca
      361 ttcattaatt atggttgctt atttcttacc gcttttctgg ccaatgctaa tccgccgatt
      421 attccggcat tatcacctaa ttcgggcgta ataatgtagc tatcaattgg tggtatttca
      481 acatatccat ttataagctc aacaaattta tcctgaactt ttttcttcat gtgttcttgt
      541 ttcattacac caccgccaaa aataattcta tctggcgaaa acatcagtgt tgtattgtat
      601 gcacattggg caaggtaaaa ggcttcaatc tgccaaaacg aatcgtcagc caataaattc
      661 tggcctttta cacccaaccg tttttcaatc gctggtcctg ctgccatccc ctctaaacaa
      721 tcttggtgga agggacagtt tcctgaaaaa ttgtcctttg gatgtcgctt gatcaacata
      781 tgccccattt ccggatgact gaacccttcc acgaaagtcc cattgaacaa ggcaccacct
      841 ccgatacctg ttcccacagt gaagtatgca acacctgcta accctttgcc agaaccagca
      901 acatattcac catagcaagc cgcattgaca tctgttgtcc attcaatcgg aatattgaaa
      961 tattgcttta gcgttcctac aaaattgaaa ttttgccaag caagttttgg tgttgatgtg
     1021 atatgaccga aactcgttga tttgcattga atatcaattg gaccaaagca gccgaccccg
     1081 atgcctgcca attgttcctt aaaaggttta aaaaaatcaa tgacctgctt cattgtttca
     1141 actggagttg ttgtgggaaa actgactctt tccaaaatcg ttaattcctc agtaccgact
     1201 gcacagacaa atttagtccc tccagcttca attgccccat atatcatcat aggttcctcc
     1261 cttcgaatat gtgaaaaaca cttcattaac gcggcaagcg ttctcgatca gtgaaatgac
     1321 gtttagtatt tcctgaagct tctgttgaat accagtaagc gacactagca acatcgtctt
     1381 gccgctcaaa taagcgaaca tcatcgttgc cgatttgttg taaagtgacc gataaatctt
     1441 cggtaaaaca gatggggtct tgaagatgcc aacgataaag accatgtcgt ggcattgcgt
     1501 cgtcaccaaa ggcatgaacg gcattcaatt ttcccgtttc aaaacgatct cttgtggcat
     1561 ctctatttga ttggtaagga taccctaaaa aggctgttga atagtttttc gcatgtggac
     1621 gtccgaactc atcgtactca tgaaaggccc acgccccgcc aaaataatct tctgaacctg
     1681 ttgaggttac ggtcggatac tcactatccc catcaatgta gaatttaaac tcgccttcgc
     1741 cccaccaata acgttctagg caggtaagcg caaggtaagt acccacgtaa tagccttgac
     1801 cctttaatcg atcaattaac ttataatcct cctgtaattt ggtaatgcgt tcccgatgcc
     1861 aatttgcgtg aaaatatagg ctattttctt ccaattcgtc caccaaagcg taatttatcg
     1921 tataaaaaaa tgaggacata tccttcggat gtaaattctc gatcgtaatt ttagcggatg
     1981 tttggaaagg catttcgaaa taggaattaa agccccctgt aggattaacc actatcggca
     2041 acgagttcac ctcgcaacgc tctccaaacc cattacaaaa gaaatcccct aaaggacttt
     2101 caacagcagg gctatccgac ccatcccaat aaattcttaa gattaaatca cgtaaaacaa
     2161 aactgccaaa ttctgttcga tcacgaatcg tcatccacat atgacgaatt attcccgttc
     2221 cagtaatatc cgcaatcaca gtttcttcac cagaagtcag cggaattgcg ccacgacctt
     2281 tgcggcttgg tcctagctcg cttggcaaca tcgcagcttt acctttctcg cctgttggat
     2341 tttcgggagt gattgctcga ctctggattt ttttaaattg ggtaatattt tttagtatac
     2401 tcatcttttt ctcctttgaa tgtgaatcgt ttttcccttt tctacgtgaa gaattttttt
     2461 cgtatcactt attttcaatg gcttttctga caaatttgaa atactcaact cggattgcgt
     2521 acattcaatc gaaatgaagg cttgaccgaa cttcaaattc ttccaagaaa acgttaaatt
     2581 atcaacagga tcaatttgca atactccttt tttgaatttc aatccgaaat attgactgat
     2641 cattaggctg acaaaaagac ccgatgccca gccgcatttg ccgcagccaa agtctcgcac
     2701 gacttctcct tgttgagcct ctaagcgata cggccaccac caccacgaac cgtctaaatc
     2761 tgccaaacaa attagttctt ctaatcgggt tacgaaagct tgagggttgt gcagctcgct
     2821 cattaaaaca gtgataaagc caggaaaagt ggcgccggat tctaatcccc aagtgatgcc
     2881 tttgatttcc tcgctatagg ttggattgaa agaactagca gaaaaggcca tggtgttctg
     2941 atataaatga tctttattat ctaaaaattg ataaaaaggc atcaacgtag tgtctgattc
     3001 ttctccatca tgcatcaata aattaatttt tccatccaca atcacatcac ttaaaaagat
     3061 taacccttgc tcaagaatcg gtttttgata gtgcttcact gaataggttg ttttctcctc
     3121 atcaccgatt ccctccaaaa attgctcacc aaaaggacca tcgatagtca tttgtttaca
     3181 aattgattcc tttagtttac aggcaatgtt tttgtatcgc ttgctaagac tcagttgttc
     3241 tagggcttca aaaatttctg caagtccaat acaggccttc caaagacaaa gattgctgcc
     3301 ggtatgatac tttccaagtg caaaggcgtc tgataaccaa acagatggaa aaagcattgg
     3361 ttcccctgac cgacgttgag ctaatatgtc ctcaataatt cttgaagcat tcagtagaac
     3421 ctgcggacac cgctcaaaga attttgtatc cccagtatgt tcaaaataaa gactagccag
     3481 taacgcactg gataacgaat tactcagaga atgattgata cctcctgtaa attttgaacc
     3541 aggaaactta acgccatatt gatcaaacca tagaaccgtt ttttgacata actccggttc
     3601 aaacatggtg aatggcaatg atgcgtaata catatcctta ttccaaattc gattagtagc
     3661 aggatagctg ccccaattgc tgccgacgac ctgtccctgt cgattcatac cgaaactaga
     3721 aaagctttga taaagtgcac gacgatagag atggacaact ctatcgtttg gcattgacag
     3781 ctgcccaaat agctgagcaa aatagttcaa tgtttccttc agccattttt cgatatcaga
     3841 ttcctgaaaa gtcattactt cttcaaaagc atttgggtcc acaaaagcaa tggcgaagct
     3901 tgccacttcc tgttgtgcga tcgtgtcctt atttcctttt tgagaaatca acacattttg
     3961 ctgatccgaa tacttctctt gatacgaagg cacatctttc actgaaactt gaaggctttc
     4021 gtgtgtttca ttcacaatgt aaacctgttg aacaagcata cttaaacgct tattagccag
     4081 tatgggagca aaggatacta caatgacctt aaacgtcgaa taatgatgaa tatatctagg
     4141 caaacagtct tgaatcacat cgacttcaat acgatgatcc gattctgaca aatggtagac
     4201 aatcccgttt acttcaacct ttaaggataa cgaattggtt tgagagagct gtttattgac
     4261 ccaaacacct ggtttttcct cagttagtaa attttctcga taaaaggtca acgtttgaat
     4321 cgtcccgcaa acatcaaggt ttgctaatgt cttttgatta cttacatcga aggtcaatcc
     4381 ctttacatca ggaatattcc gatagtagct gtattcgttt gaagtagtca catttccgtc
     4441 gtttaactca gcattaaaaa atgggtgttt gggtgaataa attacttcat taatagtcat
     4501 cttttccctc cttgctaaaa aaactgcagc ctagtagctg cagcttccta ctatgcgatt
     4561 ccaagtaatg acaataaaat tcccaagacg accattccaa aaataatttt gatgacgccg
     4621 acttttttat ctagtaattt gaaacagctc aatgttacca ataacggtac aattccttta
     4681 aaaatactat cgaggatttc ttggacattc attagttctt cgcccttcat tgaaaagctc
     4741 aaaacggtat tgaatttgac catactgatc gtcattgcac ctaccatgat cagacccaag
     4801 atactagcag cttttgtcag aatttcaatt aacccgttcg catacagctt ttcaatatat
     4861 ttagagccta atgaatagcc taaaacgccg ccatagtatc gcatcaagta attaggtata
     4921 ttgaacataa ctaaaaagat aattggtgcc aaaatatttc cggcattggc taagttgatc
     4981 gctagtcccg cggtaacgac acgccaaact ccccagaaca atgaatcgcc aattccagcc
     5041 aatggtccca tcaaactcgt tttgatcgcg ttgatacttt ccacgtcaaa accagctttt
     5101 tcagaatttt ccttctccat cgatgccgca atccccatga caaaggacgc gcaaggaatg
     5161 gtcgtcgaaa aatactgtgt atgacgttcc agcgccgcag ctcgatcttc ttcacttttg
     5221 tagaaatgat taatgaaggg catcatagaa taggcgaaac caccgccgcc catcgtcacc
     5281 ggattcaccg aagcatacat agtcatcgag cgccaaaaca tcttccgaac caattttttc
     5341 tcatctttag aaattccatc agctaatttc attgaaagaa atcctcctct tcttggtcaa
     5401 gcgttgcatt tacagcagca tcgctttgaa ctgcatgaac agatatattt tttagttgta
     5461 gatctctttg ggctataaca attgcaataa tcgttccaac tgcggcaatt gcaatcaatg
     5521 gtaactcgag atacgccata acaacggccc caaagaaaaa ccacacagca atatttttac
     5581 tccaaagcat ccgtaacagc atcgccatcc caactgccgg cagcagattt ccagcgactg
     5641 ttaatccgtt catgatgacc tctggaatat tatccaagac gcttttgaca acatctgctc
     5701 caaataaaac ggcaaagaat ggaataagag cataaagcgc atagtggaca aaccacataa
     5761 agaagtgcag attgatgatt cctttttgat caccagattt tgcaaatttg aaaaatttag
     5821 cagccatcgg tccacataca aaagaaaaaa tcatattcat taactgtaat cctaatactg
     5881 ctagtggaaa gacaatggga ataatcgcat ccacgtttcc tcccattttg attgcgaacg
     5941 ctgtagccag cccagcagcc aaagaaggct ccgccgtact agagattccg atattgacga
     6001 cacccataaa gatggcttct agtgacgccc cgacaatcaa ccccgtctgc aaatccccca
     6061 tcaacgctcc tgctaacgga gcaacaacaa tcggacgact cagcatggac atcccgaaca
     6121 tttcatgtcc agcaactcca ataaaaacgg ccaaagcgac tacaaatgca tcatacataa
     6181 tttttctcct tcctttttct aaaaatcact caaaatagag atagctgaac gtttttccat
     6241 cgccggtgta ggctgaaaaa cggtatctgg ataaagttca actaattctt ttaagtcttg
     6301 aatttctgac gtcgttaaca tcacttcctt tgataaggtt ttcttatctt ccgatggcgg
     6361 tgtcattttc ccagcattcc ccacgttcat taattcgatt tccgttggga aagctttcgc
     6421 aacttttacc gcatctttga cattagggac taaaaccatc aagcgcatgt ccttcgcccg
     6481 cggatctttt aacaaatgaa tcgcgtcatc aactgtttta ataatgactt tgacattcga
     6541 tggtgcagcc atcttcaatg tcatcttttg aatatcgttg gtagccgcct catcgcttgc
     6601 tactactaac ccggttaaat ttagactctt tgtccaagtc atcgctactt gtccatgaat
     6661 taatcgttca tcaattcgca ctgctttaat catttttctt cctcctaaaa aaaatcttcc
     6721 tcttcattta ctacatgtac ctcatcaaga atggtcatct ttaattcctg ttggcattta
     6781 tttaccagtt cctgaatatc ctcctttgtt aattccttgg ttgccaatac tatttcgagc
     6841 agaattggca gattaaagcc agacaaaata gttacagcaa tctctttctc caaaaaggtt
     6901 ttggtaatct tctgattgac acttccgcca aataaatcgg taaaaacaaa aatctgatcc
     6961 tgctcgttca cctggtttac aaatacattc aactcctgtt caaaatcaca ctcatccaca
     7021 taagcattaa tcaccgtaat ctcttgaacc ttccccgtca gtaattgaat actgctaaga
     7081 aagcctgaag ctaagtgacc gtgagtcgct attagaattt tcttcatatg gttaattcct
     7141 ttctttctgt tcacttttat ataagcaatt cctgtgccaa cttttaaaaa cattggttta
     7201 atcacgttaa ttcacattaa aaaaagtgtc taacctaaaa aaaacagatt agacactttt
     7261 ttagacactt tatttttgaa tatacggtcc taataattca tagagcagcg aaagctcata
     7321 agcattgact ttaaaattat atttcatctc cattgattga aaaatacttc tagaaacata
     7381 ataaaattca tcttcatctg gtttcaaatc ataattcggt tcttcagatt tttctgtccg
     7441 agccaagaat aaacgttcca tcatcaatgc cgtatgcata tagagattta gttttacacg
     7501 gccatttaaa gtgaaatgat agtaattttc atatttagaa atgactgttt ccacttcact
     7561 catcacgatc gttgggttca gaaagttcaa acggtccgca attccttcca acgaaaagaa
     7621 ttttactagc tcctgaatca agcggttaaa ttctgccggc ttcatttcag gactcaaaat
     7681 atgctccaaa ttggctgctc catcaccgtc taagatgtca taaacattaa tattaggaat
     7741 tgaaaaggaa cgtggaagat ccgaagtcgt gatgaccaat tttgtcttgt caaaataagt
     7801 ttcatcattt tggtcgatta attcacgtag ttctctgtag tccttcgtga aaacttcgat
     7861 ggattcatcc gataaatgat gacgaaaaac atctttcaat ttgtccgaaa tccccaaacc
     7921 cgacatacag gaaatgataa tgttgctact ttgagaaaag ccttcaaagt attgaacatt
     7981 gattgaatac ttgtctgctg catcctccgc aatctgttta aatggcgcat tcatcgtcat
     8041 cttcaagcca atatcaagtg caatggaagt cgtcaaatta ttcagaagaa gtaactcccc
     8101 ttttaaatcg ttcttgatct gtgtgtaaat ttgatttaaa gagcccatat ctacgatcag
     8161 cactagacta tctgtattca cttgccgttt cacgaaattc cttgtcgatt cgacaatctc
     8221 tgacacatta ctctctactg gcatatccaa cgcttcaaaa acataattgc cgcataattg
     8281 attcactact gcttgaatgc tgctagccgt actatctccg tgagcaagca acaggccctt
     8341 cagctcgatg gattcatcta cataatcaga taaagtcacg acaataagca gtcttagaaa
     8401 gttctcgtcc ttttctgaga gcggtggtaa atgctccaag aactgattac ataagtaagt
     8461 cgtccgaggt agatttgcag ctaatgtacg atttatcccg gttaagtctg tcttatagct
     8521 tggggaaatg acataggatc tgctaataat ttctgcaaca ggtccactcg tcgtcaatcc
     8581 gtacgttttt cctaaaacgg atacccagac ctttctaaac atctgaatca tcaattttct
     8641 actcattgat aaaaaagaat ccttatcaat gtgatgcacc aaatgctgta cttcattctt
     8701 tatcgaagaa aaattttcat tgaaattacc ttcatttatc agctgagtaa ttttttccaa
     8761 aatcgaatca taatcaaacg gtttgattcg atccgttttt atcttttgat ttttctttat
     8821 caataaacta gcaatcggca agctttctac ttcggattcc agcatattta actgaatcaa
     8881 gatctcattt ttcccaatcg ttaatggttc ttcttgtgtt cgactataca catccgcaca
     8941 actgatttta atgatattct tcagcttacc aatattccca ctaaagggat gagataacat
     9001 taaagacaag gcttctcttg aaacaataat atctttttgt aaaacatccg cttcattttg
     9061 aaaaaataat tgaatcagct ctaatcgttc tcgaatgggg cgatcttcaa aagttggcaa
     9121 cataacacta atttgaatac gccgatcaaa ggtgtctaat aaataatttt cacctttttc
     9181 cgtcgtggcg aaaataaaac gaaccttagc agaatgccaa tttacatttt caccgactgg
     9241 acgatagttt ccagtatcaa taaatagaaa gagcttttct tgattttctt tcgataggcg
     9301 atggacctca tcgagaaaaa gatagccgtt gttggccgtt gccaataaac catcggtatc
     9361 cttttcagcc cccgtaaagg cccccttctt gtatccaaac agtgttgccg aaagcagttc
     9421 gggattgtcc gcataatcgg cacagtttaa gatagcaaat ggtgcatctt tttcaataat
     9481 ttgctcatgg atagcgtatt caaaaatttt tgtggctaaa aaactttttc caacacccgt
     9541 ggctccattt attaatacac tgagtccgtt aggcggatat ttgacagcgg ctatacattt
     9601 ttgaatgacc tctctcagac ttccgtcatg accaactaca gaatgaaagg cttcgtttat
     9661 ttgctccgta ttatctacaa ccgcatattc atctaaaggc ttccacaaaa caggctttcc
     9721 cggtaatttc tcgacctttt tatcttccac taatctcgtc aaataatggc tggcattctg
     9781 acgagagata cctagattct tcgaaatttt ctcggcagtg acagaagaca tattccctgt
     9841 tagtcgtatc agctcatcaa aaactcgatc tttagcgctc tttttcattt tttcctccag
     9901 tttaatgtgt ctaaaagcat aataaactat cggctgcaat atgtctattc tatatttgta
     9961 ttccaatttt tactttgaaa cacaaatagt cgaatatttg cctcagatat ctgacaaaat
    10021 aatttatcta ccaatttagt ttttatctaa tatgattttt atgttttttc atagctacaa
    10081 atattgaact ggattcatat attagtttta ttaatagatt acagaagggt taagtatatt
    10141 tgcatagttt ccaattactt tatccttctg taactttttc aatcttagat ttactagccc
    10201 gtctcgggcc ctattttaca atggttttgt ccacttttct ctggtagaaa aacgtggtag
    10261 aaaaaataaa ataacacaaa aaaagcggcg tttcatccgc ttttggtaga agattggaaa
    10321 actcagatag aaaaaggacg atttttagct ctttagaacg ttgataagtc cccattctta
    10381 ctgagttttg ccattatctc ctatacatct tgaattctaa gaataggtca ttatagatgc
    10441 ctaggtattt cgatcctaga tctttgtaga cttcaagatg ggagatcgtg tcatcagatg
    10501 gatagaattg ttcgtccgac gaaatgtctt ttggcaataa cgcttttgct tcttcatttg
    10561 gtgtagaata accaatatac tctgcatttt gtgccgcatt ttccggttct agcataaagt
    10621 tgatgaaatc ataggcacct tctttatttt tcacggtttt tggcataacg atattatcaa
    10681 accaaagatt ggatccttct ggtgggatca cataatgtaa atgttcattt tcccacatca
    10741 tatcggcagc ttcgccagaa aatgtcactg ccacggaggc ttcttcattg atcatataca
    10801 ttttgatctc atctgccacg attgctttga catttggtgt taaatgattc aatttatttg
    10861 ctgcttctgt cagctgtgcc atattcttac tattcaaaga atagcctaaa ctattcaaag
    10921 aaagccccat aacttctcgt gcaccgtcaa tcagcatcaa actgtctttt aattccggct
    10981 tccacaaatc atcccaatgc ttaatttgat ttgcatcaaa gaatttatcg ttatatacga
    11041 tccctaaagt tccccaaaaa tatggaatcg aatactgatt atctggatca aaatccaagt
    11101 ctaaaaatcg tggatcaata ttatttaatc ctttgatctt cgaatgatct aatggaagta
    11161 ctagtttttc tttgatcatt ttttggatca tatattcact tggtatagcg atgtcgtagt
    11221 ttgtcccacc ttgctggatt tttgtgaaca tcgcttcatt agaatcaaat gtttcataat
    11281 tcactttgta tccatattct ttttcaaatt tcgtgatcaa actaggatcg atataatctc
    11341 cccagttata gatggtaagt acctttgctc cactcatgcc acttgttttt tcaagttgac
    11401 gaacaccaag cagcagcacc gcaataatga tgagaatacc taaaaacaga gattgtaatt
    11461 ttttcatcgc agccgtgcca ccccctcgcg tactcttctg tttttcttca gccgttttgg
    11521 acggttttct ttactaataa agtagtatcc aatgaccaat atcatggaga acaagaaaac
    11581 taacgcactt agcgcattga tttctaaact gattccttgt cttgcacgag aatagatctc
    11641 tactgagagc gtcgtaaagc catttcctgt gacgaaaaat gtaaccgcaa aatcatcaag
    11701 agaataggta aatgccataa aatatccagc tatgattcct ggagaaagat aaggcaaaat
    11761 gatatttttg actacttgga aattattagc accaaggtct ctagccgcat cgaccattga
    11821 atcgttcatt tcctgcaatt ttggcaagac catcaagacg acgattggaa tgctgaaagc
    11881 aatatgggaa agcaatacac tctgaaaacc taatccgaat cctagtagtg taaagaaaat
    11941 caagaaacta gcaccgataa taacatcagg agagactaat aagatgttat tcatgctcaa
    12001 caacgctgtc cgtgcacgtc gttttttcgt gtaatagatt cccatagccc caaatgtccc
    12061 gatgatcgtg gcgatcaatg cagataagaa agccagcatg aacgtattca atacgataat
    12121 gatcaaacgt gtatcttcaa aaacagcttc ataattttcc caagtaaatc ctgtaaagct
    12181 gttcatcgtt cctccttcat tgaaggaata aaaaatcaga tagaagatcg gtgcatataa
    12241 gaataaaaag acgatcccta aataaagata cgaccattta aattttttca tttatgagcg
    12301 acctcctttt ttcttctcgc ctgtcaggag catcacgatg aacatagcaa tgattaaaat
    12361 cacaccgatt gtagaaccca tcccccagtt ttgtgtaacc aagaaatgct gttcgatagc
    12421 cgttcctaaa gtgatcaccc gatttccgcc aatcagacga gtcagcatga ataaagaaag
    12481 agaaggaata aatactgctt ggacaccact tttcacacca tttaatgata acggaaaaat
    12541 cactcgtcgg aaggtctcaa agctattcgc gcctaaatct ctactagcac tgatcaaaga
    12601 aggattcatc tcttccaatg cattgaaaat tggcataatc ataaatggaa tctcgatata
    12661 cgttgccacg aacagaaaac tgaagtctgt gaataatatc tgtttcggcc cgatcccgat
    12721 aaaatcaaag aaatgattca cgctgccgtg cacactgaaa atcccaataa atgcgtaagc
    12781 tttaagcagc agattcaccc atgtaggcaa aataatcagc atcagccata attgcttgtg
    12841 cttcagtttt gttaaaaaat aagcagtcgg gtaactgatg atcagcgtga aaaacgtgat
    12901 caaaaatgca taccatacag aattgatcgt catagaaagc gatgtccccg acgtaaaata
    12961 agatagataa ttattcagcg tgaattgccc attcatatca aaaaaagatt gataaatgat
    13021 cattaataca ggcgcgatca cgaataaacc gagccacaaa atataaggga cggaatagat
    13081 cctgcgcatt gttttcattc ttgtcccccc tattcttcgt agctgtcaag acgtgcatcg
    13141 aattcttctt cagactcgtt gaaacgcatg acatggatat cttctggctc aaaggccaat
    13201 cctactttgc ttccttcttt agcttttttc gttgaatgga ccatccattc attttgctgt
    13261 tcatcataac agatgatttc ataatggaca ccacgaaaca gctgagtatc gaccgttacg
    13321 actagttttc ctttatctgg tgtcgtgatc gtcaaatcct ctgggcgtaa aacaacttca
    13381 actggttcat ttagacgcat cccgccatcg acacattcaa attgcttacc aacaaactca
    13441 actagattat cttcgatcat tacaccgtcc acgatattgc tctccccaac aaaatcagca
    13501 acgaaatgat tgattggctc atcataaata tctacgggtg taccactttg gacgattttc
    13561 cctttattca tgacaaaaat ctcatcactc attgccaacg cttcttcttg gtcatgagta
    13621 acaaaaataa acgtgattcc taatcgttgt tgcagatccc gcagttcata ctgcatatct
    13681 gtacgtagtt tcaaatcaag cgccgataac ggttcatcta atagcaatac tttcggttca
    13741 tttacaattg ctcgtgcaat cgctacacgc tgtctttgac caccagacat ttcactaatc
    13801 tcccgttttt cgtatccagg aagctggacc atacgcaaag catcttttac cttcttttcg
    13861 atatctgatt tggacatttt cttgatcttc aatccaaaag caacgttatc aaaaacattc
    13921 atatgaggaa aaagtgcata atcttggaag actgtattta cttgtcgctt attagctggc
    13981 aaatcattca accgtttccc ttcaaaatag atgtcccctt ctgtagcttc ggtgaatcca
    14041 gctataatac gtaggatcgt tgttttacca cagccggaag gaccaagcag tgtataaaat
    14101 tttccttgtt cgatctcaaa acttactttt tttaatactg tttcatcatc atagcgtttc
    14161 actacttgat caaatgaaat gattggtttc cccacgtttt taccccctat attacaaata
    14221 tgattgcgtt gcaacaatca atacttttgt tcgttttttg agatgattcc ttaattgatg
    14281 ttgttttgtc gcttgataat agatcgactg tcctgctttt gccacataga ccgtctctcc
    14341 taagcttaac tctaattccc cttctaatac aaaaataaac gtttcagata atgaaggttc
    14401 aaacgtctta tattctcctg caccttcaaa agtcagcaag acaggctcca tttctttttc
    14461 attcgaatca gccacgagcc attccaattc atacccgttt tcttcatcga aataaaccgt
    14521 atgatcttct tttgaataaa ccacttgcag attcgctgct tcttgatgaa aaaattcttc
    14581 tggagtcaca cctaagactt caagaataga aaagaatgtt tccattgatg gtgaacttaa
    14641 atctcgttcc aactgggaaa tatacccttt tgacaaatct gtgcgttcgc ctagttcttc
    14701 ttgtgtcaaa ttcttttgga tgcgcaaatt tcttaacttc tcaccaattt ccataatttc
    14761 ctccgaatcg aagttttcta atagtaaact tcctgtttac taccactcgt attagtatac
    14821 gaaatttatt gaaaaaggca accctttgta aaattttttt tgctgaatat ttctttatct
    14881 tttggaataa attctagttg tttgattctt tatttttcac ctaatttttg tctaaattta
    14941 cggaagaaca agaataaaag agtaggcttt atggtatgat aaaagaaaat tttaaaagga
    15001 gtgtctcacg aatgaacgct tttcgatctt ttcaggtaat aaaggaacct gttttccata
    15061 cagcaatcag tgatctgcca tttactgagt tggctgaaaa tgaagtcttg gtaaaaattg
    15121 cctattctga tgtcaattat aaagatgcat tggcaatgag cgagtctggt caggtgatac
    15181 gaacatatcc gatgactcca gggattgatt tatccggaac tgtcgtccaa agtaacaatc
    15241 cacgtttttc aaaagaagat cttgtacttg ctactggttt tgggcttggc gtcacccatc
    15301 caggcggtta tagccaatat caaaaagtac caggagactg gttagttcct ctaccaaaaa
    15361 atatgacgct gcgtcaagcg atgatccttg gtacagcagg gtttactgct atgttgtgtg
    15421 tgaacgcttt aatccgtcaa ggtatgacct ccgataaaaa agttgtcgtt actggtgctt
    15481 ctggcggtgt aggcagtaca gcgatagccc tgttacataa actaggattc cagtcaatca
    15541 ttgccttttc cagaaaagaa gagtctgtca catggctaaa aagtctaggt gctttgcaag
    15601 tcgtacgacc tgatgaattt ctgccagaga caacaaaagc tttaggaaaa cagcaaatcg
    15661 actatgtcat cgacacagta ggtggcgagc agctaagcag tcttcttcca ttgatctctt
    15721 ataacggtgc agtagcttta tgtggcaatg ctgggggaat caaactaaac gcgactgtct
    15781 tgccttttat tctgcgaaac atccagttga ttggaattga ttctgtcaat gtaccaatag
    15841 atcaacgtct ttctttatgg caacagatgg cggatttgca gattgcagat gaactggtcg
    15901 tccaagagat tactctggat caattgcctg aaacagcatc taaactactt gctggaacac
    15961 accaaggaag aacactagta aatgtaggtg accataaatg aagattctaa tcacagctgg
    16021 aggaacgact gaaaaaatcg atcaagtacg tgctattaca aatcattcga caggacgatt
    16081 agggcaagca ttagctgatt atttagctgc caatccggat accacaatcg attacgtgac
    16141 tacgaaacaa gcactgaaac cgaaaagaca ctccaatata acgatttata cgatcgaatc
    16201 tgcactggac ttatttttac aattagaaga actaacgaaa aaagaacact acgatgcgat
    16261 tatccacagt atggcagtca gtgattttac tcctgctttt agtttttcag aagaacagct
    16321 ggcaaaaaac ctacccactt cttcaacgca agaagaactg gacaattggt ttgcggaaaa
    16381 tgaacagaca gataccactg tttcaaagat ctcttctaac accgagcatc ttgtgctcgt
    16441 tttgaagaaa acaccaaaaa tcatttctta tctacgtgaa tggcagccaa aagcaaaaat
    16501 cattggtttc aaactattag tagatgtacc aaaggaatct cttctggcag tcgcaaaaaa
    16561 tagtctgata aataacaaaa ctgattttat tttcgcaaat gatttgacgg aaatccatgg
    16621 cgagacacac cacggctatc tcttatctaa agatggaaca gtggaagaag ctcaaagtaa
    16681 atctgagatt gctgcattga ttactgagaa aattagactg gaggaatcaa aatgaaaaag
    16741 atcttactag gcgttactgg aagtatttct gcttacaaaa gcgctgatct tacaagtcaa
    16801 ttcgtcaaat taggtgccca ggtagatgtg attatgtcta aaagcagcac acattttatc
    16861 acgccattaa ccttgcaatc tctgtcgaag cgcgcagtcc atacagacgt catgcaagaa
    16921 tcaaatccgc aggtaatcaa tcatatcgaa ctagcaaaac aagctgactt gtttctcatc
    16981 gcgcctgcta cagcaaacat tatcggcaag ctagcaaacg gcattgcaga cgatcttctt
    17041 tcaacagtcg caatggcgct tccagtggga acaccaaaat tgattgcccc tgccatgaac
    17101 acgaatatgt atcaaaaccc gatcactcag cgtaatctgg aaacattgag atctgtcggc
    17161 tatcaggaaa ttgaaccaag agaatctctt ttagcttgtg gagattttgg aaaaggtgcc
    17221 ttagctgata ttcagacgat catcgaacag gtcatgctga ctcttaatga aagaaatgct
    17281 tagaaaaaag gagtacttat gaaaaataca aaaaacttta cattaactgc gatgttttta
    17341 gctattttga ttttattagc tgttacgcct ttaggtttta tccctatcgg tccaatcaat
    17401 gcaacaacca tgcatattcc tgtaatcatt gcttcgatcg ttttaggacc aagactaggt
    17461 gcttttcttg gcggtacatt tggactgatc agcatgatcc gtagcacagt tatccaaaca
    17521 cctttgtctt ttgtcttttc tccgtttatc ccagtcatcg gaacagaaca tggaagctgg
    17581 aaagccttgc tgatcgcgtt tattcctcgc atcttgattg gggtggttcc ttatttcgtt
    17641 tataaaggtc tgcgcaaact aatgaaacaa aaagcagatt ccgtttcttt attcttagct
    17701 ggactttccg gttcattgac caatacgatt ttagtgatga atatgatcta ctttttattc
    17761 cagcaagatt atgccaaagt catcggaaca aatttaaatg ctgtttattc agctattcta
    17821 gcggttattt ttacaagtgg tatacctgaa ggaatcgtcg caggtcttgc gactgcagca
    17881 gtatgcaatg tcttgttaag atacttcaag cacccttctc ttactaactg atagtcatcg
    17941 tagccgatct ctgatttttt tctcttttgg tgttactatt gaatggaaag atttgacgtc
    18001 gaaaggatga tccaaatgaa aatttttgtt gtaggtgcaa acggacaaat tggccgacac
    18061 ctgataaaag atttagctcc aagttcccat gaaatttttg caggggtgag agatgttgct
    18121 tcccaaacat tagtaaaaaa agagaatgtt tcgtatgtct cttttgattt aacttggtcc
    18181 gtggaaaaaa tgaccgaagc attcaaaggc atcgatgtat tgatttttgc tgccggctct
    18241 caaggaaaga atcttctgca agtagacttg gatggcgcaa tcaaaacagt gatcgcagct
    18301 gaaaatgcgc atgtctctcg ctatctgatg gtcagtgctg tttatgcaga tgaaccagca
    18361 aaatggcctg agtccatgac agattattat attactaaac attatgcaga tgaatggctg
    18421 aaacgaacta acttagattt tgtcattctt caaccagtca cattgactaa cgatgaagaa
    18481 gttacttcca ttcaattaac taagcctaat gagaaggcat caaaaacgat cactcgctct
    18541 acagttgctg ctgtacttgc cgctctagtg gaagaaacgg atatttctcg tacgactcta
    18601 gtactttctg aaggtagcaa agagttgaat acagcattcc aagaatgggc aaaggaggaa
    18661 tagatccatg cgtgccattt caatcaagca tcctggttct gtagatgtat tagaacaaat
    18721 tgaagtgcct gttcctaaac caaaacaagg cgaattactg atcaaagtcc atacagcagc
    18781 tgtcaatcga acagatatta tgaaaagaga atcagtctct ttgaaacctc cctaccctat
    18841 tttgggagtc gaagtagctg gagaggtagt tgagaataaa agtgatatct ccacttttac
    18901 tcctgggaca aaagtatacg gattagtcaa tctaggtggt tatgccgaat atgctgtgat
    18961 gcctgccgat cgtgcaatcc ttctccctga tacgctagac tatgtttctg ctgctggaat
    19021 ctcagaagtt tttttgactg cttatcaaac actttactgg cttggaaaat tacaagaagg
    19081 cgaaactgtc ttgatccatg caggagcaag tggtgtggga acagcagcga tccaattagc
    19141 tagacagctt tctcacgcga aaatcatcgt tactgcagga tcagaagaaa aactcgcctt
    19201 ttgtaaagaa cttggtgcag atgaattgat caactataaa aaacaagact ttcaagaaga
    19261 ggtacgaaaa atcactgaag gtcggggagt cgatgttatt ttggatttca tcggcgcttc
    19321 ttactgggaa aagaatttag cttctatagc cgttgatgga cgttgggtct tgattggtat
    19381 gctaggtggg accatcgtgc ctgagataga ccttggtgta ttgattggga aacgtgtcca
    19441 actgcttggt acattattga cacctagaag tgatcagtat aaagcagagc tcgcacaaac
    19501 ttttatgcaa aaagctgggc cttttctaga aaatggcaag attcggccaa tcattgaccg
    19561 tacattccaa ttcagcgcag tcaaacaagc gcatgaatat atggaagcaa acaaaaacat
    19621 cggaaaaatc attttgaaga taactgaata aaatcatgct gatcaaaaaa gaggctggga
    19681 cagaagcgtt taactccaag aaataagaag aaattcacga aaattgcttt tcaaattttt
    19741 gtgaatttca gcttatttcc gaaggaattt cttctgcttc cgccgtttat acgtgtttag
    19801 agcgtgagac aaaagtgttc tttacttttg tcccacgctc tttttccga
//
LOCUS       DACECZ010000040        19818 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.040, whole genome shotgun sequence.
ACCESSION   DACECZ010000040 DACECZ010000000
VERSION     DACECZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.040"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            257..532
                     /locus_tag="IWO35_001364"
     CDS             257..532
                     /locus_tag="IWO35_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316426.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2617
                     /locus_tag="IWO35_001365"
     CDS             545..2617
                     /locus_tag="IWO35_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0316427.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2782..4668
                     /locus_tag="IWO35_001366"
     CDS             2782..4668
                     /locus_tag="IWO35_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0316428.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4680..5627
                     /locus_tag="IWO35_001367"
     CDS             4680..5627
                     /locus_tag="IWO35_001367"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0316429.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5647..6174
                     /locus_tag="IWO35_001368"
     CDS             5647..6174
                     /locus_tag="IWO35_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0316430.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6237..6890)
                     /locus_tag="IWO35_001369"
     CDS             complement(6237..6890)
                     /locus_tag="IWO35_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0316431.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7022..7864
                     /locus_tag="IWO35_001370"
     CDS             7022..7864
                     /locus_tag="IWO35_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0316432.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8022..8918
                     /locus_tag="IWO35_001371"
     CDS             8022..8918
                     /locus_tag="IWO35_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0316433.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8921..9634
                     /locus_tag="IWO35_001372"
     CDS             8921..9634
                     /locus_tag="IWO35_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ0316434.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9654..10172
                     /gene="msrA"
                     /locus_tag="IWO35_001373"
     CDS             9654..10172
                     /gene="msrA"
                     /locus_tag="IWO35_001373"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0316435.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10169..10396
                     /locus_tag="IWO35_001374"
     CDS             10169..10396
                     /locus_tag="IWO35_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ0316436.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10548..11099
                     /gene="lepB"
                     /locus_tag="IWO35_001375"
     CDS             10548..11099
                     /gene="lepB"
                     /locus_tag="IWO35_001375"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0316437.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11182..11724)
                     /locus_tag="IWO35_001376"
     CDS             complement(11182..11724)
                     /locus_tag="IWO35_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0316438.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11961..12359)
                     /locus_tag="IWO35_001377"
     CDS             complement(11961..12359)
                     /locus_tag="IWO35_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0316439.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12619..13479
                     /gene="ylqF"
                     /locus_tag="IWO35_001378"
     CDS             12619..13479
                     /gene="ylqF"
                     /locus_tag="IWO35_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ0316440.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13472..14239
                     /locus_tag="IWO35_001379"
     CDS             13472..14239
                     /locus_tag="IWO35_001379"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ0316441.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14295..15152
                     /gene="dprA"
                     /locus_tag="IWO35_001380"
     CDS             14295..15152
                     /gene="dprA"
                     /locus_tag="IWO35_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ0316442.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15274..17352
                     /gene="topA"
                     /locus_tag="IWO35_001381"
     CDS             15274..17352
                     /gene="topA"
                     /locus_tag="IWO35_001381"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ0316443.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17321..18697
                     /gene="trmFO"
                     /locus_tag="IWO35_001382"
     CDS             17321..18697
                     /gene="trmFO"
                     /locus_tag="IWO35_001382"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ0316444.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18895..19800
                     /gene="xerC"
                     /locus_tag="IWO35_001383"
     CDS             18895..19800
                     /gene="xerC"
                     /locus_tag="IWO35_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ0316445.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT         6874 a         3414 c         4122 g         5408 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa
     2641 gttttataaa aaacaaaaca tatcagaaca ggatcgaatc ggtcctgttc tttttttgag
     2701 aacacaaaaa gaacaaatac caaaaaaatt ttcctgaagt tcactatttt tatgatacaa
     2761 tatccaagat aaggagggaa catgttgaaa gaattaaaag taactgacct caaaaaaact
     2821 tatggtgaaa aagatttgtt tgaccagatt tcttttttgg tccacgaaaa agaccgtatc
     2881 gggttgattg ggacgaacgg gacgggtaaa acaagtctct taaatattat tgccggtatc
     2941 gatagcagcg acggtgatcg gcaaacagtg ttttatccaa ctgattaccg tattggttat
     3001 ctttctcaag cagctgaatt ttccgatgaa ttaactgttc tccaagcagt ctttcaagga
     3061 aacagcccct tgatccaaac tgttcgagca tatgaggaag cattgatcga actaggggaa
     3121 aacggagaag accctgatgt gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa
     3181 gaagatgctt ggactacaga taccaacgca aagattattt tgcaaaaatt aggaatcagt
     3241 gaattagata aaaaaatcag tactttatca ggtggtcaga aaaaacgagt tagtctggcg
     3301 caagtgttga ttgatgaacc cgatttacta ttactagacg aaccaacaaa ccatttagat
     3361 tatgaagcga tcgagtggtt ggaaaactat ttgaaacaat accgtggcgc actattgatg
     3421 gttacccacg atcgttattt tctagataga gtagcaaacc gaatcttcga actttcattt
     3481 ggaaaattat acgagtataa aggaaattat gaagcttacg tcttagaaaa agcagaacgt
     3541 gatcgtgtag aagtagaaca agaagaaaag cgcaagcggc tttataaaca ggaattagct
     3601 tggatgaggg ctggcgtaca ggcacgtggc accaagcagc aagcacggat caaccgtttc
     3661 gaagatctaa aggagaactt gtaccaagta aaccaagaag atgatttaga gctgaaccta
     3721 gcgacacaac gtttagggaa aaaagtactg gaaatcaaag atggcagtta ccgtataaat
     3781 gaccaaacac tgctagaaca tttagattta ttgattcaat ctcgggaaag actaggtatt
     3841 actggtaaaa atggtgcggg aaaatcgaca ttgcttaata ttctagctgg cagaattccc
     3901 ctggatagcg gtacaatgtc tatcggtgaa acggttcatc ttgcgtatta tacacaagaa
     3961 aatgaagaaa tggcaccaga taaacggatg attgcttact tacaagaagc tgctgaagaa
     4021 gcaaaaacag cagatggcag tcaaatcgga gtagcagaat tacttgaacg atttttattt
     4081 ccaagattta tgcatgggac actgataggc aaactttcag gcggagaaaa acgccggtta
     4141 tttttgttga aactgctgat tcagcaacct aatgttttat tattggatga accgaccaat
     4201 gatttggaca tagcaacatt gacgattttg gaagattatt ttagaagttt tccaggtgct
     4261 gtgatcactg tttcccatga ccgttatttc ttggacaagg tagcagataa gttattagta
     4321 ttccaaggaa acggaaaaca agaactttat tacggaaaca tgtccagtta tttactgaag
     4381 caaaaagaga cccagcagcc tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct
     4441 gctgaaaaga agaaattatc ttacatggaa caaaaagaat gggaaacgat tgaagatgag
     4501 attgcagaac ttgaagaaaa aatctcattg ctccaagaag agatgaatca tcaaggagat
     4561 aatttcactc gattacaaga attgcaaaac gacgtatctg aaacagaagc acaacttgaa
     4621 gaaaaaatgg cacggtggga atacttgagt gaatgggtag aagattagga ggaaaaagga
     4681 tggaacaagc gtatcttgac ttaggaagaa aattgttgga agaagggcat cgtaaagaag
     4741 acagaacagg cacaggcaca ttaagcttat tcggatacca aatgcggttt gatctgcaaa
     4801 aagggtttcc tttgttaaca acgaaacgag taccttttgg attgatcaaa agcgaattgc
     4861 tttggttttt aaaaggagat acgaatatcc gctatctatt acaaaataaa aaccatattt
     4921 gggatgaatg ggcatttgaa cgctacatca aaagtccaga ctataatgga ccagatatga
     4981 ctgattttgg ccatagatgt ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga
     5041 gcaagctttt ttgcgagaag atcctaaccg atgattcctt tgctgaaaca tacggcgatc
     5101 ttggacacat ctacggctat caatggcgtc actgggaaac aaaagacggt ggctttatcg
     5161 atcaaatcaa acaagtgatc gaagcaatca aaaagactcc tgattctaga agattgatcg
     5221 tttctgcatg gaatccggaa gatgttccta gcatggcttt gcctccttgt catacgatgt
     5281 ttcaatttta tgttcaagag ggacggctaa gctgccaatt gtatcaacgc agtggggatg
     5341 tgtttctagg cgttccgttc aacattgcaa gttatgcgtt gctgactcac ctaattgcac
     5401 atgaaacagg tttagcggtt ggcgaatttg ttcacacatt aggagatgct catctttata
     5461 tcaatcatat cgaacaaatg aaagaacaat tgagccgaga aatacgcgca tttccgactt
     5521 tagttttgaa tcaggaaaag cagtcagtat ttgattttga gatggaagat atccaattag
     5581 aaggatatga cccacatccg gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat
     5641 gagaaaatgt tagctgcaat ttgggcacag gatgaaaacg gattgattgg aaaagaagac
     5701 cgtcttcctt ggcgtttgcc aaatgatctg aaattcttca aacaaatgac agaagcaaat
     5761 acactagtga tgggacgaaa aacattcgaa gggatgggta gccgtccgtt gccgaatcgt
     5821 cagacgattg ttttgacgag agattcttcc tataaagcag aaggcgtgca tgtcatgcat
     5881 agtgtggaag acgtgcttgc tctggaaaaa gaaacagacg gtatcttttt catcgctgga
     5941 gggtcagctg tttatcaaga atttctgcca ttttgtacga tattataccg tacagtcatc
     6001 catcatgtat tcgatggaga tgcttatttt ccacctgttg actggtctga ttggtcactg
     6061 atcaatacta gtcaaggaga aatggatgaa aaaaatcgtt atccgcatca atttgaaacg
     6121 tatcagcgta atgagaatcc tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc
     6181 tatggataga ctagctgtta tttagagctg tcccatccat agctttattt tttttattaa
     6241 gtaaagaata aaacagagaa gtacataaaa cctgcaccta acataacaaa caaatgccaa
     6301 acaacatgca tatagcggat agaattcaag ctataaaaga atgcgcctat cgtgtaagaa
     6361 agcccgcctg ctaccatcaa aataacacca gtcattccaa gagagtgata aagaggaaca
     6421 gcagccacga cacagagcca ccccataaaa atatagatga tcgtagatac ttttgatact
     6481 ctctcccgtt tatgcaacgt caacgcttta tatataatgc caagaatcgc aaacaaccag
     6541 attaatacaa ataaagtcca acctagccat cctcggatac tcagcaaaca aaaaggagta
     6601 taacttcctg caatcaaaag aaaaatcgaa ctatgatcga atacttggaa cacctttttt
     6661 gcttttgtaa aaatcaatga atgaaacaaa gtagacgtta aaaataaaag aatcatcatc
     6721 gatccataga tagcataaga gacaatatgg agggaggaat gcaagtgggc accttttact
     6781 aaaagaacca ctagtcccgc gatactcaat ccagtcccaa ttccatgggt gactgcatta
     6841 aacacttcat ttaatatttc atacgttcga ttaaatttcg ttttttccat gaaaacggct
     6901 cctttactag gtgtaattaa tgaaaaatca ttatttgtct gttatactaa aaatagaaat
     6961 tgtcttacat gtagtttaac atgaataagg catcttgtta aagaaagaga gagagaaaat
     7021 aatgacaaat gtaaaaatcg taacagattc ttcatgtacg atggaaaaaa atgtacgcga
     7081 tgctttaggc attcgtgtca tgccgctatc catcatgatc gacggaacag tatatcccga
     7141 tgatgaccaa ctagacggcg aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc
     7201 caaaacaagc caaccaccaa ttggagaatt catctcgatg tatgatgagc taggagaaga
     7261 cggcagtgaa gtgatttcga tccatatgac aaaaggcttg agtggaacag tagaagcagc
     7321 aagacaagcc agtaatctaa caaaaacgaa agtaacggtt atcgacagtg ataccactga
     7381 ccaaggatta tcttttcagg taattcgagc agctgaatta gcaaaagaag gtaaaacagt
     7441 agagaaaatc cttgaagaag tcaaaaaagt acaagacaat acaaaattat acatcggaat
     7501 atccacacta gataacttgg taaaaggtgg aagaatcagc cgaacaactg gtttgctgtc
     7561 tagtgttttg aacatacgcg tggtgatgaa tttcgcccat tctgaattaa tccctgtaac
     7621 taaggggcga ggaaagaaaa cgttcgataa atggtttaat aatttgaaaa atgaactgaa
     7681 agaattgcca aatgtgcgac aaatcggtat ttcacatgcg gatgcaaaag aattggcgca
     7741 agaattcaaa gagggtttgc aagaattatt cccggacatg catatccctg tactgcatac
     7801 gaatccggtt atcgctacgc atactggtaa agacgcgttt gcaatcatgt attatgtaga
     7861 ttagtcgaaa agaggataag ccctcagcct gaaaggcttg cagcttattc tctttttctt
     7921 tataaaatta ggaaaataat tgttttcttt tttcataaag actacctttt tgataaaatt
     7981 tttgataaca ttagaaccaa tcgaatacaa gaggtgaaaa aatgaaatta acgaaacgac
     8041 atttgatcct cgttttgttc ccattattaa ttgcgatcat catctatgca ggtttagctg
     8101 cttctatacc gaaagctggc ccactaatga aaccagtaac tactgtgaga gaagatccaa
     8161 ataaaaaaga aaaacttcac tacatagcta tcggagattc gctgactgaa ggagtaggag
     8221 atcaaaccaa gcaaggagga ttcgttcccc ttgtcgcaaa tgatctaaaa gaccgttata
     8281 atttgaccgc ggttgaaaca gaaaactatg gtgtaaatgg tgaacgaagt gaccagatct
     8341 taaagcgagt aaaaacaaaa gaagaaattc gaaacaatat caaaacagct gattttatca
     8401 cactgactgt tggcggtaat gatttgatga aagttatcca aaataattta ttcgggttaa
     8461 cggtcaattc ctttaaaaag ccactgaaaa actatcagga aaatgtcaca gaactattgg
     8521 aagaatttcg atccctcaat aaaaccgcac ctatttacat tttaggcatc tacaatccat
     8581 tttatttgaa ctttcctgag atcactgaca tgcagacaat tgttaacaat tggaacaaag
     8641 gaacagaaac ggtgacaaaa aaaatgccga actgctactt tatcccaatc aatgatttgc
     8701 tttatcaagg attgaatcag gaagtaggtg tcacagaaga aggaacagat caaacaacta
     8761 gcacgacaga aaatctaaat ggcatcaaaa ataatgtgct ctatgaagaa gatcatttcc
     8821 atccaaataa tttaggttat caattgatgg caaatgcagt gagagatcag ctgatcgaga
     8881 cacaaaacca atggttagag aaggaggatg atcaataaac atgaaaacaa gatctgacgt
     8941 atcttcaaac aaaacaaaga aaacaaatta ctggaaattc gccttcctta tccttcttgg
     9001 ggtagtactt ggttcgaccg tatttttagg cagccgtatt tttgctaatc gggaaccaga
     9061 actcccagaa ataccagctc ttacggaaag acaaggagat cctgtcttaa cgatcaattc
     9121 aaataaagaa aaagtcaatc aaatcatcag ctttttcctt agtgagtatc aaaaagatag
     9181 tgatattgaa tataaatttt atttggaaaa tgaagcacta ctcaatggta ctttcgaagt
     9241 actaggtttt cctatcgatt tctatttata ttttgatccg tatgtgatgg aaaacggaaa
     9301 tgtccaacta aaagctagaa gtctttctat cggaacattg agtttgccaa tcaaagatgt
     9361 gatgaatatg atcaagagaa actataaatt accagattgg atcgaagtca atacagatga
     9421 tctgactatc atgcttaggt tggatcaatt ccgaatgcaa aacgggatgt atattaaagc
     9481 agataaaatc gacttagtaa atgatgacat tcggtttagt ctatatttgc caaaagatac
     9541 ctctgatgct tcaaacaact caaataccaa caaaactagc accagcagta ctgaaagcaa
     9601 gacaaaagaa aataaaaaat catcggatga gtagtattag agggggaatc ataatggaac
     9661 gagcaatatt tgcaggaggc tgcttctggt gtatgatcca gcctttcgac acgcttcctg
     9721 gtatccatac agtgatgtca ggatatactg gagggcatgt accagatccg acttatgaac
     9781 aagtaaaaag ccaaacgact ggacatacag aagcagtgga gattatctat gatccaacat
     9841 tgatttcata tgaacaatta gtagaacttt actggcagca aactgatcca actgacgctt
     9901 tcggacaatt cgaggatcgc ggtgacaatt atcgcccggt gatcttttat ttgacagaag
     9961 aacagcggat cattgctgaa aaaagtaaga aacagttagc tgaaagtgga cgtttcgaac
    10021 agccaatcgt tacaaaaatc gaaccagccc aaccatttta tctagcagaa gaagaccatc
    10081 aagagtatta taaaaagcat cctgaaaatt ttaaaagaaa ccatgcacgc cgggctgcct
    10141 ttatcgaaga aaattggagg aacgaagggt gaatcgaagc ttttttcatt atttaatgac
    10201 actgcgaggc ggtggcacac atgatgcttt atctgttttc gctacagaag ccggtaaaga
    10261 catccaattt cctaagcaca gctctagcta cgaagaaatt tctaattatc tggaactgaa
    10321 tgtcgactat ttgccgtcca tggatatctt tgacgaagca tgggaaaaat atttagaaaa
    10381 taatcatgac atataagaga tagttttgta ctttatgatc aaaaaagttt tttgtccaat
    10441 agcctaattg aaagaattat ttttatttca gctatctagt cgcatttttt tgatattttt
    10501 cttataataa agaaagaatg aaaaaaagag ggatggattg atagagattg acaaaaaaac
    10561 aacgctatat taatcgcttc tggcttgtat tcaaatactt acttgtttct gtattccttg
    10621 cgttcatgtt gcggggattt ttattcatcc cggtaccagt ggaaggcaat tcaatggaaa
    10681 acgtgttgaa gcaaggtgat atggttgtga tggaaaaatt ttcggagatc agacgatttg
    10741 atattgttgt ttttcaatta gcagatggca cgatttatat caaacgagta atcggtcttc
    10801 caggagaaaa tgtcagctac caaaatgatc aattgaagat caatgggaaa gtagttaagg
    10861 aaccttatct aactaaaaac ctgaaatcag atcatgcaaa tgcatcctat acgacggact
    10921 ttacactaca agagctaacg ggacaatcca agcttccgga agataattat tttgtacttg
    10981 gagataatcg aagagtttca aaagatagtc gttcgtttgg cacgatcaat aaaactgata
    11041 ttctaggtaa agcaagattt gtctattatc cacttgatga aataaaatgg atccagtgag
    11101 aaacaataaa gcgtgcagtc tgttgagact gcacgcttta ttgtttaatc caaaatcagt
    11161 ttactacctt agtacgtaaa atcaaacagc tcgtttaaac acttttctag cctctaaagt
    11221 tttgataaat gaataaacaa ccattatttg aatagggatt tggatcatat tcctgatgaa
    11281 tctacctaga aaaattactt tgaaatctgc accaaacaaa acagacatca gccaagaatt
    11341 gataaaaagg ctgactggga tcataatcaa taattgggca gcgatgacgg aaggcaggtt
    11401 tatcttttta tgatataaaa ataagccatt aataaatcca ccaaggaaag ccgtgataag
    11461 aaaagcagct tgaaacgttc ctgaaccaaa tatcgtgaaa tttaaagtgt tcgacaacat
    11521 ttcaagcaaa ccagcccaga tcggtccata tagataaccc ataagcgcca tacttaaaaa
    11581 tccgaatgtg atttgaaaat aagcaggacc aatcgctatc ttgtataaga tgacatcaag
    11641 ggctaataaa agagcagagg tcgacaacaa tcttgcattc atttcgggca atctaaaaac
    11701 gtcttttgca atcaaagtgt tcaaaatatt ctctcctttg tcagttaata ataaaaggat
    11761 accataaaat caccaatgtg gcatgacaca ttggtgattt acatacactg gcatctaaca
    11821 attaaaaaaa gggtgagaag actgctttta taaactgtaa aaaagagatt gtacgaataa
    11881 aaaaaataga ggcggagaat acttatcgta tcatccgcct ttgttttttt gttataaaaa
    11941 acgtcagatt agttatctgc ttacatcttc atccatacgg gttctaatgt atttccgtct
    12001 agatcttgga cttctaatga aaacatttct tcttcaggca ttcccatatc tacttcgtgg
    12061 aaagtaccac cattttcttt tgcaatttga gcaaattttt ttacttcatc cgcgttttca
    12121 acactaaacg aaatcaaagc accacttgtt tttttattat cagctatttc tttatctttt
    12181 ataaattttt tataaaaatc atgagagaga agcattatcc aaaaagtgtc atcccatacc
    12241 attgaaccag cttcttcagt agaaaattct tcattttttt taaaacccag cttttcataa
    12301 aaagcaacgg aacgattgac atcagcaaca ggaaaattaa caaaaaccat actattcatt
    12361 tgtttcgtct cctttctgtt ttgttcgtct atcatctctt atttaggaaa cgctgtcaaa
    12421 aatttagatt catatataga aaccaaaata aaaatttgca tcatcaaaaa agtgaagatg
    12481 tgatagaatg aggactggtt cactttcact taactaatag atggaatttc tttataataa
    12541 aaaagtgaat ttatattgaa aaagaaaaaa acaagtaaag ccggggaata ttccggtatg
    12601 aaaggaatgg agagaacaat gaccatccaa tggtttccgg gacatatggc aaaagcccga
    12661 cgagaagttt ctgaaaaaat gaaatatgtg gacatcgtat ttgaattggt agacgcacga
    12721 ttacctttat cttctcgtaa tccaatgcta gatcagatca ttcagcaaaa acctcgcctt
    12781 gttttattga ataaagcgga tctggcagat ccacaacaga caaagctgtg gcaagaatat
    12841 tttcgtaaac aaggccatct tgcattagct atcacggcac aagaaagtaa aggaattaaa
    12901 ggtctagtac ctgcggctaa agaagcattg aaagaaaaac gggcaagaga tgctgctcgg
    12961 gggatcaagc ctcgcgctat tcgggcaatg gtcttaggta ttccaaatgt tgggaaatct
    13021 acgttgatga atcgactagt aggcaagaaa attgcacaga caggaaataa gcctggcgta
    13081 acaaaaggtc agcagtggct tcgtttaggt aatgagttgg aattgttaga cacaccaggg
    13141 attttatggc ccaaatttga agatgaagaa atcggcaaaa aactagcgct gacaggcgcg
    13201 atcaaagacc agcttgtcca tttagatgat ttagcgattt acggattaga cttttttgcc
    13261 cgatattatt caggaagaat cagcgatcgt tatggtttat caatcgagca agaagaaaaa
    13321 ttggcaccgg aattactgat ggatattaca gcaaaacgtg ggtataaaga agactatgat
    13381 cgtggcagcg aattagtcgt gcatgatatt cgtcaaggaa aacttggacg atacacactg
    13441 gatcgttgcg aagaaatgga gcaaaaaagt gatgactgaa tccatccaaa gtatcaaaga
    13501 acgattgaaa agcatacaga gaatagatga tcctacacta gaatcattaa gaaatgatcc
    13561 acgcaaaggc gtgcagcaag cactggcttc ttttgaacga cgattgaaaa aggaagcatt
    13621 aattaaggaa aaatatgaag aaatgttttg ctttgaaaag caagggtacg cacaaggtca
    13681 ccaatggatt gccggcattg atgaagtagg aagaggacca ttagctggac ctgtcgtggc
    13741 agctgcagtt atcttgccga aaaatgagat catattaggg ctgaatgact ccaaacaatt
    13801 atcagagaaa aagcgagaac ttctttatca acaagttcaa gaaaaagccc tggcaatcgg
    13861 gatcgggatc gttgatcagc agacaatcga tgatgtgaat atttatgaag caagtaaatt
    13921 agcgatgatc caagcagtag aaaaattgtc cattgtacca agctatttgt tgatcgatgc
    13981 gatgaccttg gatttgccta ttcagcagga aaaaataatc aaaggggatg cacgatctgt
    14041 gtcgattgct gctgcaagta tcattgctaa agtatatcga gaccaattga tgaaagaata
    14101 tgacgtgctt tatcctggat atggtttctc gcataatgca ggttatggaa caaaggaaca
    14161 cttagaagga cttgaacgac aaggaattac gccaatccat cgtaaaagtt ttgcacctgt
    14221 aaaaaaatat ttacaataaa aaacagccgc ttttacgtat cttattagaa agagagacga
    14281 aaggcggttt tatgatgtat caaatagaag aaaatttatt gaaacttgct tatacaaaag
    14341 gaatcagtag tcaaggaaaa tgggcacttg ctaactggat gatgcagtat ccttataaag
    14401 aaatcggatt tgaagacatc attaagatag gtaaaataac caaacatcgg gagctgtttc
    14461 gcaaaagttg gcaggaaatt cacgctaact ggggcaaaat ccaatcgaaa cagtcgttta
    14521 tcacttgctt tgatcccaaa tatccccctc agctgcttca cttgacctat ccgccaattg
    14581 tactgtttta taatggtgat ctatctttac tttcttgtaa catgttaagc gttgttggtg
    14641 gaagacacac ttctgcttta gccaaaaaaa cagtttatta tttactaaaa ccagtgatag
    14701 aagctggata tgtgatcgtc agtggctgcg cgaaagggat agatacatac ggtcaccaag
    14761 cagcaatctc tcatggtgga cggacaatcg cagtcattgg aacagggata gagcaagtct
    14821 atcctgctga aaatcgtgac ttgcaagagg tgattgcaaa tgatcatctt cttttgagtg
    14881 aatatcctcc tcaatccggc ccgaaaaagc attgttttcc aatgagaaat cggattatcg
    14941 cagctttgtc taaaggaaca tgtgtctttg aagccaagaa aaaaagcggc tctctgataa
    15001 cagcacagca agcattggat cttggaagaa cagttttttc tgttccagga aatattttaa
    15061 cagaacattc taccggatgt catcatttga tccaagacgg tgcagtttgc acggtttcgg
    15121 gtgaagatat cttggcagaa tttaaagaat aacaccatac ttttcatttg cttttcttga
    15181 caaacgtttt ctgaatggat atgattagct acgatttgta tgaatcctct tttaatagta
    15241 ggaacacgac aatggaaagg agtccattca gtaatggcgt ataaatattt agtgattgta
    15301 gaatcacctg caaaagccaa gacaattgag aaatatcttg gacgaaacta taaagtaatg
    15361 gccagtgttg gccatataag agatttaccg aaaagtaaaa tgggtatcga ttttgaaaac
    15421 aattatgaac cccattatat ttctatacgc ggaaaaggcg atgtcatcaa aagcctgaaa
    15481 gccgcagcaa aaaaagctca aaaagtttac ttggcaagtg acccggatag agaaggagaa
    15541 gcgattgctt ggcatttagc gtacctactt gggttggatc tgaaagaaaa aaatcgggtg
    15601 gtcttcaatg aaatcacaaa agacgcagtc aaagcagctt ttaaggaacc aagaacgatc
    15661 gatgtagatt tagtagatgc acagcaagct cgtcgtacct tagacagaat cgttggttat
    15721 tcgatcagtc ctattctctg gcgtaaggtc aagaaagggt taagtgcagg acgtgtccaa
    15781 tctgtcgctt taaaaattat tattgaccgt gaaaaagaga tccgagaatt tgttccagaa
    15841 gaatattgga gcatcgacgg taattttaaa aaagctcgca agaaattcaa agcaaatttc
    15901 tggggaatcg acggtaagaa aaagaaatta ccagatgcac aaagtgtaaa agaagtcact
    15961 gctagaattg atggaacaga cttcgaagtt aaaaaagtag aaaagaaaga gcgcaaaaga
    16021 aaccctgcga atcctttcac tacaagtagc ctgcaacaag aagctgcccg taagttaaac
    16081 tttcgaacaa gaaaaaccat gatggtagca cagcaattat atgaaggaat cactttgggt
    16141 aaacaaggaa cagtcggctt gattacatac atgcgtactg attctacccg gattgccgat
    16201 acagcaaaag cagaggccgc agcatttatt gaagatacgt atggaaaaga attttctaga
    16261 catgatcacc gaaagatcaa aaatgctcaa ggcgcacaag atgcccatga agctgtgcgt
    16321 ccaacgagtg tgcttcgtac accagatgaa ctcaaacaat atttagataa agatcaattg
    16381 aaactttata cattgatctg gtcacgtttt gtggctagtc agatgacacc agctatttta
    16441 gatacaatga aagtgacatt agaacaaaat ggtgtcatct tcattgcgaa cggttcaaaa
    16501 atcaaattca aaggattcat gcaagtgtat gtggaaggta gagatgacgg caaagaagag
    16561 aaagaaaata ttcttccaga acttgaagaa ggcgatgtag tccaatctgt tgatattgaa
    16621 ccaaaacaac attttactca accaccagca cgttttagtg aggctacgtt gattcgtgca
    16681 ctagaagaaa acggggtagg gcgaccttcc acttatgcac caacacttga tacgatccaa
    16741 agacgctact atgtaaaatt gacacaaaaa agatttgagc cgactgaact aggagaaatt
    16801 gtcaattcat taatctgtga attcttccct caaatcgtcg atatccattt cacagcagaa
    16861 atggaaggag atttggataa aattgaagaa ggcacggaag cgtgggtcaa agtggttgat
    16921 cgtttctata aaccgtttga aaaagaactg acgaatgcgg aagaaaaaat cgaaaaaatt
    16981 caaatcaaag atgaaccagc aggattcgat tgtgatgtct gtggacatcc gatggtgata
    17041 aaattaggga aatatgggaa gttttatgct tgcagtaatt ttcctgattg tcggaatacg
    17101 aaaccaatcg taaaagaaat cggtgtgact tgcccagtat gtcatgaagg acaagtcatt
    17161 gaacgaaaat ctaagaaaaa cagactattc tatggttgtt cccgtttccc agaatgtgaa
    17221 ttcacttctt gggataaacc agtgggcaga aattgtccaa aatgtgacgg atatctggtc
    17281 gagaagaaag tcaaaggcgg taaacaagta gtctgtgtaa atggagatta cgaagaagac
    17341 atccaaaaat aatagaaaag aggttctttc attgaaaatg gtaaatgttg ttggagcagg
    17401 gcttgccgga agtgaagctg cttggcagat cgcacaagca ggagtccctg tccgtttata
    17461 tgaaatgcgt ccagtcaaat cgactgaggc gcatcacaca agtaattttg ctgaactagt
    17521 atgttccaat tcattaagag gaaacagttt ggctaatgca gtagggttat tgaaagaaga
    17581 aatgcgccga ttgaattcgg tagtaatcac ttcggcagat gaaacagcag tgccagctgg
    17641 tggtgcctta gctgttgacc gcgattcttt ttctgaaaca attacccgaa aagtcaaaga
    17701 gcatccatta gtaacagtga taaacgaaga aattactgaa ataccggaag gaatcaccgt
    17761 agtagcaaca ggacctttga cttctcatcc attagcagaa agcatcaaag cattcaatgg
    17821 ttcagatggt ttttatttct atgatgcagc tgcaccaatc gtagacaaag ctacgatcga
    17881 tatggataaa gtatatttga agtcccgcta tgacaaagga gaagcagctt acttgaactg
    17941 tccgatgaca gaagaggaat tcaaagcttt ccatgaggca ctaatcaatg ccgaagtggc
    18001 agaacttaaa tcttttgaga aagaaaaata ttttgaagga tgtatgccaa ttgaagtaat
    18061 ggcacagcga ggattcaaaa cgatgctttt cggacctatg aaaccagttg gattggaaga
    18121 tcctaaaact ggcaaacgtc cttatgctgt tatccagctg cgtcaagaca atgctgcagc
    18181 ttctttatac aacatcgtgg gctttcagac tcacttgaaa tggggcgagc aaaaacgtgt
    18241 tttccggatg attcctgggt tagaaaatgc tgaattcgtt cgctatggtg tgatgcaccg
    18301 aaacagcttt atgaattcgc cagaattgct gaaaccgact taccaatcaa cgaaacgcga
    18361 agatctgttc tttgcaggtc agatgactgg tgtagaaggc tatgttgaaa gtgcagcgag
    18421 cggactactt gctggaatca acgccgcacg cttagcaaaa gaaatggaac cagtcgagtt
    18481 tccacaagaa acagctattg gaagtatggc ttactacatt actcatgcgg aaggaaaaca
    18541 tttccaaccg atgaatgcca actatggtct gtttcctgaa ctgccagagc gcattcgaga
    18601 caaaaaagca agatatgaag caattgcaca acgagcttta aaagctaatg aggcaataaa
    18661 aacagaatta gaaaaaactg caatcccaaa tgagtaagga aactccatga agagggtgag
    18721 acggatagtc tcgctctttt tctggcgttt ttcttaagaa tttctcaaac aaaatgtgag
    18781 gaattgcgcc aattgtgaac aaacttagaa atattggtta aattctgaca atttatttgt
    18841 ctttttataa acgctgtgct aaattattct ctgggacaaa aggaaggtga gaaaatgaca
    18901 gaagccacgt gggaacaaaa atttttgcgt tatttgattg tcgaaagagg atattcagaa
    18961 aaaacgaaag aagcatatga agaagacatt ctccatttca aacatttttt ggaggaatct
    19021 ggagacgctg atctgctgaa ggcggaatat tttacggttc gtgtctattt gagtgcatta
    19081 gctgatcatc gtcctagcta tagcagaaat tctatcagtc gcaagatagc cagcctacgt
    19141 tcgttttatc agtatcttct caaagaagaa gtgataaaag aaaatccatt ttcttacgtt
    19201 catctcaaaa agaaaaattt gagactgcca cgtttttttt atgaaaatga aatgcaagca
    19261 ctttttgaca gcgtagcagg agatagtcct ttggatttga ggaaccgcgc tttactggaa
    19321 gtcctttacg ggtcaggcat ccgtttaagt gagtgtagca gtttgtcggt atcagacatt
    19381 gattttgatt cagaggtcat gttgatccat ggtaaaggga acaaagagag atatgctcct
    19441 ctaggttctt ttgctcaaga tgctctgcaa gaatatttca gggaaggccg ccaggtattg
    19501 atgacaaaat atcacgaaga gcatgatatc gtttttgtga atcatcatgg aaaaccaatc
    19561 acgcctactg gaattgaata tgtcttgaat caaatgatca aaaaaagcag tttggatagt
    19621 gatatccatc ctcacatgct gcgccataca tttgctactc atttattaaa taatggggca
    19681 gatttgcgga cagtccaaga attgctagga catgccaatt tatcgactac ccagatctat
    19741 gcccatgtaa caaaagaaag tttacaaaaa aactatcgtt ctttccatcc aagagcgtaa
    19801 aaataaggaa aagaacaa
//
LOCUS       DACECZ010000041        19480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.041, whole genome shotgun sequence.
ACCESSION   DACECZ010000041 DACECZ010000000
VERSION     DACECZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.041"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            133..3660
                     /locus_tag="IWO35_001384"
     CDS             133..3660
                     /locus_tag="IWO35_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0316446.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IWO35_001385"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IWO35_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ0316447.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IWO35_001386"
     CDS             7448..7789
                     /locus_tag="IWO35_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0316448.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IWO35_001387"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IWO35_001387"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0316449.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IWO35_001388"
     CDS             9146..11566
                     /locus_tag="IWO35_001388"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ0316450.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IWO35_001389"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IWO35_001389"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ0316451.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IWO35_001390"
     CDS             13000..13737
                     /locus_tag="IWO35_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0316452.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IWO35_001391"
     CDS             13751..14566
                     /locus_tag="IWO35_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0316453.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IWO35_001392"
     CDS             14579..15226
                     /locus_tag="IWO35_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0316454.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYLEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IWO35_001393"
     CDS             15242..15898
                     /locus_tag="IWO35_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0316455.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IWO35_001394"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IWO35_001394"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ0316456.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IWO35_001395"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IWO35_001395"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ0316457.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IWO35_001396"
     CDS             18240..18758
                     /locus_tag="IWO35_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ0316458.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IWO35_001397"
     CDS             18865..19365
                     /locus_tag="IWO35_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0316459.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6552 a         3343 c         4182 g         5403 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctatttag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag
//
LOCUS       DACECZ010000042        19405 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.042, whole genome shotgun sequence.
ACCESSION   DACECZ010000042 DACECZ010000000
VERSION     DACECZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.042"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            2..550
                     /gene="hslV"
                     /locus_tag="IWO35_001398"
     CDS             2..550
                     /gene="hslV"
                     /locus_tag="IWO35_001398"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ0316460.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            564..1964
                     /gene="hslU"
                     /locus_tag="IWO35_001399"
     CDS             564..1964
                     /gene="hslU"
                     /locus_tag="IWO35_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ0316461.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1982..2773
                     /gene="codY"
                     /locus_tag="IWO35_001400"
     CDS             1982..2773
                     /gene="codY"
                     /locus_tag="IWO35_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ0316462.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2855..3730
                     /locus_tag="IWO35_001401"
     CDS             2855..3730
                     /locus_tag="IWO35_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ0316463.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3862..4500)
                     /gene="plsY"
                     /locus_tag="IWO35_001402"
     CDS             complement(3862..4500)
                     /gene="plsY"
                     /locus_tag="IWO35_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ0316464.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4627..5061
                     /locus_tag="IWO35_001403"
     CDS             4627..5061
                     /locus_tag="IWO35_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ0316465.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5227..7272
                     /gene="parE"
                     /locus_tag="IWO35_001404"
     CDS             5227..7272
                     /gene="parE"
                     /locus_tag="IWO35_001404"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ0316466.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7284..9734
                     /gene="parC"
                     /locus_tag="IWO35_001405"
     CDS             7284..9734
                     /gene="parC"
                     /locus_tag="IWO35_001405"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ0316467.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10011..12242
                     /gene="pflB"
                     /locus_tag="IWO35_001406"
     CDS             10011..12242
                     /gene="pflB"
                     /locus_tag="IWO35_001406"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ0316468.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12486..13247
                     /gene="pflA"
                     /locus_tag="IWO35_001407"
     CDS             12486..13247
                     /gene="pflA"
                     /locus_tag="IWO35_001407"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ0316469.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13332..14258
                     /locus_tag="IWO35_001408"
     CDS             13332..14258
                     /locus_tag="IWO35_001408"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ0316470.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14440..15333
                     /locus_tag="IWO35_001409"
     CDS             14440..15333
                     /locus_tag="IWO35_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ0316471.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15467..15592
                     /locus_tag="IWO35_001410"
     CDS             15467..15592
                     /locus_tag="IWO35_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0316472.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15726..16358)
                     /locus_tag="IWO35_001411"
     CDS             complement(15726..16358)
                     /locus_tag="IWO35_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316473.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16506..17048)
                     /locus_tag="IWO35_001412"
     CDS             complement(16506..17048)
                     /locus_tag="IWO35_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ0316474.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17183..17572
                     /locus_tag="IWO35_001413"
     CDS             17183..17572
                     /locus_tag="IWO35_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316475.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17576..18340
                     /locus_tag="IWO35_001414"
     CDS             17576..18340
                     /locus_tag="IWO35_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0316476.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18469..>19405
                     /locus_tag="IWO35_001415"
     CDS             18469..>19405
                     /locus_tag="IWO35_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0316477.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRE"
BASE COUNT         6793 a         3292 c         4113 g         5207 t
ORIGIN      
        1 catggtcgaa tcaccatttc attcaaccac tatctgtgcc gtcgaaaaag acgggaaatt
       61 tgctatggct ggagatggcc aagtaacaat gggtgaatct gttgtcatga aaggcactgc
      121 aaagaaagta cgacgtatct acaatggaga agttgttgta ggtttcgccg gaagtgttgc
      181 agatgcgttt actttagaag aaaaatttga aggcaaacta aacgaatata acgggaatct
      241 acaacgcgca gctgtcgaat tagctcaaga atggcggaca cagcaatcta tgcaaaaact
      301 tgaagcgatg ctgatcgtaa tgaatgataa agaaatgctt cttgtttcag gtacaggaga
      361 agttatcaca ccagatgatg gtatcttagc tatcggatca ggagggaatt ttgcgttggc
      421 tgcagcccga gcaatgaaaa actatgctca aaacgacatg agtgcgaaag aaattgcaga
      481 aaatgcgtta aatattgccg cagatatctg tatctttact aatcataata ttatcgtaga
      541 agaactgtaa gaggtgagta gatatgtatg aattaaaaca aacaccaaga caaatcgtaa
      601 gtgaattaga tgaatatatc gtaggacagc acgcagcaaa aaaatctgtt gccgttgctt
      661 tgcgtaaccg ctatcgccgt ttgcagttag atgaaaaaat gcaacaagat attacaccaa
      721 aaaatatgct gatgatcgga cctacagggg tcggtaaaac agaaatcgca cgacgtttgg
      781 caaaaatcgt caatgcacca tttgtcaaag tcgaagcaac aaaatttact gaagtcggtt
      841 atgtcggaag agatgtagaa tcaatggttc gggatttagt agaaaatgcg atccaaatcg
      901 tagaaaaaga gcagtacagc cgtgtataca caagtgctaa aaaaaatgca gaaaaacgtt
      961 tggtaaaaat cttggtacct ggtatcaaaa aagaacagaa acaatccact aatcaatttg
     1021 aacaaatgat gaatcttttt aatgctagtc agcaaaatac cgctgaaccg caagaagaag
     1081 tgacagaaga tattcgaatc aatcgccgta cagtcttgga acaactacaa aagggtttac
     1141 tggatagtcg tgaagtaacc attgaagttg aagaccaaaa gaaacctgca ccaatgatga
     1201 acaatggatt ggagcaaatg gggatcgacc taaatgaaac tctaggtgct ttgacaccga
     1261 agaaaaaagt ggaacggacg gtaacagtca aagaagcttt agagttattg atcaaggaag
     1321 aatcagcaaa attagtcaag gatgctgata tccatagtga ggcaatcaaa ttagcggaag
     1381 aaagtggaat tatttttatc gatgaaatcg ataagatcac ttctaaatca cagcaatctg
     1441 gagaagtatc cagagaaggc gtccaaagag acatattgcc aattgtcgaa ggttcacaag
     1501 taaacacaaa atacgggatc atccaaacag atcacgtcct attcattgct tcaggagctt
     1561 tccatttgtc taaaccaagt gatctaattc cagaacttca aggacgcttc ccgatacggg
     1621 tagaattaga tgatttgaca gctgaagatt ttgtgaagat tctgaccgaa ccaaataatg
     1681 ctttagtaaa acaatacgtc gcgttgatcg gtacagaaaa tgtgtcagtt acgtttacaa
     1741 aagaagcaat tgaacggatc gctcaaatcg ctttcgatgt caaccgtgac acagataata
     1801 tcggcgcacg cagactgcat accattttgg aaaaactact agaagatcta ttatttgaag
     1861 caccagatat gcaaatggga gaaataacga tcacagaagc atatgtggat gacaaacttg
     1921 gctcaattgc ggcaaatgaa gatttaagtc gctatatttt ataaaaaatc ggaggacgac
     1981 aatgacagac ttgctaacga aaacccgcaa gataaacaca cttttgcagc aaaagaatac
     2041 ttttgacatg caagcagatt tgccttacga taaaatggct gtcacactag gtggtattct
     2101 tgacagcaac acctatatca taggaagtga cggagtatta ctaggttata atgaacgtca
     2161 cgatgtgaac aatgaacgaa tcaaaacaat gtttgttgag aaacaatttc ctgaatctta
     2221 tactgataca gtggcacagt tgatgaaaac tgaagctaat atcccaatta ctagtgatat
     2281 tactgcattt ccggtagagt caagagagaa atatccattt ggtctgacga ctgtcgttcc
     2341 gatctttgga gctggcgaac gtttgggtac gattatctta tctcgtatgg aacaatcgtt
     2401 tgcagatgac gatctagtct tagcagaata tggagctacg gttgtcggta tgcagatcct
     2461 ttatcagaag tctcgaagca tcgaagcaga tgtacggagt gcaacggctg tccaaatggc
     2521 aatcaacaca ttatcttata gcgaattgaa ggctgtacaa gctatctttg aagcattgga
     2581 tggagatgaa ggtcgtctga ctgcttctaa tattgccgat agtatcggca tcactcgttc
     2641 tgtgatcgtc aatgctttac gaaaattgga atcagctgga atcattgaat ctcgttcgtt
     2701 aggcatgaag ggaacttatt taaaagtatt gaattcccgt tttaaagacg agttagcaaa
     2761 acacagttat tagaagtaag gggactgggg cagatagaaa tgtttgttcc agccttccca
     2821 ttttaaaaag taagtcgaag ttggaggaat ggcaatgact gttattattc aaaatgacca
     2881 gctgatcgcc gaaatcgctg aacacggtgc ggaattgatc agtctgaaat caaaagaaca
     2941 tggtttagag tatatctggc aaggtgatcc agcgtattgg ggcagacatg caccggtttt
     3001 attccctttt gttggacgtt taaaagatga tcagtatgtt tatcaaggga aaacatatca
     3061 aatggggcaa cacggatttg ctcgggatat ggattttgaa gtcattgaac atggacaaga
     3121 aatggctagt ttcatcttaa ggagtacgcc ggagacatta gaaaagtatc catttgattt
     3181 tgaattagtg atcagctatg aattaggagg agacggcatc acggttcatt atcaagtggc
     3241 aaataccggt gaaaaagaaa tgttcttttc tatcggtgga catcctgctt tcaatgtacc
     3301 tcttgaagaa ggattgtcat ttgaagattt ctatctggca ttttctccat tgaaatcaag
     3361 attacgccta ccattgaaag gcccattcat cgatatggat caaaagacgt taggtcagac
     3421 aaatacgaat ctggctttga atcgtgaact ttttgagcaa gatgccttgg tttttgaaac
     3481 aaggggtcta aatgcgtacg ccattcgaag tgaaaaatct tcccatagcg taactttaag
     3541 ctataaagat atgccttatg tagggatctg gtctccttat ccaaaagaag cgcctttcgt
     3601 ttgtatcgaa ccgtggtgcg gaatcgctga cacggtgaac agtagtggaa acttgatgga
     3661 aaaagtaggg atcaatcaac taggtgccca tgaaatcttc aagacaaaat attccatcac
     3721 agtgaagtaa gaggttgtga ccgagaagtt agcagggaac accgtttacc aaggtgtgta
     3781 aaaaagctat cctgtaagta ataagggagt gtgacatcga tttgtcacac tcccgtttta
     3841 tctctttgga atgaaaaagt atcaggattt tttcttgctg ctattcaaac caaacgggat
     3901 tcgactttca gtatggtttc tgatacgctt gatattgtct ttatgccgat aaaagataaa
     3961 ggaagaaact aaaaaggcaa tgatcgtcag taaccaattg ggcttagcca aaatcacagg
     4021 agctacataa ggaaggacga tcgtagataa agtgatcaat actgcactga tcatactgct
     4081 caaactcacc atacttgtac aaaataaact aacgataaag atgatacaag aataaacaaa
     4141 aaatacagga ttaaatccta aaagcattcc tgcacttgtc gcaacagctt ttcctccttt
     4201 gaacccagca aaaatcggaa atgtatggcc aagaatggcg cataccccaa accaaagagg
     4261 gttaatccca gaaaggtgga agatcatcgg caaacaagta gccaatgtac cttttaaaat
     4321 atccataagc aggacaacaa tcccggctgt tttgcctaat acacgaaatg tattggtcgt
     4381 tccagtatta ccgctcccgt gttgtcggat atcttttttg aaaaacaatt tgccaatcca
     4441 gacaccggaa ggaatagaac ctaaaagata tgctaaaata agtaataaga tcaatttcat
     4501 tttttaccct ctttcaagtt gaaaactatc ctttatttta gcatgaatag gtaacaggaa
     4561 caattgaaaa aatgaaagat acaagtataa tagatggaat aaaaagaaaa gcaaggggtg
     4621 gcagaaatgt ctgtaaaaaa tccaacacaa gaaacgattt tcaactattt ggaagaagca
     4681 aaaaatattg ccgtcgtagg cttgagcaat aaaccagaac gaacaagtta catggttgca
     4741 caggtattgc aagacaatgg atataaaatc ataccagtaa atccattatt ggctggtgaa
     4801 gaaattctag gcgagaaagt ctatgcgaat ttgacagaaa tcgatgatcc aatcgatatc
     4861 gtagatgtat ttagacggag tgaattttta cctgaaatag caggtgattt tttgaaaact
     4921 gatgctaaag tgttctgggc acaattaggg attgaaaaca aagaagcagc tgagatgcta
     4981 agaaaagcag gacgtacaga tatcgtaatg gatcgttgta ttaagattga acttggtaag
     5041 ttgaaagaaa accaaaaata aaaatgacca caaagaagag atgatcgata gaggggaatg
     5101 tcgaaaaagc ggttaggaga taattaaaat tgatttctcg tctaccctct tttttttcac
     5161 gaccttttct agtatgataa tcgtggtgcg gttttgccgc aaagtattat tgaaggagcg
     5221 tttgccttgg caaaaaaagt aaacaacgaa tacaatgact cgtccatcca agtattggaa
     5281 ggattagagg cagttagaaa aagaccaggg atgtacattg gatccactga cagccgaggc
     5341 ttgcatcatt tagtctatga aatcgtcgat aatgcagttg acgaagcatt atcaggttat
     5401 ggcagtgaga ttgatgtgac catccatgaa gacaatagta taactgttgc agatagcgga
     5461 cgtgggatgc ctgtaggaat gcatgcttcc ggaataccaa cagttgaagt gatttttact
     5521 gtgctgcatg ctggcggaaa gtttggtcaa ggagggtaca aaacttctgg tggactgcac
     5581 ggtgtaggag ccagtgtggt aaatgcctta tcgaaatggc taattgtaac gatcgttaga
     5641 gatggcgtag agtatcagca gaaatttaaa aacggcggta aacctgacgg tactttgaaa
     5701 aagataggaa aaacaaaaaa agcaaacggg acgactgtcc attttttacc agatgataca
     5761 atattcagta caacgaaatt ttcatatgaa attttagccg aacggttaag agaatctgcc
     5821 tttcttctaa aaggcgttaa aatctctctt tctgatcttc gtggagaaga accagtcaag
     5881 gaaatttttc attacgaaga aggaatcaaa gaatttgttg attatctgaa tgaagaaaaa
     5941 gatacattga caccagttgt ttatttttca ggagaaaaag aaggtatcga agtagaggtc
     6001 gcttatcaat acaatgacgg ttattcagaa aatgttttat cttttgtcaa taacgttcgg
     6061 acaaaagatg ggggaaccca tgaagctggt atgaaagcgg cgatgacgaa atcttataat
     6121 gaatatgcga gaaaagttgg tttgctgaaa gaacgggaca agaatctgga aggtagtgat
     6181 ttcagagaag ggttagctgc tgtactttct atccgtgtac ctgaaaatct tctgcaattt
     6241 gaagggcaaa caaaagaaaa acttggtacg ccagttgctc gtaccgtagt agataacgtc
     6301 atcagtgaac aaatgggctt ttatttgcag gaaaatagcg aaatgagcca gatgttggtc
     6361 cgtaaagcaa tcaaagccag agaagcgaga gaagcagcac gtaaagccag agaagaaagc
     6421 cgaaatggga agaagcgtaa aaaaggcgaa tctcttcttt caggaaaatt gacacccgca
     6481 caatcccgaa accctaagaa aaatgaattg tatttagtcg aaggggattc tgctggcgga
     6541 tctgctaaac aaggaagaga cagaaagttc caagcaattc tacctttacg aggaaaagtc
     6601 atcaataccg aaaaagctaa aatgcaagat atcttgaaaa atgaagaaat caatacgatg
     6661 atctatacga ttggtgcagg agtcggtcct gagttttcta tcgaagactg taattacgac
     6721 aaagtaatca tcatgaccga tgcggataca gacggcgccc atattcaagt attactgctt
     6781 actttctttt atcgttatat gaaaccgctg attgaagctg ggaaagtata tatcgcgtta
     6841 ccgcctttat ataaagtatc caaaggacaa ggaaagaagc aagtcattga atatgcgtgg
     6901 acagatgacg aattggcagc aatgattaaa aaagtcggca aaggatatat gctgcagcga
     6961 tataaaggac ttggagagat gaatgcggaa caattgtggg aaacaacgat ggatccgact
     7021 tcccgtacgt tgatccgcgt ccgtatcgat gatgctgcgc aagctgaacg tcgagtaacc
     7081 acactgatgg gtgacaaagt agaacctcga agaaaatgga tcgaaaatca cgtgcaattt
     7141 acgctcgaag aagatggcag tatcttagat aaaaaagagg atactgaaat ctctccttca
     7201 gtttctaatg atttattaga tgaagaacga gcagataaaa atgaaaacaa tcagttattt
     7261 gaagtagaat aggaggtcac ctgatggaaa atcgacaaga agtacaagaa ttaactttag
     7321 aagaagtgat gggtgaccgc ttcggacgtt attccaaata tattatccaa gaacgtgcac
     7381 tgcctgatat ccgtgatgga ttgaagccag tgcaacgccg gatattattt gccatgaaca
     7441 aagatggcaa tacgtatgac aaaggattcc gtaaatcagc aaaatctgtc ggaaatatca
     7501 tggggaatta tcatcctcat ggtgacatca gtatttatga agcgatggtc cgcatgagcc
     7561 aagactggaa gttgcgggaa gtactgatcg aaatgcacgg gaataacggg agtatggacg
     7621 gtgatccacc tgctgcgatg cgttatactg aagccagatt atccaagtta agtggagaga
     7681 tgctagcaga tatcgagaaa gaaacagtcg atctcgtgtg gaattttgat gatacagaaa
     7741 aagaaccaac agtccttcca gctcgatacc caaaccttct cgtcaatgga tcgacaggta
     7801 tatctgctgg atatgcaaca gaaatcccga ctcataactt agctgaagtg atcgatggca
     7861 caatctatat gatcgatcat ccacaagcga gccttgaaaa attgatggaa tatattcctg
     7921 gacctgactt tccaacagga gggatcttgc aaggaaaaga tgaaatcaaa aaagcttatg
     7981 aaacaggacg aggaaaagtt attcttcgtt cgaaaacaaa gatagaggca atgaagggta
     8041 ataaacagca aatcgtgatc acagaaatcc catatgaagt aaataaagcg accttggtga
     8101 aaaaaatgga tgagatccgt ttgaataaga aaatcgatgg aatagctgaa gtacgagatg
     8161 aaagtgatag aacaggtctg caaattgtcg tcgaattgaa aaaagacgcc aatgctcaag
     8221 gaattttgaa ttatttgttc aaaaacactg agctacagat caattacaat ttcaatatgg
     8281 tcgctatcga ccatatgact ccgcatcaag ttggactgaa agacatttta agaagttata
     8341 tcgagcatcg aaagcaagtc ataacaaaac gtagtcaatt cgatttagcc aaagctcaaa
     8401 aaagacagca tatcgtagaa ggattgatga aagctttatc gattctggat gaagtaatcg
     8461 caacgattcg tgaaagtaaa gacaaaaaag acgccaaaaa gaatttagtc gacgtctttc
     8521 aatttacaga agaacaagca gaagcaattg tcacattgca attataccgt ttgacgaaca
     8581 cggatatcac agagcttcaa aaggaatccg aaagcttgat tgcgcaaatc acagagttga
     8641 ataaaatcct ttcaaatgac aaagagcttt tctctgttat gaaaaaagaa ttgcgagaag
     8701 tgaaaaaaaa ttattcttcc gctcgtctaa cgacaatcga agacgaaatc gaagaaatca
     8761 aaattgatac gcaagtctta gtggcacaag aagacgtaat tgtcagtgtg acgcgagaag
     8821 gctatgtgaa gcgaacaagc cttcgttctt atagtgcttc caaaccagaa gaaatcggga
     8881 tgcgagaagg cgactacttg ctatactcag gtgaattgag tacattggac catgttcttc
     8941 tgatcacaaa taaagcaaat gtgatctacc gccctgtcca tgaactaccc gatttgaaat
     9001 ggaaagatgc tggtgagcac atttctcaaa caatctccaa tctgtctgta gacgaatcga
     9061 ttttagctgt tttcccatat caaaaaattc aagcagagaa aacatttgta ttcatcagca
     9121 agaatggatt gatcaagcaa acacgaatga cagagttcga accatggcgc acctataaga
     9181 gccgtccgct cagctgtatg aagctaaagg cggaagaaga tgaactgatt gctgtgtatc
     9241 tgaaagaaga tcagccaggc ttagatgttt tcttagtaac gcatcaagga atgggtttgc
     9301 gctatccttt aacagatgtt cccgttgttg gagcaaaggc agctggagtg aagtctatga
     9361 atttgaaaga tggagatagg attatcaacg ggctgcttgt gttagcagaa ggtgatacgc
     9421 cgatcgtaat cgttactcag cgaggagctg ttaaacgcat gcttgctcaa gaaatcagcc
     9481 aaacttcacg tgcgaagcgg ggagtgacgg tcatgcgtga gctgaaaaaa aatccgcacc
     9541 gtattatcta tatgtcggaa ggaagatcaa aagaaataac cttgatgaat caaaaagggc
     9601 agcagttaac gattgaccca actgatttcc ctataggtga tcgaacatcg aatggatcat
     9661 ttgcgatgga tgagaaaaaa ggtggagaag tcatcgaagt tatcgatgct cctgagattt
     9721 tcatcgagtc gtaactgttg tagtatttta atgaagagac gccatctgta ttaatacagg
     9781 tggcgtctct ttttcattca tttttaataa atccttatac gtttgcaaaa aaagatgaaa
     9841 gtgttcacaa tgcttaaatg ttggtatgtc aacgttgatt taaatatatg taaataaaac
     9901 ataaaaattg ttaaataaaa aacaagaaaa cccttgcaaa gatagggtgc tgtgttatac
     9961 taatcacgta aaaactgtta cataaaacag aaatttagga ggtccataca atggagcaat
    10021 ggaatggatt taaaggtacc aaatggaaag aaggcgtaga cactagagac tttattcaaa
    10081 ataactacac tgaatatcgc ggtgatgaca gctttttgga aggcccaaca gaagcaacag
    10141 aaaaattatg ggatgaattg caaaagttaa atgatatcca attcgagcgt aacggtgtct
    10201 atgatatgga cacagaaatc gtatcaacta ttacttcaca cgatgcaggc tatttggata
    10261 aagatttgga aaaaatcgtc ggtctacaga cagacaaacc tttgaaacaa gcattcatgc
    10321 cgttcggcgg aatcaatatg gctaacaatg cgctaaaaag caatggctat acaccaaacg
    10381 atgaattgac acatattttc actgactggc gcaaaacaca taatcaaggt gtatttgacg
    10441 cttatactgc tgaaatgcgt gctgctcgta aaaacaaaat cattacaggg cttccagacg
    10501 cttacggtcg cggacgtatc atcggtgact atcgtcgtgt ggcattgtat ggaatcgact
    10561 atttgatgga acaaaagaaa aaagatcatg atatgactgg ttatcataca atgagtgaag
    10621 aagtgattcg tttacgtgaa gaaatcactg aacaatatcg tgcattagcg caaatgaaag
    10681 aaatggctgc gaaatatggc tatgatattt ctaaaccagc tgcaaatgca aaagaagctg
    10741 tccaatggct atacttcgga tatttagcag caatcaaatc acaaaatggt gctgcgatgt
    10801 caatcggacg tatctcagca ttccttgata tctatatcca acgtgatttg gataatggag
    10861 taatcacaga aaaagaagca caagaattga tcgaccactt agtaatgaaa ttacgtatgg
    10921 tcaaattcgc gcgtacacct gaatataacc aattattctc aggattccct atctgggcta
    10981 ctttatccat tgcaggtatg ggattagatg gtcgttcatt agttacaaag aatgatttcc
    11041 gtctgcttca tacattaaca aatatgggac cttcaccaga accaaacttg actgttttgt
    11101 attctgaaca ccttccagaa ggattcagaa catatgctag cagaatcgct atcgaaagtt
    11161 cttcgattca attcgaaaat gataatcagc ttcgtgaaca atggggttct gatgattgtg
    11221 cgattgcttg ctgtgtatca ggaacagttg tcggaaaaga catgcaattc tttggcgctc
    11281 gtgctaactt ggctaaaaca ctattatatg ccatcaatgg cggagtcgat gaagtgactg
    11341 gcgcccaagt tggaccagaa tatcgtccaa tcacatcaga aatactagac tttgaagaag
    11401 tgaaacataa cttcatgaat atgatggatt ggttagcaga actatatgtc aatacgttga
    11461 acatcattca ttatatgcat gataaatatt cttatgaagc agctcagctg gctttgatgg
    11521 attctgactt gcaacgcaca tttgctacag gtattgctgg gatctctcat gcggcagaca
    11581 gcttgtcagc gatcaaatat gcaaaagtaa aagcaatccg taatgaaaaa ggaatagcag
    11641 ttgattttga agtggatggc gacttcccta aatacggaaa tgatgatgac cgtgcagatg
    11701 aattagtttc atggatcttg gaatacttca tgggacaaat caaacgtcat aaaacttacc
    11761 gcaactcaaa accaacaact tcattattga cgattacttc aaatgttgtt tacggtaaaa
    11821 atacaggaaa tactcctgat ggccgtgaag ctggaaaacc acttgcgcca ggagcaaatc
    11881 caagttacaa tgcggaacaa agtgggttat tagctagcct gaactcaaca gctaaaatga
    11941 attacggctg tgcattggat ggaatttcaa atacccaaac aatcaatcct tcaggtcttg
    12001 gaaaagaaca agatacaaga atcgaaaact tgcgtaatgt cttagatgga tacttctcaa
    12061 aaggagctta tcatttgaat gtcaatgtat tcagtaatga tttattgcgt gatgcaatgg
    12121 aaaatccaat gaaatatccg aacttgacga tccgggtatc tggttatgca gttaaattcc
    12181 gtgacttgac aagagaacaa caattagatg tcttgatgag aacttcacac gacagaatgt
    12241 aaaaagttcg tgaaaaaagt ttttcgactt agcgtcgaaa ggacgttgaa tagccatcgc
    12301 atcaaaatcg aataatcagt atgcttattt gaaagaaacg atttacaggg gcaggacttg
    12361 tatgttccgt ccctgtaaat tttacacata acgaagcggc aattgcgaaa aattttgttc
    12421 agaaagcttc gcttcgacag ctaacttgta cttaaaatga aatcaaagaa aagatgtgaa
    12481 cttaaatgga agaaaaaaca atcggctatg ttcattctat cgaaactttc ggttcagtag
    12541 acggacctgg actgcgcttt gtcgtgttta tgcaaggatg ccgcatgcgt tgccaatttt
    12601 gtcacaatcc agatacttgg aatattggcg gagggaaaga atacacagca gatgaacttt
    12661 tagataaagc agaacgcttc agaccttact ggggagataa aggtggtatt actgttagtg
    12721 gtggagaacc tctattgcag atcgattttt tgatcgaact atttaaaaaa gcaaaagagc
    12781 ggaagatgca tactacattg gatacatgcg gaaaaccttt tacgtatgaa gaacctttct
    12841 tttcacgttt tcaagaattg atgaagtata cagatttact gttatttgac atcaaacaca
    12901 tcgataatga agagcataaa aaactaacgc attgggacaa tgaaaatatt ttagaaatgg
    12961 ctcaatattt gtcaaaaatc aacaaaccgg tttggattcg ccatgtacta gttcctgagc
    13021 gaagtgatta tgatgaatat ttgatccgac tagacaactt tattcaaacc ttgtcaaatg
    13081 tagacagagt agagattctt ccttatcata cgatgggaaa atacaagtgg gaaacacttg
    13141 gtttgaaata tccattagaa gggatcgaac cacctacaaa agaacgtgtc gaaaatgcta
    13201 aacgattgct tcatacagaa gagtatacaa aatatttaac gcgataggtc gcatcggaaa
    13261 gaagataagt attgcttctt tccgtttttt tgctattatt aaataggaga aatcacaaag
    13321 gagcgaatga catgtcaaaa attttagttt ttggtcacca aaatccagat acagatgcaa
    13381 tcggggcagc cattgcgttt tcacacttgc aaaatgaatt aggtaaagaa acagaagcag
    13441 ttgctctagg tacacctaat gaagaaacac aatacgcatt agaccacttt cagctatctg
    13501 caccacgagt agtagagtca gctaaagcag aaaatgcaga tgtcatgctt gtggatcata
    13561 atgaattcca acaaagtatt tcagatatca gcgaagtaaa tattttagct gtagtagacc
    13621 accatcgtat tgcaaacttt gaaacagcta atccattgta ctatcgtgca gaaccagttg
    13681 gatgtacaag tacgattatt ttaaaactat ataaagaaaa ccaagtaaca attcctaaag
    13741 aaatcgcagg aatcatgctc tcagctatca tttcagatac attgttgttc aaatcaccaa
    13801 catgtacgcc tgaagatgaa gcagctgcaa aagaacttgc agcaatcgct gaagtcgatc
    13861 taaacactta tggacttgaa atgttgaaag caggaacaaa tctaagtgat aaatcagctg
    13921 cggttcttct tgatttggat gcaaaaagtt tcccaatggg cagttccaac gttcgtatcg
    13981 ctcaagtcaa tacagtggac cttaatgaag tgatggatcg acaagcagag ctagaagcag
    14041 caatggaaga tgaaatgaaa gcaaatggct acgatctgtt cgtattagtt gtcacaaaca
    14101 tcttagacag tgattcagaa ttacttgttg ctggtaaagg aaaagaaaaa gtagaagaag
    14161 ctttccaaac aactttgacc aacaaccgtg ctttgcttaa aggtgtcgtt tcacgtaaaa
    14221 aacaagtcgt acctcaattg acggctgtat ttagctaatc aaatggtagt atgaagcatt
    14281 caaaatgaat ggataagcga ttgatataaa aagaaacata gaacatacta aaggccgaag
    14341 aaatggagga gaataacagt caattatgac tcttgttctt cctccttttt acgtaaaagc
    14401 cttttaagta aaataaaacg gatagaaaag agaacatata tgaactatta ttcaaaaaat
    14461 aaggaaaact tttatcaaaa actgaccgat gatccgcttt tttcttcatt gaccgattat
    14521 ctatatgaac atcgagaaca agaaacgatt ctaagagaat tgaaaaaaga attttcgcaa
    14581 aataaatttt cccattttct tgatttattg atcgatgcag gattaatcaa gagggaagaa
    14641 cgaagatatc atttgaactt tccaatattt gatcctaaag attacttgca acaagccact
    14701 tcggctgctg aaaccatagc tgaacaattg aagagactct ctgttgatga acaaaaatta
    14761 gcgatgggag aagttatttg ggcgtattgt ttcgaagatg aacggaaaga agcgtatttt
    14821 tatggagtaa gaaacagccg agagacagaa ttgctgagga caactgcagg aaatgagaag
    14881 taccgtttta tcacactttc ctctatagaa cacttcccgc ttactttggc taattacttt
    14941 tttgtacaga agaatcagct tcctgtaacg aaggcattca aagaattagc cgaacttatt
    15001 ggagacgtaa atgaatcgta cttttttgat caaatagaag ttatcgttga caggatacgt
    15061 aagaataaat ataaaaatcg ccggcctagt attttccatc agtctctact tgtgacgaat
    15121 actataaagg aagaagaatc attcacatta gaattaccta ttgtcgaaaa aaacaatttt
    15181 gagattgaat taccaacatt ggatccttca ttgacaatgg aggaaatggc ctttcttaaa
    15241 cgacaaatct tttctgagct gagcaagaaa tttataccgc atgcattttc ttatatcaaa
    15301 gaatatggaa cagttttagt gagtaaaacc taagagagca gtaagcaaaa attatactga
    15361 ctcttgcagg aggagccttt ctcttccttt atggttaaaa gaattatttg agtattttgc
    15421 ctatctacca cctattagat tcactacatg gtctggaaac aaaggaatgc aaaaaaatgg
    15481 tgagaaatgt ggaatgacaa aggaagtagt gataagaaaa gttagatttt taaataatca
    15541 atattatgat tcagtcaagt atggcatttt atgggaagaa ctagctgatt agattaggaa
    15601 gtccatttga taatccagaa tcactaagaa acgaatggcg agtcattaat aatttatcca
    15661 atcttgggtt attcttttag attgaagtgt ttaaaactaa aaaagcattt atgaagtcgt
    15721 aatagtcagg cttcaaaatg ctttttattt tgtttgtttt ttctttgcca cataaataca
    15781 cctagactga cgatgaagag catatccaac agatttcctg cgacttggat cgtcgaaggg
    15841 ttggtcagtg gcattaaagc aaggacaaaa aaggaaaaca agctgtaaat aaaacaggtg
    15901 acaggaacta atcgaaagaa gagcggacat ttcaattggc taaaaatgag tgaaaaaata
    15961 aaataagtaa aaatggataa aatcacgact aaaccattca tgataaagaa gaaaagcgta
    16021 ttgtgattaa acgaataaaa aagaattgct aaaaaagaaa acgttttaaa tgcaatcaat
    16081 acgcttgaag cttcgtttaa cgtaccagga tgtcgatgaa aaaaataggc accgaaataa
    16141 acagaaaaca gtaagccgca aagagaagac aaaatcaaaa aaaacggatt ttcttcggct
    16201 tctgtgatat cagaatgatc caaaagcaac cagcctaatg cactagaagg cgcatgtgag
    16261 gtcttaaggt atgaataaca tagaaaaaat aagatcgtca aagttgcaaa aaaagagcct
    16321 aaatgtaaaa aattagtggt tctacgttga atttccataa tatctccaaa ttaaattgat
    16381 aagtatataa agtataataa caaattgatg agtatatgtc gatgtttttt aatcaaaata
    16441 ataaataaaa actgtctaac atttaaaatt taatttagat attagacagt ttttaagttt
    16501 gtgagttatt ttccttgtcg ttttctcact tttttagtcc agaaaccacc tgaaatatat
    16561 tttggctcat aagaaatgat aaaagcacgc tcttcgattt ctttgatcag gcgatacagt
    16621 tcacgttcgc tttttctagg agaaagtatt tcgagaacta gccgctcacc ttccagcccc
    16681 attgcagaag tctgtgtcac accataaccg tgttctctca gtatacccgg caaactttta
    16741 tcttctgcgc tatttgctgg caaaatcgtt gtgaccatga tgtatcctaa tgccaatttg
    16801 tcttcaatca aaattccgac actgatacct actgcgtaac ctaacgcata gacaaataaa
    16861 ttcagtggat tatctaaacg atctaaaaca agggataagc cgacgatata aatcgtgatc
    16921 tcgaccatac tgacaagtgg agcaatcagt cgatagcctt tcattgtcaa catgaaacgt
    16981 aacgtgttca gagtgatata agcaaaatta atgaggaaga tcatccccaa catttttgga
    17041 tcaatcattt gtaaaactcc tttcatttat ataacaacaa tatcaaaaaa aatcgtaagt
    17101 gaagaaaaat tgaaacttta tacatacaaa aaaatgataa aatgattata atagaattat
    17161 aaagataggt taggtgatta aaatgaaaac ggtcaatcgt gtgttttgtg ccagccacgt
    17221 gctaagtctc cttctcagta tatacttagg tgtctattcg attcaacagc caattgaggt
    17281 agagaacatt gtttttcaga tgtcgctgac agatggttta attttaagcg tttttttctt
    17341 tttgatactg tttattggta atattttcgg tagcgttgcc agttttttga atttttctat
    17401 ttatccactc ctttcgctag cattaggagt ggtgggactt tgttctctgt gcttatttcc
    17461 cgagccattc agtcctgcat ttatattatt tggtacactg aacctgttcc agacaactgt
    17521 tggagcatgg ttgctatggc gatcaggaaa tttaatgaaa ataggtgagt aacaattgaa
    17581 tccaattata ttgaaacgtt ctgtccatca tattccagtg ttagaagttg ttctgaaaga
    17641 attaagagag gaacgtattc ccgttgtcat ctattatcac ggatggcaga caagtaagga
    17701 gttagttttg acgcaaggaa gaaaaattgc gcagcagggc attcgtgtga tcctgccaga
    17761 tgcgatgaat catggggagc gcaaacagcc ggtttcttct attccatcct atacattttg
    17821 ggatagtatc tacggaaatc tgtttgaatt tgacacactt atcaagcatt tagaaaaaag
    17881 aggtcttcta catgatcagc ttgcagttgg cggggtttcg atggggggaa tgacaacatg
    17941 cgccttgttg gctaaacatc ctcatatagt agcgggcgtg tgtttgatgg gttcccctgc
    18001 acctttggaa tatggaaaag aaatcgtaag gcgcgcatcg gaatataatt atgtcgtacc
    18061 aaaagactat tttgatttga tttcatgggt taagagctat gatttgtcat tgcagccgga
    18121 aaagttagct ggacgtcctc tgttcttttg gcatggacaa gctgacgaaa aaatccctta
    18181 tatacaaacg gaacgatttg tagaaaaaaa tcggcataag tcttacggaa aagaaattgt
    18241 ttttcgttcc tcaaaaacac agcgacatat ggtacaaata ccactaatgg aagaagcttc
    18301 cactttttta gcaaaaaatt tgataaaaaa gtataaataa ttgaaacgga aaatcactca
    18361 tgacgataga ggattttccg ttttttattt tgggctataa taaactctgt taggaaaatt
    18421 tggtataatt gatagtaaga atgacaacat gttagaaagg aagagagcat gcccacttta
    18481 gcagatgttg caaaacgtgc caatgtttca aaaatgacgg tctctcgagt catcaatcat
    18541 ccggaacagg tgactgacga attgaaagag ttagttttct cagcaatggc tgaactggat
    18601 taccgtccaa atattgctgc aaaagcactt gtcacgaatc ggtcgcagat catcaaatta
    18661 tttatactgg aagaaatcga cacaactgag ccctattaca tgaatttgct gatgggaatc
    18721 gctaaacgga tcggtaaagc gcattattct ctccaattag ttacaaatga tgccttcgac
    18781 gtcggttctt gtgatggata catcatcaca ggtgtcagaa aaaaagactt cgattggatc
    18841 gaccgtttgg aaaaaccagt tgtcttgttt ggggaaaatt ctgaaggata tgattatgta
    18901 gacagcgata atgaatatgg tacagcaaaa gcaacgcaat atgccttaga aagagattat
    18961 caaacgatta tttatatcgg tatcgaagtg gacgagcctt ttgaaatttc aagggaaact
    19021 ggttatttat ctgtgatgaa ggaagttgat cttcctagta aaatcttgcg gtttccaaat
    19081 cattcgacag aagtagaaag atatatgaca gaaaactggg cagattttac agataaaact
    19141 tgttttgtat gtagttcaga tcggctagca ctaggagcag taagagggat tataaatgct
    19201 ggaggacact tgcccgaaga atttggtgtg attggatttg atggggtatt tttagaccaa
    19261 atcagttcac ctaaattgac aacgatcaag caagatatta tacgtatggg tgaagtatgt
    19321 ggagaaatgt tgttaaaaaa aatagaagaa aaaggggcga gtcaaggtta tcgtcgtttt
    19381 tttcctgaac tagttatcag agaga
//
LOCUS       DACECZ010000043        17995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.043, whole genome shotgun sequence.
ACCESSION   DACECZ010000043 DACECZ010000000
VERSION     DACECZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.043"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            214..498
                     /locus_tag="IWO35_001416"
     CDS             214..498
                     /locus_tag="IWO35_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ0316478.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="IWO35_001417"
     CDS             complement(779..1198)
                     /locus_tag="IWO35_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ0316479.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="IWO35_001418"
     CDS             complement(1200..1784)
                     /locus_tag="IWO35_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ0316480.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="IWO35_001419"
     CDS             complement(1831..3306)
                     /locus_tag="IWO35_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ0316481.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="IWO35_001420"
     CDS             3487..3798
                     /locus_tag="IWO35_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316482.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="IWO35_001421"
     CDS             complement(3913..6249)
                     /locus_tag="IWO35_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0316483.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="IWO35_001422"
     CDS             6391..7059
                     /locus_tag="IWO35_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0316484.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="IWO35_001423"
     CDS             7062..8105
                     /locus_tag="IWO35_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0316485.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="IWO35_001424"
     CDS             complement(8342..9223)
                     /locus_tag="IWO35_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0316486.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="IWO35_001425"
     CDS             9382..10110
                     /locus_tag="IWO35_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ0316487.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="IWO35_001426"
     CDS             10266..10583
                     /locus_tag="IWO35_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0316488.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="IWO35_001427"
     CDS             10576..11184
                     /locus_tag="IWO35_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ0316489.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11200..12030
                     /locus_tag="IWO35_001428"
     CDS             11200..12030
                     /locus_tag="IWO35_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ0316490.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            12168..12656
                     /locus_tag="IWO35_001429"
     CDS             12168..12656
                     /locus_tag="IWO35_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ0316491.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="IWO35_001430"
     CDS             12707..13579
                     /locus_tag="IWO35_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ0316492.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="IWO35_001431"
     CDS             13755..14117
                     /locus_tag="IWO35_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0316493.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="IWO35_001432"
     CDS             14126..14878
                     /locus_tag="IWO35_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0316494.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="IWO35_001433"
     CDS             14892..16694
                     /locus_tag="IWO35_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0316495.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..>17995
                     /locus_tag="IWO35_001434"
     CDS             16971..>17995
                     /locus_tag="IWO35_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0316496.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYK"
BASE COUNT         5637 a         3127 c         3721 g         5510 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatac tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaat
//
LOCUS       DACECZ010000044        17984 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.044, whole genome shotgun sequence.
ACCESSION   DACECZ010000044 DACECZ010000000
VERSION     DACECZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.044"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..827)
                     /locus_tag="IWO35_001435"
     CDS             complement(<1..827)
                     /locus_tag="IWO35_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ0316497.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDV"
     gene            complement(1131..3296)
                     /locus_tag="IWO35_001436"
     CDS             complement(1131..3296)
                     /locus_tag="IWO35_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0316498.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            3556..5850
                     /locus_tag="IWO35_001437"
     CDS             3556..5850
                     /locus_tag="IWO35_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0316499.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5843..6508
                     /gene="pgmB"
                     /locus_tag="IWO35_001438"
     CDS             5843..6508
                     /gene="pgmB"
                     /locus_tag="IWO35_001438"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0316500.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            6501..7508
                     /locus_tag="IWO35_001439"
     CDS             6501..7508
                     /locus_tag="IWO35_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0316501.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            7530..8549
                     /locus_tag="IWO35_001440"
     CDS             7530..8549
                     /locus_tag="IWO35_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0316502.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(8830..10197)
                     /gene="nhaC"
                     /locus_tag="IWO35_001441"
     CDS             complement(8830..10197)
                     /gene="nhaC"
                     /locus_tag="IWO35_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0316503.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(10306..11727)
                     /locus_tag="IWO35_001442"
     CDS             complement(10306..11727)
                     /locus_tag="IWO35_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0316504.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(11970..13847)
                     /gene="tdc"
                     /locus_tag="IWO35_001443"
     CDS             complement(11970..13847)
                     /gene="tdc"
                     /locus_tag="IWO35_001443"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0316505.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(14138..15394)
                     /locus_tag="IWO35_001444"
     CDS             complement(14138..15394)
                     /locus_tag="IWO35_001444"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ0316506.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            15974..17275
                     /locus_tag="IWO35_001445"
     CDS             15974..17275
                     /locus_tag="IWO35_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0316507.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(17344..17943)
                     /locus_tag="IWO35_001446"
     CDS             complement(17344..17943)
                     /locus_tag="IWO35_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0316508.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         5472 a         3821 c         3257 g         5434 t
ORIGIN      
        1 ttcacatcta attcgatttc caccccgtag tgatcactga tgattggact atcgatccca
       61 tcaaaaatcc gttgatattt tttaacctgc attccttcag ggacgaaaat aaaatctata
      121 cgcaattttt ctgtattttg ttcccaacca tctattttct tttccacagt agctgctttt
      181 cctttttcat tcgcgacaac atacgaatct tgaagaagaa ggcaactttc tcttacagta
      241 taataacctt gagagtctac tggattattg aaatcaccta ggaaaaaaag tggtacgcga
      301 gtttccaaaa agtagttttc caagtttttc cactcaaaat aaaagccact ttttttatct
      361 gtccaccaag aaaagtggca gctcgagaca aaaatgtcct gatcatctat ctttgttttc
      421 ccaatcaaga tcttgcgtgt ctgataatct tctttctttt gaaattggga gacggtaagg
      481 acctgacttt ccaatgtttt tttcgtaagc agtgcatttc cttcttcata tttatcataa
      541 ccgatgtggc tcatttccca actccaaaaa tactcttttc cgtgttcctt taaaagctga
      601 actaaacggt aggcaaaatt atctttacgg ataggtactt gattctttac cggacagaac
      661 ttcattagct tcgcaggttc tacttcttca gattccagta attggttgat ttcttgtaaa
      721 gcaatcactt cataatcttc taacaggatc ttatctgcta attcttgcaa ttttttctct
      781 ggttctggct caagccagct atgcgtgttg agtgtcatga atttcattgg ttttctcctt
      841 gtcttcattt cgaaacaaaa gggttgtgac aaaatttttg tcacaacctc tccagcaaat
      901 agtgttctcc tgctaactcc tcagtaataa gctcaatgga ccaggagcta ttttttgttt
      961 ggctgttttt atcacttggt gcagggcagc tttttcacga cctctccact aaacagtatt
     1021 caaactgctg cttctcggta ataagcccaa ccaaacgaaa aataggagga gctatttttg
     1081 tttggctgtt cttatgactt gatgcaggac agctttttca caacctctcc ttacatttta
     1141 atggagccga tcactttacc agctgtcact gtacctaatt gcttgatcac tacttcttct
     1201 actttatcgt ttgtaaatac gatcatgatc gttggatctt tttcttcgtt ttggatcaca
     1261 tcaagatcca tctcagctaa tttcgttcct gttcctactt cttggccttc tttgacagca
     1321 atctcaaaag ctggctgatt catttggact gtatcgattc ccatatggat caaaacttcg
     1381 ataccatctt tcgtttccag cccaattgca tgtttgctag ggaaaatact tgtcactttt
     1441 ccagcgatcg gcgcaacaac ttctcttgat gttggaataa cagcaaagcc gtcacccatc
     1501 attttttgtg agaataccgg atcttttact tcagataaag gaatcacttc tccagaagct
     1561 gcggaaacta gttcttgttc tatacctaag tattgcttac tacctgcttg tccatttcct
     1621 gtagaaacca tttctggctc tggaatctct gctcctgatt gcagcagatc ttcgatatct
     1681 gatttcaaga catctgcttt tggtccataa actgcttgga ctccattgtc tttgacgatc
     1741 aatcccatcg cccctgctcg tttccaggct tcttcagtcc ctactttttc tcgatcattt
     1801 acacttacgc gtaaccgagt catacaagca tcgacatctt tgatattttc ttttcctccc
     1861 agcaaatgaa tgatctggac cacttgctga tctgctgttc ctgttccgct gttagataca
     1921 acggtttctt cactgtcatt gtcgtaattt ccgttacgtc ccggtgtagc aaagttgaat
     1981 tttttgatca agaaatttgc taagaaatac gtaacaacac caaaagcgat cacacacaac
     2041 acgaagttga taagatctcc acctagaccc gctttgatag ctaacggtgt ccgagtcagc
     2101 aattcgatat tcccaaatga atggacccgc aatggcaaga tatctgccat cgcaaatgca
     2161 gccccttgga tcactgaata aatgacatat aatggcacag cagcaaacat aaacatgaac
     2221 tccaatggtt ctgttacacc tgttagaaag acagccaacg ctgctgaaaa atacattgat
     2281 ttatatcttg ctttcttatc aggatcgaca ttacgataca tcgcaaaagc cattcccatc
     2341 aagatacctg aagaaccaat catttgtcct actttgaaac gagccggcgt ccagttatgc
     2401 aacacaaact catattgcga catatcccct gcacctttaa gattcactaa atcagtggcc
     2461 caagccaacc ataacggatc ttgcccaaat acttgtgtcc ccgcctgtgc gcctgataaa
     2521 atctcatacg atccgccaag ctgtgtatag ttgataggaa tcgtcagcat atgatgcaat
     2581 ccaaatggca atagtaaacg ttctaatgtt ccaaataaaa acggtgctag aatcggtgcg
     2641 ctttcttgtg attctgcaat ccataaacca aaattattga ttccagcctg tacgtttggc
     2701 cacaccacag caagaatcaa tgccacgatt gttgaccata agataacaac aaacggaaca
     2761 aagcgctttc cattgaaaaa tgacaaagca tccggcaatt ttctataatt atagtactta
     2821 ttataagcca ttgccccgac aaatccagcg atgatcccta cgaatactcc catattcaaa
     2881 gctggtgctt ccaatacact tgtaaaaaat cctttaacca tgatcttcgt accgaacaga
     2941 gtgtgggtaa atgcatcttc attcgcaagc atgtcacttg tgacaccaaa gatagcccca
     3001 gtgatccgat tgattaaaat aaatgagatc ccagctgcaa atgctccgcc tgctctttct
     3061 tttgcccaac ttcctcctat tgctaaagca aataaaatat gaagattacc aataattgcc
     3121 caaccaatat tttcaattac tccgcccgtt gtgacaagcg gcgtccaatc aggattgatc
     3181 aagggaattg atttccctat actgatcatc aacccagctg ccggcataac tgctaccaca
     3241 accattaaag cctttccaaa tttttgccaa aactcaaaac taaatatctt tttcattccg
     3301 tttagctcct ttacgcaatc ggtttcgtta aacgtattat agcgtgcgtt ttcttttttg
     3361 taaatgcttt attttttatt tttctgtcta taccattcta taaaacctat aatacgtgtg
     3421 ttcccttcta taattttctc ctaaaaattt atttttttaa gcttttttct atgaaatatt
     3481 ttcttttgtc tattgacttg tgcaatcggt tgcgtttatt atgaacgtat aaaacagctc
     3541 attaggagga gaaaaatgaa acagatcaaa cgactatttg ctattgatcc atggaaaatc
     3601 agcacgacac aattagataa aaaaaatctt cgactgcaag aatctttaac aagtattgga
     3661 aacggttata tggggatgcg cgggaacttt gaagaaactt actcagggga ccaccatcaa
     3721 ggtacttatt tggcgggtgt ctggtatccc gataagacac gtgtcggctg gtggaaaaat
     3781 ggttatccag aatactttgg aaaagtgatc aacgccttga actttatcgc gatagatatt
     3841 tcgatcaacg atcagctgat cgacttggct tctttggaac cagaagactt ttactgggaa
     3901 ttagatatga aaaatggtgt attatctcgt acattcacaa tcactacttc tactaataaa
     3961 gtacgcattt ccttcgaacg ttttctgagc attgtcaaaa aagaatcagc ttatatctat
     4021 ttaactatag aaatgcttga aggtagcgga acagtcaaac tcgtatcaaa attggatgga
     4081 aatgttcaaa acgaagacag caactatgat gaacattttt ggaatgagat tgatcgtgga
     4141 actatagaag aaatcggcta tttgacgact aaaactattc caaacaactt tgggattgaa
     4201 caatttactg ttactgcagc aatgcgccat ttcgttaatg gaaaatcagt catccctgac
     4261 tatcacgaag atactttagc attacatggt tgcttgtctg cagaattatc tcaaggtgag
     4321 acatggtcag tggaaaaaga aattatcgtt cttactagta gagatattcc agaagcgcag
     4381 caagcagaaa aagctgtctc agaattaacg aaccagacaa acaattatat ccaagcaaaa
     4441 gaagagcaaa cagcggcttg ggcagaacga tggaagcttg ccgatgtggt catcgaagga
     4501 gacgaagaag cacaacaagg tattcgattc aatttgttcc aactattctc tacttactac
     4561 ggcgaagatg aacgattgaa tatcggcccc aaaggattca caggagaaaa atacggcgga
     4621 gccacttact gggatacaga agcgtatgct gtccctcttt atcttgcttt agccaaacct
     4681 gaagtaacta agaatctgct gaaatatcgg cataatcaac tgccgcaagc aatccacaac
     4741 gctcagcaac aaggattaaa aggcgcgctt tatcctatgg tcaccttcac tggtgtcgaa
     4801 tgccataacg agtgggaaat cacattcgag gagattcacc gaaatggcgc gattgcctat
     4861 gcaatctaca attatacgaa ttacaccggc gatacggaat atttgaaaaa tgaaggactg
     4921 gaagttttag cagaaatcgc acgcttctgg gcagaccgtg tccatttttc aaaacgagca
     4981 aataaataca tgatccacgg tgtgaccggc ccaaacgaat atgagaacaa tatcaataac
     5041 aattggtata caaatacgat cgcagcttgg gtcttgcgtt acacgcgaga aaattattta
     5101 acttatcaaa aagaagctgc tatcactatc agcgaagaag aactagccaa atggaaagat
     5161 atcgcagaga acatgtactt cccagttgac gaagaattag gaatctttgt ccagcatgac
     5221 acattcttag ataaagatct catgcctgta tccgatattc ccacaagcga tcttcctctg
     5281 aatcagcatt ggtcttggga caaaatcctt agatcttgct tcatcaagca ggcagatgta
     5341 ctacagggga tttatttctt caatgatact tttacaaaag aagaaaagca aaaaaacttt
     5401 gatttctatg aaccaatgac tgtccatgag tcttcccttt cgccaagtat ccattcgatt
     5461 ttagcagcag aattaggaat ggaaagcaaa gctgtagaaa tgtaccagcg aacagctcgc
     5521 ttggatttgg ataattacaa taacgacaca gaagacggct tgcacatcac ttccatgact
     5581 ggtagctggt tgtcgattgt ccaaggattt gctcaaatga aaactgccca tgatcagttg
     5641 agattcgcac catttctgcc aaaaacttgg aaagcttatt ctttccatat caattatcgc
     5701 ggacgtttat tgttcatctc agtcacagat gaacaagtta ctttcgagtt attagaaggt
     5761 tctcctctag aaatttacgt ctatgaacag tcttattctt tagcggatcg tctagaaata
     5821 acgctaagaa aggaggaagc aaatgtttaa aggggtattg tttgatttag acggtgtcat
     5881 taccgatact gctgaattcc attatcgtgc ttggcgaaag cttggtcagg aaatcggcat
     5941 tacgatcgac cacacattca acgaacagct aaaaggcgtc agccgagaag actcactctc
     6001 tttgctttta gcttatggcg gaaaagaaca ctctttttca aaggaagaat ttgcagaatt
     6061 agcgaaaaga aaaaacgatt attatttaga gatgatccag acgatcgaac caaaagatgt
     6121 cttccccggg gtcgtgcctt tactggattc tttaaaagaa gaaaaaatca agattgctct
     6181 tgcttctgca agtaaaaacg ggccattcct acttgaaaaa atgggtctta caccttactt
     6241 tgatgctatt gccgacccgg cagaagcaac aaacggaaaa cctgcgccag atatcttcct
     6301 ccttgcagca aaagctgtcg gactaaaagc agaagaatgt atcgggatcg aagacgcgca
     6361 agccggtatc caagccatcc tctcaagtgg cgctcaacca attggcgtgg gacgtgctga
     6421 agaccttgga gaggagatcc cgcttgtccc tgatacgact tttcttacga tcgattactt
     6481 gaaagaaaag tggcatgacc atggataatc cagagatctc cgtcgtacca gttccagaga
     6541 ctgaaataaa gaaaatcaca ttgaagaacg actacttaga agtcgttctt ctcaattacg
     6601 gtgcccgcct gcaccaaatc ttcgcaccag acaaagaagg aaaatcagaa aatgttttat
     6661 tgtcttacga tcgtttcgaa gatgttctaa cagacaaaag cttttttgga gcaactgtcg
     6721 gacctgtagc cggaaggatc cgggatgctt cttggggtga gcatagattg gagaaaaact
     6781 gcggatcaca ccatatccac ggtggcacga aaggctggtc tttccaattt tgggaagtag
     6841 aagttttcaa aactccatca tcaataggtg tggtctttta cttaaaagat gaattttcta
     6901 cctatccagg accaatcact gctacgatca cttaccgttt aacaaaaaac gaattagaga
     6961 tgacaacctc tgctagtagc cttgttgaaa ccatctgcaa tccgaccaac catgcctact
     7021 tcaacctttc aggaaatgga acaagagaca tctatgaaca tcagttaacg gtctttttag
     7081 atggtgtgct tgaattagat caagaaaaac tgcctactgg aaattggaca aaaaaagaaa
     7141 aactgccaat cgactttaga aaatccccaa cactgcaaga gatccttgca tgttatccag
     7201 atggactaga tgatgtattt cttttgcatc atcctaggct ttccaaaact tccttacaat
     7261 tattcgagaa gcattctggt cgccaaatga ctatagcaac ttctaataaa agtatggtat
     7321 tattttctac gactggattc gaagctgatt tctctgtcaa tggaaaacag atgcattcaa
     7381 actacggatt agcaatcgaa ccgcaagaaa tccctgatat cgttcatttc cccaaatttg
     7441 gatcgatcaa cctccatcct ggtcaagaac ggatcagtca gacgatttat cggttctctg
     7501 cacaataaca agagaaaggt gtttttatca tggctattac cgtaaaagat gtggcgaaaa
     7561 aagccggtgt tgccacctcg accgtctctc gtgtcatcaa cgatcatcct agtatttccg
     7621 agacaacaaa gaagaaagtc cgaaaaatta tggatgaact aggttatgtt cctaacatga
     7681 ctgcccgcaa tctaggaaaa cgcatttcca gtgccatcgg cgtaatctta ccccctcttg
     7741 attcaaaaga gcggatcggt aacccatttt atcttgagat catggaagca atcaacgaag
     7801 aagcgcggaa ttatgatatg actacagcaa tcgctactgc taaaaatttt gaattgctgt
     7861 tagagaacgt ccagcgtatg catttacaaa aacaagtaga tggttttatt ctagtgtatt
     7921 cagatagtca agatactgtc attgattatt tataccaaaa ccacatccct ttcactttga
     7981 tcggacagcc ttatcatcat gagaacgaaa tcgtttatgt agataatgat aaccagcttc
     8041 taggtaaaca agcaacagaa tttttgatcc agaatggaca taagaatatc ctgtttgtga
     8101 caaacacgac tcatgaaagt ttgtatttcg aacgatattt cggttatcaa aaagcaatga
     8161 tgatggctgg tcttcctgcg ttatccagtg tagtactaga gcatccggag gattatctgt
     8221 cctttgagaa aacgttgaaa gaaaacgaag ccacagcatt ggtggtcatt gacgatatct
     8281 tcgcccttcg cacgatgcag ttggcgcaaa tgtatggcta tgtcattcca gagacactct
     8341 ccgtcattag cttcaacaac tcgatcttct caacattaac acacccttac cttacaagta
     8401 tcgatatcga tatctccgaa ttaggacgtg tcgctatgca aaagctgcgc gaattgatcc
     8461 atgaagaatt gagcagtggc gtacgtttgg tcatccctca tcgcttgatc aaaagagaga
     8521 cagtcgttcc tttagaaaac ccatactaaa tagctgactc actaaatacg ctgaccattt
     8581 atcctatggt cagcttttta tgtaaaagaa gatactactt ctaacacatt gtttatctgt
     8641 aaaaaagagc gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa
     8701 acggcggaag cagaagcaac tccttcggaa ataagctgaa attcacaaaa atttaaaaag
     8761 caattttctt gagtttcttc ttatttcttg gagttaaacg cttctgtccc agcctctttt
     8821 ttccttcctt caatttttta cacgtcgacg attcaataaa aaaccgccta atattgtaaa
     8881 gacaggtgca agaatcgaga aaaaggcgaa tggcaagtaa tgcaacgcgc taactttcat
     8941 cgttcccata atgaatgtgc cgctgactcc ccacggtacg agagaattga cggtcgcacc
     9001 tgaatccgct agtgttcgtg tcaaataggt ttttgataag ccaacgtcat caaaagaacg
     9061 cttgaatgtt tctcctggaa gaatgatgga cagatactgt tctccaacga tcagattgat
     9121 tccgatacag ctcaaggcag taaacccgac taaacgagaa ggtcggtcca tcttttcttt
     9181 taattcttga acgatggtat caacgatcag atatttgatc agtaacccac ctaatgccaa
     9241 tgcaagtaaa atcaaactag cagaacctaa catactcatg atcccgcctc gagaaagcag
     9301 ttcatctagc tttggatcag gtgtatttgc gacataacca ttcatcaaga tgtctgctgc
     9361 ttttccgacc gtcaaacttt tctcatggat aaaagaaaga acgagagcag ctgtcgatcc
     9421 cgctagtaat gtagggatag ccggaatttt tttgattgcc cccccaaata aaataactag
     9481 tggaatcaat gtccaaggag agatccaaaa gctcgctttc atgttttgga tcatttcatt
     9541 gatactagct agatcggctg aatgactgtt atgtcctaat atcgtataaa aaatcaacga
     9601 aaggacgaat gctgggaaat ccgtataacg cataccagcg atatgttcga atagattaac
     9661 tcctccaata cccgctgcca agttggtcgt atctgataaa ggcgagcagt tattccctaa
     9721 aaaggcaccg gaaacgactg cgcctgtagt cattgcatca ttgaatccaa gtaaatgacc
     9781 gatccccaaa aaagcaattc caatcgtcga aatcgttgta aatgaactgc cgacagtcac
     9841 tccgacaatt gcacaaacaa gaaatgctgt cggtaaaaag aaacgcacag aaacaagttt
     9901 aaatccataa accataatcg tagggatggt tccagataaa atccatgaag caactaagac
     9961 accaatcaaa agaaaaatca ggattggaat gatccctgga gtgattcctt tgatgatccc
    10021 gtcatggatc tcatcccatg tgaacttttt aaatctgcca taaaacaaca aacacatgaa
    10081 agctgcaagg atcgggatat gtggcgagag ctggaatccg atgatcagta ctcctaggag
    10141 tgccaacaac acgatcaata cagcgatact ttcttttaaa gaaatatttt tattcatcgt
    10201 cgatctccta tttcaaatga aaaggccctg ccatgaaaaa actattcact tggcaggacc
    10261 tcctactaaa tcagcgacaa aacgtcggct gaaagactta tttttttatt tttcttctaa
    10321 acttttttct acctcaatcc cccaagaatc tttcttgaag tgattgatga tcaatggaat
    10381 aacgaacatg accactgtga tagctgctac taataatgca tcactcaatg tcgctgtacc
    10441 taatgtagca aatacgacaa cgatgataga gaagatcagc atgcagctga ctacccaagc
    10501 taatccgttt ccgtttttac caattcggta aggacgttcg aattctggat gtttcttacg
    10561 cattttaagc aatgaaacag caatcaaaat gtacacgata caataaataa cagttgtagc
    10621 atttgtaagt gtcaagaata cagcacttac gtcatccatt acaccataaa gcaaagcaaa
    10681 taatgaaata acgattgatt gtgttagaac gacatttcta gatacgccgt atttgttttc
    10741 tttatggaat ccgaattttg gtggaaggaa tccttcacga gctacttgga tgattgtttt
    10801 tgatggtcca gtaacccaag ctgataattg taaaagcaca ccaataaata ccatgaagct
    10861 gaagatattt ccgatcactt ctggtaagcc taggatttga cagtcaatca agataggctg
    10921 cgtgatattt gctaattcca tttttccatc tggtacgaca tttgctacaa gcatcgcatt
    10981 gatgacattc aataaaacta atccgatcaa agcgataaat acgccttttg tataattttt
    11041 tgttgcgtct ttcaaacgag gcatgtaaac agaactcatt tcgatcccaa caaaaataaa
    11101 tgtgatacca gctaagtatt tcaatgaatc aatatgttcc aagtcaggga aagcttttga
    11161 ccatgaaaag tcacctagat agccgtttga gactagtccg actttgaagg ctgccaaaac
    11221 acctaaaacg aacatgatga ctactgggat atatacccct agccatacac cgatgttccc
    11281 gccgactttt gccatatcga atttcaagtt caagatcgtg atgatccagt agataactaa
    11341 aatacaacct aaaacgaacc agtgattatt gccaagttct acattaccaa atgtgtttcc
    11401 taacaatggt cctaaagtag aagcgaccat taccatacct gggaacattt gcacccataa
    11461 tagccatgca acgacaaatc cccatttgct gcccagtgct gtttttaccc ataactgagg
    11521 accgccttct tgaggaagca tcgtggaaag ctcaccggaa accattgata tcggaccggc
    11581 aaaaaacaaa actgcaaaaa ttgaataaaa aatcaatgtc caaccgactg cggctaacgt
    11641 aggaatactc cgaaccgttg cacacagtgc cattgtcaga ccgataaagg tcccaagacc
    11701 cattttactt acttcagatt tactcaccgt tgacactctc cttgattttt tggataaaat
    11761 aacggtgtct ttgaagtagt ttcagcaata aacgaaacaa ttcagcgaat atttgagggg
    11821 attctttgag tttattgctt gaagggtttc ttcaaagaca acctcaaaac aaagggatcg
    11881 aagagcttag gtctcccctt gctcttcaga tccaaacatg cgtcagaaac agttagtatt
    11941 caagctgttt tctaacatga ttaccttgct tattttgctt cgcttgccaa taattggtct
    12001 gcgtagattt tttccaattt ttcttgaaga gctgctttga ttttttcagc atattcttca
    12061 aagtgttctt ctttgttcat gtatggtgtc attacgctgg cacgcaatac agttactttt
    12121 ccggcacggt tccattcttc atctgagaat cctagttggt tcacaaattg taatgggctg
    12181 ttgccatagt ctgggatagc aaagtcagta tgtgaagtta agaactcatt accgtaaatg
    12241 cttcctttca cgtatgaaga ataatcatat acgtcatggt tcaatttatt cattgcaact
    12301 aagtcatcgt tgcctttttc tttgaataca tagtctacca tattgaaatc tggataagtt
    12361 aatggatgaa cttcgatttc tttgtctcct actttaaatg acaagtcatt caagaagttg
    12421 tagaaacgat gtgcaccttc gattgatgca cccattaatt taccatatcc tgtgacattc
    12481 aaaggtaaga catggtgtgc tgcccaaacg ctggctgctg ttgcccctgc ttttgaacct
    12541 tccaagatat aagccccaag taatgctggg atatctgcgc ctttttcaaa tacatatgtc
    12601 gcaaagtaag agataacgtc tctcatgcgg atatctttga tgacgatacc accggcagaa
    12661 tatggaacat agcccatttt atgtgggtca atcgttacag attctgcttc ttcgattgct
    12721 ttatatgcac tgtgtacttc ttctaagata taatctttgt tttctgtaaa gacattgtat
    12781 ttgtaatgaa catcttttaa atcttcaaat ggaatgaagt tgttgtcttc gtctaagaaa
    12841 atcgcacggc cgtatccgcc ataagcagca tctacgtgta agtagaagta aataccgtct
    12901 ttttctaata cgcgacgcaa tgcaacgatt ttatcgatac cgtcgattgc tccttcttct
    12961 gttgatccta caacaccgac tacacctagg attggtgttt tttcagctgc tagaccacga
    13021 acgatttttt caagttcgtt gatgtccatg cgatagttgt ggtcaactgg cactggaatc
    13081 acttggtcta aaccgatacc gatgatgtcg gcagctttta gccatgaata gtgtttagtt
    13141 tgtggaacca accattttcc taatttttct aaatgttttc cgctacgtgc tgagtgagct
    13201 ttgatttcgt cgattttttc tgggacgctg tctaacagat tcatgatttc ttctgttgac
    13261 aagttcatta attcccaatc gctttttcca gcaactagtt caggtgtcac ttctttcatt
    13321 gcaagcggca atgatttgat gttgcgggca taccaaagtc cttctaagtt tgctaaagaa
    13381 ccatcagcaa cgatgtggcc ccagccatct ttatagctca ttaattttgc aaattccatt
    13441 cctacttctt cttccatttg gcttgttgca ggagaagatt cataggcaac gttattacca
    13501 ttccatagca tagcaaaatt gtacgctagt aaagatggca tcaatgtttc agagttcata
    13561 tgaccccagt aacgtccggc attatgccaa ggaacggaat gggtacgcat ccgcgctgaa
    13621 atttctgata gaacatcctt tgttttattc acagtgtgtt caaaactagc actgcttttt
    13681 tcttctggcg taataatagg catgtcttga ggcatgtagt tttgacgcca gcctaaatgt
    13741 tcatccacca attcattcaa caaagcttta tagatttggc cgttctcagc tttgtcccca
    13801 atgaataggg catttaggtt tagatctttc gacaatgatt cactcatagt gaaaccctcc
    13861 taaaaaaata tttaaatttt gtcgagccct acattacacc tgttcttcta ttctgtaaac
    13921 taagttttgg cctgattttt aatcatattt taatatgtat caattatccg aattgttcac
    13981 acttttgaaa taattaaaaa aaacgaggga atttacaaat gtaaactccc tcgcaaagat
    14041 tgtattaacg acaaccagcg ttctatttta ggatgggttt caaaattgga atgttgtaat
    14101 agtatttccc ttttttaagg taaaaactta ctaaattcta atcaattact ttggccaaaa
    14161 agtaattttt cttacctttt cgaataacca caaacttacc atcgaattca tcggatggat
    14221 ttatgacaaa atttaaatcg gtaacgcggt caccgttaat acttatagct ccgtttgaaa
    14281 catcttcacg tgcttgacgt ttagatggtt caatttttgt ggaaacaagc agttccacga
    14341 tattttcagg tgtgcttgaa atttcaacgt tcggcatttt accgaatcct tgcgcgattt
    14401 cttccgcatt tagttcttta atatttccag agaacaatgc ttgcgttatt ttttgcgctt
    14461 ctttcaagtc ttcttcacta tgcacaaatc ttgtaacttc ttctgccaaa cggcgttgtg
    14521 cttcacgttt ctcaggttct gtctctactt ttttcgctaa gtcttcgatt tcttcttgag
    14581 acaagaaagt aaagaatttc aagtatttca ctacatcgcg atcatcttga ttcaaccaga
    14641 attggtagaa ctcaaatggt gaagttttct tcggatcaag ccaaacggca ccgcctgcag
    14701 tttttccgaa tttcgttccg tcagccttca gcatcaatgg gatcgttaaa ccgaacgctt
    14761 ctgcttctgg tccttctaat tttcggatta ggtctaatcc agctgtgata tttccccatt
    14821 ggtctgctcc accgatttgc aattgcacat cgtatgtttt atgcagatgc aaaaagtcga
    14881 tagattgcag gatttggtaa gtaaactctg taaatgagat tcctacttcc aaacggctgg
    14941 caacgatatc ttttgctaac atcgtgttga tgttgaagtt tttaccgtag tctctcaaaa
    15001 attcaagtaa agagattttt gacaaccagt cgtagttgtt cacaaatgtg atatttgcat
    15061 ctttaccaaa caattttctc atttgattgg aaagtgcgtc cacgttatgt tgcacttgtt
    15121 ccatcgtttg caacacacgt tctgttttgc ggccgcttgg gtcgccgatc gttcctgttc
    15181 cgccgccaat cagaatatat ggatgatggc ctgccaattg gaatcttttc atcatcataa
    15241 atggaatcaa atgtccgata tgcatcgaat caccagttgg atcaacgccg caataaagtg
    15301 aaatgctctt ttcttcaacg agctcgcgaa gtctttcttc gtttgtttgc tgattaatgg
    15361 cgtcgcgcca agttaattca tcaataatat tcattttact gacctccgtg tagttttttt
    15421 atgaaagcag gaacctgcta gcatatgtaa ttaattattt cttcaggata ggataggaaa
    15481 taaaaaaagt cccttggcat ataagccaag ggacgaaatc tcgcggtacc acccaatttg
    15541 tatgtctaga tatccaacat acctctcgaa catttaacgc tgtcgcgtgg caatcactgc
    15601 caaagactcc tgaaatgtaa ttcgctttaa agcatgtact ggtttgcagc gaccaccagc
    15661 tttctgaaac attggcttta acaactactt cgtttcgatc ttcgtcttgt tattattaat
    15721 atgtgtctat catagagcgc aactttttct ttgtcaacag aaaaaggata ttttttttaa
    15781 attcctcatt ttttcttttt tgaatccgct ctaattccga acattttatt ttcaattatt
    15841 gttaaaagaa ataacttttt ctgtattttt tctgcaattt atcgagaagt cactttactt
    15901 tgcctcttat tctttcttcg ttacaataag gaatgggaca agtaaaaaaa tgaaaaagca
    15961 agggacgagg tatatggaaa aaaagcaaaa aattatttta ggagccactt ctgctttaac
    16021 tatcgttatt ttaattctaa gtttgttttt tgtcatccga tcatctacaa aaaaagaacc
    16081 aatcattcct gaaaaaaaaa taacagaaaa agcaaaagaa ccgacagatg ataagcaaca
    16141 actagttact caagcaaaaa atctggctca aagctacgat tatgaccaag cgattgcgtt
    16201 attggaaaaa gatagtaaga acggatctac tgaaacacaa cagcttcttc aaacactaaa
    16261 aaaggagaaa gaacaactga tcgactggtc tgatcctact cagatctctc atgtcttttt
    16321 ccacagcttg atcgtggacc cgcaaaaagc cttccactct caacaggcac aaggatacaa
    16381 agattatatg gtgactgttg aagaattcaa ccgttcgatc gaacaacttt atcaaaatga
    16441 ttttgtttta gttaatttga atgatttgat ccaaaaagac gaacaaggaa atctttcctt
    16501 taacgggctt gcgctacctg aaggaaaaaa accattggtt ctttcacaag atgatgtcag
    16561 ctattatgaa tatatggata attccggttt tcctagccga ttggtcgtat caaataacaa
    16621 tgaaatcaaa aatgtctata aagagaaaga taaagaagaa atcggtgact ttgacatggt
    16681 tcctctgatc gatgcctttg tcaagaaaca tcctgatttt tcttatcgag gcgcaaaagg
    16741 taccttggcc ctgacaggtt acaacggagt tttaggttat cgaacatcaa aatctcaata
    16801 tggagacaat gaaaaaacaa aaaaagagat taaagaagct aaaaaagtgg cggaacagtt
    16861 gaagaaagac ggctggagct ttgcttccca tacatggggt cacttgaata tgactcaagc
    16921 ttcactagaa gatgtaaaaa aagacaatga attgtggcag caagaagttg cacctatcct
    16981 aggaaaaaca aatattctga tttatccatt tggtgcagac atcagcgact ggcagcctta
    17041 ttcagcagaa aatcaaaaat ttgcttactt gaagagtcaa ggtttcgata ttttctgtaa
    17101 tgtcgatgct tctacaccag cttggggaca attaggtacg gatttctatc gaaatgctcg
    17161 gatcaatatc gatggcattc gttttcaatc tgatttagac ggaaaaaatc ctattcttga
    17221 tccattcatc aatgtccatg atgtctatga caaagaagca cgcgaatctg cttgatttta
    17281 cgtcatttat gaaccaaaaa agccgaactg acattttatc gtcagtttcg gcttttttat
    17341 gctttatttc ttcttcggtg cgattccctt gatcaggact tctgtccatt ctgctgtata
    17401 tggaagcagt tttgcgtata tctccggctt agaaattcct tctaccattg cttgaaaata
    17461 gaggatcaag aactcatccg aatatttcaa atccactttt ccttctttcc gccctcgatg
    17521 gaaaagatca agcatgatac tgaaactttc ttgcgtatac gcatccatcg cgtcagaaaa
    17581 ttcttttttt tctttttttg tatagtatgt catcaaatcg atataaaatt gtttgttcac
    17641 tcgattgaga taacggattt tgttttcact catggcaatc agtgtttctt caaatggaag
    17701 attttccgca atcaacgctt tcgcttcttg ccctaaatca ttcataaatg caaaaaatac
    17761 ttcataaatc agcccttcct tgctgccaaa atatttaaaa atcgtcgctt tacttacctg
    17821 agcgttctca gctaccttat ccattgtcac tccagcggtt cctatgtctg tgttcatcaa
    17881 gtcaaaagct gcttgtaaaa tctctttctt tttctcttgt gctctctttt caaaaccgtt
    17941 catttgtaga gcctcccgta attttaaact tttataatat gtat
//
LOCUS       DACECZ010000045        17627 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.045, whole genome shotgun sequence.
ACCESSION   DACECZ010000045 DACECZ010000000
VERSION     DACECZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.045"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="IWO35_001447"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="IWO35_001447"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ0316509.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="IWO35_001448"
     CDS             complement(2261..2512)
                     /locus_tag="IWO35_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ0316510.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IWO35_001449"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IWO35_001449"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ0316511.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="IWO35_001450"
     CDS             3461..3679
                     /locus_tag="IWO35_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316512.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="IWO35_001451"
     CDS             complement(3968..4480)
                     /locus_tag="IWO35_001451"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ0316513.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IWO35_001452"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IWO35_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ0316514.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7367)
                     /locus_tag="IWO35_001453"
     CDS             complement(6933..7367)
                     /locus_tag="IWO35_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ0316515.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="IWO35_001454"
     CDS             complement(7377..8165)
                     /locus_tag="IWO35_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0316516.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="IWO35_001455"
     CDS             complement(8162..9103)
                     /locus_tag="IWO35_001455"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ0316517.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="IWO35_001456"
     CDS             complement(9280..9867)
                     /locus_tag="IWO35_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ0316518.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IWO35_001457"
     CDS             complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IWO35_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ0316519.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="IWO35_001458"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="IWO35_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0316520.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="IWO35_001459"
     CDS             complement(12784..13719)
                     /locus_tag="IWO35_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0316521.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13905..14864
                     /locus_tag="IWO35_001460"
     CDS             13905..14864
                     /locus_tag="IWO35_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAQ0316522.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(14950..16044)
                     /locus_tag="IWO35_001461"
     CDS             complement(14950..16044)
                     /locus_tag="IWO35_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0316523.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            16233..16994
                     /locus_tag="IWO35_001462"
     CDS             16233..16994
                     /locus_tag="IWO35_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0316524.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(17109..17549)
                     /gene="fabZ"
                     /locus_tag="IWO35_001463"
     CDS             complement(17109..17549)
                     /gene="fabZ"
                     /locus_tag="IWO35_001463"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0316525.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT         5066 a         3839 c         3088 g         5634 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataacccg cgaattgtaa aattaagtta gaaaaataaa aaggcattta cggtacactc
    13861 aaattgtatt tccgacgaaa gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc
    13921 ttaccataga cgaactgaca atgatagaat catattatct tcaacataat aagccggttg
    13981 aaatcgctaa ccgaatggga cgtgctatac aaactattta taatgtagtc aataagttca
    14041 agcaaggcaa gactgctctt gattattggc accagtataa agaaaataag aaaaaatgtg
    14101 gtagaaaagt cattcaatta cctgctcatg aagtagatta cattaaagag aaagtcactc
    14161 ttggttggac gcctgacgtc attatcgggc gaaaagaaag gtctgtttca tgcggtatga
    14221 gaacacttta tcgtttattt tctaaaggaa tatttgatat tgacacacta ccgatgaaag
    14281 gtaaaagaaa acccaatggc catcaggaaa aacggggaaa acaacagtat cagcgctcaa
    14341 tccatgatcg acctgataat tatcctgatt tcaattctga gtttggtcac cttgaaggtg
    14401 atacgatcgt tggcattcat cataaaagtg ccgtcattac tttagttgaa agattatcta
    14461 aagtcattat cacgattaaa cccaacggcc gtaaggcatt agatattgaa actgccctta
    14521 atcaatggtt ttctcgcttc cctaaaaact tctttaaatc tattacgttt gactgtggaa
    14581 aagaattttc taactggaaa gccattagta accaacatga tattgatata tattttgcgg
    14641 accctggaac accttctcaa cgcccattaa acgagaattc taacgggatt ctgcgtcgta
    14701 atggactgcc gaaatcaatg gattttagaa aagtgaatca gacatttatt tccagtgtca
    14761 gcaatcaacg taatcatatt ccaagaaaat cattgaatta cagaacacca atcgaaatat
    14821 ttttgagcta tgtacaagaa gcattttatt ctagcttaat ttgacaaatc ataacctata
    14881 aaaaaacaaa ataaaaaagc agaaataact ggctatttct agcggattat ttctgcaata
    14941 actaattatc tattttttat tttcaattaa tttaaagctt gaccatggtt tcaaaaagtc
    15001 aaggaggaat aattcgtcat cggagattct tcctacaaca ttgacacgtc cgtcgttttt
    15061 catatccttc aaggcgattt gtgtttctcc tttgtattga ccgtaccctt cattgtcaat
    15121 cagtacatcc ccatgatgca tatctcgcgt attatgaggc gggaaatctt tatctttgta
    15181 gtatacacga gtcatcgttg aacgaaggat atattctgag cgatcccctc gataactatg
    15241 aaggttatca aacaagcaga ttcgttcgtt ttctgtaata ccttcttccg tatctacttt
    15301 caatgttgga taatccgcgt taaatgcttc tgccatttct ttcaattctt cttcacttgc
    15361 gtacgcattt ccaatagaaa tatcatcaat caatcctgta aggattaaat gcttcacttg
    15421 tgtcgcaatt tcaagatctc ggtggtcttc cagcgtgcac aagccatctt gcgtaggcca
    15481 agggccaaac tcagcagatt gcgaattgac gaatgccatc gtattcaaat tatattttct
    15541 gaatttttct gaacaaaaaa caaagtgttc gtatcctaaa ccagagtatc gatgaggata
    15601 aaaattatga gaacctaata aattatccgt gtttggcgaa taactcatga tgttgtcaac
    15661 gtaattcgtg cccgaactca tattgatctc gattttgata ccaaaaggat tgcgcgtcat
    15721 ttttgcttct tctgctcctg tgaatccgat atctaagcgg accccgtagg cacccatctt
    15781 atggaaaaaa gataagtcat cgtaagaaat ctccaactgt tcaaataacg ctggattgat
    15841 atcaaccatt acttccatgc ctaggctatt cgcgtaatca actacttctt taaattctga
    15901 aagaactttt tctttatcat cattgatctg cagcaaactg gtaaaaaccc gtttataccc
    15961 atacttatga gctaaatcca agtacgcttt gtccttttca aatgttgacc gttctggata
    16021 aatcgagatt cctaattttc ccatattacc atttcctcct gcatcttttc gtttttgtat
    16081 ccacttacat tatagcaaac acaaagtaaa tggtctacac cttttctttt tcttttaaaa
    16141 taaatgataa attccaataa tttaagtagg tattttccct ctattttgtt atcattaaag
    16201 ataacaaaat agtaaattgg aaaggaacag ttatgaagaa aaataaagtt attttaagta
    16261 ttagtttagc agcagcattg ctgtttgctg ctggttgttc tgcaaacaac acgacttctt
    16321 ctagttcgtc tacagaaagt tcaaaaagta catctactgc ggaaagcacg actgccagta
    16381 gcgtcgattt gaacgcattg gaattaccac aattgacgaa agatgtcgcc gaaaatgaag
    16441 atctggtcga aatggtgacg aataaaggca caatcaaaat caaattattt cccgaacacg
    16501 caccaaaagc ggttgaaaac tttatgacac atgcaaaaga cggttactac aacggactga
    16561 ctttccatcg cgtcatcaaa gatttcatga tccaaggtgg cgatcctaaa ggcgatggca
    16621 ctggagggga aagcatttgg ggagaaggat ttgaaacaga gatcaacaac cacctttata
    16681 atataagagg tgcgttatct atggctcgct cacaagatcc taacagtaac ggcagccagt
    16741 tctttatcgt tcaaaataca gatgatatgc atgatggtct tttaaaagac gactatcctc
    16801 aagcaatcat cgacgcttat aaaaacggcg gttatccttc actagatggc cattatactg
    16861 tcttcggcca agtgattgaa ggaatggatg tcgtggatgc tattgcaaat atcgaaacag
    16921 atagttcaga caaaccaaaa gaagaagtaa aaatcgataa aatcaatata ctgcaagaag
    16981 caaaacaaaa ataaaaaaat aacgtctgaa tcctgcatag atgacaacgt atgctaggat
    17041 tcaggcgtct tctttttaca taaaaagcaa gtaagcttcc ttttgttttg ctatatcagg
    17101 ttttatagtc aagcattttg gttgacgatg aacgtgaact cgcactcaca gactttttta
    17161 tcatctacat atgctgcaga taacgctgta ccgactgatc ccttcatttt ggtgatttca
    17221 aaatgacatt tcagcacatc tcctggaaca actttttgac ggaatttggc cttattgatc
    17281 ccaccgatat aagctctttt tcctagaaac tcttctgatt ttaggataag tatcgaacct
    17341 gcttgtgcca gtgtttctag aatcaacgca cccggcatca ctggattgtc aggaaagtga
    17401 ccttggaaaa aatcttcatt gattgttaca ttttttgtcg caacgatctt ttttcctggt
    17461 tctaaagtat ctacataatc aatataacaa ataggataac gattaggaat caattccatt
    17521 acttcagtag cagataatac gtaagacata attaaactcc ttctttcagg caattgccat
    17581 tttcattact ttgactatca aagtatcata ttcctaaata tttagca
//
LOCUS       DACECZ010000046        17538 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.046, whole genome shotgun sequence.
ACCESSION   DACECZ010000046 DACECZ010000000
VERSION     DACECZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.046"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            475..861
                     /locus_tag="IWO35_001464"
     CDS             475..861
                     /locus_tag="IWO35_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0316526.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            955..1146
                     /locus_tag="IWO35_001465"
     CDS             955..1146
                     /locus_tag="IWO35_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0316527.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1660..1992
                     /locus_tag="IWO35_001466"
     CDS             1660..1992
                     /locus_tag="IWO35_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0316528.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2288..2443
                     /locus_tag="IWO35_001467"
     CDS             2288..2443
                     /locus_tag="IWO35_001467"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ0316529.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            2719..4059
                     /locus_tag="IWO35_001468"
                     /pseudo
     CDS             2719..4059
                     /locus_tag="IWO35_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4300..5916)
                     /locus_tag="IWO35_001469"
     CDS             complement(4300..5916)
                     /locus_tag="IWO35_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0316530.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            6034..6252
                     /locus_tag="IWO35_001470"
     CDS             6034..6252
                     /locus_tag="IWO35_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0316531.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            6447..6908
                     /locus_tag="IWO35_001471"
     CDS             6447..6908
                     /locus_tag="IWO35_001471"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0316532.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(7101..7340)
                     /locus_tag="IWO35_001472"
     CDS             complement(7101..7340)
                     /locus_tag="IWO35_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316533.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(7364..8887)
                     /locus_tag="IWO35_001473"
     CDS             complement(7364..8887)
                     /locus_tag="IWO35_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0316534.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(8905..9027)
                     /locus_tag="IWO35_001474"
     CDS             complement(8905..9027)
                     /locus_tag="IWO35_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0316535.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(9057..10040)
                     /locus_tag="IWO35_001475"
     CDS             complement(9057..10040)
                     /locus_tag="IWO35_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0316536.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(10064..10213)
                     /gene="rpmG"
                     /locus_tag="IWO35_001476"
     CDS             complement(10064..10213)
                     /gene="rpmG"
                     /locus_tag="IWO35_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0316537.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(10234..10611)
                     /locus_tag="IWO35_001477"
     CDS             complement(10234..10611)
                     /locus_tag="IWO35_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316538.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(10643..10822)
                     /gene="rpmF"
                     /locus_tag="IWO35_001478"
     CDS             complement(10643..10822)
                     /gene="rpmF"
                     /locus_tag="IWO35_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0316539.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(10837..11106)
                     /gene="rpsN"
                     /locus_tag="IWO35_001479"
     CDS             complement(10837..11106)
                     /gene="rpsN"
                     /locus_tag="IWO35_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0316540.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(11629..13401)
                     /locus_tag="IWO35_001480"
     CDS             complement(11629..13401)
                     /locus_tag="IWO35_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0316541.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(13355..15109)
                     /locus_tag="IWO35_001481"
     CDS             complement(13355..15109)
                     /locus_tag="IWO35_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0316542.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(15219..15788)
                     /locus_tag="IWO35_001482"
     CDS             complement(15219..15788)
                     /locus_tag="IWO35_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ0316543.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(15806..17230)
                     /locus_tag="IWO35_001483"
     CDS             complement(15806..17230)
                     /locus_tag="IWO35_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0316544.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(17232..>17538)
                     /locus_tag="IWO35_001484"
     CDS             complement(17232..>17538)
                     /locus_tag="IWO35_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0316545.1"
                     /translation="LSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQYLEFLL
                     VPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFLSILI"
BASE COUNT         5685 a         3314 c         2623 g         5916 t
ORIGIN      
        1 ttattatgat ccgttattat ctaagcacta tacagataat aggaattttt cgtttctaaa
       61 tattccaatt tgggaatcca ataaaaattt tcgttcaaat acgtgtctat tcgaactctt
      121 ttctagttgt taattatgaa gggggaagaa ataaaaaata caatctatac acgaataaga
      181 atattatcca aaaggaggtt ttagaaaaga tttattatac gtaaaaagct caagacattt
      241 tcttagccat aaggacatgt ttgatattta agaagatata tgaattttct tttctaagta
      301 taaagataac tatccattat caactaaaaa aacatttttg aaaataaaca tcagcagtcc
      361 tatcaatgca tgtgagctta tagaagttat tcgcagcgca gcataattgc attagcttct
      421 tttcgtgttt tttgcctctt ttccctttaa atcttaatgg aggaggtgtg caatatgtca
      481 gataacgacg aacgcgtaaa tgcccagttg attcgattct ttgaaaaggt cattttgaac
      541 accgctaata cttattataa aaaacaagaa gaaattcaaa aacatgaaca acttgatcaa
      601 ctcccaccat atttgtttac taaagatact ataaaattta aacaaccgat taatgtttta
      661 aatgtgactc tccttgttga agatataaaa ttagcaaaaa tccttccttt gttgaaagaa
      721 cctgaacaaa tttttcttat cgaaaaattt atctttgata aaactgataa agaaattgga
      781 aaggttctgg ggattacaag acagggtgcg acaaatttaa aacatcgact ttacaaaaaa
      841 attcaaaaaa tgctggttta atatctgcat aataactaat tttgtatgct tctttgctcg
      901 ttctcctcct tcccaaagaa atagattata agtccatggg aagaaggtag atttatgaat
      961 tctttaaaag aagatttcat tttagcaaag gctggaaatg aagaagctgt tgaggcaatt
     1021 ctcaaaagat ttagctcttt gattcataag caatcctgga gaactggaaa atatgatcaa
     1081 gattgttatc aagaatgtat gcttgcaata tatctagcta tctcgaaatt cgaaattaaa
     1141 gaataactca actaagggag tctgtgacat aagtaaaaag atagtttcaa atttcgaaca
     1201 ataaatttga aactatcttc tttataatta taaagtatcg attttacagc gttctataat
     1261 gattcataga atctaaaacg aatgatttta attatcaacg ttttttaatt cgtttttagc
     1321 ttaaaaataa cttttgtcac agtctcactc ctaaaattac tttatttacg tatgaagtgg
     1381 gcaggaggtc gaatccagaa ctgggctatg gtacttcaac gaggacttct cctctagaat
     1441 tgaaaagcat gcgatttacc ttccgtaacg acggtagatt tattcaacta tgtgtccaat
     1501 caaaatttgt ctgatttcaa aaatagtcaa gagaattttg gttgttaaaa actcttatct
     1561 atcaacttcc acagtttagt tgacaaaccc tgatactaag ttgggctatg atacactttt
     1621 cttgactatc agttacgtta ccggaaggaa tgaactctat tgagaaaaac atatgatcgt
     1681 aaatttaaac taaagatttc acaggatatc cttgagaaaa aagtaactac caagaaaatt
     1741 gctgaagaat acaatatctc tcgtccaaca atttcccgat gggtttcaga atatcgtcga
     1801 tacgggaaaa atgcttttgc tggacaagga aaaagattac ctgataaagc agatttctac
     1861 attcttgaac aggaaaataa acgactaaaa gaggaagacg agtgttctac gaacacaaag
     1921 atcgatacgg atctccaggg atcactcaaa aactttacga tgaaggaatc gaaatcaata
     1981 aacgtgttgt agctgttctt atgcgtgaaa tgaatttatg cacaagagga tttcatcgac
     2041 gcaaatcttc tcatggtaga gaaaaaacaa tcaaagaaat ggtaaaaaaa tgttgaatcg
     2101 ataatttgaa cagaaccaaa ttgatgctgt ttgggtgatg gatattactt acattccatg
     2161 taatgacggt cgcctctatt tgtccaccta tattgactta gcaactcgta ttctacgtta
     2221 ctatatggta gattcacaca tgaaaaaaga gattgtcgtc cgtccattac aaatttatta
     2281 aggagaattg cctacggtaa ttcattccga tagaggtagc cagcatcggt cttttattta
     2341 tcaagagctg ttgaacgacc accacatcac acattcagtg agccaaactg ggactcctgt
     2401 tgacaatgca gtaattgaag cgtttcatcg atcaatcaaa tgagaattaa tcgactccaa
     2461 caaatataaa agtaaaatag agatgaaggt gttaatccaa gattatttag aagactacta
     2521 cccaaacaaa agaatccata caaaatttat gatgactcct tgacaatttg aagcgaagaa
     2581 aataagggat ctataatatc tagtgttggt gacgccttct gttgccaata ctttttcttt
     2641 tttctaataa acgcaaacag acaccttaaa atcctttaga ataaagtcga cgaaaaccat
     2701 tcaaaggagt gtttcaagat gtcctattca gagtttacca aagagttact agatattcta
     2761 gacctaaatc ttatctttca tgaagatgcc tttcgcaaag agcgaatcaa tgacgaaaca
     2821 tgttttgttt ttgatggcac attgacctat caaccggagg aatgctttca tcgccactat
     2881 caaaatgaac aaacgatcat taaatagggc tggaaaaaag tatccatctt attgaatgat
     2941 gtcagtaact acaagaccat tctgcggatt aacaaacagc gcttcaaatg taaacactgt
     3001 ggaaagactt ttttagcaga agattcagtt agtgatcgtc gctgctccat tgctcgcaga
     3061 gtcaaacaag ccattcttga gctattaagc gaacctcttt ctatgtcttt gatcgcacgt
     3121 atgaagcaca tttctccgac cactgtcatt cgaatacttc gcagccttcg gcctaaaacc
     3181 atttctttga gccagctgtt accagaagtg gtgtgtttcg atgaattcaa atcagtgaaa
     3241 aatgtctcgg gagccatgag ctttgtcatg atggacggca agacccatca actgattgat
     3301 attgtggaaa atcggcaact caatccttta cgcgactatt tcttgcgcta ccctataaaa
     3361 gtccgtgaac aagtgcgtct agtggtttca gatttctata caccctatcg cacattggtg
     3421 aaagagtgtt ttccgaatgc ccgaatcgtc gccgatcgct ttcatattag ccaacacatt
     3481 ggtcgggctt ttaccaacca ccgaattcaa gtaatgaaga cctttaaaaa aggggatcgt
     3541 cggtcgaagc acttaaagaa atactggaaa ctacttcaaa agaatgcgtg ggaattgaaa
     3601 ggtcagcatc gctactggcg tccgtcattt cgagatcatc tgacagaagc agagatagtc
     3661 gatcggctat tatcttatga tgattcctta aagcgaggct acgaagtcta ccaggatttt
     3721 ctttccgtta tcagaagaca agatgttcct gaattcgtcg cgctcctaaa agaagactac
     3781 aaggagttgc cagagcacta ccaaccagtg ttcacgacct tcaagaaata ccaaacagaa
     3841 atcaaacgag cgttgcgcgt tccctattcc aacggcccta tagaatgttt gaataaccat
     3901 atcaaagtac taaaacgaat tgcctatggc tatagaaact ttcaaaacta ccgagaacga
     3961 atctttttat atcgaggaaa atattttaag aaaactaaga atactaccca actgactaaa
     4021 gccagaacaa caacacggct agacaagata gctgtctagc cgtgtgagta tctcgattat
     4081 tctattgaat ttcttcacca acactcattg acgaagagcc aaaataagta atctaaccga
     4141 ctgttaacta aaccattgca ggtcccgtta aagatacttg tgcctgttgt caccgcttct
     4201 ttttcttgtc cacaagcgct taacacactt aataaagccc ctgctattat taataataat
     4261 atctttttct tattaaaaac ctcctcatct atttccagat tagtaaatgt agtaatctgt
     4321 gctttctttt ttctcaatac taataactaa cttatggggt aaacaaatac tcgtttgacc
     4381 gggttttgag atccatccag ttctaaccgc gatttgatca ggactattat cttctttaac
     4441 tcgaattctt ccatctttaa tctcaacaat attatattgt cccttcgcag gatgataatt
     4501 cacgagttta tgattgatct tatccaaatc gaagcgatcc atttccttgc catcaattcg
     4561 aacgatggca tatttgatag catcttcagt tgtttcattc tccttttcta tagagaatac
     4621 aaataaggga ataaaagagg taataatcaa aatgccaata attatgacat cgcctatctt
     4681 gacaccagct tttcttaaaa atttcattaa cgatatctgg ctaaacatat tagacttttt
     4741 ccatggaaat ggtgaaattt ttcggaaatt atctgcatta agagaatcag ttaaacaacc
     4801 ctcaagttgt tccttaatac attgttgatc catcttcata ccaataaaaa ctagtttatt
     4861 aatgcgttca ccaaacaacg gatgccaggc ttccttcaat tcgggatttt tttgtataat
     4921 catttgacac tcaattttag gtaaactagc tacccaataa tttaatgatt caatactttt
     4981 atagctaccc gatgtttcaa agatcgtcga aaaatcacta tgttgggcaa accaaagaaa
     5041 cccttttgct cttgtaatat tgccaggaaa atttttatcc gtaagatggg caaatttttg
     5101 tgcgtcaagg agtaaccgat ttcgatagat aaaacttgag ataccatatt cttctgtttc
     5161 aggaatatga ttttgatagc caagctctaa ctctttgaac cagcccactg aataggaagt
     5221 ctcatctata ttaaaaagat ttgtatttag aattttcgat aagtctactt cacctttaat
     5281 tgtcctaatt attttggcat tggactgtaa cttagcaatt agaccgttaa ttttgtaaat
     5341 cgtcttggtt gacactaagt cactattatt taaaagtaat atgttacaac actcgaattg
     5401 atcaattaac aagccacgaa tatcctgttc ttcttcaact acattacctt cagcttttct
     5461 ttcctgaatc ttttcacccg tttcgaaatt atcccaaaaa cgttttacat ccactactat
     5521 aataatggca ccaatctcta atcgttgggt caaatcaatg tctaaagggt ttttagctaa
     5581 tataattgct tgaataattg gaattggttt gctgattcct gaagcttcaa taacaatttg
     5641 attcaagtca gaaaattgca ctaattgatc tagttcaaca atcaaatctt cgcgtaatgt
     5701 acaacaaatg catccattcg gcatcgaaac aagtttttct ccagttcttt taaaaccttt
     5761 cttgtcgact aataccccat caacatttac ttcccctatg tcatttacaa tgacagcaat
     5821 ttcctttccg tgatcaccgt acagaatatg atttagaagt atcgtttttc ctgaaccaag
     5881 atagccagtt aagattgtaa taggtatttt tttcataggg aacctctttc tataccttga
     5941 atttacactt taagtgcaac acataaaaat gagtcataat cgatatccaa ctagtataag
     6001 tttgtcgaga acactcctag aaggaaaata attatgagcc agtatcgtca tcttactata
     6061 ctcgaacgag aaagaatctt tctgcttcac gaagaaggat tttctattag tataattggc
     6121 aacgacattc agcatagccc gtttactatt ttacatgaac tgagaagaaa caagaaaaaa
     6181 actattcacc ttccaaagcg gaaaaaaatt atgcttatcg aagaaaaaaa tgcggacgtt
     6241 atcacattct aaatgatccg tgagtttggt caattgttcg tcatttattt gtcgattttc
     6301 agtggtcgcc agaggaaatc tcgaatcgtt tgaagcatga acatatggac ttttcaatta
     6361 gttttatcac gatctaacgt gcaatttgca atgatctttt cgatacagaa cccctctcac
     6421 acggcaataa aggcctgatc cgttcattgc accatcgagg aaaaacaaga catacaaaaa
     6481 atcatgtcga gtctcgtgga aagatttgcc ttactcattc gatccatggc cgtccaaaat
     6541 ccgcagatga tagaagtgat tttggtcatt ggaaagccga tattgtagct ggcaaaactg
     6601 gtggtacttg tttagtgact ttgacagaca gaaaatcatg cttcctttta gcaggaaaga
     6661 aggtccccag aaagaaagcc gcttatgtga gcgagaaaat gattgaactt ttaagcagta
     6721 ttccaaatga ggcggtcaaa acgatcactc cagatagagg aaaagaattc tccaatcatt
     6781 tcgcagttac agaagcattg aataatatgc ccttttattt tccagatcct catgcccttg
     6841 gcaacaatga acaaattaaa atacgaatag actcattcga gaatacttgc caaaatcgaa
     6901 agagatagct acggtagata actcaacgat aaatcagatg attgaaaaac tgaataacag
     6961 acctagaaag tgtttagatt ggagaacacc ttatgaagtc ttctttgata aagtgttgcg
     7021 cttgatttga caattcaaga cataataaaa agcagatcaa atccaaataa gggaaaaatg
     7081 atctactctg gtattatcaa ttaattggcc aagctattat gccgttttgc taaacgtcta
     7141 cttcttccag tattgacctt gataaaacaa caatcaggat cccgttttaa tactctatga
     7201 actgtgtcaa tactaacatg taaagttgca gcaatatcag ctaatgtact cccatttaat
     7261 ctcatttgct ttgcaaatcg ataacgccga taagcatcaa tattcttgga atccatagac
     7321 aataatactt ttcttaccat gtatttcctc caaaaattga attttaatga gccatcattt
     7381 cttgagcaat ttcatgttta gacaatgaag ctggatagaa agtaggccaa ttatgcaatt
     7441 cattatagag tttttcttga ctttctccac caaagaaaat atggaaatgc tctgccttac
     7501 ttggtgcaat tccatgatca ctaaattgaa tatatttaaa acgaccagca cctgcatcat
     7561 cagtttcaaa attaaaacga acaccacggt ttcctttttc ataattcaaa attttgtatc
     7621 caacatattt gtatgtgtat tgatgatgtt caccgttgac tacaaaatca atggtgttgt
     7681 ctttaatgtt gatagtatca atatctgtct tatagccagt agtataataa tctttgtact
     7741 cggcagctgt catgtcttta ttgatttttg ctttgtaatc gaaaacttca tccaaaattc
     7801 catcttttaa caacggataa acagattgcc attcgcctgt ataatcactc aattcgcgat
     7861 ccttcacatc ggcatcttta aagtacccat tagcaactgt cttttcatcc ttcgcttctt
     7921 tttctggaac tacctcttca ccagcaacca tagttgtttt ttctaacgct tttagattgt
     7981 cttccattac tgaaacataa tcttcaccat tatccatttg ttcttttgta agactttcaa
     8041 gaggattcag tacttccaat tttatcccag cttcgttggc caaagttttt gcaattttat
     8101 cattagcatt cttttcaaag taaatataat tgatcttatt ttttttaaca tactctttta
     8161 attcagctaa tcgaccagaa gatggctctt cttcaggact tagtccagca ataggaactt
     8221 gtttcaatcc ataatccaaa gctaaatatc caaaggctgc gtgttgcgtc acaaaacttt
     8281 tttgtttcgc ttctttaaga ctagtggtat agtcagcatc caaccgtttc agtttagtca
     8341 aatacttttc agcatttgtc tcaaatactt ttgctttttt cggatacaat ttaactaact
     8401 gatccttgat atttgaaact tccttaattg ccattttggg tgaaacccac gtatgaggat
     8461 ctagttcatg atggtgatct tcttctccgt gatcatgatc ttcttcttca ctacctggca
     8521 ataaaatcat gtcttttgtt ccttctacta cattaggccc agctttcttc caactttctt
     8581 tagcttttgg tacccatgat tccatgtttt cgttatgata tacaaaaaca tcagcatcat
     8641 gagcttttgc catatcctta gctgagggct catagtcatg aggttctgat cccgctggaa
     8701 ccattaaatc cacgttcgct tcatcgccga cgatgttttt ggtgaaatca tacattggat
     8761 agaatgttgt tacaatttct aattttccat ctttatcatt cgatactttt gcatcattag
     8821 tgctggagca agctcccaat aatcctagag ctgctatccc aataagtcct acactgattt
     8881 tcttcattct ttttccttcc ttaattatgc taatttcttc attttatttc gtttatgttc
     8941 tttgataatt tttaatgccc tttttctagt tttaatattc gggcttttaa gtcgtcgata
     9001 tgcactagat atatccgttt tctccattag aaattacccc caatcactac ttatcattat
     9061 ttgctttccg ccagtaattt atcaaaactc tgtttgatcc tttcttcatc caacattttt
     9121 ccgattagaa taatagtggt ttggtctttt tgtcgagtgg tgtattgaaa attaacctgt
     9181 tcgtttacct cttgcatggc aactataaaa tcctgctctt gtaaagcaat caatccttta
     9241 aaacgataca ggctcccttg gttggttaaa atcagccagt caatccattg gagtaaaaaa
     9301 tcttttttta tgtcttgatc tgaagttaag agaattgatt taaactcatg tacttgatgg
     9361 tgttgatgat agtatttgcc attatagtga ttttctgatg aatcactttc aaaaactaag
     9421 ggatgattaa attttttcaa attataaaaa tccttttttt gaatcgtttc actatgtgtg
     9481 aacattcgaa tatcggataa agggttaata cttttgattt ctctctgtaa tttttttagt
     9541 gcagataaat tcaagttttg ttttctagac acaaaaatgc gatctgccat gacaagttgc
     9601 ttaagggctt gtggttctct ttcaataagt tcaaaactgt cactatcaat aacagtcaat
     9661 aaactatcga tatatactct tcctcttagt tgaggcgctg ttaaaagtgt ttgaatgatt
     9721 ggttggggat ctgctatacc tgtagtttca atgatgattt gtgaaattgg ttgttgatgt
     9781 tcttctattg tttccagtaa agaaatcaat attgctaata aatcactgcg aagactgcaa
     9841 cacatacatc cattatttag ttgtaacacc cgttcctcgc tatgaactaa taatttgtga
     9901 tcaatcccaa cttcaccaaa ctcattttca attataataa tttcttccct ggggtagggt
     9961 gagacttgca aggcttgatt gattagcgtt gtttttccag cccctaaaaa cccagaaacg
    10021 attgtaactg gtatgcccat tttgtcacct ttttaccact tagctatttc acttctttaa
    10081 aaattgcccg tctgcgcaat tttggcgaat acttttttaa ttctaaccgt tcagggttat
    10141 tccgtttatt tttgctggtg agatacagac gttctccagt ttcgacacat tccaaaataa
    10201 tattttgacg cataaatctt cctccttcaa tacttatcga tcttcaagat atgcttgaat
    10261 ttgttgtgca tcttcaaaag ttaactcgcc agattccaat tgtgaaacta aattttcttt
    10321 catatcaaca ggtatctccc aataaccatc gatacattga aataactcct cgtcatactc
    10381 ttcaaaaatt ggatagattc ttaaaaaatc atcaaatgat aagccttggt cctcatagta
    10441 atctttgact aatccatttt caattgcctg ttgggtcggt aaacccgttt cagtaataat
    10501 tcctctctct accatcgaac gataaaaagt gcatttatct agcgattgct gggtcgtttc
    10561 aagataatac tttaatcctt cttcaactaa aggtgggtaa ataatttcca ttttgattcc
    10621 ctcctaaaag ttatatatta aactattttg aagctccttt aattactttc tttccattat
    10681 agtacccttt taaagataca tgatggctac gtctataatc accattcgct tcgtcaaatg
    10741 aaatttctgg ttttactaca gtttgatgag ttcttcttaa ccgtttcttc gccttagatg
    10801 tttttcttgc tggtactgcc atgatatttc ctccttttac caacttgctt tttttactcc
    10861 aggaatcaac ccttgatggg caagttctct aaacttaata cgggacatac cgaactttcg
    10921 catataccct cttggacgac catccgtctg atcacgaagc ctcagacgat tcggatttga
    10981 atctcttggc aatttagcga gagcggtgcg atctcctgcg gcttttaatt cctgacgttt
    11041 atctgcatac ttttcaatca tcgccatttg tttctttgct ttagcaattt tcgacttttt
    11101 tgccatatac ctttatactc cttttatttt gatttagaca cctcaaatcg taatcgttac
    11161 gttttgacac tgatacttta ccaacaagag ttgctcttgt caacaataaa taacatgaaa
    11221 taaaactcat atgaatagat aactatttga aaacgaattt atcggtacat agagataatc
    11281 aaatcattta aaagaatatg cagtttaggc tctgaaaaat aagaattcac ttttattagt
    11341 ccaatcaaac acaaataccg ttttaagtag cttttcctct acttaaaacg gcaatatgaa
    11401 agaaactata actttttatt caatctttta tgtttaactg gtttatcgcc aattttttaa
    11461 aactttgatt attccttata ttataagaat agacattaag tgaactaatt caatcatact
    11521 gattctttcg tgtattcaaa attagatgct gtttaataaa attattcttt ttgaaaaaga
    11581 attataacta tatcccaaaa attgtaggtt aatttaaagc ctatttcatc atcgtgattg
    11641 agcttctttt tcatagacga atttctcaga tagtttagtt ttctttatga gatttttgta
    11701 tatttctgac cttcgataca actcattatg agtaccttga tcaactagtt ttccttgatc
    11761 catcactaca attttattta ccttttcaat agacttcaag cgatgcgata taattagtac
    11821 tgtcttatct tgtattaaac gatttaaact ttcctgaata cgtttttcat tttccgcatc
    11881 taaactggca gtaatttcat ctaaaattaa aattggcgca tttttcaaaa aagcccgtgc
    11941 tatggatagg cgttgtcgct ctcctccaga aagtgaactt ccgttttctc cgaccaatgt
    12001 atgataaccg tcaggtagtt tttcaataaa gtcttcgcag ttagctaaac gagctgcttt
    12061 tttcacttcc tcatctgacg cttgactatt tccaatacgt atattttcca aaatacttgt
    12121 attaaacaaa gttacctctt gaaaaacaat agcgattttg ctaaacaaag aagctggaga
    12181 tacacgttta atatcatacc catcaattaa aatacagcct tcatcataat catacagtct
    12241 agacactaat ttcaagatac tagttttccc ggaaccacta gcacctacta acgcagtcac
    12301 ttctccttgt ttagccgtaa aagagatatg atctaaaatt ggggtattgt tatcatatga
    12361 gaaagagaca tctcgcaact caatgtcaaa agatttcaga ggactatctg atccctcttg
    12421 gatactggtt tccttcatag ctctaattcg ctgtatttta ggagctaaat aaaatatttc
    12481 aagcactgct tctttcatag aatcgaagga atcctttatt tttattgcgg ctagtaagta
    12541 accaacgacg tagagtatag tcacttcacc cgttaacagt aagtgaacac ctactagaat
    12601 gactactgct aaactaacat atgagaaaat agaggataag gccatgactg aaaaagtact
    12661 taattctacc tttaaatgaa tcctttcact ctcttccatt tttttgaaaa gtctgtcttg
    12721 aactttttta gatagattaa agctattaat ctcttgctgc aattctatag tttcttgaaa
    12781 ttcttccgag ttttctctca aagtatcgta atatttagta ttggctttcg tttgggattt
    12841 ctttgataac aagattaaga caaaactaaa taacgttggc aaaataacag ctaatcccat
    12901 tttgacatta ccaaccaaaa gcatcactga aataaaaggg aaaaacagag ccataccgcc
    12961 ggctttaggt attgcatgac tcatcgcatg ctcaatacct tcaacgtcag acatgatagt
    13021 ttgagataaa tctgataaat tatgttttga aaaataggat aatggtaaat ttgacaaatc
    13081 gtctgcaatt tgcactctta aatgcgcact ttctttataa gtactattat ataagttttc
    13141 atactcccta tctaacaacc aatacatgaa aagcaaagta acaaacgata cggcaaaata
    13201 aagccaacgg ggctttgtat tttctaaaac taattcgtct atcagcatca ttataatgaa
    13261 tgctggtacc atattaatgc agtatacaaa aaaactagaa atagaagctt tagttaaagc
    13321 ttctgcgccc tgatcactta aagcaaattt gtttttataa aaacttttca ttgtcaagcc
    13381 tccaatcatt tgcattctca taattagtga ccaattgttt atacacttga tgtttagcca
    13441 ttaattcatt atgatttcca cgttctacta ttttcccttc tgacaagaca agaatttcat
    13501 ctacagattt aatgctggat aatcgatgag ctatcataat aactgttttt tctttcatca
    13561 gatgttgaaa agccttttgt agttcatact cattatctgg atcaatagat gcactggctt
    13621 catccataat aacaatcttt gcatctttta aaattgctct ggcaatagct atacgttgtt
    13681 tttctcctcc tgacagataa actcctttac taccaattaa ggtatgctct ctttcgggaa
    13741 atttttctaa aatcgattcg caaccggcta atcttagcgc gtcaaatact ttacttgctg
    13801 gcgcttgagg atttgctata gaaacgttat cgtaaatact ctgattgaat agtttcgtat
    13861 cttgaaagac aaaagcaatt gtgttaataa ttgtgtcttt tgaatagtga tttaaaggct
    13921 taccaccaat tttaatctgt cccttatcca cattgtagaa acctgaaatt agtttagcta
    13981 tcgtactctt tccgcttcct gattttccta ctaatgcata aattcttttt tgttctaaaa
    14041 ctaagttgaa attttctaaa acacactctt ctttgtatga aaagctgaca ttttcaaatg
    14101 caatgtcaca atttttaatc tgtgtctcgt tcccatattt tagttggtct tgcttcattt
    14161 ctaaataaag ggagtctagt gtttcgactg catatcttgc ttcaaacaag tgcatactta
    14221 catacattat tctcatgaaa gaaacaaaaa taattccact taaaaaaaac aacatcaaca
    14281 agtcaacagc taatatcttg ggagtggtaa gagaagttat aaaaaaggtt ataggaacta
    14341 tcaaaatgca aactatgcca aaaaatagcc attgaaaaag aacaaaaggt gttttcccac
    14401 tcaaagaata cttatatgca aacttagcat aatcgttaat tgctttattc aaggctttaa
    14461 atgatgttac tttagcacca aatatcttta taacttgaat tccgcggata tattctaccg
    14521 tttcagagct taacacatct aacgattctt gatatttttg aataaaattt gtattctctg
    14581 tcattttctt taacaagaat acaccaatta ctaacatggc gattagaacc aatccaattt
    14641 taaagttaat gaaaaagcct aaggcaactg ataaaacagg tgttactatg gcttgagcac
    14701 tatctggaat caagtgagca attgccatat gtgttttagc cgcgttgtca tcaattgtct
    14761 ttcgaatata tcccgaagaa tttaaatcaa aaaaacgaaa acttgcttct gttaaaccgc
    14821 taattccctt ttttctcaaa ttagtctcta agcgaaaccc aaggatatga gaaaataagc
    14881 cagaaaaaat ataaaataaa gctcctaaag ttaaccaagc aacagtttga accgcataac
    14941 tcttagctaa atattcatct cctacaataa ttagagactg aagaaatttg taaatactgt
    15001 aatatccata gactgttaat acgacagaaa aatcagaaaa taaagtggct aaccatccgt
    15061 agtatttcat tttagggaca taactaaata attttttgta tactttcatt ttttcctccc
    15121 ccgcattcaa tttcttctct tattcgttta caactgttaa tgctttccgt aatagcgtaa
    15181 attagagatg tacaaacaga taaaaggtaa actacgttct attttttgta tttttttaag
    15241 acttttcttc caatcaatac accaatcata gataaacttg ctacaactaa cactgaaatg
    15301 attccccata tacctaccgc atagtggatc attgcatcca ttgttgcagc agattgtcct
    15361 ttatctatcc aatcttgacg aaaacgatct gtaaagaacc atataggaat ccactgtccg
    15421 gcagcactgc ctaattgcat tagagcccaa gacgcaactt gaggaattgc tcgatcatat
    15481 ccaaatacag atagaataat atcagctaag atcgccatac taatcgaact gataaaccaa
    15541 ggtaagtacg ccccgcccat taacgtaaaa aataacaata aaacaattgt gtaaatagta
    15601 aaaattcctt ttctagggta gttatagcta atataaacaa atatgattcc agataataat
    15661 ccaaaaatcc cgggggatat gctgtgacca aacgccccac caatactact aaccattatt
    15721 gaaaaaaagt aaattactag ggaaataact gtagctaaac caataaaaat ataatctttt
    15781 gttttcatat atattctcct caattctatt tcgttatgtc attaaaaatc tgttctatca
    15841 aagttgaatc ttctactcta agttttatat ctttttttat tccaccattt tttaaataaa
    15901 taattctatt gcatgtttta taaagaaatt ccggatcatg agtagctata actactgcac
    15961 ccttttcttt taagtcttta ataaggtcag caatcctttc catattaata taatccaacc
    16021 cacttgtagg ttcatcaaat agaaataaat tagtttttga caatgaagct atagaaacca
    16081 ataatctttg tttttctcca ccggataaat caaacggtaa ttgatctttc attttataaa
    16141 gttccatttt ttttaaaatt ttttcttgct gatctttact aactatttca ttccccaggg
    16201 ctaactcgct cctcactgat tctgtgaaaa attggtaatc catttcttgc attactaaaa
    16261 acggcgatga caaatttttt ttattttcga ttatctttcc tttggtaggg cgaataatct
    16321 tcatgagtat tcttagtaag gttgttttcc ccacaccgtt tttccctacc aaaccaataa
    16381 catctccaac tttgacatca aaatttatgt cttttagaat attttcataa gtaatttctt
    16441 caacttttaa tacatctgca cttacctttt cgatataggg aacttcaatc gaactattaa
    16501 aaatgtcaaa tttccttaat gcgttattgt tttcagtttt taacaactga atagttgtag
    16561 tttttacagt tctattttta acaaatagaa cttgatcaat taattctttc aaatagtaaa
    16621 aacgatgttc agctataaca atagtaaatc ctttattttt taaatcacaa agtaagtcag
    16681 caagtttctt agtcgctttg aagtccaaat ttgaagacgg ttcatccaat ataattaatt
    16741 tagagtttag cgataaacta catccaatag aaattttctg ccgttcacca cttgatagcg
    16801 aaaaaatgtt tttatctaat atatttgaaa gatctaataa ttcagctaat tcattgattc
    16861 ttttatccat atgctctttt gtaaatccaa agttttccat cgaaaatact aactcactcg
    16921 ttgtatttgt tgtaaagaat tgtcctctag gatcttgact tacgtagcca atattttttg
    16981 atatactttc ttgtgttaag gattgtagcg agttattttc aaataaaatt tccccaacca
    17041 attttccgtc ataaagtctt ggaataagtc cattcattaa ctttaatagt gtggatttcc
    17101 cactcccaga agaaccagta atcaatgtaa gactgccttt ttttattgaa aaattaaggt
    17161 caacaagcgc ttgttcctta acatccttat atgaaaaaga aacattgtta aattcaaaca
    17221 attctttcac ctcaaatcaa aatacttaag aaaaaaatag taacggaaaa caggatgata
    17281 tagtctaata agctcatgtt tgttttatag taagaacttc ttttcacagg agattctatc
    17341 ccctttgtta ttgcattagc ggtcatttct tcagcaatca tagttgcacg tataagaacg
    17401 ggaactaaca gaaattccaa atattgcaga ggctgaaaaa aagacaagcg aatgtttctc
    17461 atttttaaag attctttaat cattcttatt tcagaacgat aagtcggaaa gaatctgatc
    17521 actactgtaa tacttaac
//
LOCUS       DACECZ010000047        17408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.047, whole genome shotgun sequence.
ACCESSION   DACECZ010000047 DACECZ010000000
VERSION     DACECZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.047"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(24..635)
                     /gene="rpsD"
                     /locus_tag="IWO35_001485"
     CDS             complement(24..635)
                     /gene="rpsD"
                     /locus_tag="IWO35_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ0316546.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            951..1748
                     /locus_tag="IWO35_001486"
     CDS             951..1748
                     /locus_tag="IWO35_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ0316547.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            1776..2411
                     /locus_tag="IWO35_001487"
     CDS             1776..2411
                     /locus_tag="IWO35_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ0316548.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            2431..3204
                     /locus_tag="IWO35_001488"
     CDS             2431..3204
                     /locus_tag="IWO35_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0316549.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            3612..4409
                     /locus_tag="IWO35_001489"
     CDS             3612..4409
                     /locus_tag="IWO35_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ0316550.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            4387..4800
                     /locus_tag="IWO35_001490"
     CDS             4387..4800
                     /locus_tag="IWO35_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316551.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            4784..7429
                     /gene="mutS"
                     /locus_tag="IWO35_001491"
     CDS             4784..7429
                     /gene="mutS"
                     /locus_tag="IWO35_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0316552.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            7447..9555
                     /gene="mutL"
                     /locus_tag="IWO35_001492"
     CDS             7447..9555
                     /gene="mutL"
                     /locus_tag="IWO35_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ0316553.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            9577..10137
                     /locus_tag="IWO35_001493"
     CDS             9577..10137
                     /locus_tag="IWO35_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ0316554.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(10222..12210)
                     /gene="treP"
                     /locus_tag="IWO35_001494"
     CDS             complement(10222..12210)
                     /gene="treP"
                     /locus_tag="IWO35_001494"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ0316555.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            12433..13146
                     /gene="treR"
                     /locus_tag="IWO35_001495"
     CDS             12433..13146
                     /gene="treR"
                     /locus_tag="IWO35_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ0316556.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            13223..13669
                     /gene="msrB"
                     /locus_tag="IWO35_001496"
     CDS             13223..13669
                     /gene="msrB"
                     /locus_tag="IWO35_001496"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ0316557.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            13839..14441
                     /gene="ruvA"
                     /locus_tag="IWO35_001497"
     CDS             13839..14441
                     /gene="ruvA"
                     /locus_tag="IWO35_001497"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ0316558.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            14454..15455
                     /gene="ruvB"
                     /locus_tag="IWO35_001498"
     CDS             14454..15455
                     /gene="ruvB"
                     /locus_tag="IWO35_001498"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ0316559.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            15484..16062
                     /locus_tag="IWO35_001499"
     CDS             15484..16062
                     /locus_tag="IWO35_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0316560.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            16114..16596
                     /locus_tag="IWO35_001500"
     CDS             16114..16596
                     /locus_tag="IWO35_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ0316561.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            16713..17087
                     /locus_tag="IWO35_001501"
     CDS             16713..17087
                     /locus_tag="IWO35_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316562.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         5773 a         3108 c         3814 g         4713 t
ORIGIN      
        1 ctttttcaag aaagcttttg aaattataag ctttggttgt agtattcaac gacaagagct
       61 tcgtcgattt ctggatatag ttcgtcacgt tctggtaaac gagtcaatga accttccaat
      121 ttttcagcgt caaagcttac gaatgctggg cgtccaacag ttgcttctac agcttctttt
      181 actgttacga tgttttgtga tttttcacga actgagatca cttgacccac ttctacgtgg
      241 tatgaaggga tatcaacgcg tttgccatca actaaaatgt gaccatggtt aactaattga
      301 cgtgcttgac gacgagtcgt tgcaagtcct aaacggtaaa caacattgtc taaacgttgt
      361 tctagtaaga tcatgaagtt aacaccatgt ttaccttctt tgattttgct agcttttacg
      421 aacaagttac ggaattgacg ttcgttcatt ccgtacatgt gacgaagttt ttgtttttca
      481 gttaattgca tgccgtattc tgattgtttg ccacggctat taggtccatg ttgacctggt
      541 gcgtaagggc gacgtgctaa ttcttttcct gtgcctgaca atgagatgcc taagcgacga
      601 gaaattttcc atgatggtcc tgtataacga gacattaaaa tttcctccaa tgaatataag
      661 tttttggagt aaaataatcc gttgaaaaat tcataatcat atagtttgtt cttcaatctt
      721 cacctttgca gccgcggtta cacaatcgaa cccgagacgg ggcaacaaac tagggatgag
      781 ttattatttt tctgctgcat tattttacac aaattacagt atatgataga tcatccacga
      841 atgtcaagat aattagtaca taaaaacgct ttacaaacct aggctgtctt tataaaatga
      901 gagggtatgt gcaaatggaa aaaagcgaag gaagtttaag gagaaaatca atgaatccgg
      961 tatcaccatt atttgagaaa atcgatgctt caattgaaaa gaaggcgaat ctttttgaaa
     1021 acagcttttc acgttacgct gttcgagcgg ttctcgcctg tttgtttttg actttaggaa
     1081 cggctgtggc atttgcgatc gcgatgaagg gggaagatat cgcgcatggt ctaggaaaga
     1141 ttttatacgc atttatgttc agctggggtc tggtcatgat tctatacatg aacgccgaat
     1201 taggaacatc gaatatgctt tatatgaccg tgggtgtttt tcggcagaga cttgctttaa
     1261 gtaaggcagc caagattcta tttgcctgta tttttttcaa tatgattggc ggtatattat
     1321 gtggatttct aatttctatg actacgccat ttcaagattt aacgttggac aactttatgt
     1381 tagaatcaat agcagtaaaa ctagcaaaac cttcagcaac gattttggtg gaagggatgt
     1441 ttgcgaatat cgttgtcaat accgctgtac tgatcagcat gcggatgaaa gaggatgtcg
     1501 gaaaagtgat cacagtcgta ttcatcattt tcatttttgc ttttttagga tatgaacacg
     1561 tgatagcgaa ttttcctgcc tttacgctta cttactttgc ttctcacgga cagatggatg
     1621 gaatgacagt cagcaatgtg ttacataatt tgttttttgc tttggcagga aattatattg
     1681 gtggcggttt agtaatggga ctgggatatg cttggctgaa tcagtcaaaa tcatcctatg
     1741 tggactaaaa agtcgttcat taaaggggaa gtgccatgga agttttagtt tttccaaaaa
     1801 atgaggaaaa agcgttacaa atggaaaagt acatgaaaaa tcttttttcc tttgctggag
     1861 tacctaagcc ggaacgcgct cttttacaac aaccgttact ggatgagagc aaaaatttat
     1921 ctatttctga agttttagct tttgttcatt attattatga acaaatcgaa agagagtatc
     1981 agtatgtagc gatcgattta gtccagaaaa acatcaaacg gcttacattt gaagacttag
     2041 tcgtattgtt gccactagtt cagaaaaaag aatggtggga cagcatcgac tcttggagaa
     2101 aagtatacag cacatggtgt aaagcctacc cagagcagcg tgaagaagtc tttcaattgt
     2161 ttgaaaaaaa ggaagatttt tggctacgtc gaatagcaat cacgttgcaa ttaggttata
     2221 aacagcaaac agacacagga ctattgatcc gagcaataga cgaagatcgt caaacaaagg
     2281 aattctttat ccaaaaagca attggttggg cattacgcga ttatagtaag accgatgccg
     2341 attgggtaaa aagacagctg acaacagatt tgagtaagtt agctgttcga gaggcaagta
     2401 agtatttata agaaagcgag acgacaagag atgagacgca aattatttgc ttttgatatt
     2461 gatggaacgt tgttagattc aaacaaacaa gctttagata gcacaagaga agcattggaa
     2521 aaattacgca aagccggtca tctggtcacg attgctacag ggcggagccg atttcatgcg
     2581 caagagatca ttcgcgatct tgattttacg aactacattt tatgtaatgg agcggccggt
     2641 tttttagacc atgaacaagt atataagaat ttactagatc gcgaccagtt ggatgcgttc
     2701 gttcaagaag cgcatcatga gcagatcgat acagcttttg tcagcttaga caatatcaag
     2761 cgttcttctt ccaatgatgt aacggtgatg gaagaagcga tgcattcttt cggtgctgtc
     2821 ttgcctgaat tagatcagtt ttttgcagag gaacaagaaa tataccaagc gttagcattt
     2881 ttcgatcaaa catatgatgg gaagttctct tcatatgata aaattcgatt tgtccgctgg
     2941 catgagaaca gtgtagatgt cgtacctaaa aatggttcta aagcagcgac aattctgcac
     3001 ttagcagaac aagtaggcat ccctcatgaa gacatcatca gctttggcga tggacaaaac
     3061 gaccgtgaaa tgttgcgcat gtcaggaatt gggatagcta tggggaatgc tgtgccagaa
     3121 gtacaagcag aagctaagat ggtcactgat acaaacgatc aggatggaat ttggaaggca
     3181 ttgaaagagt taagcgtgat ttaaaagagg ttgtgaaaaa gctgttctgc atcaagtaat
     3241 aagcccagcc aaacaaaaat agctcctcct atttttcgtt tttctgggct tattaccgca
     3301 gatgcaagct ttcgaacacc gtttaaggaa gaggtcgtga cagaagtggt cagtttcaag
     3361 aaataagaag ggatttacga aaattgcttc tcaaattttt gtaaaattac ggcttatttc
     3421 tgaagaagta gatgaaacaa cgtttattcg ggaaatggga ctgtgacaag atgtttgtca
     3481 tagtcccatt tttttatttt atttttcaaa actaagaaat accaggaaag agttcctttt
     3541 gtttgacctt ttctttctta ttgaaaacaa ctataatata gagtgatgtt aaaagagaag
     3601 aggaatttag tgtgcgggta ttgtttattg gtgatgtagt aggttcaatg ggacgagaga
     3661 tgattactga atatcttcct agattgaaga agaagtaccg tccgcaagta acgattgtaa
     3721 atggtgagaa tgccgcttct ggacgtggaa tcacagaaaa aatctataaa aaatttttgc
     3781 aagacggcgt agatgtcgtg acgatgggga atcatacgtg ggacaaccgc ggaatctttg
     3841 agtttgtaaa tgaagctaaa aagatggttc gtccggcaaa ttttccggaa gggacgcctg
     3901 gacaagggat ggtctttgtc aaagtgaacc aagtcgaact ggcagtgatc aacatgcagg
     3961 ctcgttcttt tatggtggac ttggatgatc cttttcgtaa aatgaatgag ctggtagaag
     4021 aagccagaaa acgtacgccg atcatttttg tagattttca tggagaaaca acaagtgaaa
     4081 aacaagcaat gggctggttt ttagatggga aagtttcagc agtggtcggt acacatacac
     4141 acgttcagac aaatgatgcg cgaattttgc caagaggaac agcgtatctg acagacgttg
     4201 ggatgacagg tccctatgat ggtattttgg gtatgcgcag agaacctgta atcgaaaagt
     4261 ttctgactgc attaccaaaa cgttttgaag ttgtggaaca aggccgatcg attttaagcg
     4321 gctgtatctt ggaattggat gatacgacag gtcatgcaaa agaaatccag ctgattcaga
     4381 tcaacgatga tcgaccgttc atggagtaga aagagaggca gttttttgca gcaggagccg
     4441 gaaattcaaa aagaagtaag gaaactgaca aaacttttaa gagagaatga aacgatcatt
     4501 cgctacaaag aacttgaaga aaagatccag caaaaccagt atttagctga gttgagagaa
     4561 aaaatcaaac aagcgcaaaa agatgctgtt cattttgccc actatgacaa accagcagca
     4621 gaaaaagaag caatcaaaca agcggatcaa ttcatgcaag aatttgatca gcatccgctt
     4681 gttgtagctt atagaaaaca gctgttggaa gcagatgatc tgcttcatca tttaacaact
     4741 atgatacaag aagaaatcaa tggacaaata gaggaggaaa aacatgcctc aaaaaactaa
     4801 aaatactcct atgatggaac aatatctaag catcaaggcc caataccaag atgctttttt
     4861 gttctatcgt ttaggagact tttatgaact attttacgaa gatgcgatca aagcgtcaca
     4921 gcttttggaa ttaacgttga cgagcagaaa tcgaaatgca gaagaaccta ttcctatgtg
     4981 cggtgtacct caccatgcag cacaaggata tatcgatgcc ttgattgaaa aaggctataa
     5041 agtagcgatt tgcgagcagg tagaagatcc aaagacgact aaagggatgg tcaagcgcga
     5101 agtgatccag ctagtcactc ctggtacagt catggacagc aaaggattgt cggcaaaaga
     5161 caacaatttt ttaacagctg tagttagtca aggaaatgaa tttggctttg cttatgccga
     5221 tttaagtacg ggagaattga agacggctcg gttgcatgat gaagaagctg ttttaaatga
     5281 agcctctgca ttgcagacaa aagaactcgt attaggcagt gagatcactg atacccttcg
     5341 tgaacagcta agcgggcgtt tagggctagt cttttctcaa caaaatgaga tggaagaaaa
     5401 cgcggaattt agctttttga caagcgaact agtcgatccg ctggaaaaag aagcaaccgg
     5461 caaactattg acttatttat cggtgacaca aaagcggagt ttagctcata tccaaaaagc
     5521 agtagaatac caaccagatc attttttgaa aatggattat tattcaaaat tcaacctaga
     5581 attaagccaa tctattcgga ctgggaaaaa gcacggcact cttctatggt tgttagatga
     5641 aacaaaaaca gcaatgggtg gccgtttact aaaacaatgg ctagaccgtc cgcttatcca
     5701 ggcaaaacaa attaaagcaa gacaagaaat ggtcgcttct ttgctagaca gctattttga
     5761 acgtatcgat ttgcagtcag cattaacgaa agtctatgat ctggaacgcc tagcaggtcg
     5821 agtagctttc ggtaatgtca atgggcgaga cttgatccag ctgaaaactt ctttagaaca
     5881 agtgccaatg atcagagggt tgattgaagg aatcgatcaa ggtcaatggc aaagtctgct
     5941 ttcagaaatg cagccgatgg accatctcgt ccaattgatc gaccaagcta tccaagatga
     6001 tccgccgtta caaataacgg aaggaaacat catcaaagat ggattcaatg agcagctcga
     6061 tatgtaccgt tctgctatga gaaacggaaa gaaatggttg gcagaacttg aagcaaaaga
     6121 gcgtcaagaa acaggaatca agaacttaaa aatcggattt aaccgcgttt tcggttatta
     6181 tatagaaatt acaaaagcaa atcttggaaa tgcagaactg gagaagtatg aacgaaaaca
     6241 aactttagca aatgcagagc gtttcatcac accagaacta aaagaattgg agacacaaat
     6301 cctcgaagca gaagaaaaat ctgttgattt agaatatcag ttgtttttag ctgtgagaga
     6361 agaagtcaaa aaggctatcc agccgctaca agtacttgca aaagcaatca gtgcagtaga
     6421 tgtgctacaa agttttgcaa cgatcagcga acgctatcag tatgttcggc cagaacttgt
     6481 cagtgacaag catcagcttc tgatcaaaga cggacgacat cctgtagtag aaaaagtatt
     6541 aggacatcag gaatatatcc ctaacagcgt agaaatggca gaagacgaaa tgatcttgct
     6601 gattacagga ccgaatatgt ctggtaaaag tacgtatatg cgacagttgg cattaactgt
     6661 tctgatggcg caaatgggct gtttcgttcc ggcaaaacaa gcagttttac ctatttttga
     6721 tcggattttt acccgaattg gtgcaagtga cgacttaatt gctggacaaa gtacttttat
     6781 ggtagagatg atggaagcca atcaagccct gcgttatgct acgccaaaca gtttgatttt
     6841 gtttgacgaa ctagggcgag gaacagcaac ctatgacgga atggcgttgg cccaagcaat
     6901 catcgaatat atccatcgtc atgtacaagc aaaaacactc ttttctactc attatcatga
     6961 actgacggta cttgagaaag aacttccgca gcttaaaaat gtccatgttg gagccgttga
     7021 aaaagacgga gaagttgtct tcttgcataa aatgatggat ggacctgcgg ataaaagcta
     7081 cggcatacat gtggcaaaga tcgcaggatt gccttccaat ctactagaac gagcagcaga
     7141 tatcctgcat acgctagaaa atggcgataa cggacaccat actgttccag ctgaagtaga
     7201 agagaaaact aaaacagcca aacaggagca aataaaacca cctgcagaaa aagtaaatca
     7261 agaccatata caagaccgtg taaaagaaga aaaccaacag ctttctttat tcagtgaatt
     7321 atccgaaaat gaaacagaag tcattgaatc actgaagcaa ataaatttac tagaaatgac
     7381 gccaatggat gcactaaaca cattatatga attacaaaaa caactgtagg aagaaggcgg
     7441 atatcgatgg ccaaaatcca agaattatct gaacgtttag ctaatcagat cgctgcggga
     7501 gaagtagtgg agcgaccagc ttccgttgtg aaagaattag tggaaaatgc aattgacgcc
     7561 ggcagtactc agattgatat cttagttgaa gaagcgggac tgcggacgat ccaagtgatt
     7621 gataacggag aaggcatttt ggcagacgac gtagagaatg ccttcaagcg gcatgcgaca
     7681 agtaagatcc ataatcgcga cgatcttttt cgcatacgaa cgctgggctt tcgaggggaa
     7741 gctttgccaa gtattgcctc tgtttctgaa atgactctgg aaacggcgaa tcaggaagaa
     7801 aaacaaggca cctatctgtc gctgaaaggc ggagaagtta ttgagcatcg accagcacca
     7861 ctgcgtcaag gaacgaagat taccgtcagc aatctgtttt tcaatacgcc agcccgcttg
     7921 aaatacgtga aaacactgca cacagaatta gcgaacatcg gggacatcgt caatcgactg
     7981 gcattgagtc atccatcgat tgcttttcgt ttagtacatg atggaaacaa aatgctggca
     8041 actgctggaa atggcgatct gaaacaaaca attgcgggaa tctacggatt atctaccgca
     8101 aaaaaaatgc tgaaaatcga aggaaaagat ctagattttg agttatttgg ctatgtttca
     8161 ttgcctgagg tgactcgtgc cagtaggaat tacttatcga cgatcatcaa tggacgtttt
     8221 atcaagaact tttccttgaa taaagcaatc gttgcaggtt atgggtcaaa attaatggtc
     8281 ggtcgtttcc ctattgctgt attggaaatc aaaatggacc cgcttttagt agatgtaaat
     8341 gttcatccga cgaagcagga agtccggctg tcgaaggaaa aagaactaac agagctaatt
     8401 agtaacgcca ttcaaaaagc attgtctcaa gaaaatctga ttccaagtgc agcggataat
     8461 ctacggttta agaaaaaagt acctgatcag cctaaaacag aacaaatcga aatcgatttg
     8521 tcctacgctt ttgaagaaga aaaacctaaa aagccaagta gtttgaattt tgatcgagag
     8581 acaggtacct ttttcgtaga agaactaaag aacacatttg ttgacagaat tgttgataag
     8641 tctgtggaaa atgttgaaaa ctattctggt ttttctgaag aaaacaccac ttatccagaa
     8701 aaaacagcga aaacggtgga taagtttggt gaaaatgttg ataactttgt agaaaaaaca
     8761 gtggaaaaat ctgaaaagaa agaatttccc attgtggata acatggagaa aacaaatgaa
     8821 gaacaagcag ataaatccgt agaagaggat gaaaagattt acgaggaaga attgacactc
     8881 catcctgaat ttgatttcac tcaaggaaac gacaaaacat tagaacaagc catcaagaaa
     8941 ttagaaacgg aaaaaccagt acaacgtttt cctcagttgg aatattttgg acagatgcac
     9001 ggaacgtatc tttttgccca aagtagcgat gggttgtaca tcattgatca gcatgctgcc
     9061 caagagcgca ttaagtatga atatttccgg gaaaaaattg gggaagtatc tgatgatctg
     9121 caagaattgc ttgttccatt tgtgttagac tatccaaaca gtgatgcttt gaaattaaaa
     9181 gagcaaaaac acttgctaga agatgcaggg atttatttag aagaatttgg tcaaaacagc
     9241 tttattgtac gggcccatcc tacttggtat cccaatggag aagaagaatc gattattcgg
     9301 gagatgatcg atatgctgct gacaactgga agtgtgagtg taaaaaaatt ccgtgaagca
     9361 acagcaatca tgatgagttg caaacgatcg attaaagcca accattattt gaatgaagca
     9421 caagcacgtg tgctgctgaa ggatctggca cgatgcgaaa atcctttcaa ttgtccccat
     9481 ggccgtccag tattgattca ctttaccaat tcggatatgg aaaggatgtt caaacgaatc
     9541 caagacccgc attaagtcaa agagaagagg gaaaacatga agattgtatt agcatcccaa
     9601 tcaccaagaa gaaaagagtt attggctcat atcgtttcag aattcgagat tgaacctgct
     9661 gatatcgatg aaacgccatt tccagacgaa cagcccgtcg attatgttcg tagaatggct
     9721 gaagaaaaag cacgaactgt ctgggaacaa gatgaaaaag aagaccattt agtgattgcc
     9781 agtgacacga ctgttgttct caatcaagag atcatgggta aaccagaaga tctggctgag
     9841 gctgaattca tgctgaaaaa actaagcggg gaaacacata ctgtatatac agctgttgtt
     9901 ttaaaaacaa aggaacgaga agaacggata ctagcagaag cacatgtcac tttttatcct
     9961 ttgacagacg aagagatcaa acgctacttg gagacggggg attatgcgga taaggcaggg
    10021 gcatacggca tccaaaatgc tgcgagtgta ttcgtcaaag aaatttctgg agattattac
    10081 agtatcgtag gatttccgat tggtgctgtc aatcaggcac tgaaaacatt catttaaaca
    10141 aaaaagcgga ctatctgatt ttcgattgaa aatccatgat agcccgtttt ttttattgaa
    10201 aagattactt atctactctg actaagattt tacttcagct ttaccgactg cttcgcccgg
    10261 gcgaacaagt cctgattggt caagagaaag tcctgcaact ttttcattat tggtcaaagc
    10321 aacaataatg tcagttttct tgccagcttg ttctatttct ggcaaatcca tttcagcaat
    10381 cagggtctct ggtgtgacaa ggtatccttc tttcacgaat acattgaaag gaacaccctt
    10441 tagttcaaca gtatctaatc ccatgtgaac aagtacttcc agcccctcat tagaaagaat
    10501 accaatggca tgttttgttt caaagacact cgtcacttta ccgtttactg gtgcatagac
    10561 tttgccgttt gacggttcga cagcataacc gtcacccatc attttttgtg agaaaactgg
    10621 gtcatttact tcagttattt ccttgatggt ccctgcagcg actgcgaata atgtttcttt
    10681 tgttgcaact gccgtaccag ttgctgaaac aggttcgaaa gactgtggcg ttgctgttgc
    10741 gccatttgct gtttgaagtt gtacatcagc agcattttca ggaacagcag gatcaatctt
    10801 attcaaaatt ccctgacgtc taaagatgat cgtcaagcca aatggaatga tgacagcaat
    10861 gatcattgcg atgatgaaag gtacccatgt ttctgcttgg atagctagta ttcctggcaa
    10921 accgcctact ccgatcgcgt tagctcgaac acccatcaag ttagcaaaca tgccagctaa
    10981 tccagagccg accattgcag cgacgaaagg atagacgtat ttcaaattga ttccgaacat
    11041 agcaggttca gttacgccta agtaacaaga aatcattgct ggaatcgaga tttgttcttc
    11101 ttttttattc ccacgatgta agaagatgat ggctaagact gcagagcctt gggcgatatt
    11161 tgataaagca atcatcggcc ataaattcgt tgatccgaaa tctgcaatca gctgcatatc
    11221 gatggcattg gtcatatggt ggagaccagt aatcactaaa ggagcataca ggaatccgaa
    11281 tacggcacta aacaaccagc tgatagaaga cgtcaatcca gtatttacga tatttgagat
    11341 ccagcttccg actgtccagc cgataggacc taaaataacg tgtgcagcta aaacagttgg
    11401 taacaatgaa aacaatggaa caaaaatcat cgagatcgac tgagggatat atttgcggaa
    11461 gaagatttct agatacgcta acataaaccc tgccagcatg gctggaataa cttgcgcttg
    11521 ataaccaatc atctggactt gagcaaaacc aaaatcccaa aatggaatat ctgctgcggc
    11581 tgtggaagct acactataag catttaataa ttgcggagaa actaacgtaa ttcctaaaac
    11641 gattcctaat atttgtgtag tacccatctt ttttgcaata ctccaagtga tacctactgg
    11701 aagaaaatgg aaaattgctt caccgggtaa ccacaaaaag gcattgacac cattccaaaa
    11761 cttcgagtgt tcaacgattg ttccaccaag actttcaaac tggatacctt caagtacatt
    11821 tctgaatcca aggatcaatc caccgacaat gattgcagga atcagtggtg taaagatttc
    11881 tgctaaaacg gcaatagcac gttgaacggg atgaaggttt tgttttgcag ctgcttttcc
    11941 ttgttctttg gaaacccctt caactccaga tactgcaacg aagtcgttat aaaaagtaga
    12001 aacatcgttt cctatgatga cttggaactg tcctgcattc gtgaacatcc cttttacgct
    12061 aggtatatct tcgatcgctt tttcgtccgc ttttcctgga tcattcagga caaaacgcat
    12121 acgagtcgca caatgactaa ctgcagcaat attttctttt ccgccgatat catgtaacag
    12181 cttctcggca tctgcttgat attttgccat atcaataaac ccctttctct taacttgtct
    12241 atgcaagttg tttcttcatt tactatactt tttttcggag agaattgcaa gcgttttatt
    12301 aaaaagtacg agagaaaatg atatcgtttt aaaaagctta aaataagctt ttcttaaaaa
    12361 taggaacatt gaaatacaaa aagagctgta gtatagtgaa gaaatgatgg aagaacgagt
    12421 gagggaaaaa acatgaataa gttcaatgaa atttttttag atttggaaca gaaaatttta
    12481 gcgaaagaat atcctccgca ttcgctttta cccagtgaga accaattgat caaaatttat
    12541 ggtgtttcac gagaaacaat tagaaaagcg ctgaatcttt tgactacgga aggttatatt
    12601 caaaaaaaac aaggaaaagg ttcgatcgta ctggatcgga atcgcttcga ctttcctatt
    12661 tctggattga ctagctataa agagctacag gagacccaac gtattccaag tgaaacgatc
    12721 atccatacgt tagaagaaac agaagtgaca aaagcattga acgagatcac tggctgggag
    12781 atcggtgcgc ctgtttggca tttgatacgc gaaagaaaaa tcaatggcga agtagttata
    12841 ttagacaccg attatctatt aaaggaaatc gttcctattt taactccagc acaagcacaa
    12901 ggatcgatct atgaatattt tgaaaatgag ctttctttga cgattgctta cgcgcaaaag
    12961 gagatcacag tagaagaagt aacggaaatc atgaaaacag cgatggatct tcacgatgac
    13021 acttatgcag tagtagtcag aagcctcgtt catctggaag atacccgctg tttcgagtat
    13081 tctgagtcgg ttcaccgatt agataaattc cgtttcgttg agtttgcccg acgccgaaaa
    13141 gtgtaaatag ttcaaaagaa agcaaattgt tggcatctgt ccactttggc gctatgatag
    13201 gatcacaaag ggggcatttt taatggataa accaataaga ccagaaaaag aagaactgaa
    13261 agaaaagtta tcacccatgg cttatgcagt cacacaagaa aatgcaacag aacgaccatt
    13321 tagcgggaaa tatgatgatt tttacgaaaa ggggatctat gtagatatcg tcagcggtga
    13381 accgttattc agctctgcag aaaaatatga tgcaggttgt ggctggccat cattcagcaa
    13441 accaataacg aaaagacaag tgcgcgaaaa agccgatttt tctcatggca tgcatcgaat
    13501 agaagttcga agcaaagaag cagattctca tctcggacat gtgtttaccg atggaccagt
    13561 agatcaaggt ggtctacgtt actgcatcaa ttctgcctca ctgaaattca ttccatatga
    13621 aaagatggat gaattaggtt atggggaatt caaatcactt gtagaataat ggaaatacac
    13681 aaagagcggt acaatacggg gatttactcg atcgtccgct ctttttttat tcaagcgatt
    13741 gctatttgaa agaaacgctc cctagactcc ttttttcaca aggtgaaatg tgctacaata
    13801 aagggaacgt atgtgtgcaa aagaaagagg actagaatat gtatgaatat ctgacaggtg
    13861 tggttacctt tatcaacccc tattacatag tagtagaaac caatggtatc ggctaccaga
    13921 tcgcactagg aaatccttac cgctacagta gcaaactaaa tcaaaaaata aaaatttatg
    13981 tccatcaggt aatacgtgaa gatgcgcatc ttctgtatgg atttgattcg ctagaagaaa
    14041 agcaactgtt tttgcgatta gtaagtgttt caggaatcgg tccgaaaagt gcccttgcaa
    14101 tcatggcaag cgaagaccac tccggtttga ttcaagcagt agaatccagt gatgtcactt
    14161 atttgacgaa atttccagga gtagggaaaa agacagcaca acaaatgatc ttggatctaa
    14221 aaggaaaatt cggtgagctg agtatcgata caccttttag tctatttgat gaagcaaaca
    14281 caaaagacgc tacagctcta tctgaagcaa tggaagctct ctcggctctt ggttacagtg
    14341 atcgagaaat caaacgtgta gaaaaacaat taaaagaagt agataatcag acaacggatg
    14401 agtatctacg ccaagcactc aagctaatga tgaaaaaata aggaggagac caaatgacag
    14461 atgaatactt gttatcacca gaatctggcg aaaatgaact gagtttggaa aaatctcttc
    14521 gaccacagct gctttctcaa tatatcggac agagaaaagt caaacaagaa cttcagattt
    14581 acatcgaagc tgccagaaat cgggaagaac ctttagatca tgttctgttg tacgggcctc
    14641 ctggattagg aaaaacgacg atggccatgg tcattgctaa tgagatgacc gtaaatattc
    14701 gtacgacgag tggccctgct atcgaacgtc cgggtgactt agtagcaatc ttgaatgaac
    14761 tggaagcagg ggatgtcctg tttatcgatg agatccatcg attacctaga gtggtagaag
    14821 agatgcttta ttcagctatg gaagatttct atgtagatat catggtagga caagggccaa
    14881 ccgcacatcc ggttcatttc cctcttcctc cctttacttt gattggcgcg acgacacggg
    14941 caggtatgct ttctgcacct ttacgagatc gatttggcat catttctcat atggaatatt
    15001 atgacgaaca ggatctgcag gagatcgttt tacgttcttc ggatattttt caaacagaga
    15061 tcattgaaga aggggcattg gaaattgctc gccgatcacg aggaacgcct agaatcgcta
    15121 atcgcttgct caaacgggtt cgtgattttg cgcaagtcca aggagacggg aaaatcgatc
    15181 gtcctatagc ggatcaggca cttactttgc tacaggtcga tcaggctgga ttggattatg
    15241 ttgaccagaa attgttgcga acaatgatcg agttatatgg cggcggacca gtagggttaa
    15301 gtacgctttc agtaaacata ggagaagagc gtgagacggt agaagatatg tatgaaccgt
    15361 atctgatcca aaaaggattt ttaaagcgta cacctagagg gcgaattgct actgcttatg
    15421 cctatgaaca ttttggttac gactatcata tatgattttt gcttttagat tggaggaaaa
    15481 tgaatgagtg atacaacacg tacgaaacaa ttgatcaaaa acgtattgat tgatttgtgc
    15541 caaacgcaat cttttcgaaa aatcagcgtc caaaaaatcg caaacgaagc aaaaatcaat
    15601 cgacaaacat tttattatca ttttacagat aaatacgatt tgcttcgttg ggtctattgt
    15661 gaggatgcat tgcattattt agatccacaa tgtctttcac ttgataattg ggaagagcaa
    15721 gcattgaaaa tgctgaaagc aattgctgag aagaaagaat tttatgcaaa tactgtgctt
    15781 tctgacccag aaattttaca aaaagagttt tctcaactga tccaaaaatc atttatcaag
    15841 atttttgaac aaatggatga agaaagacaa ctttctgcaa gagacaaact attttatgct
    15901 cgtttttttt cttacggttg cagtggtgta ttagtcaatt ggatcacgaa tgaatatgaa
    15961 gaaacaccga tggaaatcgc tatgcagctt ttccgtttag ccaaggatac ggaattatat
    16021 tcttatcgtc tttatgcaaa agaagagttg gaagaacagt aatagaaaaa ttttttgttg
    16081 gaaaagcaat aatcgctaaa aggagatggg gaaatgacaa aaattttatt tgtttgtttg
    16141 ggaaatattt gccgttcacc gatggcagaa ggcttgctga gaaaacgaat agcattagaa
    16201 ggaagggaag cagaattttt tgtggattct gctgctacaa gcacgtatga agttggaaag
    16261 acaccgcacc ctggaacgaa aaaaatatta aaccaagaaa aagtagatat gacgaatatg
    16321 gttgctcggc aaatcacacc ccacgatttt gaaacttttg attggatcat tggcatggat
    16381 caagaaaatg tagaagaact gaaacgaaga gcaccacgct cagcgcaggg gaaaatccat
    16441 ttgtttttaa gtagcattcc tggaaaagaa aaagaaaatg ttcccgaccc atactatacc
    16501 aataactttg atgagacata tcacttgatc acagaaggcc tgaatcattg gtggaatatt
    16561 tggattgatg aaaataaaat aacaaacaat ttgtaaagtt aacgctttct ttttaaggcg
    16621 atgttagagc atttccttgt ttttttgtaa aatgttaggt aaaataaaat taattaagta
    16681 acactttctt agctaaagga ggagtgcatt cgatgaacga agagacagta tcgccaatga
    16741 agaagcttac gaagttagtt actcatactt cgtatcaact ttttgattct tttgtaaatg
    16801 acactgaaga acatgctaag aatgtacgat tgcaaggaaa gtttattctt gagctgcaaa
    16861 aagctgctac tgaacatagt ctagttatct tacaagtaag agcaaatcgt ttgagtagca
    16921 agtatgaaac gatctcaggc tgggtggcga caaaaacggt cgaaaaagac cgagtgatga
    16981 tccgcctaca aaacgaagag cagcagatcc ggatggttcc tgtagaacaa attgaaaaaa
    17041 taacaatcct ttcaccagca ggggaacagg aacggttatc aaaataattt ataccagata
    17101 gaaaagcacg tatgagcttg taccatcaat cattactaat caaaaataac cgaatgcttt
    17161 aagctgatat cagtaaaacg gaacgtttta ggatgagtat catagcatga cagcattcgg
    17221 ttgattagtt gaatcgattg gatggtacaa gtttttttga taaaaaaagg tcctgctagc
    17281 cggggagcta gcaggataag gagttaaaaa tgaaaaagtg ttgttagggt tgtttgttgg
    17341 tatgcttata taatagtcat tagttgtgaa tattttgtga cgaaagtttt cgaaagttgt
    17401 taagtttt
//
LOCUS       DACECZ010000048        17326 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.048, whole genome shotgun sequence.
ACCESSION   DACECZ010000048 DACECZ010000000
VERSION     DACECZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.048"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(100..645)
                     /locus_tag="IWO35_001502"
     CDS             complement(100..645)
                     /locus_tag="IWO35_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0316563.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(657..1349)
                     /locus_tag="IWO35_001503"
     CDS             complement(657..1349)
                     /locus_tag="IWO35_001503"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ0316564.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1368..1652)
                     /locus_tag="IWO35_001504"
     CDS             complement(1368..1652)
                     /locus_tag="IWO35_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316565.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1665..2228)
                     /locus_tag="IWO35_001505"
     CDS             complement(1665..2228)
                     /locus_tag="IWO35_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0316566.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2456..3118
                     /locus_tag="IWO35_001506"
     CDS             2456..3118
                     /locus_tag="IWO35_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ0316567.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3242..4438
                     /locus_tag="IWO35_001507"
     CDS             3242..4438
                     /locus_tag="IWO35_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ0316568.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4539..5915)
                     /locus_tag="IWO35_001508"
     CDS             complement(4539..5915)
                     /locus_tag="IWO35_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0316569.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLV
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5952..6818)
                     /locus_tag="IWO35_001509"
     CDS             complement(5952..6818)
                     /locus_tag="IWO35_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ0316570.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6907..7932)
                     /locus_tag="IWO35_001510"
     CDS             complement(6907..7932)
                     /locus_tag="IWO35_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0316571.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7950..8624)
                     /locus_tag="IWO35_001511"
     CDS             complement(7950..8624)
                     /locus_tag="IWO35_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0316572.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8808..9701)
                     /locus_tag="IWO35_001512"
     CDS             complement(8808..9701)
                     /locus_tag="IWO35_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0316573.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9775..11472)
                     /locus_tag="IWO35_001513"
     CDS             complement(9775..11472)
                     /locus_tag="IWO35_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0316574.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11465..12778)
                     /locus_tag="IWO35_001514"
     CDS             complement(11465..12778)
                     /locus_tag="IWO35_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0316575.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPQAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESSEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMDELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12778..16815)
                     /locus_tag="IWO35_001515"
     CDS             complement(12778..16815)
                     /locus_tag="IWO35_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0316576.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFYTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVYETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
BASE COUNT         4850 a         3893 c         3016 g         5567 t
ORIGIN      
        1 taaaataacg tataaaaaag agcataaaac aaagtcgttt gttttatgct cttcttttat
       61 tattttgtgc tttttatttt atcaaatcac tttgtttttt tataaaattt ctgcgcctag
      121 tgtggactca aaatgtctca atgcccattc atgtccaacg ggatcgaaac ttgccacggc
      181 atctttgaaa atatggagtt tgtatcctag attatacgca tcgactgctg tatgcaacac
      241 acagatatct gtacatacgc cggtcaaata aatatctgtg atctggcgtt ctcgcaaacg
      301 gatgtctaga tctgttccac taaaagcgga ataatgccgt ttgtctatcc aataaacatt
      361 ttcctcttgc tcatagtctt ggtataaact ttttaaagag ccgtacaatt cccgtccacc
      421 cgttcctatc acattatgcg gaggaaataa gcggttctcc ggatgaaagg aatcttttgg
      481 atcatgcgca tcaatggcaa aaacgacaaa atccttttga tcaataaact tctttgtatg
      541 agcagtcagc tttttttcaa ttacttgtcc tgctgtccca gtagtcagct ttccatcctc
      601 tgcaacaaaa tcatatgtgt agtcaatcga gatcaatgcg ttcatcttag tcctccttat
      661 acgagatgtt tgacaaattc gatcaccatt tctgctgatc gtttgcctgc gtcttcgata
      721 aattcgtcaa agctttgtgt cgccgagtga tctgctgtgt cgctcattgc acgtacgatc
      781 aaaaatggga tattgaactg acgagctgtt tgtccaacag cggctccttc catttcgcaa
      841 gctaatgctt cagggaaatt cgttaagatt tctttgattt tatctggcga gtcgacaaat
      901 gtatcacctg taacgatcaa tccttctttt gcgttcaaat tcgttgcttt tgctgcttta
      961 accatttcac tgcgcaaata tgtgcttgct tcataatata gaggcattcc tggtaattgt
     1021 cctgggttgt agccaaatcc tgttgcatct gcatcaaaat aagctacttt gtctgatata
     1081 acgatgtcac ctacttgtaa gccttcaccg attccgccag ctgaacctgt attgatcacc
     1141 atgttcacgc cgtattgttg gatcaacaga cttgtggtaa tagatgctaa gacttttcct
     1201 ataccggaac gtacgacgat cacttcgtgg ttacctaatg aacctgaaat aaataaagcg
     1261 cctgcgcgtt cccatgataa tggttcgctt aggttctctc tcaaaatttt tacttcttct
     1321 tccattgcgc cgataatacc gattttcatc tttttaattc cttctttcta tataaatgcg
     1381 attgctaaaa aaaccacaca taacattagg ataacaataa taagagattt catcaaaaaa
     1441 gagttccatt tggtcgtctt ttcccgttcg ctgatccttg ttttcgtgat tggtttattc
     1501 tttttgcttt ctttacgata aaagctggca atcttttttt cttcttgctg ataagctgtt
     1561 tctgcttttc gctgtttttt taatgactcg cttgcttgtg cttgttgtcg cttgcgaagt
     1621 tcgcttcgcg taataagcgg tcctttggcc ataagccgat ccctctactt tcctttggtc
     1681 aatagttccc agtattgaat cgcataaatc gtctttgcat cacaaatcaa ctgatctttc
     1741 atcgcttgtt gcgcctcttc aagagttaga tgatacaact ccagtacttc gtcttcatct
     1801 tgtgctaaag gattctcgac cttctctacc ccttgtgcat gataaatatg aaggacttca
     1861 tttgcaaaac ctggtgaaag atacatcgaa gtaagatggc tcagcgactt cgcacgatag
     1921 ccagtttctt cttctaattc tctagctgct gtcatttcgg gattttgtcc ttctcctgga
     1981 tcgatttttc cagctggaat ctccaaaatc actttttcta aaggttttcg aaattgtttg
     2041 acaagaagca gacgatcttg ttcgtcaaaa gcaatgatcc cgacaccgcc aggatgaaat
     2101 accagttctc tcttcgaagt tcctccatct ggcaaacgaa cttcgtctac agcaacatca
     2161 ataatttttc cgctatagat ttctttgcga ctgattgttt tttcttcaaa ttgctcattg
     2221 ttcagcatat cttctcctgc tttctttgta atcataactg cttttttttc atttttcaat
     2281 ttttctcaaa aactccttat tagtttacca tagataaacc ttagattttc tctaaatata
     2341 gaaaatcctt ctttagtccg atactctttc tagtagtccg tatgctaaaa taagtatgta
     2401 atcgaataac gaaaagaatt caatactcaa cgataaatga catggagggt ttattatgat
     2461 aaaacgaaat tggccgtggc ttctagcatt ggtcttgcta tttctctttg tccgaaacaa
     2521 tagtttttct tttgttctag gacttttatt gataggtgta gttttagttt tgtggggact
     2581 ctttggttcg cgcaggaaaa agggaaaaca agaagaaatt cctgcactga ctgacgaact
     2641 tgaaactcac tatgaaaaaa gcggtatgac agcgaatgaa atttcatttt ttcgacaaac
     2701 aatgaatcaa acaaaaaatg agatcaatca attgcaaatg aatatgcagc agacagccaa
     2761 acttaaagca atcgatctcc gccatgatcc agtcaaagca gcgaaagctt tattcaaaga
     2821 attggtgaaa gaaccaaacc gactgcatga agcaagtcag tttctttata cgcatctgcc
     2881 gaatctagtt gatttgacaa ataagtataa tgagatcaat gaccatgaga tcaaaaacaa
     2941 acagacctat gaaaaactag aagaaagcgc gcagatcatc gaccagctgt cagctttaat
     3001 cgcacaagat tatcaaaaat tcgttgctga ggatctagat gatatcgacg tagagatatc
     3061 tgttgcgaaa cagagcttaa agcgtgataa taaatatgaa ccagaacaaa aggattaact
     3121 cttttgttca taaaagaaga ctcggaacat tcagatgatg tctggctgtt tcgagtactt
     3181 tttctaaagt agagacattc atgtaagaat cgagaacaga ttcaacaagg aggaattttt
     3241 tatggatcat aaaccgactg aaacaaaaga tgttacacca gttaacgata cacttgaaga
     3301 tttattgaac aacccgtttt caactccaac agatacgctg accacttcac aacaagcgga
     3361 gattgatcag ctccaagagc aacagacagc tgctcgttta attgacaaac taccggcgga
     3421 acgccaagaa caagctaaac agctagcagc aaaaatcgat gctaatgatg cacagtctgt
     3481 catcagctac ggatcggctg cccaagcaaa attaagtgaa ttctcacaat cgatgttgaa
     3541 tcatgtgcaa gcacaagata ttggtcctgt aggagattcc ttaactgaac tgatgtatcg
     3601 tctccaagaa gccaatcctg acgaactgcg tgccggagaa gggaacatct tccagcgtgt
     3661 cttcgggaag gtcaaacaat cgatctatga agtgacagca aaatatcaaa agattggtgc
     3721 acaaattgac aagatttcag tgaaattaga taaagaaaaa gacggtctgc taaaagataa
     3781 tctgatgtta gaacagcttt accagaaaaa caaagactac tttgatgcac tgaatattta
     3841 tatcgctgct ggagaattga aaatggaaga acttcaaaca acgatcattc cagaagcgat
     3901 gaaaagagca gaagaaactg gcgatcagat ggatgttcag attgccaatg attacacgca
     3961 attcctagat cgtctggata aacgtacgca tgatcttcgc ttagctagac agatcacgat
     4021 ccagcaagca ccacaaatcc gtttgatcca aaacacaaat caggccttgg ctgaaaagat
     4081 ccaagcatct atcgctacag ccattccgct gtggaaaaac caagtcgtga tcgcattgac
     4141 cttgcttcgt caaaaagatg cagtaactgc tcaacgccaa gtctctgaaa caacaaacga
     4201 cttattgaag aaaaattcag aaatgctgaa aatctcagcc atcgaaacag cgaaagaaaa
     4261 tgaacgtgga atcgtggata tcgagaccct tcaaaccact caaaatgatt tgatcgaaac
     4321 aatccaagag acattgcgta tccaaaaaga aggaaaagaa aaacgtcgtc atgcagaagt
     4381 agagcttgga cacatggaag aagatctgaa acaaaaactg ttggatttaa cccaatagat
     4441 cacctttcgc ttgttactaa aacaaacgcc caacaacagt ttattacaac tgttgatggg
     4501 cgtttttctt atctttcttc taacttctcc attggagtct atttctcatt catcttgctg
     4561 tgtccatagc catagccaaa ataaatgatc agtccaataa ccattacgat accgaacacg
     4621 atccatgtca cagtttgcag acgaagcatc aatacaatac aagtcaagat ggagacgatt
     4681 ggcaatagcg gaacgaatgg cactttgaat tctccagctt ttggtttgcc aaacattttt
     4741 ctcaacttca gtaagcctag actaacaagc atcaaatagg cgagcgccat gatattcgtt
     4801 agttccgcta atacattcaa tgggaaaaat ccggcaaaga atacagctgc cacaccagcg
     4861 gcataagtcg cttttttcgg tgtacgatgc ttttgatcga tttctgtcat aaacttagga
     4921 agtaagccat ccttgctgac agcaaacagt aaacgagcaa gtgaatacat catcgagatc
     4981 gttaccgtca gtagcgttaa aattgcccct acagcgatca ccgcgcccac tgttccatgt
     5041 cctacatacc tcattgcaaa agccactggg tcattgactc ctagttcagt atacggaacg
     5101 attcctgtca agatcagcgt cacgatgatg taaaggacag tagcaatcag tatcgaacca
     5161 atgattcctc ttggcacatc tttttgcggg tttttgactt cctcggctgc cattgcaacg
     5221 gcatcaaatc ccaagaaagc aaaaaacaca agagctgcac caactagcac accggaaaaa
     5281 ccaaaaggca tgaatggctg ccagttatca ggtttaacga aaaatattcc tacaatgata
     5341 aacaacgcga tgataccaaa tttgacatat accatggcat tattcaaacg taatgctttc
     5401 ttggcttcaa gcgacacgat gtaagtgacg aatatcacta cgagcaccgc aatcaagtca
     5461 atgtatgtac cattttccgg attgtacccg ccagttaatg cttgtggaag ttcggcccct
     5521 aaacttttca aaaaaccttg catatagcca gaccatccgg aagcaacaga ggaaaccgct
     5581 agtaaaaatt ccccgatcaa caaccaccct gtcagccagg cagcaaattc accaaataca
     5641 acatacagat aggcatatgg cccaccaatc actggcactc gcgaagcaaa ttctgcgaaa
     5701 cttagtccag acaaaatgac aacgattgca gctaagacaa atgaaagtga cagtgctgga
     5761 ccagcatttt gattggccgc aacccctgtc accacaaata ttcccgtacc gatgatcgca
     5821 ccaattccaa gcatgatcaa atcagacgtt tttagttctt ttttcatctc actcgtatga
     5881 ttgagattga tctctttctt acgaaacaaa tccattgaag ttcctcccca ttttaggttg
     5941 gaaaacagcg attaaactgt tttcatatac cctaaaagga taccacctaa aatgactaaa
     6001 aggcaaccaa caataacata aatgagctct tttttcgttt ttctttcgcc aaggatgaaa
     6061 atcccaccta aagtagagat aatgatcccc atttgtgaaa gtgagaagcc aacagctaaa
     6121 ccaagtgatt tgactgttag aagcatgcag atgtttccta agccccataa taatccacag
     6181 atcatgtttt tccacacaaa ctgatcgact ttaactttac ggaaagcaaa gaagcttgcg
     6241 cctaagatca ttccgatact ttgaggtaag ataattgaca tcgcatccaa attcgcccag
     6301 ttgacgatga tggtatatac accataacca atcgtagaga agatcaatgc tctaaaaccc
     6361 ttaccaaaat caagcatttt attatctgta tctatttttc cttcgtcatc ttgtcgggca
     6421 gtcaaatacg caccgcttac aagaagagct aaagcaataa taccgtatac ataatctttc
     6481 gtttgtgtcc attcatggaa gaaaacggct cccgcaatcg tattcaaaat taattgaaaa
     6541 ccagtagaca atggtagccc tacagaaaca cccatgtact tcattccatg gaactgtcca
     6601 ttttgtccaa cactccaagc taagccggat aaaaatccga tcaagaaaat ccaaggggtg
     6661 attacaggac taacaacgaa aaagacaact aaagaaaaca gaaatgcacc gatcgtcatt
     6721 cctagtgttt gttgattggc atcgccacca attttaccac ttactaaacc aatactaccc
     6781 catgccaaca tagggactaa tgctattaaa atttccataa ccaatctcct tttatttcac
     6841 atgataagaa acttatcagc gaaaacgtta acaacacaag cacttttttt cgtttctaac
     6901 gattttctaa cgtttttcat tataaaaatt taagattggg aaaaataaag tcattgttgt
     6961 cccttttcct tcagttgatt cagccgtcaa atggatacct aatttttcgg ccagattttt
     7021 tgctaaataa agtcctaatc ctgttgaatg tgttttgcta agtcgaccat ttctcccagt
     7081 aaatccctta tcaaagatcc gtttcaagtc ttccgctggg ataccgatcc ctgaatcttt
     7141 aactgaaagg ctgactcctt gcgcttcctt ttctatgctc acaatgatgt ttccgtgatc
     7201 cggcgtatat ttgatggcat tactaagcaa ttggttgaag ataaaagcga tccatttagc
     7261 atcggtcaaa acagtatgat cctcaccaac gatctcgtag cgaatatttt tttggataaa
     7321 ataatttgcc tgactgcgta tcacaggctg aatgattcct tttagtccat attcttggat
     7381 gaggtagtcc cgcgagaaac tgtcgagtct tgcataatac aaaacttgtt ctacgtattc
     7441 atcgattttt gataattcat tttctagtaa tgtatacttt tgatcgtcaa tatcgaactc
     7501 gatcgaacga actagcaatt tagaagcgga aagtggcact ttgatttcat ggacccatga
     7561 gtcaatataa tctttttggt cttgttgatt actgattgct gattgcatta cttcttggtg
     7621 ttcacggatc agctgattga tatattcttc taccagtttt tcttcttctg tcctcccacc
     7681 actcaaataa ttttgcaaag gggaagtttt ttgtatgtcg gctaattttc cccaccatct
     7741 tctcttgctc aagtaaccac ccagcagata aaaaatcaga aagacgccct caatcaaagc
     7801 taaataagca attgtgctca aatcaacttt gatgtttggc gccagccaca tcacaaagat
     7861 cgtcaatgca ataaaaaaca gccatcccaa gatcaatgtc cactgatctt tcaaatattt
     7921 aaaaaaattc atcttaaccg ctcatccttt cagggaacga tatatccctg tcctactttt
     7981 gtttcgatat atccttggat acctgcctgt tcgatttttc ggcgtaaccg attgatgttg
     8041 actgtcaatg tattgtcatc tacaaatcgt tcgtcatccc acagtgctcg cagcaatttt
     8101 tcccgactta agatctttcc attgtgcttc atcaaaataa ataatagacg atattcattt
     8161 ttgctcaaat caatgatctc tccgttgatt tccatacttc cgttgtcaac attcaacgta
     8221 atgccattgt ggctcatcat ttcactagat aattctgtgt agttgtaaga acggcgaagt
     8281 aatgcattga tttttgcaat cagtacatcg atttggaaat gttttgtcac aaaatcatct
     8341 gcccccatgt tcatagccat gatcatatcc atattcgtat tcctgctgga gataaaaatg
     8401 atcggtactt tcgatacttc acgtattttt tgacaccaat aatagccatc caaaacggga
     8461 agattaatat ctagtaatac aagctgtggt tcttctcttt caaagtctga gaacacttga
     8521 ttgaaatcgg tcgttccaaa ggtttcaaat tgccatttct caagttcctc catgatcaat
     8581 tgtcgaatga tctcttcgtc ttcgactacc atgatttttg ccataaaaaa tccctccatc
     8641 tttcttcttg ctactacaat atcaaagaag cccttgtgaa acaagttggc ttacctggaa
     8701 aatgtcattt tagcaaacgt taaaaaaaac tggaaatatg cgatttaatc gcgtttgtct
     8761 cttccaagca gcttaaattt attatgttat tcttaataat tggtctatta tctgttattt
     8821 ttctttcgtt gtttttgtct tttaaatttt cgataacacc aataaccgaa aagcgttagc
     8881 actagcaata tacctagaag gagtataagt aatcgataaa gatggtattt ttttgtctgt
     8941 tctatctttg aagagacttc ttctgtaaag ggaactcgtc ttcctgtcac tagtaatcgg
     9001 tgagtgttca cggtgtaagg tgtacaagtg ataagtgtga ctagatcctg cttttctcta
     9061 attgctaatg tatttgtttt tgttggaagg acaacaattt tctcaacgac ttcataagcg
     9121 agcatccgac catacacttc aagatagaat cggtcatctt gctccatttc ttcaagatcc
     9181 gtaaagagtt ttttctcagc aataccgcta tgggcggtaa tcacggaatg tgtactctct
     9241 cctccagttg gaaaagaggt gccttgcagg actgtcgctc ctctatctaa caaaagatca
     9301 ttcgtttcat caaataaagg caagctaaca ttgattttcg gaatatagat ggctccgatc
     9361 atatgttgtt cgtaatattc ttttcctgga ttttggacat ttctaaccgc ttgctcaaaa
     9421 ggatctttga cttgtcccat tcctgggatt actgatgtat gagcaagcgt ctgatttttc
     9481 ttttcatgca ctgctaattg ttgctcatgt ttttcttctt tatcgcgttc aagctgcttt
     9541 tgataattct cgatccttct ttgatccaaa taattgctga gagcatctgc taaaaatgga
     9601 tatgtaaaaa ctgcagctcc ggcaagaaac atcactgcca taaataattg taataaaaga
     9661 ctgatttggg tttgaggtct attacctatt ttctttttca ttttcttcac cttcttattt
     9721 aaaaaaactg cagcaacctt tataaaaccg attgctgcag cttatttttg ttgcttatac
     9781 ttcagacttc attttagatt tacggtacca gctgtacgct ccaatcatca aacttattcc
     9841 gattagtaag aatgccaaga gcccgttccc accagtagca ggaagtgacc cgcgtttcat
     9901 attcggtact ctttctggcc ctggattttc tactcctacg atctgaattt cttcattata
     9961 gcttccataa gagatgataa attcagtaaa ggctgcctca cctgttggaa gtacataacc
    10021 ttctggcgcc atggtctctt tcatttgata ggttccataa tctaaccctt gaacctcaaa
    10081 gtatccattt ccatctgcga caaattctgt tgcttgtccg cttgttgccc actcaattct
    10141 atctgctcct gattcataaa cagctaaagg cgactcccct ttataaaata ttgcgtattc
    10201 agaacggtct tcattccaca attcgaatct tgcatcttta agcaattcac tgcttgatgc
    10261 atcatatttt tcaaactttc ttccacctgt tgtgactttc tctggcggct ctactccttc
    10321 ttcataatca aattcatcat ttcgtccgat ttcaataacc gctgtgttgt tatggaaatc
    10381 atccgcttct agttctgtgg aaaccttcaa attatacgtg atcgttaaat ctcctccacg
    10441 tgcatctgct aatctaccta aagtatctac tttggctact ccattttctt ctgtcaaaat
    10501 cgtcacgaca aacccgttgc ttactacttc tactatataa tcttgtcctt ttgttaaaat
    10561 cgtctcgccg gctcgcactt ctatttcctg atcataataa gttagaccaa ctgtaggcgt
    10621 atctgtgatt ttgtaatttt tgataacagc gcttccgttt tcaaggacag aatcgatgaa
    10681 ataaggaata ggtgctgtga tcgtgtaaga agccgttttt cctctttcta gatcaatata
    10741 agaaatcgtt tcttgttctc cagcttcatt aataatcgtt acttgtctaa ggtcgctttc
    10801 ctctacattc atctgtttat ctgcttctct gatttcgttt ttcggataca aatgcacatc
    10861 tgtattgtat gtgtctttcc atgtagaacc atcccacacg ccatccacca tatcaggcat
    10921 catcactggc atcataacaa ccattggaga cgcgattctg gaaatatgag ctggcgaata
    10981 agtttctaag aaaagataga ctttatctct agaagaaggt gcttcttccg tcacttggac
    11041 ggtagagaat accgcttctc cagcttcatt cgttgtttgt ttgtcaatac ttgtcagtcg
    11101 atattttaaa aaccaacttt ctgtgtgccg tttttgaatc cattcaatcg cttcggcaga
    11161 attaagaccg tcttctcgtt ccggtgttaa aggattatct cgaatcaaat cgtaatatac
    11221 ttccgttacg tcaaacaaat caaaatcgac tcctggaata ttttcttcac caaaatccgt
    11281 agaaaccaat ccgctattcg gtcgttcttc cggaacagat gagaaacctt ttttgtgtaa
    11341 tgtaatcgtc acttcacttg gtttttgtgc gacttcttcg gctttgacta gttttccgtt
    11401 cccgaaaatc cctattcccg cagtaacgag taacagtaca ctcattcccg tgacaatcat
    11461 ttttttattc atttttttct ccccgttctt tttttattaa taaaacagca cctaacaata
    11521 aaagaattcc tagtaaagaa atcaacgttc ttgcttcatt cgtttgcggg aatacggacc
    11581 attttcctga ttgacctgga ttgcttggac cactcggctc tttcggtttg tccgttgttt
    11641 cttttacggt ctcgttataa acacgtggtg ttgcggattc ataggcagag actggaactt
    11701 tcccggattc taattcatcc atctcctgtc cgttaactac tacatattgt ggttggcctt
    11761 ttgcatccac ccaaaattca gggatgatga cctcgattct cttacttgct tcatccatct
    11821 cgtaaccttc cacacctttt aattcttcaa aataataggt gccagaaggc aagaaacgtt
    11881 gtcctgtcgt aacaaggccc tctttatccg aaatgaattt gcttacattt ttgtcattca
    11941 aaggatcatt gtctgctggt ttgatccatt gatttttcaa gtcattagca ggagccatat
    12001 ctaaataata tttcccccct tctatcatct gatatagagc aaaaacagca ccttctaatg
    12061 gttttccttt ttcagttgtt cctctttgac gtccatactt gaagaagtat ggatctctta
    12121 gataaccgac atttttagga tagatatgaa tctcttgcag ttcctcgctt gattctgtcg
    12181 ggtgataaat tggcaaaata gcagcaattg ggatagctgt ctgatcgata tcaacatcca
    12241 atccaacttc tggatcaatc gattcttcga aaatcaaata aactgcatcc cgatcttggt
    12301 ttttcttagg tgctttgata cgtgcaatcc cgtctcctaa tccgccaacc gtatctttcc
    12361 cagttgttac cgaagtccct atttggacaa gcctttcttc tgtgattagc tgacggatat
    12421 tgtttctgtt cgtttcagcg atttgctgga cgaattcttt aggagtcatg ttttcagcag
    12481 cttttttttc atgataataa tcggtcatgt cgtaaatggc aaatgtcgct ccatttagtg
    12541 gcaccgtctg tgaaatgata tctgctgttt cccctgcttc ctcagagata gccaagccat
    12601 cattttcatg gtattcaaat tgatcgttat aatcgatatc tcgaatcatc cgtttatgca
    12661 acacgaattc cacgacatct tcccctgatt cttctgccga catcgcctgt ggaagaagga
    12721 ctgctgcaag cggtagtaaa acaagtaatt gaagcatcaa acacaacagc tttttcatta
    12781 ccttgctccc tcctttttag attgtctgac aaacaaacca taggctgctc ctgcaagcgc
    12841 catgaagaat gcagcaagca ggatatagaa taatcgtccg gatcctcctg tagcaggcaa
    12901 cggattttca ggatcgttcc cgacactaag ctgtaatctg attcgatttg tctccaaaag
    12961 ttcatattct aactcttgct gtagatcagg cgtctctgct tcttcgtttg gataatacga
    13021 aatctcaact tttagtgtac ccgcttcttt tcctggaact acccgatcat tttctatgtc
    13081 aggttcttct cgataactaa tttctaaaat aaagtaacct ggaagttttc tatagctgtc
    13141 aggtgacttc acttccttga tccgatagac cccttccaac aagtctgaag cattgaatag
    13201 ggaagaatcg tttggatctg gtgccaagcc tccctcgatg atttcatact ctcctgcact
    13261 attttgtttc tcaattgaaa attctgcgcc ttcaagacgt cggctattca tactgtcaat
    13321 tttcaaaagt tccaaatcga ttggcgctag ttcatcttct aaggttactt gtagtacacc
    13381 atcgatgatt tcatagcctt ccggcaaggg agcagtttcg tcttcgatga ccgtatcttc
    13441 aatgataaac ttaccttctt cattcagtgc aaactggaac acttgatcag atagccgata
    13501 cccatttggt gcgacagatt ccgagacctc atattcacca atagacagat gcagaggttg
    13561 gtcgtaattt aaatcgacat acttttctac tcctacggca cgagaataga agtatccgat
    13621 cccaggcaaa cgtttcgatt cttctggttc taacggatta ccagtgtatg ctcgatacag
    13681 cgggaaggaa gaatccccca ctttcctgat atcgaaacct gcaccatcta atgtatggcc
    13741 ttcatggtct aattttctaa ctcggatctc ccgataatca aacgtattgt aagcagtgaa
    13801 tgctacccat aaacgatcct cttcatctgt tgtatacgtg agatattgcc aattttcttc
    13861 acctgtttgc ttgagttgga tagtccctat agcattatcg taaagtcgca agatccatgt
    13921 gtcattttca tcaggttctt tgaaaccttc tgggttctgg ctctcaatga ttttgtattc
    13981 tgcatagttg gtagtttctt cactatcctt ataaacaaat gaatctggct ctccgattgg
    14041 atgaataact ttctcaccat ccacgattac atatttcccg tcttcatcta ctgaatagaa
    14101 cgtgagtctc cctttctctt cctctacttc gttcacacca gaaagccctt tcgctactgg
    14161 atatccgatt tccagtccgt taccatctgt ttcataaagt tcaaactctg cgccattgat
    14221 tggcatatta ctgccttcaa ttagtttatt gatattcatg tggattggct tgaattcatt
    14281 tcgcatttcc agtttgacat acaactcatc atactctgtg ccttctatgt taggctcttg
    14341 tacaacagaa agcttgtgat attctccttc ataataggaa ttcggatctt taccttccgg
    14401 tccgccgact ctcgaaccag atgtatattc atctacaagc tcgtagtgac ctaacatctc
    14461 atcttcgttg gctgcatatt ttttgatttg gaattgatac gtctcatata ctaactgata
    14521 acgtggtggt ccagcgatct ctctgatcca aaatatgtcg ctgactgctc cagtttcatc
    14581 tcctaaaatc ggcatcttcg tttcaaagat cgctattccg tccgtggtag ttttacttgt
    14641 ttcgattggt tcggcttccc cactctctgg attttggtca tataatgcaa attcgactcc
    14701 agccaacggc tcgccatttt gacccttctt gctgacttgg aaattacggc ctttatgttc
    14761 gtaacgaatg gaagaagatg ggaaatacat ccgtccagta gtgatatcat tctcataatc
    14821 ctccaggttc tgaatatagg ccatattgtt cactgtgtaa aaatttccgt cttgagctcc
    14881 cgcatcaagt tttacttggt acttgatcct gaccatttcc ccttcaccta aagaaatcgg
    14941 agaaaattta taagcttggt tttcccgtcc gacatacgtt gtcggagtcg ccactacttc
    15001 tggtggggct tgtccatgac gttcttcatc ccatatttct ggctcttggt acttcatgcc
    15061 gttttctcct ggatctagat ggtaaaactg aatatctgcc gatctgccag ctccaaatgg
    15121 tgagacatta aaaccagttt catcataaag tgtggcttgc tggatcgttc tcttgaaggt
    15181 agaagagata tcctccaagg ctgtaacaat gtcattcttg ttgttcgcat tgtaatatcg
    15241 cggtgttacg ccatcttctt ctaaatcagc aatatttttc aatacatttc gtccaatagc
    15301 atctagtcgt tgtctcccgc taacgtttcc atcgattcct aaaccaatgg agaagatact
    15361 tggcgggttc tcttgatact tgtttttcag ccaatgatga taagcaactg tgttgatcgc
    15421 agatgacttt tcttgcgaag taggactgcc attcgtattg ttattagaag aaccatcacc
    15481 aacccagtga gtcgctttat tatctccttc ttttacctct ccataacgat agtgtacatt
    15541 tacttcatct tctggacggt taaagtcaga tgcataggta actggatgag taaaccctgc
    15601 attagaagta ttcccagaaa actcctcaaa atttctgaac aaaacgtcat tatcttcagc
    15661 aatcttatct tccatcacag tccagttgct ccaagctcca ttagtgctcg tacgataact
    15721 tgactgaatc ggtgcataac tgaaggtcgg tgtcccatct cccacaacga ttaggatttt
    15781 ttctggattt ctattttctc cgccattgtc tttatacaat gtttcatagc catttttaag
    15841 ccctagtgta agcggagttc ctccgattgg tgtacgattt aatgctgtat tctcacttcc
    15901 cgtcaaacga ttgatatcat ttgtcaatgg aaagatgtcc gaactgattt gctcatggtt
    15961 attgatagct gtactattat ggtaaaaatt caccataccg attcgaatat ccagactcgg
    16021 gtcagagagc agatctttac tcatttcgtt tactgcctca atcaacgcag catttttagt
    16081 ttgactattg gtcccagcgg ttaattcact catactcgct gacttatcta gaacaaaaac
    16141 gatatcgatt ggactgattt cctttttctc gcccccgatg acatctagat agacattgta
    16201 aagggttgtt gcatcttcag gatgtagatc agatgctgtg ctgcttggca gaacaggttc
    16261 tacccatttt ttgatgtacg ccccgttata atccaagtaa ctgttttcaa aattccgttc
    16321 atttccccaa agctgaacgg tttgagctgg aacgtttcta tcttcttcgc ctgcagttga
    16381 tccattatta gtagcatagt cgtaatttcg ccagttcgct cccccgtttg tataatcact
    16441 atcatcgatc aagttctctc cgtcatatgg cgagcctttg tctccttgat agcgttcatt
    16501 gctttgtgtc caatttccta acacttttgt atagtcattc gttgtaaatt tcgggtagcg
    16561 atgctgttcg ttttcaactt ctccgctagg atagatatat tcataggggt cttgcgtagc
    16621 tgttggccca agacgctcca aaataccgtt gaataactgg cgttgaatgc gctggagtgc
    16681 ttgaccgctg ggtacgattg gttcgtccag cttttccatc tcattttctt cgctacgttg
    16741 aatttcagcg gaatcttctg tatcagtttc attttcttgt aattcttggt tatctattct
    16801 ttcatcaacc accacttgaa gaggtagttc cccttcgttc acatcgggga tcttttctgt
    16861 ttcaaaaatc aatgtgccgg tttctgcttc ttttgaataa acttttccga cataccattc
    16921 ttgaccatcg atcgtctcga cttttaggtc ttctccatct tcatcatagg aggatagacc
    16981 tgttccacga acgtttttta cggtaccaat cccggcggct gccgcatcga ttgctaaacg
    17041 tattctacct tcattggcac tttccttttt ttcaaaagcc agatgccatt caagggtttc
    17101 ttctttttca atatacttcc cttcaatttt tgcatagtcc tcatctaaga atacctcatc
    17161 ctcctttgtt tctgttgact cagcagctac atccagggtc gtcatatgat agactgactg
    17221 gaaatatggt ccaaatagca aactgacagt tactaactgc aaaagcttca attttaaccc
    17281 tttcacatcc ttatactcct ttcaaaaata tttgtcttct ttcaca
//
LOCUS       DACECZ010000049        17083 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.049, whole genome shotgun sequence.
ACCESSION   DACECZ010000049 DACECZ010000000
VERSION     DACECZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.049"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..325
                     /gene="sgrA"
                     /locus_tag="IWO35_001516"
     CDS             <1..325
                     /gene="sgrA"
                     /locus_tag="IWO35_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ0316577.1"
                     /translation="SSESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSV
                     LKQPDFVSRGIDHQEKAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSL
                     FHRVK"
     gene            623..979
                     /locus_tag="IWO35_001517"
     CDS             623..979
                     /locus_tag="IWO35_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316578.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            982..2139
                     /locus_tag="IWO35_001518"
     CDS             982..2139
                     /locus_tag="IWO35_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316579.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(2165..2605)
                     /locus_tag="IWO35_001519"
     CDS             complement(2165..2605)
                     /locus_tag="IWO35_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0316580.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            2765..3271
                     /locus_tag="IWO35_001520"
     CDS             2765..3271
                     /locus_tag="IWO35_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0316581.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            3439..5352
                     /locus_tag="IWO35_001521"
     CDS             3439..5352
                     /locus_tag="IWO35_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0316582.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            5357..6358
                     /locus_tag="IWO35_001522"
     CDS             5357..6358
                     /locus_tag="IWO35_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0316583.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(6560..6766)
                     /locus_tag="IWO35_001523"
     CDS             complement(6560..6766)
                     /locus_tag="IWO35_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ0316584.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(7412..8260)
                     /locus_tag="IWO35_001524"
     CDS             complement(7412..8260)
                     /locus_tag="IWO35_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0316585.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            8424..9326
                     /gene="gnd"
                     /locus_tag="IWO35_001525"
     CDS             8424..9326
                     /gene="gnd"
                     /locus_tag="IWO35_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ0316586.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            9328..10329
                     /locus_tag="IWO35_001526"
     CDS             9328..10329
                     /locus_tag="IWO35_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0316587.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            10326..10610
                     /locus_tag="IWO35_001527"
     CDS             10326..10610
                     /locus_tag="IWO35_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0316588.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            10643..11440
                     /locus_tag="IWO35_001528"
     CDS             10643..11440
                     /locus_tag="IWO35_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0316589.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            11454..12287
                     /locus_tag="IWO35_001529"
     CDS             11454..12287
                     /locus_tag="IWO35_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0316590.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            12305..13162
                     /locus_tag="IWO35_001530"
     CDS             12305..13162
                     /locus_tag="IWO35_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0316591.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            13184..13693
                     /locus_tag="IWO35_001531"
     CDS             13184..13693
                     /locus_tag="IWO35_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0316592.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            13730..14131
                     /locus_tag="IWO35_001532"
     CDS             13730..14131
                     /locus_tag="IWO35_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316593.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            14243..14596
                     /locus_tag="IWO35_001533"
     CDS             14243..14596
                     /locus_tag="IWO35_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316594.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            14630..15073
                     /locus_tag="IWO35_001534"
     CDS             14630..15073
                     /locus_tag="IWO35_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0316595.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            15090..16175
                     /gene="uxuA"
                     /locus_tag="IWO35_001535"
     CDS             15090..16175
                     /gene="uxuA"
                     /locus_tag="IWO35_001535"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0316596.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            16168..16881
                     /locus_tag="IWO35_001536"
     CDS             16168..16881
                     /locus_tag="IWO35_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0316597.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         5966 a         2644 c         3598 g         4875 t
ORIGIN      
        1 gagtagtgag agtagcacag gaagcacgag cagcgaaagc agcacaggaa aagtaaataa
       61 tgaacttgat ttggtaccta tcataccaac taaggaacaa aagagaaatg cagggagcaa
      121 cctaaaaagt gtgctaaaac agcctgattt tgtttctcgt ggaattgatc atcaagaaaa
      181 agcgaaagaa acagataata gaaaaagcga tttaccagaa actggtagta gaaccttgaa
      241 caagctaatt acatggatgg gtgtgttact cattttgatt gtaggagcaa gctattttcg
      301 ttctctcttc catcgagtga aataaagaat aaaagcaaca gatctaaaat tgaataaaac
      361 gatagatttt tgagtgaata tttcattcga gagtgctgta ttgcctggcg aagttgtagc
      421 tttgccagat ctatttttgt ttactaacat atttgagaaa caggaaataa caggaacaca
      481 aatggaaaat aagagcaaga ataattgaca atgcacactg cgcatggtag tatctctgaa
      541 aaatcagtaa cgaatctatt tgtacgttat tcatgtagct gtcggaataa gtctaaataa
      601 tagataaaaa ggagtatgta ctttgtttga agaatttatt gatatcaatg aaaggcaagt
      661 atatcaattt ttgaactatt gttatgaaag agacgagaaa ttatatgtgg tgaaggatat
      721 cgctttagat ctaaactaca ccctggctaa gatgaattcg gtgattcaac aagcagaatc
      781 gttttgcgaa aggtatccag aatataaatt atcttttctt tcagaaaaca agatgataaa
      841 agtcgagttt tcttctcagt ttttactgag taaagtttat tctatacttt tagagggaac
      901 gattggttat attttactgg attcacttta caaaggtact tatcaatcct tagaaaatct
      961 tagtcaaaaa attatataag tttgcgtacg attcaaagaa aattaagaga acttaatact
     1021 attttgaaaa actatcattt atcttgcagc ttgaaaaaag gtaatccgat agaaggagaa
     1081 gaataccgta tccgatactt ttttcacttg atgtattggc agtcattcga cgaaaatcat
     1141 tctgatatat tatcaagtat aaaaaataaa cgtcaactat tagaaaaatt tgcgaaacat
     1201 gcgccatata cgcgtagtat agatagaaag aaatggatca gcctactaag aatttcgttg
     1261 caacgaatga aacaaggcca ctcgattacg tatgtcccag aagagatgaa caaatttgtg
     1321 catccattga taacaaaaga agagtttacg cattacatac tagagccttt ttttgaagca
     1381 aacttctggt cgatgaatca catcactcca acagagcaag cttaccttta ttttatgttt
     1441 ggtgtaatga acacctattt ggaagaagat ttgttggata ctccagataa agtacaagaa
     1501 agttggggct ggattgaatc gtctattgta gaaaattttg aaaaaaactt caagatttgt
     1561 tttacaactt cagaaaaaaa ttatctatat gtcaatttgg caatgatcca tttgtattca
     1621 agggtttttg ggacaaaaaa gaaggtggat gcttttggaa aatcagcaga agacagagat
     1681 tttcagcata tatttccagt gatgtatcca atcatgcaac agttttttag tggggtagct
     1741 cgcagaattc cagagcttaa tacttattat gaaaagaaca gacggttgat ttttcaatac
     1801 tgtatgttag tcagggatat ttttataaaa cacgaacaac ctgtcatatt ctatattcaa
     1861 tcaaagtatg gtaaaacaca ggaattatgg gcaaagaaaa ggatattatc tatgactgaa
     1921 cgcccaataa aatatagtgc atccgctgat cagttacctg atattgtcat tagcgattat
     1981 cctatcgaca aaaaggcata tgatcaaacg aatacacaga tattttactg gaatggacaa
     2041 cctacaaaaa atgattgggc gcgtctgaaa gctgaaatca taaaaatcag gaatgagaaa
     2101 aatagatcca tattggaaaa attggatttc gttaattaag aaaagcagat tgtttatgcc
     2161 aaaattaaga cattgcaaaa agattatttt ctttaaaatc gacaagatga ccagaaagca
     2221 agagttcgtt gagctcttgc tttcttttag ttttagaaag gaacatcctg attttatctt
     2281 cgctaaatcc agtcaaaagt tttgcagatt ggtcgtagat aatcggtttt ttcaataaag
     2341 aaggggatgt acgaatctca atatatagtt cactcaaact gagttcatct attctgtgaa
     2401 caagattttt ataactaata gaatgagtag agagcatttc ggtcacgccg ttgtttgtca
     2461 attttagtaa gtggataaat tcatttttgg ttagtggttg acgaattaag ttctgttcga
     2521 taaacggtat atcatggacg ataagccaac gttttgcgcg tcttgaagac gagcagccag
     2581 aacctgtata caatttaatc atcatacatt cctccaatag ctaagtattt atgaaataat
     2641 aacatgaaga aatagtcgaa aagtgccatt tagaggtgta aaataagaaa atgttgtcac
     2701 gtataatgga actagctaga taagaaatac tattaaatca agaaaaaaga aagaaggcac
     2761 aagtatgtca aacacagccg acgcaatagt agtgatcgat ttacaaaaag gggtatgttt
     2821 tgattcttcg ccgatacacg aattagaaac agtgacagca ttagtgaata aaagaatcaa
     2881 aacatacgaa acgattggga aaccagttat tttcgttcaa cacgaagatg ctcaattagt
     2941 gccacaaaca aaaccatggg agctattgtc ggaattatct attcctgagc acagttattt
     3001 tgtgagaaaa acccatgcga attcatttta taagactaat ttgagtgttt tattaactga
     3061 attggactgt cattcgatcg aattatgtgg ggcacaagca gagtattgta tagatacgac
     3121 tgtgaagttt gctcatggat taggatacca gcttatgatg tgtagaggag caacgacaac
     3181 ttatgataat ccttttatga aagcaaagga aacaatcgct ttttacgaga aaatttggga
     3241 taaacgattt ctgacatttt tggagcaata agttgtttct tcgtttaaaa aaagcagaat
     3301 ggatactggt ttatccattc tgctttatta cgtaaaatga gaaaataatc ctgttctatt
     3361 tttatgggat aaaccattta ttttttgtat aaaaaatgtt tgcgtttaca tataattctc
     3421 ctagaaacat aaaaaggagt gaatagcttg agaacaaaac aagtattaaa gggagcgttg
     3481 gtcatcttat ttgctgtaag tacaagcgtc ccgcaatgga cagtatatgg agagagcatt
     3541 tcttccgaat cggaatctcg ggaggtagaa accacagaaa ctactgatgc tggaaatgaa
     3601 acgaaatcga gtacttcaca aacagaaaca aatactgaaa caacggttga gacatctgtt
     3661 actagcacag atcaagaaac gaccgctacc acagataacc aacaaacaga agaagcaaca
     3721 gctacaaaaa tgtcaaacga cgaatcctct tctgctaaag taagtgacaa agagaatggg
     3781 acagaaaatg atgatatcgg taaaggtgaa aatttcaatg gtaaagaata tttgaattac
     3841 ttcggtaaac agatgacaga aatacttaat ggaccaacag aagacatcaa gtcaaaatta
     3901 atagagttat tagtcactga gcttgctgtt aatgattatg gtgtagataa agagattatc
     3961 aaacatctga ttgctactta tctattgtac gatatcgctt ttgcaaaaaa cagtgtgttg
     4021 atgtcgatgc ttggaggtac agatgatggg acaacgccta cagaaaacac aggcttctat
     4081 ccaaatacaa aacaacttca tcaaactgta ctaaacaaac aagatggcaa aatgatggat
     4141 gtatatgcat attatgtcga tcaaggttca gataaaacag tcatgatcca tggcggattt
     4201 cgagggaatt ggaataacgg gatcgttaca gaagaataca atgattttta taaggccggg
     4261 tataatttat tatttgttga ttccagagcg actggtaata gcggaggcga ttatgtgacc
     4321 tatggacaat atgagtcaga tgacgtcctt tactggataa atcaagaagt tcgagagcgg
     4381 ccatctcaaa agattttact ttatggtggt tcgatgggag cagctacgat gatgtctgta
     4441 ttagcgaaag atattcctgt caatgtcaaa ggaattattg aaaattgcgg atttgcatca
     4501 attgatgaac aactacgttt tacgtacagc cagacagtag cccccgcact accggatgcc
     4561 ataaaaaatc agttagacat cattggggat caagagcatg aagatttatt tatgggcctt
     4621 ttgaaacaat actattttga tcaggagatg catttggata caaaagctgc tctacctacg
     4681 attggaatgt cagattcatt gcctaaattg attatccatg gaacagcaga tgatgtagta
     4741 cctgtgtcta acgcgcaaaa attatatgag ttatcaggag ggtataaaga tcttttatta
     4801 gtagaaggag cagggcatgg aaaggcacag gaagttgacc atgctgccta cacgaaacat
     4861 gtaacggact ttttgaaagt agtgttccat gatcagataa acgtaaagta tgtggatgaa
     4921 aacaataaaa gtctactcaa agaccaggac gaaatccagc tttacggagc ttatggagaa
     4981 aattatatga cagagcaaaa gacattcgaa ggatacgaat tagcaaatgt tgaaggaccg
     5041 actgaaggga tttttaatga gacgatacca actattattt tctattacaa aaaaatccca
     5101 gtagctccac caaaaaagca agatccgaca gagaatgcag acaataaggg aaagaatgaa
     5161 cctcgagagg atcaatcaag tgatgatacg caaccgattc agatgacagt gaaagtacaa
     5221 aaaaagacag atgcaaaaaa aagaatgttg ccgaaaactg gagaaaagaa acattcgctt
     5281 ctcatttatt caggtggatt tttatctgtt agcacgatta tcacatggat ttggcgaaga
     5341 aagagagggt aaatgaatga aatggttcaa gcgaatagtg attgcagtac cagttatcat
     5401 cgtagttatt ttagtcactg tatttttgat taatcaaatc acaccaaagc cgattagctt
     5461 gcttgttaga aaacaatttg acacaagtga aaaagaacaa gtatatgccc gaccagatga
     5521 tttccagcaa aaaatcgaac aaatcgatat aaaaaaagaa atatcctatc cttcaaagta
     5581 caaaaataat aatatggata tctacacacc gaaagtggaa aagaaaaaat taccgattct
     5641 tttctggatg catggaggcg catatgtagg aggggataaa aatgattgtc gtgattattt
     5701 ggaatatcta tgttcagaca cgcagcaaat catcgtaaat atagattatg aacgatcacc
     5761 ggaagcaaaa catcctacac cagttatcca attgaacgaa gccattcaag cagctaaaaa
     5821 agagataaaa gatcaagcag attggtcgaa tatttcgatt ggtggaaatt cggcaggtgc
     5881 acaaattagt ggagaatatt tgttagcact tcaaaatgaa gaaattaaaa aagctgatca
     5941 tcttgatccg actttggaga acaagcagat tacgaagttt atttctttgt ctggtctgtt
     6001 ggatccttct gcacttactc aagtaagtga taaaatctct agctttcttt atgaaaaatg
     6061 tggatgggct tactttgata ataaggactt tgaacaatca gagaaggtca aaagtttagc
     6121 cttggtcaga catgcggatc gttggacgca aaacacattc ttaacggatg gaaatacaaa
     6181 tacgtttacg aaacaaatgg aagaagtaac ttctgtattt gagaaagcgg gggtgaaagt
     6241 cacaaaagta gattatgcgg agaaaaatgc tgttttgaac catgaatatc agtttgattt
     6301 cagcaataaa caagcgcaag aaacatatca aaagttagta atgtttttca aggagtaaag
     6361 tcttatgatg caaaagaggt tgtaatctgt cacttagctc caagtcacaa ggagcaattt
     6421 caataaacaa gcttttcatg gtttatgaat attgtaactt gtgactgagg agttagagat
     6481 ggcaaggggc accttttatt cgaaatttgg gaatatcaca cgattattgt cacgatctct
     6541 tttacgtaaa ttttataagt cagatttgtt gttttgatcg tttaaaaaaa gcaacaaatc
     6601 cgaagcctgc ggaggcaaga agggacaaaa agccgagtgg taagcaaaag aatgctggtt
     6661 ccactaatat tccgttgtta ttgatataag gctgggaaca aagatgaata gcaaataaaa
     6721 tcaatgcact agcgaaaaaa agcaaagaaa tatggatagc tttcataagc agcacctcct
     6781 tttttataat tgtatgataa atagattaag aatctataaa tgtgacaagt ttgttaggat
     6841 gtgaataaaa tgatcatcta gcgaacttga aacaaagatg gctaatgata agcaattaat
     6901 gattcagcaa tcagataggt acaaagttta ccatcatacg tctagaccaa taatagaaaa
     6961 tatgggaata taatagaaaa gatttaggat acccccaaaa gaatttcccc aaaaattcga
     7021 tgtatcctaa acttttttat tttacataac aatcatatca actgtgaaat aaaattacaa
     7081 atgataacct aaaatgaggc caaaagccag tgttatcaag gaaatactta attcaccaaa
     7141 atttttttta attttttaga aaataagaaa caaaaataat aaaaaaaaga aaagataaat
     7201 tttttactac tgtatactaa ctttgttatc gcatctaaca agtaactcga ataataccaa
     7261 tacaaccttc tccaaagatg cgatgacata cgttctaacc caccgactat tgatgttctg
     7321 taccaccctt gcagaaccaa aaaagccact accaaatatt tggtagtggc tttttttgtt
     7381 ttatggattt ttcgcttttt ctgttatggt gctattcttt gatttcagaa atagtatgtc
     7441 gaactttagc gatagaacga cgtgattctt tttcgttgta aaacatcaaa atcacatata
     7501 gagagtccaa aatgcttagt tgagaaatac gggaagctaa agcttcagat cggaattctg
     7561 tttcttcaga aatagaaata aaaacgacat cgccaagttt agcaagggaa gaattagctt
     7621 ggctagtaat aacgattgtt tttgctccag tttttcttac ggtttcagca atatggattg
     7681 tttcttttga ttgccctgtg tgagaaatca aaacagcaca gtctttttcc gtcaataaag
     7741 aagcttccat caattgaata tggtaatccg tgctgtgaga agttgcgata ggggaacgca
     7801 aaaatttatg ataagcgtca gttgctacaa tctctgaacc gccaacaccg aaaaaataga
     7861 cacgtttaga ttcattgata atagtagccg ctttttttag atccttttgt tgtaacaact
     7921 tttttgtgtc atttaaagtt gaaatactag actcgaacac cttttgcgcc atagtgagct
     7981 cagtatctgt tttctgaata ttttcgtgga tagaaatagc agatagatca ttttcttgga
     8041 tcaacatggc cattttaaaa tctttaaatc cattataacc aagttttttt gtaaattgaa
     8101 aaaaagtaga gtcagcaatg cccagttcac ttgctaaatt gctaatagga ctatgactta
     8161 ctttgcttgg attttctaaa atataatcgg cgatagcctg ttcttttggg ctgaagtcgt
     8221 tgtacattgt tttgatttgt agtcttatgg agacgctcat gagaattcct cattttcata
     8281 ataagattat cattttttcc gttctcatta atgttaaaga tacaaggaga gtaggaaaaa
     8341 atcaaacaaa aaatattttt gtaaaatcta tttacaaaaa aatttttttg tataatgagg
     8401 gtataagaaa tggaggcgct atcatggaaa ttggtgtaat tggtttggga aaaatgggat
     8461 tgaatttagc tttaaactta aaagagcacc atcacaaagt acaaggactc gacatttcaa
     8521 gtacagctgt tagtcaggca agagaaaatg acattcctgc ttatcaagag atctctgaat
     8581 ttctagctac ttttgagcag aaaaaaatca tctggttaat gcttcctgct ggagagataa
     8641 ccgaaaacat gttaagaaaa ctttttccaa aactcggacc aggagacatt attattgaag
     8701 gcggaaactc ttattataaa gattcaatcc gtcgagcaga agagtttcag aaaaatgaaa
     8761 tcggttactt ggattgtggg acttctggag gaatcgaagg agcacgacat caagcctgct
     8821 taatgattgg aggagataaa gaaacttttc tatccgtgga acaattattc aaagatgtag
     8881 caattgaaag aggatatctg tatgctggtg catccggaag cggacacttc ttgaaaatga
     8941 tccataatgg aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc caacttgtac
     9001 aaaaaagtga gtatgctttt gacttggaac aagtcgcacg tgtttggaat catggttcgg
     9061 tgattcgcgg ctggctgatg gaaatcatgg aagatcagtt aaacgaacat cccaatttaa
     9121 gtaattatcg gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt gaaacagcgc
     9181 tagaaatggc tgttccagtt ccagtaattg caactagttt gttcatgagg aatctttcac
     9241 aggaagaaga tagtttttca gcaaaagtag tttcagcact aagaaatggg tttggcggac
     9301 atgaaatagt tgaaaagggt gagtaaaatg ccagattatc atatagcaat cgttaattcc
     9361 agcagtttcg gaagagtctt tccacaccac ttggaaagat taaagtcagt tggagacgtt
     9421 catttcttta ctgttgatca agagattcca ggaaaagaac tagcggagtt acttaaagga
     9481 ttcaatatca ttattgcaag tgttacgcca ttttttcgac gagatttttt tgaaaacaaa
     9541 gatgaactgc tattgatttc ccgacatggt attggttaca acaatattga tttaaaagca
     9601 gcaaaagaac accagacaat cgtatctatc atacctgctt tagttgaacg aaatgctgta
     9661 gcagaaaata atataactaa tttgttgtcc atgcttcgac gcacgacaca agcacaagca
     9721 agagtcaagg acgatcgatg ggaagatcgg gcggagttcg tcggtaggac gctgtttaac
     9781 aaaacagttg gtgtgattgg tgcaggaaat accggaagcg gagtaatcga aattttgcgt
     9841 aacggttttc gctgtgatgt tctagcttat gatccttaca aatcagattt atacatcgaa
     9901 agctttggtg caaaaaaagt agaacttcaa gaattactgg aacaatcaga tatcatttgt
     9961 ttatgtgcga atttgacaga agagaactat cacatgattt ctgataaaga agtcgatcag
    10021 atgaaagaaa atgtttatct atctaatagt gcaagaggcg ctctagttga tgaagaagcg
    10081 atgattaatg gtctgaaaag cggaaagatc gcaggatatg ccacagatgt tttagaagaa
    10141 gaaccgggac gtcaagatca tccgtattta gcgtttgaac aagtagtgat tacgccacat
    10201 atatcggcct acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt cagagatgtc
    10261 gaacagatca gtcagcacct tcttcctgaa cgttctgtcc agacaaagag caactatata
    10321 aaaggatgat gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct caattttatt
    10381 tatatgaaga aggtggtatt tcaaaagttt ctgatctctt gaaatcatac ggcgcgaaaa
    10441 gagtactggt cacccacgga acggtatcat gggaaaaggc gctacctaaa ttagtatttt
    10501 tgaatgacga aacgattcaa tttttttatc atcgttattc tggagaatgt agttatgctg
    10561 aagccagacg aatagctaca atcatcaaaa aaatgaaatc gatttcgtag ttggcgttgg
    10621 cggaggaaaa ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa tcgatttcgg
    10681 agtcatccct actttagcta gtaattgtgc tccatggaca cctcttgctg tcatgtataa
    10741 ggagaatgga caagcagaag ggaaaacaga acacttttac agacaagctg ctttttttat
    10801 aacagatccc caattagtga ttgattcccc tgttccatat ttcattgccg ggttagcaga
    10861 tacactagca aaatggtatg aatctgagct catcttagaa caagattttt tgcaagatga
    10921 atcatttttg aagctagcac aagatactgc gaagatctgc aaagaagaga ttttgaatca
    10981 ttctgctaaa gcaatccaag atatggcaca aagaaaactg acatcggagt tcaaacagct
    11041 ttcagagatt atctttgctg ttgctggttt agttggagga ttaggagata aatacgcaag
    11101 aaatgtggca gcacatgcta tgcatgacgc tatcagtaaa tatattccgg aaagccatcg
    11161 ttttctacat ggagaaaaag tagcgtatgg gattttctat caactggcac tagaaggtcg
    11221 ctgggaaaaa atagacgagt tatatccttt gtatcgggaa ttagaactgc cggtttctct
    11281 agaacaaatg ggattatttc cgaaagataa acagataata gataaaatgg ctgaatttat
    11341 tgattcgaaa gagaaagtgc atttgattcc tatagaggta acacagaaat cactgataca
    11401 gtcaatgatg gaactggaag gttatatcag aaagatctga aggaggagga cacatggaaa
    11461 cattaagtgt agtacaaagt ttgttgattg ctttatgggt agccgcggtt atgtcccgct
    11521 ggttaggtgg aggagccacg ttgaccttac gtttttctcc tttgatgact ggattagtcg
    11581 tcgggttgat catgggggaa gttgcacaag cgatgattgt tacagccgct ttgcaaatga
    11641 tttatatggg ggttttttct ccaggaggat caatgccagc tgaaccatct attgcagcag
    11701 ctatcgcagt accagtcgca ttattagggg atctgtcgcc agaagcagcg attgccgttg
    11761 cggtgccagt cggattgctc ggaagttact tgtatcagtt tcgttttttc atcaatactt
    11821 ttttaggtaa atatacagac aaagctgtag aagatttgaa tacaaaagct atggcccgat
    11881 caattattct ttatccaacg gttgcctcat ttttattatt cgttccgctt gttttcattg
    11941 ctttatattt aggggcgcca ttgatcgctg atgtaatcaa agctttagag ggaacagtcg
    12001 ttatccacat tcttgaagta gtaggtggag ggttagcggc aatcggtatc gcgacaactg
    12061 tatacgttat cggtcgaaaa gattatctgg tctttttctt ccttgcttat tttatgagtg
    12121 ttgttttgaa atctttagat attacgatgg ttacttatgc catttttggg attattatcg
    12181 ctttgatctt tgtccagtcg caaaaaggga aacaagtaca gcctgccaca agcaataaca
    12241 attcagcatc ttttgatgac gaagatgact atgatgacgg attttaattt aggaggaatg
    12301 ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca cgaaaaaaga
    12361 tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact cttttgaacg
    12421 ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac tttataaaga
    12481 tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca ctcaattaag
    12541 ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa gagcagaaga
    12601 agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca cgaaagcggg
    12661 attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg tccaatatat
    12721 ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg cactttttcc
    12781 ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa gacaagcttt
    12841 cactatgggc cgtaatgcta caggcctaaa gcaaaacact ggtgttcaga cagcgattga
    12901 aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact atgtaaaagt
    12961 agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg aaattcttga
    13021 tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact tgttctatac
    13081 gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat taattgtgct
    13141 agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg tagtcaattt
    13201 agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat ctcaaaaaag
    13261 tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat ttatgagaga
    13321 tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta caccagagaa
    13381 agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt tgatcgcaaa
    13441 aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag aattgaatat
    13501 tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat ctgtttattt
    13561 aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg taaagaatat
    13621 ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat tgaacaagtt
    13681 tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa tgaagaagaa
    13741 cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc gccgtacaaa
    13801 aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta tgagaacaga
    13861 tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt atgttggaga
    13921 tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc atgcattagg
    13981 aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact ctatattatt
    14041 gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata acaaaaatcc
    14101 tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag catttgggag
    14161 ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa cttttattcg
    14221 aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg atccgagtgt
    14281 agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca gcgtgagatc
    14341 gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag atacaccgct
    14401 acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag ttgataagtt
    14461 gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc agttcttcta
    14521 tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca atcagattat
    14581 tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa tgttggaaat
    14641 catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg aagtaattgt
    14701 cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca tccaaacatt
    14761 cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca tgctagtatt
    14821 tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca atgaactgac
    14881 tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa tgttgttaga
    14941 agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac aaattgtcaa
    15001 acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa aattagatga
    15061 ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg tatggtgaaa
    15121 aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat gggataattg
    15181 gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt gaggagttga
    15241 aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta gcgatccacg
    15301 atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat atacgaacaa
    15361 tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa ccaatttttg
    15421 gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct cttttatacg
    15481 atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata cacagccagt
    15541 caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc gctcagctgg
    15601 aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact tattttttag
    15661 aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat ccagatgatc
    15721 cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac ttgaagaaaa
    15781 ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga tctttagggg
    15841 caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc atccattttg
    15901 tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg gcacatccct
    15961 ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa gaagggttcg
    16021 acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct atgcctggat
    16081 atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat gaggcgataa
    16141 gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat ctttgatatg
    16201 gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa atcagcacaa
    16261 aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca aacagaaatg
    16321 gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat caaatggcga
    16381 gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt atatacgaaa
    16441 aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac aatcgtggct
    16501 tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct atatgccttg
    16561 tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc agagatcttt
    16621 ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt tgaagactcg
    16681 attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat caaagatcat
    16741 ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt aagtttatat
    16801 gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg atttttagaa
    16861 agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta tttattgtcc
    16921 tgcacgactg agggaacaga tgaaaaagaa gtaatttttt attttgaagt atctacctta
    16981 tttgtagtga acaatttaga acaaacacaa aacgcatcta ctgttatcta ccagtagatg
    17041 cgtttaaaga gaagaacgag aatcgtccat atgagggttt cca
//
LOCUS       DACECZ010000050        17003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.050, whole genome shotgun sequence.
ACCESSION   DACECZ010000050 DACECZ010000000
VERSION     DACECZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.050"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            118..600
                     /locus_tag="IWO35_001537"
     CDS             118..600
                     /locus_tag="IWO35_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316598.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            797..1687
                     /gene="pstS"
                     /locus_tag="IWO35_001538"
     CDS             797..1687
                     /gene="pstS"
                     /locus_tag="IWO35_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ0316599.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(1974..3248)
                     /locus_tag="IWO35_001539"
     CDS             complement(1974..3248)
                     /locus_tag="IWO35_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0316600.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            3521..5992
                     /locus_tag="IWO35_001540"
     CDS             3521..5992
                     /locus_tag="IWO35_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0316601.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(6095..7831)
                     /locus_tag="IWO35_001541"
     CDS             complement(6095..7831)
                     /locus_tag="IWO35_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ0316602.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7828..8532)
                     /locus_tag="IWO35_001542"
     CDS             complement(7828..8532)
                     /locus_tag="IWO35_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0316603.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8663..9166
                     /locus_tag="IWO35_001543"
     CDS             8663..9166
                     /locus_tag="IWO35_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ0316604.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            9126..9464
                     /locus_tag="IWO35_001544"
     CDS             9126..9464
                     /locus_tag="IWO35_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ0316605.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9485..10903
                     /gene="ffh"
                     /locus_tag="IWO35_001545"
     CDS             9485..10903
                     /gene="ffh"
                     /locus_tag="IWO35_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ0316606.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            11015..11593
                     /locus_tag="IWO35_001546"
     CDS             11015..11593
                     /locus_tag="IWO35_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ0316607.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11597..12118
                     /locus_tag="IWO35_001547"
     CDS             11597..12118
                     /locus_tag="IWO35_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0316608.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(12197..12751)
                     /locus_tag="IWO35_001548"
     CDS             complement(12197..12751)
                     /locus_tag="IWO35_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0316609.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            13004..13279
                     /gene="rpsP"
                     /locus_tag="IWO35_001549"
     CDS             13004..13279
                     /gene="rpsP"
                     /locus_tag="IWO35_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ0316610.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            13291..13542
                     /locus_tag="IWO35_001550"
     CDS             13291..13542
                     /locus_tag="IWO35_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0316611.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13667..14458
                     /locus_tag="IWO35_001551"
     CDS             13667..14458
                     /locus_tag="IWO35_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316612.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14628..15149
                     /gene="rimM"
                     /locus_tag="IWO35_001552"
     CDS             14628..15149
                     /gene="rimM"
                     /locus_tag="IWO35_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ0316613.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            15139..15900
                     /gene="trmD"
                     /locus_tag="IWO35_001553"
     CDS             15139..15900
                     /gene="trmD"
                     /locus_tag="IWO35_001553"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ0316614.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            16036..16383
                     /gene="rplS"
                     /locus_tag="IWO35_001554"
     CDS             16036..16383
                     /gene="rplS"
                     /locus_tag="IWO35_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ0316615.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            16641..16934
                     /locus_tag="IWO35_001555"
     CDS             16641..16934
                     /locus_tag="IWO35_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ0316616.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         5594 a         3072 c         3502 g         4835 t
ORIGIN      
        1 gattaaatga ataaactctc atgccattat ctctaaaaaa aaagtttttc cccctaattt
       61 ttttagatag aaaattgtat agtatgatga gagaaaaagt agtttggggg aacgttaatg
      121 agaagaaaag cagtaatcga tcggctttat ttattttttt ggttttttcc tatcgttttg
      181 ctacctttta catgggtaaa tgctgttgta caaggtgcgg agatattagg cctgatccaa
      241 ggaagcggat ttgtggtttc taggaatttt ccagctttga ttgttttcta tttagtgata
      301 agtgtaggtc tcttattacc aaaatctctg atcaatcact acaattggca gctgctttac
      361 ttttcttgct tattagtatt tacatctatg ttttctttgc tgcctcgctt gattatcggc
      421 cctaatggtt tgagcgagag tggattgaag gatttagctt attcctccat gtatttcttg
      481 acgatcaaac catggttcta tttagtaatt gcctcattta ttcttgcaac atttttttac
      541 ttctggacag aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa taactattga
      601 tggtggatca tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc tcatatgctt
      661 tttttattat tatgtaaaga atgcgaaaaa cgtttacatg atttttacat aacttaacta
      721 tatttttaca gcaaccatac gagggattta cagtcgactg ataaacttta gttgtaaata
      781 agaaaggagc aataaaatga aaaaagtatt gtttactcta ggtatgatgg gaattctttt
      841 agccggatgc ggctcaggat caggaaatac agatggctct gccacacaaa ataattcaag
      901 tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg acagcattac agccgttagt
      961 cgatgcagca caagaaagct ttgtacaaga aaatccaaat tatcaaatct ctgtacaagg
     1021 aggaggaagc ggaactggtt tgagccaagt cgaagctgga gcagttacta tcggaaactc
     1081 tgatgtattt gcagaagaaa gagacggagt tgatgcttca aaattagtgg atcataaagt
     1141 agcagtggtc gggatggcac caatcgtcaa taaagataca gatgtcaaag atatcaccaa
     1201 acaggaattg attgatatct ttacaggaaa aatcactaac tggaaggaag ttggcgggaa
     1261 agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg cgacattcga
     1321 aaaatgggga ctggatggtg ctacccctgt gcagtcccaa gaacaagatt catcaggaac
     1381 agttcggcag ctggtaagtc aaacaccagg agcaatcagc tatttagctt tctcatatct
     1441 cgatgattcc acacaagcac taagtatcga cggtgtagaa ccaaaagaag agaatgtagc
     1501 agataacagc tggggaatct ggtcatatga acatatgtac acgaacggaa aaccttcccc
     1561 tgaagtccaa aaattcttag actacatgat gacagaagaa atccaagaag gacctgtcaa
     1621 agaattgggc tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg aaggaaatat
     1681 caaataacta gcagataaac atgccacggt agtcgttact attaaggaaa aatcaactat
     1741 gtttccccac atatacattt ttcctttaat tgtgaaaaat agttcctcat attttgccgt
     1801 tttttgagct aataccgagg gtcagctgat tgaacaccgt ttattcgaaa aaaagagcta
     1861 cgacatagaa ctgtcgtaac tttttttggc tcttcgtcaa atcgtgttgg tgaagacaaa
     1921 tttgaataaa acttagtccg atatgcggcg cgttctgtgc gccgcatatt tttttagttt
     1981 tcaaagatca gaccttgttg taaatagatc cgtgcccgaa agttataaaa gttgcgataa
     2041 ccaaacgcaa ttcgtttaat cagcttgatc ttgttattca gcccctcagt gacgccgttt
     2101 gagtgggaca ccttaaaggc atttgaaata cccgattggt agcaatggaa gatcgtgaat
     2161 ttctttttaa acgactgagg taacgtagat gaaattgttt taagggtttc ttgaaagcct
     2221 tccgtacctc gtttaacgaa ataatagcgt aataattgac aaaaatgata cgcagttcgc
     2281 aattcttcgt tgtagcttaa cagctcatca acgatttctg tttgactaat tggtcgttta
     2341 aacagtcgat gataatgccg tttgagtggc tcccatgtcg tggaatcttt gagtaagagt
     2401 ttccaatatc gcttgagccg acggtacttc ttctgatttt cagagtgtga ggttcgaaaa
     2461 cgattcatga tctggacacg taattgattg aagctgcgat tgatgtgttg gacaatatga
     2521 aagcggtcag ttacgatctc cgcatttgga aagactgttt taagcaactg acaatagctg
     2581 gcattcatgt ccatcactag gtacttgacc tttaaacggg cctttctagg aaagcgcagg
     2641 aagtattgag ttagtttgta aagtctgcga tcttcaagga tcgtaaatag ctgattggtt
     2701 gtcccatcaa cacaaataaa actcatggaa ccagaaacag aattcattga tttgaactca
     2761 tccatacaca acaccgtcgg aaggaagttc atacgtggct ggtaatgctt cgttgcttca
     2821 cgcaagatcc gtgtcaccgt cccatccgag acgaagtact tcttagcgat tagtttacgt
     2881 gattgatttt cgtacagttc catgagaatc gcctgcttca gttcctttgt tagttgatga
     2941 tgttcatcaa ctaagtccgt tttcgcagaa aaggtccttc cacaatcatg gcagagaaaa
     3001 cgggtacgtt ttaattctaa aatagttaac ttttctttga ctttcaacat ctgagtgcgt
     3061 gtcgtatagg aaccgttgcg gataatacga accgatcgac agtgttcaca ataggaagaa
     3121 gagtcatcca attttccttt aataaactgc gcttgaacgc ctttatattc tttttcaatc
     3181 agccattctt catcaaaaaa taaatgttta tctgttaatc cgagtaattt tctagtatga
     3241 ttatccattg agagactcct ttggtaggat gacgttttgg tcgactaaat tttaccaaaa
     3301 gtgagtctct ttttgagttg aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt
     3361 tccaccaaca caaaatatta tagaaccctt tttttttgct atatgacagg aatgaaaggt
     3421 gtttgcaagc ttaatgtgag gaacactttc catatctgtt ctatactgga taggagaagt
     3481 ttggaaatat ccagaaaacg aaatcaatcg aggtagccac atgaagaata aatggataaa
     3541 tcgaagtaaa aaaaagaaag taacacaaag atcatcagat aaagtcatcc gtctttttcc
     3601 atcgtataaa gaaggattaa cggcagatca agtcaaggaa cgaatagaaa aaggtgcagc
     3661 aaataattca gtcgatccca cattcaaaac aaatcagcaa atcgtattgg aaaacatatt
     3721 tacgtatttc aatttgatct ttcttatctt agccattttg ctttgtttag tagaatccta
     3781 taagaacttg acttttttac cagttatcat agccaataca ggaatcgcca tttttcagga
     3841 aattcgatcc aaaaagatct tggacgaact aaatgtttta aatacagcaa aagtcaatgt
     3901 catacgagac gggatagaac aaacaataga tatagaagac cttgtaatag atgacattgt
     3961 tattttagag actggatatc aaattccagc agatgcagag gtcgtggaag ggaaactgca
     4021 agttaatgag gctttattga caggcgaagc agatgaaata accaaagaaa taggtgatcc
     4081 attaatgtcc ggcagtttca ttgtttccgg aaaagcttac gcccgattag acaaagttgg
     4141 agcagattcg tatatttccc agctgacagt caaagccaag acaatgggag aaggagaaca
     4201 gtcggagatg attgcttctc ttaatcaatt gatcaaatgg attgggatca tcattattcc
     4261 tatcggtatc ctgctttttt ctcaaagtta cttttttaat ggaaacacga tcaaagaaag
     4321 tgtcgttgca atggaagcag cattgatcgg tatgatacct gaaggactgt acttgctgac
     4381 gacgattgca ttagcattaa gtgcgactaa actagcaaag caaagagttc ttttacacaa
     4441 tatgaaaagc attgagacat tggcaagggt aaatgtcctt tgtgtagata aaacaggaac
     4501 gatcacagaa aataaaatgt ctgttcaaaa aatgatcgtc tctaaaaaac aagaaataat
     4561 cagtcagtca gatcaattgg aagcatgcat tgctgactat gcaaaagcga tggcaggaga
     4621 caatgcaaca atggaagctg ttaaagcata ttttacaaaa accactgatc aatcctatca
     4681 ccatgttatt cctttttctt ctgtccaaaa attcagcagt gtagcgttgg cagacaaggt
     4741 gtatgtttta ggagcgcccg aaatggtttt gcgcgatcaa ttgccggaat atgcggaaga
     4801 gtttgtttct ttcgctgaaa aaggctatcg cgtgttggtc tttggtgtgt acgatgggat
     4861 tcttgaagaa ccagtattga cagaagcagt cacaccatta ggctatattc tgattgctaa
     4921 tcctatcaga aaagaagcaa gagccacatt tgattacttc aaaaaacagc aagtagccat
     4981 caaagtgatt tctggagata atccgctcac cgtatccaat gtagctgtcc aagcagggat
     5041 ttcacatgct gatcagtacg ttgatgcatc taaattatca gaagaagaat atgaagcagc
     5101 tgtcgaaaaa tatacagttt ttggacgcgt caagccagaa caaaagaaaa tcttcgttca
     5161 attgataaag cgtaacaata cagtagctat gacaggtgat ggcgtgaatg atattctagc
     5221 catgaaagaa gcagactgca gtatagcgat ggcttccgga aatgaagccg ccatgcaggc
     5281 ctcacaagtg gtcttacttg attctgactt ttcacgcatg ccagaagttg ttgcagaagg
     5341 acggcgcgtt gtgaacaata ttgagcgatc agccagcttg tttttagtga agaatatttt
     5401 ctcctttttg ctctcactgt tttcagtgat ctttgctctg acctatcctt tagaaccatc
     5461 acaaatcaca ttgattagtt tgtttactat cggacttcca tctttcttgc ttgctctaga
     5521 agaaaacaaa aagcgaatca gagggaaatt catcatgaat gtgatggaaa aagctgtgcc
     5581 aggtggacta acagatatga tcgtagtcgg tgcgttggtg atctgcgggg tgactttgaa
     5641 tctaaataaa acagatgttt ctacagcatc taccatgctt ttgattgcag tagggttcct
     5701 cgttctctat aaaatctgtt caccgctgaa taaattcaga agtcagatca ttcttttctg
     5761 tgctagcggt aacttttttt cggtcatctt tttgcataag ctgttttcaa tcaccggtat
     5821 ctcagctgta tcacttttgc tgttgagcat tctatttttc tcggcagatt cgatttttag
     5881 gcatttgacc acattggtag aatatttgtt tgaacgtaaa aatgaaaaag agcaaaagcc
     5941 gtttagtcgc gtagctaaaa aatttctatc gatatttaaa cgagaaaatt gacgaaaagt
     6001 atctcaaaaa agagtaaaaa agaacaacca ctcgtgtatc tttcgaagca ggatgagtca
     6061 tccaacttta aaacgcacga gcagttgctc ttttttacat tttcggaatg gtaacggtga
     6121 aagtcgaacc tgttccaaga tgactgtcta cagtaatcgt accattaagg agttgcacgt
     6181 agtcttttac aatcgctaac cctaatccgg tcccgcctga atgtcggctt cgcgccttgt
     6241 ctacgcggta aaaacgctca aagatccgtt cctgatcctc aatgtcgata cctataccaa
     6301 agtcttggac agaaaacgat agatcatctg ttgcctgata gcggataata atctcgctgt
     6361 cgctttttga atattggaca gcattttcaa tcagattttt gataattgga taaaggatat
     6421 ctgttttagt agtaaatggt tcgttctttc caatcaatcg aatcgtcaac cgtttctttt
     6481 cgatgattgg ctgataattt tgaacgattt gctggaaata aggttctgta taaattgtct
     6541 ggatctcgta agaattctcg ccatcttttg acagctggat gatttcccga atcagtttat
     6601 ctaaccggat cgcatctttt tgcatgatct caagaaaaga agtcagagtt tgcgggtcat
     6661 cttttgcacc atccagtaaa gtctcggtaa aaccaatcag tgaagtcaca ggagttttta
     6721 attcgtgaga gacgttgcca acgaaatcct tttgtagctt ttctaaacgt cgcactttcg
     6781 ttaagtcata cgccacccct agaatctgtc cggtaccttc tgaattagta aagtagcgca
     6841 aactcaagtc taatgtctgc cgaccttctg taagggtgat ctcctgatgg atcagcggat
     6901 gctcaggagt cacttgatga atcagctgaa tgagttttgg ctcttgaatg acttctgtgt
     6961 accgctgttc aggttgatag cttaccacgt ttaaatgtag ctgcatcttt ggattaagca
     7021 gtagtaaacg agaatcagca tcatcaatca ggaatacgcc aatcatcagt tcgttcagta
     7081 acgtatataa ttgctcttct gtggtagtac acgcccgata catcttactc atttgttcgc
     7141 tcaacgcatt aatcgtctga tacaattctt cccattgatc gggtgtttgc atgattactt
     7201 ccgcctgatt aggattttgc accatttttt ttagtacggg cagcacggtt ttcaatggct
     7261 ggtttttttg atagatcaga taatagatca ttcctgcaag aacaaggaag aaagacagaa
     7321 agaagaaaaa gacccaacgg cgaaaactct cagttctcgg taaaaaaacg cttgtcggtt
     7381 ctgcgatacg taaaatggct tctaactggc cacttttttt gactggtaat gcgacatata
     7441 aaagatcctc attcaaagta gtacttttgc gcaaagcgct acctaggttc ccaccattca
     7501 atacggcttt gatttcggga cgggagtttc gctgttcatt caatgtttca ttgtttgtgt
     7561 caaataagat ctttccagta gcatccatta aagtaatccg ttcgttcgac tgatggacga
     7621 attcttccaa agcagttttg ttttgcgctg cttggaggtt ttcgacatct aattgccgga
     7681 tcagcagttg tgcttttttt gtcaggtagc tttcttgctg ttccacgatc tgctgttgaa
     7741 aataatggga aatcaattgc cagccaccaa aaaacaaact aactaaaaga ataagaaaga
     7801 agagacgttc ataaattttt ttccatttca tcgtttgggt tcctgaaact tatagccaaa
     7861 tcctctaata gtcaccaagt actttgggtg ttttggatct cgctcgattt tttctcgcaa
     7921 atgactaacg tgtacatcta cgattcggct ttgtcctgcg aagtcaaaat tccaaatgcg
     7981 gtctagcaat gtgtctcggt cgatcacacg gtcttttcgt ttcatgaaat aaacaagtaa
     8041 ttcaaattct ttcggtgtta gttcaatcag atggtcgtct acggtcactt gataattggt
     8101 caaatcagca gtgatttgtc caatcgatag atattcaggc tcagcttcat cttttgattg
     8161 cttacgaggt tcaattcgtc tgaatattgc tttcatacgg gctaatactt cgcgaggact
     8221 aaatggtttg gtcaaatagt cgtcagcacc tatttctagg ccaatgatcc gatccacttg
     8281 gtcatcttta gccgtcagca tcaaaatcgg tgtttcattt ttttcttgac gcagccgctg
     8341 agtgatctcc atgccatcca tcgttggaag catcacatct aaaatgatga aatcaaatga
     8401 ttgttctaat gctaattcta atccttcgcc tccatctgtg gcactggtta ccttgtagcc
     8461 ttctttttcc aaattgaacg tcaagagcgt tacaatagaa ggttcatcat caacaactag
     8521 tatttttttc attcaaatct cctttaaata ggtttcattc tgtttatatt ttagcataga
     8581 atcagtacaa agggctattt tacaaaagaa ataaatggta taataggaag gcactttgct
     8641 gaatagaaag gaagagcagt caatgattgg aatcagtgca tgtttaggcg gtctggcctg
     8701 tcgctatgat ggacagaaaa aagaagtacc agaattgatg gaactagttg aaactggaga
     8761 agctgtcatg gtctgtccag aagtgatcgg cggattacca attcctagag atcccgctga
     8821 aatcattggt ggaaatggat tcgatgtgtg gaaagatcaa gcaaaagtat ggacgacaag
     8881 cggcgaagat gtaacggaag cgtacaaaaa tggcgcgatc aaagcctatc aaaaattgca
     8941 ggaaaaacac attgatctag tcatcatgaa ggaaaaaagc ccttcatgcg gaacccattc
     9001 gatttatgac ggaagttttt ccggaataaa aaaaaatggt gccggagtgg ctactgctta
     9061 ttttattcag caaaaaatga ctgtgctctc tgatgaagaa tggctgaaaa ctagaggtga
     9121 gcagaatgga aatcgaaaag accaatcgaa tgaacgcgct ctttgaattt tattcgacct
     9181 tattgacaga aaaacaaatg aactacatgg aattatatta tgcagatgat ttttcattag
     9241 gtgagatcgc ggaagaatac gaagtaagcc gacaagcagt atatgacaat atcaagcgga
     9301 caagcaagat cttggaggat tatgaaaaaa aactccatct tttttctaat tatattgttc
     9361 gagaacaggt attggaaaat atgactgcat atattacaga taagtacccg gaagataaaa
     9421 aactacttga atatgtacag caaattcaag caatcgaaga ataaataaaa ggaatgaaga
     9481 aaatatggca tttgaaagct taactgaacg tctccagcag gcgatgggaa aaataagaaa
     9541 aaaaggaaaa gtatcagaag ctgacgtaaa agaaatgatg cgggaaatcc gccttgcctt
     9601 actagaagcc gatgttaact tgcaagtagt caaagatttt actaagcgcg tacgcgaacg
     9661 tgcggtaggg gcagaagtgc tggaaagttt atcacctgca caacaaatcg taaaaatcgt
     9721 tgatgaagaa ttgacgatca cacttggttc tgaaacagca gagttaaata aatcaccaaa
     9781 aataccaact gttatcatga tggctggtct tcaaggggca ggtaaaacga cctttactgg
     9841 taaattagcc aattatttaa agaaaaatga aaatgctcgt ccgctcctaa tcgcagggga
     9901 cgtatatcga ccagctgcga tcgaccaatt gaaagtatta ggtcaacagc tagacgttcc
     9961 tgtatttgat atgggaactg aagtcagccc ggtagagatc gtccgtcaag ggatggagtt
    10021 agcaaaagaa aagaaaaacg actatgtatt gatcgatacg gcaggacgtc tgcatatcga
    10081 tgaaacattg atggatgagt tgaaacaaat caaagaattg acgcaaccaa atgagatttt
    10141 gcttgttgtc gatgccatga ctggtcaaga tgccgtcaac gtagctgata gcttcaacca
    10201 gcaattaggt attacagggg tcgtcatcac taaacttgat ggggatactc gaggcggggc
    10261 agcactatcg atccgctcag tgacaggcgc accaatcaaa ttcatcggtt ctggtgaaaa
    10321 attgactgac ttagaagtat tccacccaga tcgtatggct agccgtatcc ttggtatggg
    10381 ggatatgctg acattgattg aaaaagcgca gcaagactac gacgagaaaa aagcggaaga
    10441 acttgcacag aaaatgcggg aaaattcatt cgatttcaat gactttattg aacagcttga
    10501 tcaagtgatg ggtatgggcc cactggaaga tctaatcaaa atgatcccag gaatgaatca
    10561 agtccctgga atcgaaaacg tcaaggtaga tccaaaagat gttgaacgca aaaaagcgat
    10621 ggtttattct atgacgccag cagaaagagc aaatcctgat ctgttgaacc caagccgtcg
    10681 tcgccgaatt gctgccggtt ctggaaacag tgttgttgaa gtgaatcgaa tgatcaaaca
    10741 attcaaggaa tcaagaaaaa tgatgcagca aatgtccaaa ggggatatga acatcccagg
    10801 tatggaccaa atgtttggtt caggcgtcaa aggaaaacta ggcaaaatgg cgatgaatcg
    10861 aatgatcaag aaaaataaga agaaaaagaa aaaacggaaa taagatgaaa aaaacagctt
    10921 cggtacagac tgaaaatgtc tactgccgga gcttttctat cagcaatttt tattctttac
    10981 taatacaatt actctaaacg aagaggtggc acagatgaga tgtccgtgga gtgaacaaac
    11041 agaaagtatg cggcaatatc atgatactgt ctggggtgtt cctgaatatg atgaccaaaa
    11101 gctgttccga aaattgatgc ttgatattaa tcaagccgga ttgagctggc agacgatttt
    11161 aaataaaagt gaagcattcg atgaagcata tgatcgattc gatatcaaaa aagtcgcaaa
    11221 ttatggagaa acaaaaatca gtgaactaat ggaaaacaaa ggaattattc gtaaccgaag
    11281 aaaaatcgaa gcagccgtcc aaaacgccaa agcagtcttg aaaattcaag aagaaatcgg
    11341 cagtttttca gagtatcttt ggtcgttttc taatggaaaa atcgttgatt cttcttatca
    11401 ttcacaagaa gaagtcccaa caacaagtcc tctttcagat gaattagcaa aagatctaaa
    11461 gaaaagagga tttaaattta tcggaagcac aacaatctat gcttttttag aagctgtcgg
    11521 gatcatcaat gatcaccttg acagttgttt ccgcaaagaa gaggtcgtac gtatcggcag
    11581 aaagggcgaa taaagcatgc gaaaaatcat attttatggt gctataagtt tagatggcta
    11641 tttggcaacg aaaacggatg atcttcaatg gctttttgac actccaacag gtaagcaaac
    11701 tacttatgaa gctttttatg aaacaatcga tacgaccatc atgggcagaa aaacttatca
    11761 ggaagcaaaa aaatatttgg agaccgaagc aatttatcca gagaaaaaga atttcgtttt
    11821 ttccacccac gcacaattac aattgccaga tgctgaggtc gtaaacgaag atcctgttgc
    11881 ttttctaaaa aatctacaaa tgacagaagg aaaagatatc tgggttgtgg gtggcggtcg
    11941 attgatcaaa ccgctattgg aacatcaaat gattgatgaa tggtatatcc agattacccc
    12001 tgtattatta ggggacggga ttcctttatt ccaaaaagga acatatgaag cgcgttttga
    12061 aatgctagac acgacacgat tcggtgaatt tatcgaattg cattatgtac gaaagtagaa
    12121 gtaaataaaa taaaaaaggc aactatatgc agaaatgtcc tgtacatagt tgcctttttt
    12181 attaccgtta tgctttttac tctgttaatt tgttagaaaa atctagatac gcatctaaaa
    12241 tcgtttggaa gaaacttaat gttccttcaa cgatattacc attttcatca attagattcg
    12301 caacgtttcc gatatatgct tccggttgct gcaaggttgg cacattcaaa aagacaagtg
    12361 attggcgtag atggtggttt gcgccaaagc cgccgatccc acctggtgat gcagtaacga
    12421 ctaaacctgg tttatgatcc caaacactgt gtccataagg tcttgagcct acatctaaag
    12481 catttttcaa tactgctggc acagaacggt tgtattcagg agtaacgaaa acgacaccgt
    12541 ctagtcccct cacttcctca cgaaaacgag tccattcagc aggagcctgt tctggagttt
    12601 cgagatcctc attgtaaaat ggcagatcat cgatttttac aaatacactt tcgtaccctt
    12661 caggaagcaa tttcgcaaaa gcttctgcta cttttttgtt gtaagaatct tttcttaaac
    12721 taccaataaa aaatccaatt ttttttgtca tacgattttt cctccttcaa actatctata
    12781 ataatcttac aaaagataag taactggagc aaataaaaac acttactgac aaaagctaca
    12841 tagaagatag attacttatt tttaacaaaa aagaagtcta aaatcttcta ttttctaaaa
    12901 aaaatcgaat ttaatcaagc tgtaaagaaa aaaagcttta cagggtaaaa aaaaactgtt
    12961 acactacatc ttgtgaataa aacaatggag gtggaaataa ataatggcag ttaaaatccg
    13021 tttaaaacgt atgggttcta aaaagagtcc gttttaccgt atcgtcgtag ctgattcacg
    13081 ttctcctcgt gatggacgtt tcatcgaaac tgtaggtact tacaatcctt tgaaagaccc
    13141 tgcagaagta gttttaaaag aagacttagt tcttgactgg ttatcaaaag gtgcacaacc
    13201 ttctgacact gtacgtaaca tcctttcaaa agaaggcgtt atgcaaaaac accacgaagc
    13261 taaattctca aagaaataag gtgaccggat atgaaagatt taagcgactt agtcttaaca
    13321 atcgttcgtc cgctagtcac ttatcctgat caagtcaagt tagacgtcgt agagtcgaaa
    13381 gacttttacg agtacaatct aacagtggct cctgaagata ttggccgtat cattggaaaa
    13441 caagggcgtg ttgctaaagc aatccgcaca atcgtttacg gtgttcggat caatgcaccg
    13501 aaaaaagtgc gtttgaatat catcgatgat aaggaaaagt aaataaacgt gctgtactcg
    13561 aattttcgag tgcagctttt ttgttttttt tattaattga agaaatgatt atttggcaaa
    13621 acaagcaaga aagcgtatcc ttataggagg agggagtgaa cagccaatga gaaagaaaat
    13681 gttatttatt ccatttgctc tattgttaag cgcttgtagt tcggttactc aagaatcaga
    13741 caatacacat ctatcaagca gtatatctga aactgttacc agtccagaaa aaagtagtac
    13801 caaaacggct tccaccactg aacaaacaat ggctacctca aataatgaga aaaagactgc
    13861 tttagaccag ttgaaagaac aacagccaaa tgttcccatg cctctagatg taccagtctc
    13921 ttcaggctat ctcaatatcg cagcgacaca tacaaagcaa ggttattcta ttttatatta
    13981 cagaacagat cgtcccctcg ggttgaatgc tgacgaattg aatcaagaaa cgcctattgc
    14041 aacctatcta tatcaatatg ggtttgcttc tagtcaagaa acaatccaag ttttgcagcc
    14101 atttgagatc gacacaaatg gtcagcaagt ggacttaggt tctcgaatca cagggtatca
    14161 gcaaggtgca gcaggttcaa gttttcttga atggcaagaa ggaaactggc gtattcgaat
    14221 ccgtgggaat aatatagaag gccaagatcc cttactatta gctaaagaaa tcgtagctta
    14281 tttggaagaa aacagtctac cagcacctga acaatttggt aaaattactg ttgatatggg
    14341 tgatacgacg aatcgagccg tagaagtaag ctggcaagaa ccgaaaaatg cctatacgat
    14401 cactcatcaa gatcctatgt ctgcgttgaa gatggcagtt tctatgaaga ggttgtgaaa
    14461 tcagcgcgct gatctgagcc catcatttaa ctccaagtca caggaagtaa tcaaaaatag
    14521 cacgccgcat ttttgcataa ttttggctta ataccttaaa agaaaactag aattgcctct
    14581 tcacacatgc taaaataaaa cagacaaaaa agaaatgggg atataagttg actgaatact
    14641 tgaatgtagg aaaaatcgta aacacacaag gtatcaaagg cgaagtccgt gttatctcaa
    14701 caacggattt cccagaagaa cgttacaaaa aaggaacggt gctaacacta ttccaagacg
    14761 gaaaagcacc tgtggaatta acagtcaaaa gtcatcgcaa acataaaaat tttgacttat
    14821 tgagttttga aggacatcct tctatcaatg atgtggaaaa atatcgggat ggcatattaa
    14881 aagtgtcaaa agacaactct gtagatctag ctgaaaatga attctattac catgagatca
    14941 tcggtctgca tgtattagaa gatggaactg agttaggtaa agtaaaagaa atcttgtcac
    15001 caggtgccaa tgatgtttgg gtcgttcaac gtccgaaaaa agctgatata ttgcttccat
    15061 atatcgcttc tgtcgtcaaa gaagtcgatt tagaagctgg agtggtccgt gtagaaattc
    15121 cagaaggact gattgacgat gaagattgat gtattgactt tatttccaag aatgtttgaa
    15181 ggtcctatgg gggaatccat catcggtaag gctgtagata aaggattatt agatatcaat
    15241 atctctaact tccgcgatta ttcagataac aaacatcaga cagtagatga ttatccatac
    15301 ggcggtggag caggtatgct gctcaaagtc cagcccatct atgataacat ccaagccatt
    15361 gaaaaagctg caccggatgt caaaaaaaga gtaattcttc ttgacccagc tggtaaacga
    15421 ttcgatcaga aaatggcaga agaattttca caagaagaac acttagtatt catttgtggt
    15481 cattatgagg gatatgatga acgtatccgc acattagtga cagatgaagt ttccttagga
    15541 gattatgtcc ttactggggg agaattaggt gcgatggtca tgatcgatgc aacagttcgg
    15601 cttttgccag atgtgttagg gaatcagact tcagctcaaa cggattctta ttcaaccggt
    15661 ttattggaac atccccaata tactcgcccg gcagaatata aagggatgaa agtaccagaa
    15721 gtattgacaa atggaaatca taaattgatt gaagaatggc aattaaaaga atcactaaga
    15781 aggacctatc aacgtcggcc tgatcttttg gaaacacttg aatggacacc tcaaatgacg
    15841 aaatttttag aagaaatcaa gaaagaagaa caagaaaaaa cagcagatga agccaagtga
    15901 ctttttctaa aatcttcttt acagtaggtt gataatatgt tagtatttta tggtgagtgc
    15961 aaaagcactt aactattacg atattccgct gtgagaaaag ctcataagaa tatttggaat
    16021 aaggagaaga aaaccatgaa tccattaatc gaagaattaa caaaagaaca attacgttct
    16081 gacatcccag cgttccgccc tggtgacact gtgcgtgttc acgcaaaagt tgttgaaggt
    16141 actcgtgaac gtatccagtt atttgaaggt gttgttatca aacgccgcgg tgctggaatc
    16201 agcgaaactt atacagtacg taaagtttct aacggagtgg gcgttgaacg tacattccca
    16261 ttgcatacac cacgtgttgc aaaaatcgaa gtagttcgct acggtaaagt acgtcgtgca
    16321 aaattgtact acctacgtgc attacacgga aaagctgctc gtatcaaaga aatccgtcgt
    16381 taatcattgt tatcagactg agaaagaacc cttggtattc aagggttctt ttttcttagc
    16441 atgtaatttg aacaccctga tccagttttt tctttaatta ttggtagggg agaataatgg
    16501 attagaataa atctacctat aagtagtagt aaaaaaaaga gcactatgat aatatctttc
    16561 ttaagagtaa ccgctttaat aaaaaatcca gaatattcta acataaatgg agtattctta
    16621 gaatcaaaag gagaaagaag atggtttata cttatacgga gcaagaaaag atgaaagttt
    16681 tagacacatt catgaatgat ggaaaagtaa aacaagtacc aagttcaaca aaaaagaaat
    16741 acatcctatt gaaagaagtc ttgaaacgct ttgaccatgg agtgacttat acagaaacag
    16801 aggttaattc gatactgctg aacgtatttt ctagtggcga ttatgtcgaa caaagacgtt
    16861 atctaatcac atttggcttt ttcaaaagaa gttcagacgg tcgggcatat cagatgatgg
    16921 gaattgaaaa ttagaagttt taatttatac tcattgaaaa aagttcaagt atgcgatatt
    16981 ggttctacac cgcatacttg aac
//
LOCUS       DACECZ010000051        16820 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.051, whole genome shotgun sequence.
ACCESSION   DACECZ010000051 DACECZ010000000
VERSION     DACECZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.051"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..456)
                     /locus_tag="IWO35_001556"
     CDS             complement(<1..456)
                     /locus_tag="IWO35_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0316617.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
     gene            complement(561..1424)
                     /locus_tag="IWO35_001557"
     CDS             complement(561..1424)
                     /locus_tag="IWO35_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0316618.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1620..3719)
                     /locus_tag="IWO35_001558"
     CDS             complement(1620..3719)
                     /locus_tag="IWO35_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0316619.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="IWO35_001559"
     CDS             complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="IWO35_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ0316620.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5072..6913)
                     /locus_tag="IWO35_001560"
     CDS             complement(5072..6913)
                     /locus_tag="IWO35_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ0316621.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7095..9488)
                     /locus_tag="IWO35_001561"
     CDS             complement(7095..9488)
                     /locus_tag="IWO35_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0316622.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9595..10455)
                     /locus_tag="IWO35_001562"
     CDS             complement(9595..10455)
                     /locus_tag="IWO35_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ0316623.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10514..11035)
                     /locus_tag="IWO35_001563"
     CDS             complement(10514..11035)
                     /locus_tag="IWO35_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ0316624.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11355..12074
                     /locus_tag="IWO35_001564"
     CDS             11355..12074
                     /locus_tag="IWO35_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0316625.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12100..14691
                     /gene="mprF"
                     /locus_tag="IWO35_001565"
     CDS             12100..14691
                     /gene="mprF"
                     /locus_tag="IWO35_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0316626.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="IWO35_001566"
     CDS             complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="IWO35_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0316627.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16434..16790
                     /locus_tag="IWO35_001567"
     CDS             16434..16790
                     /locus_tag="IWO35_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0316628.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4617 a         3490 c         2946 g         5767 t
ORIGIN      
        1 tttttctttt tcatagcatt cctgacagat tccatggaac gttagccgat gatcttttac
       61 taaaaaatga taacgttctt ctactacttg ctccacttca agtaaaagat cctcttctac
      121 ttcctcgatc gtgccgcagt tcaagcatag taaatgatga tggaaatgtt tggcgccttc
      181 ttttctcaaa tcataacggg ctaatccatc attaaaactt actttatcga caattttcaa
      241 ttctgtcaag atttcaaggg ttcgataaac agtagcaagt ccaatttccg gactcttttt
      301 tttcactaaa aagaagattt cttctgcaga taaatgctct ttctcatttt ccaaaagcac
      361 aagcagagta gcttctcttt gcggtgtcag cttaaacccc gcttcatgta gctgctgttt
      421 tgtttttttc atcgccgaaa tagcattcat gaccatttac tcaaccccaa caatttataa
      481 taattacaaa taaaaaaaga tataacacta tgttaattaa aattattata agctagttaa
      541 gaaaaacatg caagggattc tcaattagat tttttgacta gttttgtcac accgttttct
      601 tgtgtaatca atccttgttt caaaagatta cctaatgcgc gcttaaatgc tcctttgctg
      661 ataccaaaag ctttcatgat ttcttctgga tccgatttat ctgtaaagtt gatttgctga
      721 tctgcagaac gttctaaaat ggttaaaatc atttgtgcat catctgatat agcttcatat
      781 gctcttggct tcaatgaaag attcagtaca ccgtctggtc ggacaccaat cactcgacct
      841 tttatctgct cgcctagacg aggttcatta tatcgttcag aaggatggat aaacccaaga
      901 tagaaatctt ccgttaaaac gtacgtacct gttaatttca agcggtatgc tgttcctatc
      961 acatctttgt ttttcatttc ttctgttcca tatttgctta aagatttgaa tattttttca
     1021 tctgctaaac tgccccaaat acggtctttt gcatcaactc taagggaaat catcaagcga
     1081 tccccttttt taggccatag ctctgtcatt gtcggcaatt catcaagaga tacgacaaag
     1141 tctttatctg gaagaccgat atcgacaaac gcacctaaat cttttcgtgt agcagtaacg
     1201 attccaaaag cgtaatgacc gatacggctt ttaggaattt gtgtagtgat ccgattttca
     1261 tgtttttgat tttggtaacc gaatccttca actgcttcac cgattttgtg ttcaccttct
     1321 gatttgctta agccaaaggt ttgtccgttt ttttggacaa agtagttttt ttcattttca
     1381 tcaataataa tacctgtaaa tatagttgct aataaatcgt tcattttttt ctccctctat
     1441 agtgaaaggt tttcttaata catttctttg ctatcataac ataatgttat ctacttgaat
     1501 agctgagcct tggtttggaa aggtgaaaaa aggagtatga caacagttgt gtcatactcc
     1561 cctttttcga ataaacggtg ttaaacggca ggctcacagc ctctttcatt tgcctaaaat
     1621 taattgctcc catttcctcc gaaccatcca gttttacagt tgatggttac gatgtaaaat
     1681 ggcaaagcac tttctccaat gcctttcatc aatgcagatt ttgttgaagt ggcatataga
     1741 ttataatagc cttccccatt ttcgattctg gctttttcta atctgtaacc tccttcaacg
     1801 atatatccac gttggataca tgtagcaatc actttgcttt ggacacctgg tgcccacgtc
     1861 catgctggat tagatgcatc ggcaatatca cttgctctgg cagatttgat tggctgattc
     1921 gctgagtttg aagaggaagg ggtcgaggat gccgcttctt gctgtgctgc ttgtgcgacc
     1981 gcagcttgtt gagcggcttg agctgcagct gctgcttgag cggcttgttt tgcttgttct
     2041 tcagctgctt ttttcgcttc ttctgctttt ttcttttcct cttcttcttt tttctcttta
     2101 tcagtgagca tcttatctac ttgtttcaat tgttcattca actgagcaga tagatctgta
     2161 tttttcactt tttctactgc ttcttttgcc gtcttgtatt ggtcgcgtgt tgctgaatcc
     2221 actacttttc cgtctttaaa gactatagct gttttttctt tagctgtttg caattcagca
     2281 tattgatttt tagcttctgt ttgtgcttgt tgaatcaatt ttccaaaatc tgtgttatcc
     2341 gtttcgattg cttggatctc tttatctgct ttcaacttga tgtcatctgc tagatgatca
     2401 tcgacgatgg caggtgctat gaaccactca ttcacttttt ggatatctcg gatcttagat
     2461 tggatatctt ggtaagttgt ttctaactct gaatatcctt tttcattctt aagactggat
     2521 aaatcatttt ttagttttga taaatcttga tgaatcatat ctgcacgaat aaattgatgg
     2581 tgatcatctg tataaaaggc agcaagtttc tcatgaagtt cttccatttg ttgctgtctt
     2641 tttgcttctg ctgctacttc ggcttctact ttttgatgat ggtcataata agcccagctt
     2701 cctccaccga gtagaagtac tgctgctaaa acgagataag gtactttttt tgattttttc
     2761 ttttgcgctt tatctggatc tgttttttct aagtttgatt cttgatgtgt tttttcagta
     2821 tctggcacga ttgttattga tttcgcttct gcttcaaaca gcttaggctg gttctcttct
     2881 ttttctattt ttgcttcaag atcagtaccc tctttgctta cagattgatt ttcagcatcg
     2941 gattcactat ttatttcatc gggctctgtt gactttttgt ctgtgttttc gagtatttct
     3001 tttttctctg cttccggcag tttattcgtt ttttcctttt ctaattgttt ttcagtaaat
     3061 acttcagttt tttctgtgat atccgttttt acgtctttta tttttactgt ttctttacct
     3121 gtggcgtttt ctctatcaga gtctttttca gtttcttgtt ccttttctgg ctctactttg
     3181 ttttcctgtg caattttttc actgatcatt ttgagcagct ctttcgcgtg ctcttctttt
     3241 gtatcttcac gatgtgcttt gatataagcg gatagtatcg aattttcttc ctcattatcg
     3301 acgatttctt tcagttcagc tactcttctt ttttgttttg attcaagcgt ttcttcttga
     3361 ttctttcctg attcttccga agtctcatga acagttgctt tcttctgttc atttttctct
     3421 atattagtta cttgatcgtg agcaggtggt tcttcgtctt ctggttctgc tttttcgaat
     3481 aattccatta cagattctag aggtaaatct ttcaattccg accattcgat tggatcgttt
     3541 aattctgtat caggaaagac tgcatcttca gttgatttat cctgcatagc tgcatctgta
     3601 tttgtttctt caattgattc ttttatttgc tgatcttctg cagttatttg atgaccgcag
     3661 tctggacaga tagtattctg ctccattttt ttcgatccgc aatttggaca ttttttcatc
     3721 acttagcgat cggctccttt actaggttta cgctaccatt taatctatca tattctatac
     3781 gtcacgccaa tagcttattt ttaggattta cttaaaaata atgaatgaat ttccttccta
     3841 tagaaaaaaa agcttgagac tgcgtagtct caagcttttt ttcttttaaa ataaaccaaa
     3901 cagttttact gatttctctt ctggtcaata gtccacgctt attctaagaa atcttttaat
     3961 tgttttgaac gagaaggatg gcgcaatttt cttaatgctt tagcctcgat ctgacgaata
     4021 cgttcccgag taacaccaaa tactttccct acttcttcca atgttctcgt ccggccatca
     4081 tctaaaccaa aacgtaagcg aaggacattc tcttcccgat cagtcaatgt atctagtaca
     4141 tcttccaatt gttcttttag taactcataa gctgcgtgtt ctgctggact tgttgcttcc
     4201 tggtcttcta tgaaatcacc taagtgagag tcatcttctt caccgattgg cgtttcaagg
     4261 gaaacaggtt cttgcgcgat tttcaagatt tctcggactt tttctgttgg aagatccatt
     4321 tccgcaccga tttcttctgg cgtaggttct cttccaaggt cttgcagcaa ttgacgttgg
     4381 atacgtatca atttattaat tgtttcaacc atatgaactg gaatacggat cgtacgtgct
     4441 tggtcagcga ttgctcgagt gatggcttga cgaatccacc atgtagcata ggttgaaaac
     4501 ttaaatcctt tgcggtagtc aaatttttca actgccttca tcagtcccat gtttccttct
     4561 tggatcaagt caaggaactg catgcctcgt cctacataac gtttagcgat acttactact
     4621 aaacgaaggt ttgcttctgc cagacgctgt ttcgcttctt ggtcgccttc ttcgattttc
     4681 aatgctaacg ctacttcttc ttccgcagtt aaaagggaga cacggccaat ttctttcaga
     4741 tacatacgaa ccgggtcatt gatttttaca ccggttggcg cagaaagatc ttctgttttt
     4801 gcttgttcag cttttttctc agcactcttc aagctgtgaa ttgatggatc accattttca
     4861 tccaccacac tgatacctgc gtcttcaatc ttttggatca atttgtccat ttcatctgca
     4921 ttcaaagtga atggtgtagc aagctgattg gataaatcat catatacaac ttgtcgtttg
     4981 ggtttgtttt ctttgatgaa agctgctact gctgcttcat acgttttttt atctgttcca
     5041 tttgccataa aagaaggccc cccttgagta gctatttggc ttgttttaac tgtttcgtta
     5101 aattgatgat ttcaatcgtc aattcaagct caagctgttt gtttccagtt cgtctggctt
     5161 cttgctgcat ttgttttttt aatgttattt catcagctaa gccagatttg ctgatcacat
     5221 ctagcaagtc ttggatttct cgtggcgaac tttcttctgc caccggtaaa tattcgattt
     5281 ccaccaccaa ccgccttaaa ttttcatctt gcaaaaagtc taaaaactct gcaaggttga
     5341 attcataatg ttgtgagaca tatgcatcga ataaaaaata aacttctgca tatgcatcat
     5401 gaataaactg aaattctcgt tcttttaaaa tattacggac agaaacttct ttaaatgcac
     5461 gatataatag aatctgctct gccttttcta cttgcgtcaa aggtctcttt ttccgtgcag
     5521 tttcttgtct cgtaacctga gtgtgttgac tttcctgagg ttcttgacga ttttgctgat
     5581 ttgtccttcg aagatcgcgc agttgttttt gtatcgtctc tctgccgatc tgaaactctg
     5641 ttgacagttg agatagatac atatcttgtt cgattggtga aacaactcgg ctaagttcaa
     5701 ctagtaattc ttcgaggtat tcgatcttgt ctttttcatt atctaaattt ttcccttgtt
     5761 ttaaataacg tgatttaaaa gtaaagactg tttcgcgccc atgagccact aattcttgga
     5821 aagatgcttc tccatactta cggagatatt catcaggatc aagcttttct ggaacgacta
     5881 cgacagaaag tgctaatcga ctgttttgcc gaagcaattc aatccctcga ttggttgctt
     5941 caaaacctgc gttgtctccg tcatagcaaa agaccaattc tttcgtcagc ttttccattt
     6001 gttggatctg ttgagctgtt agacttgtcc ccatagaagc taatccagtt ttgataccag
     6061 cctgccatgc agcaatcaca tccataaacc cttcgaacaa caaaatttct ccgtttttcc
     6121 ggatatcgct tcttgcttta tcaagattaa ataaaacttg tcttttattg aacagttctg
     6181 tttctggact attcaagtat ttgggctgat ctttttcctg ccctttttca ggtgcaagcc
     6241 agcgtcctga aaaaccgatc gtctggcctt gagaattacg aagtggaaac atcactcttt
     6301 gataaaaacg atcagacata cggccatcat catgcgtgac aaacaatcca gagcgttcta
     6361 acaattcatt tgaaattgat tcattcttga atacttgctg aagaaaagaa cgctcattag
     6421 gtgcaaaacc tatgttgaat tcttcaatta attctaaaga caacccacga ctttgcaaat
     6481 attctaaagc tggctgtcca gcttttgtat gcaatagcat gtgatgatag atttccgctg
     6541 ctttttcatg agcagcaatc agtttccccg tagcagattc aggattttgg acagctatag
     6601 tacgctgacg ccattgatct tctaacggta tctgttcgaa gtctgccact tttaccactg
     6661 cttctggaaa acttagacct tctagttctt gaagaaaagt aaacacgttt ccgcctttac
     6721 cacaaccaaa gcaatgaaaa atctgcttgt cttctgcaac agaaaaagac ggtgtacgtt
     6781 cttcatgaaa aggacacagc cccaagtaat ttttaccaga ctttttcaac tggacatatt
     6841 gtccaatcac ctcaacgata ttcgtttgac tgcgtactgt ttcgataacc tcttgaggaa
     6901 tccgttgagc cattttttca ccccctgctc tattgatatc ttttgggttt aacagaagat
     6961 atttcaggag aataaccggt aaaaaatcgc actaactgaa atcagtgcga ttttaaagga
     7021 cacaattata catgtttgat tgtatcatat ttcattgctt tttcaagtat aacacatttc
     7081 tatctcatta agcatcaatt tgctactctt ttcagtaact gattttgaat atcagccttc
     7141 cagtacaata ctcgaagaac aatatttacg agcatgaata caaagaagta tcctacgctc
     7201 tctcccaacc ctaagtttac ttccaaaatt ccatggaaca gcattgcagc tacatatatc
     7261 cctacgatcg tcgttactgc taggaagatg cgcaatgggt tgaagggata acaagctttt
     7321 acaactgcca tgcaactgat cccaatcagc aagtaataca tcaaagtagt agtttcagca
     7381 tttccccatg cgaatgtttt tccaagtaga tacaccgcaa tgatattcac tactacaagc
     7441 aaggcatttg gtaaagcatt aataagtgct gtaggcaaat aacggtaagt gatttttctt
     7501 ttatcttgtt caaaagataa aaagaaggaa ggataccctt caatcgctaa atcaatcagc
     7561 gtgatttgga tagggataaa cggaaatcca attgctgtaa cggcacatat aatcgataaa
     7621 ataaatgaat aaatcgtttt gataaaaaag atgcctgaga ctttcgtcac attgttgacc
     7681 actcggcgtc cttcaaacag gacttctggc aatgtagtaa aatcagagtc caacaatacc
     7741 agattcgcaa tctgacgtgt tgctccatca ccttctgcca tagcaatgct gcaatctgct
     7801 tctcttaacg ccaaaacatc attcacaccg tcgcctgtca ttgcaaccgt tctgccgctt
     7861 tctttcaatt catttaccag caatttcttt tgctgcggac tcactcgtcc aaaaaccgta
     7921 tagcgatgag cagcttctcg cacttccatt tcttcagtaa tttgagaaag atcgacatac
     7981 gcatcataat tcggtaatcc tgcacgtcta gcgatattgg agacagtaaa aggattgtct
     8041 ccagaaatca ctttcagatc cacaccttcc tcatgaagat aagctagtgt ttcttgagcg
     8101 ttttttcgga ttggatcatc gatttcaaac agagcaaccg gctcaacaaa tgctggtttt
     8161 tcttcgttca attgctgatt tggagcgatc cccaacataa gtacgcgata gccattcgct
     8221 tgggctgttt ttagactgtc cggaagccga tcttcttcca ccaaacgttc aggcgctcct
     8281 acgtacacga ctcccagttc tggaaattcc atcgcgcccc atttgcgatc agaagaaaat
     8341 gcgactttgt ttgtcacatc ataccgatta gaaagctcaa aataatctct catagcccgc
     8401 atagtgatgt tgttatcact gctttctgcc agatacgtcc ccataatttc tgggatggct
     8461 ttctcgtaca gcggatgtag tgcttctacc cgctggatac tcattttccc ttcagtaatg
     8521 gtccctgttt tatccaagca taatgtatcg acatgggcca gcgtttcaat tgaatacatg
     8581 tcttgaacaa ggattcgttt ttttgccagt ttggttaccc ctgtcgttaa tgcaatactt
     8641 attagcaata ccaaaccctt tggaagcatg cctaacaaag ctgctgcaga tgcaataaca
     8701 gaaattttga tagaagcatc gcggatgaac agtgcttcca aaaatagtat gatccccaaa
     8761 ggaataatca cgaaactagt gaatttggat actttgcgaa tcgaatctac aagttcagaa
     8821 cgaatagctt tgtgagtctt tgcctcgcta gtcaagcgga cagcataatt atctgcacct
     8881 actcggataa cttcagccat gacagaaccg cttgtcaaaa agcttccaga aagcaattca
     8941 gctccagttt ctttgacaat caaatcagat tcgcctgtca aaagagcttc atttgcttct
     9001 gcccgtccag atataacctg taaatcggct ggtatctgtt ctcctgctcc gatttttacc
     9061 aaatctccta ataccagctc tttagtagaa atcgattgtt ctttaccgtc ccggatcact
     9121 agtacttctg attgggaaac aatcgataat ttccgtacaa gatttcttgc atgtatttct
     9181 tgataaattc caataacgat attgacgaca ataattgcta aatacgccat gttggaatac
     9241 gcacctacaa ataacagaca aatcccaata ccaaagttca gaaaattgaa taacgtcaat
     9301 atattatcgc tgaatatttg ttttgtactt tttgatgcat cttcctggta gtcattttgc
     9361 tggccagaag ccattttctg aactacctct tgctgtgtaa gtccattcat tttctttttc
     9421 tccattttcc gccaactttc aaaaataatc acgtgtttaa gtctagcgtg ttccaagatt
     9481 aaaaacaact tgtttttata aaatttatag aaaaacacac gaatgatttt acaaaaaaga
     9541 tgccaacctt tttgtttgaa tcacccgtgt gctgttttta ttatcaatct atctttactt
     9601 atattgttcc tccaaacggc tcatccacca acctagtgct gtaccggcta taaaaagaac
     9661 aggacaagct agtaccccag caactcccaa tcccgcgtaa tcggtaggaa tgatcataat
     9721 cgttgaagca aaaactacac ctaaaatgaa atggaatagc ttgctatatg cctttgagaa
     9781 aatgtaatcc atgatttttg acaatccaag aacacaaaga atcccaccaa ttccgatcgg
     9841 cacaatcact tcaagtcgta cttctttaaa tccgtcagcc atcgctttat acaatcccat
     9901 atacaccaaa aaattagaag ggctcaatcc tgggacgatc atacccaaac caatcaatcc
     9961 gccggcaatc agccatgttc caaaattctg atcgacttgc ccgtcaaata attgagcgcc
    10021 gtataataaa aacaaataag ccagaacaaa agtaacaacc atgatgatca catcagaagt
    10081 atctcttcct tttttccctg cttctttcca taacgcagga acagttccag cgatacatcc
    10141 gacaaaaaac cataatatcg ttgtttcata cgcacctaat aaaaaactga ctaaaaaaga
    10201 aagtagaaag atgcctgtca ctccgcctag accaacaggc agaaaaaaca aaacattttc
    10261 tttgaaattt tttgtaaggt gggctaaaaa agaaatcagc cgctcataca tgccaaagac
    10321 agctgctaat gcaccaccgc tgactcctgg caagataaat cctgaaccga taaacattcc
    10381 cttcacaaac cgtaacatcc agtctttggt tgtgtacccc acttcttgat tttccatttt
    10441 ttgccttttt tccactattt gcgcactcct tctttcattt aattttattc atgattgaac
    10501 atttcctgct attttactta aagacttttt cataggtctc tattattttt tcattcattt
    10561 tgacaatatc aggataaaac atgttacctc cattatgata gagatagccg ccattgtaaa
    10621 gcaaggactg gatccgccaa tatcggtatt gtttcccttc ctcatttcct aaaagtgggc
    10681 tcagcacatc tttagaatac gcttctgcta attctactgt gttttctccg ccatgatttt
    10741 ttacataggc aatataatct ttcccaaaat tatatgcttg gacagctgtc cataaatcgc
    10801 atccttgttt ctttgcttct actatcattt ccgccaaata tttgacacct tgatcaatac
    10861 tgtcagctgg attatcaatg atctcagctt ctccatgcag actttccgag ctctgcatga
    10921 gatctgtccc ttttcctttt gattcggtca atattatcgc cttgatcaaa ttctcgtagt
    10981 tttccatatg ataatttttt gcagctgttg ttatttctat ttgatagctt tccactcgtt
    11041 ttaccgaacg ataagttctt cctcataaaa acaacaccgc accaataacc gtcagaatga
    11101 tcatgtaaaa aagaatacgg ataatagata atcgttttcg cttcatttca tcacctagtt
    11161 attttgacat ctttttttat cttactattt ctttatcttc ttggctattt cttccttgat
    11221 ttctgtgtac gccttaagaa aaaatgaatt ttcctgtatt ttttgacatc ttacgaaaat
    11281 gatcgttgtt tttcagtaaa aacaattgcc caatgactga tataatcgtt atactttaca
    11341 cagtaggtga ttatgtgaaa aaagaaaaga aatatgcaaa aatgccagat gggagcgaaa
    11401 tttactatga aaagagcggt caaggcttcc ctctcttttt actgcatggc aatgacggca
    11461 gcggccgctt tttttcagaa caggtccctg tactcgaacg ctactatacc gtgtatctag
    11521 tagatagccg aggacatggt cgttcgacaa acgaagcatc tatgttgaat tttcagttaa
    11581 tggccgagga tctgaatacg atcatgttgc tggaaaaaat cgatcaggca gattttcttg
    11641 gatttagtga tggagcaaat ctagcactgg tatttgccag cagcttccct aaaaaagttc
    11701 atcgattgat tttaaattct ggaaacacgc ttgttaaagg cgttcgtttt tctgccagag
    11761 tcatcagtaa tattcattat gcatgggtat ggttactctc tttatttcgc ccaagtcttc
    11821 gcaaaaatct tttagttatc aaacttttgc tgcatgacat cgggcttaca gaaaatgatt
    11881 taaaaaaaat caactccccc actttgatca tcgtgggaaa aaaagatgta atcaagctaa
    11941 aacattcttt gtatatagct aaaactatac caaaagcttc ctttgtgctt gtgaaagaac
    12001 aaggacatga attagctaga aaagatcctg aacgcttcaa tcgagaagtt cttcaatttc
    12061 tttctgaaac ataactatta ggaatagaag gtgatttatt tgttaaaaaa taccatacaa
    12121 tggctgaaaa accacctagg actgttcaaa acaatctttt tgatttctgt cattgtcatt
    12181 attgtcggtg aattgatgtc catcggtaaa actctatcta tccagcaatt aggagaaacg
    12241 tttgccacta tacctgtatg gaaaagcgga ctaatgctat tgattggtct tatctctgtg
    12301 ttacctatgg ttggttatga tattattttg aatcggatgc ttgaacaaaa gcagaatcct
    12361 cgttacctgt ttgagacaag ctggctgatc aatacgatca acaatattgc cggtttcggc
    12421 gggtttgtta gtgtggggct tcgctctgaa ctatatggac aaaagaaaga gagcagaaac
    12481 gtgatccaag cattatcgaa agttttcttg tttttgatgg ctggactttc tatttacagt
    12541 ctgctttctt ttttacttgt agtcttcacg ccagttgctc cttttttgaa gcaatattgg
    12601 atatggttga tcggcggcgg tctatatttt cctgtcgtct ttttatttac tactttcaaa
    12661 aagaaaggat tccttggtgg tacctcactt aaaactcaag gacagctatt gctagtttca
    12721 ttgttggaat ggaccggtgt cctgtttagt ttcctttctg ttggttattt aatggaaatc
    12781 cggattgatc tgtggcaaac aattccatta ttcattgctg cttcagtgat aggaatcgtg
    12841 tctatgattc caggagaaat tgggagtttc gatgtaatga tgattatcgg gttgtccgct
    12901 attggtgtgc caagagaaat agtcgttgtg tggatcttgc tctatcgcct gttctactac
    12961 atcattccgt ttttgattgg aatcgtcttt ttcttcaaaa atatcggttc cacttttgat
    13021 cagcgttatt ctggtatccc taaacaatta gcaacagaaa ttgcgcataa aatcgtggtt
    13081 gtcttattat acttttctgg aatcatgcta gtactttctg ccactatccc gcaagctttt
    13141 acagagtttc gctggcttca tagtttgaac ccattgaaat ttcattttat tatccaattt
    13201 cctagtatac tattgggatt tcttttaatc attatgggca gagggatcgc agcacgtgtg
    13261 aaacgagcat atttgcctac aatcttcttg attgttctag ctttacttta tgttttatta
    13321 agtgatttta gtttcacacc ggtggttttc ttgtctattc tgcttctgat cattcttgct
    13381 tctaaaaacg aattatttcg ggaacaattg atctattcat gggagtggcg tacgatcgat
    13441 ggaatcatta ttggtgcact gtccttgctt tacatcgtta ttggggtata caatctgccg
    13501 gatttccctc atcggcgtca ccattttatt tcctttttct tatttccttc tgaaaagatt
    13561 tggttttcgg gattgttggc catcatcgct gttagtttca tgatcgttct atttgttcat
    13621 ttccttcaag gagaaaagaa acagattggc gaagccttta atgaagaaaa ggccctgaag
    13681 atccttacaa cttatggagg aaactccgat agtcaattga tcttcttaaa agataagcgg
    13741 atgtttgcct atgaaaaaga cggtgaacca actgttcttc tccaattcgc ttgcttcaat
    13801 aataaatgta ttgtaatggg tgatccttct ggtaagaaag aagattttcc agaagcaatc
    13861 gaagctttta tcgaagaaac agatcgacta tgctatttac cagttttcta cgaaacaagc
    13921 gaagagattg ttatgatttt gcatgaattc ggctacgatt tcattaagat gggggaagaa
    13981 gcttatgtag acctgaatag ttttacaact tcagggaaaa aaatgaaggg cactcgtgct
    14041 gtcttgaatc ggatcgaacg agaaggattt acatttgatg tcttacaacc accattttct
    14101 actgagcaaa tgagtacttt caaaaatatc tctgataatt ggttgggttc acgaaaggaa
    14161 aaagggtttt ctttaggctt tttctctgaa gattatctac aaagagcgcc tattgcagtt
    14221 gtcaaaaata cccaagaaga aattgtcgct tttgcaacga tcatgcctac ttacacgaac
    14281 aataaagtag gaacgattga tctgatgcga tatgattcta aaaaagcacc ttctggcagt
    14341 atggatttct tgttcttgca tttgtttgcc tatatgaaag aagaacatat ccaatggttc
    14401 aatttaggga tggcccctct ttcaaatgtc ggaacttcca gaaagagttt tttacaggaa
    14461 aggattgcat acttggttta cgagtttggt tctcattttt attctttcca tggattaaga
    14521 gaatataaaa gcaagtacgc tacaaactgg gtctcacgtt atactctata ttctagagat
    14581 agttggattg catatgttat gattgctttg ctgattattg ataatgcgcc agttgagcag
    14641 acacaaggta gaataagtgg tttgaaaaaa tggatacgaa gaaagtatta aaaactgcat
    14701 accgatagat gaacactctc atgatgaata aaagagactg aagtaaaacg aaagttcatg
    14761 cttctacaat ctcttcttat acaattgaag aacaaagagc ggtccaaaca gaaatcctgt
    14821 ttggaccgct cttttatttt aaacattttt cattaccaaa atattcgagt cgcgtttctt
    14881 atgcttcgat ctctgtaacg atacctgaac ctactgtccg tccgccttca cgaattgaga
    14941 atgtcgttcc gttttcaaca gcgattggat ggatcaagtc aacatcaatc gtcacgttgt
    15001 caccaggcat gaccatttct gtatcttctg gcaatgtgat tgttcctgta acatcagttg
    15061 tgcggaagta gaactgtgga cgatagttgt tgaagaatgg agtatgacgt ccaccttctt
    15121 cttttgtcaa cacgtacact tctgctttga attttgtatg tggagtaatg gaacctggtt
    15181 tagcaagtac ttgtccacgt tcgatatcgt cacgttggat cccccgtaac aacacgccta
    15241 cgttatcccc agcttccccg taatctaacg ttttacggaa catttctaca cctgttacaa
    15301 ctgctttttg tgtttcaggt ttgatcccta cgatctctac ctcatcaccg acacgaacag
    15361 caccacgatc aatacgacct gaggcaacag ttcctcgacc tgtaattgag aagacgtctt
    15421 ccactggcaa tagtaatggt ttatctgtat cacgttctgg tgttgggata tattcatcta
    15481 ctgtatccat caattccata atagcagctt cagcatctgg atcgccttgc aatgctttta
    15541 atgcggaacc tttgatcaca ggagtgtcat cgcctgggaa accatattcg ctcaataact
    15601 cgcgaacttc catttctacc aaatcgatca attcttcatc atcgactaaa tcaactttgt
    15661 tcaagaaaac aatcaggtat tttacaccaa cttggcgtga caataaaatg tgttcacgtg
    15721 tttgaggcat cggaccgtca gttgcagaaa caactaagat tgcaccatcc atttgtgctg
    15781 cccctgtaat catgtttttc acatagtccg cgtgtcctgg tgcatcaata tgcgcgtagt
    15841 ggcgtttttc tgtttcatat tctacgtgtg cggtattgat cgtaatccca cgttcacgtt
    15901 cttctggagc tgcatcaata ctagcatagt cttgaggatt tgctaggcct tttttgccta
    15961 atactgtagt gatcgcagca gttaaagtag ttttcccatg gtcaacgtgt ccaatcgtac
    16021 caatgttaac gtgtggttta cttctgtcat aatgttcttt tgccataatt tacaaacctc
    16081 ctaaataata tagatcactg aaaaatttca gctctcacct ttttattata gtcttttttt
    16141 gactaaatca aaaagaatac gtccgactta ctttttgtaa gattcgaact aagcttatta
    16201 tacgtcaata ttggtcaaag tcaactaaga gcactcgtct gtcttatacg cgaaaaaagt
    16261 accttttata aataaaaaaa tgtgtttttt gagaaaaact tcaagatagt ccttaacttt
    16321 cttttgaagc aataagaaca aaatgaggaa aagtttaaaa ttcagctgtt gccaacaact
    16381 ttcaatagac gaaatcttct atttcgttaa taataaggaa agaggtgaag tacatgaaaa
    16441 aattaattgg cgtacttgtc gttttaggca tatttctcgg cggaagtttt gcagcttatc
    16501 attattttta cggcggcgaa gcttattata caaaaatcac gacgaatggc gaaatgtcag
    16561 ctacccaagc agataacgga gagaaattca cgacttatac ctacaagcaa gcggcttata
    16621 ataaagatgg tgaaaaaaaa caggtgacgc ttcgcgaaga aagagaacat ccattaaaga
    16681 tgaatgcgta tcttaaactc aaagtcaatc ctcgtaaagg tgtattaagc tgggaagaaa
    16741 taaaggcttc tgaagtgcct aaaaatgcgg cagaaaaaat cgataattag taaatggtct
    16801 taaaatttta ttagataaaa
//
LOCUS       DACECZ010000052        16802 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.052, whole genome shotgun sequence.
ACCESSION   DACECZ010000052 DACECZ010000000
VERSION     DACECZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.052"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(29..742)
                     /locus_tag="IWO35_001568"
     CDS             complement(29..742)
                     /locus_tag="IWO35_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0316629.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            917..2341
                     /locus_tag="IWO35_001569"
     CDS             917..2341
                     /locus_tag="IWO35_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0316630.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2640..3347
                     /locus_tag="IWO35_001570"
     CDS             2640..3347
                     /locus_tag="IWO35_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ0316631.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3334..3657
                     /locus_tag="IWO35_001571"
     CDS             3334..3657
                     /locus_tag="IWO35_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0316632.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3746..4783)
                     /locus_tag="IWO35_001572"
     CDS             complement(3746..4783)
                     /locus_tag="IWO35_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0316633.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5104..6087)
                     /locus_tag="IWO35_001573"
     CDS             complement(5104..6087)
                     /locus_tag="IWO35_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ0316634.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6502..7272)
                     /locus_tag="IWO35_001574"
     CDS             complement(6502..7272)
                     /locus_tag="IWO35_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0316635.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7265..8200)
                     /locus_tag="IWO35_001575"
     CDS             complement(7265..8200)
                     /locus_tag="IWO35_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0316636.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8516..10165)
                     /locus_tag="IWO35_001576"
     CDS             complement(8516..10165)
                     /locus_tag="IWO35_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0316637.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10282..12885)
                     /locus_tag="IWO35_001577"
     CDS             complement(10282..12885)
                     /locus_tag="IWO35_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0316638.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLILIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12897..13946)
                     /locus_tag="IWO35_001578"
     CDS             complement(12897..13946)
                     /locus_tag="IWO35_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAQ0316639.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13972..14286)
                     /locus_tag="IWO35_001579"
     CDS             complement(13972..14286)
                     /locus_tag="IWO35_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAQ0316640.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14302..14748)
                     /locus_tag="IWO35_001580"
     CDS             complement(14302..14748)
                     /locus_tag="IWO35_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0316641.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(14749..16725)
                     /locus_tag="IWO35_001581"
     CDS             complement(14749..16725)
                     /locus_tag="IWO35_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAQ0316642.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
BASE COUNT         4988 a         3414 c         2764 g         5636 t
ORIGIN      
        1 aagctctgaa aaaataattt tcccgagctt atttttcaac aaaaacagct tcaaaaacaa
       61 agtcttgata taaatgtctc gataccgaat actcgaaagg tttaccagac gatagagcaa
      121 caactttgtc tatctcaatc ataaattcac ctggatctat ttttaatatt tttgcgtcaa
      181 attcattcgc agtatctcct ttgatacgta tgtgcgaagt ctttatttcc agacccagtt
      241 catttgtgat ataagcatat acagatttct ttaaatgttt tggttccaag cctggtatga
      301 ctgctaatgg catgaatgtt tgctcaatcg agtatggttg acaatcaata aaacggcttc
      361 gtacaatatt atatacatag tcttttacac tgatacttaa tttatcagct acttttttcg
      421 atggaagttc aattgagaaa gtatggattt ctgaatctac gtgttcttca ccaaccacgc
      481 taaacaaacc ttcgtttgga ccaagaactc gtgagttatc aattggtgtt ctgacgactt
      541 cagtcccata cccacttcta cttcgcaaaa ctccttcgga aacaagtaag tctacgcctt
      601 tctttactgt taacttgctt gtatgataaa taactgccaa ttcatctcct gtaggaagtt
      661 tctcaccaat tttatactct ccatttacaa tttttttctt tatatctaag taaatttcca
      721 tatacttcgc aatttttttc aatggtgacc ctccaatcaa aaacatatct tcatctatac
      781 tttaacataa ataaaccgat aaaatccaca tcaaaattat acacacaata aaaaagatat
      841 atatatttaa acaaaactat ttactttaag ttgtaagcgg tgttatcatt aagtggaaac
      901 aggaggtaaa aaaattatga caactatgaa aaagctaact tttccagatg atttttggtg
      961 gggttccgct tggtcagctg agcaagcaga aggaaaagga gaaacaggaa aagcagagac
     1021 tatctgggat cgctggttta aagaacaacc ttaccggttt tatcaacgaa tcgattcctc
     1081 tattacaacc gatcacatcc atcaatataa agaagatata gaactaatga aaaaaactgg
     1141 gcataactcc tttcgcgttt ctatttcttg ggcacgaatg tttccaaatg gcggtaccgg
     1201 aaaagtaaat cccaaagcca tttctttcta tcgaaaatta ttttccgaaa tgaataataa
     1261 cggaatcaaa gtatttgcca acttatatca ttttgacatg cctgctactc ttcaagataa
     1321 aggaggatgg gaatcacgtg aagtagttga tgcttatgtt cattacgctg aaacttgttt
     1381 taaagaattt ggtgacttag tttgtcattg gtttaccttc aatgaaccac ttgggcctat
     1441 tttaggtact tatcttgaag attttcatta tccaaacctc attgacttta aacgcggagc
     1501 gcaagcagca ttcttcacta tacttgcaca cgcaaaagca atacaagtat ttaaagaact
     1561 agctcttcct agtaaaatag gcgttattct aaatttgagt cctacttttc ctcgtagtca
     1621 aaatcctgct gatgtgaaag cagcagaaat cgctgattta ttttatacgc ggagcttttt
     1681 agatcctatg gttaaaggga atttttctaa aaagcttatc ctgttattac aagaatataa
     1741 tcagcttcct gaagattata cgaaagaaga tcttgtatgt atctacgaaa ataccgctca
     1801 aattcttgga ctaaactatt acgagccacg tcgcgtcaaa tctcgttctt ctatggtgga
     1861 tacatctagt ccatttttac cagagtggtt tttcgaacca catatcatgc ctggacgacg
     1921 aatgaacgaa taccggggat gggaaattta cgaaaaagga atttatgatt tgtgcatgga
     1981 tatcaagaac cattatggaa acatcgaagc atttatctcg gagaacggaa tgggcgttgc
     2041 ggatgaagaa cgtttcatgg atgatacagg gcaaataatt gatgattatc ggattgagta
     2101 tatcaaagat catcttgctt acttatggaa ggcaataaat gaaggatgta acattaaggg
     2161 gtatcatctt tggacattta ttgattgctg gtcatggatc aacgcctata aaaaccgata
     2221 tgggctggtt tcactcgacc ttactactca aaaaagaaca attaaaaaaa gtggcgaatt
     2281 ttacaagcaa ttaagtgata caaatggatt tagatacgat gtagacttgt tagttgaata
     2341 atattctaaa attccacttg aagtgaacac attagaaaac aagaataatg agaaatacca
     2401 ataacaaata ctttttcttc atacttcatt tatacttgtc agtataagag actgtgacaa
     2461 atgtagtgat cctcagaagt taggtctgga aatctaactt gagaagattt ttttaaacta
     2521 cgtccagtct tttttatttg tttaaaattg aagttgacaa gtcttcatta aaaatttaga
     2581 atattcacaa gtttgacgac aattatgaaa atttatgtcg tgctaaagga gttttgttat
     2641 tggatgaaaa gttagatata aaaactgcga tcaaagacac gttacctacc gtttttggtt
     2701 atatcggtat tggacttgca tttggtatcg ttgggaaagc tgccgggttt catccgctgg
     2761 tcgtcacgtt gatgtccctg ctgatctatg ctggttctgc ccaatttatc acggtcagca
     2821 tgcttgctag ccatagtcca ctgctctcca tcgttctctc gacctttcta gtcaattccc
     2881 gaatgattct gatgagtatg acgattgctc cttatttcaa gaaaaatcgg ctgcttcaaa
     2941 atctgctgat tggcacattg ctgacagatg aaagtttcgc actgggaatg aataaattga
     3001 actatactgg acaaaagtta aactttcgtt ggatgaatac agctaattgg atttcttatt
     3061 tgacgtgggt gggttcttct cttgttggcg cacttttagg gaattttatt actgatccga
     3121 aaaagtttgg tttggatttc gccatcgtcg ctatgtttat cgggttactt tatcttcaag
     3181 tcatttctga tagaaacacc tctaaacgat tacagttgat cctgattggt ctcactttga
     3241 ttctggtata tgtcggactg atttttatcc caagtaactt agtgatttta gttgtaacac
     3301 tgattgggtg cggattgggg gtgtggatca aacatgcctt cttttgagtt tacattgctg
     3361 actattttag gttgtacttt agcaacttgg ctttctagga tcctaccatt tgtcttactg
     3421 aaaaaattcg atttgccgca accgttgctt gagtatttaa gttttgtccc gatcgtcatt
     3481 atgtctgctt tatggttcag cagtctattt acccaaaata tcggacatct gccgcagatc
     3541 aatctcgaaa atgctttggc ttcagttcct acacttttag ctgctattct ttcaaaaagc
     3601 ttattggtga tcgtcttggt aggaattctt tccctttcgt tgattcgctt attataaata
     3661 aaaaaacaag agcttatggc aaattactgt catgcgctct tgtttttatc aaagtcaaat
     3721 aagaaaaaaa tcggcgtatt cttgcttatc cgttgaacgg accactaaca acgacttttc
     3781 ctaatatatg tcctgtttct acgtctttgg ttgcttgttt caagttcttc gcattcatcc
     3841 catctgaata gaccttgctc aatgtactgc ggataatccc ttcatttgct aaagcagcaa
     3901 tgtgcgctaa agcttctcct tgcgtttcga tttcatagtt atagtctgtt ttagcgaaca
     3961 tatactccca gtcgaagctg atagattggt ttttccaaac acctatatct aacggctctt
     4021 tgatccctac gatcgttccc acatgtccaa atggtcggac aaggtgtttg atttgatcaa
     4081 agtagcgggt aatatcgaat aatacagcga catactctac ttgatcgatt cctaattcac
     4141 gaaaactttt acttaagtcg tgatgataat cgattggata gtctacacct gtttttttca
     4201 gccattcaaa attcttcgga cttgcagtcg cgtagacttc taggccagcc caatgcgcta
     4261 gttgattcaa aatcgaaccg acgcccccag ctccgttgat cactaagatt tttttcccgc
     4321 ggttggcatt ttcttctgga gtcagaccaa atttttcaaa taacaattca taagcggtca
     4381 atgaggtcaa cggcaaggct gcggcttctt catctgaaag attttttggt gcaagagcga
     4441 caatacgttc gtcaaccagt tggtattctt gattgcttcc tgctcttgtc gttgtacctg
     4501 cgtaaaaaac gcgatcgccg acagaaaatt tttgcacttc atcacctaca gcaacaactt
     4561 tcccgactcc atcaaaccct aagatacgta aagcatttct tatcccgtta tcttggcgaa
     4621 gtttcgtatc gacaggatta acagatactg cgttgacttt taccagaaga tcccgtgaac
     4681 tgggtacagg tatctgttct ttttcatcaa taaagctttt tggatcatcg atcgacaacc
     4741 cttcataaaa accgactacc ttcattaatt ccttttctga catacgaatt cctccttagc
     4801 ttcatcaagt tggagcatcc atacagtttt catttcaata tatatagtat gctatttgtt
     4861 ccacttttat tcatcataat tattctctta cttaaagtaa actatttgca cttttttggt
     4921 taaaaaagag gttgcgacaa gacttttgtc acaacctctt ttaaacggtg ttcaatcaac
     4981 tgaccctcgg tattcgctca aaaaacgaca aaatatgagg agctattttt gtttggctgt
     5041 tcttattact tgatgcagga cagctttttc acgacctcta attcacggtg ttcaagcaag
     5101 aaatcattgt tcactgaaaa gcgttggatg ttctttttca aaggtgtcta agatctctcg
     5161 tacttcatca gtagatttcg tattcatcaa ctgaactcgc aagtcatttg ctcctggaaa
     5221 tcctttgata tagattttaa agaaacgatg tagtcctgtg atggaacgcg ggatgatttc
     5281 tgaataatga tcttgcagat ctagttgcag acgaagtagt ccgatcagtt ctttttctgt
     5341 atgttccctc ggttcttttt caaatgcata aggattcttg aaaatccccc ggccaatcat
     5401 gatgccatct acgccatatt tttcggccaa ttcttctcct gtctggcgat ctgggatatc
     5461 tccgttgatc gtgatcagtg tttgcggagc gatttggtca cgtaaagcaa tgattttagg
     5521 gatgacttcc caatgtgcat ctactttact catctcttta cgcgtacgca aatggacaga
     5581 aagattggct atatcttgat gaagtaggtg actgatccat tcttccatct cagacatttc
     5641 cgtatagccg atacgcgtct tgacgctgac aggcaaaccg ccagctttag ccgcttcaat
     5701 caattgtgcc gctacttctg ggcgaaggat caagccactg ccttttccac gttctgctac
     5761 attagggact gggcagccca tgttgatatc gattccttgg aagcccattt ctgccatagc
     5821 aatgctcatt tctctaaaaa attctggctt gtctccccaa atatgggcaa ccatcggctg
     5881 ttcatcttct gtaaaggtca atcttcctcg tacactgtct tttccatctg gatgacagaa
     5941 actgtctgag tttgtaaact cagtaaagaa gacatccggt gctgctgcat gctttacgac
     6001 atggcgaaat acaacatctg ttacatcttc cattggtgct aaaacaaaaa aaggctttgg
     6061 cagctctttc caaaaattag tagtcattta tttgttactc tccattctat caagcagtga
     6121 agtcgcaatc tttctcaaaa acattgtcat tatactagga aacaaaaaaa gaagcaaagt
     6181 gaacagctta aaagatgttt ctgattgagc aaaaaaagac tgtgacaaaa atcttatcac
     6241 aattcctttt ccggaataaa tggtgttcaa aagcttgcat ctgcggtaat aagtccaacc
     6301 aaacgaaaaa taggaggagc tatttttgtt tggctgttct tattacttga tgcagaacag
     6361 ctttttcaca acctctccac taaacggtgt tcaatcagct gaccctcggt attagctcaa
     6421 caaacgacaa aatatgagga actattttcc cgtttgtttc tctaatactc aggtcaaaac
     6481 gctgatttca caacctcttt tctaacttct caatcctctt cctttattga tcaaatacca
     6541 agtaacggcg tacaaaacaa cgatgaatag cacaagtacg atcatcgatg ttgctactgg
     6601 cacatcgatt gttccaagga atccgtaacg gaaaccggag atcatataaa cgatcggatt
     6661 gactttcgaa acagcttgcc aaaatggcgg caacatagag atggcataga aaacgccacc
     6721 tagataagtt aacggctgca agacaaatgt aggcacgatc gatacatcgt cataagactg
     6781 agcaaaaatc ccgttgagca gtcctgctaa cgaaaacaag attgccgtca tcaataatgt
     6841 aatgatgacc atgaaccatg aatacacatg aagtggtaca aagaacaagg aaatgatcgt
     6901 tacgagagag ccgacgagaa tactgcgacc cactccgcca atcacaaagc cccaaatgat
     6961 cacatgcgta ggaacaggcg ctaccaagat ttcttcgatg tttttctgga atttttgaga
     7021 gaagaaggac gaagaaacgt tggcatacga actagtgata gcagacatca taatcaaacc
     7081 aggcacgatg aattccatat atgaaaaacc acccatgtca ccgatccgac caccgatcat
     7141 ttttccgaaa ataacaaagt acaaggatgt cgtgatcaca ggcggaacca gtgtttggac
     7201 ccagatacgc aaatagcggt tcgtttcctt gatagctaaa ctttttaatg cagtaaaata
     7261 aagattaaac atttttctcc tccgtatgat tattttcttc tgtgattttc aaaaacagtt
     7321 cttctaaacg attcgactta ttcctcatag aaagaacttt gattccttgt cttgtcagct
     7381 gatcgaagat tccgttcacc ccttgatttc gttcaacttc gacagcgatt gtctgttcat
     7441 cttcaaatgt acaagcgtat ccttcaatca caggtttggt ctcatatggc gctaaatcaa
     7501 agataaatgt ttcaaattgc agtttggata gaagctcttt catactagta ttctcgatca
     7561 gttcacctga ttggatgatc ccgatgttgc gacaaagcat ttcagcttct tctaaataat
     7621 gcgtcgtcaa aatgatcgtt gtaccatttt cgttcaattc tcggagaaac tcccacatct
     7681 cgcgtctcaa ttcaatatcg actcctgctg ttggttcatc caagatcagc aaacgtggtt
     7741 catgcatcaa tgccctagca atcatcagtc ttcgcttcat tcccccggaa agcatacgtg
     7801 cacggacatt tctcttttcc caaagattgg actgcttcaa atatttttcg ctgcgtttca
     7861 tcgcttcttt gcgagagaca ccgtagtagc ctgcttgatt cacgacgatt tgctggaccg
     7921 tttcaaaggg attgaaattg aactcttgcg ggactaatcc aatctgttgc tttgctcgga
     7981 ctagatccgt atccagatca taaccgaaga cattcacttt tccagatgtt ttattgacta
     8041 aagaagtaat gattccgatc gttgtagact tcccagctcc atttggtcct aatagcgcat
     8101 agaaatctcc ttctttcacg gaaagatcga ttcctcgcaa tgcttcgacc ccacttccat
     8161 aaaccttttt caaattattt atttcaagcg catttgccat ttatttccta tctccttata
     8221 gacatgattc tttattgtat catggaatac agcagatgaa aaaacaactt ggttccagcg
     8281 aaaaatcagg ttgctgggct caggaaaatc tcctaatgaa agacatcaat cggacgaagt
     8341 cagacaacct tttcagaaaa aacaaatcga agataaagga ttactattca tatgtgcaac
     8401 aaaaggagcg tgagacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa
     8461 cggcggaagc aaaagccaag atttttgatc caataaatac tttcgatcac ttcgcctatc
     8521 ccaatacatg ccactgccgc ttttcatcaa ataacgcggc ttccgttttt gcggtttcta
     8581 ctaaagtctc tgggtaatcc aaaactttca gcaaggcaat cgcatttcgg gtccttgaag
     8641 gtccttcttt taatttgaaa tcaaaactga ttccttttcc ttctgttact tgttcttcaa
     8701 agtggacatt ctcacaataa ttctttaaaa tctctgtcaa ttcgatatca tgtgtggcga
     8761 caaatgctaa agatggatac tccgcaagcc accgaacgac tgaagcggaa gaagcaatcc
     8821 gttcgatcgt atttgtccct tttaaaatct catctacgaa gcagtagcag cgttctttgg
     8881 aagccacttg gtccaacaaa cgtttgatcg atttgatttc tgaaacaaaa taactgtcgc
     8941 cttcaaataa atcatcttcg attgccatgg aagtcaacac atgtccaggt tgcagggtaa
     9001 actgttctgc aatcgctgtt tggatcgtct gtgctagaat acagctgatg gctatacttt
     9061 tgacatacgt agattttcct gatgcattgg aacccgtgac taatgtattc ttctgccagt
     9121 tgaccggatt cactaccgct tcttttaaca atggatgata gatatctttc gctgtcacac
     9181 cgccctcagt aaactctggt cggcaagtga tgggcatata agtacggtaa ttcaatattg
     9241 cagcggctac ttctaattct cccagcaatt cccacagctt gatggcttca gttgaatgct
     9301 tttctaattt agtgagaacg aaatgatagg agataaatgg cagcatgaac atgatgttga
     9361 catattcaaa aatcaactct gcttcactcc cactcttcat acgaaatgaa aaacctaatt
     9421 tagggatgtc tcttaatggt ttagtttgct gtttcagctc ttcttgaagt ggtgtcggca
     9481 gttttgctag ctgattggcg gctgaaatcg ttgaaaccaa ataactcatg ctattcagct
     9541 ctgtttctag tgtttgtttc ttcactagat aatagaccgt attgaatact aacccaccga
     9601 ttgccaaata gattcctagt acttgtccta aaagaagcaa acccaaaccc accagcggaa
     9661 gcaagccaag aaaaataaat aaacctacgc gtccaatctt tttacttttt ccatccgcta
     9721 gatattgttt tgcaaaatta tgatcttttt ttcctaaacg agcgaattga tattggattt
     9781 tttctctcag ttgaggattt tcctgataaa aagcaatcag tttttctaac tgctgatctt
     9841 caccaaaatc aaagctgcgt aaacgttgat acaacgcttc tgaacctaca ctagaatagg
     9901 tactgttgat tccttcgaat acttcgaaca tatccaggtc ataccaagta agatcatcga
     9961 tttcgctatc ccatgaacgg aacttcttct ccgtgagcca tgcttccttc aagctttctt
    10021 ctttatcaaa tctcggttga taaggaattt tttcccattt gctacgaact gtttccttta
    10081 atttcaaacg tgaaaaaatg tctaaagcaa ctaataaaat aacagctgca ataataatta
    10141 aaacgataaa ttctctttga cccatatccc tctccgttcc tttatttgtt agctttagta
    10201 tagcactgtt gcgctatttg aaaagagggt aaaaaaacac ctttcaaaat aaaaggtgtt
    10261 tttttcttgt atattacata tttatctctc atttatcaaa attgacaaat aatcataggg
    10321 aggaattgtt gttaattctt gtttattctc cagtgcattg acaactgtcc caagggaaag
    10381 gatagcctca ggtactattt tttcttgttc tgttggatta gctaagcgaa gaacaaatcc
    10441 ttgttcatga aaataagaag gataaatcga tgatacaatt aagtcttcag gtaattcaat
    10501 caatgaaaat ttacgaggaa catcaagttc attttgtaat ggccaaatct tgttatctaa
    10561 cctgtgaata aatacattca gtttctgctt ttgataagaa atagattgtt ctaaccgttt
    10621 ttctgcctgt tttgccactt tgaactcatt aaatcttcct tcttctacat taaatccaaa
    10681 agtgacttta tactcaccta tctcttgagc tagcggtgtt tccatcatga catgcccttc
    10741 atttgtcgta tctcctgaag ctcgcccagg tcgccaagca agattaggtt ttccaagttc
    10801 ccctgttgtt gaaaataagg ttatgattaa ttgattttct atacgttcat actcttttaa
    10861 accatctgca taaaacgtaa aataatgatc attttctgcc acagagacac tcttatcgaa
    10921 tatttctaaa ttaacaggtt tctctaccat agtttctgac caattcggag gaatgacatc
    10981 aggggtattt tcaataaatc cgttttgaat ttgtgcaatt gatttttcag atttatctaa
    11041 aacattaatt gctaagcgca tacgatgcga gtaaacagta ttatgaattt tgagtgtgcc
    11101 ttcaacgatt tgactttctc ccagactgaa cgatatttca taagatattt cttccatttc
    11161 gggtttttca gataaacgat cttttaaatc tttaggaagt tgagcttttc cgtaaacaac
    11221 taatgtttct tgtaaagaat ctttatatac tttagctgtt tctaatttta gagatagttc
    11281 tgtatctcct tctaatggtg aataatcata cgtatcgcca tcatttgctg aatcgatcaa
    11341 atgaacgaaa tcaagatatt gtctatctcc aactatcaaa ttcaattgcc catctttaaa
    11401 agaaagttta tatttattgt tacttatctc agttttgtct atctcgattg ctgtttctaa
    11461 tttttcatta tttttctcaa attcaacaac agtgtatcct agtgctggca catttacatt
    11521 tatttttatg tcaagttcat aataagctgg ttcatcaaaa tattcgaaac ctgtagcaat
    11581 catccgataa gcatttttcc gttctggaaa ataaacttct ttttcaatga tattataggg
    11641 actacctttg aagtgtattt tctttgaatc agctactaca tgcacgattt tcgatccact
    11701 ataattctca gtcgttgtgt tgaaaacaag gacttcattc gattttagct ttaatgcatc
    11761 agccatccgt tttacaataa ggttttcgat tccgtcagct atctcattag cttctttgat
    11821 tcgatggaaa atatcttccg caacattatc tgaaacacag ccaccaatac tatcatgagc
    11881 ctggttttgc aaaatctttt tccataaccg acgaagtagg cctttactga tatggatacc
    11941 atttctttct gcaatcagta tgagcggctc aactcgtttt agaatttttt gttccaattt
    12001 aaaattttct attttcattt ttgttcttac tgagccaata gaacgatgaa cacgagagta
    12061 aaccggctga cgaagttcac ctttatattc aggtaattct tttttgtttc tgataacttc
    12121 tacataagaa ggataatctg aaataatatt gtggaattta ctgtatttat tcaacttctc
    12181 tacgacttga tcaaaattta aaatcattga cttttgatct acacccgaag gtactaaaat
    12241 atctttgttt tttccttgca tatgaagcat ttcaactgct tcatccaact tttcttttgc
    12301 aaattcagga atatctttaa ttgcgtctaa ggtcatcatt ccagtcatat aaccaaatgg
    12361 aaaattcatg gcataaacaa atttatctcc caatcctttc cagataaaat aaggagatgg
    12421 aaccatcgtt tcaaagttta atcctcgcca acacatgaaa ttatcgattt ctacttgatg
    12481 caaaagtgta ggcaaattcg cattgaaccc aaacgtgtca ggcaaatacc ctaccatcat
    12541 tggatctcca tatttaagga tcgtatcact gattccaatc attaagtttc ttaaaattga
    12601 ttcagcatcc actaataagg catctgtttg tgcataccac ggtcccacga atagttgtcc
    12661 tgttttcatc agtcgttgga taaccttaag cttttgcgga ttgatctcca aatactcatc
    12721 gacgattgaa gtttgtccat ctaaacaaaa gtttacttta gggttccttt ccaattcatc
    12781 tagaacttca gtaaagacct gatcagacaa tacgatggca tcttcatttg taaaatacca
    12841 ttctctgtcc cagtgcgtgt gttgaattat atgtgcattt accatatcag attctcctac
    12901 tttccttctt ctactgaatt tacttgttgt tcttttttat acttgatgga ttttagtgca
    12961 ttgaccatta acgctgtaac cactccccca ataacaattg atactaaata taaaggtata
    13021 ttcgtaacaa aaggaattgc cataaaggtt gaaagcattg tttcttgagt aacaccaaaa
    13081 aatccattta gaccacatgc tactgcagtg cccaccacga tgcttggtat tactcgtaaa
    13141 ggatctttca cggcaaacgg gatcgtccct tcagtaatcc ccacaaggcc cataatcata
    13201 gctgtctttg cattatctcg ttccgattca tcataaagtt ttttgtttaa aattgttgct
    13261 aaaaacattc caaatggcgg aatagcaatt cctaataaca tggctgtact aggtccaaag
    13321 cttccgcttg aaaaagaagc aaccccaaaa gcatatgcga ttttattgat aggtcctccc
    13381 atatcaaacg ccatcattcc tcctaaaata gccgacatca aagcagcatt tgctcctgac
    13441 atgccttcta gccaattagt cagtcctgtt gtcattgctt gaataggcgt agctagtaca
    13501 taccataata tgattccaac aactaatgta gtaacaaaag gaatcagcat aattgatttt
    13561 agactaataa cttgcatcgg tacttttact tttttcaatg cattagctaa atagccacaa
    13621 gctaatccag caaccattcc tccaataaat cccataccaa gattttgaca catcatccca
    13681 gtgataaaag ctggtgctag accgggacgg tcagcaattg aatatgcgat ataggctgaa
    13741 atcatcggta cgataaactg taatcccgtc tgaccaatca ttcgcaggac tccccagaat
    13801 gtatccgtct ggtcccaaac acctgtgcca ccaaaaatga ctgcaataga agttagtaac
    13861 cctgctgcta cgacaagcgg gatcatatag gaaaccccac tttggaaatg atttagaatt
    13921 tctcttcctt gtttcttgaa ttccattagt aactccccca cgatatattt tttatttgtt
    13981 aattaatgca attgcttcat caatcgtctc ttcaccatct tttactacct ctgaggtccc
    14041 tactgttaaa atcggtttac cgtcaaagcg acctcgttct gaaacatttg tatctactgc
    14101 aaatatgaca acatctgcct cggaaacatc cgaaattgaa agtttgtttt ctatccccat
    14161 agccccctgt gtttctactt taatatctaa ccctttcttc ttcgcaaact tttctaaatt
    14221 agcttttgcc atataagtat gggcaatccc tgcgatacat gctgttactg ctactatttt
    14281 tgtcatatga aattcctcct tttaaaatga tagagctttt accaattctt ttttatttct
    14341 cgaatctttt attcttttaa caaactcgtc atccatcaat cttccagata attctgctaa
    14401 aatacgtaaa tgattagtac ctttatcagt gtcttttatt gctaacaaaa agacgagatc
    14461 aactggttga tcatctaatg cgccccattc aacagaccgc tttaacttgg ctacagccac
    14521 tgttgattca attactgcta ggcttttgcc atgaggaatg gcgatatttt tcccaattcc
    14581 tgtggtcata tggttttctc tgtcaaatac gtctttttga aattgtttgt ggtcagacac
    14641 aaacccatta gtctctaaca atttcgataa tttttcgact acactttttt tatcagatgc
    14701 atttaaatct atcactatat tatttatatt gattttttca ctgatcattt ataagccttc
    14761 tttcaatata taaactaatt tttcaaacga cgaactcgtt tttaaatttg ctaataactc
    14821 atgattcgaa gcgatttcat tgaatgtttc ataaatttta ttcaattcag gttttacttt
    14881 ttcattaagt gcaaaaaaga aaacagaatg aacaagtgtc ccatcccaat caattccttc
    14941 tggattaacg tagacacaga ttactggctt ttttataaaa tctacttcga tatgcggcat
    15001 agcaaaatca ttcatcgctg ttgaagatag tttttctctt ttgattgctc cctctagtat
    15061 cccttctttc gcgtaatttt cttttaccaa ttttttggta attatattta aaacgttcac
    15121 ttgattatcc tttatttggt gggtaaaaaa gcaaaatcgt ttatctaata aatttagaaa
    15181 agcatcttct gtttgttcat ttaactgtag atactgtttt acttttctga catctgcccc
    15241 taacatcaaa ggactaacaa caataacagg aaaatttgta ttttcaatcg gaatcgtgct
    15301 tataacaagt tcatcagtga cttttctatc ttgtagttca ttgatgctta gtacatcttc
    15361 tatctcaacc atatctgcaa aaccgttatt tattctttgc tctaataatt ttgatgttcc
    15421 atatccgcta ctacaaacta atatcacttt cgttttgtca ggtttgtaac gatccaaaag
    15481 tgattgaata tggagtgtta cgtaagcgat ttcatcttca ttaaaatgta tacagaatct
    15541 ctcttcgatt tcctctaata aatctacact tatcataaat gaccttttaa aagaatattt
    15601 gattttgtcc gtataagggt tatagatatt tactcccaat ttgagtcttt taactgctgc
    15661 attgagatgt aatactagat tattgatcaa ttcttcatca ggataaaatt catttaattt
    15721 aattcttact aactcttgta gctcaacacc catatctaaa taggaaagct tcgtttgatc
    15781 taagtcgtga ttcatagcat ttttttcaat cgaagtaatg tgaatgtcaa cataagcttg
    15841 ttcatctatt ggcacaataa ttgaaaatgc tttttcaatc attttgatta aaataagtgt
    15901 attctttgag atttcttctt cagaattatt actacgtgat aagaaattgc ttgtttcaat
    15961 cctttctatc gcaatagcaa ggtggatagc taaagtttga aattcataat ctgtaaattt
    16021 caattgtgtc ttattcatgt atcgattaag caattcggct acttttttca atatgtcctt
    16081 atttaagaaa ttattcgctt caccaatgaa attgatattc aagtctaaga gatctgtttt
    16141 actgttagat gctgtaaagc caccccaata atagttcaca acttctgaca tcaaccttct
    16201 tttacttttt tcatcagctt tgatgcgcac gccatttcga tctgaaacta aggacatccc
    16261 atgcttgttt agtctttttc taacatcttt cattgtgctt tctaccgtcg acctgctcac
    16321 gtataattcg tcagcaagat tttgaatctt tagagaatca ttagtattca ataatttagc
    16381 aaacacataa gttagacggt cctccggaga gtctgcgata ttgattttat gactggataa
    16441 taaatttcga agtgtttctt tttttccaat aagcgaaacg ccttttttag gttgaaccaa
    16501 catttgtaca tctggcattt tttggattat ttctttcaaa attaaaatat ctttgctaat
    16561 tgttttcgta gatacatata attgttctga taattctttg taagttgtgt gctcattttc
    16621 aatcaaaaag tatagtattt tcatttgccg ttcgctaaat tcgatgccca ttatcttcac
    16681 ctcctgaata cagcttaagt gtacaattat tatctctatt ttcatacaga agaaagttct
    16741 tgattaatgt agaagtttct aatattcttg attatttcaa atcaataaaa aagcctgcaa
    16801 ta
//
LOCUS       DACECZ010000053        16518 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.053, whole genome shotgun sequence.
ACCESSION   DACECZ010000053 DACECZ010000000
VERSION     DACECZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.053"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            92..1519
                     /locus_tag="IWO35_001582"
     CDS             92..1519
                     /locus_tag="IWO35_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0316643.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1611..1976)
                     /locus_tag="IWO35_001583"
     CDS             complement(1611..1976)
                     /locus_tag="IWO35_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0316644.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1973..2338)
                     /locus_tag="IWO35_001584"
     CDS             complement(1973..2338)
                     /locus_tag="IWO35_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0316645.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2530..2778)
                     /locus_tag="IWO35_001585"
     CDS             complement(2530..2778)
                     /locus_tag="IWO35_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316646.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2777..2905
                     /locus_tag="IWO35_001586"
     CDS             2777..2905
                     /locus_tag="IWO35_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ0316647.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2962..4656
                     /locus_tag="IWO35_001587"
     CDS             2962..4656
                     /locus_tag="IWO35_001587"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ0316648.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4756..4929
                     /locus_tag="IWO35_001588"
     CDS             4756..4929
                     /locus_tag="IWO35_001588"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316649.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5307..6440
                     /locus_tag="IWO35_001589"
     CDS             5307..6440
                     /locus_tag="IWO35_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ0316650.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6471..6791
                     /locus_tag="IWO35_001590"
     CDS             6471..6791
                     /locus_tag="IWO35_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316651.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6820..7284)
                     /locus_tag="IWO35_001591"
     CDS             complement(6820..7284)
                     /locus_tag="IWO35_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0316652.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7416..8669)
                     /locus_tag="IWO35_001592"
     CDS             complement(7416..8669)
                     /locus_tag="IWO35_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0316653.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8698..9015)
                     /locus_tag="IWO35_001593"
     CDS             complement(8698..9015)
                     /locus_tag="IWO35_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0316654.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9041..9361)
                     /locus_tag="IWO35_001594"
     CDS             complement(9041..9361)
                     /locus_tag="IWO35_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0316655.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9825..11240)
                     /locus_tag="IWO35_001595"
     CDS             complement(9825..11240)
                     /locus_tag="IWO35_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0316656.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11257..12573)
                     /locus_tag="IWO35_001596"
     CDS             complement(11257..12573)
                     /locus_tag="IWO35_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0316657.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12723..13421)
                     /locus_tag="IWO35_001597"
     CDS             complement(12723..13421)
                     /locus_tag="IWO35_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ0316658.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13710..14699)
                     /locus_tag="IWO35_001598"
     CDS             complement(13710..14699)
                     /locus_tag="IWO35_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAQ0316659.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14779..15612)
                     /gene="msrA"
                     /locus_tag="IWO35_001599"
     CDS             complement(14779..15612)
                     /gene="msrA"
                     /locus_tag="IWO35_001599"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0316660.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15787..>16518)
                     /locus_tag="IWO35_001600"
     CDS             complement(15787..>16518)
                     /locus_tag="IWO35_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316661.1"
                     /translation="TNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAKLLQFPWRILSL
                     QIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFNQKIISQPGHIV
                     VNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIY
                     RVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYTV
                     TTIISYLVTLTAYLCLFFKIVFHKR"
BASE COUNT         5154 a         3316 c         2801 g         5247 t
ORIGIN      
        1 ctttttcaga aattaactga tgatttcaaa aagaaaacat gtcataatat ttcccatatt
       61 gtgcaaaaaa atacaaggcg gaggctctac tatgattgaa gaatatattg agaaaaatat
      121 tttaagacag ctgtttttat gtgggcaatt ctatgtaaat aaagaagtca atctagaaaa
      181 gctctccaat ctcctgcatg tttgcaaaac cactttactc aatgatatca ataacataaa
      241 aaaagaattt gaagaacaga tcgcttatac acatcgggaa aaggaccgtt acactttata
      301 tttttcagaa catatacctc gatgcaagat catgcagcaa ttgtctcaaa attcgttgtt
      361 tttaaaaact tgtctgcttt atttggaaga agatgaacct gattatcttc aattaactga
      421 atgtgaattc atctctgtca gcaaagctta ttcattaaaa aaacaagtat tagcttactt
      481 taatgactgc gggatcgaaa tcgaccgcta ctcccctcga ttcactgaaa tggaaagacg
      541 tctgctgctt cttaatgtgt cgtatcgttt aggcggtttt aattcttggg agctacctga
      601 atcgtttttt gaacgtgccg atcgtttcat tgaatccgta acggaaaata gtggcagatt
      661 ttatgataaa gaaaataaag aaatcttatc gattggtttt gctatcagtt tcctacgcca
      721 gcaagtgtgt gctgtgacaa tagattcaaa atttatcgaa gaaatcaaaa aacgtcctat
      781 ttacaattat gtcgaatcag catgggaaaa cactgatttc caaacctatt ataaaaaaga
      841 agaattcgcc tttattttga cactattcaa tctttgtaat tatggtttcc attcttatca
      901 gttgattgcg gaagactttc aacagctaca ccaagtgttt attgacaata ctcccgaaat
      961 aaaagaacta gttgctacct ttgaatctca cttcaatcaa gaattattcg gaaaccaacc
     1021 gttcgaacgt gcgctgattc atctgatgcg ttcggcttgg gataactatc aattattcat
     1081 gccagaaaaa ttttatcttt taaatgagga acagacaaat ttatataaag aagtccagac
     1141 aattttttcc agctggtcca gccagttacc ttatgatcta cggttgaatc ctaattgcat
     1201 gcgggctttt gtgatcgaat tgagcggaat tctccgtctg acaaaagaac atctgactat
     1261 ctatatcgtc acaaattcgg atgttcatta tctgatttat cgggaagcct tggaagctgt
     1321 aactactttc gattttcaag ttgcaccaac aatttattca agtatatctg atataaagaa
     1381 gtatgcgcag cagtcctcaa accgggtatt atgtgaacgt acattatata caccagatgc
     1441 ggtccaatat gaaaatatta ttcctatttc gatcaatacg atcgatcgag ctatcatttc
     1501 agctgtgcaa aacaaataag gggctgtgac aaaaatcatg tcacagcctc ttatttgttg
     1561 aagatgccca tttctgtaac aatctcttga tacacgtcag gctcacaacc tcacttattt
     1621 tggacatcta ggggtccgat ctctttattt aaaaaataag aaatgatggt tcggataact
     1681 accaacgccg ctagttggaa aatatcctgc aaggtaggtt tgataatcga ttcgatgatc
     1741 ccagctgaaa caagaatttc caaacttaga agtatatagc ctcccaacat ctttttaata
     1801 gcattgttct cgcttactcg gtcgctttgc acttttgtat ggacaaataa atatttcaaa
     1861 aaatccttga ttgatagaca aacaccccaa accaataaac agatagcaaa gatatcaagt
     1921 cccagtacaa gtttttcgaa atatggaaga agaaactgca tcaactctgt cgtcatccta
     1981 cctctcctct tctttgtttt tctttgtctt ctatttcctg attcaagaaa taagaaatga
     2041 ctgttcgaat gataataata agtgcaagct tcaaaatatc tgtccaagtc ggcttgataa
     2101 ctgactcgat gatatcagca acgactagta cttccaagct taacaaaata taacttgcta
     2161 gcagttttct tgtatctgca ttacgagtat tacgttctgc atagtttttc ttgacatgag
     2221 accaatcaaa aagtttgaat agaacaagaa cgatcccgac aattaagata ccgatagaac
     2281 aaatatttaa aatagcaatg atccattcta gaaattcaat taaatttgag cgaaacatat
     2341 acgctaccta ctttctttta atttgttcct attcttttat tgtaaaagaa agttaccttg
     2401 ttcgtctatt taaaagttgt tctaatgata acggttcgct taaatttttc tttttcactc
     2461 tcccattttt ctccctccat ccataaatcg tggaagtatc caaaacaacg tagcaagtta
     2521 aaataaggct tagtccgtca tcagcttttc aaagaagcac tgtctcggtg ctttttttgt
     2581 acccaaattt ctaaaaatag aaccatacac ctcctcaaat cttttcttct tacctaccct
     2641 atttttattt acaggaaaat cagacacttc ttcttttttg tcaaaatttc ctttttcttc
     2701 tcgtcctatc atttggcatt ctaataaaaa cgaactatcc ttggatatag aaagagaaaa
     2761 caaggaggcg tttttcatga gtaaaaaatt aatcaaagtc ggcatcggtt taggtttgct
     2821 tgcccttggc gctgcatatc taggaaaaaa gacaggacta ttcgaagatg acagtcattt
     2881 atatgatgaa ttcgaatcaa tctagcatcc tatttagttg aatcactacg gacatacttg
     2941 gaagacattg gaggaatcac aatgagaaaa agtaaagcaa taatgattgg tgccggtatt
     3001 gccaatatgg cagcggctgt ttatttgatt caagaaggca aatggaaagg ggatcagatc
     3061 accttttatt cattggatga tcacggttca aatgatggtg caccgaccga cactgtagtg
     3121 gatgaatatt ggaataaaaa ccatccatta gaaaaccaaa aaggctatat tgctcgaggc
     3181 ggtcgcatgc ttaattaccg tacctatgta gatttgatgg atctgttaag tcgaatacct
     3241 tctgttactg agccagggat gactgctgaa gaagatacga gagattttga tgcaaaacac
     3301 cgaacctttg acaaagcacg tttgatggaa ggtggcaaag gaatcattga tggcggaaaa
     3361 ctgggattca ataataaaga ccgtattctt ctgactaaac tgattgctat gcctgattca
     3421 gaagaagaaa aactagacaa tatcactatc gaagaatatt tcaaagatga tccacacttt
     3481 tttgaaacaa acttctggtt tatgtgggaa acaacatttg ctttcagaac acgtagttct
     3541 gctcaagaat tgcgtcgcta tatgcatcaa atgatttatg aattcactca aatcgaacat
     3601 ctagtgggcg tcaatcgaac acgttacaat caatacgaaa gtattatgct tcctttgatc
     3661 gagtacttga aagaacaagg atgcaaaatc attttgaacc gccgtgtcac tgactgggaa
     3721 ttcaaagaaa caccgatgca agatgaaatc actgtgactg gtttgaaaat taccaataca
     3781 ctgactcaag aagaagaata cgttgtggtc gatgaagata cagcagttat tttcactaat
     3841 gggtccatta cagattcagc tactttagga gattacgaaa caccagctgt agaaaatatg
     3901 gattatggtg cagcttctag tctctggaaa aaagccagtg aacgtttcta caacttggga
     3961 aatcctgaca aattctttgc tgatcgagat gcaagcgaat gggtcagctt taccctgacg
     4021 acgaaaaacc atttgttgtt gaatgaaatc acccgtatca cgactcaaga accaggaaat
     4081 gccttgaatt catttatttc aaccacaccg atcacgccgt taggccaaaa agatgtcaat
     4141 atgtctattg tcgtgcatca tcaaccgcat tttacgtctc agaaaccaaa tgaaacagtt
     4201 atttggggtt atttccttta tccaagacga cgtggcgaat ttgtggataa acagtatatt
     4261 aaaatgaatg gaaaagaaat gctagaagaa ctgattggac aactttcaaa agtagatcct
     4321 ggaccagtga atattcgtga aaaagaaaca gaaattttcg acagcgtaat aaataatatc
     4381 cctgtctata tgccttatgc ttccgcgttg ttcaataatc gtgccaaaag cgatcgacca
     4441 aaagtcattc ctaaacattc gaccaatctg gcctttacag gagaatttgt cgagcagcct
     4501 tatcagatga tctttactga acaaagtgct gtacgttctg gagaaatcgc tgctttccat
     4561 tttgcgggta ttcctatgtc ccgtcttgtc aaaacaccgc gttacgacaa agatattcct
     4621 actctagctc gtgccgcaaa aaaaatgttt gaataatcaa gaggttgtga aaaagctgcc
     4681 ctgcatcaag taataagaac aaccaggcta aaacagcttc ttatgttttt gatttggttg
     4741 gacttattac cgcagatgca agcttttgaa caccgtttac caagaggtta tgaaaaagct
     4801 gccctgcatc aagtaataag agcagccaag ctaaaacagc ttcttatgtt tttgacttgg
     4861 ttagacttat taccgcagat gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa
     4921 ggcgtttagt ttcgagtatg aagaaaaatt ttgtgaaaat ggttcctcac gtttttgcat
     4981 aaatttgact taataccgag aaactgatcc cttctgtcat aacctctttt tatttatgtc
     5041 tgtttctttt tgaaaacaac tattttttca acgtttactc tcttgccaat ccaacatcat
     5101 ctaacggata caactatttc aatgattacc attacactaa aaaatcggaa acacacctct
     5161 ccgaacacaa atacataaga ctatagaatg tttcatttaa gaaaaaaagc gtttcattat
     5221 caaaaaggta agatctcctt taaaatgaaa ttatctgtag tactaataat gcagacaatt
     5281 ttatgaatct gaaaggagca tctccaatga aagcagtcac ttggcaagga aaacagaaaa
     5341 tggaaatcag aaacgtagat gatccaaaaa tattagaacc aacagatgca atcatccgta
     5401 tcacagcaac agccatttgc ggctccgatc tgcatcttta ccatcatggt gaagcagttt
     5461 tggaaaaagg gtatgttgta ggacacgaac caatgggaat cgttgaagaa gtgggatctc
     5521 aagttcaaac attgaaaaaa gggcaacgtg tggttatccc ttttaatatt agttgtggtc
     5581 attgtcattt ctgcctgaat cacatggaaa gccaatgtga tgaatcaaat ccagatcagt
     5641 tatatggcgg gcttttcgga tttggcaagt tgaacggtaa tcattggggt ggtcaagcgg
     5701 aatatctaag agttccttat gcagactcta cttcatttgt tgtaccagat aatgatcttc
     5761 ctgatgaaaa agttttattt ctatcagata ttttgcccac agcttattgg agcgttgaaa
     5821 acgcaggagt aaaaaaaggc gatacagttg tgattctagg ttctggtcca gtcggtctat
     5881 tcgctcaaaa attcgctgtt atggctggag cagagcgcgt aatcgcagta gatccagtac
     5941 agcaccgtct agataaagcg gctaattaca atggggttga aacttatttc ttagaagaca
     6001 cggcacaagc tggaacagaa ttgtatgaac tggctaaagg tggcgcagat gtcattatcg
     6061 attgtgtagg aatggacgga ttagagcctg tcaaagaaaa agcgaaaaat ttagtaagct
     6121 tacaaagcgg cacaatctct ccattacaga tggcatcgca agctgtcaaa aaatttggaa
     6181 cagtccaagt aactggtgtt tatatgactc cagcttcttc ttacccgctg caagaatttt
     6241 ttatgcggga tatcgctgtc aagcatggac aggcaccaat tatccatcta atgccaaaaa
     6301 tttacgcaat gattgcagaa ggactattcg atcctacaca aatcattact cattcgctgc
     6361 cactatcaga agcagcacaa gcatatgaga tctttgacaa aaaagaagat aaaaatataa
     6421 aagttgtttt gaaaccttga attcagtaaa aaatacaggt gattttcttt atgcccaaaa
     6481 tcaaacgaaa tacccatgag atcgaaatag gaaaagaaga agatatcgaa atacgaggac
     6541 gtcgcttccg cttctatttc caaaatcgtt acacattgat ctcactggct gtcgatttat
     6601 taaccgggat tttctatatt tttggaagcc ttgcttcctt aactaaaatc cccgatcgat
     6661 atggcaccta tttttatttg gctggtgcta ttttcttgac gattcgtcct attctcagaa
     6721 tcgtaaggaa catttttatt tacgacgaaa aaaaagcaat gaagagaaca agcaaaaaaa
     6781 aagaggcatg agaatttttc aaatctcatg cctctttttt caccctatcg tatcactttg
     6841 atacagacga gcggtcgtcc cactttcttc cttgaccatt gtcaaaaatg tccactcgta
     6901 tcgttcataa aacgtggtat gatctgtaat taagtaaagt ttttcgtacc caagttgatg
     6961 catttctttt ctgatttccg ctaatatccg cttggctatc ccctgcttac gcttttccgg
     7021 ttcaacaaac agggcacaaa gatttggtgt cagatcaggg cggttatgga agtcgttttc
     7081 aatgacgccc gctcctccga cgatggtttc tttttcatct aagaccacat accattgagg
     7141 aatactttcc ttattagcga ttccagtacg aatactttcc tcatacacaa cagcaggaac
     7201 tccccatttt gatgaaaacc aattcgcgct ttgttttact aattctgggt gttctcttaa
     7261 tttgatccag ttatatgtaa ccaatgttta aacctccgac taaaataaaa cgtttacaaa
     7321 aattatacaa gtctttatga aaattagcaa tgacaacaat agtgatgtaa aaaaaaaaac
     7381 ggaaaacaat cacttgttct ccgttttttg attaatcaag catctaaagc ttcttttctt
     7441 ctttcttgga tcgtagctac cttgacgaac ggtagataga tcaaaataga aagaaccaaa
     7501 ttgacagcgg ctagtacacc acctgcaata ctttttgttg ccaatacacc gccaataatt
     7561 ggtggagtta cccatggagg cataacggta gctgctggca ctaaacctgt agaagtcgca
     7621 aagtaagcaa cactaactaa gaccattggt gtcaaaataa atggaatgaa catgatcggg
     7681 ttcaacacga taggtaaccc aaacatcgtt ggttcattaa tattgaagac acctggcgca
     7741 atcgacaagt ttgttacaac catatatggt ttgtttttac gtcctactaa ataaatagct
     7801 aacagtaaac ctaaagttgc acctgttcct cctaagttga caaagctgtc aaagaaaggt
     7861 ttgttcacga taaacggtgc gctatgtcca gctgcgattg cattaacgtt tgcttcaatg
     7921 gctggcgcat tgattgtttg catcaaagga tcgaccatat tcgctccgtg taaaccaaag
     7981 aaccataaga atggtgtgat aaatgcaagc agtaaggcag atggataaga gtttgcaagt
     8041 cccataaatg gttcttggac cactgtatag aaagaaccta cgatatcatc aacaccgaat
     8101 gcagcaaaaa ttgcagcaac taaagcaaac gcagagatag tgatcattgc tggtagtaat
     8161 gaagcaaatg cttttgcaac tgccggtggc acaccatcag gcattttgat gatcaatttt
     8221 tcatttccgc ttaaacgtac aaagatttct gttgaaataa tcccaacaat aatagcaata
     8281 aacaaaccgt ttgagctcat acctaatgct ccgcctaaag cgaagaaaga gcctaaagaa
     8341 atcacacctg cggaaacacc gtcttttcgg tagccgttag ctaaatgata agcaagcaag
     8401 aaagcaatca agacagaaag aatggcaaat gtaccgttcc aagcagcagc cccgaatcct
     8461 ttccatgatt caccaccaaa aattgagttc atcaattctt ggaacccggg aatcggtaag
     8521 ttattgatca ttactgctaa tgctcctaag atcattaacg gcatactcac cataaatgag
     8581 tcccgaatcg caactaaatg ccgttgtgca ccaatttttg atgctacagg tataaaatgt
     8641 ttctccataa atgcaacaaa tttatccata aatcttccct cttttcttta ctgtagatta
     8701 cgcgttcaat cgacgataca gatcaatgat ctcttttgct aaatctgtaa aagctataga
     8761 tgtcatcaga tgatcttgac tgtggaccgt caaaagcgtg acttcaacgt gatttccttg
     8821 tgcttcttgt gtcagcatct ctgtttgaga gtgatgtgct tggactaaag attcctctgc
     8881 atctttgatt ttttcatctg ctaaagtgaa atcacctttt ttcgcagcag caatcgcttc
     8941 cattgcatcg ctttttgcat ttccgccaaa catgatcaga cccatgatgg cttctagatt
     9001 tttttgatct tccatttatt gtatcctccg ataattttta ttatttgttt attagttttt
     9061 ctgcttggtc gagtactttt tcaccattca tcatgccata gtcagccata ttgattacat
     9121 ctaaaggaat gcctttaggt gctagctttt cagcaaattg attttgcata aagcgtactt
     9181 gcggtcctaa aagtaaaacg tctacatttt tagattctag ttgattatcc gcatctgaag
     9241 cagatacagc aaagatgtct gcttccattc cttttgcttc tgctgccttt tgcatttttg
     9301 tgactaggag acttgtactc attccagctg aacatacaag catgattgtt ttcttttcca
     9361 ttttaaacac gttcctttct atacttacac ttgtatataa gcaagtagcg tgccaacttt
     9421 aaaagcgctt taaaaagttg atatcatcac atttaaggtc tataccaagt tgcatacaca
     9481 ctaaaaaaga aagcgcacac taaacacaca aattaatttt tattaccttt tttcttttaa
     9541 cgtgcacaaa aaaagttgtg acacaaaact tgtcacaact tttttcaaat aaacggtatt
     9601 caaaagcttg catctgcggt aataagtcca accaagttaa aaacataaga tgttgttttt
     9661 actttgctat tcttattact tgatgcagga cagctttttc acaacctctc aataaacggt
     9721 gttcaatcag ctgaccctgg gtattagctc aaaacgctgg attcacaacc tcttttattt
     9781 caaataacca gtaaatacaa tgaacatcta caaaaaactt ctatttattt atagatttcg
     9841 tcaggcaaat ccaattctct cttatcaatc aaagtctgat accacgaagc agattttttc
     9901 aatgttctgc ttcgttcatc gttcaattcg attcgaacta atccatatcg attcttaaat
     9961 gcattcattg gtgacacaca gtcagtgaat gcccataaca tatagccttc gcaatttgcg
    10021 ccttcttctg ttgctttgat caagtaatgc aaatgctgtc cgataaattc gatacggtaa
    10081 tcatcttgca cgattccttt ctcatccata tactgttctt catattctct tcccataccg
    10141 ttttctgtta tcagccaagg gatattatga taattttctt ttagatacat ggctatatca
    10201 tacatgattt tcggatagat ttcccatccc ctggaattat tcattttctt cccaggtaaa
    10261 tcaaagactt cataatatcg ttcaggatgg aaaggtgtct cttcattcca ttgataacga
    10321 ggggcgcgaa cgcgtttagg ataatattga ttgattccaa ggaaatctac tgtatgatgc
    10381 tgaatcgttt ctaaatcctc tgttgcagga tcgaaataaa tcgattgttt ttgacacaac
    10441 tccaggaact cttctgtgta gtatcctttt accatcggat caaaaaatac tcgattataa
    10501 aacaaatcat acatataagc agctttttgg tcttctggag aattggatcg agcatagacc
    10561 atttcaggat ttaaaataca gccaatcctt ccaccataat ttccgctgcg gtaaatctct
    10621 actgctttag ccgttgctaa aactttatga taattccaca tcatccactt ttttgtgttt
    10681 tgttcatgcg gccagcgaac agcatccaaa tagcaacgcg tttggggaac gattggttcg
    10741 ttgaatgtaa accagttttt taccctatca ccgtaacgat caaaggcaat ttgagcatac
    10801 ttagcataaa aatcaactac ttttcttgaa gaccagccat catatttttc caaaagaaaa
    10861 gctgggactt cataatgttc caaacaaatc atcggttcta cgtctgcttc gatcaaagag
    10921 tcgatcattt cacttatatg ctgagcatat tcttcatcta ctatacagtt ctcataatca
    10981 gtaaaaaaac gagcccaatt gatagaagtc cgataatgtg tcagatggat ggatttcatc
    11041 agatccacat cctgctggta ttgttccata aaattagttg ctacggctgg gccataccca
    11101 ttatgccaaa caaaatgttc atttttatac cagctatcta taaacgaatc ctgtccttct
    11161 ttttttcctt tccaaccttc cgtttgccaa gcagaagatg cagcaccaag aataaaattt
    11221 tttggaatag taattttcat gatttcctcc tgttttttag atcccatcat tttccacacg
    11281 atttgctact tttacaaatg gcagatagat caaaatagaa acaataatac aaacaagttg
    11341 cgtaataact gcaccgatat ttcctgctgt tgccagataa gcattgacga tcggcggcat
    11401 tggccaaggg accattacaa ctgctttcgc cgcaaatcct aaagcggttg caaaataacc
    11461 aatcgttcca gtgataagag gcgttaagat aaacggaata gctaaaattg gattcagcat
    11521 gatcggcata ccaaaaataa ctggttcatt gatgttgaag atcccaggag caagggataa
    11581 cttggtgatt tgtttcatat cttcccgttt tccaactaac aatactgcaa tcagtaaacc
    11641 gatcgtgact ccagacccgc ccatactcat atacatatcc caaaatggca tagtaataat
    11701 gttcggtact tcttttccag cttcaaaagc ttccgtattt acagcaattg ccgctaaaag
    11761 caacggttcc cgtatcggct tcaccatttg atttccatgg ataccaatca cccaaaagac
    11821 ttgcgcaata aacatcaata gcaaaatacc cggtaaccct tgtacgacac cttctaatgg
    11881 gcgttgtacg acattgtaaa tgatatcata aaggtacatt cctgtaagtt ctttgatgat
    11941 aaatcccgca gccgcgatac ccgatacagt cagaatcgtc gggaataaag cagaaaaact
    12001 agcagaaaca ttcgttggca cggtatcagg cattctcagc tgtagtttct tctgtttcct
    12061 caaccagcaa taaagttcaa tagaaaggat ggagaccaac attcccaaaa ataacccttt
    12121 cgtatctgta tattgtttag ccaatacgtt ttctactata acagtctgat tttcattcac
    12181 taattcataa acagttggat taacagataa ataagacatc actgcgatga taccagggaa
    12241 tacaccatcc actttattca gcttggctaa ttcgatccct atcagaaaaa cagcatagat
    12301 ggtcaagaaa cttagtgtga catagctgat ggcagatgcg ataggcttta attcttctaa
    12361 aaaacgcaag aaacttatct gtgctaagcc attttccgag tcaaagacca ttgctgaaaa
    12421 caatgtgcca aaagcacccg taataattac cggaatcaat gcagcaaatg cgttttttat
    12481 caccatgata taccttaaat tattgattct cgttgccacg ttccctaaaa cattggctaa
    12541 tttatccata aaactcattc cagatcccct cattcctgct tcttctcctt gacgtcatct
    12601 ctttttgtct attagtcagc gcttacattt cgatgaacag aaactattta ctcctctttt
    12661 ttaccaaata taaaacaaaa attcatctga tgtatttatc ataaaataca tcagatgaac
    12721 tgtcaattag tttctttttc tcctattatt ttctgtaatt ctctccgatt tattgccata
    12781 tggacagtca ttgcatcaaa cgcagcaagt gcattccgcc tttcgatggc ctgaacgatc
    12841 tctgtatgaa tatcgatcgt ctcgattttc agatgctgga tatcataatt tttattaaac
    12901 aaagcaattg attcattaat gattgggata atatgcagca aggctacgtt tccgctagct
    12961 tctgcaatca tcgtatgaaa atcgatgtct aattgaacat gttcttgtgt gccttctcga
    13021 aaagaagttt caatcttttg cgttaatttt ttcatttgga cgatttgatt cttcgttgca
    13081 tattccgctg ccaaacctgc catcttaggc tccaataaat atctcagttc aaataagtct
    13141 cgtatcatcc gatcctgatc ttcaatcaaa gaaaaaccga gtgggtcatt ggcaatccct
    13201 cgcttatcac tgacaaatgt ccccgctcct tgccgaactt caaggatatt cctggaagct
    13261 agagcccgca cagcttctcg caccgtgctt cgccccacct ctaaataatc ggacaattca
    13321 tattcattcg gcagtttatc tcctacttgc aactgcattt cctttaaata tctaaggatc
    13381 ttgtcagtag ttacttctac tagggtttta ttttcttcca aaccacatac ctccttttca
    13441 tctgatgtat tttttcgtaa ttattataac acgtttgaat catctgatga ttttttgtat
    13501 acaaaattga ttaatccgtt catagacata caaacgaaga agatatttac cataaaaaag
    13561 actaggataa aattcactcc tagccttcga tcatttttat tttgaacgac aaaaaatatg
    13621 atgttcaata acagacgatc ttcattcgat tcttgatgtc atcttttgtt ttttttaata
    13681 tatggataaa aagtttgatt acatcgtttc tattctagaa tttggtcgat tctcaattct
    13741 tcttctggtg agaaatctaa attatccatc attcgaacac tttcttgtaa ctgacttaac
    13801 cgacttgctc cgaccagtac acttgtaaca gatttttgtc gcaaattcca tgcaagggcc
    13861 atttgcgcta atgattgtcc tcgtgatacg gcgattgttt gcagtgcttt gattttttcc
    13921 agagttgatc caacttgttc ttctgaaagg aatggtattt ccttacgatg tgctctagaa
    13981 tcttcaggaa taccatgtag ataacgattt gtcagcaacc cttgagcaag cggactgaac
    14041 gtgattgccc ctagcccttc ttcttctaat actggagata gatcgtcttc tagtagtgca
    14101 cgagaaaaca tattgtagcg catctgatga ataatgaacg gtgcttcctt tctttttaaa
    14161 atttcagtca ttttttttgt atcttctcca ttatagttgg atataccaat atataatgct
    14221 tttccttgtc ttacaagttg catcaaagct tctgctgttt cttccaaagg tgtatcaggg
    14281 tctgggcgat gatgatagaa gatgtcaaca taatctagcc ccattctttg gagactttgg
    14341 tcacaacttg aaatgatgga tttacgagag ccccattccc catatggccc ttcccacata
    14401 tgataacctg ctttgctaga aataatcagt tcatcacggt aggcttgaaa atcttctcta
    14461 aaaatacgcc caaagttttt ctctgcagct ccagcagggc ctccataatt attcgcaagg
    14521 tcgaaatggg taattcccat gtcgaatgcg cctcttaaaa tctctctttg attatggata
    14581 ggatcataat caccaaaatt ttgccataat ccgagagata aaatcggtaa tttcaaacct
    14641 gaatgaccgg atttacgata ttccattttt tcataacgtg cactatctgc aatatacaat
    14701 ttttctcctc ctcatcaaat ctttttaaaa cgctttcttt tttctatttt tagtttacta
    14761 cattttaaag aaaaggagtt aatcaaaatg attttttcgt atattttttt gccatttgtt
    14821 ttttactcgt tgatagatca gaaattgatt acgtgcttgc tggatttttt tataacgttt
    14881 cggttgtttt ttgtaaaatt gctgatgata gttttctgct ggccaaaacg taccggctgg
    14941 ttcgattttt gtgacgattg gttggtggaa agttcctgaa ctgtctagtt tctgttttga
    15001 ttgttccgct aattcttttt gtcgttcgac ttgataaaaa atcactggac gatattgctt
    15061 ccctctatct tgaaattgtc caaatgtatc tgtgggatca gtaatttgcc aatagattgt
    15121 cagcaactcg ctataactta tttctcttgt gtcataaatg atctccacag cttccacatg
    15181 acctgtgtaa ccaccactta cttgatcata atttggtttt tcaacatgtc cgcccgtgta
    15241 tcctgacaaa acagatagga tccctttttt cgattcaaac ggctcgacca tacaccagaa
    15301 acatccccct gcaaatactg cacgttcttg atagtcagca tcttgcatgg catgcttgga
    15361 aaagtcatat ttttgctcct tatcaaatcc ttccgtaatc aataaataaa agtccattac
    15421 atcaggcgtc agattcattc ggatagctaa tggacgtagt gttgcttcta gttttgataa
    15481 ctgttcttta acggatatat tttctcttat gtcattcttt gtctgaatca atacttttcg
    15541 ttcccaatct cgtgtattcg gattcaaaat cagattataa agtgtttgaa tgaatatttg
    15601 ttcctcatcc atagattctc ctcctttccg aagaagacaa gctagtgact ttagctgtaa
    15661 tttatcataa agtgaggaaa taagcgatta cttcatcccc ctacagattt ttcgtataaa
    15721 aaagagagaa tccttatcga cagattctcc tttttaatca atttatttta taaatgaaaa
    15781 gttaatttac cttttatgga aaactatttt aaaaaataaa cataaatatg cagttaatgt
    15841 aacaagataa gaaataatag tcgttactgt ataacttgtg cttactttaa tatcagcaac
    15901 tcctttgact ccgcttattt gtaccaatcc attagcattc acctgctcct ttacaggttg
    15961 atgatttatt tctacattcg ttcctaaata agcatataca ggaagtgtga ttacttggct
    16021 cttctcatta gttactctat atatgataga actaccagtt gttttataag gaaggtttac
    16081 ccattgactg ccaatctttg cttcgtgttt tattatatgc tccttattct ccaatgaagc
    16141 gataggagaa taatcatata aaaaataatc cattccttta gtggtatacg cctctactgt
    16201 ttctttgttt acaacaatat gtcccggctg agaaattatt ttttgattga aatttttttt
    16261 actagaaaca gttaaagtga ttaataacaa aacagtcata aataaataaa atagttctct
    16321 tgtcttattg tatcttctat ttttgaaaaa tatcatcgtt aatatcagag aagagaatat
    16381 tatttgcaag cttaagatcc gccaaggaaa ttgtaacaat ttggctggcg tattttgcag
    16441 aagataccat ggcaccaaac tagttgtagc tagccaagtc aaacacgtac cataataaat
    16501 ctttttttct atgtttgt
//
LOCUS       DACECZ010000054        16493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.054, whole genome shotgun sequence.
ACCESSION   DACECZ010000054 DACECZ010000000
VERSION     DACECZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.054"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(106..381)
                     /locus_tag="IWO35_001601"
     CDS             complement(106..381)
                     /locus_tag="IWO35_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ0316662.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(624..1934)
                     /locus_tag="IWO35_001602"
     CDS             complement(624..1934)
                     /locus_tag="IWO35_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ0316663.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2116..3345)
                     /gene="rpsA"
                     /locus_tag="IWO35_001603"
     CDS             complement(2116..3345)
                     /gene="rpsA"
                     /locus_tag="IWO35_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ0316664.1"
                     /translation="MTEFDQNQENNQSMEDGMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3480..4160)
                     /locus_tag="IWO35_001604"
     CDS             complement(3480..4160)
                     /locus_tag="IWO35_001604"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ0316665.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4209..4844)
                     /locus_tag="IWO35_001605"
     CDS             complement(4209..4844)
                     /locus_tag="IWO35_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0316666.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4910..6313)
                     /locus_tag="IWO35_001606"
     CDS             complement(4910..6313)
                     /locus_tag="IWO35_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ0316667.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6300..7331)
                     /locus_tag="IWO35_001607"
     CDS             complement(6300..7331)
                     /locus_tag="IWO35_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316668.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7362..7583
                     /locus_tag="IWO35_001608"
     CDS             7362..7583
                     /locus_tag="IWO35_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ0316669.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7748..8485)
                     /locus_tag="IWO35_001609"
     CDS             complement(7748..8485)
                     /locus_tag="IWO35_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ0316670.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8638..9978)
                     /locus_tag="IWO35_001610"
     CDS             complement(8638..9978)
                     /locus_tag="IWO35_001610"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ0316671.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10215..11738)
                     /locus_tag="IWO35_001611"
     CDS             complement(10215..11738)
                     /locus_tag="IWO35_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ0316672.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12116..12727)
                     /locus_tag="IWO35_001612"
     CDS             complement(12116..12727)
                     /locus_tag="IWO35_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0316673.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12850..12975)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13057..13773)
                     /locus_tag="IWO35_001613"
     CDS             complement(13057..13773)
                     /locus_tag="IWO35_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0316674.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13779..14348)
                     /gene="scpB"
                     /locus_tag="IWO35_001614"
     CDS             complement(13779..14348)
                     /gene="scpB"
                     /locus_tag="IWO35_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ0316675.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14332..15129)
                     /locus_tag="IWO35_001615"
     CDS             complement(14332..15129)
                     /locus_tag="IWO35_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ0316676.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15099..15470)
                     /locus_tag="IWO35_001616"
     CDS             complement(15099..15470)
                     /locus_tag="IWO35_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0316677.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15489..16376)
                     /gene="xerD"
                     /locus_tag="IWO35_001617"
     CDS             complement(15489..16376)
                     /gene="xerD"
                     /locus_tag="IWO35_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ0316678.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4630 a         3575 c         2793 g         5495 t
ORIGIN      
        1 gacatacgga ttcggaatct ctgtctctta tttattcctt ttctgctagg aaataaaaag
       61 aaaagcactt gtagccctag agccacaagc gctttttcaa ctgtcttatt taacagcgtc
      121 ttttaatgct ttacctggtt tgaatgcagg tactttgcta gctggaattt cgatttcttc
      181 accagtttgt gggttacgac ctttgcgagc tgcgcgttcg cgaacttcaa agttaccaaa
      241 accgattaat tgaacttttt cgccgttagc taaagcatct tgaatagttg aaaatacagc
      301 atctactgct gcagttgcat cttttttagt taaatcagta gctgaagcaa ctttttcgat
      361 taattctgct ttgtttgcca tggatagatt cacctcctcc acaagagata tgatagaaat
      421 atcatttagt taaaactttc tgagtggtcc agaagtttaa aaagaaatat tgattaactt
      481 caatattctg ttatcacgat atcatatgaa ccgcttaata gcaaggtttt taccaatttt
      541 cacatgcttt ttttagtcat agaaacgtaa caaacgaaac tgtctttttc ttttgaaaaa
      601 aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag ggataattcg gatggacgtc
      661 ccttcaaaag taaatgcttt tctgatttga ttttctaaga aacgttcata agaaaaatgc
      721 atcaattcct cttcattgac aaaaacgaca aatgtaggtg gtttgacagc gacttgtgta
      781 gcataaaaaa tcttcaggcg ttttccttta tctgttggtg ttggattgat tgctacagca
      841 tccataatga tatcattcaa cacagccgaa gagatacgaa gattctggtt catacttact
      901 tgttcgatca tttcaggcag acgttccaac cgttgtttag tcactgcaga aacaaaaacg
      961 attggagcgt aatcaagata ccggaattca tcacgaatct cttgttcgaa atcacgcatt
     1021 gtattggttt ctttctttac aagatcccat ttattcacga cgataataat tccacgacca
     1081 gcttcatggg cgtaacccgc tactcgcttg tcttgttccc gaatcccttc ttcagcattc
     1141 agtaccatca aaacgatatc agaacggtca atggcacgca ttgcacgcat cacgctgtat
     1201 ttttcagtat tttcgtatac ttttccgcgt ttacgcatac cagctgtatc tatcattaag
     1261 aatttctgac cactttcaga aacgaagtgg gtgtcgattg cgtctcgggt cgttccttca
     1321 atatccgaaa caattacacg ttcttctcct aaaatcgcat tgatcaaaga ggatttacca
     1381 acatttgggc gaccaatcaa actaaactta atcgtgtcgt cgtcctcttc ttcagtctct
     1441 tcagagaaat acttcactgc ttcatctaaa atatcaccaa tccctagtcc atggcttcct
     1501 gaaacaggat aaggatcgcc tagccctaag gaataaaatt catagatatc gtttctcatt
     1561 tctggattat ctactttatt gactgctaaa atcactggtt tattacttct gtataaaatt
     1621 ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc cgctgactac acaaatgatt
     1681 acatctgctt cttcaatcgc gatttctgct tgatgcttga tctgatccat aaaaggttca
     1741 tcgccaagat caattccgcc tgtgtcgatc acgctgaatt ctcgacctaa ccattcacct
     1801 tttgcataaa tacgatcacg agttactcca ggagtatctt cgacaatcga gatacgctca
     1861 ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg ggcggccaac gattgcaatt
     1921 gttggatttg ccatattatt tcctcctcac aagtttccaa tctcacttag tttaacgaat
     1981 gcattgccaa ctttcaagca tagattgtca attaaacgaa gtatttttat tccttattga
     2041 aatactaatc gcttttcaaa agaaaaactg agacaaaagc agctgctttc atctcagtca
     2101 cagatacaaa atcaattatt tttcagaatc atcggttgct ttttcttcta cttcttcttt
     2161 caacgcatca cctaaaatgt ctcccatagt aaatccagta ttttcttctg gtaattcata
     2221 agactcaaca ttttttggtt cttcttctga agctggtttt tcttctaaag ctttgatact
     2281 caatgcaata cgatgttctt ctggatggat ctctaatact ttcactttca cttcgtcacc
     2341 ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt gaaatttgag agatatggac
     2401 tagcccttct actccaggga acacttcaac aaaggcaccg aagcttgtca aacgtttaac
     2461 tgttccgtct aatactgtgc caactggagc ttttgtatcg atttctgacc aaggtccagg
     2521 ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca ggattgatag acaacacttt
     2581 gacatttaca tcatcattga cagccaatac atcacttggt tttgctacat gactatgtga
     2641 aatctctgag acatggacta gaccgtcaat tccgcctagg tcgacaaatg cgccaaaatc
     2701 agtaagacgt gcaacacgtc cactgagaac atctccctca tggatagttg aaagaatttc
     2761 ttttttcttt tcttctcttt cgttttccac taccgcttta tgcgataaga tcaaacgatt
     2821 ttctgatggt tcgatttcga tgattttaaa gctcaatgtt ttgcctttat agtctgaaaa
     2881 gtctgcaaca aaatgatctt ccaccattga tgcagggaca aaaccacgta caccgacgtc
     2941 aacgactaaa ccgcctttga caacatttgt aacaggtgct tcgatgacct tgccctcttg
     3001 gaaatctctt tcgatatctt cccaaacttt tttagcatct aaacggcgtt ttgaaagtag
     3061 gtagctgccg ttttctttat ctttgccgat cgtactgata acgactaaat ctaatacatc
     3121 accgactttt actacttcgc tgatatcttc taccggcaaa gttgataatt cttttgccgg
     3181 aacgactcct tcaactccag ccccttcgat tccgacaatc acttgtttgt cttcaatgac
     3241 taaaacttcg cccttaacaa catcgccaac ttttacttct tgtacgcttt tcattccgtc
     3301 ttccattgac tggttgttct cttgattttg atcaaattct gtcataacac ttgtcctcct
     3361 aaccaacatt ctgccgtaag tacggtactg tttttagttt aattgattat tcaaaaaaaa
     3421 taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt tttcattttt tttcttaatt
     3481 taaaaaagaa gataaccttt tttcaaaaca acagctttta ttgctgcaac gacttctgga
     3541 atcgatttgc cagtcgtatc gatacgaaca gcatcctctg cttgcttcag cggagagacg
     3601 tcccttgttg aatcataatg gtctctttcc tgtatctctt tcgtcagtgt ttcaagatca
     3661 gtcggaattc ctttttcttg gttctctttg aaacgtcttt ctgcacgctc acttacgctt
     3721 gctactaaaa agattttcac ttctgcattc ggtaatacag ctgtcccgat gtctcttccg
     3781 tccatgacaa cgccaccgcg ttcccctatt tttcgttgga cagcaactaa ttcttctctg
     3841 acttttccat gtgcagagac ttcagaaaca gcttttgtga catctggttg acggatttct
     3901 aaagtcacat cttttgatgc tatgaataca tgctgtccat cttcttgttg cgaaaaagag
     3961 ataggatact ttttgatcag cgaaactagc ccttcttcat ctgaaaaaga tacattgttc
     4021 gtaatagcta aataagttac tgcgcgatac atggcaccgg tatctgtata aatataattc
     4081 aattcttttg ctaatatctt ggcaacggta cttttcccag atgaagcagg cccgtcgatt
     4141 gcaatactga tttgtgtcat gatataactc ctttttctca gaaaaaaagc cgcaaagcgc
     4201 ggctattatt atttaattct taattcttga ccagggtaaa gcataaagtt gtttggatct
     4261 aagccattca attcaaacaa ttgatcagtg gtgatccctg cacgctcagc cacttgtttt
     4321 ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg cagctgatga agaaggtgtt
     4381 gtttctgctg cattgctgct tgaatcccct gcttgataag ttgcctcact tgaggaagtc
     4441 ggtgttgtct ctgctgcaat actgctttct gacgttacac ctgtatcttg agacacagta
     4501 gattcttccg cgattgtact agattctact gttgaagtct cagttgactc ctcttttgtt
     4561 gtacttgtag aactttctac tactgatgtc gaagtttcag aagaagttgc tgctgttgca
     4621 ttgttgcttg atccatttct gatccagaaa taagcacctg ctggaatagc gatacataag
     4681 accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc ctttcttttg ttgtctctga
     4741 gaacttcttg atgaaacttc atcatctgta tcgtagattg gttgttccca tggttcttgt
     4801 gtttcgttat tttctgtaga ttgatgtcta tcttttctgc tcactgtttt acctcctaaa
     4861 ctctatcgtg tgtattatac cataggaaaa agtgtcttgt cctaaaaaat caatgttcca
     4921 tgtttttttt aaataaattt agtaaaatcg tttcccaatg aagttgttca gtcttagaaa
     4981 aaagcttcct ctttggcaga agttctgctt ttgctccatc aatatcacaa caattctgcg
     5041 gcttttcgga gagtttttcg ccaaaataag ctaagatgaa ttttctgcgg cagttttctt
     5101 cattaatata gcgaagcatc tgctgcaggc ggaattcttt ttctttttcc tgacgtttga
     5161 gccgttccac ccaattttct ggtttttcag actgctggat cagctccatc catttttttt
     5221 gcagctcatc aaagggagct tgctttgtgg catattccaa ataaagctca aaactttgtc
     5281 gctgttctct agaaagttga ttaaagaaat aatggatacg ttcatcccca ctttgataaa
     5341 aaagaattgc tgcactttct tcctgatctc tgcctgctcg accgatttct tgtacatagt
     5401 tttctagact gtctggcaaa tcataatgga ccacataacg gatatcagat ttatcgatcc
     5461 ccataccaaa tgcattcgta gcaatgagaa actgcagctg attttttaca aactgctgtt
     5521 gaagttgtct tctttgagct gcatcaagac caccgtgata gtatccaact gtaaaacgtt
     5581 ccctgaataa atgatacaat cgttcgactt ctttttttgt tgcacaataa atgattgccg
     5641 ctccttgtgc tctttccatg aagtgttcaa gttcttgttc tttttgctgc gttttccgaa
     5701 caaaaagcga gatattttgc cgattgactg attgtctgat ctcttttggt ggtcgatcaa
     5761 atagtacatg acgtatatct cttgctacta aatctgttgc tgtcgccgtt agtgccagtg
     5821 tgaccgggtt tcctaaaagc tttcggattt ttcctaactg ttgatattct ggacggaaat
     5881 caactcccca ttgagacacg caatgagcct catctactac atataacgca atattttgtt
     5941 ttttcagatg ctctaaaaga gaaggattag tcaacatttc gggactcaaa aacaaaaatt
     6001 tatattgcga tagatgacgg aggacgtatc ttctttcaga ttgcgaaagt aaactattga
     6061 aagcagctac tcgtttctcc cctcgttttt gtaattgcgt cacttgatct tccattagag
     6121 aaagcagcgg tgacacgatg atgaccagac cttccattaa ataccccgtc aattgatagc
     6181 aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt atcttctcca tcaagcaacg
     6241 cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa tccaaaatat ttttttaatt
     6301 catttattaa cattctgtat cgcttccttt ctcaatagat agatttgata caaacgaaat
     6361 tctccataat ctaattgtcc atctacattc cattcgctaa agcgccactc tcgcacggaa
     6421 tccttatttg gttccaaacg ttcgattgtt tctgcagaga gaatccactc gtatggaaaa
     6481 tcatcaaaaa acaaggccca ctcaataaga tgatctgtta ctgttcctct ttttaaatga
     6541 cggattgcca tgatctggtc aattgtctga ccattcataa acatctgctt ggtgatcatc
     6601 atgctttgat tgaaattttg ctgtaataat gaatcgatca aagcgtgcca aagcgaatct
     6661 ggccgttttt cgatttgtgc taacaaaagg tggatacatc tgctttgttg cagataaacc
     6721 tctgactcat cattgttttt tgccagctga taaggaagca gccctgttcg ctcatttcca
     6781 gataattggt tagctaaaaa atctgctgct tcttctggta tctccgaaaa cagctgcacc
     6841 aaactctcat acgcagtatc aatcaaaatt tcacgtggca attggctttc actaagccat
     6901 ttttttagtt ggaaagtata aaaaggactt gtctctgctg gaagatagtc ttgaataccc
     6961 gttgccagat tagaaattac ttgaacagca aatttaatga gccgccaact agtttcccct
     7021 gttctgccat agcggtcaaa acgcaaacca gtatgttcta tttgatgttc ggacaacata
     7081 tcagcccctg caggcgttag ctttgcttca ttttcattta tttcgatcca accttgctgc
     7141 gcaatttttt gcaaaatttg ataaaactta tcctgcttca attctggaaa actgttatgg
     7201 ataaacaaaa gttcgtgggt aaatccataa ataagtactg aagtcgttcg ttttcccaca
     7261 aggagttggt aaagagaact aatccgcagc ttgtgtcctg atgaaaatag cgataaaata
     7321 aaaacaatca tattattctc cttaagttag gatggttaga aatgtcacta ctatgtaaac
     7381 ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat cgaagcgcca gatattttcg
     7441 attatgatga agaaggaatc gttttatttg cccaagaacc agaggctcat caacagttcg
     7501 tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca aaaatgtcca gttcgagcga
     7561 ttttattaga aaataaaata taaatataaa cgaaacttta aaaacgtaca ctaagttctc
     7621 taaacttgaa aataagccgg gatctgtttc tcttgttccg tgaaatcccg acttattttg
     7681 aaagattcat ttgctatcta tagttaattg actagttttt tcacggaccc tataggaccc
     7741 atttatatta tttcaacttt ttcagccagt tttccaatgt attgacgatc acagtttgct
     7801 ggtctgcgtt agaaatttct gcctctccat ctcctttttg ctcaccatag ctgccaaaac
     7861 ctccatgatt tccgccattg attgatacat actctgttct ttccggtaaa tactgcttag
     7921 cttcttgata agcttcctga ttcaatactt tatcttttgt cgccgtgatc gataaaacag
     7981 gaacagctga ttctgtcaat gttccttttt tgtcgggata acttgccatg aagaataccc
     8041 cttttaaatg tttgtctgaa tgattcttat tgacataacg acttgccatt acgccaccta
     8101 atgaatggcc accgatcaca taatcggaat ttggaaaatc ttctttgacc ttatccgctt
     8161 tgtttccgcc catcactgct agatcaagtg gcattttgac aatagcgact gaaaagccgt
     8221 tttttgctaa atcttgcgcc caaatactat aactttctgg ctctacgaga gcaccaggat
     8281 aaaaaataag taatggagac tttccatgat tctttttctc tccttcaaaa aataagtaat
     8341 cttctttgtt ttttccttct tttaccaaca cttgcgcttc tgacgatggt tcgtacgtat
     8401 tttgctgtat atagtaataa ccggctccac cgagtaatcc taaaatgatg actaagccaa
     8461 ttagtattcg tctaaatatt ttcactttct cccacctttt ctatctgagt gacccctttt
     8521 tcttaatcaa gtatgattat accttaaaaa aataagagag aaatgcaaac tttccagcat
     8581 caaataaaaa aagacggaat atccatttga atatccgccc tttttgtatg tttattttta
     8641 tttagcacat aaacgataca cagcttctgc ataaatttcc attgctttat acatactact
     8701 taattcccat cgttcattcg cctggtgcat aaagtcagga gtatcaggga acatcgcacc
     8761 gaatgccaca cactggttca tcgtacgagc aaaagtagcg ccgcctgata caaacggttc
     8821 agtcatgtct ccagtaatat cgcgatatgt ccccaataga tttttcacta actcactgtc
     8881 tagtggcacg tataatgaat caaggaagtc aaattcttca taagtcaacc catatttttc
     8941 tgctgtttta gttaatttag ctactaaatc atctttcttg aatgtaacag ggattctcat
     9001 atctacaccg atttttgttt gttctggtgt gatttccaaa cttgcaaagt tcatcgttag
     9061 ctcgccggat tgttcatctt ctgttttgcc cacgacagct tcaccagttg cattttcttg
     9121 aactaatttt cctaagaaat tgattgggcc aaaatcaaat acttctgaaa gagcaattgc
     9181 cagacgagaa atagcattca ctccttgtgc tgcatctttc gcgtggatac ttttaccaag
     9241 aacgacgatg ccttcacctt gttcttcgaa atcgaattcg tgttttttca atgcttcttt
     9301 tacttcagaa agcttttctc cagagtaagg agctgcatca ggaacaacat tcaacgctcc
     9361 tccagctttt acagaaaact cgttcgttcc tggtcctgtc aagtaagctt gcaataagcc
     9421 tttttctgca taaattaaag gaaattcagc atctggcgca aatccttgcg taattccttc
     9481 ttctttttcg ttatattttt ctaaacaacg ccataaattt tcttcatctg taccaaagat
     9541 aaaacgaata cgtgtcttga attccacgcc agcatcaatc aaagctttga cggcatacat
     9601 tgccgcaata gaaggacctt tgtcatcttg ggatccgcga ccaactaacc agccgtcttt
     9661 tatagttgga tcgaatggtt ttgtttccca attattttcg tcccctgctg gaacaacgtc
     9721 catatgacac aagataccga ataattcatc tccagagcca atctctgaat aaccgtaata
     9781 gccttctgga tctttaaatg ttctaaatcc tagcttttca ctgatttcca atactttatc
     9841 caaagcttca atcacttttt taccgaaggg atgtccttgt cctgaatctg cttcatctag
     9901 tacggaagga attcggatca attcgctcaa tgattctaat gcttgttcat gatgttgttc
     9961 tgtcacaaat tttttcatga tattatcctc ctaatatgtt aaaaagggtc atgtcaaatt
    10021 cctccgacac aacctcttct tctcaaataa tgacgtttta agcctgctcc atcagcaagc
    10081 agaatgttct taacaaatga tgaaagaaaa aacactcact gaatggagct attgacttgt
    10141 ccacgtgctt atcgatagcc gtttatcggt ttcttcattc ttatgataga ccttgtacct
    10201 gtatttgtct aggtttagaa cattgctgct acacctagga tcacacagat ggctacaaat
    10261 aatatcgcaa ttaatttacc ggtaaatttc caccacactg aaacatcgaa acgagcaatt
    10321 gctacagcac ccattacgat agcagatgtt ggtgtaatta agtttactaa accagatgca
    10381 gattgataag cagtgattac taaatcctta ccgacgccgg caaattcacc cataggtccc
    10441 atgattccca ttgttgcagc agctaagccg gaagttgaag gaatcaagaa tgacataggg
    10501 atatagaaaa taaatgtaat aatgatgaat acacttgaag ataatgaact taatccttgt
    10561 tcgccccaat gtaagatggt gtctgtgatt aatccattgt tcataaccac ttggatacca
    10621 cgagcaacag ctactacgat tgctacaccg atcaaatcag aagcaccagc taaaaattca
    10681 gagacaaatt cagactcttt catgccataa gtgattgcta ctacgatgga cattactaag
    10741 aataacatag taatttctgg gaagtaccag tcacctaatg gaagcatgct tttacctaga
    10801 accgtaccta gtaccggaag gtttgttaac cagtttgtca atgattcaaa aatcgtgaaa
    10861 ttcgcattca aggttgtcca aggaatcaaa ctgatgatca tgataccaaa agtaataaag
    10921 aacaatacat taacatgttt ttgttttttc gtgattactt cttgacgacc gattgcttca
    10981 atatcgaaat gcttcatatc ttctttacgt tggttgtata ctaatgattt tgttggatct
    11041 ttttcgatct ttgatgcata acgatacaca tatacaatag aaatagctaa caaaataacg
    11101 aacatcaaga atcgaagacc aataccttca cccatcgaaa tcccaacagc ttgtgaagct
    11161 acgcctgttg caaaaggatt taccgttgaa gctagacagc ctacttgaga ccctactaat
    11221 acgattgcta cagctactac tgtatcaaag cctacagcca tcatgactgg gatcagtaaa
    11281 gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag agccgcctaa tgcaaataac
    11341 agcatcaata ctggaataag cattttttct tttcctttga atttcttcac aactgaagca
    11401 atcccggcat ctagtgcccc tgttttgttg ataataccta aaaatccacc aacaaccaaa
    11461 atgaagaaag agattgggat tgcagcaggt gtttctttag ttagctcccc cgctggtaca
    11521 cctagcatcc cattgattgg cgccatgaaa atatcccaca gcccttgagg attttgttgt
    11581 actcgttcat atgatccagc gacgatgttg cctgcatcgt ctacactata gtgcccagct
    11641 ggtataaacc aagtaagtac agcgattact gcaataataa taaataagac cgtatacgct
    11701 gaaggcattt gaaatttttt cttttttgtt tgttccatct tcttcttcct ttcttttccg
    11761 ttagagacaa caattttggc tctaatgtta ttttatcaaa aagagtatca gaacaaaaat
    11821 aataacacgc attttgtaaa acttttgttc aaaaagttcg tttttcccta ttccctcctc
    11881 ttttttcagt tttttaaagc tttcttcttt ttcattgtag cgtttccttt tttttataaa
    11941 caaacatttt cgtcttttaa aaggagaaga atttcacatt tgcaaagaat tgattgattt
    12001 ttatgcataa atcaaaaaaa tactcaaaac aaacaaataa aacgtttaca ttattgcatg
    12061 tcttgtattt ttttccaaaa aaaaagacca agaaatttct tgatcttttt gatatttatt
    12121 tgactgctgt atgacgttgt tcgaactgat gttgttttcg acttaaccat ggaagaagtt
    12181 gcgcatgcag cactaaaaag acagcactca cgatgaatcc tttgatcaag ttgaaaggaa
    12241 cgatcccaat cagtacatag tttgctaaag acatgcccag catctgtgtt gcgttcagcc
    12301 ctaaaaacat taagtaaaga ggcgtaataa cgaaataatt tgcgatactc ataaaaatag
    12361 ttagagcgat ggttcctgta atgacaccta aaaatttgtt tcttccttta tgctgtttct
    12421 ttacactaaa gaaataataa atcgggaatg taaagatcgt tgttgataag aaactagcag
    12481 tgtctcctac caaattatcc ggagaaaaac ctgtagtcag cagatgtaat actgaacgca
    12541 agaacgctgt tgcaactccg gccgctggac cgaataagaa catgctgatc aataccgaga
    12601 tatcactaaa atcgatcttc aaaaaactaa aagccggcat gatcggaaaa gcaaaatact
    12661 gtaaaactaa tgcgatcgca gcaaacatcg ctactgcaac cattttctgt acacgggtgt
    12721 tcttcatttg aaatcctcct gttaggactc ctcttcagcg aactttttga cgtttctctc
    12781 caaatgaaaa aactctattt tccagggaaa atagagttag agttatttga tcaagcgtcg
    12841 tcaaataagc tctatcttct ttatccagac tatactgtcg gtatcggaat ttcaccgatt
    12901 cagctatttt tttgcaaaat aggtcgtgga ctataaccac cggtcgggaa tttcaccctg
    12961 cccctgaaga cgaaccttat ataatttttg tttacatatt cattatacat atctattcaa
    13021 aaaacgcaac atctaacaat tataaaaagt aagtgttcat tttgagtggt tcagcaaact
    13081 gctgacttct tttttgttca gttcacggta atccccagga cgtaggcctt gtaaagtcag
    13141 atctccgtat ttttcacgtt ttagtttttg cacaggaaga ccaactgctt gaagcatatt
    13201 tttcacttga tgattacgtc cttcgtggat cgtcaattcc acgatactgt ggtttgtctt
    13261 aggatccact gataaaattt ggaatttagc tggagatgtt tttcgtcctt cgatctttat
    13321 tccttttgtc aaaggtgcaa gcatcgattt tgtagcgatc ccttttacct ttgcaacgta
    13381 aactttatcc acttcatgtt ttggatgtgt caggcgctga gcaaaatctc catcatttgt
    13441 cagtagcaag atccccgaag tatcgtaatc cagtcgacct acaggataga tacgttcttt
    13501 taccatcggc aaaagatccg taacaacttt tcttcctttg tcatctgcaa cagcagaaat
    13561 aactccttta ggtttgtaca ataaatagta accgtattct tcttgataaa ttgggacttc
    13621 atccacttcc acagtgtcat gtttcccaac ttttatccct aattccctta caacttgtcc
    13681 gtttactttt acacgtcctg caaggatgaa ttcttctgct tttcgacgag aagtgatgcc
    13741 agcgtgggca attactttct gcaatctctc cattacaatt attctccttt attcattcca
    13801 tcaaagaata gatctggtga cacgtcttct tcaagtgaaa tttccatttc ttgtgtatca
    13861 ggcagttctt ctaatgattt caaaccaaaa taatctaaga agtagccagt tgttccatat
    13921 aatatcggcc gacctggtcc ttctactcta cctttttctt caatcagttt atgggcagct
    13981 agacgctgta ccgctcctga aacctgaact cctcgaatct gctcgatttc agctcttgtg
    14041 attggctgtt tataagcgat gattgctaag gtttcgacgg cggcttgaga taatgtgttt
    14101 gatgtccctt gcgcatatcg cttaagtaag ggagccagct cttttttagt cgttaataca
    14161 taatggcttt ccgtttcaaa aatatgcaaa gctgagtgcg agttttgagt gtaagattct
    14221 tttaattctt tcactaaact tgtgacttct gcttcgctga gatttaacag tccgcttaat
    14281 tcttcattac tgatcccttc ctcgccaaca ataaaaagaa gggcttcaag ttcacttaat
    14341 ttcgtcatgt tcggtatctc ctactgctgg ataaagcata attgattcat agttttcttt
    14401 ttgttgcgca atgatctgcc cttttttcat caattctagc aaagccataa aagtagtgac
    14461 aacttcactt ttagaaggtg actcaaaaaa agaatcgaaa gtacagccat gcgcatttct
    14521 ttttatacgc tcttcgataa ataccattcg ttcttcgatc gttgtgtcat cgggcgtgat
    14581 cgttttttcc actttttgag aaaatttctt acgttctaag attttctgca tggcacggat
    14641 caaatcgatt tttttgactt gtcctcgctt caacggcttt tcagcatccg ctaacccatc
    14701 gatgttcata ggttcttttg tgtaataaag acttcgttcc tctgcttttt ctgtcagtct
    14761 ttcagctgca tatttatatt tacggtattc taaaagctga gtgaccaatt cttctcgagg
    14821 atcttcttcc cataattctt catcgtctgt gccgaattct tggacaggaa gcaatgtctt
    14881 gcttttgatt gccatcaatg tagcagccat cactaagtat tctcctgcaa cagctagctc
    14941 gagtgttttc attgtatgga tataattcat atattgctcc gtgatcgtag cgatcggtat
    15001 atcatatata tcgatttcca tttgctgaat cagatgaagc agcagatcga gaggaccctc
    15061 gaatacatct aatttaatat tgatctccgt caactcattc actctttctt ctttttcttt
    15121 gctagccatt ttccaatgta aggctctgtg atttcagttg cacggatgga gacatccggg
    15181 tacttttcct gtaattcatc taacatttga aagcccatat tctcaccgcg ataagacgga
    15241 ttcaaagaaa tgtcatggat gatcagctga tcgttcgttg cccagtaaat accaataatc
    15301 ccttgaatgt tggttgaacc ttctggcgta aagaaaaaaa gttcataatc gtcttcagat
    15361 tcgtacgtat caatctcctt caacaaagaa gttttacctg tcagtttctt atggaaactc
    15421 aatagtccca tagcaatttt acggttttct ttccggtaat gtgttaacat cttctcacct
    15481 tcctatgact aagcccttgg aaaatgctgc ttataaacat ccgtcattct tttcttcgtg
    15541 atatgtgtat aaatctgagt cgtagagata tccgcgtgac ctaacaattc ctgtactgtc
    15601 cgcaaatcag ctccattttc tagcagatgt gtggcaaagc tatgccgcag cgtatgtggt
    15661 gttacttctt tgtaaatccc agcctctcgt actaactgct tcagattttt ccagattcct
    15721 tgccgagaaa gacctttccc atgattattg acaaatacat gcatttcaga tgcgtccttt
    15781 acaagtaatg gacgtgcttc gtctaagtag cgttccagcc attggatcgc ataatcacct
    15841 aaaggaatga tccgctcttt atctcctttt ccaactgtct gtaccagtcc aagagaaaga
    15901 tgaagatctc ccaatttcaa tccaatcaat tcactgacgc gcatcccagt agcatacatg
    15961 acttccaaga ttgcacgatc tcttatccct aacgtttttg tcgtatcagg tgcttcaatc
    16021 agcttttcta cttctgtcag agtcaatgta cttggtaatt tctgtgcttt cttcggcgta
    16081 tcgatatgtt gcatcggatc atgatcagtg atacgctctt gccgtaaaaa ctgatggaat
    16141 cttctgagac ttgagatcat ccgagtaaca gttgctgtcg cttttttttc ttcatgcaac
    16201 ttctccagaa aacggatgac taaaaagcga tcgacatcct gccatgaagc aattttctgc
    16261 tcctgtagaa aatgcaagta ctgttccaaa tcacgttcat agctttgtcg tgtattggcg
    16321 gacaatcccc gttcaatatt caagtagtgg agataatcgt tgatttgctc tttcataata
    16381 atccccctca tgcctctcta tcatatcaga taccgcttca ttctgatagt ctttttccat
    16441 aatctgagca aagaaaaagc cgtctagaca ctcagacgga ctggtttctt ttt
//
LOCUS       DACECZ010000055        16479 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.055, whole genome shotgun sequence.
ACCESSION   DACECZ010000055 DACECZ010000000
VERSION     DACECZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.055"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(15..212)
                     /locus_tag="IWO35_001618"
     CDS             complement(15..212)
                     /locus_tag="IWO35_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ0316679.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(606..2333)
                     /locus_tag="IWO35_001619"
     CDS             complement(606..2333)
                     /locus_tag="IWO35_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0316680.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2326..3519)
                     /locus_tag="IWO35_001620"
     CDS             complement(2326..3519)
                     /locus_tag="IWO35_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0316681.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3706..4350)
                     /locus_tag="IWO35_001621"
     CDS             complement(3706..4350)
                     /locus_tag="IWO35_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0316682.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4444..4578)
                     /locus_tag="IWO35_001622"
     CDS             complement(4444..4578)
                     /locus_tag="IWO35_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ0316683.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4580..6712)
                     /gene="feoB"
                     /locus_tag="IWO35_001623"
     CDS             complement(4580..6712)
                     /gene="feoB"
                     /locus_tag="IWO35_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ0316684.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6709..7182)
                     /locus_tag="IWO35_001624"
     CDS             complement(6709..7182)
                     /locus_tag="IWO35_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ0316685.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7583..7807
                     /gene="nrdH"
                     /locus_tag="IWO35_001625"
     CDS             7583..7807
                     /gene="nrdH"
                     /locus_tag="IWO35_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ0316686.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7966..10125
                     /gene="nrdE"
                     /locus_tag="IWO35_001626"
     CDS             7966..10125
                     /gene="nrdE"
                     /locus_tag="IWO35_001626"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ0316687.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10175..11140
                     /gene="nrdF"
                     /locus_tag="IWO35_001627"
     CDS             10175..11140
                     /gene="nrdF"
                     /locus_tag="IWO35_001627"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ0316688.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11229..12368)
                     /locus_tag="IWO35_001628"
     CDS             complement(11229..12368)
                     /locus_tag="IWO35_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0316689.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12677..13390)
                     /locus_tag="IWO35_001629"
     CDS             complement(12677..13390)
                     /locus_tag="IWO35_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ0316690.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13644..14345)
                     /gene="nagB"
                     /locus_tag="IWO35_001630"
     CDS             complement(13644..14345)
                     /gene="nagB"
                     /locus_tag="IWO35_001630"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ0316691.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14579..16111
                     /locus_tag="IWO35_001631"
     CDS             14579..16111
                     /locus_tag="IWO35_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0316692.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16302..>16479)
                     /locus_tag="IWO35_001632"
     CDS             complement(16302..>16479)
                     /locus_tag="IWO35_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0316693.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5108 a         3408 c         2860 g         5103 t
ORIGIN      
        1 catttttttt cattttagca cttccctgga attgctccac ctaaaaaacc acctatagac
       61 attcctgcaa tacaagtagt tgccttggcc caatcgaccg tacatttatt tttagtgcaa
      121 tacactccat ttccataata ttttccacta tgagtggtac ccccatagat aagttgtgtc
      181 tctttaatag acaaaatttt taaatgtttc ataatctaat cacccctaaa ttaatgtttt
      241 tagactatta ttctaacatt cattaaattt caaaaaacaa ttccttcttg aaacgtagca
      301 aaataattcc tgaataagaa tttaatcact tttaagtata taaactatgt atattcttta
      361 atattttttg agaatgcact tcgtacataa taaactttaa tcacattagt ctttcatacc
      421 tacgatcgat ctctttgact cgcacccata aattcataat agtttgtcgg cgtctttgaa
      481 cgtttcgaag atgagctacg cattctcttt ttttatagac aaaaaagata gagacgaaca
      541 tggagcttcc aaatttggat atttgatcca attttggaag ctccatttat ttcgtgtctg
      601 tctttttatt ttttccagct ttcgactaat tcttggttct ctttgatcca gtcttttgct
      661 gcttgctgtg gatcttttcc attgttgatt tccaacataa ctttttccat atctttttct
      721 gaccaatgga aattgtctaa tactttgtaa gcttctggct ggtcttcttt caatcctttt
      781 cgaaccatcg tatggatctg ctcttcgctt cccattccgt tttcaggatc ttctaagtat
      841 ttcagatcat atttggcaaa catccaatga ggtgtccatc ctgtaactac aattggttcg
      901 tgttttttga tcgcttgacc tagtgcaacg gtcattgctc ctgaagaaga tgtctctact
      961 ttccagtctt tcaagttgtc atatttttgg atcgtgtttt cagcagccgt aactacacca
     1021 gctcctggct cgattcctat gattttctta cctgcctgat cggttaactc atcgatcgaa
     1081 tttacatcca tgtaggctgg cactgctaaa ccaacttttg ctcctgtcaa attggcacct
     1141 aggtcttcga cttgatcttt atattgtgca tattgtgaac catgagtttt aggtagccaa
     1201 gctgaaacca tggcatctga ctcgccattt gaaacagatt tccacatgat tgaattatct
     1261 aatggcgtca tcgttacatc atagcccatt tgtttcaata cttcaccgac tacatttgtt
     1321 gaagcaactt ctgtatccca ttcaacataa gataagttga tacgttttgt ttctttggca
     1381 gatgaaaaac tccctatacc aatcgcacct aagatcacta ctactacagc accaatgatc
     1441 agtccttgac gttttttact tttctgtgtt gttgctcctg cagctttttt attgtttggc
     1501 tggttcaaat gctgtgtaaa acggtcaatg atgattgcta agataactaa tgcaaccccg
     1561 ttgacaaatc cattcccgac ttgtgctctt tgcaatgctg ataatacgcc gcgtcctaag
     1621 ccgggtgcac cgatcataga ggcaataacg accattgaaa gtgacagcat tgttgtttga
     1681 ttgatccctg ccatgatcgt acttttagct aaaggcaatt ctagtttgaa tagtttttga
     1741 cgacttgtgc tcccgaatga atcagaagct tctactaatt ctttaggaac ttgtcgaatc
     1801 cctaaattcg taaaacgaac ggttggtggc agagcaaaga tgactgaggc aaatacccct
     1861 ggaaccatcc cgataccgaa aaatgcaact gctggaatca aataaacgaa tcctggcatt
     1921 gtctgcataa agtcaaggat cggtttgata atactttggg ctttactgct ttttgccatc
     1981 aagatcccta atggtacccc aatgatgatc gagatcacac tagataaaag aacaagtgtg
     2041 acagtattca tcaaatcatt ccatagacct tggttataaa taaaccatag accgattaat
     2101 gtgaacaacg taagtcctgg acgtttctta gaaataaaga aagctgctac cgtcaataac
     2161 aagataaaca aaggagctgg aatcactaac agtaaatttg taatccccga catcactgcc
     2221 tgtccaatcg tttgtaaaaa tgaaaatagg ccagaaaaag ttgtcgtaaa ccattctgtg
     2281 atattttcta cccagtttgc cactggtaat tgatagttca atagattatt catgttcact
     2341 cacctcgctt gtttctgcta atgcttcaat cacactacct cggatgacga caccttttaa
     2401 ttttccatcc tctacaactg caagtggagt cggtgagtca taaataatat tgaaaatatc
     2461 acttaccacc atgtctttcc caatcaccgt cacattttca tctaggaagt cgatcaatgg
     2521 acgtttttgc ttcctagctt ctaacgcttg atctgcagtg atgatccctt tcaggtgtct
     2581 ctttctgtca acagccatca gcatgctgac ttcttcatta cgcatccttg tcaatgcaac
     2641 gtttggtcca tcagattcga tattcgtcgt caatgcaggt accatgatgt tttgagccgt
     2701 aagcactttg gaacgatcga cttcttccac aaattcacgt acataatcat ttgctggatt
     2761 ggtcaatatt tcttcacctg ttccgatctg catgatttcg ccgtctttca tcaaggcgat
     2821 tctgtcccca atccgtaaag cttcattcaa atcatgggta atgaaaataa tcgttttttg
     2881 aacattggct tgcaggtcca gtaattcgtc ttgcatttct cgccggatca gcggatcaag
     2941 agccgagaac gcttcgtcca tcagcaagat ctcaggatca tttgctaaag ctctggctag
     3001 gccaacccgt tgttgcatcc caccagataa ctgactagga tattggtctt taaatgaaag
     3061 caaactagaa ttttctaatg ctttttctgc tttttcttgt cgttcttctt taggtacacc
     3121 acggacttct aacccgtatt ctgtattttc caagatcgtt cgatgtggaa acagaccgaa
     3181 gttttggaaa accatattga ttttatgacg acggacttct cgcaattctt ctttgcttaa
     3241 cttcgataca tcttctccat caatatagat actgcccgaa gttggctcaa tcagacgatt
     3301 caatagacgg atcaatgtgg atttcccact cccggaaagc cccatgatca cgaagatttc
     3361 tccttctttt acgtcaaaat tcacgtcata cacaccaacc gttgcaccgg ttttctttaa
     3421 aatctccgtt ttcggctggt gatcattcat catatctaaa gcggcttgtg tctttttacc
     3481 aaatatcttt gttaaatgtt ttacttctac ttttaccaaa caaattctcc ttttttatgt
     3541 aaattcattt ttctttctga aaaaagtgac cacactatgt taacatcatg tagtcacttt
     3601 gtcaaaaatg aaagaaattt gcttgtccaa actgaaaaaa acttggctaa atctttgtaa
     3661 ataaaaggtt tagccaagtt aatatttttt taatatttca cacaatcatt ctgcaagctg
     3721 atgctccggg taaaaaaagt ttgtcatacg ctgtagtgta tattcatttc caataaaatg
     3781 aacgatgtcc cctgccgtaa atttagcata ggggccaggt gaaacaagca gttcttcatt
     3841 atgagaaatt gcgataactg tggcgccagt cgcctgccag atattcaact cgctaattga
     3901 acggtccaga tgcattgctt tttctgataa agataattca aacggaagta aaggattcat
     3961 cttatgtatt cgcttcgttt gattgaccag ctgatccaat aattgagaaa aatgattcag
     4021 ctcatcttgt tgcttctcca cacttgccat gatttctgct tttgtatctt ggatggattg
     4081 tacatcttga tattgcaata ggaaatcttt tgctttttcc ttcgaagcaa caaatgcccc
     4141 acttccatgc cgtacttcca tgattccaag atctaccagc acattgatag ccttgcgggt
     4201 agtctcagga gaagcattgt acgtactggc aagtgttgag cgggcatgga tcttctcacc
     4261 cactttatat cggttttcaa cgatacgttc ggctaaatca acagcaatct gctggtagcg
     4321 cgggcggacg atttttttct ttccagacat tttctttcct ctttcttttt ttactttccc
     4381 tcatcttact atgctctagc aaaataagca aaaaagggga gaatagcctc ttgcaacgac
     4441 tgatcatcgc ttgatctgtt ccttttttac tgggcaagct gtttgacagt catcacaatt
     4501 cttaccggat tttaaacgag tatagacaat tgcgcctgca cttccaaaga ttaacacact
     4561 taataagaat gtcgccatcc tattcctctc cttctaatga caatatagta taaaactggt
     4621 tcttggattt tggctgacgg aacaaaacat aaacgagtcc tatcatcagt aaaatagcaa
     4681 gatacgtccc taacgctacc tgtccaccct ccaataagtg tcccaactga taaatcacaa
     4741 aactaactaa ataagctaaa ccgcattgat agcctatggc gatccaagtc catttcttgg
     4801 cattcatttc ccgatggata gccccaattg ccgcaaaaca aggcgcacaa agtaaattga
     4861 aggctaacaa tgaataagct gctgcgggag tatatgtgtg ttgtagcact tgccagattt
     4921 ctctgccatt ttcggatact tccgcatgac caaacaaaat accaaatgtg ccaataatat
     4981 tttctttagc gaccaatcct gtaatcgctg cgactgttcc ttgccagttt ccccaaccaa
     5041 gcggctgaaa aacaactgcc aggattcgcc cgaaaaaggc taagatactt tggtcttcag
     5101 aaacaggttg agcataaaaa ttataagaag agctgaacca gatcacgata ctagaaacaa
     5161 agataatcgt tcctgctttt ttaacaaaag acttgctgcg gtcataggtt tgtcttagga
     5221 ccgctgacca ttgaggaaaa tgatatgcgg gcaattccat gataaaagga gctgtatctc
     5281 cagcaaacaa acgtgttttt ttcaagctga ttccagagaa aataattgca gtcataccaa
     5341 gaaaataggc agaaggagct acccaagatt gatgaggaaa aaaggcccct gctactaagg
     5401 caataatcgg caactttgca gaacaaggca tgaaggttgt gaccatgatc gtcaaacgac
     5461 gatcttgctc gttttcaatc gtgcgactgg ccatgacgcc tggcactcca catcctgtag
     5521 caatcagcat aggaatgaag gattttcctg aaagaccgaa tttcctgaaa agtcgatcca
     5581 tgacaaaagc gatcctcgcc atatatccgc agtcttccag aaagctcaag cataaaaaca
     5641 atataatcaa ttgcggtaga aatcctaaca ctgctcccac gcctgcgatg atgccgttca
     5701 agatcagctc ctgcatccat cccgctactt gccattcagc taacaatctg ccaacatgtt
     5761 ctggtaacca tgtaccaaac aatgtgtcat tgatccaatc tgtccccatt gttcctactg
     5821 tttgaatagc cagataatag atcagccaca tcacaaatgc gaagatcggc aaggctaacc
     5881 aacgattcgt tgcaatctga tcgattttgt cactcatcga taattggaaa gaatcatttg
     5941 acacaacaca gagtgtacat agttgggtaa taaattcata acgttcattg accagaatcg
     6001 tctcactatc gtcgtccaat agtttctctg ttaactggat cagttgctcg atctcttttt
     6061 gctgagaaac tgctatctcg taatcctctc ttaccttctg atctcgttca aaatatttca
     6121 ttgcatacca gcgtttcttg ccgctttgtt ctcctagtac atcttcaatt tcatgtaaag
     6181 ctgcttctaa acgtggatca tatgtacagg gaacaggaat agaaggccgc ttttttgcag
     6241 actcctgaat cttttcgatt agtttttcga ttccttgctt tttcaatacg ctgattggta
     6301 aaactggtac accaagacca taggaaagct tttctagatt gatttttttt ccttcttttt
     6361 tgatgacatc catcatattc agaccaatga tcataggaat gtctaaatcc aataactgcg
     6421 tagttaaata aagatttcgt tctaaattgg tagcatcaat aatgttgatg atcagttcag
     6481 gctgttcctg aaccaaataa tctcttgcaa tcacttcttc tggtgtataa ggagacaaag
     6541 aatagatgcc gggtaggtcc tgaatatgaa tgttcggatt ttttttcaac gatccttctt
     6601 ttttttctac tgttacgccc ggccaattcc ctacatattg attcgaacca gtcaagagat
     6661 tgaacaagct agttttcccg ctgtttggat ttccgactaa cgcgatattc attctttttc
     6721 ctccctttgt atcaagatta gttcggcctc tgactttcgt aacgtcagtt cataccctcg
     6781 cactgtcagt tcgattggat ctcccagagg ggctagtttt cggataacta tttttacatt
     6841 tcgcgtcaat cccatatcca ttaaccgtcg cttcactgcg ccttgtccgt ggattgctac
     6901 gacgatccca gtctctcctg cttttaattg atcaagagaa gtccagcttt cagaggaagc
     6961 agtcttttta ttgacaaaga tttgacctag tatttcccga cttactgcca ctcgatgact
     7021 ttttaccagt aaaatggcat tgttgcctga taaattgacg acgacgattt tacttccggc
     7081 aacgatcccg atattttgca aatggcgttt tgttttttct tctgtttcga ttcgttcgac
     7141 ttgataaacg aaattctgct ttgcctgcgc taaggtctgc atattgattc ctctcttgtg
     7201 aaataaatca tgtattaaaa tcattcatta tatgttctaa ttttcttacg tgttacgcaa
     7261 ataaatgaga atcattctca catagaatac tctcggtaat tgagaatgtc aattatactt
     7321 gtgatattat gagcgaatct ttagtattga tagataaaaa tgagaaaaaa atatttcttc
     7381 atagttttcc acaatttttt ctctattaaa tttgctattt tcccacacga tacttgtttt
     7441 cagctctttt ttcaaaaaca caaaacttag tattccacag tttccacaac acaatatgta
     7501 gtgtttgttg tttgactttt ttactaagag ttttatacta tctattatag ataataagaa
     7561 tgtgttagag gaggcactca ccatgaacgt aaaaatcttt tctaaaaata attgtatgca
     7621 atgtaagatg gtcaaacggt ttttaacaga aaatcaaatt gcttttgaag aagtcaatat
     7681 cgatgaacag cctgaagcag tagactggtt gaaagcacaa ggattccaaa gtgttcctgt
     7741 cattacttct gatgcagaaa cagttatcgg attccgtcct gatcagttgc gtaaacttgc
     7801 cagctaacat gactaaaaga atcaaaacgt gatacatgga tttgaagggc ttgagacagc
     7861 accagttttt gtcgcaaact tccaaatcct tttttaaagc ttaaaaacaa tgcaatgatt
     7921 tgcaatcaag catcatcaaa tacgaaatga aggttgggcg tacgtatgag tcttaaaaat
     7981 ttacaagatg tcagttattt caagctgaat aatgaaatca atcggccagt caatggtcaa
     8041 atccctttga ataaggataa agaagcttta gttgcctttt ttgaggaaaa tgtcaaacca
     8101 aatacgatga cttttccatc tattatggaa aaaatcaatt acttgatcga tcaagaatat
     8161 atagaagcag aattcattca attgtattca ccatcattta ttgaaaagct atatcaattt
     8221 ttggacgagc aacatttttc tttcaaatct tttatggctg cttacaaatt ttattctcaa
     8281 tatgcgttga aaaccaacga cggttccgct tatctggaaa cttatgaaga tcgagtagcc
     8341 ttcaatgcgc tttactttgc gaacggggat gaagaattag cacttgctct tgcagacgaa
     8401 atgatccatc aacgatatca gccagctaca ccttctttct tgaatgctgg acgtaaacgt
     8461 cggggcgaat tggtgtcttg tttcttgact caagtcactg acgatatgaa ctctatcggg
     8521 cgctcaatca actctgcttt gcaattgtcc cgtattggtg gcggcgtagg gattacttta
     8581 tcaaatcttc gtgaagccgg cgctcctatc aaaggatatg agggtgctgc aagtggtgtc
     8641 gttccagtca tgaaattatt cgaagatagt ttcagctatt ctaatcaatt aggtcagcgt
     8701 caaggagctg gcgtggttta tttgaacgtt ttccatccag acattattgc tttcctttca
     8761 gcaaaaaaag aaaatgcaga tgaaaagatt cgcgtaaaaa ctctttctct tggcgtgatc
     8821 gtacctgata aattttatga attgacacgt aaaaacgaag atatgtattt atttagtcca
     8881 tatagtgtcg aaaaagaata tggtgtccca ttctcttatg taaatattac agctgagtat
     8941 gatcatctag ttgcaaatcc aaacattcgc aaacaaaaaa tcaaagcgcg tgatcttgaa
     9001 aatgaaatct caaaattaca acaagaatct ggctatcctt acgtaatcaa tattgataca
     9061 gcgaaccgcg agaatccaat cgatggcaag atcatcatga gtaatttatg ttcagagatt
     9121 ttacaagttc aaacgccttc tgtgattaac ggcaaacagg aatatgaagt attaggaaca
     9181 gatatctcat gtaacttagg ctcgacaaat atcgtgaatt tgatgcaaag ccctaacttt
     9241 ggtcaatctg tgcgtacgat gactcgtgca ttgacattta ttacagatgc atctgatatc
     9301 gatgtcgtac caccgatcca aaacggaaat aagctgaatc atacgattgg attaggtgcc
     9361 atgggcttac acacttatct tgcaaaagaa caaatggaat atggctcaga ggattcatta
     9421 gactttacaa atatttactt catgctgttg aattattgga cacttgttga aagcaacaat
     9481 atcgctcgtg aaagaaaaca agttttccat aattttgaaa aatcagctta tgctgacggc
     9541 acttatttcg aaaaatacgt aactggagaa ttccagccaa aatcagaaaa agtcaaagca
     9601 ttgtttgaag gcatttttat cccaactgct gaagattgga atgctttgaa acaagcagta
     9661 atgaaagatg gactgtatca tcaaaatcgc ttggcagtag caccaaatgg ctcaatttca
     9721 tatatcaacg atacgagtgc aagtattcat cctatcactc gcatgatcga agaacgccaa
     9781 gagaagaaaa tcggtaaaat ttattaccca gcgccttatc tatccaatga gacgattccg
     9841 tattacactt ctgcttatga tatggacatg cgtaaagtaa tcgatgttta tgcaacggca
     9901 caacaacacg tcgatcaagg catgagtttg actttattca tgcgttctga aatcccagaa
     9961 ggcatttatg aatggaagac aacaacgaag caaacgacac gtgatttgaa tattttaaga
    10021 cattacgctt tccacaaagg aatcaaatcc atttattacg tacgtacctt tactgatgat
    10081 gcagaagaaa tcggaagcaa tcaatgtgaa agctgcgtga tctaatatca tagatagatt
    10141 cgcttattca tattttaatt aaaggagcat tctcatgtca gaaacatatt atgaagcaat
    10201 aaactggaat gaaattgaag atatcattga taaatccact tgggagaagc tgacagagca
    10261 attttggcta gatacacgta tcccactttc aaatgaccta gatgattggc gtacattgtc
    10321 tcaattggaa aaagatacag taggacatgt atttggcggt ttgacactgc tagatactgt
    10381 ccaatctgaa agtggcatgg atcagttgcg taaagatgtc cgcactcccc atgaagaagc
    10441 cgttttgaac aatattcaat tcatggaatc cgttcatgcc aaaagttatt cttctatttt
    10501 tagtacactg aatacgaaaa aagaaatcga agaaattttt gactggacaa atacgaatcc
    10561 ttatctgcaa aaaaaagctg agcgtatcaa tgaaatctac aaacacggta caccgctaga
    10621 aaagaaaatt gccagcgtct ttttagaaac attcttgttc tattcaggtt tttatactcc
    10681 tctttattat ttaggaaaca acaaattggc taacgtagca gagatcatca aactaatcat
    10741 ccgagatgaa tctgtacatg gaacctacat cggttacaaa ttccaattag gattcaacga
    10801 gttacctgaa gcagaacaag aaacgttaaa agactggatg tacaatcttc tttatgagct
    10861 ttacgaaaac gaagaacgtt atacagaaga attatacgat ccaatcggct ggacagaaga
    10921 agtcaagaca ttcctacgct ataatgccaa caaagcattg atgaacctag ggatggatcc
    10981 actctttcca gatactgcca atgatgtcaa cccaatcgta atgaacggga tttcaacagg
    11041 cacaagcaac catgacttct tctcacaagt cggaaacggc tatttgttag gtcatgtaga
    11101 agcgatgaaa gacgacgatt atttgattgg attggaataa ggatttattt taattcaaaa
    11161 atttttcgaa aaataaaaag agtttagcgt acagatattt taaatgtaca ctgaactctt
    11221 ttttatgctt attcacttgt ttcttccagc acttttggac tgtctaactt ttccgaaaca
    11281 tgttccattc ttctcaataa atagattttc cggatattta atattatcgt cttaatcact
    11341 gcatagacag gtatgcaaat cagcatgcct aatattcctg cgacacttcc tgctccaatc
    11401 aataccaaga tgatcgtcaa aggatggata ttcattgatt tcccaaataa caacggcttg
    11461 atcaaatttc catctaattg ttggatcact aaaatcgaca acgccatata caaagcttga
    11521 aatggagaga taaacaaacc gacaatgatt gctggtaccg ctccaataaa agggccgaca
    11581 tacgggataa tattcgtgat tccgcaaatg accgcaagca acaagctata aggctgatcg
    11641 aatattctca ttgccaaaaa gctcattaca ccaataatca aagcatctaa gatcgtactg
    11701 ctgatatagg cagataatgt ttcgtttaat tcttttaccg tttggcgtaa ctctttacga
    11761 atcgctttcg gaaagaagtg agacaacgca tcaataaatt tgtgcccatc cttgaacata
    11821 aatagcaaaa taaatggaac ggtaaataac aaaataaaaa attgcatcat aaatgaaatg
    11881 atttttgaga cacttgcaga taaactgact aatatgatat tgaacaaatt actaagagat
    11941 aaatttgctg atgctaattg ttcttccaaa ttgaaatttt taaactcttc ccgctgcgcc
    12001 aattcattta accatttgct tgtttcatct gcatacattg gcaagctctg ggtcaattga
    12061 attgtctgtt ccattaactg aggcactaca ttcatgacag ttaaaacaac taaaatgatt
    12121 acgaccaaaa acgtcaataa aaaaccgaac actctcggga tccgatgctt ttcgagaaaa
    12181 acaacgattg gatcgaacat ataatagata aaaccagcaa caagcaatgg taaaaaaatt
    12241 gccgaaagca ttttcaatat tggactaaaa atattcggca tttgtaaaat aaagtacacg
    12301 ccgatgatcg tgagtactag ctccactgtc cagaaaaata attttgattg tttaaaacgt
    12361 gattgcatac atctcctcca aggtcctttg atacttctat tctatcattt aacaaagagt
    12421 tacgcgctga gattttcatt tgcttaaaaa aaacaaaaaa aataaacata ttttaatgag
    12481 tagacgagta cttaaacaaa aacacggttt ggagtagtga caaaaattat actgcagtcc
    12541 ctttttccaa ataaacggtg ttcaaaagct tgcatcttcg taataagccc agccaaacga
    12601 aaaatataag gaactatttt tatttggctg ggcttattac ttgatgtagg acatctattt
    12661 tacaacctca gatcatttat tgcgcaatac tgactaaatg caactttgtg atatttttgt
    12721 gtacggacaa ctcctcgatc acatcggcat aagtgagccc ttttggaaga tcaatcgtat
    12781 agatattttt gtacactcgg tagtcatcaa cgaattttac gtcaaaattc acatcttcga
    12841 ttacgatatt tttctcttca aaataatcgg taagaaaatc tttggtttct tttctatgga
    12901 taaattggat ttctaacttt ttagtcgtag gtacatggat cacgcgtttc actactgtga
    12961 gtgcaatagc aatcccgata aaactgatga tcgcaatgga atagtaaccc atgccgatgg
    13021 cgatccctaa tccagcaatt gcccataaag aagcggcagt cgtaagtccc gtcaccgatt
    13081 gtttcgtgac gatgatcgtt cccgcgccta agaaaccgac accactcacc acttgagcaa
    13141 tcagccttgc ttcatctgaa cgaatcgttc cagcaagctg tggatactct aaagcatctc
    13201 ttaatgcacc actagcgatc tcaacttgga tcaatgcaat gatacatgct cccatacaaa
    13261 ctaaaatatg cgtacgcata cctgctggac gatttttata ttgtcgttca aaaccgattg
    13321 ctccgccaaa tagcatagcg atccctaaac gtaaaataat ctctggaatt gtcaatacca
    13381 tatcattcat ctaaacaccc tccagtccaa tatatcatgt ctgaaaggag cagacaaaaa
    13441 aagtgcaatt tttacctctt acgaaaaaag gactgtgaca aaatttttgt catagtcctt
    13501 tttatgaata aacggtgttc aatcagctga ccctcggtat tactcaacaa acggcaaaat
    13561 atggggagct atttttcctt tgtcttctct aatactcagg tcaaaacgct gatttcacaa
    13621 cctctttagc ctactattga aaattacaac ttacttgctg cttcttcatc gatgatcaca
    13681 acaacattgt cgtgattttg caatacactt gctggcaaat gattcgttac aggtccttca
    13741 atcatccctt tgattgcatc agctttgctt tcgccaaatg ccatcaagac gatttcttta
    13801 cttttcataa ttgaaccgat tcccattgaa acagcttttg ttggtacatc ttctgcttta
    13861 tcgaagaatc gtttgtttgc attgattgtt gattctgtta agtcaactac tgaagtcgtt
    13921 ccgtcaaatg aagcaccagg ttcattgaaa ccgatatgtc cattttgccc gattcctaag
    13981 atttggatat cgattggatg tgcatcgatg atttgatcat actttttgca tgctgcttcc
    14041 agatcttctg cttttccatc tggtacaaac gtttctttga atggtttttt atcaaataat
    14101 tggacatcca tgaaatgacg gtagctttgt tcatctgaac cgcctagtcc aacgtactca
    14161 tctaagttta cagaagtcat ttcactgaaa tccaaatcgc ttgaagtcat ttctttatat
    14221 aaagtaactg gtgtgcttcc tgttgccaat cctaatactt ttgcaccttt gttcatacct
    14281 tctttgatga tttcaaatgc tttttttccg ccttcttcag cgttttttac acgaatgatt
    14341 tccatagtga ttgctccttt atattttggt atagcccaat tatagcattc tttttttctt
    14401 tttacaataa ttagtataga ccaaattgca tttttttcat tttcttgaca ataaatagga
    14461 atccgtgtta ggtttcataa gatggttcaa tcaaacaatc catgttgcat gaaaaccaag
    14521 caagtgattc actgtggacg ctttatgtct gcagtttttt tatgaaaaag gagttattat
    14581 gagtatactg acattaaaca acattaccca gcaatttggc gacaaaatcc tctatgaagg
    14641 tgtcgatctg caattaaatg ctggcgaaca tcttgggctg attggacaaa acggagccgg
    14701 taaaagtaca ttgattaaaa tcatcactgg tgagatctta ccagatgacg gacagatcca
    14761 ttggcaaaaa aatattcaca aaggttacct cgatcaatat gtggaagtcg atgaaacatt
    14821 aacaatcgaa gaatttctaa aaactgcgta cgcaaaagaa tacgaaaagg aagaaaaaat
    14881 tgcttttctt taccaaacat atagcgagac cttcgatgat tccttgctgg aaaaagctgg
    14941 gaaactccaa acggaattag accaaggagt gttttatcaa atcgacacct tagttgctga
    15001 gatgagcagc gggttgggga tcgatgtctt aggtttagat tcactgttga aaaatctaag
    15061 tggtggacaa cgttcaaagg tgattttagc aaaactactt ttagaaaacc cagatgttct
    15121 gatcttagat gaaccaacca atcacttaga tgatcagcat atccaatggc tgacgcagtt
    15181 tcttcaggat tttgacggtg cgtatatcgt gatttcccat gaccaagaat ttcttgatca
    15241 gatcaccacc catatcgcag acatcgagtt tggaaagatt actaagtata ctggccatct
    15301 aaaacaagca ttgaaacaaa aggaacaaaa tcgcgaaagc tatttgcgcc aatatcatgc
    15361 tcaacaaaaa caaatcgaaa agaccgaagc ttatattcga aaatacaaag ccggttcccg
    15421 atcaacgatg gcaaaaagcc gacaaaaaca attagataaa atcgaacggc tgacgcctcc
    15481 agcttcagca agtaaagctg tctttgattt tccattctcg cctattgtca cgacattagc
    15541 agtggaaaca actgatttag tcatcggata tgatcgaccg ctacttgcac ccatcaattt
    15601 gaccatccgc tttggtgaaa atattgccat tcgaggcttc aacgggattg gaaaatctac
    15661 tttattaaaa acactgatcg gtgagattga aaaaataagt ggcgatttcc acttcccaga
    15721 taacacaaaa atcaattact tttcacaaga tctgtactgg gaaaatccat tagaaacacc
    15781 actacaatat ttaagcagtc aatttccaaa agcgactatc aaagaattac gtcgccaact
    15841 agctaaagca gggttagtta atcaattagc aagtgaacct ctcactacat taagtggtgg
    15901 ggaacaaact aaggtgaaac ttgcacagtt gacaatgaac caaggtaatt tactgattct
    15961 cgatgaaccg acgaaccata tcgatcaaga aacgaaagaa agcttacaag acgggataca
    16021 gaaatttcca ggaacagtaa tcatcgtctc tcatgaacaa gaattttatc aagatcttgt
    16081 gcaacgagtc attgagattg aaccaaaata aacgtttgat tgaatagttg aaaaaaagga
    16141 gctgtgacaa aagttttgtc acaactcctt cttctgaata aatggtggcc aattagctga
    16201 ccttcggtat tagctcaaaa aacggcaaaa tatgaggagc tatttttatt tggctattct
    16261 tattacttga tgcagaacag cttttcacaa cctcttttcg tctatttttt attttcgaga
    16321 tttctctttc ctttaaaaac aactgtttga aagaagaacg cacatccttg aaaaacaagc
    16381 ccgctaaccg ttacacctaa ccagccatac tgctgccaca ttgtcgtccc tagcaatgaa
    16441 ccagccgcac ctccaataaa gtacataaac ataaataca
//
LOCUS       DACECZ010000056        16443 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.056, whole genome shotgun sequence.
ACCESSION   DACECZ010000056 DACECZ010000000
VERSION     DACECZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.056"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            377..1507
                     /locus_tag="IWO35_001633"
     CDS             377..1507
                     /locus_tag="IWO35_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0316694.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            1599..2354
                     /locus_tag="IWO35_001634"
     CDS             1599..2354
                     /locus_tag="IWO35_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ0316695.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            2412..4451
                     /locus_tag="IWO35_001635"
     CDS             2412..4451
                     /locus_tag="IWO35_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ0316696.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4560..5711)
                     /locus_tag="IWO35_001636"
     CDS             complement(4560..5711)
                     /locus_tag="IWO35_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0316697.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(5865..6395)
                     /locus_tag="IWO35_001637"
     CDS             complement(5865..6395)
                     /locus_tag="IWO35_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316698.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(6494..6844)
                     /locus_tag="IWO35_001638"
     CDS             complement(6494..6844)
                     /locus_tag="IWO35_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ0316699.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(7001..8530)
                     /gene="ahpF"
                     /locus_tag="IWO35_001639"
     CDS             complement(7001..8530)
                     /gene="ahpF"
                     /locus_tag="IWO35_001639"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ0316700.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(8540..9103)
                     /gene="ahpC"
                     /locus_tag="IWO35_001640"
     CDS             complement(8540..9103)
                     /gene="ahpC"
                     /locus_tag="IWO35_001640"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ0316701.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(9247..9909)
                     /locus_tag="IWO35_001641"
     CDS             complement(9247..9909)
                     /locus_tag="IWO35_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0316702.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            10093..11739
                     /locus_tag="IWO35_001642"
     CDS             10093..11739
                     /locus_tag="IWO35_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ0316703.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            11730..12812
                     /gene="uxuA"
                     /locus_tag="IWO35_001643"
     CDS             11730..12812
                     /gene="uxuA"
                     /locus_tag="IWO35_001643"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0316704.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(12920..14473)
                     /locus_tag="IWO35_001644"
                     /pseudo
     CDS             complement(12920..14473)
                     /locus_tag="IWO35_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            14621..15762
                     /locus_tag="IWO35_001645"
                     /pseudo
     CDS             14621..15762
                     /locus_tag="IWO35_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(15888..>16443)
                     /locus_tag="IWO35_001646"
     CDS             complement(15888..>16443)
                     /locus_tag="IWO35_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0316705.1"
                     /translation="IEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQF
                     VVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGIR
                     DVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIPI
                     FTFFLDVLKVKKIPNHLPKETDIP"
BASE COUNT         5556 a         3007 c         3066 g         4814 t
ORIGIN      
        1 tcaaattcca ttgcctaatc attaggaaaa gtctatcatg aactcgtaaa tagaatatag
       61 taataacctt gttaggtgag gctcctatac aaacataggc tgttgcccag aaacgtcgaa
      121 agtcgccaat gggtaaaaca gaaactgttg gattaaggct ttttccaata gctaagaatg
      181 ttttctttac gttgtatagt gctaaaactc ggacgatgag atgatgctag gtcagaattc
      241 ttgttcgaaa aggtttctga ccttttcttt atatttttgt gactgactta tttgatgaga
      301 caaactcgct cctcacctga caagtggatt gctgtgtgag gaggagatgc ggtgaaaaaa
      361 gatcgattgg tggattttgg gtccttattt gactttgtcc atgatcggtc ttttagaagt
      421 atacagtgcc agttcttatc gtttattgca agccgatgaa aatacaaaaa gtctattatt
      481 gcgtcaacta attttcatat ttttgagttg gagcgtgatc ttcttagctc gttcagtcaa
      541 actacactat ttacttcacc ctaaaatagc aggatacggt ttagccttat cgattttctt
      601 tttagtatta gtaagaatag ggatattcgg tgtcactgtc aacggcgcac aacgttggat
      661 ctctctgttt ggcattcaat tccagccttc tgaactggca aatctttttt tgatttttta
      721 tttaagctgg ttttttcgtg acggaaatag tagcccaaaa gatctaaaaa aaccattcct
      781 gattacagta ggtataactt ttctgatttt atttcagcca aagattgctg gagcattgat
      841 gatcctttcg attgcgtggg tcatattttg ggcagcggcg gttccattta aaaaagggat
      901 ctatctaatc gttacttttt ctgcattgct gattggagca gcaggcgggg tattatattt
      961 aggaaataaa ggttggcttc cacagatgtt taatcatgct tatgaaagga tcgcgacttt
     1021 aagagattct tttatcgata gtcatggtgc gggctatcaa atgacccatt cgttttatgc
     1081 actatataat ggtggcatat ttgggcgggg attaggaaat agtatcacaa aaaaagggta
     1141 tcttccagag acagaaacag attttatttt ttcgattatt acagaagagc ttgggctaat
     1201 cggtgcattg tgtgtgcttt tcttattgtt ctcattatgc atgagaatct tttgtttaag
     1261 cagtcgctgt aaaaatcagc aagctggatt gtttctttta ggattcggaa ctttgctatt
     1321 cgttcagacg ataatgaacg tgggaagtat cgcggggctg atgccaatga ctggtgtgcc
     1381 attgccattt gttagctacg gaggaacaag ttatctgatc ctttctttag gaatcggaat
     1441 cacgttgaat atttcttcaa agatacaggc cgaagagttg gcgctctatc gacctgaaaa
     1501 acaataaaga gaaactgaga aactctcagt ttgaattatc ataaagtaca atcttttatt
     1561 tttcaagcga gaggttcttt tagaaagagg aattcgcaat gaaagtatct caaaaaatca
     1621 tattatctat tttgttactg cttacattag tagttggcta tttttcgatt gagtttatcc
     1681 atggattttc gtctgcaaaa cagagttcaa ctgtgaaaaa ggtagagccg aaaagtatcc
     1741 ctaccacact aaatgtggct ttgattggtt cagatgctcg ttcgaaagac gaaaacggtc
     1801 gctcggattc acttctggtt gcacaataca accagaaaac acaacaagca aaactgatct
     1861 ctatcatgag agattcatat gtcgatattc caggttacgg aatgaataag ataaatgcag
     1921 cttactctta tgggggagtt gatttattaa atcaaacatt aaaggaaaat ttcaaatttg
     1981 aagctcccta ttatgcaagt atcacatttc aagattttat cgattgcgtt aatgaactgt
     2041 ttcctggtgg agtcaagatc gatgcagaaa aatctctaga tttagatggc gtatatataa
     2101 acaaaggaga gcaagtgatg gatggtaata ctttactcca gtatgctcga tttcgggaag
     2161 acaaagaagg agacttcggg aggatcagaa ggcagcagca agtgatcaaa gctatctctc
     2221 agcagctgaa agacgtaact tcaatattca agctacccaa agcggttggg aaattacttg
     2281 gtagtatcca aacgaacctt ccagaaagtg tgcttctgga ttgtggtatg gatttcctca
     2341 aagacatatg ttagaataaa caggtataaa tagtgaaata aaggaatgac aagcaagaga
     2401 aggaggaaaa aatgaaaaga agtgacaagc acggcaaaaa tcgaacaggc gcttatattg
     2461 ccggcgcagt gatcttaata gcagctgcgg gtggcggtta tttttactac cagcactatc
     2521 aagaaaccca agcagtagaa gctggagaaa agacagttga gcaatttgtc caagctttga
     2581 acaagggaga ttataacaaa gctgcagaaa tgacatcaaa aaaggcggca aataaaagtg
     2641 cattatctga aaaagagatc ttagataaat accagaatat atacggtgct gctgatgtca
     2701 aaggacttca gatatcgaat ctaaaagtag ataaaaaaga cgattctact tacagttttt
     2761 catataaagc aaagatgaat acctcattag gtgaattgaa agatctttct tataaaggaa
     2821 cattagacag aaatgatggt caaaccacga tcaactggca acccaacttg gtttttccag
     2881 aaatggaagg aaatgacaaa gtaagcctga ccacacaaga agcagcaaga gggaatatta
     2941 tagatcgaaa tggtgaacca ttagcaacaa caggcaaact aaaacaatta ggagtcgttc
     3001 caagtaaact tggagatgga ggcgaaaaaa cagccaatat caaagcgatt gcttcctcct
     3061 tcgacttaac agaagatgct atcaatcagg cgatttcaca aagctgggta caacccgatt
     3121 actttgtccc attgaaaatc attgatggag caacgccaga acttccagct ggagctacca
     3181 ttcaagaagt agacggcaga tattatcctt tgggtgaagc agctgctcag ctgattggtt
     3241 acgtgggaga tatcacagca gaagatattg ataagaatcc agaattaagc agtaatggaa
     3301 aaatcggtcg ctcaggtttg gagatggctt ttgataagga tcttcgtggg acaacaggtg
     3361 ggaaattaag cattacagat gcagacggtg tagagaaaaa agttctgatc gaacatgaag
     3421 ttcaaaatgg caaagatatc aaattgacaa tcgatgcgaa ggcacaaaaa acagcttttg
     3481 acagtctagg aggaaaagct ggatcaactg ttgcgacaac gccaaaaacc ggtgatcttc
     3541 ttgcgcttgc tagctctcca agctatgatc caaacaaaat gacaaacggg atctcacaag
     3601 aagattacaa agcttatgaa gaaaatcctg aacatccatt catcagccga tttgcgacag
     3661 gttatgctcc tggatctacg tttaaaatga ttacagcagc aatcggtctc gacaacggca
     3721 ctatcgatcc aaatgaagtg ttgacgatca acgggcttaa atggcaaaaa gacagttctt
     3781 ggggatctta tcaagcaacg cgtgtcagta gtgatgtatc acaagtagac ttaaaaactg
     3841 ctttgatcta ttccgataat atatatgcgg cacaagaaac gttgaaaatg ggtgagaaaa
     3901 aatttcgtac aggcttagat aaattcattt ttggtgaaga ccttgatttg ccaattagta
     3961 tgaatccagc acaaatttct aatgaagata gctttaactc agatatcttg ctagctgata
     4021 ctggatatgg acagggcgaa cttctaatta ctcctatcca gcaagcagca atgtattctg
     4081 tttttaccaa caatggcaca cttgtctatc ctaaattgat tgcagataaa gagacaaaag
     4141 ataagaagaa tgtcatcagc gaaacagcag tacaaacgat cgtgccagat ctgagagaag
     4201 ttgtgcaaga tgtaaatggt acagcacatt ctctttctgc tttagggatt ccgttggcag
     4261 cgaaaactgg tacagcggaa atcaaagaaa aacaggatgt aaaggggcaa gagaacagtt
     4321 tcttgtttgc tttcaatctt gataaccaag gttatatgat ggtcagcatg ttggaaaata
     4381 aagaagatga tgattcagca accaaacgag catcggaact attgcaatac ctcaaccaaa
     4441 attatcaata aaaaattgct ttttaagtac agtaaaagga ctgtggcgtt atcgtcacag
     4501 tccttttttc aaataaacgg ttttcacgat gtctctttca caacttcttt ttgatttact
     4561 cacacttcat aaactttttt tgtaaaatat ttgagaacaa gtggagaaat aagtgtactt
     4621 aataaaatca cgataactaa aggtgaatag tactgtggtt cgatcaattg actttgctgt
     4681 ccaatttgta aaatgatcaa agccatttct ccacgcgaaa tcataccagc accgaccatc
     4741 aatgaactat gttgagaaaa accggcaatc tgagcaccaa aataacctcc aagcaatttg
     4801 gtcaaaatcg ctacaagcgt caatatagaa ataaagagta gttgttcact aaagtcagaa
     4861 aaatcaactt ctaatcctac actgacaaaa aatactggta taaatactgc atatcctaaa
     4921 gcctctacat tgtggtatac ttcttcttta acctttgtct gactgacagc tatcccagca
     4981 aaaaaagcac caatgacaga gctaagccca acaagatccg ctacgtaaga cattcctaaa
     5041 caaataacta gcgatacgat gataatagaa gaatttgcgt acactttttc tgcaaatgaa
     5101 agtaggaaag gcgcaatcca tttgactaat aagaaaataa aaacaaaata aattagctgt
     5161 tcaatcaaaa caagcggtaa agaagcattg ctagaagctt cccccgttag aaaagatagg
     5221 ctgaaactta gaactaaaac gactaaaatg tcatctacca cagaagcacc caaaattgtt
     5281 gatccttctt ttgtattcac tacgtttagt tcttttaata cttctactga aatactgaca
     5341 gaagttgccg caagaataat cccaaagaaa atcgcttcat ttcccgaaac ttggaatgct
     5401 ttgcctgtca accagccaaa aacaaccggg aagatgatcc caagcaaagc aacgaacata
     5461 ccaggtcgga aatatttttt caaaagagat aaatcacttt ccagaccagc taaaaacatt
     5521 aataagatca caccaatctc cgaaaagtca tggactagaa tatcaggttt gacccaattc
     5581 aatccagctt ggcctagcaa aacaccaaca agtaattgtc caatcactgc tgggatacct
     5641 aatctccgcg agatatgtga gccaatcgtt gttgaaatca atatcaggca taaaatacca
     5701 ataaattcca ttctcatcat tcctttgtat aaaatatagc ccgaacttag cataacatac
     5761 attattgaaa aaataggaaa atatactcac aaaaaaacca acaatccaaa agattttttt
     5821 ggattgttgg tttttatacg gtctatttta ataactaaaa atgattaacg tatagtcatt
     5881 tttcttattc tgatgaaatt gataatctcc tggatgccca tgaaaatcaa aataaagccg
     5941 aatatttgga atagaagcat caaacttttg aatggattga acaataagac gaatccgcca
     6001 atcaacaaca gaatactact ataaacactc caacttttag cttttgtatt catgctgaag
     6061 aacaactgga acaagctgtt gatgataaca gctaatccaa gaagaatcgg caggatagaa
     6121 acgattgcag gtgcaaaagc atagacgatc agagctaaga tcaggaaaaa aagaccactg
     6181 aacagaccga tactccaaga acctgttttc cttttaaaac ggtaagcctc aaaaagattc
     6241 aacaacccaa gcaaaacaag ataggcggtg atcagataac cgataaaatg aaaaacagca
     6301 gaagggttta gcacaatcgc aatcccagaa ataatataaa cagcagctct taataaggcg
     6361 tatttttgaa tattttgttt aatcgtctca aacatataag aaaacctcct ttgacttatt
     6421 ttaacgtgaa ctagtcattt taccaaatta aatgttacaa aatgagcgaa ggagaagaat
     6481 atctctggaa aaatcaactt ctgaggatct tttccatttt tttgcctttt gctagctcat
     6541 cgatcatttt atctaaataa cgaatttctt tcatcaagtt atcctcgatg tcttctactc
     6601 gtacaccaca aacaacacct gtaatcaagg aacgtgaagg attggggaca ggggcttccg
     6661 caaaaaattg ttcgaaagtc acttgttttt gtagttgata ttctagttct gtttgggaat
     6721 aacccgtcag ccaacaaatg atttcatcta cttcctcttt tatacgatct tttctctccg
     6781 cttttgctac atacatagga taaacgcttg caaaacttgt cgtaaaaata cgattttgcg
     6841 ccataatcag tcacctttct ttttttcaaa ttatttacta cttatagtat ataagaaaaa
     6901 gagaagaaaa agcgattatt caaaaaaaca aaaaggattg tgacaaaatt cttgtcacca
     6961 tccttttttc cgctctctga ctttttacaa tcttcatagc tcaagtgcgc atcagataat
     7021 cgaatgctcc taaagctgca gtagctcctg aacccattga aacaatgatt tgtttgtatg
     7081 gactatctgt acaatcacca gctgcgaaaa cgccggggat attcgttgcg ccatttttct
     7141 cggtcactat ttcatttctt ggattcattt cgatcgttcc atctagccaa tccgtattag
     7201 ggagtaagcc gactagaatg aatactcctg aaacagctag tgtatgcatc tccatcgtac
     7261 ggcgatcgat ataggagaga gattctacct gattcgtacc gctgatttct ttcgtcgctg
     7321 catgtttgat aacagtgaca ttcgcaagag agactagttt atcctgtaag atttgatctg
     7381 cttttaattc tggtaagaat tctagaacat atacatgttt tgctaaccca gccaaatcga
     7441 ttgctgcttc aacaccagaa ttaccaccac cgataacagc aatctctttt cctttaaata
     7501 aagggccgtc acagtgtggg caataagcaa ttcctttatt tttaaactct ttttcgcctg
     7561 gtacattgat agaacgccag tgcgcacctg tcgcgatgat ggcggttttt gctttaagtg
     7621 atataccgtt agctaagcca atattaataa aatgttgctc tttatttaag gaaacggcct
     7681 gttgattcat cataatgtcg atagggtaac tacgcacatg actttctact tgctgcatca
     7741 attttggacc ttctgtgtaa gaggtaccaa tcatattttc aatccctagt gtttcaacga
     7801 cttgtccgcc gaaagaatca gcgacaagtc ctgtacggat tcctttacgt gctgcataga
     7861 tagctgcact actagctgct ggtccaccgc caatcactaa tacatcataa ggttcttttt
     7921 caaacaactc ttcttcaatc aaagggccag tgattttttc aacaatttgt tcgatcgtca
     7981 tgcgtccgct actaaattct tctccattta aaaacacagt tggcactgcc atgatttttt
     8041 tatcatcgat ttctttttgg aacatcccac cttcaatcat cgtatgagta atatttggat
     8101 ttaatacagc taatatattc aatgcctgta caacgtcggg gcaattatga caagtcaagc
     8161 taacataact gtcaaaatgc aatgtttgtg ttattttttt gattttaaga ctcaaagatt
     8221 cactgatttt cggtgcccgt ccactaattt gtaacatagc cagtaaaaaa gaggaaaatt
     8281 catgaccgag ggggatacca gcaaaaacga ttccactttc tttgccaact ggattgatag
     8341 aaaaactagg tgtgcgcatc gaatctcttt tttcccaaga gattttagga gacatttctg
     8401 ctatttcctt cgtaaattca gctagttttt gactattttc actattatcc aaactaacag
     8461 aaaaaacaat aggagattca atcagtgcta aatattgttc gagattttgt ttagtttctt
     8521 tgtctaacat atccaccacc taaatctttc caacaagatc aaaacttggt ttcaatgttt
     8581 cgccactttc tttccatttt gccggacaaa cttcacctgg gtgggtacgt acatattgag
     8641 cagcgcgaat cttatcaatc agtgcactag cgtcacgacc gattccatct gcattgattt
     8701 ccatagcttg aacgattccg tctggatcaa taataaatgt tcctcgctga gctaatcctt
     8761 gctcttcatc caaaacatca aaaatacgag aaatctgatg agacggatca ccgatcatca
     8821 cgtattcgat tttaccgata gcatctgatg tatcatgcca tgctttgtga gtaaagtggg
     8881 tatcggtaga gcaagaaaag acttcaacac ctaaagattt caatgtactg tattgttctt
     8941 gtaagtcttc taattcagta ggacaaacaa aagtaaaatc agctggatag aagcaaagaa
     9001 tactccagtt ccccataaga tctttttcac tgacttctaa aaattctcct tgatgataag
     9061 catgtgcatt aaattcttcg atttttttcc caatcaatga catagtaaaa cctccaataa
     9121 taattattta tttataatta ttgtaattta gttggagttg caaagtcaat ggatattttg
     9181 ttatttaata aagattactc aaaaacgaag gttaattata aaaatagaag aggtcgcaag
     9241 aaaatgctac tgctggaaat actcgggata acgttgagta acagtttcct gttctttcag
     9301 catcatgtgt aagtgcaatc ccgtttgaaa tcgagcttct tctggttttt tatcaactaa
     9361 agccttgagt aaatcataat gttgttccat gatcgtttcc catggaagcc cctgaatggt
     9421 cactcgtaac cagcggaaac gttctaaatg ggtattatgg tcatctaacc agttccaaac
     9481 ttcttttctt cctgcaattt cgaaacaagc ttcatgaaat aaattatctg cacggaaaaa
     9541 agcgcgttcg tcatttattt tgactgcttt ttcttgttct tctaaaatcg tttctagcac
     9601 tattcgtgtt tgtgtgtcca atttggaaca acattcaatc atgatttgct cttccaactg
     9661 ttctcggaca aagcgagcat tttctgcaga aacaagatta attttggaaa catacgtacc
     9721 actttgaggt atcgtataca cgagttcttg ctgacgtagt tgaatcaatg cttcgcgaat
     9781 gggagtccgg ccgatagcaa gatgttcctc cagtgccttt tcagatattt tccttccagg
     9841 ttctaagtca gcatagataa tttgtttacg aatcgcttga taagcttgtg attgcaagct
     9901 gcttttcatc tttattcctc caaaatcatt tctatgaacg tagtataaca gaagaggtct
     9961 gaaaaacaat ctggtatgtc acattaacta gtaaatttgt ataaatagta aaattttggt
    10021 tgacatagcg atttttattg cataaaattc acttgaatac aaaaactaat atgttagttt
    10081 aggaggggaa aaatgattga tttaaacaac tggaggaact ttatccctga attatctcaa
    10141 aaagaagtag aaatacctca ttttgaccgt gcatcattaa gagaaaatgg acaaaaaaat
    10201 cctgtctgga tccatttcgg tggcggaaat ctttatcgag gatttcatgc agaaatcgct
    10261 caacgtttag cggatctagg cgaattgtcc actggtgtca ttgtttgtga aacattcgat
    10321 gaagaaataa ttgaaagggc ttaccaagat tttgatgatt tgattcttga agtggtcatg
    10381 ccaagcagtg gagagttcgt caaacgtata ttagctgcga cagctgacag tctgtattgt
    10441 catccagatt gttctgaaca ctttgataag atgattcgac actttgaaaa tcctagttta
    10501 caattagtta cttttgctat tactgaaaaa ggttatcgat tgaaagatac aaataataaa
    10561 tatataggac cgattgcagt cgatataaaa aatggtccaa agaagccatc acatacaatg
    10621 agtattcttg ctgtgctact ttataaacgt tttttatctg gagcttggcc gattgcactt
    10681 gtctctacag ataatttttc acaaaacggt agtcaattac gtacttcttt gctaacgatc
    10741 gcaaaagaat ggcaaaagga aggttatgta caaaatacat ttatccaata cttatcgaat
    10801 gaagaaattg ttgcgtttcc atggacgatg attgatcgta tcaccccgaa tccttccttg
    10861 tcagttagca aaatgttggc aaaagagggc ttttcaaata tgtcgatttt ccaaaccgca
    10921 aaaggaaata atattgctcc atttgtcaat acagaggaca tccattattt aatcatggaa
    10981 gatcattttc ccaatggacg tccgaaattc gaaaaagctg gggtattctt gactgatcgt
    11041 gcaacggtag ataaagcaga tacgatgaag gtgacgactt gccttaatcc tttgcatacg
    11101 tctttagcta ttatgggttg tttactgggg tataggagca tagctgagga gatgaaagat
    11161 ccagatttat cgctgctagt ttctgaaatc gggtacaatg aaggcctccc agttgtggag
    11221 tctccaggta tcattgatcc aaagatcttt ctggaagaag taattactgt gcgcttttct
    11281 aatccaaata ttcctaatat gcctcagcga atcgccacgg atacctctca aaaggtggcg
    11341 attcgttatg gtgagacgat taaaaaatat atggaaatag aagttaaaga cccgcagaca
    11401 cttgtattca taccattgac tctcgctgct tggcttcgat accttattgg cattgatgat
    11461 caaggaaaac cattcatccc gagtccagac ccactattga cagaccttca gcataagctt
    11521 tccgatattc aactgggtac gcaagataca tcacatattc atgagaaaat ggctcctatt
    11581 ttgtcaaata aagaaatttt tggtgtagat ctttaccaag cagagcttgg cagaaaaata
    11641 gaagaaatgt tcatcaagat ggtagcagga ccaaatgccg tacgaaaaac attacaggat
    11701 tacttacaag aacatggagg aaatttatca tggaaatgac ttttagatgg tacggatcac
    11761 aggaagaaaa gattacctta gatgaaatca gacagattcc tgcggtcaaa gggattgtag
    11821 gcacattgat ggatatacca gtaggagagg tatggcctag ggagaggatc aaagaactaa
    11881 aagaagaaat tgagtctcgt gggttaacac taaaagtaat tgaaagcgtc aatgtgcatg
    11941 atgccattaa aattggatta ccttcccgtg agcagtatat cgaaaactat aagcagacaa
    12001 tccgaaactt ggcagaatat ggaatagaag tcatctgcta taattttatg cctgtatttg
    12061 actgggtgaa atctgacctt gactaccgct tagaagacgg ctcatccacg ttagctttta
    12121 tatctgatga tattccagat gatccaagag aaatcgtcgc gcgcatcgaa cattccagca
    12181 atgaatttgg attgccggga tgggaacctg agcgcttagc tcctatcaaa tccttgcttg
    12241 aagcctatgc atcagtagac gaagaaaaac ttcgagaaaa ttttgcctat tttctccaat
    12301 caatcatccc aacatgtgaa gaggtagggg tcaaaatggc tgtacatcca gatgatccgc
    12361 catactcgat ttttggatta gctcgaatcg taaagaatcg agaagattta gattggttgt
    12421 gtcgcatagt agacagccct tataacggaa tcactctttg tacagggagt attgcagaag
    12481 atccagaaaa tgatgtatat gggattttag cagaattttg ccaaagagat cgaatcccat
    12541 ttgcacatgt tcgaaatatt aaatttttag ctaatcgaga tttctatgag gcaccacata
    12601 aatccgaata cggttcatta gacatgtacc gcatactaaa aacaatgtat gacaacggat
    12661 ttaacggata catccgtcct gatcatggaa gaatgatctg gaatgagaca ggaagaccag
    12721 gatacggctt atatgatcga gcattaggtg ctgtttatct aaatggatta tgggaagcaa
    12781 tcgaaaaagg aaacaaaagt aaagataatt agtgaaaaaa actgtaattt tattatgtaa
    12841 ataagaagga tgttcaaatt cttgatacat aaatccggag gttgttaata agcattcgga
    12901 tttatgtatc ttatttggtc taggaagtaa cttgattttc ttttttcgat tgatccatga
    12961 gtgcttcttc ttttaattca aagatatatt ctgagatcct cataatcact tttttccagt
    13021 cttctggtgt gatgattcct tttaaaatat agattttttt gatatgagaa ggcaattgtt
    13081 tcaaagggca gtcgctgata acgatgttgc agttctccat aaaatcattt tgtattttga
    13141 tcgattcatt tgaaaaaatt tgttctatct taacgatcag atctttactg atataaaaat
    13201 cacgcgtata gttcacacca atcacaattc gcggctgttt tttgtagcga tcatacaaat
    13261 gtactaggtc ttcaaccatc cattgcacat tttccttttc taaaataact ggtaacaagt
    13321 gttgattttg ttctctgaaa tccatataaa attgttcaat ggcggtttgg acttctttca
    13381 atacgctatg ttcttgataa agcatgaaag taggaagggg cgtatttggt tgattgaagt
    13441 taagaaatcc attataaagt ttatttcgtg cgaatgccaa tgagtaaaga atcttttctt
    13501 tttcaggtat aaacaaatca aatacagtac aaagggactc caccatttct cttgaataaa
    13561 gaacatgagg aacgtttgct ttttttaaaa gtttgtattg ctccaagctt gtttcttccg
    13621 attcgatttt cgtgatagaa agtcgagcta aaatattcac taagatgcgt tgttcgcgac
    13681 aaattggcag attaggtcgc aaaatatcta cagagagatc aataaaaaag tcaatatatt
    13741 catactctgt gaattctttt tgtacgtctt tcacagaagt tgcaggaaaa ttctctttac
    13801 atatttttaa tagaaaatat aatttatcta tcataccgct atttagtcgg tcaagtactt
    13861 cttttttaaa aaaagtattc atcataaaag ccactacttg cggatcttca tcataaaatt
    13921 ctgtagcagt acttgaaaag atgttccaat aaatatctaa tgctgcatat tgaatttgta
    13981 cctcttttcc agaaaaagac tttctgccat tattagaaaa ggagatagac aaaccgtact
    14041 atgctaaaaa tgcattaatc tttttcattt tcgtatacaa ataagaggca ctatagtttg
    14101 tttgctgact gaaactgata atcgaatttg tctgacagct gaacaaagaa gtcaagatct
    14161 gataactgac tgattcgttg cagtatcttc caaatagacg ataatagcag taagccggat
    14221 caattccttc aaaattcaac tttaaaaaag gtttttgctt atcaatatgc agttctgtta
    14281 gattgagacg ctcaaagtct tgttctaatt ctttgatgta tagtaaaaaa tttgattcag
    14341 acatatggaa atgatctgct aaatattgtg tagtaataaa gaaacgatgt tcagatagga
    14401 aaagaaaaat atctaatttt gtattgcttt ttttcttcaa gataaacgtt ttaatcgctt
    14461 cttgagtttg catgaaagag gctccttttt acttaaattg ttaaaacttg aaaatattac
    14521 tgtttttttc ataaatttag acaaaaaaac agtaaacata taggtagaat ctctactatc
    14581 ttatttcttg catacaaaaa aatcaaggag tgagagagtt atgagatatt caaccatctt
    14641 aatatcaaca tttgcagcct taagcttatc gcttactata aatcagacag tccacacaga
    14701 ggagacagaa acagccaaat ggacagaaac caagtgggcc aacgtcaatc gaattacctt
    14761 tcaggatgaa ttttcatcct tcaattcagg agtcgcaaaa ttaagaaaag gacaaacagc
    14821 gaccatcaaa gcaaaaattg actattcggg agacttagca cctatactac aatcagctgt
    14881 tacatttgag aatgttgatc ccactttgtc gattggctat gacgagaaca ctttgactat
    14941 ccaacacaag caagcgatat ttaccttcac cgttacatta aatcaggact tgttaaaacc
    15001 agcgcttttt actgtcaagg tatcagatgg actaccgaac gatcatcatt taacgccgta
    15061 tagtcagcgg cagacaattg agcaagattc agccatcgac tcaggaggag atcttgttga
    15121 agaaccaaca gataaaccag aaaatgaaaa taagccagaa gtaccgccta cagaaaatcc
    15181 agacggagaa cagaaaccgg aaatagaacc tggagaagaa cccgatacag aaactcaacc
    15241 agagccagac aataaatcaa aacctgaaat aacccctgga gaaaaaccag atgtaactcc
    15301 agaaccagat acggattctg gaaaccaaac agtaccggaa acaaatccag acacagataa
    15361 tgaaactgaa aatccagaaa aaccagaggt agatccagaa gaaaaaccag atgtgactcc
    15421 agaaccagat acggacgcaa gagaccaagg aataccagaa aaaataaaca aaaaaacaat
    15481 tcaagaggat ggaaaaaaag agagtaaaaa atcaaatcta gcaattctaa aaataaatga
    15541 agagcagctg aataaaaaat cacgaatatt tgatagcgcc caatcagctg aaaccttaaa
    15601 aagtagcaag gatacaacct ttgctgtcct gaaacgaaaa ataaacagct accaaaatcc
    15661 ggagagtcac aaaacaaagt tatactgtgg agcggaatca ttctattaag tatagcaaca
    15721 atgctttctg caaaaagatt taaacaaaac agatctctct aacatttgaa taatgtctgt
    15781 gaacgcattt ttcaaataaa aagaaatatc cagaaaatga ccccaaaata ccagattgaa
    15841 atctgatatt tggggtcgtt catactttat ctttcttcat atttatttta agggatatct
    15901 gtttcttttg gaagatggtt cgggatcttt ttcactttca atacatctaa aaagaaggta
    15961 aagatcggaa tgccgacaat caagccccaa acgccaaaga accgttcgct aacaagtaag
    16021 accacaaatg tataaaaaat cggcagttct gttttgctgg acatcaactt cggattcagc
    16081 acataagact caattagatg gacaatcaaa atcgtaatca ggatataaac aacatctcgg
    16141 atgcctcctt gggaataagc tatgaaagat agaggaatac aagaaatgat aacacctgct
    16201 actgggatca gacttaaaat gaaaatcatg atcgctaaac tcagcagctg atgaaagcca
    16261 aatacagcca aggcgattgt agtgagcaac gtatttaaaa gagcgacaac aaattgagct
    16321 tctaatacca aaccaaatgt attgacaaac ttatcggcaa aatagtaaat atcttgaaag
    16381 aaccaagaaa attcactttt taaaaacaat cttgagaata caattgtttt tttcttttca
    16441 atc
//
LOCUS       DACECZ010000057        16213 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.057, whole genome shotgun sequence.
ACCESSION   DACECZ010000057 DACECZ010000000
VERSION     DACECZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.057"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            205..984
                     /locus_tag="IWO35_001647"
     CDS             205..984
                     /locus_tag="IWO35_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316706.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSYVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
     gene            1030..1974
                     /locus_tag="IWO35_001648"
     CDS             1030..1974
                     /locus_tag="IWO35_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0316707.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVAIVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1990..2769
                     /locus_tag="IWO35_001649"
     CDS             1990..2769
                     /locus_tag="IWO35_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ0316708.1"
                     /translation="MEETINLEEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            2781..3479
                     /locus_tag="IWO35_001650"
     CDS             2781..3479
                     /locus_tag="IWO35_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ0316709.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3507..4271
                     /locus_tag="IWO35_001651"
     CDS             3507..4271
                     /locus_tag="IWO35_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ0316710.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            4316..5005
                     /locus_tag="IWO35_001652"
     CDS             4316..5005
                     /locus_tag="IWO35_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0316711.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            6232..6912
                     /locus_tag="IWO35_001653"
     CDS             6232..6912
                     /locus_tag="IWO35_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0316712.1"
                     /translation="MIGIEEKKGVQKDVTIDREENLRGHRFYWIGRRAQDIFFSALAL
                     IALSPVMLITALAIVIDDPGGSPIFVQDRVGRNGKLFKFYKFRSMCVDAEAKLEALLA
                     QNEMDGPVFKIKDDPRITRVGKFIRKTSIDELPQLWNILKGDMSIIGPRPALPREVEQ
                     YGDYERQRLFVQPGLSCYWQIAPHRNELSFEEWVELDVKYINERSFITDWKIIFLTLQ
                     AVLFGYGE"
     gene            6941..8254
                     /locus_tag="IWO35_001654"
     CDS             6941..8254
                     /locus_tag="IWO35_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ0316713.1"
                     /translation="MNIILLSGGSGRRLWPLSNDIRSKQFIKLFKDENGEYESMIQRV
                     YRQITTVDPDAKVTIATSKSQASAIHNQLGDKASICVEPCRRDTFPAILLAAAYLHDE
                     LGVGDDESVAVCPVDPYVDNSYYEGVEALEKLVQQNTANLTLMGIAPTYPSEKYGYII
                     PESSNEVSTVKEFKEKPDKATAEKYLAQNAVWNAGVFAFKLGYLLEKAHAMVDFEDYR
                     DLYSKYETLTKISFDYAVVEKETSIQVMRYNGDWKDVGTWNMMAEVMADNIKGKAVMD
                     DTCVNTQIVNELNIPILAMGCKDMVIAASGDGILISDKERSGYMKPYVEKISTEVMFA
                     EKSWGTYTVMDVQEGSMTVKVSMQAGQKLSYHYHNSRDEVWTVVAGQGKAIVDGMKQI
                     LRAGDVVTIAAGCKHTIEAVTNLDIIEVQIGETISVADKYKSSLN"
     gene            8432..8644
                     /locus_tag="IWO35_001655"
     CDS             8432..8644
                     /locus_tag="IWO35_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316714.1"
                     /translation="MKDRMVKPSCGMYWKLHRGVIYAFEYDVTEEMLRNAVMDRSLGK
                     HLYKEEVHKGDLFYVSSWYWRWSISG"
     gene            9060..9827
                     /locus_tag="IWO35_001656"
     CDS             9060..9827
                     /locus_tag="IWO35_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAQ0316715.1"
                     /translation="MGIEGGYLMSRMRFMNTEIDNLTMGEVLVEVEKMIQDRRCGYVV
                     TPNVDHIVQLETGKELQDVYKNASLILTDGKPLIWISKFYKTPIKEKISGSDIFSLLC
                     KMAAEKGYTMYFLGAAEGVAAKAADNLMKRFPGLNVVGTYSPPYGFEKDEDEMFKIDS
                     QIKRANPDMLIVGLGCPKQEKFMFYNCDRLGVPVSFGLGASLDFEAGNVKRAPKWMSD
                     YGFEWLFRITQDPKRMAKRYLVDDLKIFKLALKYRKK"
     gene            9854..10855
                     /locus_tag="IWO35_001657"
     CDS             9854..10855
                     /locus_tag="IWO35_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAQ0316716.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFRENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSTELSYSRTERVTNIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALKGKYADKKKLVDKAIYLERVLCNEAGGWQDQIAAAYGGFNRI
                     NFNSDGYEVLPIIITPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAVLTDKNRDLDDFGRLLDLTWRLKKATGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQNAVRSAMSNLMYIPFEFENGGTQVIHYTPEMYIPK"
     gene            10878..12164
                     /locus_tag="IWO35_001658"
     CDS             10878..12164
                     /locus_tag="IWO35_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HAQ0316717.1"
                     /translation="MKVVIMAGGKGTRISSVASDIPKPMIKIENKPVLEHEIECLRDQ
                     GFTDIILTVSHLGDIIMNYFGDGSGISPVTGQPFDVKIEYFLEKEPLGNAGALFRLKD
                     NLTEDFLLLNADAMFDVDFNRFVAYHREKGGLVTLFTHPNSHPYDSGLIIADKDGSVD
                     RWLVKEDSRPTYYRNRVNAGLHVISPAVLDVEINTPKIDLDRQLLKPLAGTGKMFCYD
                     SPEYVKDMGTPDRYYAVCEDFKAGRVKGKNLQNKQKAVFLDRDGTINQYVGFLRHIDE
                     FELIKGTEDAVKKINAAGYLAIVITNQPVIARGEVSFEELEEIHNKMETLLGYKGAYL
                     DAVYYCPHHPHKGYEEERPELKFDCECRKPKPGMLIQAATDFNIDLSQSWMIGDGEND
                     IKAGQAAGCKTALVGESEYGQDMTIATLSEFADKIC"
     gene            12198..12851
                     /locus_tag="IWO35_001659"
     CDS             12198..12851
                     /locus_tag="IWO35_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0316718.1"
                     /translation="MIDNRLQKHIDLLIKRYPTLEVIKQDIVDGYLIMEECYLNDGKL
                     LIAGNGGSAADSEHIAGELMKSFKTPRPVSREFADKLIAVDPVKGSELAKNLEYGLMA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRPGDVFLGISTSGNSKNVINATVVARA
                     LGMKVIGLTGAKGGELARVADVCVKAPETETYMIQEYHLPIYHCWCLMLEDKFFGNR"
     gene            12884..14089
                     /locus_tag="IWO35_001660"
     CDS             12884..14089
                     /locus_tag="IWO35_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0316719.1"
                     /translation="MNVLFITTQFPYPLDNGGKIGAYNGISVLSRDYDVTVLSFTEEP
                     QYVEEGVSFFREKFNSVKFENPVIHDVHIRNNPMKLVEALAIGYCLRQPYIVSKFYDS
                     KMLQLIDEKFSNNAYWDIIFIDYLNMQSYGQYIREKYKTQFGKYILKDHNIEYDLVLQ
                     EAESSRGLKKKLLQLEYKRTRKYEEAAVNRADYCFSVCDANTEVLKKINDNSYTMLPT
                     YEMLTGVKKNIEKNTILYLGNLSWKSNMQGLVWFLESIYPNIQQRIPDVKITIVGSGP
                     VGTDFSKYSGVDYRGYVKDISHIYDDQAVLIVPLLEGSGIRIKILEAFNNEIAVVSTL
                     VGCDTIGAKDGSELFIADNEKDFATAVVRLLEDMNLNEKVRKNAKLFLKEKYSLTARQ
                     DEFSAIINS"
     gene            14219..15544
                     /locus_tag="IWO35_001661"
     CDS             14219..15544
                     /locus_tag="IWO35_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316720.1"
                     /translation="MIVAAMSYHLTGNSNVASLIAVVGVVYAIVKACNNEYLAIWVMF
                     VLGSDIWGIVPYINIGSGVLHWSDFNVIVAIFLCLRFIKVKARSRYGIVYSVMLIFIL
                     IACVQSHSIYGQDVHTTLLTARELLVVISFWPIVKIVYSERITKNEFIDLVCKFTRWT
                     MCIYWFEWILVNMGFNITFLQTGARWGTRIYIDPIFIILYFFINLYAVIYKREGYNST
                     NKSIWNCIFSLLTFVIISQGRSGILYVVITSVIMLLISLKPSKIIRFGILGIALIACL
                     VSVTQIREVVISSFVESQSSDTGNIAYRIAEQDYFENKLEGHELFGVGIPNNHDARAV
                     EYSGKTIDFESKKYNPYYLEDLGAFEIRYRFGIIAYALYIVALAISIIDAGFRRDKSV
                     AAFAGMAFMVFMALNFNLLAYITARPFGFMISLILIEISAFNDEFIYIE"
     gene            15561..>16213
                     /locus_tag="IWO35_001662"
     CDS             15561..>16213
                     /locus_tag="IWO35_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316721.1"
                     /translation="MNLDRLKKIANMISPIIKVINAPLAYFSGKSDYMEKGILWPTVT
                     TYSNNSRKVIQYTTLPKNVDNMAQMQWAGESFEDICIVLQGPVLEDGDFTLNTVKMYN
                     KYYPKVKLVVSTWIGLSEETECKLREAGADVVLCKKPEKTGAYNINLQLTSAYEGVKM
                     AQKLGCSYALKTRTDQRLYANDVLKYFQTLLNIFPSSDCKVVNQRLIFTTYGKSFRYL
                     "
BASE COUNT         5420 a         2452 c         3626 g         4715 t
ORIGIN      
        1 ttattaatgt tttatttact tttaaatcat aaattgccct agtcaagaaa acagtttgtg
       61 gtatgattat atagggaaaa cttgtttaca aatatttctg tgaatatatt agtttaatta
      121 tgttttttaa aataaaaagc tgtcattttt atgtaagtga attatatctt aatttatcta
      181 tcatcaatac ttaggagtga atctatggaa aagccattcg tcaagtcatt gactgctgtg
      241 gcggtaggtg caggtgctat tgcgatctgt ttgtttggtt accatatcaa taatcagcgg
      301 caacatcagc aacggatcaa ttatgcagaa tctgccatca ccaatcaaaa agacaccttg
      361 acttctttat caaaagaagt agacaagctg tatagtacaa aagaaaaaat ttttctaaac
      421 cctgaaataa cagaagaaac aatcacaagt ttgtcaaata agctatccta tgtcaaactt
      481 tcagcagatg attacgacat caaagaatca gaattgccta aagaagctgt tgcaatccaa
      541 gatgagaaga aagcaatcgt gacacagtta gatagtgcag aatcaaaact gaagatccag
      601 acagctgtca acgatttgtt tactaaaaat gtatcaaact ggcagcaagc tgtagacgat
      661 gtcatcatca aagaaaaggt gacaagtgca gatgtcgctc gtgtacgtga aaatatgagc
      721 ttttttaaag atagtccttg gaaaacggtt gttatgcaat atttaggatt cgctgatact
      781 cagatcgcgc aagtcacgca actagatcag ttattcgata cgatgttgaa ggatggtcaa
      841 gtgactgcca ccgctactta cgatcaatat ttgacggcgc tcagtcaaat agagcagatc
      901 cggaatgaaa aaataagcgc agcatacgca acaaaagcac aaactgttgc tcagcaaatg
      961 ggctatagca atataagtta ttgacagtta aaacgttgat tcaagcaagg ttagcaaacg
     1021 gaggaaacta tggcaagacg aagaagaaaa tcaagaggga aaaaaatatt tctgattatt
     1081 ttagcagttc ttatcgtatt agtattagca attgtaggag taggagcaaa attatatctg
     1141 gatctctcac attctattga gaaaacttac gaatcagtgg aacgaaccca aggagaacga
     1201 acgaaaccag ttgatctgga caaacaagaa tctttctctg ttctattgat ggggattgat
     1261 actggagatt taggtcgggt cgatcaagga agatcagata cgatgatggt tgccatagtc
     1321 agtcctgaag acaaacagac aacgatcgtc agcattcctc gtgatacgta tgtcgacatc
     1381 gtaggtcgtg acacaaaaga caagatcaac cacgcttacg cattcggcgg agctgccatg
     1441 gcgatggata ccgtacaaaa atatgtagac atcccaatcg atcactatgt ctctatcaat
     1501 atgaaagggc taaaggaact tgttgatgca gttggtggta tcgaagtcaa taatgacctc
     1561 actttctcac aagatggcta tgactttaca atcggtaaaa tcacattgaa cggggatcag
     1621 gcattatctt attctcgtat gcgttatgaa gatccaaatg gtgactatgg tagacaagag
     1681 cgtcaaagaa agatcgtaga aggtgtagca aaaaaagtat tgagtttaaa cggtgtcagc
     1741 aactatcaat ccgttttgaa tgctctagag tcgaatatga aaacagacat gagctttgat
     1801 gaaatgaaaa aaatcgcttt agattatcgg gaggcatttg gtacgatcaa gcaagatcag
     1861 ctgcaaggag aaggctttat gcaagataat atctcctatc aaaaagtcag tgatcaagaa
     1921 cttgatcgtg taaaaaaaga attaaaagat caaatgaatc tagagaataa ataggaaagt
     1981 ggagaaaaaa tggaagagac aatcaacttg gaagaaattt ttgaaacatt acgcaaacac
     2041 ttggtaacaa tcatcatctc aatgtttgct ggcttggcca tctcaggaat cgtaacattt
     2101 ttcatcttga caccaaaata cagttcacaa gcacaattga tcgtgacact gccacaatca
     2161 gacacagcaa atgtcaatga tgtcaatacg aacctgcaaa tgatcaatac gtataaagat
     2221 atgatcgtca gtgatttagt actaaatgaa gtcaaagatc gtttagaaac agaagacaat
     2281 gtgaaaatga ctgctggaca aatcaaagat gcgatctccg tcaatcagtc acaaaattca
     2341 caaatgttct cgatccaggc aataagcacg aatgcagtga ctgctcaaca gatcgctaac
     2401 acaacttcac aagttttcca agagaacgca aaagacgtga tgaatgtcga taaaatctcg
     2461 attatttcag gtgcagttgc caatacgaca cctgtttcgc caaataataa attgaatcta
     2521 gtgattggct tagtattagg aatgatggta ggcgtaggtc tggcattctt attcgaatta
     2581 cttgatcgga cagtgaaaga tgaaaaatat gtgacggatg cattaggatt cccaatctta
     2641 ggaacagttc cagaaatgtc agcgaaagaa ttgaacgctg gtatccgtaa aaaaggaaca
     2701 gctcctgcac gaccaaaagc tacaaacgat aaaacagccc catcacgcag aagccgctca
     2761 agagtctagg aggaagaaaa atggcacgaa cacagaaaca aaaagcagca caaactcatg
     2821 cagtcagttt gatcacatta acaaacccat cttcaccaat cgccgaacaa tatcgtacga
     2881 tccggacgaa tatccagttt gcctccgcag ctggtcagca aatcaaaacg attgtcgtga
     2941 cttcatcagg tcctggagaa ggaaaatcca ccactgcagc aaacattgca gtcgtttttg
     3001 ctaaatccgg tcaacgagtt ctactagtcg atgcagattt acgtaaaccc gtcgtctata
     3061 agacattcca gctgaacaat gccaggggat taagtacagc acttagttca tcaggaagtg
     3121 tagcagatgt aatccaacga acgccagtcg agaacttgtc gattttgcct agtggaccta
     3181 agccacccaa cccatcagaa ttgttaagtt cgccaagaat ggatcagatc ctagacgaag
     3241 caagacagtt atttgacgtg gtgatattcg atatgccacc agtagtagcc gtaacagatg
     3301 cacagatcat gtcttcaaaa acggatggca ctttattggt cgtacgtgaa aatacatcac
     3361 gtaaagaatc attgaataaa gcaaaagaac tgctagagat ggtacaagca cgagttttag
     3421 gtgttgtata caacggagca gaacatagca aagatgctgg ttattactac ggaaattaag
     3481 gaataagaag agaggagaca agagaaatga tcgatctaca ttgccatatc ctaccaggaa
     3541 tcgatgacgg ggcagaaaac ttagaagcaa gtattgcaat ggctgaaaaa gcgattcagc
     3601 aaggcatcac gcatatttta tgcacaccac accacaataa tggaaaatac agcaatgaaa
     3661 aatcacaagt catttctcta gtcgcttcgt tacaagccga attagagaaa agacagttgc
     3721 ctttgacgtt attggaagga caagaagtac gtattaccgg aacgctcatc gaagacatcc
     3781 accgagatga aattttattt acagatttag atgatacata tcttctgatc gaatttccga
     3841 cactggaagt gccgttatat gcagaatggc ttttttttga gttgttagaa ctaggaaaga
     3901 caccagtgat cgtccatcca gaacgcaacg cgcattttcg agaagaccca aatcatttgc
     3961 ttccgttcct agatatggga tgtttggccc aaattactgc gccaagttat gttggtgtat
     4021 ttggcaaaga cattcagaaa acagccaaaa caatggtcaa acataatctc gtgcagatgg
     4081 ctgcttctga tgcccatggg gtctctaagc gtacctttta cttaaaagag tgctatgagc
     4141 agattgctaa ggactttggt aaggaaaaag tagtacagat gaaacaagtc gcaaaggatc
     4201 tgatcaacgg ggatgaaatc agttatccag tctatgaaga ggtaaaaaag aagaagtttg
     4261 gtttatttta gttctttatt cgtagataaa aaggaaatga ggagagaggg aaagtatgga
     4321 aagtgaagat ttattatctc gtattagtga agtacatcct aatcaagttt ggttggataa
     4381 agaaaaagtt gaaaaacgat atatctattt attttttaaa agaatgattg atattgtagg
     4441 aagcattatt ggtttgattt tagcttctcc tttgatgttc tatgtagctt atcgaatcag
     4501 aaaagaagag ccaggaagtc cgatattttt ttgccataca cgtgttggta aaaatggaaa
     4561 aacattcaaa atgtataaat tccgttcgat gtgtttagat gctgaagaaa aattggaaga
     4621 attacttgat caaaatgaaa tagaaggtgc aatgttcaag atgaaagaag atccccgtgt
     4681 cactaaaata ggtaaaatta tccgtgccaa aagcattgat gaattgccac agctatggaa
     4741 tgttttaaaa ggcgatatga gtctaattgg tccacggcca cctttggaga gggaagtagc
     4801 tgagtacacg agatatgatc ttcaacgctt gtatgtaaaa cctggttgtt caggtttgtg
     4861 gcaagttagt gatagaaata atgttagttt ccataaaatg gttgagttgg atttagagta
     4921 tattagtcat cgaagtttaa tgtacgactt gaggttgatt ttaaaaacgt tttatgttat
     4981 tgtcaagccc aatggagcat attagttagg gatagaatga tggattgtta ataaaaaaga
     5041 ttacacatgt tcatattatc gtttcaaaag taataactgt aattatggag ggtttgaatc
     5101 acttgtagaa aatctaaaaa aagaaaggtg aatggggcaa ttcagtatca ttattcttat
     5161 ataaaagaaa atattcgaaa ttttgggatg ataccactct taaatataat gatctaaatg
     5221 ctttaatatt ggtatatccg atataggaac tgacgagcta tttagtatga tacaaaagta
     5281 tattgatagc cattaataaa ttttccaaaa aataatgaca gcacaccaat ttttataatt
     5341 tagcttgttg aatcggtatg ttcatgggac ggttcaaaaa aagaatcgaa aaaataaacg
     5401 gtaaaatttt agtgaatcct atgtaataaa atttggatag atggaaaaac agcacataag
     5461 gaggctgagt ttgttgtcat ttctgctcct acaaactatg gtagtgaatg tgttattgaa
     5521 ttagtgatgt ctgttacttc agagacattg gggataatta acttcactat acctgtaggg
     5581 tatacaaaaa atggaagaaa aaatattatc aatagatttt atttagttca gaatttttac
     5641 gtggaagtaa attattgatg ataatcttta tccaattaga attattatcg taggatgtga
     5701 tgatgatagc cgtgaagttg ttcaagtatt taccaatgtc tacaacgcag aaaattagtt
     5761 tgggtttttc ggattggaaa atttcaatgt ataaaatcat aaatttgctg taaatagaca
     5821 gtttggggta gtaatagccc aataaattgt tcgataaact catctttggg gtgagaatag
     5881 ctcaatcatt gaatttcaaa cctgcctttg gggtgggatt agaggtcaat atgtacctac
     5941 ataatacttt ttggaaaatg atggtgttga gaatgtaagt acatttcaat gtgaaattct
     6001 aatatagccc caaacgaggt caataaaaat gacctaaact cgtatttctc ccctaaacga
     6061 ggaggagata gatatgaaaa ttgaatgtgc cggcgttgga gttggctgct tttagtagga
     6121 atgaacatgg ttcgatgaga actgtttcag atagagtggt cgatgatcac aggcctttaa
     6181 gtcgtttaca gatgatgaga cttgtaaaaa tagagggacg aatggaaaaa gatgattggt
     6241 atagaagaaa agaaaggtgt tcagaaagac gtaacaattg atagggaaga gaatcttagg
     6301 ggacatagat tctattggat tggacgcaga gcacaagata tcttcttctc ggctctagct
     6361 ttgattgcat tgagccctgt gatgctaatt acagcgctcg ccattgtgat tgacgatccc
     6421 ggtggcagcc cgatttttgt gcaggataga gttggaagaa atggaaagct gttcaagttt
     6481 tataaattcc gatctatgtg tgtagatgct gaagcaaaat tagaagcctt gttggcacaa
     6541 aacgagatgg atggtccggt cttcaagatt aaagatgacc caagaatcac gagagttggt
     6601 aaatttattc gaaagacaag tatcgatgag ttacctcagc tctggaacat tcttaaaggt
     6661 gatatgagta ttattggacc cagacctgct ttaccgagag aggtggaaca atacggtgac
     6721 tacgaaaggc agcggttgtt cgtacagcct ggtcttagtt gctattggca gattgctcca
     6781 catcgtaatg aactgtcctt tgaagaatgg gtggaacttg atgtgaagta catcaatgaa
     6841 agaagcttta tcacggactg gaagattatc ttcttgactt tacaggctgt attatttgga
     6901 tatggtgaat gattatatat agtattagga gttgaaatat gtgaatatta ttttattatc
     6961 gggtggtagc ggtaggcggt tatggccgct gtccaacgat attcgtagca aacaattcat
     7021 caaactgttt aaggatgaaa atggcgaata tgaatctatg attcaaagag tttatcgcca
     7081 gattacaact gttgacccag atgcaaaggt cactattgca acaagtaagt ctcaggctag
     7141 tgcaatccat aatcagttgg gagataaggc ttcgatttgt gtagaacctt gtcgtagaga
     7201 tactttccct gcaattttgt tagctgcagc atatcttcac gatgaacttg gcgtaggtga
     7261 tgatgaaagt gttgcggttt gtcctgttga tccttatgta gataattcct attacgaagg
     7321 cgttgaagcc cttgagaagt tggtgcagca gaatacggct aatttgactt tgatgggtat
     7381 tgcacctaca tatcctagtg aaaagtacgg ttatatcatc cctgaaagtt ctaacgaagt
     7441 aagtactgtt aaggagttta aggaaaaacc tgacaaagca acggcagaga aatatttagc
     7501 tcagaatgct gtttggaatg ccggtgtatt tgcgttcaaa cttggttacc tgttggaaaa
     7561 agctcacgca atggtggatt ttgaagatta tcgtgatttg tattccaagt atgagacttt
     7621 aaccaagatc agctttgact atgctgtggt cgaaaaagaa acgagcattc aagtgatgcg
     7681 ttataacggg gattggaaag atgtcggaac ttggaacatg atggctgaag ttatggctga
     7741 taatattaag gggaaagctg taatggatga tacctgtgtt aatactcaga ttgtgaatga
     7801 gttgaatatt cctattcttg ctatgggctg caaagatatg gtaattgcag caagtggcga
     7861 tggaattctg atttctgata aagagcgcag tggctatatg aagccgtatg ttgagaaaat
     7921 cagtacggaa gtaatgtttg cagaaaaatc ctggggtaca tataccgtta tggacgttca
     7981 ggaaggctcc atgactgtta aagtttctat gcaggccgga cagaagttga gctaccacta
     8041 ccacaattct cgcgatgaag tttggactgt tgtagcagga cagggtaaag cgattgtaga
     8101 tggtatgaag cagattcttc gtgcgggtga tgtggtgact attgccgcag gatgtaaaca
     8161 tacaatcgaa gccgtgacaa atctggatat tatcgaagtt cagattggtg aaactattag
     8221 cgtcgctgac aagtataaga gttctttgaa ctaaaagttc gcgaggtagt tattatggag
     8281 atactaaaat ttcaacccgt gggtaaagat tatctttggg gtggagaacg gtttaagacg
     8341 gaatttggaa aaaattgact taacaccatt aagcttatac gtgggaatgc ttagttcatc
     8401 tagacgataa ctacgtacgg aaatatcaaa tatgaaggac agaatggtaa aaccgagttg
     8461 tggtatgtat tggaagctac accggggggt gatttatgct tttgagtacg atgtaacaga
     8521 agaaatgtta cgaaatgctg tgatggacag atcacttggg aaacatttat ataaagaaga
     8581 agtacacaag ggagatcttt tttatgtcag ttcatggtat tggcgctgga gtattagtgg
     8641 ctgaaataca ggaaagctca aatgttacct atcgagttta cgattatgat cgagtagata
     8701 agaatgataa taagtgagaa ttgtactttg aaaaagcgat tcaggtaatg aatatggaag
     8761 ccactttgaa attggagaaa agaaataagc tcattcattt ttatcctggt ggctctcgtg
     8821 aaatactgtg tagatgtaaa tactgtgagc ccgaaagaat ccaagtaaag acggggtttt
     8881 cgttctcggt aaaagaggaa tcttttcaag ttcttttatg tatatcgggg gacggacaag
     8941 ttgagtcggc aaatggtgtt atgaaactca ttagatttgt aaaaaagata ttgtatgttt
     9001 ttgccaacga atattggaag atgttaagtg ttgagcgatg cggacttact gaaaattaga
     9061 tggggataga gggaggatat ctaatgtcac gaatgagatt tatgaacaca gaaattgaca
     9121 atcttacaat gggtgaagta ttggtagagg ttgaaaaaat gattcaagat aggagatgcg
     9181 gatatgttgt aactccgaat gtggatcaca ttgttcagtt ggaaacagga aaagagttgc
     9241 aagatgttta taaaaacgca tctttaattt tgacggatgg taaaccattg atttggattt
     9301 caaagtttta taagacacca ataaaagaaa aaatatcggg atccgatata ttttctttac
     9361 tgtgcaagat ggctgcagaa aagggataca caatgtattt tttaggtgcg gcagagggcg
     9421 ttgcggctaa agcggcagat aacctgatga aaagatttcc gggattaaat gttgtaggaa
     9481 catactctcc tccttatggg ttcgaaaaag acgaagatga aatgtttaaa attgattccc
     9541 aaattaaacg agcaaatcca gatatgctta ttgtgggttt gggttgccct aaacaggaaa
     9601 aatttatgtt ttataactgt gatagattgg gagtacctgt gtcttttggt ctaggagcta
     9661 gtttggactt tgaggcaggg aatgttaaaa gagcaccaaa atggatgtca gattatggat
     9721 ttgagtggct gtttagaatt actcaggacc ctaaaagaat ggcaaaacga tatttggttg
     9781 atgatttaaa aatctttaaa ttagcactaa aatatcggaa aaaataaact atattcaatg
     9841 aggacaatga agtatgatta ttacaaaaac accttttcgc atgtcttttt ttggcggcgg
     9901 aacggatatg gaagattatt ttcgagaaaa tggcggtgca gtgctttcca ctacttttga
     9961 taagtactgt tatgtgaatg tgcgtcattt gcctagattt tttgattaca gcacagagtt
    10021 atcatattct cgtactgaga gagtaacaaa tattgaagat atcgagcatc ctgcaattcg
    10081 taatgcgatg aaaatgctag atatgcatga aattcgcctt acatatgaag cagatcttcc
    10141 tgcacgttct ggtctgggta catctagttc ttttgccgta ggtatgttga atgctttcta
    10201 tgctttgaag ggcaagtacg ctgacaagaa gaaactcgtt gataaagcaa tttatcttga
    10261 aagagtcctt tgtaatgaag caggcggatg gcaagatcag attgcagcag cttatggtgg
    10321 ctttaaccga atcaatttca actcagatgg ctatgaagta ttgcctatta ttattacgcc
    10381 tgagcgtaaa aagcagctta acaacaactt gatgatgttc tttactggat tcaccagatt
    10441 ttcctctgat gttcagaagg caaataatgt cagcggcaca gaggacaaaa gagtaagact
    10501 gaaaaaaatg tatgagcttg tggatgaagc agaagcggtt ctgactgata agaacagaga
    10561 tctagatgat ttcggcagac tgcttgatct cacttggaga ttgaaaaagg ctaccggtgg
    10621 tgcgattagc actggaagta tcgatgaact ttatgaaaag ggcatggctg ctggtgcatt
    10681 gggcggtaaa cttctcggag caggtggcgg cggtttcttg gttttttatg ttcagcctga
    10741 aaaacaaaat gctgtgcgtt cggcaatgag taatttgatg tatattccgt ttgaatttga
    10801 aaacggtgga acacaggtta ttcattacac accggaaatg tatattccaa aataaagaaa
    10861 tcagcgagga taagaatatg aaagttgtta ttatggcagg cggtaaggga actcgcatct
    10921 catcggttgc aagcgatatt ccaaagccca tgattaaaat tgaaaacaaa ccggtattgg
    10981 agcacgaaat cgaatgtctg agagaccagg gattcacaga tattattctt accgttagcc
    11041 atcttggcga tatcattatg aactattttg gcgatggttc tggaatttca ccagttactg
    11101 gtcagccctt tgacgttaaa attgaatact ttttggagaa agagccatta ggaaatgccg
    11161 gtgcattgtt tcgtttaaag gataatttga ccgaagactt cctcctgctg aatgcagatg
    11221 cgatgtttga cgttgatttc aaccgatttg tagcatatca ccgtgaaaag ggtggcttgg
    11281 tgacattgtt tactcaccct aacagtcatc catatgacag cggtctaatc attgcagata
    11341 aggatggctc tgtagacaga tggcttgtaa aggaagattc ccgtccaact tattaccgta
    11401 acagagtaaa tgcgggactc catgtgatta gtcctgctgt tcttgatgtc gagataaaca
    11461 cacctaagat tgacttggac cgtcagttat taaagccgtt ggcaggaacc ggaaaaatgt
    11521 tctgctatga tagtccagaa tacgtaaaag acatgggtac ccctgatcgt tattatgctg
    11581 tctgtgaaga ttttaaggct ggaagagtga agggcaaaaa tcttcagaat aagcagaaag
    11641 ctgttttcct tgatcgtgat ggaacgatta atcaatatgt tggctttctg cgtcatattg
    11701 atgagttcga actaatcaag ggtactgaag atgcagtgaa aaagatcaat gccgctggat
    11761 atctggctat tgtaataacg aatcagccag taattgcaag aggtgaagtt tcttttgaag
    11821 aattggaaga gatccataac aaaatggaga cacttcttgg atataagggc gcttatttgg
    11881 atgcagttta ttattgcccg catcatccac ataagggata tgaggaagag cgcccagagc
    11941 taaaattcga ttgtgaatgc agaaagccaa agcccggaat gttaattcag gcagctacgg
    12001 acttcaacat agatttgagt cagtcttgga tgattggcga tggcgagaat gatattaaag
    12061 cgggccaagc cgccggctgt aagactgcat tggttggcga gagtgagtat ggacaggata
    12121 tgaccatagc aaccctgagt gaatttgcag ataagatctg ctaagggatg ttttatcacg
    12181 aaaggagagt ataaacaatg atagacaata gattacagaa acacattgat ttgctgatta
    12241 aacgctatcc cactcttgaa gtaatcaagc aggatattgt tgacggatac ctgattatgg
    12301 aggaatgcta tttaaatgat ggcaagctgc tgatagcagg aaatggcgga tctgctgctg
    12361 attccgagca tattgcgggt gagctgatga aaagctttaa gacgcctcgc cctgttagta
    12421 gggaatttgc agataaattg attgcggttg atcctgtaaa gggttctgaa ttagctaaga
    12481 atcttgaata tggtttgatg gcgattcctc tcgttgctca tgaagcgctt tccactgcgt
    12541 atattaatga tgttgatggc ctaggtgttt tcgcacagca gttgtttgga tttggaagac
    12601 ctggcgatgt attccttggt atttcgacat ccggcaatag caaaaatgtg ataaatgcaa
    12661 ctgttgttgc tagagctctt ggtatgaaag taattggctt aactggagcc aaaggtggag
    12721 aacttgcaag ggtagcagat gtttgtgtta aagctcctga aacagaaaca tatatgattc
    12781 aggagtacca tttgccaatt taccactgtt ggtgtctaat gttggaagat aaattttttg
    12841 ggaatagata aaatatctaa gcccatattg aaaggataaa attatgaatg tactatttat
    12901 aacaactcaa tttccatatc ctctagataa tggcggaaaa attggtgcgt acaacggtat
    12961 cagtgtttta tctagagact acgatgtgac ggtattgtct ttcaccgaag aaccgcagta
    13021 tgttgaagaa ggtgtcagtt tttttcgaga gaaatttaat agtgtaaaat ttgaaaatcc
    13081 tgtgatacat gatgtacaca taagaaataa tccaatgaaa ttggtagaag ctttagcaat
    13141 agggtattgt ctacgccaac cttatatagt ttcaaagttt tatgattcaa aaatgctgca
    13201 gttaatagat gaaaaatttt ctaacaatgc atattgggat atcattttta ttgactatct
    13261 gaatatgcaa agttatggtc aatacatacg ggagaagtat aaaacacaat ttggtaagta
    13321 tatcttaaaa gaccacaaca tagaatatga tttagtccta caggaagctg aatcaagtcg
    13381 aggtttaaaa aagaagttgc ttcagttaga gtataagcgt actcgaaagt atgaagaggc
    13441 cgcagtaaat agggccgatt attgctttag tgtgtgtgat gcaaatacgg aagtactgaa
    13501 aaagattaat gacaacagtt atacgatgct tccaacttat gaaatgctta caggggtgaa
    13561 aaaaaatata gagaagaaca cgatcctata tttgggtaac ctttcttgga aatccaatat
    13621 gcaaggatta gtttggtttt tagagtcaat ttatccaaat attcaacaaa gaattccgga
    13681 tgtaaaaatc acaattgtcg gtagcgggcc agttggaaca gattttagta aatattcagg
    13741 cgtggattat cgtgggtatg taaaagatat ttcacacatt tatgacgatc aagcggtgct
    13801 aattgtacct ttgttagaag gtagtggtat aagaataaaa atattagaag cgttcaataa
    13861 tgaaattgca gttgtatcta cattagttgg gtgtgatacg attggcgcaa aagatggaag
    13921 tgagttattt atagcagata acgaaaaaga ttttgcaacg gctgtagtta gactgcttga
    13981 agacatgaat ttgaatgaaa aagtaagaaa gaatgcaaaa ttattcttaa aagaaaaata
    14041 ctccttaacg gctagacaag atgagtttag tgcgataatt aacagctaat atatatataa
    14101 gcttatctga tgatgattta taggaggaaa tatatggaga tggctgagag aaaggaaaag
    14161 aaacttggaa ataagaatat catattattt tagaaaactc ctgttatttt atgtttgtgt
    14221 gattgttgcg gctatgagtt accaccttac aggcaattcc aatgtagcgt cattaattgc
    14281 tgtagtagga gtagtttatg ctattgtaaa ggcgtgtaat aacgagtatc tggctatatg
    14341 ggttatgttt gtattaggtt ctgatatatg ggggatagtg ccatatatta acattggtag
    14401 tggtgtgttg cattggtcag attttaatgt tatagtagcg atatttctct gcttgagatt
    14461 catcaaagtt aaagctagga gtagatacgg gattgtctat agtgtaatgc ttatatttat
    14521 tttgattgca tgtgtgcaat cgcattcaat ctatggacaa gatgtacata caacgctctt
    14581 aacagctaga gaattgctag ttgtaatatc cttttggccg atagtaaaaa tagtatattc
    14641 agaaagaata actaaaaatg aattcattga tttagtgtgt aaattcacaa gatggacaat
    14701 gtgtatatat tggtttgaat ggattcttgt taatatggga ttcaatataa cctttttaca
    14761 gacaggtgca cgctggggta ctagaatcta tatagatccg atatttatca ttttatattt
    14821 ctttataaat ctatatgcgg ttatatataa aagagagggc tataatagta caaataaaag
    14881 tatatggaat tgcattttta gtttactgac atttgttata atctcgcaag gcagaagtgg
    14941 tatattatat gtagtaataa cctcggttat tatgctttta atttcgctta aaccatccaa
    15001 gattattaga tttggtatat taggaattgc cttaattgca tgtctagtgt ctgtgaccca
    15061 aattagagag gtcgttatat cttcgtttgt agagtctcag agcagtgaca caggtaatat
    15121 cgcatataga attgctgaac aagattattt tgaaaataaa ttagaaggtc atgaactatt
    15181 tggagttggt ataccaaaca atcatgatgc cagagctgta gaatattcag gcaaaacaat
    15241 agattttgag tctaaaaaat ataatcccta ttatcttgag gacttaggcg cttttgaaat
    15301 tcgttataga tttggtataa ttgcatatgc gttatatata gttgctttag ctataagtat
    15361 tatagatgcc ggatttcgaa gagacaaatc ggtcgccgca tttgcgggta tggcattcat
    15421 ggtgtttatg gcgcttaatt ttaatttgct agcgtatatt acagcaagac cgttcggatt
    15481 tatgatttct ttaattttga tagaaatatc ggcattcaat gatgaattca tatatattga
    15541 ataggtaagg gagagcacag atgaatttag atcgattaaa gaaaatagca aatatgattt
    15601 caccaatcat aaaagtgata aatgcgccgt tagcgtattt tagtgggaaa tcagattata
    15661 tggagaaggg aatattgtgg cctactgtaa caacctactc caataacagt agaaaagtga
    15721 ttcaatatac aactttacct aaaaacgtag acaacatggc tcaaatgcaa tgggctgggg
    15781 aaagttttga ggacatatgt atagttcttc aaggtccagt attagaagac ggtgatttta
    15841 cactaaatac cgttaaaatg tataataaat actatcctaa agtaaaactt gtagtgtcta
    15901 cttggatagg attatctgaa gagacagaat gcaagttgcg tgaagctggt gcagatgttg
    15961 tattatgcaa gaaacctgaa aaaacaggtg cgtacaatat taatctacaa cttacttctg
    16021 catacgaagg cgtaaaaatg gcgcaaaaac tggggtgtag ttatgcgctg aaaacgcgaa
    16081 ctgatcaaag actatatgca aatgatgtac taaagtattt ccaaacatta ttgaatattt
    16141 ttccatcttc ggactgtaaa gtagtgaatc agcgattaat atttacgact tatggaaaat
    16201 catttagata tct
//
LOCUS       DACECZ010000058        16030 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.058, whole genome shotgun sequence.
ACCESSION   DACECZ010000058 DACECZ010000000
VERSION     DACECZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.058"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..287
                     /locus_tag="IWO35_001663"
     CDS             <1..287
                     /locus_tag="IWO35_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0316722.1"
                     /translation="TSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLT
                     NKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(442..1428)
                     /locus_tag="IWO35_001664"
     CDS             complement(442..1428)
                     /locus_tag="IWO35_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0316723.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1451..2017)
                     /locus_tag="IWO35_001665"
     CDS             complement(1451..2017)
                     /locus_tag="IWO35_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ0316724.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2031..2447)
                     /locus_tag="IWO35_001666"
     CDS             complement(2031..2447)
                     /locus_tag="IWO35_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ0316725.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(2904..4838)
                     /locus_tag="IWO35_001667"
     CDS             complement(2904..4838)
                     /locus_tag="IWO35_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0316726.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5140..6075
                     /locus_tag="IWO35_001668"
     CDS             5140..6075
                     /locus_tag="IWO35_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ0316727.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6165..7268
                     /locus_tag="IWO35_001669"
     CDS             6165..7268
                     /locus_tag="IWO35_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ0316728.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7281..8234
                     /gene="menA"
                     /locus_tag="IWO35_001670"
     CDS             7281..8234
                     /gene="menA"
                     /locus_tag="IWO35_001670"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ0316729.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9048..10394)
                     /gene="gor"
                     /locus_tag="IWO35_001671"
     CDS             complement(9048..10394)
                     /gene="gor"
                     /locus_tag="IWO35_001671"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ0316730.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10528..11769)
                     /locus_tag="IWO35_001672"
     CDS             complement(10528..11769)
                     /locus_tag="IWO35_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0316731.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(11787..12710)
                     /locus_tag="IWO35_001673"
     CDS             complement(11787..12710)
                     /locus_tag="IWO35_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0316732.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(12931..15423)
                     /locus_tag="IWO35_001674"
     CDS             complement(12931..15423)
                     /locus_tag="IWO35_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ0316733.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15430..15897)
                     /locus_tag="IWO35_001675"
     CDS             complement(15430..15897)
                     /locus_tag="IWO35_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ0316734.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4701 a         3341 c         2851 g         5137 t
ORIGIN      
        1 ccacaagtga tagctcagat tcatcaactt caagcgatag tacagacacg tcgtctacca
       61 caagcgatac acgtcatgca agtgtctcct tctcaaaaac aagttcggat aacaggacga
      121 actttaactt aactaataaa acaagcaatg acaatggaaa caaatcaaaa agagcacttc
      181 ctaaaactgg aagtcaatcg aataattgga taacactcgc aggcgttata ttacttgtaa
      241 tcggtttaag aatcactttt atgagttata agaaatcgaa aaggtaaaaa aatattcaag
      301 ataaaaaaga gcaacgtcgc taacggacgt tgctctttat ttttgctttt tatcataaat
      361 acaagaacta caattcaatt tgtcaaaaaa cacgtagatt tccttctgag aaatttattt
      421 acgtatcgaa cctttcccta ttcagtcttt gcgatccaaa atcagttggg taagggaaaa
      481 gagattctct tttgttcgaa gagaggtggc aggtaatttc tttatttcct tcaaagcctt
      541 ttctgtgtaa gaggccgcta acttcctagc ttcttccacg cctcctgtat gttcaacgat
      601 tttgcgtacg gcgtcgacat cttcttctgt cattgccgct ttcttctcta aataaggtag
      661 tagctttggt ttgttttttt gcaaagagta gatcaacggt aaagaataga ccccttgacg
      721 catgtcttct agtacaggtt tgccgatttc ctggctctct tttgtataat ctaaaatatc
      781 atcgataatc tgaaaggcca tcccgataga aagtccgata tctcctgctt tttttgcgaa
      841 gcgctcgctt gttccacttt caaaagcgcc aacagaacaa cttaattgga acagttcagc
      901 tgtcttacct gaaatatttt ccaaatattc ttttacagaa agttcataat tatagcgatc
      961 atccatctgc cctaattcgc cattcaatac tttttccata ctgcgagaat tcaattggag
     1021 acttttcaag gaagtggagt aatcagacaa tagtttaaaa cagcagacaa ataaatagtc
     1081 tcctgcatag acagaaacga cattaccaaa ctgactacgg atattaggct gaccacgtcg
     1141 cagatccgct tcatcaacga tatcatcatg gatcagtgtg gccgtatgga gcatttcgat
     1201 agaagctgcc aaagcgatgg ctttgtctcg atcttgctct ggtccaaatt gtgagaacaa
     1261 tagctgatag gcaggacgca acagctttcc gccggcatgg atcgtttctt tgattgcttg
     1321 ttcgacaggt ttgtttttca ggcggacagt ttcttccatc cgttttagtg tttctgtcaa
     1381 ttctttactt aaagcaggat agttttccca cattggatgt agcttcataa aaaaactcct
     1441 tgtgtgacga ttattttttg gtaaacattg ggctccattt ttgtttggtt ccctgctcat
     1501 aatctttttg gaatcgttga agtgtctgca catgtcgtaa aagataattc gctgcgatcc
     1561 cgattgcaat ccctgataaa atccctaaaa ctgaaaggac tggcaagtac aacatgactg
     1621 tccatgattg tgcaatgaaa gaagccatca aaagctgtcc gacattatgc atgaaaccgc
     1681 cggccgcact aattccgatg atactgacac gttttggtcc taattgcttg accaataaca
     1741 ttccaaaata actaagcaaa gcaccactca tactataaag gaatgtggaa atcgttccgc
     1801 ctagcaaagt agtcaggaat agtcgtagcc aaagtagtaa gaagctgtct cgttttttca
     1861 tggtaaaaat ggcaacgatc gtgattagat tggctaagcc tagttttgca ccaggcgcaa
     1921 aagaaaaagg ataagggatc atattttcca ataaaccaat gatcactcct tgtgcaacaa
     1981 gtagcgaaat ataaatcatt ttttgtaatt tactcatggt gtgtgaaggt ctaataaatc
     2041 agactgtctt cgtcacctcc atctgttgaa cggacttcga tcactaattt atgtggcaaa
     2101 cagacgatgg tttcgccatt tttcgttgcc cagcctcgac gtacgcagat ctggtcatcg
     2161 caatctgctt cttttatccg tactcgatcg ccatctatct cgatcaagtt ataatctccg
     2221 tgttcatctt tgtaagtata tgtgttgctc tcttctccag ccatcaaagg gaatgttttg
     2281 atttctgtgc catccacacg cagtaccgct tctttttctg ctgttgcggt gttttgctga
     2341 agaccaaaaa tcatgactgg taaaaaagaa agaacagtca aaagcaaaat aataaaaaca
     2401 tcccaaggtt tgagcagact tttttcagca aattctttaa ggctcatttt tgttctcctt
     2461 tttccagatt aactcaccca attttagcat aaaaacaaga ggttgtgaca cctgtgtctt
     2521 gctttgagta ataagaacga agaatagaaa acagctttcc atgttttcta attctttggg
     2581 cttattaccg agaagcagca ggtggaacac cgtttataac aagaggttgt gacagaagtg
     2641 ggcaacttca agaaataaga aggaatttac gaaaattact tttcaagttt ttgtgaaatt
     2701 tcggcttatt tttcgaagaa gctacttctg gaacaccgtt tataacaaga ggttgtaaca
     2761 gaggttgtga cacctgtgtc ttgctcatgg aggcttatct cgggcatctt attcctttaa
     2821 ttttacataa aaaggaggct gtgacggttt gtctcagccc ctttctattg aaatcttgca
     2881 ttcaaataac gttctttatc tatttatttc ttgccgtact gggaacttgg tctaccgtac
     2941 caccaattac ctagtgtacg ttgtagccat ggaattaccc agcgatccaa cccaatcgca
     3001 cgtccggaac cgttcatcaa tgcgaaagca acaaaaatga accaaatgtt tacccagtaa
     3061 aacatgcctg ataagcagaa cgtgatcacc aatacaatcg ttgtcgcact gcatagccat
     3121 gtgaataatc cagcaatcaa tgctaatgcc aaggcaattt caaagaatac catgaatttt
     3181 tggaagaaca ttgctacatc attgtttggc atgatcactt gcatgatctt tgtgaaccag
     3241 tctggtgact tctcaatcac agccattggt tcttcaccgt atgcataact caaaccaaaa
     3301 tgtgcagctt gagtcgctgc ttctccggca cttgcccctg ctccgctggc agctgtggtt
     3361 gcatcaccag cagcagaagc accactggca acatctgtta cattgtattc ccatgtccaa
     3421 gggaacacac tgtttccagt gaaccatgat ccttcaccaa accaagtact tggtttgaac
     3481 acttcaccat tcccaatgat ttttttggaa gcttcaacga accaaactaa accatagaaa
     3541 atacgaagtg gcacactcca taagacattg ccgtaacgag aagaatgtcc tcgagtcacg
     3601 ttgcgatcat ctttgatatg gaaaaactca tgcattaaat attggaacat ataataacca
     3661 gaacgaatat caaagaagta tttcaaattc acgatatgtt tcattacgat agctaaaaat
     3721 ccgcttaaat ggatcttatc aaataggtta gctacccccc atttggcacc aatagaaacc
     3781 ataaatcctt gatagtttcc tttaaattta tgcttttctc cgcctttgat cgaagccacg
     3841 atattcgctg cggctgtgtg tcctgtttgt tcagcagctt gaacgatctg cggtgtcggt
     3901 gttcctggga attcttcata aaagactaaa tcaccaatga tatagatgtt tttatcttcg
     3961 tagccttttg cctgcatata ttcattggcg atcaaccgct gtccgcgtgc tgattctaat
     4021 ccaaagtcag cagcgtcaga agttcctttg acaccagctg tccaaatcag tgtgtgagtc
     4081 ggaatgttgc gtccgtcttt caatttgata tgatcagcag ccacttctac gattggtgca
     4141 ttcaataata attccacgtt gtgcttacgc aaatagcgtt cagcttttgc agcatcattt
     4201 cgatccaaca tattcaagat cgtcggcatt gcctctacga ccatcagtgt gatttcgttt
     4261 ggatctaatt tataatcttt ggctaggcga tttttccagt cgatcagttc cccaatcatt
     4321 tcaataccgg taaatccaga accacagaca acgaaagtca gcatggcttt acgcagttca
     4381 tcatctggtt ccaatgcagc ttttgctact gtcttctcaa tatgttcccg aatccttaca
     4441 gaatcttcaa atgaccaaag ggtgaatccg tgtgctttga caccaggtgt accaaagtca
     4501 ttcggctctc cgcccattcc aaggatcagc tgatcaaaag gataggaacc taatttggtc
     4561 ttgacgattt ttcgttcttt atcgataccg attacagtgt ctgtgacaag ttgtacattc
     4621 ttttgacgag caaacaaatg ctgcagatcg tattggattg cttctggttc tacacgtccg
     4681 ccagctactt catgcaactc ggtcatcata gtatggtacg aatgacgatc gatcagagta
     4741 attgctacat cttctttttt cagtttcttt gccaagtatt ttgtagctga aacaccggca
     4801 tatcctgctc ctacaacgac aatattttgc tttgccattg atcatccgct ccttatagac
     4861 tatctatata atgaatatat gataattctt ttagtttgat acttattaca caatctccat
     4921 atattatata gatgttctgt tcttttgtct aactcctttt ggattttttt caaattttaa
     4981 aataaagcgt tttttcacga aatttgctga aagaaaacgt ttgaaatatt acatgggtta
     5041 tttatcaaaa atagttgact tataggctaa aaagattaga atgtataatg aatcacaaag
     5101 aaaaattgtg gactagaaat ggaatggtgg ggtattaaaa tgaagaaaaa acgttttgta
     5161 actggatttg ccgtattagc attttcagct ctagtgttag gcgcttgcgg cgcagatgac
     5221 aacggaagct caaactcttc ttctgaatca tctacagcac aatcttcgac tgcaaaaagc
     5281 acaacagaat catctgcaaa agttgtagct ggcggagatt tgcaagacgg cacatataaa
     5341 ttagaagaaa aaaattattc aaatggttac cgtgcagtct ttgaaatggt tgtaaaagac
     5401 ggcaaaatca ctgaatcaaa atatgataac gtcaatgaaa acggcgaatc aaaaacaaaa
     5461 gatgcagaat acaacaaaaa catggaagca aaatctggca caaacccaga aaaatacatt
     5521 cctgagttga atgaccaatt cttaaaagca caaagcgcaa gcggtgtgga agtcgtaact
     5581 ggagctactc actcatctga aagcttccaa aactacgcac aacaattgat ccaagctgct
     5641 caagctggta acacagatac aatcgaaatc gacaatggtg ctgatttgaa agacggcact
     5701 tacaagcttg aagaaaagaa ctactctaac ggctaccgtg tgcaattcga aatgacagtt
     5761 gctggcggca aagtaactga atcaaacttt gattatatcg acaaagacgg taaatcaaaa
     5821 caagatgata cagaatataa tgaaaatatg aaagcaaaaa gcggtacaga accaaaaact
     5881 tacatcccta ctctgaacga cgaattcgtc aaagcaatgg gcgaagaaga tggctcacct
     5941 gctaatgtag aagttgttac aggtgctaca cacagctcac attcattcat catgtacgca
     6001 caacaattag tgaatgcagc tgaaaaaggc gacactcaaa caatcgaagt agataatatc
     6061 gtaacagaaa aataatcaca taatcatact aataaaagtt ggagggtggg acgatagcca
     6121 gattaggcag tcgtctcacc tttcaaaatg aagagaggaa aaagatgcgg aaaaaactac
     6181 tattttcctt aatcacactg ggcatctctt ttatggtgct aactggctgc agcaactcag
     6241 caaataaaac aaccactgaa agttcagcag ctaccaagat attgaaagat ccatattctg
     6301 atgaacagtt cttgttaggg acttacgttc gtatccgtgt gtatgatgaa ggcaaagaat
     6361 cagcattgaa acctgccttc gatcgagtaa aagaacttgg tgataaaatc acgatcaatc
     6421 aaaaaggttc tgagatcgat gaagtgaatg agcaagctgg gatcaaacca gtaaaagttt
     6481 ctgatgacgt ctacacgtta gtcaagcggg cctacgaata tagccaagat tctcaaggcg
     6541 gattcgatat ggcgattggt gcaatcacac aattatggcg aatcggtttt gatgacgcca
     6601 gaaaaccttc ccaagaagaa atcgatcagg cattgaaatt agtggattat cataagatcg
     6661 aactaaatga caaagaaaaa actgtttatc taaaagaaaa aggaatgatc atcgatttag
     6721 gtgcaatcgc caaaggttac attacggacg aagtagtgaa agtcttgaaa aaacaaggag
     6781 tgaccactgc catcgttgac ttgggcggta atgtctatgt cttaggccac agcccaagag
     6841 gcgaaaacca agactggaca gtcggcatcc aagatccgaa catggctcgc ggatctgttt
     6901 taggttctat taaagaacgg aacaaaacat tagttacctc tggaatttac gagcgttatt
     6961 tagaagtaga tggcaaaaaa taccatcatt tgtttgatcc aaaaacgggt tatccgttcg
     7021 acaatgatat cgcaagtgtc acgatcataa cagataaatc aattgatggt gacgggttgt
     7081 ctacagcagt cttttcaatg ggtgtgaaaa aaggactgga atacgtcgaa agcgaattga
     7141 ataacgggac ggaagcgatc ttcgtgacaa aagacgataa agtatatgtg accgatccaa
     7201 tcaaagacac cttcaaatta tcggaggact cccattacac gatgggtgac cgttctgaac
     7261 taaaataagg agtacgaacc atgacactaa aaatcttttt agaagtagtt gaaatacgaa
     7321 caaaggtggc tagtgtattt ccttttgcgg taggggtatt attctctatc gctttttttc
     7381 atgagataca ttggctaaac acttttttat tttttattgg aatgctagtt tttgatttag
     7441 cgactaccgc tatcaataat tacatggatt atgaaaaggc tcattctgaa gtatataagt
     7501 atgaagaaaa tgtcattggc cgcagaaatc tatctcctgt attgattcga aacatgattt
     7561 tagctatgat tgcatttgtt tttgttatcg gcgttattct aacgatcaat acgggttggc
     7621 ttttactttt gatgggattt atctgttgtt ttattggagt attctataca ttcggaccga
     7681 ttcctttatc tcgaatgcct ttaggggaaa ttttcagtgg ggtgactatg ggccttggaa
     7741 tttttgccat ggtcgtttat gtcaatacat tcggttcaga tgtctatgat ctgacagtca
     7801 acttttcatc tggacaattt gctttggtag gaaatctttg ggctatagca gcaatcgtat
     7861 tagcttcctt acccttggtg tttacgattg ccaatatcat gttggctaat aatttgcggg
     7921 atcttgaccg tgatattgaa aatcatcgct atacactagt tttttatatc ggacgtcctg
     7981 cgggaacagt cttattccag ctattgatgt atgcttgcta tttcgtgatt ttaatggggt
     8041 tgttgagcgg tatctatgaa tggccgatct tggtcacctt cttgaccttg ccaattgtga
     8101 agaagcattt ggatcaattc aagcaaacgt taccccagcc tatcagtttc agctattcca
     8161 tcaaaaatat ggtcttattc aatgccagtt atgtattggg gctattacta acaatcatta
     8221 tgcaagccat ttaacagcat gaagaggttg tgaaaaaact gccctgcatc aagtaataag
     8281 aacagccaaa taaaaatagc tccttatatt tttcgtttgg ttgggcttat taccgcagat
     8341 gcaagctttt gaacaccgtt tatcaggagg ttgtgaaaaa gtcgcttagt ttcgagtatt
     8401 aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttggct taataccgag
     8461 aaactggctt ttgaacaccg tttatcagag gttgtaaaat cagtgttttg tcctgagtat
     8521 tagagaaaca aatggaaaaa tagttcctca tattttgccg ttttttgagc taataccgaa
     8581 ggacagctga ttgaacacta tttgttcgga aaaagtgact aactatgaca caattttttt
     8641 cacagtccct tttttcactt gatttgattc gtaccagcga acttgacgag aactttcttg
     8701 gtttggtaac tatatggtat tcttttaaca tcctcgtttg cttcttctaa tttatttata
     8761 caaagtcaca cttttttaaa catctgttct tcgatacatt tctatttctt ttaaacatcg
     8821 ataccaatat tttgaactta agagtgcata ccaaatggat tcatttactc ttcgtcagtt
     8881 acgaagataa tcaaaatctc caacttccct acttatttcg ttttgagaat tatcgaagca
     8941 atcttacata aataactaaa aaacaaaaaa ccagaaaatc agtatactga ttttctggtt
     9001 ttacatggtt tgttcaataa aaagctgttg gaactgttaa tattaaatta tcgcatcgtt
     9061 acgaattctt ccgctcctgt tggatggatg gctactgtat tgtcaaagtc ttctttagtt
     9121 gcacccatct tgatagccac ggcaaaacct tgcagcatct catcgacacc aacaccaata
     9181 gcatgtaagc cgacgattct ttcttctttt ccgacacaga tcaatttcat ttcgcatttt
     9241 tggcgataat catttaatgc aaagtacatt ggggtaaagc gtgaacgata aactttcaat
     9301 tcattttcgc catatttttc caatgcttct tcttctgtca aaccaattgt tgcaattggt
     9361 ggatgcgtaa agacgactgt cggaacaagt ttatagtcaa gataagaatc tttctttcca
     9421 ttaaataaac gttctgataa acgtctacca gctgcgattg caacaggagt cagatctaat
     9481 ttaccgatca catcaccaac agcaaaaata tgttcatccg ttgtattttg gtatttatct
     9541 actttgataa accctttttt gtccaattcc acatttgtat tttccagacc taatttttcg
     9601 gtattcggta cacgtccacc ggcaaaaagt acacaatcac cagttaaggt ttcaccattt
     9661 tcaaatagca ccgtatattc gtcattgttt ttagtaattt ctttagcaat atgatgtgcg
     9721 taagttttta ttccagcttc ttggtacatt tggatcaaat tgtccgaaag cattttatca
     9781 aacgttctaa gtggtcgttc ttttcgatat gcccacatga cttcactacc taaaccacga
     9841 aatacacctg aaagttcagc cgcaatgtat ccagcaccta aaacgattgc tctcttaggt
     9901 aactctgtta attcaaaaaa tccatttgaa tcaattgcgt attctccacc aggaatggtc
     9961 attttacttg cacggcctcc agtagcgatc aagattttag gagcacgata ggtttcaccg
    10021 tttacttcga tcgtttgact atcgataaaa gtagcgtaac cttttatagc ctcgacatga
    10081 ttgctatcta atcctctttg ataagcaccg tgtagaaaat caatatattt ttctcgtctt
    10141 tctactagac cggcgaaatc aacatttttt aaatctgctt ggataccgta acttgaggca
    10201 tctcgttcaa ttgtttccaa aatcgtactt gcttgccaca taactttttt aggtacacaa
    10261 ccgacgttga cacatgttcc gcccaattca ttcgcttcaa tcagcaaaac ttttgcgcca
    10321 tgcatccctg ctctgtttgc tgaagcaata ccgccagagc cgccaccaat tacgatgtaa
    10381 tcatatattt gcattttgtt cctccttatt gaagagatca atcaaaacct ttatttaacg
    10441 atcctttttc tacagaaaac aagtgtaaca cttttcttga attttgtatg cgaagatgct
    10501 taaaacaggg atatatcagc tttttcctta tttacgtttt cggatcatat ctccaggttt
    10561 caccggctgt gcgactggca tagttaaaat catcattgga tttggtgctc ggtcaatgga
    10621 ttcgccttct tcattatgca taacttcaac ggtttgactg aaatgactga atcctggacc
    10681 ataaaattcg atttcatctc ctacagaaaa caagtttctc tgacgtatcg tagcaatctg
    10741 tgttgcctca tcataagata atacttcacc gacaaattta tatactggga ttttacgacg
    10801 ttcaccgaat aattgttcgt tttcagttgg tgtattgtag taaaaacctg tagctaattc
    10861 tcgttgtgct actttccata actcgtcgat ccattcttgc ttgcagacat agttttcagg
    10921 atcttccata taactgtcta cagccgcttt atagacattt gacaccgtag atacataatg
    10981 gatcgatttc attcttcctt cgatcttgaa gctgtctacg ccgttttcaa tcaattctgg
    11041 aatatgttcg atcattgaca tatccactgc actcatcgag aattcttctt ccacttcgcc
    11101 tgtatcagtt aaactgtttc gttcttgacc aaaaggcata tcgaacaatc catacttcca
    11161 gcgacaagat tgagaacatc cgccacggtt tgcatcccgc atcgacatat ggttggacaa
    11221 tgtgcatcga ccagaataag agatacacat ggcaccatgg ataaatgctt cgatttcggt
    11281 atccgtattt ttacgaatct ctgccacttc ctccattgaa acttcacgag ctaaaacgac
    11341 acgttccaac ccttcttctt tccagaattc caatgtctca aaattagttg ctgaagcttg
    11401 agtggataaa tggatcggca gacctggggc ttctgtcgca caaatttcga tcaaagctgg
    11461 atcagaaaca atcactgcag aaataccgac atcacgcaaa ctgcggaaaa attcccctgc
    11521 tccatcttca tttccttcat gggtgaccat attagcagca acgtatacct tcgcatcatg
    11581 ttcttttgca aaagcaactc cttcagccat ttcttcataa gtaaaattac ctgcacggct
    11641 tcttaaacca tatgcattcc cgccaatata tactgcatct gctccataat ggatcgcggt
    11701 ttttaacttt tccagtgttc ctgctggcgc taggatttct ggtcgtttca attttctttt
    11761 tactgtcatt tctttcatca tactccttat ttgatagcag aaggatcaat ataaaagaat
    11821 cctgtatcta attcacggtt ttcaggatgc aatttactga tttcgctagt cattttttcc
    11881 gctttttctt gatcccagct accttctaaa atttgttttt ttgcttcggc gaatagtcga
    11941 gtgatgttta cgaatttttc gcctggagta aataatccgt ccaatttcca tgtacagtaa
    12001 tgatgctgat aaagttcatt caattcattc gagagattca gatcattgct ggcaaaaata
    12061 tgtgtgccat gactatcttc ataaatagaa taatgtgtct cttctttttt aggttccgag
    12121 ataaaaagac ctctttcacg atctttttgt tcgtcttgtt tcgtatagtt atagtaattc
    12181 tgtagcagtg gacgtttgga ttgatggatg catgtagcac catacacaag ggtttctaca
    12241 ggaatatcga gtttttcttc catcgccttc atctcttcaa aaggaacttc acgagcaagt
    12301 actgctccaa ctgcgccttt tttcgcccag aaattgattt gacgtgcgct cgttactaat
    12361 gtttcagcat cataaatata cggtatacgc aattcaggat tttttttcat cgtataaata
    12421 atacctgtat caccaactgt gatcacatct acgtttattt cttttaaaaa agccaaatat
    12481 tcaggtacat ttttcatttt ttcaggatga atgatcccat ttacagcaat attcgctttt
    12541 ttaccatact tgtgtgctaa ttctaccaat tgtcgctgtt cttctcgtgt aaatgaagca
    12601 ggcagacgca atccaaattg ctcttcaccg aaatatagtg tatctgcacc tgcagcaagc
    12661 aactgttctg cttgttcaat agtttctact gtggttatga gttcgatcat aattttctcc
    12721 tcctacaact tcataatagt agcatagctc taaaaacttt ccaatacttc ttctatttaa
    12781 agtcggattt ttgaaaatta tttcacaaaa aatcaaaata aagttcgata tattctgtcc
    12841 aaatataaaa agagattgtg acaaaaatct tgtcacaacc tcttaatcaa gtcttactat
    12901 tataaaaatt cagctacaca ggccaagtaa ctatagggaa cctactgttt cctttttagg
    12961 tccgcgaaca ttcaatgtga tcttaccttt tgaagcacct actgttacgt ggtcacctaa
    13021 acgaatttct ccgccaagca aagcctcgct caatcgatct tcaatttctt tttgaagtgc
    13081 gcgacggatt ggacgtgcac catattcagg atcaaatcca gctttcccga tcacatcgat
    13141 tgcagatggt gtgattttca cttgaatatc ttgttctttc aatcgtttga taatcgcttt
    13201 gctcatgatt tggacgattg tatggatttc atctttactc aatgaatgga agacgacagt
    13261 ctcatcgatt cgattcaaga attcaggtcg gaaagctttc tttaactctt ctaagatacg
    13321 tttttgcatg gctttgtgat ctttagtaac atcttggaca ttgaatccga catttttttc
    13381 ttctcgaatt tgtgtcgctc cgatatttga agtcataatc atgattgtat ttctaaagtc
    13441 cacttttcta cctttagaat ctgttaaatg accatcatcc aatacttgca gaagtaaatt
    13501 gaaaacgtct ggatgtgctt tctccacttc atctaagaga atcactgaat atggtttttg
    13561 acgaattttt tctgtcaact gtccaccttc atcataccct acgtatcctg gaggagaccc
    13621 gattaatcgg cttgtactgt atttttccat aaattctgac atatcaacac ggatcagggc
    13681 atcttcactt ccaaacatga cttctgaaag tgctttggcc aattctgttt ttccgacacc
    13741 agttggtcca aggaacatga atgaaccaat tggacgatca ggatctttca atccgcttcg
    13801 tgcacggcgg atcgcacgtg aaactgcttt gacagcctca tcttgaccaa cgacacgttc
    13861 atgcagaagt ccttccagtt ctaacaagcg ttcgctttct tttttctcca actgctgtag
    13921 agggacacca gtccactccg atacaactgt agcaacatct tcttctgtta cacgatcaga
    13981 ataaccttgc tcctgttttg cttcaattgc agaaactttt tgcagctttt tacgtagtgc
    14041 tttttcttgt ctgcgtaatt gagcagcatt ttcaaagtct tgtttttgga tcgctgcttc
    14101 cttttcttgt actagttcat cgatttctag tttgatcaca gttgattttg ttaagtgatc
    14161 ggtttgatcc aaacggactt tcgctgctga ttcatcgatc aaatcaattg ctttatcggg
    14221 tagttgacga gaagtgatat aacgaacaga aagattgact gctgctcgta cagcttcatc
    14281 tgtgatctcc actccatggt gctcttcata tcttgtacgt aatccttgaa gaatcacttc
    14341 tgcttcttca ggagttggct catctacttg aatgcgagca aaacgtcgct ctaatgcaga
    14401 atctttttcg atatattttt gatattcatc tagggtcgtt gctccgatcg tttgtaattc
    14461 tcctcgagct aacgccggtt tcaagatatt cgaagcatca attgctcctt ctgctccacc
    14521 tgcaccaatt aaggtatgca attcatcaat gaataagatt acttgaccat cattatagat
    14581 ttcgtcgatg acctttttca ttcgatcttc aaattcaccg cgatatttcg taccagcaac
    14641 caaagatccc atatcaagca tcatcaggcg cttttcttgc atgtcttctg gcacttcacc
    14701 taaaatgatt ttttgagcta acccttcagc aatggctgtt ttgcctacac caggttcacc
    14761 caccagtaca gggttattct ttgtccgtcg gctaagaatc tggataagcc gtttcacttc
    14821 tctcgaacga ccaacaactg ggtccagctt gttttctttt gctaatttcg tcaaatcacg
    14881 tgctaatgaa tctagcgtag gtgttccttg cggtgtttgc cgtttatctt gtacatttac
    14941 atttctacga cgattcattc cttttccagc agaatcacca gctagcccca ttttttgttt
    15001 cagaacttga cgggcttttg aaaggctgat acctaagttg atcatgattc gagtagccat
    15061 gatactttca tcttttaata agctcatcaa aatatgttct gttccaactt tttgagaacc
    15121 agttttcttc acttcgctgc tagctaaagc aaaaacttgt ttcattcttg gtgaatatgg
    15181 aagaaagact cctttagggt aagaacggat accaccataa ccaatcagat gttcaatttc
    15241 ttcataaatt tccgtttcat tcaaatccat ttcacgtaat acttttccag caattccatt
    15301 ttgctcgacc actaaggcta gtaaaacatg ttctgaacta actgattgat gtttcaaccg
    15361 ttttgcttct tcttgtgcaa tttctaatac tgcttgggct ctttcagtaa aaagttcttc
    15421 catctaatct cacttcccat cttcataact taaacgttcc agaaatgctc ggagaatccg
    15481 agcacgtgta tgatcttcat attcattgaa attcaatgtg ttttttgcaa ttgcactcaa
    15541 catcaagttt ccttcttttt ttgtaattat ttggtcttca taaagttttt gaataatagc
    15601 aaaagcattt ttctcactga ttgtatcatc aaataactga tttatctgct caagtaattg
    15661 ttttttatct gaaatcctta cttttgcaat tcgtatataa ccaccaccac cacgtttgct
    15721 ttctactaga taaccacgtt ggatggtaaa acgggtatta atcacatagt tgatttgtga
    15781 aggaacacag ttaaagaggt ctgccatttc aatccttcga atctcgatca tttcactatt
    15841 ttccagaatt ttctttatat atgattcaat taaatccgag gtattctgat gtgccatctt
    15901 attcattccc tttgactaat attgacctta attataacga atttgataat agaaagaaag
    15961 aagaacgctt tttgttaaat aaatccttta gaatcaacaa aaaaaaatac ggaatatact
    16021 attccgtatc
//
LOCUS       DACECZ010000059        15908 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.059, whole genome shotgun sequence.
ACCESSION   DACECZ010000059 DACECZ010000000
VERSION     DACECZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.059"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            292..1431
                     /locus_tag="IWO35_001676"
     CDS             292..1431
                     /locus_tag="IWO35_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ0316735.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="IWO35_001677"
     CDS             complement(1498..2073)
                     /locus_tag="IWO35_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0316736.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="IWO35_001678"
     CDS             complement(2078..3052)
                     /locus_tag="IWO35_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ0316737.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="IWO35_001679"
     CDS             3335..4303
                     /locus_tag="IWO35_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0316738.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="IWO35_001680"
     CDS             4416..4694
                     /locus_tag="IWO35_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0316739.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="IWO35_001681"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="IWO35_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ0316740.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5160..5906
                     /locus_tag="IWO35_001682"
     CDS             5160..5906
                     /locus_tag="IWO35_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0316741.1"
                     /translation="MKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSLAEH
                     QLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="IWO35_001683"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="IWO35_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ0316742.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="IWO35_001684"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="IWO35_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ0316743.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="IWO35_001685"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="IWO35_001685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ0316744.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="IWO35_001686"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="IWO35_001686"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ0316745.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="IWO35_001687"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="IWO35_001687"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ0316746.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="IWO35_001688"
     CDS             10643..11185
                     /locus_tag="IWO35_001688"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ0316747.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="IWO35_001689"
     CDS             11208..12764
                     /locus_tag="IWO35_001689"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ0316748.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="IWO35_001690"
     CDS             12778..13680
                     /locus_tag="IWO35_001690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ0316749.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="IWO35_001691"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="IWO35_001691"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ0316750.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="IWO35_001692"
     CDS             15123..15545
                     /locus_tag="IWO35_001692"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ0316751.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         5259 a         2868 c         3436 g         4345 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaatgg ctcatacgga atggcgcaaa
    11401 acttagaatc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggaaagatca tggaagttcc agttggagag gccttgatcg
    11521 gacgggtagt taatccgcta ggtcagccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagca atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcgccgttgc tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga gttcatgtac aacggtaaac atgtcttgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca gcaaaattaa
    12121 gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa atcttcttag
    12241 aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta tctgtatctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgcctag
    12361 acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac ttggatgcag
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaattgc
    12481 atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca catggattct
    12541 tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaagta
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg ttaagaaaac tggttatatc gtcatcactg cagatggcgg tttagtcggt
    13081 ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg ttccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg ctcgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgta
//
LOCUS       DACECZ010000060        15833 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.060, whole genome shotgun sequence.
ACCESSION   DACECZ010000060 DACECZ010000000
VERSION     DACECZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.060"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(105..629)
                     /locus_tag="IWO35_001693"
     CDS             complement(105..629)
                     /locus_tag="IWO35_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0316752.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IWO35_001694"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IWO35_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ0316753.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IWO35_001695"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IWO35_001695"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ0316754.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="IWO35_001696"
     CDS             complement(2351..3031)
                     /locus_tag="IWO35_001696"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0316755.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="IWO35_001697"
     CDS             3157..4026
                     /locus_tag="IWO35_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0316756.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="IWO35_001698"
     CDS             4052..4567
                     /locus_tag="IWO35_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316757.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="IWO35_001699"
     CDS             complement(4614..5177)
                     /locus_tag="IWO35_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0316758.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="IWO35_001700"
     CDS             complement(5232..5411)
                     /locus_tag="IWO35_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316759.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="IWO35_001701"
     CDS             complement(5488..5997)
                     /locus_tag="IWO35_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316760.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="IWO35_001702"
     CDS             complement(6093..6473)
                     /locus_tag="IWO35_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0316761.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="IWO35_001703"
     CDS             6803..7552
                     /locus_tag="IWO35_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ0316762.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="IWO35_001704"
     CDS             complement(7736..9013)
                     /locus_tag="IWO35_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316763.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="IWO35_001705"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="IWO35_001705"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ0316764.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="IWO35_001706"
     CDS             complement(9935..10879)
                     /locus_tag="IWO35_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ0316765.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="IWO35_001707"
     CDS             complement(10881..11567)
                     /locus_tag="IWO35_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ0316766.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12041..12361
                     /locus_tag="IWO35_001708"
     CDS             12041..12361
                     /locus_tag="IWO35_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ0316767.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12515..13420
                     /locus_tag="IWO35_001709"
     CDS             12515..13420
                     /locus_tag="IWO35_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0316768.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13417..14190
                     /locus_tag="IWO35_001710"
     CDS             13417..14190
                     /locus_tag="IWO35_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0316769.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14371..15795)
                     /locus_tag="IWO35_001711"
     CDS             complement(14371..15795)
                     /locus_tag="IWO35_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0316770.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
BASE COUNT         4643 a         3295 c         2811 g         5084 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct ttttttaatt tattgcccga taatatatag aaataaggtc
    11761 tagttactgt tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag
    11821 cattcatctt cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa
    11881 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg
    11941 ccaccgggta ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct
    12001 ctttttgtgt atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat
    12061 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
    12121 taaaatcaat tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc
    12181 aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc
    12241 agttggttcg gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
    12301 gttctttgtg gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta
    12361 gacacttgga ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt
    12421 ttatgaaaaa ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat
    12481 gcttgtacta tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt
    12541 atccaagaac tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg
    12601 caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat
    12661 tttaggactg atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc
    12721 ctctagtcat aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc
    12781 attcttaaca ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat
    12841 cgacactatc gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta
    12901 ctcgttggga atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat
    12961 cgttatttta gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa
    13021 aatcattacc gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag
    13081 cgagcttgaa aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca
    13141 atgtacaatg gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa
    13201 tgatctagct gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa
    13261 aatcaagctt ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc
    13321 acaaaatatc catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt
    13381 ggaaattctg gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa
    13441 ttatttaaat tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg
    13501 ctcataggaa cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg
    13561 actgctcagc tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc
    13621 acgatcattt cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat
    13681 tctcgaggag aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta
    13741 taccttttag ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc
    13801 ttcacggaga aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg
    13861 ggtagttata tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc
    13921 aatagttccg gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata
    13981 tctggtatcc ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt
    14041 atgttgaatc tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt
    14101 tctactaatc aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga
    14161 tatctcgtat ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag
    14221 acgttcagcc ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc
    14281 tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc
    14341 acaacctctc tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa
    14401 aaccattgca atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat
    14461 atgttgtaca ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa
    14521 gaaaagaaca agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg
    14581 aaaggcactt gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag
    14641 tttcatttgg tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt
    14701 gtaagcaaag ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca
    14761 taacgtcgtc aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc
    14821 tttaggcaaa agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca
    14881 tgagagccaa acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca
    14941 tttaccaacc atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag
    15001 ctgattcatc gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa
    15061 gccgaatatc cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc
    15121 tacgacagag gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa
    15181 tacttgactg gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa
    15241 gttatcaatg aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa
    15301 tggcactaat ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg
    15361 attcactagc catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt
    15421 ttgcccacct tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa
    15481 agctacgttg cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa
    15541 acggccaaaa ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt
    15601 attgatatta ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact
    15661 gaaaatagct gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc
    15721 tccaatcgct gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg
    15781 ttgctctgta tccatgtaag catcctccaa aataaaaccg cttttatcaa aaa
//
LOCUS       DACECZ010000061        15303 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.061, whole genome shotgun sequence.
ACCESSION   DACECZ010000061 DACECZ010000000
VERSION     DACECZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.061"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(22..252)
                     /locus_tag="IWO35_001712"
     CDS             complement(22..252)
                     /locus_tag="IWO35_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316771.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(688..1254)
                     /locus_tag="IWO35_001713"
     CDS             complement(688..1254)
                     /locus_tag="IWO35_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0316772.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1254..2210)
                     /locus_tag="IWO35_001714"
     CDS             complement(1254..2210)
                     /locus_tag="IWO35_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ0316773.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2210..2866)
                     /locus_tag="IWO35_001715"
     CDS             complement(2210..2866)
                     /locus_tag="IWO35_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0316774.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3236..5254
                     /locus_tag="IWO35_001716"
     CDS             3236..5254
                     /locus_tag="IWO35_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0316775.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5681..8095
                     /locus_tag="IWO35_001717"
     CDS             5681..8095
                     /locus_tag="IWO35_001717"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ0316776.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8443..9996
                     /locus_tag="IWO35_001718"
     CDS             8443..9996
                     /locus_tag="IWO35_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0316777.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10377..12161)
                     /locus_tag="IWO35_001719"
     CDS             complement(10377..12161)
                     /locus_tag="IWO35_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0316778.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12176..13897)
                     /locus_tag="IWO35_001720"
     CDS             complement(12176..13897)
                     /locus_tag="IWO35_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0316779.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13997..14758
                     /locus_tag="IWO35_001721"
     CDS             13997..14758
                     /locus_tag="IWO35_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0316780.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4982 a         2799 c         3001 g         4521 t
ORIGIN      
        1 tctcattaaa aagcataacc attactccga tctgttttta aaattagttt ctacctctaa
       61 tatatatcga gtttttattt tgccttcaga gaatactgtt tctttttttg cagttacagg
      121 ttgtttattt tcggaaaaag ctaatatagc taagattgaa acatccatct ggaatttatc
      181 ttttttactg ctttgatcat aaaagtctgc atattcatca ctgatatttt ttctaataaa
      241 ttcttccatc ataaaaatca cctcaaaacg attatacgaa cttacgttct tgttgtaaag
      301 cgatatttga ggggcaaaaa aggggcataa tttgactgat attaagttag ctattgatat
      361 atgaatgaca tacaaaaata taaaatcttt taaaaatgct tatatatcaa tgtttttgcc
      421 attcttttat atatctgtta tatgtcaaaa aaatgtgttc aatttcaatt tgtatttgaa
      481 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
      541 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
      601 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
      661 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
      721 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
      781 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
      841 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
      901 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
      961 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
     1021 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
     1081 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
     1141 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
     1201 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
     1261 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
     1321 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
     1381 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
     1441 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
     1501 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
     1561 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
     1621 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
     1681 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
     1741 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
     1801 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
     1861 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
     1921 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
     1981 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
     2041 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
     2101 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
     2161 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
     2221 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
     2281 acagccaaga aagactgagt aagtagccgc cgatgatatc agtaggaaaa tgaacgccca
     2341 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
     2401 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
     2461 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
     2521 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
     2581 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
     2641 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
     2701 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
     2761 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
     2821 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
     2881 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
     2941 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
     3001 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
     3061 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
     3121 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
     3181 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
     3241 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
     3301 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
     3361 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
     3421 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
     3481 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
     3541 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
     3601 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
     3661 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
     3721 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
     3781 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
     3841 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
     3901 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
     3961 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
     4021 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
     4081 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
     4141 ggaaaatggc tcttcgacga ataattccgg taacaatacc aactcttcag gaaatgctgg
     4201 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
     4261 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
     4321 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
     4381 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
     4441 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
     4501 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
     4561 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
     4621 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
     4681 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
     4741 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
     4801 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
     4861 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
     4921 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
     4981 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
     5041 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
     5101 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
     5161 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
     5221 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
     5281 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
     5341 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
     5401 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
     5461 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
     5521 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
     5581 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
     5641 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
     5701 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
     5761 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
     5821 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
     5881 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
     5941 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
     6001 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
     6061 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
     6121 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
     6181 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
     6241 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
     6301 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
     6361 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
     6421 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
     6481 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
     6541 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
     6601 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
     6661 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
     6721 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
     6781 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
     6841 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
     6901 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
     6961 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
     7021 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
     7081 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
     7141 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
     7201 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
     7261 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
     7321 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
     7381 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
     7441 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
     7501 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
     7561 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
     7621 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
     7681 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
     7741 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
     7801 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
     7861 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
     7921 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
     7981 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
     8041 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
     8101 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
     8161 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
     8221 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
     8281 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
     8341 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
     8401 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
     8461 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
     8521 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
     8581 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
     8641 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
     8701 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
     8761 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
     8821 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
     8881 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
     8941 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
     9001 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
     9061 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
     9121 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
     9181 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
     9241 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
     9301 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
     9361 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
     9421 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
     9481 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
     9541 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
     9601 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
     9661 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
     9721 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
     9781 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
     9841 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
     9901 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
     9961 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    10021 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    10081 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    10141 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    10201 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    10261 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    10321 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    10381 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    10441 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    10501 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    10561 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    10621 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    10681 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    10741 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    10801 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    10861 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    10921 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    10981 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    11041 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    11101 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    11161 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    11221 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    11281 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    11341 aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca
    11401 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    11461 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    11521 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    11581 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    11641 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    11701 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    11761 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    11821 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    11881 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    11941 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    12001 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    12061 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    12121 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    12181 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    12241 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    12301 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    12361 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    12421 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    12481 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    12541 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    12601 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    12661 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    12721 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    12781 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    12841 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    12901 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    12961 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    13021 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    13081 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    13141 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    13201 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    13261 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    13321 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    13381 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    13441 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    13501 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    13561 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    13621 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    13681 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    13741 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    13801 gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc
    13861 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    13921 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    13981 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    14041 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    14101 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    14161 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    14221 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    14281 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    14341 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    14401 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    14461 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    14521 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    14581 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    14641 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    14701 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    14761 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    14821 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    14881 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    14941 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    15001 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    15061 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    15121 attattacaa tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt
    15181 aatcaaatag tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt
    15241 tttgcttata aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag
    15301 aaa
//
LOCUS       DACECZ010000062        14803 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.062, whole genome shotgun sequence.
ACCESSION   DACECZ010000062 DACECZ010000000
VERSION     DACECZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.062"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            137..1876
                     /gene="yfcC"
                     /locus_tag="IWO35_001722"
     CDS             137..1876
                     /gene="yfcC"
                     /locus_tag="IWO35_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ0316781.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2033..3217
                     /gene="iadA"
                     /locus_tag="IWO35_001723"
     CDS             2033..3217
                     /gene="iadA"
                     /locus_tag="IWO35_001723"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ0316782.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3328..3531
                     /locus_tag="IWO35_001724"
     CDS             3328..3531
                     /locus_tag="IWO35_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0316783.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3632..4294)
                     /locus_tag="IWO35_001725"
     CDS             complement(3632..4294)
                     /locus_tag="IWO35_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0316784.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4307..5809)
                     /locus_tag="IWO35_001726"
     CDS             complement(4307..5809)
                     /locus_tag="IWO35_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0316785.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6357..8036
                     /locus_tag="IWO35_001727"
     CDS             6357..8036
                     /locus_tag="IWO35_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0316786.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8163..8642
                     /locus_tag="IWO35_001728"
     CDS             8163..8642
                     /locus_tag="IWO35_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ0316787.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8861..9541
                     /locus_tag="IWO35_001729"
     CDS             8861..9541
                     /locus_tag="IWO35_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ0316788.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLIDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9549..10880
                     /locus_tag="IWO35_001730"
     CDS             9549..10880
                     /locus_tag="IWO35_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0316789.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10895..11857
                     /locus_tag="IWO35_001731"
     CDS             10895..11857
                     /locus_tag="IWO35_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ0316790.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11968..12306
                     /locus_tag="IWO35_001732"
     CDS             11968..12306
                     /locus_tag="IWO35_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ0316791.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12385..13092
                     /locus_tag="IWO35_001733"
     CDS             12385..13092
                     /locus_tag="IWO35_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ0316792.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13195..14676)
                     /locus_tag="IWO35_001734"
     CDS             complement(13195..14676)
                     /locus_tag="IWO35_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0316793.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4704 a         2545 c         3170 g         4384 t
ORIGIN      
        1 agaaaaaaaa caaaatagaa aaaagtgatt ttctaataaa ttattcatgt ggaaatgtca
       61 tttttactgt ataattcatt tgattatcca ttttttattc atcaaaatta ctgaaaaaag
      121 aaaggaagaa ataaatttga agaaaacaaa ttcttcagga aaaccgaaac gcgacttcac
      181 taaattatcg acaccccata cgtatgtcat tatttttggt gtagtcatct ttgcatggat
      241 actgactttt gtcgttccag caggaaagtt cagcacgcaa gatattgaat acaaagatgc
      301 aaatggcgaa acaagtactc gtactgtttt acgtcaagat tccttccgtt atgcttacga
      361 gctggataag tcgtatgtgt ttgatcaatt agaagaactg caagatcatc cagcagaacg
      421 tgaaaaactg gatgttccgg aaaaaggatt ggaaaaagtc attgctgacg gagaaaaaaa
      481 tcttacgcag gaaaaactag atgaaatctc cttgacagat gatgtgcttt acgatcagta
      541 cggcgaaaat atctatgata cttctaaaaa attgcataaa acagccaaaa tatggggaac
      601 agatgatttt ggcggctttg gtttcttaaa ttttgttttt gaaggactag tatctggaga
      661 taagtatgga tctgctgtag ggatcgctgc attgatcctt gttgttggag gtgcttttgg
      721 tattattatg cgtaccggag cgatcgacgc aggtatctat gcgtttatca gtaaaactaa
      781 gggactggag cgtttagctt taccgctatt attctttgct ttttcattcg gtggcgctac
      841 attcgggatg gcagaagagg taattccatt ctcaatggtg atggttccat ttgtcattgc
      901 gttaggatat gactcgatcg tagcagtaac cgtgacttat gtcgcatctc aagtcggaaa
      961 tgcgacctct tggatgagcc cgtttagcgt agcggtggct caagggattg ctggaatccc
     1021 cgtgttgtct ggtgcgacat tccgattgat tatgtgggta gttgttactg cattagccgc
     1081 tggttatatg atgatatatg cagaaaaaat ccgtaaaaaa ccagaaagtt cgttgactta
     1141 caaatcagat gattatttcc gttctcatat caaaaagaca tctgatgaaa ataaaaaatt
     1201 catgcttggg cataaattga tcttattaga gatgttagtt gtccttgtct ggatcgtttg
     1261 gggcgtgact caaaaaggct attacattcc agaaatcgct tctcaattct ttgtgatggg
     1321 attagttgct ggaatcattg ctgttctttt caaattagat ggaatgaaag tcaatgacat
     1381 cgcttcttct ttccaatctg gtgcagctga tttagctggt acagcaatcg tagtaggtat
     1441 ggccaaaggg attcttcttg tcttgggagg atctgatgca tctgttcctt cagctttgaa
     1501 tacgattttg catgggatcg ggacggcgtt gaccggtgtt ccagctgtca ttggtgcttg
     1561 ggcgatgtac atcttccaaa gtttgttcaa cttggttgtt acatcaaatt caggacaagc
     1621 agctttgact atgccaatca tggctccact ggcagattta gtaggtgtat ccagacaagt
     1681 agcagttctt gcttatcagc ttggttcagg ttttatggac gcttttacac ccgtatcagc
     1741 cagcttgatt ggtgtgctag gtgttgcacg gatcgaatgg gcaaaatggg caagattcca
     1801 aatcaagatg caaggattcc tgtttgtttt aggaactatt ttcatcatga ttgccattgc
     1861 aataggtctg cagtagtact ggagacctgt aaataaaaaa gtaatccaaa cagtagataa
     1921 ggactccaag aaccgtcatt ttacggtaaa atcaaaggag taaagatcct atcgtttgga
     1981 ttatttttta agatcctatc atcagttata cttaaagaaa gaggaaaaat gtatgaaaat
     2041 cattcgacaa attgaagtgt ttgcaccaga ttacttagga aaaatggatg tactgattgc
     2101 cggtgggaag attttggctg tagaagaaca gctagaaggc ggctacgaag gagtggaagt
     2161 agaagaactt tcaggtgagg gaaaagtact aaccccgggt ttcattgact gtcatttcca
     2221 tattttaggt ggaggaggag aaggcggtta tcaaaatcga acgccagaag tcacattgag
     2281 ccaattgact acagctggtg taacgacagt tgtcggttgt ttgggaacag acggagaagg
     2341 tagagatatg actgcgttga tcagcaaagc gaaaggtctg gaagcagaag gaatctccac
     2401 ttatgtatac gaaggttcgt accgattgcc ggtaaaaaca gtaacagatt ctatcatcaa
     2461 agactttttg acaattgata aaattatcgg aatcggagaa atcgcagtat ccgatcaccg
     2521 ttcttctcag ccaagttttg aagagttcgc tcgtgcagta gcagatgctc gtgtaggagg
     2581 catgttatcc ggaaaagctg ggatcatcaa tgtccactta ggcggaggaa aaagaaagct
     2641 agagctattg acacgaatcg tagaagaaac agaaattccg atcacacaat ttttgcctac
     2701 tcatgcaaat cgcacaccag aattatttga agcttgcgtg gcatttgcca aacgtggtgg
     2761 cacgattgac ttcactgcta gcgaagatcc tgatttttgg gagaagacag atggtgaagt
     2821 tcgcttcagc aaagcactga aacgattgat cgaggaagat gtttctttag ataatttcac
     2881 gatgacctca gatggacaag gaagcttgcc ttattttgat gaaaacaatc atttcctagg
     2941 gttaggagta ggaagtgcca aggcattgct agtagggatc aaagaagctg tgcaaaaaga
     3001 aagtatccct ttggaaatcg ccttacgagc tataaccagt aatccagcac gtatattgaa
     3061 attggataaa aaaggaaaaa tcgaaattgg tgctgatgca gatctgtgta tcttggataa
     3121 agaaacgctt gatattgata cagttatcgc taaaggagaa ataatggtcc aagaaaaaga
     3181 agtcaaagta tgggggactt tcgaaaaaag tttttaatca acagctctaa ctatcttttt
     3241 tagaaaacga gcgttctgtt tgtctgtatc tgttgttgat gttaccctga aggtagatca
     3301 ataaacaaac taagaggagg aagcacaatg gcagatttaa aaggacgttc taaagatatg
     3361 aaagataaag tagtcggtga aggaaaagaa gcatacggca aagcaacaga cgacaagagc
     3421 aaagaaacag aagggaaagc acaatcacta ggtgctgaca tcaaagaaaa agctcgaaat
     3481 ttaggcgacg aagtcaaaga caagttttct cgaaaagacg aaaaaaaata aaaaccactt
     3541 attactaatt gaatcaagca aataaaaaat ccgaaccata tctgcgagac agatatggtt
     3601 cggatttttt atttgatcat tatcctgttt tttaaacttc gattttttga aattttttat
     3661 tttttagttc aaagacttca tcacagcttt ttgctacttc aatctcatga gtcacgatga
     3721 taatacagcg attttgttca tgggcgattt cttggaaaag tttgacgata tcttgtgaag
     3781 ttgtttcatc tagatttcca gtaggctcat ccgctacgat tagttgatgt tcacagcaga
     3841 tagcacgaac gatagcgact cgctgctgct gacctcccga aagtttggtc acctgttttt
     3901 ttgctaatat ttcatcaatt cctacacgag ccagtcgatc taaagcaact tgtcgataat
     3961 tctcttgctt ggtcttagtg atttccattg ctgctatgac gttgtctaga gccgataaat
     4021 aaggaagcaa gttgtaagct tggaaaataa tcgagacatc atttttacga taacttctta
     4081 acccgatttt gttcagtgaa cgatcttgat agtatacctt tccttctttt ggtgcatcta
     4141 gtccagaaat aagcgaaaga aaagtcgttt ttccagaacc acttgctcct aaaatagcgt
     4201 acatttttcc agcttcgaaa ttcagttcta catctttata aagcgcatct tgcggatctt
     4261 gataccaaaa tcccaattgt tctgtccgta acatcaaatt cctcctttag tttaatagga
     4321 tttttcttgg gtttaatctt aatatcccta tagaagctaa aaagacagaa aacagactga
     4381 ttccaaatgc tactccggct aatagtccca attgtgcagg tttgacattg atttccaatt
     4441 cgctgacttg atcagaaaca gcaaatggat tattcgtggt tccttgttga ccatcgcgtt
     4501 gaccgccttg ttcttgtctg ttacctggca tttgtccacc gccaggagct tgctggtttt
     4561 gtgtcgtggt ttctgtctgt tggtcaagta gctgctgtcc cacggcattc gcgacgacgt
     4621 ttccgctaag cgaagcaact cctaatgcaa gaatcataca aatagccact tcggtaaaca
     4681 attggagaat gattttactt ctggattctc ctaaagatag caagacgcct aactcatgcc
     4741 gacgctcccg aatggtaatc ataatgatca gagtaagtat gataataccg gctacagcca
     4801 ctagtaaaac aacattttta gaaaaacttg ccacgttgtt taaaggttcc aacattgact
     4861 gatacatgga atcatttgat tgtaagctaa atgtatctgt gtcgatcagc ttctcagctt
     4921 ctttgagaaa ctcatccatt ttatctggat cagatagagt gtaaatagca gaatctatcg
     4981 tgttatcgtc agatgtccca tttagttcat ttgcaaaagt gtaagaagtg taaagtgtat
     5041 tcgacggatt catgaagttg aatttcatac ccatactgcc gcttgtttcg ctggattcat
     5101 aaattccttt gatcgtcatt tcataagtgg tatcttcatc ttcagtactc gtgattgtga
     5161 aagtatctcc gactgccagg tcatttgttt ctgctagagt agaatcaatg actacattgt
     5221 ttgtgccttt gtcgctgtca tctattcctt caccatccgt gatttttgct gttccatcag
     5281 aaaaattact attttgtgca gttgctgaca caccactgat ttgaaagtct gcttgagtca
     5341 tttgcggatt cccgcccatt cctccagcag gttcaccgcc tgcatcagta gtttctgaac
     5401 tgtcttcact tgaatcagaa ctagtgatgg cagttattcc atcttttgcc atagctgagg
     5461 cggaactttc aaaggaataa ctctttacat tatctaattc agctattttt tccgcgtccg
     5521 ttaaggaaac tggtgtaaga gaaaaactgc ctggatctgg acgttcttca gtggtagaac
     5581 cgcttgaaga agtttgttcc tgttttttaa atgaagcttc tcgattggca cttaaagtca
     5641 ctgttgctcc aacttctttt tgtgcttgat tcgctgcttc ttctgcagcg cttcgaatcg
     5701 tgaggccagc tagcacaaaa ataagaatgg ctgtgaacac ggcaaacaat aacaatgaac
     5761 gtccccattt tacttttgta cttttccatg cacgtttcat aaaattcata ttttgcatcc
     5821 ctcctagtag atgcatttga taatagacaa cctatcttaa agctagctta aatcttatgc
     5881 tgtttgcaca ccatcaacat gaggttctgt atatataaaa agtcactata gttattgcaa
     5941 aaaatgtggt aaaatgaaaa aagtatgttt caaaccgacg aagaagaaat gaaagaggaa
     6001 tagcaaaaga aatcctagat gctacagagg gatttttacg tgcatagttg ttctaacaat
     6061 tgctgatact agcgatttag tttatttgtt gtataataat ggggaacagc tgaatactta
     6121 tataagtatt ctggctatag cgtttcgcaa aagatccatt ttgttaatga agtatatcca
     6181 aaataaaatg cccatccaac tgtcagaacg cggatgtgct ggtaattttc ctgctgattc
     6241 tccataagtt gagaaatagt gattttgcat atgcttcatt ggaatggctc atatatttag
     6301 ataaagaaga aaatcgacat ctttaaactt ggatgtcata agaagaggtg aagtttgtga
     6361 gtaccattaa aattatccca ttaggcggcg ttcgtgaaaa tggaaaaaac atgtatgtcg
     6421 cagaagtggg cgaggatatt tttattttag attgtgggct aaaatatcct gaaaatgagt
     6481 tgttggggat cgacgtcgta atccctgatt ttacgtattt agaagaaaac attgatcgtg
     6541 tagctggtgt ctttttaaca catggacatg cagatgcgat cggggcactt ccttacttgt
     6601 tgtcaaaaat ttctgtacca gtattcggga cagagttgac gatcgagtta gcaaaattga
     6661 atgtcagtcg taatgaagca gccaaaggat ttaaggattt ccatgtggta gatgagcata
     6721 cagaaataga ctttggtgaa acagtagtga gctttttccg taccactcat acgataccag
     6781 attcagtggg gatcaatctg aaaacaaaag aaggtagtat cgtttatacg ggagatttta
     6841 aatttgacca aacggctatc ccaatgtatc aaacagatta tgcacgattg gcggaaatcg
     6901 gcaaagaagg agtcttagcc ttactaagtg aatcggcaaa tgcagaaaat cctcagcctg
     6961 tagcttctga agcccaaata gcagatgaag tatttgatac gatcaaatat tgggaagggc
     7021 gtatcatcgt cgcttgtgta gcaagtaatt tgcaacgagt gcaacaagta ttggatgcag
     7081 cagaaaaagc acaacgaaaa gttgtcttga ctggtcaaga tttcgaacga attattcgca
     7141 cagcgatgcg tttagaaaaa cttcaattac caagtgaaga tctgcttgtt acgttgaaag
     7201 acatgaaaaa ctatgatcca gaagaactga tcattttaga aactggccga atgggtgaac
     7261 ccatcaagtc tctgcaaaaa atggcaaacg gtacacaccg taatgtcaag attgaagaag
     7321 gagatttggt ttatatcact actacgccaa gcatcgcgat ggaaaccatc gtggcgaaaa
     7381 cggaagatat catttatcga gctggtggaa cagtgaagtt gatctctgag aatatgcgcg
     7441 tttctggcca tgccaatcct aatgacttgc aattaatgat caatttgatg aaaccaacat
     7501 attttgtgcc agttcaagga gagtatcgtg aattagctgc tcatgcagat ttggctcatg
     7561 cagtagggat gccatacaaa aatatctata ttactggacg cggagacgtg ctggaatata
     7621 agaacaagcg tatgagtgtt gcaggaacta caagtgcaga aaatgtaatg attgatggat
     7681 taggcatagg tgatatcgga aacattgttt tacgtgatcg aaaaatcttg tcagaagacg
     7741 ggattttcgt agcggttgtc actatcagtc ggcgagaaaa gaaaatcatt tcaaaacctc
     7801 aaattacttc acggggattc gtttacgtaa aagctagccg tgatctgatg aaagaaagca
     7861 gcaatatgat tgaaacaatc gttgagaaac atttagaaag caatgatttt gagtggagca
     7921 aattaaagca agacatccga gatcaattag gccgttactt atttgaacaa acaaaacgcc
     7981 gcccagtgat cttgccagtc attatggaag ctactcaacg tcgaaataaa aaataaacaa
     8041 aaagagtaag atgaacctct ctatagagtg ctcgtcttgc tctttttttg cgaaaaacaa
     8101 ataatttttg aatacagcat ccctcgcttt ataataaacg atagaataaa gggagtgaat
     8161 caatgatgag tcaaacaaaa gtcgaacgtt atttagaaga acaaaagatc agctatcaac
     8221 cacttgattt tagtcatgtg gtagaagaat ctttcacaga agaactacaa aaaagaggga
     8281 ttgatccagc tttgatatgt aaaactctcg tagtgaaagg aaacaaaact ggggtgatca
     8341 tcgcattgat tcctttgaac gatcacttag attacaaaaa aacgagacaa gtgacgaaag
     8401 atcgtaaagt tggttttcct ggaatggatt ttgtattaat gcatacagga tatccccacg
     8461 gagcaaacac ccctattgga ataaaactcg cacatcctga ttattgtttt ttagcagaca
     8521 gttctttaaa agaaaaagaa gaagtaattg tgtcaagcgg tgaaatcggg agaagccttc
     8581 ggataaaagt agccgattta gaaaagatcg tccatcctat ttacgctgat ttaattagtt
     8641 aagggacgag aagagaagct aagcagagca gagacctcaa acttgagatt atatctgctt
     8701 ggtacttggt gcatcctgta ttatctgtta caattgcttt gtacatactg tactatcaat
     8761 ccaatgaaaa tatatttagc agctatattt tttcattgat gtgcaagtga tgaaatcgtt
     8821 tttaataaaa aagatacaga gaaaacgcga ggtgtaaaat atgaaaatta cgtatcatgg
     8881 acattcctgt ttaacaattg aaatggaaaa tggccaacgc gtgatgattg acccgtttat
     8941 tacaggaaat ccattgatag acttaactgc agaagaagta aagacagatt ggttgctgat
     9001 tactcatggt catagtgacc acataggaga tatgattcct attgcaaaag aaaatcaagc
     9061 cacaattatc agtattgttg agattgctga ttatgcgaac agcagaggag tagattcatt
     9121 cggtatgaat atcggtggaa aacatgcgtt tccattcggc gtggtaaaat tcgtacatgc
     9181 acagcatagt tcaagctatg aagtagacgg tatcgttcag tatatgggcg agcctagcgg
     9241 aattattatt caagcagaag gaaaaacgat ttatcatgca ggggatactg cttatttcag
     9301 tgatctagga ttgcttgcag aagaattcga tattgatgtc gctttcttgc cgattggcga
     9361 taactatacg atggggccag aagatgcagc aaaggctgcg caaacaattc gtgccaagaa
     9421 agtagttcca atccattaca atactttccc tgtcattgaa caagatcccg aatcttttgt
     9481 agagttactg gaagatagtg aaggaaaaat aatgagcgtc tcggaaacgc tggaagtgta
     9541 ggcagaaaat ggcgacaaaa catgatttga ttttagaata tattgaaagt ttacctgttg
     9601 gcaatcgaat ttctgtaaga agcatcgcaa aagaattgaa tgtcagtgaa ggaacagcgt
     9661 accgtgcaat caaagatgca gaaaatgtcg gactagtttc aacaattcaa cgagtaggga
     9721 ccatccgtat cgaacgcaaa ttaaaaaaac atattgaacg tttgacattt ggcgaggtcg
     9781 tcaggatcat tgaaggggac gttttaggcg gggcggcagg attagacaag gtcttgaata
     9841 aattcgtcat cggcgcgatg actaaacatg ccatggaacg ctatattacg ccaggatcac
     9901 taatgatcgt cggcaaccgg gcggaagtcc aaaaattagc attggaagat ggcgccgcag
     9961 tattgatcac aggaggattc aatacgactg ctgagattgc aaagctagct gatgatcttg
    10021 aaatgccgat cttacgcacc tcttatgata catttacagt cgcaaccatg atcaaccgag
    10081 cgttaagcga ccaattgatc aaaaaagata tcatgcttgt cagtgatatc tatatgccag
    10141 ctgaaaagac tcattatttg cagcagacag atacgatttc tgattatcag cgtttgtcag
    10201 aagaaacgca gcattcgcgt tttccagtcg tcaaccgcca tcatcgtttg atggggattg
    10261 ttacggcaaa agatgtttta gggaaatcgc caaaccagat catcgatcgg gtgatgacga
    10321 aagaaccaat cagtgtaaag aaaacaatga gtattgcctc tgttagccat caaatgatct
    10381 gggatgggct agaagtcatg ccggtcgttt ccgatgactt gacattggaa ggcttgatta
    10441 cccggcaaga tgtaatgaaa gcgatgcagc ttgtccagcg acaaccgcag attgcagata
    10501 cgatttcgga tcagatatct ggaaatatcc atacgattga tactgaccgt gaaggaaatc
    10561 gtttggaaaa ccctaagttc aaattcgtag tagcaccaca aatggtcaat ggcgttggaa
    10621 ccatttcttt tggcgtactt agcgaaatca tttctgatgt cgcacaaaaa acaatggtga
    10681 tgaaccaaaa acgaaatatc ttgattgaac aagtgaacct acattatttg cggctgatcc
    10741 aattggaaag cgaattagac attcgtccac gtgtattgga aatcggcaga cgttccgcta
    10801 aactggatat cgaagtgttt attgaaaaca ccatcgtttc caaagcaatc gttgtttgtc
    10861 aagtaatgga acgttcttag aaagcaggag tgaaatgaca gtacagcaag aaatagtaga
    10921 tacaataaaa aaatacgata aaatcattat ccacaggcat ttgcgccctg atcctgatgc
    10981 attaggatca caagtaggat tagccgaact attacgggaa agtttcccag aaaaagaaat
    11041 acgtcaagta ggtgaaacga ttgaagggct tcgcttttta gcagaaatgc aagaagttga
    11101 agatgtcttc taccaaggag cgctagtcat cgtgactgat acagctaatt cacccagaat
    11161 cagtgatcaa agatatcagt tggcagaaaa actgatcaaa atcgaccatc atcccaatga
    11221 tgacccttat ggagatcttg tgtgggtaaa tacaaaagca agtagctgta gtgagatgat
    11281 tgcagaattt gctttgatgt ttcctgaatc tttaaagatg aacgttaagg ctgcacggtt
    11341 actttatgca ggaatcgtcg gagatacagg gcgctttttg tatccctcca cgacgtcccg
    11401 tacattagag attgcttcga tattgagaaa ttatccattt gatgcaagtg ctttgaacag
    11461 agagattgaa caaatgccga tgaaagtcgc taaactttcc ggttatcttt atcaaaatat
    11521 ccaggtggat gaaaatggtg caggaagaat aatccttccg caagaaattc ttgataaata
    11581 tggaatcgat gattcagaga cagcttcgat cgtttcactt cctggagtga ttgacgaagt
    11641 gctggcttgg gggatttttg ttcagcagcc ttcagggtat taccgcgtac gtttacgttc
    11701 taaggggccg atcatcaatg aattggctaa acgccatcat ggaggaggcc atccattagc
    11761 aagtggtgcg aatgcaaaag atttagcaga agtagaggaa atctataaag agatccaaga
    11821 tatatgccaa tcgtataaga gcagaaaaac agaatgatac ttcgttcgaa aacgtgcctt
    11881 ttgtagtaaa aatcacaaat gtatcttttc aagatcttct ttttccgtta caatagtgct
    11941 cactaaagat tttaaaggag aaagaagatg acattaccaa attgcccaga atgtggctca
    12001 gagtacacat atgaagatcg aggcttgttg atttgcccag aatgcgggaa cgaatggaac
    12061 aaagaagaac aagaaactgt catagaagac ggattagtgg tattagatgc aaatggaaac
    12121 cgtctgttag agggcgatag cgtgacagtg attaaagact tgaaagtaaa aggtgcgtca
    12181 ggtgccatta aacaaggaac aaaagtcaag aacatccgtt tagtagaagg cgatcataat
    12241 atcgattgca aggtagaagg atttggaccg atgaaactga aatcggaatt tgtgaaaaag
    12301 aattaaagaa tattcaaggt cttttcacat tcttttccta ctactaatga taaaataaag
    12361 taaacgaaaa ggagtgaaac gaaaatgtat ggttatggaa tgtatggttt tttagatccg
    12421 acatttcttt tggtcattct tggattagtt atttccggaa ttgcgtcagc gtacgtcaat
    12481 agtactttcc gaaaatacga tcaagtgaga agcaaaaaca atgtgactgg tacacaagca
    12541 gcacaatata tattacaaag tcaacagata aataatgtcg gtgttcaaca aattgccgga
    12601 gatctcacag ataactacaa ttcaggtaat aagatgttga gtttgtctca agcaactgca
    12661 cagtctactt cagttgctgc gataggtgta gcagctcatg aatgcggaca tgcggtacaa
    12721 gatgcttcgg gttatgtccc attgaagatt cgtgcagcac ttgttccagc agctaacttt
    12781 ggttcgatga tttcgttccc cttgattatg gtaggtgtat tgcttagctg gaactctacg
    12841 ttaatcaata tcgggatttt cgctttttct ttagcattac ttttccaatt ggtgacatta
    12901 ccagtcgaat ttaacgcgtc tcggcgagca atccaaattt tgagtgaagg cggactactg
    12961 acggaagaag aagtaccgat ggcaagacac gtattatttg cagcagcact gacttatgtt
    13021 gcagcagcgc tttcgacatt cctgcagctg ttgcgcttga tcttattgtt cggcggcaac
    13081 cgaagagatt aaaataaaga agttgtgaaa tcagcgcttt gatctgagct aataccgaga
    13141 gttagctgat tgaataccat ttatttggaa taagaggatg tgacaagaat tttgtcacat
    13201 cctctttgtt ttgttctcat aacctttttt catatacagt tcatgaaccg cttttattaa
    13261 cttgccaaat aacactgtat tgtgcgttgt atccagatag aaagtctccg tttcatctga
    13321 aattttctca tgctgatctg taatgatcaa atcagcatgt tctgaatatg cggtaatttt
    13381 aacagcatct ttgcgaaata actgctgcaa atggctttga agatgataat ttcctgtaaa
    13441 ctgtttggtt atctgcaaat ggatgagtaa ctgctctttc gtttctgttt gtagtaagtg
    13501 ttggatcaat tgactaaggt attgactgat ttggtttctt tccccatttt ttgaaaaaat
    13561 tgtatcgatt ttttctctta atttcttttc ctgtataaaa gaactagaat gattggacgt
    13621 ggcttctgga aagcaaagtg tagaaaaact actgctggat tcacccaaca gttcgaaaaa
    13681 aacaactgcc aacacaagaa aataaaaata aattggtatt ttattttcta aatggttgag
    13741 aggaaactgt tgagttaagt aagtcatcaa ttgaccgact tgtcgacaaa taaggttttt
    13801 gcttgtggaa aataaaaaac caatctcctg tttttgtcga aaagagattt gatttggaaa
    13861 aaaacaatag aaaaaataat taaagtaggc tctctctgtt tctgtctctt cacaagggcg
    13921 actcactgaa aaagaagtaa agtattctga ataatcatgt gtatgctgca ataagtccat
    13981 caattcagtg atttcagaag aagtttttgg aaaatatgcc tgttgtttgt ttcgtaatat
    14041 gaataaagtt aacagcaagt agatattttt ttcttgtttt gaaatttttt ctatttgctc
    14101 atctgtactg ttcattttta gtgcatttaa ttcaagtgag gaaaatggcc aatcgttacc
    14161 tccaaatgta tctgaaagaa aaaagtaaag atagagacgg atagataact catttccttc
    14221 aagagtacag caatttttta cagataggat ccgtaaatgg aattttttta aggaatgatt
    14281 gagctgtttt gtcagttttt cgagatagga gggcgatata taaagttgtt caagtaaata
    14341 tttttttggt acagcagttt gtggataatt gattagtaac atcaaatagt taaaacgatt
    14401 ggattctttt aggtagcata attttaactt agaaaaaaca gctaactttt tagtttgatc
    14461 ggtttctttg gtttggatct ctcctttatg attagaataa ataaattcat caatcaataa
    14521 aaattgcttg agtgatacat ttatttttcg gatcagttct ttgattgtcc gttttggaat
    14581 atgtaagtca ttggataatt cttgtaaaga acaattattt ttttgaatga ccatttgtag
    14641 aacaagtaac tctttttttc ttgcttcaat tagcataaat acctcctttt tattctgttt
    14701 ttgtatatag ttagacaaaa actgtgagtg gaaagtcatt tcagtctgtg ttattctcac
    14761 agagtgtcgc cttctttttt atataacgtt atttaatttt ttt
//
LOCUS       DACECZ010000063        14591 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.063, whole genome shotgun sequence.
ACCESSION   DACECZ010000063 DACECZ010000000
VERSION     DACECZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.063"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            12..785
                     /locus_tag="IWO35_001735"
     CDS             12..785
                     /locus_tag="IWO35_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316794.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(866..1435)
                     /gene="thiT"
                     /locus_tag="IWO35_001736"
     CDS             complement(866..1435)
                     /gene="thiT"
                     /locus_tag="IWO35_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ0316795.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(1501..1603)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1609..2580)
                     /locus_tag="IWO35_001737"
     CDS             complement(1609..2580)
                     /locus_tag="IWO35_001737"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0316796.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(2674..4047)
                     /gene="glmU"
                     /locus_tag="IWO35_001738"
     CDS             complement(2674..4047)
                     /gene="glmU"
                     /locus_tag="IWO35_001738"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ0316797.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(4550..5368)
                     /gene="purR"
                     /locus_tag="IWO35_001739"
     CDS             complement(4550..5368)
                     /gene="purR"
                     /locus_tag="IWO35_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ0316798.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(5535..6359)
                     /locus_tag="IWO35_001740"
     CDS             complement(5535..6359)
                     /locus_tag="IWO35_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0316799.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(6332..7012)
                     /locus_tag="IWO35_001741"
     CDS             complement(6332..7012)
                     /locus_tag="IWO35_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0316800.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(7035..7973)
                     /locus_tag="IWO35_001742"
     CDS             complement(7035..7973)
                     /locus_tag="IWO35_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ0316801.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(8218..9069)
                     /locus_tag="IWO35_001743"
     CDS             complement(8218..9069)
                     /locus_tag="IWO35_001743"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ0316802.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(9223..11796)
                     /gene="mprF"
                     /locus_tag="IWO35_001744"
     CDS             complement(9223..11796)
                     /gene="mprF"
                     /locus_tag="IWO35_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0316803.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            11937..12743
                     /gene="proC"
                     /locus_tag="IWO35_001745"
     CDS             11937..12743
                     /gene="proC"
                     /locus_tag="IWO35_001745"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ0316804.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(12822..14378)
                     /gene="rny"
                     /locus_tag="IWO35_001746"
     CDS             complement(12822..14378)
                     /gene="rny"
                     /locus_tag="IWO35_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ0316805.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT         4238 a         3176 c         2534 g         4643 t
ORIGIN      
        1 ggaggaacat gatgactgag tcagtttatg tacatattga taccacaagt aatgctgttt
       61 taacaaaagg gctgactgct actgactttg caaacagtat cgtgcatttt cctcaaaact
      121 tgctcttgct tgatccatct gcttctgctg gagaatatga atcccataca gggctgaaag
      181 taatccgtgg tacagaaaat attcagcgtt tcttttcctc ttctcgtaac aggcgtggat
      241 tcgatgaatt gaagtggatc gactttactg atttaacgat gctgaaagaa ttgacacctt
      301 tagaaatttc agaattatta tattttggtc atatgaaaac acatcttcat tcgccgtttt
      361 tttataaact acagaataat tttgtatact tcgatcttaa tgatcagctg aatcggattt
      421 attatcgcta tttagatgaa ttttatagga tttttgctag taaattaagt tcgatcttgt
      481 cagataagat caatgtaaaa aaatcttttt tcaaaaaaga aacacctgtt gaaaaaatca
      541 gtgtggaatt attgaaagaa atgcgtaatt tgatgcagga agggatcatt ttttcttttg
      601 atcaagttgg tttgatcgat ggtaaatatc gtatcccaat ctatgtgatt gaagattata
      661 tttggaaatt aaaaggcacc cggtatcgag aagaggaaat catcgctact ttaatctact
      721 ctgtcaccga tcgacactgg caattggtag aagatgccga tctttcttat ctgccattga
      781 agtagatcta tttcttatca aaaaataaaa aggaaacaag aaactaggac atgattctgt
      841 ctcaagtttc ttgcttcttt tttatttaca gctcctgtcg tttatctggc acaaaaatcg
      901 aaggagtcgt ttgttggatc cctaataaaa cgatgatcgt tacaatagct gtactcaacc
      961 cgctggcacc attcattacg aaagaaaaga gccaaggatt catcccccat agtgcatacg
     1021 ctccccagaa aaccactcct gcaataaaat gccaaaaata tctggaaagt gcgccaatca
     1081 atgcaccaaa cacgatgctt tttctaattt gtttcgtatt atcgtctaca tagccttttt
     1141 ggacatttga agccatgagc cctgctgcgc ctgcaaaagt ataggcaatc gggtactcaa
     1201 tcaacacttg aacgacactt aaataataaa cttgtcccaa cggaaaatgt aggatgcccc
     1261 aaatgaaacc tgccagcatc cctgcctttg tccctcttct taacgcaaaa agagtcaatg
     1321 gaatctgccc taatgaaata gaaaaactgc tgccgatctg gatcggaatc aacgacaaca
     1381 ccatcgctaa cgctgcaaca atcgttcctt ctacccaaac tctatttttt cccataaaaa
     1441 aacttcctcc tttgaataaa aaagtcacaa aggaggaagc agctttcgaa aatagtcaaa
     1501 cagctgttta ttcacaattc ctacgctcgc ataaccgata caggtacgaa gggtttagaa
     1561 atagattcaa tctcagccca attggactcc cctttgtgag tttatttatt atttattttt
     1621 tgtttcaaac aatggactaa ctggtttgtt ttcatggata cgtttgattg catctcccat
     1681 tagtccgcca acacttactt catcgatttt gtcgatttta cgatcttctg gaagatagat
     1741 cgagtctgta acaactagac gctcgatagc tgaatcttct attctttgta aagcagggcc
     1801 ggataatact ggatgtgtac atgaagcata cacactagtt gcaccagctt ctttcaaagc
     1861 attggcagcc aatgtgatcg tacctgctgt atcgatcata tcatcgatca gtacacaagt
     1921 tttgcctttg acttgtccaa tgatattcat tacttctgca acatttgctt ttggacggcg
     1981 tttgtctatg atcgcaatcg gagatttcaa aaattccgct aatttacgtg cccgtgtgac
     2041 accaccatgg tctggtgaaa caacgacaac atcatcgcct ttgattcctt tttctaagaa
     2101 ataattggca ataagtggcg cacccatcaa atgatcgact ggaatatcaa agaaaccttg
     2161 gatttggact gcatgaaggt ccaaagtcag cattcttgtt gcaccagctt tttgaatcat
     2221 gtttgctact aatttcgctg tgattggttc ccgtgcacgt gctttacggt cttgacgcgc
     2281 atagccataa taaggcatga ctacgttgat tgtttttgca cttgctcgtt tcagtgcgtc
     2341 aatcatgatc agcaactcca tcaagttatc attgactggg ctgctcgttg attgaataac
     2401 atagacatgc gatccacgaa tactttcttc aatgtttact tgaatttctc catcactaaa
     2461 ttgagtaaca gatgattttc ctaactcgac gccgactgct gctgcaattt tctctgctaa
     2521 tgggcgattg gaatttaacg caaaaatttt taatcttggg tcaaagtaat gttttgacat
     2581 ggggacctcc actttctttt tcacaactct agaattctac ctaataaata gtagtcatat
     2641 tttttaaata aatcaagtga ttttgcaaaa gtcttagttt agataaggga gtttttttgc
     2701 atatccttct ttattgactt gtcttgctct cgcaattgcc agcgcatatt ctggaatatt
     2761 gtctgtaatt gttgacccgg cagcaatcga actgttttta gctacctcga caggtccgat
     2821 gatattcgtg ctagaaccaa taaagctgtg gtcgccgaca gtcgtatgat gtttgttctt
     2881 tccatcatag ttgacgaaga caacaccaca tccgacattg atttcttctc ccagcgtagc
     2941 atcaccaaca taagtcaagt gaccgacttt agttcctttg ccgatttgag catttttcac
     3001 ttccacaaag tttccgatat ggacgttttc gccaatttca gcttttggtc gtaaatgtgc
     3061 ataaggtccc acatctgcat gtttcttgac atggctgctt tcaatgactg agttttcaat
     3121 gacgacgtga tcctcaatcc tactgtctac gatttttgaa tgtgacccga tgacacaatc
     3181 agaaccaatg actgtatttc cttggatttg tacacctgct tcgatcaatg tgtctggacc
     3241 gatttctacg cctgcatcga tgtatgtcgt tgctgaatcg acaaagctta cgccattgac
     3301 catatgcatc tggttaattc gctttctcat gatctcattt gctttagcta acgcgatccg
     3361 atcattgact cccattgatt cttcaaaatc atccgtttga taagcagcaa ctgtatggcc
     3421 ttcttctttt aagatttcga tgatatctgt tagatagtat tcaccttgag cattgtttgt
     3481 tccaactttg cttaacgcat cgaacaaagc ttcgttgtca aaacagtaag ttcctgtatt
     3541 gatttcttgt acaagagctt cttctggtgt tgcatctttt tgttccacga ttttttctac
     3601 gatcccaata tggtcacgga tgattcgtcc atatcctgtt gggttttctg cttgtgccgt
     3661 caagatcgtt gcacttgcat tttttccttg gtgatattca aaaagattgt tcagcgtttc
     3721 agtagtcaat aatggtgtat cgccactgat aactaacgtt gttccttttt ttccttccaa
     3781 gaaagaagca gcttgcaaaa cagcatgtcc tgttcccaat tgttctgctt gtaacgcata
     3841 tttactgcgt tctcctagtt gtgctttaac ttgttctgct ccgtgtccaa cgatcgtaat
     3901 cacttcatct ggtttcgttt cactgacccg attaatgata tgttctacca taggtttacc
     3961 agaaacggga tgtaaaactt tatataattt tgacttcatg cgggtacctt tccctgccgc
     4021 caaaataatt gcataacgtg cgtccaactg cgtcactcct aaatatagtt tgctgatttc
     4081 agtttatccc agtataccca ttttttcaac aaaacaagtg attttctttt atcccagcgt
     4141 tttctggata aaaaaataga tagaccataa cgggcctatc tatttggtaa ccggtgctct
     4201 cctgccaact cctcggtcac aagtcacact actcataaaa catgaaaagc tgttttttga
     4261 aatcgtttct tgtgacttag agttaaacgg taggcttaca acctcttcat taaacgtttt
     4321 tcaatcagct gaccttcggt attagctcaa taaacggcaa aatatgagga gcaatttttc
     4381 cgtttattga gctaatactc aggacaggac gctgatttca caacctctag ataaacggtg
     4441 ttcaaaagcc agtttctcgg tattaagcca aatttatgaa aaaatgtgga aagccatttt
     4501 cacaaaattt ttcttactac tcgaaactaa gcggcttttt cacaacctct tattctgaaa
     4561 aataatttcc tggcacaacc gtaatatttt tcgtttgagt atctacatct ttgacataaa
     4621 gcagtgacgt ataatcatcg atggcgcgat gaccgttgaa ttttgattcg gcaaatacag
     4681 tgatgccaac aagttctgat tcgaattctt caatcaagct cttcattccg ttgactgttc
     4741 cgccgccttt catgaaatca tccactacaa ggacacgtga accacgtttc aaacttctct
     4801 tagacagttc catcttttca attctttcag aagatccaga cacatagttg acacttactg
     4861 ttgatccttc tgttattttt gagtcccggc gtacaataac gaatggcgca ttcagataat
     4921 aggacacagc ttgggcaata ggtacgcctt ttgtggctac agtcattaca gcatcgattt
     4981 tttcaccaag gtatttagaa gcaatgattc ttccaacttg acgcagcagt tcaggttctc
     5041 ctaacaaatc agagagataa acatatcctc ctggaagcag acggtcttgt tctgatagac
     5101 gttctgctag agattcaata tattttttcg cctcttcata tgaaatttca gggatgaaca
     5161 atacaccacc tgcagcaccc gggattgttt ccaaggttcc ataccctcgt tctttaaatg
     5221 ttttcttgat gattcccaaa tcttcactga tagaagattt agcagattca taacgttcgg
     5281 caaagcttgt taaggaaatc aattcatgcg ggtgattcaa taaatactgt gtcatgtcaa
     5341 ctagacgttc actgcgacgt actttcacct attccaactc cttttaagaa attttatcgt
     5401 tttacattat ataagttttt cttcaaaaat tcgagcattt tatgaaaaaa taagaaaata
     5461 gttcggtatt tattaaaata aaaagaagcc tataaaaaat ttttcgtttt tataaggctt
     5521 cttttcttta taatttagtt attttttctt ctgaaggcaa ccaatattcg tttgatgata
     5581 tttacaatca ggaacaaagc aataaagact aatgtgattg ttgctcctgg aggtgtatct
     5641 agataaaacg agctagttag tcctgacagc atccctagaa atcctacaca aacactgata
     5701 gcaatcacga cattgaagct tttgcctaat cgcataccga ttgctgcggg aagaaccatg
     5761 attgcggata tcaaaagtgc accggcaatc gggatcatca cagcaatagc cacaccagta
     5821 atcacattga acatcatcga catcaaatga acgggtagcc catccacgtg ggcggtatct
     5881 tcatcaaaag tcagaacata catcggccgt ttgaataaaa agaacaatat accgatggca
     5941 acgaataaga ttcctaaaaa gatgacttgc tcccatgtaa tggtcacgat cgaaccaaac
     6001 agataggatt ggatacttgt tgctgaattc cctccgctca aattcatcaa tactaacgct
     6061 agcgccaaac caccagccat taaaatggca atggagattt ctgaataggt tcggtagatc
     6121 gttcgcagat actccaatat cacagctgct aatacaacga ctagcatggt cgtaagtgta
     6181 ggattaaggt tcaaaaagaa tcctaacgct acccctgcta aagagacatg agaaagcgta
     6241 tcagccatca gcgattgtct tctgatcact aaaaacacac ctagcatcgg ggcgattccg
     6301 gcaatgaaga tagcggcaag aaacgctctt ctcatgaatt catatgaaag caacgccatt
     6361 gagaatcctc ctttctcacc agtcggatct gtctgtccgc atactgtttg atatcttcat
     6421 gatcatgggt gatcattaga actgatttcc catggacatg cgcactatgt cggagaagac
     6481 gataaaattc atttctggat tcttcatcca tacctgttgt tggttcatca agaacaaata
     6541 agtcagggtc agtagcaaaa atacgcgcta aactgattcg ctgcttttgt ccgccagaca
     6601 attcacctat tcgccgattt cgcatatccc acatccctac agcttctaaa gcacgctgta
     6661 catgttgatg atctttttca gagagacgct tgaaccagcg gcctcttgga tatcttcctg
     6721 aacggactaa ttcgatcacc gtactaggaa aacctgcatt gaaagatgca acttgttgag
     6781 gaatataacc gatacttaat ttttttcctt cttgattttt ctcagcaatc tttatcattc
     6841 cttttgaagg tttcagtaat cctaacgatg ctttcaccaa ggttgacttg gctgctccat
     6901 tttctccagt taaaatgaca aattcacccg catctacatg ataagagaca tcttctagta
     6961 caggttcatc atcatagtaa aaggtcaaat cagatatttc gatataacgc accttacaac
     7021 ctccattcta aaacttaatt gatactttct tttaaagctt tcagattttt ttccatgaca
     7081 gaaatataat tttcgcctgc tttgatttgt tcattagtca agctttctaa aggattcaac
     7141 acttctagct tcactcctgt ttcattcgac aatgtctcag ctaccttcga agaggcattt
     7201 tcttcaaaat aaatcacttt gatgttgttt ttcttcacaa attcttttag ctcagctaag
     7261 cgacttggtg tgggttcctg atctggtgaa acgcccgaaa tcgctacttg ttttaagtcg
     7321 tattgattcg ctaaataagc aaaagcagca tgctgagtga caaatgtgcg gtttgttgca
     7381 tcttttagct catcggagta tttttgatcc agtgcttcaa gctttttgat ataagcatcg
     7441 gcattcttcg taaaagtttc ctttttatct ggatattttt cgcctaattc ttcagcaatc
     7501 gttctgactt cttttatagc taataccgga tcgagccaaa catgtggatc gtattcatgt
     7561 gaatgaccat gcccttcttc tgattcttca tgttcgtctg cttcttgcga ttctaaacgg
     7621 tcgattcctt tagctgcttc gatcatcaag gtctgtttgc tatccactga atcctttaac
     7681 gaatccacaa aaaattccat gtctgaacta ttatacacga aaacatctgc atcggaaatt
     7741 tctgctcttt cttttgcact tggttcgaaa tcatgcggct ccgttccagc agggatcaaa
     7801 agtttcacgt ttccttcatc ccctacgatc tcttttgtaa aatcatacat cggataaaaa
     7861 gtcgtaacga tcgtcaaatc ctcttttttg tctgcttcct tgttcgtatt tccacatgct
     7921 gcaaacagta aagcacctat cattaccgta gctgtcaaaa gatatttttt catttatatg
     7981 ctcctttcaa tgaacaaatc ggaattttac gatttattaa cgtgaactaa tgtatcaaaa
     8041 taaaaaaaca cagtcaatag aaatccatca atttctgatt taagcatgta tattcttgat
     8101 taaatcgtaa aatggatgag ttgctgctca tatctagcca tttcactaca ataaaaaact
     8161 gaagaatacc attttaggat tcctcagttt ttcatcacct attttacttc ttatatttta
     8221 ttgcagggtt cgtactaaat aaacttcctc acaaaaacct tttaacccat tatatactct
     8281 ctgcgctctt gaataatgtt gacacaaggc aaaaacggtt ggtccgcttc cactcatcaa
     8341 tgcagcatct gctccgtact tgatcatacg ctctttgatt tgatggatga ctggatgccg
     8401 ctttcccgtt atatcttcta aactgtttcc catttttttg atcatctgct gataatcttg
     8461 ctctcggatg gcttcagcaa gtccgtggat gtcttgatgt tggatggctt gcgtatctac
     8521 ttctctaaac actgtacgtg tagagacact cattcttggt ttgaccaaca caacccagca
     8581 ctgtggcata gaaggtaata cttggatttc ttcccctttg ccaccgacaa atgccgtact
     8641 tccgtagaca cagtaaggaa catctgtacc gacttccaaa ccgatttctg ccagttcttc
     8701 taacgaacag cccagattcc ataattggtt gatccctctt agagcagctg cacagtctgt
     8761 acttcctccg cctaatccag ctgctactgg aatattcttc ttaacaaaga tcttgacacc
     8821 ggttttgata ttgtatttat tttttagcaa taaagcagct tgataaacat tgttccgttc
     8881 atctaccggt aaaaatgctt tatttgtttc tacaatgatt ttgtcttctg ttatttcttc
     8941 aaaaaataga tgatctgcca agtctacgct cgacatcacc atttccaact catgataccc
     9001 atcttgccgt ctatataata catccagtcc taaatttatc ttggctggcg ctttttcaat
     9061 caattccatc ctctcaccca ttcgaaaagt ctaagtcgca agtattctat catagggaaa
     9121 aataaaagta tagcttatgt ctgcctagtg gttgattttg tcatcgtttc aacagctttc
     9181 acagaatttt ctcttcataa tccaaacaag ttctagtgat tcttataatt ctgtgagcac
     9241 ttcttctgcc aaactcggaa catttccttt ttcttttcct atcagactga gtagctgcat
     9301 ggtagcaaat ccgaaattac ttcctttcct ataagcaaaa taacgtcctt cccaatgatc
     9361 gacatatttt aatttctttt cataagcaga ctggaaggaa tagaaaaaat taccataatt
     9421 atatacgata tgcatgactt tttcttgaaa gaaagaaaaa tctgtttctc caacattagc
     9481 taatggagct aagcctagat tggcttccga gtagcccatt ttctcgtata caataaacag
     9541 attggctaac agatagtcag ctaattcctc agattcttta ggtaagaccc gcaacaaatc
     9601 atatcccgcc tgcttcttcg tcatcggctg agcggtgatg aagccaacga ctttttgatc
     9661 tttacggaca ataccgacac cgctagataa tacatatgat ttatcaaatc gtcccacaga
     9721 gaagtatttt tctcgtgcac cttgaagcca tttttgtgaa acttcattta cagcttgatg
     9781 taattcttcc ggcagttgat cgtaccaagt aaaatggtat ccttctttca tcaaacgttg
     9841 atattcaagt tgctgactca gccgagacaa gtcttctgtt gctagatcaa caatccctgc
     9901 ttcacctact ttcgtgaagt gaaaacccaa ttcatgaagc atcactacgt agctttctga
     9961 tatcttataa aacgcaagct gatagcctaa acaatctgct tgtttcataa actgattcgt
    10021 tgctgcccgc atcattttag gattgccaac tggatctcct aaaacaaagc atttgtttcc
    10081 tttgatctga taaccaaaaa cgacttgatc ttggtcgtct tcttggtaat aaaaataact
    10141 gtaacctgac aagcgaagct gatggcttgt agctgtgcct ccgtatgttt ccaataaacg
    10201 gtcaaaacgg tctttttcgt actcactgcc aagcttcgta gtatcttcag ctaaatattg
    10261 ataaagcgcc actaaagcta gcaatgagat gcccaaacca ataagccctg agaaccacac
    10321 atcatctgaa ggaaacaaaa taaaatgatt tcctgttatt cgaccgacta aaaagcgtcc
    10381 gctgtgatag cctgcaattg cgtaaacgat aaacaaaaag ccgaacaagc tagcatcaaa
    10441 gattatagct ccccaagaat aaacgaattt tgttcgataa aattcttttc tggataacca
    10501 cacagctaac agaatcaaga catataccaa tatcaactgc caagataacg tacgtgtgat
    10561 tgtatttaaa ataccaaaag atagtaaagc aatcgtaggc agaaatgcct ttttgacttt
    10621 tactgaaagt gctctagcaa ggccgagtaa taaaaagcca accaatagat tcactgtctg
    10681 atctaaaaaa ttgaatgaga acggcaataa tacttgaaat agccgactga ttgcagacag
    10741 attagtgatt gttgatagaa gtaccatcat gatccccgca aaatagacag cacttacgag
    10801 cacgatatgc gcaacctttt ggaaaaacag tcgcggaatg ttatctaaaa atcgattgat
    10861 tttgactcct gtatgtgtga tgaacaatag aagtcccgtc ataaaaggta gaacataata
    10921 aaacaaccga taatataata gccaaactac tgctacatct tgccgaacag acaattgact
    10981 cattcctaag atcatcaaaa cgtcaaaagt ccccattcca cctgggacca tcgtcagcat
    11041 tcctatcaat gtagcaatca tgaacatagg atagatcgaa agcatcgaaa tatctattct
    11101 catcaacttg ccgatgatca aaaataccgc aagggctccc gtccattgtc caaaggatgc
    11161 cagtagcaga ccaagcaagc cttttggata aaactcgaag aacaatgtct ttctctttag
    11221 ataagcaaaa aagaaaatga ctggtgctaa aaaactaccg ccgaaaagcc agatccaata
    11281 gcttcgaaaa atactttctg actgaataaa aaagacatca ataaatgtaa gaaacgacca
    11341 tacagacaaa ccagtcagca taaatagtgc gacttttgat acagtggcta aaaccatttt
    11401 tctatttgtc tgctttccat aaaaactagc ccgcagactt gctcctacca caccaccaaa
    11461 accggcgaga ttgttgatcg tgtttgttac ccacgcagca agaagaaact cttttctttc
    11521 catttttggt tttccttgtt tttctaagac tctgatcgtc acccaatcat acaacaacat
    11581 cggagtgaca cccagcagtc cagccagaat catgagcagg atcgtcatgc ggctttgttg
    11641 tcccatcctt tgccaaatat cttgccaact catgccatta gcaatatttg cgacttgatt
    11701 tgccacaaag actaacacgg agccgaaaaa aatcagtttt aaaatcagac tatgttcctt
    11761 tatccattgc agcatcaagt atcgtttatt tttcacattt tcccattcct aacttgtata
    11821 cttttgtgtc ataccccaat gttatcttat ttattttaat ttttccactt tagacctgtt
    11881 ctttgcttgc gatgatagaa aaagaaagat atcctaatgc taaaggagag gataacatga
    11941 aagtaggatt tatcggcaca gggaatatgg cacaagctat tattgatggt ttattaaaaa
    12001 aaggaacagt ctcgaaagaa gaggtgttcg tttccagtgg acattttgaa aatgcacaaa
    12061 aatttgctgc tgaaactggc gtccatgcat gcaaaagtaa tcaggaagtt gcatcacaat
    12121 cagatatcgt tcttttagcc gtgaaaccaa aaatcattga aaaagtattg gaagaactaa
    12181 gagaatatag ccagaaagtc ttattcattt ctattgcttc aggaaaatca ttaatagatt
    12241 tagaaacagc gcttgaagat cctcaggcct ccatcgtacg tgtgatgccg aatgtcaatg
    12301 tcagcgtagg agcaggcgtt tctgcgattt gcaaaaatga agctgccacg gaggaacagc
    12361 ttcagcaagc tacgatgatt tttgaagcaa tcggcagcgt ctatccttta gcagaatcag
    12421 atttcagtac attcattggc cttgctggca gctcacccgc attcgtttac atgctgatcg
    12481 atgcgatggg acggactggt gtactgaatg gtttgtccaa acagacagcc actgagattg
    12541 ctgcaaaagc aattatgggt tcttgtcaaa aattcctttc aagtgataaa aatccatggg
    12601 aacttgtaga ccaagtttct tctcctggcg gtacaactgt tgcaggtgta gttgccttag
    12661 aagaagcagg attcatccca gcaatcatca aaggaatcaa tacgacgatc gagaaagatc
    12721 ttgagatgtt gaataaaaaa taatgatagc aatgtttttt cgtgccttac aaaaaaagaa
    12781 gagctgtggc ttttgccgcg gctcttcttt tgtatttttt attatttagc ataatcagtt
    12841 gcacgagttt cccgaataac gattactttg atgtgtccag gataatccag ttcttcttcg
    12901 attcgtttac gaatatcacg tactaaacga acagcgtcta aatccgagat ttcttctggc
    12961 ttgaccatta cccgaacttc gcgtccagct tgaactgcgt aacttgattc tacaccggcg
    13021 aagctgttcg aaatattttc cagacttctt aaacgacgga tgtagttttc taatgattca
    13081 cttcgcgcac caggtcttgc tgctgagagc gcatctgctg cagctacaag gacagagata
    13141 acagaagtag cttccacatc tccatgatga gaagcgatcg cgttgattac tactgggttt
    13201 tctttgtact tggcagctaa ttccgcaccg atctcaacgt gagatccttc aatctcatgg
    13261 tcaagtgctt tcccaatatc atggagcaac ccggcacgtt ttgctaattg gatatcttcg
    13321 ccaagctctg ctgctaagat accagctaat tttgcaacct cgaccgaatg cttcaagacg
    13381 ttttgtccat aactagttct gaatcgcaga cgtccgatga tcttgattag atctggatga
    13441 agtgtatgag caccgacttc aaacgctgcc tcttccccgt attcgcgaat acgttcatcc
    13501 atttctttac gagatttctc gaccatttct tcgatacgcg ctggatggat tcgtccatct
    13561 tgtatcagtt tttccaaggt catccgtgcg atttctcttc ggattggatc gaatccggaa
    13621 agaacaactg cttccggtgt atcatcgatg attaaatcga taccagtcag cgtctcaagc
    13681 gttcgaatat tacgaccttc tcgtccaatg atacgacctt tcatttcatc attcggaagg
    13741 gtaacgactg aaacagtcgt ttctgaaact tgatctgctg cgcagcgttg aatcgctaat
    13801 gataataagt ttttcgcttt gcgatctgct tcttctttgg cacgctgttc agattctttt
    13861 accattagag ttaactcatg attcagctct tcttcagtcg atttcatgat gacatctcgt
    13921 gcgtcctctt ttgataaacc agcaattttt tctagttctt tttgttgatc ttcaatcatt
    13981 ttttcaacgt ctttttctcg ctcttcaatt aattgctgtt ttgaactaag tgcttcttct
    14041 ttactttcaa gtgagttctc gcgtttttct aacgagtcat ctttacgatt cagcgtttgt
    14101 tcgcgttgaa tcaaacgatt ctcttgagat tttaattcta acttactttc tctcagctca
    14161 ctttcaactt cagaacggta tttctgattt tcttccttag cttctaacaa tgcttctttt
    14221 tttagagttt ctgcttcttt ttttgcacta tcgatgattc cagcagctga tgattgtgca
    14281 ccagctattt ctttctcgtg acgtgacttt gcgaccataa accctaaacc aagaccgaca
    14341 attaaaccga tgatagcgag gagaatgttg aataccataa aatccacctc catactatct
    14401 ttttcatttt tatcatctat acagcttcta tgataaatta ttctaaactc gtcaatatgc
    14461 atcttcgcat atatgtgtac aaaaaacatg cacaacttta ttctaatgtt tgtaccccgt
    14521 agtgtcaact ttaaataaac aaagaaacgg ctttctactt attcattaaa cgatggtttt
    14581 taaaataaat g
//
LOCUS       DACECZ010000064        14559 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.064, whole genome shotgun sequence.
ACCESSION   DACECZ010000064 DACECZ010000000
VERSION     DACECZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.064"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            4..816
                     /locus_tag="IWO35_001747"
     CDS             4..816
                     /locus_tag="IWO35_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ0316806.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IWO35_001748"
     CDS             813..1613
                     /locus_tag="IWO35_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ0316807.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IWO35_001749"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IWO35_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ0316808.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IWO35_001750"
     CDS             3110..4819
                     /locus_tag="IWO35_001750"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ0316809.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="IWO35_001751"
     CDS             5031..9383
                     /locus_tag="IWO35_001751"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ0316810.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="IWO35_001752"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="IWO35_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ0316811.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="IWO35_001753"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="IWO35_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ0316812.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="IWO35_001754"
     CDS             11221..11514
                     /locus_tag="IWO35_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ0316813.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="IWO35_001755"
     CDS             11511..11822
                     /locus_tag="IWO35_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ0316814.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="IWO35_001756"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="IWO35_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ0316815.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="IWO35_001757"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="IWO35_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ0316816.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4963 a         2576 c         3247 g         3773 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccaaaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcaatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc tttagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaatccaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggatt
//
LOCUS       DACECZ010000065        14381 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.065, whole genome shotgun sequence.
ACCESSION   DACECZ010000065 DACECZ010000000
VERSION     DACECZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.065"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(27..923)
                     /locus_tag="IWO35_001758"
     CDS             complement(27..923)
                     /locus_tag="IWO35_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0316817.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(916..1596)
                     /locus_tag="IWO35_001759"
     CDS             complement(916..1596)
                     /locus_tag="IWO35_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0316818.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1597..2379)
                     /locus_tag="IWO35_001760"
     CDS             complement(1597..2379)
                     /locus_tag="IWO35_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0316819.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2372..3289)
                     /locus_tag="IWO35_001761"
     CDS             complement(2372..3289)
                     /locus_tag="IWO35_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0316820.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3453..3776)
                     /locus_tag="IWO35_001762"
     CDS             complement(3453..3776)
                     /locus_tag="IWO35_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0316821.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            4564..7791
                     /gene="fss3"
                     /locus_tag="IWO35_001763"
     CDS             4564..7791
                     /gene="fss3"
                     /locus_tag="IWO35_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ0316822.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            7904..8218
                     /locus_tag="IWO35_001764"
     CDS             7904..8218
                     /locus_tag="IWO35_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0316823.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            8231..8605
                     /locus_tag="IWO35_001765"
     CDS             8231..8605
                     /locus_tag="IWO35_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0316824.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            8615..8950
                     /locus_tag="IWO35_001766"
     CDS             8615..8950
                     /locus_tag="IWO35_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ0316825.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            9033..10373
                     /locus_tag="IWO35_001767"
     CDS             9033..10373
                     /locus_tag="IWO35_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0316826.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            10451..11125
                     /locus_tag="IWO35_001768"
     CDS             10451..11125
                     /locus_tag="IWO35_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0316827.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            11358..11792
                     /gene="dcm"
                     /locus_tag="IWO35_001769"
     CDS             11358..11792
                     /gene="dcm"
                     /locus_tag="IWO35_001769"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ0316828.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            11793..12500
                     /locus_tag="IWO35_001770"
     CDS             11793..12500
                     /locus_tag="IWO35_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ0316829.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            12490..12780
                     /locus_tag="IWO35_001771"
     CDS             12490..12780
                     /locus_tag="IWO35_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316830.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            13039..14223
                     /locus_tag="IWO35_001772"
     CDS             13039..14223
                     /locus_tag="IWO35_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0316831.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            14238..14357
                     /locus_tag="IWO35_001773"
     CDS             14238..14357
                     /locus_tag="IWO35_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ0316832.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         4597 a         2632 c         3018 g         4134 t
ORIGIN      
        1 tactagttct cactaatctt gtataattaa accctccgca acttgacgga gatggagaag
       61 atgcccccgt ctaatctagc ggtcatctct cctcccattc cttccaccaa tttttttgac
      121 aggtaaagcc cgattccgct agattccgta tttgtgcggc tggtattttc ggtataaaaa
      181 cgctggaaga ctttttcaac agaggtttgc gttcctggtt tgataggatt ggccactgtt
      241 aatcgaatga atggatcctc ttttgaaaat gtcacctttg cttgtccatc tgcataacgc
      301 aataaattct gtatgatatt ttgcagcact cgcagcaaca tgagcggatc tgctaataca
      361 gttgtattta tcgagtgttc agcgaacgct acctcgatat ttttctcaga aaattgttca
      421 taaaaagaga aaaatacttc ttccacctct ttagaaaaag aaagcgtatg gagatccatt
      481 tctttttgct tcgtctctat tcgagcaaga tcataaaact gatccgtcat actgagtaat
      541 cttgcaacag attgtttgat gatcgttaaa tacttctccc gtttctccgt atcctctacc
      601 tgttccagta attgtacata accgttaata gcagtcaaag gagttctgag atcatgagaa
      661 aggccgacga tcgatagtct gatgttttct tccatttctt gaaatgcttg tcctttttgt
      721 tcattttcat ccaaccatgt attcagtgca tctactaggt ccattagatt cttttctcga
      781 aattcgactg aaagacgacc accatattct tgtcgttcag aaagagtggc gattttctta
      841 gttaatctct tgatttccct ttgcaggaag aaaataaaac ttcccataaa gataacaacg
      901 agaagcaaca gccatttaat caaatctaaa ccccactccc cacaccgtat gaatatacac
      961 agcatgtgcc tttgctagct tccctcgaag attggaaata tgcatattca ctgttttctc
     1021 actatcaaaa tatggttctt gccaaactgt ttcataaaga tttgctcgcg aaaatacttt
     1081 tttcggatgt tctaaaaaaa gcttcagcag atcgaattct cttcctgtca aatggacagc
     1141 cacaccattc acatttacat ccctcgactc tttgtccagt aagatttcct tgtaatagat
     1201 gcgttgcgac agatctttta cttggacaga agtctgatga cgaagttgga tgccgattcg
     1261 taacaaaagt tcttcgatat caaatggttt cgtcacatag tcatccgctc ctgctgccaa
     1321 caaggaaact ttatctcttt gatcgctttt tgctgaaaga acgatgatcg gaacattcga
     1381 tgtctgtcgg atttctgcta acaatgcttc acctgaaagc cctggaagca tgagatctag
     1441 aaggatcaaa tcgacaggat atgtgcttaa caataatttt ccttccgtac ctgaataagc
     1501 atcaagaagt ttgtatctct gttcaagaac ctcctccagt aaggaatgaa cagcttcatc
     1561 gtcttcgata atcaaaatag tttccagcag gaccacctac ttaatgtcag atttttggaa
     1621 aataaacatt cctagagctg atgataccac gattgttact agactgacca gaatcatttt
     1681 cggtgcttgc gaataagttg cttgttgcgc aaagtccgta tacatgagcc acgtttccgt
     1741 aaaccactcc gttagaaatt ccaagccttt gatttttgcg aaaacaccgg gaatgaaggc
     1801 cgttcccatg atataaagaa aagaaagaag caaactgaca ctggtacttc tagtgatgaa
     1861 tgtcaaagcc actggaacag ataatgtccc taatgtcagc agaagcaata caggaaatac
     1921 ttttagaaca taatttccat agtttttgat tccctcaact tcaccaaatc cgaaagcaag
     1981 tgtgtaagca agcatggcta gtcccgtgat caaaaccacg ccaagtgttg tgatcaaaag
     2041 cgtaacgaga agtttcgaca cataataagt cattcgattc cgtcctaaag ataacgtatt
     2101 gcgaatcgta ccgttttgaa attcttcccc aatataaaag cacgcaaatg ctgcgaaagc
     2161 cagaagaatc catgcagcca ttttttctac cattaaaatc cctgttgcac cgttgatgac
     2221 tgtatccgtt gcttggttta attcattgcc gtattttgta tgcatatacc attcgttcca
     2281 agaataaaaa agcggaagta cgaccatcag tcctagtgag acccacattt tacggctctt
     2341 ccatactttt attttttcag cagttaatag attaaacatg tggattgcct ccagttaatt
     2401 gtaagaaata atcttccagt ttttgcccaa ctaggtgaat accatccaca ggaacttttg
     2461 cttctaccaa tgcttgattg atggctgcta cttggtcaat tccttcaaaa agctggataa
     2521 cacggctaga ttgtacaaaa taatccctat aaccaagaga gtcgagaacc gttactgctt
     2581 gttccgtcgc cgaagtgtca atttttataa attgacgaga ttcttgtgcc aattcttctt
     2641 tcgataattc tttgatcaag tgtccttcat gcaaaatacc atagtgagtg gcgatctgtg
     2701 acaattcatc tagcaagtgg ctagaaagca agacggtgat gccccgttct tttaccaaac
     2761 gttggatgat ttctcgcatt tctgccatcc ctgctggatc catcccattc gtcggttcat
     2821 ccaaaatcaa aaattctgga ttcgtgatca gtgcatttgc aatagctaaa cgctggcgca
     2881 tacctaaaga gaaattcttt gctttctttt tccctgtatt ttccaatcgc atcattttca
     2941 gcaggtcttc gatttctcta tccgacacac cgccgttcgc tgcttggata cgtaggttat
     3001 ctcttgcagt taattctgga taaagagcag gggtttcaat cgattgaccc atacgtcgtc
     3061 ttgctgcctg caaatccgtc gttccaaata attcgatcga accttcatca atcgagatca
     3121 aacccataat agttctcata aaggtagact tacccgctcc attttcccca atcaatccat
     3181 agatcattcc acgtttgatt tcgattgata ctttatctaa tgctttggca gcaccgtatt
     3241 ttttggttat accagttgct ttcaagaccg tttcatttcc aaaatccata cttatccttc
     3301 tttcttattg atgagtgaag tatagttgac gggtataaag aaagcagcca gaaatgtata
     3361 aagaaagtgt aaattttcca attgaatgtc aaaaaaaaca atcggacaat acgtgtcttt
     3421 cctattatca ggcagtttgt tatacgcttg tatcagtttc ctgattcttt cttttcgacc
     3481 aaagtcgttt ctctgtattc ctcttcactg aaaatttttg cttctttcag atcaagcttg
     3541 agtaatttta ccagaccggt tataaggata actatagcaa gactccctcc cagaaaaatt
     3601 agactgtctc gcagacgtgg ttgcgttgtc ttccaatcaa agagcgcacc aaacagtgct
     3661 aatccaagtg gactggccac catggtaatc gttgttatcg ttgacataag tcgtccttgt
     3721 atatgcgttg gaacttctcg ctgaagccag accatataaa aagcaaaaga aaacattaat
     3781 ccttaaaaac ggacttatga aaaataatac atgccgtttg ggatttacaa ttagctacca
     3841 aataagaggt tatgacaaaa gacttttcac aatcccttct tccgaataaa cggtgttctc
     3901 ctgccaactc ctcggtcaca agtcacaata tttatcaaac atgagaagca atttttgtaa
     3961 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcagaagc ggataaataa
     4021 tattgtttga tgatacttta tctttttccc gtttgtttat agatcgaatg atattataga
     4081 ccaagaatcc tacctattaa catagctaaa aacacaaaaa aggcactttc caaattgtgg
     4141 aaagtgccag aaaagttagg attaaggtaa ctgcgctcag cattgcttcg cccagtacga
     4201 ttctcgtaac tcgtgaactc gaacgataat cgcaattaac gttttgagaa ctgtgaagct
     4261 ttacgggctt tcttaagacc tggttttttc ttcgtatcat tgaataagtt tgaaaaacct
     4321 tgatttataa aggttttccc gtaaatatat tttattatat ttgattaaat ttgaaatgtt
     4381 tgtgttctaa aatgtgttct aatttaaagg cagtatattt cgtattttag attaaatttt
     4441 taggctcatt cattctcaaa tccattctat tcagtcaata aaaattttaa aaagacttta
     4501 taaacaaaga tgttcaagcg agcatcttat ttttttgtgc ttaatttgaa aggagaacat
     4561 ttcatgtata aaaaacaaaa aaagaaatta tgtgtattaa ctattttgag tttggctgtt
     4621 caagcaattg ttggagcaat tgctcctacg attacttttg ctgatgaaat tacccatcca
     4681 caaacggtaa ccgtagaact agatttagca catcaatatg ctgttgaagg aacttttagt
     4741 gatggtcgtc caatgtcaga agtcactgtt ccgcattatg cagtgtacaa cggctttaaa
     4801 caagatattt tctgtattga accaggagtc ccgatttaca atgagttcac tcctggctat
     4861 gagaaaaacc cgttaccaga tatgtccgat aaagccaaat tagtttccgt tttatggaaa
     4921 aaagtaggta cagatgcaga tactcatatt gtggcacaga agatgatctg gcaagaagtt
     4981 aatgggtata cccttcactc aataaaacga tcagatggta gtgccgtaaa tatcgcagcc
     5041 attgaggcaa aaataaatca agccattgac gattatcaga agaaaccaac cttccataat
     5101 agtacggcta aaacagtatt agggcaatcg actactgtga cggatacgaa taatctgaat
     5161 ttgtcagagt tcgatgaggt tgtggaaaat gccgcaaaca ttgattatcg tgtgaatggt
     5221 aatcagttga taatcacccc aaatgccaat tcaaaagaaa gtggtgtgct gacgcttaag
     5281 aaatctgctg gtaccggaac acctgtcgcc tataaaaagg ctggccaaca gactttaatg
     5341 gccggggcga ttgataaacc taatacctac acaatcaaaa ttgatgtgga aacggagggt
     5401 tctttaaaga tcaaaaaagt cgataaagaa tcgggtgatg ttgtaccagg aacggttttc
     5461 catttagact ttggaaaaac tttacctgca aaagacgtga ctactgacaa agaaggcatt
     5521 gctacattgg atgagattcc tcatggtaca aaagtaacca ttaccgaaaa atcagtaccg
     5581 gcgccttata cgattgatac cacccccatg actaccacca ttaaagcagg cgaaaccatt
     5641 tacgtgactt caaaaaacgc acgggaaaaa gggcaaatca ttctggataa aagcggagtt
     5701 gaaacaggga gtgatctttg gaatgacaat tattccttag ctggtaatac gtttgccatt
     5761 cgcaaagaca gtccaaccgg tgaaatcgtt caagaaatga ccacggatga aaacggtcat
     5821 gcggaaacac caaaagagat tgctaatgcg ttggaattgg gaacctatta tgtgaccgaa
     5881 actaaagcta gtcatgggtt cgtgaatacg ttcaaacctg taaaagtcga attgaagtat
     5941 gccaatcaaa ccgtagcccc tgttaccagt aacgtaaaag ggcaaaatca agaagtcact
     6001 ggcgaaacca ctttgacgaa agaagacaaa gatactgggg ataaagcaca aggtaaggca
     6061 gtctttgagg gtgctgaata caccttgttt actgctaagg atggaaaagc tgttaaatgg
     6121 agtgaggctt ttaaaccaga gttagcgaag ggaacgaaag gttctgatga aacggtcact
     6181 ttgactttag acgaaaagaa ccaagttgcc gttaaacatt tagctatcaa cgagtattat
     6241 tggcaagaaa caaaagcgcc cgaaggatat agccttgatg aaacaaagta tcctgtatcc
     6301 atcaaaaagg tggatgatag cgaaaaaaat gccgtgatta cccgaaatgt tacggcaaaa
     6361 gaacaagtga ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgcgggcact
     6421 accgaaactg gcttcaatga tctgaccttt aaagtgtcgc cattggaagg gaccaatgaa
     6481 atcacaggtg ccgaagatga agcgaccaca gcttataacg agcaattagg gtttgatggt
     6541 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaagcccca
     6601 gaaggttttc aaaagattac gccattagaa atccgttcta cttttaagga aaacaaagaa
     6661 gactttgtga agagtgagta tgtctttacc attaccgaac aagaccaaaa acaaccaatt
     6721 aaaacggtga ccgttcctta cgagaagctg acgaacaaag cattttctgt tagtttgaac
     6781 cgtttaatgc tctatgattt gcctgaggaa gaagatagtt taacttctct tgcgacttgg
     6841 aaagacggaa acaaagaatt aacttctctg gattctaccg agctagttga taaattgagt
     6901 tacaatctac atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggat
     6961 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtgat tgcagaaaca
     7021 agcgcaacgt tggcgaacaa agagaaaaca ggaacttgga aaattcagca taaattaacc
     7081 gccgaacaag ttttaaataa aactattgtc ttattcaact atgtgtatga aaataaggaa
     7141 gcttttgaag caggcgataa gccagtagcg aaggatgtta gcttgaataa ccaagcccaa
     7201 actgtcagtt gtacagtgga acaccatgtt tctatccaaa caaaggccca cctagaaaac
     7261 ggttctcaga cctttactca tggtgatgtg gtggatatgt ttgatgatgt gtcgattact
     7321 catgatgtac tggatggctc aaaagaagct ttcgaaacaa ttctgtatgc tttattaccg
     7381 gatggtacaa acaaagaaat ttggaaatct ggcaagattg attatgaagt aaatgataaa
     7441 gaattcacca aaactgtgct tgcgaaaaaa gtagataccg gaaaatatcc agaaggaacc
     7501 aagtttactt ttgcggaaat caattacgat aaagatggga atatcaatgg aaaacacaat
     7561 gaagatttga aagaaaaatc tcaaacctta acaccaaaag aagttccaac catactaagt
     7621 acgccgaaac aaccggaaac accaactgtt ccaagtggct ctcaagaatc tagtcccaca
     7681 gtgaagacct tccctcaaac cggggagaaa aattccaatg ttttactgtt cattggtttt
     7741 actttgattt ttgcgacggc gggctattat ttctggaatc gccggaacta aggtgatgag
     7801 atgatacaag taaatcctaa gaaccaacgt gcgcgaccgc ctcctaaaaa agtagtcgcg
     7861 ctatttttgt atccaaaact aacaatgaaa agaggaaaag aagatggaat tgaaatttgt
     7921 cgtgccagat atggctgaaa cctttggaaa gttaagttat gccggtgaag gagaaattat
     7981 aacagaagga tatggacgga atactacggt gattggtcgc agttatcatt tgtattccag
     8041 taagcaacgg gccgatgata ttgaggtggt agtagctgcg gaagccggtg aaaaggattt
     8101 tgatcaagac caaccgctaa aagccgtgaa tccccatttg gttgccaaag gctatgagat
     8161 tgagaatcgt gggtttactg attacgtgtt gtatgttgat gatttagtga aagcatagga
     8221 ggaaaagaag atgagattaa cagaaggtat tgtcgtagat tcaggtttaa catttgggaa
     8281 gttgcgtttt tccgcattac gccgagaagt acggaagcaa aatgaggatg gaacaattgg
     8341 taatgaggta aaagaacgaa cctataattt aaaatcctct gcacaagggc gaatgattca
     8401 agttagtatc ccagctaatg tacccttacg cgagtttgct tatgatgcag aggtagagtt
     8461 ggtcaatcca atcgtggata ccgttgccaa ttatgttttt cgagaaggaa ctaccgttaa
     8521 ttggttcatt aaggcggatg atttagtctt gaagcggcag ccgaatcaag gaaatcctac
     8581 gaaccaaaat gaaagaaaga aataggtgaa gattatggaa attaatgttg aacatatttt
     8641 agattgtctc gatcaatatg gaaaaggcga gctgactgag gaacaactcg ttaaagcatt
     8701 aacctctgat gaaaaaatgt ttctgattat acatcaaggt ttacttggag aaaatagcga
     8761 cagtgaagaa gactctgatg tactagactg gttaggagaa gaagagtctt tctttatagt
     8821 tgtcgaagta aatgaaaact tatgtcggca ggcagaatcc attttggaag aaattggtgt
     8881 ggagatgccg gatgctattg aaggcttttt aaatcaattg gttgaaacga aacaactccc
     8941 ctcctcgtag cagtaaatga ctagcataac tttttgaaat tgcctaatga aatcctattg
     9001 ggctttttcg attggaggtg gaaaccatca aaatgtacaa aggacatcgc atacgcgcag
     9061 gagatcagca cttggtttat cactttgttc ttggctggct actcgctctg ttcatcggtt
     9121 ggatgagcgt cttttatttt caagagttta gacaatttga tatttctaag ctatcacttt
     9181 ctactattga aatcgtttgg tctataaaag atttagtatg tctgcttgga agtctcgctt
     9241 tttcaggagc tatgatattg ctttatattc acttttttct tgatcattgg cgaagcttgt
     9301 ggcatcgaca aaagctagct cggatgattc tagagaacca ttggtatgaa gtgaaacaaa
     9361 ctcaaagtga aggctttttc aaagatttga atagtagccg aaccagagag actatcagtt
     9421 acttccccaa aatctattat cgaatgaaag atggattact gtctattcgc gttcaaattt
     9481 cacttgggaa ataccaagat cagcttttaa aattggaaaa gaagttagaa agcggactgt
     9541 attgtgagtt agtagaaaag gaactcaaag actcctatgt ggagtatact ttactgtatg
     9601 atatgatcgc caatcgaatt gggatagacg aagtagtggc agaaaatggg actctgcgat
     9661 tgatgaaaaa tcaagtatgg gcttatgatt ctttacccca tatgttaatc gctggtggca
     9721 cgggtggagg gaagacctat ttcctcctca ccattatcga agcactattg aagtcagatg
     9781 cagaattgtt tatcctcgat cccaaaaatg cagatttagc tgatttaggt acggtgatgc
     9841 ctcatgttta ttcacaaaag gaagaaattt ctgcttgtgt ggaagatttc tatgaacgta
     9901 tgatggctcg tagcaaggca atgaaagaaa tgtctaacta caaaacagga gagaattatg
     9961 cctatcttgg acttccaccc aactttttaa tctttgatga atacgtggct tatatggaga
    10021 tgttaacgac gaaagaaagt gcggtgattt tgaataagct caaacaaatc gtgatgttgg
    10081 gtcgtcaatc tggtttcttt ctgattttgg cttgccaaag gccggatgcg aaatatttag
    10141 gagacggaat ccgcgatcaa ttcaactttc gcgtggcttt gggtcgtatg agtgaactcg
    10201 gatattccat gatgtttggg gaggtcgaaa aaaacttctt tatgaagcac atcaaaggtc
    10261 gaggttatgt ggatacaggt ggcagtgtga ttagtgagtt ttatacgcca cttgtaccaa
    10321 aagggtatga tttcttagat tcaattagta aattagaaaa gatgaatgat tgagtgatct
    10381 tcaatactga ctttatactt caagtaacgt ataatacttt tatatagtag gaagaaaggg
    10441 gataattaat atggaaagta atgattctta tttagagtat gcaaaagcaa ataaggataa
    10501 gtttatttct tatattatca aagggaaatc ccccgaagtt ggacgagacg cagtttttat
    10561 ggcagggagt cctggggctg gaaaatcaga ggttgccatt ggattagctg agaattatga
    10621 taaccatgtg attattgatg cggattactt tcgcacccag tttcctgatt ataatggaaa
    10681 aaactctagt ctctttcaaa aagcatcttc ttggttggta gagcaggcat ttagatatgt
    10741 cttagatcat ggctattctt ttattctgga tgcaactttt gcaatattaa gtgctgaaaa
    10801 aaatatctcg cgggcgttga aaaaacattt tcgagtgacg attttctatg tttatcaaga
    10861 ccctaaaatc gcttggaaat ttacacaaga acgtgaaatt gcagagggac gtcatgtgcc
    10921 gaaaaaggca tttattaacg cttttttcaa atcaagagag aacattgcaa aagtaaagag
    10981 taaacatcca gatgtattgc ttcatatcat aataaaagat tatacgaata atatttccga
    11041 agttcattat gctacggata atctagaatt atttttacca ctagggtata gtaggaaaga
    11101 tttggaggtt caattacatg actgagaaag aacaagtaac gaaaattgta aagaaataca
    11161 ataagagcat tgcggattta tcggaaaatg ctactgcaaa agagttcaaa acggtgatta
    11221 aatatgtagc cgatcaggcc aatgaaaaac aacggaaatt agttggatta aataaaaaat
    11281 agtaggacaa attaataatg tatgagccaa gagcaaaatg ttcttggctt ttttctttgc
    11341 aatgaatgag gtaatgtatg agatttttag atttattcgc cggtattggt ggctttcgtt
    11401 taggtatgga acaggcgagc catcattgta tcggtttttg tgaaattgat aaatttgcta
    11461 gacgtagtta taaagcaatt catgatacaa gtaaggaggt ggaaatgcat gacatcacaa
    11521 gtgtatcaga tgagtttatt caatccctcg gaccagtgga catcctttgc ggcggatttc
    11581 cgtgccaagc tttttcaatt gcgggaaagc ggcaaagatt ttccgatact cgaggtactt
    11641 tattctttga aatcgctcgg ttcaccactc ttctccgacc taagctttta ttccttgaga
    11701 acgtccgagg attgctcaat cacgaaggag gggctacgtt cgagacgatc ctccgaacgc
    11761 tggatggatt ggggtatgac gtggagtggt aggtgcttaa ctcaaaggcc tacattcccc
    11821 aaagccggga gcggatattc cttatcggac attctcgaga cgcatgtacc gaacaagtat
    11881 ttcctatcat tggaccgtct tcaacatctg atcaaaacat cagaaatttg gtaaatatca
    11941 atccttccaa tcgaggaatg ggtggacaag tctatggttc agatggcgta gcgccaacct
    12001 taacaggtga tgaaggaatc aaaatcgcac tgccagcaag ggatggaatc tctgtagcag
    12061 gactattacc gggaaagttt gaacaaggta atcgagttta tgaagttact ggaaccgccc
    12121 ccaccttgtc aacgaaacaa ggtggtacaa aaatcatgat tcgaaaaaat gaaacagaat
    12181 atcaagaggt gaagccagaa gacagtgtga atctggcttt tcctaattcc acaagtcgcc
    12241 gaggtcgtct gggaaaacag agtgttcata ccttactaac aggagaccaa caagcgatcg
    12301 tcacggatca gtatcaaatt cggaagctaa cacctagaga gtgttggcga cttcagggct
    12361 ttcccgattg ggcttttgat cgagattctc aagtcaattc agatagtcag ttgtataagc
    12421 aagccggaaa ctctgtaaca gttccggtca tttttgatat tgccagaaga ttaaaagagt
    12481 tggatgtaca tggtttatga tgagttgatt ggagaaattt attgggtgat tggaaagatt
    12541 cagtcagacc cagaattaga agaacaattg cgacggttaa aatttgatat tcgcaagaat
    12601 ggtgtcaaag tcgctggcga tccatattta atgaatgagg agacggatgc tcgaattgaa
    12661 ataaatcaag taattgcaga atttgagcgg attgcggacc aagcaaaaga accagatatt
    12721 cgtcaatact tgttagaaat gaaagcagag ttggaagtaa atggtattac agatgagtaa
    12781 cctaaaccaa cacaactgac tgttaggcgc tgaccgcgga ggattcgcga agcagaaatc
    12841 ctccgtggtg ctgtgcctgt tcatggacgg cgttagccga ccatgatgtc tcacccccgt
    12901 atctaacagg ggggtacaaa aacacaaaaa ataagtaagc aaccattaag aatgcttgtt
    12961 gtatcaaaga tagtaagtaa tttgtatagg tgttaactca tttgaaaaag ttaacacctt
    13021 gaatttggag gtgagaactt ggcacaacgg aatttagatt atcggctatt aaaagaccga
    13081 cgcaacgaat acggcgtttc acaaaataaa ttggctacgg cttgtggact tagtcggccg
    13141 tacttaaatc agatagaaaa tggtggcgta accgcatcta taaaaaccat gaggaaaatt
    13201 tttgaccaac tggaaagctt taatcccgat ttgcctttga cgttactgtt tgactatgta
    13261 aggattcgct ttcccacaac ggatgcacga aaaattattc aagaaattct ccatttgaaa
    13321 tttgagtata tgctccatga agattatgcc ttttactcct atcaagaaca gtatgtcatg
    13381 ggtgatattg tggtgatgtt gtctcatgaa gaagataaag gtgtgctttt agaattaaag
    13441 gggcgaggtt gccgacaatt tgaaactttt ttactcgctc aaaagcgtag ctggtacgac
    13501 tttttcgagg attgtctaaa ggccggtggc gtgatgaaac gcttggactt ggcaatcaat
    13561 gatcgagtgg ggctattgga tatacctgag ctaacgaaaa aatgtcagaa ggaagaatgt
    13621 atctccttgt ttcgtacctt taaaagttat cgttccgggg aacttttaaa ggctgatgaa
    13681 aaggacggca tgggcaatac cttatatatc ggcagtctca agagtgaggt ttatttctgt
    13741 ttgtatgaaa aagactatga acaatatatc aagttaggga ttccattaga ccaaactgag
    13801 acgaaaaatc gttttgagat tcgactgaaa aatgaccgtg cttttcatgc gattcaagat
    13861 ttgttaaaag gtcgtagcat tgagagcacc acgttttcca ttatcaatcg ttacttgcga
    13921 tttgcggata aagtagaagg taaaaggcga accaactggc ccttgaatga acaatggggg
    13981 cgttttattg gacggaatcg gaaagagatt caattaactt ccgagccaaa accgtatacc
    14041 attgaacgaa ccttgaattg gctaggccga caagtcgccc ctacgtggaa aatggctaag
    14101 gagttggatc gattgaatcg gacaacctat atccaggata tggtacagaa tgcacgctta
    14161 tcagaccgac ataaaaagat tttggagcaa caaagtatgg caattgaaaa tttgattata
    14221 tgaaaagagg aatcgtcatg ggaattgtaa aagatatttt tttggtattt ggtggcagta
    14281 tgcttggtgt tactgtgatg tgcttgatga atgcaagtgc agcagcggat cgagcaatgg
    14341 aaaagaaagg aaagtgacaa gatgaatttc ggacaaaatt t
//
LOCUS       DACECZ010000066        14140 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.066, whole genome shotgun sequence.
ACCESSION   DACECZ010000066 DACECZ010000000
VERSION     DACECZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.066"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            123..1409
                     /gene="efmA"
                     /locus_tag="IWO35_001774"
     CDS             123..1409
                     /gene="efmA"
                     /locus_tag="IWO35_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ0316833.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(1547..2146)
                     /locus_tag="IWO35_001775"
     CDS             complement(1547..2146)
                     /locus_tag="IWO35_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ0316834.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            2550..3671
                     /gene="mnmA"
                     /locus_tag="IWO35_001776"
     CDS             2550..3671
                     /gene="mnmA"
                     /locus_tag="IWO35_001776"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ0316835.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            3917..5338
                     /gene="arcD"
                     /locus_tag="IWO35_001777"
     CDS             3917..5338
                     /gene="arcD"
                     /locus_tag="IWO35_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ0316836.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            5352..6470
                     /locus_tag="IWO35_001778"
     CDS             5352..6470
                     /locus_tag="IWO35_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ0316837.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(6530..7822)
                     /locus_tag="IWO35_001779"
     CDS             complement(6530..7822)
                     /locus_tag="IWO35_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0316838.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            7972..8532
                     /locus_tag="IWO35_001780"
     CDS             7972..8532
                     /locus_tag="IWO35_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0316839.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            8661..9038
                     /locus_tag="IWO35_001781"
     CDS             8661..9038
                     /locus_tag="IWO35_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ0316840.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            8971..11043
                     /locus_tag="IWO35_001782"
     CDS             8971..11043
                     /locus_tag="IWO35_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0316841.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(11254..12201)
                     /locus_tag="IWO35_001783"
     CDS             complement(11254..12201)
                     /locus_tag="IWO35_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0316842.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            12447..12911
                     /locus_tag="IWO35_001784"
     CDS             12447..12911
                     /locus_tag="IWO35_001784"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ0316843.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(12973..14016)
                     /locus_tag="IWO35_001785"
     CDS             complement(12973..14016)
                     /locus_tag="IWO35_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ0316844.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         4588 a         2588 c         2984 g         3980 t
ORIGIN      
        1 gtaaagaaat ttaaattgaa acgattgtta tttagggaaa aaggtatttt ttgaaaaaag
       61 aatcccgtat aatgattgtt gtgcaaacgc tatacaaaat gaaaaagaaa agagggatgt
      121 ttatggaaaa tgaacagtcg gtcgttttaa cgaattggaa gagaaattat ttgttctttt
      181 tatcgggaca gtttttgtca ggaatcacta gtatggtcgt ccaatatgcg atcatctggt
      241 atttgacaag agaaaccggt tcggcgacaa ttttgagttt tgctacttta ctaggaatga
      301 ttcccatggt tttactaagc ccatttgtag gaccgctagt ggatcgctgg gataaaaaag
      361 ctcttttgat tgtcacggat attattgtag cgattttcgc gcttatttta gcagtagtcg
      421 gaacgatttc agaatccttc ccgatttggc tagtttttgt gtctttgttt atgcgttcgg
      481 tcgcacaaac attccaaatg ccaacgatcc agtcgattat gccgacaatc gttccatctt
      541 ctcacataac aaggacgaat ggacagttag gaatggtcca atcagcgaat ttcatcattg
      601 cgccagctct aggtgcggct ttgttttcag tcgttcctgt caattacctt attttattag
      661 atgtattggg ggcagttttt ggtgtcggct tattgatttt tgtgaagatc ccaaaagttt
      721 ctcctgaaat attggaagtt ccgcttacta tttttaaaga tgcaaaattt gggctgcagc
      781 aattgatgga taataaaggg ttatggtaca taacgatcaa tggtgcattt gtgatgctgt
      841 tgttcatgcc agcaatcagt ctgtatccat tgatgacgct agattacttt ggtggttccg
      901 ttggacaggc gggagcagtg gaagtagtct acgcagtcgg aatgctctta ggcggtgcac
      961 tgatcagttt tataggaaca tggaaagacc gaatgaaacc tattattata gcctacatca
     1021 ttatgggcct gacgatcggt gcaagcggat tggttccaaa cgatagtcaa gggttcttgt
     1081 actttcttat cttgaatgca ggagcaggtt gtgcaacgcc atatttcaat acactgctga
     1141 tggcaatgat ccagcagagt tatgaatcga atgttttagg tcgtgtctta gggaacttca
     1201 actctttgat gaacctggca ggtccaattg gattattatt tgcaggacca ttagctgatc
     1261 gtttaggggt agaaaaaatg ttcttgtttt ccggaatcgg gattttatta tgcggaatcg
     1321 ttttattttt aacgtcagct gcccgaaatt atgataaaga attacagaaa aaactggtaa
     1381 aagaacacca tgagcaaaaa gacgaatagt atggaatgga gttgtgacag aagtcttgtt
     1441 acaactccat ttctgatcct aatgttcctg tggcaataag cctactcaaa tgaaaaatat
     1501 gagtaattat ctttatttgt ttaatacttg atatagggca gttttttcac gacctatcgt
     1561 acttcctctt tgcttcatag atttctttga tttgttgctc agataacgga gtgccaaatg
     1621 gtagtttttc ctttaagagt tctctttccg gtgcgtctac gcctacattg acatcgttct
     1681 cgataggtac gctgtaatga tggatatgtc catgaagatt gcggatagtg tcgttaggcc
     1741 cgagcaacat cggataatga gtcaagtaat attgttgatg gtcgaattta acgatggcac
     1801 ctacatcata aaaataaaat ttgggacttc cgtcagtaag cctaggatca tttttttcta
     1861 aataacggaa aaaagcgcga ctgtcatggt tccctttgat caagtggatt gttccttgca
     1921 gttgagtgac cagttccaaa atttctgcat ttgctttttc atattgcgga tgcatagcaa
     1981 ggtcgcctag atgataaaca tggtcggttt cttttattac tgcattccag ctagtgatca
     2041 actgttggtt catttcatcc acatttgcaa agagccgcgg agcaaagtcg ttttttccta
     2101 ataagcgttc atggaaaaag tgcatatcac tgataaagta attcatgtat cttttgcctc
     2161 ccttttttct tagagtaaac aaaatcactt atctgagcaa ctcataacat ctaaaaagaa
     2221 aaggttgtaa atgagacgtg cagctttaag cgataagaag caattttgaa aaatagtttt
     2281 tcacaattta taaaattatt ccttattgcc gaaaggctgg ccctttttat gagtatgcca
     2341 attttttata tcgaatgctc aaaagatgag tgattttttg gttgactatc atgtttgtgt
     2401 actgaatagg tccagtagaa cagtattttt acgacttgat aattaaggga tgaaattttc
     2461 agaaatatga cgtataatgt aaaatactga aaagttgcga ccttcaactc gtagatgttt
     2521 cagaatctaa ttgaaatgat ggtgatatca tgacagacaa cagcaaaaca cgcgttgtcg
     2581 tagggatgag cggcggtgtc gactcatctg taacagcact tttgttaaag gaacaaggat
     2641 acgacgtgat cggaatattc atgaaaaatt gggatgacac ggacgaaaac ggtgtatgca
     2701 cggccacgga agattataag gatgtagcga aagtagctgc tcaaatcggg attccttatt
     2761 actctgtgaa ttttgaaaaa gaatattggg atcgtgtatt tgaatatttc ctagccgaat
     2821 accgcgcagg acgtacacct aacccagacg tgatgtgcaa caaagaaatc aaattcaaag
     2881 cattcttgga ttatgcaatg gatcttggcg ccgattatgt agctactgga cattatgcgc
     2941 aagtcactcg cgatgaaaac ggcgtggtcc acatgcttcg tggagtagac aataataaag
     3001 atcaaactta cttccttagc caattatcac aagaacagct ttcaaaaacg atgttccctc
     3061 taggcggcat ggaaaaatca gaagtacggg caattgctga acgcgcaggc cttgcgactg
     3121 ctaagaaaaa agattcaacg ggaatctgct ttattggtga aaagaacttc aaacaatttt
     3181 tgagcaacta tctgccagca aaaaaaggaa atatggtcac tcttgatggt gaagtcaaag
     3241 gacaacatgc tggcttgatg tattatacga ttggtcaacg tcaaggacta gggatcggtg
     3301 gcggaggaga ttcgcaagaa ccctggtttg ttgtaggaaa agatctagcg acaaacacat
     3361 tatatgtggg gcaagggttc catcatcctg cattgtatgc cacaagcttg gatgcaagtg
     3421 aaatccactt tacgacgaat gaaccaatgc caaaagaatt caagtgtaca gcgaaattcc
     3481 gttatcgtca gcaagacgtt cctgtgacag ttcgcttatt ggatgataat cgtgcagaag
     3541 tcgtattcga cgaacctgta cgtgcaatca ctcctggaca agcagttgta ttttatgatg
     3601 gaatggaatg tctgggtggt ggtttgatcg accacgcata tcaagaaaca aaagttttac
     3661 aatacgttta agcgatatta aatgaatcaa aacagctatg gctaacaaaa gaaacacctt
     3721 ttcttttgga tagcatagct gtttttttgc aataaaatta atttttattc attttatact
     3781 tgaaatactt gtgtaaaaag ttcataatat ccaataatat gaataaagtt gctttttgtt
     3841 aagcaagagc agttgcaaaa tagaagggaa tcttttgcaa gtgatttgtt caattttata
     3901 agtgagggtg atggaaatgg aaaagaaatc gacgggtatt agcaaatggg gactggttgc
     3961 tctggttgtc agttcgtcaa tcggaagcgg tgtgtttggg atcacaagtg atctggctag
     4021 ttctgcagct ccagggccag cgatcctttc atggataatc gttgggattg gtattttagc
     4081 tttggtatta tcgctgaatc atttaggaga aaagcgtcca gatctagatg gtggtatttt
     4141 cggttacgcg gaagcttcat ttggtaaatt aggtggcttc atcagtggtt ggggttactg
     4201 gttgtctgcg tggctgggaa atgttgcttt tgcaacgatg ctgatgagcg ccatgggaga
     4261 atttttcccg atattcaaag gtggacaaaa tgttccttct attttatttg caagtatttt
     4321 tatttggggt ctgactttat tagtaaacaa tggagttgaa ggagcgagcg tcatcaatac
     4381 aatcgtgacg atctgtaaat tagtgccatt attcctattt ttaatatttg tatttatcgc
     4441 atttaaagtg aatatattca ctgctgattt ttggggtaac gtatctgata atctagtgaa
     4501 tggaacaggg cagcaaggaa cgatctggtt gcagattaaa ggttgtttga tggttatgat
     4561 gtgggtcttt gtagggatcg aaggcgcaag tgttttagcc aaccgcgcga agaaacgttc
     4621 agaagcccag caagcatcga tcattggtct gctttgttta ttgtttatct atatccttgc
     4681 ttcgatcttg ccatacggcg tcttatctca agaagagtta gcaactattt cacaacctgc
     4741 tatggcaaat attttaaaag gaatcgtggg aaattgggga gcagcgctga tcaatatcgg
     4801 cttgattatt tcgatcattg gttcttggtt gtcttggacg atgctcccag cagaaacaac
     4861 gatgttgatg gctcgagacg gtctgttgcc gaaaggttgg ggaaaaacga ataaaaaaaa
     4921 tgccccaact tattcgttag ttgttactgc aatcttgact aatctattct tattgacttt
     4981 cttagttact gattatgcct atcaatttgc ctattcttta tgtacagcgg caattttgat
     5041 ttgttatcta cttgtcggga tctatcaagt ccagttttct ttaaaagaag gaatcaaaaa
     5101 acaagtagtg atcggtggga ttgctgtagt ttttgaattg atgggcatca ttttagcagg
     5161 attttcttat gtccttttat gtagtattgc ctatcttcct ggtttttgct tctactggat
     5221 cgcttgtcgt gaaaccaaac atcaaattac gggaaaagaa aaagtaatga ttgggctgat
     5281 ttgtgtggcc gcactgcttt caatcgggtt gcttgctgta ggctggatca agatataagg
     5341 gggaaagaaa catgaaaatc gcaaatttcg gtgtagaaga atggctaaat aaatgggaaa
     5401 agaaagcggt atatgatatt gcacaaagct cgatcgaagc attgacatta gacgaactcg
     5461 ttggcttaga tgggactagc gtaagtaaat ttttcgaaaa acgcaaaaac gaaccattgg
     5521 actatggctg gatcgaagga tcagatgttt tcaaacaaga agtcgcttct ctttatcaaa
     5581 ccgtcgatcc ggaaaacatc ttacaaacga atggagcgac tggtgctaat cttttagctc
     5641 tatacgcgtt agtggaagca ggagaccatg ttgtttcgat gcttcctact tatcagcaat
     5701 tatatgacat ccccaaatct ttaggtgccg ctgttgactt tgttcatttg aaggaagaag
     5761 aagattggca gtttgattta gaacagctag aagccttagt caaacctgaa acaaaaatca
     5821 tctgtttgaa tagtgcaaac aatcctacag gcacattgct agatcggaaa actttagaaa
     5881 aaatcgcaga aattgctcgt acagtagatg catatgtatt gatagatgaa gtatatgcgc
     5941 ctttaacgga taaaggtgaa ttcttgtcga tcgtagatgt ctatgaaaaa ggtatagcaa
     6001 ccaattccct atccaaaacc tactccattc caggtattcg tattggctgg acagctactg
     6061 gacctgagtt ggcagaagtt tttcgaaagt atcgagacta cacgatgatt tgcggcggag
     6121 tcctgtcaga tgatcttgct gtacatgcat tgaagaataa agaaaaaata ttggcgcgaa
     6181 acaaaaagat cattacagag aaccttgtga ttttaaaaca atgggtagcc aatgaaccga
     6241 aagttgaact tgtcgctccc aattacgtct ctacttcttt cattaaatta gccatcgaag
     6301 aagatgacca gacattttgt atcaatttat tagaagaaac aggagtacta ttagtaccag
     6361 gaagtgcctt tgatttaccg aagcatgccc gactaggtta ctgttgtaaa aaagaaacct
     6421 tggaaaaggg attattcctc ttatctgagt tcttgaaaaa acaggcctaa acaaaaacac
     6481 tgcaaggaca gtctgtgaca actgttcaag cagtgttttt catttacgct tattgtctat
     6541 gagaaggaga gtgccctgaa gctatggaga ctcctgaagc tttttcttct gcagtttctt
     6601 gctgatggat ctcttgtgta ttgtgtcgta caagcaagaa gttgaccaaa acaagaagag
     6661 aagtcgaaag aaagacgcca cgataaccaa atgcactgga aacactggag ccgatcatag
     6721 gccctactac gttaccagtt gcttggaaag attggttata actgaagatc cgtccagcag
     6781 cttgatgagg cgaatatttt gtgatcaatg cttggacagc agggagcaga caagcatcag
     6841 agataccaat caaaaaacgt aaggcagcta attgccaaac attcgtgacg aatgccattg
     6901 ggatataaac cagaatcgca aaacccaaac caatcgcaag tattctttca ctcccaatct
     6961 tgtcacctaa gcgaccaaat cttggagcgg ctatcaaagt ggcaatccca ggtatcgaag
     7021 caatgattcc gctgatcaac gtaacattcc catgtccatg cagcaattgg cgaatgtaca
     7081 gactgatgat tggactaatg gaattgttgg atgcttggat gatcatcgtt gtgacgaaca
     7141 taccgatgac gacgtgtgga tatttcaagt ctttgaaaat ctgtttggca gaaaccatgt
     7201 tttctttttt cactgggaca aatttttcat ggacgaaaaa cagactcaac aagaagacga
     7261 aaaataagat gatccctgta atgaaaaagg tagggcgata gccaaacgct gaagcagaga
     7321 tcccgccaag aagcggtcca agtaatgttc ctgtaacaga accagttgct aaggttccaa
     7381 gaacttgacc acttttttct tttggcgtcc cagttgcgac aagggcagtg gcattactga
     7441 tataaccaga aaaaatccct tgaagcagac gcaatgcgac taattgatac acactagtca
     7501 cgcaacccat caaagaaatg acgattgcca ttcctaatga agcacgcaac agcatcaatt
     7561 ttcttccttt ttggtcggct aattttcccc accaaggaga gataagcgta gtcaccaaga
     7621 aagtagatga aaaagtcaat ccgctccaaa agttcaattg tgacgtacta taattaccta
     7681 acgtatctat gtaaagtgac ataaaaggca tgactaaact aaatccgata ccagccataa
     7741 atgttccaaa ccataagaca aacaagtttt gttcccaagt ttctcttttc ctaaataatt
     7801 tttcctttat tcttgcagcc aattagactc cttctttctg aaaattttca tctacagctc
     7861 tttattataa actacaatat gtagtttatc aatgaaagtc tcttttttct ctttcttttt
     7921 cgtggtaaac tgaaagagtg aaggatttat tttatgagag gaaacatgga aatgaaaaaa
     7981 agaaatctgt ctcaagagaa aatcatcgac tgctttcgag aactagcaga agagatggat
     8041 gttcagcaaa taacctttca gcatttagcc aaacgcttgg atatcaaggc tccctcgctg
     8101 tataaccatt tcaaaaatat tcgtgatgtc aaaacagcgc taacagcgaa acttctgcag
     8161 gaacttaatg accagctgcg acgggctctt gtgggaaaaa gtggtcagga agcactgcag
     8221 atctatgcag aaacttatcg atcctttgct ttttcaaatc aagcagtgta tgagttactg
     8281 atcagtgtac cgcataccaa tgaagctgtt ttattagatg gaatccatga aacgaatcag
     8341 atcattttgc agatatttga ggcttttcaa ttaacgaaag aagaaaaagt tcatcgaagc
     8401 agggaacttc gtagtatgat ccacggttat ctttccttac gttttttagg atactttaca
     8461 aaagaaccaa caatcgatcc agaagacagc tatttttgga tgatcaataa ctttatcgct
     8521 actttgcctt aaacaatcag attcatttgt aacaatttta agatgttcat taaatgaccc
     8581 ttttataaga gaaaattctt gcaactttct tgaaaagtga ctaagatgag tctagaaaag
     8641 ctgaaagaag gagcacgagt atgtatcaag taattaccat gttcggggac aatgagcctt
     8701 ggtggttttt tgaagactgg gaagaagata ttgaagaaga agaaacattc gagtctttcg
     8761 aagaagcacg acaagcgtac gaaaaacaat ggaacgaaat ccatcaaaat tatgaatata
     8821 tcaatgcaaa aagtaacttt ctcagcgcct tttggaatga cggagatgag cgctggtgtg
     8881 aagagtgtga cgatgattta cagcaataca agggacttgc gttgttgaaa aatcatcagc
     8941 ccatcacagt agaaagcaga aaggaatttt atgaaacaac taattctagc ggaaaaacca
     9001 agcgttgcga aagacctaag caaggtgctt ggtgctaatc aaaagcacaa aaattattat
     9061 gaaggaccca aggtgatcgt tacttgggcc cttggtcatt tattaggatt gaaaatgccg
     9121 gaagatctga ataaggaatg gcagagctgg caaatggaca cgttgccaat gatccctaaa
     9181 aaactaggaa tcaaaccact tcctaaaaca ggacatcaat taaaagcaat caagcagctg
     9241 gctcagcgaa aagatgtctc agaagcagtc attgcgacag atgccggaag agaaggggaa
     9301 cttgttgctc gctggatctt agagtatgtg catttcaata aaccagtgaa gcggttatgg
     9361 atctcttctc aaacagataa agcaatcaaa actggattca agcaactaag acctgcgaaa
     9421 gaatatgatg cattgtatga atctgctctt gcccgggcaa aagcggactg gctggtagga
     9481 ttgaatgtga ccagagcctt aactgtgaag tacgcggata atttatcagc aggccgtgtg
     9541 cagacaccga cactagcatt agtgagagac caagaaaaga aaatcgagca gttccgtcca
     9601 caaacgtatt tcacgatcac gttgcaagct ggaaaagaac aagccaaaat gcttcaaaaa
     9661 aatcaatttg ctttaaaaac acaagaagaa gcacaaaaac ttgttcaatt gatcagtaca
     9721 agcaaaggaa ctgtcacaga agtccaagaa aaattaaaaa cggaatctgc gccattgcct
     9781 tatgatttaa cagagatcca aagagaagcc aatcaacgct atggctattc tgcaaagaaa
     9841 acattaagtc ttgtacaaag tttgtatgag acccataaga tcgttactta tccgcgtaca
     9901 gacagcaaat atctgacaaa tgacatgaaa agtacgatga aagaacgatt gcaagcagtc
     9961 agcgactttg ctcctgaggt caagcagtat ttgaaaaacg gagccatcgt ccaacagaca
    10021 aaagtattcc aagacagtaa agtgaccgat caccatgcac ttttgccaac tgaaaacaga
    10081 gcacgttatg aaaaattaag taatgaagag cgaaaaatct atcaaatgat tgtctctcgt
    10141 tttttaggac tgtttgctca accccataaa gtcagccaaa caaaagttac ggttgaattt
    10201 ggtaaagaac gattcatttt tcggcaaaat cgtgtcgtcc aagcaggatg gaaaggcgaa
    10261 actgaaactg agacggaaac agtcaaatgg gaaaaaggaa tgagaatcaa tcccgatttc
    10321 gccatcaaaa aagaactatc tgcaccgcct aaaccattgt cagaagcaag cttgcttggt
    10381 caaatggaaa aatacagtct gggaacccca gctacgcgtg cagagattat cgagaaacta
    10441 atcaaatctg aactgatgga acgtacccct caaggattgc aagtatctcc taaaggtaag
    10501 caattattag tattggtaaa tccctcttta gtaacaccag atttgacaga aagctgggaa
    10561 aaagaccttg aagctattgc tgcaggaaag aaacaagcag gaaccttctt gaatggaatc
    10621 gaaaaagaaa caaaacgctt ggtgaatgag atcaaatcaa gcaaacaaga atatcaagat
    10681 ttctcgatta cccaaaagaa atgcccagaa tgtggggcca atctgcgcga gaaaaacaca
    10741 cgtgacggca aaatctatgt ctgcaccaat tcagattgca actaccgcag aagaaaagat
    10801 ccaaaagtct ctaatcatcg atgcccgcaa tgtcaccgga aaatggaaat catcgaaggt
    10861 aaaaacggtg cttatttccg ttgtaaatac gatggaacga cagaaaaatt gcttgataaa
    10921 aaagagcaga agaagaaaat gaccaaacat gaagaacgac gcttgctgaa aaaatatgca
    10981 caagcagaag aaccagaaga aagcccatta gcagcagcac taaaagcagc aatgaagaag
    11041 taataagata tgaaagaggt tgtgatagaa gtggtcagct tcaagaaata agaaggaatt
    11101 tacgaaaatt gcttctcaaa tttttgtgaa atttcggctt atttccgaag aagctacttc
    11161 tggaacacca tttattcgga aaaaaggact gtgacaagac ttttgtcaca gtcctttttc
    11221 atttaaaaaa atgtttactt catttgacgt atattattgg tgaggcttca attcatttgg
    11281 cttcactttt tctgtttctc ttatgatata aacaggacgc tgtttcgttt ctaagaagat
    11341 tttcccaata tatttcccga tgattccaag acataacaat tggattccac caatgaacag
    11401 gaagatcgag accatggaag gccatcctga tgtaggatca ccaaacaaca atgcccgcaa
    11461 aataatgaac aaaattgcaa taccagaacc gacacaagac aaagcgccga taaatgaagc
    11521 aatcgttaac ggtgcatccg agaagttcac gataccatca atcgagtagt tgaataattt
    11581 ccagaaggac caagatgttt cgccagccac acgttcgcgg ttttcataag aaatatattc
    11641 tgttttaaat ccgacccagc taaataatcc ttttgaaaat cggttgtatt ctgttaattc
    11701 taggacggca tccaccattt gacgagtcat caagcggaaa tcacgagcgc catcgaccat
    11761 ttctgtatcg ctgattttat tgatcaactg gtagaatttt ttcgcaaaga aactgcgtat
    11821 cgcaggctcg ccttcacgag tcgaacgccg ggtgccgaca caatcgatat cagaagtagt
    11881 gatccggttg atcatctcag gaagcagttc gggcggatct tgcagatcaa catccatcac
    11941 ggtcactaaa tctcctgtag aagcttgcaa tccagcgtac agagccgctt ctttcccaaa
    12001 atttctggaa aatgagacaa agcggacata aggataagct gaggctagct tcttgattgc
    12061 tgctaacgta ccgtcttttg atccgtcgtc aataaacaaa tattcgaact catgggcacc
    12121 tttgaatttt tcaacctcat caaaaaaaag aggaacagtt ttttcttcat taaaacatgg
    12181 gacaacaatt gatactttca tcgtttctat cctttcaatc gtatgtttat atatggttta
    12241 cattcaaaat ttaataaacc ttaaataaca cctttaaaat ttgattttac gtaaaagaac
    12301 aggtattctt tttgtaaact aacgtattta gtttaccatg tttcctcggt ttatcaaact
    12361 tttaagaaca atttttatta gttggcaagt agttttggat agagaaaaag gaggaagttc
    12421 cagtctattg actaggagca aacaaaatga aaatacacac acctatcggc tttttatggt
    12481 tagatgcaga tgaatccgga ttgactgctg tatcttttca gccaatttca gaaagcgaat
    12541 cagcgaatca gcgaatttta acgcgcacac aagaacagtt tgaagaatat tttgcgggaa
    12601 aaagaaaaga atttgatgtt cctttatcta taaagagagg tacgcctttt cagcaaaatg
    12661 tctggaaagc tttgtcgacc attccttatg gagaaatccg aacgtataaa gaagtagccg
    12721 ttgctgttga tagtccgaaa gctgtgcgag cgattgggca ggcaaatcga accaatccat
    12781 tgccaatcgt tattccttgt catcgagtga tcgggcaaaa tggaaaactg acaggatata
    12841 tgggaaacgc aatagatggg gttgaaatca aacgccaatt gttgttgtta gaaggatatc
    12901 tgcaaaatta aaaacagttg tgattcggac attgttccat gcgagaatca caactgtttt
    12961 ttcatgttca ttctaaactt tgatcagcca aataaatagt aaagccatca ctgtttggag
    13021 aagcccgaca gctaatccat aagataagaa gcgtgtacct tctttcatga attttgcaaa
    13081 gtctaaacgc agaccaattg ccgctaaagc agtgatctca aaccagctgc tgataaaatg
    13141 tgccgattgg tcaataacta caggtaaaga aaacaagctg ttgaccaaac aaaagatcac
    13201 aaaccctaat acataccaag gaagcggtaa ttttcgtttt ttctgcgtat cagttggcgt
    13261 ttcaaacaga gctggttgtt tcttgtgcat gaatttttca aacagataga caacaacaac
    13321 gagcatcata atacgtagaa tcttaaatag catggcgaat tcgacaacag aattgtcaag
    13381 taagcttgcc ccagcgacta cttgaccaac agattggagt gtaccaccca aaagtgcact
    13441 tttttcaatg agatttccac caaacaactg taatccaaga aaagggagag tcaacatcat
    13501 cacagtaccc agtagattga ctagtgtgat gatctgtcct ttttcttcct ctttcgcatg
    13561 gatggaaggg gcgattgaag caatagcaga agaaccacaa acagcgtttc ctccagccat
    13621 catcaaagaa gaagtatcgc tgaattgcag ttttttacca atcaagtaag caccgatgat
    13681 agtgactgac atttgcagta acacaaaaac aattcctttt acaccaattt gagcaatcgt
    13741 ttgagctgtg acagttgcac ctagtaaaaa aacagagaat tctaatagtt tgctttcagc
    13801 aaattttgtg ccgattgcta gaacaggctg tttaaaaaaa gtatttccta ataaaatacc
    13861 caataaaata gcaatagtcg ctccacctaa ctgtggcaga taaatagcaa gaagcctgct
    13921 gaaggcagcg atggcaaaag aaagcagtaa accgggtaag atttcgcgga ccgctgaatc
    13981 ctttacagat tttattggat gtgacaatga tttcatttga tcggttcctt tcgtcaataa
    14041 atactaaaag catactcgaa catagtagca ctaaaccagt attttgacaa aagaacaaga
    14101 ggtcgtgaca aaagtcttgt acgacctctt gtttcgaata
//
LOCUS       DACECZ010000067        14052 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.067, whole genome shotgun sequence.
ACCESSION   DACECZ010000067 DACECZ010000000
VERSION     DACECZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.067"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            227..1090
                     /locus_tag="IWO35_001786"
     CDS             227..1090
                     /locus_tag="IWO35_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0316845.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1219..2841)
                     /locus_tag="IWO35_001787"
     CDS             complement(1219..2841)
                     /locus_tag="IWO35_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0316846.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3090..3611
                     /locus_tag="IWO35_001788"
     CDS             3090..3611
                     /locus_tag="IWO35_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316847.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3797..5527)
                     /gene="spxB"
                     /locus_tag="IWO35_001789"
     CDS             complement(3797..5527)
                     /gene="spxB"
                     /locus_tag="IWO35_001789"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ0316848.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5692..6510
                     /locus_tag="IWO35_001790"
     CDS             5692..6510
                     /locus_tag="IWO35_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ0316849.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6613..6810)
                     /locus_tag="IWO35_001791"
     CDS             complement(6613..6810)
                     /locus_tag="IWO35_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316850.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6979..7557
                     /locus_tag="IWO35_001792"
     CDS             6979..7557
                     /locus_tag="IWO35_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0316851.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8602..9105)
                     /locus_tag="IWO35_001793"
     CDS             complement(8602..9105)
                     /locus_tag="IWO35_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0316852.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9464..10489
                     /locus_tag="IWO35_001794"
     CDS             9464..10489
                     /locus_tag="IWO35_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0316853.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10812..11690
                     /locus_tag="IWO35_001795"
     CDS             10812..11690
                     /locus_tag="IWO35_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316854.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12166..12282
                     /locus_tag="IWO35_001796"
     CDS             12166..12282
                     /locus_tag="IWO35_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0316855.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13210..13548
                     /locus_tag="IWO35_001797"
     CDS             13210..13548
                     /locus_tag="IWO35_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316856.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13642..13872
                     /locus_tag="IWO35_001798"
     CDS             13642..13872
                     /locus_tag="IWO35_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316857.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         4469 a         2325 c         2688 g         4570 t
ORIGIN      
        1 cacggtgttc cagaagtagc ttctacggaa ataagccgaa atttcacaaa aatttgagaa
       61 gcaattttcg taaattcctt cttatttctt gaagctgacc acttctgtca caacctctta
      121 ttttgattag aacttgcgaa aataaaccgg ttaaagtgtg tctagcgcaa gagatttgct
      181 ataatagacg ataagggtaa aatgtaacga gaaggggttg aaaaatgtgt atcaaattgt
      241 aacagattct tgttgtgatc tgccatacca tgtattagca aaagaaaaag ttgatttttt
      301 atcaatgaat ctccaattga atgatcaaga gttagtagat gacctcggaa agacattcaa
      361 ttatgaccgc tacatgaatc agctgaaaaa tggtgcgatg cctacgacat cacaaatcaa
      421 tgttggccga tatttagaat ttttccgttc ttatgcggaa aaacagattc ctgttttata
      481 tgtggctttt tccagtggat taagcggctc ttacagtagt gcgcttcaag cggttgaatt
      541 gctgaaggaa gaatatgaag accctgagat ctatgttttt gatacaaaag ctgcaagtat
      601 tggggaaggg cttttagtat tggaagctgt aagattgaaa aaagaaggta aaagcatcga
      661 agaaacccta gagtggctgg aaagtaatta tttgaaagtc cattcatggg taacagtaga
      721 cgatctgaaa tatctagaac gaggcggtag gatttccaaa gcaagtgcta ctcttggcag
      781 tctattgagt gtaaagccta ttattacggt ggataaagaa ggaaggctga cgaatgttga
      841 caaagttcga ggaagaaaca agtcgatcca gacaatcgtc aatgagacgg taaaaacaat
      901 cgttgcacca cttgatcaaa tcgtttatgt ggcttacgct ggggatctag agagcgcgga
      961 gaaggcaaaa cggcttttgg aagagcagat aaagatgaaa gatgtgaagc tttatccctt
     1021 aggaccaacg atcgccagtc acacaggcta tggctgtatc gcgatttttt cgatgggaat
     1081 cagtcgataa aagcagtaac tcattctgta aaaaagccca gaaattgatt gtaacaaaaa
     1141 cagcgactct taaaagatag atgagcatcc tatctcttag aggtcgctgt ttttttattt
     1201 tttaagctaa ataggccatt atgcccataa ctcgttcaat tgtttttgaa tatctgcatt
     1261 ttccaaaaat tcatcatatg tcgtatcagc tcgatcgatc acacctttat ccgaaacggc
     1321 aatgatgcgg tttgccaatg tttggataaa ttgatggtca tgtgacgcaa ataagattga
     1381 gccagtaaat gccatcaagc cgtcattcaa tgctgtgatt gattctaagt ctaaatggtt
     1441 cgttggatca tctaatacaa gtacgttagc tttcgaaagc atcatttttg aaagcatgca
     1501 acggactttc tctccaccag ataaaacatt cactggtttc aatacttctt caccagaaaa
     1561 caacattctt cctaagaagc ttcttaagaa ggtgttatca tcttcttctt tacttgcata
     1621 ttgacgcaac cagtctaaaa tcgttagatc agtatcgaat tctttcgttg tatcctttgg
     1681 taaataagaa ttacttgttg tcacacccca acgtactgtt ccagtatctg gcgtaatttc
     1741 acccatgata actttgaata gcgttgttgt tgttatatca ttttctgcga cgaaagcgac
     1801 cttgtcttct ttgctcaaat taaaagatat atcatctaaa attttctttc catcaatcgt
     1861 tacacttaca ttttctacct gcaataagtc gtttccaatc tcacgttcag gtttgaatcc
     1921 aacgaatgga taacgtctag aagaaggttg gatatcgtct aaagtaattt tttcaagcat
     1981 ttttttacgt gaagttgctt gttttgattt tgatgcattc gcactaaagc gggcaataaa
     2041 ttcttggagt tctttgattt gttcttcttt tttggcattt gcgtttgctt gaagtttagc
     2101 cgctagttga ctagattcca accaaaaatc ataatttcct acataaagtt tgattttacc
     2161 aaaatctaga tccgccatat gtgtacatac tttgttcaaa aagtgacggt catgggatac
     2221 aacgatcaca gtattttcaa aattgatcaa aaattcttcc agccaattga tcgattggat
     2281 atccagaccg tttgttggtt cgtctagcaa aagaacatca ggtttaccaa atagtgtttg
     2341 tgctaataac acttttacct tttgtccacc agttaattca ctcatttgct gatggtgcaa
     2401 ttcttcaggg atgttcagac cttgcagtaa aacagctgct tctggttcag cttcccatcc
     2461 attaagctca gcaaattctc cttctaattc agctgctcga ataccatctt cgtcagtgaa
     2521 atcctctttc atgtaaatcg catctttttc tttcagcact tcatacagac gcttgtgtcc
     2581 catgatgact gtttcaagta cagtatagtc ttcatagtcg taatggttct gtttcaatac
     2641 ggccatccgt tcatctggtc ccatagtaac ggaacctgtg gaaggctgca gatcccctga
     2701 taacacttta aggaacgttg atttacctgc accatttgca ccaatcaagc catagcagtt
     2761 tccgggggta aatttaatat tcacatcatc aaatagtttg cggtctgaaa agtgcaaact
     2821 cacatcattt acagtaatca atttttttcc tcacttttat cattattagt tttgacaagt
     2881 cttatagaat tatatagggg aaaggtaaaa gattcaaggc aatcgaacat tctttgtgtt
     2941 tcacgtgaaa cagaatgaaa aaaataaaaa taaaagcgga gcgaaaatag ctactatagg
     3001 atttattagt ttgcaacagc ataatttttg acgttattta taagagggag tactataaaa
     3061 ctgtaaaaga aatgaaagaa ggattgaaaa tggatgaaaa aagaaccatc gactggggct
     3121 cattgatttt gggaatttta tttgtacttg tctcattgtt atcatttcaa gaccctgtcg
     3181 gaaatttagt tgcaattgta gtagtctttg cgatttttgc ctttattaaa gggatatttg
     3241 aactgtttgt acggaacaga ttaaaagagc taacaggata taaaggaaag atgcccttaa
     3301 taatcgggat catcgatatt ctagttggcg tctttttctt attcaatatt ggtgcaggag
     3361 tcgcagcatt gccttttgtt tttgcagtat ggtttattgc tgattcagtt ttagcgttat
     3421 ttactgctga cttggcaaga ggagtaagtg aaggatatta ttggtttacg atcatcgtca
     3481 atattttagg aattttatta ggaattttct tattgtttaa tccaatttct tctgcgttga
     3541 cattaagctt tttagttgga ttctatttta tgttatttgg tatcacacat atcgtctatg
     3601 catttagata agtaaaatca aacaaataaa aaagccggct tccgattaca gttttactgt
     3661 tgtaattgga agccggcttt tttatcatgg acaaatatgc acgaggtgat ttttaggtgt
     3721 cgagacaaat ttatgcaaaa atgtgagaag ctatatacac aaattttatc ttaatactcg
     3781 aagcatagcg cagttgtcac aacctctttt catataattc attcaatact ggcaaatcat
     3841 ggacttcata tttttctttg aatgcttgga tttcttcttc agaatatttt cttggatcta
     3901 aatgcagatc ttctactggc aatgggcgct tgttattgat cttcacatcg attactacag
     3961 gacgatctga ttttgcagct gcatcaaagg caaatcgaag ttgcgagtat tctgtgactg
     4021 taaagctatt tgccccgagc gcttctccaa ctttaccgaa atctgcatct tctaaaaata
     4081 caccaaattt cttttgttcc gtatcttctt gttctgcttc gatgaaacca aaagaatcat
     4141 tcgaaaagac aacattaata attggaagct gatatttgac ttgtgtaatg atatcttgca
     4201 tgaccatagc aaacccgcca tcaccactta aggtaaatac ttgtttttct gggtagctta
     4261 gttgtgcggc aattcctcct ggcacaccat tacccatagt tgcgaaccat ccagatgtcg
     4321 tatgtttttg tttaccgttc atattcaata aacgaataga atggatagtc gtgttaccga
     4381 catctgtgac gaaaatcgca tcgtcttcag caatacgatt gatttcttta tagacagcct
     4441 ctggacgaat cggttcttcc tctcgatctt caaaagattt caaccaattt acccattttt
     4501 ctttattttc ttgatttgct aggtaccagc tatcagcagg gcgagcatcc cctaactcga
     4561 caagtctttg caaagctgtg tttgcatcac cgaggatcga caaagatgtt tcatggcgtc
     4621 gtccaaattt tgacgcatcg atatcgattt ggataaattc agcagttgga ttaaaaaatg
     4681 cacgtccaaa tgggaagtcg ctaccgacaa ataagatcag atcggcttct gccaatgctt
     4741 catttccggg tttagtagcg acacgtccag caaaaccaag aaagttttca taatcatcag
     4801 gaataattcc ttttgctaga acggaagcga caacaggaac tgaaaaatgt tctgagaatt
     4861 ctttgatttg cggaaatccg tttcgcgttc cttgaccaac ataaagaact ggtttttttg
     4921 ctttttcaag ataaggcaaa gcagctagca aatctttttc ttctggtaat atcacacttg
     4981 ttttgtgagt gtgagcggtg gagaaaggtt gttcgtcaat ttcttcaaaa ccaagatcaa
     5041 cagggatagt cacgatagcc acgcctttat gctcataggc ggctttaatg gcttcatcta
     5101 ctacatgcgg aagactttgt ggtgtcatga ctgtacgatt gtatacgcta acatctgcga
     5161 aaataggatt ttcatttagt tcctggaaag agttatagtt catggatgta gaagcaactt
     5221 gtcctaagaa cgctactaga ggcacatggt ccatctgtgc atcatacaaa ccattgatca
     5281 aatgtgtcgc accaggtcct gcagagccga agacagcgcc gactttcccc gttaattttg
     5341 catctgcagc ggctgcgagt gcgccgactt cttcatgacg aacttgaatg tagttgattt
     5401 cggttttttc ttgatatagc gcatccataa ttgaattgaa agatcctccg ggaatgccgt
     5461 aaatatggtc gataccccag ctttcaagaa cttttaccat tgcgattcct gcattgattt
     5521 ttgacatggt gtttcactcc tttagcaatg atgatacata aattttttgt atctagagta
     5581 tagaactttt tatcgaaaat cccaactaat tcgtcttttc acaaaaatgt agtcatgttt
     5641 ttgggaatat gctagagtag tacatagata gattcaagag aggggaatgg catggttgaa
     5701 tggctattga gacttgaacc ttggcagcaa gcacttgctg ggacagcttt tacttatttt
     5761 atgacagctc ttggtgccgg gttggttttt ttctttaaag aaatcaaaaa agaagtgcta
     5821 aacttgatgt taggatttgc ttctggtgtc atgattgctg caagtttttg gtctttgtta
     5881 gatccggcta ttacaaaagc agaagaaaat ggagatattg cttggctagt tgtaagtatt
     5941 gggtttggtt taggcggtgt gtttttatat atggcagata aaacactgcc ccatatgcat
     6001 tttggaccac aacatgaagc agaaggattg cctacccatt taaaacggac gatcttgctt
     6061 gttttttcta ttacattgca taacattcct gaaggtttag ccgtgggggt agcattcggt
     6121 gcagcagcaa ctgcggataa tccgacagcc gctatattag ccgctgtttc ggtggcgcta
     6181 ggtattggta tccaaaactt cccagaagga gcagctgtat caatcccgtt gcgacaagaa
     6241 ggtctgagtc gtaaaaaggc ctttgtttac ggacaagctt ctggaatcgt tgaaccaatt
     6301 gctggagtga ttggagcgct actagtaacg aaagtggagc tgctgcttcc ttatgcactt
     6361 gcttttgccg caggagcaat gatttatgtc gtggtggaag aattgatccc tgaagcacag
     6421 cagacattgt ccagcaaacg tcattttgca gtttttggcg tcatgagtgg atttatcatt
     6481 atgatgattt tggatgtggc attaggataa ataaaaaaaa tatatatgat atatcaaata
     6541 gggccgcgaa cttatatcgc gaccctattt ttttagtaag ttatatcata atgaagtgaa
     6601 tggtatcata cattaataat agttttttct atgtgtattt cctttgatcc agcgaacaag
     6661 ccagatgata agtgcgattg ggattagaac tttgaccgca aaccataaaa gcccgcttag
     6721 gacactgaca aaaagactga tgacgaagct agctgcaatt agcactaaaa gaaccatgaa
     6781 gatatttaag aacatatttt tttgacgcat acaaaaccct cctcttgttc attctttact
     6841 atagaaaaag tataacaggg attgtttaaa aacacctcca actgaagact gatttttact
     6901 tcatctactt aaatgatcaa acaattgctt tttctctggt ttatcatcta aaatagagcg
     6961 gaaaaggagg agaatatcat gcctaaaaat cgtcttgaag catttacaga tgctgtaatc
     7021 gcgattatca tgactatttt agttttagaa cttcatgaac ctaacggaga cacgtgggag
     7081 gcgctcgctg cattaggtta ccgatttttt atctatattg tcagttttat cattttagcg
     7141 atttattgga acaatcatca tcatttattt caagttgtac ataagataaa tggacgggtc
     7201 ttatgggcga acagtttctt tgtttttgcg ctttcgcttt ttccgtttgc aacttcatgg
     7261 atcagtgagt acatcaatag cctggctcca gaaatgactt atggttttgt taccttgacc
     7321 gctaatattg cgtattatat cttggcaaaa gaacttgtca aagcaaataa aggccacgag
     7381 aaagcattga aggcgatgtt gaataattac aagaaatcgt atctatcgat tggtttgaat
     7441 gtgttaggaa ttgtcttagg gtatttagta gcgccagtag ccgtgctgat cgcaaacatc
     7501 gtgattctag ctgcttggat catcccaagt cgtaccatcg aatcacaata tcggtaaaat
     7561 gatccgtgtt taaaatttaa gagaaaaata tagaaaaaat aaataaacga tcaaatatcc
     7621 gagtgatact agtattcaaa gagggatgca gatggatcaa aaaagaattc atgtaggact
     7681 gattactagt tttgtcacga tgcttattta tttggatcaa ggtcatttga cttggacagt
     7741 agttggtgtc gtaggatttt tgacattatt cacagcaata aaaaaataaa catctttatg
     7801 taatctgatg ttctatctat ttaagaaaag catgtttaca gcagtatgat tctgttgtaa
     7861 acgtgttttt atttgtggaa aatttaattt ttagaaaagt gtatcgaaaa atagatagtt
     7921 agtttttcca cagtggataa cttcgtaaga gataaaaata aaaaattagt caaagaaggg
     7981 cgtatagatt tcttttgata ggagatatac aacagaaatg aataaatatt aacgtgtttt
     8041 caaacagtat aaaacggtgt ggtttttgga ataaaaaaat aacatatctc attttacact
     8101 tttgttattt tatacttaaa aaataacgac acagaaaata ccgtgtctca gatttctcta
     8161 ctacttctaa aaactactgt ttttttcctt tgaaatgcct tcaaaaagta ggatcaggca
     8221 gaatatactg gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac catagaaaat
     8281 ataaagtaat tacttataaa acaagctttt tatagaaaaa taatatacct gagtagggta
     8341 taaattaaga cggaattcct ttctagattg agatattgac taggaagaat gttaagattt
     8401 taagccttta agctaaaaaa aaaccctttt tgttaaataa ggggaatata gcctgtcagt
     8461 ttcaaactgc aagtcgtcct gctgcaataa tatagtagat tagagatgaa aatagatttt
     8521 tttaaaccaa tgactgaata aaaagaccgt catagataca cattggaatg tgtctattac
     8581 ggtctttttt tacgaacgat tttattgaag acggacagtt cgggtcaaaa ataaaaaagc
     8641 tacgatcaga aataagaata aaatgtatcc caaaataatg gctagacggt ctaacttatt
     8701 tgtcgataga tgtttgttta gaagttgata ggcccataaa cctatcagtc ctcctgttgt
     8761 attagttatc aaatcagtga tgtccgctac gccaataccg aataggaatt gaatcaattc
     8821 tgccaagaag cttacaagga aaataatccc taatttttta gaaaaagaaa gtctcttgaa
     8881 attgacattc aaaagcaccc caaaaggaaa aaagacaatc aagttataag tggtctcacg
     8941 taataccgtc gtattttcaa aagaaaaagt agaaaaaggg acgagattga cattgctgta
     9001 agaataagtg agaaattgtg gaagattaaa tgatagcttg aataaaatca accataatag
     9061 aaagaataaa taaatcccta ataaaaattt tgatacatac cgcatagaca tctcctccat
     9121 ttaaaaagta taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa gtttaaggac
     9181 caacgtagtt atcgtacgaa aattagaagc ttttttttgt tgggaatccg gtttctttaa
     9241 caaagctaag atagcgagtt gcttggaaaa gttgctatct tttttctgtc aaaacgactc
     9301 ggaatgttcg ttttttgttc taatttctcc aagttgttta ctttttgata ctaagtttat
     9361 gaaaaatcaa gaaatagttg ttatactagg taatgctgac aaacgggatt ggcatttcat
     9421 cttggttcag ctttttttcc aattactagg aaggagggat agattgttca agacttattt
     9481 tcgtttcaat aaaatacaag ataatccttt tcgtatcatg aatctgatgc tacttatctt
     9541 tattgtatta tggttaggtt acataaatca tgattttttg atttcaagtt tttcatcatt
     9601 agggatcttt acttttttct attatcaaaa cataccgatg aaatatttgc tgaaacgtat
     9661 gtttctgatc ggttgcggac tgttggttgc tttcgttttg gggatcttat cgacttatgt
     9721 tttatggctt gaaccatttg ccgtagcttt ggttgctttt tccagcagat tcgtgttgcg
     9781 attattccat atttctaaac caggtggctt gttttttgcg atgctttctg ccatgggaac
     9841 gagcatgcag ctgccaatcg ctcaattacc gatcgtctca ctttattttt ttatgggtgt
     9901 cgtttttgcc ctgattgcgg cagtgattac aaaattactg gattcacggc ctgagcagac
     9961 aattgaaaaa gcaactttaa aagaaagatt tcacgaagag cctttagtga tcattgattc
    10021 tgttttctac agcgctgcct tatttctttc cgtttatgtc agtcacggct tgaatctgca
    10081 taatccttat tggttgacct tgtcatgtgc atcgattttg ctagcagaaa acttggatgc
    10141 gatgaagcat cgacaagtcc aatatctgat tggatcgatg ggtgggttat gtgtgtcggc
    10201 attcttgtca tttgtgccgt ttacgcagtt gcagaccatt tttctgatca cttttctata
    10261 tggaatcgca caatttctag ttgctagaaa ttatgcagtt gctaatattt ttcttaatcc
    10321 gatggcgttg atgctatcta cacttattcg tggagcatac ttgatttctt tgatcgaata
    10381 tcgtttttta gggatcgtca taggaagttt catcggttta ggtgttgcgt gggtaatgac
    10441 ggttggtttg caacattatt taacggttgt tcgtcaaaaa ttagaataga aggacaaata
    10501 tatgtgccaa aagaggttat gacaagattt ttgtcacaac ctctaattca aggtgttcaa
    10561 aagccagatt ctcagcatta agtcaaattc acacaaaaat gtggaaagga actttcaaaa
    10621 aaattttata tttattctat caaaaaattt tgtttatgat ggtaactgaa taaactttcc
    10681 tgagatggtc aatgctttcc ttgaaagtct tacagatatt ctttcttcat agaaagttta
    10741 agttttatag ttagtgtaca ataaaagtat gtttatacca tatggtgtcc ggataatcag
    10801 tcatttaaac aatgaggttc aaagatatga aaaggggaaa tttgatgaag aaaataagca
    10861 atgaaatcca aattctggca gaaactttaa ctcttcccaa agtcgttata gagcaaatga
    10921 ttgtacaagc aaaaaacatc cttgatgagg tgtttgagca atatagtcat gaactaatgg
    10981 atcctaataa agcaaaggcg actgcaaaga aaatagacaa agtctatgaa gaggaacctt
    11041 ttgcagcatt agctatttat ctatatgcag cttctcaaag ttggaaaaaa atttatcaac
    11101 cattgaagat tccaagaact atctatttgg caacaatgaa tgcttttact agatttattc
    11161 atgaacattt ccaagtaaca ggaagctatc gctttgaccg tggtttttgg atttggcatt
    11221 acactagtgg cttgatcttt cggattggtg aattggagta cgaaatgaca tattttccga
    11281 aagggtacac ggatctaaga ttagaaggaa agcagtgcct ttctcttcat attccttccg
    11341 atgcagattt atctcagaat aagataagag atagttacga aagtgccatt cgtttttttg
    11401 aactttattt tcctgattac cattaccaag tgatgtacac agatacttgg ttactatcac
    11461 ccaatcttac taaatggttg aaaaaagagt caaaaatctg tctttttgca gccgattatc
    11521 gattattatc agttgatgaa caggacgata gtggagtccc ttggatcttt ggacgagtag
    11581 atgcccaaat acatgattat ccagaaagta cgagtttaca gcgacaagca aaagagcaat
    11641 tgcttgcagg cgagcacatt ggctctggct taggtattct tacttcataa gtttagtact
    11701 tcgaaatagt ttaaaagagc gtgagacaaa agtaaagaac acttttgtct cacgctctaa
    11761 acacgtataa acggcggaag cagaagcaac tccttcggaa ataagctgaa attcacaaaa
    11821 atttgaaaag caattttcgt gaatttcttc ttatttcttg gagttaaacg cttctgtccc
    11881 agcctctttt ttagtgttct atcgaacaca cagtacgttc aataggtaaa ctagctataa
    11941 caatagatat taaagttaac ggacaagagg gcctttttta ggtagctaaa acgtaataat
    12001 gaaaatttat ttccagagat aggagtcaat cagctagtct gatcgactgt tttaacaagt
    12061 tgactttcat tttaactttt gataccattt aaaacacaaa tcgtaattgt ttcgatttgt
    12121 gttttttagc atgactacaa tcaaagaaga aagtgggtcg tccatatgga aaaaacagat
    12181 atctcaagtg cataccgccg cttgaaaagc ccaaatatca aaacacgaaa acgtgcgctg
    12241 aagatcatca aagatgtgaa acggaatagt gggaaaaggt aagaagaagg aaacttaatt
    12301 aaaaaaatag ccgaaaaacg tttttcggct attttcaata atcggttttt gtgtaaggtt
    12361 tccgaaagaa ctgctgttga ttttccattt ttaacatatg ataaattggt agaatctttc
    12421 ttttcaaatt gataccattc cctaattcca gttatatagt taactacaga accacaaagc
    12481 acgtacgttt ataaaagtat tatctgtagt tatttttcaa ttccctttca acctcttatt
    12541 ttgaccagta gtattgatca ctttaccttt taacaccttt gttcgttttt gaaacatttt
    12601 tagtcaagaa tggaaacgta actacataat caaaggtttt gcgaaatatt tcatgaaata
    12661 tttcatgtag aagcgagcaa tacggttgca aataaccatt atcgtttaat aagcagttac
    12721 gaaagctccc aatatctcta acgttcagct cactccattt cttttcttca aaaaaatcag
    12781 tgattcgtat ctctttctat ttgtcgaaaa agttaacttt aaaaagtaac gccgaaaaaa
    12841 tatgcaaaaa aataaaaaag cagttactat tgctagaata acatcaagga ataataagtt
    12901 ttggttttca gataatagtc gataagttgt gtattggaat tttagataca gtaataaagt
    12961 atttttcatg aaattttgtg agaatcacta ttacaagtat ctttaataaa attaatggtt
    13021 aaggaggaac ttaaagtaga atgtaaatga atagttgtag tcagtcgatt gtgatactag
    13081 actcaaagtt gcaggaataa gtttttcaat ttgtgattgg aagataaaaa atgaaaaaga
    13141 agggcttttt tttatacttt gttgattgta gtgtttattt tccctctgtt tagccaagta
    13201 acattttcta tgactcaacc agtttcaagt gaggaacatg ataacctttt tcgtgctaca
    13261 gaagagttta agaaatatca ggaaaatatt gttccggaga ctccattagc tagtgagcca
    13321 attactgata ttgatggaaa ccaaatagga tggtatatac agtacgaagt tatggtggta
    13381 aataatgact caatagtaaa aaatacagta ggcttagagc taacaccgat tgaaaaggtg
    13441 agttcattgt taacgtatat ttatactgat gaaagtgacg agactgagat cattttaacg
    13501 gattactcaa aaattgctac tgataaaaag tatatattgt ggatgtagac actagtgaaa
    13561 tactttatga tatagaaatt gatgaaaatt tactagatgt agcacaagaa atcgatcatc
    13621 aaaagcagga agctattcga tatgctggta gaaaagggaa aagaaaatat ttattacgtg
    13681 aatgtagcaa aagttagaga agatgaaaat gagtggaaag aattcaagag tcgctactca
    13741 attaactcga ctccgacatt tacagtttac agagaaggat cgatagaaaa aactgttttt
    13801 tggactaaag aaagtggaat gtctttagct gaagtagaag agtttttgga ttatgttagt
    13861 atgcaacagt aagggagttc ttattatgaa aaattttgac acatattatg ttattgtttt
    13921 ttcgatatct ttgctgttaa tattactaac tttaatgcag tataaagagt atccagagta
    13981 ttttatttta acttttgtat atttagtaag tagctacaca cttctatata aattcagaag
    14041 ttcttctaga gt
//
LOCUS       DACECZ010000068        14037 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.068, whole genome shotgun sequence.
ACCESSION   DACECZ010000068 DACECZ010000000
VERSION     DACECZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.068"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IWO35_001799"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IWO35_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ0316858.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IWO35_001800"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IWO35_001800"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ0316859.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IWO35_001801"
     CDS             complement(2169..2906)
                     /locus_tag="IWO35_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0316860.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IWO35_001802"
     CDS             complement(2997..3524)
                     /locus_tag="IWO35_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ0316861.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IWO35_001803"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IWO35_001803"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ0316862.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IWO35_001804"
     CDS             complement(4401..4886)
                     /locus_tag="IWO35_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ0316863.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IWO35_001805"
     CDS             complement(5039..5236)
                     /locus_tag="IWO35_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316864.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IWO35_001806"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IWO35_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ0316865.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IWO35_001807"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IWO35_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ0316866.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IWO35_001808"
     CDS             9211..9423
                     /locus_tag="IWO35_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ0316867.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IWO35_001809"
     CDS             9425..11104
                     /locus_tag="IWO35_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0316868.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="IWO35_001810"
     CDS             complement(11358..11558)
                     /locus_tag="IWO35_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0316869.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="IWO35_001811"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="IWO35_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(12249..12322)
                     /locus_tag="IWO35_001812"
     tRNA            complement(12249..12322)
                     /locus_tag="IWO35_001812"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12286..12288),aa:Ile,seq:cat)
     gene            complement(12634..13032)
                     /locus_tag="IWO35_001813"
     CDS             complement(12634..13032)
                     /locus_tag="IWO35_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316870.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
     gene            complement(13025..13477)
                     /locus_tag="IWO35_001814"
     CDS             complement(13025..13477)
                     /locus_tag="IWO35_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316871.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            complement(13464..13871)
                     /locus_tag="IWO35_001815"
     CDS             complement(13464..13871)
                     /locus_tag="IWO35_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316872.1"
                     /translation="MSFLLLKYLEIYSVKKNKLIGENDSPIKTPIELSLRNRMGILQR
                     SEIVKELFYHKFSYEIRLDDTYEHYRIVFFVYNSIEDATATTALTFGFTKNGTINSDK
                     TRQAATESDDICKKVCNGEENCWIGEEKLNEIY"
BASE COUNT         4145 a         2945 c         2362 g         4585 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgatatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaattatg taccctgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc
    12301 tgaccaacta agctaagggt actggttgtt gccacataaa gccataaaca atcaaccagt
    12361 agaatgtgtg gcaacaaacc tgttatcgca tagcttggag tgtgactatt atgggtgata
    12421 gtgaagatat gcgataacat cactatttta tcgaatgatt tttatagttg tcaatacagt
    12481 catctaaata cttcatatca atcatttatt cagaaacaaa ctatcttgca ggatctatac
    12541 gagttaatta cagagcaaaa aagcctcaac caaggatgac tggttgaggt ctttgtcttg
    12601 caaatttaaa tagataactc tattacttgt attttatttt tgttgactat atagctgata
    12661 taaatcaata actgtaggca taactcctac ttcgctatat aaattagaaa tcaaattaga
    12721 agctttattc aggataggtt tagctagctc tcccatagat tcttctacta attttttata
    12781 tggctgttca tttgtagaaa cacgaataac tgaaaagagt aaaatatcta ccggtgctcc
    12841 ttccatcccc cctacaatgt gagctcgtaa agaaacaagt tttttttcta gatactctgc
    12901 ttcgtcttct ttatatccaa gttcaatatt aaactctaaa tctccataac taaaattatc
    12961 agttaattca aaatgaattt tattcaaact aatattttca agtgcaaaag taacactatt
    13021 tcctttatcc atgtacatat acctcttttt ctacaatata gcggctcgta tcttggtaat
    13081 taacactagc atagaccata tttgacgata cttcttttct ctttctactt ttaaatactg
    13141 agtaactttt attccgaata taaggtttat tttcaatacg gtcatattta atagggccgc
    13201 cagcaacctt aatagtgaaa ttcattgaat ctttatagga caaattttca acttgtttca
    13261 aaagtttatt gtactctgca acaggaatat gcatatctcc gaactcaaat tcaagatatt
    13321 ttttttcact tattcctaac atttttgaga attctgtcac gtttttttgt ttttttaatc
    13381 ttattttaag taattgtttg gacaggtttc tttgttcaga atttaatttt ttatataaat
    13441 caggatgttt ttttaataaa tcattaatag atttcattca acttttcctc ccctatccag
    13501 caattctctt ccccattgca tactttctta catatatcat cactttctgt agcagcttga
    13561 cgggttttat ccgaattgat tgttccattt ttcgtaaaac cgaatgttaa tgcagtagtc
    13621 gcagtagcat cttctataga attgtatacg aaaaaaacaa ttctataatg ctcataagta
    13681 tcatctagtc ttatttcata actaaattta tgataaaaaa gttcttttac tatttccgat
    13741 ctttggagta ttcccatccg atttcttaag cttagctcaa taggtgtttt tattggagaa
    13801 tcattttcac caattaattt atttttttta acagagtaaa tttcaagata ttttaataga
    13861 aggaaagaca caattatggt ttaaatttga tccttcgaat agatttatca aagattttta
    13921 taaggtgtgg gattcagaag ttttctttct agcaatcgaa gatagcttat taatcaatct
    13981 ctactattct aataagaact actttaaaat ccctgctgca aaaactagaa tgaagaa
//
LOCUS       DACECZ010000069        13999 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.069, whole genome shotgun sequence.
ACCESSION   DACECZ010000069 DACECZ010000000
VERSION     DACECZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.069"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..809
                     /locus_tag="IWO35_001816"
     CDS             <1..809
                     /locus_tag="IWO35_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0316873.1"
                     /translation="HLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELISTNVA
                     DKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGLKWSAI
                     DFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAKWQQDN
                     RILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRHSNASI
                     MLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            1383..1952
                     /locus_tag="IWO35_001817"
     CDS             1383..1952
                     /locus_tag="IWO35_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ0316874.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1939..2799
                     /locus_tag="IWO35_001818"
     CDS             1939..2799
                     /locus_tag="IWO35_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0316875.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2989..3693
                     /locus_tag="IWO35_001819"
     CDS             2989..3693
                     /locus_tag="IWO35_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0316876.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3698..5533
                     /gene="walK"
                     /locus_tag="IWO35_001820"
     CDS             3698..5533
                     /gene="walK"
                     /locus_tag="IWO35_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ0316877.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5530..6846
                     /locus_tag="IWO35_001821"
     CDS             5530..6846
                     /locus_tag="IWO35_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316878.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6847..7716
                     /locus_tag="IWO35_001822"
     CDS             6847..7716
                     /locus_tag="IWO35_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316879.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7771..8580
                     /locus_tag="IWO35_001823"
     CDS             7771..8580
                     /locus_tag="IWO35_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0316880.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8683..9312)
                     /locus_tag="IWO35_001824"
     CDS             complement(8683..9312)
                     /locus_tag="IWO35_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAQ0316881.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9482..10774
                     /locus_tag="IWO35_001825"
     CDS             9482..10774
                     /locus_tag="IWO35_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0316882.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10850..11512)
                     /locus_tag="IWO35_001826"
     CDS             complement(10850..11512)
                     /locus_tag="IWO35_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0316883.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11737..12021
                     /locus_tag="IWO35_001827"
     CDS             11737..12021
                     /locus_tag="IWO35_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ0316884.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12072..13697
                     /gene="groL"
                     /locus_tag="IWO35_001828"
     CDS             12072..13697
                     /gene="groL"
                     /locus_tag="IWO35_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ0316885.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4704 a         2366 c         2974 g         3955 t
ORIGIN      
        1 atcatttaca agagttttat caatatatgt taaatgataa ctgtacagca aatacggtta
       61 ttcattatca cgcatttata cgcaaagctc ttcaagaggc tgttattaca gaattgataa
      121 gtacaaatgt agcagataaa gtaagaaagc caaaaaagaa acaatttgtt tctcaagttt
      181 ataatcaaca agagatcttg aaattacttg acattttgtc agaagaaaaa ctctatttgg
      241 tagttctttt aacagcattt tatggattgc gaagaagtga agttttagga ttaaaatggt
      301 ctgctattga ttttttaaac aaaaaaatca tgataaacca tgtaatcata gaaaatccag
      361 agaatatgtc acagttaata aagaaagatc aaacaaagaa ctcttccagc tatcgtacat
      421 tacctttggt tgaaacgatt gaacgagcat tagtaaatca agctaaatgg caacaagata
      481 atcgtattct tttaggagat gattatattt tagaagataa tgagtacgta tttactatgg
      541 aagatggacg gttgatgaag cctcaatatg taacgcatcg tttgagtaag ttgattaaga
      601 aaaatggatt gaaaaaaatt cgctttcatg acttgcgtca ttctaatgct tcaataatgt
      661 tagatagtgg ccagaatatg aaatccattc aagaatggct gggacatgca tcgtattcga
      721 caactgctaa tttatacacg catttaaccg caggagtaaa agatcgtgca gcagaagcat
      781 tagaaaacgc atttgggttt aaagaataaa aaaacaaaaa acaatatctt tggggtatcg
      841 cttcttgttt ttagaatttt tttgttggca atttgttggc agtatgttgg caatgaaaaa
      901 attcacaaac taaaaaccta tttataaagc tttataaaac ttttttcaca aatagagcat
      961 ttggttaaga gtcagctgct ctaccaactg agctaacggc ccgtacaaaa agtacttaat
     1021 aatagtagca ctaagaattt taaaatgcaa gactacattt aaagaaaatc agaaaaatct
     1081 ttacttcctt ataggaaatc attcatagat tattcacatt tgctcgctat tatataagca
     1141 taccaacaaa caaagttctt gcaagagaac aaaccctaac acttttttca ttttttactc
     1201 ctttcccgcc gtctccccgg cgggtctcct gttcttaacg ataaaagaca agaaggctta
     1261 gccttcttgt tttttgtttt catggaatcc cgctaatcaa atcacctagt taaagcaact
     1321 agattgaatg tttatcataa gtctgatatc atgtttatga aatgaaaaaa ggagcggtta
     1381 tgatggaaaa aatgcgtcag caattaatgg agtgggggaa ggaactagaa acattccgtc
     1441 tgcctcactg ggaagaactc cctgacttag aactttatat ggaccaagtc agaacgttag
     1501 ttgatcgtta tctttcacct gtgatacaag gagaaaagca tccattgctt acctcttcta
     1561 tggtaaacaa ttacgttaaa ctaggtttga taccagcacc agttaaaaaa agatataaca
     1621 aagaacacgt agcattttta cttgcaatca ctacgttgaa acaagtattg actattcctg
     1681 aaatcaaaga agggatattg ttccaaggaa aaacagtagg aattcgtgaa gcctataatc
     1741 tattttgcga tgaacaagaa gcagctgtgt ggatggtcag ccaattagca caaggtaaat
     1801 ctcatccgca aaaattcgac caggcaaccc ctgtagaata tattgctgta agagcggcaa
     1861 ctttatcgtt tgcaatgaaa cttcttgcgg aaaaaacgat tgttctagaa accgaatatt
     1921 taaaagagga gaaaacaaat gaaaaacaat aaaatcgcaa ttttagtaga ttcaggtacg
     1981 gatgtgccag aagaattgat aaaaaaatac gagatgtata tgatcccatt gaaaattatt
     2041 tataaagatc aggtatatac agataaaatt gacattacct ctgaagagat ctatcaacgt
     2101 ttgccagagg aaatccctgg tacatctttg ccggatggtg agtcgatcac agaaatcttt
     2161 gagcaaatca aaaaagacgg ttatgaaaag gtacttgcgg taacaatttc cagtggattg
     2221 agcgggacat ataatgtggt tcgtttaatt gcacaacagc aagaagaatt ggaagcatat
     2281 gtactagata cgaaaaatat tggtattggt gcaggttttt ctgcgatcca agcagcaaaa
     2341 tggctggaag aaggagtaga atgggatcaa ctgatcagta atctgaatga gttagtcgaa
     2401 agaacaaaag ttttctttaa tgttgcgaca ttggaatatc ttcaaaaagg tggacggatc
     2461 ggtttagttg cttctattgt aggaaccgct ttaaagctta acccaatcat ctcatgtaat
     2521 gaagaaggga tttattacac agtaggaaaa gctaggggac gtaaaaaaag tttggataaa
     2581 acggttgctt acgtcaaaga aaaaatcggt aatgcaaagg tcttcaactt agctgttgcc
     2641 catggggatg cgaaagaaga agcaattgaa atggaagcga gacttaaaag agagttccca
     2701 caagcaaacc aaatctactt tggccagatt tctccggcat tagtagtcca cactggtccg
     2761 ggattattgg gtgtcggtgt acaagtttta gaaggataaa tcaaaaaaag gaaacactga
     2821 ggtgaaaatc agtgttttcc ttttttattg aagttagaac aaaaaataat aaagtatttt
     2881 gcactttcat tttaattttc tcttttgttt tataggcaaa gatgaaacaa aagcagaaat
     2941 acggtataat gagaaggtat gacaataata tataagaggt ggctctttat gaaaaaagtt
     3001 ttagttgtcg atgatgagaa gccgatttcg gatatcgtca aattcaattt agccaaagaa
     3061 ggctatgatg tttatactgc atatgatggg gaagaagcct tggaaaaagt tgctgaggta
     3121 gaacctgatt tgattttact tgatttgatg ctacctaaaa tggacggctt agaagttgct
     3181 cgtgaggtac gcaaaactta tgatatgccg atcattatgg taacagccaa agattctgaa
     3241 attgataaag tcctaggctt agagttaggc gccgatgatt acgtgaccaa gccattttcc
     3301 aaccgagaat tagttgcacg tgtcaaagca aatcttcgtc gcggtgccac ggctgcgaag
     3361 gaaccagaag aggcagcacc tgcggaattg acgattggtg atttgacgat ccatccagaa
     3421 gcttacatgg tgacgaaacg aggagaaaca atcgagttaa cacatcgtga attcgaacta
     3481 ctcttttact tggcgaagca ccttggacaa gtgatgacaa gagaacattt attacaaacg
     3541 gtttggggtt atgattattt tggagatgta cgaacagtgg atgtaactgt acgtcgtttg
     3601 cgtgaaaaga tcgaagataa tccaagccat ccaaattatt tagtgacacg tcgtggtgtc
     3661 ggttactatt tgcgtaaccc agaacaggag taattggatg aaaagaaaag tccgcttttt
     3721 tcgatcagtc aattttaaga tcgctattac ctttattctg attttattga tctcgatcga
     3781 aattattggt gcctacttta tacgtggttt agaacgttcc acgatcaata catttattaa
     3841 agatatgaat caaacggtgg aatcattagc aacaacaatc agcccggaat tgaatcgaaa
     3901 agataatgca gatgatgaag aggtaaatgc gaatatcaaa agatttattg aaaacagtgc
     3961 gactagcgat atcatcgaaa tacgggtcgt tgacgaaaaa gggatcatcc gaggaacaac
     4021 cgatgtcaat gaacaaagcg ctgtgggaaa gaaaaatgat tatgttgata tcaatgattt
     4081 cacgactaaa cgttatgttg ctttagataa cgacgataaa cgggtgaata tcaatgttca
     4141 accgatcctg tccccgaccg gcgatgccgt tatcggggct ctttacgtca aaagtaatat
     4201 cgaacaaaaa tattcggaaa tcaataacac agcagtcatt ttctttactg cctcactaat
     4261 tgcggcattt atttcaatga tcgtatctgt tttagttgct cgttcgatta cacagccgat
     4321 cggtgaaatg agagaacaag ccattcgaat cgctagagga gactacagtc ggaaagtcaa
     4381 agtgcatgga caagatgaac ttggacagct ggctgatacc ttcaatcagt tagctgaacg
     4441 tattgaagaa gctcaagata caatggaagc tgaacgcaat cgtttggata gcgtgctatc
     4501 ccatatgaca gacggcgtca ttgcgacaga tcgcaggggt aaagtcatta cgatcaatga
     4561 tatggctgtt tccttgctag atatcaaaaa cgaggaagca atcggacaat cgattttgac
     4621 acttttagat atcgaagaag aatatacgtt gcggaaatta ttagaagatc caaacgaaat
     4681 ggtacttgac cgttcaacca gtacattgga aagtgatcag atgatcttgc gagtagactt
     4741 ttctatgatc cgtcgcgaat caggttttat cagcggatta gtagctgtct tgcatgatgt
     4801 gacagaacaa gaaaaaacag aacgggaacg tcgtgaattc gtctctaatg tttcccatga
     4861 acttcgtaca ccgttgacaa gtatgcgtag ttatatcgaa gcattgagcg aaggtgcgtg
     4921 gaaagacgag gaagtagcac caaatttttt gaaggtaacg ctagatgaaa cagaccgaat
     4981 gattcgaatg atcaatgacc tcttaaatct atctcgcatg gatacaggta atacccagct
     5041 tcaattagaa tatgtcaatt tcaacgagtt agtgaatttt gttcttgacc ggtttgatat
     5101 gatggtggga aatcaggaaa aaaattataa gatccgtcgt gaatttacgc aaagagatct
     5161 ttgggtcgaa gtagatacag ataaaatcat ccaagtagtc gataatatca tgaataacgc
     5221 gatcaagtat tcaccagatg ggggaacgat tacttgtcgc ttattagaaa cgcataataa
     5281 cgtggtttta agtatcaccg atcaaggatt ggggattcct aaaaaagatc tgaaccgagt
     5341 gttcgagcgg ttctatcgag tagataaagc acgtgcaaga gcacaaggtg ggacaggact
     5401 tggattagct atttctagag aagtgattaa ggcgcatcga ggcgctattt gggcagagag
     5461 caaggaagga aaagggtcga ctttttatat ctcgctgcca tatgaacctt atgaggagga
     5521 atggtgggaa tgagaatctc tgaaaaagtc gttcgtatag gactcatttt gatggttgcg
     5581 ttaagcatat acttctctta tgcaatctgg ctgagcccag caggaaaaag ttcaatcaac
     5641 gtagatgaga caaacactca aatgatcgag tctcaaagca atcgaaaaac ttccgaagtc
     5701 tttcttccgc ttcatgttac atggcaccat tcgggaatga tccaagaaac caacagtgaa
     5761 aatctcgtgt accgattgca attgatcatc gataaagcaa gatttggaaa gctttctgaa
     5821 gtagtttcag gagacaaatc acagtttgaa caggtgaaaa atctgaactc tggttttgag
     5881 ttggcctata atgcgccttt tttgctaaga gaatacaagg aagcatttga tttagattta
     5941 gatgtgtcga acttggcaaa agaaagcgaa aatatgtatt ttactagaat acaattcgat
     6001 caaggaaata aaaaaatccg atttttgaat tattcgaacc atctcgtata tgaagcaaat
     6061 atttcgttga attggacaga tatcaataaa gaattaacag caaaagacac aaaatggaca
     6121 gaaatgacaa cagaaccatc gacgatcgac acgcaatatg ctacgaaaca ttcgatcaaa
     6181 ctgaaaaagt atagttatat cctgtcacag caaccctata cactttttag aaatgctttt
     6241 ttccagaaac cagatagcgt gaaaaataat gatgaaagca atgaattgat tttttacggt
     6301 gggaatgaaa caatgtctat ccattcggaa acgcaagtag ttgattttcg tggagagctg
     6361 ccagagaaaa aagaaaatgc tagtgtattt tctgaaagtt tcccctatat tagcaaatta
     6421 ggcagttctt tagggaacct cagatatttt gaccgcagtg acaatacgat cgattatcgg
     6481 atattcgtgg aaggcttccc ggtttttggg acagacagca gaggacagat tggtgtggaa
     6541 gttggcggtg aaacgaatgg ttctgtgcaa gtgaatatcc aaaccagttt gaatacgatt
     6601 caagtgccga taccttcaga cgaagaagta gaacttgcaa gtacgaatga aatcatccag
     6661 cagttaaaag ctaagggagc agatagcaac aagattggtt cagtgatcgt tggctataca
     6721 tggcaaaatg tcaaagaaac gaatcgtgtc gttgatctct tacctgaatg gtatgtgaaa
     6781 tataaagata actggtattc agctaacgac cttctggaaa aattacctgg attggaggcg
     6841 aattaattgg atttcaaaaa aatcgaatgg atatttttcg tcgctttttt agggttgaat
     6901 gtgtttttat tcagcagtta tcatgacgcg gtataccaag aaaacaatgt cattcgctct
     6961 aatcagactg aatcaatcca acaacgtctg gctagcgacg atatcactta cacggggaag
     7021 atttcgtctg agaacaagca aggctattat cttagcgcgg agcagaccaa tcttagcact
     7081 tcattagcga attatcgcaa ggctggtaaa agcggcttgc tggcaagtgg tgtgttttta
     7141 gacggtgatg ttttgacgaa atctttatcc agttcagatg ctgaaaagaa cgtgatcgat
     7201 ccagataaaa ttagtagttc cttggaagat tttttatcta acaaagacaa tattttattc
     7261 ggtaatgatt atgtgtacac gaaaaacttt tctcatttag aagaagaacc actagaaatc
     7321 caagcaagcc aagagtatga aggcatccca ttcaatgatg atacagcaaa aatcgttctt
     7381 tctttagaaa aaaacgataa tgattatagc atcacgaaat acatgcagac gcatttatct
     7441 gatatcgagc agctgcgcga aaagacggaa ctgtatacgg aagaggaagc aatcaataca
     7501 ttgtacatca ataacaaaat accaagagga tcaaaaattt tatggcgtca gctagcttat
     7561 tcctgtattc tgaaagtccg ggaaaaaaat gtgtatgttc ctgtttggca tgtagcgatc
     7621 gaaacaagcg ataaagtggt ccaaattgag agtgtcaacg ctttcagcaa tacgatcgtg
     7681 acaaacaata ctgtaccaaa agtggaagat caataaaaaa gcgagtataa tgaaaaaagt
     7741 ttacaaagta agaagggaag taggagtcaa atggctgaag aaaaaccagc ttttaaaatc
     7801 agtatcctag caagtggaag ttcgggtaat tcactttata tcgaaagtga aaaaaaacgg
     7861 cttttggtcg atgcaggact tagcggtaaa aaaatcactt ccttgatggc tcaaattgat
     7921 cggacaccgg accaattaga tggaatactt gtgacgcatg aacatcgaga tcatatacat
     7981 ggcgtgggtg tcttggctcg taagtatcat ttggatgttt ttgccaatca gcagacttgg
     8041 gaagcgatgg aaccactgat tggaaatgta ccagtggaac aaaaacatct atttgagatg
     8101 gggaaagtca gaacattcgg cgatatcgat attgagagtt tcggcgtctc ccatgacgca
     8161 gcggctccgc aattttatcg tttccataaa gaaaatcggt cattcgtcat gttgacggac
     8221 accggatatt gcagtgatta tatgcgaggt gtcattgaaa atgccgatgg ttatttgatg
     8281 gaaagtaatc atgatcttga gatgttacgc atgggacctt atccgtggaa tttaaaacag
     8341 cgtattttag gagatagagg acatctttcc aacgaagacg gtgcacttgt catgactgat
     8401 gtgatcggta atcggaccaa acgaatctat cttggccatc ttagcaagga aaacaatatg
     8461 aaagaacttg ctcatttaac aatggaaaat gttttgaaag aaaaagattt tggcgttgga
     8521 catgattttg aaatctatga tacagatcca gatgaagcaa ctgagctatt ttcgatttaa
     8581 acagttcaat caataaaaat ataaaaagca gaagataaaa tcaatcaggg ttggttttat
     8641 cttctatttt tattttttta gcatattaaa aaaataaccg tattatcgac tgactacact
     8701 gatgtgctgc ttttcatgtt tctcatccag tatctcatct actaatgcaa tcgcgtaatc
     8761 tgccatgctg atcgtactgt cgccagcagc gtttctttgt aaataatcat cacttaacaa
     8821 ataagtacct gtacgtttgc catgtggatc aaataaagaa gctggactga tataagtcca
     8881 atgtagattt ttagattgtt tcaattttaa caatccttcg tacatattga aagctgttgg
     8941 atagaagggt gcatttttag gtacttggtc aatcatacga gtcgtctttt ctgtatctaa
     9001 gaataacgaa gaggcacccc caacgacaat taagcgtgta tcggttcctt caagcatctc
     9061 aatcaaatgg gacaaagaag tttgatgcat ttcttctttt cctttgggag cgttgaaagc
     9121 atcaacgaca acatcaaaag atgcaagatc agtagttgtc aactcaaata aatccttttg
     9181 tataaacgga acatctgatg gcaatttttc tggatgacgc acgattgccg tcacgtctaa
     9241 atctctggac aatgcttcgt ctaaaataaa tgaacctgca tggccagtag ctccaatgag
     9301 tgcaattttc ataaaaaacc tcctttttta ttcctgtcca ttgtaagcag ttgctgaata
     9361 ttcgtcaaag aatccgattt ttgaaaagct cagtgcgcat tcctttcatg gttcgtgatc
     9421 attgggtata gttaaattaa gaaaactgat tgataaaaat agaagttgaa ggagataaga
     9481 aatgaaagag tggttagatc gtgccttttt accaatgata acatctgttg tgaatcttag
     9541 acccttaact gctttgcgta atggaatgac ttacacgatc ccaataatta ttgttggctc
     9601 gctgtttttc ttatttgctc attttcctgt ggattacatc gctcagtgga tagacaaaac
     9661 aggattttct atgtatttta cacaagtata ccaggctaca tttgatgtga tagccttttg
     9721 gatcgttttt gccattgcat acgcttatgc aaaagaagaa gacatcgatc gcttaccagt
     9781 cggcataaca gccttgtctg cttatctact gcttattcag ccactaaata ctcagataga
     9841 tactgtctgg acaggtgctg aagggatact aggagcagtt acaactgggc tcatcatcgg
     9901 atggggctat gcacgtttag taagacttgc gaaagggtgg aagcaaagag agaagatacc
     9961 tgaaaatgtt tggcagtctt ttacttcatt gattccaatc attgttatag tcacttttac
    10021 gcttatcggt tcagcggttt ttcaacagtt gttcagaatg actgttgttc aaggagtatg
    10081 gacatatgtc cagcttccca ttcaatcgtt catagacacg ttgtttggtg tgcttttatt
    10141 aggttttttt gttccgtttt tatggttatt tggtcttcat ggatctgcga tggtcaatgg
    10201 gttggttagt cccgtcttgc aagcgaattc attagcgaat gcagaaatct tagcctcagg
    10261 acaagagttg acggtggcaa atggcggaca tatcgttact cagcaatttc tcgatcagtt
    10321 catgacggtt acgggtgctg gattgacgtt aggggctgta ttttttatga cggtttttgc
    10381 gaaatcgaag aaatacagag aattaggaaa tcttgctctt ttaccagcct ttttcaacat
    10441 caatgaatca attatttttt ccacaccgat cgtgatgaac ccgatgatgg ctgttccttt
    10501 tattttcgca ccggtcgttt caggattgat tacctattcc gctttatatt ttggattcgt
    10561 tcctttattt acagcagtcc aagtcccttg gacaacaccg ccgatcctct cgggtttctt
    10621 agtaggcgga gtgcctgcag cgattttaca aggtatcgtg ttgtcaatta gttttttcat
    10681 ttatttccca tttttgaaaa aaatagattc tgcaaactgc aaaaaggaaa gacagacaaa
    10741 aaatgatgga gcagcagaaa agatgaacga ctaaaaaaca aaaatactcc tgtcgcttta
    10801 gattgggaaa atcaccaatc taggatgaca ggagtattta tagtcatgtt tatactagcc
    10861 ctttttggac agctaattga acaagaacaa ctaatgtgtt cattcctacg tgtgtgatca
    10921 tcgatgtcca gatccgacca gttgctttgt actctaggct gaagaagaaa cctaggaaaa
    10981 agtagattag cagatgacca tcattatgag caaacgcaaa cagcaaagag ctgatcactg
    11041 ctccgaccca aaaatttgaa tatcgactga tgatccccag tatactgcgg cggaatacga
    11101 actcttccat gatgggtcca gcaacagttg tcgccaaaat aaaagcgggt gcttccatga
    11161 tcatctggat gatattttgt gtattctcgg aaggggttgc tttgccaaac aatacagatt
    11221 ctaacatgac agcgatgctt tgcagaatca atgcgacaaa gattccaacc aatccataaa
    11281 taacaatctt tggagcagaa gcttttttta ccgctgattc gatcgctaag ggttcacgtt
    11341 gcttaaaata aaaaatagct aggacaacgg cgcctagtat atacatgact gcagttgctg
    11401 tgatcaattc agacgtagat tttacaagtc ctatcgaagc taggatcaag ggtgagataa
    11461 aaattagacc atagcaaaaa atgctgagga agctatattt ttttaaagac atgtaattcc
    11521 tccttttaag ggataacaag catgcaaggc aagcttatat cgattgattt caattatatc
    11581 ataatttcaa taagcagctg tcagaataaa atttcttttc tttgatttaa aagcttgcaa
    11641 aaaagaggga gaatgagtat tattaaaaat gtaggttagc actcgaaggt taagagtgct
    11701 aacagattaa aaaaagagtt atcggaggga tttatcgtgt taaaaccatt aggtgatcgc
    11761 gtcattatcg aagtcgcaaa agaagaagaa aaaactgtcg gaggaatcgt tttagcttct
    11821 gctgctaaag agaaacccca aactgggaca gttgttgcag tcggtgaagg ccgtttgtta
    11881 gaaaacggtg aaaaagttcc tgccgctgta aaagcaggcg accaagttat gttcgaaaaa
    11941 tacgccggta cagaagtaaa atacgaagga aaagaatatc tgatcgtcgc aggaaaagat
    12001 atcatggcga ttgttgagta aatttggaag ataaaaaaat aatcaaaaat aatcaatgag
    12061 gtgaatcaat catggcaaaa gaattgaaat ttgcagaaga cgcacgcgca gctatgttac
    12121 gcggggtaga taaattagca gatacagtga aagtaacatt aggacctaaa ggccgaaatg
    12181 tcgtactaga aaaatcttac ggctctccat tgatcacaaa tgatggtgtg acgatcgcaa
    12241 aagaaatcga attagaagat cattttgaaa acatgggtgc aaaacttgta tcagaagtag
    12301 catcaaaaac aaatgatatt gcaggagacg gtacaacgac agcaactgtt ctgacacaag
    12361 caatcgttcg cgaaggttta aaaaacgtaa ctgcaggtgc taatcctcta ggcattcgtc
    12421 gcgggatcga attggcaaca aaagcagcag tcgaagaatt gcataatatc tcaactgttg
    12481 ttgattcaaa agaagccatt gcacaagtag cagctgtttc ttcgggttct gataaagttg
    12541 gacatttgat tgctgatgca atggaaaaag ttggaaacga cggtgtcatc acaatcgaag
    12601 aatcaaaagg gatcgaaaca gaactagatg ttgtagaagg tatgcagttt gatcgtggtt
    12661 acttatcaca atatatggta acagataacg acaaaatgga agctgttcta gaaaacccat
    12721 atatcttgat cacagacaaa aaaatctcta acatccaaga tattctgcca ttgttagaac
    12781 aaatcttgca acaatcacgt ccattgttga ttattgctga cgatgtagat ggtgaagcat
    12841 tgccaactct tgtattgaac aaaatccgtg gaacattcaa cgtagtagct gtaaaagctc
    12901 ctggtttcgg cgatcgtcgt aaagcaatgc ttgaagatat cgctattttg acaggtggta
    12961 cagtaattac tgacgatcta ggtttagaat tgaaagatgt aacaatcgaa aacttaggaa
    13021 atgcttctaa agttgttgta gataaagaca atacaacgat cgtagaaggt tctggagaaa
    13081 aagaagcaat cgaagctcgt gtgcaattga tcaaaaacca aattgctgaa acaacttctg
    13141 attttgaccg cgaaaaatta caagaacgct tagctaaatt agcaggtggt gtagctgttg
    13201 ttaaagttgg tgcagctact gaaacagaat tgaaagaatt aaaattacgc attgaagatg
    13261 cattgaatgc tacacgtgcc gcagtagaag aaggaatggt ctcaggtggt ggtacagctc
    13321 tagtgaacgt tatcagcaaa gtttctgctg tagaagcaga aggagacgtg gcaacaggta
    13381 tcaagatcgt tgttcgtgct ttagaagaac caattcgtca aatcgctgaa aacgctggtt
    13441 atgaaggatc agtgattgtt gataaattga agaacgtgga actaggcact ggtttcaatg
    13501 ctgctactgg agaatgggta aacatggttg aagcaggtat cgtagaccca actaaagtaa
    13561 cacgttctgc cttgcaaaat gccgcttccg tttctgcctt gttattaacg acagaagcag
    13621 tcgttgcaga taaaccagaa ccagcagcgc cagcagctcc tgcaatggat ccatcaatga
    13681 tgggcggtat gatgtaagaa aaagcaactt gcttatctac aactgatcat tagtgaaaga
    13741 cagataaaaa gaggctggaa cagaagcgtt taactccaag aaataagaag aaattcacgg
    13801 aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc
    13861 cgctgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg tcccacgctc
    13921 ttttttgtgt gtaattataa tgaaaatagt tatttttaat agttataata ttttatctat
    13981 taaaagtaaa tatccattc
//
LOCUS       DACECZ010000070        13751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.070, whole genome shotgun sequence.
ACCESSION   DACECZ010000070 DACECZ010000000
VERSION     DACECZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.070"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            19..936
                     /locus_tag="IWO35_001829"
     CDS             19..936
                     /locus_tag="IWO35_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0316886.1"
                     /translation="MPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILT
                     FLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTD
                     YDFAHLLFSNGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVIS
                     TFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDD
                     LLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLY
                     GRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            939..3116
                     /locus_tag="IWO35_001830"
     CDS             939..3116
                     /locus_tag="IWO35_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0316887.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            3113..4114
                     /locus_tag="IWO35_001831"
     CDS             3113..4114
                     /locus_tag="IWO35_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0316888.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            4111..5043
                     /locus_tag="IWO35_001832"
     CDS             4111..5043
                     /locus_tag="IWO35_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0316889.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5288..5404
                     /locus_tag="IWO35_001833"
     CDS             5288..5404
                     /locus_tag="IWO35_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0316890.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5420..7339
                     /gene="tet(M)"
                     /locus_tag="IWO35_001834"
     CDS             5420..7339
                     /gene="tet(M)"
                     /locus_tag="IWO35_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0316891.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7458..7625
                     /locus_tag="IWO35_001835"
     CDS             7458..7625
                     /locus_tag="IWO35_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0316892.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(7685..8038)
                     /locus_tag="IWO35_001836"
     CDS             complement(7685..8038)
                     /locus_tag="IWO35_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0316893.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSIM
                     ESLASGINEARNIED"
     gene            8543..8965
                     /locus_tag="IWO35_001837"
     CDS             8543..8965
                     /locus_tag="IWO35_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0316894.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            8962..9192
                     /locus_tag="IWO35_001838"
     CDS             8962..9192
                     /locus_tag="IWO35_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0316895.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            9653..9856
                     /locus_tag="IWO35_001839"
     CDS             9653..9856
                     /locus_tag="IWO35_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAQ0316896.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            9938..11155
                     /locus_tag="IWO35_001840"
     CDS             9938..11155
                     /locus_tag="IWO35_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0316897.1"
                     /translation="MSEKRRDNRGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            11387..11713
                     /locus_tag="IWO35_001841"
     CDS             11387..11713
                     /locus_tag="IWO35_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0316898.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            11781..12494
                     /locus_tag="IWO35_001842"
     CDS             11781..12494
                     /locus_tag="IWO35_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0316899.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            12804..13655
                     /locus_tag="IWO35_001843"
     CDS             12804..13655
                     /locus_tag="IWO35_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0316900.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
BASE COUNT         4654 a         2365 c         2835 g         3897 t
ORIGIN      
        1 ggcagatgga aagaaacctt gccagagatt tcccatgaaa tcaatatcgt cactctgact
       61 tctgatgaga aaaacaaagg cttacttgac ccttatgtga ttatgaaaaa tcccaaagat
      121 tctgaatcac tggctattga tattctgaca ttccttacgg ggatttcctc tcgtgatggg
      181 gaacgcttcc caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtacgaggg
      241 ttgatgaaag tgattgagga attacgggtt gagaatacgc cactaagtac cagtatagcc
      301 gaccatatcg aaagttttac agactatgac tttgcacatt tattattcag taatggttat
      361 gtggagcagt ctatcagctt agaaaaacaa ctgaacatta tacaggttgc ggacttggta
      421 cttcccgaca aggaaacttc ctttgaggaa tataccacta tggagctttt atccgttgct
      481 atgctgattg tcattagtac ctttgcttta gactttatcc atacagaccg aagcattttc
      541 aagattgtag atttagacga agcatggagc tttttacagg tagcacaagg aaaaacacta
      601 tctatgaagc tggttcgggc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa
      661 aatacagacg acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgcattt
      721 cgttccactg accttaacga gattaaaaag accttagcct tttttggtgt agacccagag
      781 gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat
      841 ttatatggtc gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc
      901 tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atagtaaaca
      961 gaataaaatc aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca
     1021 cacttgtgat tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct
     1081 tggtagatga tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc
     1141 aactggattt ttatgtggat aatagctggg gctggcttcc gtggaactgg tcggacggga
     1201 ttggaaaaca ggtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtt
     1261 tgtatgtttc caatgcgaca ggttacttag tacaggaagc ctattcctta gacttcattt
     1321 ccgctacagc agattccatt ggtaagaata tgcagacctt agctggtgtg agtgcaaacg
     1381 gattttcaac agagggtttc tatgttggat tcctcttact cttgattttg gttcttgggg
     1441 tttatgttgc ctatacggga ctgataaaga gagaaaccac aaaggcaatt catgccatta
     1501 tgaattttgt gctggtgttt atcctatcgg cttcctttat tgcctacgct cccgactaca
     1561 ttaaaaaaat caatgacttt tcatcagaca tcagtaatgc cagtttatca cttggcacga
     1621 agattgtcat gccccattcc gatagtcaag gcaaggacag cgtggactta atcagagata
     1681 gcctgttttc catacaggtt cagcaaccgt ggctactgct tcaatacaac agttcagaca
     1741 ttgaaagtat cggtattgac cgtgtggaaa gcctgctctc caccagccca gattccaaca
     1801 atggcgaaga cagagaaaaa attgttgcgg aagaaattga agacagaagc aataccaatc
     1861 taaccattac aaagaccatt aaccgtttag gtacagtctt cttcctattt gtcttcaata
     1921 ttgggatttc catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt
     1981 ttatcatcta tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg
     2041 atggtatgtc aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa
     2101 tcacattgat tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg
     2161 gttatccgtt ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttca
     2221 agctgggcga tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa
     2281 gtcgtgtgat gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga
     2341 aacttggacg ttccatgact actctagggg ctgggtctgc cattgttaca ggtaaaaaag
     2401 gacagtcggg ttcggggagt tctgcaagga cacaagcaga tcactcccga ccagacggaa
     2461 aggaaaaatc aacacttgga aaacgtatcg gtcaaaccat cggtacagta gctgatacca
     2521 aagacagaat ggtagacact gctagtggtt tgaaagaaca ggttaaagat ttgccgacca
     2581 atgcaagata tgcagtatat caaggaaaat ccaaagtaaa agagaatgtc cgtgatttaa
     2641 ccagtagtat ttctcaaacc aaagcggaca gagccagtgg acgcaaggaa cagcaggaac
     2701 aaaggcgaaa aaccattgcg aagcgtcgct ctgaaatgga acaggtcaaa cagaaaaaac
     2761 agcctgcttc ttctgttcat gaaagaccga ctacaagaca agaacaatat catgatgaac
     2821 agacctcaaa acagtctaat attcagactt catataagga atctcaacaa gccaaacaag
     2881 agcgtccagc agttaagtcc gatttttcaa gtccaaaagt ggaacgccaa ggcaataccg
     2941 ttcaagaaaa aaccgttcaa aagccagcaa cttcaaccac tacagcagat agaacttcac
     3001 aacgtccaat cacaaaagaa cgtccgtcta ctgttcaaag agtaccacta caaaatacaa
     3061 gaagtagacc accaatcaaa accgccacca ttaagaaagt cggtaagaaa ccatgaagtt
     3121 gaaaacttta gtgattggtg gttctggatt attcttgatg gtcttctcac tgcttctgtt
     3181 tgttgccatt ttattttcag atgaacagga cagcggaatt tccaatattc attatggagg
     3241 tgtgaatgtt tccgcagaag tgctggctca taagcctatg gtagaaaaat atgccaaaga
     3301 atatggcgtt gaagaatatg tcaacatact tcttgcgatt atacaggtgg aatcgggcgg
     3361 tactgcggaa gatgttatgc agtcctcgga atccctcggt cttccaccta attcattgag
     3421 tacagaagaa tccattaagc aaggtgtgaa gtatttcagt gaattattag ccagtagcga
     3481 aaggctcagt gtagatttag aatcggttat ccagtcctac aattatggtg gtggtttctt
     3541 agggtatgtg gctaatcgtg gaaataaata tacctttgaa ctggctcaaa gtttctcaaa
     3601 agagtattca ggtggcgaaa aagtgtctta ccccaatccc atagccatac ctatcaatgg
     3661 gggctggcga tacaactatg gcaatatgtt ttatgtgcaa ctggtaacgc agtatcttgt
     3721 cacaacagag tttgatgatg atacggtaca agccatcatg gacgaagcac tgaaatatga
     3781 gggctggcga tacgtttacg gtggagcttc cccgactact tcttttgatt gtagcggact
     3841 gacacaatgg acgtatggaa aagctggaat taacttacca cgaaccgcac aacagcaata
     3901 tgatgtgacc cagcatatcc cactatcgga agcacaagct ggcgatttgg ttttctttca
     3961 ttctacctat aacgctggct cttatattac tcatgttggg atataccttg gcaataaccg
     4021 tatgtttcat gcaggcgacc caatcggtta tgccgactta acaagcccct actggcaaca
     4081 gcatttagtg ggagcaggac gaatcaaaca atgagaaagg aagatttaat gatgaaattt
     4141 agaaaaaatc agaataaaga aaaacagata ccaaaggaaa agaaacctcg tgtctataag
     4201 gtcaatcctc ataaaaaggt tgtgattgcc ttgtgggtac ttttagggct tagtttcagc
     4261 tttgcgatat tcaagcactt tacagctata gatactcata ctattcacga aacaactatc
     4321 atagaaaagg aatacgttga tactcatcat gtagaaaatt ttgtagagaa ctttgcgaaa
     4381 gtctactatt catgggagca atccgataag tccattgata atcgaatgga aagtctaaaa
     4441 ggctatctga cagatgaact tcaagctctc aatgttgata cagtacgcaa agatattcct
     4501 gtatcgtctt ctgtaagagg atttcagata tggacggtag agccaactgg cgacaatgag
     4561 tttaatgtaa cctacagtgt agaccagctc attacagagg gagaaaatac aaagaccgtc
     4621 cactctgctt atatagtgag tgtctatgta gatggttctg gaaatatggt actggttaag
     4681 aatccgacca ttaccaacat acctaagaaa tcaagttata aaccaaaagc cattgaaagt
     4741 gaggggacgg ttgattccat tacaaccaat gaaatcaatg agtttttaac gacgttcttc
     4801 aagctctatc ctacagcgac agccagtgaa ctttcctact atgtgaatga cgggatatta
     4861 aaaccaatcg gaaaagagta catctttcaa gaactggtaa atcctattca caatcgtaag
     4921 gataatcaag tcacggtatc gctgacagtg gagtatatcg accagcagac caaagcaacg
     4981 caggtatctc aatttgattt ggtacttgaa aagaacggga gtaattggaa gattatagaa
     5041 taacaaatat tggtacatta ttacagctat tttgtaatca cgtactctct ttgataaaaa
     5101 attggagatt cctttacaaa tatgctctta cgtgctatta tttaagtatc tatttaaaag
     5161 gagttaataa atatgcggca aggtattatt aaataaactg tcaatttgat agcgggaaca
     5221 aataattgga tgtccttttt taggagggct tagttttttg tacccagttt aagaatacct
     5281 ttatcatgtg attctaaagt atccggagaa tatctgtatg ctttgtatgc ctatggttat
     5341 gcataaaaat cccagtgata agagtattta tcactgggat ttttatgccc ttttgggctt
     5401 ttgaatggag gaaaatcaca tgaaaattat taatattgga gttttagctc atgttgatgc
     5461 aggaaaaact accttaacag aaagcttatt atataacagt ggagcgatta cagaattagg
     5521 aagcgtggac aaaggtacaa cgaggacgga taatacgctt ttagaacgtc agagaggaat
     5581 tacaattcag acaggaataa cctcttttca gtgggaaaat acgaaggtga acatcataga
     5641 cacgccagga catatggatt tcttagcaga agtatatcgt tcattatcag ttttagatgg
     5701 ggcaattcta ctgatttctg caaaagatgg cgtacaagca caaactcgta tattatttca
     5761 tgcacttagg aaaatgggga ttcccacaat cttttttatc aataagattg accaaaatgg
     5821 aattgattta tcaacggttt atcaggatat taaagagaaa ctttctgccg aaattgtaat
     5881 caaacagaag gtagaactgt atcctaatgt gtgtgtgacg aactttaccg aatctgaaca
     5941 atgggatacg gtaatagagg gaaacgatga ccttttagag aaatatatgt ccggtaaatc
     6001 attagaagca ttggaactcg aacaagagga aagcataaga tttcagaatt gttctctgtt
     6061 ccctctttat catggaagtg caaaaagtaa tatagggatt gataacctta tagaagttat
     6121 tactaataaa ttttattcat caacacatcg aggtccgtct gaactttgcg gaaatgtttt
     6181 caaaattgaa tatacaaaaa aaagacaacg tcttgcatat atacgccttt atagtggagt
     6241 actacattta cgagattcgg ttagagtatc agaaaaagaa aaaataaaag ttacagaaat
     6301 gtatacttca ataaatggtg aattatgtaa gattgataga gcttattctg gagaaattgt
     6361 tattttgcaa aatgagtttt tgaagttaaa tagtgttctt ggagatacaa aactattgcc
     6421 acagagaaaa aagattgaaa atccgcaccc tctactacaa acaactgttg aaccgagtaa
     6481 acctgaacag agagaaatgt tgcttgatgc ccttttggaa atctcagata gtgatccgct
     6541 tctacgatat tacgtggatt ctacgacaca tgaaattata ctttctttct tagggaaagt
     6601 acaaatggaa gtgattagtg cactgttgca agaaaagtat catgtggaga tagaactaaa
     6661 agagcctaca gtcatttata tggagagacc gttaaaaaat gcagaatata ccattcacat
     6721 cgaagtgccg ccaaatcctt tctgggcttc cattggttta tctgtatcac cgcttccgtt
     6781 gggaagtgga atgcagtatg agagctcggt ttctcttgga tacttaaatc aatcatttca
     6841 aaatgcagtt atggaaggga tacgctatgg ttgtgaacaa ggattgtatg gttggaatgt
     6901 gacggactgt aaaatctgtt ttaagtatgg cttatactat agccctgtta gtaccccagc
     6961 agattttcgg atgcttgctc ctattgtatt ggaacaagtc ttaaaaaaag ctggaacaga
     7021 attgttagag ccatatctta gttttaaaat ttatgcgcca caggaatatc tttcacgagc
     7081 atacaacgat gctcctaaat attgtgcgaa catcgtagac actcaattga aaaataatga
     7141 ggtcattctt agtggagaaa tccctgctcg gtgtattcaa gaatatcgta gtgatttaac
     7201 tttctttaca aatggacgta gtgtttgttt aacagagtta aaagggtacc atgttactac
     7261 cggtgaacct gtttgccagc cccgtcgtcc aaatagtcgg atagataaag tacgatatat
     7321 gttcaataaa ataacttagt gtattttatg ttgttatata aatatggttt cttgttaaat
     7381 aagatgaaat attttttaat aaagatttga attaaagtgt aaaggaggag atagttatta
     7441 taaactacaa gtggatattg tgtcctgtat gtggaaataa aacacgatta aagataaggg
     7501 aagatactga attaaaaaaa ttccccctct attgtccgaa atgcagacaa gaaaatttaa
     7561 ttgaaataaa gcagttcaaa gtaactgtga ttacagagcc agacgcaaag acgcagagcc
     7621 gataaaatga gattaataca atctcatttt atcggctctt tccgttatgt atggattctt
     7681 ttaattagtc ttcgatgttt cttgcttcgt tgataccgct ggctaaagat tccattatgg
     7741 atagttcttt gtctgtaaag ctatccatgt atttctctat ctgtaatcgt cgggtgcttt
     7801 ttaccaagtt attagcaggt aagaaaaatt catcaacgga aacatgaagt aacgatacaa
     7861 ggtcataaag aacttgtatg ctggggtgtt gccctttatt ttcaatatta gttaagtacc
     7921 gtgggtcaat ttcaatcaat gctcccactt gttcacgagt taaacctcgt ttcaatcgag
     7981 cttctttaat ggctaaacca aaggctctaa aatcatattt atcttctttt ttacgcatag
     8041 tagaccacct ctatacattt tattgttcct actgaattaa aaacaggtat agaaaaacgt
     8101 gttatatggt ttataggttt atatttaata aaaagcacta ctaaacgcca ataaaaaaaa
     8161 ccgttatatg gtagtgctat ttacgctgtt aaaatattgt atattacttc caaatggcgg
     8221 tttgttggag gtcaacgtcg ccatgaagta catcatatac aataaatttc cttacattgg
     8281 gttcttgtca aaaaaagtcg tctatctgca atagataagt acgtccacca atgtggtttt
     8341 ataaatcata tagatagaat aacagaagca tgtaaacaga gaaataaatc tgtttatatg
     8401 cttttttggc tattcagaac ttttttacaa agtttattta tcagtaatgc aacaaatccc
     8461 cctttcacat tgggactaag agtgaaagga gataaacgag caaggctcac ttcctttcct
     8521 agacagaaag ggggtgagaa acatgaaacc atcttctttt cagaccacaa tagaaaatca
     8581 gtttgactat atctgtaaac gtgctatgga agacgagcga aagaattata tgctttatct
     8641 ttcaaggatt gcaaagcgtg aggtgtcctt ttcggatgtt ggcgattatc ttgttagcca
     8701 gtttgcgaca acagataact attcaactga ctttcagatt tttacactca atgggttatc
     8761 agtaggcgtt gaaaatgatt tgttgagtga agcattacgt gagttgccag acaagaaacg
     8821 tgaaattcta ctgctgtttt actttatgga catgagcgat tcagaaattg cagacctgtt
     8881 gaaattgaac cgttctactg tctatcggca tagaaccagt ggactagcct taattaaaaa
     8941 gtttatggag gaatttgaag aatgaaaaca caatatccta tgattccctt tcctctcatt
     9001 gtaaaggcaa cagatggcga taccgaagcg attaaccaga ttctacatca ttacagaggg
     9061 tacataacga agcgttccct acgacttatg aaagatgaat atggcaatca aagtatggtc
     9121 gttgatgaag tcttacgtgg aagaatggaa accagactga ttacaaagat tttgtcattt
     9181 gaaattaagt aatatcctct ctcctttcgt ggaagcgtgc taaaccattc cacgcttccc
     9241 gaacagggag gtttgttatt ccaccaaagc atattgagct ttcaatgtgt tttgataggc
     9301 taacgagcca ttgttctttg aaaactgaat aaaagtaatc gaatacgttt cgataagaaa
     9361 agagccaacg gaactaaccg ccatgaccta tcttataaag atagcgagcg attcatgtta
     9421 gtgatccgag aagcaatctt tagcaggatt gcctgcaacg acattcttat cgtgataatg
     9481 atactcccat acagtcaata gtccgagcgt gataaaaccg tcgcaggcaa tgagtatggc
     9541 tacatgagaa ccatgcaggg gtggaactcc cgtgagcttt gctaaagctg ttcgattgct
     9601 ggtaaaacaa cttttatgaa atccaaataa gtgatttgga aaggaggatt ttatgaagca
     9661 gactgacatt cctatttggg aacgttatac cctaaccatt gaagaagcgt caaaatattt
     9721 tcgtattggc gaaaacaagc tacgacgctt ggcagaggaa aataaaaatg caaattggct
     9781 gattatgaat ggcaatcgta ttcagattaa acgaaaacaa tttgaaaaaa ttatagatac
     9841 attggacgca atctagcgtc gccaaagggt cttgtatatg ataaaatagt attaagtcgt
     9901 atcaaggctc tttccataaa ggaaaggagc aaatgccatg tcagaaaaaa gacgtgacaa
     9961 tagaggtcga atcttaaaga ctggagagag ccaacgaaaa gacggaagat acttatacaa
    10021 atatatagat tcatttggag aaccgcaatt tgtttactcg tggaaacttg tggctacaga
    10081 ccgagtacca gcaggaaagc gtgattgtat ctcacttaga gagaaaatcg cagagttaca
    10141 gaaagacatt catgatggta ttgatgttgt aggaaagaaa atgacactct gccagcttta
    10201 cgcaaaacag aacgctcaaa gaccaaaggt tagaaaaaac actgaaactg gacgcaaata
    10261 tcttatggat attttgaaga aagacaagtt aggtgtaaga agcattgaca gtattaagcc
    10321 atcagacgct aaagaatggg ctattagaat gagtgaaaat ggttatgctt atcaaaccat
    10381 caataactac aaacgttctt taaaggcttc attctatatt gctatacaag atgattgtgt
    10441 tcggaagaat ccatttgact ttcaactgaa agcagttctt gatgatgata ctgtccctaa
    10501 gaccgtacta acagaagaac aggaagaaaa actgttagcc tttgcaaaag ctgataaaac
    10561 ctacagcaaa aattatgatg aaattctgat actcttaaaa acaggtcttc gtatttcaga
    10621 gtttggtggt ttgacacttc cagatttaga ttttgagaat cgtcttgtca atatagacca
    10681 tcagctattg agagatactg aaattgggta ctacattgaa acaccaaaga ccaaaagtgg
    10741 cgaacgtcaa gttcctatgg ttgaagaagc ctatcaagca tttaagcgag tgttagcgaa
    10801 tcgaaagaat gataagcgtg ttgagattga tggatatagt gatttcctct ttcttaatag
    10861 aaagaactat ccaaaagtgg caagtgatta caacggcatg atgaaaggtc ttgttaagaa
    10921 atacaataag tataacgagg ataaattgcc acacatcact ccacatagtt tgcgacatac
    10981 attctgtacc aactatgcaa atgcaggaat gaatccaaag gcattacagt acattatggg
    11041 acatgctaat atagccatga cgctgaacta ttacgcacat gcaacattcg attctgcaat
    11101 ggcagaaatg aaacgcttga ataaagagaa gcaacaggag cgtcttgttg cttagtagta
    11161 caaatgaatt tactacttat ttaccacttc tgacagctaa gacatgagga aatatgcaaa
    11221 gaaacgtgaa gtatcttcct acagtaaaaa tactcgaaag cacatagaat aaggctttac
    11281 gagcatttaa gaaaatataa aaagataatt agaaatttat actttgtttt atgtaaaaaa
    11341 atggtatgat taatcaagta agaagtgatt ggaagaaggc aagcaaatga atagtatgga
    11401 aaaagtcaac cagtttagag atgaacgtaa ttggcgacaa tttcataacg aaaaagattt
    11461 ggctatctct atttctttgg aagcatctga attgttagaa ctttttcaat ggaaaacgcc
    11521 ggaagaagta aaagaaacac aattagaacg aatcaaagaa gaactggcag atgtattgat
    11581 ctattcatac atgatggcag acaaccttca cctggatcta gatgagatca ttgaagaaaa
    11641 attgatcaaa aataatttga aatatcctgt agaaaaaagc gcaggtgtac gaaaaaaata
    11701 cacagagttg tagaaatgtg tcattcggct gttggaaaga gcaaaagaaa aacagtaaaa
    11761 tgagtctgga gggagagaaa atgaaaaaaa aaggaaaagc aaaaagatgg ctagtcaata
    11821 tcctcttgtt cctcttgtta ttagtaggac tagctttgat tttcaatgaa cagatcaagg
    11881 attattttgt cagagagaca ggagataaat atgctatagc gaatgtaaca aaagaagatc
    11941 tgaaaaagaa taacgataaa gatgtcagtt ttgactttga tgcggtagaa ccaatgacga
    12001 cagaaggggt catgaggtcg caaatgagag gaacagatct accagtgatt gctagtatcg
    12061 cagtcccttc agtttcaatc aatttaccgg ttttcaaagg cttggataat acgtcactac
    12121 tttacggagc aggaacatta tctccagatc aagaaatggg aaaaggaaat tatgccttag
    12181 caagccatcg tgcaacgaac cctgaattgc tgtttacacc gctagaaaat ttagagatgg
    12241 gtgccaagat ctatttgaca gatttagaaa atgtgtatac atacaaaaca ttttttaaag
    12301 aaaaagttgc tcctacagat acccaattac tgaatgaagt cgagggaaaa gaaattgtca
    12361 cactaattac ttgtggcgac atggacgccg tcacgcgact agttgtacaa ggagaactgg
    12421 aaagtgttac atcgataaaa gatgcgacag atgatatgag atctgctttc aatctagaga
    12481 caaaaacatt ttaggatact gggacataag tcgttacaga ttaaaaacag ccaaaggata
    12541 tgaatcgata aggaaaaacc aatttgctga aaaaaagaga aacgcaaaga ggtgcatctc
    12601 tgatttggtt gttttctaga tagacttgtg ttccagtttt tttatttttt atatgaaaag
    12661 gcttgcataa ataaaaattc gatggtatga tgattttgaa taagaaatgg attgttactg
    12721 tacggcaggc aaaaccagaa ttcagctaag aaacagtagg cgaattctag gtttttttat
    12781 ttttcttgag agggcgaagc aaaatgaaaa ttgataaaat tttgaataac aatgtcgtca
    12841 tttcgaaaaa tggattcggg gaagaagtcg tttgtatggg aaaaggacta gcttttcaaa
    12901 aaaagattgg ggacgagatt tcgccagaag cggtccaaaa agaatttgtt ttaagagatt
    12961 cttttgctaa aaaccaattc cagcagctga tggctgatat tccggcagag gagatcgaat
    13021 gggtcaaaca agtagtcgaa ctggctgaag ataaactaaa agttgaattt tcgcctaata
    13081 tttatctgac attgaccgat catcttcatt atgcgatcac acgagcaaaa gaaaatatcc
    13141 aactgcctaa tccattatta tttgaaacaa aaaaatttta tccgaaagaa tatcaagcag
    13201 cgaaagaggc tcttttgttc gtgaaacaaa agacgggtgt ggaactttct gaggatgaag
    13261 ccggttttat tgcctttcat tttgtgaata gccaacaagg aaacgaagac atgcaagtga
    13321 cgatgaccgc taccacaatg gtaagagata tactgagtat catcagtaaa ttcttcggca
    13381 tcgtgttcga tgaagaatct ttgaattatc agcgtatcat tactcatctg cagttttttg
    13441 cccagcgtta tctcaaaggg gaaggatctg acgagcaaga tgaatttctc tacgcattag
    13501 ttcaaggaaa gtatccaaaa gcgtttcatt gtgtcgaacg gatcaatgag tatcttcgac
    13561 aaacgcaaaa acaggaaatg gggatcgcag agcagattta tttaacgatt catattcaaa
    13621 gagtagtaag tgagaaaaaa agcatccaat cataaggatt gttactgtat ggcaggcaaa
    13681 acctggattg aaaaaatagg ttcacgaatg tttgtgactg tttttttcaa tctgggtttt
    13741 tattttttaa a
//
LOCUS       DACECZ010000071        13747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.071, whole genome shotgun sequence.
ACCESSION   DACECZ010000071 DACECZ010000000
VERSION     DACECZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.071"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..863
                     /locus_tag="IWO35_001844"
     CDS             <1..863
                     /locus_tag="IWO35_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0316901.1"
                     /translation="MSQMMDFLSIFTDGMVAGSRILKIMDTEEYAPQQNPGADQMITS
                     GKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGSGKSSIINVLMRFYEFYEG
                     QILIDDHDIREYPMEELRKKMGMVLQDAFLFYGDIAGNIRMMNPAITDEQIRHAAEFV
                     QANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVTDPKILVLDEATANIDTET
                     EGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGHIVEHGNHETLLAQNGLYA
                     DMYKLQSSGI"
     gene            1189..2040
                     /locus_tag="IWO35_001845"
     CDS             1189..2040
                     /locus_tag="IWO35_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0316902.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEDLQVHLNDSVYIALTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSIHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFSCIEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            2137..4017
                     /locus_tag="IWO35_001846"
     CDS             2137..4017
                     /locus_tag="IWO35_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0316903.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVPFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTETAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYTMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(4057..4959)
                     /locus_tag="IWO35_001847"
     CDS             complement(4057..4959)
                     /locus_tag="IWO35_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0316904.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            5190..6593
                     /locus_tag="IWO35_001848"
     CDS             5190..6593
                     /locus_tag="IWO35_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0316905.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            6593..8026
                     /gene="ascB"
                     /locus_tag="IWO35_001849"
     CDS             6593..8026
                     /gene="ascB"
                     /locus_tag="IWO35_001849"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0316906.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQVKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            8171..9097
                     /gene="trxB"
                     /locus_tag="IWO35_001850"
     CDS             8171..9097
                     /gene="trxB"
                     /locus_tag="IWO35_001850"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ0316907.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            9311..11038
                     /locus_tag="IWO35_001851"
     CDS             9311..11038
                     /locus_tag="IWO35_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ0316908.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEEAKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            11110..11403
                     /locus_tag="IWO35_001852"
     CDS             11110..11403
                     /locus_tag="IWO35_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0316909.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(11495..12355)
                     /locus_tag="IWO35_001853"
     CDS             complement(11495..12355)
                     /locus_tag="IWO35_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ0316910.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLKQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            12447..12875
                     /locus_tag="IWO35_001854"
     CDS             12447..12875
                     /locus_tag="IWO35_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0316911.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13037..13213
                     /locus_tag="IWO35_001855"
     CDS             13037..13213
                     /locus_tag="IWO35_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ0316912.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            13240..13686
                     /locus_tag="IWO35_001856"
     CDS             13240..13686
                     /locus_tag="IWO35_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ0316913.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         4498 a         2349 c         3074 g         3826 t
ORIGIN      
        1 caatgtctca aatgatggac tttttgagca tcttcacaga tgggatggtt gctggtagtc
       61 gtatcttgaa gattatggat acagaagaat atgcaccaca acaaaaccct ggagcagatc
      121 aaatgattac ttcaggtaag attgagtttc gcaatgtaag cttttcttat gatggagagc
      181 acaatgtatt aaacaatatc tcttttgttg ctaatccggg agagaccgtt gcattagtgg
      241 gacacaccgg aagtgggaaa agctcgatca tcaatgtgtt gatgcggttt tacgaatttt
      301 atgaaggtca gatattgatt gatgatcatg acattcgaga atatccaatg gaagagttac
      361 gtaagaaaat ggggatggtc ttgcaagatg ctttcttgtt ttatggggat atcgcaggaa
      421 atattcggat gatgaatcca gctatcacag acgaacagat acgccatgcg gctgagtttg
      481 ttcaagcaaa caaattcatc gatgaattac caaaaggata tcactcaaaa gtgatcgaac
      541 atggtgccag ctattctagt ggacaacgac agctgatcac ttttgcgcga acaatcgtga
      601 ctgatcctaa aattctagta ttggatgaag caacagcaaa tatcgatact gaaacagaag
      661 gattgatcca agaaggattg gcgaaaatgc gacaaggacg gacaacgatt gcgattgccc
      721 atcggctgtc aacgattcga gatgcagaat tgattttagt tttagacaaa ggtcatatag
      781 tagaacacgg gaatcatgaa acacttctag cgcaaaatgg tttgtatgca gatatgtaca
      841 aactgcaaag cagtggtata taaaagatgt tgtgacagta gcattttgac ctaagcacta
      901 gagtgaaaat aaaacatagc ttcccatatt tttcatctgt gagagttagt gtcgtagtta
      961 ccagatttaa aaaagacgac gtcagctagt cagaagtgcc taatagcccc taagaaagcg
     1021 ttgacaaaat cgtttgcttt cgataataca aagaaaacga tacaacttgg atagtgattg
     1081 cctaatgcaa gctaaacctg atgagtctcc catggtttca tggacggctt tttgggtttt
     1141 tctttttgtt ttggatacag ccctatatat catggaaagg agcaaaacat gctgattgag
     1201 aaagtactga ataacaacgt cgtaataacc agagatgaaa gaaatcagga aatgatcgtg
     1261 atgggaaaag gtttggcatt caaaaagaaa gtgggtgacc cgatttttga ctcatgcatc
     1321 gacaaaaaat ttcagttgac aaatgacgga ctttcacaac agttccaaga attggcacaa
     1381 gcgattcctc ttgagtactt agaaatttct tgtgaaatca ttgaatatac aaaagaggat
     1441 ctacaagtcc atttgaatga ttctgtgtat attgcattga cggatcattt gtatacagct
     1501 atcgaacgtc aaaaaatggg gatctttgtg ccaaatgtat tgctgtatga tatcaaacaa
     1561 ctgtttccta aagagtatgc cattgggaaa aaaacagtga agaaaatctc ggataaatac
     1621 cacatcaagt tatcagaaga cgaagccgga tttatcgcat tgcatttagt caatgcacaa
     1681 ggcattcaaa gccagcaaga caatacctac ctgtttacca caacaataca ggatatttta
     1741 aatatcgtcc gctattacta taagattaca tttgatgaag attcaatcca tttttttcga
     1801 ttcgtcacac acctacgttt ttttctgaca cgcgtcctag atggagagaa atcagaagtg
     1861 ggagaagttt ctgaggaatt gttccaggtt attcaaaaaa aatataagga cacgtttagt
     1921 tgcattgaga aaatagcaga ctttttaacc ggtgctttcg actaccagat gtccacggat
     1981 gaaaaaatgt atttaacgat ccatactgcg cgtttagtgc atgagaatcg ttgtaaataa
     2041 ataaaaaagg aggacaaatt aacaagaacg tatgaaaata agggtagtga cattcagttg
     2101 gctaaacctt ccactaatta attggaggga atagaaatgg gaaaatacca ggaattagca
     2161 gaaaacatcg taaagaatat cggaggaaaa gaaaatatcc gtagcttgac acactgtgtg
     2221 acgcgacttc gtttcaaact aaaggaggaa aataaagcaa aggatgatgt tttgaaaaag
     2281 atggaaggga tagtcaccat catgaaaagc ggcgggcaat atcaagttgt aatcgggaat
     2341 catgtgccag atgtctatgc agaagtactg acagtagccg ggatttccga tgaagcagaa
     2401 gaagaggttt cttcaagtag taatctgttc aatcgattaa tcgacatatt aagtggctgt
     2461 ttccagcctt ttttaggtgc tttggctgca gcgggaatgg tcaaaggatt aaatgctttg
     2521 ttcttgtttc tcaaatttta tactgaagga tcgggtacgt acatcatgct gaatgggata
     2581 ggggatgcaa ttttttattt catgccagtg atcttagggt acacagcagc gaaaaaattt
     2641 catctgaatc cgatggtggg gctcgtgatt ggtgcagcac tttgttatcc gactgtacaa
     2701 ggagcagcac tacaaagtgg tttccctgat ggcagtcctg cgccttatag cttgctgggt
     2761 cttccggctt atgaaacatt tttgggtatc ccatgggtgg gcagcagtta taccagtagc
     2821 gtcgtacctg ttatcttcat tatcgcattt gccggacaag tccaaaaaat tgccaagaaa
     2881 tggattcctg cagtcgtcca gacctttttg gttccttttt ctgtgttatt aatcgcactt
     2941 cctgctggct ttttgattat cggtccgatt gtcagtatac tgacagatct attaagcacg
     3001 ggattccaag cattgatgga cttttctcca gctgtgtatg gtgcagttct agggtttctt
     3061 tggcaagtac tggtgatttt cggacttcat tggagtatta tccctcttgc gattatgcag
     3121 ctagcgcaaa atgggtggag tcagatgtta gtaggcgttt atggcgcgag ttttgcacag
     3181 acagcagtgg tattggcgat gtatttcaag ttcaaggatc aaaaaatgaa ggcgctttgc
     3241 atgccagctg tgatttcagg aatcttcggc gtaacagaaa cggctattta tggaatcacc
     3301 ttaccaaaga agaagccatt tatttactcg atgattggtg gtgctgtttc aggaatctat
     3361 ttgatggcat ctggaacaat cagctacaca atgggcgggt tagggatttt tggggtggta
     3421 aactacatca cggaaacaga cgccagtggt atgtttcaag cgtttatcgg cattgctatt
     3481 gcttcggtaa tcgggttttc gctgactttt ttcttttgga aggatgaagc aggtgaaaca
     3541 gatgatatac agaaaaaatc aggtaaagaa attgtaaaaa caccgataca aggacaaatc
     3601 gctccactaa atgcagcaaa ggatgcagct tttgcacaag gaacactagg tcgaggaatc
     3661 ttgatctacc ctgaaaaagg agaagtacgt gccccttttg acggaacagt catgaccctg
     3721 ttcccgacga aacatgcaat tggaatggtt tctgaaacag ggctggaatt gctgattcat
     3781 gtcggattgg atactgtgca gttggaaggg aaatattttg aaagcttagt gcagcaagga
     3841 gaaagagtga aacaaggcga tctgttgcta cgcttcgaca aaaagaaaat cgaaaaggaa
     3901 ggatacagtt tagaaacacc tgtgatcgtc accaattaca cggattattt ggatattttg
     3961 gaaaaccaag gggagacggt cgggccagac gatgcactta tcaccgcact gacctgaacg
     4021 gttaggtgaa gaatggacta ggcttaacta ttctttttat tccacttctt tattttcaaa
     4081 attgaaaggc tctttcatga tggctgagct gatagggcgt ttatcgaatt tttgaccgac
     4141 agaaattaga tggactaaat tctgttgata attttctgca aaaacacaac tatataacac
     4201 gtctagaaga taacaaattg atgtattgat cgtaaaatta ccaatttttg aatacagtcg
     4261 ctcgcgtgtc gtaagtcgca aagcggctgt actcattgca gatagcgttg aatcaccgat
     4321 actggtcaaa gctaaaattg gaacatcatt ttgtttcaat atttctgcgg tacgaaggat
     4381 cacattgttt tcacccgtat aagaaatcag aatcccgcac atatcactcg tgcaattgta
     4441 cgcttcatag aacgattcac caaaagtggt gacaatcgtg acatccattt tgatgcgctt
     4501 cattttcagc atgaaatctt gagaaatcaa agtattcgag ttactggcga aaatcacgat
     4561 tttttttgct tgaagcatta gttgtttggc tttttgcagt tggtcatgat gaatgagaga
     4621 aagtgtatca tgaatcgtca tctgttctaa agaagccatt ttatttgcga tcgtcataat
     4681 accgtctgtg gtggaaaaag gaagattcgc atctacgtct tgaaaattcc cttcaagata
     4741 ggctgtttca gctaaaaaag cattacgaaa actgacccag ccatcaaaac ccattttttt
     4801 tgctacacgg atgagtgtag acgggtggac atagcatcgc tcagcgatct cttggatggt
     4861 catttcgcca agtgctggag aatcgcttaa gagaaactgg atcacttcat tttctgaagg
     4921 agaaaaggag atttctttga ttttttcacg taataacatg tagttacctt cttcttattt
     4981 ttttacgtaa attataactt ttttgaaaat gttttgtaaa cggatttata ctatataact
     5041 taaaatagac tttttttgac aagatattta caaaaatcca ctagaaaaca gtaacttttg
     5101 taaatgttac tggaaagaaa cggatttcta ttgtttgttt tgtttacaaa aaagataatg
     5161 aaagtgtaaa aaagtaaagg agtgagaaca tggcaaaagg aatcaaaatc gctacgattg
     5221 gtggaggcag cagttacacc cctgagttga tggaaggatt tatcaaaaga tatgaggaat
     5281 taccgatacg agaaatctgg ctcgtagatg tagaagaagg aaaagaaaag cttgagatcg
     5341 ttagtcagat gtctcagcgt atgtgggatg cttctccgta tgaagtcaaa atctatgcaa
     5401 ctctggatcg ggaagaagca ttgaaagatg cggattttgt tacgactcag tttcgtgtcg
     5461 gtcaattaga tgcacgaatc aaagacgagc ggatccctag ttattatggg atgattgggc
     5521 aagaaacaaa tggtgctggc gggatattca aagcttttcg taccgttcca gtggtcttgg
     5581 caatcgttga agacatgaag cgtttatgtc caaatgcttg gctgatcaat ttcgcgaacc
     5641 caagtgggat ggtgacagaa gcagttatca gatatggcaa atgggataaa gtgattggat
     5701 tatgcaatgt gccagtaatg gcgatgatga cagaaccagc aatgcttggc aagaaaaaag
     5761 aagagctgat ctacaagttt gccggtctaa atcatttcca ttggcacaaa gtagcagata
     5821 ataaaggaaa tgaccgcacg gcagaattga ttgatcgttt atacgatgaa gacaatggat
     5881 tgccgaaaaa tatctttgat atcccgtttt tcaaagaaca attaaaacaa atgaatatga
     5941 tcccttgtgg ctaccaccgc tattactacc gtgaagaaga aatgctggaa catgctttag
     6001 aagaatatca aacgattggt acaagagcgc agcaagtgaa agaaacagaa gcagagctat
     6061 ttgaattata caaagatcca gaattagacc ataaacctga acaattgcaa aaacgaggtg
     6121 gcgcttatta ttcagacgct gcctgtgaaa cgattgcttc aatctatgga aataaagaaa
     6181 cacagatcgt ggtatctaca aagaatgatg gagctgttcc tgacctgccg gcagattgtg
     6241 tagtagaagt cactgcctat gttggcgcac aaggagcgag aaatgtagcg tttggttctc
     6301 ttcctgctgc tgaaagaggc tggttgcaag taatgaagaa tatggaatta ctgacgatcg
     6361 aagcagctgt gactggtgat tatgggacag ctttacaggc gtttacgatc aatccgctta
     6421 tacgatcagg aaagacagca aaacggatca tggatgagtt gtttattgca caccagcagt
     6481 atttaccgca attcaaggaa acaatcagcc gattaaaaga agaaggaatc gaagttcaag
     6541 atgaactggc aagagaatta tcaggtaata cacatataat ggaggaagta taatgggatt
     6601 cagaaaagat tttttatggg gcggtgctac agcagccaat caatgtgagg gcggttatga
     6661 tgaaggtgga cgcggactag caaacgtaga tgttgtgcca atcggaaaag accgttttcc
     6721 tgttatcacc ggaaagaaaa aaatgtttga atttgatgag gatcattttt atccagcaaa
     6781 aattgcaatc gatatgtatc atcgttataa agaagatatt gctttgtttg cagaaatggg
     6841 gttcaagact tatcgaatgt ccatcgcctg gagtcgtatt tttccaatgg gggatgaaaa
     6901 agagccgaat gaagctggct tgaaatttta tgaggatgtc tttaaagaat gccaaaagca
     6961 cggaatcgag ccacttgtga cgattacgca ttttgattgt ccaatgcatt tgatcaaaga
     7021 atacggtgcg tggcgttcca gagaactcgt tggtttttat gaaaatctgt gtcgagtcat
     7081 cttcaatcga tataaaggac ttgtcaaata ctggctgaca ttcaatgaga tcaatatgat
     7141 ccttcatgca ccattcatgg gagcaggact gtactttgaa gaaggagaaa acgaagaaca
     7201 agtcaaatat caagcggcac atcatgagtt ggttgcaagt gccatcgcaa cgaagattgc
     7261 tcatgaagta gatccggaga acaaagtagg ctgtatgctg gcagcaggag catattatcc
     7321 atacacttgc aatcctgaag atgtttgggc ttctagaaaa tcagatcgag aaaattattt
     7381 cttcattgat gttcagtcac gaggagaata cccagcttat gcattgaaag aaatggaacg
     7441 aaatggcatt cagatcgaaa tggaaccagg ggatgccgca ttgctgaaag aacatactgt
     7501 tgattttatc tctttttctt attattcttc ccgagtttcg actaccgaca acgaactctt
     7561 ggaacagact gcaggaaata tctttgcttc tgtcaaaaat ccatatctgc aagcaagtga
     7621 atgggggtgg caaattgatc cattaggctt gagaataacg atgaatgata tttatgatcg
     7681 ttatcaaaaa ccattattta ttgtagaaaa cggcttaggt gcagtcgata cgccagatga
     7741 gaatggttat gtggaagatg attaccgaat cgattacctg gcagcgcata tccaggcgat
     7801 gaaggatgca gtagaactag acggagtcga tctattaggc tatacgactt ggggatgtat
     7861 tgatttagta tcagctggta ccggtgagat gaagaaaaga tacggattca tttatgtcga
     7921 tcgtgataac caaggaaatg gcacattagc ccgttcaaag aaaaaatcat tcaactggta
     7981 taaaaaagta attgattcaa atggagaaaa tctttcgaac gattgatcaa tgatttataa
     8041 aagtctattg tttttcagcg gatttttcga gtataataaa gctattcgag aaacgcaagc
     8101 agaacaatag actttttttc gcattggctg taaagtatgc ttacttgatt aagtaaagga
     8161 gaatctacat atgtatgatg taatcgttat tggcgcaggt cctgctggga tgactgctgc
     8221 tttatatgct tcacgttcaa acttatccgt attgatgatc gaacaaggtg cccctggcgg
     8281 ccaaatgaac aatacggcag aaatcgaaaa ttatcctgga ttcgattcga tcatgggccc
     8341 cgatctagct tataagatgt atgaaagtgt ctcaagattt gggacagaaa atgcttatgg
     8401 aatcgttcaa gatatcaagg atcacggcag ccataaagaa gtcatctgtg aagataaaag
     8461 ttacgaagga aaaacagtca ttatcgcaac aggctgtatc caccgtaaat taggcgtacc
     8521 tggcgaggaa gaatttgctg gacgaggcgt ttcttactgt gcagtctgtg atggtgcgtt
     8581 tttccgtaac aaacgtctag tagtcatcgg gggtggggac tcagcagtag aagaagccat
     8641 ctatttgaca caatttgctt ctgaagtagt gatcgtccac cgtagagatg aattacgtgc
     8701 acaaaagatc atccaagacc gtgcatttgc aaatgaaaaa atttccttta tgtgggatac
     8761 agtcgttgac gaaatcgttg gaaacgaaat ggtagtgaca ggtgtgaaag cacgtaatgt
     8821 caaaaccggc aatgaatttg atttagcagc agacggagta ttcatctatg ttggactgga
     8881 tccattaact gcacctttta gagaagcagg tttgacgaac gaagctggct ggattccaac
     8941 agacaacgaa atgaaaacga atatgccagg cgtttttgcc atcggagatg ttcgtgaaaa
     9001 agatttgcga caaatcacta cagctgttgg tgacggcggt attgctggac aacaagtgta
     9061 taaatacttg gaatctttag aagaaacaac tgcataagtt taaaaagagt agaagaacgg
     9121 gttcaattgt tcttttactc ttttttttcg cgtgaaattt actgaatctg atttatgcga
     9181 acacgtccaa caccttttga taaaagaatc ctttcttgat ttaagcgttt tttggtcctc
     9241 atactttcat atctaataag tgaatgctat acttattaag aaccatattt cagaaagagg
     9301 tgttattttc atgtcttggg aacaagtgta tgaacaatgg gtacatgaag aaaatttaga
     9361 agaaaactta aaaaaacaat taaatgatct gggtgaagat cctgaaaaac ttgaagatgc
     9421 tttttatgca ccgttggaat tcggtactgc aggcatgcgg ggaatcttag gacctgggat
     9481 caatcgaatg aacatctata cggtaagaca agcaacagaa ggactagcac gtttcatgga
     9541 tacacaaggt gaagaagcaa aacgccgagg tgtcgctatt gcttatgact cacgccatca
     9601 gtcaccagaa tttgcaatgg aagcagccaa aacactggca catcacaata tcccgtctta
     9661 tgtattcgaa agtctgcgtc caacaccaga gctttctttt gcagttcgtc atttgaagac
     9721 gtttgcggga atcatggtaa ctgcatctca caacccagcg gcttacaatg gatataaagt
     9781 ctacggagaa gatggcggac aaatgcctcc tgcagacgca gatgcattga cgaaatatgt
     9841 ccgtgaagtc agcaatccgc taaaagtcga agtattatct gacgaagaag ctaaacatag
     9901 cgggttgatc acgatcatcg gagaagaagt ggacgctgct tatctagaag aaatcaaagc
     9961 agtgacgatt gatcgtgaat tggtagaaac aatggggaaa gatttgaaac ttgtgtacac
    10021 gccacttcat ggaacaggga aaatgttggg cgaacgtgcg ttgaaacaag ctggattcga
    10081 acaattcgta ctagttcccg aacaagctgt ggcagatcca gatttcacta cggtcaaatc
    10141 gccaaatcca gaagaacatt cagcttttga atatgctata cgacttggag aaaaagaagg
    10201 agctgacttg ctgattgcaa cagatcctga tgcagaccgt ttaggcgcag cggttcgttt
    10261 gccagatggt tcttatgaag tattgactgg gaaccaaatc ggggcattgt ttgctcaata
    10321 tattttggaa gcacatcaat caagaggcac tttaccagaa aacgcagcgg tattgaaatc
    10381 aatcgtttcc agcgaattgc ctacagcaat tgcggaacac tacggtgcta ccatgttcaa
    10441 tgtgttgaca gggtttaaat tcattgctga aaaaatccaa caattcgaag aagaccattc
    10501 acatactttt atgtttggat tcgaagaaag ttatggttat ttagttaaac cttttgttcg
    10561 cgataaagac gcgatccaag cattgctttt attagcagaa gtagcagctt actataaaaa
    10621 acaaggcaaa acgttgtatg acggcttgca aacgattttt gaaacctatg gctattttgc
    10681 agaaaaaacg atttctgtga caatgagcgg acaagaaggt tctgcgaaaa tctctgcttt
    10741 gatggaaaaa ttccgtaaag aagcaccgac agaatttgcc ggagcaaaag ttattcaaac
    10801 agaagacttt aaagaattga cgagaacaga ccgtgaagga aacgtagaaa aactaacaac
    10861 accgccatcc gacgtattga aatatacatt ggaagaaaat ggctggattg ctatccgccc
    10921 ttcaggaact gagccgaaaa tcaagtttta tattggtgta aaaggaagct ctacagaaga
    10981 cgcagccgaa aaagtagcct cttatgaagc gattatcaaa aagatgacag aagaataaaa
    11041 aacttgagac tgatggaaaa agtggtaaac tttagaagtc agtctttttt caagttggag
    11101 gtgaatggat tgccagacac gcaaaaatca aaagtagaga aagtcagtca attattcaaa
    11161 atgttgagtg atcccactcg tcttaaaatt ctgctatatc tgaaagacgg ggaacaaaat
    11221 gtgacagcaa tcacccaagc agtggaaatg gagcagtctg ctgtatcgca tcaactacgt
    11281 ttattaagag aaaatcatgt cgtcaagtct catcgcgagg gaaaagcgat tttgtatagt
    11341 cttgatgatt ctcatgtact ggatattttg aaccagacac tcaaacatgt ggaacatcaa
    11401 taacgatagg tgcaaataga taaacactgg agtgtgacaa cgaaatttct tcgttgtcac
    11461 actccagtgt tttttaagaa aaagacaaaa atgattaatc gcttttttct gttgtttcag
    11521 ttccataata actatgatct tctaggtcta aagcattcaa aataagcgaa gccgtttctt
    11581 caatagataa agatgccaca ttgatcacta cacatcctag tttttcataa agatcattcg
    11641 caaaatcgag ttcagctcta attttttcga tatcggaata agcagtatca gggtttaatc
    11701 cgtaagagcg catccgttct ttacggatat tatttaaaat atctgggtta ttcgttagtc
    11761 ccacgatttt tttaggatcg atctcccaaa gctgtttggg aatatgtgct tgtgggatca
    11821 aaggtaaatt ggcaactttt aaatttttgt tagctaaaaa caagctcaaa ggcgttttgg
    11881 aagttcggga tacgcctagc aaaacgacat ctgcttctaa aaaaccacga ggatctttcc
    11941 catcatcgta ttttacagca aattccattg ctttgatccg tttgaaatag ttctcattca
    12001 ataaatggag ggcaccaggt tgccgaattg gcttgatacc ggttaaacgt tcaatttctg
    12061 atatcggtgg cgtcatcaca tcaaaactaa ataattgatg ttcttcacaa aaagaacgtg
    12121 ttatctcgat cagttcttga ttgattaatg tatgcaggac aagtgctttt tcttctaatg
    12181 catcttgcaa tgcctgcttt aatgtttttt catctgtcac gaacgttctg cggaataggt
    12241 tgaactcaac ggtaggatac tgagccatag tagcttgcgc tagttttgtc gcagtttctc
    12301 cagcagagtc ggagatgaca aaaaaagtaa cgatttctgt agaatcggaa gacatggata
    12361 aaacctcact tgcatttttt ttatgtttat tataccatac tctagaatgc gtacttcgta
    12421 atcattttga tttggaggaa taagtcatga aaaaacagac attgatcgaa caatcattag
    12481 aaacattgaa aaataacggt tttaaatata ctaaaaaaag agaagcattg ttaacttatt
    12541 tagtgaagcg taaccgctac gtatctgcta aagaagtttt tgactatatg aatcaggaat
    12601 tcaaaggggt aagttatgat acgatttatc ggaatctcca tgattttgca gagatcgatt
    12661 tactggaaga aaccgattta aatggagaaa tgaagtttcg ttttcattgc tgtcaggggg
    12721 aaatcggcca tcaccatcat tttatctgta cagtgtgcgg aacaacgaaa gaaatccata
    12781 tgtgtccgat gaattatttc gaagaacagc taggtggatg cacgatcgaa gggcatagat
    12841 tcgagatttt cggaagatgt gaaaattgtc aataagggtt taaataaaat aacatttgat
    12901 aattttaaaa aaaagcaaga tttccttgac ggtcaaaaca gcattagata taatatttac
    12961 aggacagtct tgttttttga gatatatttc aataacaatt acggttactt tttaagctcg
    13021 gagggaggga attaacatgt caaaaacagt cgttcgcaaa aacgaatcac ttgacgatgc
    13081 tcttcgtcgc ttcaaacgtt ccgtgtcaaa agcgggtact ttacaagaat ctcgtaaacg
    13141 tgaattctac gaaaaaccaa gtgtaagacg caagaaaaaa tctgaagcag ctagaaaacg
    13201 caaaaaattc tagttctgtg atgaattgga gttggtagtg tgtcactact aagtagatta
    13261 aacgatgaca tgaaagcagc gatgaaagcg aaagataagg aatctttaca agtcatccga
    13321 atgatcaagt catctataca aaacgagcag attaaagaag gtcacgactt gacagaagaa
    13381 gaagaactaa cggtgttgtc tcgcgaaatg aaacagcgac gtgattcttt gcatgagttt
    13441 gaagaagcag gacgtgacga tttagctgaa aaagtaaaaa gtgaaattgt aatagtcgaa
    13501 aaatatatgc cggaacaact ttccgatgaa gaaattcgac aacttgttca agaagcaatc
    13561 actcaaactg gtgcttcatc aatgaaagaa tttggcaaag ttatgggtgc aattatgcct
    13621 aaagtcaaag gaaaagcaga tggaaaccaa gtgaatgcga ttgtgaaaga actattacaa
    13681 tcttaatttt atcatttaaa ctgcggacag tgattgtccg cagttttttg tgttcgtaaa
    13741 aaaagaa
//
LOCUS       DACECZ010000072        13673 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.072, whole genome shotgun sequence.
ACCESSION   DACECZ010000072 DACECZ010000000
VERSION     DACECZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.072"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            43..285
                     /gene="yaaA"
                     /locus_tag="IWO35_001857"
     CDS             43..285
                     /gene="yaaA"
                     /locus_tag="IWO35_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ0316914.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            272..1396
                     /gene="recF"
                     /locus_tag="IWO35_001858"
     CDS             272..1396
                     /gene="recF"
                     /locus_tag="IWO35_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ0316915.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1393..3339
                     /gene="gyrB"
                     /locus_tag="IWO35_001859"
     CDS             1393..3339
                     /gene="gyrB"
                     /locus_tag="IWO35_001859"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ0316916.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3359..5830
                     /gene="gyrA"
                     /locus_tag="IWO35_001860"
     CDS             3359..5830
                     /gene="gyrA"
                     /locus_tag="IWO35_001860"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ0316917.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6066..6365
                     /locus_tag="IWO35_001861"
     CDS             6066..6365
                     /locus_tag="IWO35_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ0316918.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6415..6927
                     /gene="ssb"
                     /locus_tag="IWO35_001862"
     CDS             6415..6927
                     /gene="ssb"
                     /locus_tag="IWO35_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0316919.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6953..7189
                     /gene="rpsR"
                     /locus_tag="IWO35_001863"
     CDS             6953..7189
                     /gene="rpsR"
                     /locus_tag="IWO35_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ0316920.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7332..9305
                     /locus_tag="IWO35_001864"
     CDS             7332..9305
                     /locus_tag="IWO35_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ0316921.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9311..9763
                     /gene="rplI"
                     /locus_tag="IWO35_001865"
     CDS             9311..9763
                     /gene="rplI"
                     /locus_tag="IWO35_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ0316922.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10037..11404
                     /gene="dnaB"
                     /locus_tag="IWO35_001866"
     CDS             10037..11404
                     /gene="dnaB"
                     /locus_tag="IWO35_001866"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ0316923.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12184..13476
                     /locus_tag="IWO35_001867"
     CDS             12184..13476
                     /locus_tag="IWO35_001867"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ0316924.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4555 a         2478 c         3010 g         3630 t
ORIGIN      
        1 ataatagagt atacttttaa taatgaaaat gagggatggg cattgaaaca acaagtcatt
       61 ctaaatacgg aattcatgac gttgggccaa atgttgaaag aagtcacagt tatcggtagt
      121 ggcggacaag caaaatggta tttggcagaa aacactgtgt tagtcgatgg cgaaccagaa
      181 aatcgtcgtg gacgcaaact ttatcccggc atgatggttg aagttccaga agtaggaact
      241 ttttttatgg taaaaaaagg aaacgacgat aatgaggctg aataagctgt atttaaaaaa
      301 ttatcgaaat tacgaagagc tgaacatcga attttccaag aacctcgtca tctttttagg
      361 agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc tacgtattag ctatgacgcg
      421 aagtcatcgg acaagtagtg agcaggaatt gattggctgg actgacgatc aggcgatgat
      481 tcaaggagag atcacaaaag gttcatccac tcttccatta gaaatcttat tatctaaaaa
      541 aggccgaaaa accaagatca accatatcga acagaaaaag ttaagtactt acgtgggaca
      601 aatgaatgtc attttatttg caccagaaga tctttcttta gtaaaaggca gcccgcagat
      661 caggcggaag tttttagata tggaattagg ccagatcaat ccaatctatc tttatgatct
      721 tgtccaatac caagcaattt tgaaacaaag aaaccagtac ttgaagcaat tgaatgaaaa
      781 aaaacaaact gatttgcttt atttggatat tttgtccgaa caactagcag cgtttggcag
      841 caaagtattg aaagcaagag cacagttcgt caagcgattg gagttttggg ctaattcttt
      901 gcatcagcaa atcacccatc aaaaagagca gctggaaatc gagtatctca ccgcagtcga
      961 ttctttagaa acacatacgc aagagcaaat ccaagaacaa tttctcgcac ttttgaacca
     1021 aaataaaaag aaagaccttt ttcggggaac aacgacagtt ggtcctcatc gtgacgatct
     1081 ttcctttttt attaatcaga aaaatgtgca gacttatgga tcgcaaggac agcaaagaac
     1141 aactgcacta agtgtcaaat tagcagaaat cgatttgatt aaagaagaga caggggaata
     1201 tccaatcctt ttgttagatg atgtgatgag cgagctggat gataatcgtc aactccatct
     1261 gctagaaaca atcgaaggaa aagttcaaac gttcttaaca acaacgacac ttgaccatgt
     1321 aaaaaataaa atgactgttg aacctgagat tttttatgtt caacaaggga aactaaaagg
     1381 agaagaaact gaatgacaga agaaagaagt ttagtagaac gcgctaaaga gtatgatgcc
     1441 agtcagattc aagtacttga aggacttgaa gccgttcgta aacgacctgg catgtatatc
     1501 ggttcgacta gttctgaggg tctgcatcac cttgtttggg aaattgtcga taattcgatc
     1561 gatgaagtat tagcaggttt tgcaacgaaa atccatgtga ttattgaaaa agacaacagt
     1621 atcaccgtta ttgacgacgg tcgggggata cctgtagata tccaggcaaa aactggacgt
     1681 cctgcagttg aaacggtctt tactgtttta catgctggag gaaaattcgg cggtggtggg
     1741 tacaaagttt ctggtggact tcacggagta ggatcttctg ttgtaaacgc gctttctaca
     1801 cagcttgatg tcaaagtata caaagaagga aatgtgtact atcaagaata tcgtcgtgga
     1861 gctgtagtgg acgacttaaa gattatcgaa caaacggatc gccatggaac aacggttcac
     1921 tttacgccag accctgaaat ttttacagag acaactgagt ttgatttttc aaaattggct
     1981 acccggatcc gtgaacttgc tttcttgaac cgcgggatgc gtatatcgat tgaagacaaa
     2041 cgagaagaag aaccagtcat caatgaatac cattatgacg gcgggatcaa aagttatgtc
     2101 gaatacttga atgcaaacaa aaccgtgatt tttccagaac ctgtatattt ggaaggtgaa
     2161 cagcaagata tcgcagttga agtctctatg caatatacag acggctacca ttccaatatc
     2221 atgagttttg ccaacaatat ccatacgtat gaaggtggaa cacatgaatc aggattcaag
     2281 actgctttaa ctcgtgtgat aaatgattat gcccgaaaac aaaagctgat gaaagaaaat
     2341 gacgacaact tgaccggtga agacgtgcga gaaggattga cagctgttat ttctatcaag
     2401 catcctgacc ctcaattcga aggacaaaca aaaacaaaat tgggcaattc cgaagtacgg
     2461 acagtcactg atcggttatt tagcgaacat ttcatgaaat tcttgctgga aaacccaagt
     2521 gtgggccggc aaattgttga aaaaggatta ctagcttcca gagctcgtct agcagcgaaa
     2581 cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga ttagtaatct gccaggaaaa
     2641 ttagcggact gctctagcaa cgatcctgaa aaatgcgaat tgttcatcgt cgaaggagat
     2701 tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt ttcaagccat cttgcctatt
     2761 cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca agatcttggc aaatgaagag
     2821 atccggtctt tatttactgc tatgggtaca gggtttggag aagattttga tgtatcgaaa
     2881 gctcgctacc acaaattagt tatcatgact gatgccgatg tcgacggcgc gcatatccgt
     2941 acattgctgc tgactctttt ttatcgttac atgcgtccta ttgttgaagc tggatacgtc
     3001 tatattgctc agccaccttt atacggggta aaacaaggga agaatattac gtatgtacaa
     3061 ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg cttcacttcc tgcctcacca
     3121 aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg atgatcatca actatgggaa
     3181 acaactatgg acccaagcaa tcggatgatg gcccgcgtca gtgtggacga tgctattgca
     3241 gcagatcaga tatttgaaat gttgatgggg gatcgagtag aaccccgtcg tgcattcatc
     3301 gaggaaaatg cacactatgt gaagaactta gatatttaat catgaaggag gaaaaagaat
     3361 gagtgaagaa atcagagaaa acatccatga tgtcaatcta accagcgaaa tgaaagactc
     3421 cttcatcgat tatgcgatga gtgttattgt cgcacgtgca cttcctgatg tcagagatgg
     3481 tctgaaaccc gttcatcgcc gtattttata cggcatgaac gaattaggtg taacaccgga
     3541 taaagcccat aaaaaatctg ccagaatcgt cggagatgtc atgggtaaat accatcctca
     3601 tggagacatt gcgatctatg agtcaatggt acggatggca cagccattca gctaccgtta
     3661 tatgctagtg gatggtcatg gaaactttgg ttctgttgac ggtgacggtg ccgccgctat
     3721 gcgttatacc gaagcacgga tgagcaaaat cgctacagaa atgctgcgtg atatcaataa
     3781 gaacactgtt gatttccaaa gcaactatga tgatacagaa aaagaaccag ttgtactacc
     3841 tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg atcgccgtag gtatggccac
     3901 gaatatccca cctcataact tgtctgaggt agtagcggct atcgatttgc tgatggataa
     3961 tcctgatgtg acgaccaatg aattgatgga agtattacct ggaccagatt ttccaacagg
     4021 tggtttggtt atggggaaat caggaatccg aagagcgtat gaaacaggta agggttctat
     4081 taccgtacgt gcaaaagttg agattactga gatgccaaat ggtaaagaac ggatcatcgt
     4141 gacagaattg ccttatatgg tcaataaagc aaaattgatc gaacggattt ctgaactgca
     4201 cagagacaag cgtatcgaag ggatcacaga tttgcgggac gaatcttccc gtgaaggtat
     4261 gcggatcgtc attgacatcc gtcgtgacgc cagcgcatcc gttatcttga ataacttata
     4321 taaattgaca tccttgcaaa attcatttgg attcaatatg ttggcgatcg aaaaaggtgt
     4381 gccgaaagtt ttgagcttga aacaaatcct tgaaaactat atcgaacatc aacgcgaagt
     4441 catcacacga cggacggtct ttgaaaaaga aaaagcagaa gcaagagcac acatcttaga
     4501 aggtttgcgt attgctttgg atcatatcga tgaaatcatt gcgattatcc gcgggtctaa
     4561 atcagatgac gaggcaaaag caacaatgat cgaacggttc gatttatccg atcgccagtc
     4621 tcaagctatt ttagatatgc gtttacgccg attgaccggt ttggaacggg aaaaaatcga
     4681 aaatgaatac caagaactct taaaactgat tgaagattta actgacattc tggcaagacc
     4741 agaacgtgtt acagaaatca tcaagaatga attagcagac ttgaatcaac gatttggtga
     4801 taaacgacgc acagaattac tagtcggtga ggtcttaagt ctggaagatg aagatttaat
     4861 cgaagaagaa gaaatcgtta ttacattgac aaacaatggt tacatcaaac gtttggcaaa
     4921 taatgaattc agagctcaac gaaggggtgg acgtggtgtc caaggaatgg gtatccacaa
     4981 tgacgacttc gtcaaaaatc tggtttcttg ttctacccat gatacattgt tattctttac
     5041 gaataacgga aaagtgtatc gggcaaaagg atacgaaatt ccagaatacg ggcgtacagc
     5101 caaaggaatc ccaatcatca atttactagg tatcgattcg agtgaaagca tccaggcaat
     5161 cattgcggtg acgggagaag cagaagaagg ccactatctc ttctttacga cgcgtcaagg
     5221 tacagtaaaa cgtacttctg tcactgcttt tgctaacatc cgaagtaatg gtctgatcgc
     5281 aattggtttg aaagaagacg atgagctcgt caatgtgcta ctgacagatg gtcaatctaa
     5341 tatcattatt ggaacacata acggctactc tgtcactttc aaagaagaag cagtccgcga
     5401 tatgggacgt acagcttccg gggtcagagg gatacgcctg cgtgaggaag attatgtcgt
     5461 tggggcagcc ttaatgaagg aaaatcaaga agtattagtg atcacagaaa aaggttacgg
     5521 aaaacggaca aaagtatcgg aatatccagt gaaaggccgc ggaggtaaag ggatcaaaac
     5581 ggcgaacatc actgaaaaaa atggtccact agctggattg accactgttt ctggagatga
     5641 agacatcatg ctgattacag ataaaggtgt cattatccgt ttcaacgtat cgactgtttc
     5701 tcaaactgga cgttcaacat taggcgtacg tttgatgaag atggaagctg atacgaaagt
     5761 tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa acagttgaag aacaaggatc
     5821 agaagaataa ccatcaagac taagtgtgta agctagcaag aataaccgat taaaaaatct
     5881 ggttattcct gctggctttt ttgattagtt gaatttctct taaaatattc gaaactgttc
     5941 attctgtatt gcttttgacc aaaatagagt gtataattag agcttgtgag tagtctaatt
     6001 aattagataa ctctccttgc tcttaggaac ctaagagccg aaagtccaca gggaggtgaa
     6061 tatcaatgga aaacacgaaa tatgaaatca tgtacattat tcgtccaaac attgatgaag
     6121 aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt gaaagataac ggagcagaag
     6181 ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta tgaaatgaac ggttaccgtg
     6241 aaggtatcta tcacatcgtt aaagtatctt ctccatcatc agcaaatgct gtcaatgaat
     6301 ttgaccgtct tgctaaaatc aacgacgata tcattcgtca catgattgtt aaagaagaag
     6361 cttaattgtt gtttcatgtg aaacaataga aaacgatgag aggagatgac tattttgatt
     6421 aataatgttg tattagttgg acgtctgacg aaagatccag atttgcgtta cacagcaagt
     6481 ggtacggcag tcgcaacatt tactttggct gtgaatcgta attttacaaa ccaaagcggc
     6541 aatcgcgagg ctgattttat caactgtgtg atttggcgca aatcagcaga aactttagca
     6601 aactatgctc gtaaaggaac acttttaggt gttactggac gtatccaaac tcgttcttat
     6661 gataatcagc aaggacaacg tgtatacgtg acagaagtag tagctgacaa cttccaattg
     6721 ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg gcggattcca aagttctggt
     6781 caatcagctg gcggatttgg cggaaacaat aactctaatc aaacatctca atcatccaac
     6841 ggtatgcctg atttcgaccg tgatacttct gacccattcg gctcaagttc aacgatcgac
     6901 atttcagacg atgatttacc attctaaacg aatttgatag gagggaaata gaatggcaca
     6961 acaaagaaga ggcggacgta aacgtcgtaa agtagactac attgctgcta accatattga
     7021 atatatcgat tataaagata tcgaattact aaaaagattt atttcagaac gcggtaaaat
     7081 cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa cgtaaaatta ctgttgcaat
     7141 caaacgcgct cgtatcatgg gcttgctacc tttcgttagc gaagactaat aaatcctagg
     7201 aattgaatga ctgatccttt tgatcagcta ttcaattcct ttttttctgt aaaaataacc
     7261 tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa aatcaaatta gtcatatatg
     7321 ggaggaaaat tatggaaaat aaaaacaatc atttatcccc gaaacctctc ttccttatcc
     7381 tgtttatatt aatcgaagta ttggtagtct taattttacc atttcgtttt gttagcgctt
     7441 cggtcattct attgatgaat ttatggggtg ttcgctattg gttgaaaatg aaacgttggc
     7501 ttattttaaa taaacgtgaa aaaatccagc aggcttcgac tgtagctgaa accaatctag
     7561 cttatgtctc agaagtcatg cctgtaggag tgattgctta tcagccatca agccaaaaaa
     7621 tcgaatggct caatccatta gcattgaaaa tcaaagatca aagtggatta acagaggaag
     7681 aattgctaga tacttttttt actgcaaaag aaaaaaagaa aaaccaagtc tatctagcag
     7741 ataaaacatt tcagtttgat atggatacag taaagggtgt tgtttatttt aatgatatta
     7801 cccaaaacca gttgttaaaa ctccatcaga cgaacagtca gccggtgatt ggtattttat
     7861 ctattgataa ctatgcagat gccattgata aattagatga caaagagatt tcttatttga
     7921 actctttcgt cactacacta gtttctgact ggatgaatga ataccatgtc tttttcaaaa
     7981 gaatcaacgc agaacggttt ttcttcgtag cacagatgat cgatctgaaa aagatgatgg
     8041 ataaaaaatt tgatttgctg gatcgctttc gagcagaagc tgaaaaacag agctcgccta
     8101 tcacccttag tatggggatt gcttatggct caagaaccct cgatgaaatc ggtggagaag
     8161 ctcagaataa tttagatgtt gctttagtcc gtggaggcga ccaagttgtc gtgaaagagg
     8221 taaaagaagg agcaaaacct gtttattatg gggggaaatc tgctaagaat gtcaaacgca
     8281 cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg gatttttgat gaaacaggcg
     8341 atgtgtttat catggggcac aagtttccgg atatggatgc tttaggctct gcttttggtg
     8401 tcagttgtct ggcacgcttc aaccagaaaa aagcttacat cgttatcaat gagaacgaga
     8461 tcattccaga tgtagaacgg tgtcttgaag aaatccataa gcatcctgaa ttagaaatgc
     8521 aactaatctc tccagaacgt gccatagagc tgaaaaaaga agaggacctc ctcgtgatgg
     8581 tggattacca taaaccttct ctatcgatct ctcagggcct atatgagcaa tttgataaga
     8641 ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc aagtaaacct ctgctgactt
     8701 atattgaatc ttcagcttct tcagcttcag agctagtttc tgagctgatc caataccaaa
     8761 gcagcaaaac aaatcgctta gatcgttttg aggccacatt attgcttgca gggatggttg
     8821 tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac ttttgacgtt gccagctatt
     8881 taaggtcttg tggagcagac gcatccttag tacaatattt attgagctcg gatttagcga
     8941 catacttgga gatcagcaat ttgattgctg gaagcgacta tgtgacttct gatattgttg
     9001 tggcggcagg gacagacgaa aaagagtata atagtgttat agcagcaaaa acagcagata
     9061 cattgctttc gatgatcaat atcaatgcag ctttcgtcat tacaaagcgg acagacggct
     9121 tgattgggat cagtgccaga agtaccggca cgatcaacgt ccaaatcatc atggaatcct
     9181 taggtggcgg aggacacttc acaaacgcag ctactcaact ggaaaatacg acagttgagg
     9241 aaacaaaaca gaagctattt gctgcaatca aagaaaatat gaatcaaata tatgataagg
     9301 agtaatccaa atgaaagtaa tttttttaca agatgtaaaa ggaaaaggta aaaaagggga
     9361 catcaaagaa gttccagcag gttatgcaca aaattatcta atcaaaaata acttggcaaa
     9421 agaagcaacg aaaagcagta tcagtcagtt agcaggacaa aagaaagcac aagaaaaaca
     9481 cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa ttcttggaaa aagaagatac
     9541 tgtagtcgaa ttgaaggcga aagcagggga agacagccgt ttgtttggtt cgatcccttc
     9601 aaaacaaatt gctgaagggt taaacaaaca atacaaaatc aaattagata aacgaaaaat
     9661 cgaattagaa aatccgatcc gttcactagg ctacacaaac gtacctgtta agcttcatca
     9721 tgaggtgacg gctacgatca aagttcatgt cgtacaagaa taacaaatct tgatagcgat
     9781 gttcagctgg taagtataac cagaaggaaa aaagacaaac cgaaatttat ctgagaaaac
     9841 agctgcagca atgaaagcaa aagctgcaaa tcgatttcag attgatttcg gtttcttttt
     9901 tgggaacagc aaaatagcat aaagaaaacg agtataataa aagagaacta tttttttatt
     9961 acactttcgg tacgaatgat gacttttctc tcattttctt gtaaaatgaa gcaattgaag
    10021 acaaggagcg atacgtatga atgaagtgtg gcaggatcga atcccaccgc aaaatattga
    10081 agctgaacaa gcggttttag gctctgtttt tcttgacgca gaaacgatca ttgatgccat
    10141 ggagtacata gagccaaaag acttttatcg tcgtggccat caaattattt ttgagacaat
    10201 gatcgagtta aatgatcgta atgaagcgat tgacgtggtt actgtcaaag accggctgga
    10261 acaagccaat ttgttagaag atgccggtgg tttaagttat ctctcagact tggcattagc
    10321 agttccgaca gcagcaaata tcgtctacta tgcgaaaatc gttgaagaaa aatcattgct
    10381 gcgtacattg atccagacag caacaggaat cgttacgaaa ggattcgagc aaggagaaga
    10441 agttcaaaca attttagatg atgccgaacg cagtatcttg gaggtttcgg aaaaaagaaa
    10501 tcgaagcggc tttttatcta ttgctgaagt actgaactcc acgattgaaa atatcgatcg
    10561 actagctcaa aacaatgaag aaatcacagg tctaccaact ggctatcaag gtctggataa
    10621 aatgacagca ggtttccagc ccgaagaact aatcattctt gctgcacgtc cagcggtagg
    10681 taaaacagcg tttgcattga atattgctca aaatgtagga acaaaaacag atcgttctgt
    10741 tgcgatattt agtttggaaa tgggggcaga atctcttgta aatcggatgc tttgtgcaga
    10801 aggttcgatt gaggccagcc atttacgtac aggtcagctt tcggaagaag aatggtcgaa
    10861 tctgatcgtt gcgatgggga gtctttctcg agcaaatatt tttatcgatg atacaccagg
    10921 gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta gcgcaagaaa aaggcaatct
    10981 aggcttgata ttgatcgatt atttgcagtt gattgaaggt aacggtaaag agaaccgtca
    11041 acaagaagta tcagatatct ctcgccaact gaaaaaactg gcgaaagagc tgaaagtacc
    11101 tgtcattgct ctttcacagt tatctcgtgg agtagaacag cgacaagaca aacgaccagt
    11161 gctaagtgat attcgtgaat ccggatcaat tgaacaagat gcggacatcg tagccttctt
    11221 gtatcgagat gattattatg aaagagaagg cggagatgac gatgatggaa acgagccaaa
    11281 aaatgacaac gtcatcgaag tcatcatcga gaagaaccga agcggtgcac gaggaacagt
    11341 tgaactactg tttatcaaag aatacaataa gttttcgtcg ctttcgccaa gagaagaatt
    11401 ttaaagaaga ggttgtgagc cttgagcaat cccgagagct ctcgtaagaa gatgcaagga
    11461 gtaaccgcag gaacgactag agtacttaat ttaaacagat ctccaaaggg attgtgacaa
    11521 aagtcttgtc acagtccctt tttccgaata aacggtgttc aatcagctga cccttggtat
    11581 tagctcagca aacgtcaaaa tatgaggaac tattttgacg tttgtttctc taatactcag
    11641 gtcaaaacgc tgatcttacg acctcttttc cttaaacggt gttctcctgc caactcctcg
    11701 gtcacaagtc acaatatcat caaacatgag aagcagtttt ttgaaattgc tccttgtgac
    11761 tcagagttaa acggcaggct cacaacctct ttcttaaacg gtgttcaaaa gcttgcatct
    11821 gcggtaatac gcccaatcaa acgaaaaata tgagaagcta tttttgtttg gctgttctta
    11881 ttacttgatg caggacagct ttttcacaac ctctgattca cggtgttcca aaagtagctt
    11941 cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag caattttcgt acattccttc
    12001 ttatttcttg aagctgacca cttctgttac aacctctttt ttaataacca ttctaaaaag
    12061 ataaagttcg ttttttaagc gtttttataa aataaaaata aaaatcattc gattttattt
    12121 gaaatatgta tctattattg atacaatggt tcaggattaa ataatataga acgaggtgtt
    12181 cacatgtcat ctgtagtagt agtaggtacg caatggggcg atgaagggaa aggaaaaatc
    12241 acagactttc tcagtgaaaa tgctgaagtg attgcacggt accaaggtgg cgacaatgca
    12301 gggcacacaa ttaagtttaa cggtgtgact tataaactgc atttgattcc ttctgggatt
    12361 ttttataaag acaagatcag tgtgatcgga aacggcgttg tagtcaatcc taaatcattg
    12421 gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa caagtaattt gcgtatttct
    12481 gaccgcgcgc acgtaatctt gccttatcat atcaaattgg accaattaca agaagatgct
    12541 aagggcgaaa ataagattgg tacaacgatt aaaggaatcg gacctgccta tatggataaa
    12601 gcagctcgtg taggtatccg tattgcagac ttattagata aagaaatttt tgaagaacgt
    12661 cttcgtttga atctagatga caaaaaccgt caatttgtaa aaatgtttga ttcagaacca
    12721 cttgattttg acgatatttt tgaagaatat tatgaatacg gacaacaaat caaacaatac
    12781 gtcacagata cttccgttat cttgaatgat gctttggatg caggcaaacg cgtgttgttt
    12841 gaaggcgcac aaggtgtaat gctggatatc gaccagggaa cttatccttt tgttacttct
    12901 tctaacccag tagccggtgg ggtaacaatc ggaagcggtg tagggccttc aaaaatcaat
    12961 aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg gtgatggacc atttccaact
    13021 gaattatttg atgaaacagg agaacaaatc cgcgaagtag gacgagaata cggtacgaca
    13081 actggtcgtc cgcgtcgtgt aggctggttt gattctgtag ttatgcgcca ttctaaacga
    13141 gtttcaggta ttaccaacct atctttaaac tcgatcgatg tattaagtgg attggaaaca
    13201 gtaaaaattt gtacggctta tgaattagat ggcgaactaa tctaccacta tccagcaagc
    13261 ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat tgccaggctg gtcagaagac
    13321 attacaggat gcaaaacact tgcagaactg ccagaaaacg cacgtaacta tgtacgtcgt
    13381 atctcagagc ttgttggtgt acgtatctca acattttctg tcggacctga tagaacacaa
    13441 acaaacatct tagaaagcgt ctgggcgcaa atctaaggat aaaaacgacg actgaatcag
    13501 gacgttctta ccagagcgag tcgaagtgtt tatctatacg ataataataa cttgaagagg
    13561 tagtgaaaga gacgtttagc cttattgccg ataggctgtc tcttgaacac tgtttattca
    13621 gaataaggga ctgtgacaaa aaccgtgtca cagtcccttt ttccgaacaa acg
//
LOCUS       DACECZ010000073        13489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.073, whole genome shotgun sequence.
ACCESSION   DACECZ010000073 DACECZ010000000
VERSION     DACECZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.073"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IWO35_001868"
     CDS             complement(<1..132)
                     /locus_tag="IWO35_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0316925.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            541..2913
                     /locus_tag="IWO35_001869"
     CDS             541..2913
                     /locus_tag="IWO35_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316926.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAESGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVTKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFSLGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2974..4434
                     /locus_tag="IWO35_001870"
     CDS             2974..4434
                     /locus_tag="IWO35_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ0316927.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            4445..6649
                     /gene="topB"
                     /locus_tag="IWO35_001871"
     CDS             4445..6649
                     /gene="topB"
                     /locus_tag="IWO35_001871"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0316928.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYHKAAACTSEACDFRLWTTIAKKKLTATQLKEII
                     QNGRTSQPVKGLKGQKGSFEATIVLKEDFTTSFEFSEKKKTNYKKRTRRTTK"
     gene            6895..7491
                     /locus_tag="IWO35_001872"
     CDS             6895..7491
                     /locus_tag="IWO35_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0316929.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYSKAEWYELGKIEIKSRYGNPVIAYDPERTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTIRSYIMALN"
     gene            7504..8403
                     /locus_tag="IWO35_001873"
     CDS             7504..8403
                     /locus_tag="IWO35_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ0316930.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFENRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYEETMDSISNLMEKVI
                     HVEEKERKIAYQRHQQMNREMER"
     gene            8406..8891
                     /gene="ssb"
                     /locus_tag="IWO35_001874"
     CDS             8406..8891
                     /gene="ssb"
                     /locus_tag="IWO35_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0316931.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGILIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            9257..9517
                     /locus_tag="IWO35_001875"
     CDS             9257..9517
                     /locus_tag="IWO35_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316932.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9964..10170
                     /locus_tag="IWO35_001876"
     CDS             9964..10170
                     /locus_tag="IWO35_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ0316933.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            10170..10394
                     /locus_tag="IWO35_001877"
     CDS             10170..10394
                     /locus_tag="IWO35_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316934.1"
                     /translation="MKTQHKLLNVKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFE
                     DLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            10409..10846
                     /locus_tag="IWO35_001878"
     CDS             10409..10846
                     /locus_tag="IWO35_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316935.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEAGVKPSTIKPSEYQALE
                     LTYGKFIETKKAKTVSSEWLDYFENVNKYGACYIMKKEDNENE"
     gene            10839..11546
                     /locus_tag="IWO35_001879"
     CDS             10839..11546
                     /locus_tag="IWO35_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316936.1"
                     /translation="MSNVVYQLTVEDVSHFLTNKEHLNEVVRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDCKRMPYQRFERKKGQ"
     gene            11927..12106
                     /locus_tag="IWO35_001880"
     CDS             11927..12106
                     /locus_tag="IWO35_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316937.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQELKQKYGKSESRIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            12199..12384
                     /locus_tag="IWO35_001881"
     CDS             12199..12384
                     /locus_tag="IWO35_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, HicA family"
                     /protein_id="HAQ0316938.1"
                     /translation="MPLTGKEMLKLLKKNGWVERRQEGSHHHLYKDGVRITVPVHANQ
                     DLGRGLEQKILKDAGLK"
     gene            12418..12849
                     /locus_tag="IWO35_001882"
     CDS             12418..12849
                     /locus_tag="IWO35_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HAQ0316939.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT         4959 a         2282 c         2802 g         3446 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaaagaac
      541 atggcagaag aaaaagaacc actagacaat agtcgtttgg gaaaatccaa aagaaaacta
      601 gtacgattgc aaaatgaatt aaacgaacaa attgaaaaga tgtttgaaca ccaaagaaag
      661 accaacggtc aaccaatgaa cgataagaga aatgggcatt cttggtttag gcaacaagag
      721 cgaatcgaaa ataaagtgca tagtctgaga gaagagatca agcaacaaga aaaacaggta
      781 gaaaaacttg aaagacaaga agagctaaag gaaatgggct ataacaagta cggcggactg
      841 gatatgacga tcgataatat tccaagaatc aaagaagaaa tcgaacgctt tgaaaaaggt
      901 gaatcgactt tttcagccgc cacgatccga aaataccaac ggaagttgga aacattagaa
      961 cagctgaaag aaaaatcaga aaaaggaaaa gaaaatatcc tccctgaagt acaagcgatc
     1021 attgacagcg gacgagtgac gcaatggaaa aaaaatccaa ccatttattt tttgaaaggc
     1081 tatcgaaaag ttgctttaga actgaatgaa aacgggggat ttgaggaatc aaaaaaatac
     1141 caagccacgt cggaagagga aaaagcagct gttcgtgaat tacttgaaga ggctgcacca
     1201 actgattctc tgaaggagaa agaaaaagat catttctatc gagtagaatt caacgaaaat
     1261 gatccagaaa aaggattgaa aagttatggt ggggagatcg taacattaga gttgatcgat
     1321 caacttaaaa cgttggataa ccaatacgct gcagaatcag gttattataa attctatttt
     1381 gaagaagtga agaacggaga agtagtcgat cagatccgaa tggatctagg ggatggcgtc
     1441 aacagtaatc aacccatcta tttagagtta gcaaaattgt gcgaacaagt acctgagaag
     1501 gaacaaaaac aatcggtcaa aaatgaacct gttacgaaag aacaaatgca gtcgaaagaa
     1561 acaaaaacaa aacaaaaggg ctatcaaaaa cgaacggcag aagagattaa acaagaagtt
     1621 aaatcccttt cgcaacaggc actggatgcg gtgaaaaagc atacccattc tcctgaggac
     1681 gtcaaagaac ttttgaactt tatgtctcgt tttccagaac gatcctttag aaatcaatta
     1741 ttgattgagg aacaatttcc aggtgcaacg gcatgtttag gtcgtgccgc gttgaaaaaa
     1801 gaaggaatct atatcaaaaa aggggaacaa gggaacaaga tctttgtcag aaaaacggtc
     1861 aaaggatttt atgagaaagg acgtggcttt gttcgagaaa cagaagctac accagaagat
     1921 aaaaaacgaa tcgaaagtgg tcaaattgaa gtgatcaaaa aaccttacta cacgattgaa
     1981 aaagtgtttg atatcacaca aacacaattg aagcctgaag actatccgaa aatcttcccg
     2041 aaccgcgtct ttgattttca attggataaa acagggcaga cagaacttca agttgggatt
     2101 caagcggtag ccaaaaatat tggaattcaa attcgagata tgacagaaag tgaagtctat
     2161 caaagagaat taggacaagc acgtggggcg tacgttcgta acgaatttac tggcgaagaa
     2221 gaaatcgtca tgaatactcg aaatactcct acgcagcatt tagctacaag tattcatgaa
     2281 ttagcccatg caagaatgca caaattttca gaattagata ccgcaactaa agaattgcag
     2341 gcagaaatga cttcttatat tgtgtgcaaa cattttggaa tggacacgtc ggagaaagct
     2401 attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa ggaagctgcg
     2461 gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat tcaaaccatt
     2521 tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc agaagtgaag
     2581 caggagcata aaaatactgt agaaacggca gaacaaaaga atattgttcc tgttggttca
     2641 ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg aacgttacat
     2701 ttgaaggatg cggcgtttat tgaagatccg cagcataatc aagtgatttt aaaaggaaaa
     2761 agttatggaa aagagattca aacggcgtta ccttcagaag aattttcgct aggaaatcca
     2821 ttccgatttt cacttgaaac agtgtcagaa gtggccaaac gaaaaaatga aatctattat
     2881 gaacaacaat tgtcacaaaa agaacgcgta taaaacaagt aaaaaagact atctgaaaaa
     2941 cggatagtct tttttctttt gggaggtttg aaaatgaccg aaaaaaaaat gagtcttatt
     3001 gatcgatgta aacagatcga tatagtggat tttgctcgaa acaatggaat ggctgtagtc
     3061 aataaaggac gagactatcg cttagaagac catgattcat ttgtttttga tcgaagaaaa
     3121 caacgttttt attggaacag tcaaaatatt agtggcgaca ttattgaatt agctaaactt
     3181 tttttcatag acaaagagat ccaagactct aagcaacaat ttaaagcagc cacagatttt
     3241 attctaaaga atgaagacaa aacagaacga gtagaaaatc ttcattttga gacagaaaag
     3301 tacaaagatc accccgttga ttatcaacca ctcacagaaa aaggaaggaa ttatctgaag
     3361 gaagaacgaa aactaccaga gtggctgatt gattatgcag aaaaagaagg gttgattgcc
     3421 gaactaaagc caaaacatga acggcaaaat ttcttagttc gagacgatcg tttagatcat
     3481 gcggtcgcct ttttatggaa agatccacag acaaatgaga cagtaggggc gagttatcaa
     3541 ggaacgttca ttgattatga acgttttggt gagagaggca catataagca tatagataaa
     3601 aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca gttaaaattt
     3661 tttgagagta gtattgattt acttagctat gctgcactga atcgtgatca gttaaacgat
     3721 acgtggttag tttcgatgga agggttaaaa caccatgtga tcagtcatta ttttggtgag
     3781 gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat ttgtgtggat
     3841 aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc agttgatcca
     3901 tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca agtgccaaaa
     3961 gaatacaaag tcatttatga agaagtcgca aaagaagaaa agatcacacc agaagctatt
     4021 atggccatac acaagacaga aaacaatctg caattaacga accaattggt ttccgctcac
     4081 aaagtaaatg cgtcttttgg tcagcagctt tctgttaacg attcaataga agctattaat
     4141 ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag cttgtgaacg cgtggatggt
     4201 acgtacgatt ttgatcgttt ttatcaagaa aaaggggata ttaatgctca gattcttttc
     4261 tcttataaag cagaacaata ctacaaaggc tataaaaatc atgaacatga atttgtacct
     4321 gaagtaaaaa aggattggaa tgatcaattg aagcatgaaa tctaccaaca agagatcaga
     4381 aagcaaaaac gtgccatgtt gtttcaacaa ggtagacagc aagaaaggga gtgaaatcgt
     4441 catgatgaaa accgttattt tagcagaaaa accgtcacaa gcgaaggctt atgccgatag
     4501 cttttctaaa gctacccgaa aagatgggta ttttgagatc caagatcgat tgttttcagg
     4561 agaaactgtg atcacgtatg gttttggtca tttagtcgag ttagattcgc ccgatatgta
     4621 cgatgaaaat tggaaacaat ggtcattaga gcatttgcct attttcccta cacattatca
     4681 ctatcatgtt cctaaagata agaaaaagca attcaaggtg gtgaaacagc aacttcaatc
     4741 ggccgacacc attattattg cgacagatag cgatcgtgaa ggggaactga tagcttggac
     4801 gatcattcaa caagcaggcg ccgatcatgg aaagatattt aaaagacttt ggatcaactc
     4861 cttagaaaaa gaagccattt atcagggatt ccagcagtta cgagatgcag aagagaccta
     4921 cccaaaattt gaagaagcac aagcacgcca gatcgccgat tggttgatcg gcatgaacgg
     4981 tagcccgctg tatagtttac tcttgcagca aaaaggcata ccaggaagtt tttccttagg
     5041 cagagtccaa accccaacgc tttacatgat ttatcaatta caggaaaaga ttagaaactt
     5101 ccaaaaagaa ccatattttg aaggaaaagc ccaagtaatc gctcaaaatg gcgcatttga
     5161 tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa gcatttgaag attatttaaa
     5221 agaaaagggg gttcaattgg gaaaacagcc aggtacgatc caccaagttg aaaccgagaa
     5281 aaaaagtgcg gcaagtccac gtcttttttc tctatccagc cttcaatcta aaatgaatca
     5341 gctcatgaaa gctagtgcca aagatacttt ggaagccatg caagggttgt atgaagggaa
     5401 atatctaagc tacccacgaa cagatacccc ttatattaca gaaggggagt atgcgtatct
     5461 gttggatcac ttagacgaat acaaacactt tttaaaggcc gaagccattc ctactcctat
     5521 tcatacaccg aatagtcgat atgtgaataa taagaaagtc caggaacatt acgcgatcat
     5581 tcctacaaag acggtcatga cggcagcggc ttttgaacaa ttaagtccac tgcagcaggc
     5641 tatttatgaa caagtgttaa aaacaacggt ggccatgttt gcggaaaagt atacctatga
     5701 agaaacaacg atcttgacgc aagttcagca gcttcagcta aagacagtcg gaaaagtacc
     5761 agtagatcta ggttggaaaa agctgtttgg caaagagagc gacagaaaag aaaaggaaga
     5821 agaaccgcta cttcctaaag tgacaaaagg agaagcggtg acggtagatc tacaagtcct
     5881 tgaaaaagaa acgaagccac cacaacctta taccgaaggt acgttgatca cagcgatgaa
     5941 aacagctgga aaaaccgtgg atagcgaaga agcacaatcc atattgaaag aagtagaagg
     6001 aatcggtaca gaagcaacac gagcaaatat tattgaaact ttgaaacaaa aggaatatat
     6061 caaggtagaa aaaaataagc ttgtggtgac aaataaaggg attttacttt gccaagccgt
     6121 ggaaaaagag ccgctgctaa caagtgccga aatgaccgcc aaatgggaaa gttacttatt
     6181 aaagattggt gagcagaaag gaacacaagc tactttctta gctaatatcc aaaaatttgt
     6241 ctctcacttg ttggaagtcg taccaggaca aatccaatcc actgattttg gatcgacgtt
     6301 acaagaggtg aaagcagcca gtgaaaagca agaagccgcg cgtcatttag ggatttgtcc
     6361 aaaatgccag gaacaagaag ttttactcta tcataaggcg gccgcatgta cgtctgaagc
     6421 ttgtgatttt agattatgga caacgattgc caaaaagaaa ctcacagcga cacagctgaa
     6481 ggaaatcatt caaaatggcc gaacaagcca gccagtgaaa ggattaaaag gtcaaaaggg
     6541 ttcgtttgaa gcaacgattg ttctaaaaga agacttcaca acgagctttg aattttctga
     6601 aaagaaaaaa acgaactata agaaaagaac acgccgcact acaaaataat ggaggggtta
     6661 tcttatgcga aatacaacac acaaacaaca agaaatcgaa aatcaaaaag caaaaattcg
     6721 ttcagaacaa gagaaattac gtgccagtaa aagaagactt cagcaattaa agaaccagca
     6781 acggattcaa aaagaaattg aattagatcg ataaaatctt cctggctatt gtatacgcat
     6841 aaacttatgt tatactatat ttataagttt atacgtatac aaaggaggga gaaaatgaca
     6901 aaagcaatgg tttacaagct gctagaagaa tttaacggaa ctttggcttt gaaggatgcc
     6961 aaaaaagcgg gcatttctcc tgtaacgatc aaacgtatgg tagatcgtgg agagctggat
     7021 cgtgagtatc caggtttttt caccttacct ggtcaatttc ctgatgaatt gtttatggca
     7081 caaaagaaat atgagcgcgg cattatttcg catatcaccg cgttggattt atatgattta
     7141 acggatatga tccctagaca gatcgatcta accgtacctt acggttatca cgtttctgaa
     7201 aaggggttga aagaattcgc agttgagtta cactattcaa aagcggaatg gtatgaatta
     7261 ggtaagatcg aaataaagag tcgttatggg aatcctgtta tagcttatga tccagaaaga
     7321 accttgtgtg acatttggaa tccctggtac aacgttgagg atgaaatcaa agtaaaagca
     7381 atcaaaaact acatggagtc ggacagaaaa aatcttagaa aattgaatga gtatcgacga
     7441 attttgccta cagacaaaac gatacgctct tatattatgg ctttaaatta aaggagaaga
     7501 cacatgacac ctacgcaatt aaaagcaaaa gtgaagaata tttccagaga aaaagaagtt
     7561 gatccgcagc ttgttttaag acattttatg atggaaaaat tcttggaaaa aatctcagag
     7621 tctccttatc gtgaaaattt tgtgttaaaa ggcggcttct taattggctc aaagtatggc
     7681 attgaaaatc gaacaacaaa agacattgat acgactttac gggaaatgaa agtaaccaaa
     7741 gaaacattga caacggttct aaacgatatt ttttcaacgc cgacgaaaga aggcattcaa
     7801 tttgagattc aaggaatgaa agaaacaagg gaagctgatt attatccagg gtttagctta
     7861 cgtgtactgg cacatttgga aaacatgcgg ccagatttta aggtagacgt gacgacaggt
     7921 gactcgatct atcctgcaac gatcactcac agtcataaat tgatgtttga aaatcgaacg
     7981 attcctttgg aatcttatcc tacagaacaa attattgccg aaaaattaag tgcaaccttt
     8041 gattttttaa cagataattc gagaggaaaa gatttttatg atctctatac tattcctaaa
     8101 atggaaaaga tcgatgagaa gaccttgaaa gatagtgtga gaaatacatt tacacgtcga
     8161 ggaaaaactg atccgttaaa gatttattac gaaaaggata tggatgtact gaaaaatagt
     8221 catgagctaa aagatacctg gaagaaatat caaaaaagca atccttatgc tgcgtcgatc
     8281 acgtacgaag agacaatgga ttcgattagt aatttgatgg aaaaagtgat tcacgtcgaa
     8341 gaaaaagagc gaaaaatagc ttatcaaaga caccaacaga tgaacagaga aatggagaga
     8401 taaaaatgat caataatgta actttagtag gacggttaac caaagatcca gatttacgtt
     8461 ataccgcaag tggcacagca gtggctactt ttaccctagc cgttaatcgt aatttcacga
     8521 atcaaaacgg caatcgagaa gctgatttta tcaattgcgt gatttggcgc aaaccagcag
     8581 aaacgatggc gacattagct aaaaaaggca tcttgatcgg cgttgtcgga cggattcaaa
     8641 cacggactta tgacaaccag caaggacaac gtgtgtatgt gacagaagtt gtagctgata
     8701 acttccaact attagaaagc aaagcagcga cagagagccg tgcgcacgct gatcaatcgt
     8761 ccacgagtcc aagcaccacc acttttgaac aacgggatac agcgacgcct aacaacaatg
     8821 gattgaacgc ttcacagaat ccatttggtg gacaatcaat cgatattagc gatgatgatc
     8881 taccgtttta agtagcattt tctattggtt gcatttgcta cggtttatgt tataatttag
     8941 tcaacaaaaa gaattttcaa tctataacca aaaaccacta gctggcgggc tagtggtttt
     9001 cttttacgtt tctattataa gaattaacga gtaggcttac tgtctttctt ggcaaattta
     9061 caatcaagcc atttgcagat atagtgcgcg gctacttctg ccatgattgc aaaaacaaaa
     9121 tcaaaaagaa ttttcaaacc tataacctcc tccccttgcc agattaggcc ggggtagcag
     9181 acagcaagac tattatagca taattgaaat atccaaatga ttggattttt gctataattt
     9241 tagcgaggtg attgtgatga acaaatatgt tggtttgctt gataaaatcc gagtaataaa
     9301 aacgcaaccg ttattggttc gctttacttt acaaacgatt catgaatcga ttaattgtgt
     9361 agttgcagat attgaaatta tcgataaatt gctaattatg gatgatggaa aatacaatat
     9421 tgccgtcaca ggtcacttca ataaacgaaa tcagctggtt attgctagca tgtatgttag
     9481 aaatcccgat cattttacac gatcgatggg catataaata aaagaagagt tgtagtccta
     9541 aatgaggatt gcagctcttt ttgttttaca tagaaacggg aacaggaaaa ggcctgttta
     9601 ggaaggcaca agcgccttct caattaaaaa acctttctct cactccaacc gccaccgccg
     9661 ttcgagaaga aaggaagtga acgctgcacg ttcactaaga tatggtgtct tgcaaagcaa
     9721 gacaaaatag gaaaagcttc ctctcaccaa aaggttcgtt cctgttcgct atctctttat
     9781 tgatttgcca gaccggcaac aagttgcctt ttatcaccaa aaagcgacaa ggacaagggt
     9841 aaatggcacg ctgcgcgccc ttgtccttgt cgcttttcgt tgattcattc tggccaacaa
     9901 atcaataaag aaagaactaa cgaaaggaag taaaaccttt cctaagagga ggcagaatca
     9961 atcatgcgaa agaaatttga ttattggggt gttcccttca gcgaattatt cccaaactat
    10021 catgcaccac atactgtcga atgcgattgt ggagaaagag cgaaatgtat caaaagctat
    10081 cgtttgtatc aatgtccaac atgcgggaaa aaatatacct tgtcttatgg cgattacatt
    10141 ttgattgatg agaaaggaaa gaaacgctga tgaaaacaca acacaaatta ttgaatgtaa
    10201 aagaagtcat ttctgaaaaa gtagcagaag ctttacttat tccgcggtac tggacgatag
    10261 aaaccccttt agtagaggac gcaccgacta tgctatttga agacctagtg aagattgcag
    10321 gatatgaact gcattatcaa gaaaacttgg aaacttatga aaacgaatat tggttaacca
    10381 aattagagaa ctaaaaagga gaaaaaatat ggctcagaat tactatgacg aatttgtcaa
    10441 attacctttg gataaaatgg cacaaaaaat ggaggatatg acgttcctat accatgaaac
    10501 acgtgtaccc aaaaaacatt acaaggaaaa attgtcagtc gcagtggaag aaatgattga
    10561 atcaggtgtc gaaatgaatc tcattgcgac gtactatcga acattggaag aactgaaaaa
    10621 acaaaatgga aaatggtttt tccaagcctt actatgcttg gaagcaggcg tcaaaccaag
    10681 cacaatcaaa ccttctgaat accaagcttt agaactaacg tatggaaaat ttatcgaaac
    10741 gaaaaaagcc aagactgttt caagtgaatg gttggactac tttgaaaatg tcaacaagta
    10801 tggcgcatgt tacatcatga aaaaggagga caacgaaaat gagtaatgtg gtttatcaac
    10861 taacagtgga agatgtttct cactttttaa caaacaaaga acatttgaat gaagtagtca
    10921 gacggattca aaaagacttt agttgtatgg aagaaatgaa gtactttatt caaagagtgg
    10981 aagaacagtt gtatatggaa agcgaaacga gtcaaacgaa ggtcacagtc agccataatc
    11041 atttagacgt gtataaacgt cgtcctgaaa cggtaaaaaa aggaacagag cttctacgac
    11101 tagaaacaga aaaatacatc atgaatgtgt acctagaaag gagtgagctg gtcgaaaact
    11161 ttgtcagcta cacgatttgt aggaaacact attactcaga atcagaatat tattcaattt
    11221 ttgagagcat ggaattaatg gaagaagaaa tggaagagct aaacagcaaa aatgcgacgg
    11281 tagacgatta ttaccttgta tttactagat ttctgacgtg tgggacggaa tctatcgtgt
    11341 taatgggaat ggattatgaa ctcgctgtac ccgaagcatt tttgagggaa tatgtagaag
    11401 aagtagataa cccaggcgac gactggtaca cgactttaac tgatttttta gatacctaca
    11461 cttacgacga tacagaagaa atccaaaatg agcttgactg taaacgaatg ccatatcaac
    11521 gatttgaaag gaaaaaaggt caataaccat ttataccgaa aagtgagaat gtgtgaaata
    11581 agaacaaaaa accttgaaat taagggaatg gttcttattt cgcaccaatc gctccaaccg
    11641 cccccgccgc tcaggtggtg cgaaataagc gggcaacgac cgataaccta agagaaagaa
    11701 ggaaatccat atgaataaaa tcattcctct gttgttcgta ctgtttgttg gcgagatact
    11761 tatgcgtcat gctgcagctg tacatttaaa cttcgtagta ttcttaggaa tactggtatt
    11821 ttgtttgatc ttttggcgat ttgtattagc ccttgtgagt ctactattca agatgattgc
    11881 agccatattt ttcatagggt tatttcgatg aaaggagaga gtactaatgc caattgcaat
    11941 aggaaataaa cgtttacccg tgacgttgga tgaaaagaga caaaaagaat tgcaggaact
    12001 caaacagaag tacggcaaaa gtgaatccag gattatgtgt attgcgttag atttattgat
    12061 tgcacaagaa aaagcagggt ttgatgtacc agcactcaaa aagtgacgtc acttttcatt
    12121 tgaagaaaca aaagtgatag cactttacgc acgtgtcgat acgtgttatg attattttgt
    12181 aaggaggtat agtgcacaat gccacttacg ggaaaagaaa tgcttaagtt attgaaaaaa
    12241 aatggctggg ttgagcgaag gcaagaggga tctcatcatc atctttataa ggatggtgtt
    12301 agaatcactg ttcctgttca tgccaatcaa gatcttggcc gaggacttga gcaaaaaatt
    12361 ttaaaggatg cgggattgaa ataatctcgc attcgattaa agaaaggatt gactgaaatg
    12421 ttaagtgagc ctttagcaaa aacctatcct gctattttta gcccagaaga aggtggtggg
    12481 tactttattg aatttcctga tgttcaaggt gcttacacag gaattaacga agacgatata
    12541 tcttatggaa ttgctatggc acaagaggtg ttaggtatgg ttttagcaga ttatattgag
    12601 catgaagatc tacttccaga accaacacca atcaataaga tttctgttga agatgattct
    12661 tttactactt taattcgtgt agatgttgcc aaatatttga aagacacaga acttgtcaaa
    12721 aaaaccttaa caattcctca atgggcggat aagctaggca aaagggctgg cataaatttt
    12781 tcagtccttc taacagaatc aattgcagat aaagcagata acattcttcg ttctggaaga
    12841 aataattaga actcaaaaga gtcagaaact taaaaatctt tggcttcttt ttttactaca
    12901 aagatggctt ctttaaatta tttgcctgat tgatttaaaa aaattgcgca aagactacga
    12961 gctaattaag ttgctcgtgg tctttcttta tgaaaacttt ttagatctag ataaaaacat
    13021 tgagacactt tgaaaaattt ttttaatagt aatgggcgct tctatgtcaa gctgattctt
    13081 ctttcggtaa attaatttta cttagaatat cactatcttg ataaaaaatg gtgtattcac
    13141 aaattctggc ataaaaaata cctactttct agttaaggtt cctgaataag tcgcaatcca
    13201 attatttcca gctgatttat aactttttaa atataatttt ccataatacc aataaccatt
    13261 acttttttct gtccaatttc gttattgaga agatctagaa ggccagccaa ctttatcaaa
    13321 aatcatcatt cctgtcactc gtatcgtttt agaagctgct cgagtaagta atggagaaac
    13381 tgttaaataa ttttaagtaa cagttaagtc gttagttggg ttatcaatgg ctattccgtc
    13441 cttatctgaa cctcgaaacc caatcataga aaaagttttt tgaacaagt
//
LOCUS       DACECZ010000074        13453 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.074, whole genome shotgun sequence.
ACCESSION   DACECZ010000074 DACECZ010000000
VERSION     DACECZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.074"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..229)
                     /locus_tag="IWO35_001883"
     CDS             complement(<1..229)
                     /locus_tag="IWO35_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0316940.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFS"
     gene            complement(708..1427)
                     /locus_tag="IWO35_001884"
     CDS             complement(708..1427)
                     /locus_tag="IWO35_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0316941.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1476..3122)
                     /locus_tag="IWO35_001885"
     CDS             complement(1476..3122)
                     /locus_tag="IWO35_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0316942.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3301..3891
                     /locus_tag="IWO35_001886"
     CDS             3301..3891
                     /locus_tag="IWO35_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0316943.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3983..5488
                     /locus_tag="IWO35_001887"
     CDS             3983..5488
                     /locus_tag="IWO35_001887"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ0316944.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5572..6924)
                     /gene="celB"
                     /locus_tag="IWO35_001888"
     CDS             complement(5572..6924)
                     /gene="celB"
                     /locus_tag="IWO35_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0316945.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6924..7442)
                     /locus_tag="IWO35_001889"
     CDS             complement(6924..7442)
                     /locus_tag="IWO35_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316946.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7458..7784)
                     /locus_tag="IWO35_001890"
     CDS             complement(7458..7784)
                     /locus_tag="IWO35_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ0316947.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7797..9776)
                     /locus_tag="IWO35_001891"
     CDS             complement(7797..9776)
                     /locus_tag="IWO35_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0316948.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9797..10111)
                     /locus_tag="IWO35_001892"
     CDS             complement(9797..10111)
                     /locus_tag="IWO35_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ0316949.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10279..10572
                     /locus_tag="IWO35_001893"
     CDS             10279..10572
                     /locus_tag="IWO35_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ0316950.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10650..11762)
                     /locus_tag="IWO35_001894"
     CDS             complement(10650..11762)
                     /locus_tag="IWO35_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0316951.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11915..12139
                     /locus_tag="IWO35_001895"
     CDS             11915..12139
                     /locus_tag="IWO35_001895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316952.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            12149..12328
                     /locus_tag="IWO35_001896"
     CDS             12149..12328
                     /locus_tag="IWO35_001896"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316953.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12522..12692
                     /locus_tag="IWO35_001897"
     CDS             12522..12692
                     /locus_tag="IWO35_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0316954.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(13116..>13453)
                     /locus_tag="IWO35_001898"
     CDS             complement(13116..>13453)
                     /locus_tag="IWO35_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0316955.1"
                     /translation="GLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEK
                     EQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFIS
                     IKFRKKRNI"
BASE COUNT         4259 a         2706 c         2288 g         4200 t
ORIGIN      
        1 aagaaaataa aaatttgaaa ttttcccaac ccacccaagg actttccctc aagctagcca
       61 agaacccatc tgctgaaata tgaaaatttt tgaatgctac gacattcgcc attacaggta
      121 tgtaaaagaa aaaaatcatc caaagcatac ccggtacagc catcaggaca agtgctttat
      181 aacgccatag attcataaaa aaatgattga acccctttcg ttttttcatc taaaaacccc
      241 tttcgattca ttcgcaacgt aattatagcg ctttcaataa tgagaataaa taaacaaagt
      301 atagggaaaa tgaaacaaag tataggagag gttgtgaaat cagcgttttg gcctgagtat
      361 tagagaaaaa aacgggaaaa tagttcctca tattttgccg ttttttgagc taataccgag
      421 ggtcagctga ttgaacaccg tttatcagag gttgtgaatt cagcgttttg gcctgagtat
      481 tagggagatc gtgtccttac tatctaactc tgagtcacaa ggaacaatgg ggactgtaac
      541 aaaagtcttg tcacagtccc ttattccgaa caaacggtgt tccagaagta gcttctacgg
      601 aaataagccg aaatttcaca aaaaagccat ccgaaagtca gacggacaag tctgcttttc
      661 agatggcgct acaactactc tgcttattct tttagctatc cattcaatta tttttctctc
      721 acttgttcta gctcttcttt ggtcatgggt cgatattgcc ctttattcaa atctttaggc
      781 agccaaaatt ttcccatacg gattcttttt aaatataaga cttcttttcc tactgctttg
      841 accattcgct taacttggtg aaactttcct tcatgcaaaa tcagacggat aattgaagtt
      901 tctgtctctt gatctacttg atcaatttgg agttttgccg gcaatgtctg ctcatcgcca
      961 tcaataatca gtccttctgc aaataaacgg atatcttctt ctgtcattat accgctaacc
     1021 tcagctagat attctttctc cacatgtttt ttaggtgata gtaactgatg ggcaaacacc
     1081 ccgtcattcg ttaaaatcaa gaggccctct gtgtctttat ctaatcggcc gactggaaaa
     1141 agatcttccc gataatcctg gtcttccaat aaatcgatta cggtttgatc atgattatcc
     1201 tgagtagcag aaatgactcc tgcaggttta tgcaaaatgt agtaaaaatc tttttgatac
     1261 gtaatgattt ctcctaaata agtaaccgca tcttcgaatt catttacttg cgctttatcg
     1321 cttttcacca cttgtccatt gacttcgatc gttccttttt tgattaatcc tttgacttct
     1381 ttgcggctgc caagtcccat atctgctaaa aacttatcta aacgcatgcg tttcccccat
     1441 ttcaaaagag catcaatcta gctcttctga ttcacttatt ttatttttaa tttatgtcgt
     1501 aaacgcgcta ttcctggtcc tagtagtctt tctgccaagc ggattttcaa agctaaataa
     1561 ccatacacta aagcccctat acctgctact acaagaatca acaatagaga ctggaatttg
     1621 ctatcttcgt ttaaaaaagc accaaaaatc atctttgtga tccacgaagc caaaagcata
     1681 atcaaggtaa gaataaagat cagcaatgtc ctgcgccaga caaaacttgg tttaaagcgg
     1741 gctacttcat ggattctctt taagatcaag gtacaagaca cagcaaatcc gatcattgtc
     1801 gccaataaag ggccgtatac ttcaaataaa cgaatggctg ggtattgaag aaggagtttc
     1861 actagaaacc caataaccaa ataattaatt gccgccttgt tttcaaacat tccttgcagc
     1921 atgttggaaa ctagcatata tagtcctagg aagagtccta caaaacttgc ttggatcagt
     1981 acattacttc ctagagaatc cggtgtataa aataatgtat ttagcggata ggcaagtaac
     2041 ataacaccaa atgtcgccgg aaacataaaa aatgaaaaca actgtaaatt tccgcttgtc
     2101 aattttgcca gacctactcg atctttcaac gtaacagctt ctgtaatcaa cggtaaccca
     2161 gtagacgcga tcgatgttgc caatgcaata actaccatcg tcaacttatc tggattggca
     2221 ctaaaaatcg aaaaaagatc cagtaactgt gcattggaat actcggtgga tgcagacatg
     2281 atcttcataa aagtgaactg atcgactaat ttgaagatcg ttacccctga acccacaata
     2341 ataaacggaa tcgcttcttt aacggtatct aacaacaatt ccttcgttgc aatatttatt
     2401 ttgttttcgc tgtagtgaat aaacgccgat gtatacgctt gatgtttctt caaaaaatac
     2461 aaaaggacaa gaatacttgc tagcataccg ataaatgccg cgaaagttga ttgcgtgacc
     2521 gccgtcacat agtcaccatc catgactttc atgatgatga atgtagccaa tagcatataa
     2581 aaaatacgtg ccacctgttc tacaatctgc gacagcgcat aaggcatcat ttcttgattg
     2641 ccttgcaaat accctcgtat cacactcata caaggaaaaa ccaatacagc gatactcaaa
     2701 gaacgcatcg ttggaatcag ttcttctccg ccaccagaag catgagctag ccaaggagag
     2761 gcgatgtaca tgaacaaagc aaacaacagg cctaacccgc ccatcatctg taatgcacgg
     2821 ataaataatc gtcgtgaagt gccatattca ttcaatgaat tatagcgtgc agtttgtttt
     2881 gcaatcgcag caggtattcc tgctgtagag atcagtagaa acaatgcata tatattgtat
     2941 cccatattga ataatccatt ggcagcctta gcattttcgc ccatccaagc ataccaggga
     3001 atgatataga cagcgcctag caatcttgaa atgatattac tggcggtcat ccaagcagag
     3061 cctcgtgcca ttttttcttg cggtgttaat tgatctaatg ctggttttct ttgattatcc
     3121 atcggtcaga ttccaacttt ctacaaaaca ttactatcat tttaattggt aaaacagaaa
     3181 ctggcaatcg attttatata acttttataa gaacaattgt ttttttgaat tcggtttcgt
     3241 ttttttggta aagtaaagaa atatacagcc ttttatggct aacatttata ggagatatca
     3301 atgacacaag aaataaaaaa actacccgtt atatttttag gtcttttctt tatcacagtt
     3361 ggattgaatt ggttcttgct ccctcacgat atagcttccg ccggagtggg cgccatcgga
     3421 catttagttg aaacggcctt ttctatccaa cgctcagtta ctgtttggag catcaatctt
     3481 atgatgctgc ttctagccgc tgtcctatta ggaaaatcag tattttttaa gactgtagcg
     3541 ggaagtctgc tttttcctgt tgttttagag attgttccaa aagtggaaat gatctcctca
     3601 catttctttt cactgatttc aggaagtctt ttgttcagtc ttggggttta cacattgtac
     3661 acagtcgggg cctcaaatgg cggtataacg ataccgccga ttattttcca caaatttttt
     3721 cggcttccaa tgccgcaagg acttttactt accaatagtc ttatcgtctt tttgaattat
     3781 tatgtttttg gattgttgga gacactattc gttctcctat ctattacagt cagttcattg
     3841 tttatgaaaa tactcatccg cttgtcccct gtgtcaagaa aatttgaata aagctggatt
     3901 ttgtcatagc ggaaacaaac gatatattaa aaagagtgtg ctataataaa ccaagaaatt
     3961 gaataaagcg agggatcaat caatgagctt ctctttagaa gacatccgtc atttactatt
     4021 aaaagaaaat ctattaaaag aatttgtttc aacaagaggc tggcatcttg atgtaccaga
     4081 aaaagctgaa tttcttcagt taagttatga ttcaagaaaa gctgatgctt ctactttgtt
     4141 tttctgtaaa ggaatgaatt tcaaagaaga atatttggat tcagcaatcg aacagggtat
     4201 tcaatattat gtatcagaac aaccatacga aaattcagca gtaggaatca tcgtaacgga
     4261 tatccgtaaa gcaatggcgc ttcttgctat ggctttttac gactatccgc aaaataaatt
     4321 gaaagtgatc ggattcactg gaacaaaagg taaaacaact gctgcgtact ttacaaaagc
     4381 aatcttggat cacacaacaa ataaaaaaac agcattgctt tctactatga atacaacgtt
     4441 agatggcaaa acatatttca aatcccattt gacaacgcct gagtcattag atttatatcg
     4501 tatgatgtca gaagcagttg aaaatggtat gactcatcta gtgatggaag tttcttcaca
     4561 agcttacaaa acacaacgcg tatacggctt gactttagat gtcggaatct tcttgaatat
     4621 ctcgcctgac catatcagtc cgatcgaaca tccgacattt gatgactatt tctattgcaa
     4681 gcgacaattg atcttgaatt caaaaacagt tgttttaaat catgaaagtg actattttga
     4741 tttacttagg gaaacagccg atctattcca gattccaacg atcacttatg gtcgttcgga
     4801 agaaagcaat tatcaagtga tccgttcaga taaaggtact cacggtttta ctttatcttc
     4861 tcatgaagat cgcttagcta tctgcgatac tgcttatgat attttgctgg ctggaggatt
     4921 caaccaagaa aatgcagcca gtgcgatcat tgccgcagca ctaacaggtg cctccagtca
     4981 agatgcacaa gacggattaa aagaagctcg tgtaccaggc cgaatggatc aattgatcca
     5041 atccaatgga gcgcatgtct atgtggacta tgcacataat tatctaagct tgaaaacatt
     5101 gctgaaattt gccaaaaatg aacatccaga tggccgtgta attgttgtat taggcagccc
     5161 aggaaacaaa gcaatctccc gtcgacatga ttttggaaaa gtgctatcag aaacagctga
     5221 catcgccttt ttgacggcag atgacccagc atttgaagat ccgaaaaaaa tcgcggaaga
     5281 aatcaatgaa gcaattacta atccagattt gatcgttcat tatgaaatgg atcgtcctga
     5341 agcgattcga cgcgctttag cagaaagcgc tccgcaagat tcagtcgtta ttgcgggaaa
     5401 aggcgtggat ccctatcaaa aaatcaatgg tgtagatgaa ccttatgaag gggattatgc
     5461 aatcgtgaag cggttgattg aagaataatg gtatttatat actagtggtt tcccatttct
     5521 aagaaaaaaa gggactgtga ttttttgtca cagttccttt tttcttagaa atcattttaa
     5581 cttttcacta gctaattctg ctgccttttc tctttcttca tttaataatt tattatcata
     5641 gaatttgata aatgggaacc agataatgaa tgcgatgatt gcacaaatga ctgctaacac
     5701 agcagctttc caatcaccac cactaccaac aaatgctccg atcccgactg gtgaaggcca
     5761 cggcatctgt gcaagcatcg gtttgacgat ttccagccga atggcaaaat aagcgagtgt
     5821 agcagaagcc atcggagcta agaaaaacgg aaccgctaaa tagggattgt agacgattgg
     5881 catcccgaaa atgattggtt cattgatatt gaataatcct ggtacaagtg aggcttttcc
     5941 taacactttc agctgatcag atttcgcgag gaatgcaata aagatgatca atcctaaagt
     6001 cgcacctgat ccaccaacgg ttacataaga attgttgaat tctcctgcca acggataatt
     6061 tgcgccttgt gcgttagctg ccatattagc taatacgatc ggggtcaaga aagaagtgat
     6121 gatattcgct ccatggatcc ctacgatcca caatgcatgg atcaagaaat aaatcaccat
     6181 gacaccaagc catgtattcg tcaactgcgt aacaaaccca aatgggatag caatcatttt
     6241 gaagatatcc gttcctagtg caactaataa tccattgatg atcatcacca caaacgcaat
     6301 gacaaatgtt gggatcagag cagtgaaaga acgagaaact cctggcggaa caacatctgg
     6361 catttttatg acccaatttc tcttgacaca catacaatac aattcgaccg caataatcga
     6421 cataataata gctgtaaaga ttcctgaagt tcctaaacgt tcgacaaaag agcccatcct
     6481 aataccattt actacagttt ctgtctcatt gatagaagca atcaagctga ttttaccatc
     6541 agtcatgatc aattcaggga tacacataaa gaatgcaaaa acagatagta atgcccccgt
     6601 gagcggatta acatttaatt tttgttcatc tcgttcaatt gacgttaatt cataagcaaa
     6661 cacaatggca aagtaaatag ataaaatccc catcgtcatt gtattgacga tcatgtacaa
     6721 gtcactaact ttgaaaaaag ttgcattaaa aaatccttct aaaaatggga acggtaatgg
     6781 caaaatattt aaaaccaaaa acatcgagcc tacaatcgtg aaaggaatag aagccatccc
     6841 cgctgccatg acagcccgaa cgattttaag ttgcgcaatt ttccccatag gtcccatcag
     6901 gtatttctgc aaaaaatcaa acattagtta ttctcctttc ctaaatataa cgctttttca
     6961 tattgtttaa tacggatata ctgttggtct tggttgagtg aaattttctt taaacgatac
     7021 tgtataaatg cactggcaaa aataccgctt acaacaagaa tgaaaacaaa taactgtcct
     7081 ccattgttat ttgccataaa aggatacaag aatgtaaatc ctgaggtaaa caacactgga
     7141 atcactaata aattaaagac actctggaac aaaaaatacc actttgtcca tggaacgttc
     7201 gtttggtgtt ttcgataaag ctttacttgt tctcctactg caggcaaaat agctaaaatc
     7261 agaatcatcg gtaaactact tcctaaactt ttagctgata gaagcagaat catccattgc
     7321 atgttcatga aaaagaaaaa ggcagtcagg taacgtacga ttagataacg attgaaatac
     7381 atgtttttca aactaagttg ctttttttct aattcgattt tcctttttac tgtatcctgc
     7441 acaatgatcc ttcctttcta tttatttttt tgatacaaat aattcatttc ctgtgcaact
     7501 tccaataatg tcatcgtcgt catcagatga tcttgtgcat ggaccatgat aatttccatt
     7561 tctatttttg tcccgctggc atattctttc agaagttgag tttgtgactg atgcgcatga
     7621 agaatctctt catttgcttg tcgcaacttc tcttctactt cttcaaacat ctctttacgc
     7681 atcgagcgaa atgcttcatg tatcagtgtc cgtgcatttc cgctatggaa aatgatatca
     7741 aaagaagtca cttgtagtgt ttcactgttt attttttcag ccatctgttt tcctccttat
     7801 tcgatcagtt tgataaattt actgctgaaa ttctcaaaaa taggttcagc taagatttcc
     7861 tgctgtactt ttagctggtc aactaaccta acgattgctt tagtcacgac cgtaattcct
     7921 tcattttcga cataagaagg cgaaactaaa aaaacaaaat gaatttcttg atattgatcc
     7981 cactgcatcc cagaaggaat caatgcaacg gctacctttg tttcgattcc aactggaaca
     8041 gcgggatgtg ggactgccac tgtatcactg aatatgattt gtcccatatt ttcacgttgc
     8101 tggatctgct ttcgcatttc tcttatatat ccttcttctt catttatgct cagcttttgc
     8161 agcaaatcat gaagaatttc ttcctttttc ggttcgtacg tataagtctt gaaaaatttt
     8221 tcagacaact gttcggtgta aatctgcttt ttttgctgta gagaaaatgc tggtatcggt
     8281 ctatctgagg aatgataggg tatagattct ccaactacct ttcgtatttt acgaacatca
     8341 tcttcattca aaaaaatact gacatgtaaa actggaattt tgaaaaacat tgtcgaaaga
     8401 tcaatagaag aaataatcag gtcgattcct tttaaagttg tttcattgat ctcataatag
     8461 ccttttacac ttaccactgt aatattttta ccgaattcac tgactacgcg attctttaat
     8521 agttgggcac tgccgtaacc cgttgcacag ataatcaatg catgtagttt atgcgcagct
     8581 ctttcttttt ccaatgccgc catgagatgc aaagccaaat aggcccattc atcttcattg
     8641 atggtatagt ttttcaaagc cggcatgttg ctgaaatgcc tttttactgc gtcaaagact
     8701 ttgggatttt ctttctttat ctcctcagtc aatgggttct caatgcgaat acctcggctc
     8761 aatcgaatca tcatcggtga caaatgatcg agtagtccat tgaaaagctg gagatcctct
     8821 actaatgaaa aatgaaattc ctgagacaat cttttcagaa ctttcgctaa ttcctttgat
     8881 agttcctgat cattttgatt ttctttgtga ttcgacttag ccatgagatg taatgtcaaa
     8941 taagcaattt cttcttcggg aaacgataca cctgcgatta cctcaatacg ttgaacgatc
     9001 cgctgcgcca cttgatactc tactcgctta gaaacttctt gttcgatgcc taattccttg
     9061 atttcaaacc cttctcggag ccgtttgatc cccaaagcca agtgtaaagt cagattctgt
     9121 atgataaaat ccgaaacttt taacttagct tctcttgttt catcgagaat gataatgact
     9181 agctcttcaa aactgatgtc tgggaaaaaa cggctttgcc ctaaatattc tttcaatgaa
     9241 tttccataat ggtttccaaa aaaagtatcc atgataaaat gccgtcgatt tctttcttcc
     9301 ccttcgatcc atgtcccttg accatgctta gaaacgattt ttagtgaata tggaatcaat
     9361 cgctccttga tttcttgcat gtcctttgtc aaactggaac gactgatata aagctcttct
     9421 gccaaatcat ccataaaaag aatggcatct tccaaaagca attggttcaa tatatacttt
     9481 tgtcgatctt cggactcata aaatccttgc ggttctctag catatccagg tactatttct
     9541 tgccgactca aaaacaagtc aaatgtcagt ctttgtaaaa tatgcagttg gtagccataa
     9601 cctggtttag caatgatctt acctccgttt ttttctacta actctttgat ccgctgcaag
     9661 taattacgga ttgtccgatc agataataac aattcagaag ctaaacgttg actggtgaca
     9721 tactccttct tgtgatcaat caaatatctc aacagcaatt tttccttctg tttcattatc
     9781 atccactcct attccatcat atgttctctt cgatcacctc tgctaacttc tggatgcctg
     9841 tagggattgg gacatacgct tggaaaggga tactaacaac aggctttcca acagattctc
     9901 ctaatttttt gaatttatcc aaaaacatcg tcgtttgcgg gctgatcaga aataaatcaa
     9961 aatcactatt cttaatcata ttatcgcctt ctgtagctga aactgcatct acgagtacct
    10021 cttctctttt ttgcttaaag aaatccgttg tctttgaggc catcatcgag gatgacattc
    10081 ctgccgcaca aataatcaat gcttttttca taaatcaatc actcctttta tttttataaa
    10141 ccaagcatat cttgtaagcg ttttctattc aaaatatata tttccgtagc attgcggaaa
    10201 aaatgtgtat catatcttga ataataattt ctggcaccgt gatatactag tgatagaatt
    10261 ttttcaggag gggatttagt gagtatcaca gtgaatattt tgtatacaag tacaaatgga
    10321 caagccagaa aatttgcaga agaaatggtc gaaagaaaca ttgtcaaagc gattcgtgca
    10381 gaagaaggaa acgagaaata tgactattac tttccactag aagatccaga atctctttta
    10441 ctgatcgacc gttggaaaga tgaagaagca atcgaaaagc atcacaaatc cgaaatgatg
    10501 gctcaaataa acgaactccg taaaaagtat aaattaaaaa tgaaagtcga acgtttcact
    10561 gatttaccgt aaaaagatac acatggaatg atagcaattt atcatccatg tgtatcttca
    10621 ctttttcctt attttttatt cgatctcatc taatgattca aaccgtgttt ttctcttatt
    10681 tttcggataa gtatttcagt caccccaata tacatgattc ctaaacatac cccaaggata
    10741 ttctcaaaaa tgcggatcgt tactgctcct ggaaccccga ataaactggc agtgattgcc
    10801 aaagcaccaa aagaattaaa aactgtttta tatctatagg tagaacaaac acctaaagct
    10861 agaccaccta atataccgac ccagttaaaa ggaataaatt ggctgatacc aataaataaa
    10921 acacatccaa tcaatgagcc aatgattcgg tctactgctc gttctttgaa accaatcgac
    10981 atcaatccat agctagatac aatagaagaa aatgcaaacc ctgcccacat caatcgctga
    11041 aaagggacat actctcctgc aacgatcaaa agactaatgc caaatgcata gatgagcatc
    11101 caaatttttt cctgcgaata catgcctttc aaaaaaatct ctttaaacaa gcttttgtct
    11161 ttgttctttt ctcgatgttt tctgtaaagg agaactgaaa accaacaaaa aaacaaaaat
    11221 aataagctac ttgtttgtgt aaagaaattc ttgttcaacg aatctttagg taacgaataa
    11281 acaataaaga gatatgaaaa gccgtacaaa gaagcattcc ccattttggg atcactagcg
    11341 gttgccatta atatgctgct tagtaaaata aaatgaatca acatgcccat aactgaccat
    11401 ttgatcagct ggacatacgg tgctactaat agtgagaaca tgacgattgc taaacctatg
    11461 atcgaatggg aaatcttata accaaagtca acaaaacgaa tactcatcaa catacagaat
    11521 aaaaccacag cgtatggttt atgcttcgat tcaaataatg ttgtaattat aatgataaaa
    11581 caaatggcaa acaatacgat gaataatgat cgtaaaaaca atgaacaaat atacatgcac
    11641 ttttcttttt tagaagctgc ttctcgtatt ttttgtttta aaataaacgg atctagttgt
    11701 agagactgat aaaaatccac ctgttgtcgt cctttctttt tacttttatt ttttacagtc
    11761 atctatgaca ggttgtaaat actatggaac atcatatggc gttgtcattt tcttgtcaac
    11821 tatttttcct attcatgttt ttataaaaaa acatatcgtt ttcttgtatg attaaatcac
    11881 cacaaaataa acaactgaga gaacgatatg ctttatgaat ggttctatca aaaaaattct
    11941 gagatcaata gacaaaaatt tgatgattac agaggtctct tacgaaacct tacagaagaa
    12001 aaaaacattg atcgttgctg cagttctctt ccctgctcca tgtgtctgta gaaattgtgg
    12061 ttctgctgtg gtagatggaa ataggaaatt aatggtggtg aaaaacaaga agaaggaaac
    12121 cattgtccac tttgaataat ataatcatat gccaatggtt atacgcctaa aaaagcagcg
    12181 ctatacctgt aaaaactgtc gaagccattg gaacgctcaa agttatttta tccgtccaag
    12241 gcactcgatt tcaaatcatg tcagacataa aatcacttct ttactgactg aaaaagtgtc
    12301 gttatttttt atttcgaaaa gttgttaggt atctttgaca accgttattc gcacattgaa
    12361 agagttgaga agctatttac caaagtcttc caaaagaatt ctgtcttgag tgttgagggt
    12421 tgtgaatttc gttctcacgt ttccgttgag gataaactga gttttatttc cgcggatggc
    12481 gagacagaaa aattaatcga gaaaatttac ctacacaaaa aattcctcga ttaacaaact
    12541 atttccttag ctgcactaat cctaaagaaa tagaattctt agttacagac atgaacgcca
    12601 cctactttca gctcaccaaa catgttctac caaatgcgaa attggtgatt gatcgattac
    12661 ttggtttttc cacgcctttc aaggaggcgt aacctttatt cacgagttgg ttgaagcttt
    12721 ccgagacaaa agacccgact tgtttttctc cttattgaca aagagccatt aaaactagag
    12781 aaaaaattac aactgatcgc tgattatttg tagaaagaaa atcaaagtat tacccaataa
    12841 acttgagtca ttaggcaaaa aacactgcga aactcaagaa tgattttcgc agtgtttttg
    12901 tatatttttt attaactagt attactacaa aggtttattt ttcacttctc aacgcagcac
    12961 aaaattgtct ttacttattt actggttggg ttcaaccttc tctactccga tactaatatt
    13021 tgtttgtata ccccttctaa aatcggtagt ttccattcaa ttgatgggta ctagttgatt
    13081 ttaaaaacta tgcttattca attttttatt ttagctcata tatttcgttt ctttctaaat
    13141 tttatactga tgaaaacaag tagcacaccc aatattgaca atataaactg gctaatgatg
    13201 gtttctccag tttttggaag atgcttattt ccactgcttg ttggattgtt tcctcttata
    13261 tctgagctat cagcaacttt tctattctct tttgttaaaa agatcgccac ttcttgttct
    13321 ttttcgatct tgaaagttac tggtgtcgca tccaattcat accccgttgg cgcttgagtt
    13381 tctaccagct gatatgctcc tggtgctaag ccatctatcg ccagttttcc atctgctcct
    13441 gtggttagac ctg
//
LOCUS       DACECZ010000075        13127 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.075, whole genome shotgun sequence.
ACCESSION   DACECZ010000075 DACECZ010000000
VERSION     DACECZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.075"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            162..1103
                     /locus_tag="IWO35_001899"
     CDS             162..1103
                     /locus_tag="IWO35_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ0316956.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1172..1903
                     /locus_tag="IWO35_001900"
     CDS             1172..1903
                     /locus_tag="IWO35_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ0316957.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2047..4227
                     /locus_tag="IWO35_001901"
     CDS             2047..4227
                     /locus_tag="IWO35_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ0316958.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4247..4729
                     /locus_tag="IWO35_001902"
     CDS             4247..4729
                     /locus_tag="IWO35_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ0316959.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            4745..5506
                     /locus_tag="IWO35_001903"
     CDS             4745..5506
                     /locus_tag="IWO35_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0316960.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            5637..6971
                     /locus_tag="IWO35_001904"
     CDS             5637..6971
                     /locus_tag="IWO35_001904"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ0316961.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7278..7763
                     /locus_tag="IWO35_001905"
     CDS             7278..7763
                     /locus_tag="IWO35_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ0316962.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            7756..8439
                     /locus_tag="IWO35_001906"
     CDS             7756..8439
                     /locus_tag="IWO35_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ0316963.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8565..11210
                     /gene="polA"
                     /locus_tag="IWO35_001907"
     CDS             8565..11210
                     /gene="polA"
                     /locus_tag="IWO35_001907"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ0316964.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11255..12091
                     /gene="mutM"
                     /locus_tag="IWO35_001908"
     CDS             11255..12091
                     /gene="mutM"
                     /locus_tag="IWO35_001908"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ0316965.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12055..12684
                     /locus_tag="IWO35_001909"
     CDS             12055..12684
                     /locus_tag="IWO35_001909"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ0316966.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            12736..13068
                     /locus_tag="IWO35_001910"
     CDS             12736..13068
                     /locus_tag="IWO35_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0316967.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
BASE COUNT         4429 a         2321 c         2896 g         3481 t
ORIGIN      
        1 ttttctgtgc gaaacaggaa agcgaagtct ttttaattca aataataata ttatgtaaac
       61 aaaaaacttt ccatgcgtaa ttatacacaa gcaaataaat cgtcaaatgc gaacgccttc
      121 tttatactgt ttaggaacga aaagaaggaa ggaagaaata catggattat caagtactgc
      181 tttattacaa ttatacaaaa atcgaagaac cagaggaatt tgccaaaaat catttagcct
      241 tttgcaaatc tttggatctt aaaggtcgta ttctagtagc tgaagaagga ataaatggta
      301 ctgtgtctgg aaccgttgaa aacacgactg cctatatgaa agcaatgaaa gctgatcctc
      361 gatttgctga tacgatgttc aagatcgatc caagcgaagg acatgccttt aagaagatgt
      421 ttgtaagata ccgtccagag cttgtaagtt taaagctaga agaggacatt gatccaaacc
      481 aagtgacagg tgcctatctt tcaccgcaag aatttaaaga agctatttta gatgaacaga
      541 cagtggtgat tgatgcacgg aatgattatg aatatgactt gggacatttt cgaggggctg
      601 tacgcccaga tatacgcagt tttcgagagc ttccagactg gatccgcgag cacaaagaag
      661 aatttatgga aaaacgggta gtgacctact gtactggtgg cattcgttgc gagaagtttt
      721 ctggttggct tgtccgtgaa ggattcaaag atgtcggaca gctgcatggc ggtattgcta
      781 cttatggtaa ggatcctgaa gtccaaggag aattatggga cggcaagatg tatgtgtttg
      841 atgaacggat cgctgtcgat atcaaccaag tagataaaca aatcgtcggt cgcgactggt
      901 ttgacggaac accatgtgaa cgatatatca attgtagcaa tcctgagtgc aatcgtcaga
      961 ttcttgcttc cattgaaaat gaagaaaaac atttaggagc atgtagcata gagtgtgcga
     1021 aacatccgcg caatcgctat gttcaagacc atggaatctc tccagaagaa gcagaactgc
     1081 ggtttacagc tttaattaaa tagaaaacaa tagttttgat tgtcgaccat ttccaatatg
     1141 cgctatacta gcgttgagga tggtgatcca catgaaatca ttgatttatg ctattgggga
     1201 tgtccatggc aaacatgagc tttttgaaaa acttattcac tattatgatc caaagatcca
     1261 tcagcttgtt ttattagggg atctcaatga tcgaggtcct cgtacaaaag aatgtttttt
     1321 attaggaaag caattagtcg aagaaacagg agccatttac ctccggggaa accatgaaga
     1381 atattttttg caatttctgc aatcaccgga ggactggtat gcgccttata tgagaaatgg
     1441 tggaaaagaa acgtttgaaa gcctgattca cccaggggca tcggaggaat actccccaac
     1501 tgaaatctcg atgatgatcc aatcgagata ttcagaactg attaaatttt tagtccagcg
     1561 ccccttgtat tatgaatggg aaaattatct ttttgtccat gccggtgtgg atttgacaaa
     1621 gaaagactgg cgtaagacaa gtctaagaga ctttatttgg ataagggaac catttcatga
     1681 agggaaaaac aatacaggta agacaattgt atttggacat acgattacac caatgctcca
     1741 tggagatatg cagacaacag acctctggat tcaggatcat aaaatcggaa tggacggtgg
     1801 ggctgtattc ggagggtcat tgcatggggt tgtttttgac aaggaaggta ttcttcaaga
     1861 tattgaatat cagaacaacg aaggaccttg gcatggaatc tgactaatgg aaagaatgaa
     1921 ggagctgtga ctttgttgcg gctcttttta tttttataga gaatattgtt tctttcagta
     1981 gtttaaccca ggataaaaag tatgatagaa taaagaagca atttttagca gaaaagaggc
     2041 attttaatga cggaaaattt gaaccagacg atcattcagc ttgtacaaaa agaactttca
     2101 gactatcgtc ctaagcagtt gactactgta cttaatttgt taaatgaagg aaacactgtt
     2161 ccttttatcg cgcgttaccg aaaagaaatg actggaagtc ttgatgaagt ccagattcgt
     2221 gaaatcgaag agcggtatgc ctatcttgaa aacttagaaa aacgtaaaac tgaagtgatt
     2281 cggctaatcg atgaacaagg gaaactgact cccgagttag aagcagaaat cacccaagcc
     2341 gtgaaaatgc aacaagtgga agatttgtat cgtccataca aacaaaaacg acggacaaaa
     2401 gcaacgattg ctaaagaaaa aggacttgaa ccactcgcga agtggctgat gcagcttaca
     2461 gatggagagg tgcaatctga agcagaaaaa tatattgaca aagaaaaaga agtctcttct
     2521 gccgaagaag cgttgcacgg tgctcatgag attattgctg aacaagtcag cgataatgca
     2581 aaatttcgga cttggattcg gtcttatacg tataacaaag gtatgtatgt cagcaatgtc
     2641 aaggatgagc aagcagatga aaaaggcgta tatgaaatgt attatgactt tgcagaaccc
     2701 gtccataaaa tggtttctca tcgaatatta gctactaatc gtggagaaaa agaagatatt
     2761 ctgaaagtat tctttcaagt agatgaagca gcaattttag cttacttgga tcgccaaata
     2821 gtcaaaaatc ctgcctctcc ttcttcttca ttcgtcagag aagcctatca ggatagttat
     2881 aaacgcttta ttcagccagc aatcgagcga gaactgcgca atgaattgac tgaaaaggca
     2941 gatgagcaag caatcgctat tttcggcgaa aacctccgta atcttttgtt gcaaccgcca
     3001 ctaaaaggta aagttgtcct gggatttgat ccggcttatc gtactggatg taaactagca
     3061 gtcgtagatg cgacagggaa agttttagca atcgaagtta tttatcctca taaacctgca
     3121 gcgcaagcaa aaagagaagc tgctggacca gcttttatcc aattaattaa taaatatcaa
     3181 gtagatatgg tagctatcgg aaatggtaca gccagtcggg aatctgaact ttttgtggct
     3241 gagcagttga aatccgccga ccataaaacc tattacgcta tcgtcaacga agcaggcgct
     3301 tctgtttact ccgctagcga aattgcacga aaagaatttc cgcatctaca agttgaagaa
     3361 agaagtgctg tttctattgc ccgtcgcctg caagatcctt tagctgaatt agtcaaaatc
     3421 gatcctaaag ctgtcggagt aggccagtac caacatgacg tttctcaaaa acgtttggct
     3481 gaacaattgg attttgttgt ggaaacagcg gttaaccaag taggtgtgga tgtgaataca
     3541 gcaagcccgc agctcttgca gcatatatca ggattgaata aaacaactgc ccaaaacatt
     3601 gtcatttatc gtgaagaaaa cggagaattc accgcacgaa ctcaattgaa aaaagtgcca
     3661 cgcttaggac caaaagcata tgaacaagca atcggattct tgcgagtccc tggtgggaaa
     3721 aacattttgg acaacacagg tatccatccc gaaagttatt ctattgcaaa agaaatatta
     3781 acttcagtcc aactttcaga aaaagagctt ggtacagaag aagcagtaga aaaattgaca
     3841 cgattatccg tagagaaact ggctgaaagc ctatcagtcg gagaagaaac gttagccgat
     3901 attttagctg ggctgacaca acctggacga gatatgcgtg atgagatgcc agcgccctta
     3961 ttacgtacag atgtattgag tatggaagac ttgaaaccag ggatggaatt gactggaacg
     4021 gtccgtaatg tcattgactt cggcgcattt gttgatatcg gtgtgaagca agacggactt
     4081 gtccatatat cgaaactcag caaaaaattc gtcaagcatc ctacagatgt cgtgtcagta
     4141 ggagatatcg taacagtttg gatagaacaa gtcgatacga aaaaaggcag aatcagtttg
     4201 acgatgcttt ccccttatga agagtaacaa tgaggagtaa caaaaattga cacaagaaga
     4261 actgcagcag ttagtagaag agatatcatt aaaaagcttc cagcttcctt tcaagcacca
     4321 agcaatgttc aatccacgct tacgtacaac tggcgggcgc tatcatttaa acgatcatca
     4381 tttagatttt aatgcgaagc tatttgaaga gtcagacgaa gagacgattg caggaatcat
     4441 caaacatgaa ctttgccatt atcatcttca tctagctgga agagggtatc agcataaaga
     4501 tatggatttc aagtatcttt tgaaaaaaac aggagggttg cgctatgcac cagccttgac
     4561 gaagaaacag ataaagatgg agtattatcg atgccaagat tgttctgcca cgatctgccg
     4621 caagagaaga atcgatgtac gcaaatatcg ttgtggaaga tgtcatggag aaatcaaatg
     4681 gattgagaca aaaagagaag actttaatga agcacaaaaa atcagatgaa aggatagaag
     4741 gcctttggaa aaaagtgagc agaggaatct aaaagactgg aatcagctat taaatttttt
     4801 agccgcacct gaagagggaa attttgagga actgacggca cctacactta ttttagctgg
     4861 aaactgtctt cctatcttag ctgataaagc agcacaaatg tatttaaatg gccaagtaga
     4921 tcaattgttt ttggtcggag gggtggggca tgcgactaga atcctttatg agaattttga
     4981 aaagcaagga tttcattttg aagatggcat gagtgaaagt gaaatctgtc gtcagtatct
     5041 aaaagaagtc tatgatttgc ccgataaggc ttttctgata gaatcgaagt ctacaaattc
     5101 tggagagaac gccacttttt cactggagat tcttcattcc ttaggagctg taccagaaaa
     5161 aatcttattg atgaatgatc cgacactgca acgcaggact cgagcaactt ttgagaaagt
     5221 atggcaaaat gaacaaacta ttttcgcgaa tgctgtgcca tttgtgccgg aaatccttca
     5281 cttttctgaa gagatcatct ttacagcaaa agaactgaat catcaatggc ctaaggaata
     5341 tttccatgcg ctagtattag gagaaatgga acgtctccat gacgatgaga acggctatgg
     5401 tccgaaagga aaagatttta ttcctcacat tgatatatca gaggaagttt ggtcttctta
     5461 caccagaatt aaagagtcaa taaaaacgga tttttctcgt acttgaccat tttagcaagc
     5521 gaatcctcct ttccaaataa atttggttga aattttcaat cgtttataag ataatgagga
     5581 gcgtgttgaa tgtaatagct aaaagaatgg acgctagata agagaagagg attcaaatgg
     5641 aaaatcaaaa taaattgtat cattttgttg gtatcaaagg ttcaggaatg agctccttgg
     5701 ctcttgtttt acatgaacaa ggtctaaatg tccaaggatc agacattgaa aaatatttct
     5761 ttacacaaag agatttagaa aaagcaaata tcacgattct gccatttaac gcagataatg
     5821 taaaaccagg gatgacgatc attgcaggaa atgcatttcc tgattcacat gaagaaatcc
     5881 agcgagcaaa agaattaggt ttggaagtga ttcgctatca cgactttatt ggtcatttta
     5941 tccaaaatta tacgagtatc gctgtaacag gctctcacgg aaaaacaagt acgactggac
     6001 tgctctctca tgtattgagt ggtgtccgtc caacaagtta tctaattgga gatggaacag
     6061 gacatggtga tccgcaagcg gaattctttt catttgaggc ctgtgaatat cgccgtcatt
     6121 tccttgccta ttcaccagac tatgcaatca tgaccaatat cgattttgat catccggatt
     6181 attatacaag tatcgacgat gtatttacag ctttccaaac aatggctgga caagtgaaaa
     6241 aagctatttt tgcgtatggt gacgatgctt atttacgcaa attaaaagca aatgtgccga
     6301 tttattatta tggtgtcaca gaaaacgatg atatccaagc acgaaacatc gaacgcacaa
     6361 cttctggttc tgcttttgat gtctatcatg gggatgaatt tgttgggcac ttcactgtac
     6421 cagcattcgg taaacacaat atcttaaatg ctttaggtgt tattgcagta gcttatttcg
     6481 aaaaactgga tctgaaagaa gttgcagaag aaatgctgac tttcccagga gtaaaacgtc
     6541 gattcagtga aaagatcgta gcagacatga ccgtagtcga tgattatgcg catcaccctg
     6601 cagagatcaa agcaacgatt gatggcgcac gccaaaaata tccagataaa gaaataattg
     6661 cagttttcca accccatacg tttacgcgta cgattgcatt gatggatgaa tttgcagaag
     6721 cacttgattt agctgataaa gtttacttat gcgatatttt tggttcggct cgggaagaac
     6781 aaggaaacgt taagattgaa gaccttggag cgaaaatcaa aaaaggtgga gaagtcatca
     6841 aagaaaataa tgtatctcca ttgttagatt atcatgatgc agtagtaatc ttcatgggtg
     6901 ctggagatgt tcaaaaattt gaacaagcgt atgaaaaact tttaagcagc acgacgaaaa
     6961 atgtactgta atacaattac tgcagtgtcc ttataaaaat agaaagaggg ggagtggcaa
     7021 aaatcttgcc aagccccctt ttttgcaact tatgataaac ggtgttcaaa agcttgcatc
     7081 tgcggtaata agcccaatca aatcaaaaac atgagaagct gttttttctt ggctgttctt
     7141 attatttgat gcaggacagc tttttcacga cctctgtaaa ctataggaat ctgttaaatc
     7201 aagggagcag aacttgcctt ttacgctact tttggtacaa taatgtatga ttaaaaataa
     7261 actggagggt ttctatcttg aacataggta agccgagaaa actaggaaaa acaataaatg
     7321 aaatcgagga aggcgactcg ttatccttaa cagaatctat tgaagataac cagcttttgc
     7381 tgtatttggg attgacgaat gatgcaaatc ctttgtatat ccaacatgac tttgcacaaa
     7441 aaacagaata tcaacagcca atcgttccat cagtgatgct gatggggatc atcacaagtg
     7501 caatttcaaa gcatcttcct ggaccaggat cacatgttgt caatttttct gtcaatttta
     7561 ttgaaccggt tttccattat gaaacactga cttttcagtt tgaggtcata aaagtagata
     7621 agatgaagga tgtggtgacg atttctgtag aagcggagaa tacgaacgaa gatcgtgtgt
     7681 tggatgctgt cgttatggtc caaccgccac aagttcctga attggaaata gcaatcgaaa
     7741 gtgaaggtga attgaatgaa taatccaagt acatcaatgt caggtatcaa tcgtttttat
     7801 gcaaaagttt atggattttt aggtatggga cttgccctta gtgcagtcac ttcttacttg
     7861 gctctttggg tatttccttt gcaattagca acttttatca ataattttcc actaggcttt
     7921 gtgggtctgt ggatcgttga actgatttta gtaatggttc taggcgtaaa agcacaaaag
     7981 aatccgagct tagcaatcgg tggatttatt gcctactctc ttttgaatgg cataacatta
     8041 gcggttactc ttgcatacta cgatattgga acagtaacgc aagctttctt gacagctact
     8101 ttgatgttca tggggatgtc tgttgtcgga gtaacaacaa aaagagatct tagcggggtt
     8161 ggaagagcag gacaggcagc tttaatcgga gtgatcattg cactagtctt gaacatcttc
     8221 attttgagaa gtagcgtagt tgagctgttc atctccctac tgttagtcgt catctttgca
     8281 gggattacag cttatgataa ccaaagcatc cgtaatatct atgtccagac aaacggaaca
     8341 gcacatagtg gaattgctgt attcatggca ttgaagctgt atttggactt tatcaacttg
     8401 ttcttggcgt ttctgagaat ctttggacgg aacaactaaa aaagaaaaaa attcatcagt
     8461 tggggctata ccgaattatt tggtatggtc ccgctttttc ttttcgcatt ccgcaggatt
     8521 ctttgttaga atgaataaga actttacagt aaggagttaa gaagatgaca aaaaataaat
     8581 tattactcgt tgatgggaac agtgtggctt ttcgcgcatt ctttgcgctt cacaattctc
     8641 ttgaacgttt caaaaataga aacggtcttc atacaaatgc aatctatgca ttcaatacga
     8701 tgtttgaaaa tgttatgcaa aaagagcaac cgactcatgt tctggttgcg ttcgatgcag
     8761 gaaaaacgac ttttcggaca gaaatgtatg cggagtacaa aggtggaaga tccaaaacac
     8821 ctggcgaatt caaagaacaa atgccatata tacgtgattt gttgacagga ctaggggtac
     8881 agtattacga attgcccaat tatgaagcag atgatattat cggtactttg gcagaaaaag
     8941 tagacaaaga tcaatttgat gtcgtcgtgc tttcaggtga tcgggatttg actcaattag
     9001 ctagtaaaga agtaaaagtg gatatcactg taaaaggtgt cagcgatatc gaaagctata
     9061 caccagaaca tgtagcggaa aaatatgatg ggttgacgcc aaaacagatt attgacatga
     9121 agggactagc cggagatgcc tctgacaata tccccggtgt gactaaaatc ggtgaaaaaa
     9181 cagctatcaa gctattgaaa caatatggct cagtcgaagg aatttatgaa catatcgatg
     9241 aaatgaagca aagcaaaatg aaagaaaatc tgataaatga caaagagcag gctttcttat
     9301 cgaaaaaatt ggcgaccatc aatacttcag cgcctgtgga tgtctctatc gactcattga
     9361 agtatgaagg gaaaaatctc gataaactcg ttccgtttta taaagaaatg gatttcaatc
     9421 agttcctttc taaattgaat atcgtagaag aagaagtaaa aatggatgat attttgtttg
     9481 aagtcgttca tgaattcaaa gaggaaatgt ttactacgga tatggcgttg tacgtggaaa
     9541 tgatgggcga caattatcat acagaggaaa tcgtcggagt agcgtggggg acagaaaaga
     9601 aaatttatgt cacgaacgat ctatctgtat tcgaaaaccg agcgttccat tcttggatca
     9661 ctgatccgac tcggctgaaa aaagtatatg atgcgaaacg aacttatgtt gctttaaacc
     9721 gctatactgg aaaaacgaaa ggaatcgatt ttgatgtctt gctaggtgca taccttctcg
     9781 atacgaatga aaaaagcaca gatatcgaag gaatagctgc tcattacgga tacaacgata
     9841 tccaatcaga tgaagctgta tacggcaaag gggcgaaaaa aggtttacca gaagaagagg
     9901 aactcttttt tgctcattta gcgcgaaaag tagctgcaat caatgcgttg acaccaaaat
     9961 tgagccaaga actggcagat aaaaaccaag aagatctttt ccggaaaatg gaactgccat
    10021 tgtctatcat tttggcagag atggaaataa gcgggatcaa agtagatgcg actcgcttac
    10081 aagaaatgaa gggcgagttc tctgctcgct taagagagat cgaacaaaaa atctacgaag
    10141 aagccggcga agaattcaat ttgaattcac caaagcaatt aggagtgatc ttatttgaaa
    10201 aaatgggatt acccgttatc aaaaaaacaa aaactggata ttccacagcc gtagatgtac
    10261 tggaacagct tcgtgaacaa gcaccaatcg tagaggatat cctcacctat cgtcaaattg
    10321 caaaaattca atctacctat gtcgagggac tgctgaaagt gatccaatca gatggaaaag
    10381 tgcatacccg ttatgtccag acattgaccc aaacaggacg attaagctct gttgatccta
    10441 atttgcagaa catcccgatt cgattagatg aagggagaaa gatacgtcag gcatttgttc
    10501 ctagagaaaa agactggctg atttattctt ctgactattc acaaatcgag ttacgcgtac
    10561 ttgcgcatat ctcaaatgat gaacatttga aagctgcctt cctagaaggt caagatatcc
    10621 atgccagcac agcgatgcgt gtatttggaa tcgaaaaggc agaggatgtc acaccaaata
    10681 tgcgtaggca ggcaaaagca gtgaattttg gtatcgtgta tggcatctct gattacggac
    10741 tttcacaaaa tctaggaatc agccgaaaag cagcacagca atacatcgat acgtactttg
    10801 aaaaataccc aggtgtcaaa gaatatatgg aaagaatcgt acgcgaagca aaagaccaag
    10861 gctacgtcga aacgctgtat caccgccgtc gatatctgcc agacatcaat tcaagaaatt
    10921 acaatatccg ttcttttgcg gaacgaacag ctatcaatac accaatccaa ggaagtgctg
    10981 ctgatatttt gaaaatcgcg atgatcgaat tggataaacg attaaaagaa acaggtcttc
    11041 aagctacgat gctgttacaa gttcatgatg aacttgtctt cgaggtaccg gaaaaagaac
    11101 ttgaatcatt agataaattg gtgaaggaag ttatggaaca agctgtttct cttcatgtac
    11161 cactgatcac tgacagcagt tggggaaaaa cgtggtacga agcaaaataa acattgttta
    11221 agacagagca atataatgaa ggtgaaaaga aaaaatgcca gaattaccag aagtagaaac
    11281 cgttcgtaaa ggattggaac ggttagtagt aggaaaaacg attcaaaaag ttcaggtctt
    11341 gtggcccaag atcatcgagc aaccagagac acccatcttt gaggcttctc ttgttggcga
    11401 aaccatccaa tccattggac gtagagggaa gtttttaatt tttcatctcg atcattgtga
    11461 actgatctca cacttgcgaa tggaaggaaa atatcaattt acgaaagaaa atacaccaat
    11521 cgataaacat acgcatgtac ttttcttttt tgaggatgga agccaacttc gctacaacga
    11581 tgtacgaaaa tttggtcgta tgacaatcgt cgaaaaagga gcaagtgcaa cttaccgagg
    11641 aatcatgaaa ttgggacctg agcctttgcc agatagcttc ttattagcag atttcgcaaa
    11701 tggactgaaa aagtcccaca aagcaatcaa gccattgctg ctagaccaac gtctagtgac
    11761 tggtctaggt aatatttatg tagatgaagc attatgggaa gcgaagatcc atccagaaca
    11821 accggcgaac acactgcgcc ccaaagaggt agaacaatta cgtctatcga tcattgatgt
    11881 tttggatcgt gcaatagaag ccggaggtac cacgattcgc agttatttga atgcattagg
    11941 agaatcaggc ggatttcaag tagcactgca tgtctatcag caaactggca aaccatgtat
    12001 acgttgtgga acgccgatcg tgaaaacaaa agtggcacaa agggggacgc actattgtcc
    12061 gaattgtcaa aggctaaaaa tcgcaaagta ggatttgttt taggtttgac gggtggaata
    12121 gctaccggta aaagtacggc agccaaagtc tttcagtctc atggatttcc tttgatcgat
    12181 ggcgatgtga ttgccagaga aactgttgag cctggaacgc ttgctctgag gaaaattgta
    12241 tcagtgtttg gccaagaggt attgcagcct gatgggcaac tagatcgagg gaaacttggg
    12301 atgattgttt ttccatcaaa ggaaaaacgg caaaaacttg atcagctatt agatccgttt
    12361 atccgaaaag cgataaaaga tcaaatcgcc tctctaagtt ccaatcatcc tttagtaatt
    12421 gtagatatcc cattgctcta cgaaggtcat tatgaccact atatggatgc agtagctgtc
    12481 gtttatacta cgccagaaac tcagcttcag cgtttgatga aacgtaatca actgacgctg
    12541 gaacaagcgc agcagagaat tagtagtcaa cttccaatcg aagaaaaaaa gcagcgtgcg
    12601 gatattttgt tcgacaacaa tggaacaaaa gaaaaacttg tgggacaaat cgaaaattgg
    12661 ctttttgcta atcgctttct ttgagtatct gtgtcaattt tgctatcctg agtatataga
    12721 aagtgaggga tagcgatggg atcattaaaa aatcaaaagg ataaagtcgt aggtaacgca
    12781 aaagaaaaaa tcggcgagat gctcaataaa gaaaatctaa tcgatgaagg tcgtacgcaa
    12841 tcagaaacag cagaagaaaa agaaaagcag taccaagcaa gaatcgatga aatagaagac
    12901 cagcaggcag aagaagaagc gaagcggcaa gatttgactc aagaacgaaa aaatccgaca
    12961 cctgatcgta gtaaaacacc gctcccagaa agtgaccaca tttccgatga gcaaaaaatc
    13021 gaggaacagc gaatgaaaca ctatacagga atcgaagaga agttataaaa tagtaaaaaa
    13081 tcgagatgtg acttgaagca gatgagaagg tgttcatctg cttcaag
//
LOCUS       DACECZ010000076        12971 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.076, whole genome shotgun sequence.
ACCESSION   DACECZ010000076 DACECZ010000000
VERSION     DACECZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.076"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(187..1635)
                     /gene="cls"
                     /locus_tag="IWO35_001911"
     CDS             complement(187..1635)
                     /gene="cls"
                     /locus_tag="IWO35_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0316968.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1787..2680)
                     /locus_tag="IWO35_001912"
     CDS             complement(1787..2680)
                     /locus_tag="IWO35_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0316969.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2664..3350)
                     /gene="ftsE"
                     /locus_tag="IWO35_001913"
     CDS             complement(2664..3350)
                     /gene="ftsE"
                     /locus_tag="IWO35_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ0316970.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3455..4556)
                     /gene="prfB"
                     /locus_tag="IWO35_001914"
     CDS             complement(join(3455..4492,4494..4556))
                     /gene="prfB"
                     /locus_tag="IWO35_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ0316971.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4682..7216)
                     /gene="secA"
                     /locus_tag="IWO35_001915"
     CDS             complement(4682..7216)
                     /gene="secA"
                     /locus_tag="IWO35_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ0316972.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(7438..7989)
                     /gene="raiA"
                     /locus_tag="IWO35_001916"
     CDS             complement(7438..7989)
                     /gene="raiA"
                     /locus_tag="IWO35_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ0316973.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(8117..8836)
                     /locus_tag="IWO35_001917"
     CDS             complement(8117..8836)
                     /locus_tag="IWO35_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ0316974.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYRWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(8833..10146)
                     /locus_tag="IWO35_001918"
     CDS             complement(8833..10146)
                     /locus_tag="IWO35_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0316975.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKVCQEIKEMNEEGKKML"
     gene            complement(10227..10547)
                     /locus_tag="IWO35_001919"
     CDS             complement(10227..10547)
                     /locus_tag="IWO35_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0316976.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(10655..10942)
                     /locus_tag="IWO35_001920"
     CDS             complement(10655..10942)
                     /locus_tag="IWO35_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0316977.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            11069..11713
                     /locus_tag="IWO35_001921"
     CDS             11069..11713
                     /locus_tag="IWO35_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ0316978.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(11772..12479)
                     /locus_tag="IWO35_001922"
     CDS             complement(11772..12479)
                     /locus_tag="IWO35_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0316979.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
BASE COUNT         3588 a         2813 c         2251 g         4319 t
ORIGIN      
        1 atgtgtctct tttaatagca tatattagct tatttcttgc tttccccaaa aaacaagaga
       61 taccccaaag ttaatttcgc tcatgcgaga cgctttctcc atttctgacg tactctcaga
      121 aatggccccg ttagaaaagg acacgagatc tccatctgtt gatggttctc atgtcctttt
      181 tatgttttaa aggagcggcg aaatcaatct tgctaatcct tctttcatct tgatgattat
      241 tcctcggttt tcatacattt cttttgtgag atgcgtagaa attttcgagt cttcaaaaaa
      301 agcttttctt acacgtgaag ccattcgttc atcataaacc agtgtattga cctcaaagtc
      361 caagcgaaat gaacgaacat cgatatttgc tgagccaact gaaacaatgc caccatcgat
      421 aatcatcgtc tttgcatgaa taaatccatt ctcatatgtt tcgatccttg ctccatactc
      481 gatcagctct gcagcgaaag aatacgttgc ccagtaaacg agaagatgat ctggtttgtt
      541 aggtatttgg atatgaactt ttacgcctga aagaagagcc aacttcagtg cttcatgaat
      601 cgatccatcc ggtatgtaat aaggcgtttg gatcaatatc tcccgtttgg ctaacgaaat
      661 cattttcaaa taagtcattt tgatttgttc atgttcggta tctggtccgc ttgtcacgat
      721 ttgaacagct atatcaccag tagtgtgaat ggacggaaaa aatttgggag aatagcctac
      781 ttcttttgta tgctgagaat tccagtccat caaaaaacgg ttttgcaagg tatacaccgc
      841 ttctccgtaa atacgcaaat gattgtctcg ccaataacca aatttcttta ccaatcctag
      901 atattcattt ccaacattga acccacctgt atatccgatt ttcccatcaa taactacgat
      961 tttacgatga ttgcgataat tgatccgagg attaatatac ggaacaaata gaggaaagaa
     1021 aaatgcgact tctccgcctg cttttttcaa attctgaaaa aagctggaag aaacttgtgt
     1081 ggaaccccat gcatcaagaa ggactctgac ttttacccct cgtcttgccg cctctgtcaa
     1141 tgcatctcga acttcttcac ctaatgcatc actgcgataa atataatact ggaagtggat
     1201 atggtcttct gcctgcttga tatcttcaat cagcgcatcg aatttttcac gaccatctgt
     1261 aaataaagtg atttcatttt tagtcgtata gagcgtactt tcaaataccg tgagcatata
     1321 gatcaattgt ttcacatcta cttgtccagt aggcggatga ggataaagcc ctcgcatcaa
     1381 tgcctgtttg tcttcttcaa tttccaggtt cttaccgatt ttcccttgga tcttcaaatc
     1441 aaagattttc tctttggaaa tccctcttcc taaaaaaata tataaaataa atcctacaac
     1501 tggaatgaac atcaaaacga gcagccatgc ccatgtttga gcagtttcct ttctttctcg
     1561 aaaaacgatg ataaaggaaa gcaaaatatt cataatcaaa aggatcgtaa aaaaattgtc
     1621 aacgataatt ttcacggctt cacctcttct tctaccaact taagaatacc caagttgttt
     1681 tttaaagtaa agggaaaaaa gccgcttcct tctattctcc tagccaataa aaaaagaaag
     1741 ctggaacgaa actgtccaac tttctcattt tatttatatt gtggtattag aatttcaaga
     1801 aacgtctcat agaaatgatc gagccaagtg aaccaatgat cacaccaatg ccgaccatta
     1861 aaagattgat tttccaaacg atattttgtg gatgaatcaa tgaataatgg gaacgtaata
     1921 atgatggatt gatcaattgg aaaaaccatg cataaccaaa tgtgatgatc aaaatcggaa
     1981 gaatcgcacc gatcagaccg atccagccac cttcaaggaa aaacggccag cgaatatatc
     2041 cattttttgc accgactaaa cgcatgattt gaatttcacg ctgtctagac aaaatcgtga
     2101 tccgaatcgt gttcgaaatg agaaacatcg ctacgaacag cagcaatgcc gctgcaacta
     2161 atccccatgt ccgaactgca ccagcgattt ggaaaatccg atctgaggaa gctccaccgt
     2221 aatcagaacg agaaacattc ttcaaatctg ctgcttcttc tgctacttgt tttgtataag
     2281 aaggatcagt agcacttact acatacacat catataacgg gttattgtca ccttcaaaca
     2341 atttccagga atcacccatt tcgttttgga tttttttcaa ttcctgttct ttgcttgaaa
     2401 attcaacttt tttaacatga tctaagtcat tcaattcttt ttctagcttt ttcatctctt
     2461 cgtcttttgt accgatatct acgaatacag atacatcaac gtttccttct atatcctgag
     2521 ctaatttagt tgcattcatg ataactgcca tgaatacacc gactaaagtc aacgtgatcg
     2581 ttaccgcact gactgaagca actgtcatcc agccgttacg tttcaaactc ttgatacttt
     2641 ctaataaatg gcggaaaaag gttctaatca tcgtatccat attccccttc cgcttgatct
     2701 cgaataatgc ggccgttttc gatagcaatt actcggtgcc gaatcgtatt tacgatcgta
     2761 ctgttatgtg tagccatcac gacagtagtt ccttgggcat tgatccgatc taataatttc
     2821 atgatttccc atgagttttc tggatccaga tttcctgtcg gttcatccgc gataagtact
     2881 ttaggcgtat tcacgattgc ccgagcaata gagacacgtt gctgttcccc accggataat
     2941 tcgctaggaa agactcgaac tttgtgtttc aaaccaacaa ggtccagcac ttccattact
     3001 cgttttttga tatctcgagg ttttctgccg ataacttgca tagcgtacgc aacattttca
     3061 tatactgttt ttcttggtaa taatttatag tcttggaaca ccacgccgat ttctctgcgt
     3121 agataaggaa cttcgcggtt tttgattgct attagatcat gacctgcgac gttcaaagtt
     3181 ccttttgaag ctttttcttc tcgatacatc aatttgatga atgtagattt accggctcca
     3241 gacggaccaa ccacatagac aaactctcct tgttcgatga caatcgagag attgcggata
     3301 gcggtcgtac catttgagta cttcttcatt acatccttca tttcaatcat ggcgttctct
     3361 ccatatcttt ttcagtcttt agctattata gcatggcaat gtttcaacat gcttgcaaaa
     3421 tcttacaatt ttatttcaat ttaacaatgg gttcttattg tagatgcatt tttaaatagc
     3481 cgtcaataaa taaatcaagg tcaccgtcca taactgcttg gacatttcct gtctctacgt
     3541 ttgtccgatg gtcttttacc atagaatagg gatggaaaac ataagaacgg atctgtgaac
     3601 cccagccgat ttctttttgt tctcctcgaa gtgcagctgc ttcttgttct ttcttttcca
     3661 cttctaattg ataaagctta gcttttaaca tccccatcgc ttgctcacgg tttttcaatt
     3721 gtgagcgttg tgcctgacta gctactactg ttcctgtagg caagtgagtg atccgtacag
     3781 cagattctgt tttattgatg tgctgaccac ctgcaccact tgctcggtac gtatcgattt
     3841 tcaaatcatc tggattgatt tctatatcga tcgtttcatc aagctctggc atcacatcta
     3901 ccgaacaaaa agaagtatga cgtcgtttcg ctgagtcaaa aggcgaaatc cgtacaaggc
     3961 gatgaacacc tttttctgat tttagataac caaaagcgtt atgtccttta atcagcaggg
     4021 tcacactctt gatccctgct tcatcacctg cttgataatc tagtgtttct acttgatagc
     4081 catgctgctc tgcccaacgt gtatacatac gtaaaagcat gctgccccag tcttgggact
     4141 ccgttccgcc agcccctgga tgcaattcga cgatcgcatt gtttcgatca taaggtccgt
     4201 ctaacagcat agataattca tacgtcttca ttttttcatc taatgcattg atacgtgttt
     4261 ctgcttcttc ttgcattgct gcatcaggtt cttcttgcag catctccacc atcaattcga
     4321 tttcttctag ttcttcggca agagaatgga attggtcata ggtttcttta ttagcgttat
     4381 tttcgctgat cactttttgt gcggcttcgc tgtcatccca aaaaccaggt tcagccattt
     4441 ttgcttctgc ttccgcgatg tcttcttcca gctgatctaa gtcaaagaga cctcctgaaa
     4501 cttgtgacct tttcctgcat ttctgtcagg agatttcgaa tttcggatat ttccatgttt
     4561 tttccttctt tcactatact tttttcatta tatctagtct aacagaatca gcaaataaca
     4621 gaaagaaggt gagacgaatt cttaaaaaaa gaatttgtct caccaatcct gctgtcagaa
     4681 attagtttgc attttttcca tgacagttct tatatttttt cccgcttcca caaggacaag
     4741 gatcatttct tccgacctta ttcacatgga ttggctgttt tttcgcgcta gtattactat
     4801 tttctttggc atctccgtct tctgagtgag aagctgttcc ttgagaaacc tgttcacgct
     4861 gtacgttttg acggatctca gctttcataa agattcgagt gacttcatac tcgatggcgc
     4921 ctaccatatc ttcaaacatc ttatagcctt ctgtttgata ttcgactaat ggattgttct
     4981 gtccataagc acgtaagccg attgactgac gtaattgatc cattgcatca atatggtctg
     5041 tccatttaga gtccacaaca cgcaagatca cgactttttc aaattccaac agttgctctg
     5101 gaccatttag ttgagccgct ttggtattaa atacttcttg tgctcgttcg tttagatact
     5161 ctttgatctc ctgagctgtt tttccttcga tatctgcaag agtaatgcta tcttcatgga
     5221 caagagtact gccggcaaaa tcgatgatac tttccaaatt ccaattttct ttctctaatt
     5281 gtgtatgcgc atctactaca cgactgattg tccgtttgac catgttcatt agtggcttgg
     5341 ataagctttt ttcttccatg attacttctt gtcgctgttt atagattact tcacgttgtt
     5401 cacgcatcac atcatcgtat tgtaagacgt ttttacgtgt gtcatagtta tttccttcta
     5461 cacgtttttg tgcagattct acttgtttcg tcagcatctt gctttggata acagcatctt
     5521 cctcaccgat cttcatccga tctaagaaag ctttgattcg ttctgaaccg aatcgtttca
     5581 tcaaatcatc ttccaatgac aagtagaact gtgacatacc agggtctcct tgacgtcctg
     5641 cacgtcctcg aagctggtta tcgatacgac gagattcatg tcgttctgtc ccgataactg
     5701 ctagaccacc aagttctctt accccaagtc ctaacttgat atctgttccc cgaccagcca
     5761 tgttggtagc aatcgtcaca gcaccttttt gaccagcatt catgatgatc tctgcttctt
     5821 taaagtgatt tttcgcattc agcacttcat gaggaatacg ttcttgattc agcatatctg
     5881 ataataactc agatgtttca accgcaacag ttccgactaa cacaggttga cctttgcggt
     5941 agcgatcttt gatatcttgc acgacagcgt ggaacttgct ttgtaatgtt gggtaaagca
     6001 aatctggacg atcatcacgg ataaccggac gatttgtcgg aatttggtat acttggatgt
     6061 tataaatttc tctgaattct tcttcttctg ttttagctgt accagtcatt cccgcaagtt
     6121 tcttgtacat acggaaatag ttctggaaag tgatcgtcgc catggttttc gtttcgtctt
     6181 cgatctctac gccttcttta gcttcgattg cttggtgaag tccatctgaa taacggcgtc
     6241 catccatgat ccgaccagtg aactggtcaa caatcagtac ttttccgtct tggacaacat
     6301 aatcgatatc taaaagcatg atatagttgg ctctcaatgc ttgatccaag tgatgggtca
     6361 atgcagtatt ttcaatgtcg tataagttat ccaaaccgaa tgtttcttct gctttttcga
     6421 tccctgcttc ggttaaactg attgtttttg attgtacatc gattttataa tcttcttctt
     6481 ctttcaaacg tttcacgaag ttgtctgctc ttgtatataa agctgtggat ttttctgctt
     6541 gccctgaaat gatcagcggc gttctcgctt catcaatcaa gattgagtca acttcatcga
     6601 caatggcata attcagcgga cgttgtacca tttgatggcg gtaaacgacc atattgtctc
     6661 tcaagtagtc aaatcctaat tcattattcg tactataagt gatatcacaa ttataagcct
     6721 cgcgtttttc atccgaagat tttgagttga tattcaaccc cactgttaat cctaagaaat
     6781 tataaagttc gcccatttcc gtcgagtcac gggttgctaa atattcattg acggtaacta
     6841 cgtgtacccc ttcgccagtc agagcattta gatatactgg catcgttgcg gtcaaggttt
     6901 taccttcacc agtacgcatt tcagggatat ttccatcgtg aagcacgatt ccacccatca
     6961 attgtacgtg gtatggatat aaacctaata cacgttttgc tgcttctcgg acgactgcaa
     7021 aagcttccgg aagtaattga tctaatgttt ctcctttttg gtatctgccc ttgaattcag
     7081 cagtttttgc ctgtaattgt tcatcagtta atgctgccat ttggtcagcg tatgcttcaa
     7141 ctttttttgc aatgctatct aaacgtttta gttctttctt atcgttttcg ataagtgaac
     7201 gaatcaaatt ggccatgcgt tgtgtaatct ccttttcctt ctgtgtatta tccagactat
     7261 gtagacttta cttttcattt tatcattagt tatagataat tgaaatatct taagcgtatt
     7321 aaagtaagaa cttcataatt tattcatatt tcatctaaaa gaggaggaaa ctcaaaaaaa
     7381 cttgcggcag ctgtcagtag ctgctgcaag ttttcgttgt tatttaagta aggctgatca
     7441 gtctgtttca atcagtccgt attttccatc tttacggcgg taaacgatac ttgtaccatt
     7501 tgtttctgca tcttcaaaaa taaagaagtt atgtcccagc atgttcattt gtaagactgc
     7561 ttcttcacta tccattggtt tcaatgaaag gcgtttcgta cgtacgatat ctaattcaga
     7621 atcattttcg ttttcgtcct cttcattgaa taaagcagca gcatctgcag tattcatgcc
     7681 cgtttcacgg gatttacgat taatttttgt tttgaatttg cggatttggc gttccaattt
     7741 atcaacaacc aaatcgatac ttgcgtataa atctggagaa gtttcttcgg cacgtaatac
     7801 aagataaggt aatgggatcg ttacttccac ttttgctgtt ttttctgtat aaacttttaa
     7861 gttcacatgc gccgttgcat cgggtgcgtc actgaaatag cgttctagtt tgcctacttt
     7921 tttctcaacg tagtcgcgaa tcgcttcagt tacttcgata ttttcgccgc gtacattata
     7981 tctaaacata aaaattaccc ctttcgtcta ccacaatcaa gaaggagaag gaccactctt
     8041 tccctcttct taattctatt atatcgaact ctaggttatt tgcaaattaa atcgctatca
     8101 aatttccaca tctaattcat ctcgccaaag aaatggttcg gatgattttc ggctgtgcga
     8161 cctgcaaaca atctgctgcg tggaaaagag tctgtcctgt tgtatagaca tcatcaataa
     8221 tcaatatttt tttattttgt atcttagaaa aggtgactgc aagttcaaaa ggttgaggca
     8281 tctccaatct ttcctttcgc gtgttttttg cttgagccag cagatgcttc tctcttttga
     8341 gtagcatctg aaaaggaatt cttgctgctt tcaacatctc gcttacttga ttgaatcctc
     8401 tttctgttcg ccttttttca gataaaggta ttggacaata gatgtatgtc gggtattctt
     8461 tttttatctg cgtccatcta tatgcaaagg tgcctgctag tcggatatct cccataaact
     8521 tgtattgatt gagccaggcc ttcatatttt catcgtacac aaaaaaggca cggtgctgaa
     8581 aatcataggt cggatatttc ttcttccatt cttgacagtc actgcagtaa acgggaatag
     8641 actttacttt ctttttcgta ctctctagat tgaaatggat tggtttttga cagccagaac
     8701 agcctcttgt ttccaataaa tgaaattgtg cgcgacaaac tgaacaagtc tgaggtaatc
     8761 tgtatttcga aacaccaata atctcgctga atgttaaatt ttggaagata tctttactgc
     8821 agcaactgca cctcacagca tcttcttccc ttcttcattc atttctttga tttcctgaca
     8881 aacttttcga atagctgcag ttttttcttc atagaaaaag tatacttgac tgttcgtata
     8941 aggcccttta cgatcggctc ttcctgcgat ttgtaccaaa gaagatttag taaaaaccgg
     9001 atgatccgct cctaaaacga taacggatat tctttcaaat gtgactccac gttcaaggat
     9061 catcgtggtt aacagtatgt catattttgc ttctcgcatt ttctggactt tttccgcccg
     9121 ctgattgtct cttgaagaaa catctgtgat ctcatatctc ggaagagcct cttgaagtgt
     9181 ggctttcaaa cgatgcatca tcgtgatact tggacaaaat aaaaggatat ggttttcttt
     9241 aattaattga tgaatgatcc gcaccaaaga ttttatcttc ttcttgttct tgcatttttg
     9301 tcgccaatta ttcatgaaaa ccaagacggg ttcaggaagc aaacgccgat gaaagcgtaa
     9361 agcaagtttg tggatgtcaa acgtctgctt gatttccttt agtaactttt tatcaggtgt
     9421 ggcacttaag tagatgaatc gtccgtctgg tttaatcgct gtttgcacag catgagctaa
     9481 tcctgcatcc ccgttataag gaaacgcatc gatctcatcg atcactatta aatcaaagga
     9541 ccgataaaaa cggtatagct gatgaatcgt acagataaca aaagaagtaa aggtataagc
     9601 cgttgctgta ttcccatgga gcaacgtgat tttttcttct ggaaaaacat aacgatagcg
     9661 taaaaacaat tcattacaaa catcgactcg cggtgtacaa atcgctactc ttcctccagc
     9721 ttttaaagtt tccactaaaa taggataaag catctctgtt ttccctgcac ctgttacagc
     9781 ccaaataaga tgatgtcttt tttctgaatg gcaaagtatt ctctttgaga tttccgtttg
     9841 tttttcagta agctgtcctg tccaaaaaaa gacgacttct cgaggttttg cttcaggctg
     9901 atcaaacaaa tataaatctt gttgagtgtc gcatcgccct aaatatagac aatgtgtaca
     9961 aaaataaccg taatctgcaa gctgtccatc aatgacggac gtcctcattc cacagcgatg
    10021 acaaagaatc cagttgccca aacgttccac tgctgcgatc ttctcataat ttggtaaatt
    10081 tacctcctca cagtctattg cttcaatatc tctcgtcaga agccttctac cccaaaaatg
    10141 attcatattc ttcgccctaa aaaaatatac gaaaaaacaa gcgatctatt ttttggatcg
    10201 cttgttcgat agttatactg ttctatttat tcacctatga gcgtgattgc ttgatctaac
    10261 actttttcgc cattcatcat accataatca gccatattga tgacatctaa aggaatgcct
    10321 tttggttcca gacgtttctc aaaatcagct ttcataaagc gaacttgtgg gcctaataac
    10381 aacacatcca cgtctttatt tgcaagattg ttatctgcgt cagaagcgga cacagcgaaa
    10441 atatccgagt cgattccttt tgcttcagca gctttttgca tttttgttac tagtaaactt
    10501 gtgctcatac cagccgaaca tacaagcatg attgtttttt tagccatact tttctctcct
    10561 ttttaaaaca aactatgcaa acgttttctt cgcgtctgtt actctattat ataaaaagaa
    10621 agaaatttgt caacgttttc taaacgcggt ttatttaatc aaatagccaa ctagatttca
    10681 ctaatttatt gaacacgaca tattctttgt ccaaattgat gacaactact tcatagcctg
    10741 catttatcca gccataggca cctttttcag ggtgtttata atcatttgcc caccaatctg
    10801 tactttccct tgctgttttg ggaagtccgt tactgggaaa cagcagttcg tcaaattggg
    10861 taaaggtcaa cgttacttga gagccgccat tgttcttcaa ataatgcgta attgcatcgt
    10921 actttttcat tcgttcctgc atccccagat ccacctgcct ttgtctttat tatcgcatac
    10981 tacagcaatt gcgcaaccgt tacagtgttg aaacaaccta gaactttatt tatactgaat
    11041 aaagaaaatg aaaaaaagag gtgacaagat gatcgagcgc tatcgtacaa taaaagaaaa
    11101 tggacaaagt gagattgaga tcaaaaaatc acgctttatc tgttctctta gacgaataga
    11161 atcagaggac caagcaaaaa gtttcattca agaaataaaa aaagaacatt ggaaagcgaa
    11221 ccataactgc agcgccttca tcattggaga aaaaaatgaa atccagcgaa gcagcgatga
    11281 tggagaacca agtggaacgg cgggcgtacc gatgttggaa gtcctcaaaa aaaatcaact
    11341 gatcaatgta gctgcagtag tcactcgata ttttggagga acgaaactcg gcacaggcgg
    11401 attaatacga gcatacactc atgcggtctc tcaagcattg tctgacattg gaatcgtaga
    11461 aggtaccttg caacaggaga tctctgtgat gatcacgtat ccgcaactag gaaaattaca
    11521 gaattatctt gaactccaac atttttttat aaaagaaatc caatatactg atcaagttag
    11581 cgtcatctgt gctgtagatg aaaaaagtgc tgaccacttt aaagaatcca tcattgagct
    11641 gctgaacgga caagtccaat ttgaagaggg ttctgtttct tatcacgagc aactgataac
    11701 tgatcatata taaaaacagg tgattctgat tctcttttat ttctaataag gaatcaggga
    11761 tcacctgttt tttatttttc caaataaaac tcaaaacgat ttcctacgta ttgcgtacgc
    11821 acatactcaa acggcgtacc gtcttcaaaa taagaaatct ggcgcaaacg aagaactgca
    11881 tctcctcgtt ttacctctaa atagtcagca attttctcag aagccaacac agcagtaatc
    11941 gtttgattcg ctggaccgat ctttttcccg cctttttctt ctagcgtgcg atacaacgat
    12001 tcagtaatct ctgctttgct gtagccatcg atcagctttt gtggaacact ggcaacttca
    12061 aaacaaatag ggacatcatc tgcgaaacga atacgttcca ttcgcaagat cgtgtcatct
    12121 ttactcaagt ttagtttttc catttcactt gagcttggcg aagtcaagaa ataagagatt
    12181 gtccgactag acggtactcg attttgtgat tccataattt ctgtaaaact tgttgcacca
    12241 gacatttttt cttgtacttt ttctcgcgca acatacgtac ccgaaccgat ctttctttct
    12301 aagattcctt catctgccaa gttttgaatt gcttgtctca acgtcatacg gctgacattg
    12361 aatttcactg ctaattctct ttctgaagga agacggtctc cgactttcca tataccctgt
    12421 tcgatttcat tttttaattg gtcatgaatc tgtatatata caggtaattg attcgacata
    12481 acgtacaccc gtcctttcgt ttcttctatt ataactggta tatacctgat tcacaactga
    12541 aaaagataaa ggtttctatc catcaacaga ggatttagac acacatcgtt ctccctactc
    12601 ctattgtcag aaacaatttt ttagatatgt tcaacctaga aaataacaaa agaccctaaa
    12661 atcgatgaaa atgccgactt atggatcttt tgttattatt agcggttttt agccatccat
    12721 ttttttgtat gattcgttgg gcgttttgat tttacctgga ccgcttttcc gccttttttc
    12781 ggaatattca cttcaatatt gtatttttct gatttgacca aatcatataa cttttcagct
    12841 gcttcgtcta ctttttcttt ccatttcatg tggatccctc caattcttgt agttaactta
    12901 actctatctt aattcagcag aatcttcaag gaacaaggta gcaaagcaaa aaaaagagct
    12961 ctcgaactaa c
//
LOCUS       DACECZ010000077        12854 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.077, whole genome shotgun sequence.
ACCESSION   DACECZ010000077 DACECZ010000000
VERSION     DACECZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.077"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            143..1318
                     /gene="mltG"
                     /locus_tag="IWO35_001923"
     CDS             143..1318
                     /gene="mltG"
                     /locus_tag="IWO35_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ0316980.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            1454..1933
                     /gene="greA"
                     /locus_tag="IWO35_001924"
     CDS             1454..1933
                     /gene="greA"
                     /locus_tag="IWO35_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ0316981.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            2102..2833
                     /locus_tag="IWO35_001925"
     CDS             2102..2833
                     /locus_tag="IWO35_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ0316982.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            2830..3897
                     /locus_tag="IWO35_001926"
     CDS             2830..3897
                     /locus_tag="IWO35_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0316983.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            3904..4536
                     /locus_tag="IWO35_001927"
     CDS             3904..4536
                     /locus_tag="IWO35_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0316984.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            4676..5341
                     /locus_tag="IWO35_001928"
     CDS             4676..5341
                     /locus_tag="IWO35_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ0316985.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            5369..5662
                     /locus_tag="IWO35_001929"
     CDS             5369..5662
                     /locus_tag="IWO35_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ0316986.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(5730..6677)
                     /locus_tag="IWO35_001930"
     CDS             complement(5730..6677)
                     /locus_tag="IWO35_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0316987.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(6855..7658)
                     /locus_tag="IWO35_001931"
     CDS             complement(6855..7658)
                     /locus_tag="IWO35_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ0316988.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            7804..8718
                     /locus_tag="IWO35_001932"
     CDS             7804..8718
                     /locus_tag="IWO35_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0316989.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            8766..9236
                     /locus_tag="IWO35_001933"
     CDS             8766..9236
                     /locus_tag="IWO35_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ0316990.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            9321..9929
                     /locus_tag="IWO35_001934"
     CDS             9321..9929
                     /locus_tag="IWO35_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ0316991.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            10063..10263
                     /locus_tag="IWO35_001935"
     CDS             10063..10263
                     /locus_tag="IWO35_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0316992.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            11017..12243
                     /locus_tag="IWO35_001936"
     CDS             11017..12243
                     /locus_tag="IWO35_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ0316993.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(12351..12821)
                     /locus_tag="IWO35_001937"
     CDS             complement(12351..12821)
                     /locus_tag="IWO35_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ0316994.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT         4448 a         1962 c         2606 g         3838 t
ORIGIN      
        1 ttgataacat ttattttaat cttatagtaa tggatttact acttacattt ttgatagaat
       61 atttaaataa aatcattcac gccaattcaa atatcatgta taatgaataa agttacgaac
      121 ttgatagtaa ggaggagttg ttttgtcagg aaacaaacaa cacccaagta aagataaaaa
      181 agaagccagt aaggcagcta cagcagatat aatcgacaag cggagaaatc tgcgtaaaaa
      241 agaagataag attgtgagga agatcatttt agtgattgct cttacattat tgattattgg
      301 cggatttctt ggatttaccg tgtatcgtta tgtagatagc gggttgaagc ctttagataa
      361 atcagatgac caattagtcc aagtcgagat cccgagtggc tcttctaaca agcagattgg
      421 tgagattttg gagaaggaca atattattaa aagcgggatt gttttcaatt attatacaaa
      481 atttaaaaat ttaactggct ttcaagctgg ctattatcag ttagcgccta atatgactct
      541 tgacgagatt ggcaaacagt tgcaagaagg cggtacttct gagccaacaa aagttgcaga
      601 tgggaaaatt gccattccag aagggtacga tatcgatcag attgctgaac gggtagcgaa
      661 ggtaactggt aaagacaaga aagaattttt agatttagtg aatgatgaga ctttctttaa
      721 cgagttgcac caaaaattcc cagaactgtt ggaaagtgca tctaaagctg agaacacaaa
      781 ataccgtttg gaaggttact tattcccagc cacatatgat tattacacga gtacttcgct
      841 taaggatcta gtgattgaaa tggtgaacaa gacgaatacg gtcatgcaaa actattattc
      901 agctatcaag cagaaaaatt tgactgtaca agaagtttta actctagctt ctctagttga
      961 aaaagaaggc gtaaaagaaa atgatcgtaa aaacattgca caagtcttct ttaaccgaat
     1021 caaagcaaat atgccacttc agtcagatat ctctgtttta tacgcattag gtgaacacaa
     1081 agaattagtg acgtatgaag atacagctgt agattcacca tataatttgt ataccaatac
     1141 cggctatgga cctggacctt ttgacaatcc aagcgaagag gcaatcaagg cagtattaga
     1201 accagcagaa aatgactatt attatttcgt agcagatacg agcactggaa atgtttattt
     1261 cgctaaaact tatgaagaac atatggaatt agtggaaaaa tatgtaaata attcttagaa
     1321 aaacaggctc tagacattga tcatttttag aaaaacattt acatttgaag aaaagcgtgg
     1381 taatcttttt tggactatat tcctcaaaag attacctttt gcggtatttt atcgaaaaaa
     1441 ggagacgtta cacatggtag agaaagttta tccaatgact ctagaaggta aagaaaaatt
     1501 agaaaaagaa ttagaagaac taaaaacagt caaacgcgga gaaatcattg aacgaatcaa
     1561 aattgcccgc ggtttcggag atctttccga aaactctgaa tatgaatcag cgaaagatga
     1621 acaagcattt gttgaaggaa gaatcacaac aattgaaaat atgattcgtt ttgctcaaat
     1681 catcgataac gacggtgtag atgcagatga ggtatcaatt ggtaaaacag ttacattcat
     1741 cgaactacca gatggagaag aagaagagta cacgattgtc ggaagcgctg aagcagatcc
     1801 gttttctggc aaaatttcca atgattctcc tattgcgcaa gcattgattg gcaaacgtaa
     1861 aaatgatgaa gtaactattg ctactccagg tggcgatatg ttagttaaaa tcgtcaaagt
     1921 agaacaagcg tagtctgact gaaagatcta gagagttcat cttactgatg atacactcta
     1981 gatctttttc tctttgtgta ttttagattt caacaaacga tcttacatct aaagtcctat
     2041 agccaacagt taaaaaaata ggtatgatga aatgagaatg tgtcaggagg gagaaagagc
     2101 gatgaatagt tcttggcgtt tttttgtggt agttgaagca ctactgctgt tatttgctgt
     2161 ttggcaaatc gtaaacaata ctggactttt gcttttagtg atattcggta ttttcaatat
     2221 atatctagct atgcgcaaac atcctcgtac taaatttcaa aattttcaat tgatattagg
     2281 aagtttggtc atttttttta gcttagtcaa cagtcctgct ttatggatga tggctgtatt
     2341 tgccgtttta tttattggtc tgaaaggtgt agagatttca gggatagatt taaccaaaaa
     2401 tgctttttgg cgaaagaaac agatcatgat gatccaaaca gagcaattaa aaacacataa
     2461 taatgagcgg aaaagacagc aattattcgg taaccaacgt attggaaacg acgtctatga
     2521 atgggacgat atcaacatcg ccgtcatttc tggtgatact atcatagatt tgggcaatac
     2581 gcttttgcca aaagatgata atattgtgat tgtaagaaaa gggataggca gaacacggat
     2641 cttagtacct ttgggcgtag ccatccgatt agagcatgcg acattagtag gaaatgtctt
     2701 gtttgaagaa gagcaatttt ctttgaaaaa tgaacaaatc aaaatctaca gcagtgatta
     2761 tgatgaaaac cctcgtagat tgaagattat tactaatacc ttgattggag atgtcgaggt
     2821 gatacgaata tgatgggaaa aatatccaga gcgatgctag ctgtttattc ggggattgcg
     2881 gctttcctta ttattctatt ttcacttttc acttattttt atgccagcaa tcaaagtcat
     2941 tggtggggag aattactgcg tgcacgttta ttatatgttc cgcttatctt ccatttgtta
     3001 gccatttcct taggtgtagg attaattgtc tttctattat tatcacttat tcaaaagaca
     3061 aaatacggga aaatcgaaga aaagctgcgt gcactttctt ctggcaatta tgaatccaaa
     3121 ttgttgcttc ttccgatccc aagtgcatcg gacgatttat acatcaaaga tattgataag
     3181 gaaatcacta agataaaaga aaaaatgatt gaagtatcta gtgaattaca aattgtaacg
     3241 agccgtcccc aatatgtaga tggacaaacc aaagaagaaa ttttagaatt agaaagacat
     3301 cgattagccc gtgagctgca tgattcagtt tcgcaacaat tgtttgcagc aatgatgatg
     3361 atgtcggcat tgacggagca agcagaaaaa agcgagacac cagaaatgtt tcgcaagcag
     3421 ttgaaaatgg tagcagagat catcaacgct tcccagtctg agatgcgtgc gctgcttctt
     3481 catctacgtc cagtcaattt agaagagaaa agtttaaagc aaggtatcga gcagttattg
     3541 aaggaattgc aaaataaaat ccagatttcg ctgaaatggg atgtagaaga tgtaaaacta
     3601 tctagttcca ttgaggatca cttgttccga atcgttcaag aattgttatc aaatacatta
     3661 agacatgcta aagccaatga attagaggta tatttgcaca aaatagacaa taatcttttg
     3721 ttacgtatta ttgacgacgg aacagggttt aatatgaatg aaacgaaaac gggaagttat
     3781 ggattgaaca atatcaaaga acgagtagct ggtattggtg gaacagtaaa aatcattagt
     3841 tttaaaggtc aaggtacaag tgtagaaatc aaggtccctt tgatgaagga ggcatagata
     3901 aggatgataa aagttttatt agtagatgac catgaaatgg tgcgcttagg cgtctcttct
     3961 tatttatcta ttcaagaaga cattgaagta attggagaag ctgaaaatgg acgacaaggc
     4021 tatgagaagg cgatgacact tcgaccggac gtcattttaa tggacttagt catggaagaa
     4081 atggatggta tcgaatcaac aaaagcaatc ttaaaagatt ggccagaagc taaaatcatt
     4141 atcgtaacca gttttattga tgacgaaaaa gtttatcctg ccattgaagc aggtgcagca
     4201 gggtatttgt tgaagacctc taccgcacat gaaattgctg atgctataag ggcaacgcaa
     4261 cgtggcgagc gagtattaga accagaagtg acaacaaaaa tgatggaaaa aatgagtcgg
     4321 cgaaatgagc ctgtattaca tgaagaacta acgaatcggg aaaatgaaat tttaatgctg
     4381 atttctgaag gaaaaagtaa tcaggaaata gcggacgaat tgtttattac actgaaaaca
     4441 gtaaaaacac atgtttccaa tatattggct aagctagaag tggaagaccg tacacaagcc
     4501 gctatctatg cattcaaaca tggtttggtg aaataaaaac gatgataaag aggataatca
     4561 aaagctgaga aaataactat tatttttctt ggctttttta caataatgat gcttgctttt
     4621 gtttttgtaa aggctttgtt atactgagaa aagaagatac taaaggagga aaacggtgaa
     4681 acaaaatttt gcaattgtag gtcttggacg atttggcgga agtatctgcc gaacactgat
     4741 cgaagctggt caagaagtac tggctattga tagtaatgaa gatcgggtca atgaatatat
     4801 gaatatagcc actcatgctg ttgtggcaaa tgcacaggat gaaatgacac ttcgatcatt
     4861 aggcataaga aatttcgatc atgtaattgt cgctattgga gaagatattc aagccagtat
     4921 cttagttact ctgatggtaa aggaaatggg cgtaccaaat attttagcaa aagcccaaaa
     4981 tgagtatcat gccagagtac ttgaaaagat cggagctgat cgcgtcgtcc atcccgagcg
     5041 agacatggga ataaggattg cgcataattt agtgtcaaag aatattttag attacctaga
     5101 gttgtcagat aaatattctt tagcagaaat ccgagtctcc aatcctaaat ttttcaacaa
     5161 aacattagct gaattaaatt tcaggcaacg ttttgactta acagtagtcg ccatacgaag
     5221 agtagatcaa acggttatcg cttctcctgc agcagatgaa tatgttcgtg aaaatgataa
     5281 tctactggtt attggagaga caggcgatgt ggatattcta gacgataaga tgaatcaata
     5341 gaaagaaaag agaaagtagg ttgttgggat gaaattaacg gtaacgccaa aagcacaaaa
     5401 gtggtttgac tcagaaattg atctgccaga aacttacgga atacgttttt ttggaaaagt
     5461 ctacggcaaa acagaggttc atgatggttt ttcgattgga atgtcagttg aacagccaga
     5521 acatcctgta aagaaagtgg aaatggatgg gacacttttt tttatcgagg aagcagatga
     5581 atggttcttt aaaggatatg atttacttgt tgattatgat cccaacctgg aggagccaac
     5641 ctatcacttt aaaaaacaat aaaaaaagag agggtgacac aatttttgtc accttctctt
     5701 ttttcttttg aaaatattgt tttcagctat taatatcctg cagacaattt tacttgattg
     5761 gtttgaagct catgggtggc aaagtagctt ttcagattgg ttaaaaaaat agccattaat
     5821 ttcttttgaa aatcaggtgt caaacctgca atgtgggaag taatcagtac attgtccatt
     5881 ttccaaagag gatgatcttg tggaagaggc tcttcctcaa aaacgtctaa tgctgcaaag
     5941 gaaagttgct ggttatttaa agcctgcacc agatcttgtg tcttaacaga agcaccacgg
     6001 ccaacattga tgaatacaga ttttggatca aagtgttcaa acatatgctg atcaaagagc
     6061 ccagttgttt cctccgttag aggtaagata ttgactacga aatctacttc aggaagaact
     6121 tcctttagtt gatttaaaga aaaagtcttt tccattcctt ctgctggatg accggatgta
     6181 ttgattccat agcagttcat gtctaatgct ttgcattttt ttgcaatcat ttttccaata
     6241 tggccagttc caatgattag tatttttttc ccttggatag attggtaatg gaccgatgaa
     6301 cctaaccatt tcatgtcact ctgtgccttt tgtgcttgaa aaagaccacg agaataccca
     6361 aggatgattc ctaagacatg ctccgtaatc gattcactgt ggatgccact accatttgaa
     6421 agtaaaatat gtttctttga aaaagtctca aggggtagct gatcaacacc agctgagata
     6481 gattggaccc atttcaaggt agaagaggct aatagttttt cttggtagtc tgagttccag
     6541 cccaaactga tctctacatt ttgtagatcc tctggttgta attctgtttt aaagatataa
     6601 ccaggcgcta tagtatggat ttcttcgata aattcatcac gaaatggacg atctgcataa
     6661 atgattcttt ctgacatagt taatttcccg cctttcttta ctctaagtgt agcgcataca
     6721 agaagaactt tgaagcgcct tgcttgtttt ttgagaaaga aatatttttc aaataaaaaa
     6781 ggggctgtga caaaaaaatg tcacaacctc taaaagggtc tattgaagta gaaataacga
     6841 gttgtcttta tttcttaatt gatcgatatt tttgcccatc ctttgacagc ttgagcaaaa
     6901 ataaagattc ctgcaaaagc aatgaaggcg ctagtgtcaa aagaaggaag aaagaccatc
     6961 aagattgttg caattatagt aggcaatgaa attgaataga tcaatgtttt taaacaatca
     7021 agaaaagtag cttttgttag tcgaaagcgt gcatagagaa aagcagcaat gttggtaaat
     7081 agcaaagtga tcagcagatt cagaaaacta ggataaatag aaaatataaa tgtcaggact
     7141 tttatccaaa gtggtataga tgcttcactt aaaaaagaac ggagctgctt acctgttaag
     7201 ttttttaaag attcattggt gtagggaagt tctaattggt tattgttcaa caaagcagaa
     7261 gtcgtgcctg tattaggaag agctatgata agtttgtttt ttaacatccc gacactaaca
     7321 aaattcccca tcaaatcagt agagatgtcc tgttctgttc gttttccctc tggatcaaat
     7381 gtgaagataa tggagtttgt ttggtaaatg aatccttctt tttcttcggt ttgaagctgc
     7441 ccgttttgga ttgtaaaatc aggaatgcga gtagcaatct tttgcccatc caattgaatg
     7501 ttgtttaaga cttgaaaaac ttgaaaagag attggcaaag ccataattat actcaaaact
     7561 aaaagataga caatggcttt ccaaaatggg acatttctcg cctcctttag gtctttgaat
     7621 cgtataaagg aactgataat taattgtttt gttgtcataa gtactcctca tctagtagtg
     7681 tcatcacttg caattctagc gaagtatcaa gtgaaataca agtgctttaa ggaaacagct
     7741 gaatgattgg tgaaatatca aaaaaatgtt attcttgtct aaagactatc caatataaat
     7801 aaaatggagg aaggaatagt gcctacacag ccacagagat catcaaaaaa actatttgga
     7861 gtagtggttt tgaatgcggt gatcacttta tcagaattta ttggagggat tatgtcaggg
     7921 agtttgtctc ttgtttcaga tgctttccat aaccttagtg atacattggc aattattttt
     7981 tcttacgggg cacaaaaaat ggcccgaaaa gaagcaaata aaaaacgtac gtatggctac
     8041 cagcgtttag aaatattgag tgctttcatc aactcattca tattgatcat attatccttg
     8101 tttttaaccg ttgaagcatt caagcggttc acttcaccag aaaagataaa tagtcatttg
     8161 atgctaacag tagcagttat tggattgctc gcgaatttgt tctcagtatt attattacga
     8221 caagaagcag atgaaagttt gaatataaaa tctagttatt tgcatttgtt aagtgataca
     8281 ctgtcttcgg taagtgtgat tatcggagca attttgatcc gttttttcga tatctactgg
     8341 atcgatccag ttattacact gatcatttcg atatatatat tgatagaagc gataatggtg
     8401 atcaaaaaag ctgccgctat tttgatacaa agtgcacctg ctatcgacta tgaaaaaatg
     8461 gaacaagaaa ttgaagcaat cgatggggta aaggatgttc atcatgtcca tatttggcag
     8521 tatagcgaaa agataattat tttcgacgga catattgatt ttgaagatca gctactaagc
     8581 gagattgaaa agatatatcc tcgtataatt tctttattaa aattaaaata tggtattaca
     8641 catgtgacga tccaagcaga gacaaaggca aaggatcaaa aaaaattaat ttttttaaat
     8701 aaagatgaaa gttattgaaa tatagagtat gataaataag aatttttaat aaggtaggtt
     8761 ggctcatgaa gcagtatcga cagttaaaag gatatttaga agcaaaagga tattgggaga
     8821 tactagtcta tctctttttt ggtggtttgg ctacgattgt aaattttgtt agttttgctc
     8881 tcgcacgcca atattttgat ttaagtatgg ccttatccaa ttctatttcg tggttttgtt
     8941 ctgtattgtt tgcttttgta acgaacaaac tttgggtatt ccattcaaaa tcaccgaatt
     9001 ttacacatgc acttattgaa tgcggtaagt tcttcttcta ccgtatcctt tcttacggat
     9061 tggacatggg ggcaatggta ttgttgatca atgttatgaa cagcaatgag tatgtggcga
     9121 aaatcatcac tcaaattatt gttatccttg cgaactatat tttcagcaag ttgtttattt
     9181 ttaaagaaac tgaggttttt gaagaagaag taggtcaaag tgaaaaagac gactaggcat
     9241 aacaattgaa gaatttcctt ctctttgtta ctctttgtaa tgtagacatt attaagaaaa
     9301 cattgaggag gaattttttt atgacttaca cattaccaga tctaccttat gcatatgacg
     9361 cattggaacc ttatattgac gaagaaacga tgcatctgca tcatgataag catcacaata
     9421 cttatgtgac gaatttaaat tcagcaattg agaaataccc agaattaggc gaaaaaacaa
     9481 tagaagaatt attatctgat atggacgcta ttccaacaga tatcaagaca gctgtacgta
     9541 acaatggtgg cggacatgct aaccattcat ttttctggga aatcatggca ccaaatgctg
     9601 gtggcgaacc tacaggagaa ataaaagaag cgattaatga agcttttggt gatttttctt
     9661 cttttaaaga agaattcaaa aaagcagccg ctggacgatt tggttctgga tgggcttggc
     9721 ttgtaatgga aaatggaaaa ttagctatta cctctactgc aaatcaagat tctccattga
     9781 tggaaggaaa gacaccaatt ctaggtttgg acgtttggga acatgcttat tatttgaaat
     9841 ataaaaatgt ccgaccagat tatattgcag cattctggaa tgttattaat tgggatgaag
     9901 taaacaaacg tttcgaagca gcaaaataac catttcctcc gtcatatcag tcttaaaaat
     9961 aatttggaaa ataaaagagc gaaagcttgc tagcgttttc taaattgggt ataatgttct
    10021 tgcaaggtta cttgcaagaa cttttttgga ggatatacat ttatggaaca cggaacggtt
    10081 aaatggttca acaatgaaaa aggatttggg tttattacag tagacggcgg agatgacgta
    10141 tttgttcatt tctcagcaat tcaaggagac ggcttcaaga gcctagaaga aggtcaagaa
    10201 gtagaattca cgattgttga aggtgcacgc ggacctcaag ctgctgaagt gactaaatta
    10261 taataatttg ttgaataaag acacgacttc ttagagagtc gtgtcttttt gtctatttat
    10321 gaagttttta gattttttgg ctaagtaaat gcatttgttc acttgacatg tcctaactaa
    10381 ttatcaagca aggaacattt tcttcgttag taatggattt taaagaaatt tcagtttttg
    10441 gatgttgaat aaaagaccat ttgtgaaacc actagtatac atactcatta ccatgtcaat
    10501 tctattactt taaaagaaaa tcgttttact ttgaagagga gcatatctat ccattaaaag
    10561 atgatggtaa tgcaatcact agttcgagtc attttaagag ccatgcagtg agctggaaat
    10621 aaaacgaata gaagatgggt ttaacaaaag gaccagtcat aggaagaaat tgtcgtgaaa
    10681 tcgaatgata atctgaacgg taggcaaaga ctgttaaaaa gatgggaaag gtatgtgcga
    10741 tcattttact gcacgattat ttggaaaata ccttgttgtt cgctttttct ggaaaaaata
    10801 cataaaaaaa gataaaacga ttgcgaactg cgtgtattcg gtctttcgat ctttttcacg
    10861 gtatggtatg atagttaaga attttgagaa attttaaaaa ataataatcg ttacacaaaa
    10921 atgtttttta aagttttctt atgattaaaa agtgaatttg ctgattgaac agttatatga
    10981 gacaatattt tagaaaatgt gaaacagagg tgtattatga agcgatggca aaaagttcta
    11041 ttaaccttgt taggggtatt cgttttgctg attgccggat tatcagcata cggaatcaga
    11101 tttatgggag aagcgagcca aacagtaaac aaaatttcta aaaactctaa tcgaatatcc
    11161 tccaaacgtt ctgagagagt aagcattgat gacaaagaac ctttttcggt tttgctatta
    11221 ggtttagata ccggtggatt aggaagaacg gaacaaggac gttctgatac gatgatggta
    11281 gtaacagtta atccgaaaca aaaaaaatca acgatcatca gtttggaccg agatatttat
    11341 acgaatatcg taggttatgg aacggtagat aaattaaatc atgcctacgc atttggtggt
    11401 gtcgaaatgg ccatggattc aattgagcaa ctgctcgata ttcctattga tcattatgta
    11461 acgatcaatt tggatggcat ggaagatttg atcaatgcgg tcggtggcat caaagtgaat
    11521 aataaaattg actttacttt ggatggtgtc catgtaccag taggtgaaca aacattgaat
    11581 ggagaaaaag gtttagccta ttctcgaatg cgacacgaag atccagaagg cgatatcggt
    11641 cgacaacgtc gtcagcggga agtagtgacg aaaatcgtca ataaagtttt aagtttagat
    11701 ggcgtaagca attatagaaa aattttaaaa gcagttgaga acaatgtgac gacggactta
    11761 gattgggatg acatgcttga tgtagcgacg aactacaccc cggcgtttga aacaatcaaa
    11821 caagaccagt tgcaaggcga aggacaaatg atcaatgata tctactatca aattttagga
    11881 aaaaatgatc ttctttcgat ccaaaatgaa ctgaagaaac aattaaatat aaaaacaagc
    11941 gatacattac ctaatttgaa aaatgagaat gcttcaatga tgttctttga tgattcggaa
    12001 gatggcgatt cgacaaacgg tgatggatcg aatagtgaag ggtacaccga ttattcttcc
    12061 tattcagaaa acacatatga tcagtaccaa gatccgaata cctattatca gcaaacagaa
    12121 gatacaagtc agtactatac accaagtcaa gatactcaaa gttatgatcc ttacgctgga
    12181 caggaacaaa atggttatgg ttatgaccag aataatgagt atggaacaac aagtgggtac
    12241 tagaaaaaaa tagtttggtt aataaagagt gccacctaaa ttagacaaaa aatctaactt
    12301 aggtggcact ttttctatgt gttaaattac aggacgtctc tttttagcgc ttagtttaaa
    12361 aaataaattg aataattcaa tagagttgcg aatagtaccc ataaataata aggaatcatg
    12421 agccacattg tcaggcgatg atgcagccac aactgataca taagcactga caagaaaata
    12481 actaaaagtg taatatcgat tgctgcaatg aaaatatttc ttaaattaaa aaatagagca
    12541 gaccatataa aattaaaaat gaactgaatc acaaaaagac cgacaagatt gtttcgctgt
    12601 tttttgttgt tgtgattaag taacaaataa agagaaatac ccatcaatat atacagtaag
    12661 gtccacatcg gcccaaatat ccagctaggt ggtgcaaatg gtggaagttg caaagaatag
    12721 taatattttc cgggatttcc gctaagcaac cctgataaaa agcctaatat gacaattcct
    12781 ataacacaaa cccaaaaacg aatatctttt atcatcttca ttttactaac tcctttctat
    12841 aatattagtt tacc
//
LOCUS       DACECZ010000078        12290 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.078, whole genome shotgun sequence.
ACCESSION   DACECZ010000078 DACECZ010000000
VERSION     DACECZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.078"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(180..737)
                     /gene="frr"
                     /locus_tag="IWO35_001938"
     CDS             complement(180..737)
                     /gene="frr"
                     /locus_tag="IWO35_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ0316995.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(740..1462)
                     /locus_tag="IWO35_001939"
     CDS             complement(740..1462)
                     /locus_tag="IWO35_001939"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ0316996.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAHMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1598..2479)
                     /locus_tag="IWO35_001940"
     CDS             complement(1598..2479)
                     /locus_tag="IWO35_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ0316997.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="IWO35_001941"
     CDS             complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="IWO35_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ0316998.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3719..4198
                     /locus_tag="IWO35_001942"
     CDS             3719..4198
                     /locus_tag="IWO35_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ0316999.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4415..5506
                     /gene="phnW"
                     /locus_tag="IWO35_001943"
     CDS             4415..5506
                     /gene="phnW"
                     /locus_tag="IWO35_001943"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ0317000.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5499..6284
                     /locus_tag="IWO35_001944"
     CDS             5499..6284
                     /locus_tag="IWO35_001944"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0317001.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6630..8321)
                     /locus_tag="IWO35_001945"
     CDS             complement(6630..8321)
                     /locus_tag="IWO35_001945"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ0317002.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8743..9690)
                     /gene="arcC"
                     /locus_tag="IWO35_001946"
     CDS             complement(8743..9690)
                     /gene="arcC"
                     /locus_tag="IWO35_001946"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ0317003.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9805..10824)
                     /gene="argF"
                     /locus_tag="IWO35_001947"
     CDS             complement(9805..10824)
                     /gene="argF"
                     /locus_tag="IWO35_001947"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ0317004.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10915..12144)
                     /gene="arcA"
                     /locus_tag="IWO35_001948"
     CDS             complement(10915..12144)
                     /gene="arcA"
                     /locus_tag="IWO35_001948"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ0317005.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3380 a         2549 c         2199 g         4162 t
ORIGIN      
        1 acttaaaaga ttcccactat cttaagtatt atagcaatat ctaactgttt ttcctatgaa
       61 atcattgttt ttttatttaa aaactgaata attttttctt tttttaattt ttaaataaaa
      121 gaaatataaa taaaaacaga ggaagcggaa tgatccgccc ctctgttttt atgattttat
      181 taaacttcca ataattcttt ttctttatca gcagcgatct tatctacttc tttaatgctg
      241 ttatctgtca atgtttgtac gtctttttct aatccgcgca aatcatcttc ggtaatatca
      301 ccattttttt gttgtttttt atattcatcg atcgcatcac gacggatgtt acgaatcgcg
      361 atttttgcat tttctgcttc ttttttcaca tctttagcta attcttttct gcgttcttct
      421 gtcagttgcg ggataactaa gcgaatgaca tttccatcat tggttggact gattccgata
      481 tcacttgcca taatagcttt ttcaacatct tgaagaatgc tcttgtcaaa aggtgtgatc
      541 attaaaacac gtgcttctgg aatttggatt gatgccattt ggttcaatgg tgttggcgca
      601 ccgtagtagt tcacagtaat acggtccaaa aggctagcgt tcgcacggcc tgcgcggatt
      661 tgcccaagtt cacgttgaag attttgtgct gcttttttca ttttttcttt tgcttctgtc
      721 ataattgcat ctgccatttt tatttccccc ttacagttgt tccgatattt tcgcctaaga
      781 ttgcacggcg aatatttccg tgttcattta aattgaagac aagcagtgga atatcattat
      841 ccatgcttag tgaacttgca gttgaatcca tgacttgtaa tcctttggca ataacttcca
      901 gatgagtcag ttcttcaaat tttactgcat ttgcatctac tttcggatca gcagaataaa
      961 caccgtctac attgttttta gccatcagga taacatctgc gccgatttct gccgctcgta
     1021 atgctgctgt tgtgtctgtc gagaagtacg ggttgccggt accgcctgca aagataacga
     1081 tccgtccttt ttctaaatga cgttctgctt tacggcgaat atatggctct gcgatttgac
     1141 gcatttcaat cgaagtttgc acgcgtgtcg gtacacccac gttttccaac gtatcctgta
     1201 aagctaaggc attcataact gtagctaaca tacccatgta atcagcttga gcacgttcca
     1261 tgcccatatg tgctccgatt tgtccgcgcc agatatttcc gccacccaca acgattgcca
     1321 tttcaacgcc cagctcgtga acttctttga tttcttggat gatttctttg attactggtg
     1381 gtttgattcc aaatcctgca tccccagcta aagcttctcc actcaatttt aagactacac
     1441 gctgatattt cggtgtgacc atgttcattc ctccaatatt ttctattgcc attttaccat
     1501 acttagatga tttcgacact ctaagactgg tttttcttgt tttgcttaaa aaaagggagc
     1561 gcacaaaccg tgcgttcccg tctgttattc aacagaatta ttttttcact tggctcataa
     1621 cttcttctac aaagttatct tcgcgttttt cgataccttc gccaacttcg aaacgtacga
     1681 atgttttaac agttgcgcct tttgatgcaa cgtatttttc aacagtcata tcaggatctt
     1741 taacgaatgg ttggtccacc aaagcgattt ctgctttgaa tttcttcaag cgtccttcta
     1801 ccattttttc aacgatattc gctggtttgc cttcgttcaa tgcttgttct gtaagaacag
     1861 ttttttcatg ttctaattca gcttctggaa tttgtgattc gtttacataa cgaggattga
     1921 tagctgctac gtgcatagct acgtctcttg caactgtttc gtcagttgtt ccatctagaa
     1981 cagtcaatac ggcaatacgt ccgcccatat gcaagtatcc accaaatgca gcattgtcgt
     2041 ctttttctac aacttcaaag cgacggaagc tgattttttc tccgataact tgagttgctt
     2101 cgatcaagtc agattcaatt gtacctttat ctgttttgat tttcattgca gcttccatgt
     2161 ctgctggttt gttttcagca acaagttctg caatttcttt tactaaatct tggaacattt
     2221 cgtttttaga aacaaagtct gtttctgagt ttacttcaac gattgctgca gtgttacctt
     2281 tcacagcaac tgaagccaat ccttcagctg caatgcggtc atttttcttc gcagcctttg
     2341 ccattccttt ttcacgtaaa agatcaactg ctttttccat gtcgccttct acttcaacta
     2401 atgctttttt cgcatccatc attcctacgc cagtcatttc gcgtagttct tttaccattt
     2461 tagctgtaac gtctgccatg tttaactcct cctgtgtgtt tttcttttta aaaaaagctg
     2521 cttcaaagga gagtgaggct tgtcgcccag cctttgaaac agctccatca ttcaaaatta
     2581 ttctgctgaa gaattgtcgc cttcaacaac atctacgatt tcttcgattg atgttgctgt
     2641 gttttcttct gtgaaatctt cttcaactac ttgatcttca ccttggtttc cttcgatgaa
     2701 agcatcagcc atttttgaag taatcaattt aacggcacga attgcatcgt cgtttgaagg
     2761 gattactacg tcgatttcat ctggatcgca gtttgtatca accatagcta cgattgggat
     2821 atttaatttg tgtgcttctt gaacagcaat acgttcttta cgtgggtcaa cgatgtacat
     2881 tacatctggg attcttggca tatcagcgat accgcctaag aatttttcaa gacgttcacg
     2941 ttctttgttc aaaccagcaa cttctttttt aggtagaact tcaaaagttc catcttcttc
     3001 cattgcattg atttgtttca aacggctgat acgtttttgg atagtatccc agtttgtcaa
     3061 tgttccgcct aaccaacgat ggttaacgaa gtattgtcct gcacgagttg cttcttcttt
     3121 gattgcttct tgtgcttgtt tttttgtacc tacgaataat gctacgccgc cttcttcagc
     3181 aacgctcttc atgtaatcat aagctgcatc tactaattta actgtttttt gcaagtcaat
     3241 gatgtagatt ccgtttcttt ctgtgaagat atatttcttc atttttggat tccagcgacg
     3301 agtttggtga ccaaagtgta cgccggcttc tagtaattgt ttcatagaaa ttactgccat
     3361 gtttgtttcc tccgatttgg ttttattttt cctcttctta tcttctacct tgccggcaaa
     3421 ctttttcaag cacctaccga caatccaata agaatgtgga tttgctgatg cataaagcac
     3481 cctcagctat tatacagaca aaagatccgt atcgcaagag caaatatact tttctgctca
     3541 acattcaata gtttacctga aagataaaaa gaattcaaga aaaacagtag atttctcttt
     3601 gtttctttat tatccttttt ccataaaata attttaggta taaaagacga atattattgg
     3661 gcaatccttc ttttttttgt tatgatacat gtgaaatctt tcgtaaagga gaaagaaaat
     3721 gcgcaaagct caaagacatt tgctgattaa acaagtcatt gaagaattcc cgatccgtac
     3781 gcaagaagaa ctattaaaca aacttgggca ttatggcgtt accgccacgc aggcaacgat
     3841 ttctcgagat atccgtgatc taaaaatcgt gaaggcacct gatgaaaatg gcgtttcccg
     3901 cttcgtattg tttcgcgaaa aaggaactcc tgagactagg actgaggaag aggatcggct
     3961 ggttcaaatg attgaagaca ttgtattgaa agtcgaacga gtccactttt tgacgatcgt
     4021 caataccttg ccagacaatg ctcatttatt tgcatctgtt ttagatgaga tcaaacctcc
     4081 ctctattgtc agtacaattg ctggattcga tacgatcatg gtcatctcgg aaaatgagga
     4141 ggacgcaaaa cgagtagaag aatttcttca ccatccaact gaagtatcac ttttttaatc
     4201 agagcacgta ttcctaatat ggaatgcgtg tttttttatt ttcaatattt tccttattaa
     4261 ataaaccttt acactcttta caaaaataca tcatttttta ttcaattttt tcaacacctt
     4321 gattgaatca caataatcat catttatatt ggatgttaac aagagcagct tcttgctctt
     4381 tgtgtatttc aaaaaagaaa tgaaaggaaa tcatatgtat aaattattaa ctcctggacc
     4441 attgacgact tctgaaacag tgaaaaatgc tatgctgatc gatcattgta cttgggatca
     4501 agaatacaaa gaaattacac aatggataag aaaaaaatta ttggatcttg ctcaagtatc
     4561 ggaaggaata tacacaacgg tcttaatgca aggtagcggc tcctttgggg tagaatcggt
     4621 attaagttct gtgatcaggc cagaagatac tgtactgatt tgttcaaacg gcgcatatgg
     4681 acaacgaatg atcaacatgt gcgaatgtct gaaactgaac tataccgttt atgaggtatc
     4741 ggaacatcaa atcctccaag ctcatgtgat tacacagctg attgaagaga atccctctat
     4801 ctccgcacta gcaatggtcc atagcgagac tacttccggt attttgaata atttggaaga
     4861 gattgctgct gtcacacaag aaaaagatct tttgtttata gtggacgcga tgtcaagctt
     4921 tggtgggata ccgattcgtg tagccgaact agggattgat tttttgattt ccagctcaaa
     4981 caaatgtatt caaggtgttc caggcttttc ttttgtactt gctaataaag cggaactaga
     5041 aaagcgaaaa aatgtgagtc gaagtttgtc acttgatcta tatgaccagt accgcgtgat
     5101 ggaaaacgag aatggcaaat ggcgtttcac ttcccctacc catacggttt tggcttttca
     5161 acaagcgctg aaggaactag aggcagaagg aggaatcgaa actcgatatt cacgttatct
     5221 cgccaataat catgtactac gacaaaaaat gacagagatg gggttccagc ctttcattca
     5281 agaatatcag gggccattca tcacctcttt tctttaccct aaggactggc gatttgattt
     5341 taatcatttt tatcagtatt taaagcaaaa cggatttgtg atctatcccg ggaaaatcag
     5401 cgatcatgaa tgttttcgta tcggaaatat tggcgagatc tatccggaag atattttgga
     5461 actgacagca attatcaaag actataagga gaacgttaat gattaaaact gtaatttttg
     5521 attgggcagg aacaactgtg gactttggct gtatggctcc tgtccacgct tttagaaatg
     5581 cttttctaga aaaaggaatc caactgactg acaaagaaat ccgcgaaccg atgggaaaat
     5641 taaaatggga tcatatccaa cagcttcttt ccctcccttc tgtcaaaaaa caatggatag
     5701 ctatttatgg acaactacct caggaaaaag aggtggagga attgtatcaa caatttgaac
     5761 attatctgtt taaagatttg gtcaagcatt ctaccttgaa acccgacaca ttggaaacga
     5821 tcgaacggtt gagaaacgct gggatcaata ttggttccac gactggattt actgctccta
     5881 tgatgacaat cgtagcagaa accgcaaaaa aagaagggta ttcaccagac ttcatggcaa
     5941 cttctgaaga cgtcgaaggg tatggtcgac cgtttcctta tatgatccat aaaaacatgc
     6001 agtacttcaa aaacaagtcg gttgatgaag taatcaaagt cggagatacg gtctcagata
     6061 tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt aattgaaggt agttcgctaa
     6121 tggggatttc ttatgatgag taccaaaaat tagagatggg gaaaaaagat cgtttaaaac
     6181 agtctgttgc tcttagattc caacaagctg gcgctgatat ttgtgtaaac aatttaaaag
     6241 agttaacaga atacattttg tacgctgaca agaatccaga atgactttta tacaaaatca
     6301 gaggtcgtga aaaagctgta ctgcatcaag taataagaac agccaaacaa aaatagctcc
     6361 tcatattttt cgtttggtta ggcttattac cgcagatgca agcttttgaa caccgtttat
     6421 tcgaaaaaaa aagggagctg tgacacgact tttgtcacag tccccttatt tacaaacaaa
     6481 cagtgtttaa gagacaacct ctccactaaa cggtgttcat tcagctgact ctcggtattt
     6541 gctcaaaaaa cgataaaatt tgaaaagcta ttttctcttt tgtttctcta atactcggat
     6601 cagaacgctg atttcacatt ctctttttct tacatcttat ctggtgcatg aagtcctagc
     6661 aaacgaaggt cttctttcaa taatacagtc actgcataaa ctagagctaa gcgtgcttct
     6721 ttttgttcat catcagctaa aatcttcgta tgagcatagt atttgttgaa tgcttgtgct
     6781 aatttgattg catgtttcgc aatcactgac ggttcgtatt tttccccagc agacaagacc
     6841 gtttcagggt atttttgaac taatttaacc acttcccagc tggtatcgtc attcaatgca
     6901 tagtctgctg tttctgaagg gacaaatcct gctttttcca acaggctgac tgcacgagca
     6961 tgtgtatatt gcacataagg tccagtttct ccttcaaatc gaacaacttc ttccaggtta
     7021 aagtcaaatg tattcagtcg atcgtttttc aaatcgtgga aaatgactgc cccaacccca
     7081 acttgtttag ctacagcatc tttgttttcc aaatcagggt ttttctcgct gatttgttct
     7141 ttagcagatt ggattgcttc atttaatact tcttctaata agacgatctt tcctttacga
     7201 gttgataatt ttttaccacc ttgcgtgatc aaaccaaatg gaatatggcg catatcgtca
     7261 gaccagtcaa agcccatttc cttcaagaca gctttcaact gtttaaaatg atagctttgt
     7321 tcattcccta ctacatatag agattgtttg aagtcgtaag tacgtttacg gtacaatgca
     7381 gcagctaagt cacgtgtaat gtatagtgtc gcaccgtctg acttcttaat caacgctgga
     7441 ttcaggtcat aagcagatag gtcaacgatc tctgcacctt tatcctcgtt cagcaaatgt
     7501 ttttcttcta aaagtttgac gatttcatcc atcttgtcat tgtagaaagc ttcaccgttc
     7561 aacgaatcga aacgtacttc cagcagatca tagattttat tgaattcttt catcgattca
     7621 tcacggaacc attgccataa ttgaatagcc tcttcatcgc cttcttccag acgtttgaac
     7681 caagcacgag cttcttcatt taattctggt tctgtttcag ctacttcatg gaattggaca
     7741 tataaacgaa gcaattcatt gattggttct gctttaacag cttcttctgt accccatttt
     7801 ttgtaagcga cgatcagttt tccaaactgt gttccccagt cacctaaatg gttgatgcgg
     7861 attggctgat aaccgatttt ttccataatg aaaccgatcg aattaccgat aactgtcgaa
     7921 cgcaaatgtc ccattgagat tggtttggca atattaggag aagacatgtc gattggtact
     7981 gttccttgat tcccgatatt gctatcccca taatgttctt tttctttcac tactgtttgc
     8041 aaaacttttt tgctgataag ttctttattc atgaaaaagt tcaagtaagg tccgacaact
     8101 tcgatttttt caaagttggc agaatcgatt ttttctgcta aatctgcagc gatttgctgt
     8161 ggcgcttttc ggtaaacttt tgctaatgaa aaggctggaa atgcaacatc tccgtgttca
     8221 gctgattttg gattttctag taattgttcg acttgttcca gtgttaaatc atctttgaca
     8281 gcgtcataga cagcttttgc gacaagatct ttattattca tctttattcc tcctaaagtg
     8341 ctcccttgat tgttaaacaa aaaaaatctc tagcgacggc atacttagta tgccatcact
     8401 agagacgaga tttcccgcgg taccactcca attgtcctaa aaaaggacca gctccatctg
     8461 atatcggtag acgaccgtct gtttaaaaca gaattctcca agtgcgcttc gttgtatctt
     8521 cttcattcgg cttccaccac ttccgaattc gctagccaag aatgatacaa gtactctctt
     8581 gttcaacaaa tcttagtctt attaaacttt cttgaattat atcagaaaaa aaaacgaaag
     8641 gctagtcgtt tctgataaaa aaacaatcca gatggcctaa aggagaagaa ataccacctg
     8701 gatagctata aatcatttcg taaatacgaa agacttaaca aattaatcag ctacgataat
     8761 cgttccttct tcagaagcta aaagattctc gatattttct aaagaagtga tgattgcctt
     8821 agcgtttggt tttgcttcaa caaatgcaat agctgcctct actttcggaa gcatgcttcc
     8881 tggagcaaat tgcttttcat caatgtattg tttcatttct gaaacagtaa cagtttctaa
     8941 tttcttttga tctggttttt ggtaattgac atacacattg tctactcctg tcaatacgat
     9001 cagcaaatcc gcatcgatga tttcggctaa tttttctgaa gcaaaatctt tatcgattac
     9061 ggcttctcgt ccttctaatc cagtagctgt ttctacaaca ggaatcccac cgccaccaac
     9121 agagactgtg ataactcctt gatcgaccag cgtttcgatg acacgtgctt ctttaatcga
     9181 gatgggttta ggggaagcga ctacttttct ccatccacgt ccagcatctt ctttgaatgt
     9241 cacagtggag tcagcattca tttgttcttt ggcttcttct tccgtgtaga atggaccgac
     9301 tggtttactt ggatttttga atgaaggatc attttcatcc acaataactt gagtgactaa
     9361 agaaaccaca tctttatcga tccctttttc cttcagtact tcgcccatgg cattttgtaa
     9421 ccagtaaccg atagatcctt ctgtcattgc aacacatgta tctaaaggca ttgccggtgt
     9481 tttctcagaa tcagcagctt gctgctgaat caataggtta ccgacttgcg gaccatttcc
     9541 gtgggagatg atcaattcat ctccttgttc gataaatttc actaaatact ttgctgtttc
     9601 cattaacgct tcttgttggg cttttgcact tgcatccgta gacaagattg cattaccccc
     9661 taaagcgaca actacttttc gtttggccat ttcattcgtc ctttcttcag ttctaccgaa
     9721 ttttctagat aacaaaacga gccagcgttt tgtgattttc cctcaatgct gactcgtttt
     9781 ttcgtgagat gaatgtggtt atgcttaaac gcgagggata aacaagttac ctaatgttgc
     9841 tgccatgatc gctttgattg agtgcatacg attttctgct tggtcaaatt gacgagcata
     9901 tttgctgcgg aagacttcat ctgttacttc catttcagtg atgccaaagc gttctttcat
     9961 ttgttcaccg taaacagtgt ttgtatcatg gaaagctggc aagcagtgca agaagatcaa
    10021 acgatctgtg ttgtgtgttt tttctaccat ttccatattg atttggtaag gtttcaacaa
    10081 tttgatacgt tcttcgaatt tatcttcttc acccattgat acccatacat cagaataaag
    10141 gacgtctgct ccgtctacac ctttatcaac atcgtcggta atcatcaatt gtgcaccgga
    10201 tttttcagca aagccttctg ccattttgac gatttcttct tcaggttgta attcttttgg
    10261 tgcaacgata cgcatattcg ctcctaaaat tgcacctgtg actaataaag agttagccat
    10321 attgtttcga ccatctccgc agtaagcaac agtgatacct tctactgttc caaagttttc
    10381 ttggatagtt aaaaagtcag cgatcatttg tgttggatgc cattcatcag tcaagccatt
    10441 ccaaactgga acaccagaga attctgctag ttcctctacc attttttgac tgaatccacg
    10501 gaattcgatt ccatcaaaca tacgacctag tactttagct gtatcttctg tggattcttt
    10561 tttacctaat tggatatcat ttgcacctag atattcagga tgtgcaccta gatcgatcgc
    10621 tgcagtagta aatgcagaac gagtacgtgt agatgttttt tcaaataaaa gcgcaatatt
    10681 ttttccttct agatagtgat gtgggatacc acgttttttc aagtctttca agtgttctga
    10741 aaaatcgatc aaatattgta attcttcttt tgtaaagtct ttttctgcta ataagcttct
    10801 tccttggaat actgattctt tcatattaaa cattctcctt tagattgttt ttatttaaga
    10861 ttaaagagga aatcccgacg gcagctgaca gtaagaccgc cgtcaggaaa caaattactt
    10921 caagtcttca cggacaagtg gttggctcat gcagcgtgga ccaccacggc ctcgtgacaa
    10981 ttcacttgaa ttgatttcta acactttgat gccatagcta cgaagcaatt cgttcgatac
    11041 ataatttcgg ttataagtga ctacaacacc tggagcgatt gccaatgtgt ttgatccatc
    11101 attccattgt tctcgaggtg caacgatctc atctccatta ccagttggga tcagtacaag
    11161 atcatctaat ccaagagcag ctttcatcgt ttcgtgtaag ttatctgaat gagtaatctt
    11221 gatatcatca ccatctggtt cgattgtaaa gacatccatc ttaccgtctt tactttgaat
    11281 tgctggatgg atagtgaatt tatcgtgatc taccattgta aatacggtat ccaaatgcat
    11341 cattgcacgg ttgtttggaa ttttgattgc taacactttt tcaaatcctg aattttttgc
    11401 gaataggtgg cgtgctaatt tttctaaagc tttcgcatta gttcgttgag aaatcccaac
    11461 agctactact ttatcgctta agatcaattc gtctccgcct tcgatatgat ctggattttc
    11521 acgatccaac catacttcga ttcctttatt tgcaaatctt ggatgatgtt tcaaaatata
    11581 ttctgtaaac atcgattcac gacgacgtgc ttcaaaagtc atcttattga ctgtcatccc
    11641 atttcccatt gatgcggaag ggtcgcgtgt aaagtacaag tttggcattg ggtccatgta
    11701 aaatggatag tcatcgtctg ctgacagatc gtatagactg ctagaacgta cttcaatatc
    11761 ttttgtacga accccagcca tgattttatc taccatttct tgcgtttcca ttgatagaag
    11821 gaactcatga agaccctctc ttacagcatt cgatgcaata tgagattcat ccaacatttt
    11881 attcaagaat tgttctttga cattgcctgc gtcaatcgct tctgccgcta atttttccaa
    11941 gtaaagagtc tcgacgcctt cattttgcaa tgtctttgca aagttgtcat gttcttcttg
    12001 agcaatcgga agatatggaa tatcatcgaa caacagacga tccataatat ctggtgtcaa
    12061 attttccact tcttgtccag gccttttcag taaaactgtc tttaatttcc ctatttcaga
    12121 gaaaacgtga ataggtttat ccatgttgtg ttgcctcctt ctttttgatt acatgtatag
    12181 gatatcctcc ttttgtaatc actttcataa ttttgtctca ttcaaaatga gacattcctc
    12241 acattaatat tcgataatta aataattaaa cgtttttcta tttttaagcg
//
LOCUS       DACECZ010000079        12283 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.079, whole genome shotgun sequence.
ACCESSION   DACECZ010000079 DACECZ010000000
VERSION     DACECZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.079"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            140..460
                     /locus_tag="IWO35_001949"
     CDS             140..460
                     /locus_tag="IWO35_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317006.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="IWO35_001950"
     CDS             912..1112
                     /locus_tag="IWO35_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0317007.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="IWO35_001951"
     CDS             1259..4048
                     /locus_tag="IWO35_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0317008.1"
                     /translation="MKPRTYAVIDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="IWO35_001952"
     CDS             4095..4622
                     /locus_tag="IWO35_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0317009.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="IWO35_001953"
     CDS             4643..5833
                     /locus_tag="IWO35_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0317010.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="IWO35_001954"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="IWO35_001954"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ0317011.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8224..8898
                     /locus_tag="IWO35_001955"
     CDS             8224..8898
                     /locus_tag="IWO35_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0317012.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8895..9236
                     /locus_tag="IWO35_001956"
     CDS             8895..9236
                     /locus_tag="IWO35_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317013.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9266..9625
                     /locus_tag="IWO35_001957"
     CDS             9266..9625
                     /locus_tag="IWO35_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ0317014.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            10149..12230
                     /gene="cadA"
                     /locus_tag="IWO35_001958"
     CDS             10149..12230
                     /gene="cadA"
                     /locus_tag="IWO35_001958"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0317015.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
BASE COUNT         4080 a         2147 c         2659 g         3397 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcattgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaacggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ctatcatcat
     5341 caacggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa
     5401 tcctgtgatc atcaatggaa tgatcgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag caagatacag tggaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatattcg cttgtggcag aactgccggg
     5581 tgtttctctt gaaaagccac aaggagcttt ttatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctaaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatat gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttatgatga
     6721 tgcgatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtgat
     6901 ctgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacagactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggtgatg aaaaagctgt tctgcatcaa
     7201 gtaattagaa cagccaagca aaaacagctg ttcatgtttt tgaattggcg ggacttatta
     7261 ccgcagatgt aagcttttga ataccgttta gtggagaggt tgtgaaaaag ctgtttagtt
     7321 tcgagtatta agaaaaattt tgtggaaatg gctttccaca tttttgcata aatttggctt
     7381 aataccgaga aactggcttt tgaacaccgt ttaaggaaga ggttgtgcgc ctgccgttca
     7441 gctccgagtc acaaggagta atttcaaaaa acagtttttc atgtttgatg actattgtga
     7501 cttgtgaccg aggagttggc cggagagcac cgtttattct aaaaaagaac tgcgaactct
     7561 cgcagttctt tttttaaaat acgggataaa ggaagtttcc agcagacaag agaagaaagc
     7621 aaaagaaatt gctatttgat tcacaaggtt ctataacgaa atcgcaatca atcaaacatt
     7681 tacaaagaag gtaattcaaa tggaaatctg atttgatgat gcttctgctg ttgaaataaa
     7741 gcagcatcta gcacctgaaa acaaactaat agtaacattc gaagatggag taggtccgta
     7801 ttttcagcat gcaataatcc atatgtaagt tcaattcacc cttgaaatca tcggaccaga
     7861 taaagttatg accaatctat cccaagcaat ctagaagaga tattagtaaa gggacattta
     7921 ctagaagtct tagatgagcc tataaatata tgttctgatg gcgtcatcag tggtctgatt
     7981 gacggaagct taggtgtgat cgatctctct ggaccaaaca acgaaggaat caaggggaat
     8041 ccggcacgtt aagaaaaaaa tagcataaaa aactagacac ttgatgagcg ttcattcaat
     8101 tgtttgaagg gactaggctg gtgtcatttt ttatacatat attttctctt tcgtcttgat
     8161 agggcttatg ggataatata aggaagaaaa tcatcgtttt attcaaaaag gagtttttga
     8221 caaatgaaaa aacgtatatt atttatcggt gatagcatta cagacgcagg aagagaccga
     8281 gcagaagcaa ctgatctagg taaaggttat ccacttttga ttgccggaaa actaatctcc
     8341 caatatccaa cgatggaatt tgaatgcatg aaccgtggga taggaggaga caaaattgca
     8401 gatctgcaag tacgctggca agaagattgt ttagctttac aacctgacta tgtatcgatt
     8461 ttgatcggaa tcaatgatac ctggcataat actagcgatc aggctatatt cggaactgag
     8521 aaatctgctc agcaatttga atccgtttat cgaaaattac taaatgaact tcaaacagtg
     8581 ggaatctgca acatcatctt gatggagccg tttgtacttc ctataaccga cgaccgtcat
     8641 ggctggcgta aagacttaga ccctaaaatc caaattgttc gtaaacttgc taaggaatat
     8701 ggcgcattgc tggttccttt agatggtata ttcaatgctt tagggattgt ttatggtttt
     8761 tcgaaatata caagagaaga tggggtccac ccaacgattg gcggacatga gatcattgca
     8821 caaagctgga tagatgcaat tgccgcagtc gattttttta gtggagataa aaatgagcga
     8881 aagaaaagtg agaaatgatg gaaatacggt gggcagtaaa agaagatcta cctcaaatca
     8941 atcagctgat tactgatcat ccagaatcat tcgtaaaagc aaaaacggca gctgacctaa
     9001 atgataagaa taaactattg ctcgttgctg taacaaaaga aaaacggatt atcggatttg
     9061 cgcaagcgga cgtgcaacag tcagaagaac gattggatca gcgaccatca ggaaaactaa
     9121 tccaacacac attagtaaca aaagaaaata cggaagaaat ccaaaaaaaa tttgcagaat
     9181 atttgatact ggaagctaaa aaaagaagaa taaaatcatt ttttgcccag ttttaatcaa
     9241 aaacagtaat agaaggagaa atacaatgag ttgtttagga aatgtcatct ggtttatatt
     9301 cggcggacta atcggtggtc ttagctggtt agcagcgggg atactttggt gtatcacgat
     9361 cataggtatc ccaatcggta tgcaatgttt taagatcgcc tccctttctt ttgcgccttt
     9421 tggcaaagaa gtaatttatc accagtctgg catgtcattg attgttaata tcatctggct
     9481 gatcgtttca ggcctaccaa ttgctctcgc acatgtcgtc agcggcatcc ttctatgtat
     9541 cacgatcata gggatacctt ttgccaagca atctttcaaa cttgctcgat tggcgttgat
     9601 gccattcggt gcagaaattg tataaaagag aataataaag gttagataaa gcatagagtc
     9661 aggtgggata catgtatttc acctgactct atgcgttctc ctgtataagt tcttccatgt
     9721 gacttttacg tcttttattt atatggggtt actgtttatg gaaagttgaa ataattttca
     9781 ttaaagaact ctttctatat ttttgaaagc gtagtggagc ttcgaatatc tgggaaggac
     9841 attttgcagg gattgaaaaa agatattgtc ctatcgaaaa attaatgaat ttagacaaat
     9901 tgccagcaac aggcttcaaa cttagttgct atcctgtcaa gatcaagaag gcaagtgcgg
     9961 gttggataag agcagtggca atgatcgaaa agaaaaagaa agaataagcc aaaacatctg
    10021 tatggaagaa attttccgat gcagatgttt ttcatttttt ctttcggata ccttgacata
    10081 tgaataaccg ttcatttata attagttatg aagagatatt catatgttga ggcgaggagg
    10141 tcattaaaat gaaaagttac agattagaag gtttagattg tgcgaactgt gcaatgaaaa
    10201 tcgaaaaagg agttcagaag atcaatggag tcaaggaagc aacggtaaac ttcacttctg
    10261 gaaaactaac gattgacgca gaggaagatc atttggctac gattgaacaa gaaataaaaa
    10321 aagtagtgaa ggaattagaa ccagatgtca aagtaacaga aatagataag gaaaaagtat
    10381 ctgaacatgg aaatgaaaaa gagagaaata cgctgttccg gattttattt tcccttgcag
    10441 gtatcgcttt attgctatta tttgatttta atgaaccaat tcgcttgata gggtatttat
    10501 tgatttatct gctgatagga tatgatgtag tgaagaaggc agtgatgaat atcgtcaaag
    10561 gaaaaatctt tgatgagaat tttcttatgt ctgttgctac gattggcgct atgttgatcg
    10621 gtgaatatcc agaagccgta gcagtgatgc tattttatca aatcggcgag tatttccaag
    10681 gattggcagt cagtcattca agaaaatcga ttcgcgaatt gatggcgatc cgtccagaaa
    10741 cagctcacgt gcagacgacg gaaggattga tgaccgtgaa ccctgaagac gtgcagatcg
    10801 gtcagtttgt acttgtgaag cctggtgaac gtgtaccgtt agatggaacg atcatcgaag
    10861 gagaatcatt agtcgatacg tcagcactta caggagaatc agtccctaaa tctgtatatg
    10921 ttggtgaaac agtattaagt ggatttatca ataaaaataa accattttta gttcaagtag
    10981 aaaaatctta tgaaaattct actatcagca aattattaga attagtagaa aatgccagca
    11041 gtaaaaaagc accagctgaa aatttcatta ctaaattcgc gcgctattac acaccagtcg
    11101 ttgtaggatt ggccgtttta ttagctgttt tacctccact tgttgtttct ggagcggcgt
    11161 tttctgaatg gatctatcga gcattgacat ttttagtgat ctcatgtcca tgtgcattag
    11221 tgatctccgt accccttagt ttctttggcg gtatcggagg agcaagtaaa atcggtgtat
    11281 tagtaaaagg ttcaaattac ttggagttgc ttgcacaaac tgagacagtg gtattcgata
    11341 aaaccggcac attaacgaaa ggtgactttt cgatccagac gatcgataca aaaatcgatc
    11401 caaagatatt catgcaatat gttgcgagtg cagaacagtt ttctacacat ccgattgctc
    11461 aatctgtttt agaaggctac gcaggtccgc ttttaccaac agctaatatc caagaatttg
    11521 caggtgaagg gatcttagct gaagttgatg gcaaacaagt attagcaggt aatcacaaat
    11581 tgatggaaag atttgaaatc tcttttcctt caagtcaaga aatcggtaca ttgctttatc
    11641 tagcgatcga tcaaagttac agtggttatc ttgttattgc agataccttg aaagaagatg
    11701 ccgtagacgc attggttcaa ttaaaaaagg ctggtgtgaa gaacacagtc atgttgacgg
    11761 gcgattcgaa aaaaattgct gatcatatcg ggaagcaagt aggcgtggac aagatatata
    11821 gcgaattgct tccagaagat aaagtgcagc ggttagagga aatcttacaa agaaacaata
    11881 aaaagaccgc atttgtcgga gatggaatca acgatgcacc ggtgctagca agagcagatg
    11941 taggaatcgc aatgggtgga ttgggaagcg atgctgcaat tgaagcagca gatgtagtca
    12001 tcatgaatga tcaaccatcg aagattgcag aagcaatcca tttagcaaag aaaacattga
    12061 aaatcgtcaa acagaacatt gtctttgcta ttgggataaa aattttggta cttgccttag
    12121 gtgcttttgg ttttgcatca atgggagatg cagtatttgc agatgttggg gtgacagtat
    12181 tagctgtttt gaatgccatg cgaagcttgc atgtaaaaaa taaacgttga tcaatgaaga
    12241 ggacgaatga acgtcctctt ttttttatat ccttttctta atg
//
LOCUS       DACECZ010000080        12204 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.080, whole genome shotgun sequence.
ACCESSION   DACECZ010000080 DACECZ010000000
VERSION     DACECZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.080"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            625..1953
                     /locus_tag="IWO35_001959"
     CDS             625..1953
                     /locus_tag="IWO35_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0317016.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2155..2988
                     /locus_tag="IWO35_001960"
     CDS             2155..2988
                     /locus_tag="IWO35_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ0317017.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3004..3741
                     /locus_tag="IWO35_001961"
     CDS             3004..3741
                     /locus_tag="IWO35_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ0317018.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3896..4864)
                     /locus_tag="IWO35_001962"
     CDS             complement(3896..4864)
                     /locus_tag="IWO35_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ0317019.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELKKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5096..5929
                     /locus_tag="IWO35_001963"
     CDS             5096..5929
                     /locus_tag="IWO35_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ0317020.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5963..6802
                     /locus_tag="IWO35_001964"
     CDS             5963..6802
                     /locus_tag="IWO35_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0317021.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6828..7388
                     /locus_tag="IWO35_001965"
     CDS             6828..7388
                     /locus_tag="IWO35_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0317022.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7587..9308
                     /locus_tag="IWO35_001966"
     CDS             7587..9308
                     /locus_tag="IWO35_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ0317023.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9473..10615
                     /locus_tag="IWO35_001967"
     CDS             9473..10615
                     /locus_tag="IWO35_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0317024.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10631..11842
                     /gene="thiI"
                     /locus_tag="IWO35_001968"
     CDS             10631..11842
                     /gene="thiI"
                     /locus_tag="IWO35_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ0317025.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4155 a         2048 c         2537 g         3464 t
ORIGIN      
        1 gtcgtaggtt cgagtcctac tgccggagta aataaagctt atgcaaagaa aaagaaatcc
       61 aatcacttgg gtttcttttt cttttattat tatgatgaat caagctttct gtttttgtca
      121 cgtatgttgt aatctttacg aaagagtaga gttggctact cataatcaac catacttccc
      181 atcatcgcta aatacgaaat aaatcagcat tcctacacca atcaaagcac aagctgcacc
      241 aagaaaaggg atgacattcc caaagttatc tgtattcata agtatgccac cacccacaaa
      301 caaaaagccg ccagcaatca ggtttttcat tttattcatc tcctttctta cgttatagta
      361 tttccttctt tcaatgagta ttttgaaagc tatcttattg tatgatgttc ttctaggcct
      421 cttctttatt atacaggcca ttttcgttat ctcatagaaa tattcctttt tatctgaaaa
      481 ctattgtata ccgtatgaca tttttcttca tgaagttgta agtatattct cattcaaatg
      541 aaaatttcat catagaagag attgtatttc taaattctta agagtaaaat acgaagtata
      601 acttggattt ggaaggatga ttggatgaaa gctattacgc ctgatatgac aacaaaattt
      661 aacaaacagt ttcaagcaaa tatcaaacag caggcattgc agcgggcagt tgtgaagaac
      721 ggaattactg cttctgctga aaatgtttca gcacatgtgg agaatacacc ggtattttct
      781 atagatttgg ctacggggaa agtggctaat caaatgcaat cggggcgttg ctggatgttt
      841 gctgcactga acacattccg tcataagatg ctgaatcaat tccatttaaa agagtttgaa
      901 ttatcacaaa attacacttt tttctgggat aaatatgaaa aggcaaatta tttctatgaa
      961 aacatcattg caacaggaaa agaggcaatc gatagccgga aagtagcttt cttactcgct
     1021 actcctcaac aagatggcgg ccaatgggat atgatcgttt cattattcca aaaatatggc
     1081 gttgtgccta aatcggtgat gccggaaagt gcgaatagtt caaattctcg agatttgaat
     1141 aactacttga acaaattact gcgtaaagat gcagtagtgt tacggaaaat ggttgctcaa
     1201 ggagaaacat tagacgctat tgaagaaaag aaagaagaaa tgctagaaag catttataac
     1261 ttcttagcta tctcattagg aacacctcca aaagaattcg attttgagta ccgcgatgaa
     1321 gaaaagaact atcatcttga ccgaggactg acaccacaaa ttttttatga taaatacatc
     1381 ggtgtaaaat tagatgatta tgttagcgtg atcaatgcac caacaaaaga caaaccattc
     1441 aatcgttctt atacagtaga gatgctagga aatgtcgttg gcgggaaaga agtcaaatat
     1501 ctgaatgttg atatggaaac tttcaaaaaa ttagcgattg ctcagcttga agaaggagaa
     1561 tctgtttggt ttggttgtga tgtaggtcaa tcttctacta gaactagtgg catcatggcc
     1621 ttagatgctt attcaatgga tgatctattt gatacggatt ttacaatgac taaagctgaa
     1681 cgtttagatt ttggagaaag tctgatgacc catgcgatgg tattgaccgg tgttgactta
     1741 gtagacgggc aatcaacaaa atggaaagtc gaaaatagct ggggcgaaaa agttggaaag
     1801 aatggcttct ttgttatgag tgatgcctgg atggacgaat atacgtacca aatcgttgta
     1861 agaaaagatt tattgacgaa agagcaatta aatgcgttca atgaagaacc aattgttcta
     1921 tctccgtggg atccaatggg tgctttagca tagacgagta tttcaatgaa gagtatgtag
     1981 taaagataaa aagagctggc ttaagccagc tctttttatc tttaaagtcc attccctgtt
     2041 ttttatggtc cctgtgctat aattctaaat aaaggataag gattggaaat aatcctttta
     2101 tcattaaaaa tatgatgacg aagtagaata aaattgggag taggggggaa agtcatggat
     2161 atccaaactg ttttatctga tctgaaatta aatggtaaag tgattccagt tgttggtggc
     2221 gatgttaatc agacctatcg tataaaaacc gaacatcgtg cttatttttt aaaaatccat
     2281 ccgaatgtaa aaaaaggatt ttttgaagca gaagtagacg gattaaaaga actgtcagca
     2341 ttcgtacgtg taccggatac gtatatgcta ggtgaaacaa gtgaaggcgc ttatttgtta
     2401 atggaatgga tcgagccggg aaaaggggat caacgagatc ttgctgcagc tttagctaat
     2461 ctccatcagc aaacagcgcc acaatttggt tttcgtaaag ataactattt gggtacactt
     2521 gttcagaaaa attcttttga agaagattgg tggacttttt tctttaaaga tcggcttgaa
     2581 tcccagattt ctttggcaga agaaacgaat cgatggaatg tacagcgtca agaaaagtat
     2641 ctgcgattta aagaacgtgt attgaaatcg gttgaaccaa aaaagatcac tccacgcctc
     2701 ttgcacggag acctgtggag tggtaatgtg ttctttgacc agcaagggca tccagttttt
     2761 gtcgacccag ctgtttctta tgggaatcgg gaacaagata ttgcaatgag ccagttattc
     2821 ggtggtttcc gaccggaatt tttagatgct tatcaaacta tatttccatt agaaaaggga
     2881 tggaaagacc gactgccgat ttaccagtta tattatcttt tggcacattt gaatatgttt
     2941 ggagaaagtt acggttccca agtagatcaa ttactagaaa atttttaaga ggagggcgtg
     3001 tttttggcaa aaaaatatac caaaaagcat gaagtttcat attatgagtg tgacatcaac
     3061 caaacgatga catttccttc gatgttagga gtagttatca aaacgtctga agatcaaagc
     3121 gaccagttgg gaagaggcag tgaattcgtc aatagctttg gacttacttg ggtcatcacg
     3181 aattattcca tggagattaa tcgtctccct agagtcggtg aagtaatctc agtaacgact
     3241 caagccatgg agtacaataa gtttttctgt tatcggaact tctggatcca tgatgaagat
     3301 gggaaagaat tagtcaaaat agaatctgtt ttcgtattga tggattttgt gaatcgaaaa
     3361 atgagcagtg ttaatgaaga gatcattgct ccttttgaga gtgagaagat caagaaaatc
     3421 aagcgtcagg aaaaaattga aaagattgat tcgggcttca tgttgccata tcgtgttcgt
     3481 ttttatgata tcgatagtaa tcagcatgtt aataatgcga tgtattttaa ttggatcatt
     3541 gacgtattag gatatgactt tttgactacg catgttccag aaaaagtaat tattcgattt
     3601 gataaagaag tagagtacgg gaatgagatc gaaagccatt ttgaacaaat ccctatcgaa
     3661 aatggtgtca aaacaaaaca tgaaatccga atgcaagaac agctgtattg tgaagcaaat
     3721 attatatgga aaaataaata aaaaagaggt tgtgacagaa gggggcaact tcaagaaata
     3781 agaagaaatt tacgaaaatt gtttctcaaa tttttgtaaa ttttcgactt agttttcgaa
     3841 gaagctattt ttgtcacagc ctctcttttt aagtttagct attagataat gatgtttagt
     3901 tttggataaa cgaaatcaat tcttcacccg acaaaccata gtttacagca atcaatagtt
     3961 ttaaacgtgc tttttgacta ttaatactgt tacagaaaat aatccccatt ttttttagtt
     4021 cttttccccc gccttcataa tcataaacag gctctgcgat tccattgaaa caacgagaaa
     4081 caagtactac agggattttt tggacaagaa gcttttccaa agcagctaag gcttgtggag
     4141 ggagattccc cgcacctaaa gcttcgatta ccaaaccgtc tactttcgta tgggcgatgg
     4201 cttccaataa gtccccttgc atcccagcat atgctttaac gatcgggata gtcccatcta
     4261 cagcggaaat gtccagtcgc tttgtttcta aaagttcctg caagaataaa attcgattct
     4321 tagttactaa gccgatggga cccaatgtag gtgttcggaa agtagcaaca tttgtggtat
     4381 gggtctttgt cacgtagcgt gcagaatgga tctcatcgtt catgaccacc aaaaccccct
     4441 tatctagcgc ttcttcacaa cttgcgacac gaatcgcact ttcaaaatta taaaggccgt
     4501 cacttccaag ttcattactt gaacgcattg ccccagtcaa aacaattggt aatttgttgc
     4561 caatcgttgt atcaagaaaa aaagcagttt cttctaatgt atctgtaccg tgtgtgatca
     4621 ccactccgga tatacctgat gcataagcgg acaagatacg ttcttttaat accaacatgt
     4681 gagaaggcat gatgtgtggt gagggaagat tgaagatatc ttcaactaat aattctacat
     4741 gcttggggat tgtgatttct gcatctaata atgggtttgc cacatcagga ctaacaccgc
     4801 ccgtgagcat gttttctttc atggcgatcg ttccgccggt gtgtagtaca agtatttttt
     4861 tcattttaca gacttcctta cgaataattt tgcatagccg ttaaatctta ttgtatacta
     4921 tataaaagag ttttgctatc agtgaagaat aacgaatagc ctaacttatc ataaaaaagc
     4981 tgaccttatt aaaatggaaa agactcaacc ttgacctcct aatacaatgg agtatctggt
     5041 tgagtttctt ttacaagaaa gcagccaagg aaatcaatca cgaaggtggc cggaaatgat
     5101 cagaatcgga ttaacatcgt ttaaagaaca tgacaaatta actggtaaaa agaataacac
     5161 tctttatgaa tatgcagggt ttctaccctt agtggaatta gatactgcct attatggaat
     5221 tccccgaaag tcctcagttg agaattggat aagtgaagta cctgaatcat ttcgctttgt
     5281 agtcaaagtc tatcaaggaa tcagcggtca aggagactgg aagccttatt atcagaccga
     5341 agaagagatg attgcgcatt ttttagatgt tatgtcgcca atggtagatg ctggaaagat
     5401 gttttgcttt ttagtccaat tcccctcatc attcaaatgt acgaaagaaa atgtccagta
     5461 cttgcgcaaa cttagaaagt ggtttgaggg ttatcctgtt tctatagaat tgcgggatta
     5521 ttcttggtac agcgatgaat ttcttgaacg tacaagaaac tttatgtcta gagccgcttt
     5581 ttctttagcc atcatcgatg agccacagtt gctgaataca acgattcctt ttgatccatt
     5641 tgtcaccaat ccgaactttg ctctttttcg attccatggt aggaatgcgc aaggatggca
     5701 agcaaatgat aaagagtggc gtaaaaaaag aacgttatat cgttattcag aaaaagaact
     5761 gcaagaacta gcacatgaag tcaaagtagt tgattctaag acaaaagagg taggtgtgat
     5821 ttttaataat aattctggtg gtgatgcggc tgaaaatgca ttagcactgc aaaaaatatt
     5881 aaatatcaag ccagatggac tcaatccaac ccaaatggat ctgttttaag ggcttatttt
     5941 attcaagaaa ggggcgaagt ctatgatcaa agctattttt tttgatattg acggaacatt
     6001 ggtcaccaat cgttcaaaag cgttggaatc aacaaaacaa gcaattgaat atgctcggaa
     6061 aaacggcatt ttatgcggtg tagcaacggg gcgcagccca gtaaagatca aagaaatcat
     6121 tgatgaattg gaattagata tgtatgtcgt gtataatggt caactggttt ttactgcaga
     6181 tcgcacgatt atcgaccacc cttttgaaca aaaagtattg gaacatattg ttgaatttgc
     6241 agatgagaac catcgacaaa tcgtttttgg tgcgagaaat cggttagatg gatcaacgac
     6301 gatgctatta ggacaatcga tttttatcaa gcgactagtc agctttttac cgcgtaaatt
     6361 cccggtacgt ctgatgaaaa aaatcctcca ggtttttagc cctcaccgac aaaaggatcg
     6421 ttatgaaacg ttagagatat taaaagaacc aatttatcaa tgtatattgc tcagtccgga
     6481 gtcagaacag aaaaagttag aacatcgctt tcccgagtgt acgtttcagc gttcaaatac
     6541 gtatactgta gatatcatcc caaaaggtgg ttcaaaatta ctaggcatcc aagctttcgc
     6601 aaatgctgtc gaaattgaaa tggaagaaat tatggcattc ggcgatcatt acaatgatat
     6661 cgagatgctg aaaggtgtag gaattggagt ggccatgggg aatgctcaga tcgaagtaaa
     6721 acatgctgca gactttgtga ctcaatcaaa tgaagaagat gggatatacg aaggactgaa
     6781 acacttcggt ctgatttatt agtcaatgaa agaaggaaaa aatcagtatg gaagcagaga
     6841 caccttttca aaaagtagaa acattccaca aaacttttga tccaagaaga ccagagatac
     6901 ccacggcttt tacagaaaat gaagctcata cacgtgctgc gttcaaaata gaagaacttg
     6961 ttgaatttgt tgcagctact tcacctaata aagaacaact gcatgaagct gttatgctgc
     7021 tgcacgaagc attagataaa gcagaagaaa aaatacttgg taaaacacag tggggaagtg
     7081 atccgctggt tgaacaagta gatgcactga cagatatcct ttactttact tatggttctt
     7141 tttcattact aggaatagtg cctgatcgaa tattcgaaat cgtccatcag gcaaatatgg
     7201 ggaaattatt tccagatgga aaacctcatt atgaccctgt aacaaataaa gtgttgaaac
     7261 ctgaaaactg gcagatggaa tatgctccag aacctaaaat cgcggcagaa atccaacgtc
     7321 agattcaaga agcgattgct aagaagaaat ccgctgatat aggcatgtgc acaaatgaaa
     7381 aagagtgatc agagtttcac tatttttgtt atctttatac gatggaaaaa caaaagattt
     7441 tcgtttaagt tagtgaatgc atgaaaatag tatgctataa tctagaatag atgtcttttg
     7501 cacgagagac gtattatttg gatttatgcc aagcagaacg ccatctgttg cataaaagat
     7561 gcatactgaa aacggaggaa ccataaatgg gaaataatgt tatcatcgga atcgtcattg
     7621 caatcattat agtcgccatt gtcgtctttt tggttagtta cttgatgaag aaaaaaaacc
     7681 aagatcgatt ggatgcctta gaaaaacgca aggaagaatt atttgacttg ccagtgattg
     7741 aagaagtcga tgatgttaaa aaaatgcatc tggttgggca aagccaaaat acctttcgtg
     7801 aatggagtca aaaatggaat gacatatcta cttcctcgtt cgctgaacta gaaagtcaaa
     7861 ttttcgaagt ggaaaacctc aatgaaagtt ttcggttctt aaaaggaaaa aaagcagttg
     7921 ttgaagcaga agctacaatg gaccaaatgg agaaacaagt gacggaaatc cgtgaaggtc
     7981 taaaggaatt gcgggaaacg gaagaacgca actcactagc tgttcagcag gcacttgacg
     8041 tctatgaaga actgaaagaa aaagtcagag aagacgagca tgaatacgga ccagctcttt
     8101 ttgaattgca aaaacagatc aaagtgatcg aaaaagaatt tacgcagttt gtgactttaa
     8161 acacttctgg agaccctgtt gaagcccgcg aggttttgga taaagcagaa aaacatacgt
     8221 atgaagtgga agctctgatg aaaaaaattc caccgctgta tgaggagctc cataccactt
     8281 tccctgaaca acttgaagaa atcagtgata cttacgagaa attgatggaa gaacaatatg
     8341 tattcccaga agaagatctt gctgaagata tcgcaaaagt aagtcgaaga attgaaaatt
     8401 cattagccaa tcttgaaaaa acagaagttg aaacggtcga atttgaaaac cgtgaaacag
     8461 ctgatttgat tgattcgtta tatgatatct tagaacggga aatggaagct cagagatatg
     8521 taaaaactaa ccaatcaacg attgcagagt atatcaaaca tacaacaaag aataaccgtc
     8581 aattattgat tgagctggat cacacatctc aaagctatac attgaaccac aatgaaattg
     8641 gccgtgtacg aggtttccaa accgaagtgg aagaaatgga acgtcaaaat gaacagatga
     8701 ttccacagat ccgtcaacac gaaattcctt attcagaagt gcgcacgttc tataaaacag
     8761 tcttcaaagt cttagaagat atcgaaaagc aacaagtaga gatcgacgac tcattacatg
     8821 aattgagaaa aggcgaaaaa gaagcgcaag aaaagatcga tacattcgaa ttcaaattgc
     8881 gcagtttgaa acgttttgta gaaaaacaac gtttgcctgg cttgccgaat gattacctag
     8941 aattcttttt tgttgctact gaccgtattg aagaactaag tgttgtcttg aataaaattc
     9001 gagtaaacat ggaagaggtc aatcgtttag ttgctctatg tgaagaagat ctagaattat
     9061 tagataagaa gacacatgat ttagtagatg cagcagcatt aacggagcaa atgctccaat
     9121 atgcaaatcg ttatcgtcat acacacgaag aagtgagaga agcaattgat aaaagctact
     9181 atttattcaa taaagaatat cactatcaag aagcattaga cgaaatcggt acagcattag
     9241 agcgagtaga acctggtgct ttcaaacgca tcgaagattt ttattttaat catccagact
     9301 tagtataaag caaaaaagtt agtaaaccag caagagcaag acggattatt gtcttgttct
     9361 tgtctttttt tgtatatttg aaaattgaaa caagtgaaaa tttcacctgc tcttgaaagc
     9421 accttggaca accgttataa tagaaaagaa tttcaaaaaa gaggggtttc ttatgatata
     9481 ttttgataac agtgcaacaa ccccaatctt tccgcaaagc ttagatgcat atgtaaaaac
     9541 cagtcagcgg attattggga atccatcaag cttgcatgat ttaggcaatc aagctaaccg
     9601 gttgttgcag caagcaagaa agcagatcgc tgatttgatc catgtagaac cagaagaaat
     9661 ctattttact tcaggcggaa cagaaggaga taactgggta ttaaaaggaa cgatgatcga
     9721 aaaaagagaa tatgggaatc atatgatcat ttcaggagtc gaacatccgg ccgtcagcga
     9781 gacagcagaa caattaaaag aattaggaat cgaagtaagt atcgccccag tggataaacg
     9841 cggatttgta cgtgtcgacg aattgaaaga attaataaga aaagagacag tattagtctc
     9901 tgttatggct gtgaataatg aagtaggatc gattcagccc atccaggaaa tcagtgatct
     9961 tttggatgat tttcctaaaa ttcatttcca tgtagatgct gttcaggcga ttggaaaagt
    10021 accattctcg caatggttga cagaaagagt tgattttgca acattttcag ctcataaatt
    10081 ccatggccca cggggaactg gtttcattta ttggaaaaag ggtcgtcgtt tagcaccatt
    10141 actgacaggt ggagggcaag aaagaaatca gcgaagtggg acagaaaatg ttccaggtat
    10201 tgtggctatg gttaaagctt tacgtttatc attagagata aaggaacaaa aacctgagca
    10261 gacacaacgc ttaaaagtag ctctaatcaa tgcgttgaag acgtatgaga aagtgacgat
    10321 tttttcagaa ggaactgact atgcaccgca tattttatgt tttgctctaa aaggaattcg
    10381 cggcgaagtt ctcgtccatg ccttagaaga aaaacaaata tttgtttcta ctactagtgc
    10441 ctgctcctca cgtaaacata tggcaagcag tacattgcac gcaatgcatg taccaaatga
    10501 tctggctaca tctgctgttc gaatcagctt agatgaaaac aattcgcttg cagaagtcga
    10561 gcaatttatg attattttca agcagcttta taagaaattt tctgcaatca attaaaagag
    10621 gagtgttctt gtgaaataca gtgaaattat ggtccgttat ggggaacttt cgacaaaagg
    10681 aaagaatcga aaaagcttta tcatgcaact agcacaaaat gtcagacaag ctttgaaaga
    10741 ctttccagaa atcaaaatcc atgctgatcg ggaccggatg catcttttat tgaatggcgc
    10801 agacgggcag ctagttattc caaaactagc aaaaatattt gggatccaaa atttctctcc
    10861 aagtattcgt gtagaaaaag atgtcgaagt tttgaaacaa gctgttcaag acatcatgaa
    10921 agaaatttat actgggaaag aaacatttaa aattatcgct aaaagaagcg atcaccaatt
    10981 tgaattgaca agtaatgaat tgaatcaaac attaggtaat gctgtttttg atattttccc
    11041 gcatatccaa gttcaaatgc gtcaaccaga tattccatta cgtgtagaga ttcggcgtga
    11101 tggcgcttat ctatcgtatg aaacgatcaa gggagctgga ggattgcccg taggaacaag
    11161 cggaaaagga atgcttatgc tttctggggg gattgattct cctgtagcag ggtactttgc
    11221 catgaagcgg ggagtggaaa ttgaagctgt tcattttgct agtccgccgt atacaagtga
    11281 acaagcactg caaaaagcaa aagatctaac agccaaacta gcaccttatg tgggaacgat
    11341 ccattttatc gaagtgccat ttaccgagat tcaagaagaa atcaaaaagt cagtgccaca
    11401 aggatattgg atgacaatta ctcgtcgaat gatgttgcgt ttgaccgatg aaattcgccg
    11461 tattcgtcat ggtttagtca ttttgaatgg tgaatcatta ggtcaagtgg cttcacaaac
    11521 tttgcaaagt atggtggcta tcaatgaagt aacgaatacg ccaatcattc ggcctgtagc
    11581 gacaatggat aaacttgaaa tcattgaagt agcagaacag atcgatacat ttaatctttc
    11641 tatccagcct tttgaagatt gctgtacgat ttttgctcct ccacagccaa aaacacgccc
    11701 tcaactggaa aaggtccatc aatatgaaga gcgtttagca atagatgaaa tgatccaacg
    11761 agcactagct ggcttgaaga tggaagagat acaagcagaa tcgtctaatg aaaaaactga
    11821 agaattttca gaattgctat aagaggttat gaaaatagtg ttttgaccag agtattagag
    11881 aaacaaaacg aaaaaatagc tccatgtatt ttaccgtttt tgaactaatg tcgaagatta
    11941 gctgattgaa taccatttat caagaagtta tggaaaagcc ggttcttttt tactaactga
    12001 aaaataaaat tttatttaga gatcaagcta cagttgataa atgatataaa aaagagaatt
    12061 tcccatgaaa tagcgaggtt gtgacagaag tgggcagctt caagaaataa gaaggaattt
    12121 acgaaaattg cttttcaaat ttttgtgaaa tttcggctta tttttcgaag aagttacttc
    12181 tggaacaccg tgtatcagag gttg
//
LOCUS       DACECZ010000081        11760 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.081, whole genome shotgun sequence.
ACCESSION   DACECZ010000081 DACECZ010000000
VERSION     DACECZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.081"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWO35_001969"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWO35_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0317026.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IWO35_001970"
     CDS             complement(322..891)
                     /locus_tag="IWO35_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ0317027.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IWO35_001971"
     CDS             1077..1979
                     /locus_tag="IWO35_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0317028.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWO35_001972"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWO35_001972"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ0317029.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWO35_001973"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWO35_001973"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ0317030.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWO35_001974"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWO35_001974"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ0317031.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IWO35_001975"
     CDS             8547..8732
                     /locus_tag="IWO35_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ0317032.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IWO35_001976"
     CDS             complement(8794..9990)
                     /locus_tag="IWO35_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0317033.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IWO35_001977"
     CDS             complement(10160..11596)
                     /locus_tag="IWO35_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0317034.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
BASE COUNT         3482 a         2501 c         2058 g         3719 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
//
LOCUS       DACECZ010000082        11738 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.082, whole genome shotgun sequence.
ACCESSION   DACECZ010000082 DACECZ010000000
VERSION     DACECZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.082"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(68..994)
                     /gene="glsA"
                     /locus_tag="IWO35_001978"
     CDS             complement(68..994)
                     /gene="glsA"
                     /locus_tag="IWO35_001978"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ0317035.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1017..2228)
                     /locus_tag="IWO35_001979"
                     /pseudo
     CDS             complement(1017..2228)
                     /locus_tag="IWO35_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(2263..3876)
                     /locus_tag="IWO35_001980"
     CDS             complement(2263..3876)
                     /locus_tag="IWO35_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAQ0317036.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            complement(3988..4197)
                     /locus_tag="IWO35_001981"
     CDS             complement(3988..4197)
                     /locus_tag="IWO35_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAQ0317037.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            4288..4476
                     /locus_tag="IWO35_001982"
     CDS             4288..4476
                     /locus_tag="IWO35_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317038.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            complement(5186..6400)
                     /locus_tag="IWO35_001983"
     CDS             complement(5186..6400)
                     /locus_tag="IWO35_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0317039.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(6528..6998)
                     /locus_tag="IWO35_001984"
     CDS             complement(6528..6998)
                     /locus_tag="IWO35_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0317040.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            complement(6995..7237)
                     /locus_tag="IWO35_001985"
     CDS             complement(6995..7237)
                     /locus_tag="IWO35_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAQ0317041.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            complement(7783..8313)
                     /locus_tag="IWO35_001986"
     CDS             complement(7783..8313)
                     /locus_tag="IWO35_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0317042.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(8495..9151)
                     /locus_tag="IWO35_001987"
     CDS             complement(8495..9151)
                     /locus_tag="IWO35_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ0317043.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(9278..9751)
                     /gene="dfrF"
                     /locus_tag="IWO35_001988"
     CDS             complement(9278..9751)
                     /gene="dfrF"
                     /locus_tag="IWO35_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ0317044.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(10170..10388)
                     /locus_tag="IWO35_001989"
                     /pseudo
     CDS             complement(10170..10388)
                     /locus_tag="IWO35_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10419..11633)
                     /locus_tag="IWO35_001990"
     CDS             complement(10419..11633)
                     /locus_tag="IWO35_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ0317045.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         3431 a         2519 c         1827 g         3961 t
ORIGIN      
        1 aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca gcctctttca
       61 agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc aacaattgga
      121 taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct atgccgtaac
      181 gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt acaccaacat
      241 ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt gatttgacga
      301 ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt cctttatttg
      361 ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag cattgtctga
      421 aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct ttcatatagt
      481 aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt tcttcattca
      541 atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc tcaaattttt
      601 catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca ttaacaaaag
      661 gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg aaggcaaaac
      721 cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct ttgattgcta
      781 ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg gcgtctcctc
      841 cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt ttgggatcca
      901 cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag tggaaatttt
      961 ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt cattttttat
     1021 ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat aactaagacg
     1081 ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc tgcgatacta
     1141 gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc taaacaagca
     1201 acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag actgtcatct
     1261 ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag gtagattaca
     1321 acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct taatgtaaag
     1381 acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc aggaacaccg
     1441 tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc tacgtgaagt
     1501 gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa acaaacaaga
     1561 atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa taggaattct
     1621 aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt agtagcgacg
     1681 gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa cattgctaat
     1741 ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt ttctacgccc
     1801 atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga aaaatcagga
     1861 atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac ataatggatc
     1921 cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc gatccgagca
     1981 atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca gaaaataatc
     2041 aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc cgttgcatag
     2101 gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat ctcaaaccat
     2161 tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac ccaagtatag
     2221 acaccgccgg tttcccttag ttaaaaatat tactcttgtt gattactcaa ctcttccaat
     2281 aaatcggtaa tatatctcaa tttcctgtat tctttcgtta tcctcattcg ttgttgcttc
     2341 atgtatgagg attttttcta tcatggcatt taataatggt gctgtcagtt cctttggtac
     2401 ggaatattcc ttgattaact caatccactt ttcagcacct atcatatcct gttccatttt
     2461 acttagttct tctcttaggc ttgttatctg ctgttccagt tctatctgtt ctttttggta
     2521 cttgctggac aacatcacaa aatttcgctc tgttatcttc tcacaggctc tatcttcata
     2581 cattttcgca aataaacggt caatctcatt ttgtctgttc tcggctttct tcaatgtact
     2641 tgcctttttc ttcttttccc gtattctctc tgcattgcca gctttctgta tcttgttcaa
     2701 taccttttct tcgtcctgct gtactgcctt agcccaatac tgcaagcgtt ccaatacggc
     2761 ttgatacagc acatcataac gaatgtagtg catagaacaa ttacctttgc caaactgccc
     2821 gtagtaactg caagcataat aactgtaagg cgttttattt gtcttattcg ttccaaatct
     2881 catagaccaa ccacaatccg cacacttgac aagccctgca aaaatcggcg ttgccttttc
     2941 ctttgtctgt ctgcgtcttg attttatctg ctcctgcaca cgatagaaca cttccttgtc
     3001 aataatcggc tcgtgagtgt tttctactcg aaaccattca ctttcgggct tacgcacttt
     3061 ctttttgctc ttgaacgata ccgtagactg catattgtga acactgtttc cgatataaac
     3121 ctcacttttc aatatcgcct tgacatgagc aatcgtccac tcataacgct tactttcggg
     3181 ttttccctca aagatatgtg caaaagtacc gtatcttgta aaatttagcc atgacgctgt
     3241 cggaactttt tctgctctta actgctttgt gatttttgca cttccgtaac cttgataggc
     3301 tagggaaaag attttttcaa taatccattt tgtttcatca tcaaccaaca gttttccttt
     3361 gatgtcaggg tgtttcttat atcctaacgg agcataagca ccgtaatgag ccccctccgc
     3421 aaactttgtc ttaaatgctg atttgacttt acggcttgta tctcttgcca cccattcatt
     3481 gatgatgttc ttaaatggtg caatctcact ttcgcctttt tcgctgtcta caccgtcatc
     3541 aatcgctatg tagcggacat tatgactagg gaaatacaat tctgtatatt gtcctgtcat
     3601 aatataattt ctgccaagac gggaaaggtc tttcgttaca acacagttga tttttcctgc
     3661 ctctatatcc tcaatcattc tttgaaaact cggtctgtca aaatttgttc ccgaccagcc
     3721 gtcatcaata tattcatcaa tgacattcaa atgatgttct tttgcatata aacgcaacat
     3781 acttctctgt gtggtaatgc tggaactttc gccctgtaat tcatcatctc ggcttaacct
     3841 aagataaagt gcagtattgt aaatctgttg tttcattgtc agttatcctc ctttataact
     3901 aacccgtgtt tacaatacct gcttgcaagt aaagtataac acgggtactt tttgtcattc
     3961 aatctatttc ttacaactgc accgatttta tgcgattgtc tgctttgctt tttccagcat
     4021 gacatcagtt agaagttctt ccattgtttt gcccttttcg gaaaaatgct cttttactac
     4081 aatctttgtc tttcctctgt tgccaatgcc acacttgcca tttttctttt gaattgtttt
     4141 tacagcatta tcaataacac ataccccctt tctgtaaaat ctttctaagc aattcaacgg
     4201 cttttgtcat agcccacagg agttccacct ctgcatagtt cttatgtagc cgtacccatt
     4261 gcgtgcgacg ctcttaatgc tcaagctgtg gctgtacggg agtatcatta tctgacagta
     4321 caggtcatgg cgataaaaga ggacaaatct tttacaagaa cacaaatagg ttttcgctcg
     4381 ctcttgcagg ggaaaggtcg tggcgtattt gtttcaacgg ctagctgtct gtcaaacgta
     4441 ttgaatagct ttattcagtt gtcaaagaac gattgaaaga aaaaaatcgt ctttcctata
     4501 tatcgcgttg gaaaagagga gaaacgtaac caaaatccaa aactttttcc attattagta
     4561 caaatggaac ggttcatttt ggcaatcaac ggttgatttt ctcctttttc actactatct
     4621 gtacagcaaa cgtccatttg ctaagtttaa atggaaaaat tttaacttct tttcatgcac
     4681 catataggga tagaaaatgt cattttgcta agttgatgat gaaaaaagaa tattttcctt
     4741 ttcacaccct aactacccaa accataccgt tactgccaac aatagaatga aatattttct
     4801 ttgcaccata tagggaacgg aaaccttaaa ttgttaagtt gagaacaaag tattttcatc
     4861 ttttcacatc aacactcaac tgctaatgcc tgtttgaata caaaatgatg atgtttttcc
     4921 tcttttcaca gaagactgtc cactaatcac gaatttccgt cattttttca aaactttttt
     4981 cttctttcac ttcatatagg gacagcaagc cccgatttaa tgacctattt tcaaaaaaag
     5041 tttagatttc cttttctcgc accatatctg tacgcaagta gccattttgt tgcagattta
     5101 ataaaaaatt tatttttctt ctttcacttc acatagggaa tgaaaacagc tctttgctga
     5161 ctaaatctga aatttttcaa ttacattaaa aaataccgtc attctcttgt ggaacagcgg
     5221 tatctatacg ttcttctatg gttgatttct ctaattgtaa taaatgttta tgcttgtctt
     5281 ttagttctgc ctgctcaatc atatctttca gtatcgttgt acgattttct ctgtctaatg
     5341 cctgtaccat ttttatggtt ggtgcgactt gtctttgtag ccaatgcaac gctttttgta
     5401 aagtgtaagg ctctggcttt gtggttaagc gtatcggctc tctgttttcc cctacaaacc
     5461 atgcccaatc atcatttgtt atccattgac tttttggctt gtcgtcctct ctgtcaacaa
     5521 agcggacata atgattgatg atagaaaaag cggtgcgttc agcgtcccga taggtcagta
     5581 aatctacaac cgcataatag gctcgttcgt tcttcatgcg tatttcaaaa cggttcttaa
     5641 tctccgtatc ttcaatttct gtgccattct tgacatactg ctcataattt ttctgataag
     5701 cacacatata taattcgcta cttgttgaac cgagatataa ggtttctcct gtattctttg
     5761 gtaaatcgct accgcatttt tctgtaccgc tgtaatcttt ctgcatacgg aagtaggaca
     5821 tacattcgcc agccttgtat ttttccttta actttggaat atccaatatt cctgctttat
     5881 cattgatagc caaatcaagc cgtttcatca caccaccagc cgtcatacaa tccagcatga
     5941 agtcatacca tgagcgttcc tgtgccagca gataacattc catttgccga cagcctttgc
     6001 ctttcagttc caataaaaca cctaaatgct cctgcatgga acacatgata ttgatgtcgc
     6061 caagtacata tttgctttca tatccgtatt ttccataatc ttcataaagc atgtaattag
     6121 atttcagttg taatacatca cggataatcg ccaatgcgtc cgtagtagga aagcggacac
     6181 gaaagtaatc aatcaataag aatagtggtt cttgcggatt gaaacgctca atctgttttt
     6241 ctatgatttc tcttaattcg tcattcaatg gctgttttcc tttttcaatg cggttgtaat
     6301 attcacggct gataccacag gcaacagcaa gccttgtttg tgttactccg tattcctcac
     6361 gcttttgtct tagttcttca ataaagtttt tatcattcat tcttcaattc ctccaatcaa
     6421 aaaaggcaac taccataatt gataattgcc tgtcattaaa aatctgtata gaaaaagcag
     6481 tcaatcctaa gactaactgc tttgtgtgta tggatataaa attgcttcta atcttgaata
     6541 ggtatccaaa tttcaataac tgaattatca ctattccaat gaaaacgata atcgtatttt
     6601 tcaaagtgta ggataatatc ttttatagga atatacgatg tactaggaat aatttcttca
     6661 taaatcttgc cataagtttc atatatttta tccatagcac caatatgctc cacaaccaaa
     6721 tatttataag acggtatttc tttatacaga aagccctctg gggtaatggt tctttgggga
     6781 atagaacaga aataataaag ttttccattt cttctttcca taaaagcata ttttacccaa
     6841 tttcctgatt gtgaaagata cgcttttttc aaactactgt taaactttct ccaaaactgt
     6901 gtactaatct gaatattctt tttttgataa ttagtcagtt ctacttcctg cccaataact
     6961 gcaaatgcgt ttaattcctt tatagaaaaa tttatcattt taccttgtga gcagtgataa
     7021 cggtataact gtacgcattt accgttataa tacaattatc aatcgttata taccagtttt
     7081 ttccttttct tgtgatttcc gcattagaaa ggctaatttt ttctttgcac cattcaatta
     7141 cattttctac attcaaagat aagttctttt taattctaat aacgccaagt tcagttgtgt
     7201 gaagtcttcc taagttttcc agtaattcat tttccatatt ttgatacctc atttttcagc
     7261 aaatttattt gtatgtttta taccccaatt ataacattct catatcagca ctacaataaa
     7321 aataattaag caccaataat cttattgaac atctccaacc gtaaatcttt cacaccatca
     7381 gtgataaaca ctgagctgac agcaattacg acaactacca taattgcctg cacttaaatt
     7441 tctgcaaaag aaaacgacaa tcaatttctt attttttgaa atcaactgtc tttctcatat
     7501 ttactattca attttcatag tgatataggt acaatttata tcactactgt aaaaatgggg
     7561 ttttgcctta taaatacagc gttttccgct tgttagtgta tagtgtttct tgttttttgt
     7621 gccccccttg ttagataacg ggggctttga acggctcgca agctcgccga accgccgaag
     7681 cgaaccgccc ccttgcaagc atggcggaaa aatcttaagg attttcccgc caacgtggtc
     7741 ggatcgctcc gtcggtttaa cagcttttct gtttctctct agttatctac gcatataaat
     7801 caaatcggtt attccgttat aagtctgtgt tttacaaagc cttaacttta tttccctgtc
     7861 tatgttgccc aaaagacgaa ccccatttcc taacagagta ggaataacag aaatgtaata
     7921 tttatcaatc atatttttgc ttaccaactg ctgaacaaga tttgctccgc cacaaatcca
     7981 aatatccttt cctgcttctt gttttagccg ttccaataat aaaacaggat tttcacttgt
     8041 aaaatgaatt tgttgggaag aattgcactc tttatgtgtt aatacataag ttgtaaattt
     8101 attatatacc cattcttgcg gagaaagttc agtaacaacc tgatgagaag tattccaccc
     8161 cattaagaca gtatctatat ctttggtaaa ttctgtataa gtgtcgatgt tttcattgtc
     8221 atttccatga ccatttagcc aagctactcc accattaccg tccgcaatat atccgtcaag
     8281 gctcatggca ataaataaaa ctacttttct cattttttct cttttataaa tacaaacagg
     8341 aatttatttc tcttttttac tcataaggga acaactgatt gctacaaata aacccattaa
     8401 aatccatgga aatgctgctc ttataaaatc actaaccata ctttattcct ccttaaattc
     8461 caatttcctt agttctacaa actgaaattt ttgcctatga atgtatcttg ttctttatac
     8521 gcaaacaggc ttctgtgtgc ataatccaat gtcttgaaaa cggcatttga attagtccac
     8581 aaatatcttt attacaccaa taatcaatca gccaaattgc tttttcattg tcgtttacta
     8641 cattcaacga ttctaagtat ccttttcttt ctttcggtat ttttcttttc aattcatcat
     8701 aagataaccg ttcaagcatt ttctcagtgc tttcccatac ttcgaaaata tataaatata
     8761 attcttccag attaagctgt tttgaaaagt ctgcaatctg ctgtttcatg agttcatttc
     8821 ctgttgtaat gataggtgaa ttgatacgct cttgataatt gcctgcaaaa aatacttgtt
     8881 catcttcatt tatcactgta tgcacaacta tatcttcaat gcggaaaata tgccaaatag
     8941 aataagcaat cgtcttacta tgataaccat cagcgtttat gaaggggatt gagttaaaat
     9001 cttctctgca taattcttct ttgaaagata ctaaggtttg tattaactga cttcgtaaag
     9061 taagtaaagt atcaataccc ctcttataag tatctttctt ttttatttgt gcctgcattg
     9121 ttttattaag ttcagaccat tccttattca tattcaatcc tctctcaaat tctgatttta
     9181 tcgccccata caaactggaa tttgtcttac aacaatttat atatgcataa aaagcaatat
     9241 atttatgaaa acaccctaaa tccaaaattt actcaattca ttttttcctt acataaaatg
     9301 ttctcgtata tttcacttcc tcaccaagtg tttcccctat caaaacttca aaatcattga
     9361 tatcaaattc tggaaagaat gtatctccat cttcaatatt taaatctact tctgtgatat
     9421 acattttatc tactatttgc aaagcttcct taaatagtcc atatccacca gatatgtata
     9481 catctcgtcc tttagccaat aataatgcat cttctaatga tttaactgaa actaaattat
     9541 ctccttgata ctctgttgtg gtggaaacaa caatattcat tctattaggc aacggatgac
     9601 cgatttcttc ataagacttt cgccccataa taaccacatt acccgttgtt aactctctaa
     9661 attgcttttg ttctcccttt attttccatg gtatattacc attcttgcct ataacattat
     9721 tctttgacct cgcaacaatc aaacctatca tctatttcat cctccataat atgatgtcgt
     9781 acctgtgatt ccatcaagtg caccacatct taacaaactc aaaattatct cgaactaacc
     9841 agctcgacaa attccttttt tgtcttcatc tgcattatat catattgtta taagcattgc
     9901 catttctttt ctgcttttga aaagttactt atcagcctat gttttgtaaa gggtgatatt
     9961 cagcatttct cacgcaacaa gcccttgaca aaccatagac taataagttt taaggtaatc
    10021 aagcagaaat gaaatagtct gctgtttcac ggcaacaaaa aagagatacc aacacactta
    10081 tgtagtatct cttagtctag tagcaatatt cttttgtttg caagcaggta aacatgggct
    10141 gtttcttaat atctttttgt ttgggaaaca cctttttccc atcctttcac cgttgccatc
    10201 tctgctgcgc acaatgccac agggataaac catagcaaac cgcctaataa caagaaaaat
    10261 actaagctga agcctgaggt cgcgaatgtg ggatattcat ataatgaaac gaccattgag
    10321 atggtcatag ccaaaaagcc aaagaacgtt aatttttttg caggaatttt cgttttgctt
    10381 tgttgcatga atcttcctct tttctatttg aatttgtttc aattgagttt gttgccaaag
    10441 aagaaaaagt ccaatccttc ttcttgttct tcttttgtag tagtgatcgt tgtaaagtgc
    10501 atcgccgatt ttaaaatcac tgttgtcagc acataggcat acacggcagt aatgatgaca
    10561 ccaatcactt gaatccctaa gaagtaccag ctgcttggat tcgttagtaa atggctgtta
    10621 gatgcaaaga tccctatcag aatcgtacct gtgaatccgc ccatgccatg aacaccccac
    10681 acatcaagtg catcatccca ttgtttcttt ttacgaaaat caacagcagc atgacagacg
    10741 atacccgcaa tgatcccaat cagcatggca ctttttgctt ctacataacc ggcacaaggc
    10801 gtaatggctg ccaatccagc aacggatcct gttagtacgt cgacaaaatc aaagccattt
    10861 ccttttactt ttgcaatgat cagccaagta atcatcgcaa atgcaagagc gatgaatgtt
    10921 gaaccaaatg cattgactgc ttgttcatct gctcgaagtg caccaccgga attaaaaccg
    10981 aaccagccaa accaaagcag tcctgtaccg atagcagctg ccataagact gctatgctga
    11041 cttctttctc ctaaaggaat ctttctttga ccaagtgtga ggatacatgc taaagagccg
    11101 aatccagctg tcgtgtggat gactgctcca ccagcgaaat ctctaaatcc catctgatcc
    11161 aaaaatccgc caccccatat ccagtggcaa acagggaaat aaatcagcag attccaaaaa
    11221 ataacaagta taatatagcc ttttaaattc aatctccctg caaaagctcc tgtcatcaaa
    11281 ggaacggtaa tcacgataaa catcagttgg taaagaaaga acattaaaaa tggaatcgtt
    11341 gcaccatgaa acatgttggg gaagcttgta acatgtctca tcaaaaagaa atctgccggg
    11401 tttccaataa caccgccgat atcccgtcca aaggccaatc cgaatccacc aaatacccac
    11461 ataatcgttg ttatcccaac ggagagaaaa gattgagaca tgatcgttag tatattattc
    11521 ttccgtgcta atccgccgta aaaaaaagct agaccaggag tcatcaggca aaccattgct
    11581 gtacataaaa tcatgaatgc tgtatctcct gtattgatag gtaatgtatt catttttttc
    11641 gcctcctttc tacgattacg agtataaaag aaggtgtagt tgtttttttc ttctgttttt
    11701 ttgaattaat acttgcattt ataaaagaca gacaatta
//
LOCUS       DACECZ010000083        11551 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.083, whole genome shotgun sequence.
ACCESSION   DACECZ010000083 DACECZ010000000
VERSION     DACECZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.083"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..798)
                     /locus_tag="IWO35_001991"
     CDS             complement(<1..798)
                     /locus_tag="IWO35_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0317046.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQ"
     gene            complement(912..1496)
                     /locus_tag="IWO35_001992"
     CDS             complement(912..1496)
                     /locus_tag="IWO35_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAQ0317047.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(1623..2054)
                     /locus_tag="IWO35_001993"
     CDS             complement(1623..2054)
                     /locus_tag="IWO35_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0317048.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(2072..2392)
                     /locus_tag="IWO35_001994"
     CDS             complement(2072..2392)
                     /locus_tag="IWO35_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0317049.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            3129..3386
                     /locus_tag="IWO35_001995"
     CDS             3129..3386
                     /locus_tag="IWO35_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HAQ0317050.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(3357..3665)
                     /locus_tag="IWO35_001996"
     CDS             complement(3357..3665)
                     /locus_tag="IWO35_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317051.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            3743..4516
                     /locus_tag="IWO35_001997"
     CDS             3743..4516
                     /locus_tag="IWO35_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ0317052.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIRK
                     HGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSST
                     DSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPKA
                     DGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            4531..4734
                     /locus_tag="IWO35_001998"
     CDS             4531..4734
                     /locus_tag="IWO35_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0317053.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            4750..5070
                     /locus_tag="IWO35_001999"
     CDS             4750..5070
                     /locus_tag="IWO35_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ0317054.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRR"
     gene            5075..5254
                     /locus_tag="IWO35_002000"
     CDS             5075..5254
                     /locus_tag="IWO35_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317055.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(5297..5767)
                     /locus_tag="IWO35_002001"
     CDS             complement(5297..5767)
                     /locus_tag="IWO35_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317056.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            5854..6552
                     /locus_tag="IWO35_002002"
     CDS             5854..6552
                     /locus_tag="IWO35_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ0317057.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            6730..7071
                     /locus_tag="IWO35_002003"
     CDS             6730..7071
                     /locus_tag="IWO35_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317058.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            7064..7735
                     /locus_tag="IWO35_002004"
     CDS             7064..7735
                     /locus_tag="IWO35_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ0317059.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            7741..8427
                     /locus_tag="IWO35_002005"
     CDS             7741..8427
                     /locus_tag="IWO35_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317060.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            8430..9179
                     /locus_tag="IWO35_002006"
     CDS             8430..9179
                     /locus_tag="IWO35_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0317061.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            9191..9460
                     /locus_tag="IWO35_002007"
     CDS             9191..9460
                     /locus_tag="IWO35_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317062.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            9622..9924
                     /locus_tag="IWO35_002008"
     CDS             9622..9924
                     /locus_tag="IWO35_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317063.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            9921..10082
                     /locus_tag="IWO35_002009"
     CDS             9921..10082
                     /locus_tag="IWO35_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0317064.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            10079..10384
                     /locus_tag="IWO35_002010"
     CDS             10079..10384
                     /locus_tag="IWO35_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317065.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            10384..10740
                     /locus_tag="IWO35_002011"
     CDS             10384..10740
                     /locus_tag="IWO35_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ0317066.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            10700..10945
                     /locus_tag="IWO35_002012"
     CDS             10700..10945
                     /locus_tag="IWO35_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317067.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            10942..11355
                     /locus_tag="IWO35_002013"
     CDS             10942..11355
                     /locus_tag="IWO35_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002393044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317068.1"
                     /translation="MIPKFRAWVKPDILSNHLNGVIAEALPNFIYGNCLVLREDLEED
                     KQFEEIIDFLDIELMQSTGLKDKNGVEIFEGDIVKTLDADLKVTFSTIKFEEGAFCVD
                     YKDLGTEFDFLYFVDSPLEVIGNIYENSELLEEQR"
     gene            11352..>11551
                     /locus_tag="IWO35_002014"
     CDS             11352..>11551
                     /locus_tag="IWO35_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ0317069.1"
                     /translation="MNKQELIDKLSSLVGSIEDFKGISTFHAGKYAGLEHALELIQQL
                     NESQKPVIPQLVAGWIEKSTDN"
BASE COUNT         4125 a         1828 c         2231 g         3367 t
ORIGIN      
        1 ttgattgatt ttaaactttt ttaagagatc aatcgtatgt ttatcaatgg aaattttcct
       61 atttgaagtt tcgttttttg tttttcccca ttcatttaat ttataattcc atgttttatt
      121 aatagtgatg atttgatctt cgaaatcgat atcttcccat gttaatccta gaagttcagc
      181 aaatcgcatt ccagtagcac cagcaactaa cacgagcata ggagaagagt actgcgcaga
      241 aaggttttct tctacgagtt tcattaaaga tttaaattga tcataatcta aatatttatc
      301 ttcttttggc ttcaatgaat cttttccttt gattactgct tttctagttg gatcaaacgg
      361 aattaatcct tcttctacgg catctttcaa cgatgctcta atatggttat taaacttaat
      421 aacggtggat tttacatggt ctttcgcata cttatttaaa aagcgttgat atccaactct
      481 atctaaatca gaaatcaata ccgctggcat atatttcttt atgttcatta acgtatcttc
      541 gtattttcta taagtgatag gagatactgt tccttcttta taaagttgca tccaatcctc
      601 aaagtaatct gataataata gatttttgcg atccccttta aggcctttgt tcaagttata
      661 ttctaattcg ttggcggcat cttttgcttc gccttttgtc ttaaatcctg attttcttat
      721 cttcgtgtat tttccgtcat cttttttgta tgaaatttcg tattgccaac tatttccacg
      781 tttgactaat cttgccataa ttgaatctac tctctttctc tgatacaata ggcactataa
      841 agaagcctac tgtataggtt tgtttttctt agaacacgct tgctttggac ggtgggcgtg
      901 ttttttattt attatacagt tgctttaaaa atagctgctt tttcaaatga tactaaagga
      961 gagaagtgga tttctatttc tcccttttgg tttaacgtga aatgagcagt tacatccatt
     1021 tgttttcctg gagcaacaga tcccattgta ttttcgttgg catatgtctc tgattttttg
     1081 tcatctggtc catatacttc cacatctgta cctacaggga tatctgaatc gccatcattt
     1141 tttacggtat aagtaatttt tactacttgt gcaggttggt tttcttcaaa ttgatttctt
     1201 tcatcagtta gttctacact atttagtgta tattcagcat caccaacttt tacagtgtca
     1261 ccaatcttat agaaggtatc gcttttttct tctttagaag atgaagcaga ggttgaagat
     1321 ttagttactt tttcgccacc attatcactt gctttattac tatctgatcc accatttaaa
     1381 gcagaaccaa taattataat taaaattact gctaataccc aaaaccatac ttttttgtaa
     1441 aaaggtttac taactttata catttttccg tcttgaccca taactttttt tgccatttaa
     1501 atattcctca cttcttgtta taatatattt gcgatctcag aaatgaggta tgagtccgtg
     1561 tgccagcacg ggctttttta atgttttgct gaaatcggtt tttttaataa ttcttgatgt
     1621 attcatacat attgccttgc gtaagaatat tttttcttaa aatagcattg gcagacagca
     1681 taacaagtgt atctgagctt attctgaata gaataatact ccataaattt ttcgagatta
     1741 aactgagatt catcagtcag ttcgttctca atatagatat ttaataaaat tagaatagct
     1801 attttatctg cttctgtttc aaattttgag tgaaaagttg tagaagtatc gtacaaaact
     1861 gaaaattcaa aatgagaggc gctgaaatgt gcaagttcat gagataaatg aaaggcttct
     1921 gcagtttcac cgtatagatt ttcattcaaa aaaatgattc tgggttttgg atagtagaaa
     1981 ccaggctctt tcatctccat ataaactact ttcaaattgt attcgctcaa catttctttc
     2041 aatttcaaat acatacaaac catcactcca actattcatt ttcctctaaa gctttagcaa
     2101 ttgcaatcgc tttacgcatt gtctccttag atatttcttt tccatcaaaa gaaaaaacag
     2161 tatcgtcttc tgataaatcc acatgtttag gggtttctct ttcttctcta cctagaaggt
     2221 agtctacaga gacatcgaaa tagtcagcaa tccgacttaa ttcatctgaa ttaggagtat
     2281 tatttttcca ctttgctaga taaccgttag aatatccaaa attaatttct aactgccgta
     2341 tagatatctt tttcctcttc gccaagtctt ttattatttc ataggtattc atcgataaaa
     2401 caacctttct aaatgtttac aaaaaaagtt tagaaaaata cgcagaaata atttgactaa
     2461 ttctgagtaa tgagctatac tatatcttgt aaacagattt aacaactaaa aagacaacaa
     2521 aaaacactat tgatatgtaa atgcagaccg ccaagaaagc tttaaaatca atgtttttat
     2581 gtcttattta attatgctct tagtatagag tattactcag atactgtcaa ttgaatttag
     2641 aaaaaagttg ttaaatttgt ttacgaatat aaaagaaagg agagaagaat atggaaaaaa
     2701 cagtctcggc aaaaatcgaa gatttgaaat tagacatact gaaacaagca aaagtggcga
     2761 tggaacacgc ggtagataaa gaagactctg ccatggttgc agccatagca gagattttag
     2821 ctcacgttta gtcatcgttt ttacggtctt ctggaagctg accatagata acagcataat
     2881 gaatatctaa ataagcttga acaatttctt ttggcgaaat tgattcgcct ttagtagata
     2941 cttgtgattc gtgatttagc catgcaacaa ctaaatccgc agcgatttca aacggcgtta
     3001 cactttgtgt cagttgccat aaagaatttc acaatatgta tggtcgtggt aataatacca
     3061 aagagcaatt tgaggaattt atggaaatta aaaaatacaa aaaatcattt attaaaggag
     3121 gctaaacaat gaacaaggaa atgagccaaa caatcaaggt acaaaaaatg atagatgatc
     3181 taacacatgg aattgatagc caagccgata aaatcatcaa ggaattgcaa ggacaaaaag
     3241 ttaaggatgc aaaaatgctt ctcaagacta tcaactttga aatgaatcca acaaaaagaa
     3301 agctcgccga tgtgttggaa gaaaagttgg cttccgctat caacgagcaa gaattactat
     3361 ttgaaaccga tacttttaac agctgatgtt ttatagatga cagtttcatc cgaatctata
     3421 tcatcgccga gagaaaagaa tgttgctttt gacaaaaagc cgataagacc tacaagcggt
     3481 cttgatgttg caagagtaga taccccaata tcaccttgca aggtattcac gtaaatttga
     3541 gaagaataga acattccatc tttttcttcg atatttgacg catcatcgta tccaacgacc
     3601 aaagtatcgg caaatatttt gattttttct ccatcaagca tagtaattac aaattctggt
     3661 ttcataaatt ttcacctcct taacaattat ttcagcctgt cacactgata aggaaattat
     3721 accaaagaaa ggaatgagaa atatgaatac accacaaatt tttaatttcg agcaaaacga
     3781 agttcgaact attttagtaa atgatgaacc atattttgta ggaaaagatg tagcagacgt
     3841 tctaggatat tcgaatccgc aaaaagctat tcgagatcat gtggacttgg aggataagac
     3901 gcagaacgat tcgttcaccg tcaatggaac agcagttgtt ttgattaacg agtcaggcct
     3961 ttacagttta atcttaaaat caaaacttcc ctctgccaaa aaatttaaac gttgggtaac
     4021 aagtgaagtg ttgccagcaa ttagaaaaca tggaggttat ctaactccag aaaaagtaga
     4081 agaagctttg cttaatccag atacaatcat tcaattagca actcaactaa aagaagaaag
     4141 aactggaaga ttaatcgcag aacaaaagat tgcagagtac gaacctaaaa tctcctattt
     4201 agatagcata ttatcttcta cagattcagt aacaattagt cagattgcag cagattatgg
     4261 gatgtctcca caacagatga ataaattact tcataaacta ggtattcaga aaaaagtcgg
     4321 taaccaatgg ttattgtgca aaaaacacat gaaccaagga tacacaaaat ctcatacaac
     4381 tgagatcccg aaagccgatg gtggcactaa aattgtaatg aataccaaat ggacacagaa
     4441 agggcgtcta tttatctacg aattactaaa aaaagaagga tattaccctc aaatggattt
     4501 agaggaaatt ggttagaaag gagttttagt atgactgaca ttgcagaaat cactcaacga
     4561 gatagagaaa aaatcaaaga atatgtcgaa agttcaaagt tcttaactta caccatgctc
     4621 gctgaaagat tcggcattag caaaagctac ttatctttaa ttttaaacgg taaaaagact
     4681 tctgcagaag caaatagaat catagattcg attattacta tgtacgaatt gtaaagggag
     4741 gaacagctaa tgcaatatct agaagcgaaa atcccagttc cagaaggcta tgtaattatc
     4801 tcccaagtgg attatgagga gttaaaaaaa gctgatgata ctggtagatg gatgacgttg
     4861 ccagaagtgc tagaacggat taacagaaaa tatgattggt ttacttctag agttttaaag
     4921 aacccaagat atagaaatat tatcgatatc gaaaaaaaca aaaatggatt tgtctattat
     4981 ccagttgaag gaagagacac atatctattt ttaagaagta aaacacttga atttttagaa
     5041 acaaattttt cggaaatctt aaggaggtaa gcggatgggt aaatttaata gagcactagt
     5101 attcagcgca ccactaatta tctacgcttt aggtctttgg ggaagcaggc aagcgttgat
     5161 aggaacgatt gtttacatgg tttggatttt tatggggctt gatgaagctg aagctgagta
     5221 cagagcgaaa aagccaaccg agggggctga ctaaaaatga atagaaaaga gaaactagaa
     5281 tgtatattac tattactcag tttaattctt tcactaattt ctctattggg aagtttgtat
     5341 ttttgatatc aacaattttg tggatttggg ttctatttag agtaaatatc atttttgttt
     5401 ggtgattaag gagtttatta aaattaattt tccctacttc aaatgcaagg taacaagata
     5461 tagcactttt aggaggaata tttagcggta cagcttcaga gtatattcta ttatcatcga
     5521 ctgatcctcc tttagcaatt aatttcttaa atggagtagc tgaccatctg cggtttaagt
     5581 tagcggatga aagttctaaa ttaactaaca tagctggttc tgttgaatag ttagaaatga
     5641 ttacttttgt ataaatcata tctgcagcga agtaagaagc attaagttca acttctagtt
     5701 gagggcgcat tccttttatt tttatccctg ttaaaaacgt actcaatata aaacttatta
     5761 ttgacatcca ttgaaaaatt gttaagtgaa aaaaattcaa aataaacacc accagttttt
     5821 aactaaatta taccaaaaag gagagaagaa ataatgcaag aattagtaat tttgaaaaat
     5881 aaagaagctg tgactacgag cttacaagtg gcagaaagct tcgagaaaaa acatcaacat
     5941 gttttaagag atattgatgc actaaaaaaa gatgtgtcca attttggaca gatgtttgta
     6001 gaaggtaatg aaccagattc atatggcaga aatcgacgag ttttcttcat tagtagagat
     6061 ggatttttct tgctggctat ggggtttaca ggaaagaaag ctatctactt caaacaaaaa
     6121 tacattgaag cattcaacga aatggaagat gttattcgca agaatactgt tcctcaaaca
     6181 attgaagata tgatgatcta tcaattagaa gaaatgaaag atgttaaaaa agatgtttcc
     6241 atgcttaaag atactatgcg aattagcgga caacaagagt ttgaaattaa gcaaaaagga
     6301 aatatgaaag ttatgggagt tctaggggga aaagaaagcc gagcttatga agaaatcagc
     6361 aaaaaagtat tctcaaaatt ttggtctgaa tttaaacgta ccttttcaat cccaagatat
     6421 ggcgagttac ctcgtaagag attcgatgat gctgtttcat ttattgaaat gtggttgcca
     6481 gaaactgcga tccgtatgga aattgatcaa ctgaacagac aacagagact ttttggtgat
     6541 gacaatgaat agagctgaag cgctaagaat agggacggca attgctaatt gctggtggaa
     6601 atactataaa ccaatcatcc taagccaaca acatattgac aagcaaaaag catggcaaca
     6661 aataaaaaag tgactccgcc ggcaagcaaa gagtcacaaa gaaaacacat cataaggaga
     6721 ttttagcata tggaaaaaga actttccact ctagatcaat atttgattga tcctgattgg
     6781 ggcaagccga aaattgagga aacaagtggt cgaaaaatca gacgaaatct tttgacgaat
     6841 gaagaactag cttgggatca agatgattta ggcaaccatg taactatttg ggatcatgtt
     6901 tatcttatcc atctatcgaa gcattcgaat aaacctgaat atatttacgt catcgaagat
     6961 ggcttgattg atgcgctaga agagtacgac agagataact tgattgatat ctcttattac
     7021 ggaccaggta agaaatacat tgctgaaatg gaggcagaat ttgatgagtg aaatcaaagg
     7081 gacaacgaac tttgaaaaac tttttagtcg taagttaaat aaaattctca agaaaaaagg
     7141 aaattttgat tatttatctt gggctcacgc gtgggagatt atgaaaaaga atgatccaca
     7201 ggcaacggta actattaatg agtacaaaca ctacagggtt gtttctggaa ctcatcaaga
     7261 ctttcttgtt gaggaatata aaccttttct tatggatgaa actgggactt atgtatctgt
     7321 ctcagtaacg gttaaaggac acacggaaac cgaattattt cctgttttag attatcgaaa
     7381 ccaaccagtt gttaaaccaa atgcaatgca aatcaataac tcattgaagc gatgctttgt
     7441 gaaagcattg gctctacacg gactgggatt atatgtattt caaggggaag atattccaac
     7501 accacctaga atcgatacaa agaaattaaa catgctagag acgattctag aagctttcaa
     7561 tgagcagatg ggtaaagata tgaccaaaac cttaatcgaa tatgttaatg agcagacaga
     7621 taaattaggg ctcttagctg ataacgttga aactattgaa cagttaagct atgagcaatg
     7681 tgccttgatg gagcgagcaa tagcagctaa gagaaaagaa ttagataaga agtgatatga
     7741 gtgtttaaac cattaatcga ttcatattca gcggttctga aaaagttcaa aggaaaagac
     7801 ataagcgcaa ccatcaatga ggaagtgaac attgatcgac taaagacgat gtatgacggc
     7861 tacgatggtg atcgagtcat tgaaattcgt tttattgatc ctagacgttt caccgtacag
     7921 caacgaaact tcatctatgc gctgataggc gatattttta tcgatacagg catgccaacg
     7981 gacttctgga aggaattctt ctactttcgt tttgaaggtg tcacagggcg caaaataagc
     8041 ctgaaagacg aatcgaatac aactgtgagt gatgccaatg tcttagcaaa tatcatctta
     8101 gatttcatct ttgaacatca tattcctttc aaagaaggct atgagatttt acctgcgaat
     8161 caagaatatt acttctacaa atgcatcaca aaaagagtct gctgcatctg tggcaaaaca
     8221 ggagctgaca tcgatcactt tgacaaagcg ctaggaagac gaaagcgcaa agaagttgat
     8281 catgcagagt acacatttgc agcactctgc agaatccatc acacagagaa gcacaaaata
     8341 ggtgtgatta atttcaaaaa taaatatcaa atcaaaggga tcaaattaaa ccagaaaaca
     8401 atcaaaaagt taaatattgg agggtaaaaa tgacagaaca tcgaagttat tatgcgatta
     8461 taccagccaa cgtaaggtac gacaaaagac ttaaaccaaa tactaagttg ttatacggag
     8521 agataacggc cttgtgtaat gaaagaggct tttgttgggc aggcaatgag tactttgcag
     8581 atttatatgg tgtgaataaa gagaccatat cgcgatgggt aagtgatttg attaagtttg
     8641 gatacttgaa tcgggaaatc atttacaaag agggtaccaa tcaaataatc aataggtacc
     8701 tacgaattaa tcaataccct attgacgaaa aacgcaatac ccctattgac gaaaaagtca
     8761 aagataataa tacatctatt aataatacat ttaataatac aaaagaatat ataagagagt
     8821 taccgccttc gaaaaaatcg aaggctaagc ccgtccgtca taaatacgga gagtataaaa
     8881 atgttctttt gtcagatgag cagatggaga aactcaaaac agaattccct aatgattacc
     8941 aagagcgaat cgaacgactg tcagagtatt gtgaatcatc tggtaagact tataaaaact
     9001 atttggcaac tattcgaagt tgggcaagga aagaaaaaag tgaacctaag aacgcaagca
     9061 gtggatacaa gcgcacagga agacgagaga agcttcctga atgggcaatc gaccaagaag
     9121 cctatctcaa gaaaaaagcg ctagaacgag ctaatagaca atcaaaagca ccattctaag
     9181 aggtggaaaa atgaagatcg attatctaga actaattaat gaaatagcga attataaaaa
     9241 gggcgaggaa ttagacgtcc tgagagacgt atatgatcaa ctcgaagaag ctggaattga
     9301 aggaattaag aatgatcgtt cgagttggag taagctcaga tactatttcg cactttatat
     9361 cgatacaaca caattaagaa atttagcata taccaaatta ctatttgttg attgtgttaa
     9421 aggcttgcaa aaacatctta atgaacttga gcaggtgtaa tcagatggac ctaaagacat
     9481 ttacagcaca gattgaacta atgcatcaag aagctttaag acaaagtgta tcgtacgaag
     9541 acaagtggct caacacgttc catggcggac gtgagagcgc acttgatcaa gtactcaaat
     9601 tactgaaagg agaatgtcgg gatggataag aaagcggcaa tgcagcgaat tatcgaattg
     9661 acttattcag aagattggca aaatgacaaa gaagctgctt cagaagtgat gaggcttgga
     9721 agagcgatgt gggcagacaa gagcaacaag ccaagaccac gaaaaatcgc aatttggcac
     9781 ggtgacaaac ttctagtgat agggacagct gaacagttag caagtctcac aggcttgcac
     9841 gagaaaatcg tgaggaaaag agcaaggtgt ggctacacag acgctaagaa gagaacgttt
     9901 agatacgtgg aggaatcatc atgacaacag aagaagtgat tcaaatgcgt attcgaagcc
     9961 ttcagcgtga gattgacgat ctggaacgaa caaaggcagt gatggtcaat gaaacggcga
    10021 gaaaggcaat cgatttgcac atagagaatt taagaaggga aatccatcga ttggaggaat
    10081 gagcgtggat aagaaagcag caatgaaaag aattgctgaa ttaaccaagt cagaatcttg
    10141 gcaagaagac aaagaaatag ttgcagaagt ccaaaagctc ggtaaatcaa tgtggactga
    10201 aaagcccaaa cggagaacgc cgagaaaaat tgcaatctgg catgatgacc gaattttagt
    10261 aacaggtact gctgaacagt tatctgaaat tactggatta agcaaaaaca ttatctggga
    10321 tagagctagg agcttatgga ttgattcaaa aggacgacag tttaggtatg tggaggagag
    10381 ataatggatc tcattacaca atacagtgac atcatcctca agaaaatcat gatgaagatt
    10441 cagaaagata aaaaatcaaa agaacgagct gaattagtta agttagaaat ggctgaaaca
    10501 ggagcaggag tgcgaagtag caggcattgg aaagcagcag caaacattga attttattac
    10561 aacgaaattc aaaaagggtt cgatcagatg cgtgagctgg atcggcaaac aaattggagc
    10621 aagaaacttc atcaagatcg tttcaaattt gtagagaagt ataaagaaat attagaagag
    10681 tatttgagga ggacagcaaa tgataaaaaa actcgttcaa ttcagcatgg atttatatga
    10741 tatcgaatca ggagcaacac tatctgtgga atcggaccat ctaatcataa attttggtgg
    10801 aaagcgccag attattttgt gggtagttga tgatgtactg tttccagaaa ttgttcatga
    10861 tttcgaagaa tcaaaagcgg ttgagtttga aatagtgaaa aaagtaatgg aattgattga
    10921 aaaatacgag gaggacagcg aatgataccg aagtttagag cgtgggtaaa acctgatatt
    10981 ttaagcaatc atcttaatgg ggtgattgct gaggcattac ctaattttat ttatggcaat
    11041 tgtttagttt tgagggaaga tttagaagaa gataagcagt ttgaagaaat tattgatttt
    11101 cttgatattg aactcatgca gtccacaggt ctgaaagata aaaatggtgt ggagatattt
    11161 gaaggagata ttgtcaaaac tctcgatgct gatttgaaag taactttttc aacgataaag
    11221 tttgaagaag gcgcattttg tgttgattac aaagatctcg gcacggaatt tgattttttg
    11281 tactttgtag attcaccttt ggaagtcatc gggaatatat acgagaatag cgagttattg
    11341 gaggaacagc gatgaataaa caggaattga ttgataaact atcaagcctt gtaggtagca
    11401 tagaggactt taaaggtata agtacttttc atgctggaaa atatgcgggt ttagaacacg
    11461 cattagaact aatccaacaa ctaaatgaat cgcagaaacc agttattcca caactcgtgg
    11521 caggttggat cgaaaaaagt accgacaatt t
//
LOCUS       DACECZ010000084        11452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.084, whole genome shotgun sequence.
ACCESSION   DACECZ010000084 DACECZ010000000
VERSION     DACECZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.084"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            133..1104
                     /locus_tag="IWO35_002015"
     CDS             133..1104
                     /locus_tag="IWO35_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ0317070.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(1182..1418)
                     /locus_tag="IWO35_002016"
     CDS             complement(1182..1418)
                     /locus_tag="IWO35_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0317071.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(1387..1518)
                     /locus_tag="IWO35_002017"
     CDS             complement(1387..1518)
                     /locus_tag="IWO35_002017"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0317072.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(1594..1977)
                     /locus_tag="IWO35_002018"
     CDS             complement(1594..1977)
                     /locus_tag="IWO35_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0317073.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(2010..2795)
                     /locus_tag="IWO35_002019"
     CDS             complement(2010..2795)
                     /locus_tag="IWO35_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ0317074.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            2973..4073
                     /locus_tag="IWO35_002020"
     CDS             2973..4073
                     /locus_tag="IWO35_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0317075.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4063..4887
                     /locus_tag="IWO35_002021"
     CDS             4063..4887
                     /locus_tag="IWO35_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ0317076.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            4893..5699
                     /locus_tag="IWO35_002022"
     CDS             4893..5699
                     /locus_tag="IWO35_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0317077.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            5696..6526
                     /locus_tag="IWO35_002023"
     CDS             5696..6526
                     /locus_tag="IWO35_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0317078.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            6533..7564
                     /locus_tag="IWO35_002024"
     CDS             6533..7564
                     /locus_tag="IWO35_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0317079.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(7671..8944)
                     /locus_tag="IWO35_002025"
                     /pseudo
     CDS             complement(7671..8944)
                     /locus_tag="IWO35_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            9221..9430
                     /locus_tag="IWO35_002026"
     CDS             9221..9430
                     /locus_tag="IWO35_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317080.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            9480..10196
                     /locus_tag="IWO35_002027"
     CDS             9480..10196
                     /locus_tag="IWO35_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ0317081.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(10098..10618)
                     /locus_tag="IWO35_002028"
                     /pseudo
     CDS             complement(10098..10618)
                     /locus_tag="IWO35_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            10906..>11452
                     /locus_tag="IWO35_002029"
     CDS             10906..>11452
                     /locus_tag="IWO35_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0317082.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEET"
BASE COUNT         3258 a         2144 c         2638 g         3412 t
ORIGIN      
        1 cctctttttt gaccagattt tgaccagtct attttacatc ctagtatatt tatatgagct
       61 aattttatga ttaaatggta agtttttcct tgtgtgtttt agtttaaact agtgtgtaat
      121 tcaaaaatct atatggaagg aacagtatta agaatagaac agggttctct acatgatggt
      181 gctggtttac ggaccgttgt ttatttaaag ggctgcccat tacgctgtgc ctggtgttcg
      241 attcccgaat cacaaagcaa gcagattgaa aaaggatttg gccagaccat gaccgctgaa
      301 gaggtaatgg atgagattga gaaggatgct gttttttact ttcactcgga tggcggagtg
      361 accattagcg gaggcgaggc actggttcag gcggattttg ctaaagaaat tttgcaaaaa
      421 tcgaaataca ttggaatcaa taccgtactg gaaaccagct tttgtggtgc ttataatgaa
      481 atccaaaagg tagctcccta tgtggataca ttgtttgtgg atgtaaaaat gtttaccagc
      541 aagctccaca aacaatggac cggcttagat aaccaacaaa ttttgaagaa tattcgtcgc
      601 tttttaattg agtatcctaa ctgcgaagtg cgaattcgag ttcctgtggt tccaggtatc
      661 aatatgaacc tgaccgagct gctgacgatt gcttgttttg tggcagatct tgatcggttc
      721 gtaccgttag aattactacc gtaccactgc tatggtatgc acggttacca agcattggga
      781 ctggagtatc cgttggcaga cacgccagcg ccttcaccta aagaaatgtt tgcattggca
      841 gatcaactgg ctcgcacggt tcctcatttg ctggtcacag ctttaaccaa aacatttatt
      901 tatttattga atccaagctt tatcttatgg ataaatgaaa gacactcact agctgcagcc
      961 agctcaagaa tagagctttt gctgaagtca gtgagtgtct ttttacgatg ttttttcgaa
     1021 gttaatttgt cgaatgaaca ctggctcaat cggtacatct tcactgtttg tcggctgagc
     1081 tgcgatcgct gctacgattt ctaacccttg gtatacctgt ccaaaaacag tataacgacc
     1141 atccagctcc ggtagtcctc caagctccaa gtatgcttgc tttacattag caggtcagcc
     1201 tgcgcctttg tgttttgcac aataaaaaac tggttgccgt tggtgtgggg gccgtggtcc
     1261 cctaaggcca aggcgccatt aaaatgataa agtccgcgat gcacttcatc agcgaaaaag
     1321 ccctcatagc tggaaacagc ttcatcacca gaaccatcca aagcgccacc ttgaataaca
     1381 aattccttaa tgacacgagc cgaaaggtgt gttgtcataa acgccttgct cagctaggga
     1441 gcgccagttg gctacagcca ggggagcttg gtcaggaaat aagcggagac gaatttcacc
     1501 agcatcggtg accagcacac aaacttcttc ctgctgcggt ttttctaatt gaaagagagg
     1561 aaggtttgca actgaaacgc cttgatcctg ccatcaaata tcgaaaatcc gccactcgac
     1621 cttcgcctga tcaccaaggc gagccattcc caataaaacg gtataggcgg tttcatccgc
     1681 ttgaaaggtc aattgcagct gtcctcgtaa atcgttgtag tcatctgggg cggctacttg
     1741 ataaaaaaga tgaaactgcg catgcaaata ttccaaaggc tgatctttaa aggctgcaaa
     1801 ataagcctga actttttccc gctgcaagct gtcagccacc aaaaaatttt ccagttgttg
     1861 ttttggaacg gctgctaaca aggatcggca aaccgcctca attttcaacg catcccaatg
     1921 tcgcgcaatt aaataatgga tgggtgcctc ttgattgtag tcccacaggt tgctcatttt
     1981 tttcaccact ttcttctatt atataggaat cacaaaatcg gcagcggcgt caggcggcgt
     2041 tcttttcctt gaatgactag caacttttct acctgacagt ctgccaacag ctgactcatt
     2101 tttttaaagc ccagctgata atcttcggca acatgagcat ccgagcctaa ggtaaagagc
     2161 tttccgccga cactttgata caaaggaacg gcataacggt aaaggtcttc gttgccgtat
     2221 ttcaagaagc tcttggcatt taactccatc gccaattctc gttcaacaac ctttttgaaa
     2281 atcgttagca gctgagcctc aaaatgctcc gcgaattctt ccgctgtgaa ggtgaagcgg
     2341 cgtaatccat aatcaaagtg agtcaaaata tctccttctg gaaaatccgt caagacctgc
     2401 aacatctgat caaaatattc cttggtagtt tgaatgggat ctttggttaa aaccacatcg
     2461 tccatataat caaagcggcc attttgatga atacttacca gctttaaatc ataaggatgc
     2521 tgagcgaggt aatccacgat ccgctcttct tgtcctggaa caacgccaat ctcgatacct
     2581 cgcagaaatt tggtggaata ggtttctttt aaagagtcca gtttgtttat ataggctgga
     2641 taatccggaa tgtcatcttt tccggtagat ggattgttta aatcaaagtg atccgtagaa
     2701 acgaaaaact ccggctgata ggctaaatag ttttcaaatt tttcctctga atcaaaggaa
     2761 aaatacgtgt ggagatgctg atcataatac tgcataggga gcctcctttt ttctactatc
     2821 ataccgcaaa agctttagaa ggttaacggg aattcgtcga aatttacaaa ccatatacaa
     2881 cctttacatt gttcatgaaa ccttttcaag aaactgattc tctcattaca aagctttgtt
     2941 agaatggaat tatcgaaagg ggagaagaat gaatgaaaat gaatgaaaaa attcgtaaca
     3001 tgactatcgg tttattttgt gcagcaacag taagtatcct gctgacaggc tgcgtctcag
     3061 gtgaagcaac gaagcaatcg gctgcggcag acgactcttt agatacgatt atagaaaaag
     3121 ccaaggaaga aggagagatt gccagcttgg gaatgccgga tacttgggca aattgggtag
     3181 gcacttggaa cgaccttgaa aaagaatacg gcttaagtca cgtggatacg gatatgtcca
     3241 gtgcagaaga attggctaag tttgaatcag aaggaaaaaa tggaaccgcc gatattggtg
     3301 atgtcggtat cagcttcggt ccgttggctg aaagcaaaga gttaacctta ccttataaga
     3361 ccaactattg ggaggaaatt ccggattggg caaaggatga tgatggggat tggttgttaa
     3421 gctatacggg aaccatttcc tttatcaccg ataaagagaa tgtgaagcaa gcaccgacta
     3481 gctggaagga gctgcaagag ggtgactatc aagttagcgt gggggatgtg acgattgcca
     3541 accaagcaca atttgctgta ttggctgcgg cgattgccaa cggaggtgac gaaaaggata
     3601 ttcagccagg tctgaattac tacaaaaaaa tggcagaaaa aggccgtttg tccaccgttg
     3661 atgccagcgt agcaaatctt gaaaaaggtg aaatcgatgt ggcagttgtt tgggacttca
     3721 atggactaaa ttatcgtgac caaattgact ccgagcgttt tgaggtaact gtgccaacag
     3781 acggctccat cattagcggg tatacaacct taatcaataa aaatgcccct catcccaatg
     3841 cggcaaagct agcacgggaa tatattttat ctgatgaagg gcaaatcaat ttggcaaagg
     3901 gctatgcgcg acctattcgc aaggtagagc tgcctaaaga agtagctgaa aaattactgc
     3961 cggaggatgc ttatgaagcg gcggtgccag ttgaagacaa tgaagcttgg aacaagacct
     4021 ctgtagagct tccagagctt tggcaggcgg aggtactgac ctatgcaaag tagacttgac
     4081 aaactgattg tagttatact agacggctgt cgctacgaca cagccgtcgc ccagcttggg
     4141 ttcatgaatc atttagttga gcaccagcaa gcaagcttat tgaaggtaaa aagtgagctg
     4201 cccagcaatt cacgtccgtt atacgaagta ctgatgaccg gcgttccagc ctatgaaaat
     4261 ggcatctata gtaattacca tgctcagcga tccaaagagt ggtcgctgtt tgaattagtt
     4321 aaggaagctg gcggaaaaac aggagccgcc gcttaccact ggtacagcga gttgtacaat
     4381 caagccccct ataatcgttt ggttgatcgc atccagcatc aggaggagcg actgattcag
     4441 catggcatct tttatagtga ggacacttac cctgattcgc atttatttgc ggatgcccat
     4501 tatttagtga atcagtatca gccagacttt ttggtcgtac attcgatgaa tattgatgat
     4561 gtcggtcaca aatttaccgc cgattctcat gaatatgcag cagcggttaa tcaggcggac
     4621 agattattgg ctatttacct tcctgaatgg ctgcaaaaag gttatcaagt cgtggtgact
     4681 gcggatcacg ggatggatgc gcacggactg catggcggca gcttggctgc acatcgtgaa
     4741 gttccttttt atttgatttc aaataaacgc caaaagctga aggaacaaac tgtaccacag
     4801 ctgctggcgg ctccattgtt ttgctggtta ttaggaattg caccgtcaga gaaaatgcag
     4861 gacattcaag aattgatgag gggatgaaag agatgaaaaa acgcttggtt ctggttcctg
     4921 cctcctttat cttctgtgca tttttactgc tgccgctgct ttttatggtt ctgtgcagct
     4981 tgcacagtga tttaactaac cagtggacat tggccaatta cagtgatatt ttcagcaaca
     5041 aatattacac acaggccttt gttaacagcg tactgattgc tgtcagctcc agtgtgattg
     5101 ggttaatcgg gacgtttatt ttatgtttat gcttaatgaa tctatcgact aaattgcagg
     5161 agaaaatcac ctttgtttcc aatttggcag ctaattttgc tgggattccg ctggcattct
     5221 cttttattat tttgttgggg aatgtaggtg tgctgaaaat gattttgcca tttctagcgg
     5281 acttcaattt gtattcttgg tggggattga ccatcacgta tacctatttc caaattccgt
     5341 taggggtctt gtttttgtat cccagcatga aggaagtgaa aaaggaatgg ctggagtccg
     5401 cacaattgct gggcgcttca tcagcgtatg cttggttcaa ggtggctttg ccttttttgc
     5461 gaccatcgat tgccagcacc tttgtcatct tatttgctaa tggcatgggg acctatgaaa
     5521 cagcgtatgc cctgaccagc agtaatgtaa atcttttaac gattcgaatt gcggctttag
     5581 tgtcgggaga tgtgttcgcc aagcctaatt taggcagtgc gttggcagtc gctttaggtg
     5641 tcctgctggt aacggcaatg ttgattattc aaaagaaagg caaggtggtg cagacgtgaa
     5701 aaagttaata aaaccgattt tgttctgctt tgtcctgtat tacttgctgc cgattattgg
     5761 aacgattttg tatgccagct ccactaaatg gtcaaaatcg cttttaccta gtgattttac
     5821 cttgcaatgg ttccaacagc tgctgacgga cagggaattt attgctgcgg tgggtcgctc
     5881 acttttatta gcaggtgtgg tcttggtgac gattctgttg ttgatgattc ccaccattat
     5941 ttggattcat ttgtatttcc ctcgtttgaa tcgttggctg gaaaagctgc tcctgctgcc
     6001 gtatgcttta ccaggagtga ttttggtaac ggctttgctt cgaacgtatg ctgaaactgg
     6061 tatcccaatg tttgtagtgt tggtaggtgc attgtttatt acggcgttgc cgattgtcta
     6121 tcttagtctt aacaaccaga tgcgcttaat taatctgaag gaattagtgg atgcagcaga
     6181 aaccttgggc gccccgatgt ctacgattat tatccaggtg ctgtttccaa acatccgtat
     6241 cggtgtgacc ctagtttctc tgatgatttt ttcttcggtt tttggggagt acatgctgac
     6301 gaacctgttg attggcggtc gatttcagac ggtacgcatc tatatgctgc ggcggatgaa
     6361 cgaaaacggc catttggcta gcgctgtgat ggttttgtat ttgctgttta tggtgggaat
     6421 tgctttaact acctttatgc tgacttatcg acaaaagcaa cagctgtccc ttaagcagac
     6481 gagggaagag aagcagtcga aaaagaagcc cttaaaggag ttgtgacgga aaatgttttt
     6541 agaaatacat gatctcaatc tgttttttga tgaaaaacag attttagcta ttgatgaaat
     6601 tcagattgaa aagggtgagc tggttacttt actaggtccc agcgggtgcg gaaagtctac
     6661 gttgctgcgc tgtatcacgg gattggaaca accaaagagc ggtgagattg tgctggacaa
     6721 tgagaatatt gccgataagc ctacgaagga tcgcaatatc ggctttgttt ttcaacagta
     6781 tgcattgttt ccaactatga ctgtttttga aaatgtagcc tttggtttga aggtaaagaa
     6841 gctgagtcct gaggtgattc aggaaaaggt ttttgagatg ctatcattgg tggatatgac
     6901 ggagcaggca gataaaaatg tccagtttct ttctggcggt caacgacaac gggtggcact
     6961 ggctcgctca ttggtcactg aaccgaaagt cctgctgctg gatgagccat taagtgcttt
     7021 ggatgcccga attagaaaac agctgcagcg ggatttgcgc gctatccagc aatcgctagg
     7081 gatgacgatg atttttgtga ctcatgacca agaagaggcg atgcgaatca gtgatcggat
     7141 ttttgtgatg gaagctgggc gggtggctca ggtttccacc tcgaaggaat tgtatcagaa
     7201 tcctcagagc cgatttgttg cggaatttat cggcaactat aatcgtttcg aatggtatga
     7261 gctgaatcag tatacccacg agttttccgc taagggcaat cgctgcattt attatttaag
     7321 accggagctg attcaatttg aaccggttcc tcagggggtt aagattccgg ttctatggaa
     7381 ggaatcattt attttaggca acattcaacg gtatgttttt caaacgctgg agggcaaaga
     7441 aattttagtg gatcggttga acgatcggga agagaaaatt agcgatgcct tatacattca
     7501 gctgaaagat attctagcaa tccctgtgga aattgatact cagcattttg cacaacaaag
     7561 ctagtgtata gtcggcaaac atttctggct ctttgtcaaa taggactgat agagtgatat
     7621 aagaacatct aaggtagaat tgattttctg ctttagatgt ttttttatgt ttaataagtg
     7681 atcagatgat tgattaagaa aattctggtt ctcatcctaa cccgtaagct attcgtttca
     7741 atgctttgat gtgggtatgc agattttcta ttttgccatt tgagtaaggc aactctaagg
     7801 ctaaacgaat gccggattca tagtttataa acgctttttt tatatgatgc agttctcttg
     7861 gaaccgactt gggcattgtt tttacaagct gaaagaagat gtccgcttct ttgtcataaa
     7921 aagcttgaag aatttcttga tagtaagcat aagctattcg tagttcatca gaagtagaaa
     7981 gtagacgttc tacgacttca ctttcggtta aataggccca accaaaatta cgccattttg
     8041 tccatttatc ataaacaagc gtgtcctgtc gtttgatcag aagtcgagat aaagccttca
     8101 cttgtcgata gtgtttggct tgtattggat cgtttcggtc gaagcttttc atgactcata
     8161 gtcaaatatc cttaaaactg cgaatgaggt gcttgacgat gtgaaagcga tccacaacga
     8221 gtttggcatg aggaaagcac tcttttatca gcaaagcata agaggcgttc atatctgtta
     8281 cgagtatttt tacgtttaaa cgtgcacgtc gttcaaattg cataaaataa gaaatgagat
     8341 ctttcttctt acgtgaagga agaatatcca gtaatttccc agtatcgcca tccatacaag
     8401 aaaaagaata gggaccgact tgatttttca atgaacgaaa ttcatctacc aatgaacagc
     8461 gaggcaacca atttattttc acgtttgtca tggggagcag agaagcgagt gttcgcgaaa
     8521 cggttgacca agaaacgccg caatgtttag aaatcgtcgt cattgcttgt ttttcagtca
     8581 attcctccaa tattttataa tgtacttgtt tggcgataaa acagtttttt tcaacaatgt
     8641 aggtattcgc cgtaaaatag gtgtgacagc cacgacagag gtaccgttgc tttgccaaag
     8701 caagataagt ccgttgattc gcacacttat tgagaaggac cagcgatttt ttctttccgt
     8761 tcttaacgat cagcccatgt tgattcacct gtttacactg aggacagggc tgaatcgttt
     8821 taatgagcgt ggcagaaaca acgatactgt tgattgaatg tatttttttc ttctcgacat
     8881 gagtgatttg aatgtttcca tctttgattc ctagtaatag tttgataaga tgaagatcag
     8941 gcatacagaa cgctcctttg attgtgttgt ggtgattcaa ttctaacgga gcttctgtat
     9001 gttttgtttt tttatgctca aaaggactga tagaatgatt tactatcagt cctagaaatt
     9061 atagcgccac atttctttca cctttgactt ttcagacggc agtttttatg ctgatactgt
     9121 agagagagca ggacacagct tttgcttgtt ttgggtaagg gggaaagaag atgaccaaag
     9181 cacttatttt tgattttgat gggacgatga ttgacagtga atggatgttt tacgagagta
     9241 ttcatcacta tttactgaag cctatttact ggagcagttc ggctataaaa ttccttttgc
     9301 agactatcaa caaggtgttg ggacggcagc agaactgtcc ttttatgaag ggataagtca
     9361 agcggttggg agaccagtgg atgctgaaca ttttgagcag cggatcagca gcactggaaa
     9421 atcggagtag tctcaattct gaactgcggg agctagacta tttttttgaa gcacatccga
     9481 tgattaagga aaaaattgat tcctttgtga cgatcgatga tcttcaggtc ggcaaaccag
     9541 atccagaagg ttatttgttg tgcttgaaac ggctgagggt tgaacaggct gttgtagttg
     9601 aggatagtcc agtcggtgcc caagcagcac cgcatatgac agttgatgta caagcgttgg
     9661 atgcagattt tttcgccttt agcgggcata aaatgtgcgg gccgacagga atcggtgtat
     9721 tatatggcaa acgtcaattg ttagaacaaa tggagccagt cgaattcggc ggtgaaatga
     9781 tagattttgt caatctttac gatagttcat ggaaagagct gccatggaaa tttgaagctg
     9841 gtactccgaa cattgcagga gcaatcgatt acttgaatga gattggtatg gagaatattc
     9901 atcgttatga gcaagaactg gttgcctacg ttttacctaa attacaagcg atcggcggta
     9961 tcaccattta cggaccgcaa gatcctgaac atcatactgg cgtggttgct tttaatttgg
    10021 atggcatcca tccccgtgat gtggcgactg ctctggatat ggaaggtgtc gctgtacgtt
    10081 caggacacca ttgtgcacaa cggttcatcg acgatttctg tttgactaat tggtcgttta
    10141 aacagtcgat gataatgccg tttgagcggc tccaatgtcg tggaatcttt gagtaagagt
    10201 ttccaatatc gcttgagccg acggtacttc ttctgatttt cagattacgt gattgatttt
    10261 cgtacagttc tatgagaatc gcctgcttca gttcctttgt tagttgatga tgttcatcaa
    10321 ctaagtccgt tttcgcagaa aaggtctgcc cacaatcata gcatagaaaa cgggtacgtt
    10381 ttaattctaa aatagtcaac ttttctttga ctttcaacat ctgagtacgt gtcgtatagg
    10441 aaccgttgcg gataatacga atcgatccac agtgttcaca atgggaaggt gagtcatcca
    10501 atctcccttt aataaactgc gcttgaacgc ctttatattc tttttcaatc agccattctt
    10561 catcaaaaaa taaatgttta tctgttaatc cgagtaattt tctagtatga ttatccatag
    10621 agcgactcct ttggtagggt gatgttttgg tcgactaaat tttaccaaaa gtgagtctct
    10681 ttttgagttg aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt tccaacaaca
    10741 caaaatatta tagaagcctt ttttctaaag gatttcaagg tgtctgtttg catttattaa
    10801 aaaaaagaaa aagtattggc aacagaaggc gtcaccaaca ctagatatta tagagccata
    10861 atatgctgta taatatctag tgtataggct taagtggagg tatatatgaa aatattaact
    10921 gttgaagatg ataatatgat aagagaggga ataagtgaat atctttcaga atttggatat
    10981 actgttattc aagctaaaga tggaagagaa gccttgtcaa aatttaatag cgatataaat
    11041 ttggttatct tagacattca gatacctttt ataaatggtt tagaagtgtt gaaagaaatt
    11101 agaaagaaaa gcaatttacc aattctaatc ttgactgcat ttagtgatga agaatataaa
    11161 attgatgcat ttactaattt agtagacgga tatgtagaaa agccattttc attgccggtc
    11221 ttaaaagcta gaatagattc tttaattaag aagaattatg gacatttaga gaagtttgaa
    11281 tataaggatc tatcggttaa ttttaatagc tatacagcta aaataaatgg tgaagaaata
    11341 gatgtaaatg caaaagaact agaagtatta aaatgtttat tggataataa tggacaagtg
    11401 ttaacaagaa tgcaaattat tgattatgta tggaaagata gcgaagaaac tc
//
LOCUS       DACECZ010000085        10767 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.085, whole genome shotgun sequence.
ACCESSION   DACECZ010000085 DACECZ010000000
VERSION     DACECZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.085"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            79..1125
                     /locus_tag="IWO35_002030"
     CDS             79..1125
                     /locus_tag="IWO35_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0317083.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="IWO35_002031"
     CDS             1474..2106
                     /locus_tag="IWO35_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ0317084.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="IWO35_002032"
     CDS             2694..4133
                     /locus_tag="IWO35_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0317085.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="IWO35_002033"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="IWO35_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="IWO35_002034"
     CDS             4895..5110
                     /locus_tag="IWO35_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317086.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="IWO35_002035"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="IWO35_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5770..6681)
                     /locus_tag="IWO35_002036"
     CDS             complement(5770..6681)
                     /locus_tag="IWO35_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0317087.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="IWO35_002037"
     CDS             complement(6700..7704)
                     /locus_tag="IWO35_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0317088.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="IWO35_002038"
     CDS             complement(7701..9824)
                     /locus_tag="IWO35_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0317089.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..10749)
                     /locus_tag="IWO35_002039"
     CDS             complement(9829..10749)
                     /locus_tag="IWO35_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0317090.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         3293 a         2032 c         2018 g         3424 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccacc
//
LOCUS       DACECZ010000086        10357 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.086, whole genome shotgun sequence.
ACCESSION   DACECZ010000086 DACECZ010000000
VERSION     DACECZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.086"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWO35_002040"
     CDS             6..305
                     /locus_tag="IWO35_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0317091.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWO35_002041"
     CDS             378..1739
                     /locus_tag="IWO35_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0317092.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWO35_002042"
     CDS             1761..2021
                     /locus_tag="IWO35_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317093.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWO35_002043"
     CDS             2035..2787
                     /locus_tag="IWO35_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ0317094.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWO35_002044"
     CDS             complement(2881..3486)
                     /locus_tag="IWO35_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0317095.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IWO35_002045"
     CDS             complement(3539..4513)
                     /locus_tag="IWO35_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ0317096.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IWO35_002046"
     CDS             complement(4891..5670)
                     /locus_tag="IWO35_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0317097.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWO35_002047"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWO35_002047"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0317098.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IWO35_002048"
     CDS             7161..8558
                     /locus_tag="IWO35_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0317099.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IWO35_002049"
     CDS             8639..9079
                     /locus_tag="IWO35_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0317100.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IWO35_002050"
     CDS             9227..9553
                     /locus_tag="IWO35_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0317101.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IWO35_002051"
     CDS             9554..10303
                     /locus_tag="IWO35_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317102.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3286 a         1825 c         2147 g         3099 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacttgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactg
//
LOCUS       DACECZ010000087        10259 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.087, whole genome shotgun sequence.
ACCESSION   DACECZ010000087 DACECZ010000000
VERSION     DACECZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.087"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            142..291
                     /locus_tag="IWO35_002052"
     CDS             142..291
                     /locus_tag="IWO35_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ0317103.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            302..1816
                     /gene="dltA"
                     /locus_tag="IWO35_002053"
     CDS             302..1816
                     /gene="dltA"
                     /locus_tag="IWO35_002053"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ0317104.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1813..3018
                     /gene="dltB"
                     /locus_tag="IWO35_002054"
     CDS             1813..3018
                     /gene="dltB"
                     /locus_tag="IWO35_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ0317105.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3063..3296
                     /gene="dltC"
                     /locus_tag="IWO35_002055"
     CDS             3063..3296
                     /gene="dltC"
                     /locus_tag="IWO35_002055"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ0317106.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3299..4567
                     /gene="dltD"
                     /locus_tag="IWO35_002056"
     CDS             3299..4567
                     /gene="dltD"
                     /locus_tag="IWO35_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ0317107.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4708..5790
                     /locus_tag="IWO35_002057"
     CDS             4708..5790
                     /locus_tag="IWO35_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ0317108.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5790..6593
                     /locus_tag="IWO35_002058"
     CDS             5790..6593
                     /locus_tag="IWO35_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0317109.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6663..7403
                     /locus_tag="IWO35_002059"
     CDS             6663..7403
                     /locus_tag="IWO35_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0317110.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7414..8418
                     /locus_tag="IWO35_002060"
     CDS             7414..8418
                     /locus_tag="IWO35_002060"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ0317111.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8463..10226)
                     /locus_tag="IWO35_002061"
     CDS             complement(8463..10226)
                     /locus_tag="IWO35_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317112.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3289 a         1701 c         2081 g         3188 t
ORIGIN      
        1 agcagtgatt tgatgtgcta tttttataaa agctatgtga caaaactgtt agtaaaaaaa
       61 cggtttctgt aagctagaca catgtttctt tttgtcaaaa ttagttacac tttgtgtgag
      121 gttatatgag gagggaatcc aatgaacaaa tggcgttcta atccaaaatt gaattattgg
      181 ggaaaattca tcattcgaac aattttttat agtgcagtac tgctgttcct gatttatctg
      241 tatcactata aaaatattca aggaggaact tttatctata acgaatttta ggaggaagag
      301 tatggaaatc aaaacgatta ttgaagcaat cgatgattgg ggaataaatg aacctgatcg
      361 agttgcttat caggcggatg agacgcatac ttttggtgaa ttgaaacaag catcagacgc
      421 acttgcttat tatttagaaa aaaaggtaga aggcgatgga ccaattgtcg tttttggcaa
      481 tttggagttt gaaatgattg tctcatttct aggggttgtc aaatctggcc atgcctattt
      541 gccgattgaa gagcatacac ccaaggagcg tatcctatcg atctttcggg tggctaaacc
      601 atctatggtc atcagtatcg gggactggat agaagatatt ccttcggttc ctgtcataac
      661 aaaaaaagaa tttcaagaaa tcaagaagat accagtagga ttttatccca gaaatagcgt
      721 aaaaggtaca gaaaattttt atattatttt tacttctggt actactggag aaccaaaagg
      781 cgtacaaatc agtcatgata atctagtcag ctttgttcaa tggacgatgg aagactttgg
      841 gatcaagcct ggtatgcatt tcttagccca agcgccattt tcttttgatt tatcggtatt
      901 ttctatttat cctgcattag tttctggcgg aatgctgaag ccgttaaata aagcagttgt
      961 tcaagacttt cgtcaattat tcgcgacatt gccagccttg aagttgaatg tgtgggtttc
     1021 tactccatca tttatggata tttgcttgat ggaacctaca ttcaatggag aaaatgtgcc
     1081 ggaattggac atgtttttat tttgcggaga agaattgacg aaaaaaactg cagaaagtct
     1141 acttgatcga ttcccaaatg cacggatata taatacttac ggtccaacag aagcaactgt
     1201 agctatttca agtattcaga ttgaccagca tgtactagat agttatgacc gactaccgat
     1261 tggctacgta aaaaaagaca cgaaagtttc tattgtccaa gaaggtaaac ctgtttcaaa
     1321 aggagaaacg ggtgaaatca ttattgcagg accaagcgta tcaaaaggtt acctgaacaa
     1381 tcccgaaaag acagctgctg cgttcttttg tcaaaatgac ttagcaagtt accatacagg
     1441 ggatgcaggt agactagatg aatatgggct tttattttat gaaggacgta tggatcagca
     1501 ggtcaaatta catggatacc ggattgaact cggggatatc gaacattatc tgctccaaga
     1561 caaccgaatc aaacaagctg tcgtcgtacc aaaataccaa ggaacaaaag tacagcagct
     1621 ggttgctttt gtcgtattag aaaacaaaac gaaacagccg gattttcagt tgactaaatc
     1681 tatcaaagaa caattactag aagttgtaat ggattatatg attcctcaga agttccatta
     1741 tgtcgatctg ctgcctcaga cagttaatgg aaaaatcgat cgaaaaaaat taacagctga
     1801 ggtgaatcct gaatgatgtt acctcatatg atcccttatg cagagccgtt ttattttttt
     1861 cttttagcca ttgcgcttgt accgatcgtt ctatctttat tgatatggga aaaaagatta
     1921 ccagtttatc aatcagcagt gacacttttc tttttatttg tcagctttgg aggtaattat
     1981 tggcaacagg gagcggcgct gattgggtat gttttgtggc agacgatact tgtatggggc
     2041 tattttgttt atcggcaaag gaaaaatcag acaggatggt tttttgccgc cgttatttta
     2101 gcgataattc cactgttttt aacgaaagtg actccttttt ttgaacatgg aaaagcatcg
     2161 ttacttggtt ttttaggaat ctcttattta acctttaaat ctgtacaaat gatcatggaa
     2221 ataagagatg gcatgatcaa aacatataag ccatttcgtt atatccagtt tctgctgttc
     2281 tttccaacaa tctcttctgg accaatcgat cgctatagga gatttgaaaa agatcagctc
     2341 catccgcctg ttcgtgataa atatgttgat cttttgggaa aaggcgtcca aaatattttt
     2401 gtcggttttt tatataaatt cattatcggt tattattttg gtcaagtact gcttcctgta
     2461 gtgggacgca ttgccttaca gcatggtggt atttcatggg cattagtagg ttatatgtac
     2521 gtgtacagta tgtatttgtt ttttgatttt gctggctata gcttatttgc agtcggtaca
     2581 agctacatga tgggatatca gacaccaatc aatttcaata aacctttttt atcatggaat
     2641 atcaaagaat tctggaatcg ttggcatatg acgctgtcct tttggttccg tgattatgta
     2701 tatatgcgat tgatgttctt catgatgaag aaaaaatttt ttaaaagcaa gatcgtaatg
     2761 tcaaatatcg gttattttgc tttgtttctt ctaatgggta tatggcatgg attgacgtgg
     2821 tactacatcg tatatggact gtatcatgct tgtctgatct gtctgacaga tgcatggatc
     2881 cgatttaaga agaagcataa agagcagttg ccttctaaca aatttacgca tggcttagcc
     2941 gtatttttga catttcaagc agtatgtttt agcttcctga ttttttcagg atttttagat
     3001 aaactttttt ttagctaacg aatagtgaca tattaattta tataacaaag gagaaaagga
     3061 aaatggaaga acaaatttta tcaatcttag aagaaataac aggaacagat gaagtaagaa
     3121 aaaatcgtga tattgatcta tttgatgaag ggttgcttga ttctttagca tctgttcaat
     3181 tattagtcga gctagacgga gagctggata tccaagtccc tgtatctgaa ttcgagcgag
     3241 aggattggag cacaccgaat aaaatcattc aacaagcaac tgcattgaag ggttgagtat
     3301 gaaaaagaaa ctttttgcta ttttcggccc ggttgtttta gctgttggct tactgtttgt
     3361 gttttttacc tcctcctatc aagtagatat ctctaatcca gaaacgatta gacgtgcctc
     3421 ttcttccatg tcagtgaacg tgttgaaagg agatgcgata aaaaattatg cattatctga
     3481 aaaacaatat attccttttt ttggctcaag tgaattaagc agaatcagtc catttcatcc
     3541 gtctgttctg gcagaaaaat accagcgaaa ctatcgtcca tttttgctgg gagcacccgg
     3601 gacacaatcg ttaagccaat atatgatgat gcgttctgca ggagatgcta tgaaaaacaa
     3661 gaaagtcgtc tttattattt ctcctcagtg gttcgttaaa aatggtgtaa aaactgatta
     3721 ctttaatacg tattattctg aacttcaaac atacgactgg ttgttttcaa tgaagaaagt
     3781 aacgcctgct gatcgctatt tagctagaag attattaact ttctcaaaag taaaagagaa
     3841 tgatacgttg acggcgattc tgcaaacgat caaaaaaggg aagctacctt tacctgaatc
     3901 tttgaatcag ctgcgcagcc aatggaacat gctgaagaga gaggatgaag tattcagcaa
     3961 tattgggttg aaagatcgac agcaaaaaat cgatcatgag tcaaaacgat taccaaaaca
     4021 ataccaagaa acagaattat ccattttagc aaatcaaatt ggagaacgag aaacgacgaa
     4081 taatccattc gggttgaaaa acgattttta tacgcatcgt atccgagccc acgagccaga
     4141 gctcaaacag tcacaaaaaa attgggatta tcgtttttcg ccagaatttt ctgattttca
     4201 gctggtcttg gatcaattag caaaaaacca taacgaagta ttgttcatca tcccaccggt
     4261 taacgagaaa tggagcgatt ataccggact atctcaagaa atgttacaag gatttgccaa
     4321 aaagatcaaa tttcaattaa atagccaagg attcaatcga atagctgatt ttgtcaatca
     4381 agcgggtacc aactatttta tggaagacac gatccatcta ggctggaaag gctggttggc
     4441 tgcagatcaa cagatcagac cgtttttaga agaaaatcat attactgcta gtaagtatca
     4501 tcttgacgat gcttttttta gcaaatcttg gcaacatcaa ataccagata aactacaatt
     4561 aaaataaaga aaacaggcga gtgttgctcg cttgttttct ttatttttga atattctgaa
     4621 taaaagataa aaataccaag tttgcctgaa caaaaatcat aagcaatggt ataatcacaa
     4681 tatgaatata gaaatggggg aaaacatttg gatacaaaag aagaacagct acttattgat
     4741 ttaacttctg caaaaggagt acctggaaat gaagaagagg taagagaagt attcagagaa
     4801 tatgccaaac cattcgctga tgatattttt tatgacggtt taggcagtgt gattgccaaa
     4861 catggtgctg gcggaggccc caaggtcttt atttctggac atatggacga agttggtttt
     4921 atggtaacaa aaataaccga aaaaggattc ttggaattcc aaactcttgg aggttggtgg
     4981 gggcaagtca tgcttgctca gcaagttgaa atcaaaacac gtgaagggaa aatcgtccac
     5041 ggtgtgattg gttccaaacc accacatgtt ttaacacctc aagtaagaaa taaaccttat
     5101 gaaataaagg atatgttcat tgatattggc gcatcaagcc aagaagaagc caaagagtgg
     5161 ggcatacgtc ctggagatat ggtcactcca tatattgaat acaaacgaat gaatggcagt
     5221 aaatatttac tggcaaaagc atgggataat cgtatcggga cagctgtttc tctgcgagta
     5281 ttagagaatc tatctaaaga agcacaccca aatgttttat tcgcaggaag cgatgtgcaa
     5341 gaagaagtag gcttgcgagg tgctcgaaca agtacacacc ttgtaaatcc cgatattgct
     5401 tttgctttag atactggtac agcaggagac actccaggaa tgacaccaaa agaagcagat
     5461 tcgattttag gaaaaggccc acagattttg atttttgatg catctatgat cccgcataaa
     5521 aaattattga attttgttat tgatgtagca gaggaaatgg atattccatt ccaatataca
     5581 gtgatttctg gcggaggaac tgatgcagga caaatgcatt tgacaagaga tggtgttccg
     5641 tctgtagcca ttaccgttcc tgttcgttat ctgcattccc atacatcagt gattcatgaa
     5701 gatgattatt tgaatacagt gaaactagtc acagaattag tgaaacggct tgatagtgaa
     5761 acagttgaaa aaattcggag ttatgaataa tgaataatag aatacctatt tctgaatatc
     5821 ggaaaaaagc acgagtttct ttggaaaatc aatggggaat caatacttgg cttgttttct
     5881 tatctataat atttacgagc gtcatccaga acttttttga cggtttcagt ccttttccgg
     5941 aagaaagcat gcagcagcac ttattgagtt ttctagtcga taatttactt ttattcgcat
     6001 tcaactatgc attgttttac attgcgctag tagttgtacg gggtggcaga ccaaaaagta
     6061 accttttatt ttcagttttc caaagaaagt attttggttc gatacttggc atcaatttga
     6121 tcaattcagt catcaactgg ctactcaaca gtttaatctt tctgcccatg tttttaatca
     6181 gcggagtgag tacttatatg cagctgcttt tcagttcaaa tgcggtacct acgggtgtca
     6241 tgccagaaga ttttcttgat atcagctttg ttttatcgat gatagggatg atttttgttt
     6301 cagcactcat catgagtatt gttggcggtt tattccaatt tgctgcatgg acaaagttcg
     6361 actatcctga tttgtcggtt attcaatgtt tgaaatatgg ctggttttta ttgaaagata
     6421 gaattggtac gtatattttg ttgcagctgt cttttatcgg ctggtatttg ctaggagcaa
     6481 tcgctttgtt catcggtctt ttgtgggtca ttgtttatgt gaatgtgtca gttgcagaat
     6541 tttatgaaca agcacggatt gaaaaaggag gtccagacgc ctattttact tagcatgtaa
     6601 gcagtttatt catcaaaaaa ataacttttg tggtaggata aatctttgag ggggaatgta
     6661 cgatgaaatt aactgtttta ggatgcttag gagcgtatcc ttataaaaat caagggacaa
     6721 caggttattt gcttcaatct gacgggttca atttgttgat cgatgcaggt agtgctacgc
     6781 tgatcaaatt acaagaacac cttgatccac ttgatctaga tgctgtgatc cttagccatt
     6841 atcatcatga tcatattgct gaccttggtg tattacagta ttactggcaa cttcatccta
     6901 agagaaatcc ggagacattg ctttcaattt atggacacac aaaagattcc attcactttg
     6961 aagaattgac gttggaaaat gtctcaaaag gacatcctta ttttgaagct gaagaattaa
     7021 aaattggtcc ttttctgatt acttttatgg aaacaatcca tccagtagta tgttacgcca
     7081 tgcgtatcgt agaagaaaca acggggaaag tattcgtatt tacaggtgat tcaggttatt
     7141 tggaaagttt cgctgatttt gcgaaagatg ctgatttgtt tctagcagat acctatcttt
     7201 ttgaaggaaa tgaacatcat catgcccatt tcacttcaaa agaagctgga gaacttgcga
     7261 aatcagctaa cgtaaagaaa cttgtactta cacacctacc tcagtttggt gatttagaac
     7321 aattgaaaaa tgaagcgcaa aaggcagcag gcaaagggat tgatatagaa cttgctgcag
     7381 ttgacaaagt gtttaacatt taggaggaag aacatgattt ttgtaccaaa tgaaaaccac
     7441 gacccaagaa tcaatttagc aattgaacaa tatttgctgc aagaaatgcc tttagatgaa
     7501 cctattttgc tcttttatat caatgaacct tcaatcatca tcggcagaaa ccaaaatacg
     7561 cttgaagaaa tcaatcgaga ttatgtggaa gaaaatggca ttcatgttgt ccgtcgctta
     7621 agtggaggcg gagctgtcta ccatgatttt ggaaacctga actttagttt tatcatgcct
     7681 gatgatgggg attcattccg tgactttgcg aaagtaacac agccaatcat ccaagcactt
     7741 catgaattgg gtgttgctgg ggcggaattg aaaggccgaa atgatctagt cattgatggc
     7801 atgaaatttt ctggcaacgc gatgtatgct accaacggtc ggatgtttgc tcatggaaca
     7861 attatgttcg actctgatat caatgaagta gtcaatgcat taaaagtaaa aaaagacaaa
     7921 atcgaatcaa aaggaatcaa atcgatccgt tctcgtgtaa cgaatatcaa accatttctt
     7981 ccagaagaaa agcaaaaaat gacaacagaa gaatttcgtc aagaaatctt gttgaaaatt
     8041 ttcggcgtag agtcagtaga agaagtaaaa acgtacgaat tgactgatga agattggaaa
     8101 aaaatcaatc gaatctctga ggaatattat cgaaattggg attggaatta cgggaagtct
     8161 ccagatttca atttctctcg ccaaaaacgt ttttctatcg gttcgattga agtccatttg
     8221 aatgtctcag aaggaatgat caaagaagca aaaatctttg gcgatttctt cggactagga
     8281 gatatctctg acgtggaaaa aaaattggtt ggacagaaat atgataaagc atctctatca
     8341 aaaatagtca gccaaattga tgtgaagaaa tattttggtg cgatagaacc agaagaattg
     8401 cttaccttac tttattgaaa ataaaaaata gcggaatcca catatatgga ttccgctatt
     8461 ttttatacag gattcgggta atttcgagag agttgaacag tgatccgatc aatgaaagta
     8521 tctttttcct gcgttagtgt tcctgtgtaa cgatccgcta cgtctttcat catcattaag
     8581 tcaaaacgtt cctgatcatc taattcttct gtgatgcctc gactaaatag gtttgtatcg
     8641 taatgtgcac tatgttcgca acaaatgacg aaatgttcct cttgttcgtg tatcgacaaa
     8701 taaatttttc gatacagtgg attttcgatg gcatgagttt ctctaaagga atgctctaaa
     8761 gcgtggatca aaagttgagc cagatcgtca tcgttgatag gaagttcttc gggaagatct
     8821 gcttggaagg ttatctgaat attccgtttg gcagctaact tctggtaacg cgctaagata
     8881 agatttgtca cctggtggtt gcaaaaagaa cacagcattt cgaaattttt tgcttcttct
     8941 aatatctttt ccacatagtt tttagctttt gtttggtccg tatgcattaa atcttgcaga
     9001 ttctccatct cttggacaaa ttccaggcga agtgtattta cttcttggat atgctgtcga
     9061 agctgctcat aatgttgctc caacaaatca tttttgattt ttaagaaatc tacttgtctc
     9121 cgatctttgt cggtatttaa gatgtgatcg attagattgt agctgataat aacgattaga
     9181 atgataatat ataaaatcgt agaccaattg atcgttgttt gatagatacc taatgccgtt
     9241 tggatgtaaa tcaagcaatg gataatggct accaagacga tccaaggtgt acaagctaca
     9301 taaaaacggt tattcttata tgcttcgcca atgcatgcaa gtgaagtgac taaagtgctt
     9361 ataacactag caatattagc aatcggcatt aattcaggaa gttctgcagg agtaaaagtt
     9421 gcataaagca gaacgccgat aaaaatcaaa agttggaacg tacaaaaaat tccgtaccat
     9481 tttttatagt aaaacattcg gctccaatag tagcagatga tgaaagctga cgttaagata
     9541 ataaataaat gagataaaaa agaatgaacg aatggttcaa aaagaatacc acttaaaata
     9601 tcttctgata cagcttcaaa tcctaatgcc aaagcaaagc aacttaagag aataagtgag
     9661 tattcaagac gtttttgttt atacatcaag tagatcgtat agaaaaaggt ccctatagcg
     9721 ataaaaacag cgattatcat cgtcaatact tgcggcactg attgtgaata aataaaacta
     9781 atcaatggtc cagttgttcc tagataaact ttcggtggca gtccagcata gttctgataa
     9841 ggagatgaga ctgtaagggt caattttttt ccgatataat ttgctggtaa atctatcaca
     9901 gccaatgaga gtccgggatt gctttcttcc tgataatctt tccgttggta taaaatcttt
     9961 tcgcctagat atacagtaat ccattgatgg tttcctttga ttagtaaatt tgcttcgtcc
    10021 aacggttttt caagtgtcgt ttccataatg aatgtttcgt ctttatccac ggtaggaatc
    10081 gtacgagtgt acttgctgtc aaacttaaaa tcaggatcat cttttgtata gaaaacccac
    10141 gaatctttta tttctttcat ttgattgctg tccgcaggag aaaaaataaa ataaaagaaa
    10201 taagcaattc ctgttaaaaa taacaatgat aatataatga ttatgctcca ctttatacg
//
LOCUS       DACECZ010000088        10013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.088, whole genome shotgun sequence.
ACCESSION   DACECZ010000088 DACECZ010000000
VERSION     DACECZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.088"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(476..700)
                     /locus_tag="IWO35_002062"
     CDS             complement(476..700)
                     /locus_tag="IWO35_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317113.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            900..3668
                     /locus_tag="IWO35_002063"
     CDS             900..3668
                     /locus_tag="IWO35_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0317114.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            3750..4169
                     /locus_tag="IWO35_002064"
     CDS             3750..4169
                     /locus_tag="IWO35_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ0317115.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4181..4651
                     /locus_tag="IWO35_002065"
     CDS             4181..4651
                     /locus_tag="IWO35_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0317116.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4668..5447
                     /locus_tag="IWO35_002066"
     CDS             4668..5447
                     /locus_tag="IWO35_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0317117.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5434..6255
                     /locus_tag="IWO35_002067"
     CDS             5434..6255
                     /locus_tag="IWO35_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0317118.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6272..7321
                     /locus_tag="IWO35_002068"
     CDS             6272..7321
                     /locus_tag="IWO35_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0317119.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7334..8338
                     /locus_tag="IWO35_002069"
     CDS             7334..8338
                     /locus_tag="IWO35_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0317120.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8971..9990
                     /locus_tag="IWO35_002070"
     CDS             8971..9990
                     /locus_tag="IWO35_002070"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0317121.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3360 a         1650 c         2093 g         2910 t
ORIGIN      
        1 ttgcaatctt ttttgctacg taatttttgt aaaaaagtgg gttgagagaa atctaattga
       61 ttttcaaaga aggagtatga aaatgagaaa aatatttatt cgtttattgt gggtactatc
      121 ttagaggaat aaataatagt ggagaaaact atggtttggc gcagattcta tcaggtttga
      181 tggaacgaag acaacctccc gacatcaagt caaaagctac ttgagatttt gatctgaaaa
      241 taaaaaaaga aaacaaggta ggaacatcaa tgtttctacc ttgttttcgt aatcaatggg
      301 aaaaaactaa gagtggtgta cgtaatcaat aaaaaggatg gtgacaacag tacctcttta
      361 ctctctgaaa gcatcataac tcgtttttcc tcattatgcc aataaattgt tgttatacca
      421 catataagga tttgtaatag ctaaaaacag acaggaaagt tgaaatctgt gaatcttatt
      481 ttgtatggaa ggatttatag aacgcaaaaa tcaagataaa gatgaataaa aatcctgttt
      541 cggctatata ttgcgtatct acgccaataa aacgagtttg ttctgtcata gccatataaa
      601 gcaaaggcaa aaatacaact acagcgagat aagatccgat gatttctcga aaagttttcc
      661 aggaagtatg tttcctgttt ttatatttat ccgatttcat tgaatagtca tagcctcctt
      721 attctttcat atgttgaaat tccagtataa aaactggaag gaactgtacg ttttttagaa
      781 taaaaattat aagggataag taggctgctg tctataataa gatatatttt tcaattcttt
      841 attaaagatt ttattgacaa aatgaatccg ttttctatga tggaggaggg gtgaataagt
      901 tgttgaagaa ggaaatttta tgttacttag agaaccagac agcctttttc gatttagaac
      961 atatgaacga ggtctttaca gcacaaaggt tagctgatca ttttggggta aaaagaaaca
     1021 cagtcagtca ttatttgaac cagttaaatg atgagcaact actagtcaaa atcaattctc
     1081 gaccagtgat ttatttccac aaagaagctt ttgaaaaaca attttttaag ttacgcacga
     1141 atttttattt ttctatcgat gatctcaaag aggaacagcc ttttttcgcg caaccaaaag
     1201 acttattctc ttttatgatc ggatacgacg ccagtttgaa agaaagtatc gaacaaatca
     1261 aaacagctct ttactatcca gatggcggat taccactttt gattacaggc gaaagtggca
     1321 caggtaaaag ttatttagtt aatttggtct atcagtattg tctgcttcat gatctgctag
     1381 aagattctgc accattcatc acttttaatt gtgcacaata tgcagataat cctgaacttt
     1441 tgactagcaa tttgtttgga catgttaagg gagcgtacac tggtgcagaa gaaaataaaa
     1501 aaggggcttt tgaagcagca gatggtggga tcttgttttt agacgaggta catcgcttaa
     1561 caccagaagg tcaggaaaaa ctgttcacct atcttgatca aggtgtgatt tatcgtatgg
     1621 gagaacctaa tttgtcacgc cggatcaaaa cacgtctctt ttttgctacg actgaagaga
     1681 ttactagtca tttcttaaca actttcattc gaagaatacc tatccgaatc gaactacctg
     1741 cacttaatca gcgtagtcgt aatgaacggc tagaactgat ttactctttt tttctcgaag
     1801 aacaaagaaa aatggggagc cctttgtcag tcagtggacc agctttgtca cttttaacaa
     1861 gtcagaaatt atccggaaac ataggagagc tgaaaaatat cgtcaaagtt tcagcagcaa
     1921 aagcatatgc tgaacagcgt gaacagcctg aaatccatgt cacgatccat catttgcaaa
     1981 aagaactact tgcgcagcct attactaaaa atacagcaca gcaaggaatc tatattacgc
     2041 aagaccagac cttagagcaa ttaatggaga agcaacagcc cgaacagcag cgaatcgtta
     2101 aaagttttga gcaaatccta attgatttca agaaaaataa ctgtctgcta tattcttgcg
     2161 aaggaaaatt aaagcaagaa gtgattcaat tatttgattt tttgctgttt gagaccagta
     2221 gacaagaaaa acacgagctg ctggtttatt ggacacaatc tatccgagag acctttcgac
     2281 aaatggaaac tgcctatcaa atcaaattcg atggaaacag tgtctatgcc ttgagttact
     2341 atctctatca gcgaagcact atcaaatggt taccggaaga acatgatatt atccaactaa
     2401 tcaaagaatt agaacaacaa gtttctcagt cttatccatc tagttatcac tatgtccaac
     2461 gaattctgga attaagtaag atcaaattag atattgaaga cacaagtatg gatcggattt
     2521 tactgacgat ctatctaaaa aaagcgaaat ggtcaaaaga aaaacgactg ccaaaagcga
     2581 tgattgctgc tcacggttat gcaactgcta gcagtatggc aaatgtagta aatcgtttat
     2641 tacaagagga tctatttgaa tcatttgata tgccactaga tgtcacacca cagcagattg
     2701 cttcagaaat ccttgattat tgcgaaaaca gcgatgtttc aaatggattg atcatattag
     2761 tagacatggg atctctgaaa gaaatccatc agttttttaa aaaacagtta tccgtaccgc
     2821 ttttgatttt gaataatgtg acgacacctt tagcaatcac agtaggggaa tgcctgcaaa
     2881 aaaatgtatc attagaagag atagcagaag agactgttcg acagatccag ccagaatggc
     2941 gactgctcta tccagaggaa aacaaaccca aagcactcat tactacttgc ttcactggaa
     3001 tcggtacggc ggtccactta agtgaactgc tagaaaaaag cttacctacg gcttgtcagt
     3061 tgaaaatcat cccatatgaa tatcagcaat taaaagataa gaaaaacagt gacccattat
     3121 tttccatata cgaagtcgta ggaatgattg gcaccacaga tcctgatatc accaatatcg
     3181 actatctttc attagaagac ctcatctcgg gggaaaagat gtctgtacta gctgaatggt
     3241 tggacactac tatgaatgct tcagagaagg aaatattcaa tcagcgcatc attcgtaatt
     3301 tttcattgga aaaagtactg gattccgtga ctattctaga tacagagaag gtgatgggag
     3361 aaatcgatca ttttatgaga gaattagagg tgcagttgaa tcgaagcatc agcaatccca
     3421 aaaaattagc gttgtatgtc catgtgagct gtctcattga acgattgatc cgacaaatgc
     3481 caatcgaaac ctatcaagga ttagagaaat ggaagcagtg tcaaaaagaa gggttatctg
     3541 ctattaaatc tgcgtttagt gtcattgaag agcattatag tgtcatgatc cccaattctg
     3601 agattgccta tatctatgat attttgagtg aagatacaga gtttctctcg atagaagaag
     3661 aattttgaac ataagcaagt tgttgccact aattttggca tgcaacttgc tttttttagt
     3721 atgtgtaagt gaaaagagga gatgaaaaag tggatagaga aattatctta gcttcccatg
     3781 gaaaatttgc ttcagggata ttagattcat tagagctgat ctatgggaaa aatcattcta
     3841 ttactgtatt cgattgctat actgatgaga attttgattt agcggaaaca gtaaggcagc
     3901 tttttatgag gtacaaagat aaagaaatca ttgttctgac ggatcttttt ggcggaagtg
     3961 tcaacaacga attcttacaa tatatcaatc gggatcatgt ctatctcgtt gccggattga
     4021 atttgccact attgattgag ctagtatctc ggttggacgc tgaagtagag acaactgttt
     4081 tgatccagca ggtgctaact gattcaaaaa aaatgatcca gttttgtaac gagcggctca
     4141 atcagaaaat agaagaagac gaattttagg aggaagaaaa atgattgtat tgacaagagt
     4201 cgaccatcgg ttattacacg gacaagtagc tttttcatgg acacaaacaa taggcgcaga
     4261 ctgtattttg attgctaatg atgatgtacc tacgaatgag atacgaaaga caacgatcaa
     4321 attagccaag ccacaaggag tcaaactagt gatcaaatca attgatgact cgattgccgc
     4381 gttgaaaagc ggggtgacag ataaatataa gttattcatc gtagtggaat cgatcgagga
     4441 tgcatataag ctggcagaag cgtatccaga aatcaaaacc atcaacttag gagggatcaa
     4501 ggcaaaacct ggtactcgca gcatcggaaa agcggtaaat attttaccag aagaggagga
     4561 acgcttgaaa cgattaattg acaaacaaat agaagtagaa atccgtcaag taccagcaga
     4621 taaaaaagtg gatgtaagag aagttttgta agaaaggaag aacctcaatg attgtacaag
     4681 cagtcttatt aggaattgtt gcatttgtcg cacaatcaga atatgcacta gggacatcgt
     4741 tgctttctcg tccgattgtg acggggctgt ttacaggaat cgtgttaggt gatgtaaaaa
     4801 ctgggatcat tatgggcgca actttagagt tagcatttat cggatcgttc tcagtaggtg
     4861 cttcgattcc acctgatgtt gtgaccggtg ggattttagg gacggctttt gctattacag
     4921 ctggagccgg tacagaaacc gcgcttttac taggtttgcc gattgctacg ttgacattga
     4981 tattgaaaaa tatttactta gggcttttga ttccaattat gaaccataaa gcagacaatt
     5041 atgcagaaga aggaaattat aaaggagtcg agcatatgca cttgcttgcc ggtatcggtc
     5101 tttccttgat gcttggtgtt gtggtaatgg tttcttatat ggtgggaagc aatacgatcg
     5161 gaaatctatt aaatatgatt cctgacttcg ttcaaaaggg gttgtcagta gctacagggt
     5221 tgattccagc tcttggtttt gcgatgcttg ctcgtttgct gatcaacaaa caagttgcac
     5281 catatttttt cttaggtttt gctatagctt cttacttaga gattcctgtg accggagtag
     5341 ctatttttgg tgcgatttta gcagttgtgg tagtcaatat tatgaatgtc cgtcaactac
     5401 cacttcaaac aactgcaggg gaggtagaag aagatgaaga attctgagat caaaaacagc
     5461 aaaataacga aaaaagagct aaatcacgta ttttggcgtt cttttcaaat ggagttttct
     5521 tggaattatg aacgtcagat gaatatggct tatgtttatg caatgattcc gattttgaaa
     5581 aagttatatc agtcgaaaga agaaatggca gcagctttga aacggcatct agagtttttt
     5641 aacacgaccc cacatatcgt tacactgatt ttaggaatca cggctgcaat ggaggaagaa
     5701 aataaggatg atcctgaatt tgatgtgact gcgatcgata gtatcaaaac atctttgatg
     5761 ggacctcttg cagggttagg agattctttc ttttggggaa ctttgcgatt gattgccaca
     5821 ggtgtgggta cctctttagc catgcaagga aatattttag gccctatctt gttcattttg
     5881 attttcaata ttccgcacat tctgtttcgc tatctagcaa caggttgggg atatcgctta
     5941 ggaacgggtt ttttgaaaaa gatccaggca aacggtatga tggaaagttt gacattaggc
     6001 gcgtctatta ttggactgat ggtcgttgga gcaatgactg ccacgatgat caccatcaat
     6061 attcctttaa aaatcggttc aggagaaaat gctgtgactg ttcaaagtat ttttgatgat
     6121 atcgttccaa atatcttatg tcttggagca tttggcatag tgttttatct attgaagaaa
     6181 gaagtgaaac cattaacgat cttgatcgga ctagctttct ttggaatttt tggttcgtgg
     6241 atagggattt tttaaaaaag gagcagagaa aatgtttacg atgcaagatt atatctatga
     6301 agaaaaggaa gtactaagta cgatcttgaa aaagaatgat ttttcgacaa gagaacatat
     6361 gaaaaaaaca gtgaatttac tgatcttagc aacgggttca tcttataacg cttgtttagc
     6421 agcgaagcca gcattggaat cttatggtga tcttactgtc gatattcaag aaccttttta
     6481 ttttttcaat tatggaaaac tttctccttc aatcgatact gtgattgctg tgtcacaaag
     6541 cgggaaaagt gcttctactg ttgaagcagt aaaaatgata caaaaacaag ggttacctgt
     6601 tgttgctatc acaaatgacg tgcaaagtcc acttgcttta gaagctgcac aaattattga
     6661 tttaggtgcc ggggtagaat cagtaggctt cgttacaaaa ggctattctg cgactgtttt
     6721 acagttacta ctcttaggga taggaattgg aataagtaaa aataaaatca gcaaaaaaat
     6781 agaacaagac tacatgcaac agttgcgcaa aataatcaac catttaccag cgattattca
     6841 aaaaacggaa gaattttttg atggatatca aagtttattt cgtttagcac agcgtttcgt
     6901 caccattgga tatggtccaa actggggaac agctaaagaa gcagagacga aattgactga
     6961 aacaatccgc gttccgtcac aaggatttga gttagaagct tatatgcacg gcccatacct
     7021 ggaggcagat gcttcgcatt tgctattctt tattgaaggg gaaagtgtaa ataaggaacg
     7081 ttcgcaaaag ttgcaaagat atatgagtcg gtacgttgga gagactctca ccattaccac
     7141 aaaaaaagca agaaacgaga aaacacttgg attagcaata gaatgtgatg aatatctgtc
     7201 tgtattagcg cttgtcgttc cgtttcaact attcgcttat aaaaccgcag tagctaaagg
     7261 tattgacttg aacaaaaaga tttttgaaga ctttgataca gtcttgaaaa gtaaattata
     7321 agaggtggaa atcatgctga aattcaatga agaagaacaa atcaaagcaa taaaaggcgc
     7381 actagcttta cgtccgcaag tagaagaaat catcgataaa ctttatgcgg aaaaatttga
     7441 cgctgtctat tatctaggaa tcggtggaac gtatgcttcc tcgatgcaag ctgtcactta
     7501 tatgaatggg aaaagcaact tgcctgtctt tgtacagcat gctgcagaat attacactac
     7561 tggaaacaaa agattaacaa aagattctat cgttattttg tcctctgtta ccggtacaac
     7621 tcaagaagta gtgaaagcag tagaacaaat caagaaagta ggagcgacac tgattggatt
     7681 tatcgataaa gcgaacagta agttgtctca gctatgcgat tttgtggtca cttatccagc
     7741 tcctggaaca gaacaaatca aattttttat ggcagcagat cgattgatgt atttgaatgg
     7801 agaattcgaa gcttattcag aatactatga acagctcgaa cgttatttac caacagggtt
     7861 agtggaagca gagaaaaaag cagatgcatt tggcttatca tttgcagaaa aacatcgacg
     7921 tgatagtatg cattatttta ttggagcagg gaatcaatgg ggagctgttt attcatatgc
     7981 catgtgttac tgggaagaac aaagctggct tccatcgaaa tctatccatg ctgccgagtt
     8041 tcttcacggg acgttagaga tcgtagaaga aacaacccca gtcacattat ttttaggcga
     8101 agatgaacag agagtgttat ctgaacgtgt ggctaaactt ttgccaaaaa tttgcagcaa
     8161 ttatacattg atagacacaa aagactatcc agtagaaggt atcagtgaaa aatatcgcgg
     8221 acgtgttttg tctttcttat tgatgcatgt agtgacacaa cgcatcgatg ctcatgtgga
     8281 gcagttgaac tgccatccat tagagatacg ccgctactat cgtcagtttg attattagga
     8341 tagactggat aaaaagaata taaaaaataa agagagcagc gataactgct ccctttattt
     8401 ttttaaaata ttggcatcaa ttacgttata aaacacattt tttgcattat ctacagttca
     8461 acttataatc tgtttaatgg gacatgtggg tgccaaccat tttttgttat gcaatagtac
     8521 tatttagttg ttgcatggat ccgtagtaaa agtccaagta caacgagttg cgccagttta
     8581 catagcgttt tgtatccaga atgttgacta aaacaaaagt tcctatgtca accgtcaaca
     8641 gagacaatct gtccgttgca ggacctgaca gatttagaaa tgttcaatct gtctcttatg
     8701 tattttaaat caatactcac taggagaagt ttcctaacct cctaatagta atgctggtag
     8761 taacattgtc aaaactgctt cctcttagga acaagcaaag cataacgagt gtaaaaataa
     8821 aatgtagact attgtaacat atcattgatt atttcaagta aaaacgataa aagaagtaaa
     8881 caaacactta aaaacaatgg aaattagtcc caactttaag aaatgtatac tataatggaa
     8941 attatcaatt atttatttgg aggaatatcc atgaaacaaa aaatcgcagt tcttggtcct
     9001 ggatcttggg gcactgcttt agcacaaaca ttggcagaaa acgggcatga tgtccgtatt
     9061 tggggaaatg tacctgaaca gattgatgaa atcaataagt atcatacaaa tcagcatttt
     9121 ttacctgatt tgacgatccc agaatcgatt atcggatata aagaattggc agaagcagtg
     9181 gataacgcag atgctatttt atttgttgtt ccaacaaaag cgattcgttc agtcgcacag
     9241 gaacttattg gtaaaatgaa tactaagcca atcattatac atgcaagtaa aggactagaa
     9301 caagatacgc ataaacgaat ctcagaagtt ttggctgaag aaattccaga agaaaaaaga
     9361 caggcaattg ttgtattatc tggtcctagc catgctgagg aagtggcagt acatgatatc
     9421 acaacaatta cagctgcaag tattgatcaa aaagcagcag cctatgtaca aaaactgttc
     9481 atgaatgatt atttcaggat ttatacaaat aatgatgtga ttggcgtaga aactggtgca
     9541 gcactgaaaa atatcattgc aattggtgcc ggcgctatcc atggattagg attcggagat
     9601 gatgcaaaag cagcaatcat gactcgagga ttggcagaaa tcagccgtct tggtgtcgcg
     9661 atgggtgcca atcctctgac atttatcggt ttgagtggtg tgggggactt gatcgttact
     9721 tgtacaagtg ttcattctcg caattggcgt gccggtaact tgttaggaca gggacataag
     9781 ctggaagaag ttctagaaaa catgggaatg gtcgttgagg gtgtggcaac gacaaaagca
     9841 gcagtagagc tagctcaaca gctaggtgtt gagatgccaa ttactcaaac aatctataat
     9901 gttttgtata atggagaaga tatcaaaaaa gcagcaaaag aaatcatgtt acgtgacgga
     9961 aaaatggaga atgaattttc tcttcgttaa atagatgatc aatagttaat cgt
//
LOCUS       DACECZ010000089         9921 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.089, whole genome shotgun sequence.
ACCESSION   DACECZ010000089 DACECZ010000000
VERSION     DACECZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.089"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(144..2264)
                     /locus_tag="IWO35_002071"
     CDS             complement(144..2264)
                     /locus_tag="IWO35_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0317122.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2494..3672
                     /locus_tag="IWO35_002072"
     CDS             2494..3672
                     /locus_tag="IWO35_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ0317123.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3688..4446
                     /gene="aroD"
                     /locus_tag="IWO35_002073"
     CDS             3688..4446
                     /gene="aroD"
                     /locus_tag="IWO35_002073"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ0317124.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4469..4954
                     /gene="ybaK"
                     /locus_tag="IWO35_002074"
     CDS             4469..4954
                     /gene="ybaK"
                     /locus_tag="IWO35_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ0317125.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(5025..6278)
                     /locus_tag="IWO35_002075"
     CDS             complement(5025..6278)
                     /locus_tag="IWO35_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0317126.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVYLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6395..7744
                     /gene="gdhA"
                     /locus_tag="IWO35_002076"
     CDS             6395..7744
                     /gene="gdhA"
                     /locus_tag="IWO35_002076"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ0317127.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7856..9202
                     /locus_tag="IWO35_002077"
     CDS             7856..9202
                     /locus_tag="IWO35_002077"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ0317128.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9510..9896)
                     /locus_tag="IWO35_002078"
     CDS             complement(9510..9896)
                     /locus_tag="IWO35_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0317129.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3168 a         1800 c         2033 g         2920 t
ORIGIN      
        1 ccagaaaata ttttgtaaaa agcaagaggt tgtgaagaag ctgccctatt ctattaccgt
       61 agatgcaagc ttttgaacac tgtttattcg aaaagaggag cagcgacaat aattttgtcg
      121 ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc gtgaagttct
      181 tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc actaaagaca
      241 ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa gccattcttg
      301 tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa ccgaagaaga
      361 tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc tttcagtgaa
      421 tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt ttcgttacta
      481 tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc acggaaagaa
      541 acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata attttttgta
      601 tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc gtaagtgtga
      661 ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg ctgcatttgt
      721 gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc tagattttta
      781 tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga attatcatac
      841 aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa ataattggct
      901 gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa tcctgcttcg
      961 tcattggtga attttgaata aggataatgg ttagatacag caatgaattt tgaataaaat
     1021 ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt atcatgcaac
     1081 ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa gaaataatca
     1141 tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt cccatggaat
     1201 accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg gaaagtattt
     1261 tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga attttccatc
     1321 agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt gtcaaaactg
     1381 aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg ctggagacca
     1441 ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa atggatataa
     1501 atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc ataatgttct
     1561 tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg cacttggttt
     1621 gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag atatttgacc
     1681 aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc cgccataaaa
     1741 agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat tcttgctcgg
     1801 atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa gataataaaa
     1861 tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc tcccagacca
     1921 ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt gaattctcga
     1981 taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac catcatcgtg
     2041 atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa caatgtagtt
     2101 gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc gagatggaaa
     2161 tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa agaaaaaaat
     2221 ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat cttcctttct
     2281 gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt attctcattt
     2341 attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct gtttcattaa
     2401 ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt tttttgttat
     2461 acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa aaaaagagca
     2521 agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc tcatccccaa
     2581 aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat cgcaaaagga
     2641 tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa catcccattt
     2701 gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac tttctttttt
     2761 tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg gatcggcggt
     2821 ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga aacgttatat
     2881 gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat cataggaggc
     2941 tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat ttacggaaaa
     3001 gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt ttatttaaat
     3061 gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc attagcagaa
     3121 ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc cttttctgtt
     3181 gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa acgcagttta
     3241 aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca aaaaatcatt
     3301 gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt atatgacatg
     3361 attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg cgtagcaaaa
     3421 gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg gatacttatt
     3481 gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat tgaagaggaa
     3541 ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc aaaagatttc
     3601 cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta tcaaattaaa
     3661 aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa tataggaagc
     3721 ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt attacgtgaa
     3781 gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga tcattatcgt
     3841 gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga gatgcttgat
     3901 ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca agacatatcg
     3961 atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt agatatggtc
     4021 gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga aatcaagtct
     4081 ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc agaccctgtc
     4141 ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa actggcagta
     4201 actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg ggcaaatgct
     4261 tttgtctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat ttctcgtctg
     4321 gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga ttctacttct
     4381 gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt aaatgaaccg
     4441 ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa aaacgaatgc
     4501 aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg agtgggacga
     4561 aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat ctcgtatatt
     4621 taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga taccgggaga
     4681 agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag tagaaatgct
     4741 ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct cgcctatagg
     4801 tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg aacaaatcat
     4861 tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata tctgtgcatt
     4921 aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag aaagaaggag
     4981 gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc tctttgcccg
     5041 gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa gccgccagta
     5101 taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat tttactctcc
     5161 aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa tgatttctca
     5221 ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac aaaatcttga
     5281 atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg ctcaagaaag
     5341 aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt ctttttggtt
     5401 gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt agggaaacaa
     5461 tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac aaaacttgaa
     5521 caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc catcgtatgt
     5581 tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg taaagaaagc
     5641 ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga ctctgtcgca
     5701 taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc tccagtagaa
     5761 ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc atggaactct
     5821 tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt ctctactcga
     5881 gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc ttcgataatc
     5941 gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt taaatatacg
     6001 ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa cgtactatat
     6061 aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg attgttcgta
     6121 acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt gtttttttcg
     6181 atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat cccgcttgtt
     6241 ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca aggaaagtgt
     6301 agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga aaatgagcta
     6361 aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag aatatgttca
     6421 agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt tacaagcaat
     6481 cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat atatcgaaaa
     6541 gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag tgccttggca
     6601 agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca actcagctat
     6661 cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta tcttgaaatt
     6721 tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg gtggaggaaa
     6781 aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc gtttttgtca
     6841 aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc ctgcgggaga
     6901 tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac gattgaaaaa
     6961 atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta agatacgtac
     7021 ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg aagaaaaaga
     7081 ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca tttatgcaat
     7141 cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta gcgggtacat
     7201 ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa tcaatagaca
     7261 acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg gagaatctgt
     7321 ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa atgaaataga
     7381 cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag aaggcgctaa
     7441 tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt ggtattgtcc
     7501 aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga gtcagaacgt
     7561 ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa tcatggaaac
     7621 gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa atctgcttct
     7681 tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag aaggtttagt
     7741 ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc ttgaaaaata
     7801 ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag aacaaatggc
     7861 acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag agttagggta
     7921 catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg gcgcaggaag
     7981 cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat ttgcacgtat
     8041 caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta tcggtatcgg
     8101 tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat tcttcaattt
     8161 attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt caatcagctc
     8221 aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag taaacgttat
     8281 ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca aagagcttct
     8341 tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa cagataaagc
     8401 aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg tgattcctga
     8461 tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa tcgctgttag
     8521 tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag catactcaag
     8581 cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca ttctttaccg
     8641 taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat acttctcaga
     8701 atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca tctatccttc
     8761 aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag aaggacgtcg
     8821 caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca caattcctga
     8881 acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg acttcgtaaa
     8941 tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc ctaacttgct
     9001 agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact tctttgaaat
     9061 tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac caggtgtaga
     9121 agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag atcttgctaa
     9181 agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata atcagtgata
     9241 gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa catactgcat
     9301 cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg tttgtataga
     9361 aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag tgaggctgac
     9421 ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa gcactcactt
     9481 cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc ttttacagat
     9541 gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc gcttgctttc
     9601 aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc agaagtaatg
     9661 acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac gttgtattta
     9721 aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc aggcattgga
     9781 gctttgacta ccgcataata atctttacct acataagtag actgatagta gtcccaacct
     9841 ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt tttcatgaaa
     9901 gatctccttt tttattttta t
//
LOCUS       DACECZ010000090         9805 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.090, whole genome shotgun sequence.
ACCESSION   DACECZ010000090 DACECZ010000000
VERSION     DACECZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.090"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            119..3397
                     /locus_tag="IWO35_002079"
     CDS             119..3397
                     /locus_tag="IWO35_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ0317130.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3938..5845)
                     /locus_tag="IWO35_002080"
     CDS             complement(3938..5845)
                     /locus_tag="IWO35_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ0317131.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5975..6850
                     /locus_tag="IWO35_002081"
     CDS             5975..6850
                     /locus_tag="IWO35_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ0317132.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6930..7904
                     /locus_tag="IWO35_002082"
     CDS             6930..7904
                     /locus_tag="IWO35_002082"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0317133.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8093..9229
                     /locus_tag="IWO35_002083"
     CDS             8093..9229
                     /locus_tag="IWO35_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0317134.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9366..9710
                     /locus_tag="IWO35_002084"
     CDS             9366..9710
                     /locus_tag="IWO35_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ0317135.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT         3192 a         1805 c         2063 g         2745 t
ORIGIN      
        1 ttatattttt tctttttata aaatacactt attataattt tgttttttat tgacagtaaa
       61 aataaattag gtaatacttt aagtaaacgc tttctaattt gttacgggag gaatacactt
      121 gttaaaaaaa cgtataaaat acagtgcgct aatgatgaat ctccttatgc tcagtacccc
      181 ggttttaatt tctgtcgttg aagttgctgc taacgaacaa cagtctgtga acgaagaaaa
      241 tgcacacgtg aacattgaag ggatgtcaac aatggaggaa ataccaagaa ctagtgaaga
      301 tatggatacg gaagaaggaa atgatcctat gatgaaagaa tcagaaaatg aaaagacaaa
      361 aaagcaatct gaagaagcaa atgaagagtg gaaaggggtt gttttcggag aaagcacaag
      421 tgctagtagg tatgccattg aaccattaga agacggcgtg cggctctctt ccacgaataa
      481 cggcggaaaa ttccagtcaa gcggatcaga tggaatgacc tattattatc aagcaatccc
      541 caaagacatc aactttagga tgcgggcgac gatcgaaata gaatcttgga cctatacgaa
      601 tgcccaagaa ggatttgctt taatggtaag agacgcagta ccaaaaaatc atttgtttgg
      661 gcaggcattt tattcaaatt catttgccat actaggcagt cggatcgaat atgtctggga
      721 ctcggataga caagaagtgg tcaacacgag cggcagcaaa tatacgatgc gacttggatt
      781 ggggacacgg gcaatcacag gaatatcatc tgaagatccg caagctgcgc cagctcctgg
      841 gagtgtctca gtggaaacaa cacctttaga gacttcagca gggttcttgg aaaaagaaac
      901 aggttcttat aatatattcg gaaacggtca tgggaatctg tttccggcca cgaactcgat
      961 tactaaactg gacgtagaga tgaaaaaaac gaatactggt ttagaggcat attattacga
     1021 tcctgaaaca ggagaagaaa tggcttctga catcatgtat gactgggaaa aattatacgc
     1081 ttcagatgaa tcggttattt atgcaggatt tgctgcagca aggaatatga cgatcaaggt
     1141 agaaggaatc gaagttgctt actcagatcc tgcgacagat cctcctgggc aagaaagacc
     1201 tactcgcttg attgatccga tttacactgt gacctctagt aatcattcag gaaaccaaga
     1261 tcatactatg tcctttagag caaatgctga cggattattg acaattaaaa ataaagcaac
     1321 aggagaggtg cttaagaacg caaaaaactt ggctatcact gcgaatcggg aatttgatta
     1381 tcagacaaaa ctgatggaag gaacgaacga atttactttc agttttacgc cagataaaaa
     1441 ctatccgcca gaagaatacg tagagatgac tagttatgaa acaaaagaga ttcttcatac
     1501 agtggactac cgtaaaccta aatattcgac agtttatgta acaccagaag ggtcagatgc
     1561 aggaaacgga agcagacaaa atccattatc tctgacacaa gcattgagat acgcatatgc
     1621 tgggcagaca atcgtcatgg cgcctggaac ttattccttc gaaagaggat tgacgattcc
     1681 aaaaggcgtt aacgggacaa gagaaaaccc gattcagttg attgcagaga aaaatccaga
     1741 aaacaagcgt cctgtacttg attttaaagg aactggaaat gggatgacac tgttcagtca
     1801 ctactggggt ttacgagggt ttgacattac aaatagtgca gctatgcaaa aaggattaca
     1861 gatttctgga aatcataatt tgatcgaaga gattcatgct tatcggaatg gaaatacagg
     1921 aatccaaatc agcggtagct caaatgatcc tttcgaagca tggcccgcgc ataacttagt
     1981 caagaactgt acatcttttg aaaatgccga tccaggattt gaagatgccg atggatttgc
     2041 ggccaaactg acaataggag aaggaaatgt atttgacggt tgtatcgcgt atcataatgc
     2101 agatgacggt tgggaccttt ttgctaaaaa tgaaacaggt tcgatcggaa aagtaatcat
     2161 caaaaactca gttgcttatc gaaacggctg ggtaccagga attgagggag aaggtaacgg
     2221 taatggattc aaaatgggcg gttcttcttt aacgggccct catgaattga tcaatagttt
     2281 gtctttcgaa aatcttgcga aaggaatcga ttccaatagc ggaacagata ttatcgtcaa
     2341 tagcagtaca agttttaata acggttcata taatgtggct ctttatacct cttcagcagg
     2401 aaacacagac taccatgctt ctggtatctt atctttccga acagagaatc ttgagatgag
     2461 agagcagatc cgcccattga atcaaaacga agatcaaatc taccactcgt tgaactacta
     2521 ttggaatgaa cgagagaaac aatcagaaaa tactcttgga cataccatat caaaagattg
     2581 gatcgagtct ctaaatacat gttcgaaaga aggtcagcag cctgttacgc gctatgaaaa
     2641 tggaagtatc catgttcatg gactgatgca gattaaaccg gggaatcgtc aaacagaaaa
     2701 ggaaagagtg gatggtcttc cattatctgt aggtgctatt tttgacggag aaacctcacc
     2761 gtcttcggaa tatccagagt atgcaatcgt acaagaacca aatcagacaa aaccaaatga
     2821 acaaaataat ccgattatta tacaagaagc aaatggtgtg gcggagaatg cagaaggaaa
     2881 tatcattgaa ctgaagcctt ttacagtttt tgtgtcaact ttcacaccaa atcaaggaac
     2941 gcctgtcttt gaagatatgc gttatttccc agaaaagatc caaatcgttg aaacacagtc
     3001 aagtgctcaa ttttctaatc ataccgctat tttttcagca ccttcaaaag gagaatatac
     3061 gctagaagtc acttatcgtt cggaaatatt tgatggggaa aaatggtctg ctcatacaga
     3121 atctgttacc cagcaaaaag cgatcactgt gcgagaaact gccgatgtgc ccccgggaaa
     3181 tgacaaacca gaaagagaaa atccaggaaa tgaatcacca aatggaaaac caaatggaaa
     3241 accttctcat ccggatagga cagcaaaaga aagtgaaaaa aatggcactc aaggactccc
     3301 aaaaacggga gaatttaata acccgttgct agcattagct ggaggaattt tgttaatcgg
     3361 tgtagttgtt tatgtcatga aacagcgaaa aaaataagtg gaatttctcc ttttatgatg
     3421 taaaacaatt gactgaggaa agtaaaaata gaaggaaatg agaccaatag atgtctcact
     3481 tccttctatc tttttataga tgaggttctc cttgctgctt ttgattggct gttcttatta
     3541 cttgatgcag gacagctttt tcacaatctc tccactaaac ggtgttcaat cagctgcccc
     3601 tcggtattag ctcaacaaac ggcaaaatat gaggaactat ttttccgttt ttttctctaa
     3661 tactcaggtc aggacgctga tttcacaacc tctcgataaa cggtgttcaa aagccagttt
     3721 ctcggtagta agtcaaattt atgcaaaaat gtggaaagcc attttcacaa aatttttctt
     3781 actacttgaa actaagcagc tttttcacaa cctctaatac atggtattcc agaagtaact
     3841 tcttcgaaaa ataagccgaa atgtcacaaa aatttgagaa gcgattttcc caaattcctt
     3901 cttatttctt gaagctgacc acttctggca caacctctta ccagattcgc actctttttt
     3961 ctggcgctag atacatagcg tcttgttccg tgataccaaa tgtttcatag aattcgtcaa
     4021 ggttttttgg ttgcacgttt gctcggagtt tagcaggggc atggacatcg atttgcagta
     4081 aaagttgttg gtattctttt tttgcttttg tacgccaaat cgttgcccaa ttgacaaaga
     4141 attcctcgat agaaagatct gattccgttt ttgctgcttc caatgcacag cttagaccgc
     4201 ctgcatcagc aatattttcc gaaacggtta attttccgtt tacttttccg tcagcaaaag
     4261 gcagaccatc gaattcttcg atcattgctt gtgctttgtt ttgaaattgt gtaagatctt
     4321 cttctgtcca ccagttgttc aagttgccat attcgtcaaa taaagcgccg ttattatcaa
     4381 atgcatggga aatctcatga gcgataacag ctccgattcc accataatta gcagagcttg
     4441 attgttctag actgtaaaat ggtgcttgaa ggatcgctgc cggaaagacg atcacattcc
     4501 gaaatggatg ataataagca ttgaccgtgt cagcactcat ttcccattct gaacgatcca
     4561 ctggtttgtt ccatttgctg aaacgatctt ttaatgtgag tttgctgaaa acaagagtat
     4621 ttgaaagcaa agtaccaccc tgatctgcag gtactgtttt gaactggtcg aataatgctg
     4681 ggatcttatc ggggtagcct acttggatac ctaagttatt taattttatg acggcttttt
     4741 cttttgttgc atccccaagc cagtcatttt cttctagacg ttttttataa acggcgatca
     4801 tttttttcac catttgttct acgtcttttt tagcagcttc gccaaaatat ttttttccgt
     4861 agtaatcgcc aactacttga tcgaattgtc cagaggcaag gtaatacgct gcttttttct
     4921 gaggcattgc ttcgtctgtt ccagacaata cgcgagaata gattccgctg acttggcgga
     4981 attcttctga tagataacca ctaagagaac gtactgtttt aatgatcatc cagcttttga
     5041 ataacggaaa atgagcttct gtcaggatgt cattcaaatg atcgaagtac actggatcag
     5101 ttacgatcac tttatctgga gttgtcccaa tcaaacccgt gacaagtttt ttcaagtcca
     5161 gtttatctgt gtatttaata aatgtatcaa aagattgcgg attatacatt ttgctgtaat
     5221 ctgcgttttc ttctgcactt tttacatgag gagcaatcaa tttatcaaat tgcactgctt
     5281 gttccgtgat tgcttctgcc tgactttttt ctttgccggc aagctctaat aactgaacag
     5341 ccatatcaaa gaaaacgcct attagttgtg caccgttggg atggtctgat gcataatacg
     5401 tcttatctgg aaggaataaa gaaggcggat aagcaaagaa ggcgttcgtt tgcgcattct
     5461 tcatatctgc atctacatcc agattaaatg gcaatggtaa tgtatccaac agccattctg
     5521 gaaattgctt attcaattcg tcgaaagact gcaatgattc aactttttcg attataggtt
     5581 gaagcggttg agcatgcaac tcatttcttc ggtcataatc gtttgccagt tgataatatg
     5641 caaggaaatg tttcattttt tctgaagaca ctttttctgg atgggcaagc agatcgtctg
     5701 tttctcccat caaaagttgg tcgatcccgt caactaaatc ttggaaaccg cctgtagctg
     5761 gtttatctgc agggattttt gctgtttttt gccattcttc attgactgct tcatagaaat
     5821 cttgtttcaa tcgttctttg ttcattcatc gatcgctcct tttttgtttc gtgtgcccat
     5881 tatcttacaa aatgaaggaa ttaacaaata gaacataaaa aattgaaaat tcgaacaatc
     5941 aaactgattt gtttttttca atccttcact tataatgaaa aaaaggaggc gatcccaact
     6001 gaatcaagta gtggataaac ctttatattt caaagtagat aaaaaaatta aaaaattagc
     6061 cagcactcag cagctgcagt caagaaaaag taaattgctt tttttagctc tggtttttga
     6121 agatcaaagt tatgtgatta tcgatcaatc aggacatccg gtagaatatt ctccagcaaa
     6181 atatacttat caagagggtc tttcttgtaa aaaatggaag ctgatcaatg aagagccaat
     6241 cgagttatct agatggatca acagaaaaga agatattccc gttttgatcg aagaaaaaag
     6301 gtcaggaaag gaactcgcaa attgctgggt gggattgcca gaagaacgtt ttttgaggta
     6361 taagaaatgg gctaccccct caggctatct ttgtggtact tacgcggcag cagttctcct
     6421 tgcctattat caagactatc gtaaggggtg gatgcttcca aatgagattc gcaagaaaaa
     6481 tacggctgat tcgttggttt tgacgaaggc attgcgtagc cagatccaac cactaggact
     6541 tcctacgatt ccttttcagg ttagtacagg gatttctagt tttttaaaga agaatggcaa
     6601 tcatgagcgt gcacgagcaa ctcttctagg ttcatggcag cgagctacaa aacgtatacg
     6661 tgaaggcaaa cctgtgatga ttggtatttt gaaagtactt ggcagtacat atggcaatca
     6721 ttgggtgaca gcgtacgctt attttgaaac tgaaacagga gaacgttatt acaaagtaca
     6781 cgacaactgg ggcgattatc ataaagtcat tcctgccagt tggagtaacg gaacagtttc
     6841 acttccttaa gaatttgctg aaattaacag aaaatcttgg aaaaatatgt taaactatcg
     6901 aagaatagag aagttttggg gaggactaca tgagtaataa gtatcaggat gacacgttga
     6961 agatttttag tttgaatggc aaccgtccat tagctgaaaa aatcgcgaaa gtgttcggaa
     7021 cagaattagg taaaagcgtc gtgaagcaat ttagtgatgg cgagatttcc atcaatatcg
     7081 aagaaagtat tcgaggggat catgtgtata tcgttcaatc aacgaatgca cctgtcaatg
     7141 attactatat ggaactactg atcatgatcg atgcaatgaa gcgagcaagt gcgaaaacga
     7201 tcaatgtcgt attgccttac tatgggtatg ctaggcaaga ccgcacggcg aaacctcatg
     7261 aaccgatcac tgcgaaattg atcgctaact tgatcgaaga agcaggggca actcgagtac
     7321 tgactttaga tctgcacacg gttcaagtac aaggattctt tgatattcct gtagataatt
     7381 tatttacgat gccgttgttt gctcattatt accgtcaatt aggtctgacg ggagatgaca
     7441 tcgtagttgt ttcgccaaaa aacagcggtg ttcagcgtgc acgtagtctt tcagaatatt
     7501 tgaacagcac attagccatc gtagatcatg cagatgatgc ttcaactgag ggtggttatg
     7561 tgatcggtga tgttcaagga aagacatgta tcatggtaga tgatatcttg aacacaggag
     7621 caacgcttgc acgagcagca aatgtattaa aagaaaacgg cgcaaaagaa gtatatgcct
     7681 gtgcgtcaca tggattattg tccgatcatg cgaaaagtat tttagacaat gcaccaatca
     7741 aagatatttg tatcactgat tctgttttca cagaagaaaa tcgtcatcca gaaaacttaa
     7801 caatcgttac atgttcagaa ttaatgggtg aagcggtcaa acgcatccat gaaaatacac
     7861 cgatgagtcc gttattccgt ctggaagaaa aagaattcga ataaacaaaa aaaagaggga
     7921 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
     7981 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
     8041 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
     8101 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
     8161 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
     8221 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
     8281 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
     8341 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
     8401 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
     8461 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
     8521 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
     8581 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
     8641 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
     8701 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
     8761 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
     8821 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
     8881 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     8941 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     9001 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     9061 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttagaagcaa tgtatggaga
     9121 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     9181 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     9241 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     9301 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     9361 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     9421 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     9481 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     9541 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     9601 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     9661 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     9721 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     9781 gcaaattagt tgtctttttt atttt
//
LOCUS       DACECZ010000091         9735 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.091, whole genome shotgun sequence.
ACCESSION   DACECZ010000091 DACECZ010000000
VERSION     DACECZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.091"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            332..1381
                     /locus_tag="IWO35_002085"
     CDS             332..1381
                     /locus_tag="IWO35_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0317136.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            1378..2259
                     /locus_tag="IWO35_002086"
     CDS             1378..2259
                     /locus_tag="IWO35_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0317137.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            2262..3032
                     /locus_tag="IWO35_002087"
     CDS             2262..3032
                     /locus_tag="IWO35_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0317138.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            3311..4165
                     /gene="aroE"
                     /locus_tag="IWO35_002088"
     CDS             3311..4165
                     /gene="aroE"
                     /locus_tag="IWO35_002088"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ0317139.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            4343..5506
                     /locus_tag="IWO35_002089"
     CDS             4343..5506
                     /locus_tag="IWO35_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ0317140.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            5623..6033
                     /locus_tag="IWO35_002090"
     CDS             5623..6033
                     /locus_tag="IWO35_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0317141.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6207..8435
                     /gene="topB"
                     /locus_tag="IWO35_002091"
     CDS             6207..8435
                     /gene="topB"
                     /locus_tag="IWO35_002091"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0317142.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            8573..8890
                     /locus_tag="IWO35_002092"
     CDS             8573..8890
                     /locus_tag="IWO35_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317143.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9050..9286
                     /locus_tag="IWO35_002093"
     CDS             9050..9286
                     /locus_tag="IWO35_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0317144.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            9283..9681
                     /locus_tag="IWO35_002094"
     CDS             9283..9681
                     /locus_tag="IWO35_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ0317145.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3384 a         1651 c         2069 g         2631 t
ORIGIN      
        1 aaaaattcga ttagacttaa atcaattctt aaggaaaggg tgacctgtaa tgaacttatc
       61 aaaacgtatt tcagcagaaa acaaaaaaat tggcgcagct ggcgtagata agttgtatta
      121 atggtgagac tttcccatag atacaacttt ttagagtgca tttctaaagc ttgtatctat
      181 gctagacatg aacattgact gccgcataga ttagaatcta tgcggttttt tgtgtaaaga
      241 caatgaccgc tttggccatt gtcttttttt gtagactgga tacttgcctg attataataa
      301 ataaaaagat tgactttgca ggaggaaaaa aatgaaaaac aaacgattaa taacagttgt
      361 agtaattatt ttagtttatt tagcaggtgc atttatttat ggaaaagcct caggcgaaaa
      421 agaaagtact tcgacgaacc agacaaaaaa aacaacagtt gttggggtat tgcaatacgt
      481 gagccatccg gcattagatg agatttatcg aggaatcaaa gatggtctga aagaagaagg
      541 attaaaagaa ggagaaaatc ttgagatcaa atttcaaaat ggacaagccg atcagagcaa
      601 gctagccaca atgagtcaac agctagtaca atctgatcca gatgccttga ttggaatcgc
      661 aacacctgcc gctcaatcat tagcaaacac gacttcatcg atcccagttg tgttaggtgc
      721 tgtaacggat cctgtggggg ctggattagt cgcttcttta gagaaacctg gtggtaatgt
      781 aacaggtgtt tctgatcagc cgcctgttac ttcacaaatc aagcttgctt ctgatttgtt
      841 gccaaatgca aaaaaagtcg gaatcttgta tgcttcttca gaggataatt caaaatacca
      901 agtagcacaa gcagaagaag ctgcaaaaaa agaagggtta gaatcagtaa aatatgcggt
      961 gccttccact aatgagattg cacaaacggt tcaagtgatg agccgacaag tcgattttat
     1021 ctatgttcca ttagataata ccattgccaa tgcgatgcaa acagtggtaa aagaagcaaa
     1081 caaagcaaat atccccgtca ttccatctgt ggatacaatg gtggaacaag gtggactagc
     1141 aacaatcggg atcaatcaat atcaattagg gcttcaaagc ggtaagatgg cggcagaact
     1201 tgcgagtgga aaagaaaaac cggaaacgac gcctgtttat acatttgatc aaggggatac
     1261 agtgatcaat caatctcaag ctgatcgctt aggaatcatt gttccccaat cgatgaagga
     1321 aaaagccaaa atcattacag atgactcgca gcaagaaaca tctaaggagg atgacaaatg
     1381 atcgtatcag ctatttcaca aggcatgctt tgggcaatat taggattagg gattttcatg
     1441 acttatcgga tcttagactt tcctgatatg acgacagaag ggtccttccc attaggtgct
     1501 gctgtctgtg tgacagctat tactcatggg gtagcgccga ttgtagctac attattaggc
     1561 gtaggtgctg gcatgatagc aggactcgtt acggggttac tttatacaaa aggaaagata
     1621 ccggttattt tagcagggat tttagtgatg tcaggattga actcagttat tttgtttgtg
     1681 atgcgaacac caaatctttc tttattgaat catccgattc tacaagacag cttccatcgc
     1741 tttgggttac ctgattactt tgacacagtc cttcttggct tcatctcttt gggcatcttg
     1801 attgctgtac tgctattctt cttcaataca gatttgggtc aaggctatat tgcgacagga
     1861 gacaacgaaa cgatggctcg gtcgttaggg ataaaaaccg atcgtatgaa aattttaggt
     1921 ttaactttgt cgaacggtgt cattgcatta tccggtgcgc tgattgccca gaacgacgga
     1981 tatgcagatg taaataaagg gattggcgta atcgtcatcg gtcttgcttc catcatcatc
     2041 ggtgaagtga tcttccaaga attgacgcta gcagaaagat tgattgctat tgttgtcggc
     2101 agtatcattt atcagctatt gattttagta gtaatcaaac tagggtttga cacgacttat
     2161 ctgaagttgt tctccgctat catcttagct atttgtctga tgatcccgca attgaagaaa
     2221 gcactgaact taaaaacggg ttttgagaag gaggcatagt aatgactgat atattgacaa
     2281 tcaaaaatgg cagaaaagtc gtaaacaatg gaatgaatga agagaaagta ctattggatg
     2341 aaattaacct tacaatcaga gaaggggact tcatcactgt ccttggcggg aatggtgctg
     2401 gaaaaagcac actgttcaat acgatcgctg gtacgcttca actcagtcaa ggatcgatct
     2461 cttttaaaga tcagctgatc acaaaagatt cagaagaaaa aagagcacga ttcctttctc
     2521 gagttttcca agaccccaag atgggaacgg ctcctcggat gacagtagca gaaaatttgc
     2581 tacttgcaca aaaaagaggt caaaaacgga aattccgttt acgtaacttg aaaaatcaaa
     2641 aagaagattt tttcagacta tgtcaagaag tcggtaatgg cctagagcaa cacttggata
     2701 ctccagcggg tgaattatct ggtggtcaac gccaagcatt aagcttgctg atggcgacga
     2761 tcacagctcc tgagttacta ttattagatg aacatactgc agctttagat cctaaaacag
     2821 ctaaagcatt aatgcgatta acaaatcagc ggatcaatga acaaaaattg acttgtctca
     2881 tgatcacgca tcgaatggaa gacgccttga aatacggaaa tcgtctgatc gtactccaaa
     2941 aagggaaaat cgttcaagat atttctgaaa aagaaaagca acatttgaca atgggcgacc
     3001 ttttgcagtt ctttgaagaa gtagtggatt gatttcgaca gaaaaattcc tttcagtaga
     3061 aggacttgaa aactcattga catcttcctt tgattcatcc tatacttaaa acaataaaca
     3121 attaaacata tttgggtgtt ttgtaggatc acgtagtcaa tgattttaac aattaaatag
     3181 aatattcttt gctctgacaa gtaaacgatg agaaaagaat ttgctacttt gccacctaga
     3241 cgtttcgtac tttttcgtat caaacgctaa gtggcttttt ttattgaagt aagaacaaag
     3301 gaggaataag atgatttctg gacaaaccaa attagctggg ctctttgctg atccggcttc
     3361 tcacagttta tccccattga tgcacaatac tgcttttgaa gcaaacaaca tcgatgcggt
     3421 gtatttagct ttccaagtaa atccaatgaa tttgaagcaa gcagtcgaaa gtattcgaac
     3481 ttttgatatg ttaggtgtaa atctttcgat gccgaataaa acagcggtta ttccttatct
     3541 ggatgaggta agccaggaag cgcaattgat cggagcagtg aatacgatcg tgcacgaaaa
     3601 gggtcgtttg attggctata atactgatgg tgcgggcttc atgcaatccg tgcaagaagc
     3661 tgggatttcg atcaaaaaga aaaaaatgac tctactggga gctggaggtg cagcaaaggc
     3721 agtcactgtt caagcagcgt tagacggcgc tgaggaaatc gtagtttata aacgaaacaa
     3781 ccaaacattc gctcaagtaa aagaaacatt cgaacaactc agctatcgaa cttcttgtaa
     3841 gataaaagtt cgtgactttg ccgatacgca tcaattaaga aaagacttga aagaaagctg
     3901 tcttctagtc aatgcaacag atatcgggat gggaaagaaa atcaatcagt caccgctctt
     3961 agatataagc atgatgcatg agaatctagc ggtttttgat ttgatttatg cacctagaga
     4021 aacacgttta ttaaaagaag caaaaaaagt aggagcaaaa ggatacaatg gacttggcat
     4081 gttgatctac caaggagcaa tcgcgtttga attatggaca aaggaaaaga tgccgatcca
     4141 tgtgattgaa aagctgtttg attaaaaaag gataatagat aaagatcttt tattctcgca
     4201 taaacggtct caagaaaagt aatcctttat gtatcgaagc atttatttcg ttatttttta
     4261 ttaaattatc aaaaaatgat aggcttttgt cgattggtta tcgtatcctt tatttatcag
     4321 taagaaacga ggaggaacca caatgagtcg gatggatcgc tataaggaca tccataagaa
     4381 ggcgaaacct ctgaagcggg atacggggag tatttttgct agaagagaac gaaaacgaac
     4441 gcgtatggaa aatgaggaag atgctacccg tatttatgaa agagagcagc attcatttga
     4501 acatttgaac caatacgagc aacagatata cagagatgca ccaagagcag aagaacaaca
     4561 aaaaaataaa aaatttttca atcggcaaaa gaagaacaaa ccgttaaaac ctcgaagaaa
     4621 gcgatcgtgg aaaaaaatca ttctatcgat tttgcttgtt ttgatccttt attcaggtat
     4681 ctcatttttt attggtaaat atgtagctga acatgacaca agcttagagg cagcgacttc
     4741 agaaaccttt catgggcaaa aagcgacgaa tggtgcgcat aatattttga tccttggaag
     4801 tgatacacgc ggagaagatg ccgggcgagc cgatacgatc atggtcttac agttggatgg
     4861 tccttcccat aagccgaaac tgatttcttt tatgcgtgac agcttcgtta cgatcccagg
     4921 ggtaggacaa aataagatca attcagctta tgcatacgga ggagccgatc tagtgcgtca
     4981 aactttagta gaaaattttg gaatcgattg tcaatactat gcgaaagtcg atttcaaatc
     5041 gtttgaaaaa gtgatcgatg ccttattcat gaacggagtg aagatcgatg cagaaaaaga
     5101 tctgaatctg gatggtgtag atattaaaaa agggggccag aagatggatg gacatgttct
     5161 tttgcagtac gcacgtttcc gaatggatga acaaggagac tttggccgtg tcagacgcca
     5221 acagcaagta atgaacgcta ttttcagcca attgaagaat ccattgaatc tgatccgtgc
     5281 accttatgca gctggtaaag ctttaggata tacatccaca gatgtcactg cttggttctt
     5341 gacgaaaaac ctcttttcga ttgcacgagg agctggtggc attgatcgaa taagtgtgcc
     5401 agtagacggt tcgtggaatt atgggcaaag tgattatgct ggcagtattc ttgaaattga
     5461 taatcaaatg aatcaatctg ctattacaga ttttttgaat aaatgatcat aaaaaagatc
     5521 aagaagaagc tgcgcaaggc ttttgtcatg aatcaatcaa acgattcact cgtaaaaaac
     5581 gctttcttat ggtagattta accaagagag gagctgttta cattggatat ctatctgaac
     5641 ttcaaaaatg agtcgcgtga agccatcgct ttttatgaaa aagtttttca gactgaagca
     5701 tcaggtatca ccacatacgg tgaaatgcct ggaatagaac aaatgaactt gccagaagaa
     5761 gcaaaaaatc taatcacgaa tgcacggctg aacatttatg atacattagt tatgtttgca
     5821 gatacgccag aaggaattgg tcctgaatta gttgtgggaa ataatgtttc tttagtcatc
     5881 cagacaagaa atgaagaaga aacgaagcgc ctatttgaaa gcttagctga gggtggtaag
     5941 attgagatgc cactggatgc tgttccgtgg gctcagttgt atgggatggt aactgatcga
     6001 tttggcatca actggcagat taaccaagag tgacagctaa agatgaatat tttctggaaa
     6061 catcaagcag caaacggatc atcgtttgtt tgcttgatgt ttttgtgtct atttcgacta
     6121 tacggtatct tttttttatt gccagatgcg aaaatatgtt ccataataga gaagtccgca
     6181 aaaaattaag taacggaggt cagaagatga agacggtgat actagctgaa aaaccttcac
     6241 aagcaaaagc ctatgccgaa accttccaac agcaaaagcg gcatgaaggg tattatgaaa
     6301 tcagcgatcc attgtttgct ggtgaagtaa ccatcactta tggttttgga catttggtag
     6361 atatggttgc tccaggagct tatgaagagc gttgggcaaa atggtcttta gaaaatttgc
     6421 ctattttccc agaaaccttc cgttacgaag ttccaatgga caaacgtgca caattttcga
     6481 tcgtaaaacg agaattgcag tcagccgata ccatcattat tgcaacagat ggtgatcgag
     6541 aaggtgaagc catcgcgtgg tcgattattc gacaagcaaa cgcattttct aaggaaaaga
     6601 actatttacg tttatggatc aattctcttg aaaaagaagc aatctatgaa ggttttaaga
     6661 atctccaacc tggaaagaac tattttccaa aatataaaga agcgcaagca aggcagaacg
     6721 ctgattggct gattggaatg aatggaagtc cattatatag cttgctactt caacgaaaag
     6781 ggattgatgg cagtttttct cttggtcgtg tacaaacacc gacactctat atgatttatc
     6841 agctgcaaga agaaatcaaa cacttcaaaa aagaaacgta ctttgaagga gaaggtttaa
     6901 ttacgacaaa caaagggcag ttttctggaa aaatcgtacc aaaaaaagca tttaaaaccc
     6961 aagaagagtt aacgaaggaa atagaaaaac ttggagcgca tttaggtaag caaaacggac
     7021 ggattcttga agttacgaaa aaggaaaaga ggttgcatag tccacgttta ttttcgctat
     7081 ctagtttgca gacaaagatg aatcagctaa tgaaagcaag tgccaaagct acacttgatg
     7141 ctgctcaagg attgtacgaa agcaagtttc taagctatcc aagaacggat tccttttaca
     7201 ttacagaaaa tgagcatcag tatctggtta aacatctgca aaattataaa aaatttttag
     7261 gaatagaaga agtacagaca actgagatca aagcaaagaa acgatatgtc gatgcgaaaa
     7321 aagttcaaga acatcatgca attattccga caaagacgat tccgactcct gcacgctttg
     7381 cgaagctttc caaattacag caagcaatct atttgcaagt tttgcgcact actgtggcta
     7441 tgtttgctgc agattatatc tacgaagaga cgaccgtaat gacgggcgta cagcaattaa
     7501 agctaaaaag catcggaaag atcccattac agcaaggttg gcaggaaatc ttacgcatca
     7561 agtcaaaaaa gaaagaagct cagactcttc ctcctatcgt agaaaatgaa gaagcagaag
     7621 tagaactgaa aagtatggag aaagaaacac aacctccaaa accctatacc gaaggaacgt
     7681 tgatcacagc aatgaaaact gcgggaaaaa cgctggataa tgatgaagcg caagaaattc
     7741 tcaaagaagt agaaggaata gggacagaag ctacacgagc caatatcatt gaaaatctta
     7801 agcagaagca ttacatcgaa gtcaataaaa acgaaataac tgttacgcca aaaggaatca
     7861 ctctttgtaa ggcagtagcc aatgaaccat tactgacgag cgcagaaatg actgcgcgct
     7921 gggaagggta cttgagaaaa attggtaaac aagaggggac accatctgtt tttttagaga
     7981 atatcaaaaa attcatccta catttattgg cagaagttcc taatcaaatc gacaaggtag
     8041 acttttcaga tgagattagc gggacacgtc agataaaagc gatggagaag aaaaacgatc
     8101 aattaggaag atgtccaaaa tgtaaaaagg gaatagtcat gctttatcct aaaatcgcta
     8161 catgcttgaa tccggaatgc gacttcaaat tgtggccaac agtagctaaa aagaagctga
     8221 ctaaaacgaa tctcagagat ttgctttcaa aaggaaagac aagtaaagtt gtcaaaggtt
     8281 ttactggtaa aaaagggaaa ttcgatgcag ttttagtatt aaaagaagat atgacaatcg
     8341 gtttttcttt tccatggatc gaaaacagtg gaacaaaagc agttgatgga aacaaagaaa
     8401 catcaagaaa aaataatgaa aacgaagtag agtaaatgaa aacaatgaaa tagcggaata
     8461 tttattccga aaagtgagaa tataaatacc aataaaacta aaaaatatca gtgaatcaag
     8521 caaaaacgtt ggggtaggct gattttatat agatagcaag gagttttttt atttgaaaaa
     8581 agtgcattca tttttaaatt atcttatggg agcaatcacg attgtggctg tgtaccaagc
     8641 atttctaaag tatcctgatt acgggacgaa gttgttgtca ttgacgcaca atcaaacagc
     8701 atattcacca acgttgattt tatcgacttt gactgcagga gctaaaagcg ctacttattt
     8761 ttttcataaa acgacaagca tgctaaagcg aaagaaacag cgaaaacaat tgttggtggt
     8821 ggtagcggct atgatcatta tttattttct accagttatt acacaagtat ttcatcaata
     8881 ttttggttaa gaatacgaga gtttgtgaca aaagtcatgt cacaaacttt tttctttgtc
     8941 attttttcgt aagaaaaagc aaaataaaag tatatacatt gattaaaagt atatacttgg
     9001 atataatgta cttactatag aatgagaatg gtatagaggg tggcgaaata tgaaaaaaat
     9061 gaaaaagtat aaaattagaa aatctggcaa tagcgatgta acaactatcc caccagaagt
     9121 aaaagaattc atgggtgtgc agacaggtga cgcaatcagt tatgtttttc aatctgatgg
     9181 ttctgtacga atgatcaaag cacaagaaga acctgatatt gattcattag ttgattcgat
     9241 catgaatcaa tatgaagacg cgttaaaaga tttggttgat ttatgaggta tctttcgact
     9301 aaagaaatta tcaaaatcaa cgcgaaagtt attttacgtc actcgccagg cgaaatgatc
     9361 ggtgtaaaag atgcaaatgc attagatatg gccgtcaaac agccttcaca agcagtattc
     9421 aatcaagaac tctatcctga aatctatgaa aaagcggcga ttttagcaat taatttagct
     9481 aaaaaacatc cttttcataa tggcaataaa cgaactgctc ttgttgccat gttgttgttt
     9541 cttcaattga ataattgtcc agttgctttt tctcgtcaag aagctgttga ttttatcata
     9601 atgattacca ctagttcatt ggatttcgat tcactaaagt ccaagataac cagttattta
     9661 cgacaaaccg caataaaatg aacgaattaa aaactttatt tctcaattag atcagcatgt
     9721 cattaaggaa tatgc
//
LOCUS       DACECZ010000092         9694 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.092, whole genome shotgun sequence.
ACCESSION   DACECZ010000092 DACECZ010000000
VERSION     DACECZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.092"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            116..310
                     /locus_tag="IWO35_002095"
     CDS             116..310
                     /locus_tag="IWO35_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0317146.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            313..1515
                     /locus_tag="IWO35_002096"
     CDS             313..1515
                     /locus_tag="IWO35_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0317147.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(2098..3729)
                     /locus_tag="IWO35_002097"
     CDS             complement(2098..3729)
                     /locus_tag="IWO35_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ0317148.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(3743..5416)
                     /locus_tag="IWO35_002098"
     CDS             complement(3743..5416)
                     /locus_tag="IWO35_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0317149.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(5429..5746)
                     /locus_tag="IWO35_002099"
     CDS             complement(5429..5746)
                     /locus_tag="IWO35_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0317150.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(5771..7171)
                     /gene="lacG"
                     /locus_tag="IWO35_002100"
     CDS             complement(5771..7171)
                     /gene="lacG"
                     /locus_tag="IWO35_002100"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ0317151.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            7408..8193
                     /locus_tag="IWO35_002101"
     CDS             7408..8193
                     /locus_tag="IWO35_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0317152.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8196..8888
                     /locus_tag="IWO35_002102"
     CDS             8196..8888
                     /locus_tag="IWO35_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0317153.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(9162..9533)
                     /locus_tag="IWO35_002103"
     CDS             complement(9162..9533)
                     /locus_tag="IWO35_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ0317154.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         3062 a         2056 c         1700 g         2876 t
ORIGIN      
        1 ttgggaagtc ctgaacaagt gatggatttt caagaaagta ccgttcatta tacaaaaata
       61 aagaaataac actattaccc aatgatagtg ttttctttga aagtgagttt atacaatgaa
      121 aaatacaatt tgtcgaaaag atttagaggc tctaggattt aaaacacatc aagctaccac
      181 aataatccga caagcaaaaa caagaatggt aaatcaggga tatccatatt ataataataa
      241 aagagttggg gtagtacccc gacaagtaat tgaagaaatt ataggtgttc ctctttcaca
      301 ggaggaataa ctatgaaaac aaaataccct tgtgtgtatc aagataaaaa tggtcgcttt
      361 tcttatctag ttgagttagg aacagatcaa gtgactggaa agaggatcca gaagaaagga
      421 acaaaagatt ctctaggaaa acgattctca tcagctcgtg aagcacacaa agaagtcatg
      481 cgtataaaaa atgaatacat ggttgtaaat ggatttgcga attatgattt aacttacaaa
      541 gaatttatgg agcagtctta cgttccacac tacaaagcta gcgtacaaaa aagtacttgg
      601 agttctcgtg agtgtggatt gattcagatt actgaatttt tcggtgataa gaagcttcgt
      661 gaaatcaatg tgcgagattg tgaaaattat cgaatttggt tattgaacga tagcggatac
      721 tcacaagcat acagttcact tatgtacggt atgtttcgta aatcattaga ctatgcggtc
      781 accctgcaat ttctgaatga gaatatttcc aaaagaacaa aagcaattcc aaaaggaaaa
      841 tcaatcgttc cttactggac aaaagaagaa tttgaagaag ttttggcaaa aatttataaa
      901 gatgatttct acgaacatat gtgtttttta atgatctggc tatattacat gactggtgtt
      961 cgtgtaagcg aaggattagc acttaattgg aatgacgtgg acttcaaacg taaaaagttg
     1021 agggttcatc acaccttgga aatgacaaat caaaaagatt ttattcggaa accttacaca
     1081 aaaactgaaa gcggcttgcg gatcatttct ctggacgatg atacaattga agtgctaaaa
     1141 aagtggaaag ttattcaaaa aaatcatggt gtagaacaat ttattcttag ctacactgac
     1201 ctcccactct atcgttcaac tgttcaacga attattcagc gttatgccaa actagctaag
     1261 gtacccctaa tacaaggaaa aggattacgt cattctcatg ttagctacct aatcaatgag
     1321 tttaacgcag acattctggt cgtgtcgcaa cggttaggcc attctagccc tgaaatcact
     1381 ttaaagcact acgctcatct atggagccgt aatgatgaag gaattgcaga acaaatgaca
     1441 ggaaacatta aatttacctg tgcaacaaaa tctaaagttg caaactttaa tggaaatcaa
     1501 gcagtaaaaa tctaacccta ccatttccct accaaaatga ggaataggct gaaaaagcac
     1561 tgatataata gcttctttac gaggctactc atattgagtg ggaataaaga gccaacataa
     1621 tatggagggg gaatccctat aaatcaacat tcttcaatag ctaaaatgct tgattttcat
     1681 taaaaataag atggttccct accaaaagag actataatat aagttaattt gtttcattct
     1741 tatcataaaa agaagttctc gaaaaacgaa ctgttttccg agaacttttt tctatccatc
     1801 taagtgctca aatcatattc acgacggaat tctaaggtta ccctttctta aacaaacttt
     1861 atgttatctg cacaactttt tgcaattttt tcattgcttc ttacgaaatc gttgaactct
     1921 ctttagcata atacaaagtg tagatgaagt ctggtgcttt actcgttttg gtaaaaatga
     1981 ctggaaatcg tttctggtta tttttggcaa aggcgaggtg caggttcgat tcctgccttc
     2041 tacgctaacg atgtggctga agggaacctc cgatcttgaa tcgaaacgat ccgcagtcta
     2101 ttgtttcacc aatgaaacaa aatcaacgtt cgtccaagca tttcctgaag ccttgctata
     2161 aaccccaatt tcacaaacac ctgttgtaat tggaatgtct ttgattgtaa tttttgtcca
     2221 ttcacttgtt ttaacaaccg cagtatttag ttccgctcca ccataatttt tgatatataa
     2281 ctgattagta tcaaactctc ccccagacat tacataggca ctcaatgtgt aagttccttt
     2341 gcttatgcca ctaacatttt gataagctga tgcctcgtat gcttgatcct gccaaaaagt
     2401 tagtttgtag tccccaaaaa aagtgcccgc ttccgttttg atcgtaccct tagcggttcc
     2461 ttgttgaaac caggtttgcc aattggaagg actggtcgtt ggtccatcgc tttcgaaact
     2521 tgcattttta actagattta tttgcgcccc tgcttgttta aacgcacgca agcttggcaa
     2581 ggcgtttcca gcaaaatcaa acatcgcttg gttgtcccat tcgtttccca tagtggattg
     2641 aacactgaga taattcatac ctgcttgtgt tgcccaactc acatttccgt tgtaccaaag
     2701 aggttcccaa tagaagaagc ccgccccgcg attattcggt actgcctgaa tccgatcggc
     2761 aagatccagc aaatactctg cttgtcctgt cggtgttggt tcatatcccc ccgcagctgc
     2821 ttcggcttca ccaaaagcat ttggcatagt atcggcattc gctgttgtat gcccataagc
     2881 tgtttcaact acgatcactt ctttaccata ccgttggctg atcgcattca tattggtttg
     2941 aagttcattc attgaaccgt gccaatacgg ataatacgac actccaatta aatcaaatga
     3001 cacacctcga ctcgtgattt catcaaacca ccaagtaaat gctcctgtat cgccaccatc
     3061 agctaagtgg agcattactg gagttgtttt aggttgagtg tctcgaacag cttggatacc
     3121 agacttcaaa aaagcagcca atcgatcaaa ttctcccccg ccttctcccc aagacttgcc
     3181 ataaggccag agcattccgc tattcagttc attgccgatc tgaaccatat caggataaac
     3241 gtccagctgc ttcatttccg acagaacgtt ctttgtatag tcgtacaatg ccgtattcat
     3301 ttcttcaaag gtcagacctt gccatgcttt aggaagattc tgtttcccag gatcaaccca
     3361 aaaatcactg tagtgaaaat ccagcagcac cttaagtccc agattttttg ctcgttttgc
     3421 aaggtcaatc gtcgtttcca gatcatttgt ccctgctcca taactttctc cattactatt
     3481 ataaggatcc tgccacaaac gcagtcgaac atagttgaca ccattatttt ttaaaatctc
     3541 taaggcatct tttgaaatat cgtcttcata atattttgca ccagattttt ccatatctga
     3601 caggatcgaa atatctgctc cgcgaataaa ttcgctggca gatgcttcaa cagtttttga
     3661 aacagccccc aaagccccta acacagctgc aaataaaaca gctttacgca accaaattcc
     3721 cattttcatt atggctcctc ctttattgaa attggttcag aataaactga acagcttttt
     3781 caggatctct cgttaatccg atatattctg ctccttttgt cgcaaccagc ttgattccta
     3841 agcgatcggt gtcttctttg atatcatcat agtaagaatt gacttgcggt gctaagacca
     3901 ccatatcaaa atcctgcagc atttcgtaat gagaaccata agcacccgcc attgcactga
     3961 tcggtgcttt cgattcagat gcaccttttt ttaaagcatt agctaacatc gcacttgtcc
     4021 ctgcgccagc acaaagtacc aacacattga tttctttatt tgcatcgctg ataacttgac
     4081 tcgctgccac aggtacttcc tcacgtacat cggcagaagc accttcctcg gttgcgatca
     4141 cctctgcttt ttgtgcttct ttctctacta agacagcatc gtatgctttg caaaatggta
     4201 gatagatcag gaagtcaacg acgattagaa ccgctgctag aacaaaagct aagataccca
     4261 tgcccgttcc tagaaccaaa ccgatcggtg ctggagttgc ccaaggcaaa acgtacataa
     4321 agctgttcat gcccaataca tcgacaaaaa acttgaacag gcaaacgttt acaacaggtg
     4381 ttaacaaaaa tggaataaaa aaataagggt tcaaaatcat gggtgccgca aatagcaatg
     4441 gttcgttgac agcgaaactt actggaataa aggaagcttt cccaactgcc ttcaactgct
     4501 tagacttagc aaagagtagg aagataaaag gaacaaccaa tgttgcccca gttcctccca
     4561 tagttcctac aaaattccct aaacccaccg tcagtacttt agaagcttgt tcaccggaat
     4621 tgaacaattg cagattggtt tccacattgg catagataat tgccgcaatt gccggctcca
     4681 cgatcgacgg tccatgaaca ccgacaaacc aaaacagcgc catcgctccc cagataatag
     4741 cgattcctaa gtacccgtca gctgctgtaa atagcggctg cagcaccgta atgattgctt
     4801 ctgcaaatga gatttggaag atttgtcgaa tgcctaaatc aattaaaacg gccgcaaata
     4861 ctgaaaaaga gaaaggaaaa atatctcgga aggtttgcga aatcgttcca ggaacttctt
     4921 taggcatttt aatggtaata tctttcagca cacaaaactt atataaatta actgttaaaa
     4981 atgcagcgac aaaggaagcc agtagtccct ttgtccccat atattccgtt gaaaatccac
     5041 cttcaatcgg agagacgctt aacaacagga aacttaccac agaggccagc atcacagagg
     5101 tggtgttgat tactttgttt ttcggtaagc gacgattcat cgaatcagcc aagcagcgag
     5161 cagtggttgc tgaaacgagt aaccctacaa cacccatcga ataattatag actttccata
     5221 gccatgtgga gacagattca ggccattggt aaccaaaaat ctcaggaacc gcagcaatca
     5281 aaatgaaaat gcttgaaaat aaaatagctg gcatcgctgt taaaaatccg tcacgaacgg
     5341 ctcctaaata actattacgt gagatttttt caaaaaatgg tttccctttt tcgatttgtt
     5401 taatgattcc atccatagcg ttcttctcct atccttgatt ctgtctgtac agtacaataa
     5461 agtcttgaat cagatctctc agcaataaag tggtcattaa atgatcctgc ccatggataa
     5521 aaatgaatcc catctccatc tctttcccag atgcttcttc cgctaaaatt tttgtttgcg
     5581 aattatgtgc aagggttaaa ttttcgtcgg cttctttcaa cgccgaatca acatgttcaa
     5641 agtttccttg tcttacttct ttcaataatt tcaataatgt actgcgagcg tcgcctgaat
     5701 atgcaacgat ctcaaaacca agcatttgta attcctcttt attcatcttt tcttcctcca
     5761 ttatcactta ttaaataatc gttttggttt cactgaccaa cttgtaccag taagctgatt
     5821 cttttgggta acgcttctgg gtttcaaaat caacataaaa caagccatac cttttgttat
     5881 acccatttgt ccatgagaaa agatccataa gcgaccacaa aaaatacccc ttcacattta
     5941 ccccggctgt tatcgccttg cttaaggatt ccaaatagac tctcaaatag tcgattctcg
     6001 gctgatccat aataatgcca tcttcaaact gatctttata ccccatcccg ttctcagtga
     6061 tatagatttt gttgtaatgt gggtaatcgc ttttaattcg cagcaacaag tcatatagac
     6121 cttctggata aatcagccag tcccaatcag ttcgaggaat tccttcttta taaatacgtt
     6181 ctccgattcc cttcacttta taaacagagg tccctttttc acccgtaccg ttatgatgga
     6241 ttgcattttc tccatcgtaa gctttgacaa aatggcattg gtagtgattg atccctaagt
     6301 agtcattacg cgtggatgct tttttcaatt caacgaaatc ttcttcagga aaatgatagc
     6361 tggcctgatt tgcttcacat atctcatcaa gagcagtcaa ggtttcagtt gaataatagc
     6421 ctaaataggt agcatctaat aagaaacgga tcgacaatgc atcgtctaaa aaggcagcat
     6481 gcttatcttc cggcgcgtct gttgccgcat atttcgtctc taatgagtga actacaccaa
     6541 tttcacccgg aagttcgttt tctttgaaat agttcactac acgggcatga gcaaccatca
     6601 tattatgaag acaggcgacg atctttgtaa aatcatattt gattcctggt gggaaaacac
     6661 ctaacaaata ttgattggtt gcaactggat agatttcatt aaaggtactc cacaccttga
     6721 cttctttgaa ttcatgaaaa caaaaaatgg cataggaaac aaatgcttct attgtttctc
     6781 gattcaaaaa atctccatgg tcaaacaaac gtttaggggt atcaaaatga tgcaacgtca
     6841 caaacggcgt tacgtttctt ttcttacatt cttcgaacac acgatgataa aaagcgattc
     6901 cttctggatt tggtttccca gctccatcag gaaaaatgcg gctccaagca atggataatc
     6961 ttagaccatt tacaccaaaa cgttcacaca attcaatgtc ctttgcatat tgatgataaa
     7021 aatcacttgc cggatcggga ctgaatcgcc cttgttcctc taaaaagtca tcccaagcaa
     7081 cagcgccttt gccaccttcc ttagtggctc cttcaacctg ataggctgct gtggctccgc
     7141 caaaaataaa atcttctggt aatttaagca tgtacatctc tcctcaaatc tataatcagt
     7201 tttcgctctt tctaactgat tgcattgtaa acgcatccaa aacatttgtc aacaaaaaca
     7261 aacacaaata atcataaaaa ccagaaaaca gcaataaaat ctattgtaac aaacataaaa
     7321 accaccctaa atgtttactt ttgttcgaat tagattgact gttgtccgtt tatatgatag
     7381 attatcctta aattagaggt gaagacaatg ttgaaaaaag aaagattgct tactatcgta
     7441 gaaatggtca ataaaaaagg aatccttacc gttaatgaaa tcatcaatca attagatgtt
     7501 tctgatatga ccgttcgcag agatttagat gaattagaaa aatcgggcaa gctgctgcgt
     7561 gtccatggag gggctcaaag tctttcctac actttagatc aagaactttc tcacactgaa
     7621 aaagccactc tgcagattca agaaaaaaaa gaaattgctg ccacagcagc ctccttgatt
     7681 cacgagggag aaacgatttt cttaggacca ggtacaacaa tcgaactatt ggcgacgcat
     7741 ttactgaaca agaaaattcg gatcattaca aataattatc cagtttttga tattcttgct
     7801 caagcagatg gaccagaaat tctactgatt ggcggtgatt tccggaaaaa taccggcgca
     7861 tttgtcggac cgatcaccaa cgacaattta agaagactaa agtttacaaa agcatttatc
     7921 agttcaaatg gcgttcacaa cgaagcgatc tctacttaca gtaccgaaga aggagaagca
     7981 cagcagatcg ctctaaataa ttcccgtaag aaatatctac tcgttgataa taaaaaattc
     8041 aatcgagatg atttttatgt gttttataat ttgcatgatt tcgatctgct cattacggat
     8101 cgccagatct cgcgggaggt tcaagagcac tatgcacaat atgtcgatct gcaaattgct
     8161 gattcaaccg aaaataccac tataggagcg taactatgaa agggattata tttgatttca
     8221 acggaacaat gtttttggac tctcatctgc atgaagctgc ttggctgcat atgatacatg
     8281 atcatacaaa agatggttta tcagacgagg atattttaaa aaatattcat ggtcggacga
     8341 atacggagat tttgaaacat ttcatttcag agcatttgac cgcctctgag atcgccgtat
     8401 tgtcagaaga aaaagaaagc tattatcgta tgctttgctt gcaaaatgaa aacgagcttc
     8461 agttgacaaa agggcttgaa gcgactcttg accagctgac tgaccaacag attccattaa
     8521 cgattgccac ggctactgtc aaagagaatg ttgcttttta ttttgatatt ttcgatttag
     8581 cccgctggtt tgactgggac aagattgtct atgacgacgg ctctttccct gggaaaccgc
     8641 aaccggatat ttttcttaaa gcggcagaca aactgagctt agctccggct gattgtcttg
     8701 ttatcgaaga tgcttactca ggattacttg ctgccaaacg tgcggggatc ggaacaatta
     8761 ttgcaattga tccattcggt aaaaacaaaa cagtatttga acaggcacag ctaaataaag
     8821 atggaaccat caaagatttt actggttttt ggacagagca cctgtctgcc acatccgtta
     8881 attcttaaac gattcaacta actaccaaag aaaagcaact gaaatgcaca cctcctttta
     8941 taaaaaactg ccagtgaaac gacatgaaag atacactgct atatccttag tgaaatatag
     9001 cagtgtatct ttgacttttt cattctttta accatatgac gcgttcatct ctatctataa
     9061 ttaacattaa aaatagtgtt ttcacattat ttctcaaacc aatcttaaaa atcatcagga
     9121 gtactttttt gatatacttt tattaatgct gggcgtaatg gttaaaagat ttccactagt
     9181 tcctctccac caagctgagt tgaaaaatgc ttatctgtaa acaacataga caaagaggaa
     9241 ataaacgttt ccgtatcttt ctctgtccca aggatattaa ttaattttcc tactaggtaa
     9301 gtatcattaa acttaagatc tgccttcaca tcaaaagttt tgataaaagc ttttatcatg
     9361 cgtacaattt tgtctttatc gccataattg atttcaccac tcatatcaat atagtcacta
     9421 atccctgaag aaataatggc aactgcttct tctttcgcct tatcagataa ttcatttaat
     9481 gactgggatt gattgatgac ttcttgagtt ttatctgtta cgtaatctct cattatccta
     9541 tcggcatact ccgcaaagta atcttgaaaa aattcttcta cagggtaacc aacttcgttt
     9601 tcccaaccag gagaaatttc attatcagta cttattaaat ctattaaaaa ccaacccgtt
     9661 ctctgagtgt tgattctata atatttttaa tttc
//
LOCUS       DACECZ010000093         9676 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.093, whole genome shotgun sequence.
ACCESSION   DACECZ010000093 DACECZ010000000
VERSION     DACECZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.093"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            185..1183
                     /locus_tag="IWO35_002104"
     CDS             185..1183
                     /locus_tag="IWO35_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0317155.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="IWO35_002105"
     CDS             1295..1804
                     /locus_tag="IWO35_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ0317156.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="IWO35_002106"
     CDS             complement(1834..2493)
                     /locus_tag="IWO35_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ0317157.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="IWO35_002107"
     CDS             complement(2495..3259)
                     /locus_tag="IWO35_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ0317158.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="IWO35_002108"
     CDS             complement(3267..3944)
                     /locus_tag="IWO35_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ0317159.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="IWO35_002109"
     CDS             complement(3981..5366)
                     /locus_tag="IWO35_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ0317160.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="IWO35_002110"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWO35_002110"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IWO35_002111"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IWO35_002111"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ0317161.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="IWO35_002112"
     CDS             6305..7162
                     /locus_tag="IWO35_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ0317162.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="IWO35_002113"
     CDS             complement(7243..7578)
                     /locus_tag="IWO35_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317163.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="IWO35_002114"
     CDS             complement(7645..9015)
                     /locus_tag="IWO35_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ0317164.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="IWO35_002115"
     CDS             complement(9093..9629)
                     /locus_tag="IWO35_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0317165.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2838 a         1998 c         1619 g         3221 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaat
//
LOCUS       DACECZ010000094         9656 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.094, whole genome shotgun sequence.
ACCESSION   DACECZ010000094 DACECZ010000000
VERSION     DACECZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.094"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            226..1095
                     /locus_tag="IWO35_002116"
     CDS             226..1095
                     /locus_tag="IWO35_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ0317166.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1304..2329
                     /locus_tag="IWO35_002117"
     CDS             1304..2329
                     /locus_tag="IWO35_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0317167.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2368..3318
                     /gene="manA"
                     /locus_tag="IWO35_002118"
     CDS             2368..3318
                     /gene="manA"
                     /locus_tag="IWO35_002118"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ0317168.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3412..5115
                     /locus_tag="IWO35_002119"
     CDS             3412..5115
                     /locus_tag="IWO35_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0317169.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5117..7150
                     /locus_tag="IWO35_002120"
     CDS             5117..7150
                     /locus_tag="IWO35_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0317170.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7161..7601
                     /locus_tag="IWO35_002121"
     CDS             7161..7601
                     /locus_tag="IWO35_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0317171.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7801..8928)
                     /locus_tag="IWO35_002122"
     CDS             complement(7801..8928)
                     /locus_tag="IWO35_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0317172.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8931..9608)
                     /locus_tag="IWO35_002123"
     CDS             complement(8931..9608)
                     /locus_tag="IWO35_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0317173.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
BASE COUNT         3280 a         1670 c         1970 g         2736 t
ORIGIN      
        1 ccaaacgaaa aataggagga gctatttttg tttggctgtt cttattactt gatgcagaac
       61 agctttttca caacctcttt ttataagagt ttttactcat taatttacaa aaaacaagca
      121 aaatgcgaat tttctcacat tttttttaac aaaatcaaag ttattgcttg aaagaagaag
      181 aatccatcgt taaaataaag cttgacgtag aggggagcaa gaaatatgaa agattttcaa
      241 ttgggggctg ccacctattt aaaaagtgta gccattcggg tgaaagaccg agataaaatg
      301 attgattttt acaagaacgt tgttggcttt gatttgaaaa gagaagaaaa cgaattagcc
      361 attttaggaa cagaagaagc tggaagtgaa atgttgctgc tggaagaaag tcctagagcg
      421 aatgatcaca tgggggtgat caaaaagctg aaccgttttt cattgattat ccctagcaca
      481 gaagaaatgg cagatatcct ttgtcgtatc cgtaaaatga attatccaat caaagaagct
      541 ttagaagatc atggacgtat ggggattttg ctcgtagacc cagaagagaa tgaattggaa
      601 atttaccatg aagagaataa gaacgaccaa agagaaattc cagaaacaat ggatcaagaa
      661 gcattattaa gaaaatctga agaaaaattc agtaaactat caacaggttc ttattttgaa
      721 aaaatccatt taaatgttac taacttaaca aaagaacgcc aatttcttaa tcatgtactt
      781 ggtttgaagg tacataatga agaagagggc atccatgtat tgaatgaagg agatttccat
      841 gttggtttga cagaagctag cggaggcgca attgatttgc ctacagatga agtgattgga
      901 cttgattttc tgaaattcat gatcgatact gaaagcatgg aaggactgat tgctcacttg
      961 cagcaactat cagttgattt cttcttagac aaaaagcaca cagtactcac aatctatgat
     1021 gctataggaa tcgaatggtg gttcgtcagt gagccaaaag aaaagaataa aactgacatt
     1081 cgatgcgacg cttaactttt tctttcttaa cgattgtcgc atgtctcgtc attttatgaa
     1141 cggtcatact atcgcttatt ctagagatag aaagagaaaa tagtataccg ggggaaactg
     1201 cgcggttttt cgcgcagttt ttttgtgtaa aaaaggttga ttcctcatag attaagcttt
     1261 aagcttggac tgtaagaatc tgatagaaaa gcaggagaaa aagatgtatc cacacacgaa
     1321 acagcttatt ttgaaaaaga tggaagaagg attttatcca ggtgtagcag tcgccttcat
     1381 aaaagaaaat caagtagaaa catttacagc cggctttgcc caagtcgttc ctgaaaagca
     1441 gctgatgaca gaagatctgt tgtttgatgc agcctcactg acgaaagtcg ttgcaaccac
     1501 ttcattagtt ttacgtctat tagaagatgg aaagatagaa ctcgatcaac cgcttcataa
     1561 atatcttcct gaatttgaaa atcagacaat cactttgcgt catttactga cacatacttc
     1621 tgatatcaaa acatggatac caaacagaga taagctatct caggaagaat tgatcgccgc
     1681 ttatttgaag ctagaaccag gtgagcaact aggaaaagaa gtaaaatata cagatgccgg
     1741 tacgattctt cttgggttga tgctagagga aatttaccaa gaaactgcag tggaaatctt
     1801 tcaagaacaa gtattggaac cattaggaat gatgaacagc ttatttcttc cttttccttc
     1861 caaaaaaatc gttcccacag aacaactgcc ttcgggtgag gttttgcgtg gacaaactca
     1921 tgatccgaaa gcaagagttt tggctgaaca tgcaggtaac gcgggtctct ttattgattt
     1981 gaaagacagc attacttttg tccaaatgta tttgaacaag ggaaaaatcc gtaatggtcg
     2041 ttttttagaa gaagagacga ttgattcttt actgcaagat caaacaccga aaaaaaatgg
     2101 ctatcgttct ttaggatggg acttaaaaaa ggatgtagaa gacggtcatg cgattctttt
     2161 tcatactggt tatacaggta cgtttttctt gattgatata aaaacacaag aagcgttgat
     2221 atttttgtca aatcgcgtac atcctgtcga tcatcgtgcg gagtatctca tatgtcgaga
     2281 tgaaattgtt gaaaactttt tgaaagagaa agcggtatta ccgaaataat gctataatag
     2341 ctatgaaaaa tgagaggagt ttgaattatg caggaaccta tgtttttaaa accagttttt
     2401 caagaaaaaa tttggggagg aagtcgttta cgttcagtct ttggttttga tatccctaac
     2461 gataaaatcg gagaagattg ggcgatcagt gcgcatcctc atggtgtcag tgtagtagaa
     2521 aatggcgaat tcaaagggaa aagattagat gaactatggt cagaacacaa ggaattgttt
     2581 ggccatcctt cagaaccagt gtttccatta ttgatcaaga tcttagacgc agaagatgat
     2641 ctatctgtac aagtacatcc agatgatgcc tatgggatga aacatgaagg tgaattagga
     2701 aaaactgaat gctggtatat catcgatgct gaacctggtg cagaaatcat ctatggtcat
     2761 catgcaaaga caagagaaga actagctgag atgatcaaag atgggcgctg ggatgacctt
     2821 ttgaagaaag taccagttaa aaaaggtgat ttcttttatg taccaagcgg aacgatccat
     2881 gctattggta aaggaatcat gattttagaa acacagcaaa gttctgacac gacttaccgc
     2941 gtgtatgact acgacagaaa agatgcgcaa ggacaaacaa gagagctgca tatccaacaa
     3001 tcgatcgatg taacaactgt ccctgcgaaa acaccagaat tacaaatcaa agaagtccga
     3061 aaagggaatt catcgatcgt cacttattta gaaactgagt ttttcaatgt atatgagtgg
     3121 gatattaaag gaatcacatc tttcaaaaaa caagcccctt atacattgat gacggtaatc
     3181 gaaggagcag gagaccttgt cgttgacgga aaaacatatc cattagaaaa aggaacaagc
     3241 tgtattattc caaatggcgt ttctgagtgg gcggtccaag gtgaactagc aatcattgcg
     3301 tctgaaccag gcaaatagtt cccagatagc tgaaagcagc agacggtttt tcgtttgctg
     3361 ctttttctac ataaaatccg tccatcaata aatagaagtc tggtgaaaaa gatggaatac
     3421 aaatggaaag caaacaacag cagtcgcgtg tctatgaaac aactgagaaa agctggacat
     3481 cacttaaatg aaatgatcat gccaaatcta agtctgttta tcgcttgggg aatcttgtca
     3541 ttagcaacaa aaggtgtgtc tggcgatttg cacaccacat tagaagaggt atgccattgg
     3601 atgatccagc tgcttttacc catcttgatc agttattcag gcgggaaatc ggtaggggga
     3661 caaaaagggg cagtcgccgg tgcaattgct tctttagggt tgatcgctcg ttccaatgct
     3721 ccacaaatcg taggagcgat gattatcggg ccgcttgccg gcatcgtata tgaacaattt
     3781 gtttctagat tccatgtgaa attcaaggct ggatatgaaa tgcttgtcag caacctacta
     3841 gtaggactga ctggtagcgt tatttgtata attggaatcg tttttattga accagtttta
     3901 aatagtgttc acttgttgct cgcctccgtt gtgtcttggc tagtcacaat gaatctgtta
     3961 ccacttgtca gtctgatctt agaacctcta aaagtactat ttttcaataa tgcaatcaat
     4021 catggtgtat tgacaccttt aggaatcgaa ttctcacaaa cagtcgggta ctcttcctta
     4081 tttttgatgg aggcaaatcc tggacctggt ttagggatct tactttctat cctttgtttt
     4141 gcagaaaaac aggaaaaagt aaatgcgagt ggagcattga tgatccatgc gattggcggg
     4201 attcacgaaa tctattttcc atttgtcttg ctgcgaccgt atctattttt agctgtaatg
     4261 gctggtgggg caagcggcac gttgatattt caatggttag atacgggatt agctgcacct
     4321 gtatcgcctg ggtcactact cgtgattttt gccaatgcgc cttatccaca actagctggg
     4381 gtaagtatag ggatagcaat cagtacattt gtgaccttca tttgtgcagc gatactattg
     4441 aaattaccaa ataaagaaaa agagaacaaa acggtcaaag aaaggggaga aatcttggaa
     4501 ccatctatca ttcattccgt catttttgct tgcgactcag gtatgggatc aagtgctatg
     4561 ggagccgccc tgctgaaaaa gaaattagca gcagaaaaaa tcgatctttc tgtcggatac
     4621 agctctatct atgaattgaa taaccaagta aatcagctgg ttatcgtgca acatgaacta
     4681 gcgggtcttg caagagaaaa agtacctcaa agtcaaatct atgtactgga tcactttttg
     4741 gaaacagata tcttgctgtc acgaataaaa gaagcgatcc aatttccaaa gcgggaagaa
     4801 aaaaaagaga tagcagatat cacagaacaa aaaacgtcgc ctgctaatat tgtatttttg
     4861 tataaaaaca acagacgcgg ctcacaaact atgggcatga cgattttaag tcaattagca
     4921 aaaaaagcga aaaaacagtt tgttatctca aaagaatcaa tagaaaaaat caaaatccaa
     4981 gaagaaacga tttatatcgc acaaagagaa ctagctgtaa ctgaacagct agaagaaaag
     5041 atccctcggc tagttctagt tgaacattgg gtagctacga atgaatacga aaaactcgtg
     5101 aagggagaaa tataagatgt atttatcgcc cagagaaaaa caattattag cagaatttct
     5161 cagtagcccg tcacctgtat ctatccaaaa aatgatgaat ttattgaagg tcagtaaacg
     5221 gactatttac cgcgaattag ataatcttga agtctcattg aaatcggtaa atgcttcttt
     5281 gaaaaaagca gctagaggct catttgtcat tgaggcatca gaagaagtat tagagcaatt
     5341 aaaagaaaat acagatggga aaagtaatga cttatccact gctaaacgac aacacgcgat
     5401 tttagccgag ttgttactga cgacagagcc gttgagtctt gctcatttca tggaatcata
     5461 tcgcatcagt aataccactt tttatgcaga tatcaaacag ttggaagaaa gtattcgcca
     5521 actgcctttg gaaatcattc ggaaccaggg atacgagatc gaaggaccgg aaaaataccg
     5581 ccggcttctg acagcgaatg tattagaatt agaaatcaat gaatatgaac tttttcatag
     5641 tatttctttc gatgcatcac tgaattattt ttttcgattc gttgatccgc aacatctatc
     5701 tcttgcacgc aaagtaatcg gggaagaatt gatccaaaaa aagaccaatc taagtgatag
     5761 aaagcttgaa catttggttt tgatgctgac attgacgatg gagcgtgtga cgaaaaatca
     5821 tttactgaca aatgagacat ataatagtct ggcaaacaaa gaattactac agacagctaa
     5881 acgattgttt tcacggatcg cttctgagac aaaacaactt tatccagtaa atgagatcgt
     5941 cttttttgca ggattattaa atgacttcac gaactcattt gatcagaatt tctttgaaga
     6001 gacgttcgat acacagcttg cttatttagt aaaacaactg attcaagaag taagtgaaga
     6061 gacgaatgtc catttttatg aagatgaaac tttgtacaaa atgctgttga ctcatttatc
     6121 tggtgtgttt tcaagagccg ttttacaaga agagacactg accaatccga ttttagaaaa
     6181 aatcatggtt caatacgagg aagttgcgaa tgcaatccgt aaagcagtag agcggatttt
     6241 tcaagaaaaa aaattatccg aagaagagat cgcttatatg gtccttcatt ttgcgaattc
     6301 attggaacga agtccgaaag aaagcaatat cagtattgca ggattttcgc caagtggatt
     6361 agcttctact agcatgttgg aactacgatt gaaaaggtat tttcccttca tccaacaaat
     6421 tcatttctat cggattgctg atttaaaaaa aatcgattta caagaagaat atgatgttgt
     6481 tatttctaca tctattttac caggatacgg tggaaaatat attttagtct ccccgctact
     6541 gctagaagat gaagtcaagc agctaaaaga agctttttcg catattgaga agaaaaaaag
     6601 gcaaaaaatt acgtataaca gcaataacga gtggatgata gaagatactt atgaggaaac
     6661 aatgcatttc atggatcaaa tcaatcaatt actcggtcgt ttttttatcc aagaactgga
     6721 taatcctgag acgatttccg aaacgattcg cagcttattt ccttattttc ctgaaggact
     6781 agtaacggat gaagaggtgg ttcatcgccg gctgcttaag cgatatgaac aagcacctat
     6841 cggtattcct catacaaata tggggttatt ccatacatcc agctcattga tttctaaacc
     6901 gatcttttgt attttcaatt tgaaacatcc gttgaccatt gaagggatgg atagacagcc
     6961 agtcgatttg tcccgtatgt tagttatgct ggcgccttcg ccaatccaag aaaaagacgg
     7021 gattctttta ggaaagatca gtggagcaat catcatgaat gacttgaaca ccgagatctt
     7081 ccgctcagga aataaagaaa tcgtttatca gttgttagcg aaaatcctga ttgaagaaat
     7141 aaagaaatga aggtgaaaag atgatacagc taccagcaga aaaaatattg ctcgatcaat
     7201 cctatcaaaa ttggaaagaa gcgttgaccg agatcggcca gtggatgacc caacaagggg
     7261 gaatcaaacc ggcatatatc caatcaatga tcgaaagaca agaaaaagcg agtgtctata
     7321 tcgggaattt cgtagccttg ccacatggga ctgacaaaga tgaagaagta gtagaagaag
     7381 gaatctattt agtccaagtg cctgatggtg tggactttgg aacaaaagag gaaccaaaaa
     7441 tcgcgacact cttatttgta gtggctttaa aaaaagaaca tcagttagcg gtgctccaag
     7501 atttagcctt tttctgttca gacatcgacc aagtaatggc gctcagtgat gcggaaacca
     7561 ttgaagaagt ccaaatgatt tttgaacatg gacatatata accaatgttc acagtaaaaa
     7621 aagctgcctt gcatcaagta ataacttcaa ccaagataaa aataagagga gctatttttg
     7681 ttgggaggtt cttattactg cggatgcaag cttttgaaaa ctatttattc tagaaaaccg
     7741 tttatttgaa ataggggacg gtgacaaggt ttttgtcacc gcccctatta tttttgtgtt
     7801 ttaatagctc ttttttatat agatagtgcc catacctatg aataataaaa cgatactcca
     7861 tataccaagt gtcaagatac cattcatgaa agtaacctgt tctgaagcta gtggcgagaa
     7921 aatatctcct cctatgatca cattttgaaa atcaacataa gaagttggca agtaatgact
     7981 gattgattct atccatccaa tcgaagaaaa caagcgatac tctgctaaga aaagacaaag
     8041 aagtgtacct aaaagattga ctagagaatg ttcaaataaa gcagatagaa gaaaactgat
     8101 tgtacttaaa aagagcaccc acaacaacag gaacagaaag ctctggaaaa gatactcata
     8161 catcggcagg attcgtatct cctgaccaat gatcgttcct acaggataac ggaagtctcc
     8221 caaaccattt ttgattatta ctagtataga gacgatcaaa aatggcatca cgagacttag
     8281 catagctgca ccataataaa tggtaaaacg gatgctatgt ttctttacca tactatccgg
     8341 taatagattg actagagaga ttacctgctt tcttttttca taagagatga tggaagaaag
     8401 aaacaaggcg gttatacata aaatcaaaaa actagaaatc attcctgaaa aaatattttc
     8461 gtagtaattt gctaaagaga gatgcgctgg caagtcaaaa atagaatatc tttggatctg
     8521 atgttctttc atataacgga gcaactcgac acgtttttgt tgttcaatga ttggaattcc
     8581 ttgtctttga ccagaaatca gtcgatccag tatatctttt tcataatgat aagttgcttc
     8641 gaccatttgc tggacattcc ctgtgttttt tgcatgtaag attgcttcga tcagtttgtt
     8701 gcttttctct aaactttcca caaattcttt ctcattctca tcttctttca acgtttcaat
     8761 caaacgttgc atctcttcat agttatgatt cagttcatcg aattctgtat aagcaagttt
     8821 atgatcaaaa aaaagtggga atgcaaagag cacgaccaac aatagaggaa ataaaaagtt
     8881 tactttgttt tttagtaaga gattgcgttc aaatgtaagt aaagacatag ttacacctca
     8941 tataatttac gataaatagc taaaatctct tccatatttg aggattctgt gacgatttct
     9001 ccatttttta gaaaacatgc tttagaacaa atcgattgga tattcgcgat agcgtgggaa
     9061 gaaagaagaa tcgtcttccc ctctttctgc cattgtctga tgaggctatt caagatatca
     9121 atacttcctg ggtctaatcc gttcaacggt tcatcgaata acatgacaga tgaatcactg
     9181 atcaagtaag cagcaagcaa gacatgttgt ttcattccta gagacatttt tccaatcggc
     9241 aaagtaaggt actcagtcat cttcaatcgt tggaaaacag tttggcattc tactttgctc
     9301 tgccaatagt atttgatcaa agtcaaataa tcaacagccg atagatagtc ggataactga
     9361 ttcgttgatt ctagaaaaaa gaaagaccgg cgataatctg tataagcagt aggagagatg
     9421 ttgtgaaggg acaattgatc ataagttgtt ggcagcagtt gggcaatgcc attcagcaaa
     9481 gttgttttgc caaatccgtt cggggcaatc aaagcagcta cttctcctga ttctactaaa
     9541 agattcaatt gtcgaatcac acgtttttta ccacgtttca tgttcatgtt tttgatcagt
     9601 aatgtcatag ataggcctcc aatataaaat ttaataacat aatagaatgg aaaaca
//
LOCUS       DACECZ010000095         9407 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.095, whole genome shotgun sequence.
ACCESSION   DACECZ010000095 DACECZ010000000
VERSION     DACECZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.095"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(155..1204)
                     /gene="recA"
                     /locus_tag="IWO35_002124"
     CDS             complement(155..1204)
                     /gene="recA"
                     /locus_tag="IWO35_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ0317174.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1313..2554)
                     /locus_tag="IWO35_002125"
     CDS             complement(1313..2554)
                     /locus_tag="IWO35_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ0317175.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2794..3372)
                     /gene="pgsA"
                     /locus_tag="IWO35_002126"
     CDS             complement(2794..3372)
                     /gene="pgsA"
                     /locus_tag="IWO35_002126"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ0317176.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3581..4465)
                     /locus_tag="IWO35_002127"
     CDS             complement(3581..4465)
                     /locus_tag="IWO35_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0317177.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(5243..6529)
                     /locus_tag="IWO35_002128"
     CDS             complement(5243..6529)
                     /locus_tag="IWO35_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0317178.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6522..7790)
                     /locus_tag="IWO35_002129"
     CDS             complement(6522..7790)
                     /locus_tag="IWO35_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0317179.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            8042..8503
                     /gene="mscL"
                     /locus_tag="IWO35_002130"
     CDS             8042..8503
                     /gene="mscL"
                     /locus_tag="IWO35_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ0317180.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8562..9008)
                     /locus_tag="IWO35_002131"
     CDS             complement(8562..9008)
                     /locus_tag="IWO35_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0317181.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(9101..9346)
                     /locus_tag="IWO35_002132"
     CDS             complement(9101..9346)
                     /locus_tag="IWO35_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317182.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT         2601 a         2034 c         1663 g         3109 t
ORIGIN      
        1 ctattaaatt attctttgta gatgaacaga tatttttatt ttatgtaccc actcgtataa
       61 ttaaataact cacgatttct ttattcaata aaaaaacggc aggtcaagga gagtacctac
      121 tcttcagacc agccgttttt gtttatcata atttttattc ttctactaaa ggcaattctt
      181 cttgtccttt ttcatcttct gggacagcga cttcttcacc aataccataa gctgctctaa
      241 ctagagcaga aacttctgcc atcatttcag gatgattagc catatagatt ttagcgtttt
      301 cccgtccttg accaatccga tcttctttat aagaatacca agcaccgctt ttatcaacaa
      361 tgtctttttc tactgccatg tctagaagtt ctccttcttg tgagatccct aaaccataca
      421 tgacatctac ttcagcgatt ttgaaaggag gtgctacttt gttttttacg actttgatct
      481 ttgtacggtt accaacaata tctgtacctt gtttcaattg ttctgcacga cgtacttcca
      541 aacggatcgt tgcgtagaat ttcaatgcgc gtcctccagg agtgatttct ggattcccaa
      601 acatcacgcc tactttttca cggatttggt tgatgaaaat agcgattgtt tttgttttgt
      661 tgatcgaacc agagagttta cgcaatgctt gagacatcaa acgtgcttgt aacccgacat
      721 gtgagtcacc catttcgccg tcgatttctg ctcgtggaac taaagcagca actgaatcga
      781 ctactacgat atctacagcc ccacttgata ctaaagcatc agcgatctct agaccttgtt
      841 ctcctgtgtc aggctgtgaa agaagtagtt catcgatatt cacacctaat ttttgtgcat
      901 attgcggatc taacgcatgc tcagcatcaa tgaaagcggc cgttccgcca tttttttgta
      961 cttctgcaat agcgtgtagt gcaactgttg ttttacctga actttcaggt ccatatactt
     1021 cgatgatacg tccacggggg taaccgccga cacctaaagc gacatctaac gcaagagagc
     1081 cacttggaat tgtagagatt tgttggtcga ttttttcgcc caatttcata atagagcctt
     1141 taccatagct cttttcaatt tttttcagtg cagcatctaa ggctgcttta cgatcatctg
     1201 ccaatacgat aatcctcctt atatttacaa atcagagcaa catttcatta tccttatcat
     1261 atctttttta gtgaaaaaaa gcaagaaaaa accgaacaaa tattcgcatt tatcattttc
     1321 ttaaaagctg ttttctcaaa agatcaaatc cttgcataac cgcattatat ctgatagcag
     1381 cacgatctct ggcaaagtga tatttttccg aaaccacgcc gtctttagat gaaatagcga
     1441 tccatactgt tcctgcagat tgaccttcta acgtgtccgg tccagccact ccggtgaaag
     1501 ctacactgta atctgttctt gctatctcac gtgcttgaat ggccatctgc tccacacatt
     1561 ctttgctgac tgttccatat tctgctaata gctccggatc aatctccaga aaaccagctt
     1621 ttgtttggag cgagtaggta acgaatccgc ctggaaatac ttcagataca ccagcaatgt
     1681 cgcctaatgc ggcttggaag gcgccggctg tcaaactttc cgctgctgtt acagtctgct
     1741 tgttttcttt cagtagttcc actacgactt ttgctagcga attatcatcg ccgtatccat
     1801 aaaaatattc ccctactcgt tcttggattt tttcttctaa tgctaaaaga gcttgattcc
     1861 ctgcctgaac atcatttgtt ttcactgtca gtcgtaatgt cacttcattt ggtttggcat
     1921 atggtgcgat cgttggattg atctgtgtct caatcagatc ttttagctca gtgaccagtc
     1981 gcgattcacc aatcccataa aagcgcaaaa ctttagaaat tagtttttct tcagaaggaa
     2041 aacgttcctc tagtaaagga cgtacttgtt ccacaaacat tggtttcaac tcattaggcg
     2101 gaccgggaag aaggatatac gcatgtgtat ctgtttgata aaaagtccct aaagcaagcc
     2161 ctgtacgatt tggtagagga accccgcctt cgataatctg ggactgttgc agattgtttt
     2221 tggtcatttt tcgatgggtt gtttcaaaat atgccaataa ttttttgtat ccttccgtat
     2281 tttgtatgag agacttaccg aggtgagccg cagttgcttc ttttgtcagg tcatcttctg
     2341 tcggtcctaa tccgccacat aagagtatga gttcacttct tgtttcagct agagcaatca
     2401 actcttctaa acgctgctga ttatctccta caactgtttg ataataaact tcgataccta
     2461 gatcagctag ctgttctgac aagaaagtgg cattcgtatt cacgacttgt cctagtaaaa
     2521 gttctgttcc gacagctatt atttctgctt tcattacttc ccgctccctt atatagtata
     2581 cgctgtattt ttatattttt ttatttaaaa agagggcaag gagctgtgac aaaaatcttg
     2641 tcacagctcc ttgttccaaa taaacagtgt tctcctgcca aaacctcggt cataagtcac
     2701 aatatacata aaacatgaaa agctgttttt tgaaattatc ccttgtgact cagagttaga
     2761 cggcaggctc acaacctctc cactaaacga tacttacatt gatcctttga acacaccgct
     2821 gtttttgatg aagtaatcca cacctgaata gatagtaaag atcagacaag cataaagcat
     2881 gatttgatct actggaatac cgatcaggct gaatgggata ttattgataa acagcaagat
     2941 aatagcaacc atttgtgtag ccgtcttgac ttttcccggc caagctgcag ccatgacttc
     3001 tccatgttcg acaagtagta aacgcaagcc tgtgacggct aattcacgac agacaatgat
     3061 cgctactacc caaccagggg cctttccttg gtcaaccaac atgataaaag cagtcataac
     3121 tagcatttta tctgccaacg ggtccgcaaa cttgccaaaa ttagtgacaa ggcctcgcgc
     3181 acgggcaatt tttccatcta accagtctgt gatactagct aaagcaaaaa taactgcacc
     3241 tactagctgt gtgacagcca gtgtggtgtc tcctacgtta aattgtcccc aatctaaagg
     3301 taaacctaca accaaaatga aaatcgggat catacaaatt ctaagtacag ttaatttatt
     3361 tggtaaattc acactatcga ctcctttctt ccctattcta cgtgactatc agcgaagttt
     3421 ccaccgatat tatttacttt tccaaatgaa tttattcaaa ggagtatttt tattgaaatt
     3481 gatctttttg aattaacgaa gaaggaccgc gacacagatt tgtcacgatc cttctttttt
     3541 atacctacaa gtaaacagat gcgtaaacta atttatacga tcattctgcg tattgaagag
     3601 taaggttcac attttttgga ctctgattgt tttcccctgg attaactggt acggattctc
     3661 cattcgcttt gatcgttgca ttgcttgctg caccaatcgt gatggtcgca tttgttgctg
     3721 tttccggtaa cacggtactc tgtgtttcat ttgctgccag tgtcccttga taaacatagg
     3781 cattgtctac tagcacacct aaccatacac gattgacagc agtaaattca agggttacag
     3841 gcttggtagc gtctgtgata tttactgcga tggcactgcc agaatcttct cctgtagcta
     3901 ttgccatttt cttttcttca cttgattttg ttgttgattc tactgtagaa gactctttgg
     3961 tctgcgtgct ttgctctgag gaagaggact cctcgactga tccttctact ttaagcgaag
     4021 aagctgtgtc tccgattatt ggatcagctt gacgatcttg ccaagtcata tagcccacga
     4081 caacgacaat cgccaaagca accaatccta ataaaatgac aggaagactt gtccaaaagc
     4141 gacttggatg cgattcttcc tgatgcattg cttttctaga agtacttact gtttcaggct
     4201 gcggacgttc agggaccgat cctgtcaatt tgcttttccc atcaaaaaca tcgactaaat
     4261 ggtctccatc ttctcctaca gcttcagcgt attgccgaat aaatgcccgc acgtaaaatt
     4321 taccaggcaa ttggtcaaaa tcatcattgt cgattgcttc caaataacgt ttttgaattt
     4381 ttgtaatctg ctgtaattca tctaaagata tttttttatt caggcgtgct tgccgtaatt
     4441 tttctccgat ggtttcaaaa gccactcgtt catcttctcc agtctgtttg attctccgaa
     4501 gcatctgctg ttgcaacgaa agacgttatg tttgggtgct gtgacaaaag tcatgtcaca
     4561 gctccttttt tccgaataaa cattgttctc ctgccaactc ctcggtcaca agtcacaata
     4621 tccaataaat ataagaagct gttttttgaa cttgttcctt gtgactcgga gttaaacggc
     4681 aggctcacga cctctttctt aaacggtgtt caatcagctg ttcctcggta ttagctcaaa
     4741 aaccggcaaa atataaggag atatttttcc gtatgtttct ctaatactca ggtcaaaacg
     4801 ctgatttcac aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg gtaataagtc
     4861 caaccaagtt aaaaacatta agaagctgtt tttacttggc tgttcttatt acttgatgca
     4921 gggcagcttt ttcacaacct cttggtacac ggtgttccag aagtagcttc ttcggcaata
     4981 agccgaaata tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
     5041 gctgaccact tctgtcacaa cctctttgat tacttcgttt gattttattt taatataagt
     5101 tgattaaaag agagcatgat ttgttttatt ttctcactaa tttttccaac acaactaaga
     5161 ataccataaa agctgatttt ggaggaggtt cctactacaa gtctaaaacg aaatttaaga
     5221 cttttcctca taaaatactg gtttattttg gtctcatgta aaactcgctg aaggcctctt
     5281 cacttataaa tgcttcccca gctgccagta cgtctttcat ttggatcgaa tcgatgattt
     5341 caggaagatc aaataaagtt cgatcgccaa ataaactttg cgtaaattga ttggcaatat
     5401 attcgatcga atttaatgat tggaaatatt gtccaagcat tttcttcttc agcaaatcga
     5461 gatttgtttc tgacacttcc ggatcatcag cgaatccaag aatgatttct ttgacttttt
     5521 ctgctgcttt ctctggttcg tccgtatctc cgctgaagtc cgcaaagtga aattcacgat
     5581 ccaagctgaa ttcaaaacca aagctgtcat cgataattcc ttgattgtac atcgctagat
     5641 agtttcgaga agtattgcct aaaatcattt ggaacaacag attgattgct gttttatagc
     5701 gtaacagttc tgttccttct tgcggcaatg tgtctaatcc tttaatacca agtacaaatt
     5761 tatctcgtgt aatggctgct tccaatgcac tttgtttgat gatttctttt gtgttttcag
     5821 ggaaataacg tacaatttct tgctttggag ggaaattttt tgcttcttgg ttttcccgaa
     5881 taagtttcat caatttctca ggctccatct taccaactac aaataaaacc atattgcttg
     5941 gctgataaaa cgtccgatag cacgtataca agtcttgagc tgtgattttg tcgatacttt
     6001 caaccgtgcc cgcaatatcg atatgcaatg ggtgtgtagg atacaagtta tttagtatgc
     6061 caaaaaacat ccgccagtta gggtcgtctt catacatttg gatttcctga ccgatgattc
     6121 ctttttcctt gtttacagtt tcttctgtga agtaaggtgc ttgtacaaaa tcgatcagag
     6181 tcgtcagatt cttttctacc tgatccgtcg tagaaaacag atagctggtt ttcgtaaaac
     6241 ttgtgaaagc attggcggaa gctccttgtt tcccaaataa ttgaaagaca tcaccatctt
     6301 ctttttcaaa caacttatgt tctaaaaaat gggcaattcc atctggaact ttcacctttt
     6361 ctttttcacc atagggaata aattcgttat cgatcgaacc ataattcgta ctgaacaaac
     6421 catatgtctt gtgatagtca tttttaggca acaaatacac ggtcaaaccg tttggcagta
     6481 cttcatgata aagtgtttca ttgatttgct catattctgt tttattcatt tgcatttcct
     6541 cctgctaaaa agaagattgc ctgcaattcg atttgctcag ctacttgttg tatctctttg
     6601 atcgttacgc tctccatacg tttcaaccat tcttcatcag ataattttgt ttccggcagc
     6661 cagctgtcca aaaaagaagc ttcgattgcc gcttgcggag agtctagaga aagcagatat
     6721 tggtttctca gcattgcttt tgtctgagac agttccaact ctgttatttc accatttcgc
     6781 aaactttcta attgctcgtg aatcagatga agtacttgat ttcggttctt ttcatcgata
     6841 ccggtttgga cactcatgaa tcctcggaac gtatccacgc tactagaggc ataataagct
     6901 agactttctt tctctctgac attcatgaaa agtttagagt gaggaaaacc gccaaataaa
     6961 ccgttaaaga ccattagcgc aaaacgttct ggttcatcgt aatacacatt tgtctgataa
     7021 gctaggttca gctttgcttg aacgattggc tcacgtactt gtcgttcctt cacgatattc
     7081 acttgaggtt gggtataaaa cagatctgga taaactgttt cccgcgactc aaatggtaat
     7141 tgcccaactg cttcagctgc tttgtccgga tcaacatcgc cgacaacaaa aatgtcgatt
     7201 tgatcatttg ccatcatttc ttgatagtag gacgctagcg aactagcagt gatcttgtcc
     7261 aaatcttcga ctacgcccaa ccctgggatc ttttgttccg ggctgttttc aaaataaagt
     7321 tcttgcaagg cataggaagc aaaagtttgt ttatcttcct tcaaactttc caaatataga
     7381 cgcagattgt tcttttccaa gtcaaacgtc aactgatcaa actgatgatt tttaatattt
     7441 ggataaaata aaacctcttt taaaaaatct accgcttgag aaaaaaggtt tggatcattg
     7501 acatattcac cattcacaaa actgatccct gcgtttagcc aatgtaaatt tccttttttt
     7561 ctaactgata atccaaagct tgcgccataa agctctgcaa gttgcgcact taactttgtc
     7621 tgatccgaat aattcaaact atttgtttcc aacatgctgc tgagaagtga acgtttcgtg
     7681 atgacttctt gttgcagacg cgcagtaaaa cggataaaaa tccggatcgt tttgtattgt
     7741 tttgttggta agacatgaag attcacgcct tctgccaatt gatacaccat atttatatgc
     7801 tccttttcac agttgattgc ccgcttactc ttttaatggg tcaacctgcc ttttttactt
     7861 attcctctgc ttgaaattta taagttcagg caattcgccg ttttttctaa atgatgttac
     7921 actttatagt atagcataga tgttttttat caaaagaaac agacggctaa aagcgtatat
     7981 tgttttttta ttttttattt ttcgatatac taaagacaaa taaaagaagg gagggtcatt
     8041 catgatcaaa gaattcaaag agttcatcat gcgtggagat gtcttggatc ttgctgtcgg
     8101 cgttgttatt ggtagtgcgt ttacaagcat cgtaaaccaa gtggtgcaag gcttgatcac
     8161 accactaata ggttggtttg tcgcaatgat cacaggtact tctgatttag aaggagcact
     8221 ttcaattctt gactggtcgc cgacaaaagg cgtcacattt gcgtttggta acgttattag
     8281 tgctatcatt acatttctga tcactggttt tgtcttattc ttagttgtca aagcggcaaa
     8341 ccgtgcgaag aatttacgct ccaaagaaga gcaagtggaa gtggaagaaa ctcctactgc
     8401 agaagattat cttagtgaca tacgcgatct tcttgccaaa caagtggcag aagacaatac
     8461 acatccagta gagcaaacaa atctttccga tacttctaaa taataaagaa tctaaaaaaa
     8521 agaaagccga ttttcagtgt cggctttctt tttttagatt cttaacttgc tacttgttcc
     8581 tttttcatgt cgatatgtgg aatatcatct tccaaataca catctgagat tgccttgaat
     8641 ccaaacgact cataaaaagc ttgtaggtat tcttgtgcgc cgatcacgat tggtttttct
     8701 ggaaaatttt tctggatcat gttaatcgtt tcttgaacaa tctttcttcc taaaccatct
     8761 ttacggttgt cctgatgaac aaggacacgt ccaaaatgga tactctccgc ttcttcatat
     8821 actcgcgtgt aggcaaggat cttcccatca gtatcttgaa aaaacgtatg ccaagcttct
     8881 ccatcaatgt catcaacttc ttgataagga cactgttgtt ccacaacaaa aactgcgatt
     8941 cgttctttga cgatcgcgta aaactctgct gttgacaatt cttcaaattt ctttacctga
     9001 tattccacta agcaaacgcc ccctgctttt ttattcttag cataccgatt cgcacacaaa
     9061 aaagcaaacg aaccttacga atctcaggct cgtttgcttt ttaaactgct tcagttaaaa
     9121 attcgatctc aactgtccga gtaagtacat ctgaatagct gtaagatact cgttcaaaag
     9181 aattttcttc aggatccaaa tcaacaacga atactgaagg ataggtttct gttaaaacgc
     9241 cgcggcgttc tgtttgacgc tttcttcctg tctgagcaac caacatgatt gggctgccga
     9301 tacgaccttc taaatctttc ttgattgaag ctaaagttgt tggcatacag ttcacctcta
     9361 ctatcaacta taccacatat ttttaaaatt ttcaagttta ccataaa
//
LOCUS       DACECZ010000096         9390 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.096, whole genome shotgun sequence.
ACCESSION   DACECZ010000096 DACECZ010000000
VERSION     DACECZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.096"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(121..789)
                     /locus_tag="IWO35_002133"
     CDS             complement(121..789)
                     /locus_tag="IWO35_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0317183.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            923..1210
                     /locus_tag="IWO35_002134"
     CDS             923..1210
                     /locus_tag="IWO35_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317184.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1626..3164
                     /locus_tag="IWO35_002135"
     CDS             1626..3164
                     /locus_tag="IWO35_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ0317185.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3161..4126
                     /locus_tag="IWO35_002136"
     CDS             3161..4126
                     /locus_tag="IWO35_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0317186.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4113..5435
                     /locus_tag="IWO35_002137"
     CDS             4113..5435
                     /locus_tag="IWO35_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0317187.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5498..5602
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5676..6419
                     /locus_tag="IWO35_002138"
     CDS             5676..6419
                     /locus_tag="IWO35_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0317188.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6434..7426
                     /locus_tag="IWO35_002139"
     CDS             6434..7426
                     /locus_tag="IWO35_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0317189.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7438..8175
                     /locus_tag="IWO35_002140"
     CDS             7438..8175
                     /locus_tag="IWO35_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0317190.1"
                     /translation="MSIQISHLNVVIENLKVLEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8165..8887
                     /locus_tag="IWO35_002141"
     CDS             8165..8887
                     /locus_tag="IWO35_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0317191.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8971..>9390)
                     /locus_tag="IWO35_002142"
     CDS             complement(8971..>9390)
                     /locus_tag="IWO35_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ0317192.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3077 a         1611 c         1977 g         2725 t
ORIGIN      
        1 tttcgcagat cgtttgcaaa aatagtttat ttttttataa agaagatttt actgaataat
       61 atcattttag tttacataat gatatttctg taaattaaat aaagtagtga aaaataagaa
      121 tcagtgctct aactgttcaa gaggatcaag tggttcaaat gtttctgtag tctgttttgc
      181 ttccaattgg ggcaaaacag atccaatttc cagatacttc actttattca atgtataaga
      241 tagaatagaa cagctaagta atgtatacag aaaatgtgtg agatccaaat aaagcaagga
      301 gacaactaaa ataccaaaag taatcaagaa aagacttttg ccaatcttaa tatgaaaata
      361 tttttcaatg gcttgataaa tgatatctgt tccgcctgtg cttccgccga atcgaaagac
      421 gagaccgcaa ccaatgccag aaagaatacc tgtcatcaaa ctattgatca gtagactatc
      481 tacagcaaaa tgtatatgaa ggctctccca taaatgtaaa aagaaaacta gcgcaaatgt
      541 tccgacgatc gtccggagaa aagcgtgttt tccaaaaaga aacaatgaga taagcaataa
      601 aggtaaatta aaaagcagtg aagtgacaga aggagcgaat ccaaaaagat tatgcaaaag
      661 caaagcaata ccagtaaccc caccatctgc cagcttagat ggaatattga ttcctgtcac
      721 agctaatccg tagattcctg cgcctaaaat gattccgcac aattctgtaa ataaatttaa
      781 ttttttcatt tctagcctct tttcttttga aaattttcag caacttcttt attatactga
      841 cttatactct tacttcaaga ggaaaatgaa aaaaaattat ttttggctgt ttctttttat
      901 ttatcaaata aggagtggaa aaatgcgagc agcagactta ttgacaattc ttacaaaatc
      961 acccaaaaaa acagagtatc aaggagtttt tttggctcaa gaacaaaaac tggtgcctgt
     1021 gacaatggtc cagataaata aagcaaatca atttatcttt ttatttgaac acggcaaagc
     1081 accacttgct atgaaagaca tctttctttt tttgatgaag aaccgtgaga aagctgttta
     1141 ttatcagtca aaagaagaac tcattcctct ttatggagtg aaagagatag aaaataaagt
     1201 ggtgatataa gtaaaaataa gaaaacaaga cgtttatgat gtcaaaacaa ccatttttta
     1261 catttttggc aaaaaagtgt catgatacag tataatattc gttgaacagg ccaataaagg
     1321 tgttctcccc cacgagattt ctactttatt ggctgtttct ttttattaaa ataaaagttg
     1381 tgtgtgtaaa aaagatatag ctgattccaa gtgacaattt cgtatagatt tttatggtat
     1441 tcgtagtgaa aaaacgagaa ccaaaaagtt gatccgactt tgttgcttgg attctttttt
     1501 tgtcagcata tttagaggta tttttttctt ttttttgttc gtgctgtaag gttaaggcaa
     1561 gacaagttag ttgggaaaaa ggaaagaaat gtttatactt aaagaataac agaaaggaag
     1621 gtcgcatgaa taattttata caaacattaa gtgaaagaaa aggcgagttg ctggaagctg
     1681 catttcaaca tctgtccatt tctctcattt ctttgctgat tgcagcactg attgctattc
     1741 ctttagcgat ttgggcagcc aatcataaaa aaatggcaga agtcttattg cagatcacgg
     1801 gtgtgctcca aacgattcct tcacttgcct tattaggttt acttatccca tttgtaggga
     1861 tcggaactgt tccagcattg attgcacttg ttatttacgc tttgcttccg attttccaaa
     1921 acacgtatat tggtctagct gaaattgacc cgtctattga agaagcagcg gtagcatttg
     1981 gtatgtccag aatgagacgc ttgctgaaag tagagcttcc catcgcatta cctgtcatta
     2041 tctcaggtat ccgaactgcc ttagttttaa tcatcggtac ggctactttg gcagcattga
     2101 tcggagcagg tggattaggg acatttattt tgttaggtat tgaccgaaat acacctgttt
     2161 tgacattgat tggtgcgatc agttctgcct tgcttgcaat tatattcagc ggactgattc
     2221 gctggttgca gcataagaaa cctcgatatt ccatcattac tttagcagca gtggtgtgtt
     2281 tatttggcgg cgtttcttta tatcagtctg ccttgtttaa aaatgaaacc atcacgattg
     2341 ctggaaaatt aggagcggaa cctgaaatcc tgatccaaat gtataaagaa ttgattgaag
     2401 acaacacaaa aacagaagtt gaattaaaac cgaattttgg caagacgagt tttttgttca
     2461 atgcactgga tcaaggtcaa atcgatatct atcctgagtt cacaggtact gtcctagaaa
     2521 gcttggtgaa ggtacctgaa gcaatgaaaa gtgctgattt gacaaaagaa aaaacatatg
     2581 acgaagcaga agaattattg aataagcaat tcgatatgac attattaaaa ccaatggctt
     2641 accaaaatac gtatgccttg gcagtcaaga gatcttttgc gaacgaacat caacttaaaa
     2701 agatttccga tttggcagct atctctgatc agatcaaagc gggattcaca ctggaattta
     2761 ttgatcgttc agatggatat aaaggaatcc aagaagcata tggcttgaat ttttcaagtg
     2821 tacaaagcat ggagccaagt ttgcgttacc aagcaatcaa taatggagat gtcaatattg
     2881 tggatgccta ttccacagat agccagctca aggagtacga tctagtgact ctagaagatg
     2941 atcgagggtt attcccagct tatcaaggcg caccgctgat gaaaaaagat tttgctaaag
     3001 aacatcctga aatcgttaaa gcactgaata aattaagcgg aaaaataaca gaggatcaaa
     3061 tgattgatat gaattaccaa gtcaatgtcg aaaagaaaaa gccgacagaa gtagcacgaa
     3121 tcttcctgaa aaatgaagga ttagtaaagg aggaagatca gtgactaaat taatcgagtt
     3181 caaacatgtg caaaaaaagt atgatggaaa gtatgtcatc gatgacctaa atctggcaat
     3241 caaaaaagga gaaatctttg ttttggttgg tccttccggt agtggaaaaa caactacttt
     3301 gaaaatgatc aatggattat ctaaaccatc agcaggagac atctacttca aaggaaaatc
     3361 gttaaatgaa tataatctgc agaaaatgcg gtggaatatg ggatatgtgc tgcagcaaat
     3421 cgctttgttc ccgaccatga cagtcaagca aaatattgaa gtcatccctg aaatgttagg
     3481 ttgggaaaag cagaaaagag cagaccgtgt agatgaactt cttcaaaaag ttggcttatc
     3541 tcctgatatt tatcgagatc gtatgcctag agagttgtct ggaggagaac agcaacggat
     3601 cgggatcatc cgagctattg ctgcaagccc agatgtgatt ttgatggacg agccatttag
     3661 cgcattagat ccgatttcaa gaaactcatt acaagaattg gttttgtcct tacacgaaga
     3721 attgggcacg accatcgttt tcgttacgca caatatggaa gaagcaatca agctaggtga
     3781 tcgtattgca tttatgaagg atggagaaat tatccaatgt gacacaccag agcagttatt
     3841 gatgaatcct aaaaacgact acgttcggca tttctttgat gaaccgaaac agacgaaaga
     3901 gtggcgagta gaagatttag tcgtcaatgg atatttctta aatgaaatac cagaaaatgc
     3961 tcgtcaacaa gtttgctttg atacgcctat gaaagaagta tattcgcttc tttcggtgta
     4021 tccaagcatt gtgatagtag aaaagcaaag aagtattggg tcgctgacaa gtaaagaaat
     4081 ctttgctttt ttaagtcgag aggaggaagg aaatgaaaac atatgatgca atcatcatcg
     4141 gttcaggtgt gagcggctta agtgctgctt atggattgaa agaagcaggt aaaacagtac
     4201 tagtggtcga agaagatcta tggggcggga cttgtcctaa ccgtggatgt gatcctaaaa
     4261 aagtcttgtt aagtgcagta gaggcacgta atcgggtaaa acaattgagt ggaaaaggtt
     4321 ttaacgagat cccaactgcc aactgggaag aacttcaaaa attcaaacga acatttacgg
     4381 atccagtccc agaaagccga aaaaaacaat tagcagaagc tgaaatcgat catctttcag
     4441 gaaccgctcg ttttttggat gattcatcta ttgaagtcaa cgaagaagtc ttccatgcgg
     4501 attacttagt tttggcaacc gggcaaagac ctacgatctt accagtggag ggaaaggaat
     4561 acttgaaaac tagtgcagac tttttatcat tgcctgtact gcctaaagaa atcattttta
     4621 tcggtggtgg ttatattgct tttgagctag ctacgattgc caatgctgca gggagcaaag
     4681 taaccattgt ccatcataat caacggccat taaaagaatt tgaggcgtct ttggtagagg
     4741 aagccgtcca tcaaatggaa gcatctggca ttcagtttgc ttttggtgtt gaaacccaaa
     4801 agatcatttc agaaggtact cgctatcgtt tagtcggaaa agagacggaa ttagtagcag
     4861 atatgatttt ttgtgcgact gggagacagc cgaatacaga atctttagca cttgagcaag
     4921 cgaatatcgt ctttgataaa cacggaattg cagtcaatga ttatctgcaa acgagcaatc
     4981 caaaaatttt tgcgtgtgga gatattgttt caaggaaaac cccaaaattg acaccagtcg
     5041 ccacttttga aggaaattat gtagctaagc ggataacgga tgcaacaagc gaacctatca
     5101 aatatccgat tatacctacg atcgtctacg ccagtcctaa acttgctgaa gtaggagtta
     5161 caaaaagtca cgcctcttcg tcagatcaag tggtagagat ggatcttacc agttggttca
     5221 cctatcatcg tgtcaatgaa ccagttgcta aggctgaatt gacctttgac cagcaaaatt
     5281 atttgattgg tgctgcagtg attagcgagc aagcagacga attgatcgat gatttgacac
     5341 tcgtcatcaa tcaaaaattg actaaaaaag agttggatag ctatatcatg gggtatccga
     5401 cattggcaag tgatctctcc tatttattga aataatcagc atgataacta aagcgcttgt
     5461 tgctttttcc ttcttgtgct acactttaaa aaatgaatac ctgtttctgg ggatgtcgta
     5521 gacgacatga gagtaagttg tgaacttaga cccattgaac ctgtcagtta atactggtga
     5581 agggagaaac atagcagacg cttgaagcgg gttactatgt cgtgcccatt tcaagcgttt
     5641 tttgttattc atattagaag aaaggaggta acaaaatgaa gaaaaatctt tatccagcag
     5701 tcagtatggc attcatttta attatttggc agattgttgt catagtttgg aaaatacctg
     5761 cctttatttt acccggacct gctgctgtat tttctgcatt agcggcagat gcacaagtat
     5821 tactttccca ttctgtcacg acattaaatg aagcagtgct tggtttggta atagctagcg
     5881 ttctttcttt tattactgcc ttggcaatgg attattggaa gtttttagaa atgtcttttt
     5941 atccgttact agtgatttca caaatgttgc caatcatggt gttagggcca ttacttaccc
     6001 tatggtttgg tttcggtatg cttccaaaag tgatccttgt tgtcttgata agctattttc
     6061 cgatcgtagt ttctttttcg gatacattga ggaaagtatc aaaagagcaa atcatctttt
     6121 tgaaaacgat gggggcagat acagggaaga tttatcgaat atataaaata ccagcgggtt
     6181 tatccggctt cttttccggt ttaaaagtgg ctgctactta ttgtgtcagc ggagcagtcg
     6241 ttggagaatg gctaagcgct caagcaggct tagggtatta catgatacgc gtaaaaaaca
     6301 gctaccagat cgataaagtt tttgctgcga tcatttgtgt cattctattg agtttattgc
     6361 tgaatggcgc ttgttcatta ttgaaaaaag ggtattatcg tatgctttat attttttaag
     6421 agaaggggat caaatgaaaa aattatggct tttattaccg ctactgttgc ttttatccgc
     6481 ttgtggcact gcaaaagaaa caagtagcaa acaagaaata aaggatttga aaaaagtgac
     6541 acttgttttg gattatgtac cgaatactaa tcatactggc atctacctag caaaagaaaa
     6601 aggctattat aaagaggctg gtttaaatgt ccaaatcatc gaaccaggag ataattcgac
     6661 aagtattggt ctagtaggtg cggacaaagc acaatttggt gtaagttatc aagaagatgt
     6721 cacttatgct cacgcagatg gacagaatat accagtcaaa gcaattgcta cagtgatcaa
     6781 acataatact tctggattcg ccaccctatc agacagtaac atccattcac caaaagattt
     6841 tgaaggaaaa acatatgcag gttggcaaag tccaagtgaa gaagctgtct taaaagctgt
     6901 gatggaaaaa gacggcgggg acttttcaaa actgacaatg gtaggaagca atggagaagg
     6961 gccagaaagc ttaggaaaat cctcagatat ccaatggtac tttgaaggtt gggatatgat
     7021 caaagcaaaa gaagcgggta ttgaactaaa ctatatccca ttgaaagaat tagatgaacg
     7081 gttagattac tatactccgg taattattac gaatgatcaa ctgatcaaaa gtgatccaga
     7141 attggtacag tcttttatgg atgccacgaa aaaaggctat caggaagcta ttaaggatcc
     7201 aaatgacagt gctaaactgt tgcaaaaata tgcaaaagaa aatgatcgga cgtttttaga
     7261 agaatcacaa gcattcttgt caaaaaatta tacggatgat cctgagaatt ggggattgat
     7321 ggaagaaaaa gtttggcgca attatacagc ttttatgcag gaaaacggat tgatcaagca
     7381 agatgtacct tcgcaagaac tattcactaa tcagtttata aaataggagt gatagatatg
     7441 tcaatccaaa tcagccactt gaatgtagtc attgaaaacc tgaaagtatt agaagacatc
     7501 catttcacta tccctaaaga tacctttaca tcgattgtcg cgccaagcgg agccggtaag
     7561 tcaacattgc tgaaaacttt aacaggagtc cagcctatga cttctggaac gatcaaagta
     7621 gacgatcaaa aagtaactgg tctccataca gcatttagtt atatgccgca agaagatatg
     7681 ttacttcctt ggctgagcgt ctatcaaaac gttacgttat atcaaaaaat caatcatcta
     7741 acaatcaatg aagaacaagt aagggattat ctggatatct ttgggttaaa cggctatgaa
     7801 cattttttgc cggaacaatt atctggcggg atgaagcagc gaacggcctt gttacgaaca
     7861 atcatgaatc cttcttctta cttgcttctt gatgaaccat ttggagcgtt agatgctatg
     7921 accagagggc agatgcaaga ctggatgttg aagctgccta aaaaagcaaa gcggactact
     7981 ttacttgtga cgcatgatat cgaagaagca atttatttat cagacaggat tcttgtgctt
     8041 tccgcccgtc cggcacatgt gattgctgag attcaagtgc ctgaaaaacg acggtcacgt
     8101 gaatggctac tgcagcaatc agagttgaag caaacgattt atcagttatt ggcaggtgaa
     8161 ttagatgtta agtgatgctc attgtcattt agatgggagt cagagtttag cactccttca
     8221 gcaggaacac acgattttga cgatcatcaa ttgtgattcg ccagaagaat ggaaggaaaa
     8281 cagacagctt gcagcaagta agactcaagc cttaagttac ggtatccatc cttggaaagc
     8341 agatagctat acgtttgaac aagttgagcc ttttttgaaa aaagcaagga tcatcggtga
     8401 aatagggtta gataatattt ggacgaatgt accgatgact acacaaaaaa aagtatttga
     8461 gcgacagctt gcttttgcag ccataaatga gaaacctgtt gtactgcaca caaaaggatg
     8521 cgaaaaagag atcttaaatt atatccaagc ttaccctaat cactatttga tccattggta
     8581 ttcttctttg gactatcaac aagactatat tgatttaggc tgttatttca gcattggtct
     8641 tgatttgaaa aagaatccag cagtctggca actagctaaa gcagtcccaa tcgaccactt
     8701 acttattgaa acggatggac tacatgcagc agagtgggct ttagggggga ttttttcaga
     8761 aaaagattat gtctcactgc ttgaagaaca gttaaagaca atcgctgaga tcaaagagct
     8821 atcagttgaa gaagttaaaa aaaatactta tcaaaacttg gaacgtttca ttagattagg
     8881 taaataaaaa agagatgtga cacaactagt gtcgcacctc tttttggctt tctttgtata
     8941 aaagtaaact gtttgattaa taaatgaatc ttagtaccaa ccgtttgctt cccagaatgc
     9001 tttcgccgct tcccaagaac cataacgtga agtaacatat tgttctgcta ctctttcttg
     9061 gtttgcagca gagtagtcac catttaagta agaagaatct aattggtaac gtccgatgta
     9121 acgtccgttc gttgctgtgt aagaaccgct tgattctttt tgtgcgatcc attcttttgc
     9181 agagcttgtg cttgctggag tagcagcagt ttctgttaca gcaggagcag gttgtgtttc
     9241 agcaacaggc gctgcttgtt tttccgtttg aaccgtttct tgtacaacag gttgttcagc
     9301 ttggactggt gcttcttgaa cggctgcttg aggttcagca gctgcttgtg cagagccttc
     9361 tgttgggatt gttaattgtt gtcctgagta
//
LOCUS       DACECZ010000097         9386 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.097, whole genome shotgun sequence.
ACCESSION   DACECZ010000097 DACECZ010000000
VERSION     DACECZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.097"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            76..501
                     /locus_tag="IWO35_002143"
     CDS             76..501
                     /locus_tag="IWO35_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317193.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(989..1690)
                     /locus_tag="IWO35_002144"
     CDS             complement(989..1690)
                     /locus_tag="IWO35_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0317194.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(1706..2146)
                     /locus_tag="IWO35_002145"
     CDS             complement(1706..2146)
                     /locus_tag="IWO35_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0317195.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(2146..2721)
                     /locus_tag="IWO35_002146"
     CDS             complement(2146..2721)
                     /locus_tag="IWO35_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ0317196.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(3569..4048)
                     /locus_tag="IWO35_002147"
                     /pseudo
     CDS             complement(3569..4048)
                     /locus_tag="IWO35_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(4207..4908)
                     /locus_tag="IWO35_002148"
     CDS             complement(4207..4908)
                     /locus_tag="IWO35_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0317197.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(5016..6425)
                     /locus_tag="IWO35_002149"
     CDS             complement(5016..6425)
                     /locus_tag="IWO35_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ0317198.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(6473..8338)
                     /locus_tag="IWO35_002150"
     CDS             complement(6473..8338)
                     /locus_tag="IWO35_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0317199.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(8374..9333)
                     /locus_tag="IWO35_002151"
     CDS             complement(8374..9333)
                     /locus_tag="IWO35_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0317200.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
BASE COUNT         2738 a         2003 c         1575 g         3070 t
ORIGIN      
        1 ttttctcttc catccgtttg aagttttaca ttccaatggt ataatttctt tatcctatca
       61 aatgggggaa gtattttgaa gaaattaatc gtactattta ttttgtctgc gggacttttg
      121 acattgactg gctgtgattt gtttggcggc tctgaccaat cctcggacga aacaaccgat
      181 tcttctgtct taaaagcgga aaaagcagat accaccaaca gtgtatatac gctaaaagta
      241 cgtaaagata tggatttatc cgcgggacag ttgacattta cagataaaaa aatggaatgg
      301 acacgcacgt atcagacaca aggaactgat tcttctgaat catctgaaga aaaaacagtg
      361 ctgacagata tcaagatcac taccaaagat gacgaatata ttatttcagg aaaagaaaat
      421 aaaaaagaaa caacagtcat atttacaaaa atcggtaatt accgtttgaa ggatgaggat
      481 ggcaacatct acagtctata gtgccgtctg ttggatcttc ttttgtttct ccccttcttt
      541 tctgctatag taaagaagca agatgattgt aaaggagacg aaatggatga tcgatagtcc
      601 tgattattgt acatgtaata acaacaaaaa tatccataca gaatttaatg accaaggaag
      661 cttgagcata tgcgacaact gtcacaaact agtcaaagat tcatttaaaa catttgaatc
      721 ttatcctgtt caaaattatg taaaaagtag aagaagataa acaaatccaa gcaaaactca
      781 ctatggctga tttttatgaa cttcaaactc agcttcttcc tttgatcaag cttcatcagt
      841 aaaacaaaca gtggatcatt tcagtttaca aatcaaagag taaagattta tttatttcaa
      901 atatttgaat ataaaataac caaacgatag aaaggcttat tctacatcac gtagtaattt
      961 ttctttcccg tcgtttggtt ttttattttt agcctaattc ttgacctttt gcatgctggt
     1021 tatccgtaaa atattttcca gcaggctttc cttccaattc agctaatttt tcttggatct
     1081 gacggatcac cttttttgca tcttctggat tttctacaaa atcgaattta tccccatcca
     1141 cttgtatttt aggacataca tcaaaatctt cataccattg attatagcgg gtgttcaacg
     1201 tttcataata ggtatataac gtctcatcgc tttctagctg ttcatagtca cgcccacgtt
     1261 ttttgatacg ttctagcatc gtgtccaaag aaactttgat atggaccatc aaatcaggcc
     1321 gtttttttgg agcaacatca tctaactcgt ttagcatcgt atctaataag ctatcgtact
     1381 gctgtacttc gatatccgtt acccgaccta aatcagcgtt tagatggaac aacaggctat
     1441 cttcatagat ggatcggtct agcacattgt catcgttcac taaagcattt ttcattgcta
     1501 aaaatcgctt gttcaaaaag aagatttgca ataaaaaagc gtactgctca ggatttgaat
     1561 aaaagagcgg caatacttcg ttatcttcga cattttcata aaatggttta gaatcaatct
     1621 cttttgcaag catatccgtc aaactgctct tacctgcacc aatcgttcct gaaatcaaaa
     1681 tcactgacat acaacccttc ctcttctata agtactcgcc tacgtaagtt tcaacaggca
     1741 atgtttcaaa atcatattca cgtacagctt ggtctgattt taaatacgta tggagacttt
     1801 gtgtaatcat caagttcgta ggtaccgtct cttcttgtac aatgatcatc ggttttttca
     1861 acatagttgc cacacctaat tcgtaagccg ttcctggatc gattgtttga tgctcaaagt
     1921 caaggatagc aacaacaaat tctgcatttg tcagttcttc catatctttt tcgtatactt
     1981 cctgcgccca tccagcgctg aatagttcat aattgctttc ttggtgatga cgtggagagt
     2041 agaaacttgt gactgttttg ttttcctcca acgctttttc gatacgactt actcgatcga
     2101 tttgctcctc tgaaaaaaat ggtcctgcta aatatatttg actcattatt ttgcctcctt
     2161 caataataac tgatttttaa tagatactgc tcgtaattca ttgaccagtt ccaatggatt
     2221 gttggaaaag attacttgat ccaaccattt tttatccata aaatcctctt ttgcaaaagt
     2281 ttctaataat tgttgcaagc cgctgtaata accgccaata ttcaatattc cgattggctt
     2341 gtcgggcgtc agaccgattg ctatccaaga aagaagttgt gagaattcct ccaatgtacc
     2401 tagacctcca gggaaaacga ggacagcatc tcccttttca aataataata cctttctttc
     2461 atcgatcgtt tctgtaggaa taaatgtagt aacatctttt ccaggcaatt cagtttcaaa
     2521 aagcccattc ggatagatac ctgtgacttt tccgttattt tccagtactg ttttactaat
     2581 cactcccata atcccggagt atgaaccacc gtaaaccagt tcgatttctt cttgtgccat
     2641 gtattttcct aacgtttgcg caataaattt gtaactttct ttattaccaa aacgggaacc
     2701 gcaaaaaaca gtaagtttca taaaaaaaca ctccctaact caaaagtcta tcttcattct
     2761 acacgtgttt ttttaaaata aaagactaga ttttgtagtt ttttagtggt atataggata
     2821 gacgccacta tatatagtat ttagaaaaaa atcatttttc agccatttta tagataaaac
     2881 aaaaataaat aacgtgtttt catcgatatg aaatgactta cttatagaag atactgggat
     2941 ttcgtcctat atgccaataa cgtgtattat tttaaacaac aaatacttgt ttcttttatt
     3001 aacgaaaaaa tcattttgtt ctcgtgttat gtttcactga taaatgatgt ttctctaaat
     3061 aaaaaagccg acgaactaaa cgatcaaaat ttgatcattt ttgttcatca gcttctccta
     3121 aaaatctttt tgttaacgat aaatagctgg atttgtaaaa atatcacttg aatcgtcact
     3181 gctggaagaa aattcagtga taaccgcacc tgttgcgcct gcctggaact agtgtttgat
     3241 attgggctcg aacgtgtact gatctctagc atgaagaata atttcttttt ttactgtata
     3301 ataactttct ttcctttttg gaatagtaca ggaaagtttt tttgtttcct ttccttcgac
     3361 ataaggtata ccagaccctt tcgacaacga aaggttctca tttttccttc atcgtcattt
     3421 caaggagggt gccgatgctc tggacagttt cgatcgctcc taaaccaaaa tctgtatatt
     3481 cgattctttc taactctgat tctttcatac cattcctgtc tcaaggtaca tctattgtac
     3541 ttttttcttg taatccattc tgcctctcct aactaattgt caaatcatcc caagatatgt
     3601 cttcttcctc tttttcttct tggatgattt ctgcattcgg atctactggt tttttcaata
     3661 ataaaatggt tattcctgtg acaaaagaac cagctaacaa tgcaccaatg aatgcccatg
     3721 gtctggtcat cgtagcgact gcaaacattc ccgatgccgg aatacggcta tctgcgcccc
     3781 aaaacatgct taatccgcca cctactgtcc caccgactcc cgttgctatg acattgcgtg
     3841 ccaagtcgtt caatacgata gggaaacatc cctctgtgat ctgacagatc cccataggaa
     3901 aagcggtctt caatgtatcg atttcttgac gattgagtat atttttacga atgattttcc
     3961 ctaagaaata agcgatcgtc aagccgaatg gggttaccat cgaagctaaa atcaatacag
     4021 tgattggttc atagattcct tctgaaaatc gtgccacacc cccttatcca gaataaatgg
     4081 taatccagaa acagccgtgt atctgcggta ataagcctaa ccaaacgaaa aatatgagaa
     4141 gctatttttg tttggctgtt cttattactt gatgcagggc agctttttca caacctcttt
     4201 ttttatttat gtttcataat caatatggta aggatgcagc tccgtatggt agaccgcctc
     4261 cgaatattca atcatttccc caatttgcgt agacgatttc cgtatccgtt tgatgccgat
     4321 ctgtgcgtct gttttcagaa ttccttgttc ttcttctgtt aaaaatactg ccgagaaatc
     4381 atcagaaaat gagtcaatag tatatccttt tttcgtcagc aaacgataca aagattcttt
     4441 gccaaattct tctctgctga attgcatcat ttcttgtgga agataatgaa caaaataaat
     4501 ataaggctga ttgttcaaat aatataagcg cttaaaacaa acagcttgct caccgaaaaa
     4561 ggaaaacaaa ggatcttcct tctttatatg gaccaattgc aaatccagcg tttctttccg
     4621 gacttcatat cgggagttct ccaagatttg cgtaaagctt gccgctttgg ataatcgatt
     4681 gtaaggacga ttgcttagaa ccgtcgtccc acgaccactt ttctttgcga catattcatc
     4741 tgatgccagc agttcgattg cctttcggac agtgattttg ctgacttgaa acagttcttc
     4801 taattcactt tctgtcggca acatcgaacc gactggatat tttcctgaga aaatatcttc
     4861 tttaatttta tctgcgactt ccaagtatag tacacgtgtc cttttcataa cgtcaccctt
     4921 tgctttacta ttactaaaag aatagcacat aaaaaataaa aaaagaacac aaatcacatg
     4981 tgagaagtgt gttctttttc attcagctgc tttaattaca agtttacgcc atttgaggca
     5041 attacttctt tataccaatc aaaagatttt ttctttgatc gattcagtgt cccatttcct
     5101 tcatcgtcta gatccacata gataaagccg taacgtttac tcatttctcc tgttgatgca
     5161 ctgacaagat cgatacatcc ccaaggcgta tagcccatca aatctacacc gtctgctact
     5221 gcattagcca tcgcttcgat atgacgacgt aaataatcga tccgataatc atcaataatc
     5281 gtgccatcct cttccatatg atcaatggct ccaagaccgt tttcaacaat gaataatggt
     5341 ttctggtaac gattgtataa ttcattcaat gcgattctca gcccttctgg atcgatctgc
     5401 cagccccatt cacttgcctc aagaaacgga ttttttactc ctcccagcaa gttcccttct
     5461 gaagttgctg cttgtggatc agtctcattg attgccgtag acatataata actaaatccg
     5521 atgtagtcca ctggatattc ttttagcaac tggaagtctt tttccgtttg ctctaatttt
     5581 tccggatcga ctccatattt tttatgggta cgcgctgtgt atgcagggta ttctccacgt
     5641 acttggacat ccgtgcagaa aaaattgaat tcttggtttt ggatcattgt tgctgcctga
     5701 ttgactggat tggcatcgat gctgtaagtt gttgcataga taatcatgca gccgatttgg
     5761 atatccgaac gcaattcgtg gccgattttt acggctttgc tacttgcaac gaattgatta
     5821 tgccatgctt ggaaaatatt ttggtactct cctgctccat tgctttttac taatccttga
     5881 cttaaagctg gaaagtgaaa ggccgaattg atttcattga aagtcatcca atatttgact
     5941 ttactgctgt atctctctaa tactgtctga gcaaatcgtt catagaagtc gatcagtttt
     6001 cgatttttcc agccaccgta ttcttttgcc aaatgaagag gcatttcgta atgagaaatc
     6061 gtgatcacag gttcaatatc atattttaag cactcatcaa tcagctgatc ataaaattct
     6121 aatcccgctt cgttaggagt accttcgtct ccatttggaa aaattcgtgt ccaggcaatc
     6181 gaaaaacgat aacatttgaa tcccatttcc gcaaacaagg caagatcttc tttgaaacga
     6241 tcgtaatgat cgattcctcg atgattcggg taaatatatt tttcttgatc aatcgtccag
     6301 tcaaattctt cactgccgat aatcgcaaag cgctgcttac ctccaggcat agcatctgca
     6361 acggatagtc cttttccgtc tcgatcataa gccccttcta attgatttgg cagcagttgc
     6421 tccaccccat aaaaattttt ctgaaaaacg tgattgcatc ctgatttcct ccttatttta
     6481 caattgccag aacatcttcg tttgcaataa tctctttttg gtctaattca agtatatcgg
     6541 caaatgcatc agtattcgta atgacaactg gagtgattac gctatagcca gctgctctaa
     6601 ttgcttcgat gtcaaaacga atcaataaat ctcctgcttt gacgagatcc ccttgtttga
     6661 ctaagatctc aaagccattc ccatttaatt ctactgtatc catcccgata tgaatcaaaa
     6721 tctccgctcc gtcagtcgtt gtgatcccta cagcatgtcc agttggaaaa atagtcgtga
     6781 tctctccgtc tgcaggtgca actagttctc ctacttctgg atcaatagcg ataccttttc
     6841 ccatcacgcc agaagaaaaa acttggtctg gtacttcact caatggcagt actttacccg
     6901 ttaaaggact ttttaaggta tgccgtgctg aagtaatcgg ttctcctgca ttcgcatgtt
     6961 tgttttctaa ttgattgtct tgtgtttgtt cgtcaaatcc taaaatcaat gtacctacga
     7021 atgcaagaac aaaagcaatt cctgtagcta ttactgctac tgtcacattt gactctacac
     7081 catccaccgt attaatgaaa gtcggaatcg tcaataagct gaccaaactg ctggagaaag
     7141 cttttactcc ggaaaatccg ataatagctc caccaatacc tccagaaata caagcggcaa
     7201 tgaatggttt cttcaaaggt aatgtcacac catacacagt tggttcagtg attccaaata
     7261 acgaagtcac tgtagaagaa atccctaatg ctcgaagttt cgtatctttt gttcgcaatg
     7321 ctacagctaa agcagcccca ccttgtgcca agatggctgg aagaagcatt ggcatcaata
     7381 catcaaaccc atattgttcg atattcaaaa acatgatcgg tacgaatccc caatgcatgc
     7441 cgaacatcac gaatacttgc cataaacttc ccatgattag acctgctacg attggactaa
     7501 atccataaat cgagttgaag agaccgccta aagcattacc ggcaaccgtc ccgattggtc
     7561 ctaaagcaag aaatgttaat ggaaccatca ctactaaaac gacaagcgga actaaaatca
     7621 tttgcagaaa ttgcggaatc acttttttta cgaatggctc taatttactt tgaacccata
     7681 cggctagaat gattggtatg acagaagaag tataaccaga tggtgaaaga atcactggga
     7741 tcccaaagaa actgatagct tctcccgcac cagcaactgc tgtgatagct ggatacacaa
     7801 gtgccatagc aattgctact gctaaaaact ggttggcttt gaacttttta gctgctgtaa
     7861 aagcaagcat gacaggtaag aaggtaaaga caccatctgc tgcagcaaac aatatttgat
     7921 aagtaccgct atcagctgtc agccagccca tcaccgttgc caatgataag aatcctttca
     7981 ataccccggc ggctgccatc gcccctagaa aaggtgtaaa tatactagag ataatatcga
     8041 tcagacgatt caaaatattt ttggactctt cttttccatc ctccgaaact gcaagacctt
     8101 cctcatccac gatcgattga taaacatctg ctacattact gccaatcact acttgatact
     8161 gaccgccact ttgtacgact tggatcacgt ccgggtcttc ttttaacttt tgcgtatctg
     8221 ctaaagattc atcttttaat ctgaatcgca accgcgtagc acaatgtact aaacttttga
     8281 cattcttttg tcctcctacc gcttcccata cacgacgacc aactgcttga ttatccattg
     8341 ttttttcctt ctttcttttt tatttttcgt ttgtcaaatt aagttagaca aatcatcttt
     8401 tgtgacgtaa ctcaaaaata cttccattgg tgtccgatag tgtaatgatt ttctaggaat
     8461 atgatttcgt ttcgatgcca ccgattggat aaaggtttca gatacaccat taaaatccat
     8521 agctttatac aaaccatctt tacgtaataa gccatttgaa tgttcattca atcctcgttg
     8581 agaaggtgtt cctggatccg caaaataaat tgaaatatcg ttggtattac taatcttttt
     8641 ccaatttgag aattcttttc cacaatcaaa tgtgatggat ttaaataagt tttttgggac
     8701 acaacgaaac cattgattca gcctattttc aatgtcttta gcctgtctgc cttcgggttt
     8761 caaggtaatg atgactttag atagccgttc aactaatgtg atgaccgcgc ttttatgttt
     8821 tccacccacg atagtgtcac cttccaggtg accaaattca tgatcaaact gggtatagtc
     8881 tgtctttctg tctcggattg accggcgaaa cccttgtttt cctctttttt ctttatgacc
     8941 gtttggtttt ctttttccct tcattggtaa atcgtgagtg tcaaaggttt tatctttaaa
     9001 cattcgatac agtgtgcgta ccgaacaagc aataggaaac tctgctcgac caataaggac
     9061 atcaggagcc cacccttgaa caaccttttt ttgaatgtat tcctgttgct catcaggtaa
     9121 aacaatcgga tgtcttccgc agttacgttt gtttttctta tattgtttat agtagctcat
     9181 tgcgcttcca ccagatttaa gaaaacgata gaccttataa atagtttgtc gagaacgttg
     9241 taacagattg gctacttttg aaacaggttg tgattgattg aaataagctt ctatcattac
     9301 gagttcagtt ggtgtaagat ggttataggt catttgtact cactccttat aattctttcg
     9361 tcggaactat tttgagtgta gcacaa
//
LOCUS       DACECZ010000098         9303 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.098, whole genome shotgun sequence.
ACCESSION   DACECZ010000098 DACECZ010000000
VERSION     DACECZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.098"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            70..264
                     /locus_tag="IWO35_002152"
     CDS             70..264
                     /locus_tag="IWO35_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317201.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            533..832
                     /locus_tag="IWO35_002153"
     CDS             533..832
                     /locus_tag="IWO35_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317202.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(929..1363)
                     /locus_tag="IWO35_002154"
     CDS             complement(929..1363)
                     /locus_tag="IWO35_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0317203.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1595..2305)
                     /locus_tag="IWO35_002155"
     CDS             complement(1595..2305)
                     /locus_tag="IWO35_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0317204.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2295..3155)
                     /locus_tag="IWO35_002156"
     CDS             complement(2295..3155)
                     /locus_tag="IWO35_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ0317205.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3160..3798)
                     /locus_tag="IWO35_002157"
     CDS             complement(3160..3798)
                     /locus_tag="IWO35_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ0317206.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3813..4112)
                     /locus_tag="IWO35_002158"
     CDS             complement(3813..4112)
                     /locus_tag="IWO35_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0317207.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4155..5639)
                     /locus_tag="IWO35_002159"
     CDS             complement(4155..5639)
                     /locus_tag="IWO35_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0317208.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5659..6120)
                     /locus_tag="IWO35_002160"
     CDS             complement(5659..6120)
                     /locus_tag="IWO35_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0317209.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6138..7205)
                     /gene="ulaG"
                     /locus_tag="IWO35_002161"
     CDS             complement(6138..7205)
                     /gene="ulaG"
                     /locus_tag="IWO35_002161"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ0317210.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7423..8193
                     /locus_tag="IWO35_002162"
     CDS             7423..8193
                     /locus_tag="IWO35_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0317211.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8466..9293)
                     /locus_tag="IWO35_002163"
     CDS             complement(8466..9293)
                     /locus_tag="IWO35_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0317212.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2879 a         1915 c         1473 g         3036 t
ORIGIN      
        1 gatacataat cattttcact tacatatatt tgaatattaa ctaaaaacag caagaaagaa
       61 gaggatcaaa tgtattatgt agaagtaaaa acaaaaggcg tcaaaaacaa acaatatgtg
      121 aaaggtatca gtaacgaata tcctttactt ggttcatgga aagaagctgc tcctttttcc
      181 aaaccatgtg ccataaaaat aaagaacgag ctagaaaaag aactgacttg tggtaaagct
      241 gttgtagata tcatcgaaaa gtaaatccaa tgtatagaaa ggaagatcac acatgaaaag
      301 cgacgtacaa attgctaaga ttatagaatt agaagaagaa gacgcagtgt tgacaattga
      361 aaaaaaataa atgtttgtat accatatgat tatttcaata tgtatcctgg aattggagat
      421 ctcgtagagc tatatcaaga tgaatcaaat atacttgtca ttcatcatga aatatagaga
      481 cttataaaat tgtttatttt tataatgtat cagtatagaa aggaaataaa acatggatta
      541 tactgttgaa tttgacagtg aactacttaa gcaatttcca caagaagaac atttcatgta
      601 taaagaagca atttcccaca aagtgacaga gtatttggag cataattttt ttcgtataaa
      661 gccagtgcgt actcgttcgg caaaaggaat atacgagatg aaagtcaaaa ttaataaaaa
      721 aaattatcgc atagcatttg ctttaaaaaa aaatcatgtg ttcatttttt atattagttc
      781 gaatttgcaa aaaattgtat ttgataaaga ggtgagtaaa aaaataaaat agctgagcat
      841 tggaggatag attgagtgat actaagtcta tcctcttgtt tttctaaaga actaagctag
      901 aattttttct tttgtacgtt attatatgtt atttgtcatt accaaaaagc accttttttc
      961 ttctagtatc tatgacataa gaaataccac ccatttcctt tacttgaaca aatcctactg
     1021 attcataaaa tttttttgtt ttctcagtat catctgtcaa taattcgatt tggactggtc
     1081 cataactagt gatcttctcc aaagcttcat tcaaaagcac tttgcctatt ccttggcgct
     1141 gatacctcgg atgaatgagg atatcttgaa taaatgcgat cgtcgcaaaa tcagtgatcc
     1201 aacgaacgat tccgataatc ttcccttccc cattacgctt tatcagagat tcagattgtt
     1261 caaatgctct agctaacctt gcagtgtctt ttgtgtaatt cgtccaacca acgctcttat
     1321 ataattcaga aatctcatcg atcataaggg aatcaatatt catttttttc ttcctttctt
     1381 atacaattct ccctctaata atacagttct ttgactattt aatctatcac atataactct
     1441 cctgtaaaga ttctctcgat tccttaaaat tcctctttcc ttttagaatg catacttttt
     1501 gataaataaa aaggttgtga taaattttct tcacaacctc tgtttctctc tatcaagtgt
     1561 tatcttttaa ttttctaccc ttaccctagc atctctattg cccgtagtat gcatactttc
     1621 catgtttgcg aaaataatgt ttatctagta aatattgcgg gatttcacat atattggggt
     1681 tgatcagctc gttttctttg gccatcagac aaatttcatc taagatcaaa ctgttttcta
     1741 ctgctttcat aggtgaatcc ccccatgtga acggtccatg tccatagaca agtactccag
     1801 ggacttcgtt tggatctaat tttctttcct taaacgtctc gacaatcaca ttccctgtat
     1861 gaacttcgta agcttctctt acttcttctt tcgttaattg gcgtgtacaa ggaaccttac
     1921 cataaaaagc atcagcatga gtcgtgccat aagctggcaa atcgcgtcct gcttgcgccc
     1981 acatgacgga gtgcgtggaa tgagtatgag taatagcagt tatatcttca aaagtttgat
     2041 acaaaacaac atgtgtgggg agatctgaag atggttttaa gctatcttct tctattatat
     2101 ttcccgacaa atctgttacc accatttgat ctgcttgcat gcattcatat ctgacaccgc
     2161 ttggcttaat gacgatgact cctagctcac ggtccacttc actcacattg ccccaagtca
     2221 atttgactaa tccgtatcgc ggcaatgcca gatttgcttc aaggacacgt tgcttcattg
     2281 ctgcaatttt ttcttcacaa gacatcatag ccagcctcct tcaatttaga acttaagtag
     2341 tcttttgcct gctggatttc tcttcgaaag tcttggcttt tttcactcca catttcaatc
     2401 agaaatgttc catcataatc tagacgtttc aaatgtctta ataatccaag aaaatcaaca
     2461 caaccgctac caaaaggaac atcacgaaac tggcctgaat attcttttgt tacagggtaa
     2521 gtatctttta agtggatagc agtgatataa tcgatccctt tctcaagttc tcgagccgga
     2581 ttattttccg gccatgcgga caaattgcct aggtctggat atacttgtag aaaaggtgaa
     2641 tgaatctgtt ctttgatttc taaaaatttt gaaatggagt tcataaatgg atcatccatg
     2701 atttccacgg aaagaatgat gccatattta gaagcttcct ttacgctttc ttttattcct
     2761 tcgataaagt cttctctgga attcattgat ttctcttcat aataaacatc gtatccagct
     2821 atttggatta ctttgacgct tagattatgc gccaaatgga ttgctttttt cattagttgt
     2881 ttcgctgctt ttttcttttc tggatcagca gaaccaaacg gaaatcgccg atgcgcactt
     2941 aaacaaatcg aatggatacg tacaccggag ctgaacatct cttctctcag ctcagctatc
     3001 ttttcttcag accaatctaa tcgtgctaac cgctcatccg tctcatcaat cgacatctcg
     3061 ataaaatcga agtccatttc ttttacaagg gaaaaacgtt ctttccatga aatatctttg
     3121 ggtaacgctt tttcataaat cccaatttga gccatttcct tacccccaga tacgaatgat
     3181 ctcatctttg aagttttgtg cggccttcat cggatcattt gctgctgtaa taccacgacc
     3241 agtgataaag gtatacacat cgatcccttc aaataagtgt aatgtgtgtg gatttaatcc
     3301 accagttaca gaaacattaa agcctagttc aatcagtttt ttgatcttcg ataaatcttt
     3361 ttcaccccaa gtttcaccag ataacagcgc atcacggctt tgatggtaga ttacctgccg
     3421 gataccgatg ttatgccaat ccatggcttg ttcaaaagtc caattgccgt agagttctac
     3481 ttgcaattca cctatttctt tttgtgctgc ctccatagtt ggaatcgttg cacaacaaat
     3541 gactgtcatc cagtctgctc ctgcttttgc aacattactc gccactgttc ctccagcgtc
     3601 tgcacatttc gtatcagcga ctagtttttt atctgggaac atactgcgaa tacagcgaat
     3661 cgctttttgt ccttcctgta agcaaagaat tgtgcccact tcaataatat ctactacatc
     3721 accaactttc ataacatctc ctaatgcatg ttctaagtca ttgtgatcta acgcaatttg
     3781 caaattcggc ctgctcatct tagttccttc ctttacttgt ttttttggta ccactttgtt
     3841 tcagcaaatt tttcactgat ttctttgact gacatcacat ttttgatacc gataactgta
     3901 actccttttt tctctgcctg agtaaacata ttcaaaaaat tgactggcgt aaaaactaca
     3961 tcgtattggc cagctgaact ttttccctct gaaatggcac aatgatggat tttggtaatt
     4021 ggtacgttta gttctttcat tgctttttcc acacttctca tcatcatcaa gcttgttcct
     4081 gatccatttg cacacgatac taaaattctc atcttaatcc ctccattatg agtgttgtct
     4141 aatcagattt tcttttatac tctctgcttt tctgcatatg tgtcatagtc ttctacaatc
     4201 aaaaaataac catcaggatt tttccgatat tgtaactgcg gaatagccaa taaaccaatg
     4261 acaactacgg caattccagc ataacctagg actttcatga ttactgtaaa caatggccaa
     4321 gctgttgccc agtcgaacat cccgatataa ccaccgtatg ttgacatccc aatccatgta
     4381 gcgaataatg ctgaaccgcc tacttgtatg attcctgaaa gaaacgggaa caggcaagcg
     4441 gctttgaatc cacctcggtt attggcataa actgctataa cggcattatc aaagaacaac
     4501 ggaatgaaac cggctatgac gatcgttggt gatttcaata aaatcagtaa accaatcatc
     4561 aaaaactgtc ccaaagcacc aaacaagaac ccaatcgtta ccgcatttgg cgaaccaaag
     4621 ccaaaagtag cagctacgtc aattcctgga acagctcctg gtaaaatcgt gtttgagatt
     4681 ccttggaaag attgagttag ttcatcgacg aatgtccgaa cacctaattg caagatcgcc
     4741 agatataccg caaaatttaa agaagtcgtc ataatgtaga ataagaaact ctgcccttct
     4801 tgcatgaatt gcgcttgaat caaataatct ttgcctagta caatcagaat gatcccaaag
     4861 aaaaatagca tgagtaacga agtagcaacc atattctcat tgaaaatcga caagaatcct
     4921 ggtaatttga tatcttcaat ttttttgtct tgcttgattg atctattttt cttttcatcc
     4981 cgctttttca tccactcagc taattttgca aatataaaga ttccgaacat ttgctgatgt
     5041 gccactgcaa atccggcacc ttctgtcaat tcctgcgtga ttcctactgt taagttagaa
     5101 gctactgtcc aataacaacc taaaatcagt cccataaaaa tcaatgcttg tatcctacct
     5161 agatcaggaa agcaaaacag taaaatccaa aaagcggtag ctgcttgttg aacctgaacg
     5221 tttccggtag tgaaaacgga acgcagtttc gtatatttct gaaaacggac taataagata
     5281 tttaaaacaa atgcaatcag taataaaatc atcacatctc caaaggtacg gccaaatgtc
     5341 tccattaatc cagcatccac agcattttgt ccaaaataag gatctgtaac catggcatcc
     5401 aaattgaacc gttcctttag tccaaccaag attggccgaa aattattgac caaaccacca
     5461 gagccaacag tcaaaataaa gtaaccgacc gttgccttta agaatccagc gatgcattca
     5521 tgaagcgggc gtttcagcaa aatatagcct aataacacga taaaaccaat caggtatgct
     5581 ggctgcgtca agatatttgt tgcaaaatac gtccaaatgc ttaacaaaaa ttcccccatc
     5641 ttttttctcc tctatcaatt aattatattc tgccatcaca acttggtaat catcgatgga
     5701 gttcgtagag atgagacgct caaccaatcc atctgtcatt aataattcgg ataatttttg
     5761 gatattttct agatgttctt ctggatcttt tgctgcgagt gtaaaaaaca aactagccgt
     5821 tttctcctca ttctcccctt cactttcaaa acggattggc tgtttcaact tggtaaatga
     5881 aatagctgtt ccaaaaacac catcactttt ttctgaagaa tgcggcattg ctacttgagg
     5941 tgcgatcaca atataaggac cgtatttttc tacacaatgg atgatctcct ccacatacgt
     6001 ttcatcaata atttccttat cgatcaatac aagacagctt tcggcgatag cttcacgcca
     6061 atccgtgatt tctttatctg ggtaatgaat caattgatgg tcaaaaaagt atttcagcaa
     6121 gaaactcccc ttctttctta taaaaacgac ggaaacggtg tatcattttc aatcgagaag
     6181 acatcttcaa atcctcgata aaaattaaac tcaagatcat ctttgtttgt cggatagatg
     6241 aatttcccgc ctacttgcca gatataaggt ttaaatccat acttcaggcg atcctttttg
     6301 aatttccaaa tttcagtgat ctcttttgga tcagccataa aattagacca aatatcgtag
     6361 tggacaggaa tcactacttt tgcattcaat gactccgcca tgcgcagcat atcaacagaa
     6421 gtaactttgt ctgttatccc tcgtgggttt tcaccatagg cccctaaaca gacatcgatt
     6481 ttgtgttcat taccatgttt ggcaaataaa ttggagtaat gtgaatctcc tgcatgataa
     6541 agattccctc ctgatgtttc gaataaatag tttactgcaa tttgatccat ctcctgaggc
     6601 atttttcctt ttaatacctc ttctggatct tcacaagtga ccaacgctgt ccgatcaaac
     6661 gcttctaaag ctactacatt gatgtctttg attttgactg catcacctgg ctttactgta
     6721 atcgtttttt cttcaggaat tccccatttt tgccacgtgc gaaccacttc ttttggccca
     6781 atgaatgttg cttcaggaca attttgatga actgctgcgg ctgtatggat atctagatga
     6841 tcggaatgga tatgtgtaac gattaaagcg tctacatttt ttatcgcaaa aggatcgatt
     6901 acgaaaggct gagtacgtaa attaggctgc atattttgaa cgccactcat acgcatcatc
     6961 tgatggcctt ttttcatcat gccattccca tgactacgtt ttcccgtccc acaccataga
     7021 tcacataaaa tattcgttcc ttcatgactt ttcagccaaa ttcccgtaca gcccagccac
     7081 cacatagcaa aagttccagg atccactact tctgtttcga tttcttcatt caagtatgtg
     7141 ccccactcag gaaatgtttc tactaaccac ttttcttttg ttacttcatt gacagtttta
     7201 ctcatttgct tgctcccccc atatcgttgt tccaacttac aagtaaatat taatgtgtat
     7261 cagcatccat gaaaaccctt ttatatgacc gtttgttttt tatatgattt tctttcagta
     7321 atcaatgatt gtttcagtat aataaagaca gatatgaatg tttaaatgaa caactagtca
     7381 aataaatcca tggatcaaga tgtccctcag gaggcaaaag tcatgaaaaa ctcacttgtc
     7441 aatattgaaa aacgtcaaaa agaaattttg gaactgcttc agcagactga acaactatca
     7501 actttcgaat tagctgaatc ccttgatgta tctattagta cgatacgaag agatttaaat
     7561 ttattagaag aaaaaaacga gatcatacgc aattacggtt attgttctta taattacaaa
     7621 aaccagactg attttgatca atctggtcct gaaaggatta aacaagcgat tgccagagaa
     7681 gctagtaaat acattaacga ttatgatacg ctgtttatca attctagctc gactgcgttg
     7741 aaaacactgg attttcttca atctaaccat ttgacagtca ttacaaacaa cgtgaaaatc
     7801 aattactctc cgcacaaaga taattgtagt tatattttaa ctggtggaga gatgcgcttt
     7861 ccaaaagaag cactagtggg cgacatcgca ttaaatacga tttcttcagt caatgcagac
     7921 atttgtatca ttggctgtag cggagtcgat gtagtacatg gggtcacaac aaagatatta
     7981 aatgaatcaa cgatcaacga aatgatggtc aacaagacaa tcaaatgtaa gattttagtc
     8041 gctgatcatc gaaaaatcgg actgacatca aagttcaaga ttgctgatat ttcagtcttt
     8101 gattatttga tcactgataa attttgttct gaaaaagtat tagaggagat cagaaatacg
     8161 gggataaaaa ccattcaagt agattcattt taagaaccag gacgtaaata tgtaaatagg
     8221 attatcaaaa aaatgaataa caaatttatc gataaatgaa aaacacgcta cgctcaaatt
     8281 gataaaggaa tcccaagatg tatccatcca atcgatacca tcataatcta ttgcttcttt
     8341 tataaccgtt atatgttgcc taaataggaa tcaaacaata tcgaaaaaag gaggtatgac
     8401 aaaagtcaca cctccttttt tcgaataaac ggtattcaaa tcagaacact gatttcacaa
     8461 ccactttatt tcaggtcgtt ttcaatcacc tttaccgcgt aatcatttcc gttgaaacgc
     8521 gctatttttt ttgcgtccat atatctttct aaataatcat cttctcctaa ttgcttatgg
     8581 caagaagcaa tttcataaag caagtattcc aatgcataag tcgatttatg tttcttatta
     8641 tactcaatac ccttctcaca taattggatt gctttatcat aatcctcgat tttacggtaa
     8701 taggtggcga tatcataata tacaatgcag accatcagat tatcaaaatc agtctcactc
     8761 aatattttaa gtgtacgctc cacataaact ttcgcaaaat ctaattctcc ttttgacaag
     8821 tagatcattg ccacgtttgc agctgcttgt aagtgatgga tatctgtttc ggtcgtttgt
     8881 tccaacactt gattaaaata aaataatgct gcatctggtt ttttcgcata aagatgatca
     8941 agcagtcctt gataaaataa aatttgattt aaaagatcag aattacttac tttttcctga
     9001 tcgatcgttt gtaaaatttt ataagattct tggtattttg atacagaatt caatgattga
     9061 atctcattta tttttttcct gattttgtct tcttctgttt ctactaatat atcggagatc
     9121 tcgacattca gctttttgca aagcgcactt aagatattga tgcctggcga catattcttg
     9181 ttttcgatat tgctgacagt tgcttgttca caaatgcctt ctgctaattg tacttgtgtt
     9241 aacccctttt tctttcggta ttcttttagt aatgctccat ctactttaat caaaacaatt
     9301 tcc
//
LOCUS       DACECZ010000099         8979 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.099, whole genome shotgun sequence.
ACCESSION   DACECZ010000099 DACECZ010000000
VERSION     DACECZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.099"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            13..86
                     /locus_tag="IWO35_002164"
     tRNA            13..86
                     /locus_tag="IWO35_002164"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47..49,aa:Ile,seq:cat)
     gene            complement(471..968)
                     /locus_tag="IWO35_002165"
     CDS             complement(471..968)
                     /locus_tag="IWO35_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0317213.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLFPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1008..1817)
                     /locus_tag="IWO35_002166"
     CDS             complement(1008..1817)
                     /locus_tag="IWO35_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0317214.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2151..3008
                     /locus_tag="IWO35_002167"
     CDS             2151..3008
                     /locus_tag="IWO35_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0317215.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            3001..3903
                     /locus_tag="IWO35_002168"
     CDS             3001..3903
                     /locus_tag="IWO35_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0317216.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3936..4286
                     /locus_tag="IWO35_002169"
     CDS             3936..4286
                     /locus_tag="IWO35_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0317217.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4320..5258
                     /locus_tag="IWO35_002170"
     CDS             4320..5258
                     /locus_tag="IWO35_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0317218.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5336..6229
                     /locus_tag="IWO35_002171"
     CDS             5336..6229
                     /locus_tag="IWO35_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0317219.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6267..6830
                     /gene="amaP"
                     /locus_tag="IWO35_002172"
     CDS             6267..6830
                     /gene="amaP"
                     /locus_tag="IWO35_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0317220.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6844..7032
                     /locus_tag="IWO35_002173"
     CDS             6844..7032
                     /locus_tag="IWO35_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0317221.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            7045..7947
                     /locus_tag="IWO35_002174"
     CDS             7045..7947
                     /locus_tag="IWO35_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0317222.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7979..8221
                     /locus_tag="IWO35_002175"
     CDS             7979..8221
                     /locus_tag="IWO35_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0317223.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8477..8848)
                     /locus_tag="IWO35_002176"
     CDS             complement(8477..8848)
                     /locus_tag="IWO35_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317224.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3122 a         1553 c         1740 g         2564 t
ORIGIN      
        1 tttatttatg atgtaccttt agctcagttg gttagagcag acggctcata accgtccggt
       61 cgtaggttcg agtcctacaa ggtacataag aggttgtgaa aaagctgccc tgcatcaagt
      121 aataagaaca gccaagtaaa aacagcttct tatgttttta cttggctgtt cttattactg
      181 cagatgcaag cttttgaata ccgtttaagg aagaggttgt gaaatcagcg ttttgacctg
      241 agtattagct taacaaacgg aaaaatagtt cctcatattt tgccgtttgt taagctaata
      301 ccgagggtca gctgattgaa caccatttaa gaaagaggtc gtgagcctgc cgtttcactc
      361 tgtgtcataa aaaagccaat ctactttgat agaaaaagca cgtaggacta aaacagttct
      421 gcgtgctttt tttattgtgt gttaaatatc gtaaatgaag atagatattt ttatgattcg
      481 acgatagaga cgttgaaatc tttcaacagc tctaaagcgg attctccgtg ttttctactg
      541 gagactaaca ttggatcaac aaaaaagtct gcttcaggaa aagcactctg caggcctatc
      601 agattagaga gtacacacaa gtttgtttca atcccgacta cttcgatctc tttttgttct
      661 tctttgcttt caaataaagt ttcctggatt tcagctaaag tttcaggtgg aatcgtaaag
      721 taatactttt taatcactcg ttcattttct tgtggaaaaa gaagagggat caattgcaaa
      781 tcttctcgtt cttcttcttt gtccttcagt tcaactggta tatcccgtgt gaagagaata
      841 tattcattat tttggcgtgc tttagctaat ctttgagcaa tacgttctgc caattgatct
      901 gactctggaa tataaaattc gctgtttgga tcaagaatat gattttgcat atcaataata
      961 actaacattt tctttcctcc tacattcata tcgtcatttt attcttttta atagaagttt
     1021 tttaaaatat aagcaacacc agcttcttca ttcgtcagtg ttacttggtt tgcaatttct
     1081 tttacttgtc gctgtgcatt ccccatcgca atactcaacc cgctgtaatt caacatatca
     1141 atgtcgtttt cttgatcgcc aaaagccatg atgtcactcg attcgattcc aaggtaatcc
     1201 gccagaaaaa caagtgaatg agctttagtg atacctgtag gcgtgatttc taagaaatgc
     1261 ggatcagaga atactgattg gataccatct gcttgcagat ctctttgtag cttctgtagt
     1321 ttttcttcac tcttatctgt taatgctaat ttaagatagc taagagaagg atcttccaac
     1381 atatttaaaa tttgatcaaa agaatggttc tgtaaattga aatgttcata ataagggtca
     1441 tctttgacta gatttgattc atcgaatgtc agctgatcat cttgggtttc tcggctagca
     1501 atgatggata ttttcgcttc ttgagcatag aagaaggctt ttctcagtgt atcgctatct
     1561 atttttgaac gtctgagaac tttcgccatt tttccatcct tcatttcagc aatcagtgaa
     1621 ccattggaag aaatcaaata gccaccgatt cctaattcgg aaaaaatatc tttagcagaa
     1681 atgctgttcc ttccggtagc gacggttacg attccgccat tttcatcaaa tttccgaagt
     1741 gcttggatat tttcttctgg tatctttcca tttcgatcaa gcaaggtacc gtctaaatca
     1801 atcgctgcta gtttcatttt atcttcctac tttcttttta catatctatt ttcctgttac
     1861 cttaagatta aatgagaatt taaaaaaatg aaactaactt gctttgtcat tatgaatttt
     1921 ttcaaaaaac attcttaaga aaaaggtggc tatatatagt agtttttggt caataccttt
     1981 gaataaaaag ccatgaaaaa cgaatagtta gcgcttaccg atgcgttttg tgataaaaaa
     2041 attttaaaat attcaatgaa aacaaagcat atattttgct ttaaaaccaa atgaggaata
     2101 ttctagatgt agaacaggtt agaaaaagat tagaaaaagg agtgattttg atggagaaga
     2161 gatacggcgt agttcctgtg gtaactgctt cagacgaaaa ttatgctcct tacttgagcg
     2221 tgatgatcgc aactgcatta gaaaattgca ataaaacaag acgcataaaa ttttatgtga
     2281 ttgacgatgg tctatcagaa tatagtaaag aaggattgga agaaacggta aataaatatt
     2341 caagtaatgc aagtatccaa ttcctgacag tggaaaaaga tatctacgaa gacttcctag
     2401 tcagtgacca tatcacaact accgcttact taagaatttc tttaccaaac ttattagcta
     2461 aagaggatta taagaaagtc ctttacttag attcagatgt gttagtacta gacgatatcg
     2521 taaaactata tgatgagccg ttaaatggaa aaacaatcgg cgctattatc gatccaggcc
     2581 aagtgaaagc tttggaaaga ttaggaatcg attccgatga cttgtacttc aattcaggag
     2641 tgatggtgat tgacattgat caatggaata aaaaagaaat tacagaaaaa actatccatt
     2701 acctcagtga aaatggcgat cgaatcatct atcacgacca agatgctttg aatgctgtac
     2761 tttatgaaga ttgggaacaa cttcatccaa aatggaacat gcagacttcg ctgatttttg
     2821 aacgtcatcc agcaccaaat gaaaaatatg aacgtcagta taaagaagga aatgaaaaac
     2881 cttcgatcgt tcactttaca ggacatgata aaccttggaa tacgttgaaa gatcatccgt
     2941 ataccaattt atacttaaaa aaattagcac acagtactct aacgaaagta ggcgagatca
     3001 atgaataaaa aagaaatagc agtagtcgca agttgtaaca caaaattcgt accacattta
     3061 gctgcattat ttgtctcagt attagataat tgcaacccgt ctaaatttgt tcggttctat
     3121 gtgattgacg atgatatcga ttttgaaagt aaacagcttt tacgcttctc agtgaaaaac
     3181 gcacgaatga actcagatgt ggaattcttg aaaatcaata aagaattctt tacaaatgtg
     3241 gtaatcagcg atcgtattcc agaaacagct tattatcgta ttgcgattcc ggaattgttc
     3301 cgcggcacag aagttgaacg tatcttatat atggattgcg acatgatcgc tttacaagat
     3361 atctctaaat tatggcgtct tgacttcggt gattcaatcg tagcagctgt tgaagacgct
     3421 gggttccatc aacgactgga aaaaatgaaa atttcagcta aatcaatgcg ttatttcaac
     3481 tcaggtttga tgctgatcaa tgtgaaaaaa tggttagatg aaaatattac gcaaaaagta
     3541 ttagatttca tcgaacataa tccagaaaaa ttacgattcc atgaccaaga tgcattaaat
     3601 gctatcttac atgaccgttg gttaccactt catccgcgtt ggaatgcaca aggctatatc
     3661 atggcaaaag caaaaaaaca tccaacagca gcaggtgaac gtgaatacga agaaactcgc
     3721 aacaatcctt acattatcca tttttcagga catgtgaaac cgtggagcaa ggattttgaa
     3781 gggccaacga agaaatatta cgaaaaatat gcagggatga cggctttccg ttgtgtagct
     3841 aaattcccta aatatccaaa atatgcaaaa gttcaaccaa aaacagatgt agttgttaaa
     3901 taatagaaga caacaactga agagggggag taggaatgga aattacaatg tctaaaaatg
     3961 cagtagagac gttaattgaa aaggttgggg aaaatacaaa aatcgcactc gcgcttatca
     4021 atgattccga tccatttcta agggacaaag gtgcttttgc aaaaggcagc ttttttcaaa
     4081 tcattccttt tgtttcggaa tttggagaat atgcgactaa aatcgagcat ccattattgg
     4141 acatctatac atctaaatta gagcaaaatt atttcggtaa acgattgaac atggacttca
     4201 acaaacagct tgacagcttt tcattagaaa atgaaatcgc agtactcgat tataatatca
     4261 aattaaagaa ctgcttcttc agctaatagt ttcgagaaag tccaaaaagg aggtatacat
     4321 tgagatacta ctttgtggat gaagaaaaga atgtaataga aacagatgaa aaaaaatata
     4381 attggctcgt acttgatagt gcaaagcccg aagagattga gcaagcaatc gaagaattcg
     4441 agcttcctga tgatattttc gtaggaaccg aatatccgga agaagtttct cggatggagc
     4501 gcctttatga gacgaaacta gagcatccat tttctttggt agtgattaat ctagatcagt
     4561 cagataatcg tatcgaaaaa aaattaacgc caatatcttt tgtactttcc gataatctct
     4621 tgattacttg tatcgataga aaaaccgaat ttgtggatcg gctgctgaca aagcatggaa
     4681 atcatttaaa ctcgtttgaa aagatcatca cgtatacatt attagatatt tacacccatt
     4741 acgtgaaaga actaagagag atgaaaaagc gaattgacgc tttggataaa gcagctcgta
     4801 aaacaacgga aaatgaagaa ctgtataaac aggcagatct tgaaagagac atcgtatata
     4861 tcgatcatac cttaagagat caacgggaaa caatggatca attatggaat tctgaaactt
     4921 tcatcaaacg tttagacgat gaacagctac tctatgatgt ccatttgaga cagcgacaaa
     4981 cagaaaaaat gatcgagatc tatcgagatt tattagagag tattggtgat ctattcaacg
     5041 ggatgatgga caataatcta aatcatatca tgaaatatct agattcagct gcattaatca
     5101 tttccgttcc ggcgttgttc gcagggattt ggggaatgaa tacaggagga ttaccgggta
     5161 aaagttctgc tctaggcttt atactcgtag tctttggctc tttgatagca gcagtagttt
     5221 taggttacca tttataccgg aaagattata caaattaaag aatctattta aaacaggtat
     5281 caaacaaaga aatttgtttt ggcaatacaa tttaaaaact aggaggaata cagcaatgac
     5341 agaaccaaaa ttaatcgacc cgcgaaactt gtaccatacg gagaaatttc ctaaacaaga
     5401 tcaggataca ccagctttac agcagaatat gattcctgtt cctgattgtg gagaagaatc
     5461 ctatgaaggc aataatcaac tagaaaaccg ccgtgtacta atcactggtg gagattcagg
     5521 gatcggtcgt gcagcagcga ttgcatttgc tagagaaggg gcagatatcg ctcttcaatt
     5581 cttcccagga gaagaaaaag atgcagagga agtagcaaaa tacgtgaaag ttgcaggacg
     5641 taagattgtc ttactacctt ttgatcttcg tgacgaacaa gcacctaaag aaatcataga
     5701 taaagccgtg agtgaacttg gcgggctaga tacgatggta ctaaacgcag gacagcaaat
     5761 ttcttgtcag tctattgctg agttaccact tgagcaagtc aaagacacat ttatgataaa
     5821 catcatcgct atgtttgctt tagtgaaaga agctgttccg catatcccag caggaggagc
     5881 aatcgtaaca acaacttctg ttcaagcatt caatccaagc gaacatttat tagattacgc
     5941 agcaacaaaa gcttcaatcg ctaactttac gatcggttta gcaaaacagc ttgcacctaa
     6001 aggaatccgt gtaaatggtg tagcacctgg tccaatctgg acaccattac aactagacga
     6061 aggacaacct tctgattcga tccctgaatt tggtcagcaa agtttactag aacgtgcagg
     6121 tcagccagga gaattagcac cagtttatgt tttcttagca tcgaataaag caagttatgt
     6181 tacagcccaa atttacggag taactggtgg agaagcgatc aacttataag aagttgatcg
     6241 tttcacccct atgaggaggt gcgaaaatga ataaaggtgt aaaaacgatc ggagtgatcg
     6301 gaacattact tttgctatct gtgcttttgt ttgcgatact attaagtgca acctatccat
     6361 tgccttttca gctagagaag tttcgattct tttccatcac taattcatac gtgcaacaat
     6421 atgtattttg gcttgccgtt gcatttgcta ttttgacaat tatcacgtta cttgttttcc
     6481 tgttttatcc aaaaacaatc ggaaactttc tactgaaaca tggtgatggg gagttgacgt
     6541 tggataaaaa agcaattgaa ggaatggttc gttctcactt gcatgaagat gaattcatcc
     6601 attcaccaaa agtaaatgtc aaagcaacga aaaacaagat cgccgtcaat gtaaaagggg
     6661 agttgaaacg aacttcagca ctcattggta aaacaggaac attgatgaaa gaaatcgaaa
     6721 atgaagtaca acacatattg ggaacaaaag agccagtgaa agtatcggtg aaatacacag
     6781 gctttgaaga accagctaaa cgaagcaatg accgcaaacg agtagaatag ggggggacca
     6841 aaaatgaaag aactattttc gacatacaag ctccctatca tctttggggt acttggattg
     6901 atattagccg ttttatggat caccgtgggc tttttcaaga ccttattgat cttgatcatg
     6961 gcagcgatcg gcgtcgctat cggtttttat ctgaaacaaa cgaaaatctt agatcactat
     7021 tttaaatctt aggaggaaac atccatgaat aacaataaca aaaataataa caataaaatg
     7081 aacaaccaaa aaccagtgtc tgcacatcca gacccaacga caccaaacca atcaaatggt
     7141 gtgaacgatg aaaagaacaa aacagcacca gtatctgcac atccagatcc agtagtccct
     7201 aacgttcaac aaggagtaga agagaagaaa catgatgcag aaaagcatgc acctcatcat
     7261 ccaggaactc cagaatctaa agttccagaa caacatgatg caatcgatcc ttctaaacct
     7321 gtggaagagc aaaaacatcc ttctgaccat gcgaaagcac aagaaaaact tagttctgac
     7381 cacacaaacg gacaaggaaa acttggttct gatcatacaa ataaccaagg aaagcatgat
     7441 tctgaacagg aagattcaat gcgcggtgag ctgaattatg aagataaagt tgtgcaaaaa
     7501 atcatcggaa tcgcaattga acaagtagac ggtttattat ctgcaaacgg tggtttcttc
     7561 tctaatgtgg caggaaaact tgtgaataca gataatgtaa cggctgggat cgaaacagaa
     7621 gtcggtaaaa aacaagtagc agttgatatg gatgtcattg tggaatatgg caaagatatc
     7681 gaaaaaatct ttgaagaaat gcaagaagta atcggcaaag aagtaaaaaa tatgacacat
     7741 ttagaagtga tcgaagtcaa cgctaacgta gtggacatca aaacaaaaga agaatttgaa
     7801 aaagatcaag aaacggtgca agacaaagta actgacgcag cgaaaaaaac cggtgaattt
     7861 gcttcgaatc aaacagataa agtaaaatca gcagctggaa gcggcgcaca aaaagtaaaa
     7921 gaaaatacag aaccacgggt tcagtaataa ggaagtaaaa cattaggagg aacgaaacat
     7981 gggatttatt tggtcactta tcgtcggagg aattatcgga gcaattgcag gagcaatcac
     8041 gaataaaggt tcttcgatgg ggattattgc aaatgtcgta gcaggtttag ttggatcagc
     8101 acttggtcaa gcattattag gttcttgggg accaagtatt gcaggaatgg caatcgtgcc
     8161 atcattgatt ggtgccatca ttttagtact aatcgtatca ttctttgctc gaaaactgta
     8221 aaaagacttt taaaaagagg tcgtgaaaaa gctgttcttc atcaagtaat aagaacagcc
     8281 aaacaaaaat agcttctcat atttttcatt tggttgggca tattatcgaa gatgcaagct
     8341 tttgaacact gtttatacgg aataagaggc tgtgacaaga cttttgtcac agcctcttat
     8401 ttatgcgggc aaattcccaa ttagattttt aaaagttttt ccttaaatgt gtctttattg
     8461 atacagagaa gcttctttaa taaaacaatg cctccctaaa ttttcttgag ataggaaagg
     8521 tgaacataca aaataatacg accactttga aaacccagat gatccacaaa tggatcgtat
     8581 gatactgatg aagtaaaaag caatggatca gaaactcaaa gagattgaaa aagacaagga
     8641 agaaataata tctctttttt actcttgtct gttttagcca ataatagctc gtaaagtaat
     8701 ctagacaaac aaatagaagt aaacaaagtt tcgtaagagc agcaatcgta tcatgttgct
     8761 ctgctagacg attgacgagc aggaaaaatg ttggtaatgg aatatagatc acgagtaatg
     8821 aaagaaaaaa gtaaacaata aatggcatac tattcctcct aattgtgtga gttcgccctc
     8881 attttaaaat aacgatattt gtttgtgaag ctttttcgtg tcgtaacaaa tgttatctaa
     8941 tgtattttta aataaataat cattattatc aaaataatg
//
LOCUS       DACECZ010000100         8924 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.100, whole genome shotgun sequence.
ACCESSION   DACECZ010000100 DACECZ010000000
VERSION     DACECZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.100"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            17..1204
                     /locus_tag="IWO35_002177"
     CDS             17..1204
                     /locus_tag="IWO35_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HAQ0317225.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            1185..2489
                     /locus_tag="IWO35_002178"
     CDS             1185..2489
                     /locus_tag="IWO35_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ0317226.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            2515..3528
                     /gene="citC"
                     /locus_tag="IWO35_002179"
     CDS             2515..3528
                     /gene="citC"
                     /locus_tag="IWO35_002179"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAQ0317227.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            3532..3825
                     /gene="citD"
                     /locus_tag="IWO35_002180"
     CDS             3532..3825
                     /gene="citD"
                     /locus_tag="IWO35_002180"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAQ0317228.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            3830..4735
                     /gene="citE"
                     /locus_tag="IWO35_002181"
     CDS             3830..4735
                     /gene="citE"
                     /locus_tag="IWO35_002181"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAQ0317229.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            4728..6287
                     /gene="citF"
                     /locus_tag="IWO35_002182"
     CDS             4728..6287
                     /gene="citF"
                     /locus_tag="IWO35_002182"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAQ0317230.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            6284..6841
                     /gene="citX"
                     /locus_tag="IWO35_002183"
     CDS             6284..6841
                     /gene="citX"
                     /locus_tag="IWO35_002183"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAQ0317231.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            6834..7697
                     /gene="citG"
                     /locus_tag="IWO35_002184"
     CDS             6834..7697
                     /gene="citG"
                     /locus_tag="IWO35_002184"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAQ0317232.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            7931..8340
                     /gene="tnpA"
                     /locus_tag="IWO35_002185"
                     /pseudo
     CDS             7931..8340
                     /gene="tnpA"
                     /locus_tag="IWO35_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         3115 a         1386 c         1819 g         2604 t
ORIGIN      
        1 aaaaaggagt gaaggtatgc ttgatgaagt tttagaaatc cataaaaaaa atgtaggtgt
       61 tttacggatg gaaacaacag aagccgtgca agatgatcaa gatttagcaa aaatgtacac
      121 cccaggtgta gcagaactaa gtttaatgat tgagaaaaat catgctttgg caagagaatt
      181 aacaattagt ggtaaattag tggccgttat tacagacggg tcggcggtat taggtcttgg
      241 aaatgtagga aaccaagctg gattaccaat cgtcgaaggg aaagcgctgc tttataaaaa
      301 tttagcaggt gtcaatgcga ttccattagc catcgaacaa aaatcagtag acgagattgt
      361 acaaacaatt gtcaatttgc aaaatagttt tgcagggatc catttagaag atattgcagc
      421 accgaagtgt tttgagattg aagaaaaatt acaagaaaaa ctttctattc ctgtgtatca
      481 tgacgatcaa gaaggaacag cgatagttgt tttagctgga ttgatcaatg ctgctaagat
      541 ccaaaggaaa acattaactg aacttcgagt tttgatcaac ggcatgggag catctggggt
      601 tgcaacagct agactattaa tcgctgctgg tatcaaaaat ctaacattgg tggataaaca
      661 agggagatta aagaagcaag atagtacatt aaataaatat caaagagagt tgttggatca
      721 tatacatgat acaaataata atgcaagcgt tttggaagat gcaatcgttg gacaagatgt
      781 ttttatcggt ctttcaatag gaaatgtttt aacgcctgaa atgattggaa aaatgaatga
      841 taatccgatt atttttgctt tagccaatcc agtaccagag atcacacccg atttagcaaa
      901 agaatctggt gcaaagctaa tcgctacagg ttcttcgaaa tatcctaacc aagtaaacaa
      961 tattttagta tttcccggat tgttcaaggg actattacta tcgaaagcga ataaagtcga
     1021 tctttcatta caacttgaag tagccaaaca attagctgct aaggtcgcag agcctacggt
     1081 agaaaaattc atccctaatg tttttgatga aggagtcgca aacgtcgttt cacaggcagt
     1141 tttggattat ttacaaaaac taaaagaaaa cgaggataat atacatgctg aaaacattaa
     1201 atagaattaa aattggtttt attcctttat ctctttatat cattttggta ctcgcgtatc
     1261 ttgcaatgac gataagcaat atcgttccag aaaatgatat gttagtttct ttgggtatta
     1321 tgactgtttt tgcttattta ttagaagaaa ttggtaaaac tattcctatt ttaaaagatt
     1381 taggtggtaa agtattagtt gttacatttt tgcctgcatt tctagtctat aaacattggc
     1441 tacctcaaag tactgtaact gtggtttctg attttatgaa taataacaat ttcttgatgt
     1501 tctttatcac actactagtt gtcggcagta ttgcttcaat gaatcggcaa acattggtta
     1561 aagcaacttc acgtattatc attattttaa ttttatgcga tgtgattggt tctttgatag
     1621 gaacaggtgt gggaatactc cttgggctat ctgcttggaa tgcgtatttc tttatcgttg
     1681 cgccgattat ggctggtggt gttggggaag gagcgatgcc attatcgatt ggttatgcag
     1741 caatattatc tggtatggga caaaatgata tctttgggaa gattctaccg tgtgtgatgt
     1801 tgggaagttt agtcgcagtt ctactgtctg gtatgctcaa aaaaattgga gagaaatacc
     1861 cacaattaaa tggtcagggt aatttgatcg atggggatga tgaacattta gagaacttga
     1921 agggttcagc taaaccagtc aatattgaaa aaacattgtt agcaggcgtg gtagcaataa
     1981 cgatttattt tgttgctgtc tatgttgagc acttattaaa atcactcctt ggcttttcta
     2041 ttccttcacc tgtgatctta ttagtctctc ttatgggggc taaaatgctt ggttttatcc
     2101 ctaaaaatat cgaagaaggt ggaaattcgt tgtatggatt cactgttcgt gggatcacac
     2161 ctcccttact ttttggcgta ggggttgcaa aaacagattg gttagagctg atcgaagtat
     2221 ttaaaaatcc tgcaacgata gtaacaatca ttgtgacggt agtagctatt gtcgctacag
     2281 cttttatcgc cgcaaaattt ttaaaaatgt acccagttga tactgccatc gcagtttcat
     2341 gttgtagcgg acaaggcggt acaggtgctt tagctatctt agctgcgggt gatcgcatgg
     2401 aattaatgcc atttgcacaa gttgctgttc gactaggggg tgctatgacc gtaacgttcg
     2461 ctatcttttt aatgggtctg ctatcataag aacataaaga aaggacgtga ccatgtgttt
     2521 gatcaaacag atatccaacg attattttta aaaaataact atatcaaaga ccaatgggaa
     2581 aatcttttgc ttgattcagg aatacaaaag aagcaaatcg aagattttca acatttagat
     2641 caaacattgg gtatatacca taaagaaaag ttagtaggaa cagtatctta tcaaaacaac
     2701 gtgattaaat atatcgccgt gtctgaaaaa tataaagatg gtggacatac atttaatgta
     2761 ctagtctctg caataattca ggcaatgagt caacaagaga tttttcatta tattgttttc
     2821 acgaaaccgc gctatcaaaa aagttttaac tttttaggtt ttgaaacaat cgtatctacc
     2881 ccaaaaatag ctgtattaga aatgggagat agttctataa aacagtacct agagaggatt
     2941 cctaaagtga atggtgaaaa aaaagcagca attgtcatga atgcgaaccc atttacagaa
     3001 gggcattatt atttagtaaa acaagctgcc aataacaacg attgggtata tgtatttgta
     3061 ctcgaaaaag aacaaaatct tttttcgacg aaggaccgac tcaaattagt tcaacaagga
     3121 ttacaaaaac tgaaaaatgt cattgttcta tcaggtggcg agtatattat tagccctgca
     3181 acgtttccca cctatttttt acgagaagat gatgaaatag caaaggagca aatggtcgta
     3241 gatgctactt tattcaaaga aagaattgcc acttatttag atatctctgc tagatatgtg
     3301 ggagaagaac cactttcccc aatgactcgt tcatataacg acatactgga aacgatttta
     3361 ccccctgaaa taaaagttca tgtccttgca aggaagaaaa cggaacaaca ccaagttatt
     3421 tctgcttctc aagttcgtaa agcgtatctt gctgggcaat tggaaaaaat aaaatatatg
     3481 gttccagaaa caacctatca atacttaaaa aataaaaggg agcgttaagt aatggaaata
     3541 aataaatcag cgattgctgg aacattagag tcaagtgatg tccaaatcat gatttcaccg
     3601 aatacagaag gaatagaaat cgatttagac agtcaagtga ttgagcaatt tggaaaacaa
     3661 atcaaaaaag ttatctatga cacactagca gtttatcaga ttaaaaacgt aaaactcaag
     3721 gtagtagata aaggggcatt agattgcgtt attgttgcac gattaaaaac agcgatccat
     3781 cgttccttag gtatccaaca ccaagaaaca ccgtgggagg tttaattaga tgatgaaaga
     3841 acgtttgaga agaacaatga tgtttgttcc aggaaataat ccagcgatga tcaaagatgc
     3901 aggtatttat ggtgctgact cgattatgtt cgacctggaa gatgcagtgt ctatggcaga
     3961 aaaagacagc gcaagaatgc ttgtggctga ggcattgaaa acagtggatt atggtgacac
     4021 agaattaatt gttcgaataa atgacctgca ctcagaattt ggacgtgcag acatttttgc
     4081 aatggttaaa gccggcgcgc atgtattacg tttacctaaa acagaaacct ctgaagatat
     4141 aacagaagta gaaaaagtga ttatagaagc agaaaactat cataatgttc cgatagggcg
     4201 aacattgatg atggcagcca ttgaaggtgc ttctggtgta ttgaacgcac ctgcgatcgc
     4261 caaagcatct gatcgattga ttggaatcgc cattggggca gaagactatg tgacaaatat
     4321 gaaaaccaaa cgatatccag atggaaacga attgtttttt gctcgcagta tgatcgttca
     4381 tgctgcacgt gcagcaggca tttccgcatt tgacactgtt tactctgatg tggatgatat
     4441 ggaaggattt ttaaacgaag tacgtttaat ccatcaatta ggttttgatg gaaaatcttg
     4501 tatcaatcca cggcaaattc cagtagtcaa tactgtttat cagccgtcaa tcaaagaaat
     4561 caaacgatca ctagacatca tctcggcaat cgaagaagca aaagaaaaag gttctggcgt
     4621 aatttcttta aatggaaaaa tgatcgataa accaattgta gaacgtgcac aacggacatt
     4681 gatgttagca aaagcatcac gattgattga tgaggaggga aatatccatg cataataaac
     4741 aaagacgtag cattccatca agtgttttaa caaagtataa aaaagaattc caaagtgttt
     4801 cattatcgcc acaaacgatt caacgttgtg caccaaaagt aacgactgta actaatcaga
     4861 caaataaatt gatgcactcg atcaaagaag ctatagaaaa atcgggctta aaagatggaa
     4921 tgacgatttc atttcatcat catttgagag aaggtgattt tgtttttaac caagtgatgg
     4981 ccgtgatccg tgaaatgggc atcaaagaat tgaccttagc accaagttct ttaacgaaca
     5041 ctatgaatga gatggttatt gaatgcatca aggacggtac agtaactcat atcacaagca
     5101 gtgggatgcg cggcagctta ggtgaattta tttctcatgg aggaatggac aatcctgtga
     5161 ttttacgttc tcatggtaat cgtccacgtg caattgaaag tggtgaattg aaaatcgatg
     5221 ttgcatttct aggagtacca acatctgatg attatggaaa tgctaatgcg actgttggaa
     5281 aatccgtttt tggttcatta gggtatgcaa aagtagatgc acaatatgca gataaagtca
     5341 ttcttctaac tgatcattta gttccttacc ctaatacgcc cattagtatt ccgcaaacac
     5401 aagtcgacta tgtcgtggaa gtagaagcaa ttggtgatcc gaataaaatc ggtgctggtg
     5461 ctacacggtt tactaaaaac ccaaaagaat taaaaattgc tgaaatggtc aaagatgtca
     5521 ttgttcactc accttatttc aaagatggct tttcttttca aacaggaacg ggtggtgcag
     5581 ctttagcagt aacgcgttac ttagctagtg aaatggaaga aaaacagatc aaagcttctt
     5641 ttgcattagg tggcatcact aaaccaacgg ttgatttatt ggaaaaaggt cttgttgaaa
     5701 aagtgttaga tgtccaagac tttgacaaag gagcagctga aggaatggcg cggcttgaga
     5761 accaacagga gatcgatgct tccttttatg ctgatccaag taataaagga gcggttgtta
     5821 accaattaga tgtcgctatt ttatctgcat tagaaattga cacaaacttc aatgtaaatg
     5881 ttatgactgg ttcagatggt gtgctaagag gtgcgattgg tggacatcaa gatgcagcaa
     5941 cggctaaact tactatcatc tcagcgccgt tagtacgtgg ccgaatacct acagtagtaa
     6001 atgatgttac tacattgatc acaccaggga aaagtatcga tgtattagtc acagaagtag
     6061 gggttgctat caatccccaa cgtaaagatc taatagctat ttttgaaaga attccacaaa
     6121 ttcctgtttt tacaatagaa gaattacagc aaaaagcaga aaaaatagta ggcatcccgg
     6181 aacctttgca attcacagat cggatagttg cctatgtaga ataccgagat ggttcaattt
     6241 tagatgtcgt tcgtcaggtg aaagaatttg aagaagagag atcatgatgt ttgatactgg
     6301 agaaattcaa gaaatcgagg atattttgat ccataaagaa caacgtgcta acttgcaaag
     6361 caacctgctt tccaagtatt caaatagttc attgatggtc tttacattga atattcctgg
     6421 gccgatcaaa aataatccaa tgattgagca ggtatttcgt cagggacagc agtcaattca
     6481 gcaaatgttt ttaaaaaaaa aggtggaaat gacgtacaca aaagaaatca atttgccaag
     6541 tggattggac tggtacgtaa tagtaacaga agcacctttg aatctcaaaa aatggttggt
     6601 cacgttagaa gagcaagacg cattaggtag actttttgat ttagatgtct tgttcaaaaa
     6661 aggagagcag ataacaattg tcagtcgcca agctcttaat catggggaac gtccttgttt
     6721 tatttgtggg aaatcagcaa aggtatgtgc acgtaatcgt acacatactg tgaaagaaat
     6781 gcaaaagaaa atcgaacaaa tagtagccga ttccttaaaa ggagcattga aacatgcatg
     6841 ataatattca aaaaatagca caagaagcac aattagcgat gctttatgaa gttacatgta
     6901 tcaataaacc aggattggtt gatccagtag accccgggtc tcatcaagat atggacattt
     6961 ttacttttct acaaagtagt gtagttttga ctccctattt tgaagaattt atccaaacag
     7021 gattggaccg acaacatcaa ccaattgaag aaacctttat ggcgattcga cagattggtt
     7081 tagaggctga aactgcgatg ttctcagtga caaatggtat aaatacacat aaaggtgcta
     7141 tcttttcatt ggggattttt cttgctattt gtggccggtt aactatttgg aaagttcctt
     7201 gtaaaaaaag tttctttcaa aaagaaattc aaaaaatgac gaaaaatcta cttaacgatt
     7261 ttgggaagat tgatcaaaca aaaccaaaag catggacttg gggagaatat ctatttgtta
     7321 accatggatt gacagggata cgaggggaag ccaaaagagg ttatccttgt gtttttgacc
     7381 gaggattgcc ttattatcaa gcatatcaag ggtcgcaaca agataagatg atagacactt
     7441 tactttttct atcaatggaa gtagaagata ccaatttgat caaacgaagt aataatagac
     7501 atgtttttga agagtatcaa ttacttattc gtccttattt tgaattaggg ggtgtaaaaa
     7561 ccatacaagg taaaaaatat ttggattacc taaatcagca atttaaaaag aaaaattgga
     7621 gtattggtgg aagtgcagac ttattgatcc ttacaatctt tttagataaa atcatggaca
     7681 aaggatggtt atgttgatac tataatacat tattttgtat tagtaagcta ataactaaaa
     7741 aatggttcaa tacaaatatt ttataaaaaa tggtagtgcc tatcctttca gattgtagac
     7801 aaagaaaaat ttctttgtct acaatttttt tgttaatgac ttcagtcagt ttcgtgcgca
     7861 aagcgtgcga aacaagaaaa ccatgcgttt ctacacgtgg aagaactagc atatcgaaag
     7921 gagttttttc atgtcaaatg acgataaaag tttagcccat actcgttgga attttaagta
     7981 tcatattgta tttacaccaa aatatcgacg aaaagtaatc tataggcaat tacgaaaaga
     8041 tattggaaaa atacttagga agttgtgcaa aataaaaaac gtggaaatta tagaagcaca
     8101 tgcaacgcca gatcatattc acatgcttgt tcaagtacca tcaaaaataa gtatatccag
     8161 ttttatagtt atctgaaaga tcgtagtgct gtgctaatcc acgagcagca cgtaaatcac
     8221 aaatatagat acggaaataa atcattttgg tcggaggaat attatgtgag tacagtcgaa
     8281 ttgaatcaaa agacaattga aaaatacatc cgtgaacaag aagctgatga tcgcatttga
     8341 gatagtataa gtaaaagaga gtagatgttg caataacaaa agggtaaaaa tggactacct
     8401 ccagtaaaat accgaaaaat agtttaactt ttggagataa gtccatttaa gggtctatag
     8461 aatgacaatt tttttagggg attttactat tattacttca agacccgctt ggattcgcaa
     8521 agtgagtgaa ctcttaaaac tacctgatag taggttgttt taagagttat ctgaaagtaa
     8581 aaattgcatt tatatttttg ataaatacgt aaatttatat atacgtattt atcaaagttt
     8641 taggccaaag gaatattgtg taaatacact aggattgaat gaatagacca gaagaaattg
     8701 cgtccaagaa caaaagaagc atgatagtgc tctatataaa ttgggcattc aagaatctga
     8761 aatttaaagg tagaatttta gttagacttt tagattttaa acacaaaaat aaccatacaa
     8821 gcaacaagaa gcaaagtgct atattataaa ttgtccgtcc gtttacttac ataagatcat
     8881 tggggctatt tttaaaagag aataatagat aataaatgaa aaaa
//
LOCUS       DACECZ010000101         8878 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.101, whole genome shotgun sequence.
ACCESSION   DACECZ010000101 DACECZ010000000
VERSION     DACECZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.101"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            537..1002
                     /locus_tag="IWO35_002186"
                     /pseudo
     CDS             537..1002
                     /locus_tag="IWO35_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            1345..1601
                     /locus_tag="IWO35_002187"
                     /pseudo
     CDS             1345..1601
                     /locus_tag="IWO35_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            1988..2215
                     /locus_tag="IWO35_002188"
     CDS             1988..2215
                     /locus_tag="IWO35_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ0317233.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            2231..3397
                     /locus_tag="IWO35_002189"
     CDS             2231..3397
                     /locus_tag="IWO35_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ0317234.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            3632..4675
                     /gene="hrcA"
                     /locus_tag="IWO35_002190"
     CDS             3632..4675
                     /gene="hrcA"
                     /locus_tag="IWO35_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ0317235.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            4769..5332
                     /gene="grpE"
                     /locus_tag="IWO35_002191"
     CDS             4769..5332
                     /gene="grpE"
                     /locus_tag="IWO35_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ0317236.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            5383..7212
                     /gene="dnaK"
                     /locus_tag="IWO35_002192"
     CDS             5383..7212
                     /gene="dnaK"
                     /locus_tag="IWO35_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ0317237.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            7363..8529
                     /gene="dnaJ"
                     /locus_tag="IWO35_002193"
     CDS             7363..8529
                     /gene="dnaJ"
                     /locus_tag="IWO35_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ0317238.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         3102 a         1447 c         1923 g         2406 t
ORIGIN      
        1 agaaagaatt actaaaaaaa ttagatattt ctactggtgt attagctaat ttaattagaa
       61 atggcatgcc aaaagaggga gaaatgttta atcttgataa gattattaca tggcgagaaa
      121 attggtctaa aaacatatta ggtgagttag aagttggtag agtatatact aacaaagaaa
      181 tttcagagaa gtttaaatgt tccaaacagg gtggaatgag aagatctcat caaactaata
      241 ctcttgtact gtttagtgat caaacaggaa gtaatgttta taaagataag tggttaaatg
      301 gtattttaca atataccggt atggggctaa aaggggatca agtattggat aagaatcaaa
      361 ataaagtttt agccaattca aaaagtaatt ttgtaaagat tcatttattt gaaacattta
      421 aaccaaaaga acacacatat ctgggagaag tatatttagc aggacaaata tatactgtta
      481 atgaaaaaga tagttcgggc aacagcagaa aagtatataa atttccatta gctttaataa
      541 atcaagagca attgattgaa gataaagata tataaccagg aagaaaatca aactagacat
      601 attcgtaatt tatctaatgc taaattagag gaagaagcaa gaaaagttag taattataat
      661 aaaaaaatgg ctgcaaaata tgttaacgaa gaggagtttt gcttctcaaa aacaaaaata
      721 tataaacgta atcccttcgt atctgaatat gttaaacgac ttgcaaaagg tatttgtcaa
      781 ctttgccaag aaaaagggcc atttataaaa gacggtgtac cttatttaca ttgtcatcat
      841 atagaatatt tgtcgcaagg tggaaaagat gtgattgaaa actgtatcgc gttatgtccc
      901 aattgtcatg ctagaattca tgaacttgag ttacctgacg ataaagaaaa attgctgaag
      961 atcgttgaaa aaagagaaaa tagcttttct aaaataccgt aatttatttt tataggacaa
     1021 ataagctaat gtgagctatg ccttctatat aagactaagt tctattataa gtaacgaagt
     1081 aattctcgtt ccttcctgta gtaatatttt ttaaataacc tataggcttc ttcaaaagta
     1141 catcctccag taaattctaa gtactggagg attctttttc tagtgttgct ttactcatga
     1201 aaaagtagtt ggctacttca tcaaaatttc tgaacttttt taatgtataa taaagagctc
     1261 gatcatttac tacaaggatg ctagctccta cattttcaca gttttttagc aagtaaattt
     1321 ttctaaaatc acatcaggaa atgcatgaga ttctttaaga gaggcaatta tagggctatg
     1381 attacggttc gtgtaagtga cgccaaaaaa gaatggcttt cttatatggc tgaattttat
     1441 gggattactt tatctgaact tctaaaaaac tattcgatgg aacaactaga agatgaatac
     1501 aatcgtcaaa cggctgaaat tgcccataaa cattggataa gaagtgggaa aacaacagta
     1561 ttcatggaag aatccttaat gaatttggtg ggctggaatg acctgccgtt taaaattcac
     1621 gcccgaaatt cagaagcagc tctgattggc aaatacgtgt attggtagag atagaagata
     1681 atcaattcgt tgttatcgct attcaagtag ggcaccgaag aaatgtctat gattaagaga
     1741 ctgtttattt ttacaaaaaa ataataattg gaatctagtt aaaactcttg aagtgagaga
     1801 tttgactaga tttttttatg aataaaataa ggacaaaatt gtttaggaag aatcatgagt
     1861 gtaaaaaaat catccaagaa gtcgattctt ggatttttta gtggatttgt tcgccaaata
     1921 cgtttagtat gcgtatatga gaggttgtaa gcaaagcgtt gtggtctgag cacttaagga
     1981 ggaatctgtg aaaatagctt ttcatatttt tgcagatttt gacttaatgc cgaaggtcag
     2041 ctttgcgatt accgtttatc gagaggctgt aaaagcaccg tgtagctccg atgacaataa
     2101 gcaagcattt ttagagctag gtttccttga ttctaaatat gcggcttatt tcaatgagtt
     2161 ggtgcttgaa cgcgatttta agaaggtcga aaacaggcac gacttttcga cgtaaggaga
     2221 aagaatttca atgatagaaa caataaatcc cgcagaaaac aaggtttctg cgtacattca
     2281 tatcccattt tgcgagcata tctgctatta ctgtgacttt aataaagttt ttttagaagg
     2341 gcagccagta gatgagtatg tggaaatgct gctgaatgaa atgagattaa tgctggaaag
     2401 tcatcctaca gcgaatttgg aaacacttta tgtaggaggc ggaacaccaa cttctttatc
     2461 tgctaaacaa cttgatcgac tattatcagg tgctagagag atcctgcctt ttcaaaatgg
     2521 taatgagttt actgtagaag cgaatcctgg tgatttgacc cgtgaaaaac ttcaagtgat
     2581 gaaaaactat ggcgtcaatc gtttatccat gggagtgcag acatttgaca atcgcttatt
     2641 gaaaaaaatt ggacgtaaac atactgcaga agatgtgtat caaacaatgc aatttttaga
     2701 agcagaaaat tttaccaatg taagcatcga tttgatttat gcattaccgg gacaaacact
     2761 agaaggatat cgggatactt tggaaaaagc tttggctttg gatctgcctc actattcttt
     2821 gtactcattg atcctcgaaa acaaaacaat gttcatgaac tgggtacgac aaggaagact
     2881 cgaactgcct gatcaggaaa cggaaaccag aatgtttgaa gagacgattg aagcaatgga
     2941 aaagcatgga cgtcaccaat atgaaataag taactttggg ctctccggac atgaatctca
     3001 acataacctc atgtactgga acaatgacca ctattttggg tttggagctg gtgctagcgg
     3061 ttatcttgga gatattcgtt accgcaacaa aggaccaatc caacactatt tacgcccatt
     3121 acgagcaaat gaacgcccgg tattggaaga agaaaaattg acacgaaaaa atcaaataga
     3181 agaagaaatg ttcttaggac taagaaaaaa aacagggatt actaaaaaac atttctatga
     3241 tcgctatcaa aaaacaattg aatcggtgta cggtgatgtg ctaagggaat tagagcaaga
     3301 agggttactt gttaatgaag aagaccgtgt ttatttgaca ccaagaggga cttttttggg
     3361 gaacaatgtg tttgagcgct ttttatttga aaaatagttt gaagaatatt tgttttttat
     3421 cgaaacttga aaagaaaaag tttagaaagt ttgacaatga ggtattgaag tattgatata
     3481 actggattta catcaattat ttagaattag cactcttgtg tttagagtgc taaaaaatgg
     3541 agtctttctc ttgacaggag aagggcttct tgctatattt ataagtgaat tagcacttag
     3601 aatatatgag tgctaatgag aggtgaggaa gatgttaaca aagcgacaaa acgacatttt
     3661 acgtctgatc atccaacact atacgagtag tggcgtgccc gttggttcca aaacattaat
     3721 gtctgaggga gtcaaggcca gctcagcaac gattcgcaat gatatgaaag cattagaaga
     3781 tgaaggattg ctgttgaaga ctcactcttc ttctgggcgg attccatctg ctttaggcta
     3841 tcgttattac gtggatcatt tgttaaaacc tgctcgcgta gcagaagatg atttgctgct
     3901 gattcgtcag tctctaggca gagaatttca tgaaatcaat gagattatta agcaatctgc
     3961 tgagatatta tctgagctga caagctatac gactttttca ttgggacctg aaataaaaga
     4021 ccgccgactg actggctttc gtatcgtacc cttgaataat cgacagatta ttgctatcgt
     4081 cgtaacagat atggggaatg tagaaagcca agtgttcact cttcccgaat atatgggaag
     4141 tcaggaacta gaaaaaatgg tacagattgt aaacgaccgt ctagtcggag atcctctgat
     4201 gacggtgtac cataaacttc gtacggagat tccgatgatt cttcataaat atttccagac
     4261 gacagaaggg atgtctcagt tatttgacac cgttcttggt catgtttttg aagaaaaggt
     4321 ttatgtcagt ggacaaatga atctcttgga ttatgagccc cgtcaagatc ttgatcagtt
     4381 caaatcgatg ttttctttta tgaaagactc agacggactt acacaaatga tcactccgat
     4441 ggatagcaat attcatatca agattggtgc tgaattaggc aatgatttat tgcagaatat
     4501 gagtatgatt caagcaagtt acgagataga tggtcatgga agaggtacaa tagccttgtt
     4561 agggcctact agtatgcctt attctcggat gttcggtcta gtcgatgttt atcgaagaga
     4621 actagcagct aaacttgctg attactaccg ctcgcttgac ttgtcaaact cgtaatgtga
     4681 ataacgaagc tatttgcagc tgatcgttct ctctttctta gtaagagcaa tcaaacagct
     4741 gactgaataa tgaaaggaag actgtatcgt gaaagaaaat caagaagaac tagataaaga
     4801 aatgacagat gctcagccag aaccggaaat cgatgtcgaa gctgcagaag attcgggtat
     4861 ttcagaagct gaagcggaag aattcgaaac agcgaaatta aaagctgagt tagaagaaat
     4921 ggaagataaa tacttgcggg ctcgtgctga aatcgcaaat atggcaaatc gaggaaaaaa
     4981 tgagcgtgag caattacaaa aataccgttc acaagattta gccaaaaaat tacttccatc
     5041 aattgacaac ctggaacgtg cattagccac agaagtgtca gatgatcaag gggcaggttt
     5101 gaaaaaaggc gtggaaatgg tcttggaaag tttgcgtaac gcactggaag aagaaggaat
     5161 tgaaaaaatc cctgctaaag gagaagcctt tgatcctaat cttcatcaag cagtacaaac
     5221 tgttccagcg actgaagata caccagccga tacgattgta gaagtactac aagaaggcta
     5281 caaattacat gatcgcgtgt taagaccaac tatggtcatt gttgcacaat aattgtgcgg
     5341 atataaaact tacactttca attaaagaag gagacgttaa ttatgagtaa aattatcggt
     5401 attgacttag gtacgactaa ctctgcagta gcagtattgg aaggaaacga agcaaaaatc
     5461 atcgctaacc cagaaggaaa ccgtacaaca ccttctgttg tatcattcaa aaatggagaa
     5521 atccaagtag gggaagttgc aaaacgtcaa gctgtgacaa acccaaatac gatttcatca
     5581 atcaaacgcc atatcggtga agcaggttac aaagtagatg tagaaggtaa atcatataca
     5641 ccacaagaaa tctcagcaat gatccttcaa tatatcaaag gattcgcaga agactattta
     5701 ggtgaaaaag tcgaaaaagc agttatcact gtgccagctt actttaatga tgcacaacgt
     5761 caagcaacaa aagatgctgg taagatcgct ggtctagaag tagaacgtat cgtcaatgaa
     5821 ccaactgcag ctgctcttgc ctatggttta gataaaacag atcgcgacga aaaaatctta
     5881 gtattcgacc ttggtggcgg tacttttgac gtatctatcc ttgaactagg agacggtgtc
     5941 tttgatgtat tgtcaacagc tggcgacaac catctaggtg gggacgactt tgataacaaa
     6001 atcatcgact acatggtagc agaattcaaa aaagaaaaca gcattgactt gtctcaagac
     6061 aaaatggctt tacaacgctt gaaagacgct gctgaaaaag cgaaaaaaga tttatctggc
     6121 gtatcaagca cacaaatcag cttgccattt atcacagcag gagaatctgg accattgcat
     6181 ttggaaatga cattgacacg tgcaaaattt gatgaattaa cagctgactt agtagaacgt
     6241 acaaaagtac cagtacgtca agcgttaaaa gatgcaggtc tgtcacaatc agaaatcgac
     6301 gaagttattt tagtcggtgg atctactcgt atccctgcag ttgtagaagc agtacgtaaa
     6361 gaaacaggaa aagaaccaaa caaatcagtt aaccctgatg aagtagttgc tatgggtgct
     6421 gctatccaag gtggcgtgat cactggtgat gtgaaagatg ttgttttact tgacgttaca
     6481 ccattgtcat taggtatcga aacaatgggt ggagtgttca ctaaattgat tgaccgcaat
     6541 acaacgattc caacaagtaa atcacaagta ttctcaactg cggcagataa tcaaccagca
     6601 gtagacatcc atgtattaca aggtgaacgc ccaatggctg ctgacaacaa aactctagga
     6661 agattccaat tgacagatat tccagcagct cctcgtggta ttccacaaat cgaagtaaca
     6721 ttcgatattg ataaaaacgg gattgtaaat gtatctgcga aagatctagg tacacaaaaa
     6781 gaacaaaaaa tcacgatcaa atcttcttca ggtctaacag acgaagaaat cgaacgtatg
     6841 gtgaaagatg cagaagcaaa cgctgaagca gataaagctc gtaaagagga agttgaccta
     6901 cgtaatgatg tagacgcatt gctattctct gtggacaaaa cattgaaaga attagaaggc
     6961 aaagtggacg aagaagaagt gaaaaaagca gaaacagctc gtgacgaatt aaaagccgct
     7021 gttgaagcaa acaacatcga ggaaatgaaa actaaacgcg atgcattgaa cgaaatcgtt
     7081 caaaacttga ctgttaaact atacgaacaa gctgcacaac aacaagctca agaaaatcca
     7141 gaagcagctc aaggcggagc agatgatgtc gttgatgctg attttgaaga agtcgatggc
     7201 gacgataaat aatcagttca ctgaatttcg agagaaggcc aaagttgcta ctttggcttt
     7261 ttcttttgaa aaatagtttg aagtatcggt ttttgtttca aacaaaatat gatatgataa
     7321 caagcgacgt gaaaatgatg agaaccaata ggaggaaagc ctatggcaac aaaacgtgat
     7381 tattatgaag tcttagggct ctcaaaagga gcatcagaag atgaaataaa gaaagcttat
     7441 cgtaagcttt cgaaaaaata ccatcctgac atcaataaag aacctgatgc ggaagaaaaa
     7501 ttcaaagaag tatcagaagc atatgagatt ttaagtgacc cgcaaaaacg cgctgcatac
     7561 gaccaatacg ggcatgcagg cacagatcca aattacggtg ctggtggagc tggtggcgga
     7621 tttggtggat tcggcggctt ctctggtggc ggcttcggtg gttttgaaga tatcttcgac
     7681 tctttctttg gaggaggcgg tcggtcagtc gatccaaatg cgcctcgtca aggtgctgat
     7741 ttgcaatata cggtcgactt gtcttttgaa gaagcgattt ttggtgtaga aaaagaaatc
     7801 aagtataatc gggaagaaat ctgtcatact tgtggtggaa acggtgcaaa accaggcaca
     7861 caaccaacta cttgtcataa atgtcatggt gcaggtacga tcaatgtaga acgccaaacg
     7921 ccacttggac gagtgatgag ccgtcaaact tgtgatgttt gtcatggcac agggaaagaa
     7981 atcaaagaac cttgtccaac ctgtcatggc tcaggacatg agaagaaagc acattcagtt
     8041 aaagtgaatg taccagctgg tgtagaagaa ggacaacaaa tgcgtttagc tggtcaagga
     8101 gaagctggag aaaacggcgg accatttggt gatttgtatg tggtttttcg tgtagaagaa
     8161 agtgatatct ttgatcgtga aggttctgaa atttactatg aattgccttt aagttttgta
     8221 caagcagctt tgggggatga agtccaagta ccaactgtcc atggagatgt gaaattgaag
     8281 atccctgctg gaacacagac aggtacgaac ttccgtttac gtggaaaagg cgctccgaag
     8341 ttacgtggtg gtgcaacagg cgaccaacat gtcaaagtaa aactgatcac accgaaaaat
     8401 ctaaatgaac aacaaagaga agcattacgt gcattcgctg aagctggcgg catcaaagtt
     8461 gaagaacagc aagaagacgg tttcttcgat aagtttaaag atgcttttgg cgggaaaaag
     8521 cggaagtagt ttttctagta aaaatataaa gtagacttgc ccaaattttc tatttctagt
     8581 aaaaacgcta agtcattaat aattaaaaac cagacgaaca atcatacgtt cgcctggttt
     8641 ttttgtagtt gtcatgtttg taccaacaaa aaagccagct ttagaataag ataatcatca
     8701 tagtttctga aggtggtatc gactatgtac ttgatatcgt caatgaaccg atggtacttt
     8761 cttatgcgat ggaacaggta gagcgattgc ttgtttgtgc aggagaacaa gcgatgcggc
     8821 tggtacagtt aagtgaaaaa acaagaaact aaaaatgccc ggtcgtcatg aagcgacc
//
LOCUS       DACECZ010000102         8608 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.103, whole genome shotgun sequence.
ACCESSION   DACECZ010000102 DACECZ010000000
VERSION     DACECZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.103"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            32..102
                     /locus_tag="IWO35_002194"
     tRNA            32..102
                     /locus_tag="IWO35_002194"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:64..66,aa:Gly,seq:tcc)
     gene            115..188
                     /locus_tag="IWO35_002195"
     tRNA            115..188
                     /locus_tag="IWO35_002195"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:149..151,aa:Ile,seq:gat)
     gene            200..271
                     /locus_tag="IWO35_002196"
     tRNA            200..271
                     /locus_tag="IWO35_002196"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:233..235,aa:Glu,seq:ttc)
     gene            537..1730
                     /locus_tag="IWO35_002197"
     CDS             537..1730
                     /locus_tag="IWO35_002197"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ0317239.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1860..3332
                     /locus_tag="IWO35_002198"
     CDS             1860..3332
                     /locus_tag="IWO35_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0317240.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3415..4203
                     /locus_tag="IWO35_002199"
     CDS             3415..4203
                     /locus_tag="IWO35_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ0317241.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4270..4938)
                     /locus_tag="IWO35_002200"
     CDS             complement(4270..4938)
                     /locus_tag="IWO35_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0317242.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5053..6765
                     /locus_tag="IWO35_002201"
     CDS             5053..6765
                     /locus_tag="IWO35_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0317243.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6768..8540
                     /locus_tag="IWO35_002202"
     CDS             6768..8540
                     /locus_tag="IWO35_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0317244.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2601 a         1460 c         1902 g         2645 t
ORIGIN      
        1 tcggcagttc gattctgtcc cgcgccatta tgcgggtgta gtttagtggt aaaaccacag
       61 ccttccaagc tgttgtcgcg agttcgattc tcgtcacccg cttttagttt ttatgggcct
      121 atagctcagc tggttagagc gcacgcctga taagcgtgag gtcgatggtt cgagtccatt
      181 taggcccatt tattttattg gcccgttggt caagcggtta agacaccgcc ctttcacggc
      241 ggtaacacgg gttcgaatcc cgtacgggtc atggaagata ctttttgagt atctttttct
      301 ttttatctga aagaatgatt ttaaaaagct tagcagagtt atctttatgg atgattgtgc
      361 taagcttttt tggacagttc taatgtaaaa tatttttata ttttttaagc aaaaaaatga
      421 aaaaatacga gcagaaaact gttttatctg ctatacttgg ttcgttgcaa gttcccgaaa
      481 ggatccgcag gacggagatg ccttgtaacc gaatttaact agggggaatc attataatgg
      541 tagaaagaca cttatttaca tcagaatctg tttctgaagg acatccagat aaaattgctg
      601 accaaatcag tgatgcaatc ttggatgcaa ttttaaaaca agatccaaca gcacgagtag
      661 cgtgtgaaac gtctgtaaca actggtcttg ttttagtatt tggagaaata tctacgacgg
      721 catatgtaga tattcaaaaa gtcgtacggg aaacaataaa agaaattggt tatacacgtg
      781 caaaattcgg atttgatgga gatacagcag ctgtattggt tgcgattgat gaacaatctc
      841 ctgatattgc tcaaggagtt gatgaagcac ttgagatccg ggatgaagat aaaaaagacg
      901 tattagatga gattggtgct ggtgaccaag gtttaatgtt tgggtttgct gtagatgaaa
      961 caccagaatt gatgccttta ccaatcgctt tgagtcatcg tctggtacga cgcttggcag
     1021 atttgcgtaa atcaaatgaa ttaacgtatt tacgtccaga tgcaaaatct caagtaacgg
     1081 ttgaatatga tgatcaagga caaccggaac gcgtagatac aatcgttatt tcaacacagc
     1141 atgatgatgc agtagataat gaaacaattc gtcatgatgt catcgaaaaa gtagtgaagg
     1201 aagttattcc agctgaatta ttagatgatc aaacgaaata ttatatcaat ccgactggcc
     1261 gatttgtcat tggtggtcct caaggggatg ccggattaac aggaagaaaa atcattgttg
     1321 atacgtatgg cggttatgct cgtcatggtg gcggtgcttt ttctggtaaa gatgcgacaa
     1381 aagttgaccg ttctgccagc tatgctgcac gttatattgc taaaaacatt gttgcagcag
     1441 ggcttgctcg caaagcagaa gtacagctag cttatgcgat tggtgttgct caacctgttt
     1501 cgatctcaat caatactttc ggtactggaa cggttccgga agaagaactg attgctgcag
     1561 tgagagaaaa cttcgatctt cggccagcag gaatcatcga gatgcttgat ttgcgtcgtc
     1621 cgatttacaa acaaacagct gcttacggtc attttggtcg tacagatgta gatttgcctt
     1681 gggaacatac ggacaaagta gatgcgttga aagcaagttt agcaaaataa gttatattca
     1741 gctggaacga tcgccaagtg gtggttgttc cagctttttt tatggcagtt aacctcttga
     1801 aaaagatagt aattatttat ctatttttag atcctgtcat aatgaaaggg aggaataaga
     1861 tgaaaaaaga aacaaatgtt actttggtca cagtgagtat tttcattgca acgttcatga
     1921 cggctattga aggtacaatc gtaacaaccg ctatgccaac gattgtcggt tcattacatg
     1981 gaatggaaat catgaactgg gtattttcga tttatttgct gacaaacgct atgcttactc
     2041 cgatttacgg aaaacttgcg gataaagtgg gacggaaacc aatctttatt tttgggacat
     2101 tattgtttat tatcggttcg ttattttgcg gtttatctca aagcatgatt gggttgattg
     2161 ctgctcgtgc gattcaaggg atgggtgctg gggcaatgat gccagtagct ttgacgatca
     2221 tcggcgatct ttattctatt gaaaagcgag caaaagtttt aggtttgaat agttcagcct
     2281 gggggattgc cagtgttttt ggaccgcttg cgggaggatt catcgttgaa acgatcagct
     2341 ggcactggat cttttttatc aatgtgccga tcggaattct attaattatt ttgatttggc
     2401 tatatttaaa tgaaccaaaa atcatacatg agtctaaacc aatggatatt tttggtagtt
     2461 ttagtttgat ggccatgctt gtctcattgc tgttagggtt ccaattatta ggagacgaag
     2521 gaatgagtcc gcttgtttct gctttgctgc taggtagtat cgtaatgtta ggtatttttg
     2581 tttttgccga aaaaagagct aaagatcctg tcatcatgat gcatttattc aaaaatccac
     2641 tatttgttgt ggtcaacttg gctgcagcat tggctagtgg ttttttgatg ggaatcgatg
     2701 tttatatccc tatgtggatg caaggtgtat tagggctgaa tgcagcaatt ggtgggctgg
     2761 tattagcacc attatcacta gtctggatgg tcggttcttt ccttgcaagc aagtggatga
     2821 ctcaaatgaa aactcattgg gtcttgagaa ttggactact aatcgcagct attgggggcg
     2881 cttggttgta tcttatgcct ttcagaagta actatttatg gttttttgct atatcaacgg
     2941 tcatcggtat aggcttaggt gtgaccatga ctacctcgac tgtcagtgca caaaatagtg
     3001 tatctcgtga ccagcttggt gtagcaactt cttttaatac gttagttcga acaattggtc
     3061 aaacagtcat ggtttctatt tttggtttat tgataaatgg ggtaactaaa cgagaacttg
     3121 cagcagcaaa tttaacaaat gatgcagata tcatgaataa actggtgaat ccacaaacgg
     3181 ctaaatatat tgatgcggat attttgttac cattaagaaa aattctttat gatgggttac
     3241 attccgttta tctagttggc ttgttactcg tgatcattgc tttggtctta acgcttttcg
     3301 ttcatgaaaa aagtggtaaa ataaaacagt agtttcggtt aagatatgta cgtagccgaa
     3361 tcattcaaat ttaaagttat tcaaaaataa cttcttacta aggaaggcga aaatatggca
     3421 tttattcaag caaatattta ttcgaatgtg ttagaaatgg aagtcaatct aaatgtgata
     3481 ctaccgcaaa caacaaaaaa ggtgattggc accagttcag agcaagagac aacggatata
     3541 cctgttttat atttacttca tggaatgggt ggcaatcata gtgtatggga aagacgtacg
     3601 tcgattgaac gttatgtagc aaactacgga ttagctgtca tcatgccatc tactgatctt
     3661 ggctggtaca cagatacagt ttatgacatg aagtattgga cattcattgc agaagagcta
     3721 cctgagattt gtcatgaatt attcccgcaa ttaacaagaa aaagagaaaa aacatatgcc
     3781 gctggattat cgatgggcgg atatggcgcg ttaaaactag gccttgcaaa atcagaaaat
     3841 ttcgcagcag taatctctct ttcaggcgct gtttctgtag gaaatcgaat ggatgatctt
     3901 ttgatggttc gtaaagcatc tttttgggaa gggatctttg gaccattgga tcaaatcgaa
     3961 ggttcaaaaa atgatccgca atacttattg aaagaactag taaaaagcgg aaaaccagtt
     4021 ccaagatttt atatggcttg tggggaatct gattttcttt atcaggcaaa tcaggaaatg
     4081 gtatctgcta tgcaggaaca aggcttggcg gtcacctttg aacaaggacc tggcgatcat
     4141 aactggatct tctgggacca atggattcaa cgtgcattgg cgtggctaat ggaagaaaaa
     4201 taaaaaaaga gtcttaaaaa gatagctcct atagagatca caaacatagt gatctctata
     4261 ggagcttttt tactgctcgt tttctcttct tttattttgt tggcataagc cttcaatata
     4321 ggtttgtgca ctcgttagaa agagtgcttt ttcattcaat tctgcatggg actgcatact
     4381 ttctttaaat ccctctaatt gattttcaac tacataatta cgcataaata gaactgtttc
     4441 tttcaaaaat tcatttccgt ccataatttt ttgtcgaagc tgctgttcat gctgctgatc
     4501 cattaactgt ccaatcaata aattgtggag agaggcaatg gcctgatgac tttgggtgac
     4561 tgaaaatcct tcacgaacaa gtaattggat catttgctcc aaatgttcta atcgactcgg
     4621 aaaagcgggc acagtattga tttctaattc tgccccacaa gggaaacgtg tataaaggtc
     4681 atagtaattt tccatatact gcagcaactg ttcattccaa ggcaaagttg gatctggaag
     4741 cgctaattct tcttccattg tttcagccat tttttgcaag agctgttgtt tgtttttgaa
     4801 ataccaatat aaagcaggtg cttgaacatg aattctgttt gcgatgtttc gcatggaaag
     4861 ctgcgaaaga gcttgtgttt cagccaggat atcaaaggcg gcctgaatga tgattgcttg
     4921 agaaagcttt atttccattt tatcaacctc gtgtattgca ttatcttttt aaacatggta
     4981 aattgttagt cggtctaatt ttacagtgtt aaattagtcc tgtcaattta aacaaagggg
     5041 tgtaatcaac gaatgaaatt aatcttacga catgcgaaaa aatataaagt agctgttttt
     5101 atttccttgc tgtccgtagc agtcatggtt gcagcagcat tatggcaacc taagttacta
     5161 cagcaagtat tggaagctat tattacagaa gataatgatg aaatgaaaac aattggtatt
     5221 tacctgatca gtctggcttt gctaggatta gccgcaggtg taacgaacac gattttctct
     5281 gcaaaagtag ctcaaggcgt aagtgcagat atccgagaag aagcatttcg aaagatccag
     5341 acattttcat ttggaaatat cgaacagttc tctgctggaa atcttgttgt tcgtctaaca
     5401 aatgatatca cgcaaatcca aaatttagtg atgatttctt tgcaatcact ttttcgaatt
     5461 ccattcttat ttattggtgc cttcatttta gctatgatca cgatgccgca attatggtgg
     5521 atcatcgtag cgcttatcgt tacggtattt ctgattacgg cactttcatt tacaaggatg
     5581 ggcaagcact tcatgatcat tcaaaaactg attgatcgtg tgaatagcat tgcaaaagaa
     5641 aacttactag ggattcgtgt agtgaaatct tttgtacaag aagaaaacca attgaagtca
     5701 ttcagcaaag taagtgagga cttaacaaaa cacaacatca tagtcggtac attattttct
     5761 gtgatgatcc cgtccttcat gctggctgcc aatttggctg tggttggggc gatctttttt
     5821 gtgagcgatc ttgttaaaga tgatcctacg ttaattggtg gaattgcttc attcatgaac
     5881 tacttgatgc agattatgat ggcaatcatt attggcggaa tgatgatgat gatgacttca
     5941 agagcagccg tatcgctgaa gcggattgcg gaaattttag atgctgaacc agatcttact
     6001 tatttagacg ttccagaaca agagttgacg ggttctgtca cttttgacca tgtttctttt
     6061 cgttatcctg gtgaggatac agatacgctt aaagatatca gcttttctat caaacctgga
     6121 gaaatggttg gtatcgtcgg ggcgactggt gccgggaagt caacactagc acaattgatc
     6181 cctcgtctat ttgatccgac agaaggcaaa gttgaagtag gtggtgtaga tcttcgtcaa
     6241 gtaaatgaaa agagtttaag acaaacggtt tcctttgttt tgcaaaaagc aattttattc
     6301 tcaggaacga ttgctcaaaa cttgcgccaa gggaaaaaga atgcaacaga aaaagagatg
     6361 gaacatgctt cttctattgc tcaagcaaaa gaattcatcg aaaagttatc tgatcgttac
     6421 gaagcaccaa tagaagaacg tagcagtaac ttttctggtg gtcaaaagca acgtttatcc
     6481 attactcgtg gcgttatcgg taatccaaag atcttgatcc tagatgacag tacgagtgca
     6541 ctggatgctc gctctgaaaa gctggtccga gaagcactcg atcgagattt gaagggaaca
     6601 acgacgattg tcatcgcaca aaaaattgct tctgttgtca aagcagaccg catcttagta
     6661 ttggatgaag gcagattagt cggcgaagga acccatgagg aattggtagc tggaaaccgt
     6721 gtctatcagg aaatctttga aactcaaaaa ggaacggagg agtaagtatg actgatctaa
     6781 taaaagcaag taaatttttc tatcactatt tgaagcggta caaactctcc tttttcttta
     6841 tctttatcac agtgatcatt gctacctatc tacaagtaaa ggcacctcaa tacatcgggg
     6901 aagcttttca agaattagct aattacgtag gaggcttgat gcaaggcgta gacgataaaa
     6961 gcaaatttgt ttctgtaata tggaaactct tgctgtttta tgtactagct agtgcagcca
     7021 actttatcta tagtattttg ttcacacaag tagtagggaa gtcaacgaac cgaatgcgta
     7081 tcggtctatt caacaaattg gaaaaattga cgatccgttt ctttgattct catcaagacg
     7141 gtgaaatttt aagtcgtttt accagtgact tagataatat tcaaaatagt ttgaaccaag
     7201 ctttgcttca agtcatcacg aatgctgttt tacttgtcgg gatcttgatc atgatgttcc
     7261 gccaaaatgt agagttagct tgggctacaa ttgcttccac tccagtagcc gttttgatag
     7321 ctgtcttgat tatccgcaaa gcacggaaat atgtggatct tcagcaagat gaagtgggga
     7381 aactaaatgg ttatatggat gaaaaaatca gcggacaacg tgtcatcatc acaaatggct
     7441 tacaagaaga aacgattgaa ggattcttag agcataatca aagtgttcgc gaagcaacct
     7501 tcaaaggaca agcttattct ggtctactat ttccgatgat gcaaggaatg tcactggtca
     7561 atacagcgat agttatcttt ttcggcggct ggttagcatt aaatggtgat ttggaacgtt
     7621 cggtggcatt aggcttagtt gtgacattcg tccagtattc acaacaatat taccaaccac
     7681 tgatgcagat ttcatctgga tacagcatga tccaattagc gattacaggg gcaagacgat
     7741 tgaatgaaat atttgatgaa aaagatgaag tcaaaccaga aaacggtaaa gcatttgatg
     7801 gtgttgaaaa aggtcttgcg ttgcagcacg tagattttgg ttattctcca gaaaaactag
     7861 tcttgaaaga tgtctcgatc aatgtggaca aaggagaaat ggttgctctt gttggtccaa
     7921 caggatcggg gaaaacgacg atcatgaatc tgctaaaccg tttttacgat gtcaatgatg
     7981 gttcagtagt catcgatgga atagatatcc gagattatga tcttgatgca ttacgtagac
     8041 atgtaggaat cgttttgcaa gaatctgtat tgttctccgg aacgatcaaa gacaatatca
     8101 cattcggcaa accggacgct tcagatgaag aagtcatcaa tgcagcaaaa caagcgaata
     8161 ttcatgactt catcatgacg ttagaaaaag gttatgatac agagatatcc gaagaaaata
     8221 acttattcag tacaggtcaa aagcagttga tcagtattgc tcgaacgatc atcacaaatc
     8281 catctttatt gattttggat gaagcaacaa gtaatgtaga cacggtaaca gaagcaagga
     8341 tccaaaaagc aatggatgaa gcaatcaaag gaagaaccag ctttgtgatt gctcaccggc
     8401 tgaaaacaat cttgaatgca gatagaatcg ttgtcttgcg tgatggggaa gtcattgaag
     8461 aaggcagtca tcatgagtta ttagcagaaa atggatttta tgcggaactt tatcataatc
     8521 aatttgtatt tgaaagttaa attaaaagaa cctcgttata acggggttct ttttatatat
     8581 tttatgttgt ttgtttttat aaggggca
//
LOCUS       DACECZ010000103         8546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.104, whole genome shotgun sequence.
ACCESSION   DACECZ010000103 DACECZ010000000
VERSION     DACECZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.104"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(235..912)
                     /gene="nth"
                     /locus_tag="IWO35_002203"
     CDS             complement(235..912)
                     /gene="nth"
                     /locus_tag="IWO35_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ0317245.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(919..1617)
                     /locus_tag="IWO35_002204"
     CDS             complement(919..1617)
                     /locus_tag="IWO35_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ0317246.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1885..3069
                     /locus_tag="IWO35_002205"
     CDS             1885..3069
                     /locus_tag="IWO35_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ0317247.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3075..3518)
                     /locus_tag="IWO35_002206"
     CDS             complement(3075..3518)
                     /locus_tag="IWO35_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0317248.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3744..4031)
                     /locus_tag="IWO35_002207"
     CDS             complement(3744..4031)
                     /locus_tag="IWO35_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317249.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4202..5554)
                     /locus_tag="IWO35_002208"
     CDS             complement(4202..5554)
                     /locus_tag="IWO35_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ0317250.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5827..6273)
                     /locus_tag="IWO35_002209"
     CDS             complement(5827..6273)
                     /locus_tag="IWO35_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0317251.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6509..7435)
                     /gene="cysK"
                     /locus_tag="IWO35_002210"
     CDS             complement(6509..7435)
                     /gene="cysK"
                     /locus_tag="IWO35_002210"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ0317252.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7575..8462)
                     /locus_tag="IWO35_002211"
     CDS             complement(7575..8462)
                     /locus_tag="IWO35_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0317253.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2325 a         1752 c         1421 g         3048 t
ORIGIN      
        1 tttggaatac tggacttatt ttataggtat attggaaggc attattttct acatcgggta
       61 ttttcccaat aaaaataaca acttttattt ttatcgcaat aaattgtaat aagttaccat
      121 cctagctact gagtatgtac atttactttt tcatgcaaaa aagataaacg aaacagcttt
      181 tctccataat aacaagataa ttcatacatt tatttgcaat taaagaaacc acgtctacag
      241 gcgtggtttc ttttttagcg ccttttcttt tagacgcatc cgatttttcc catcttgaca
      301 tattgatagc aaaggacata cctcgcactt gggatttctt gccgtgcaat gatatctacc
      361 aaaaaaaatc agtgtatgat gtgtctttac ccataattct tgtggtactt tccgcatcag
      421 tgtttcttct acttccatga ccgtagcatc tagtttgcag atcctcaggc gttttgagac
      481 acgttccacg tgggtatcta ctgcgattgc tggtatcccg aatgcatccc ccaatacgac
      541 atttgctgtc tttctgccta caccaggaag agacatcaat tcttctcttg acgttggcac
      601 ttgcccatcg aaacgttcaa tcagctgctg tgcacacgct ttgatgtttt ttgctttatt
      661 tcgatacaaa ccaatcgttt ttattttaag aatgatttca tcgattgaag cttctgctaa
      721 cgcatcgggt gtcggaaaag aggcaaacaa atctggcgtt gctttattca ctgatacatc
      781 tgtcgcctgg gcacttaaga tcactgcaat caataactca aatggattat tgtgcttgag
      841 ttctccatgt gcttcgggaa acattccata catcgtttct agagcttcca ttgttttttg
      901 tttgcttaac atattccgct actcctccaa ccaatttttc agtgagactt tcggcaattc
      961 ttcttgttga gctgactgtt ctacttctct ttgaagaagc tgctgttttc ttcgtttttg
     1021 gtcttcttcc acttgttgtt ttgtacgtag attttttctt tcccacgata gcaaaatgcg
     1081 gtcaacgtaa ttaaaactat aagcctgatt taatacggct tcccgcagtg ctagcttcaa
     1141 aatttccggc tgataatggt cttcttctag ccattgtccg atccgctgga actcgatcga
     1201 agaaagcggg cgtccaaatt cttgttcaaa taattgatac actgactgga tttctttttc
     1261 ttgtgttttt tcttcgtatt ttttttcttg tgtttttaaa tactcaccta atagatcata
     1321 gatggggtaa agattatatc gatctgcttt tttaccgttg acaatcgtcg tttctatctt
     1381 gataaatcca tttgttacga gacgatccag tatttgaaaa atctgatctt gtttgattcc
     1441 catatccata gcaatcatct gcaaatcagg gaaagtatct ccttcaagct gtgccatatg
     1501 caactgtaga acaaataaaa attcttcaga ttgtagaccg atcctatgat aatttttaag
     1561 taaaagatta gatatgactg tttgtccagc gcttaagtaa ttttctaaat tcaacatgat
     1621 ttctcctccg ctcaatcaca gtatacgtaa aaaaaacatg ggaagcaaga acaggtttct
     1681 tttctattta cttcactgat cggtcagatt tacaatgtcg tctacttgat ttttgtttgt
     1741 tcatgttttt gatgcgtatc ttcacttttc ggaataaata aattaagtag atttcgctgt
     1801 tccttagaat gttgacaagg aaacaaaatt actatacact atttttgttt ccaagttaac
     1861 attattgcat aaaggagttt ttacatgtac agtttcacaa ataaacattc taaagttttt
     1921 ggatacgtct ctgtcttatt cactggatta ggatttacta tcgtcagtcc tgtacttccg
     1981 tttttaacgc tgccttattc tcactccata catcagcaag ctttctatat cactttgctc
     2041 atgtctgttt atgctctagc tgccttctta tctgccccca ttctcggtag cttaagtgat
     2101 cattttgggc gtcatccgat ccttattatc agtttattag gatcaagtat cggatatttg
     2161 ctttttggtt taggaaattc gatatggatg ttgttccttg gtcgtatcat tgaaggattg
     2221 acggcaggag aaatctccac cttatatgct tactttgccg acatcacgga acctaatgaa
     2281 agaaccaaag tttttggatg gatgggtgct ttagtcggta taggaacaac gctgggtcct
     2341 attatagggg gtctattagc tgagttgggg aattctgttc ctatatttat tggagcactc
     2401 tttactttct taaatgccgt ttatggctac acatttatgc tagaaagcct tccaataaaa
     2461 aaacgttcag tagatttaag tttctcccat gtaagacctt ttcaccaatt acaacaatta
     2521 ttcaagattt catccgttat tcctttattg acagcaggtt ttgctgtttg gttggcagct
     2581 ggctctcttc aatctatctt ttctcaattt tcgattgacg cctttcagtg gaaggctggg
     2641 ttggttggtt taagcttctc tttgattgga atcttggata ctgtctcgca attgttcatt
     2701 atgcctcgat tgctcaaaaa attttcagaa cagcagatca caagaatagg gatgttcagt
     2761 gaaatcctcg cctatctatt catcacatta tctggaattc ttctactgcc gattttgttt
     2821 ttatttggga ttatctgtta tggtttcggt gattctattt ttactcctgt attcaatggc
     2881 caactatcca attctgtctc agataaccag caaggtcttg taatgggcgg tacccaaagt
     2941 atacaatctc tatcgcgcgt catcggcccg ctaatcgcag gacagcttta tgctgcagca
     3001 ccatttcttc cgacggtatt cggatttttg cttatgattt ttgcctatta tcactacaat
     3061 aaaaaatagt agcttcattt gctacttcgt ataaattctc tgtatcgacc gataaaacga
     3121 aatactgtgt caaattcttc ttttgacata ttttcaaata cagtagcatc tcgctgtacc
     3181 catttttgat ggagttgctc gtgtctattc cttatttttt gtccgattgg cgtcaattga
     3241 aaatagattt cttttttatt ctcttcgctt tgataagtgt ttatgatccc tctagctatc
     3301 agtttttttg ttagcttact gatggctcct ctagtcatgt aactagcatt cgctaatttt
     3361 gtcacgttag gatcagggat tttcccgata tgttcaatca cttctagttc atttagagta
     3421 aatcctgcaa attctttttc catttctggt ttattacgat gaataatctc attagctaaa
     3481 tccagatatt cttctattag cagttgtctt ttatccattt ctacaccttc tttttctttc
     3541 attatattgt ttttttgttt cctattcaac aaagataaaa atattttgta ggaaaaccca
     3601 atctgaaacc aagaaaaaaa ggggttgaca aagcttgtca taaccccttt ttctgattaa
     3661 aagatagtaa agagacagtc atcggtaatt gtgatgattt tttgtcatca cttaaggatg
     3721 agcggccttt tcaaaaactg tctttaaaga tcatcatcag tttctagcac atatggctga
     3781 ccttcatatg tgtactctgt attcaatgtg ccgtcatttt catctgagta gatgcgtcta
     3841 ctgaaaggaa aacgatgaga aacattatct ttggcgtcat ttgctggcat cgtgtaatat
     3901 tcctcttgcg tttcattcaa ctgagagagt acttgatctg ccaactcatc aatattgatc
     3961 gttaatttct tcgaagcact tttataatct tctaaaaatg catcttgttg gacaaaatat
     4021 tctttattca tcttctttct cacctttctt ttcaaaatca aataaagctg cttgttcttt
     4081 tatgtaacag ctttatcttt gctgcttatt gtgacaaaaa attcgaagaa agacaactaa
     4141 ttgtactttt cacaaaaaaa gacagagggt cgtgacaaaa ttgtgtcaca accctttttc
     4201 tttattgtgc agtttcgatt tcgcctagcg ctgcttgtgc aagtaggttc aagtaattcc
     4261 atgggcggtc aaaatgtggt tggaagaaga aatctgaaag tgcgagatct tctacagtca
     4321 ttttattttg aacagctaaa gacatggtat ttgctgattg tgtaatgtcg tatttggaca
     4381 tgaactgagc acctacgata cgattcgttc ctttttcata gactagctcc atcattactt
     4441 tttcagtagt tggcatgaac tctggacgat aattatcttc aaatacagtc gcttggactt
     4501 ctagatcgtt caaagttgcg ctttctttgg tcacacctgt cgaaccgatc gtccagccaa
     4561 ataggtaaag tcctgaagtt ccttgagtac cacgatacgc cattttatga tctgtcaagt
     4621 tatttcccac aagcaaacct tgacgaacgg catttgttgc caaaggaata tagttttgcg
     4681 tgccacttgg attgtagttg actactgcag agtctccagc tgcaaaaatg tctggattac
     4741 ttgtctgcat gtattcattc actttgattg ccccgtttgg cagtgtatct acttttcctt
     4801 cgatcagttt tgtatttgga cggaagccca cacataaaat gaccatatct gtatcaaatg
     4861 tatctgttgg tgtagctact tgtttcactt ttccttcttc atctgtgata aattcagtaa
     4921 cattttcgcc tagcgcaaga gtaacaccgc ggtctgtcaa ttctttttct aagatatctg
     4981 tgaatggttt atctagatat ttattcaaga tgcgatctaa tccatcaatc aatgtcactt
     5041 gttttccaga ttcgacaaaa gcttctacta attcaatacc gatatatcca ccaccaacaa
     5101 ttactacacg ttttgcttct ttcgcttgtg caataatttc atttgcttga ttgtagtttt
     5161 tgcataggag tacatttttc gcatcgattc cactaatcgg tgggataata ggccatgaac
     5221 cagttgtcat cactaattta tcataagaaa ccgtttctgt ttctcctgtt ttcagatctt
     5281 ttgccgttac tgttttattt tctgtatcga cgtctgttac atcgtgttcc attttaactg
     5341 ttgctcctaa tgaagataat tcttcaggat ttgagtagaa caagcctgct gggtccttta
     5401 ctactccgcc gacataaagt gcgataccgc aagataagaa agaaatattg tcatttcttt
     5461 catatactgt cacttctgct tctggatgat tttttaaaat actttttaca gctgcagtac
     5521 ctgcatgagt acagccaatt actactactt tcatttatgg tccctccgat taacattttg
     5581 tccttcgaca aaactactat aacagaaata aaacggatac aacagtttaa tgcttgtgaa
     5641 ttcacaaagg tttgtttgta gtttcattcc ttttcaagca taagaaccca atatctacaa
     5701 gattcttatt ttaataatat tcacttttat atctaagatt gtgaataaaa gacaaaaaaa
     5761 taaaaaagct agcgtttttt ttacacagct agcttttttt aggtataaca cttgtagaaa
     5821 ctgtttttac atttgcgcct tttttgctga ctctttacaa tccggacaga gtccatacaa
     5881 ttccaaccga tgttcattaa tctcaaaatc agtcagtctg cttgcagcca tttctatatc
     5941 ttcaagccct gggtaatgaa aatcaacaat cttaccgcag ttttcacaaa ttacatgata
     6001 atgacgcttt gaactgaaat caaatcggct agaagcatcg ccgtacttca tttcttgaac
     6061 aaatccaatc gaagtaaata agcgaaggtt attgtaaaca gtcgcaacac tcatatttgg
     6121 aaaccgatgt tcaagtgctt tataaatctc atcagccgtt ggatgggaat gatgttcaat
     6181 caaatattct aaaaccgcgt accgttgagg tgtgattcga atgttctctt tttttaactt
     6241 ttcgataacc tcttcgacta acttattttc catgtaaaca ccccacgttg ttattctcgt
     6301 cttatcatac tatttttaca ttttaaaatc cacttaacat gaaaagaaaa ttcaatttta
     6361 ttgtatttta atgaaaaaag acgtaaaaca gtcgcgttca ctattctacg tctctattca
     6421 aataattttt ctcatcataa atttttgtgt gttcttttca tctttaaaag ttttttacat
     6481 aaacctatct ggagaaaaac caagtgcctt atatctcttg gtaaagggct gtagataaat
     6541 aacgttcacc attgtcggga accacagcta ataccttctt gccttttcct aattcttttg
     6601 cgacttttag cgcggcagca attgcagcgc cagatgagat gcctactaaa atcccttctt
     6661 ttcgcccaac ttctctagct gtttccatcg cttcgtctcc actaatagaa aggactttat
     6721 catacacaga agtatccagt gttttaggaa caaaacctgt accgattcct tggattttgt
     6781 gaggtcccgc ttctttccct tctaaaatag cagattctgc tggttctacc ccgattaatt
     6841 cgattttcgg atacacacgt tttaattctc ggccagcgcc agtgatcgtt cctcctgtac
     6901 cgatacctgc aacaaacgcg tccaagccat ttactccaaa tgcttgatgt atctcaggac
     6961 cagtcgtctt ctcatgcaca agaggatttg cttcattttc aaattgtaat ggtaaaaagt
     7021 agccgttttc ttttgccaaa cgttctgctt ctctgattga tccagaaata ccatccgcac
     7081 caggagtcaa aatcaactct gcgccgtacc ctttcatcaa taaacgtctt tcgatactca
     7141 tcgtctcagg catcacgatg attactttat atccttttgc aactcctacc atcgacaatc
     7201 cgattcctgt atttccagaa gtaggttcaa tgatagtatc acctggtttc agtagaccat
     7261 cgtgttctgc tttttctatc atacttaaag caatacgatc ttttacactt cctcctggat
     7321 taaaaaactc aagctttaca aaaacatctg ctgaatcttc tggtacgatt ttatttaatt
     7381 tgacgattgg cgttttgcca atcaattcgg tgactgagtt atagatagct gtcatcttca
     7441 attcctcctt gtctttgctc ttcttactta tcataataca atctgttcat ttatggcgag
     7501 tgattcgtct ttgactagca aaaaaaagac aaagatatac catatatggt aatttctttg
     7561 cctttttatc taaatcaaac gtttagttct tgaccaacta ataaagaata actggacata
     7621 gacatgccat ttttcgccat tagatcatca acagacatgc caaatttttg tgcaatatcc
     7681 catagcgtat cacctgacac gactgtatat gtctgaccat tatttgtatt tacatctgtt
     7741 gttgaagatg atgacgttga agtcgtcgta gtagtcgttg ttgttgtact tgtggttgtt
     7801 gtagccgaac ctgttgatgg cgtatcaaaa cgggttaaat tataagtatt aatcaatgag
     7861 ttcaatttgg ccccataatt tggatcggta gcataacgac ctgtcaagta cgctgttgca
     7921 tcataaaaac ttgttgtatt gcttttccac acgccagcat aatgataatc gcccgtggaa
     7981 aaactggttg aacgaagaac attcgcatgg tcttggaagg attcccaata agaagaatat
     8041 tttcggaatg gctcattcat cgttacccat tgtccgttta catattcttt ggttgccata
     8101 taaacgacct gtccgttata gcttcctttc acaccgaata gattataatt tggcgcacta
     8161 gctaatccgg aagtgccata tgcactttcc aacaacgctt gagcgatcat gactgaagca
     8221 tacaagtcat tacttgcagc aacttctgac gcagaccagc cgatttgatc gataaacgct
     8281 tgaggatcag cgactgtttg agatccttgt tcatctgcat gaacatgttg cggtaccgct
     8341 gtaaccatca tcggcccaac taaaagactc gttccaatta aaggagcaat cgctttttta
     8401 ttgatcacca tttttctttt aggtctatgg tgccgggcac tgcgggtctt cagctcttgc
     8461 attcaaattc ctcctcaaaa taaaaaaact taataaaaag ttctcttctt aaaacaaact
     8521 taattaatta aattatactt ttaatt
//
LOCUS       DACECZ010000104         8499 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.105, whole genome shotgun sequence.
ACCESSION   DACECZ010000104 DACECZ010000000
VERSION     DACECZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.105"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(43..1743)
                     /locus_tag="IWO35_002212"
     CDS             complement(43..1743)
                     /locus_tag="IWO35_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317254.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            2250..3410
                     /locus_tag="IWO35_002213"
     CDS             2250..3410
                     /locus_tag="IWO35_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0317255.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            3426..3869
                     /locus_tag="IWO35_002214"
     CDS             3426..3869
                     /locus_tag="IWO35_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0317256.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(4352..4882)
                     /locus_tag="IWO35_002215"
     CDS             complement(4352..4882)
                     /locus_tag="IWO35_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ0317257.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            4987..5538
                     /locus_tag="IWO35_002216"
     CDS             4987..5538
                     /locus_tag="IWO35_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0317258.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(5691..6164)
                     /locus_tag="IWO35_002217"
     CDS             complement(5691..6164)
                     /locus_tag="IWO35_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0317259.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            6302..7774
                     /locus_tag="IWO35_002218"
     CDS             6302..7774
                     /locus_tag="IWO35_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0317260.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            7771..8481
                     /locus_tag="IWO35_002219"
     CDS             7771..8481
                     /locus_tag="IWO35_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ0317261.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         2793 a         1491 c         1647 g         2568 t
ORIGIN      
        1 cgtttggaga gttttttgat cggatgtatt tatccctttt gcctaagcac gtaaaagctt
       61 gtatctatga taaataaata aacatagcaa ggtaaaccag ccaatcaaag taagagaaag
      121 aatgaaatag aaatagattg gcgtttgata atgaagcgtg agctcgttga ttcctttttg
      181 ctgcttcacg attggggtcc cgatatctgt tacttcataa tcagtcaatg tttttccatt
      241 ttgctgtagc acagtccgat cgtagacgat aacagggata tgaacagttt tgtccccagt
      301 gtttttccac ttgattgtca aaagaccgcc tggatgtgtt tttataaaag ttgtttcgtt
      361 ttctagtatt tcttcgttat accggtcgta tttattgtct tttgtctcct tatagattgg
      421 cagatagtca ggtgtggttt tttggaaaac gaacaagctg gtagacatat ctttgctgaa
      481 gaatgttttc cttgcttctt ccggcgtatc gaatagataa gtatgtcgac tgacaaatgt
      541 ttcattttcc cacttttcca gatgctggta taagttctgg atcgtctgtg ctgaaaaaat
      601 aagaatgcca aatatactta aaagacggaa ccacttttgt tcagaccact ggttcagtga
      661 aagggcaagg taaagtagaa gcaatagcgt aaatggcacg aaaaatcgaa atggaaactg
      721 gatcaaacta ataaaaggca catttttttc gatcaatgtc gtccatggaa tcaagctagt
      781 agaaagaaaa aagaaaacaa gacttgtgta caacacgatt ttttggaata aggtgttctt
      841 ttgttgaaag aaaataaaaa gcaaaaaacc tgataatatc aatataggaa aggccttagg
      901 gtaatggagc caataactgc catccagatc tatcgccttt tgactcattt ctctgttaac
      961 aaagggagga agaatcttat tcccactgta aacaatagct aatcctgccc aaatatttgc
     1021 tgtcagaaca aaataaagca agaccgactt aatcagttga agcagtgctt ttcctttttc
     1081 agtctggtta aaaaagtaat aaagataaaa agggatatac atcaaagcta acatgatcga
     1141 agacagtaaa tggatctgtg tcataagtcc cactgagaca gcgactttga taacagggaa
     1201 ctttttatga aggacgaaat cagcaatagg gatcatacat aaaggataaa aacaagtccc
     1261 ccagctagtg aatccttggt tgatagacca gtattgaata gaatacgtac tcatgaataa
     1321 aacggctact ggtacactta cccaatttcg aacattgaca tatctcagta aaatcagcat
     1381 agaaaatcct gaaataagat aaagaatgaa atttgaaaga atctgataat gaaaccagct
     1441 acctgcaagc aacacgatca atccttgtaa ataagcgaaa aatgggccgt aaacggcatt
     1501 cacgatcctg ccactttgct ggaatccata aagggaagga aaataatgga aatccccata
     1561 tttaatttgt tgcgcggcat catagaaccg attgtaatgg aatggggcat cagcacccaa
     1621 aattagttta tgtgtgaata aatgaggcat gagaaatgcc aaagcaatga aaaaaattgc
     1681 taaaaatggg ccatattttt tcaattgtcg aactcctttg caaactttag ataaattcat
     1741 catatatcct atgcgagagg taggcaagaa attagaaaat tcatgaaaat atttatgaat
     1801 tttacaagaa aaacataagt gaaagttcta aggagagagg atgcgacata agttttgttg
     1861 catctccttg ttttgaaaca gaatttttct taatactcga aacaaagtag ctttttcatg
     1921 acctctcggt aaacggtgtt caatcagctg cttctcggta ataagctcac tagcctagaa
     1981 aatatgagaa gctattttct gactagcgct cttattactc aaagcataac gcagttttca
     2041 caacctcttc cttaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaacccaac
     2101 aaaaaatatg agtagctatt tttttgggct gtacttatta cttgatgcag gacagctttt
     2161 tcacaacctc atcggctttc attttctatt tttttcctga acccatataa taaaagaaag
     2221 aacaaaaaaa cttgcaaggg aggacacatg tgaagcagag taagcaaaaa cagtctcgtc
     2281 gacttttgtt tcgtttgttt ttattaatat gtatcgtcgt agggttactg atcgctttat
     2341 atccatttta tgtggattct ttaaatagtt ttattgatca aaaacgtatc gaacaagtac
     2401 aaaaacggac cgcagctgaa attgaagtgc agcgaaagaa aatggaggag aaaaaccaac
     2461 gattaactga ccaaggattc aatcctggta ctgatccttt caatgaaaag aacagaaatg
     2521 aaagtacaac atccagccaa ttagaggaat ggctgatcgg aagtatcaat atacctaaga
     2581 tccaattaaa tatttcactt tatgatcggc tcaatggtag gatccttgaa aatggagcag
     2641 gcgtcttgca aggaacctct ttcccgcttg gtggaaattc gacgcattca gtgatctctg
     2701 ctcacagcgg tttacctaat cgacggttat ttaccgaatt agatcgttta gaaaacggag
     2761 acacgtttat tttgactgtt ttgggggaga aattggctta tcaagttaaa aatatacaag
     2821 tggtactgcc agatgacacg tctgttctaa cgatttcaga aggagaagac ttagttacct
     2881 tgcttacatg tacgccgtat atgatcaaca cgcatcgttt gctagtgaca ggacatcgga
     2941 ttccttataa cgaatcggta aaaaaagaag aaggaaaagg aaatcaggaa agaaccatgc
     3001 ggcaatggtt tattcttgca ggaacgataa tagctgtagt catactgtta gtgtttatcg
     3061 gacgactaat ctatcagtat agattaagta aaaaagtgct tgatttttca tttattatta
     3121 gtgattcgtc agggaaaccg gtaaatggta gttcattcat tttgaaacat aagaaaaaaa
     3181 cacttactag aaatggtgtg ccgttttcag tacaaagcga ccatcatggg aaagtcaagc
     3241 tggatcaatt gcctggtgga acttatcgaa tcgtttcagc tgatgatcct aaagttgctg
     3301 tttcttttgg tattagaaaa gtaaaacaag agaagatata cttttttgag gggcgaaaac
     3361 ttgtaaaaga gcttcaaaaa aatggttttt attttaaaat caatgaataa gtaaggagag
     3421 aaaaaatgat ccgcacaaca acaaaattgt ctgccaaaga actggaatct gttctatcga
     3481 tttggctgca tgctaacttg gattctcatg aattcattcc atcaacttat tgggagaatc
     3541 aagtagaatc tgttaggaaa gctttaccag atgcagtgat ttacttagca gaaatagatc
     3601 aagaaattat tggttttgct ggggtgattg atcagtttat cgcaggaatc tttgtgaaaa
     3661 gggaataccg gcaacaaaga atcggtacgc aactaatgaa aaaaataaaa gaaaccaata
     3721 aaacactgac tttgacagtc tatcaaaaaa atcaatcagc tattcatttc tatctttcac
     3781 aaggttttac aattcaaaca cagcaaaaag atgaagcagt aaatgaggag gaacttgtga
     3841 tgaaatggaa tcaaaacgct gatttttgat gaacagaata acgaaataat gctcccagcc
     3901 taattttttg ctgggagcat tatttcattt tgaagtcact aatttaactg tgtgaatatg
     3961 cagttgttac aatctttgat aaacggtgtt caatcagctg accctcggta ttagctcaaa
     4021 aaacggcaaa atatgaggaa ctatttttcc atttgtttct ctaatactca ggtcaaaacg
     4081 ctgatttcac aacctcttga taaacggtgt tcaaacggct gcttctcggt aataagccca
     4141 ctagcctaga aaatatgaaa agctattttc taactagcgt tcttattact caaagcataa
     4201 cgctgatttc acaacctctt gataaacggt gttcaatcag ctgaccctcg gtattagctc
     4261 aaaaaacggc aaaacatgag gaactatttt tccgtttctt tctctaatac tcaggtcaga
     4321 acgctgattt cacaacctct ctttcacaac cttattttac tcgtttataa ttttcatcga
     4381 agtattttcc gctacggata tcatctggca ttccatcgta tggttcttcg ctaataacat
     4441 cttcattgtc tatacctgca tcacccatat aagtgatcgt agcaaattca ggaacaagta
     4501 attttggata agtttcatat ttttcacgag ctgcttccat ttctgcgata tgttcgtttt
     4561 ggaaagtaac agtgatttct ggatgttctt caacccattt agggaaaaag atcattccgt
     4621 gaagtttctt ttcatttggc ataaagtcca atgccacgtc tgtccctgtt ggctctgtcc
     4681 aagtgatttt gaatacgccg tccgtcaatt taacgatatc agcttcttgg tcttttaccc
     4741 aacggccttt gaccattccg ccatggatac ggtaatcaac agtatggtca tttttggcat
     4801 accattcata ttcccaacca ttgtcatacg tatagataaa gtgagttcca ataaaatctt
     4861 ccaattcttt aaattctttc atgtttaagc atctccttat aaattaaaac atgtaataaa
     4921 ctacatgttt tcgattactc atttatgata gaatggattt aaagatttgt caaggaggga
     4981 aaactgatgg caagaaaaaa tacacttcga tatattttgt tagggttatt aagtaagaag
     5041 aagatgacag gatatgaatt gaatcaatcg ttcaaaaatg aaataggaga attttggcaa
     5101 gcaaaacata gtcagattta tcctgaactt gcaaaaatgg aagagcaagg aatcattcag
     5161 catcaagtag agatcacagg tgaaaaattg gaaaagaaag tttatgaatt aacagaagaa
     5221 gggaaggaac aattggcaga gtggattcat acacccacca atgaactacc ggttaatcga
     5281 gacgaatttg ttctgaaatt gtatttcgtc aaagacgtga atgatccggc attagtagaa
     5341 attattcagc agcagcgtga ccttcatgaa gaaaaacgac ttcatctatt agagcgaaaa
     5401 aaaacgatct ttcctactga aaaagaaaaa catgaaaatt acggacacta cctgatttta
     5461 gcgcatgcga tcaatcggga aacagaatat gcaaattggc taacagatgt attagaagaa
     5521 agtaaccgaa aaaaataaaa aaatatccga acagtgatgg agaattcctt tctatatgaa
     5581 agaaacgcag atcaccgttc ggatattctt atgttcaaac aggccttttt tcaagtattt
     5641 atgtagcatc tacttattaa aaagcttggt acttaggaga ttagtggcgt ttagagttct
     5701 tttaacaact gttcgtagtc agctttttca tcgacaggaa ggagcttgta agttaattcg
     5761 tctgtctcgt tgtcatgaat gatgtacatc ttagcattct cttctttgaa gttaccgatt
     5821 tgtaaaatcg tatagcgttc gtcgatcaat tcgatatctt tgattgcttt tgaaaaaact
     5881 ttacggacat ggtgcatttt cttttggcgg atttctgcct tttctgcagc ttttgacaaa
     5941 gtaaatccgt catctaaata ttttttgatc agttctactt ttatgactgt aggtaaacga
     6001 tattttctag gacaattcgc atcagcttcg atcgaacgaa tgaatccctt ttgttcccaa
     6061 taccttaatt gacgtggaga cacacctacg atatcactta attcactgat gccaacaacc
     6121 aattgatcat tttccaacaa acgtttaagg gaagccccaa ccatttttgt cactcctttt
     6181 gtcctcttgc ttcccttaat tatatccgta ttaacttatg tgtcaagtag gttgacaaat
     6241 agcgaattta ggactaaagt attctagtag tttcagaaaa aattcagaaa gaaggagaaa
     6301 tatgagtaac aaccaacccg tagatattta cggaaaacca tataatcgga cacttttagt
     6361 tgttgtctta ttaattggta cattttgtac cgttttgaat cagaccttat tgacgacagc
     6421 ttttcctaca ttgatgaaag ctttcgatat ttcagcgtct gatgtgcaat ggttaacgac
     6481 tggtttcttg ctagtcaacg ggatcatgat cccaataaca gcatggctga tcaataaatt
     6541 cagttcaaga aatttatatc tttctgccat gacgatcttt ttgatcggaa cgattacttg
     6601 ctttacagca cctaatttta gtacattact aatcggtcgt ttgatccaag cttgcggtgt
     6661 gggagtatcg atgccattat tgcaaaatat catgctatct attttcccgc cagaaaaaag
     6721 aggatcagca atggggatgt cagggatcgt tattggactt gctcctgctt taggccctac
     6781 attatctggt tatatcattg ataactacca ttggcgtgac ttgtttggta tggtactgcc
     6841 aatcgttgtt cttgtcttgg ttttagcatt cttcctaatg aaaagtgtta ttccattgtc
     6901 aaatcctagc attgatattt tatccgctat tttatcaacg attggattcg gtagtttact
     6961 ttacggattt tctagtgtag gagatgacgg ctggggaagc ttgaaagtaa ttggtttctt
     7021 agttgttggt gtgattttca tcgccttgtt ctcatggcgt caattacatt tggaacatcc
     7081 attcttggaa cttcgtgtat tcaaatcatc tacattcacg attgcagctg ttttagctgg
     7141 tgtaacaaat atggcgatgg taggggcaga aatggtcgta ccattgtata ttcaaaatat
     7201 tcgtggagaa tcagcattcc attctggctt gatgttgttg ccgggtgcat tgatcatggg
     7261 ggctatgatg cctgtgacgg gtgcgatttt cgataaatac ggcgcgaaac gtttagccat
     7321 cacagggatg ttcattttga cagcggcaac tgcaccattt gctttcttga caaaaacgac
     7381 tccagtttta tatatcgtga tcctttatgc gatccgtatg tttgggatct ccatggtcat
     7441 gatgccggta acgacttctg gtatgaatgc attgccaaat aacttgatca gtcatgggac
     7501 agcggtaaat aatacattcc gtcaaatcgc aagctcgatt ggtacagcta ttttgatcag
     7561 tgtgttgacg aatgtgacaa aagcaaatat cccaagcaaa tctgtattgg ataatacgcc
     7621 attagcttat aaagatcagg caatcaatgc cacactgtca ggttaccatg ccgcgtttat
     7681 cgtagcgatc atctttggga ttcttggctt tattattgcc tttttattga agaaaaaaga
     7741 acctgttcta atgaaagcgg gggataatgc atgattattg gagttttgat tgtcagcttg
     7801 cttgcttttg ctttaacgaa cgtgttcgct aaaaaaacat ggcaaacctt tttatctttg
     7861 attttcggac taatcttcgt cgcttcacta agtttgatcg tcgctaattt gtccaatcac
     7921 tttggaatgg aaaaagtaac ggaaacaaaa acagaaaaaa ttgtttccag tgctgattcc
     7981 caaggagcag atatgctttt gtataaagct ttagggaatg gaaaagaaaa agtgtatctt
     8041 taccgaacaa atgaaaaaca agaaaaacca aaagcaactg gtacggataa cgaaacaaat
     8101 aaagtagaaa agacagacgg cgatgctgaa aaagtgacga aaactactta ttgggaatac
     8161 aaaaacgaca tgtataaatt ctggttcaat attgctgata acaatcacga atatgacagc
     8221 cgagtgaata cattcaaaat cccagagaca tgggtagaat taagtacgga ccaagcagcc
     8281 aagttagctg aattagtgaa gaaacagcaa agtacgatgg aaagtgaagc aaaagcttat
     8341 gttcaagacg gtatggtaaa agcaatgact gaaaatccaa aaatgagcaa agcagagcaa
     8401 gaacaacgta cgaaagcctt agctgctgaa ttccaacaac aagcattcgc aaaattagtg
     8461 aaagaagcaa aaggcgaata atattttgaa caccatgta
//
LOCUS       DACECZ010000105         8465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.106, whole genome shotgun sequence.
ACCESSION   DACECZ010000105 DACECZ010000000
VERSION     DACECZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.106"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(107..604)
                     /locus_tag="IWO35_002220"
     CDS             complement(107..604)
                     /locus_tag="IWO35_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ0317262.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(734..1444)
                     /gene="budA"
                     /locus_tag="IWO35_002221"
     CDS             complement(734..1444)
                     /gene="budA"
                     /locus_tag="IWO35_002221"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ0317263.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1457..3121)
                     /gene="alsS"
                     /locus_tag="IWO35_002222"
     CDS             complement(1457..3121)
                     /gene="alsS"
                     /locus_tag="IWO35_002222"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ0317264.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3326..3988)
                     /locus_tag="IWO35_002223"
     CDS             complement(3326..3988)
                     /locus_tag="IWO35_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0317265.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3998..4813)
                     /locus_tag="IWO35_002224"
     CDS             complement(3998..4813)
                     /locus_tag="IWO35_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0317266.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(5075..5518)
                     /locus_tag="IWO35_002225"
     CDS             complement(5075..5518)
                     /locus_tag="IWO35_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ0317267.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5652..5990)
                     /locus_tag="IWO35_002226"
     CDS             complement(5652..5990)
                     /locus_tag="IWO35_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0317268.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5978..6355)
                     /locus_tag="IWO35_002227"
     CDS             complement(5978..6355)
                     /locus_tag="IWO35_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0317269.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6579..7772
                     /locus_tag="IWO35_002228"
     CDS             6579..7772
                     /locus_tag="IWO35_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0317270.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7938..8360)
                     /locus_tag="IWO35_002229"
     CDS             complement(7938..8360)
                     /locus_tag="IWO35_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0317271.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2491 a         1707 c         1421 g         2846 t
ORIGIN      
        1 ttgtgatttt ataaaaaaat aaaaaaaccg aaaaacagaa atgcccactg tctttccggc
       61 tctttttatt cttgatttct cttctttttt gtttcaatag acgattttag ttattaataa
      121 atgaaaaagc atttatatta aaatttgcta ataaaagatc tacatatttt tctagaaatg
      181 cttgatcgat ttcatcatac cctttcgtta ctccgcaatc gatatctaaa acaccgatta
      241 aattaccttc ttttaccatt ggcactacga tttctgattg tgcggctgaa tcgcaggaaa
      301 tgtaatttgc atgggttttg acatcatcaa cgatcaatgt ttcttgtttt tcagctgatt
      361 ctccacaaac tccttttccc atcttgatac gtgtgcaaga aactcgccct tggaacggtc
      421 ctaaaatcaa ttcattacca tcgaataaat aatatcctgc aaagacagtc tcaggcaaag
      481 tctcattcaa caatgcagat gaatttgcta aatttgctaa ccagttatct tctgcttcta
      541 ataatccttg ttgctgtaat aataaaattt cgtatgcatc ttttttctgt ttttctgtcc
      601 acatgataaa aaccgctcct ttttgatcta tggagaagat tatagctcag tttttttgta
      661 aacgctaggt ttcctcctca aaaaaacgag atacgtcagg ttaatcacct tgaacgcatc
      721 tcgctgtctt tttctattct gcttgttgga tttctgctgc taaatcagca taatcaatcg
      781 tttcgtgaac aaaagtcgga tcatcaactg gaaaatgctg tacaagggac gagatgcggc
      841 taatctcaac ttttccttca gccacttcaa aatccattac atgtccgcca aatgtatgtg
      901 tatcatcgat aaaatgcaag tgaaatcccg ctgcagcaac tccttggaat aattcaggcg
      961 taaagaaccc gactaatgtt ccttggacat tgctttgttg gaattctggt tgagactctg
     1021 aaatttttgc tagtcttctg taaggttttt cttgcttcgg catgatacgg atgtgcatat
     1081 ttttgaataa tccagttatc ttcactgcca aaaataggtt ttccccggct tctcttacta
     1141 atatgtcttt tttcaattgt tctccatctg cagtttttga gacagaaaaa ctagtatctg
     1201 gcgtaaaatc tgtaattgct gcataggggg taagttcact tccatctaat tgggcaactg
     1261 agccatctga tcttccttga taagctacgc catctaaaaa gatgacttca ccatcaagcc
     1321 catgcaatgt tcccaatccc agagttcctt tttctaatat atgagcaatc gtttctgttc
     1381 catccatcaa gccagccatc aaagctccta atgtcccgtg ctggtataag attttttcag
     1441 tcatgtcgac acttccttaa tagaattgat ctggtaagat tgtttctccc aatttctcat
     1501 tatcacggta atcaatcgga atatcaataa ttacgggtcc ttctgtcgca aatccttctt
     1561 ctaaagcttt ttctaattcc tctacacttg tagctcggat tcctttagcg ccaaaagctt
     1621 ctgcatattt cacaaaatca actggaccaa agtctacacc tgacgcccgc tgatatttca
     1681 ttttttcttg gaactctacc atattgtagt gtccatcatt ccaaatcaaa tgaacgatat
     1741 tcaatttttt acgtaccgcc gtttctaaat cctgggcaga gaataaaaat cccccatcgc
     1801 cggaaactga tacgatttgt gtatttggac gcaccaatgc agcagaaatc gcccatggca
     1861 atgcaacacc aagcgtttgc ataccattac tgaataacaa atgacgaggt tcataacttc
     1921 taaagtggcg tgccatccaa atataatgac tacctacatc tacggtaaca gtcatatcat
     1981 ctgttacttt tgactgaagc gtgttaataa cttctaaagg atgcaagata cctgcttctc
     2041 cttttgattg aacaatgtcc cgctccgtta attctgcttg aagagatgct aaatattctt
     2101 ttgcatcttc tgacacttgt tgaggctcta atgaacctgt caataaatca agagtggctg
     2161 agatatcacc gatcaattct ctttcaggct gcataaaagg atcgatttca gctggcgctt
     2221 catcgatcac aatgatccgc gcatcttttt ccgcattcca gtttcttgct tcatattcga
     2281 ttggatcata gccaatagca ataactagat cacttcgttt caaaagcata tctcctggtt
     2341 ggtttctaaa taaacccaca cgaccaaaga aatgcgcttc taattcacgt gagatcactc
     2401 cagctgcttg gaatgtctct acaacaggca atgctgtctt tccaactaat tgcctgatgg
     2461 ctaacgtttc tttttcactt gatcctcgca tacctactaa tagtaccggt agttttgctt
     2521 ctctaatgcg ttcagctagg taacggatat catctgcaga agcagaacct aatttaggat
     2581 cgctcaatgg cttgatgaca ttcccttgga caggtgcatc cactacatct tgcgggatac
     2641 tgataaagct cgcgcctttt tttgaactct tagccatacg ataagcattg gcaatcactt
     2701 ctgaaattgt ttctggatct tggatttctg cactgtattt tgtgattggc tggaacagtg
     2761 ctgcattatc catgctttga tgggtcaact tcaatagatc gcttcgtttt acttgtccgg
     2821 caattgctaa aactgggtca ccttctgctg tcgctgttac caaacctgta gctaagttac
     2881 tagcaccagg accacttgtt gcgataacta ctcctggctc tcctgtaata cgtccgattg
     2941 cttgtgccat aaatgcagca ttttgttcgt gacgagtcac gattaattca ggaccttgat
     3001 cctctaattc attaaataca ccatcgattt ttgcacctgg tataccgaat acataatcaa
     3061 catgatgatt aatcaaactt tctactacag atttcgaacc ttgtttcatt ttttcgtcca
     3121 ttgaacatcg tcctttttat gctttatttt taattattag ttagaaataa tcacaaataa
     3181 ttgatacagt ttctaaacct gtttcattca aaagtatagc acgttttatt tcatttgttt
     3241 agccatatgt ttcaaaaaat gaaaaagaat gacagttttt acataaaaac tgtcattctt
     3301 aattgaaagt ttcaatttat aaagtctaca gatccatagt tctcatcaat ttacgattgt
     3361 tgaagtaaat caaaccaaat acgatagaag gacctagtag aatacaccat tggggcttta
     3421 cgaaaaagaa gagaaacaaa cttgtcaata gtaaaaaatc tagtaaactc acataccatt
     3481 ttttcaacgt atagtagaac gctatccgta gaacttgatt tggtttcgac gctggatttc
     3541 tcacctgaaa ataccagtag ttgatgctaa aggaaactga taaacaagca agtaaaacaa
     3601 acaacggaat caaccatttt ccataagaaa aacgtataaa aaagaaacaa tcaaaaatca
     3661 ttatcacact aactatccaa gcaaatagcc aataaaaaaa tccctttagc caaaattggc
     3721 gaaacgtctt tatgaacaaa cgtactggtg aaaaactttt ttcttttgct aattcttctg
     3781 tcatacctat catcacgatg caacctggac caataaaaag taaagaaaaa ataaaggaaa
     3841 agacattcct tccatctatc gacaaaagga acgcagctgc tatatacaag atattgacac
     3901 accaaaaaca aaggctactt gccaatgatt gatacaacca ataaaagaat tccatataaa
     3961 catttttgtc aaaacgctgc tctttcataa tcacctccta ttccttcatt cctgatgaag
     4021 cgatcccttc tacaaaatat ttttgcatcg ctaaaaatat aaaagcaaca ggaagaacag
     4081 aaacaactga ggcagccatg attaatgcgt actctgcatc aaatagccca ataaacattt
     4141 tcaaccctaa ctggattgtt ttattttccg tagaagacaa ataaatgaat ggccccatat
     4201 aatcgttcca tgtattaacg aatgtgatga tcgtcaaact ggcaatcgcc ggttttgtaa
     4261 gaggtaaaat gatcttgcga tagattcccc actctgataa gccatcgatc cgcgcactct
     4321 cacataaaga atctgggata ctactgtagt attgcttcaa caagaacaca ccaaaagcat
     4381 tgaacgcttg taatagcacc aacgaccaca acgtatatgt taaacccact tttctcatca
     4441 taatgaactg cgggatcata taagactgcc aagggacagc aatcgtgccg atatatgcta
     4501 aaaataaaat atcgcgccct ttaaaattta gtttagaaaa cccgtaagca gcaaaactag
     4561 aagtaaacaa ctgaatcaac gtgatcacaa cgcttaaaaa agctgtattc ttaaaaaatg
     4621 tcactaaagg aatacgagac caaataacca gataattctc taaatgccaa gttttaggga
     4681 tccattgaat cggaatcgtg aacacttcat tatttgcttt gaatgaagca gaaaccatcc
     4741 ataaaaaagg aatgatcgtc aataaagcta atacaatcag cagaatcata cccaaccatt
     4801 gaataaaggg catgtttttt gaacgaattt ttgctttttt tctttcattt cgaacacctc
     4861 caaagttata atcttcattt ctttgtaatg attacaatcc agaacaacgg ttattatagc
     4921 tgattcattt ccgttcttct tatgtttgta agcgcttatt taacaagtta tgtaaatctc
     4981 tttatttttt ttgatttaaa aatgaaaaaa tacaaaagca agactgtcat acgtctcgct
     5041 tttgtattga aatttcttcg attagagata cttattactg ttcaaaagct tctttgaaac
     5101 ggtgaatcaa ttcattgact acaggtgata aaatccgatt ctttttccag actagcatgc
     5161 agttcgtttc aatcattggg tcaagcggaa cgaatgtcac tcgctttgaa agatgactta
     5221 cagcaccttc tatgactaaa gcagcaccta cctgattctc aattagcgca aatacattga
     5281 aaatcaaatt gtacgttcct gtgatcagca actgctcctt ttttatgcct aaccagtcag
     5341 caaacattcg ctgtatttct ggacgctttg agatcaaaag aggtacattc gcaaagtctt
     5401 cttttgttat cacgtctttt tttgctaaaa cagaattttc taaaatgagc aatccccatt
     5461 tttcctttct tggtaacaaa atcgtttcgt atttctctgt atctactgga tctagcaaaa
     5521 tcgctaaatc taacaggctt ttttctagtc actccgtgat atcatcactt gttccgctgt
     5581 ggatatgaaa ttgaacgctg ggataatcac ggatcaactt ttctagtaat agtgccaatg
     5641 tattcgaatt atcagcttcc acacagccga tcgtaaatcg gccgctgaat aattgttttt
     5701 ttctgttctc gaattcttga gtggtttgat tcgttaaatc taaaatttct tctgctcggc
     5761 ttttttagaa aataacctgc ttctgtcaag atcattttgc ggttttcgcg tacaaacagt
     5821 tctgttccta gttcctcttc taactccttc agttgtcggc ttaaggttgg ttgtgtaaga
     5881 tgtagaactt ctgcggcttg agagattgtt ccttcttctg caattgtcca aaaatatttt
     5941 agtaagcgta actccatagc gatgctcctt gattttttta cttgatacat tccattttat
     6001 ttctagataa cacacgatca tatttcttga tttttccttg taaacgttcc aaacattctt
     6061 gcatttcagc aatttttttc gtcaaaagtt cactttcttc aatcaaaagc ttacgccgct
     6121 caaatacagt tgcttctcct tcgctagata ggcgaatata ttcaatcaaa gactcgatcg
     6181 ataatccggc atttcgcata cagatcgtaa agtcgaccca ttttaggtct tcttctgtat
     6241 aatcacggat tccgcctttt gtccgattaa ctggtggaat caacccgatt cgttcataat
     6301 agcgcaatgt atcggccgaa atgccctttt cttcactgac ttgtttgata ttcatctgat
     6361 cactctttct cttaatttct tgccacttta cttatgtatt ttatttattc tacaacttta
     6421 agtgtactcc aagtcaatac tcatttctat taaaactaag ctggattatc tatatcggtt
     6481 gtttgtgtat tatttcaaat aaaaaagtct tgttttttta ttcttttttg gatatacttt
     6541 taacaattta gggaaatttt aggaaaaggg gtataaaaat gacaacgtat cagcgaaagt
     6601 tattattatc gacatcggcg ggaattgctt tagagaatat ggatattatg tttttagctt
     6661 tttcattatc ttcaatgatt acttcttttc atattagtgg tacgcaagcg ggtttcattt
     6721 caacgatcac taatttgggg atgctcattg gaggtatttt ttttggcatc atagctgata
     6781 aatatggacg tgtgaaagta ttttctcaaa cggtccttct gttttcgatt gcttctttat
     6841 tgatgtattt tgcttcaaac atctacttgg tttatctctt tcgctttatc gcaggtgtag
     6901 gagcgggagg agaatacggg gcgtgtatgt ccttgatttc cgaaagtttt tcaaaaaaac
     6961 aaattggacg agcttcttcg gttgctggta ttggtgcgca ggtcggtgcg gctttggctg
     7021 cgattttagc tgctgtgatc attccaacgt ttggttggaa gatgttgtat gtcgtaggtg
     7081 tactgccggt gttgatggtc ttgttcattc gtcgcggctt gactgaacca aaggaattca
     7141 aatctgtaca atctgccgaa aagaaaacca aacttagcag actgtttgaa tcaaaacatc
     7201 ttgcttggca aacatgtggc cttagtatca tggtaactgt ccaaattgcc ggctattttg
     7261 ggttaatgaa ttggctgcct tcaattatgc aaaatcgatt agggctttcg gtttctgggt
     7321 cttcattgtg gatggtcagt actattttag gcatgtcttt ggggatgatg acttttggca
     7381 taatcatgga taaattgggg cctaaactag ccttttcttt gtttttaatt tgttcatcct
     7441 tatctgtcta tctacttgtg ttagcccata gccagtggag tttggtagca gccgctgtga
     7501 tagtcggcta ctttatcaat ggcatgtatg gtggatatgg cgcagtgatt agtagtttgt
     7561 atccaacaga aatccgcgcg acggcgaata atttcattat gaaccttgga cgagctgttg
     7621 gtggtttttc ttctatcgtc atcggtttct tgatggatca ttatagttta actgccgtaa
     7681 tcttgttttt gagcggtatt tacctagtca gtctgctggt cttgacaact cttactggtg
     7741 ttacagcatt aagggaaagc ttgcagcatt gatagttgag tctgtaaatg acaagaaaga
     7801 ggtgtggcaa aagcttgcat ctgcggtaat aagaccagcc aaatcaaaaa catgagacac
     7861 tgtttccgtt tggctggtcg tattacttga tgcagggcag ctttttcaac tgctaatttc
     7921 cttctatctg gcggctacta ttctttgacg tccgtcattt caaagtactt tactaataac
     7981 ttagcataac gaaaaactgt ggatacactc gtatattgag cgtctgcata atcttccaaa
     8041 tttttctcag gatgttgata catgaattca atcaaatgat aattatatga ttttctgtag
     8101 aaatgaagat aaataatgta gattgggaag ttctcctgct tttccataga aaagcgccga
     8161 ttattctcat tcttcaaaat gaccttatca ggatattttt ggaacaattc ttctaaatct
     8221 gttagataat tcaagataga cttacttgat accttcaatt cgttttgtag ttcttctatt
     8281 gaacaccaac gttctgtttc aagcagtaaa tgaatcattt taattttctt ttgagaaact
     8341 tttgttaaaa aagtctgcat attttctcct cctgtatcta ctttactata ctacatacaa
     8401 gaaaaaaaac ggatacaaat atcatattgt cacaccgtca atagtgcata aacctgttat
     8461 tattt
//
LOCUS       DACECZ010000106         8396 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.107, whole genome shotgun sequence.
ACCESSION   DACECZ010000106 DACECZ010000000
VERSION     DACECZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.107"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            7..2199
                     /locus_tag="IWO35_002230"
     CDS             7..2199
                     /locus_tag="IWO35_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ0317272.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNTGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            2216..2692
                     /gene="ybeY"
                     /locus_tag="IWO35_002231"
     CDS             2216..2692
                     /gene="ybeY"
                     /locus_tag="IWO35_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ0317273.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            2670..3071
                     /locus_tag="IWO35_002232"
     CDS             2670..3071
                     /locus_tag="IWO35_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ0317274.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQIDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            3086..3985
                     /locus_tag="IWO35_002233"
     CDS             3086..3985
                     /locus_tag="IWO35_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ0317275.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYTALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            4128..4940
                     /gene="recO"
                     /locus_tag="IWO35_002234"
     CDS             4128..4940
                     /gene="recO"
                     /locus_tag="IWO35_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ0317276.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFLSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHAYPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKMPEKNMPEKEIPEKEE"
     gene            5324..6241
                     /gene="glyQ"
                     /locus_tag="IWO35_002235"
     CDS             5324..6241
                     /gene="glyQ"
                     /locus_tag="IWO35_002235"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0317277.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRF
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARSVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            6243..8318
                     /locus_tag="IWO35_002236"
     CDS             6243..8318
                     /locus_tag="IWO35_002236"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ0317278.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDIKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKDHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEV
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         2841 a         1514 c         1798 g         2243 t
ORIGIN      
        1 aagcacatgt tgaataaaac actgcagaaa ctacaagaaa aacttggaaa ggcagcattg
       61 cctttcctgc tgttcttgtt ttttatcatt atctgcggcg ttacatttag tagtgtcaaa
      121 caaaaggcca atgatttcag agaaggccaa gtagcagaag aaagtatccg tgcgaacaaa
      181 acgatcgaga acacggaaga aaccgaacaa aaaagaaaac tggcagcaga agcagtcact
      241 ccagaataca cttaccaaaa agatcttgca gatgaccaga acaatcggat caagcaacta
      301 tttgaattga tcgataagac gaatgatgcc atcaacaaat cttatgacga aaagattgat
      361 aaggcaaagg aagatgaaaa tatcccaaag cctactacag aagagcggat cgccgcgctg
      421 aagaaaaatt ttgaaaatgt gaatgcagaa aatgttgtgt tttatcaaaa actaccgaac
      481 aatttttatt cgacgatatt tgaaatgact gaaactgaga ttcatactgt gagggatgag
      541 agtctgaagc tcattgacga acagatgagc aaacaagttc gtgaaagtga attggaagct
      601 tttaaacaag aagcagaaga gcaaatccaa tatttgaatg tcacaccagc acaacaacaa
      661 atgattcgct acttagtgga tcaaggtatc gtagtgaatg acgtattgaa tgagaaaaag
      721 acagaagaat tgaaacaatc cgcacgagaa gctgtacaac cagtgatgat cttccaagga
      781 gaaatcattg ttcgtgaagg taatcaaatt gatgcttcag ctatgaaaaa attggaatta
      841 ctaggactga ctaatcaaac aacttcgatt tttccgctaa ttgccatggt tttagctgtt
      901 ttgcttcaaa tcggcgtact gatttataat tcacttcaat tccaaaatac aggagaaaga
      961 acaaaatacg ttcttttcta tgtgacagct atgtcagtca gcatagtgct gatgaaattt
     1021 ttccaattat tccaaacgga acaagctact tatatcccgc tgttttatcc cgctgctttt
     1081 gcaccacttg ttttaagctt tttcttgaat cgaagagcag ggatcatggc tggtattttc
     1141 caagcagtat ccgcactttt cattttttat gattcgattg gcacgaactt tctaacgatt
     1201 atcttgatgt cttatgtttt ctctgggctc atggcaacga ttgtcagacg taaacggatc
     1261 tcagaacaag gattatctgc tgccatgtgg gtcattattt ttcctgtgat gatggatgtc
     1321 attttgatcg tctatcaagg aatgagcttt ggtgatgcaa cgacttggtt aatgcttgtc
     1381 tgcgggttca ctggtgcact cttctcattc ttgctgacga tcggtctaca cccatatatt
     1441 gaattgatgg tcaatgatga cagtgtaatt gtattaaatg aactcagcaa tccaaatcat
     1501 cctttattga aacagttgct ggaagaagcg ccaggtacgt atcatcatag tatgatggta
     1561 gctaatctaa gtgctaacgc tgtagcagaa atcggcggac gttctttact aactcgagta
     1621 gcttgctatt atcatgatat cggcaagatc aaacatgcta gctttttcgt tgagaacttg
     1681 ccttcaggag cggaaaaccc gcacaatttc ttattgccag aagacagtaa acatattatc
     1741 tttggacatg tgacagatgg agccaaaata ttggaagatt ataatatgcc gcaaatggtg
     1801 atcgatattt gcaaacagca tcatggaacc acgctaatgc gttatttcta catcaaagcg
     1861 aaagaacgca atccggaagt aacagaagag caattccgtt atcctggacc aaaaccgcaa
     1921 agtcgtgaag cagggatcgt caatattgct gacagttgtg aggctgcagt acgggcaatg
     1981 gatcatccaa cgagcgaaaa aatcgaaagt tttgtccata atcttatcga agagcgtatt
     2041 tccgatggtc aattagacga tagtggattg accttgaaag aaatccgaaa agtggaaaaa
     2101 tcattgatca gcggtttaag tagcaccttc cattctcgga tcaaatatcc taaaatgaaa
     2161 acagaagttg agaagataaa ggaagaacaa gaagaataga aagaagcgat gtataatgga
     2221 tattaccttt atggatgaaa caaaccaagt ctcagaagag aaaatgaaag aaatcgatga
     2281 cttgctgcaa tttgcagcat cttatttaaa gctgccagag gaaacagaaa tgtctgttac
     2341 tttcatggac aatgcggcaa tccaagtaat caatcgtgat taccgtggaa aagatgcacc
     2401 gacagacgtc atcagttttg ctctggaaga agaaggcgaa gacgaaatcc cagtgatttt
     2461 tgaagatgga gagaatccat tgcctcgtgt actaggagac atcatgatct ctattgaacg
     2521 tgctgctgaa caagcagaag cttacggcca ttcatatgat cgggaacttg gctttttagc
     2581 ggtacatgga ttcttgcata tcaacgggta tgaccatatg acaccagaag atgagaaaga
     2641 aatgtttggt ttacagaaag agatcttgga tgcctatgga ctcaaaagat aaaaacgtag
     2701 agaaaaacaa acatttcctg atgtccgtgg aatttgctgt ccaaggagtc aaaaccgttt
     2761 ttgaggaaga acgcaatatg aggaaacatg ttgcgttcgg tatccttgct ttatttatgg
     2821 gcgtgatttt ccagattgat cggatagagt ggttatggct gcttttagct gtttttctcg
     2881 tttggatagt agagatcatc aatactgttt ttgaaaatgt agtggacatg tttacggatt
     2941 ttcattttca tccgattggg aaaaaaatca aagatatggc agctggagca gttttagtga
     3001 ccgcttgttt tgctgtcatc attgggttga ttttatttgt ccctaaaata tatcatttgt
     3061 tgtttaatta aaaaggagaa cgtatatgcc agaacataaa tcagggtttg tcgcaatcgt
     3121 cggccgcccg aatgtaggaa aatccacatt attgaaccga atcgtgggtc aaaaaattgc
     3181 cattatgagt gacaaggcac agacaactag aaataaaatc caaggagtat atactacacc
     3241 agaagctcag ctgattttta tcgatacacc aggaatccat aaaccaaagc atcgtctagg
     3301 agattttatg gtagaagcag cttacactgc gttgaaagaa gtggatgcag tcttatttat
     3361 gatcagtgct gatcaaaaac gcggccgggg agacgatttt attattgaac gtctaaaaaa
     3421 cgtgcattct ccagtttatc tgattatcaa taaaatcgat aagatccatc cagacgagtt
     3481 gctgggaatc atcgaagatt attcgacgca gatgcctttt gcacaagtga ttccgatctc
     3541 tgctacagaa ggaaacaatg tcgaacgctt aatggatgta ttagtcagtg aaatgccaga
     3601 gggcccacaa tattttccag atgatcaagt gactgatcat cctgaatact tcatcgtttc
     3661 tgaattgatc cgtgagaaag tcttgttttt aacacgagac gaagtgcctc attctgtcgc
     3721 agtcgttgtt gattcgatga agcgaaacga gaatgataaa atccatatcc aggccacgat
     3781 catcgttgaa agagatagcc aaaaaggaat cattatcggc aaaggtggaa aaatgctgaa
     3841 gcaagtagga acgaaagcaa gaaaggatat cgaaaatctt ttaggagaca aagtatttct
     3901 agaactttgg gtcaaagtcc aaaaagactg gcgagacaaa aaagtctatc tgcaagactt
     3961 cggctaccga aaagacgagt actgatattc tggacttctt ataggttagg tatagaaata
     4021 aactggataa aaatccgtac gttgagaaaa acagcgaaag atcgtaacat aaataatctg
     4081 gatcggcaga atctgctttt ccagatttat tttttaggag ggagaatatg agccagcttg
     4141 cagaatcgaa aggcttgatt ttatctagcc gtaattacaa agaaaaagat aaattagtca
     4201 aaatctttac ggaatcttct ggcaaaatga tgttttatat caaaggaatc catcggcaaa
     4261 atcagccatt agctccggct ttgctcccct ttaccgaagc agtctatatc ggtaattttc
     4321 taagtgaagg tttgtcgttt ttaagcagtg tcaaagacgt ccatgcgttt cggaacatcc
     4381 aagaagatat ctttttgagc ggttatggta cgtatttgct aaatctagta gatgcagcaa
     4441 tcgaagatca tcaatatgac ccgcacttat tccaatttac gcaacaagct ctgcagcgga
     4501 tggaccaagg agatgatgca gaaatcatca cgaatatctt tgaagttcaa atactgcaac
     4561 gcttcggaat cgcgccaatt tggacgcact gtgtcgtatg cggggaaaca aaaggaaaat
     4621 ttgactattc ttccaaatat ggcggcgtga tatgcgagaa acactggcca atggacgaac
     4681 accgttatca tgcatatcct cgcgctgttt atttcgttcg aatgttttcg gctatttctt
     4741 atgataagat cagtggaatc cacttgaaag aagaaacgaa gcaggcaatc cgtgacctca
     4801 ttgaccagtt gtatgatgaa tatgtcggta ttcatctaaa aagcaaaaag ttcattgatc
     4861 aaatgaagtc ttgggcacac gtattaaaga tgccagagaa aaatatgcct gaaaaagaga
     4921 tacctgaaaa agaagaataa atgaaagata caacaaaaaa atcaaaacta tttgacaaat
     4981 acagggactt ccgtctatta ttaaccgtag attaaataga tcgttcactg agaggatcta
     5041 ttatataaac aagaaaactg caaaatgaat acgtacattg accaagatca gtaaaaaaca
     5101 cgtggtttag cgagttcggg agagtggaag ccggacccaa acgatttttg aagaggcact
     5161 tgcgagtacg ataaatgaag ctgccgaatc tttattcgga agtagggtgg aaccgcgaaa
     5221 ttattcgtcc ctatgtctgt aatcagacat agggactttt tgtcgttttc tttcctgtat
     5281 tcttattgac agcaacgctt aaaaattgaa aggggcaaaa cagatgagta aaaaattgac
     5341 cgtccaagat atgattttaa cattacaaaa attttggtca tcaaacggct gcatgctgat
     5401 gcaagcttat gatacagaaa aaggagcagg tacgatgagc ccatatacat tcttacgggc
     5461 aatcggaccg gaaccatgga atgctgctta tgtagagcca tcccgccgac cagctgacgg
     5521 gcgttatgga gaaaacccaa accgtttgta tcagcatcat caatttcaag tcgtaatgaa
     5581 accttcacct gaaaatatcc aagagcttta tttagaaagc ttgcgttttt taggaattga
     5641 tccattagaa cacgatattc gttttgttga agacaactgg gagaacccat cgatgggttg
     5701 tgccggtgtg ggttgggaag tatggcttga tgggatggag attacacaat tcacgtactt
     5761 ccagcaagtc ggaggactac cttgtaaacc agttacttct gagattactt atggactaga
     5821 acgtcttgct tcatatattc aagaagttga aagtgtctat gacttagaat gggctgatgg
     5881 cgtgaaatat ggcgagatct tcaaacaacc agaatatgaa cattccaaat attcttttga
     5941 agtaagcgac caagacttgc tactaagcaa ctttgatcgt tttgaaaaag aagctaaacg
     6001 ctgtatcgat gaaaatcttg ttcatccagc gtatgattat attttgaaat gcagccatac
     6061 attcaattta ttagatgccc gcggtgcagt atccgtaacg gagcgtgcag gttatctttc
     6121 ccgtatccgc aacatggctc gttctgtagc caaaatcttt gtggcagaac gcgaaaaatt
     6181 aggattccct ttattgaata aaaacacagc aaatccagaa aacgtgaagg aggctgaata
     6241 agatggcaaa agacttatta cttgaaattg ggctggaaga aatgcctgct catgttgtca
     6301 caccaagcat gaaacaatta gaagaaaaaa cagcaaaatt tttagatgaa aaccaattga
     6361 cttatgatac gattgaaaca ttttctacac cgcgccgttt agcaatcaaa gtaacaaaca
     6421 ttccagaacg tcaagcagac agtgaagaag aagtcaaagg accagccaaa aaaattgctt
     6481 tggacgcaga tggaaactgg acgaaagcag cggaaggatt tgtccgcgga caaggtctga
     6541 cgacagatgc tatcactttt agagaattaa aaggcgtgga atatgtctat gtaacgaaat
     6601 acactcacgg caaagaagca aaagaagtat tgacaggttt agcagatgtc attaaaagcc
     6661 tcactttccc agtaacgatg cattgggcag aatacgattt tgaatatatc cgtcctattc
     6721 actggatcgt ggcattgtta gatgacgaag taatcccgtt tgagatcttg gatatcaaaa
     6781 cagacagagc ttcaagaggt catcgtttct taggcgaaga tgtgacattt gcgcatgcaa
     6841 aagaatacga agaaaaatta aatgaacaat ttgttattgc ttcaccgcaa aaaagaaaag
     6901 aaatgatcat cacacaagca aacgaattag cagaaaaaga tcactggatc attgatctag
     6961 atgaagattt actagaagaa gtcaacaatc tagtcgaata tccaactgtc ttttctggcg
     7021 acttcgaaga aaaatatcta tctgttcctg aagaagtact agtcacttct atgaaagaac
     7081 atcaacgtta tttcgaagta cggaacgatc aaggtatgtt gcttcctcat tttatttctg
     7141 ctcgtaatgg cgatcgggta aaattggaaa atgtggtacg aggcaatcaa aaagtattga
     7201 ctgctcgttt agaagatgcg gaattctttt ataatgaaga caagaaagct tcaatcgacc
     7261 aatacgttga aaaattaaaa actgttactt tccatgaaaa aatcggtagc atctatgaga
     7321 aaatgcaacg ggtacaagtc atcgctcgca tcataggcga acaagtagga ctgtctgagg
     7381 aagaattgat ggatctgcaa cgcgctgctg aaatctataa atttgatcta gtaaccaaca
     7441 tggtaggaga attccctgaa ctacaaggga tcatgggaga aaaatatgct ctgcttaaag
     7501 gcgagaaacc agccgtagca acagcgatcc gtgaacatta catgcctata tccagtgaag
     7561 gagatttacc tgagactaca gtaggttctg ttttagcgat tgctgataaa ttagatagta
     7621 tcttttcttt ctttgcagtt gggatgatcc caagtggctc aaacgatcca tatgctttgc
     7681 gccgtcaagc atatggtgtc gttcgaatca tcgaatcgaa aaaatgggcc tttcctttgt
     7741 ctgttctgca agaaacgatt agtgaagtta tcagtaaaga tacggatcgt tttggcattg
     7801 gcttgtcagc gggacaacaa caggtgatcg actttatcaa aggccgtctg cgccaattat
     7861 tgacaacgaa gaatatccgt catgatgtca tcgaagctgt tttgaacgca gaacaaaaag
     7921 atctgacaaa agtatttgca gcagcacaat tgttcaagca gcatcttgca gatgaagact
     7981 tcaaaccatc gatggaagct ttgacacgtg tcgtgaactt agcgaaaaaa gcggagcttg
     8041 aaaaacaatc agaagtagac cctgaactgt tcgaaaacga agcggagaaa gaactgcaca
     8101 aagcagtaga ggtaatgaaa acaagctttg ctgaccaaac gatcaacgaa aattatgatc
     8161 agcttgtaag ccttcgccca ctgatcgaaa attattttga tcagacgatg gtcatggcag
     8221 aagaaccagc agtacgtaag aaccgattaa cgcaattaag tcaaattgct gccatggctt
     8281 tgtcactagc aagtttagat caaatcatca caaaataatt tctaatatgt aaataaacca
     8341 gagaacaggc acaaaatagg ctatttctct ggtttatttg ttataaatat aaataa
//
LOCUS       DACECZ010000107         8250 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.108, whole genome shotgun sequence.
ACCESSION   DACECZ010000107 DACECZ010000000
VERSION     DACECZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.108"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..361)
                     /locus_tag="IWO35_002237"
     CDS             complement(<1..361)
                     /locus_tag="IWO35_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ0317279.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSL"
     gene            complement(448..1152)
                     /locus_tag="IWO35_002238"
     CDS             complement(448..1152)
                     /locus_tag="IWO35_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAQ0317280.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            1352..1618
                     /locus_tag="IWO35_002239"
     CDS             1352..1618
                     /locus_tag="IWO35_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0317281.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            complement(1786..2016)
                     /locus_tag="IWO35_002240"
     CDS             complement(1786..2016)
                     /locus_tag="IWO35_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0317282.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            2088..2243
                     /locus_tag="IWO35_002241"
     CDS             2088..2243
                     /locus_tag="IWO35_002241"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317283.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            complement(2552..2776)
                     /locus_tag="IWO35_002242"
     CDS             complement(2552..2776)
                     /locus_tag="IWO35_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0317284.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            3043..3378
                     /locus_tag="IWO35_002243"
     CDS             3043..3378
                     /locus_tag="IWO35_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317285.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3611..4552
                     /locus_tag="IWO35_002244"
     CDS             3611..4552
                     /locus_tag="IWO35_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ0317286.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            4554..5444
                     /locus_tag="IWO35_002245"
     CDS             4554..5444
                     /locus_tag="IWO35_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAQ0317287.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5487..6287
                     /locus_tag="IWO35_002246"
     CDS             5487..6287
                     /locus_tag="IWO35_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0317288.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6302..7153
                     /locus_tag="IWO35_002247"
     CDS             6302..7153
                     /locus_tag="IWO35_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0317289.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7150..7467
                     /locus_tag="IWO35_002248"
     CDS             7150..7467
                     /locus_tag="IWO35_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317290.1"
                     /translation="MNLTKQEKRICRQIMLQIIGDRDMDELSQQESRMVRKISKLIGA
                     DTMDRSKPLPKLNFESFTFEKYSHLREVGYSVNAIRKALGVGSSVWAKWRKENVNEVN
                     QND"
     gene            7460..7642
                     /locus_tag="IWO35_002249"
     CDS             7460..7642
                     /locus_tag="IWO35_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317291.1"
                     /translation="MIKLIGKETLKVYYVAETKAEVSRWLLKMFNKTRANASVSQKAS
                     VSETGMPEAMWISRRN"
     gene            7650..8069
                     /locus_tag="IWO35_002250"
     CDS             7650..8069
                     /locus_tag="IWO35_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAQ0317292.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            8066..>8250
                     /locus_tag="IWO35_002251"
     CDS             8066..>8250
                     /locus_tag="IWO35_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317293.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDP"
BASE COUNT         2934 a         1349 c         1686 g         2281 t
ORIGIN      
        1 ataaagaaag tttgtttttt atttcggatg aagaagtttc atctatattc ggaataatta
       61 ctgatttata gatagtgacc agagatgtgt catatttttg attaatttca tcgtctaacc
      121 gctggctttc agcattaaga atatcaattt tgttttctag cgttgacaag tttttattca
      181 atttacttat gtttttctct ttttcagata taaacgtttc cctagcattt aattcttgag
      241 aatattcctt taccttactc tctaaggcag aagttttatc ttttaatttt ttagcttcag
      301 ataatttgct actacccata attattaaaa ctattccaac taaaaacaat agtactccca
      361 ttcaaaacgc ctcgcttctt ttataaaata attgtcaaaa tcatataaac ccttaccgaa
      421 gttgacggct ccggcatggg aattttttta aaatacaact tttccgataa ttctaatctg
      481 ttctggcgat acaattctat cttcgtataa gtcgtttata gaacgtaaaa tgattgtttg
      541 attttcgtaa tctttatata tctctttaca tgtaactccg tcaccaccta attccacaat
      601 agcaatttcc ccatcttcta cgtccggttg ttctttaacg aatacgaatt gtccattcct
      661 gataacaggt tccattgaat cgcctataat aggtactgcg aagtcagcag cacttggaac
      721 tctctcaaat tcttcctctt cgacaaccgt atcaccgtaa gtaagttctg ttggattggc
      781 agccgaagcc gaattgtgaa cagttgggat tttttctttt ttaggaaaat ctaggacatt
      841 ttcttgtttt tgttcttcta atcgattact tgcaaattca taggtagcaa ctttattttc
      901 gtcgttaagc tgattatata taggaaggat attatatatc tgattagata tagataaatc
      961 aatattcatc aaatcatcta tatcaacatt gaatatctta gctatctcat ttaaggtttt
     1021 taattttggt gtatattttc ctttttccca ttcgcttatt gaggatgaac tttttcttcc
     1081 tagtttttcg gctagttctt gttgatcaat attatacttt tctctaagaa actttagatt
     1141 ttcagcgaac atagtaacac ctccttgagt taattgtaac accgtttcgg aaaaaatgta
     1201 acaggtttct tagaaaatat tttagtgttt ttaaagaagt atttacgcaa aatagaaatc
     1261 gatttcagga aaactgaaat atgcacttga cttcggaaaa accgaagtat ataatttagt
     1321 cagaaagtta aagagaggag gcgaataaaa tatggatacc gcgaagaaag aaaaatatac
     1381 attgagacaa ttaagagctt taaagggtta ttcaaaagaa gaactgtcaa ggaaaagcaa
     1441 agtgacatca agaacaatat ttatttacga aaacgatgta gataaaatga ggaacggaaa
     1501 gtacgccact ctagaaaaaa ttgctaaggc attgggtgtt agagtttccg atattttttt
     1561 agaccctgat tcggaaaaac cgaaatcaat aacaggtgaa acaaaacaag tcagctagga
     1621 ggtgtgaata gtggaagaga agaatataaa agtaccgact cttgaagaat atacaaaaat
     1681 tgttgtcgaa acagacgagc agaacccgaa aacaatcgca gttattacgg cgaatgatat
     1741 tgaatcatgc gaaggttttc gagttcgaat gacccctaga tatgattaat gttctttatc
     1801 acgtggaggt ataggatcgt ttccaaaact atcttttgat tgtatttgtc cattcttttt
     1861 ttggacaata aactctgatt gctgtttttt agaaatcttt ttaccatact caacagcatc
     1921 tgatttatta tcaaatagtt ttgtatcttc ggaattacct tcaccacgta cagcccattt
     1981 gccatttcta ggagataccc attgattttt acccataaca attcacctcg ctttcaagtt
     2041 aattctatta agaggtgaaa agaaaacaaa ctgatggagg tgagattgtg agtaacaagc
     2101 caaaaatcac tttgaatggc acacctaatc ttcaaagatt agcagatgaa atggctagaa
     2161 tacgttctag acattgggga gttactgtgg tggcaaccat aattgaacaa aaagaaaagc
     2221 ccacacagcc ggcaagcaag tagtgggcac catagaacaa taatattata tcacacactc
     2281 gccacttccg aaaagaggag ttgctgctct cctttcaagt ttgccaaagt ccacgtttct
     2341 tcattttggc aactcctctt cttggaggtg gtgacggcga gagacgaagg agggatgaca
     2401 tgaaggaaga atcaaaaata gggattttga atgagaacaa ttacacaaga ttaattattg
     2461 aaacagaaga aggggaaaaa gtagcagaag ttacattgac cgatgcaact cctgctgacg
     2521 gttatcgtat tcgattaact cctaaatatg attaaccttt tggtgggtgt ggatcgtttc
     2581 cgtgactatc acggctttga atttttccat ctctgccatg aataaatagc tcagatcctt
     2641 gatttttaga aatctctcta gcgattttta ctgcttctga ttttgtatta gtatgtgcag
     2701 tcgctttaga atttccagca cctttgacat tccaaccacc attggaatta ggtacaacat
     2761 gttgattttt tcccatagtt ttcacctcca tttctataga aatattttat caaatagaaa
     2821 tggagaaatt caataaacga aaggagggat caaatgacat tacaagagca tcaacacttg
     2881 atgcgaaaac taaatcagga ataccacgag gaaatgaaac ctagattgct aggggaacaa
     2941 tggcgtgaac gccagaagaa gtggttagaa ataaaaaagt gactcagccg tccaaagcaa
     3001 tgagtcacaa accaaaaatt atacctaagg agattttatc acatgaataa gaaaattgaa
     3061 aatcttatcg aagaattgaa acgtgaatgc caaaagcaag gtgtttctat tatttgtaca
     3121 gcccaaaaag aaggggaact taaaagtctc gtttatggtg agacaacaga aattttactt
     3181 tgtcttgcta tgcaagaaga acatttagat gaaaatctcc cattgtcagc acatattatg
     3241 cgtagaattg ctgtggatgc ttacgaacaa gcgaaaaatg aagaagaaaa tcaaccttca
     3301 aatcatactt ttgttattaa taacaaggaa gatttagctg atgtgatgac tcgtattctc
     3361 aagggggaat ttcaatgatg aatgaaaaaa caattgatct actcattatt ttatttttca
     3421 cgttagtgat tgtagcgaaa agttatgtaa tctttggctt gttattcgca attaccatgc
     3481 ttgtgctttg cgtgttagca agcagaaaag aggttaaaca tgaacgagtt agcaaatctt
     3541 gacaactatt taactgatcc tgattatgct aaaccaccat atgaagcgcc aattgatgag
     3601 gaggatgaag atgagtaaat ctaccttaga aatgagccat caagaatggc tcgaagaccg
     3661 caaaagaggt attggtggct cagatgttgc aaccgtactt ggattaaaca aatacaaatc
     3721 tccttatcaa ttgtggcttg agaaaacggg tcaaattgaa ttgaaagatt tagaaagcga
     3781 accagcttat tggggtaatg ttttagaaga agtggttgct aaagagtttc aggaacgtac
     3841 aggcaaaaaa gtacgcagaa gaaaccaagt atttgaacat ccattacatc catttttaag
     3901 agcaaatatt gatcgggaag tagtgggaga aaatgccatt ctggaatgta aaacagccaa
     3961 tcaatttctc ggaaaagagt gggaaggtga agaggtaccg cttagctatc tctgtcaagt
     4021 tcagcattac atgaacgttt taaacaaaga ctattgttat atcgctgttt tgatcggtgg
     4081 acaaaaattc atttggaagc ggattgaacg agatcaagag ctgatcgata caatcactga
     4141 acaattagta gagttttggg aaacgaacgt tcttggaggt atcgagcctg taattgacgg
     4201 aagtggagcg actgctgact tcttaaaaga aaaatatgca gatgtagaag aaaatcaaac
     4261 agctctacca tcacgttttg atgaacttat cgagcaaaaa agagaactca agcggacgaa
     4321 aaaagaaatt gaatcatcta ttcgtcaagt agacaacgag atcatcagtg agctaggtaa
     4381 acgtgaagcc agtatcggta tcacacaaag aaacattatc agctggaaac ttgttcgtac
     4441 aagacggatg aacacgaaga aactagcaga gaaatatcca gatgtcgcaa atgatgaaga
     4501 gatttataac gttactgaat caagaaggct aaccgaaaag gagatcaaat aatatggcaa
     4561 caaatgaatc gttaaaaaat caattggcag aaaagccaca gaaacaagtt gcaccaggac
     4621 agttagggct taaagctcta atgaatacac caacaatgag aaagaaattt gaagaagtac
     4681 ttcatgacaa tgctaatgct tttatgtcga atgttatgac tcttgtatct aatgacagtt
     4741 atcttgcaga tagtgaaccg atgtctatca tgagtggtgc gttaactgct gcaacattaa
     4801 atcttgggct agataagaat ttaggttatg catatttagt tccattcaat agtaaaaaca
     4861 agcaaacagg aaaatgggaa aagaaagctc aattcatgct tggctataaa ggatatatcc
     4921 aattagccca acgatcaggt aaatacaaag cattaaatgt gatcgaagtt tacgaaggag
     4981 aactaaaaag ctggaaccga ctgacagaag agtttgagtt tgatccaaat ggtagaacgt
     5041 ctgatgaagt cattggatat gttggctatt tcgagttact gaatggattc aaaaaaactg
     5101 tctattggac caaacaagaa atcgaagctc atcggattgc taacaataaa gatcgcgata
     5161 agacaaagtt aagtggtgtg tgggcatctg attacaatgc aatggcacga aaaactgttt
     5221 tgagaaatct tctttctaaa tggggaatct tgtccatcga aatgcaagaa gccaccacat
     5281 cggatgagag agtccaaagg gttcaagaag acggcagcat tattgctgaa acagaagttg
     5341 aagaagatat tcctgaaaga aaagaagcag aggttatatc tgaagaaaat gaagatgtac
     5401 aaactggatt atttgatgca tctaatccgc cgttaaacaa ataatgaggg agctttctcc
     5461 cttgcttttc tagaggagaa atacgaatga acacaggata tgtcaaatta catcgaaaag
     5521 tgatgaattc attcgtttgg accaatcctt atatgtataa attatggagc ttgtgtttga
     5581 tgaaagcgag tcatgagaat cgcaagatac tttttaatgg aaaagagata caagtgaaca
     5641 gcggagaatt cgtcacaggg cgcgatgcta tcacatctga gatgaacgaa ggcgtgaaac
     5701 gtgaacatca agtgaacagc ggttctgtat ggagatggct aaaacaattt gaaaaaaacg
     5761 gaatgttgaa catcaaatca actacgaaat acagcgttat atcaataaaa aactggtctt
     5821 tgtaccaaag cagtgaacaa caaatgaaca tcaaacgcac aacaagtgaa caacaagtgc
     5881 acacaaacaa gaatgaaaag aattataaga atgaaaagaa taataaaaca tcatcgtcgc
     5941 aaccacgcaa aaagcgtgtt tacgacaccg actcagttta ctacattctc gcggaggagt
     6001 tattcaaaca gatttgtcag aatcaggaaa tcaaaaagcc gaatctgcaa agttgggcag
     6061 ataacattcg gaaaatgatc gagatcgaca agcggaccga aaaccaggta cggggaatga
     6121 ttgaatggag tcagcacaat gtgttttggg catcgaatat tttatctgct aaaaaattgc
     6181 gagaaaaata cgacacaatg gcagcgcaag caaatcgtga ttataaaaca aaacaaacta
     6241 aaacgcttga atacgagaaa tttagcacag atgagttgcc tatttgagag gaggcgtagg
     6301 catggaaact gttggggaaa tcatggaaaa gctgatacag aaagtgcttg tccaacgtgg
     6361 cgaatgtcct gaatgcggac agcctttgta tggatggcgt acgaagaacc ctgatggctc
     6421 agaacgttgt aagccaacct gtatgcaatg tgggtataaa gcattgcgag ttcaggaaga
     6481 cttacaaaca gaacgaattt acaacgagag cctgaaagca agagcaatca attttttcaa
     6541 aggtggttct gttgtgccta atcaagcgtt gtttgattgc acattgcaga attatcaaat
     6601 tgtcgatcaa gaaacaagac aagcggttga agtaaccaaa cgctttgtta attcagtctt
     6661 gttaggaaat ccaagtcacc ttgttttaac tggaaaacaa ggaacaggta aaagtcacct
     6721 agcgatggca gcggcttggg aagtcttgaa gcgatcaaac tacgataaaa aaatcttatt
     6781 tatcgggtta caggaaatgc tggatcaaat caaattttcg tacaacaatc ctgaactcag
     6841 aaaaacgatt gagggatcgt tgattgcaga tatcaaaaca gcggatttag tcatcattga
     6901 tgatatcggt tcagaactag gaaaagatgc atcagatagt cgagcgtttg gcataaacac
     6961 gctaaattcg ttcttggatg cacgacagaa cctagcaacg attatcacaa cgaacttgct
     7021 tggtgaagaa ctgaaaaaag cttacggtac gagaacaata tcaagaatgt ttgtcaactc
     7081 tgatggattt acgatggtat tttctcaaac agcagacaag cgcataaaac cagtcaaagg
     7141 agcgatcgca tgaatttgac taaacaagaa aaaagaatct gtcgtcagat aatgttgcaa
     7201 attataggtg atcgtgacat ggacgaatta tctcaacaag agagtcgaat ggttcggaag
     7261 ataagtaagc taatcggtgc agatacgatg gatcgatcca agccattacc caaactgaat
     7321 tttgaatcat ttacgtttga gaaatatagc catctgagag aggtaggcta tagtgtaaat
     7381 gctattcgaa aagcacttgg ggtaggaagt agtgtatggg caaaatggcg gaaagaaaat
     7441 gtaaatgagg taaatcaaaa tgattaagct aataggcaaa gagacgctca aggtttacta
     7501 tgtagcggaa acaaaggcag aagtcagccg ttggctattg aaaatgttta ataagactag
     7561 agccaatgct tcagtaagtc agaaagcaag tgtatcagaa acaggcatgc ctgaagcaat
     7621 gtggatttca aggaggaact gagatggcaa tgataccgaa gtttagagcg tgggataaac
     7681 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     7741 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     7801 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     7861 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     7921 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     7981 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     8041 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     8101 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     8161 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     8221 cggttggctt gagaaatcta cggacccttt
//
LOCUS       DACECZ010000108         8127 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.109, whole genome shotgun sequence.
ACCESSION   DACECZ010000108 DACECZ010000000
VERSION     DACECZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.109"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(341..1240)
                     /locus_tag="IWO35_002252"
     CDS             complement(341..1240)
                     /locus_tag="IWO35_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ0317294.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
     gene            complement(1261..1533)
                     /locus_tag="IWO35_002253"
     CDS             complement(1261..1533)
                     /locus_tag="IWO35_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HAQ0317295.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            complement(1556..2962)
                     /gene="lacG"
                     /locus_tag="IWO35_002254"
     CDS             complement(1556..2962)
                     /gene="lacG"
                     /locus_tag="IWO35_002254"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ0317296.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            complement(3104..4810)
                     /locus_tag="IWO35_002255"
     CDS             complement(3104..4810)
                     /locus_tag="IWO35_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0317297.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            complement(4810..5127)
                     /locus_tag="IWO35_002256"
     CDS             complement(4810..5127)
                     /locus_tag="IWO35_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0317298.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            complement(5155..6135)
                     /gene="lacD"
                     /locus_tag="IWO35_002257"
     CDS             complement(5155..6135)
                     /gene="lacD"
                     /locus_tag="IWO35_002257"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0317299.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            complement(6138..7070)
                     /gene="lacC"
                     /locus_tag="IWO35_002258"
     CDS             complement(6138..7070)
                     /gene="lacC"
                     /locus_tag="IWO35_002258"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAQ0317300.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            complement(7081..7596)
                     /gene="lacB"
                     /locus_tag="IWO35_002259"
     CDS             complement(7081..7596)
                     /gene="lacB"
                     /locus_tag="IWO35_002259"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAQ0317301.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            complement(7613..8038)
                     /gene="lacA"
                     /locus_tag="IWO35_002260"
     CDS             complement(7613..8038)
                     /gene="lacA"
                     /locus_tag="IWO35_002260"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAQ0317302.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
BASE COUNT         2488 a         1688 c         1402 g         2549 t
ORIGIN      
        1 cgctatattt aagatttcaa acaactatat taataggaag aaaatttcat ttttcctctt
       61 agttctataa aaagtgtggc taatttgagg caaaatatta aattaaattt gccacaaatt
      121 aatggctcca ctattatttt aaatcaagaa gtagtaaata atcttatttt ttactaccag
      181 actgtcttta gatattagct tttcgtcacc cactacagtt gacatagacc cacaaaaaac
      241 ttaaaaacct tgcgatgtaa gactggcacg gagaagacca atctatcatc acaaggtaag
      301 agtattcaaa tcttattgaa gactcaaata ataacaaatt ttaattaagg atagtaatat
      361 catagatttt tctagaagtt tcttgaggtg aaattttcgt tacctgtctt ttatcttcta
      421 agaaattacc ttcttcaagt gaagtggaga gaccactcca aggttccaaa gcaatgaatg
      481 gacttttatt agttgtagac cacagaatca agttcggaaa atcatcaaaa tctactcgaa
      541 gacctttacc agacttcctt gaccgaagcg ttacgctacg agacttgagc tggtcaagtg
      601 taatggcatc atgggaaaaa agtgagtagt ccaaatcgag aattttttga ttttccaaaa
      661 aaggtgtccg atcttgcaaa tctaataatc ctgtctcagg aaaactcttt ggtatcgagc
      721 aagactcaac ctcactaaat tcaatataat aatcctcata gttttcacct tctactaaag
      781 gactattgaa tgctggatgg gctccaataa agtaaggcat tgttttttcc atttccaagt
      841 tcgttacctg aaattcggtt ctaattgatt taccattcaa ggtataaaca actctcagtt
      901 caaactgata taggtaatta tcaagcatct cagcatttgg tttaatacta aaggtcacgc
      961 tattatcatt gacttcttcc agaataaatt cttcttttcg aacaaaacca tgtctacgaa
     1021 taagacctgt aaaaaagggt ctttcttgag gtctataaat agcccaatca tttcgtaaac
     1081 taccacagat aggaaataga ataggcgcct gtccattcca ataatttgga tcagcttgcc
     1141 agagatactc tatcccatcc ttgtctttaa tcgaagtcag ttgaccacca agtgttttaa
     1201 actgaacagt aagatactcg ttttttaatt cgatagtcac aactaattcc tttcttttta
     1261 ttattggaaa ggcaaataat ttacctcgaa gtgaatagtc tcacgtattt ctacatcgag
     1321 tgattgtaaa aattcccatt caaatgctga taactctaaa tcagaatggc aaatccactc
     1381 ttttccagtt gaaacatacc atttaccaca ttttagcaac ggggtagctt caagctcagg
     1441 aatcatagga caatcttcga aactaacaat atataccttt tcaaagcctg gtctaaataa
     1501 gtgttgtact ttttctaaag tatcggataa caatttttta cattttcaag taatattact
     1561 ctatcacttg agtttctgct actttcttat accaatgagc tgatttctta ggatagcgtt
     1621 cttgcgtgtc aaagtctaca tagaatagtc cataacgttt ttcgtatccg tttgaccatg
     1681 agaagacatc cataagtgac cagatgaagt aacctttaac atttgcacca tccgcaattg
     1741 cgtctgataa aacttccaag tgtttcttca cgtaatcaat acgaccatca tcataaacgg
     1801 tattatctac aaactcatct ttgtatccaa gaccgttttc agtgatgtaa atcttcttgt
     1861 agtttggata atcatttttc actcgcatga tttggtcata caagccttca ggataaataa
     1921 tccagtccca atccgttcta ggcacataat ctggtgcgat tcggcgacca acacctttaa
     1981 tctggtactt agaacttcct ttttcaccct taccattgtg aatgatttca gtctcaccat
     2041 caaaggcttg catccaatca ctcatgtagt agttgatacc aaggaaatcg ttcaaatctt
     2101 tagctgcttc aagtgcttgg aagtcttcat cacgaagatc aagttctcca ccattttcag
     2161 ctaggatgtg gttgacccct tccatcgttt tatctgaata gtgtccaagg taggttgcat
     2221 ccaagataaa tttgttatgg atgatatctt ctaactcagc ggcacgaaca tctgctggat
     2281 tttctggatc ataagggtat ttggttggca acgcatgtac tacaccaatt tcgcctttat
     2341 aacctttatc tttatacaat ttcacagcac gtgcatgaga aaccatcata ttgtggtgtg
     2401 attggaagac tttggcaaga tcatattgaa tgcctggagg gaatttacca accaagtatt
     2461 gaccatctcc aattgggcca atttcattga aagttgtcca gtagtttact tctgggaact
     2521 cttcaaaaca gaaagcggca taatctacaa agtgttctat attttcgcgg tttaagaaat
     2581 ctccatctga gtgaagaact tctggtgtgt caaagtggtg caaagtcacg aatggctcaa
     2641 catgacgttt gtggcactcg gcaaaaagat tatggtagaa ttcaacacct ttttcattta
     2701 cttcgccata gccagttggg aaaatacgag accaggcaat tgaaatacga ataccattaa
     2761 cgccatattt ttcagctaat tctaaatcaa caggatattt atggtaaaaa tcacttgcag
     2821 gctctgctgt gtaccaataa ttgtcttcaa gatacttatc ccatgcaact gggcctttac
     2881 catccgtgtg agtcgcacct tctgcttgat aagcagctgt agcaccacca aaaataaaat
     2941 ctttaggaag tgttttagtc ataactataa atcctttcaa aaaaagcatt tgagacgata
     3001 ttctgagttc cggaaaccaa ggaaatgaaa tgcaatgata tggttaacaa tattataagg
     3061 aattgaaaga ataatctcgt ctcaaataac ttttacaaat ttattaatca aactgttgtt
     3121 gaacaaatgc aagagcacct tgtccatcac gtgtcaattt gatatattga gctccttcag
     3181 tttttgcaag tttaatgccc aatttatccg tttcagcctt catatcatcg aagtttgaag
     3241 cgacctgtgg agccaagata accaagtcaa actctggcaa catttcacgg tgagcaccgt
     3301 aactacccgc tgccgctttg actggaacat tgtattctgc tgctgctttg ttcaaggcat
     3361 tggcaagcaa gccacttgta cctccacctg cacaaagaac aaggacatta gtttcttttg
     3421 tgatattatt gtttgctgct acatcttctt tagcaacgcc tgcttttcca agaacggcat
     3481 ctgctttagc agtattgaag tttgcagcta ctttttcttt aagagcatcg tttgttttac
     3541 cagaacgttc ttcttcaaga atttgttcat cgtatacctt aacaaatggg taataaatga
     3601 tagtatcaac aactaccaag agtccggcta agataaatga tagaacttgg aagttagtac
     3661 cgagtacaat tcccaacgga ccaggagtaa cccatggaag gttggcagag aatgagttca
     3721 tgttcaatgt gtcaacaaag aatttaaaga tccaaacgtt gacaatcgga gcgaaaatga
     3781 acggtacaaa gaaaatcgga ttcaatacga ttggtgcacc aaataagatt ggctcattaa
     3841 ccccaaagaa tgttggaaca actgaggcac gtccgatggc acggttacgt tctgatttac
     3901 aaatccacat gaacaagaat ggaacaatca atgtagctcc tgttccaccc atggtaacaa
     3961 taaacatttg agtaccagaa gtgataactt tgtctgcatg ttgtccagct tggataagat
     4021 gcaagttggc atcgatattg gcataagtga tagcagcgat tgctggttcg acaattgaag
     4081 gaccgtggat accaacaaac cagaagaagg cataagcacc aaagataagt gtaattccaa
     4141 ggtaaccatc tgcagctgag aagagaggag caagaagggt accgattgat tctgcaacgg
     4201 ttacaccaag agttccctta acaatgagtt caaatccata aaggagaaca accgctacag
     4261 tgaatggaat caagtcttta aatacttgag agatatttgg tggaacctct tcaggcatac
     4321 gaatagtaac attatttttc acacagacct tgtaaacatt aactgtcaca aaggctgcaa
     4381 taaatgctgt caaaagacct tttgttcccg tgaaggctgt caagaagcct ccttcttttg
     4441 caggctcagc tgccatcaag aggaacccta ccatagacgc tagcatcgta gataagaagt
     4501 taatctgatt ggtcgcaggt aggtcacggt tcattgagtc cgtcaaagct ttagcggtag
     4561 taccaccaac aaagaatgct aggataccca tcgaatagct ataaggagtc atcaagaagg
     4621 tctcaatgtc ttttgaccag tggaatcccc aagcatttgg gacataggca ataaggataa
     4681 agatagatga gaaaaggatg actggcatac cagcgataaa tccatcacga atagcacgaa
     4741 gatagatgtt tcgagagatt ttctcaaaga acggtttccc tttctcaata agttcaatga
     4801 gtttatgcat tattttgctc ctcttttata aagttcgatg aggtgatgaa tcacatcttt
     4861 taataagaca gtagtcatta agtggtcctg accatgcatc atggtaacgc tgtatggaag
     4921 ttcctcccca gaagcttctc gagccaacat agctgtctga gaattgtgag cctctgcaat
     4981 acagcttcct gcttctacta caagactatc tgccttagcg aaatcaccat tttcagccgc
     5041 tttaagcgct tctaaaagtt tagagcgagc atctccagca taagcaacaa tttcaaaccc
     5101 taagagagtc atctcttctc tgttcataac aatatcctcc gttttatgtt tttactatac
     5161 tttatcagtc catggactag ctgttttaac aagaactttg ttaagttcat caatattttc
     5221 gaatccagta gtacgcaacc attcacgtgc tgctttttca ccttctttga tgtaaggttc
     5281 tacagatcct gcccaagtag cacgtccaca aagcacacca ttaaacttgg cacctgagtc
     5341 gtgagcaaat tgcaatgttt cttggaacaa tttagcagat acacctgcac ttaggtagat
     5401 gtatggaagg ttagtcgctt cttcttgtgc tttaaagaag tcagcagctt cagctttact
     5461 gtaaactact tcaccatcag caaaaccttc gacatattta acgttaactg gtacttctac
     5521 tttaagaaca tcaatgttaa agcgtggatc agagaaaact ttcatcgctt caatgacttt
     5581 gcgaggtttc acttttgcat attcaacaga acctgcatct gagatttctt catcataagc
     5641 aaggatttcc aagaagaaag gaatatcttc agcgacacat tcagaaccaa cacgttcaat
     5701 ataagcttgt ttttgttggt taagttcatc tgagctgtct acatcatagt aaagcaagaa
     5761 cttaacggca tcagcgcctt gctctttgat acgttttgca gaccaaacat ccaagcagtc
     5821 aggcaaacgt tttgtacttg atgtatcata acctgttttt tcataggcaa gaagaagacc
     5881 tgcttcttta tccaatgctt ttgttgctgg aagtccatat tcagggtcaa gcaacattga
     5941 agaagcgtat tttgtaagtt catcagcaac caaaaccttt agttcttcca tttgagctac
     6001 agttggttct gtatcttgat actgtgccat caaacgtttc aaagcaccac gttggtcaaa
     6061 tgccaaagct gagataacac cgtttttatc tgaaattttt tccaaagatt tgcgtttctg
     6121 ttctgtaagt accattttta tacctctttt actccaattt gtgagtttag tgtttcgtaa
     6181 ttagtcatat tcacatgccc tgtcattgtt tcttgagcat ttaacatacc caatgtcatc
     6241 gcatgtttta ataagtcagc gtcacttttt ttactattta aagctgatgc aataccagct
     6301 accgttgagt cccctgatcc gactggatta acaactggga tatcaggaat atctacctta
     6361 tagaaaacat caccatgttt tgcaaaggca ccgtttcgac ctaatgagac cactatccat
     6421 tcgataccac taaaaagtga ctctttaaga acgtctttga gttcttcaat atcttttgtt
     6481 acttcttttc caagcaactg tgaaagttcc tcattattag gtttgatggc agtaggttta
     6541 gccgacgatt ttagaactgt ttcaagaggt gcacctgaac aatccaaaac gactgctact
     6601 ccttcatccg aagcaagttg gataagtttt tcataataat cgttgggaag tcctgaaggt
     6661 aaacttcctg aaattgtcac aacttctgat tgtttaataa gattgctata gtggtcaaga
     6721 aagccttctg cttcttcatg agaaattgtt ggtccttgtt ccaaaatttc tgtttggtta
     6781 ccctcgtgta agatagcaat acagtttcta gtgtttcctg agattttata aaaagctgga
     6841 ctaacaggag attgttctaa ttcgctttca ataaattcac caattttacc acctaagaaa
     6901 cctgtagcag taactttatc accagattca tacaagactc tagtaacatt taagcctttt
     6961 ccaccagctg tcttactaac atctttcacc ctattcactg tatcaatttt caatgtttct
     7021 agaggataag agatgtctac tgaaggattt agtgtgactg tcagaatcat ttgttatacc
     7081 ttaatcgtgg tagtcaccac ggtcccatct ttcaaggaat tctgtaaaga aatcttcatc
     7141 tgcttgatgt gcattatgtg tttcaacgtg ttcgattttt gcaatcaatt ttttattttc
     7201 ttctgttggt ttgtactcag cttcaatgaa agcttcaatg atgtcattca taagaagacc
     7261 accagtaatc tttccaccga aaccgataac attcgcattt aactcttctt ttgcatatat
     7321 agctgatgtc atatcacgaa ccaacgctga gcgaacacct ggaactttat tgactgcatt
     7381 gttaatgccg acacctgtac cacagataca tactcccaag tctgcttgac cacttactac
     7441 tgcttcaccg acttttttac catagatagg gtagtgagta cgtgcgtggt catatgtccc
     7501 gaaatcaagg acctcatgtc cttttgattt caagaattct gatactgcca ttttttcatc
     7561 tgtgacaatg tggtcacatc caattgcgat tctcattcct taactccttt tcttaacaca
     7621 ttttgttaag catatctact cggatttgat gacgtccgcc atcatattta ccattgacga
     7681 aacctttagc gatgttttta gcaagctcat caccgacgat ttcagcgcca accgtaatca
     7741 ttcgtgaatt gttatgtcca cgtgtcatat aagctgagcg ttcatctgaa acttctgctg
     7801 caaccatacc tttaatttta gttgctacca tgaatggacc agctccataa gcatcaatta
     7861 caatacctaa gttttgctca tctttattta cttcagaggc aactgccaag gtaacatcaa
     7921 caaaatcttg tccgtcctta gtcacatcaa ttacctcaaa accttcttct acaagaaaat
     7981 ttttcacaac atcttttaat ctcgtacctt tgagatctgc accaacaaca atagccattt
     8041 tgaccacctc ctaaatttta gaaagaaatg ctacgtagaa gtagaaaaca ttttgcaaaa
     8101 tgttcgaaaa aacgttttca tcatttc
//
LOCUS       DACECZ010000109         8059 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.110, whole genome shotgun sequence.
ACCESSION   DACECZ010000109 DACECZ010000000
VERSION     DACECZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.110"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(37..885)
                     /locus_tag="IWO35_002261"
     CDS             complement(37..885)
                     /locus_tag="IWO35_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0317303.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSLKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1109..3049)
                     /gene="thrS"
                     /locus_tag="IWO35_002262"
     CDS             complement(1109..3049)
                     /gene="thrS"
                     /locus_tag="IWO35_002262"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ0317304.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3386..3565)
                     /locus_tag="IWO35_002263"
     CDS             complement(3386..3565)
                     /locus_tag="IWO35_002263"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ0317305.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3612..5435)
                     /gene="cadA"
                     /locus_tag="IWO35_002264"
     CDS             complement(3612..5435)
                     /gene="cadA"
                     /locus_tag="IWO35_002264"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0317306.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5713..7992
                     /locus_tag="IWO35_002265"
     CDS             5713..7992
                     /locus_tag="IWO35_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0317307.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         2367 a         1710 c         1456 g         2526 t
ORIGIN      
        1 tattctaaaa aacttatata aaaaatagta aggtttttat ttcgaatcca taaaatattc
       61 ttggattgct tctcgtgttt ttttcacttt ttcgctaagc ttttcgtttt tagccatata
      121 gaaataaaaa atgatatcga tcgtagcaaa ttgggccaaa agtgaattag ttgccgcact
      181 tcgattcgtg atttcttttg gtctagatgt ttgtacgagt acatcggcta tcttgctaag
      241 cgggttattt ccaaattgcg tcaacgcaat gatttcgata ttcatgcttt tcgccagctc
      301 ggctaatgcg actacatctg cacttctacc tgagtttgaa acaagccaaa gcactttctt
      361 tttctcattg ctgacaagtt gcgggagcaa gacgtggata tctttttcaa aaatgattgg
      421 cttaccgaca cgagaccatt tttgcagaat atcttctact actaaagaag aggcgccaac
      481 cccatataaa aaaacagtct ctgccgattc caaacactct acgacttttt ggatcgtttc
      541 ttcttctaga atatctactg tttcttttat cgctacttgt gcattgttac caagtttgtt
      601 tttgagagag taaaaggact catttgcttc tatatcacta aaaccttcat cgattccagt
      661 ctgtagttct gttgaaatcg tgattttgaa gtccgtcaga ctttgaaaac cgattttttt
      721 ggaaaaacgt acaactgtcg gcgcactcaa cccggaagct tctgctaatt cattagctgt
      781 cattgtaggg accttctcaa tattaccgat aatgtagttg gccaactttt tttctgctga
      841 agaataatct tccatcatac tttcaatacg actaattacg ctcataagtt cctccaccct
      901 tttaatgata gtctatgtaa gtataccata tcagaccctt attcacggtc gtatactgga
      961 taaaaaacaa aaaaacaatc cgcccaacag aatatcttat cccatcaatc ccacagattc
     1021 tattgagcat ctgtgtcttt gaggaatacc tgttatctat tgttcggatt gctttctatc
     1081 tactactttt ttacaggaaa tttacctatt agttttctct gctgtaattt ttgacttctg
     1141 cttccatagc atcaataaag atattcaaat cttctacagc tgtttcttta cttccataac
     1201 gacgtacgtt cactgttgca tcttccaatt ccttgtctcc aacaactaat tggtaaggga
     1261 tcttttgtgt ttgagaggca cgtatcttat agcccatttt ttcattacga tcatcaactt
     1321 ctacacgcaa ccccttcatt tgcaaacgtt ctttgatttc ataagcataa tcgccatgtg
     1381 catcaacaga aacagggatg attgttgctt gtataggtgc caaccatgtt ggaaaagcgc
     1441 ctttgtatac ttctgttaaa taagcgacaa aacgttccat tgtagaaaca attccgcggt
     1501 ggataactac tggacgatgc gtattttccc cgtcttctcc aacatatgtc aaatcaaagc
     1561 gctctggcaa taagaagtct aattgtatag ttgataatgt ttcttctgta ccaagagctg
     1621 ttttgacttg aacatctagt tttggtccgt aaaaggctgc ctcgccttct gcttcaaagt
     1681 aatctagttc taattcatcc atagcggctt tcagcattgc ttgggctttt tcccacattg
     1741 cgtcatcatc aaaatattta tctgtattgt ttggatcgcg atagctcaag cggaaacgat
     1801 aatctgtaat attgaagtca gcatatactg ccaccatcaa ttcaagcgta cgtttaaatt
     1861 catctttgat ttgatcagga cgaacaaatg tatgaccatc attcaacgtc atttctcgta
     1921 cacgttgtaa acctgacaaa gcacctgatt tttcatagcg atgcatcatt cctaattcag
     1981 cgatacgaat tggtaattca cgataagaat ggatcgtgtt tttgtagacc atcatgtgat
     2041 gagggcagtt cattgggcga agaacaagca tttcgccatc tcccatatcc attggcggga
     2101 acatgtcttc ttgataatga tcccagtgtc cagatgtttt gtacaactca acatctccca
     2161 ttattggtgt atatacatgt tggtaaccta gactaacttc tttgtctacg atataacgtt
     2221 cgatcgtgcg tcggattgtt gccccttttg gaagccagaa tggcaaacca gaacctactt
     2281 ctggggaaac catgaacaag tcaagttctt ttcctagttt acgatggtct ctttcttttg
     2341 cttcttcacg gagacggata tattctttca aagctttctt gtcaaaaaag gctgtcccgt
     2401 atacacgttg catcatttga ttgtttgagt ttccacgcca ataagcacct gccactgata
     2461 ataatttaaa tacttgtata cggccagtgg atggaacatg tggtccacga cacaaatcga
     2521 tgaattcgcc ttgttgataa gcagtaatca cttcttcttc tggcaattca ctaatcagtt
     2581 ctactttgta cggatcactt gcaaaaattt ctaatgcttc ctgttttgaa agaacacggc
     2641 gttcgatcgg cagattttct ttgacgattt tcatcatttc tgcttcgatt gccggcaaat
     2701 cttctgcagt tacttggttt tgtccattat ctgtatcgta gtaaaaccct gaatcgatag
     2761 ctggtccaac gccaaagtgg atatttggat agaggcgacg catggcttgg gccatcaaat
     2821 gagctgctga atgacggact aagccaagtg catcttcatg gtctggtgtg atgatctcaa
     2881 tacttgcatc ttcctcgatt ggacgagtca aatcaacgag ttttccgttc actttaccag
     2941 ctaaagcttt ttttgctaag ctttttgaaa tgctttcagc aattgccaat gttgttgttc
     3001 cagactcaaa ttctttgact gcaccatctg gaaaagtaat ttttatcata atagtttcct
     3061 ccttgaaatg ttttgattga tcagatcatc aagctgaaaa acaaaaaaat cctagcatct
     3121 cttgaaaaga gatactagga cgaaaagttc gtggttccac ctaatgttca aacgcaaatg
     3181 cgttcctcaa gcgttgtaac ggtccgaccg cccttgcttc aacaagggtt tcgaaagtgg
     3241 tcatcctttg gttttctcct ggaaaagttt cagcctagct tttcctctct tatcgaagac
     3301 tctcaaattc agttggcttt ctcatcaatc aattatgtgt ttatttaacc actaataaat
     3361 agaaaaatca agtgggaaac taaacttatt ttttccattc cccatttaca ccgtatgttc
     3421 cttttttcat ctcattgatg atgacatgga tgtgttcttt tggtgcctgc gtgtttttat
     3481 gaacagcttc cgtgatatct ttcatcatat tttctaattg ttccggactg cgaccttcta
     3541 ctaattcgac atgtacgaat ggcataatgc tctctcctct ttctgatcgt tcttttttca
     3601 actcgattct atcatactat gtttgtcttg ggtactgaat caggaaaaat ggctggatct
     3661 ttttttgctc tcaaggcact caaaatagag accgtatcca cgacttcttg caaaacagct
     3721 ccgaataatg cggggattag tcccgtactt gcaattaaca tcaaaaaaat acaaataaag
     3781 atgccaatca gtacagactg tttcgctatt ctcatcgtat ctcttgaaat cactactgct
     3841 tttgctacac gcaagagatc atctttcaag atgaccgcat ctgcactttc actagcagct
     3901 gtagaaccat gagcccccat tgcaataccg atatctgcag cagcaagtgc tggtgcgtca
     3961 ttcacgccgt ctcctaccat cgcaacaggt ctttgatctt tagggatact ttctaaaatg
     4021 gtcagtttat cttgaggcaa gctacgggca tgtacagaat ctaattggac tttttctgca
     4081 atcgtttgag caatggcaga tccatccccg gtgatcatca tcaaattttt cacatcaagt
     4141 tttcttagct ccttgattgt ctttgctgct tctgggcgaa tctgatccga aaataggatc
     4201 gttcctgcaa aagatcgatc aattgttaca tagattgctg tattggacgt ttctatttct
     4261 tcagctgaaa caaaagaggc acgtccaatc tttacttcat gattattgac tactgcttgg
     4321 attccttgac cggtcacttc tttcagttga ctaacaggat acagttggct gtttttcacg
     4381 ttgttcacta acgaacgagc taagatatgg ctagattcct gttctgcact agctgcatac
     4441 agaagtagct cttctttttt tattccattg actggctgaa tcgcctctac ttccaatatc
     4501 cctttggtaa gcgttcctgt tttatcaaaa gcaatcgttt ttgtacgtgc gagcttttca
     4561 atcgttgttc cagtttttac aacgatcccg tttttacttg aacggctcat tcctgcaact
     4621 aaagcaatgg gcgcagctag aatcagtgga caaggtgatg ccactaccag tacctcagca
     4681 aatcgtaaag ggtcttttga gaaccaccaa gaaataccac cgatcaaata agcaatcaac
     4741 gtgaaaggga ctgcataacg atcagctaaa cggacaaatc gggcaggctt ttcttttgat
     4801 tcttcgacta gtttaataat catttgatac tggctgtctg cggcacgttt ttcagcgatc
     4861 atttggatcg atccgtctcc atttaaagac cctgacataa tctgatctcc aggtttcttg
     4921 gtttccgggc gggactcacc tgtcaaagaa gattcatcga ccgaagattc tcctttggtc
     4981 actcgaccat ctgcgggtac aagttcgcca ggcttgacta gtaactggtc atcaatattt
     5041 atttcatcga ttactatatc ctgcaatttt ccttctttga atcgatgggc aattcttggg
     5101 gtgttatcta acaaagactg cagttcttta cccgctcgtt tattcgcgta atcctctaga
     5161 ctatctccgc ccgtaagcat gatcaatatc atcaaactag cccagtattc tttgacggct
     5221 aatgttgcaa ggattgcggt aatcgctaat acatctacgc catatcttcc tgaacgtaac
     5281 gtcttgatca tttccaaaaa catcaagaag gcgagaatcc ctccagtaaa tgcgacaatc
     5341 gcaaatgcaa gggccggctg gtgaaaaaca aattcactta ataaagctat cccaccaata
     5401 agcaaagtaa gtaaaaattt tttgaaatgt gtcattgttt tttctcctct tttatgtgac
     5461 ttagagaggt tagtaataaa tttccagctg cctctttcat cttaagcata gtccttgact
     5521 agcacttttt aaaacaatag gccttaattg aaaacaacta tcactatcag cgtttccaaa
     5581 attcatcagt tctacaaaca aaaatattca gcaaggatta ctttttcgat tcatcagtta
     5641 aaaagtacct tttttcgaaa aactatgcta tacttttaat tcgttattta cggcggaagg
     5701 agcaaacatg cgatgaatga gaaacaacta gaacaaacgc acgtggatga aacgatcgaa
     5761 ctaatccaga tcgaacaaaa tatgctggat acaaaacaac aagaattgac tcaggaaatg
     5821 aatcattcga caaaagacac agctaatcac aagattcgag ctggttccaa tgaagctttt
     5881 tatgaatctg ctgttgaata tcgccaacat gagcaggaat tgcttttgaa ataccatact
     5941 cttgaagcac agcaaaaacg cctgcaaacg ttgtctgtca tgaaagatag tccttatttt
     6001 gcccgtatcg actttaaaga agaccgtgaa acagaaacat tatatctagg tatcgcttct
     6061 ttaagagatc tggcagaaga aacaatcgtt attgactggc gcgcaccaat tgccaatctt
     6121 tattatgaag gtgagattgg tcccgcattt tatgaaactg aaacagatag aatagatgtc
     6181 gatctgcagt tgaaacgaca atttaaaatc caagaaggac gtattttatc tatggtagat
     6241 acgtcggaag taatcaatga tgactttttg ctagagatat tggatgaatc atcaagcaac
     6301 catatgaaaa acatcgtctc tacgatacaa aaagcacaaa atgcaatcat tcgggacaca
     6361 acaagtaaag taatgctgat tgaaggaatc gccggaagtg gaaagacatc cgctttgctt
     6421 caacgtattg cttttctgtt gtatcataat cgcaagtggc tcgaggctga aaacgttctt
     6481 ctattttcac ccaaccattt gttttctgat tacatctcga ctgtattgcc ttcattaggt
     6541 gaaagcggcg tgcctacaca gacattcaaa aattatttgg tgcagctgct gccagagttt
     6601 tctttactag atgaacagca acaagaaagt ggttttttgc taggggaaaa agatccgata
     6661 caagtgatga aaagcggatt gaccttagtc gatcagattg atcgctatat ccaaggcatc
     6721 acaaactacg gtcccttgtt ccgtgatatg aaaataaatg gccgtacgat cctatcaaag
     6781 gaatcgattc gccaatggta taaagaaaca aatgaacaat tacctttaca tcatagactt
     6841 tctctattac aaacgaagct cctgaaaaaa ttaggcggtc tgcaaaaaga cgaaacacgt
     6901 cagcaatggg tcaaagatct ggcggaagaa cagctccaag aactatacgc aacagatcca
     6961 aatcttgaat atacagaaca aaaggagcgt tcattacgta aaaaactggc caaccaaatc
     7021 gtacgtaaaa gattccgtcg aatccatcga gggatcatga attatcaatt tgtcaatata
     7081 acgaaacaat atctccactt tctacaaact gtgccacttt ctcttctaac acgacataaa
     7141 atcagcgaga ccaggtggca ggaacatttg acaactgttc gtacacactt gcgtcaaaga
     7201 gaattgcgtc aagaggatgc tgtactattc tttttactga tgcgccgaac atatccagtg
     7261 acggttacac acaaagctcg ctatatcttt attgatgaaa tgcaggattt cgcaccatca
     7321 caagtggcat tgctgcaagc cttgttccca aaagcaaatt tgactttctg tggagatcta
     7381 aatcaaaatg tctttggaaa tgaaacgatc gttggatcat tggaagacct tttcgtggac
     7441 cgtgaagtca ctcgtttcca attaacgaca agctatcgct ccacaaaaga aatcactgat
     7501 tttgctaatc attttattgt gaaagaacat caagtagaaa caacagcaag aaaaggagac
     7561 cttcctttgc tcatccaagg aacaacagat gatatcaaat tgaattggtt agcagatacc
     7621 attcgaacga ctcagggaaa agcccggtat tggcgtacag caatcatagc taaaacacaa
     7681 gaagaatgcg aagctcttta tgctcaactg ccagacgagt tgaaacaaca agtccagctg
     7741 attttaaatg agagtgactt tatgaagcgt tccattattt tacttccagc ctttttagca
     7801 aaaggtctgg agtttgaccg agttttcttg tgggatgtag atgcagaaca tttttcttct
     7861 cagcaagaca aattgatcct ttacaccatg tgtacacgtg cgatgcatga attgaccctt
     7921 tttacctcca ataatgaaag tcctttgtta aacaatatgg atatttcatc ttatgagaaa
     7981 aaagtactgt aagtaaaaag acaactgcta tcattaatgt tagcagttgt ctttttattt
     8041 acaataataa ttgcagaaa
//
LOCUS       DACECZ010000110         8050 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.111, whole genome shotgun sequence.
ACCESSION   DACECZ010000110 DACECZ010000000
VERSION     DACECZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.111"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..769)
                     /locus_tag="IWO35_002266"
     CDS             complement(<1..769)
                     /locus_tag="IWO35_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAQ0317308.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNAL"
     gene            complement(974..1324)
                     /locus_tag="IWO35_002267"
     CDS             complement(974..1324)
                     /locus_tag="IWO35_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317309.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            1919..2365
                     /locus_tag="IWO35_002268"
     CDS             1919..2365
                     /locus_tag="IWO35_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ0317310.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            2989..3981
                     /locus_tag="IWO35_002269"
     CDS             2989..3981
                     /locus_tag="IWO35_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0317311.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            3997..4881
                     /locus_tag="IWO35_002270"
     CDS             3997..4881
                     /locus_tag="IWO35_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0317312.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            4930..6537
                     /locus_tag="IWO35_002271"
     CDS             4930..6537
                     /locus_tag="IWO35_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0317313.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            6538..8022
                     /locus_tag="IWO35_002272"
     CDS             6538..8022
                     /locus_tag="IWO35_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ0317314.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
BASE COUNT         2517 a         1281 c         1705 g         2547 t
ORIGIN      
        1 ctaaagcatt gtaaaaaaat ggacgtctaa ctatatattc gcttgtatat agtatatctt
       61 gattcgtgga atcatagggt ttctttacca tctttttggt gacagttggt agtttgagat
      121 tccgggactt gaaaaaagct tttttcttat gctctttcaa ttccagctct ttatcgaaat
      181 atttgctgat atagcgcccg cgattttcga tactgtctac atcgatcttg ttgatcttaa
      241 cgaaaccctg accccaaatc gtagttaacc ggttaaatga gataaagggg aaatcaaaca
      301 agataatatg gtagtggatt gcaccgcgtt gctgcttttc ccacgtggcg agatacttta
      361 aatttgcttt tttagtttta tagaggtcat aattcagtcg tttgataaat tttttaaatt
      421 cattatttgc atactcaatg tcttgaatgt tttctttgaa agtgagagtt aaaaatttcg
      481 tttgattgtc aaaattcgtg tctacaaggc gggcaatctc aaatcgcata ctttgataat
      541 gtttctgttt gcgttttaaa ctgtcatatt gtccttgagc ggataattca tcatagtttt
      601 ttcttttttc aagagtaata tcattttcat aaatccaagc taatttttgt tttcgttcca
      661 gatttacttc gttttcgatc gttttacgtt tgggagtaat gattgcttca taatcataaa
      721 tttcaatata cgtgggagtt tcaatgattt tctgattata agcttgcaaa aatcatcact
      781 ccttgttgcg tattcgattg tcaaagaacc agtcattttc tcctgatgtt gatctataaa
      841 tcaagttaag ggggaaggcc tgcgtagcag cccttctagc ctatggggtg ttgggcggtc
      901 ttaacggaca gaaacggctc tgtgggcgct gccccgccgt ttcgtcctaa ccacccaaca
      961 ctccatagta tagttagttt tgtgtagtgg taccttgttt tctgggaatc aaagttgccg
     1021 tatcagcggt tgccgataac ccataactga caaattgtcc attcgagcgt ccccaaggta
     1081 caatcaccaa attggtaaat gtaattatgg ctaaaccttg ttgggtaagt tcttccggcg
     1141 tgatcacagg atgttcagat ttgactttga cactgatttt ttcgtagcga agttttggta
     1201 ggatacattc gtagctccaa cctagaattt cgccctcctt ttgccagcga gtgacattga
     1261 cgacttggta ttctttttcg agtgtttctt ttggtaaact gagatcggta atttttattg
     1321 gcatcacaat tctccttttt tatacgaaag tttatacgat ttgattaaaa aatatagatg
     1381 gccatctacg acttgtatta tatacgagaa cttatacgta gtcaagagat tttctaaaaa
     1441 aatatatacg attttttata cgagtgttat aatgaaaaaa atgataagga ggaatttgaa
     1501 tatgtttggt ttagtacaac ctaataaagt gaaggtgggc caacgaatca aagaaataaa
     1561 agacggaatg tctctctctt ttacagaatt agggaatcga ttaggtttaa aaaagccgac
     1621 gatcagttcc tacgttcaag gctatgcgtt ggctcctaag agtgtgatca accaattatc
     1681 tagtatcagc ggaaaacctg tcggctggtt ttattttggg catgtggaag aatatatccg
     1741 agagtatttg tttctcattg gtcagaaaaa aatagtggac gattatcctg aaatcattga
     1801 gaaaataaag caagaatttt atacgggcga gttcaaaaat cctgcttggg agaatgagct
     1861 aggctatcct ttggaggaat ttatccaaga atgttttgta gaatattcga gtgaaatcat
     1921 gaaggactat gtcgtacaga ttacgaaaga ggtacttgaa gaatccaaaa cgctaaacaa
     1981 actatcatca aaagcaaaag aagaagctgt cacgattctt tcatctggga tcattgacta
     2041 tatcgagatg agtggtgaga tcaagtatgg agagaaagat cgcattgcga aaatggtgaa
     2101 agaatcaata aaaacctttg atgtgaaaca agacctgaca tttaatgatt cctatttgat
     2161 tggaaagttg atcaatattc ttggtacaga tgaagatacg gagctgtttc tttcttcttt
     2221 atcaatgata tttactgata agcatttttc cactcgattt ggtggggaag aattagtcga
     2281 aatatttcaa tcgatgcgac cagcgttaat caaactctat cgggagaaaa caccagatga
     2341 attttatgat tggtttgaga agtaaaataa gaaaagtggc ttagattaca agttggtgaa
     2401 aaaatttttt tcagcataac atcagtaatc tataaagggg ttgttgatta tactgtagaa
     2461 gttaagtact caagagattt tttgtacgta aacaaagcaa aaaagggtgc agtttcacta
     2521 gaaaatgtac tcttttttgt gatttttctt agttatagag tttttttgaa actttaaaaa
     2581 tgagaaaaca tattgatttt aaagagtgta gacatagctt cttttgctaa atagattaaa
     2641 cttactggtg agtggtcttg aacatacttt taaatcacat tttactgagt tttattggat
     2701 tttataattc attggaaacc tcgtacttaa tctgttttta tctatcgact agaatctgta
     2761 tatattccag ctatcaatga ggccgcagaa aagggtgctt aaaaataatc tgttgtttaa
     2821 aatattatta tcagaatact tagatacaaa attctggcac aaagtaaaaa aaatattgac
     2881 ttaaaaaaat ggatatggta atctataaac gaaaatagtg gtaacgtttc caaaatgcat
     2941 cttcgttaaa tttttatttt tttaaacgaa aatggaaacg tttctaaaat gtattgggag
     3001 gagattgaat tggaaaagac attgggacca ggtggtgaga taagggcggt acaacgggat
     3061 aaaaaaaata tttcgaagtg ggaaaaatta aagaaacagt tgcggcgtga taagtggatg
     3121 tacttgttgc tcttaccagg tatcatcatg acgtttatct ttaagtatgt gccaatgtac
     3181 ggtgctgtga ttgcatttaa agactataat cctatgctag gaattttgga aagtccttgg
     3241 gtaggactag atcactttag ggaatttatc agttcaccaa attttacgct tttgctgagc
     3301 aacacattaa aaattagtat ttatggttta ttgttaggtt tttttccacc aattatttta
     3361 gcactgagct tgaatcaaat tatcaataaa aaaataaaac agaaaatcca gttgattcta
     3421 tatgcgccga attttatctc agtagttatt attgtgggga tgctgttttt gttcctatcc
     3481 gcaaatggac ctattaatgg gttagtagaa aaggttacgg ggcaaccaat tatgttcatg
     3541 tctaatccag cttatttcag aacgatttat attttatcgg gaatttggca aggaatcggc
     3601 tggtcatcca ttctttacac cgcaacgctg gcgggagtca gtaccgattt atatgaggca
     3661 gcaaatatcg atggggcaaa tattttacaa aagatttggc atatcgattt gcctgcattg
     3721 aggcctgtga tggtgatcag cttcatcttg gccgccggcg gtattatgaa tgtaggatat
     3781 gaaaaagcct acttgatgca aacttcaatg aacatccctg cctcagaaat catttccact
     3841 tatgtataca aagtcggctt gcaatcaggt gattatgcct actctgctgc tgttggtctg
     3901 ttcaatatga ttattaatgt cgttttatta ctgattgtca acaatataac caagcggcta
     3961 aacgatggtc aaggcttgta atgaaaggag aaaattatga atagtgcttt atcaaaaatc
     4021 gaccgtcaaa tcttatggtt gaatcgagtg attgtaactt ttttagtcgt catcaccatt
     4081 gttccgctga tttatgtact ggtagcttct tttatggacc cagctacttt gatcaataaa
     4141 ggtataagct tcaatccgaa agattggaca atacagggat atcaacgggt atttgcagat
     4201 gattccattt tacgcggttt tgtaaattcg ttgttttact cttttagctt cagcttattg
     4261 actgtattta ttacgatgat cactgcgtat cctttgtctc gacctaactt ggcggggaaa
     4321 aagattatta tgactttttt tgtcattaca atgttcgtgg gtggtggctt gatacctacc
     4381 tatttattgg taaagaactt gggcatgtta gatagtgtat gggcgattat cgtaccaggt
     4441 gctataaatg tgtggaatat tatcttagcc cgtacctatt ttagtgcttt gccaaaagaa
     4501 ctttctgaag ctgctactat cgatggcgcc aatgacttgc aaatactgtt caaagtcatg
     4561 cttccattag cgaaaccaat tatgttcgta ttgtttttat atgcctttgt tgggcaatgg
     4621 aactcatact ttgatgccat gatttacatc aaagatcccg acttacagcc gttgcaactc
     4681 gtattaagaa atattttgat ccaaaaccaa ccttcacaag acatggtggg agccaatact
     4741 gccatggcag aaatgaaaca aattgctgag ttgatcaaat acgcaacgat cgtcatttct
     4801 agtctgccat taatcataat gtatcctttc ttccaaaaat attttgataa aggtgtttta
     4861 gttggttctg ttaaagggta agtattgttc actatttgta tgaataaaaa ttataaagga
     4921 gattttaaga tgaaagcaaa atggatgctg ccaatcgttt ttacttccgt actaattttg
     4981 ggggcttgtg gaggctcaaa agaaaagagt aaatcaagcc ctgattatga actagaaaat
     5041 gtctcttttc cattgaaaga aaaagtatct ttaaaaatga tgtctcaaag tgcccctttg
     5101 gctcccaatg atcctaatga caaattgatt ttccaacgat tagaagaaga gaccaatctc
     5161 catattgatt ggacaaacta cagtagtgat tttgccgaaa aaagaaattt ggatatcgct
     5221 agtggtgatt taccagatgc catcttcaac gccgctgcag gtgactatga tttattaaac
     5281 tgggcggaaa gtggcgttat tgtaccgatt gaagatttga tcgaagatta tatgccaaat
     5341 ctgaagaaaa tttttgatga aaaccccgaa taccgacaaa tgtctactgc acctgatggt
     5401 cacatttact ctatgccttg gattgaagaa ttaggcgaag gaaaagagtc gattcataca
     5461 gtaaatggga tggcttggat caacaaggag tggttggata atttgggtct tgaaatgccg
     5521 caaaccactg atcagttgat gactgtgttg gaggctttca agacgcaaga tccaaatggt
     5581 aatggtgaag cagatgaaat tccttttagc tttattaaca atggaggaaa cgaagactta
     5641 aaatttttaa tgggggcatt cggtctaggt gataatgatg accacttagt cgttgataac
     5701 gatggtcagg tacaatttac tgctaacaaa gaagggtata aagaagccat caaatatttc
     5761 aatgaaatga ataataaagg tcttattgat aaagaggctt tcgaacaaga ttggaatacg
     5821 tatgttgcca aagggaaaga acataagtat ggcgtgtact ttacttggga taaagcaaat
     5881 atcacgggtg cagatgatag ttatgacgtc ttgccagtct tagcgggacc agatggaacg
     5941 aaacatgtta ctcgtacgaa tggcatgggt ttctcaagag atcgtttcgt aattactagt
     6001 gcgaataaaa acttggaact tacagctagg tggatagata aaatgtatga accgttacag
     6061 tctgtccaaa ataactgggg aacctacggt gatgaagcac aggcgaatat ttttgaatta
     6121 aaggatggta tgttaaaaca tttgccttta gaggggacag caccggggga actaagacaa
     6181 aaaaccgaag ctggaggacc attagcagtt cttgattctt attacggcac agtcactact
     6241 atgccagatg atgcgaagtg gcgtttagat ttgatgcatg acacgtattt gccatatatt
     6301 acagaagaca atatctatcc acgtgtcttt ttagaaaaag aagaaacaga tcgattagca
     6361 aaaatcgaaa cggatatgtg ggattacatc tatcgcaaaa gagcagagtg gatcaccggc
     6421 ggtaaaatcg atgaagaatg ggaagaatac cttgctgaat tggaacgtgt aggtttggaa
     6481 gaatggattc aaataaaaca aactggctat gataattttt tgaagggagc acaataagtg
     6541 ttagtagaag aaacggcgat tttggataaa gcaaatcgat ttattgaaat gatgcgagga
     6601 aaagttaacc ctcagtatcg acaaaactat catttttccg cacccatcgg gtggataaat
     6661 gaccccaatg ggtttatata ttacaaaggg cattaccatc tcttctatca atactatcct
     6721 tacgatagtc gatgggggcc catgcattgg gggcatgcgc gcagtagtga tttggtccat
     6781 tgggaagatc taccagtagc gttagcccca gatcaacctt atgacagcgg cgggtgtttc
     6841 tcaggaagtg caattgaaag agacggaaaa ctatatttat tttatacagg ctgtcaagaa
     6901 attgatggta ttagccatca gcatcaatgt ttggcggtct ctgaagatgg tattcatttt
     6961 agcaaataca gtggtaatcc tgtggtagca gaaaaacagc taggtgaaaa tgggctaata
     7021 caagaatttc gtgatcctaa agtaattgtt cacaacgatg gttactatat ggtagtagct
     7081 actaaatcta cacagtctac tggtagaata gtgatgtttc aatcgtcaga tttgtatact
     7141 tggcactttt actctgtgct gctagaaggg acttcagagc aaggaataat gtgggaatgt
     7201 cctgatttat tccatttaga cggacgagat gtcttaatca tgtctccgat acaaatcaag
     7261 aagaacggac tggaatatca taatatcagt tctaccatgg cttgtattgg tgaggtaaac
     7321 tgggaaacag gtcagatgat agttgataat ttccatgaaa tggattttgg aatggatttt
     7381 tatgcaccac aaacaacaag agatcctaat ggtggacggg tgatgattgc ttggatgcag
     7441 atgtgggatc gtacattgcc tacacatgag ttaggccatc tttgggcagg ctctatgagt
     7501 ttgccccgag agttaagagt gaaaaaaaat cgattggtac aaaaacccat ctcaactgta
     7561 tatagtcatt tagtttatca acaaggcagt gaaaatattg cagtgactga cacatcagtc
     7621 gtatttcgaa acgcgctgtc tgattcttca tatttacatc tagtcgcaga tttggagaaa
     7681 gctgaagttt ttacaattga attactgcgg tctaagacgg attgtctcaa actagcctat
     7741 gacactgcta cacaggtttt tgaattggat cgtcagaaac taggaagaga gcttacaggg
     7801 gcggaagaag accctctgaa tgtacgaaaa gttgaagtac ctttgattga taatcagctc
     7861 gttcttgaga tttttagaga tacctcatca attgaagtct ttattaatgg gttagaagtt
     7921 atgacggcta ctttctatga gctggaaaaa gctcgagata tcttgtttca ttcgatcgga
     7981 gaaagtcgta ttcaagcttt tgaaacagcg agtgtgaaat aaatgtttga ttgcttaggc
     8041 gatgaatcaa
//
LOCUS       DACECZ010000111         7837 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.112, whole genome shotgun sequence.
ACCESSION   DACECZ010000111 DACECZ010000000
VERSION     DACECZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.112"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..813
                     /locus_tag="IWO35_002273"
     CDS             <1..813
                     /locus_tag="IWO35_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAQ0317315.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            807..1229
                     /locus_tag="IWO35_002274"
     CDS             807..1229
                     /locus_tag="IWO35_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317316.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2399..3367
                     /locus_tag="IWO35_002275"
     CDS             2399..3367
                     /locus_tag="IWO35_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0317317.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3384..3908
                     /locus_tag="IWO35_002276"
     CDS             3384..3908
                     /locus_tag="IWO35_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ0317318.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            4084..4488
                     /locus_tag="IWO35_002277"
     CDS             4084..4488
                     /locus_tag="IWO35_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ0317319.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            4505..5653
                     /gene="tnpB"
                     /locus_tag="IWO35_002278"
     CDS             4505..5653
                     /gene="tnpB"
                     /locus_tag="IWO35_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ0317320.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            6175..6456
                     /locus_tag="IWO35_002279"
     CDS             6175..6456
                     /locus_tag="IWO35_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317321.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            6458..6874
                     /locus_tag="IWO35_002280"
     CDS             6458..6874
                     /locus_tag="IWO35_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317322.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            7496..>7837
                     /locus_tag="IWO35_002281"
     CDS             7496..>7837
                     /locus_tag="IWO35_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAQ0317323.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2944 a         1320 c         1304 g         2269 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgaa
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaaac
     1441 actttagtga gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt
     1501 ccgtaggacg tactgaaagg gtgggattta tgagcgaact aatgcaaaat ttggctagca
     1561 aaaaaagtta aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aagcagttta tttcagcttt gtcatggcgt gtgcctagac
     1681 ttttaaaaaa gtcggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaaga
     1741 cgtttgaaac gtctaagtat ataaatatat aagagtaggt ttaagagtgc ttaaatcgaa
     1801 ttttagaggg cattcagaca actttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acatttttag caaaggagcc ggaagcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcgactgta gggagtaaga
     2041 ttcccagttg agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctata tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacagg
     2341 cattttgtat ttcataacgt gtatctcttg tgcgagtaag aaaggagcta ttttaaatat
     2401 gtctaatgaa atcgtaaagt atcatcatga attaaataca attccgttgc gaaaatttac
     2461 accagtcgaa atgaatttgt tcttttctat cgtatcaaga atgcgtgatg taggtgataa
     2521 aaaagtacaa tttacattcg agcaattaaa agatttaagc aattataaag caactgcaaa
     2581 tgttcgattc atagatgatt tagaaacaac gtatgacaaa ttaatggatt tacgttttgg
     2641 tagaagaagt gctgacggtt tgcaacgtga acgttttgtt ttatttaatc aatttaaaat
     2701 cgacggaaaa tctgatattc cttttgctga aatacaagtt catgaaaaag ccttaccctt
     2761 acttaataat cttgaagaat gggtgcgcta ttctctccaa caattcaatg aattagaaag
     2821 cagttattca aaaacaatgt ttcgattact aaaacaatac agaaccaaag gtttcgctta
     2881 tttttcaaaa gaagactttc atgaattact tgatattcct aaaagttaca accaaggtac
     2941 gattgatcaa cgtgtgttca atccaatacg acaagaacta accgcaatct ttaaaggttt
     3001 aacaatcaaa aagaaatacg gaaaaggacg gggaaagcct gtcattggct atcaatttac
     3061 atttaaacct gagataaaaa aatcagacga ctttaacaaa catgccccaa caaaagaaga
     3121 acaaaacaaa gcattagaac tagctaacca acaagttcaa tctgaaaaag cacctacttc
     3181 attacaagaa aaaatcaaag aacaaaagcg aaaattggaa cttttacaac agttagaaaa
     3241 gttagaaaga gaacaaaata cccaaaataa agaaaataat gcacaaaaat cagagataaa
     3301 ttccgatatt ccttcagaac taaaaaaaga cttattagac aatcttcaaa acttattcaa
     3361 agattgaggg gtttttagat tttatgacag aaaaaacaat caaagaactt gctgaagaat
     3421 taggagttag taaaacagca attaacaaaa aagtttctga cgaaaatcga aaactttggt
     3481 tttcgaaaat cggaaacaag tttgtgatta atgaaaccgg acaaaaggct ataaaatcaa
     3541 tgttttttaa cgaaaacgaa aactcaaatc aacaactagt tggcgaaaaa acgaaaaccg
     3601 aaaaccacaa aaacgccaac caagttcccg aattagaagt cctttctctc ctagaacaac
     3661 agttaaatca aaaagatatg caactacaag aaaaagacga gcaaataaaa caaatgcaaa
     3721 aattactcga ccaccaacaa caactcaccc tgcaagccaa ccaacaaatc gagaaactgc
     3781 aggaacaact acagttgact tatacggaag aatcttcgga aaataaatca acgactcttt
     3841 ctgaagaaac agaatcgata gaaaaacaat caaaggcgga gaaaaaatgg tggcaattct
     3901 ggcaataaaa ccagcaattt aacagaatta ggcagaagtc gcccatcctc tataggtggg
     3961 tgatgaatgc cgttcggtat gagggttacc gttggtaact cgaagaccga caactaaggc
     4021 gtattgcctt tttattttgt cccatgttac aatcagtcta tacaagaaat gagttgatat
     4081 aacatggaat tagatacaaa taatcattca gtatttcttc tttattacca ccttgtttta
     4141 gtaacgaaat atcgtagaca agtgattgat gatgaaatat ctgattatgc caaaactact
     4201 tttgaaagaa tttcagaatc gtatcacatt actttagtgg agtggaatca tgataaagac
     4261 cacgttcata ttatgttcaa agctcaacct aaaacagaat tgacaaaatt catcaatgcc
     4321 tataaaagtg ccagctcaag attgataaaa cgtgactttc caagagtcaa acaatttctt
     4381 tggaaagaaa tgttctggtc taaaagtttt tgtcttctga caactggtgg cgcaccaatt
     4441 gatgtgatta agaagtatat ccaaaatcaa ggaaacaatc ataaatagaa agtaggtgaa
     4501 ctttatggaa caactaaaag catacaaatt cagaatctat ccaacagagg aacaagaaat
     4561 cttctttgcc aaatcttttg gttgtgtccg taaggtctac aatctaatgc ttgatgaccg
     4621 aaaaaaggcg tatgaagaag ttaaaaatga ttcttctaaa aaaatgactt ttccgacacc
     4681 agctaaatac aagaaagaat ttccgttttt gaaagaaatt gatagccttg ctttagccaa
     4741 tgcccaactt aatttagata aagcatacaa gaattttttt cgggataaat ccgttggatt
     4801 tccacgtttc aaaagtaaga aaaatcctgt tcagagttat acaacgaata atcaaaatgg
     4861 tacagttgct ttgattgata gtaaattcat caaagttcct aaattgaagt cattagtaag
     4921 aattaagctt catagacaac caaaagggat tatcaaatct gctacaatat cacgtcattc
     4981 tagcggtaaa tactatattt ctttgttgtg taaagaagaa attatcgaat tacctaaaac
     5041 taattccaca attggtattg acttaggtat tatggacttt gcgattcttt ctgatggaca
     5101 aaaaattgat aataataaat tcacgtccaa aatggaaaag aaactaaagc gtgaacaacg
     5161 caaattgtct aggcgtgctt tattggctaa aaataaaggt accaatctat ttgaagctaa
     5221 aaattatcaa aaacaaaaac gcaaagtagc tagattgcat gaaaaagtaa tgaatcaacg
     5281 tactgatttt ctgaataagt tgagtacaga aatgatcaaa aatcacgata ttatctgtat
     5341 tgaagactta aacacaaaag gtatgttgcg aaatcataaa ttagctaaaa gtatttctga
     5401 tgtttcttgg tcaaagtttg tgactaaatt acaatacaag gctgactggt atggacgaaa
     5461 aatcatcaaa gtagataaat ggtttccgtc tagtcagatt tgttcagagt gcggacataa
     5521 agatggcaag aaatctcttg aaattcgaga atggacttgt cctgtttgtc atactcatca
     5581 tgatcgtgat atcaatgcta gtatcaatat tctgaccgaa ggtctaagac tacattctat
     5641 gggattggct tagatccaat catcaagaac cgtaggaact acggggatag cttggtaaat
     5701 aagagacacc gctgttagta aagaaatacg ctatcaagta tgctctattc ccaagaagct
     5761 cccacttcaa gcgttaagaa ccgtcaggtt tagctaagtg gtgagtagtt cacattaata
     5821 aaaattgctg gttttattct tctttttgct tttgcgagca gagcaacatg gacccatgtt
     5881 gcgatatttt ggtggcgccc caatccccgt tttttcgttg aatcttactc ggactgcact
     5941 cgttttacag ctgatttttc tgaattttga aagagcataa ggaaacgtgg tatcactttc
     6001 ttacgaaagg tgctagatag gctgtgccta tcacgtttct cagaaaaaaa tttgctatca
     6061 cttttttgcc attgtggcta ggctatgtat agtgttttgg cgaaaaatag ctatacaaat
     6121 actatatttg tatggtatac ataaattata cgctgatacg aaaggaaaat ttccatggac
     6181 attttagttc gaaatatcaa tcccaaaatt attgcaagaa tagataaacg ttgtcaggaa
     6241 ctcacaacca aaacgggcaa aaaatggagt cgaaataaat atctaaaagt tctaatcgaa
     6301 agtgattttg accgtcaact tatgaattac aaaaaagatc aattcgattt attactcgaa
     6361 aaatttattg ccattcaaac agaaaatacg aacatactga atgactatgt tcacacaaac
     6421 aaccaactca ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa
     6481 acacgtaacc ctttatccca gtcacgttga cgttttagat tccatcatcg aaaaaaatcc
     6541 agacgtcaac aattattcag aagccattcg ttttttatgt atgagttatg aacctgatac
     6601 caatacaacc acaaccagta accaagcaaa actcaatgcc atgggaaaag aaatttctat
     6661 catgacagaa atcatggcag aatttgctga tttatatcta aaagatactg gtgttcttat
     6721 cggtacaaat tctaaagtgt atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc
     6781 ttcccgacga aatcaaccca aaaatcgtca aaaagaaacc aatcaaacct ttacttctgg
     6841 aaattttgac ccaacaaact ttctaaaaag ataaaaaaag aaaccgatcc ctaaaaaaat
     6901 tcctcgattt ctttttaggg atcagtcttc cagcaagatt tgcaagttgg taccaacttg
     6961 cgaaaaaaaa gaaaaatccg cttatgcaac ttcttctttt tgtattttga ggcgctaccc
     7021 caatccccaa gtatcggtta tttaatagcg cggaaatcca aagaatagga cgtaccctga
     7081 aacaaaaaaa gactgctctc acgagcagcc caaaatgaga ttatcctatt catcaacata
     7141 aaaatattac aaagtgaaga actagagaaa gacaaaaaaa gcatatcaaa tcctctcaaa
     7201 aataacaagt ctttaaaaaa tgacagataa aaatgcacaa agaactaaaa aagagaaatc
     7261 gcgtaaaatc aagcattaga agctagtaac tagctagacc aattaaaaat cacaaagtga
     7321 gcgtctctat ccattggtag aggggctact tggcatagtg tacattatgt taactaacgc
     7381 tataaataac gattatgttc ccaaaacaat attttatgaa ctataacaaa aatttgttat
     7441 cgcatattgt atattttaat tataggtgta taccacttaa atttaacaca aaggcgtgat
     7501 tattttgaaa cccaaaaaga aaatttatct aggtctcctc ttttccttga tgattggaat
     7561 ttttagtata ggaattacaa atacctatgc ttcagaaaat tctaatttca atatcaactc
     7621 ccaaccaatt gaagaacaag caattaattt tgcgaaaact gattttgaac actccataac
     7681 ttcaattctt ttaatggaaa ataacaccga aactgatatt aactatactc ttggttcgcc
     7741 atttaaaatg ctcgatggag aaaatacggc aaatagttct attttctttc cagtattttc
     7801 agacgctgat aatacgatta aatatattta taccgtc
//
LOCUS       DACECZ010000112         7799 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.113, whole genome shotgun sequence.
ACCESSION   DACECZ010000112 DACECZ010000000
VERSION     DACECZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.113"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(12..2183)
                     /gene="copB"
                     /locus_tag="IWO35_002282"
     CDS             complement(12..2183)
                     /gene="copB"
                     /locus_tag="IWO35_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ0317324.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2203..4389)
                     /locus_tag="IWO35_002283"
     CDS             complement(2203..4389)
                     /locus_tag="IWO35_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ0317325.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IWO35_002284"
     CDS             complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IWO35_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ0317326.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4611..5051)
                     /locus_tag="IWO35_002285"
     CDS             complement(4611..5051)
                     /locus_tag="IWO35_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ0317327.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5125..5664)
                     /locus_tag="IWO35_002286"
     CDS             complement(5125..5664)
                     /locus_tag="IWO35_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAQ0317328.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6279..7745)
                     /locus_tag="IWO35_002287"
     CDS             complement(6279..7745)
                     /locus_tag="IWO35_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0317329.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2115 a         1721 c         1367 g         2596 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg
     1981 ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca
     2041 tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc
     2101 tgtttttcat gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt
     2161 ttctccatat cgttcccatt catttttact ccatcccttc tcttactttt tcatgtgtcg
     2221 atttaagctc agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc
     2281 aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa
     2341 agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg
     2401 tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc
     2461 cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat
     2521 tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc
     2581 tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc
     2641 aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt
     2701 ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca
     2761 cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc
     2821 taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt
     2881 acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt
     2941 aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt
     3001 acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga
     3061 accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac
     3121 aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt
     3181 tcgtgcacca acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca
     3241 tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt
     3301 gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat
     3361 ccctgagatc cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac
     3421 catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag
     3481 cagaccattc gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg
     3541 aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc
     3601 atctgctgga atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc
     3661 aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga
     3721 catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc
     3781 gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc
     3841 acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa
     3901 tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc
     3961 accgacataa aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa
     4021 gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa
     4081 agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc
     4141 ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc
     4201 tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt
     4261 ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct
     4321 agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc
     4381 tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt
     4441 tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact
     4501 ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga
     4561 ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt
     4621 tgtttacatt cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt
     4681 tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact
     4741 agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc
     4801 gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct
     4861 tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct
     4921 ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca
     4981 agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg
     5041 actttttcca tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca
     5101 gattacgctt gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa
     5161 aaattcttgt ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac
     5221 ttggatcagc tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa
     5281 aaattctaag tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga
     5341 ttttttgttt atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg
     5401 cggcacttta agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt
     5461 ataatttcct tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa
     5521 aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag
     5581 tagcggaaag tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact
     5641 ttgtccaatc acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat
     5701 cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc
     5761 cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca
     5821 aaatatgagg agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt
     5881 acaacctctt ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc
     5941 aacaaacatg agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg
     6001 ctcactacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccagca
     6061 aaacgaaaaa tatgaggggc tatttttgtt tggctgttct tattacttga tgcaggacag
     6121 ctttttcaca acctctgatt cacggtgttc cagaagtagc ttctgcgaaa aacaagccga
     6181 aacttcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac
     6241 cacttctgtc acaacctctt gtaacaagta tttctatttt aaggctgaac cactgtttct
     6301 ggttcagtga tttctttgtt tttgtctcga cgagcaattg ctggcaagat catacctaat
     6361 aagatcaaga caattggtgt gatgatattc gtgaataatt ggaaccacca tgctttagga
     6421 tcagcagtat aatctacttt tggcaccatt cctaggatac atgcaaacgc agtgaaggca
     6481 aagcaccaag caccaaagat aaaaccgatt tttggatttt taacaaattt atattcagaa
     6541 ttgaatttct tataagcttt attcaacatc atatatgcca agaacaccca taggtaacgc
     6601 attggcatga caactgagtt caagttcgtc aaccatttta ctaattcatt catatctttg
     6661 ataccaaaga tcggcaacaa aataatgatt cctactagta tacctgtcag aagataacca
     6721 ttcaccaatg tacctttacg attacgttta cgtagccagt ttggtacaaa ttctggatcc
     6781 gcatctgcta acaagatttt tagtggtgca tcaattgaaa aggctaatgc ggagatttgt
     6841 ccaatcatgt ttgtcaatgc gtaaatgatc atcaatgagt taccgatacc atagtaatta
     6901 cctaaaattt gaaaagcact gtatgcccca tttgccatca agtctttagg aatattatcg
     6961 ctagcaaaca gcatacccat tgcgactgag cctaatacag cacaaatccc taccattgct
     7021 gctaagaaga tcatcccttt aggaaattcc tttgcaggat ttcttgtaga gttgacataa
     7081 ggggatattt tttccgctcc tccaactgca aatactagca tcgaaacggt tgtgaaataa
     7141 ctgaaatcaa atgttggaat atatgttttc acttgattca tatgtggtgt ggcaaattcc
     7201 atcgttggat tgatgaacgg tgccgttaca gctaaaagaa taaataagat cgacatgacg
     7261 aacatcgctg ttccggccag tccgccaatc actttcaaag tagaaagtcc ttttgttgat
     7321 aaatataaaa aggcaagaaa tataataagt gaaataactg ctaccatttg tactgacatc
     7381 gtatttacga tatttccatt tccttggact gcccaaccgc cagcaatcag gattgcttga
     7441 ggcttttgtg ccaaataagg aatatggacg acccagtaag tccaagcggc atagtatgcc
     7501 agtcgtttat tagatgtcgt attttcgacc catgaactga cgcctccttt actatccttg
     7561 aaggctgacc ctaattgtcc aacgatcaat gcgtatggaa taaaataaat ggccaaaatc
     7621 aatagccaag aggtgacaac tgatactcct tgctgagcat agttattaac tacattcccc
     7681 atgccccaaa ccatgttaaa tgcaattaac gcaacagtaa accagcgtaa ttgtttgttg
     7741 ttcattcgtt ctctccttta tgataaaaat ttgttatcca gatgtaatca tattcaatt
//
LOCUS       DACECZ010000113         7789 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.114, whole genome shotgun sequence.
ACCESSION   DACECZ010000113 DACECZ010000000
VERSION     DACECZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.114"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(214..699)
                     /locus_tag="IWO35_002288"
     CDS             complement(214..699)
                     /locus_tag="IWO35_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ0317330.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            1179..1820
                     /locus_tag="IWO35_002289"
     CDS             1179..1820
                     /locus_tag="IWO35_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0317331.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1839..2474
                     /locus_tag="IWO35_002290"
     CDS             1839..2474
                     /locus_tag="IWO35_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0317332.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2492..3307
                     /locus_tag="IWO35_002291"
     CDS             2492..3307
                     /locus_tag="IWO35_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0317333.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3626..5062
                     /locus_tag="IWO35_002292"
     CDS             3626..5062
                     /locus_tag="IWO35_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0317334.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5251..7698
                     /locus_tag="IWO35_002293"
     CDS             5251..7698
                     /locus_tag="IWO35_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0317335.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
BASE COUNT         2606 a         1312 c         1736 g         2135 t
ORIGIN      
        1 tataatcgaa aaaagaagca tttttatata tgcttctttt ttcgtagaat caataaatat
       61 ggatttggct aactatggaa gaatactcgt attagttttc agcgttcata ctagttgtta
      121 cattgtttga ttggccaaaa gaatttctga tatattttgt gctaaataaa gaaaaagacg
      181 cttgaattgg gaaataagaa aactactcta tcattacaaa ttttcataaa ctggcagatg
      241 ttctaagtaa acaagtatct tttcaaagtc ggaaaaatcc ataatcaacg tagtggtatt
      301 gatatttgga tgaaagccta cggtatcttg acgatcaata gcagcatcga tcacgacttg
      361 gatttttttc tctttgtcgt gcatcaatgc taatggagtc actgtacctg ctggaacgcc
      421 taggaggtca ggaagctgtt cttcagaaac aaaagataag cgcttttctt ttaattgttc
      481 agctagttgt tttaaatcgg cgtgcttttc atcaggcaag atgacaagat agatttgttt
      541 tccttttctt gatttcaata atagattttt tacttgcgga ccaggtaggg aaaaagggac
      601 atttttgact gacgtaatcg caggatgatc gactttttga aaagagatac ccagtttttt
      661 tagtagttca taagcttctt gttcggttgc acgactcatc gcgcatcacc ctcctttttt
      721 tattagtgta tcataaaaaa gagttgacaa atgaaaaata atcatgtaaa ttacacgtaa
      781 agttaaaaca aatgacatcg aaaagaagag tagccgttag gtgtttttag agagtctctg
      841 gttggtggaa agaggcagca gttaaccgtg aaacacatct tggagtcgat tttctgaagc
      901 gtatagtagg aaaatccggg gagcccgtta cagctgcggt cgttgctaga tgaccagata
      961 aggctggctt ttccgtgagg agtcggtaaa aaaaggtgga accacgtgat taacgtcctt
     1021 tgatcttcgg atcaaaggac tttttgtttt catctgaacg gcaacgactg tgtgaggttt
     1081 tttctgcaag gaaaaaataa acagaggtgg aaccacgtat aatacgtcct cttggcattt
     1141 gccaagagga tttttttatt ttaaaggagg aaaagaagat ggagtatttg attgaagtta
     1201 tgccgcaatt attagtagga gcactcacaa cattgaaatt atttttattt accttgattg
     1261 gctcattacc attagggatt gttttagcat taggattgtc tttgcatttt aagccgctac
     1321 gctacttgct tcaaacatat gtatgggtta tgcggggaac accgctgttg ttgcaactga
     1381 tttttatttt ttatgggttg ccgacgattg ggatcgtttt tgatcgtttc gaagcagcac
     1441 tcattgcctt tatcttaaac tatgcagctt attttgcaga gatctttcgt ggagggatac
     1501 aatcgattcc tcaaggtcaa tatgaagcag cgcaagtttt gcaattaagt ccctttcaaa
     1561 cggtgacgaa aatcgtcatc ccacaagtga caaagattgt tttaccttct gtagggaatg
     1621 aagttatcaa cttggtcaaa gattcttcct tgatctatat attaggttta ggtgacgtaa
     1681 tgcgtgctgg gaaaatcgcg atggaacgcg acgcgacttt actgcctttg ctaggcgtgg
     1741 caatcatcta tcttacattg actggtattc taacaatcgt aatgaaacaa gttgaaggaa
     1801 aattaaatta ttaccgctaa ctgataggag gaagaaatat gattgaattg aagaaagtga
     1861 acaaagcatt tggtgaaaaa aaaataatca aagatttgaa tctaatcatt ccagatggga
     1921 aaacattagc tatcgtaggt ccttccggtg gcggtaaaac cacgctttta cgtatcttgg
     1981 ctggcttaga aagttctgac agcggagaat tttatttaga cggtgtaccg ttcaacccat
     2041 tcgagcttaa aatgaaagaa caagttgtag gagtcgtatt ccaagacttc cagctatttc
     2101 cacatctgaa tatttttgaa aacattacct tagcaccaaa aatggtattg aaacagacaa
     2161 aagaagaata caccaaaaaa gcagaagaat tagcggcttc attagggatt agtgatttat
     2221 tgaatatcta tccttatcag ctttctggcg gacaaaaaca gcgggtagcg attgccagag
     2281 cattggctat gaatccacgg gtattggctt atgatgaacc aacaagtgct ttagatcctg
     2341 aattacgcca gcaagtcgag cgtttgatcc tatcattgaa agaagatggg gtaacccaaa
     2401 ttattgtcac ccatgattta gtatttgcag aaaatgtcgg tgatcaatta ctaaaagttt
     2461 ccccaattca ttaagaaagg agaagataga gatgaaaaaa accagttttc tattcgcttt
     2521 gatcgcaggt cttcttcttt ttgcaggctg ttcgaacaaa aaaacttcag cagataattg
     2581 ggcgactatc aacgaagaga aaaaaatcgt tataggttta gacgatacat ttgttcctat
     2641 gggatttaga gataaagacg ggaagctcac aggatttgat attgatttag ccaaagcagt
     2701 ctttgaacaa tatgggatta cggtagactt tcagcccatt gactggtcaa tgaaggaatt
     2761 tgaactgaac aatggaacga tcgatttgat atggaacggc tattccaaaa cgccggcaag
     2821 agaaaagaaa gtgcaattta caaaacctta tatggaaaac gatcaagtgc tgatcacacc
     2881 taaagaatca ggaatcacaa actttggaca aatgaataat aagagattag gtgcgcaaaa
     2941 tgggtcatct ggttatgatg tatttacgaa acaaccagaa gtattaaaag agatagtcaa
     3001 aaaccaagat gctgttttgt atgatagttt taatgaagca ttgatcgatc tgaaaagcag
     3061 acgggtcgat ggtctgctga tggataaagt atacgcagac tattatttga aacaaagaaa
     3121 cgaactagaa gacttcaaca taacaaaagg ttcttatgag agtgaggatt ttgcagttgg
     3181 tgcacgcaag tcagatagta cattagtgaa aaaaatcaat gaagccctag atcaactaga
     3241 gaaaacaggt gaattccaaa aaatatccga taaatggttt ggcgaagatg ttacgccgag
     3301 aaattaacaa ataggggact gtgacaaaag acgtgtcaca gtcccttttt ccgaataaac
     3361 agtgttctcc tgccaactcc ttgtgactcg gagctgaatg gcaggctcac aacctcttgg
     3421 taaacggtgt tcaaaagcca gtttttcggt attaagccaa atttatgcaa aaatgtagaa
     3481 agccattttc acaaaatttt tcttactact cgaaactaag cgactttttc acaacctcct
     3541 ttacataatt ttattttgtt gtggaagggg tttcgtgtta ttctaaaaga ggaacgattg
     3601 aaggttatag gaaaggaaga gatgaatgga acacaaagaa cttacctcat ttccaaaaga
     3661 ttttctgtgg ggctctgctt ctgcagcgta tcaagtagaa ggagcttggc aagaagatgg
     3721 gaaaggagaa tcagtctggg atcgatttgt acggatacct ggaaagacat ttaaaggaac
     3781 gaacggtgat ttggcagtag accactatca tcgttataaa gaagatattg ctttaatgaa
     3841 agaacaaggt ctgaaagctt accgcttctc tgtagcttgg acacggattt ttcctaatgg
     3901 acgaggagaa gtcaatcaag ctgggctcgc tttttatgag agattgatcg acgaattgat
     3961 tgaaaacgat attgaaccta tgttgacttt atatcattgg gatcttccac aagcattgca
     4021 agatgaatat aatggttggg aatcacgtca gatcattgaa gatttcactc attatgcaga
     4081 aacattattt gaggcattcc gcgggaaagt ccattactgg attagtttga atgaacaaaa
     4141 tatcttcact tcattaggtt atttgttagc agcacatccg ccaggtgtga cagacccgaa
     4201 acgaatgtat gaagttaacc atattgctaa tttagcaaat gcatctgtca tcaataaatt
     4261 tcacgagtta gaaatgcctg gaaaaatcgg accaagcttt gcgtatacgc caaattatcc
     4321 aattgacagt aatcctgaaa atgttctggc tgctgaaaat gcagaagacc tgatggccaa
     4381 ttattggatg gatgtctata tgtggggaaa atatccgatt gctgccatga ggttcttaga
     4441 agaaaaagga tgggcaccaa cgattgaagc tggcgatgca gaattgctag aatctgctaa
     4501 accagatttt cttgggatca attactatca aacggcgacg aatgcattca atccattaaa
     4561 cggagttggt gcaggaaaaa tgaatacgac aggaaaaaaa ggttcatcag aagaaactgg
     4621 agttcctgga atgtataaaa aagttgagaa tccatttgtc gaacgaacaa attgggactg
     4681 ggagattgac ccagaaggat taaggatcgg actgcgtcgt atcacaagcc gttaccgcat
     4741 gccggtactg attacggaaa atggcttagg agagtatgac aagttgacag aagataaaca
     4801 aatccatgac gactatcgaa tcaattactt acaaagccac gtcaaagcca tcaaagaagc
     4861 aatttcggat ggagcagaag tcctaggcta ttgcacctgg tcttatacag atttgcttag
     4921 ctggctgaac ggttatcaaa agcgatatgg atttgtatat gttgatcaag acgaaacaca
     4981 agaaggatct ttggaacgat ataagaaaga cagttattac tggtaccaaa aactgattga
     5041 agaaaatgga gagaaccttt agttcacata cttaaaacaa gaaggtacgg ctggatatgg
     5101 ccgtgccttt tcttgataaa atcatttaga aattaagaaa aactgtaaaa taagctgaaa
     5161 aatgaaagaa agcgtcagtt ctttcaaaca aaactttagt tcccttactt ttttttgtat
     5221 aatgaaactt gatgaaatga gggataataa atggcacaaa agaaacggcc tactaaaaaa
     5281 aggaaaaaga cgaaaaaaac gaaaaagcaa aaacagcagc aggaacaaat gctgacgatt
     5341 ggtgttgggt ttgccttgat tttatttgct gtcttcggct ttttaaaatt aggtttttta
     5401 ggaatattga ttgcaaacgg ctttcggatc attgcaggaa atacttacca aatcctctgt
     5461 cttttattag ccgtattagg attttggatt gtcattaaga atacagagtt ttcaattggt
     5521 aaaaaccgac gctggtttgg cggcatactc ttttatttcg gtatcttatt attgttacat
     5581 gcccatttat ttggcaaact ccatactggg gaaccaaata tcatggggac gacatgggat
     5641 ttgttagcca gtgatataaa acaaagtcaa gtagacaata acgttggtgg tgggatgatt
     5701 ggagcaatcc tttatcattt tacttacttc ttgattgcac agcctggaag ttatttagca
     5761 gcagtgctat tactggcagg aggagcattc ctgttcagta atctggaagg ctatcagctg
     5821 ttaaatggta tccaatcaat tggagaacga gtacaggaat tattggaagg tgatcctgcc
     5881 aagcaagctc gaaagcaagc ggcaaaggaa gagagaatga aacagcgcgc agaagcgaaa
     5941 gaagcaaggc gcttagctgc aaaagaagct gcagaaaaag aagctgcaga atatgagaag
     6001 aaaaaagcgg tgagccagca aagggaaaat actgctgatg aatggcagga accagaaaat
     6061 acagagccgg agcagctaag ttttgttcca atcgacagtt ttcaagaaaa tatccatcct
     6121 gcgaatctag agaaaccagt accagataca ccaaaacaga ccaatacagc agaaggtttt
     6181 tcagatgagt tgccagagga tgacggtacc tcattagaat ttgagattga agcagaacag
     6241 gaaaatcaag actatgaatt accgacagtt gatttgcttg attctatacc aactgtggat
     6301 caaagtgatg agtataaaaa aattgaaaaa aatatcggcg tattagaaca aactttccaa
     6361 agtttcggtg tcgatgccaa ggtcgtcaaa gccagcttag gaccatccgt cacaaaattc
     6421 gaagtccagc cggctgtagg ggtaaaagta agtaaaattg tgaacttaac agatgatatc
     6481 gcgcttgctt tagcggcgaa agatgtccgg atggaagcac cgattccagg taaatcactg
     6541 attggtattg aagtaccaaa tggaaagatc agtatggttt cctttagaga aatcatcgaa
     6601 gcacaaccaa accacccaga taaattacta gaagtacctt taggacgtga tgtttctgga
     6661 cgggtacaaa cggctgatct ttcgaagatg cctcacttac tcgtggctgg ttcgactggt
     6721 agtgggaagt cagtagcaat taatgggatt atcacaagta tattaatgcg tgctaagccc
     6781 catgaagtaa aactgatgat gatcgatccg aaaatggttg aattgaatat gtacaacggc
     6841 atacctcatc tgttgacgcc ggtcgtgacg aaccctagaa aagcagcgca agccttacaa
     6901 aaagtagtac aagaaatgga agaacgatat gaaaaattcg cggcaaccgg agtaagaaat
     6961 atttctggtt acaatgaatt tgttcaacag aaaaacttgg aaaacggaac gaaacatcca
     7021 accttaccat tcatcgtagt gatcgtagat gaattagctg atttgatgat ggtagccagc
     7081 aatgaagttg aagatgcgat tattcgttta gcgcaaatgg ctcgggcagc ggggattcat
     7141 atgatcctag ctactcaacg tccaagtgtg gatgttatca caggaatcat caaagcaaat
     7201 gtaccttcca gaatggcatt tgcggtatca agcggtacag attctcggac gatcattgat
     7261 tctaatgggg cagaaaagct cttagggcga ggagatatgc tattcttgcc aatgggtgag
     7321 aataaaccga tccgtgtgca aggagcattc atttctgacc acgaagtaga acgggtcgtt
     7381 caatttgtta cagatcagca agaagctcat tatgaagaga aaatgatgcc gacagatgaa
     7441 gttgaaacga caggtgctcc agaacagcca caagatgagc tgtttgaaga agcaaaagca
     7501 cttgtcgttg aaatgcaaac ggcaagtatt tcccttctcc aaagaagatt tcggatcggt
     7561 tacaaccgag cagcacgttt agtagatgaa ctggaagctc atggagttgt tggcccgtca
     7621 gaaggaagca agcctcggaa agtattcatt gagcaggaag aaacatcaga aacgttgccg
     7681 gacgataatg acatgtaaaa cttgtgtgat gaagggttga aaaacgaata accaataaaa
     7741 agacaaggca tgatgatacc aaaagaaaaa atggtttttt tccatcttt
//
LOCUS       DACECZ010000114         7751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.115, whole genome shotgun sequence.
ACCESSION   DACECZ010000114 DACECZ010000000
VERSION     DACECZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.115"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(134..577)
                     /locus_tag="IWO35_002294"
     CDS             complement(134..577)
                     /locus_tag="IWO35_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317336.1"
                     /translation="MEAYEFLGAALRNIFGNLLSACLVAEYLVKRKIVRFNNIFVIMA
                     SIVGIGFAGISKIIGGLMESINDIFAFFGYGAIAILMYQVIPFRKIMPRISRYSYDIY
                     LLHILVFSTIHQIIPNLVLAAFVSIPITLVLSYYYSIMLYRLKLK"
     gene            complement(665..1030)
                     /locus_tag="IWO35_002295"
     CDS             complement(665..1030)
                     /locus_tag="IWO35_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAQ0317337.1"
                     /translation="MYYFQQSYLPAILNKAISIGDTGVHIFFFCSGFGLYLSYLKKPI
                     TLGEFLQKRFIKIYIPYIIVITVSVLLPYMTQDLNAAKVYLSHVLLFKIFLPVYEESL
                     GPFGLFRQLFSSISYSYQL"
     gene            complement(1374..3026)
                     /locus_tag="IWO35_002296"
     CDS             complement(1374..3026)
                     /locus_tag="IWO35_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ0317338.1"
                     /translation="MDHNANYYHWLTNANAEIVEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLSDYLNKTYETPSVVVGYDSRIKSDVFAKVAADIF
                     AANGIQVHLWPELLPVPTVSFAIRYLKASAGVMITASHNPSKYNGYKVYGAGGCQITT
                     EAAAEILAEIEKLDIFADVKMGGDASRIASIQPEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEINEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKNAEGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PTDPVMVKTIVTMDLGERIASNYGVRTVDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLFDKLNEIYAKYGYCLNTLHSF
                     EFEGAAGFTKMQKIMVEFHKGLDMIGGKKVIETLDYSTGLNGLPKSDVLKYLLEGNCS
                     VVVRPSGTEPKLKTYISVSAESKEAAEVMEAVMAAELAEMFK"
     gene            complement(3086..3665)
                     /locus_tag="IWO35_002297"
                     /pseudo
     CDS             complement(3086..3665)
                     /locus_tag="IWO35_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
     gene            complement(4897..5613)
                     /locus_tag="IWO35_002298"
     CDS             complement(4897..5613)
                     /locus_tag="IWO35_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317339.1"
                     /translation="MTVIITMAGLGSRFRKVGYTVPKFMIEAKGKTLFDWSLDSLLDY
                     NSHVEKYVFVVRKEDDATVFIKEHCSLYGIENVEVVEIDYMTDGQATTCMLAIPYCAP
                     DNAIMVYNIDTYVEPGEMKYVDISGEGHIPCFHADGDHWSFAKLNEYGDVVEVREKVR
                     ISNNCTLGAYYFSSAKLYASLYEEYYADERHLEKNEKYIAPLYNYMIEKGMKVTISIV
                     DKSKVHVLGTPEELDVFLNE"
     gene            complement(5638..6036)
                     /locus_tag="IWO35_002299"
     CDS             complement(5638..6036)
                     /locus_tag="IWO35_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ0317340.1"
                     /translation="MFKDYAFVFDIDGTLCPIKKKDEKYEDLVPYANMVEKLRYYKEN
                     GAKITLFTSRNMNSYHGNLGVINANTAKILLAWLDKWEIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLSCTLDQLDEICKKSKLKSE"
     gene            complement(6124..>7751)
                     /locus_tag="IWO35_002300"
     CDS             complement(6124..>7751)
                     /locus_tag="IWO35_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ0317341.1"
                     /translation="PSAKIVPEELHHLGKLPAIIYPINQKIVFDYLYDQYKDVCSAID
                     IACYEKMDKVARRLDKYIKSKTVNIIQLKELGDLGRTIYDSLIGCDEPVIINFADTII
                     NDNIYSLECDSFFYAEDYYSNTWTFFEEKDGDIISVLDKNELKEDDGKKHKLFSGVFQ
                     IMDAQYFRECLRKALMSNVVNVNSFYQALQEYSKRYEFLSIKTNNWFDIGHADKYYNS
                     KLEVKAREFNHISIDKDRSILRKISEDVEKFIGEIKWYLKLPAQVEYVRPRIFEYSTS
                     YINPYVSMEYYSYHTVHELFLYSDLTKKQWIDIFNRIRFVCSDFKRYSVSGDNIQKSL
                     KDMYLDKTFQRFNKLRKDPRFTEFFSSDIQINGVRYKSLDQIEALLSVSVPRELFDIT
                     QFNIIHGDLCFANIMVDNTFSFIKVIDPRGKFGDFDIYGDYRYELAKLFHSVDGKYDF
                     IIKDLFTIKYDPKKAIIDYIVQDRKRDYDLYEVFYSVFKDEIGSDLKKIELIEALLFL
                     SMIPLHGESLNHQMAMLATGLEILGRVVPDIYC"
BASE COUNT         2605 a         1648 c         1138 g         2360 t
ORIGIN      
        1 taatcattta gttttatctc ctttttacca accatctacc actaggttac gaactaccta
       61 agtgcccacc atccgccgcg tggaggctgt taatcaggat ggcaaccgcc cggtagaggc
      121 gacaattatg ctatcatttt aatttaagtc tatataacat aatactataa taatatgaca
      181 acaccaacgt tataggaatg gaaacaaatg ccgccaaaac caagttaggg ataatttgat
      241 gaatcgtact aaatacaaga atatggagta gatatatatc atatgagtat cttgaaatcc
      301 taggcattat ctttctaaat ggaataactt gatacattaa aattgcaatt gcaccgtaac
      361 cgaagaatgc aaaaatatca ttaatagact ccatcagtcc accaataatt ttcgatatac
      421 cagcaaaacc aattccaaca atggacgcca taatcacgaa tatattatta aatcttacta
      481 tttttctttt cactaaatac tctgcaacca aacatgccga aagtaaattc ccaaatatat
      541 tgcgcaaagc agctcccaag aactcgtatg cttccatatc ctgtatgaaa catcataata
      601 gcccagatac taataactca gcttgcaaca agcaatcatt tcattcctat ttttctctat
      661 agttttacaa ttggtatgaa taagaaatag aactgaataa ttgtcgaaat aaaccaaagg
      721 gacccagtga ctcctcatat actggtagaa aaattttaaa taacaaaaca tgagacaaat
      781 atacttttgc agcatttaaa tcttgtgtca tataaggtag taacactgat accgtaatta
      841 cgataatata cggaatataa atcttgataa atcgtttctg taaaaattca cccaatgtaa
      901 taggcttttt taaatatgac agatatagcc caaaacctga gcaaaagaag aatatatgca
      961 ctcctgtatc accaatagat attgctttgt tcagtattgc tggcaaatac gattgctgaa
     1021 aatagtacat caatacaata gcgaatattg aaaacgcctt ctcaaaacta ataatatcac
     1081 gtttatttct ttccccctca ttttattatt tcaatagttt tattcatatt ctactccttt
     1141 catctctatt gctgaataca ttattaaaac tctaatatct cttatttctc ccttactgct
     1201 ttgatcggtg cttcatcagg ctgctgtgtc tctgttaaca cttctttgcc ttttcgatcc
     1261 acacagtcgg cccctttacc tcaattatgt aaacagaacc gtaaagcagg aaatttttat
     1321 taaacataga tgttgctact tcggacattg acactcagat ctttttcata gtattactta
     1381 aacatttcag ccagttcagc agccataaca gcttccatta cttcggcagc ttccttgctt
     1441 tctgcgctaa cgctgatata ggtcttcagc ttcggctcag tgccggaagg ccgaaccact
     1501 acagagcagt tgccctccag cagatatttg agaacatcag acttcggcag gccattcagt
     1561 cccgtgctgt aatccaaggt ttcaatgacc ttcttacctc caatcatatc aagaccttta
     1621 tggaattcaa ccataatttt ctgcatcttg gtaaaacctg ccgcaccctc aaactcaaag
     1681 gaatgcaggg tgttcaaaca gtagccatac ttagcataaa tctcgttcag cttatcaaac
     1741 agactgatac cctgagtctt gtagtaagag aacatctcgc agatcagata agcggcgttg
     1801 acaccatcct tgtcacggac atagctgccg gatagatagc cgtaggattc ctcaaagccg
     1861 aaaatatagc tgtccacctt gcctgccttt tccagcatac caatctgttc gccgatgaac
     1921 ttgaagccgg tcaacacatc gacagtacgc acgccatagt tggaagcgat ccgctcgccc
     1981 agatccatag tcacgatggt cttgaccatc acagggtcag tgggcatctt gccgtgctta
     2041 attctctgag agcatatgta gtccagaagc aacagaccag tttcgttacc ggacagcagg
     2101 acataatccc cttctgcatt cttcacggcg atacccacac ggtcgcagtc agggtcggta
     2161 gccagcagca gatcagcatt atacttcttg gcatactcca tgcccagaga catagcttca
     2221 ttaatttccg gattcggata aggacaggtc gggaaattac cgtctggcag acgctgctct
     2281 tcgacaacgg tcacattgct gtagcccatt tctgccagag tacgggtaac aggcaccaga
     2341 ccggtaccat tcaaaggaga atacacgata gcaacattct tgtccacttc ttcgccaaac
     2401 agaaccgact ggccctttac ttcttcaatg aaagcagtca gaacctctgg ctggatagaa
     2461 gcgattctgg aagcgtcgcc gcccattttc acatctgcaa aaatatccag cttttcaatt
     2521 tcagccagaa tctcagcggc agcctcggtg gtgatctggc agccgcctgc gccatagacc
     2581 ttatatccgt tatacttgga aggattatgg gatgcggtga tcataacacc ggcagaagcc
     2641 ttcagataac ggatagcaaa ggatacggta ggcacaggca gcagctccgg ccacagatgc
     2701 acctgaatac cgtttgcagc aaagatgtcc gcggcaacct ttgcaaatac atcggacttg
     2761 attcgggaat catagcccac gacgacagaa ggggtttcat aggtcttatt cagatagtct
     2821 gacagtccct ggcttgcctt acctacggtg tgtacgttca tacgattagt gcctgcacca
     2881 atggtgccac gcagaccacc ggtaccaaag gacagatttt tatagaagga gtcctcaatg
     2941 tccttctcgg aatatgaccg cagttcctct acaatttctg cattggcatt tgtaagccaa
     3001 tgataataat ttgcattatg atccatagaa tctctccaat cttttaaaat tctgctcatt
     3061 tatttttaag gttaggatga atatatcagc tcaaataata tataacccca ctaatgccgc
     3121 cacccaaagt attataaaat aaatctgaca attggaatgt ctctatgtgt aaaaataact
     3181 gcaaaaactc tataagacac gaacataaaa aaacaaattt tataatctct gataaatttt
     3241 tccaaaatct ttttctgaag ccttatacca cattactaga attataaatg gaataaacag
     3301 aataacattt tcaaaacatt ctgtagttaa tctaatctcc cctgtcgttt cattcacaga
     3361 gtaaatccac caactaccaa acacattaga caaaggattt agccaaacat atctatccat
     3421 cagtgttcta aaaagaatca tatttgtaaa aaacacaagc aaaaacattt ttctaaatga
     3481 gcttgatttt ctgaaatacc gaatccagac aagaaaagat tttttcaaac ctaatcctga
     3541 tttctcctca gccatcgcaa acaagtataa aaacatagcc aaaactgcaa ttaaaatcga
     3601 aacccaaata tttgatacaa tgcagataac acattaacca aaatagaaga tatgattttc
     3661 ttcataaact ctcctttcat catgcgctaa atgagtacca tcaagaaggt ttcgtaaaag
     3721 gaggtaccca ctctaacatg aataacctcg gccagcgaaa ataaaactca acagaattcc
     3781 tatctctatg ctgctataat ttcaacaaat ttacatgtac cagttactaa tctaatataa
     3841 ttttttcagc aaggtaaatt tcacactaca cgcatacaca aaaactgtag aaattaaaat
     3901 tattatcatt ccacaagtca tttctattaa tgaatttgta atccccaatt ttgggaaaat
     3961 aaaatttaaa gcagctatta tatacggatg taaacaaaat aataacgtac taattcttcg
     4021 cagatgtact gttattttta taaatgtact atctttaggc tcagtgatac tgatacaact
     4081 accagtgcta atggaatgga aaatattaac aaattattga cttctcgcaa agctatttga
     4141 tttatgatga aactttcacc aactaataag catcctatag ctaaaacagc acataagata
     4201 aaattacggt atttaaagac cttattaagt atagtgtttt tgtggaaggc taagtgttgc
     4261 ccaataatga cgcatggtac acctataaat aatccattac gagttgttac aaacacgctt
     4321 ttataacaat atctaatata ctcgatggaa tcattcccag ataatataaa ccaataagat
     4381 tcaaatgcta aacctactac gcatagcaac aaaccactga gaaactttat tttagaaacc
     4441 tggcttttgc gtactaacca tagagcaaaa gccagtgcag gcaagaacca caggtgatag
     4501 taactaccaa taaacagaaa atctctgata tacaataata catctgtttt ccagctaaaa
     4561 tctgactgta cccaattctt taatactaat ggaatatata atatagacca aataatgtaa
     4621 attttaagta ctctgtagat ttgtttagat aatttagcgg atgtaacttc accactacac
     4681 aacaaaaagg aactgatgat gaaaaaagta ggaacggcaa ccgtactcag tcctgcaaca
     4741 ataccaatac taaatgctta atctggagct aaatagtttt gacctaaatg acgaacaaca
     4801 actaaaatag ccattagtat ttttagaata tctatcgaat agtactcccc cacacacgca
     4861 gctttataat taacattttc tatgattata ttttccctat tcatttaaga acacatcgag
     4921 ctcttctgga gtaccgagta cgtgaacttt tgatttatct actatactaa tggttacctt
     4981 catgcccttc tctatcatat aattatatag cggcgcaata tatttctcat tcttttccag
     5041 atgcctttca tctgcataat attcttcgta gagtgatgca tataattttg cagaagaaaa
     5101 ataatatgca cctaaagtac aattgttaga aattctaact ttttctctta cttctactac
     5161 atcaccatat tcgttcaact tggcaaaact ccaatgatca ccgtccgcat ggaaacacgg
     5221 aatatgtcct tctccagaaa tatcaacgta tttcatctca cctggctcaa catatgtatc
     5281 tatgttataa accataattg cattatcagg tgcgcaataa ggaattgcaa gcatacatgt
     5341 agtggcttgt ccatcagtca tatagtcaat ttcaactacc tcgacatttt caattccata
     5401 cagactgcaa tgttctttga taaaaacagt agcatcatct tccttacgca caacaaaaac
     5461 atacttttct acatgtgaat tataatcaag caagctgtct aaggaccaat caaataaggt
     5521 tttacctttt gcttcaatca taaatttggg cacagtgtag ccaactttac gaaagcgact
     5581 gccaagacca gccattgtaa taataaccgt catcttattc actttataaa gtctccttta
     5641 ctcagatttc aatttggatt ttttgcaaat ctcatcgagc tgatccaggg tacacgataa
     5701 aaattcatcc ggacgaactg tgcgatcatc aacataaaac cctttatggc ctggccaagg
     5761 ctttccataa agaatttcat catacggaat ctcccactta tctagccaag ccaacaagat
     5821 ttttgcagta tttgcattga tcacacctaa attaccatga taagaattca tattgcgaga
     5881 tgtgaacagt gtaatctttg ctccgttctc cttatagtat cgcaacttct ccaccatatt
     5941 agcatacgga actaaatctt catatttctc atctttcttt ttgatggggc acaaagttcc
     6001 atcaatatca aaaacaaatg cgtaatcttt aaacatataa ctaagacatc tcctttattt
     6061 tagccaggca atttatatgc ccggctttac actgatcaaa tagaaaaaca atagctcaaa
     6121 gcattaacaa taaatatccg ggacaactcg tcctaaaatt tccaaaccag ttgctaacat
     6181 agccatctga tggtttaagc tttctccatg caaaggaatc atacttaaaa acagcaaagc
     6241 ctcaataagt tcaatttttt tcaaatcaga acctatttca tctttgaaga cagagtaaaa
     6301 tacttcatat aaatcataat ctcgtttacg atcttgaaca atatagtcta tgattgcctt
     6361 ctttggatca tattttattg taaacaagtc cttaattata aagtcatatt ttccatcaac
     6421 actatgaaac aattttgcca gttcatatct ataatcacca taaatatcaa aatctccaaa
     6481 cttacctcgc ggatctatga ctttaatgaa cgaaaatgtg ttatctacca taatattggc
     6541 aaagcataag tcaccatgaa taatattaaa ttgcgtaatg tcaaacaatt ctcttggaac
     6601 acttacgctc aataaagctt ctatctggtc caaagattta tatctgactc catttatttg
     6661 aatatcagaa gaaaagaatt ctgtgaaccg ggggtccttt ctgagcttat tgaatctctg
     6721 aaatgtttta tcgagataca tatctttcaa tgacttttga atattatccc ctgaaacaga
     6781 gtatctctta aagtctgagc atacaaatcg tattctatta aagatatcta tccattgttt
     6841 ttttgttaag tcactataga gaaataactc gtgaacagta tgatacgaat agtattccat
     6901 acttacatat ggattaatat acgaagtaga atattcgaat attctaggtc tcacatattc
     6961 tacctgtgct ggcaacttaa gataccactt aatttctccg ataaattttt caacatcttc
     7021 actaattttt ctcaggatgc tacgatcttt atcaattgaa atgtggttaa attctcttgc
     7081 cttaacttcc aatttggaat tataatattt atctgcatgt ccaatgtcaa accaattatt
     7141 tgttttaata gagagaaatt catatcgttt gctatactct tgtaaagctt gataaaaact
     7201 attcacattt actacatttg acatcaaagc tttacgcaaa cactctctaa aatactgggc
     7261 atccataatt tgaaatacac cagaaaacag tttgtgcttc tttccatcat cttcttttaa
     7321 ctcgttctta tctaaaacag aaataatatc tccgtctttt tcttcaaaga atgtccaagt
     7381 attactataa tagtcttcag catagaaaaa gctgtcacat tccaaggaat atatattatc
     7441 attgataatg gtatcagcaa aattaataat aacaggctcg tcacatccta ttagagaatc
     7501 atatatagtt cttccaagat cccctaactc tttaagctga ataatattta ctgttttaga
     7561 tttaatatac ttatctaatc gtcttgctac cttatccatc ttttcgtaac aggcaatatc
     7621 gatagcagaa catacatctt tatattgatc atataaataa tcaaatacaa ttttttgatt
     7681 aatcggatag ataattgcag gaagttttcc aagatggtga agttcttccg gtacaatctt
     7741 agcagatgga a
//
LOCUS       DACECZ010000115         7625 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.116, whole genome shotgun sequence.
ACCESSION   DACECZ010000115 DACECZ010000000
VERSION     DACECZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.116"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(14..706)
                     /locus_tag="IWO35_002301"
     CDS             complement(14..706)
                     /locus_tag="IWO35_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0317342.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IWO35_002302"
                     /pseudo
     CDS             791..967
                     /locus_tag="IWO35_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IWO35_002303"
     CDS             complement(1143..1712)
                     /locus_tag="IWO35_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0317343.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IWO35_002304"
     CDS             complement(1786..2109)
                     /locus_tag="IWO35_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317344.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IWO35_002305"
     CDS             complement(2263..3075)
                     /locus_tag="IWO35_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317345.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IWO35_002306"
     CDS             3429..4277
                     /locus_tag="IWO35_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ0317346.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IWO35_002307"
     CDS             4422..5363
                     /locus_tag="IWO35_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ0317347.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IWO35_002308"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IWO35_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1512 c         1371 g         2470 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacct
//
LOCUS       DACECZ010000116         7601 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.117, whole genome shotgun sequence.
ACCESSION   DACECZ010000116 DACECZ010000000
VERSION     DACECZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.117"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(138..428)
                     /locus_tag="IWO35_002309"
     CDS             complement(138..428)
                     /locus_tag="IWO35_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317348.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="IWO35_002310"
     CDS             complement(546..806)
                     /locus_tag="IWO35_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317349.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="IWO35_002311"
     CDS             complement(943..1356)
                     /locus_tag="IWO35_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ0317350.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="IWO35_002312"
     CDS             complement(1812..2297)
                     /locus_tag="IWO35_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317351.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2681)
                     /locus_tag="IWO35_002313"
     CDS             complement(2436..2681)
                     /locus_tag="IWO35_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ0317352.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2874..3674
                     /locus_tag="IWO35_002314"
     CDS             2874..3674
                     /locus_tag="IWO35_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0317353.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3693..5561
                     /locus_tag="IWO35_002315"
     CDS             3693..5561
                     /locus_tag="IWO35_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0317354.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELCDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5554..6045
                     /locus_tag="IWO35_002316"
     CDS             5554..6045
                     /locus_tag="IWO35_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ0317355.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6033..6587
                     /gene="srlA"
                     /locus_tag="IWO35_002317"
     CDS             6033..6587
                     /gene="srlA"
                     /locus_tag="IWO35_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ0317356.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6605..7600
                     /locus_tag="IWO35_002318"
     CDS             6605..7600
                     /locus_tag="IWO35_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ0317357.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     EKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1445 g         2236 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     2701 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     2761 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     2821 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     2881 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     2941 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     3001 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     3061 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     3121 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     3181 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     3241 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     3301 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     3361 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     3421 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     3481 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     3541 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     3601 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     3661 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     3721 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     3781 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     3841 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     3901 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     3961 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     4021 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     4081 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     4141 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     4201 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     4261 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     4321 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     4381 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     4441 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     4501 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     4561 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     4621 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     4681 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     4741 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     4801 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     4861 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     4921 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     4981 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     5041 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc
     5101 gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt
     5161 atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat gtggatgaag
     5221 aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat
     5281 ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa
     5341 taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac
     5401 ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatgcgat atcctatttg
     5461 aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt
     5521 taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt
     5581 ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc
     5641 aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg
     5701 aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa
     5761 gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg
     5821 ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg
     5881 acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa
     5941 aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa
     6001 agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg
     6061 gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg
     6121 taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag
     6181 accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt
     6241 taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc
     6301 ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca
     6361 atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag
     6421 ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac
     6481 tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac
     6541 agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt
     6601 ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat
     6661 tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc
     6721 aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac
     6781 atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg
     6841 tggaatttat cctaaaaaag ggataccaac catcaatatc gttcccacag gaaaaagtgg
     6901 cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat
     6961 acagccacta atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa
     7021 aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg
     7081 gatagaaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag
     7141 agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt
     7201 gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt
     7261 agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc
     7321 tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga
     7381 gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac
     7441 tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac
     7501 agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt
     7561 tatcattgct tgggtagcaa gttttggatt atatagctga g
//
LOCUS       DACECZ010000117         7596 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.118, whole genome shotgun sequence.
ACCESSION   DACECZ010000117 DACECZ010000000
VERSION     DACECZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.118"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            355..1839
                     /gene="lsa"
                     /locus_tag="IWO35_002319"
     CDS             355..1839
                     /gene="lsa"
                     /locus_tag="IWO35_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0317358.1"
                     /translation="MSLINVSNLTFSYEGSYDNIFENVSFQIDTDWKLGFIGRNGRGK
                     TTFLNLLLGKYAYSGNISSTVKFEYFPYDVEDKSLYTIEVMKSICTEFMDWEIFREIS
                     LLDVQEDALYRPFNTLSNGEQTKVLLAALFLTASCFLLIDEPTNHLDIDARNVVQNYL
                     KRKKGFILVSHDRSLLDQCVDHILSINKTNIEIQKGNFTSWWENKTLQDNFELAENKK
                     LLKEIGRLSYAAKRSSNWSNKVEKSKYGTTNSGSKLDKGYVGHKAAKAMKRAKNIESR
                     HQEAVLQKSELLHNIEQYDDLKISPLEFHKECLIEANDLSLSYGDKEVCSNLNFRVNI
                     GDRVAIIGKNGSGKSSILKLINGDDIKFTGNFMLASGLKISYISQDTSYLKGNLSEFA
                     YNNKIDETLFKTILRKLDFNREQFDKNMVDFSAGQKKKVLIAKSLCESAHLYIWDEPL
                     NYIDIFSRIQIEKMILEYCPTLLFVEHDDAFCNNICTKNINLGL"
     gene            1893..2696
                     /gene="lnu(B)"
                     /locus_tag="IWO35_002320"
     CDS             1893..2696
                     /gene="lnu(B)"
                     /locus_tag="IWO35_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HAQ0317359.1"
                     /translation="MLKQKELIANVKNLTESDERITACMMYGSFTKGEGDQYSDIEFY
                     IFLKDSITSNFDSSNWLFDVAPYLMLYKNEYGTEVVIFDNLIRGEFHFLSEKDMNIIP
                     SFKDSGYIPDTKAMLIYDETGQLENYLSEISGARPNRLTEENANFLLCNFSNLWLMGI
                     NVLKRGEYARSLELLSQLQKNTLQLIRMAEKNADNWLNMSKNLEKEISLENYKKFAKT
                     TARLDKVELFEAYKNSLLLVMDLQSHLIEQYNLKVTHDILERLLNYISE"
     gene            3384..4205
                     /locus_tag="IWO35_002321"
                     /pseudo
     CDS             3384..4205
                     /locus_tag="IWO35_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            4348..4572
                     /locus_tag="IWO35_002322"
     CDS             4348..4572
                     /locus_tag="IWO35_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0317360.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGDEIKISNE"
     gene            4587..5456
                     /locus_tag="IWO35_002323"
     CDS             4587..5456
                     /locus_tag="IWO35_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ0317361.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5437..6171
                     /locus_tag="IWO35_002324"
     CDS             5437..6171
                     /locus_tag="IWO35_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0317362.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6204..7112
                     /locus_tag="IWO35_002325"
     CDS             6204..7112
                     /locus_tag="IWO35_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ0317363.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7109..7357
                     /locus_tag="IWO35_002326"
     CDS             7109..7357
                     /locus_tag="IWO35_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HAQ0317364.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
     gene            complement(7360..>7596)
                     /locus_tag="IWO35_002327"
     CDS             complement(7360..>7596)
                     /locus_tag="IWO35_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ0317365.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFGGTLRYSLGY"
BASE COUNT         2635 a         1094 c         1561 g         2306 t
ORIGIN      
        1 ttttcttgct aattatatga tgcataataa gatatttaaa gaaacggcta tagaaccgtt
       61 tgttttgtta tgcaaattgc aagaaagtta agtattctac actcaataat acattacagt
      121 aaccttatgt tatgtataac aaaaggtaat aatagtattt gtttttgagc cgtaagaatt
      181 tactttcttg cggcttttaa tatttgtaaa agaaaggaag ataaacgtga tagacaatga
      241 tactatgtca ttcatgacaa aatccttgca gagttggata acattataga gtaattaatg
      301 ccgaaaaccg actttgctta taaaattttt ataatgtaaa ggaggaagaa atatatgtcc
      361 ttaataaatg tttcaaatct aactttttca tatgaaggaa gttatgacaa tatttttgaa
      421 aatgtaagtt ttcagataga tacagattgg aaactcggtt ttattggaag aaacggacgc
      481 ggtaaaacta ctttcttaaa tttactgctt ggcaaatatg cgtattccgg caatataagt
      541 tctacagtta agtttgagta ttttccttat gatgtggaag ataagagtct atatacaatt
      601 gaagtaatga agagtatttg tacggaattt atggattggg agatttttcg tgaaatatca
      661 ttgcttgatg ttcaagaaga tgctttatat cgtccgttta atacattgtc aaatggtgag
      721 caaacgaagg tccttcttgc agctttattc cttacagcga gttgtttcct gcttattgat
      781 gaacctacaa accatcttga catcgatgca cgtaatgtag tgcaaaacta tttgaaacgc
      841 aagaaggggt ttattttggt atctcatgat agaagcttac ttgatcaatg tgttgaccat
      901 atactatcta tcaataaaac gaatatcgaa atccaaaagg gaaattttac ttcttggtgg
      961 gagaacaaaa cgttacaaga taattttgaa ctggcagaaa acaagaaact ccttaaagaa
     1021 ataggaaggt tgtcttatgc agcaaaacgt agttcaaact ggtcaaataa agtagaaaaa
     1081 agtaaatatg gaacaacaaa ttctggttca aaactggata agggttatgt tggacataag
     1141 gctgcaaaag cgatgaaacg tgccaaaaat attgagtcaa gacatcagga agccgtttta
     1201 caaaaatcag aactgctcca caacattgaa caatatgatg acttaaaaat ttcaccactt
     1261 gaatttcaca aagagtgctt aatagaagcg aatgatttat cattgtctta tggagataaa
     1321 gaagtatgca gtaatcttaa tttcagagtc aatattggtg atagagttgc cattatcgga
     1381 aaaaatggga gtggtaagtc tagtatccta aaattgatta atggagatga tattaaattt
     1441 accggaaatt ttatgctagc aagtggacta aaaatttctt atatttcgca agatacttca
     1501 tatttaaaag gtaatctatc tgaatttgcc tataataata agatcgatga aactctattt
     1561 aaaacgattc ttcgtaaact ggattttaat agagagcagt ttgataagaa catggtggat
     1621 tttagtgctg gtcagaaaaa gaaagtacta attgctaaaa gcctttgtga aagtgcacat
     1681 ttgtatatat gggatgagcc attgaactat attgatattt tttcacgtat ccaaattgaa
     1741 aaaatgattt tggaatattg tcctacacta ttgtttgtgg agcatgatga tgctttttgc
     1801 aataacattt gtacgaaaaa tattaattta ggtttgtaga gattttgagt tgccacaata
     1861 actaaaagat atttacatga aagggtgaag aaatgttaaa acaaaaagaa ttaattgcaa
     1921 acgttaagaa tcttactgag tcagatgaac gaattacagc ttgtatgatg tatggatcgt
     1981 ttaccaaagg agaaggtgac caatactctg atatagagtt ctatatattt ttgaaagata
     2041 gtataacctc gaactttgat tcatccaact ggttgtttga cgtagctccg tacttgatgc
     2101 tttataaaaa tgagtacgga acagaggtag ttatttttga taatcttata cgtggggaat
     2161 ttcatttcct ttctgaaaaa gatatgaaca taatcccctc gtttaaagat tcaggttata
     2221 ttcctgatac gaaggctatg cttatttacg atgaaacagg gcaattagaa aattatttat
     2281 cagagataag tggtgcaaga ccaaatagac ttactgaaga aaatgctaat tttttgttgt
     2341 gtaatttctc taatctatgg ttgatgggaa tcaacgttct aaaaagagga gaatatgctc
     2401 gttcattaga actcttatca caacttcaaa aaaatacact acaacttata cgtatggcag
     2461 aaaaaaatgc tgataattgg ctaaacatga gtaaaaacct tgaaaaagaa attagccttg
     2521 aaaattataa aaaatttgca aagaccactg ctcgattaga taaggtagaa ttatttgaag
     2581 cctataaaaa ttctttgcta ttagttatgg atttgcaaag tcaccttatt gaacaataca
     2641 acttaaaagt tacacatgac attttagaaa gattgttgaa ttacattagt gaataggaga
     2701 gtaacgttat tcacagtatt gggtactgaa aattaacagc atacctcagt ttttcaaaaa
     2761 acagcgaagc ctatggatat gttcccgtct accgatagtg caaaaagtgt cgtggatatg
     2821 tttccgagaa cggacataca caaatgacat tcattctgtc ctctcaatcc atgtggaagc
     2881 agtatgtctg ttaacaagaa aataattatt gttcgtcctg acgagttgtt agactcgaaa
     2941 aggataaaca tcatttttag aaccggtagc cgatttttgg ctagcggttt tttgggctct
     3001 ttggcaaata gtggtgattg aatctcgcgc tatcctatgg aaaaccgcct tgtgtgaggg
     3061 gctgcgagac cgcttcgatc ctagaattgc tgaagcacga atctagcgtc actcacattt
     3121 catcttttga cgtagctgag acaaggtgtt tttcttcgat ccgctagtgg ttaatctctt
     3181 tttggtgcaa atatggttta gaatttcttt gcatggctta agggagtgga gaatcggata
     3241 ttagtaggat ttacatccac agataaaaat aggcttgatt ttaacagcga aactaaaata
     3301 ccctacttaa atgaagtgat agattagatg ttgggttgtt aggagatgct taaaaatcct
     3361 atgcaatcaa tagtttttag tgtttgttga cttgatccct aaatcgcctg agtactaaac
     3421 gttgagtgtg tctgcttact gtcactagcc agtaagtgag actgtagtta atctctcatt
     3481 ttccccagta ttatgttgaa aatagccatg aagcgattat ttctaaagag ttatttttgc
     3541 aggctcaaga agaaatttat cggagaagta atatctacac aggagaaggc aagaacaaaa
     3601 gaatttatag tagcaagtac gctttaagtg ccatcacctt ctgtggatat tgtggcgata
     3661 tttataggag aacctattgg aatattcatg gcagaaaaga atttgtctgg cgatgcgtga
     3721 ctagaatcga gcagggtccc gaggtatgta agaaccgaac cgtaaaagaa gatgaactct
     3781 atggtgcggt aatgaccgca attaataagc tacttgctgg tggcaacaat atgataaaga
     3841 cactggaaga aaatattcat gctgtgattg gagaaaccac agaataccaa atttcagaga
     3901 ttaataccct actagatgaa aaacaaaaag aactaatcaa gctggccaat aaaggtcaag
     3961 attatgaata cctggtagat gagatcgatg agatgcgaga taagcgtcag actcttttag
     4021 tagaagatgc ctccctcagt ggcgagaacg agcgcatcaa tgagttgatt gaatttatcc
     4081 gcaagaacaa attccgcacc ttagaatatg atgataagct tgtaaggaag ataatccaga
     4141 acgttaaggt ctatgaagac cacttcgtca tagccttcaa accaggtatc gaaatggaaa
     4201 tatgaaaacc agaagtaaat agcccatgac tctgaagtag agttgtgggt tttcttttaa
     4261 ttgctgatta taataatatt ttaatgacta ttgcatttat caaccaaatg gtttatgtta
     4321 ttcttagcat aaacttggag gtgctgtatg actacggcag aaatgattaa agaactgtgt
     4381 gagcaaatga atataagtgt ttccgaactt gctagacgta ttggccagac tccacagaat
     4441 tttaataaaa aattacaacg agaaacagta accttggatg agttgaaggc cattgctgat
     4501 gtgctaggtg tcaagtttgt gcaggcattt attttaccag atggagatga aataaaaata
     4561 tctaacgaat aaaggaggat gtttatatgg taggtaatgt tattcaaata gtaacagaga
     4621 aattatcatc tttgcctttt atagaaggca ttgtattagg gggctcacgt gcaagaagca
     4681 cccatacaga aaattctgat atagatatcg gaatctatta caattcagac tcatttgacc
     4741 tgacagcgat taatcaaatt gctacagagt tggatgatga gaatagaaac aaccttgttg
     4801 tacctcccgg agcgtggggt gattgggtta atggcggtgg atggttagtt ataaacgggt
     4861 gtcatgttga cttgatatta cgtgatataa aacgggtgga acaaataatc aaagatacgg
     4921 agcaaggaat tgttactgcc aattatcaga ccgggcatcc ccatggttat ataagtgcta
     4981 tgtatcgtgg agaattagcg attagtaaaa tactatatgc taagaacgaa agcttatgcg
     5041 aattaaaaaa gcaggcagaa atttacccta ctgctctaaa gaaaagcttg atgaactttt
     5101 ttatatttga agctgaattc tctttaatgt ttgtaaaagc aaatgcgggg gcagaggata
     5161 aatattatat tgcaggccat gtttttcgta ttatttcatg cttaaatcaa gtactatttg
     5221 catgtaataa tgcttattgc attaacgaaa agaaagctat aaaactgctt gaaacttttg
     5281 aatataaacc tgaaaaatat gccgaaagag ttaatcatat ttttgaagta ctcggtcttt
     5341 ctctttttga atgctacgac atgaccgaga aactttataa agaagtgaat gaaattgtat
     5401 cggagataaa taacttttta aacgaggaga gttcagatga aagaaaacaa atatgatgat
     5461 aatatatttt ttcaaaaata cagtcaaatg agtcgctcgc agaaaggact ggctggtgcg
     5521 ggagaatggg agactttgaa aaagatgcta cctgatttta agggtaagcg tgtgcttgat
     5581 ttaggatgcg gctatggatg gcactgtata tatgcgatgg aaaacggtgc ttcctctgta
     5641 gtaggtgttg atatttctca taaaatgctc gaagtagcaa aaggaaaaac ccattttcca
     5701 cagattgaat atgaatgctg tgccatagaa gatgtggatt tcccagagga gagctttgat
     5761 gtaatactaa gttcgcttgc gtttcattat gtagcagact atgagaattt aataaaaaag
     5821 atatatagga tgctgaaggc tggtggcaat ttagttttta cagttgaaca tcctgttttt
     5881 actgctcatg gaacacaaga ctggtattat aacgaaaaag gagaaatact gcatttcccg
     5941 gtggacaatt attattatga gggcaaacgg acagctatgt ttttggaaga aaaggttaca
     6001 aaatatcata gaacactgac cacatatcta aatacactgc tttcaaatag ttttataata
     6061 aatcagattg tggagccaca gccgccagag aacatgatgg atattccggg gatggcggat
     6121 gaaatgcgac gcccaatgat gctgattgta tcggcaaaaa agaagatgta ataatataga
     6181 aaaaataaac gaggagtatg taaatgagat cagaaaaaga aatgatggat ttagtacttt
     6241 ctttagcaga acaggatgaa cgtattcgaa ttgtgaccct tgaggggtca cgcgcaaata
     6301 ttaatatacc taaagatgaa tttcaggatt atgatattac atattttgta agtgatatag
     6361 aaccgtttat atctaatgat gactggctta atcaatttgg gaatataata atgatgcaaa
     6421 agccggagga tatggaatta ttcccacctg aagaaaaggg attttcctat cttatgctat
     6481 ttgatgatta caataaaatt gatcttacct tattgccctt ggaagagtta gataattacc
     6541 taaagggcga taaattaata aaggttctaa ttgataaaga ttgtagaatt aaaagggaca
     6601 tagttccgac tgatatagat tatcatgtaa gaaagccaag cgcaagggag tatgatgatt
     6661 gctgcaatga attttggaat gtaacacctt atgttattaa aggattgtgc cgtaaggaaa
     6721 ttttatttgc tattgatcat tttaatcaga ttgttcgcca tgagctgctg agaatgatat
     6781 catggaaggt cggcatcgaa acaggcttta aattaagtgt aggcaagaac tataagttta
     6841 ttgaaaggta tatatccgag gatttgtggg agaaactttt gtccacctac cggatggatt
     6901 cctatgaaaa catatgggaa gcattatttc tatgccatca attgttcagg gcggtatccg
     6961 gtgaggtggc ggaaaggctt cattatgcct atccggagta tgataggaat ataacaaaat
     7021 ataccaggga catgtataaa aaatacactg gtaaaaccgg ctgcctggat agcacatatg
     7081 ccgctgatat agaagagagg cgggaacagt gattacagaa atgaaagcag ggcacctgaa
     7141 agatatcgat aaacccagcg aaccatttga ggtgataggt aagattatac cgaggtatga
     7201 aaacgagaat tggaccttta cagaattact ctatgaagcg ccatatttaa aaagctacca
     7261 agacgaagag gatgaagagg atgaggaggc agattgcctt gaatatattg acaatactga
     7321 taagataata tatcttttaa cccgtggtgc tagttaattt taatatccta aagaatatct
     7381 caaagttcct ccaaaacgtc gttgtgctcg acttaataga acactgtaag ccagtaatat
     7441 gctttcaaat ctactctcta gccctttaac agaacgtgag ttgaaatttt gacaacctaa
     7501 tttttctaag gaagaaaaaa cagtctcaac tgtttttctt tgttttccaa gtaagctgtg
     7561 gtctagtttt tctgatttat ccatgttttt acgtgg
//
LOCUS       DACECZ010000118         7397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.120, whole genome shotgun sequence.
ACCESSION   DACECZ010000118 DACECZ010000000
VERSION     DACECZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.120"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..142)
                     /locus_tag="IWO35_002328"
     CDS             complement(<1..142)
                     /locus_tag="IWO35_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0317366.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(183..437)
                     /locus_tag="IWO35_002329"
     CDS             complement(183..437)
                     /locus_tag="IWO35_002329"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0317367.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(548..802)
                     /locus_tag="IWO35_002330"
     CDS             complement(548..802)
                     /locus_tag="IWO35_002330"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0317368.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(995..1270)
                     /locus_tag="IWO35_002331"
     CDS             complement(995..1270)
                     /locus_tag="IWO35_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAQ0317369.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1242..2195)
                     /locus_tag="IWO35_002332"
     CDS             complement(1242..2195)
                     /locus_tag="IWO35_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0317370.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2807..4300
                     /locus_tag="IWO35_002333"
     CDS             2807..4300
                     /locus_tag="IWO35_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAQ0317371.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4435..4731
                     /locus_tag="IWO35_002334"
     CDS             4435..4731
                     /locus_tag="IWO35_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ0317372.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4755..5714)
                     /locus_tag="IWO35_002335"
     CDS             complement(4755..5714)
                     /locus_tag="IWO35_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0317373.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5897..6748
                     /locus_tag="IWO35_002336"
     CDS             5897..6748
                     /locus_tag="IWO35_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317374.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            6769..6981
                     /locus_tag="IWO35_002337"
     CDS             6769..6981
                     /locus_tag="IWO35_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317375.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         2442 a         1199 c         1330 g         2426 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga
      241 ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca
      301 tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca
      361 tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg
      421 atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct
      481 aataaccgac acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca
      541 agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct
      601 catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg
      661 actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt
      721 ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaacaatc atttgtttaa
      781 aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct
      841 tgtctctacg aaaagctgtc taagatttga tactaacacc aaatgttgta tacccccaaa
      901 caatacacat attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa
      961 atatatttac tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata
     1021 taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt
     1081 catacatagg aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat
     1141 ctacttgaac tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa
     1201 tatcatcttt aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta
     1261 ataatgccat ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca
     1321 gcatatttag gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt
     1381 ctttcttcta attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa
     1441 tcatttaatt ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg
     1501 cttttaaaaa tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga
     1561 gaggaacgtc gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct
     1621 tcaaaattcg tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt
     1681 gcgtaccgtt ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga
     1741 accacattat ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat
     1801 tcttctaaag gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac
     1861 aagtattcat taagttcttc agatccagga gagccagcca taatatataa atgtggactt
     1921 acttgagtaa tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca
     1981 aagctagtaa tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa
     2041 tcaattagca acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg
     2101 tctgtagtct taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta
     2161 tctgatgttt taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa
     2221 atatatttag tatacttcat atacttaata tattcgatat atctaatata ctccaaattg
     2281 ataagaattc agactttata aggaatatag tggatatact aaatatattt gctatattca
     2341 gtatatcaga tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag
     2401 agtgaaaaga ggtaaactta attctataga aaccacagat aattcactat ttacaaagta
     2461 gcaaaagcag tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat
     2521 tgattttagc agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt
     2581 ctcaagaata tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc
     2641 cgattgcctt tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt
     2701 gtacttaaat taaatattct taaacactga agagagaaca catactatgg ctgagattaa
     2761 agctaaactg tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat
     2821 ttattcagct attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa
     2881 acctgcaggt catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca
     2941 attaatgaat gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga
     3001 aaagaaacta acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta
     3061 tcgaacagtg gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt
     3121 tgatatcgat tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt
     3181 gggctacatg ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact
     3241 aaagacacca ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa
     3301 aatttcaaaa acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg
     3361 taaccacttc ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa
     3421 ctatcagtat tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa
     3481 aacaaaagaa gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca
     3541 agtagatgaa ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg
     3601 acgtcttggt cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta
     3661 tgcattggat acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct
     3721 atctgaagca gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc
     3781 aagcagagaa ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa
     3841 tgatctgttc attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa
     3901 atatagtcat aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct
     3961 tgctagtgag ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc
     4021 caagcgaaca cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa
     4081 ggtaacacga ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag
     4141 ggttattcaa ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt
     4201 tggactcaac gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc
     4261 tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg
     4321 ccttatacaa tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt
     4381 aggtggtata cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg
     4441 ttaaaaaatt atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta
     4501 tctgtggaag tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt
     4561 accagggaaa aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct
     4621 tctggggaga ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt
     4681 gagtataaaa tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta
     4741 tttactctta attgtcaaat taagttagac aaatcatcaa tacttacgta actcaaaaat
     4801 acttccaaag gtgttcgata attcagtgat tttctaggaa tattatttcg tttcgatgcg
     4861 acagattgaa taaaagattc atctactgaa ttgaaatcca tagatttcaa taaaccatct
     4921 ctacgtaaca atccgttaga attctcgttt aggcctcttt gagacggcgt tcctggatca
     4981 gcgaaataaa tggcaatatc attggcatta ctgatctgtt tccaatttga aaattccttt
     5041 ccacaatcaa aagtgatgga tttgaataga tttttcggta cagattcaaa ccaatgattt
     5101 aatttgtttt caatatcaat cgcttgtcta ccacacggtt tcaatgtgat gataactttt
     5161 gataatcgct caactaaggt aattacagca cttttatgtt tcaaacctac gatagtgtca
     5221 ccttcaaggt gaccaaactc atttgagaat tggctataat ctttttcacg ttcatgaata
     5281 gagcgacgga aagcttgttt tccacgtctt tcttgatgtc catttggttt acgcttgcct
     5341 ttcatcggta agtcagaagg attaaatagc ccctttttga acatacgata aatggtacga
     5401 gcagaacaac gaataggaaa cgctgcacga ccaacaatca catcgggtgt ccatccttga
     5461 acaacctttc tttgaatata ttctgattgt tcctcaggta aaacaagcgg gcgtctacca
     5521 cagtttgatt tgtttttctt atactgttga tagtactcta aggctgattt tccttgcttg
     5581 aagaacaggt atactttatg gatcgtttgt cttgaacgat tcaagaaatg cgcagttttc
     5641 gcaacagatt gatttatttt gaaataagac tctatcatta caagttcatc cgttgtaaga
     5701 tgggtatagg tcatttgtac tcactcctta taattctttg gacggaacta ttttgagtgt
     5761 agcacaaatg acttttttag ttgtctagct taattttaca atcggcgtac ttaaaaaatt
     5821 ggttcattga gcaacctatc gctgctaaag tagcaaagga aaacgttaaa ggaaagaatt
     5881 tagggaagaa atcgaaatga aatcggacga ctggattgaa cacacctggc ttcctctgaa
     5941 aagaaatagc aaacgaaaga aattttgggt attgcaatta tggccattag gaaaactttg
     6001 tagttacggg gcacgactta ttcttgattt aaccgtttac tcgattggag tatttggaac
     6061 ggcttattac ttgataaatg atcccaatag gattgcagca tcagacattt ttacagtgct
     6121 aatgacgttg atttttgtca tgctattgtc tatttttact caattagtca tgcaatttac
     6181 caggcaaaaa ttaacagcag gaaaactttt caaaagccta ttcgatttaa ttgttgtaaa
     6241 tgcaattcta gcgactttaa taaaacaaat tgaacaatct ggaagaacaa caaaaggagt
     6301 gaaggatttt ttattgacgt ttattcactt tgtaaatgga cacatgtatt tgtttgaagt
     6361 gggtattatt ctcttttttg tcgggtatgt atccacgaaa cttattaaac gttcaattga
     6421 aaaagaaatt gaaattgacc tgattccgac acttcttggt tatgagtaca atgttgattt
     6481 tggaagatat gacgaattga tcaatacgga tatcgttcac acgagtatcc atgaatctac
     6541 tgttaagggc atcagatcta ttttaaaaaa tggccaggaa atgaaagcaa tagatcgaag
     6601 aaaagagatc tacaattttt atgaagtcga agcaagccta cgatatggca tcggaaaatt
     6661 gtggttagat ggaagtaata ttcgtcaaaa caaaccatac attttttgga acagtagtga
     6721 tttagaactt ccaatgaaag ataaataatt atggagaatc gtttgaaaat gattcctcta
     6781 tacaggattg tgcttatagt tggcatggag ttaaaaacct taaagaagga gcagttattc
     6841 aaagtgaaag aacgtcataa tcagttacta tgtagtaaaa cgggtacgat gaacattgaa
     6901 taccgaacag aatatttaga attaacgaat caaactgatt ttttgttgat tcgaaagcaa
     6961 caagagcaag aaaaaaatta atggctactt atagccaatt gattgatgaa tgggaacaat
     7021 tcaaggaatc taaaattcca gaacgtctct aaaagccttt atttgtaaag agcataagga
     7081 aacgtgggaa cactttttaa aaaaagtgtt ccaataggct gtgcctatta cgtttctcaa
     7141 aaataaatgt gggaacaaaa tatttaaaaa taggatagtg aaaggatagt gaaggatagt
     7201 aaaaggaggg tagtaggata gcaaaaagga taggctcctt ttcgctatcc tttttaaatt
     7261 ggaggaatta atatggaagt aagaataaga aatgtggatg agaaaatcgt aagagaaatt
     7321 gatcgaagat gtgacgaacg gttctgttgc aaagttttaa atctactatc aaataaggta
     7381 gaataataga aaaagat
//
LOCUS       DACECZ010000119         7323 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.121, whole genome shotgun sequence.
ACCESSION   DACECZ010000119 DACECZ010000000
VERSION     DACECZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.121"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..656
                     /locus_tag="IWO35_002338"
     CDS             <1..656
                     /locus_tag="IWO35_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0317376.1"
                     /translation="LLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYA
                     FLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGID
                     PTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGA
                     LYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            668..1588
                     /locus_tag="IWO35_002339"
     CDS             668..1588
                     /locus_tag="IWO35_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0317377.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1629..3086
                     /locus_tag="IWO35_002340"
     CDS             1629..3086
                     /locus_tag="IWO35_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0317378.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3431..4045
                     /locus_tag="IWO35_002341"
     CDS             3431..4045
                     /locus_tag="IWO35_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0317379.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4038..5762
                     /locus_tag="IWO35_002342"
     CDS             4038..5762
                     /locus_tag="IWO35_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0317380.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5759..7210
                     /locus_tag="IWO35_002343"
     CDS             5759..7210
                     /locus_tag="IWO35_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0317381.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2501 a         1219 c         1465 g         2138 t
ORIGIN      
        1 atctgctgat ctcagtcgta tttgcaatca ttatgagcga gttgagaaat aaacgactcg
       61 ttaaagttta tcaaacgatg tccttactac cttattttct atcttgggtc attatcagtt
      121 actttgttta cgctttttta agtcctgaca aaggaatatt caatcagtgg atcactggac
      181 atggaggaga agctgtcaat tggtataatg aaccgaaata ttggccgttt atcttggtgt
      241 tcattggtac atggaaaggg attggttata acagcattat ttactttgca tctgttatgg
      301 gaatcgatcc gacttattat gaagctgcta tggttgatgg agctagcaaa tggcagcaga
      361 tcaaacatgt gacgatacca caacttattc cactaatgac gattttaacg attttagccg
      421 taggaaacat tttccgagct gattttggcc tgttttataa cattcctagg aattccggtg
      481 cattatatga tgtgactcaa gttttggata cgtatatcta caatggcctg acatccacag
      541 gagattttgg tatgacggct gctgcaggac tttaccaatc agttgtcggc tttgtcttgt
      601 tgatgatcac aaatacgatc gcccgacgtt ttgatagcga ttctgcatta ttctaaggag
      661 gaaaaaaatg aaaaaaaaga aagtggagag agtccaagtc cgctctttca ataagacggc
      721 taattttttc tctaatataa tgattgcact ctttgctatt tcctgtattt tgccatttat
      781 atttgttatc gccatctctt ttaccgatga gtccatattg tcgactcacg ggtacagttt
      841 ttggccagaa aagttcagta tatttggtta tacgtacttg ttcgaccaga tgcaggataa
      901 aattttccaa gcgcttttcg tcactgtact tgttactgtt ttaggtacac tgatcaatag
      961 cacggctact tctttatatg cttatgcgat ttcacggtca aactttccat tccgtcgttt
     1021 ttttaccgtt ttttgtttga ttacgatgtt gttctcacca gggatggtgg ccaattatct
     1081 tgttatgaca aatcttcttc agttaaaaga tacgatttgg gcattgatct tgccaatggc
     1141 tgtcagtccc ttcaatatta ttgtgatgcg cacattcttt aaacgttcgg tctctgattc
     1201 gattattgaa tcggcacgga tcgatggagc tagcgaactg cgaatatttg ttcagatcgt
     1261 tttaccgtta gctgttccag gtattgcgac aatcagttta tttgccgcat tgggttactg
     1321 gaatgactgg ttcaatgctt tactatacat ccaagatgat aagctagttc cactacagta
     1381 tcttttgatg aaaatccaaa gtaatattca atatctgaca caaaattcag gagcaggaag
     1441 ccaattgaca ggcggattag cctcagttcc aggagaatct gccagaatgg cgatcgtagt
     1501 catctcgacg ttgcctattg cgatcagcta tccatttttc cagaagcatt ttgtcaaagg
     1561 tctaacaatc ggcggagtaa aagaataaag aaacaaaaat ataaatcata aaattaggag
     1621 gaagaaaaat gaagttatgg aagaaactag cctttacagg tgtttcagcg ttgttgttgg
     1681 gaacattagc agcatgcgga ggttctggtt ctaaagacca ggcagaagct agcaattcgg
     1741 acactttgca gatgtatcaa atcggagata aaccagataa tttcgatcaa ttgatggaag
     1801 tagcaaacaa acgaatcgaa aaagagatcg gtgtaaaagt caatatcaat tacattggct
     1861 ggggcgatta cgaaaagaaa atgaacgtca tcatttcttc cggggaaaat tatgatatcg
     1921 cctttgcgaa caactatgtt tccaatgccc aaaaaggagc ctttgctgat ttgactgaat
     1981 tagcgcctaa gtacgctgaa aaagcttata atgatttaga tgaagcatat attaaaggaa
     2041 atctggtcaa tggaaaatta tatgctttcc cagtcaatgg aaatgtcttt gctcaacaag
     2101 tacttacttt taacaaagct ttactagata aatacgatct ttcaattgat ggaatccaat
     2161 cttacgctga tgcagaaaaa gtcttgaagg aattccatga aaaagaacca aacacggcag
     2221 cttttgccat tggacaaggc ttcaaagtcc aagggaactt tgattatcct ttagggaata
     2281 cgttaccatt tgctattgat ctggatggag atgattcgaa aatcatcaac caatacgata
     2341 atgaaacgta tatcggactg ttacgtacaa tgcatagctg gtacaaacaa ggactcattc
     2401 caagtgacgc tgcaacgagc aataaggatt atccactaga aggaaatact tggctaatgc
     2461 gtggagaaac acaaggaccg tatgattatg gcgatacaat tttgacaaat gcagcaaaac
     2521 aagaacttgt ctcaaaagca attactgttc cactaaaatc tacagctcaa gcacaaatgg
     2581 caaattttgt cgtatcaaat acttcaaaaa ataaagaaaa atcagtagaa cttctagggc
     2641 tgcttaacag tgatccagaa ttattgaatg ggcttgtttg ggggatcgag ggagaagcat
     2701 gggaaaaagt cgatggttca gatgataaaa tcaaattgct agatggttat cagccaaata
     2761 cacatatgtc tgcttggaat acaggaaaca acaagattct atatacacaa gagtctatta
     2821 cggatgacat gattgccaaa cgtgatcaat cgattgctga tgcagaaact tcaccgattt
     2881 taggattcag cttcaacact gacagtgtca aaacagaatt aagtaatatt tctaatgtca
     2941 tgaatcaata tttagatgga ttgaatacag ggactgttga tccagatgaa acattaccga
     3001 aattgaaaga cgctttggac aaggctggct atgataaggt attgaaagaa atgcaaaaac
     3061 aatatgatga gttccgacaa gaataataat gaataaagac aaaggggttg tgacaaaaga
     3121 cgtgtcacaa cctcttgttc cgaataaacg gtgttcaaaa gccagtttct cggtattaag
     3181 ctaaatatat gtaaaaatgt ggaaagccat attcacaaaa tttttcttac tactcgaaac
     3241 taagcggcta tttcacaaac tcttggtaaa cggtgttcaa tcagctaacc ctcggtattc
     3301 gctcacaaaa cgacaaaata tgaggaacta tttttccgtt tgtttctcta ataatcaggt
     3361 caaggtgctg attgcccaac ctttttttgt ataatgagaa agtataaact taatgtttgg
     3421 aggagaagat atgttaggga aggttttgga agagttattt atccggatct gggtcattat
     3481 aaaactaacc ttgtatttct ggatatatac atttgccggc gggatcatct ttggattagg
     3541 tgcggcatgg aaaacagtca atgaactatt ttatttgtac ggattcgagt ataaagaaat
     3601 cacaataaaa agaggctgga atatatataa aagaaatttt ttaagaggca atctgctatt
     3661 tagtttattt ttatctggta ctgctttatt gagttacaat ttatatttat ctgttcaaat
     3721 caaagggctc atttttttag ctattgattt tattttactt tttgccttgc tctgcctatt
     3781 tgctacttat caatacagta tgattttaga tagtgaatat acgatctctg ttcctaatct
     3841 gctaaagcta agttttattt cggttttttc cagcttttcg tcttttctaa aaataattat
     3901 tggtagcggt ataattttag gcgtgacttg gcaattcaaa ggattgatcc tgtttggtgt
     3961 cattggtctt ttgacggttt ggaatggtac gatgacaaca cattggcgtg aagaattaga
     4021 taaacagttg gaaagttatg aataagtttc ggattctatt ttcaaaagga accttactaa
     4081 atcgtctgtt gcggaaatac gcggttatca tgatcggtgt aacgatgact gctaccgtta
     4141 tttttagtat ccatacttgg gaacaaaatc aaaaacaggc agaaaacatg acaagtgatg
     4201 ccgttcaaag cacaagcaga atgctgaatg ataaaacaac cttgagtagg atcatcaaaa
     4261 accagcttgt agggaattcc gaaaagatcg aaaatgtgac gacctactta acaaaaccca
     4321 tagatcagta tctgatgtat gtatacgaac agcaaaacag taccgatgaa ttagtttctt
     4381 tcccaaatca aatcaaagat ctctacgcaa attatgaaga actaagtgcg atatatatcg
     4441 tgttgaatca actaccggag tattatgaat cttctagaga aaacaaaggt ggggaaataa
     4501 aaaaggggac accaaaatta ctagacgcct tttatatcaa gttgcctatc acacaaggag
     4561 gagctgagtc aggaagtatt tttattggtt ttcaaaagaa agaactagat atgattttag
     4621 aaaatctaac gtcattcaat ggactctctt tatacatgat ttctggaacg acaaatcgac
     4681 tatacacatt ccatgataat aaaatagcta aagaaacgtt tgaaagacaa gaaatgatca
     4741 tcacagagag tctgaagaaa aattcacaat tgccgattga cgatctggaa aaaaacaatt
     4801 ttgttcaaca ccaagaactg tctgatgatt atcgtatatt agctgtacta gataaaaaaa
     4861 gtgtaaaaat cgaaacggga aaaaatcttg ccccgctgat cattggtatc attattttag
     4921 acaatatact gcttatcttt ctttataaaa cattcaaacg atattcacag caagtatcta
     4981 tcgtcatgca ggccatggat caaacagcag cagggaattt agagacgcgg atcgacataa
     5041 caaacacgga atatgaactg aaggaacttt caatcggtat caacgaaatg ttggatagca
     5101 tcaatcagtt tgtagaagac atctacaaat tagaaatcaa acagcaagac gcacatatgc
     5161 gggcgcttca agcacagatc aatccacact ttttatataa tacattagaa tatatccgaa
     5221 tgtatgcaat cagcgaagga agtgaagaat tagcagatgt tgtgtatgcg ttttccgctt
     5281 tgcttcgaaa caatacgaat caagagaaaa cgatcacttt aaaagaagaa ctggactttt
     5341 gcgaaaaata tgtctatttg tatcaaatgc gttatcctaa tcgagtagcc tatcatttta
     5401 tgatcgatcc tgatttggaa aagatcgaag tacctaaatt tgtgatccag ccattagttg
     5461 aaaattattt taaacacggt attgatttta ctcgattcga taatgcgcta agtgtcaaag
     5521 tcttgcagga aggcaaacgg gtaaggatca tcatcaaaga taatggaaaa gggatgactg
     5581 aaaaacggct aaaacagatt gaagaaaaac tgtctcatcc taaagtggaa cttcacggtt
     5641 ctatcggtct gcaaaacgtg aatgaacgtt tacgtgccag cttcggcagc agttattata
     5701 tgtcgcttga gaataatgag acaggtggac tgaccgtttc tattacattc aaggagggat
     5761 gagcgtgtat caagtgttat tggtagatga tgaatacatg attttgaatg ggttaaaaaa
     5821 gataattgat tggcaaagtt taggctttca aatagtagca acagcagaga atgccaaaga
     5881 aggactagct gtacttgaac agcgacagat tgatttagta gtaacggatg tgacaatgcc
     5941 agaaataaat ggactggaat ttattgaagc ggctcaaaaa gaacgtcata attttgaatt
     6001 tatgatttta tcaggatacc aagaattcga ttatttgaaa ggaggtatgc aattaggcgc
     6061 cgtgaattat ttgatgaaac ccgtgaataa gttcgaattg gttgaaagcc tcaagaaaat
     6121 caaaacacgg ttagatcagc aaaatgaaca aaaaaatcaa caagaaatct atcaagaact
     6181 gttattcagt caatggttga atgaagaatt ggatgaaata agtgaagaag aaataatagg
     6241 acaaataagt gagaagcagc ataggatagt attgattcaa ctggcaagaa ctcatgatac
     6301 tcttatcaat agatggttgc aagaacggca acaaatcttt tactatcaac gaaattacgg
     6361 agaccgtgta ttgatcacgt tgttgttgga ggcagatgaa gttgatggct tctgttgttt
     6421 tgttcaagaa aactttcctg atcaggaatg gctgatcagt atcggggaag agacagattc
     6481 tttggacaaa atccctgaaa cattccaaca agcgaaagac aatctacagt tgcatcaatt
     6541 ttatggagat aaagacgagc atgtttttta cgcagtacaa gcagaaacaa aagatcaaac
     6601 catcgatttt tcaagtttca gacgcgtgct gcaaaataaa caattggaag tcgctcaaaa
     6661 aatgatcact gatttttttg aacagtttca gttagcagcc atgatgccgg aagatatacg
     6721 atattccagt tttttactgt ttatggagat tcaacgggaa ttgattgatc tggaagatga
     6781 ggaatattta caaggaatcg aaaaaatcca tcaggcaaaa acggtgcaag aacttcagca
     6841 gctacttctg tcattcatca aaaagcatca gagacagaaa aagtacagtg acaatgtgga
     6901 aaaagtaatc gtaatcctgc accagcatta tcaagaacca cttacactca aagaagtgtc
     6961 agaaaacctg catttaaatg tcatgtatct aggacagctg ttcaaaaaag aaacgaaaaa
     7021 aagtttttca gcttatttga accatttacg tatggaaaaa gcgaaacaac tgcttttgca
     7081 cagtaatcaa aacatcaatg agatagcaag tgagattggc tacaacaaca ccacgtattt
     7141 ctctaaatta ttcaagaaaa tcgtcggcag atcccctaaa gagtaccgag aaaacagtgg
     7201 agaatcataa aagaaaaagc caatgtaatg aaataactta taaaaaattg tctagaaatc
     7261 taattttggg ctgttcttat tacttgaggc aaggcagctt tttcatgacc tctcgataaa
     7321 cgg
//
LOCUS       DACECZ010000120         6982 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.123, whole genome shotgun sequence.
ACCESSION   DACECZ010000120 DACECZ010000000
VERSION     DACECZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.123"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            99..746
                     /locus_tag="IWO35_002344"
     CDS             99..746
                     /locus_tag="IWO35_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0317382.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1139..3784
                     /locus_tag="IWO35_002345"
     CDS             1139..3784
                     /locus_tag="IWO35_002345"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ0317383.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="IWO35_002346"
     CDS             4094..5407
                     /locus_tag="IWO35_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ0317384.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="IWO35_002347"
     CDS             5397..6050
                     /locus_tag="IWO35_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0317385.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6776
                     /gene="radC"
                     /locus_tag="IWO35_002348"
     CDS             6105..6776
                     /gene="radC"
                     /locus_tag="IWO35_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAQ0317386.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
BASE COUNT         2282 a         1190 c         1486 g         2024 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccctatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttccggaata tataagttag
     6661 tacacataaa ttatataagg aaatttactg ttatattgcc atctggaaat actattatgt
     6721 tatcgataat tgaacgccat aatgcatttt tttcttcttc cgaagattct ttataaattt
     6781 catcgaattc ttttgataga ataaattcga cattttttat atctatttta gctggctcta
     6841 cttcaatttc atttaattct ttcattaaag cttctcgatc ttttttgtat tcctctaagt
     6901 ctataacttc attcaagtag gctttcttca atctatctaa tttattctca atatttttct
     6961 tactattttt ctttttaata at
//
LOCUS       DACECZ010000121         6968 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.124, whole genome shotgun sequence.
ACCESSION   DACECZ010000121 DACECZ010000000
VERSION     DACECZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.124"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            16..1170
                     /locus_tag="IWO35_002349"
     CDS             16..1170
                     /locus_tag="IWO35_002349"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ0317387.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1192..1944)
                     /locus_tag="IWO35_002350"
     CDS             complement(1192..1944)
                     /locus_tag="IWO35_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0317388.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2308..4974)
                     /locus_tag="IWO35_002351"
     CDS             complement(2308..4974)
                     /locus_tag="IWO35_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0317389.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5315..6766
                     /gene="cls"
                     /locus_tag="IWO35_002352"
     CDS             5315..6766
                     /gene="cls"
                     /locus_tag="IWO35_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0317390.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2049 a         1446 c         1272 g         2201 t
ORIGIN      
        1 taaagaggtg ttgaaatgaa aatagggatt gatcgtcttt ccttttttat tcctaattta
       61 tatttagata tgacagagtt ggcagaaagc cgtggggatg atcctgcaaa ataccatata
      121 gggattggtc aagaccaaat ggcagtcaat cgtgcaaatg aagacatcat tacactagga
      181 gcaaatgctg ccagcaaaat cgtaacagaa aaagaccgtg agctaatcga catggtcatc
      241 gtcggaacag aatccgggat tgatcattca aaagcgagtg cggtaattat ccaccattta
      301 ctgaaaatcc aatcttttgc tcgttctttt gaagtaaaag aggcttgcta cggtggcacc
      361 gcagctttgc acatggcgaa agaatatgtc aaaaatcatc cagaacgaaa agtactagtc
      421 atagcaagtg atattgctcg ttacggcttg gcaagcggtg gtgaagtgac gcaaggtgtc
      481 ggtgctgttg cgatgatgat cactcaaaac ccgcgtattt tatcgattga agacgacagc
      541 gtatttctga cagaagatat ctatgatttc tggcgtccag attatagcga atttcctgtt
      601 gttgatggtc ctttatctaa ttctacgtat atcgaatcat tccaaaaagt ttggaatcga
      661 cataaagaat tgtcgggtcg aggactcgaa gattatcagg cgattgcttt ccatattccg
      721 tatactaaga tgggaaaaaa ggcattgcaa agcgtattag accaaacaga cgaagaaaat
      781 caggaacgtc ttatggctcg ctatgaagaa agcatccgtt acagccgacg aatcggtaat
      841 ctttacactg gttcattata cctggggcta acttctctac tggaaaattc gaaatcacta
      901 cagccaggag atcgcatcgg tctattcagc tacggttctg gtgcagtaag tgaatttttt
      961 acaggttatc tggaagaaaa ctatcaagaa tatctcttcg cacaatctca tcaagagatg
     1021 ctagattctc gaacacggat cactgttgac gaatatgaaa cgattttcag tgagacgctt
     1081 cctgaacacg gagaatgtgc agaatatact tcagatgtac ctttttcgat tacaaagatc
     1141 gaaaatgata ttcgttacta caaaatataa taaaatcata aaaagaaccc ttcacgagtc
     1201 gtgaaaggtt ctttttttat ctgcggacca aaggagcagt ctataggtca gcttagtcgt
     1261 actaaggatt tctcgttttt cactgaatag tttgcgaatg tacctggtca caacaggatc
     1321 ttttgcgtac gcgtcatagt atagcttcat caattcttca gtaattgatt cctgtgtaaa
     1381 ataagtaaaa tcttttttct tcactttgaa tccttcatca attaagtatg tttcaaattg
     1441 atccattaca actggcgatg tattaggatc ttcttctatc attcctagtt gcttttcgat
     1501 aggataaaaa acatcatcga ttgattccac tactctgatt atcacgcacc atttgcttga
     1561 ctttttcatc aatttttcta attgaataag atgtaccaat gctggatttg ctgctgtaaa
     1621 aaccatttca tatgtctcat ttgattggat aaattcttct aacgactgtt tgataaagga
     1681 aacatttggc aactcatttt tttctgcttc tttttttgca ttttctagca ttcctgaaga
     1741 aaaatcgatc aaatcatagc gattaatttc ttttgctaag gaaagaaatc tgccagatcc
     1801 tccagcaacg tctgctgctg tttcagcagg caggactttc tccgttttca gataattttt
     1861 tacatcttca gcaatagtta cttgcgattc ttgctggatt tcataatatt cttcagcaaa
     1921 ttcatcccat tctttttcat tcatttgatt tcctctaatt ttttgttata tgttttcact
     1981 tttcggaata aatcattctc ctcttttatc tattctatct cttttgatga ataataaaat
     2041 gattggcttt ccgtctatca taaataaaaa aagggactgt gaccaaagtc gtgtcaaagt
     2101 ccctctttcc gaataaacag tgtccaatca gatgaccctc agtattagct caaaatgctg
     2161 attttacaac ttcttcctta aacggtgttc tcctgccaac tcttcggtca caagtcacaa
     2221 tattcaacaa acatgagaag ctgtttgttg aaattgcacc ttgtgactca gaattaaacg
     2281 gcaggctcac aacctcgttg attcgtttta tttatttcct tgtactcgta caatgatcaa
     2341 tttagtgatt tccatcaaaa caattgtcac aagtgcgatt cccatacatg taaagacttc
     2401 tgtcaatcca aatgctgatg ggatagagaa gacttctttc agtcctggga tcattgttaa
     2461 gcagtaaagc gcgctacata caactacagc tagtaagaca cttctgttag agaagaaccc
     2521 tgcttggata gctgtttgtg tgtttgaacg tgctgggaat gtttgcaatg tccgacacca
     2581 aatcaatgtt gaaaatgaca tggctacacc gaattcaggt gaatgctgca tgccaagcca
     2641 ttgagcaaag ataacagcaa tcccaatcag cacaccacga tacgttacag aaatcaatgt
     2701 tttgtccgta aagattcctg aatttggatc tcttggcggg cggttcatcg tagcaggttc
     2761 agctttttct gtacctaatg caattgctgg cagtgaatca ttgactaaat tgatgaacaa
     2821 cagttgtaat gctgtaaatg gattggacca gtcagcaact aaggcaaaga tgatcgcaat
     2881 gatcgcacct aaatttcctg caaatagata agaaatagct ttcttgatat tatcaaacac
     2941 attacgtcct acttctactg cgctgacaat agaagcaaag ttatcatccg tcaataccat
     3001 cgctgctgca tcttttgcca catctgtacc cgttcccata gcaattccga tgtctgcttg
     3061 tttcaatgct ggtgcgtcat ttacaccatc acctgtcata gctgagatat gacctttatt
     3121 ttgccaagca cgaacgatac gaattttatt ctcaggtgaa acacgtgcat aaacagtgac
     3181 tttttctaaa acttcgttta gttgatcttc tgtcaattca tctagttccg tcccggtcaa
     3241 agccaggttt ccttcttcaa aaatcccgat ttgttttgcg atagcaacag ctgttgtttt
     3301 gtggtcacca gtaatcatga ttgttttgat tcctgcactt cttgcttctg ttaccgcctg
     3361 agtcacttca gcacgtggag gatcgatcat agcaagcaaa ccaacaagga tcaaatcgtc
     3421 ttcatcttca aatgataaat caaaattatc aagtggacgg taagcaaaag ctagtacacg
     3481 taatgcgcgt gttgaaaaag cttcattttg ctcttggaat tttttcatca attcatccgt
     3541 catcggaacg acttcaccat ttagcaatac ttttttactg cgttggaaga cgatatctgg
     3601 tccgccttta acaaacaagt aatttttgtt atcaattgtg tgtccagtag acattaattt
     3661 tcgatctgag tcaaaaggaa gttctgcttg tcttgggtag ttgtttcgga tttcctgata
     3721 aggctggttt aatttttcac taaagtcgat aaacgctact tccgtagggt cgcccaattt
     3781 agtaccgtct tcactgatcg atgcatcatt cgctaaaaca ctgatttcga tcaagcgacg
     3841 ttcgtcagct gtccattttg aagggtcatt tgtgaaatcc ccagtatcgc cattacctaa
     3901 aaagtagtca acaactgtca ttttgttttg tgtcaatgta ccagtcttat ctgtacagat
     3961 gatacttgtc gaaccaagtg tttcaactgc tggaagcttc cgaataatgg catgacgttt
     4021 tgccatttta ttcgttccca aagataacac gattgttacg atagactgta atgcttctgg
     4081 aatagcagct acggctacgg ctacggcaaa catcactgcg ctgacgatat ctgcagtcat
     4141 atctcctgta ccttctccaa gataaatacg aaacagttgg atacctaaaa tcaataaaga
     4201 caatgccaaa atagccaaac ttagtttttt actgaattta tccagacctc gttgtaacgg
     4261 agtgacttgc tcagttgttg attccaaaag gccagcaact tgtccgatct ccgtattatt
     4321 cccagttgaa gtcactaaga atttaccgcg tccgtaagtt acgatcgttc cactgaatac
     4381 catatttgta cgatctccga ttggtaccgt acttgtgata tccgttactt ctttttctgc
     4441 agaagtcgac tctcctgtca gcataccttc atctactttt aatgatcccg catctagtaa
     4501 acgtccatca gcaggtacat aatcgcctgc gtctaacaac acgatatctc ctaccacaag
     4561 ttcgtttgcc ggaatcgttt tttccttacc atcacgaatc actcgagcat cgggcgctga
     4621 aaggtttttc aacgcttcta aagaaccttc tgcttttttt gtttggacaa cactgattat
     4681 agaattcaac atcaatactg caaaaataac taagaattcg atgtagtcac ccatgaccat
     4741 ctgaatcagt gcgacaatca gcaatacgat aaccattgca tctttgaaag tttctaagaa
     4801 caatttccaa gttggcttct tcggcttttc ttttagctgg ttcaagccat ctctttctaa
     4861 tttttccttc gcttgcgtgg tgctcaatcc atcggttgag ctctgcgttt gctgcatcaa
     4921 cgagtcgatc gtttctttat agaccgcttg ttgtgcttgt tccttgtttt ccaaatttca
     4981 tcccccattt tttgatcttg gccctagtcc tgtctgaaaa aaaagagacc taagacaaat
     5041 acgcatttgt cttaggtctc accatttcat acaataccag aaaaccattt ggttaactta
     5101 ctgttgatat tatcgcaaca aacgttgccg gttactcccc taaggagata ttcaactgta
     5161 ctaatatcct accttattaa cctacaaaaa gcaactactt tagcccgatt tacctacatt
     5221 ttcatgaaag gagtgaaaag tatattcacc ttggtaggct tctttcttct atcatgctat
     5281 actaatgtta ttgtatgtaa ggaggaacat ttttgtggta tctagtatta taaccgccct
     5341 ttatctatta aatgcactta ttgctttggt ggctattttg atcaaacccc gagatgtagc
     5401 agccatttgg gcatggctct tagtattgtt tgcccttcct ggggtgggct ttgtattata
     5461 tttatttttc ggacgtggat taacggataa gaaaaaattc tatctccgac aaagtgactt
     5521 gaaagaatta gaaaactttc agtcttttag aggagatacc attgaacatt acgatcctga
     5581 catgggcgat aaagacaaac agcaatttgt tgacttcttc tcttcattaa atcgtatgcc
     5641 gctgacaaga atgaattctg tcactcttct cacagacgga caagagaaat tggattcact
     5701 gcttcaagat ctaaaaaaag ccaaacattc gatccatatc gaatattacg catttgtgac
     5761 agataatatc ggccagcaag tcttacatgt tttagaagaa aaagccgcag aaggcgtgga
     5821 agttcgaata ttatatgatg catttggctc tcatggcaca aaagcaaaag atttcaatcg
     5881 tctaatcaaa aatggtggac atgtccatac atttgttacc tcacaaaggg cattacttcg
     5941 tttccgattg aattaccatg atcaccgaaa aatcgttgtg atcgatggaa agattagtta
     6001 taccggtggt ttcaatattg ccaatcaata tgtaaataca acaaaaaaat tcggctattg
     6061 gcgcgatacg catatacgga ttttcggtgc cgcttcttct ttgctccagc ttcgcttctt
     6121 aacagactgg aacgtctcgg tacctgaaga aaaaaaggtc ggctatcatt tgaattattt
     6181 ctttaaaaaa gcagatcgag atgaatctaa gcttgctgat acatccatcc agcttgtttc
     6241 aagcggaccg aataacgaaa gggaacaaat caagctttca tttatcaaat tgattacttc
     6301 tgctaaaaaa cgtgtttgga tacagacacc ttaccttgtt cctgatgaaa gtgtcattgc
     6361 tgctttaaaa atcgcaactg cctctggtgt agatgtgaaa atcatgattc ccaacaaacc
     6421 ggatcatcct tttatttatc gagcaacaca atattatgct cggcagctga tcaaggaaaa
     6481 tgtacaaatc cttgtctatg agaacggctt cctccatgca aaaacattga taatggatga
     6541 tgaaatctgc atggtaggtt cagcaaatca agatattcgt agctaccgat tgaattttga
     6601 aacaagtgct gtcatttacg atcctgagtt tttagaagaa cttgctactc agttcaaaga
     6661 agatgagaca cattgttcat ccatgacaac tgaaacagtc aaggaaatgt ctaactggct
     6721 attattcaag caacaaattt ctcgattatt ttctccaatc ctataatttt tttattcaag
     6781 tagagaaaaa tatactaaga ttaaagggac tgtgacaaaa atcttgtcac agtcccttct
     6841 tccgaacaaa cggtgttctc ctgccaactc ctcggtcaca agtcacaata ttcaacaaac
     6901 atgagaagct gttttttgaa cttgttcctt gtgactcgga gctggacggc aggctcacga
     6961 cctctttc
//
LOCUS       DACECZ010000122         6776 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.125, whole genome shotgun sequence.
ACCESSION   DACECZ010000122 DACECZ010000000
VERSION     DACECZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.125"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            9..899
                     /locus_tag="IWO35_002353"
     CDS             9..899
                     /locus_tag="IWO35_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ0317391.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(972..1796)
                     /gene="nadE"
                     /locus_tag="IWO35_002354"
     CDS             complement(972..1796)
                     /gene="nadE"
                     /locus_tag="IWO35_002354"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ0317392.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1808..3280)
                     /locus_tag="IWO35_002355"
     CDS             complement(1808..3280)
                     /locus_tag="IWO35_002355"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ0317393.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3917..4279)
                     /locus_tag="IWO35_002356"
     CDS             complement(3917..4279)
                     /locus_tag="IWO35_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0317394.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4502..5254
                     /locus_tag="IWO35_002357"
     CDS             4502..5254
                     /locus_tag="IWO35_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0317395.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5394..6317)
                     /locus_tag="IWO35_002358"
     CDS             complement(5394..6317)
                     /locus_tag="IWO35_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0317396.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6332..6604)
                     /locus_tag="IWO35_002359"
     CDS             complement(6332..6604)
                     /locus_tag="IWO35_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317397.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2068 a         1350 c         1158 g         2200 t
ORIGIN      
        1 tgatatatat gcaactacaa ataaacgaag aatccttacc ggtatatgaa gcattagcta
       61 gtaaaacaag gatcaagatc atccagctac tttccaaaaa aaagatgaat gtcaaagatt
      121 tggcaaaaga acttggagtg agcagtgcta tcacgacgat gcacgtgaaa aagctagagg
      181 aagcaaacat catcaaaaca gaaaaagtag gacaacaaaa aatttcgagc cttcgtgtcg
      241 ataaaatcga tatcagtttt ccagaaaaaa tattcaatgc ttttgatacg aaagaaacat
      301 cgatacctat tggtcactat acaaattatg caattgaacc aacatgcggt ttagctacga
      361 tccatgattt tatcgggaaa gtggatgaac cacgctattt tatggatcct cgaaggatgg
      421 atgcccgcat tctctggttt accagcggat ttgtcgagta tcaggcacct aattttttaa
      481 atacagagga tacattggag atgttggaag tttctgtgga aatttcttct gaatttcctt
      541 tttcaaatga caattggcct tcagatatca ctttcagcct aaatggtgtg gagctaggaa
      601 catggacgag ccctggagat tttgctgata tccgcggtaa atatacgcct gattggtatc
      661 cagataatct taatcaatac ggcttgctga agacgatccg aattaccaaa cacttgacga
      721 atatgaatgg agaacccttg tcaaacatca cgatcaacga catccctaaa gagcaagaca
      781 cttggcattt gcgaatcgaa gtgaaagacg atgccaaaca tgtggggggc tgtactttat
      841 ttggtaaagg attcggtaat tacgatcagg atatcaaatt aaaagtttac tacagctgat
      901 atttttcttt tctattttat aaaacaaaaa cagccaatcc ctatgaaggg acggctgttt
      961 ttgctttatt gttatttcca gaaatcatcc aaaacggaaa ttggcaaatg tcgtttatgt
     1021 tgtgtcttgt tccaccatgc ttcgattttt tcttgtgctt gagcgggaac ttcttttcct
     1081 tctagataat catcaatttc attatatgtg acacctagtg cgacttcatc tgcaatcatt
     1141 ggtttcccat cttcaagatc tgcagtcgga atttttgtgt ataatttttc cggtgcattc
     1201 aatgcttgaa gcaattgttt cccttgtcgt ttgtccaaac ggaataatgg caaaatgtcg
     1261 gctccaccat caccaaattt cgtgaagaat ccagtaatgt tttctgcagc atgatctgtc
     1321 cctaatacgg ctcctgctct ttctccagct acggcatatt gcgtaatcat acgttgacgt
     1381 gctttgatat ttcctttgtt aaaatcgctg attcccacac ctgcttcgct taatacttgt
     1441 acttgtgcat caacagctgg tttgatattt actcgcagac taacatcggg ctggataaag
     1501 tcaagagcag cttttgcatc ttcttcatct gcttgttcac cataaggaag acgaacagcg
     1561 ataaattgat agttttcatc ttttgtttct tcacgcattt cttccatcgt tagttgcgcc
     1621 aaacggcctg ctaaagtaga atcttggccg ccgctgatac ctagtacaaa agttttaaga
     1681 aacggatatt ttttcaaata tgctttcata aagtcaatac ttttacgtat ttcttctttt
     1741 ggatcaattg ttggtttgac acctaattca ttgataattt ctgtctgcag gctcatttat
     1801 tctcctccta tgcttctgta tgaagttctg ctacgctttt tttcattcgt tccatgatag
     1861 ccattttatg gttccagcat tcagtagaca gatcgactgg gtatttttgc ggattcaagt
     1921 ctcgtttata ctcttcccat agtgaatcta gattttcttt tgtatattct ttgatttcgt
     1981 ttagtgcagg taattcgtaa actctttttc cttcgataaa gatatcttgt aaaactgggc
     2041 gagctgtaaa gtctcgaaca gttttattga taaacgtatg cactggatgg aacatgaaga
     2101 tagcttcttc ttctcgaggg tcttcatccc ataatgtcac gtagtctcct tcagatttcc
     2161 catcaaaatt tcgagtgatg cgccacactt gttttttccc tggagtagtt actttttctg
     2221 cattgcttga taacttgatc gtatccatca tttttccttc atcatcttcg atcgatacta
     2281 gcttgaatac tgcaccaagg gctggctgat cataggctgt gatcaatttt gtcccaacac
     2341 cccatacatc aattttagct ttttgcatct ttaaattcaa aatcgttgct tcatccaagt
     2401 cattcgaagc atagattttt gcttccgtaa atccggctgc atctaattgt tcccgaacac
     2461 gcttagatat ataggccata tctccactgt cgatgcgtac gccttggaag ttgattttgt
     2521 cacctaattc tttcgccacg cgaatcgcac ttggcacacc tgatttcaac gtatcataag
     2581 tgtcgactaa gaacacgcag tctttatggg ttttagcgta ggccataaat gcttcataat
     2641 catttccata agattggact aacgaatgag catgggtccc gctagctggt atcccaaata
     2701 tcttcccagc acgtacattg cttgttgcat ctgctccgcc gatatatgcc gctctagttc
     2761 cccagactgc tgcatccagt tcttgtgccc gtctagttcc aaattccaat agtggatctt
     2821 ctccgatgac agatttgata cgagctgctt ttgtcgcgat caacgtttgg aaattgacca
     2881 tattcaacag tgctgtttct actaattggc agtgcgctaa aggcccttcc acttggatta
     2941 atggttcatt tgcaaaaact agttctcctt cacgagcaga acgaactgtt gctttaaatt
     3001 caaaattttg taaatactcc aaaaaacctt caggatagac ttctaatcca cgcaagtaag
     3061 caatatcgga atcattgaac gtcagattct ctaaataatt gactaaccgt tccaatcctg
     3121 caaaaaccgc gtatccgtgg ttaaaaggca tttctcgaaa gtaacattca aaaacagcat
     3181 gtaaatcagc tcgacctaat tcccagtatg tctgcatcat attgatttga tatagatccg
     3241 tatgcagcgt caaactgtcg tctgggtaag tattattcat cttgtattac tcctcttggc
     3301 aagttttcat ttcctaccca ttataacaaa aaaaattaat aggaaaagaa atcttccatt
     3361 ttttctgaaa aagcacatgt tttacggata tttcttgcta aaaatgaact ccatctgcta
     3421 aaactatcct ttcttattgg tatttaacct aaacgagaaa gaatacttat tttattcaat
     3481 gtatccgaga cacgaagaaa ttagcgtttt ctatttttct tacttgataa aataagtaga
     3541 ttttcctact acaatcgtag ccgttaagat accgatgatt gaacaaatat aaccaaatgt
     3601 ctctacaaca taatcaatgg caaaagtttt tgaagcaata atacgtcttt ccttttttgc
     3661 ttcttatccc caaagaaacg gatataactt attatgtatt gaattttttt ggaaaggcga
     3721 attatgacaa gatatacctt gcgttgaaaa aactcgcgga aaaaatatca cacgttttta
     3781 aaaagataca aagtaagaaa gcagctgtca atagaatgga aacaaaaatg gcgagacatc
     3841 aatcagtctc gccttgtttt ttattgtata cattagatat ggagcaggtt accataaaga
     3901 tgattattag tcgttattaa atagaatcct tcgtattttt ttgttctatc actacaatat
     3961 ctttatttgg acgtttgaat tgtattccgg cacgttctag ctcctgtttc actcctacat
     4021 ggactttgac atcttctcgt gtcattttta aaacgtgatg aatcgcatag tcgatttctt
     4081 gccttgtgat ttttttcttc gtattgatca tcacgacttg ctttttatca tatgtgtcta
     4141 ccaatagcag ttgtgttcga tctaacgaaa ataatttgta ataattaata gccgtattcc
     4201 caaaaaagaa ttccactact ttaggataaa atgtcttaat atttaaatta ctattcacag
     4261 gtgtttcgat tagcttcatc tcattcctct tttctatata gtcgatttag atccaatctt
     4321 aagttatgtt accatctttc ctagcattaa ggaatcggtt acaattgaac ctttaaagac
     4381 aaaaggattt accttttatc acttaatagt ataacataat ttcgtgagaa aaaaatacaa
     4441 ttttttcttg accatctcgt tacgtaaagg attatgctta catttgtcag gaggaaaaat
     4501 aatggagtac acaataaaga aaatgtcaga gatatcagga gtcagtccga gaaccttgcg
     4561 cttttatgat gagattggac tgctaaaacc agctaggatc aattcttcag gttatcggat
     4621 ttatggcaaa aaagaagtcg atcgtctgca acacatctta ttttatcgaa caatggcatt
     4681 caagttggat gatatccaag aggtcttaga caatccatct tttgaccacc agaaagcttt
     4741 aatcaagcat cagcaaatgt tgctggaaaa acgggcacaa atcgataccc tcttgacaac
     4801 tgtccaacag acacttgata tgtacgaagg aggaagaaaa atgagtgaca aagaaaaatt
     4861 tgaagggttt aaacaacaaa agttgaaaga aaatgaagaa tcctatggac aagagattcg
     4921 acaaaaatac ggaaaaaaaa aggttacagc atccaatgaa aaatggatga atatggataa
     4981 agaaacgtat gaagaaatgc aaactgttga aaagcagatg ttatttgatc tcactcttta
     5041 cttaaaagaa ccagaagatg aaagcttagc agaccgtatc ttccaagctc ataagaagtg
     5101 gttctcttac agctggcctg aatatcagcc aaaagcgcat aaaggactag gaatgatgta
     5161 cgaagccgat gaacggttca ctgcatatta tgacgagcga agtggtcatg gtgcagcaaa
     5221 agctttaaat gaaattatcc aaaaaaaggc gtaatctttt acatgatcca acaaaaaagt
     5281 acacgtaaaa tgacctaata acaaaacagc agacacccaa gttagatttt tatgctaact
     5341 tttggtgtct gctccattct ttcacaagtt ttttcatcct actttaaata tattcatcgt
     5401 tgaataaacg tataaggtat tttcacttta tccttttttt ctgtaagaaa caattgaaat
     5461 gccatttctc cgcaagcttc cagatggtgg tcgatggttg ggaaaccaag aagctcgctg
     5521 atcagcaaat tatcgcgtcc aatcaccact ggtgtcttat cttgcagata gctttgcaag
     5581 atgcctgctg caaccatatc cgaatttgta aagacaccat caactccaag ctctttaaaa
     5641 aagaagcctg cttgttttcc tttctctgca tctatacaat cccaaaaaac aagtgattca
     5701 tcaaaagatg gaaaaagttg atgacatagc tgcaaagtga tttttgagtt ataacttagc
     5761 ctgccacttc gccccaacgt gatgcccaat tgctttatac cttgctgctt caaataagta
     5821 agaccttctg taattgattg ttctcgatca ataaagacac agcttgcctg ttttcccttg
     5881 atttcttcac aaaaaactag aggtccatat ttttgatatg ccaataaatc atctagtgta
     5941 ttggcacgag acgtaatgat caatccatcg aaagctttag ctgcaaattc ttcaagatac
     6001 cttcgctctt tttctttatg atagcccgtc ggtaaaagtg tcactttgta atcataatca
     6061 aacgctgctt caatgatccc actgatcaac tgatcaaaat acggatgatt ggtataaggc
     6121 agaatcactc caatgctttt cgtttttcca taacttaact gtctggcatt tgcattaggg
     6181 atataatcga cttctttcat gaccgcaagt atttctttcc ttttttcctc atctacatat
     6241 ggatgtccat tgattacacg tgaaatagta gaaactgaat aacctgtgag ctttgcgata
     6301 tctcgaatgt ttgccactgc tgcttcgcca cctatacaaa aagattttct tgtaaatagt
     6361 ctaccacagc tggaccgcat tcaaaaaact gatacatttt tccttcatgc gaaaaactga
     6421 tccgctgccc gccccaatat atatcttttg tcaaattttc gatttcattc attggtattt
     6481 cttttactat attcctattc aacccttgaa aactgtacaa cgttaatacg gatcccttga
     6541 actcgacaaa cgcataaaaa taattgttcg ttgtgccatc tgttatctct acataagtat
     6601 tcattgcaag ccctcctttt gatactttga gtatagtgta tgaaacgcgt ctcatagcaa
     6661 ggagtttgtt ttgccaaaga aaagttttat tcctctttca ttattaccaa agtctcttat
     6721 tttaaattga atacattata atgatactgt aaatatatct ttattattag tgaatg
//
LOCUS       DACECZ010000123         6693 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.126, whole genome shotgun sequence.
ACCESSION   DACECZ010000123 DACECZ010000000
VERSION     DACECZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.126"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(718..3867)
                     /locus_tag="IWO35_002360"
     CDS             complement(718..3867)
                     /locus_tag="IWO35_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ0317398.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3880..5079)
                     /locus_tag="IWO35_002361"
     CDS             complement(3880..5079)
                     /locus_tag="IWO35_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ0317399.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(5069..6664)
                     /locus_tag="IWO35_002362"
     CDS             complement(5069..6664)
                     /locus_tag="IWO35_002362"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ0317400.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
BASE COUNT         1866 a         1380 c         1093 g         2354 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttgggca cttgaatctc ggctattttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcgtccttc acgaatgagc tgattgggaa actcttttgt
     2041 taaccaatca ggctctttcg tcatctcttt gatggcccga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga ttgcagattt cgttggcctg ccatttcgaa aatcaaattt
     2161 tgtataggca ttatctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gctgggtcca aataactttc ttttttgaca ggttccattt gatcaatcat
     2281 acgattgatc tgttctgatg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctagtgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 gacagagcca tcttctagcg cattcttaat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtt gtacgtgcca attgcccttt ggcttgtttc cgattctcag gaaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt ggcccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttc tttgcatatt tcaacgccgc
     2701 atctagtttt tgacgggttg taatgattac aacatttgca ttggcatctg ccaacagggt
     2761 ttcacttaat tctttggcac ttcccgttcc aacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaattttgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgttcgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ctttcctgaa ccagtcgcat gccacacata
     3001 ccctgattga tgtttatttg cggcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgagggttt tattatcttg atcttcactg acgattgtat aatccgcaat
     3121 caaacgatga gcatccggga tatttaacac ttgtttacaa aactcatata agttttctac
     3181 tttttcattg tctttcgttc gccaactaaa taaaaactta gggtgcaagt ctttcggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaatgcctg
     3361 ataaacacca tcttttgcag ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gattaataag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aagtagagat tcctccacca atgtcttgat tggaataaag
     3541 gataagtgag acagaaccca attgcgaatc ttcccgttca atcgtgatcc gagccatccc
     3601 attttcacct tttaaccatt tcgcagcatc aaatggggtt ttcgttcgca gcagtaattc
     3661 cgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagata attttttggc gtaaattgac ccaaaggtct tcttctgatt ttagatccgg
     3781 acgatacgtc cactgattat gcccttctcc aagtacacga atcaactgtt cttcgacttc
     3841 tgcttcctca cggtgactaa ttttcattct tcttccctcc tagacaaaca tcttttgtaa
     3901 gaagcttttt ttcgtttctt ttagtaaatc taacttacgt tgatgaagag cgatcgtatc
     3961 atctagctgt ttgaagaaag taccgatttt ttgttgttca tcctttacag gaaccttaac
     4021 actgatattc tcaatatcag aactgttata agaaggcaat gcccctactt gggagtatct
     4081 tggcaaattt atagtttcaa acaatatttt accaaaatag atatcccaag tattattaaa
     4141 taaataagcc atggtattgt tatctattaa gaaagggctt gtgaccaaac gttttctatt
     4201 aagcattatt gctgcgccaa ctttagcaaa tataatcgct ggcacatctt ttattggctt
     4261 ccatttttta cgctcaattt gctcattaga tacataatga tttgcatttc tcatttcata
     4321 ctcattacca atgttattca tgtcggaaac tttaaaaaat ggagtacctt cagaaccacc
     4381 ttgctcggca tcaggaaatc caattcccga ttgagtttta cctaattctc ccaacttacg
     4441 ctcttcccaa tcttccgtaa aaccaggaaa ccggatttct ggcacttttg ctccgttttc
     4501 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta acttacgttg
     4561 atgaagagcg atagtgtcgt cgagtttttt gaagaatatg ccgatttttt gttgttcttt
     4621 taaatcattg ggaatataga atactgtatc tgaaactttt ttccagtctg aacgaggcat
     4681 ttttgttcca gatgtatcat ttgcagcctt ttgatatcta tcagcttgaa taagagaata
     4741 aataaagtcc gagtcgctct tcttagctct aaaaacccag aaatccccaa tcgctacgcc
     4801 tgtaaaagtt gccttgagcc aattttttaa atatggacgt aactttccat atagaatatc
     4861 atttggtaaa aattcaatcc cttttctatt atcaaatttc tggctcacat ctttattcag
     4921 tctaccttct cctgccacga tatcttcaaa ttcaactttt ggaagtctac tggagtttgt
     4981 acttttatta agtctaacaa ctaaatcaat taacttacgc tcttcccaat cttccgtaaa
     5041 tcctgggaaa cggatttctg gtcgtgtatc attgctcatg tttgaacacc tccagcgcac
     5101 cttgtaacca catttcgttt tcaggtgttg tttgcaggga agagatagct tctaaaagtt
     5161 ctttttctag cacttgctgt tcttgtcgta tcttcttgat gtcatttccg acatgaacca
     5221 tatcaattgg ttcttcttct tcgaaagtat ctacatagcg tgggatattt aagttatagt
     5281 cattttcttt gatttcatca aaggttgcca aatgagcgta tttctcgaca tcttttcttt
     5341 cagcgtaggt ttcgaggatt ttttgaatgt tttcttccga tagtttattt tgatttttcc
     5401 ctttaacaaa ttcacgacta gcatcaatga ataatacatc tcgagtttga cgattcttct
     5461 tcaacacaat caccgtcgtt ggaatcgatg tcccaaagaa caagttcgca ggcataccga
     5521 tcacagcata aatactgcca tcttctagta atttttgacg gatgacccct tctgcagcgc
     5581 cacggaataa aactccgtgt ggtaagacga tggccatggt acctgtttcc ttcaaatgat
     5641 agaacccatg aagaagaaac gcaaaatctg ctttggattt tggcgctaat ttcccataac
     5701 gattaaaccg agaatcatct aagaaagtcg tatctgctga ccaattagcc gaataaggtg
     5761 ggttcattac tactgcatca aatgtgtaag gttcatctgt tggccagtcc ttattcaacg
     5821 tatccccatt gcggagattc atttcttcgg cgtcgacacc atgtaaaatc aagttcattt
     5881 ttgctagatt gtatgtcgtt gtatttagtt cttgcccatg atatttaacg ttatctgggt
     5941 gtgttaagta gttacgtacg tttaacatca atgaacctga acccatcgtt ggatcaaaaa
     6001 cactaaagaa tcgtcgttcc ttttgatcga gcgttacgat ttgcgccatc atatcagaaa
     6061 ccatatgagg tgtgtaaaat tctccggctt tcttccctgc ttctgaagca aattgactga
     6121 tcagaaactc atacgcatcc ccgatcacgt ccccatcgtg ttctaaaaca tctacatcat
     6181 tcaatttctt gatgacttcg gtaatcgtca catttcgttg ttgctcatct gttcctagtt
     6241 tttttgattg gagatccaca tcatcaaata acccattaaa ttgattgtag gtagatgcca
     6301 attgcacaaa ggctttgttt agatcgttca attggaacgt tgcttgtttt gcttgatccg
     6361 ctaagacgtt gaaaagatac tctggtgcaa ttgcatatcc caaaatatca acgattgtgg
     6421 caatcagatc ttcttttgat tcttcatctg aaagtaattc acgatacaac attgtttgtt
     6481 tgcttactgt gtcatattcc tctaaggatt catctgctaa aagaaccact tgttccaaca
     6541 atcgatcgga taaatattta tagaaaatca gtcccaataa ataattttta tattctgacg
     6601 catccatttt actacggaga ttatctgccg cactaaataa tttttgattt aattctgcac
     6661 ccaaaaagat acactcctat ctcttattta aag
//
LOCUS       DACECZ010000124         6446 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.127, whole genome shotgun sequence.
ACCESSION   DACECZ010000124 DACECZ010000000
VERSION     DACECZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.127"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(105..560)
                     /locus_tag="IWO35_002363"
     CDS             complement(105..560)
                     /locus_tag="IWO35_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317401.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(714..1922)
                     /locus_tag="IWO35_002364"
     CDS             complement(714..1922)
                     /locus_tag="IWO35_002364"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ0317402.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2034..2366)
                     /locus_tag="IWO35_002365"
     CDS             complement(2034..2366)
                     /locus_tag="IWO35_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0317403.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2514..3389
                     /locus_tag="IWO35_002366"
     CDS             2514..3389
                     /locus_tag="IWO35_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0317404.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3471..4052)
                     /locus_tag="IWO35_002367"
     CDS             complement(3471..4052)
                     /locus_tag="IWO35_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ0317405.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4058..5317)
                     /locus_tag="IWO35_002368"
     CDS             complement(4058..5317)
                     /locus_tag="IWO35_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0317406.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5319..6365)
                     /locus_tag="IWO35_002369"
     CDS             complement(5319..6365)
                     /locus_tag="IWO35_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317407.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1791 a         1336 c         1100 g         2219 t
ORIGIN      
        1 cttgtcacag tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat
       61 aagacacatc tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc
      121 ccgtatacat cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt
      181 tcgctgattt ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat
      241 gggaagtaca caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct
      301 tcgtatttca atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt
      361 gtcgcaactg cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt
      421 tcaacaaatt cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga
      481 tcgccgatat gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc
      541 gctgtcattt ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac
      601 ctcagtttag cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt
      661 atctgcccac tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc
      721 aatttttctt tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct
      781 acgacggctt tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca
      841 gtttcttcta gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa
      901 cgatcatagg attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat
      961 tcttctactt gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct
     1021 ccagtttctc gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta
     1081 gaacacttcc acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg
     1141 atcaataatg cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa
     1201 cgtaaaagtg cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca
     1261 ataaatgcat ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca
     1321 tttatacgtt ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag
     1381 gtttcttcct ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt
     1441 aaggcatctt catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct
     1501 agatcttgca gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca
     1561 ttgatcgtaa aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga
     1621 tctggtctac ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt
     1681 tcatcatttt ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga
     1741 aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc
     1801 tggttcagca agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc
     1861 tctaattttt tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc
     1921 atttttccaa cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga
     1981 ctgtttgaca agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg
     2041 tccttacgct caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa
     2101 acttcagtta agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca
     2161 ccaagttctt ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa
     2221 gtgtggttga cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct
     2281 aatggagaga aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata
     2341 ctgtataaag aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt
     2401 aacgcaaatc ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag
     2461 gaatttctaa atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca
     2521 aacgaaaaac agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg
     2581 gtcttgttta cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct
     2641 tgatcttaag agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc
     2701 cattgatttt actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag
     2761 gtacactttc tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt
     2821 tacagcatga tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg
     2881 ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag
     2941 aacaaaatta cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac
     3001 ttgcttcatt aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg
     3061 ttttcagtcg tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg
     3121 tcgtctccaa caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg
     3181 tgactttcct ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg
     3241 tcgttagctc tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag
     3301 ctttcctttc aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc
     3361 cacagacaag attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag
     3421 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
     3481 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
     3541 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
     3601 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
     3661 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
     3721 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
     3781 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
     3841 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
     3901 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
     3961 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
     4021 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
     4081 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
     4141 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
     4201 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
     4261 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
     4321 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
     4381 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     4441 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     4501 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     4561 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     4621 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     4681 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     4741 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     4801 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     4861 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     4921 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     4981 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     5041 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     5101 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     5161 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     5221 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     5281 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     5341 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
     5401 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     5461 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     5521 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     5581 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     5641 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     5701 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     5761 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     5821 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     5881 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     5941 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     6001 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     6061 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     6121 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     6181 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     6241 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     6301 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     6361 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     6421 agataagagc aaagctcacc tcataa
//
LOCUS       DACECZ010000125         6423 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.128, whole genome shotgun sequence.
ACCESSION   DACECZ010000125 DACECZ010000000
VERSION     DACECZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.128"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(55..789)
                     /locus_tag="IWO35_002370"
     CDS             complement(55..789)
                     /locus_tag="IWO35_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0317408.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="IWO35_002371"
     CDS             1116..1526
                     /locus_tag="IWO35_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ0317409.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="IWO35_002372"
     CDS             1519..2220
                     /locus_tag="IWO35_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ0317410.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="IWO35_002373"
     CDS             2220..3806
                     /locus_tag="IWO35_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0317411.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="IWO35_002374"
     CDS             3825..4046
                     /locus_tag="IWO35_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ0317412.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="IWO35_002375"
     CDS             4043..4804
                     /locus_tag="IWO35_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ0317413.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="IWO35_002376"
     CDS             5184..5693
                     /locus_tag="IWO35_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0317414.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="IWO35_002377"
     CDS             complement(5768..6307)
                     /locus_tag="IWO35_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ0317415.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
BASE COUNT         2009 a         1177 c         1302 g         1935 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggt
//
LOCUS       DACECZ010000126         6273 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.129, whole genome shotgun sequence.
ACCESSION   DACECZ010000126 DACECZ010000000
VERSION     DACECZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.129"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IWO35_002378"
     CDS             complement(99..1229)
                     /locus_tag="IWO35_002378"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ0317416.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IWO35_002379"
     CDS             complement(1878..3080)
                     /locus_tag="IWO35_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0317417.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IWO35_002380"
     CDS             complement(3140..3343)
                     /locus_tag="IWO35_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0317418.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IWO35_002381"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IWO35_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IWO35_002382"
     CDS             4880..5692
                     /locus_tag="IWO35_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0317419.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1862 a         1287 c          996 g         2128 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tac
//
LOCUS       DACECZ010000127         6220 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.130, whole genome shotgun sequence.
ACCESSION   DACECZ010000127 DACECZ010000000
VERSION     DACECZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.130"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..467
                     /locus_tag="IWO35_002383"
     CDS             <1..467
                     /locus_tag="IWO35_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317420.1"
                     /translation="CDFLVYGETSELLKLYGIQRDNRASDFGKIIYTEEKEFNKKIFT
                     LLEGKQGVSPYDVFPDFDALYKKYMFAEYYIVYNYFLENIASNESYGDLMDAYYSYLK
                     KYAVVADSEKLLLYWPKYVQYNVKFENELDFYAKLDSKKWMDIYANYTPFSQ"
     gene            493..1578
                     /locus_tag="IWO35_002384"
     CDS             493..1578
                     /locus_tag="IWO35_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HAQ0317421.1"
                     /translation="MKIGIVTIVNGASFGNRLQNYATQKVLENNGYTVETINNCFDKE
                     STLSIVIKNAIYTLFKFIPHFKKSKSIWKRMYTFYQWDKQYIDYSHRKIDASKKNSFV
                     SIKNDYDYFLVGSDQIWNPKYAANKGFELLSFVPNEKRVSLSTSLGVPSIPSDLEKLY
                     VTEWNKFKSISVRENQAAKIIEDLTGTTAAVHIDPTLMLERSEWEAFQKKPDNIDENY
                     ILSFFLTNPPKDTVDVLDKVAKDLKIGVLTLDASWEYFQDITPNHFVYLISHARMIYT
                     DSFHCTVFSLLFGKPVVVFDRFDSEKMNSRLSTLLNKVNKAHFWVGKKADEAMIRAIA
                     SEPEYDIDKLMVSERECVKAYLKEYLQ"
     gene            1601..2416
                     /locus_tag="IWO35_002385"
     CDS             1601..2416
                     /locus_tag="IWO35_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317422.1"
                     /translation="MEHLSDCFGKMKIVTTSCAREYFKDDLDKSEYSCFVDSRAAMFF
                     AMGQALAKRRNVVVMLADELTSCYTALTECAFQQVDLIVFAFQTVRHEIDLASLKNCG
                     CNLINCSAQDDIDRVVSNTKGLNQTTVIKINMNCESDKDHICEYIDLPDIPVFFHGSL
                     YSPASKKKYEQYGQVSKYLGYLVGGKDAILIISHNEFLNDISVWINYYSFGEIQPKII
                     IFDSPNDYQYKWLCDLECSVAHSTNRFLSEDLALLQNNGISIVLRGDNYDIVS"
     gene            2400..4118
                     /locus_tag="IWO35_002386"
     CDS             2400..4118
                     /locus_tag="IWO35_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HAQ0317423.1"
                     /translation="MISSVKNVQILVSLLKQHNIKDIVISPGSRDFQLVHSVETDSFF
                     RTYTVVDERSAAFFANGLSMAIDKPVCVCCTSSTASSNYMAAAKDAFANGIQLVLLTA
                     DRDYRRLYQMEDQMIDQVDMYGAYVRSNVDIPIVKDSDDEWYATRKINEAILELNHGV
                     KGPVQINYQLMDLSAPFVETMPEYRKITRYQEDDLLSSVEIFKQKLLSKKRIMVLCGQ
                     QEENNLSALLEEFFIKYNTIIIKDNYSNIHGEHFIKTVLVTERMSVNAFIDYCPDLVI
                     TIGKHVWSFVKYTLRDKKLKFEHWRISEDGKIVDPFNKLTSVFQCSAEFFMETVTAGV
                     NSNNNLEYYNLWKTRLSKVCYPELVYSNFYAISELVKKIPDSALVHSSILNAARLYDF
                     VSDDKALLSFANLGADGIDGCLPTFLGQAEADKTRPAYLISGDLSFFYAMNSLCGDIP
                     DNAHIFLINNHSGSEFHTNFGKFYNGSLSVDKHIAAGHISNAKDWALENNIKYITADS
                     KESLKSGLDVFINTFDSPVLFEVFTDPDTDTSVLESFYSINKVITPKMYFRKALDIIK
                     KHLHIK"
     gene            4144..4980
                     /locus_tag="IWO35_002387"
     CDS             4144..4980
                     /locus_tag="IWO35_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0317424.1"
                     /translation="MAIKSYIKRLLLYIRHGDPKINLDVSYLSEGAMLAGKTVIITGG
                     AKGLGFAIAKSFIAQGASVIITGRDMDSLNNAKCQLGDMCSIRAFDITDAKMYRTFFE
                     DAYETFGRIDILLNNAGISLHEDGFMSVTEKTWDKQMDTNLKGVYFFTQEFIKFVKEK
                     NQMQANIIMMSSERGLCGDSIPYGLSKAAINSLVVGLSKKYISEGIRVNGIAPGVTAT
                     AMTGISEKGNLYNPATIGYRYFLPEEVAQVALFLASSLSNCISGEIIACDQGQYLSRN
                     WG"
     gene            4985..>6220
                     /locus_tag="IWO35_002388"
     CDS             4985..>6220
                     /locus_tag="IWO35_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ0317425.1"
                     /translation="MGILLKKYNSLSEPAKASIWFTLCNVLQKGILFLTTPIFTRMLS
                     TAEYGKYTIFISWYSIIAIFTTLNLSAGVYNKGLIKYENKYALTSSFLGLSSFISLCA
                     LMLYLPFRTVINGLLDLSTEFVILILIESIFEPAFLLWSVKERFDYKYQKLVLITVLM
                     AVLNPGLGIILILSFGEYAIWRVRAYVVVNVVVGMFIGIGILAKGKKLFDKEYWTYAL
                     RFNLPLIPHYLSMTLLNQVDRLMINSMVGASEAAIYSIAYTISSMMNIITTAINGSFI
                     PFTYKAIKDGVYEKIKSVANQLLVLIFVLCSFTMLFGPELIWVVGGEKYMDAVWIIPP
                     VAASVFFMFLYPLFGNIEFYYEKTNYVMFASCVGSIAKIISNYVSIPRFGYISAGYTT
                     LICYMLFSFAHYMFYKKILR"
BASE COUNT         2032 a          823 c         1239 g         2126 t
ORIGIN      
        1 tatgtgattt tttggtatac ggagaaacta gtgaattatt aaaattatat ggtattcaaa
       61 gagataaccg agcatcagac tttgggaaaa ttatttatac tgaggaaaaa gagtttaata
      121 aaaagatatt tacactacta gaaggaaaac aaggtgtatc tccatatgat gtttttccag
      181 attttgatgc actatataaa aaatatatgt ttgcagaata ctatattgtg tataattatt
      241 tcctggaaaa tattgcaagt aatgaatctt atggtgactt aatggatgcc tattatagtt
      301 atttaaagaa atatgcagtt gttgctgatt cagaaaaatt gttactatat tggccgaaat
      361 atgttcaata taatgttaag ttcgaaaatg aacttgattt ctatgcgaag ttggattcaa
      421 aaaaatggat ggatatttat gctaattata caccttttag tcagtgatta tatggaaagg
      481 atgaggtgaa taatgaaaat cggaatagtg actattgtta atggggcaag ttttggtaac
      541 agattacaaa attatgcgac tcaaaaagtg ttggaaaata acggctatac agttgagaca
      601 attaataatt gttttgacaa agaaagtacc ctatccattg ttatcaaaaa cgctatttat
      661 acattattta agtttatacc gcatttcaaa aagtcaaaaa gtatctggaa aagaatgtat
      721 acattttatc aatgggacaa gcagtatata gattattctc atcgtaaaat tgatgcatct
      781 aaaaaaaaca gctttgtgag tataaagaat gattatgact atttcttggt tggtagcgat
      841 caaatttgga atccaaaata tgcagccaat aagggatttg aacttttatc atttgttcca
      901 aatgaaaaaa gagtttcatt atcaaccagt ttgggagttc catctatacc aagtgattta
      961 gaaaaattat atgtgacaga gtggaataag tttaaatcaa tatcggtacg tgaaaatcaa
     1021 gcggcaaaaa taattgagga cttgacaggc acaactgcgg ctgttcacat tgacccaact
     1081 ttaatgttgg agcgttcgga atgggaagcc tttcaaaaaa aaccagacaa tattgatgaa
     1141 aattacatct tgtcgttttt cttaactaat ccgcctaagg atactgttga tgtattagat
     1201 aaggtcgcta aagatttgaa aataggtgtt ctcaccttag atgcgtcttg ggaatatttt
     1261 caagatatta ctccgaatca ttttgtttat ttgatttcac atgcaagaat gatttataca
     1321 gattcattcc actgcacagt tttttcattg ctatttggaa aaccggttgt ggtatttgac
     1381 cgttttgata gtgaaaaaat gaattctagg ttaagtacat tgttaaataa agtaaataaa
     1441 gcacattttt gggtaggaaa aaaagctgat gaagcaatga ttcgtgcaat agcatctgaa
     1501 cctgaatatg acatagataa gctgatggta tcagagaggg aatgtgtaaa agcttatctt
     1561 aaagagtatt tacagtaaaa ttttgaaatg ggatgaaaat atggagcatt taagtgattg
     1621 ttttggtaaa atgaagattg ttacgacttc atgcgcaaga gaatatttta aggatgattt
     1681 ggataaatca gagtattcat gttttgttga ttctcgagca gctatgtttt tcgcaatggg
     1741 acaagcttta gctaaaagaa ggaatgtagt tgtaatgctt gctgatgaat tgacatcgtg
     1801 ttatacggca ctaacagaat gtgcatttca acaagttgat ttaattgtgt ttgcttttca
     1861 gacagtcaga catgaaattg atttagcatc tttgaagaat tgtggttgca atctaattaa
     1921 ctgtagcgcc caagacgata ttgatagagt tgtgtccaac actaaggggt taaatcagac
     1981 aacagtaatt aaaatcaata tgaattgtga gtcggataaa gatcatatat gtgagtatat
     2041 agacctgcca gacatacctg tttttttcca cggtagctta tactcacctg caagtaaaaa
     2101 gaaatatgag cagtatggac aggtatcgaa gtatttaggc tatctcgtgg ggggaaaaga
     2161 tgcaatactc ataatttctc acaatgagtt tttaaacgat atcagtgtat ggattaatta
     2221 ttattctttt ggagaaattc aacccaaaat tattatattt gacagtccga atgactatca
     2281 atataaatgg ctgtgtgact tagaatgcag tgtagctcat tcaaccaacc gatttttgtc
     2341 tgaggattta gccttattgc aaaataacgg aatttccatt gtgttaagag gtgataatta
     2401 tgatatcgtc agttaaaaat gttcaaattt tagtatcttt attaaaacag cataatatta
     2461 aagatatagt tatctcgcca ggtagtagag attttcaatt ggtgcatagt gtagaaacag
     2521 acagtttttt tagaacctat acggttgtag atgagcgcag tgctgccttt tttgctaatg
     2581 gactttctat ggcgattgat aagcccgtgt gtgtatgttg tacttcttca actgcctctt
     2641 caaattatat ggcggcagct aaagatgctt ttgctaatgg catccaactt gtacttctta
     2701 ctgctgatag agattaccgc cgcttatatc agatggaaga tcagatgatt gatcaggtag
     2761 atatgtatgg ggcttatgtg cgttctaacg ttgatattcc tattgttaag gattctgatg
     2821 atgaatggta tgctaccaga aaaataaatg aagcaatatt ggagttaaat catggcgtta
     2881 agggtcccgt gcagattaat tatcaattaa tggatttaag tgcgccgttt gttgagacca
     2941 tgccagagta tcgtaaaatt acaagatatc aagaggatga tttactgtca tcagtagaga
     3001 tttttaaaca gaaactatta tcaaaaaaga gaataatggt tttatgtgga cagcaggaag
     3061 aaaataattt aagtgcctta ttggaagagt tctttattaa atataataca attataatca
     3121 aggataacta ttctaatata cacggtgaac actttattaa gactgttcta gtaacagaaa
     3181 gaatgagtgt aaatgcattt attgattatt gcccagattt ggtaataact attggtaaac
     3241 atgtatggtc ttttgtgaaa tatactctta gagataaaaa gcttaaattt gagcattggc
     3301 ggatatctga ggatggtaag attgttgatc cgttcaacaa gctaacatct gtctttcagt
     3361 gctcagcgga attttttatg gagactgtca ctgctggtgt gaatagcaat aataatcttg
     3421 agtattataa tctatggaag acaagattat caaaagtttg ctatccagag ttagtctata
     3481 gcaattttta tgcaatttca gaactagtaa aaaaaattcc agatagtgca ttagttcatt
     3541 ctagtatttt aaatgcggct agattgtatg actttgtatc tgatgataaa gcattactca
     3601 gttttgctaa tttaggggct gatggtattg atggttgttt accaacattt ttaggtcagg
     3661 cagaagcaga taaaactcga cctgcgtatt taatttcagg agacttgagt ttcttttatg
     3721 ctatgaatag tttatgtggt gacattccag ataatgcaca tattttcctt attaacaatc
     3781 attctggaag tgaattccat acaaattttg gtaagtttta caacggatca ttaagtgttg
     3841 ataagcatat agctgcagga catatttcaa acgcaaagga ttgggcatta gaaaataata
     3901 taaaatacat tactgcggat tctaaggaaa gtctcaaatc ggggttggat gtatttatta
     3961 atacatttga cagtcctgtt ttatttgagg tctttacaga tccggatacg gatacgtctg
     4021 ttctggaatc tttttatagt ataaataagg taatcacacc aaaaatgtat tttagaaagg
     4081 cgttagatat tataaaaaag catttacaca ttaagtagat gtttattgcg aggtgaaaca
     4141 aatatggcaa taaaatctta tattaaacgg ttattgcttt atatccgtca tggagatccc
     4201 aaaataaatt tagatgtgag ttatttatct gaaggtgcaa tgcttgctgg taagactgtg
     4261 ataattacgg gtggtgcaaa aggacttggt tttgcaattg caaaatcatt tatagctcaa
     4321 ggcgcaagcg ttattattac aggaagagac atggattcgt tgaataatgc taaatgtcag
     4381 cttggagata tgtgttctat cagggcgttt gatataactg atgctaaaat gtatcgaacg
     4441 ttttttgaag atgcatacga gacctttgga cgtattgata tactgcttaa taatgctggg
     4501 atctcgttac acgaagatgg atttatgagt gttactgaga aaacttggga taagcaaatg
     4561 gatacaaatt tgaaaggtgt ttatttcttt acacaggaat ttattaagtt tgttaaagaa
     4621 aagaatcaaa tgcaagcaaa tattattatg atgtcctcag aaagaggctt gtgtggtgat
     4681 agtattccgt atggattatc aaaggctgca attaactctt tagttgtagg cttgtctaag
     4741 aaatatattt ctgagggaat tagggttaat ggtattgctc ctggagtaac tgcaaccgct
     4801 atgactggta tttctgaaaa gggtaatttg tataatcctg caacgatcgg ttatagatat
     4861 tttctcccag aagaagttgc tcaagttgct ttgtttttgg caagtagtct ttcaaactgt
     4921 atcagtggtg aaatcatagc ctgtgaccag gggcaatatt taagtcgaaa ttgggggtaa
     4981 taacttggga atattattaa aaaaatataa tagccttagt gagcctgcaa aagcatctat
     5041 ttggtttaca ctttgtaatg tgttgcaaaa aggaattcta tttttgacta cacctatttt
     5101 tacaagaatg ctttcaactg cagaatatgg taagtatacg atttttatat cgtggtattc
     5161 tattatagca attttcacaa cgttgaattt atctgcaggt gtctataata aaggactaat
     5221 taaatacgag aataaatatg cattaacctc gtcattttta ggattatcgt catttatttc
     5281 cctttgtgca ttgatgctat accttccatt tagaacggtt ataaatgggc tactggattt
     5341 atcgacggag tttgtcatat tgattcttat tgaatcaata tttgagccgg cttttttgct
     5401 ttggtcagtt aaggaacgat ttgattacaa gtatcaaaaa ctagtactca tcacagttct
     5461 tatggctgtt ttaaatccag gtctcggtat tatattaatc ttatcatttg gggagtatgc
     5521 tatttggaga gtacgagctt atgtcgtggt aaatgttgta gtggggatgt ttattggcat
     5581 aggaatactg gctaagggta agaaattatt tgataaagag tactggacat atgcgctcag
     5641 atttaatttg ccattaatac cacattattt atcaatgaca ttgctgaatc aggttgatcg
     5701 ccttatgata aatagtatgg ttggagctag cgaggctgcg atttatagta tcgcttacac
     5761 tatatctagt atgatgaata tcataacaac agctataaac ggttctttta ttccttttac
     5821 atacaaagca attaaagatg gcgtgtatga aaaaattaaa tccgttgcta atcaactatt
     5881 agtgttgatt tttgtcttgt gttcgtttac tatgctgttc gggccagaat tgatttgggt
     5941 tgtcggagga gaaaaatata tggatgctgt ttggattatt cctccagttg cggcatcagt
     6001 atttttcatg tttttatatc cgctttttgg caatattgaa ttttactatg aaaagacaaa
     6061 ctacgtaatg tttgcatctt gtgtgggctc tatcgcaaaa attatttcaa actatgttag
     6121 tattccccgt tttggatata tttcggctgg ttacacgaca ttgatttgct atatgttgtt
     6181 ttcctttgcc cactacatgt tttataaaaa aattcttaga
//
LOCUS       DACECZ010000128         6169 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.131, whole genome shotgun sequence.
ACCESSION   DACECZ010000128 DACECZ010000000
VERSION     DACECZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.131"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(129..776)
                     /locus_tag="IWO35_002389"
     CDS             complement(129..776)
                     /locus_tag="IWO35_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAQ0317426.1"
                     /translation="MEQTIKPYQYFFIIREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(1184..1687)
                     /locus_tag="IWO35_002390"
     CDS             complement(1184..1687)
                     /locus_tag="IWO35_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0317427.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(1810..2574)
                     /locus_tag="IWO35_002391"
     CDS             complement(1810..2574)
                     /locus_tag="IWO35_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ0317428.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            2682..2957
                     /locus_tag="IWO35_002392"
     CDS             2682..2957
                     /locus_tag="IWO35_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ0317429.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            complement(3158..3505)
                     /locus_tag="IWO35_002393"
     CDS             complement(3158..3505)
                     /locus_tag="IWO35_002393"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317430.1"
                     /translation="MIPSVSFLFKKLKTFFILPEIEESNELTASDRSAFLLPVFNTWG
                     KAPQKQPYRRCSKVSFLVLRQIYAKMWKAIFTKFSLILKTKRLFHNQSLMLDADQLFH
                     NLIDGVLSAASRY"
     gene            3673..4602
                     /gene="yidC"
                     /locus_tag="IWO35_002394"
     CDS             3673..4602
                     /gene="yidC"
                     /locus_tag="IWO35_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0317431.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQPKNSTNNKGRNAGKQQKRK"
     gene            4705..6053
                     /locus_tag="IWO35_002395"
                     /pseudo
     CDS             4705..6053
                     /locus_tag="IWO35_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT         1925 a         1178 c         1131 g         1935 t
ORIGIN      
        1 aagaaagggg ctgtccccag actttagtag gcgtcattag ctatctttcg ttagcattta
       61 aaggcgaaca ccatcaccta ttcacttcca ttaaaagata gcatattttt ctgtaaaaaa
      121 caataaaatt atcgttcttt ctttacacgt tcagcaaatt catagaaagg agccaatttc
      181 gttaactgtt gattacattg gtcactatca tgacaaatat aattcccctt tttcgtatac
      241 gtgccatcag aacctgcttt cgttgtagaa agaaaaagcg aaacattcga tgttttctga
      301 cagattgcac agattccctt atttatcgtt ggtgataaag taccgctaat cccttttaat
      361 ttttcatctt cgtaatagac catgaatttt ttctgcgtac ctggatcatt ccagccgatg
      421 tacgtatgtt cttgcaagtc gatttcattc caagctggtt gtttgagttt tttcactttt
      481 cggaataatt tctcgatttg tggtttgcta ggctgcataa acggctgaac gatttgcttc
      541 agctcctcca atgctctttc tgcttgcgct tttgtcagtt ttacgtctaa aactgtcgtt
      601 aaaaatgtat cgatttctgg cagttcgtga tctaatatgt ctcgaatctt ttcagctgct
      661 agtgcctgaa ctgtttgtac cgtttttgga tcattgacag atgagtacgc atttaataac
      721 tgttctactt ctcttattat aaaaaaatat tggtatggtt ttattgtttg ttccatgatt
      781 ttaaagtcct ttcttctaac caaagtgcat actttgtaga aaaaagtgat cttctgcaaa
      841 gtatgtgtta cctgaagatc ctggactctt ttaaataaga tacgttttgc atcttatcat
      901 cacccccttt aatgcttatt attctatgta catgattcat tcgataaata aaagtaaacc
      961 gtatcttgaa cagatagtca actgcaaaca gaaaaaagga ctgtgacaaa agtcgtgtca
     1021 aagtcccttg tttcgaatga atggtgttca aaagcttgca tctgcagtaa taagccgaaa
     1081 cttcacaaaa atttgagaag taattttcgt aaattccttc ttatttcttg aagctgacca
     1141 cttctgtcac aacctcaata tggtctatta ttggttttac ctgttaaatc gaggattctt
     1201 tcccaaagta acgttgccat ctcttacgca tacttgcaga catgggctga gccgcttctt
     1261 tgactgcgca gtatttgtcc acgaatgtca caatatatcc ttctttatac ttaggcggtg
     1321 cgatcgttgc accccacatg tgcttcaaaa tgatatctcg ctcaagatcc gacaaatcgg
     1381 tgattttttc tgcattcttt acagcaattc ttggatggat atacgcatgt gtcccctcgt
     1441 caaatttcgt cgtacgccaa tcatagtaga acagatcatg caaaagtcca gcacgcgctg
     1501 ttgctttagc attgccaccc catttttttg cgagcagata actatagtaa gaaacactga
     1561 tggaatgctc caaacgagtg gaatgaacat gctgtgtgta attcgctaat tttttcactg
     1621 cttctgtttc taaaagatct tgtacataag acatatattc ttcgtcttca cgccattttt
     1681 ctgtcataat tatcgatctg ccaccttttt gaatttcatt ttcttctgaa attgatctaa
     1741 gtataccacc agtgttcgga ttattctagt cgctctagat aaaatagcta aagcttaata
     1801 gttccttaat cataaagaaa aatgataaag aacgatccct gctgcgactc cgacattcaa
     1861 tgattccgca tttcctttca ttgggatata tacgttctga tcagtcaaag acaagatttc
     1921 ctgactgact ccctgacctt cgtttcccat gattatagcg aaatcctctg ttttttctac
     1981 tttgtctaat gcatgtgctt cttcatttaa ttctgtaccg tacaccgtaa accctttgcc
     2041 tttcattaca gggattacat ctgccaaatc tttttgtaac acaggcaaat gataattgct
     2101 tccctgcatg cttcgcagca cctttgtact ataaatatct gccgttcctt tacctaataa
     2161 aacaccagcc ataccaaacg catctgccgt acggatcatc gttcccacat ttccaggatc
     2221 ctgtacatta tctagtaaaa gccagcctcc tgaaaaatct ggcgtatctt cagatgttaa
     2281 cgtcatcact gctaagatcc cttgcggtgt aggtagatct gacatagcgg tcatgacctc
     2341 atctgaaact agaaaagtag aaatctcttt gtttacagcc agccagtctc cccattcttt
     2401 catacctctc tgtgtcacaa aaagttcttt tatttctgca tcagacgacg cagcttcttc
     2461 gatcagatgg aacccttcca aaagatattc tgccttttct tctctgtatt tctttttatg
     2521 tagttttttt aattctttga ttcttggatt ttttgctgat tggatctctt tcacattcct
     2581 tcacctcacc tgattatatc atggaatagg tgcttgagct tgtttcatcc taaaaaaatc
     2641 ggtatcattg atttataaaa caggaaagaa aggtgacgaa catgcgcaaa gttaaaatga
     2701 atgtccaagg acgtgtccag ggcgtgggat ttcgctatat gaccaagatg gttgctgatc
     2761 agttaggcgt gaccggaacg gtaaaaaacg aagacgacgg ctcagtcaca atcgaagcaa
     2821 tcggaaatga cgacatcatc caaaaattta tcgaagaagt caagaagtct ccgagcccaa
     2881 gtggacgagt gcagtatgtg gatgtccaag aagatccgat gattgaggaa cgaaagaaat
     2941 ttgatgtggt gggataaggt tgtgaaaagg tcgcttagtt ttgagtatta aggaaaattt
     3001 tgtgaaaatg gctttccaca tttttgcata aatttgactt aataccgaga aactgacttt
     3061 tgaacaccgt cgataaggtt gtttttgaag cgctttgcct cgagtattaa ggagaaacat
     3121 tttcaaaata gcttctcata ttttgaaatg taatgactta ataccgagag gcagctgata
     3181 aaacaccgtc aataaggttg tgaaaaagct ggtctgcatc aagcattaag gactggttgt
     3241 gaaaaagtcg cttagttttg agtattaagg aaaattttgt gaaaatggct ttccacattt
     3301 ttgcataaat ttgtcttaat accaagaaac tgacttttga acaccgtcga taaggttgtt
     3361 tttgaggcgc tttgccccaa gtattaaaga caggtagtaa aaaagctgat ctatcacttg
     3421 cagtgagttc atttgactcc tctatttcgg gcagaataaa aaaagttttt agttttttaa
     3481 ataagaaaga aacacttgga atcactcttt tctttccaag tatttctttc ttgatttttt
     3541 gtcttttttc aaaatttttc gctacgaaaa agaaagaata tttatatgaa atttctatga
     3601 agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa attatagaag
     3661 aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact tgtcagtatc
     3721 ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga cacttcagga
     3781 atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct agttgagaat
     3841 ttcaattggt cttacggctg ggcagttatt gcaatgactg tgatcgtccg tatcatcatc
     3901 ttgccactag gtatcagtca atcaaaaaaa acaatgatcc aatctgaaaa aatgcaagcg
     3961 ttaaaaccac aagttgaagc agcacaacaa aaactaaaag ctgcaacaac acgtgaagaa
     4021 caaatggcag cacaagcaga aatgcaacaa gtatatcgcg aaaacggatt gagtatgacc
     4081 ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc cttgtatttt
     4141 acggctcgct acacagaagg tatccgtgaa tcatcttttt acggcatcga tctaggctca
     4201 ccaagtatgg tgcttgttgc cattgcaggg gtcgcttatc tgcttcaagg atatatttca
     4261 acaatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctgat cgtctctcct
     4321 gctatgatcg ttttcatgtc gatcagtgcg ccagccggtg ttacacttta ctgggtagtg
     4381 ggtggtgtgt tcagttgtct gcaaacattc atcacaaacg ttatgatgaa accacgtttg
     4441 aaagcacaag tggcagaaga gttgaaacaa aatccgccaa aacaagtcgt cactccaaga
     4501 aaagacgtga cagaatcggc aaagccagct gacaataaac aaccaaaaaa cagtacaaac
     4561 aacaaaggcc gcaacgcagg aaagcagcaa aagcggaaat aaaaatatat gacaaaaaac
     4621 tgtagacaaa taaaaaaatt tctttgttct acagtttttt tgttacaatc aaagaaaacg
     4681 tcgcttttcg aggaggaatg tataatgatg tccaaataat caatggatag tcgaatggaa
     4741 gaagccaaaa tttcgctaga tgcacttgtt cctaaaaatc atttggtcca taaaattgac
     4801 aaaatattgt tatttgattt tatttatcca attgttgaat caacttactc gaccattggt
     4861 cgtcctagcg ttgatcctgt catcttagta aaattagtat tcattcaata tttatttagg
     4921 attcgttcga tgcgtcagtc cattaaagaa gctgaaacaa acctagctta tcgttggttt
     4981 cttgggctta gtttaatcga taaaattcct catttttcaa cgtttggtaa aaactatgta
     5041 cggcatttca gagaaaccga ttgtttgaac agatatttat cattatcctt gaacaagcag
     5101 ttgaagctag atttattcat gaagaaattt tgtatatgga ttcaacctat attaaagcga
     5161 atgcgaaaaa acatattttt acaagagaaa tggtacataa ataagccatt ttataccaaa
     5221 aacaattgaa agaaaaaatt aatgaagcaa gaattgcaga aggcaaacgt ccttttggtt
     5281 caatcattca tcaggaggtg gtagaaggga aaattagtgt cgcagatcca gaaagtggtt
     5341 attatgtaaa aacagaacaa gaaaaacagt ttgcttattc tgctcatacc gtttgtgatg
     5401 aaaatggttt tgtattagat gtcatgatta cgcctggaaa tctacatgac agccgaatgc
     5461 tcgtgccaca aatcgaacgt gttaaatcat gttgtggtgt agctgctgat gctgcgtaca
     5521 aaacaccttg gaacgccaag tatttaatcg atcgaaaact acgacccatt tttccttata
     5581 ctcgacccaa aagaagcaaa gaacgtttta aaaagaaaga tttttattat gatccctatt
     5641 ataactggta cctatgccca aatgatcaaa cacttcagtt tcgcacaact actcgagatg
     5701 gttataaaaa atatgtatct aaatctttta tttgtgaaag ttgtctatta ttgaaaaatt
     5761 atacagaaag ccagaaacac cagaaagaaa tacatcgaca tatttaggaa gactacttag
     5821 atgaagcaga acatttacgc catacggtgt acaatcgcga aaaatataaa aaaaagaaaa
     5881 gaaacgattg aacgcgtttt tacagacgca aaggaaaagc atggaatgcg ctacaccaaa
     5941 tatcgaggct tggaaaaaac caggtgcata cgatgcttgt ttttgccgca atgaatctgc
     6001 aaaaattagc catttggcag tgggggcata tgtctttata cccgtggtgc tagttaattt
     6061 tttcctagag gaaaatgtct aaacatgttg gtaaatcaac aaaaaaacgc atatagtagt
     6121 ctcaaaactc ccaagaaatg aggactacat atgcgttcat caaaatata
//
LOCUS       DACECZ010000129         6127 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.132, whole genome shotgun sequence.
ACCESSION   DACECZ010000129 DACECZ010000000
VERSION     DACECZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.132"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(255..542)
                     /locus_tag="IWO35_002396"
     CDS             complement(255..542)
                     /locus_tag="IWO35_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0317432.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(562..786)
                     /locus_tag="IWO35_002397"
     CDS             complement(562..786)
                     /locus_tag="IWO35_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAQ0317433.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1426..1959)
                     /locus_tag="IWO35_002398"
     CDS             complement(1426..1959)
                     /locus_tag="IWO35_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ0317434.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1980..2924)
                     /locus_tag="IWO35_002399"
     CDS             complement(1980..2924)
                     /locus_tag="IWO35_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ0317435.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3561..3647
                     /locus_tag="IWO35_002400"
     CDS             3561..3647
                     /locus_tag="IWO35_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ0317436.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3768..4457)
                     /locus_tag="IWO35_002401"
     CDS             complement(3768..4457)
                     /locus_tag="IWO35_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317437.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4464..4742)
                     /locus_tag="IWO35_002402"
     CDS             complement(4464..4742)
                     /locus_tag="IWO35_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317438.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4702..5616)
                     /locus_tag="IWO35_002403"
     CDS             complement(4702..5616)
                     /locus_tag="IWO35_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0317439.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5598..5978)
                     /locus_tag="IWO35_002404"
     CDS             complement(5598..5978)
                     /locus_tag="IWO35_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ0317440.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1625 a         1131 c         1033 g         2338 t
ORIGIN      
        1 ccccccactc tccacaatac aaaaaagact agcaaatgct agccactttt tgtaaagttt
       61 cgcttgtttc accttcggtg aaattgttgc aaatcaataa ttaggtagag ctacattttt
      121 ttcgttgttc aaagattctt atgccattta ctattaataa atctataaat atgataattt
      181 ttcggctcta taatttatgg gactgatgaa tcagaattga ttcttcagtc ccattttcat
      241 tttattccaa agaattaact ccaaatacca atagaagccc atcctctata tacttgagat
      301 tccttactaa caatttcttg taattctatt aaagatttag gtaatccaaa tttatgtttt
      361 aaaatatatc ttccaagaaa actacttaat ttcatactaa cttcaccaac actttttccc
      421 tcactgattt ctttagaata aagaaagagc ttttctttta agtcaggata gtcatctata
      481 gttgcttcat tgtatacttt actaattgca tttaaaagtt tttcttcttt agtaaaatcc
      541 attataataa cctccctacc actaccttct aggtgcccat ggaccatgat taacccaacc
      601 attaacaata atttttccaa tttctccttt agcttgattc caatctaccc aacatttttc
      661 tttgttacaa taaagaccat ttccatagta agtagctgca tcaactttta ttcctgtacc
      721 gatgccagct agacaagttc ctaatattcc tagaataaca caatgtttta atactttctt
      781 tttcatgatt gtttttcctt ttcgtttttt taaaattgct tgttatatta aataatctta
      841 gtaattttat tatataacat tttattatat aacaatgatt aacgtcataa aaagtagttt
      901 tgaattcata atcgtttaaa taaaattata agatcagtta tttaaacagc tagtagacat
      961 aatgtcgata ctaccaagta acgcctgtac caagggatcc aggcgctgat tttgtgaatt
     1021 tttattggtc taggttaaaa gctcttttta aggcttggtt ttctgcggtt gtttctttct
     1081 aattctttgt gaatattgat cggtcatttt ttaaatactt gttatttttg tttgtgtttg
     1141 ttatgctttt tacatatctc atattcattt attattcttt tctaaggtta ataataggat
     1201 aatctctttc agaaagactg ctcgtacgag cagtcttttc ttatttatca agtttttcta
     1261 caaatacttc ctcattttga ctgttgtagt aattaggatt gagttttagt tccccttttt
     1321 tatttccttt gttggccact gataataaca ttgggctaaa gcttaatatt cctagtagga
     1381 tagaaccgaa tagcaagtgt ttttttgtca taagtatttc cttcttcatt tccatagctg
     1441 ccaccatttt ttccaattct ttgtttgttc gtccattgtt tctgttgctt cagaaagagt
     1501 cgttgaacta ttctctgaag actcttccgt ataagtcaac tgcagttgct cctgaagttt
     1561 ttcgatttgt tggttggctt gcagggtgag ttgttgttgc tggtcgagta gtttttgtat
     1621 atgagaatta ttttgctctt tgagggcaag ttgttctcgt aaaaattcat tttcttcacg
     1681 caactgatct agcaacttgt ttgcaaccac gtttttgttt ggtatataag catttttatc
     1741 gttgttttgt tcatgtttat gcaacttatc tagcaacctt tgattgtcta agttgcgagc
     1801 aagttgcaat actccagaac gcaatacaac cttataacca cgctcatttt tagctatcat
     1861 ggaagaatct aatttttgga tatgtttcga tattgcttgc ctactcatct taaaaagatc
     1921 tgacaattcc tttattgtaa aggtttcttg tggttgcata ctggttgcct cctaggttgc
     1981 tactggaata aattatctaa atcagataat aaactttttt tctctttttc cgaaagtgcg
     2041 gactctgttt gtttttcaag agcttttttc tctttttcta gttgtttgat ttgttggagg
     2101 agttcaagtt ctcgtttctt ctgtttgatt ttttcgttaa ttgttgtagc gggttcttcg
     2161 tattcttttt gtttatcagc taaagccaat gctttatttt ggtcttcttt ggttggtgca
     2221 tgtttgttaa agtcgtctgc ttttttgatc tcaggtttga acgtaaattg atagccaata
     2281 acaggttttc cccgtccttt tccgtatttc tttttgattg ttagtccttt gaagattgca
     2341 gttagttctt gacgtatagg ttttataact cgtttatcta tatcaggttg cttataactt
     2401 ttaggaatat ctaataattc aagaaagtct tctttggaga aatatgcgaa acctttggtt
     2461 ctgtattgct ttaacagacg aaacatagtc tttgcatagc cactttgtag ctcgttgaat
     2521 tgttgtaaag aatagcgtac ccactcgtct aaattattta acaaaggcag tgctttttcg
     2581 taaattcgta tatctacgta aggattttct acgtccccgt tgattttaaa ttctgtaaac
     2641 attacaaaca tatcgtaaga aagcccagac tcacttctac gaccaaagcg taaagataag
     2701 attttttcat aagttgcttt taaatcttct acaaatcgta catttgcagt tgctttgtag
     2761 ttacttaaat cttttagttg gtcaaacgta aaacgaactg tcttatcccc tttttcacgc
     2821 attctagaca cgatggaaaa aaacaaattc atttcaaccg gtgtaaattt tcttaaagga
     2881 attgtattta attcatggtg gtattttaca atctcattag ccatataatc acctcaatcc
     2941 aattatgaat gacatttttt aaatgtcaag acattcatat ttgacgttca aacagacaaa
     3001 atgtccttca taaacagaca aaatgtcatt cataaacaga caaaatgtca ttcataaaca
     3061 gacaaaatgt ccttcataac attctgtatc cattggtaga gtaaggacac agcttacggt
     3121 aaagaggttt aaaaagaggt ttaaaaagag atataaaaag aggggggcta attttcccgc
     3181 ctaaaaacca cttctttttt ctttaaacag gttcgcgcat ttttcaaaac tgccaatcca
     3241 aaaccttgct tcctacggtc gccaaaaagg tgtgatcgct acgctctcac attgtactgc
     3301 gcagtcgcct aaaggctcct ttgctaaaaa catgttggta aagaggcctt tgagcggttc
     3361 tgaatgctta tctaagccgt tttaagacag tcttggataa aaatccttat ctagctttaa
     3421 agcgccttac aggcggtcag ggcaagccct aacaacccta aaataagcga tacttaaaaa
     3481 caccacacaa gtttaatttt ttaagatata ataacttgtc gcctatccga cagtcttgcg
     3541 actggaagga ggtgtatcta atgtttgaat tgttcatttg cccccttctt atagaagttt
     3601 gtgtctcttt attcagttat tggttgaata accgaaacaa gaagtaaaat caataaaggc
     3661 gacatcaacc tgaaaaaaag ccccagagaa tccgacctct ctggggcttt cgtgtatcta
     3721 atgtttgatc atttgcatgt ttagtttaac atgattgccg atcaaattca attttgctgt
     3781 tgctgatcta accattcctt gacacttttt agataattta atgccgtttc ttttttcgtt
     3841 ggattttgta tgttcgttgc ttgctgaagc agtgctttat aacgttcgta ggtaactgat
     3901 tgttgccgtt cttttcgttc ctgttcacta gttgaaaaag ggattgtatc actgaataaa
     3961 ttataacaag cttcttctgt ctgcgtatct ttaaaatatt gcacgtcttt tttggttcgc
     4021 tcatagttgt cttgaacctc tttttgctgt ttttctggca agttttgata aagacgagtg
     4081 agtttttcca tgtttttatc cgtcaaacgt ttttcttgat tcacaaaaat atcgttcatt
     4141 tcctcataac aagtggtaaa ctcttccatt ttttgtgtta acacttgctc ctttttcttt
     4201 ttatcacatg ctgcttcata cttttgagtg atatccccta ataaaatact tcttgaaata
     4261 cgtttatttc cctctaaaac gccgaataaa ccacttaaaa cgattgataa tgtaaatata
     4321 acccctggca ataaaaacct cttaaatcga attttagagc cttttaatga atatcttgtt
     4381 tgataagcct tatagctaag ccaaataatt cctaaaatac tggcaaaaat gagtagatag
     4441 ggtaaataga atgccatttc tatttattct ccccatacca gccaaaaggc taacagtgtc
     4501 atgattaagc aacataaggg ggcaagaata tccattgatc gctgcataat tacttttttc
     4561 tgttctttat ttcgctggtt taatagcgca tggtggaatt ttttgacttg tgaatcaatt
     4621 gtagtttgta ttctttcttt ggtataaggt gcaagaaaag cacgttgttt ctttatttca
     4681 ttttctatgt cttgtaacgt tttagaacga taattcttgt tggtagtctg ctcctctttc
     4741 aacgtgttgg tcatggtttc ttcgagtcgg tctaatttcc ggttgttcag tcgtaagagt
     4801 tcgttgttct cttcgtactt cttttgcagt ttcgcgaatg ctagctcgag ctcttcgtag
     4861 ctcgttggtt cgttgttgat tgtaggctcg ttctcgtctg tccagctcat gtaaaatcgt
     4921 ctccttttcg taatcctttc ctagtttgtt ggatcgtact tttttctttt tgtggatcaa
     4981 ttcgtaagta aaatttttcc cacgattcaa gcaatgaatg ccgaatgttt taagagaatc
     5041 acaaaattgg ttatacgttc gagttttttt gtttttcatt gtttgatcaa tcgcaaaccg
     5101 aatttcgtct ttccaaacag gttcattttt ttcttttaat ttgatttcag acatggttcg
     5161 tttttcgggt ttcttccctc gttcagtgat tgttaagtca tgttcaagac aaatctcgtc
     5221 atttaggtca taacatttgt ttaaaaatgc ttgaccatgg gcttgaaatt tgttcccagt
     5281 ttccagattg acggagttaa tgacaatgtg gttatgaata tggtctttgt ctgtatgtgt
     5341 gtaaatcgcg atttggtggt caggggctat ttttttcgca tactctgccc ctagttgatt
     5401 ggctttttca gcggtaattt ctcctggtcg aaaagactgg atcaataagt gtgcttgtac
     5461 acgatcgtct tttccataca gcatgcgaat ttgtttcatt tcgcttttgg cataatcaat
     5521 gtcacagttt atccctgttt tgacggttgc tcgtggttcg gcgtaattga tcgctcggct
     5581 acaagaagtg gttcggctta ctttaactgt tgccatatct tttgtagctc cttttgcatg
     5641 atttttaggt tttcggataa gcgttgggcg tcgccaggat caacgtcgtg ttggtgtgtg
     5701 ttgcaccact tggcaatttg attgatattc ccgccagctt tggctaaatc gcgcgcgatt
     5761 gcctgagcaa tgtctggcgc cactttcggt gtcaccagac gcgcgttttg tgccttggct
     5821 ttcacgaaag ccggcacact catttgcaaa gcttcagctg actgcttcag ctttgcaaat
     5881 tcggattcgc tcactcgaaa actgatttgt tttggctcgg atcgataggt atatttttta
     5941 gaatgattgc tagccaaata ttgattttgt tcgctcataa gtcccaccct ttcagtacgt
     6001 cctacggacg tatcgttcgc aataccattg tacctaaaaa ggtacaataa gtaaagctca
     6061 ctaaagctta ctaaagggct tggcaagctc tatatttgct agccaaatac ctacgtattt
     6121 ggtgcaa
//
LOCUS       DACECZ010000130         5942 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.133, whole genome shotgun sequence.
ACCESSION   DACECZ010000130 DACECZ010000000
VERSION     DACECZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.133"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(107..1405)
                     /gene="eno"
                     /locus_tag="IWO35_002405"
     CDS             complement(107..1405)
                     /gene="eno"
                     /locus_tag="IWO35_002405"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ0317441.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1521..2276)
                     /locus_tag="IWO35_002406"
     CDS             complement(1521..2276)
                     /locus_tag="IWO35_002406"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ0317442.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2380..3570)
                     /locus_tag="IWO35_002407"
     CDS             complement(2380..3570)
                     /locus_tag="IWO35_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ0317443.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(3680..4681)
                     /gene="gap"
                     /locus_tag="IWO35_002408"
     CDS             complement(3680..4681)
                     /gene="gap"
                     /locus_tag="IWO35_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0317444.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(4722..5759)
                     /locus_tag="IWO35_002409"
     CDS             complement(4722..5759)
                     /locus_tag="IWO35_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ0317445.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
BASE COUNT         1551 a         1256 c         1104 g         2031 t
ORIGIN      
        1 tctctacctg ctgatatttc agcaagtaga gagttcgatt acttaactaa gtgatcatac
       61 tcgactatct ttcaatagtc gatcatgtat cttcattaga aaagaattat ttgtttttta
      121 agttgtagaa agatttcaaa cctttgtatt ctgcaacttc accaagttgg tcttcgatac
      181 gtagtaattg gttgtattta gcaatacggt cagtacgtga tagagaacca gttttgattt
      241 ggccagcatt tgttgcaaca gcgatatcag agattgttga atcttctgtt tcaccagaac
      301 ggtgagatac aactgctgtg tagccagctt ctttagccat ttcgatagct tcgaaagttt
      361 ctgttaatgt accgatttgg ttcactttga taaggattga gttagcaatt cctttttcga
      421 taccttcaga taatttttga gtgtttgtta cgaacaagtc gtcaccaact aattgaactt
      481 tgtcgcctaa tacgtcagtt aatttcttga atccgtccca gtcgttttcg tctaatccat
      541 cttcgattga gatgattgga tatttagaaa ctaattcttc gtagaatttg atcatttcgt
      601 cagttgtttt ttcgccttcg cctgagtcag ctagtacgta aacacctttt tctttgtcgt
      661 agaattctga agaagcagca tccatagcaa gaacaacgtc tttaccaggt acatagccag
      721 ctttttcgat tgcttcgatg attacttcaa aaccttcttc gtttgaacca aggtttggag
      781 caaatccacc ttcgtcacca acagaagttg ctaatccgcg agctttcaag attgaagcta
      841 atgcgtggaa tacttcagca cccatacgta gagcttcttt gaatgttgga gcgccaacag
      901 gcatgatcat gaattcttgg aagtcgattg agttatcagc atgtgatccg ccgttgatga
      961 tgttcatcat tggtgttggc aatacttttg tgttgaatcc gcctaggtag tggtataaag
     1021 gtacttctag gtagtcagca gctgcacgtg caacagcgat agaaacacct aaaatagcgt
     1081 tcgcacctaa tttacctttg ttaggagttc cgtctaaagc gatcatagct ttgtcgatag
     1141 ccatttggtc acgaacatcg tagccaatga ttgcttcagc aatgatgttg tttacgttgt
     1201 cgacagcttt agttacacct ttaccgccat aacgagcttt gtctccgtca cgtaattcaa
     1261 ctgcttcgta ttcaccagtt gaagcacctg aaggaaccat tccgcggcca aaagctccgc
     1321 tttctgtgta tacttctact tcgattgttg ggttaccacg tgagtctaag atctcgcgag
     1381 cgtaaacatc agtaataatt gacatgtttt gtctctcctt tgagtttgtt ttttcctaag
     1441 ggatttccct catatacgat tttagttaat tttgctcatt agtgcaaaca tttcttgcat
     1501 ttttcgcaaa ttttcaacaa ttattttaca gcgtctaaca atgacaaaaa tgaatctgct
     1561 tgcaagcttg cgccaccgac taaagcaccg tcaacatttt cttttgccat gtattcagca
     1621 atattttctg gttttacaga accgccgtat tggatacgaa ctgcttctga tacttctttt
     1681 ccataaagtt tttcaactgt ttgacgtaca actccacaaa tttcgtcagc gatttgtgcg
     1741 tcagctgatt taccagtacc gattgcccag attggttcat aagcaataac catgtttgac
     1801 acttgttcag ctgaaagatc agctaaacca gctgtaattt gtccttcgat ccattcagca
     1861 gttttgcctg cttcataggt ttctaaagat tcgccgcagc ataggattgg tgtcatacca
     1921 tttgcaaaga tagcttttgc ttttttattg atgtcttcgt ctgtttcatg gaaatattca
     1981 cgacgttctg aatgtccgat gatcacataa tgaacaccta gatcagcaag agctgctggt
     2041 gaagtttcac cagtgaacgc acctgagttt tcccagtagc agttttgtgc tgagattttt
     2101 agttctgtac cttcagctga tgtcattaat tcttgtaaga ataatgcagg agatccgatg
     2161 actgaatcaa ctttgtcatt tgaagggatt ttgtttttta ctgcttcagc aaattctttt
     2221 gcttctgatg cagttttgtt cattttccag ttacctgcga taataggttt acgcattgag
     2281 gaacacactc cttaatttat tgaaaaatgg ctagggagtc atgaaaactc ccttcacctt
     2341 ttgatcgatt actcgtcaga attgagcaaa cttggtcgtt tatttatcgt tgattgctgc
     2401 tagtcctggt aattctttac cttcaagcaa ttccaagctt gcgccaccac ctgttgagat
     2461 gtgtgtgaat ttgtcagcaa atcctaattg ttctgctgct gcagctgagt ctccgccacc
     2521 gatgattgtt gttgcgcctt ctaagttagc gattgcttca caaacaccga ttgtaccttt
     2581 agcaaagtta ctcatttcaa atacacccat aggtccgttc caaacaactg tttttgcacc
     2641 ttgtaatgta tcagcaaata attttactga tgctgggccg atgtctaaag ccatcaaacc
     2701 atcaggaatg tctccttcga ttacttgtgt ttcaacatca tttgaaaatt ctggtgcaca
     2761 gacattgtcg attggcaaga caagtttgtc gcctgctttt tcgattaatt cttttgctag
     2821 ttccacttta tcagcttcta ctagagagtt accgatcttg attccttttg cttcgtagaa
     2881 tgtgtaagtc attccgccac cgatcaagat tttatctgct ttaggaatca agttttcgat
     2941 tacgccgatt ttatcagaaa cttttgcgcc accaaggatt gcaatcattg gtcgttttgg
     3001 ttcttctact gcttcaccga tgaacttgat ttctttttcc atcaagaatc cagcaactgt
     3061 tgggatacca gttgaagcga tacctacgtt agaagcatgt gcacggtgag ctgtaccgaa
     3121 agcatcgtta acaaatacat cgcctaatga agcccagtat ttacctagtt ctgtatcatt
     3181 tttgctttct tttttaccgt caatatcttc aaatcgtgta ttttcgaata cgacaacgtc
     3241 gccgtcattc atgttgcgga ttgcttcttc taattgttct ccgcgagttt caggtacgaa
     3301 tgttacttct ttacctaata attcgcctag acgttttgca actggagcta aagattttcc
     3361 ttctttatct tcttctgttt ttacacgtcc aaggtgagag aaaaggattg cttttccgcc
     3421 ttgttctaga acgtagttga ttgttggcaa tgctgctttg atacgagtgt cgtcagtgat
     3481 cacgccgtct ttcaaaggaa cgttgaagtc aacacggaca agaacttttt tgtcttttaa
     3541 atcgatatct ttcactgttt tcttagccat tttatttcct cctgaataaa cttattatca
     3601 aaaaaagcgg aagaagcgcg attgcttccc cgcttttaaa tgtacatgtc tataatttac
     3661 acttgtacgt ttgtctatct tataagttag cgaagtattc taaagtacgt actaattgtg
     3721 cagtgtatga catttcgttg tcgtaccaag caacagtttt aactaattgt ttgtcgccaa
     3781 ctgtcattac tttagtttgt gtagcatcaa ataatgaacc gtaagtcata ccaacgatat
     3841 ctgaagaaac gatttcgtct gtgttgtatc cgtaagattc gtttgaagct tctttcattg
     3901 ctgcattgat ttcgtcaaca gttacttctt tttcaagaac tgttactaat tctgttaatg
     3961 aacctgttgg tacaggaaca cgttgagcag ctccgtctaa tttaccgttc aattcaggaa
     4021 ttactaaacc gatagcttta gcagcacctg ttgagttagg aacgatgttt gcagcagcag
     4081 cgcgtgcacg gcggaagtca cctttaggat gaggtccgtc tagagtcatt tggtcacctg
     4141 tgtaagcgtg gatagttgtc attaatcctt caacaacacc aaatttgtcg ttcaatgttt
     4201 tagccatagg agctaaacag ttagtagtac atgaagcacc tgagataact gtttctttac
     4261 cagttaatgt ttcgtggttt gtgttataaa cgattgttgg aacatcgttt ccgccaggag
     4321 cagagataac aacgcgttta gcaccagctg ttaaatgttt ttcagctgct gatttagaag
     4381 tgaagaaacc agtacattct agaacgatat ctacgcctag ttcgccccat ggtaattctt
     4441 ctgggttgcg gttagctaat actttaactt cttttccgtt tacgttgaat gaaccttcat
     4501 gaacttcaac tgttccgttg aaacgtcctt gagttgtgtc gtattttaac aagtgtgcca
     4561 acattttagc atctgtcaag tcgttgattg ctactacttc gattccatct acatcttgga
     4621 tacgacggaa tgctagacgt ccaatacgtc caaaaccatt aatacctact ttaactgtca
     4681 tttaagattt cctccttatg aaaatcaaaa aaatatttat tttaaagggt tacccctttt
     4741 aaaatctcat ttgcagcagc ttcatcagtg atgagccacg tttgttttgg tgcatttttc
     4801 atataagctc gaattgcttt agcttttgat tttccgcccg cgattgccat gatgatcggg
     4861 actttttcca aatcttgcaa ttctatgccg attctaggga ccttataaac gacttcacct
     4921 ttttcattga aaaagtagcc aaaagattcc gcaacagcat ttgcttgttt cagcatgact
     4981 aattcttttt cagtcatctt acgacgtgct gccatgtgta atgcacgccc aatactatga
     5041 ataacacaat tcgcttcact aatcaagttt aagacttctt ggatagatgg ctcattgagt
     5101 aaagaattgt aagttgccag acttaattgt tccggaacat aaagtgcacg gtggttaccg
     5161 tttgctttag cagccattct tgcactgaca gaatttgcct gtacagttat cgcttcacca
     5221 atcccgcctc ttgcagggac aaacaggtta tgccgtttct tattttccag attagacaat
     5281 tgttcggcta ccacagccat agtagttcct cccatcacag cgatgatatt ctcgccttct
     5341 ggaagtagtc ggtctagggc ttcgctcagc gcttcgccga atgcatcagc cactttggtc
     5401 tgctcttcgc tgtttcctga aacaacgata cagcgttggt taccaaaata ttctgccaac
     5461 tgttgttcgg tttgatgcgt acctaaaagc tgatccatga agttttcaag gctttgataa
     5521 acttcttcgc ccttagttgt cagcgtcata ccgctttttg aggtgtcgat caaattcaga
     5581 tttttcaaaa gatctgtttc tgttcgcaat actcgttctg tgatccccat actttccgat
     5641 aaacttcgac gtccaatcgg ctgcatccaa tagatatttc gtaaaatttt atagcgttct
     5701 tgcaacactt cgataatatc tggtgcaaca gattcaatca tttttatttc atccagcata
     5761 gcgatctcct tagttggaca ctttacgtcc aatgtagact taaaacgacc catatacgat
     5821 aaaaaaagaa attaactatt cgaatttcac atcaataaag ggaaatctca acctcacgac
     5881 tgtaagtcta acagttttgc tctcgttttt caactaaaaa gactagaaaa agtgaaatac
     5941 tt
//
LOCUS       DACECZ010000131         5918 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.134, whole genome shotgun sequence.
ACCESSION   DACECZ010000131 DACECZ010000000
VERSION     DACECZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.134"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            142..1602
                     /locus_tag="IWO35_002410"
     CDS             142..1602
                     /locus_tag="IWO35_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317446.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1823..3586
                     /locus_tag="IWO35_002411"
     CDS             1823..3586
                     /locus_tag="IWO35_002411"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ0317447.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3670..4128
                     /locus_tag="IWO35_002412"
     CDS             3670..4128
                     /locus_tag="IWO35_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ0317448.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            4237..4920
                     /locus_tag="IWO35_002413"
     CDS             4237..4920
                     /locus_tag="IWO35_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0317449.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            5067..5681
                     /locus_tag="IWO35_002414"
     CDS             5067..5681
                     /locus_tag="IWO35_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ0317450.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVLALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5697..5903
                     /locus_tag="IWO35_002415"
     CDS             5697..5903
                     /locus_tag="IWO35_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317451.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         2107 a          917 c         1198 g         1696 t
ORIGIN      
        1 tgtgagcttg tttgactttt gtcccagttt ctttttttat tggtttttga aagggctttt
       61 ctaaataaaa aggaaacaaa aatacgattt acagatatga ttaaataaaa tcataagaaa
      121 aactggaggc gtgtatcagg aatgctggaa aattacatcg agcgaaatat atttagaaaa
      181 gtgtatctgt gtgaacaatt atttgaattc caagaaatag acatcgaaca aacagctatt
      241 tctctaaggg tgactacacc aacgatcctt cacgatctag aaagtctagc cgagtgtcta
      301 gaatattgta taaaagaaca agtaagagag aagcataagt ataaactcgt attcaaacat
      361 ggtattgctc tttctgagct cacacagttt ctgtacggtc aatcttattt tttaaagttt
      421 ttaagttatt attttaatgg tatattcacg agtacagaac tggctgatct tgaatttatt
      481 tctttgagta aagtatatac aatcaaaaaa atcgtattgg atttttttaa agaaaatgat
      541 tatttgaaaa ataaagaaat cattatccca gaatttgact cgcggaacat cttgctagct
      601 ttagtacgtt atgtcaattg ggaaggctat gaaaataaaa atccgtatgt agaagaagct
      661 tgtaacgagt tgattgatta tgtagagact cattttttta aaagaagata ttcggaagat
      721 gaaaagtcct ttatcatacg agaaatagag atcgcaatcg gaagaaaggc atttcccctt
      781 ggactgaaag aagaagataa gaaaagcgca gaaacgaagc cgttatttca attagtctgt
      841 caaggattgg aagagcaaaa aggaattcta gacttacaag aagaagatgt ctattatatt
      901 ttttctttgt tcaacagtag aaattatacc aatgagaaca tggagctgtt aagaaaagat
      961 ttcgaagttg tttataaaaa ttttgttctc aatgacctat cactgaatga attgatttta
     1021 cagattatta gtatcgctga aatcgacgat gcagaaaatt ttattttcaa gcaatccttt
     1081 ttacaatttg tccgtacgtt gtgggcagat ggacaagtat ttttaccgga aaggatctat
     1141 cttctatcag aaaaagaaca acttctgtac ggaaaaattg tagaaatatt ggaagagtgg
     1201 aaaaagaaaa atcaaatcca actaagatgg aatgaaaatt tcattcgaaa attcgtcaaa
     1261 agtctttctt tgatcaacag tgaacaagcg gggagacagg aaatagaaat ttttgttgtt
     1321 tcagaaagtt cagtcaaaca atttttttat cgaacccaat ttcatttgta tgcggaagaa
     1381 ggcatcgctg aaataaatcc tattttattt cgttcattag aagaattgcc tgatgaatgt
     1441 ctatgtgcga aaaaaagagt ggttttgtgc gatgctgcta gctatcaaaa tggattagaa
     1501 actgaaaaga caaagatcta tccaatctca ttaaaaggtg caaacatcca tttgtatcat
     1561 attgctcagg aacttcacct attaaaaaaa catatttctt gatgagacct taattaggag
     1621 accttctaca aagcttttta attgtctgta gaaggttttt atttgagtcc tgattcgtgc
     1681 gaagatctac ttatataaca agctttctct gaaatttttt taaaatgcta ttgacttaga
     1741 gtcaactcta agtagtagaa tagataaagt gtaacaaatc tattttcatt aaaagaagat
     1801 agtacggagg agagaatgta ttatgagtac agtattacat gtattggatg caatcaatag
     1861 ccccgcagat gtcaaaaaat tatctgttga ggaaatgaaa cagttggcag aagagatgcg
     1921 ccaaaaaatc ttgcaaaagg acagcgatgt aggtgggcac gttggtccga atctaggtgt
     1981 agtagaatta acgattgcgt ttcattatgt atttgattca ccaaaggata aaatcgtttg
     2041 ggatgtttcc catcagtctt atggtcataa gcttttaact ggtcgaaaaa atggttttga
     2101 aaaaggacat taccgtgaag tgagcggata ttccaaccaa catgaaagta aacatgacta
     2161 ttttacagtg gggcacacat ctacatctat cagcttagca acaggtttag ctaaagcgag
     2221 agatctgaag aaagaatcag gaaatgtggt agcatttatc ggtgacggct ccctaagtgg
     2281 tggcttagct tttgaaggat tgaacaatgg tggtgtttta caatccaatc taatcgtatt
     2341 agtaaacgat aatgagatgt ctattgatga gaatcatggc agtttatata aaaacttagc
     2401 tgagcttcgt caaaccaacg gtaaaagcag caataaccta tttaaagcaa tgggatttga
     2461 ttatcgttac gtggataatg gaaacgatct ggaaacggtg atccgtttat ttgaagaagt
     2521 aaaagatatt gatcatccga ttgtacttca cgtccatacg gaaaaaggtc gcggttatca
     2581 gccggcaatc gataataaaa tgaaatatca ttggcatgta ccatttgatc tgtccaccgg
     2641 ggaggcaaag caagtaactt ctggagaaac atttaatatg atcatcatgg attatttgga
     2701 taagaaaatg caaacaagta cagctcctat tgccgcagtc aatgcagcga taccaggcgc
     2761 ctttggactg aaaacattcc aagaaaaaca tccagatcat tattttgacg tggggatcgc
     2821 tgaacagcac tctatcactt tcgcttctgc tttggcttct cagggagtac gtccagtcgt
     2881 cttccaatca agtactttct tgcaacgggc atatgatcaa ttatcacatg atttagcgat
     2941 caacgagaat ccagtgatta ttttagtcgg tggcggaacg atctcagcta gtgaccgaac
     3001 acaccaagga acatttgaca ttccttactt atcaagtatc ccgaacatca aatatcttgc
     3061 tccagcaaca aaagaggata tgatctctat gctagattgg gcgttcgaac aaacagcgca
     3121 gcctgtagca atccgaattc ctacacatgg tgtagaacat ggtacagcta tcgcaaatga
     3181 ttatagtatt ccagcctatc aaacagtaaa taatggagga aaagtagcaa ttttagcttt
     3241 aggcggtttt cttgaattag gaaaagaaac agtaaaagct tttagaaaaa aaggaatcga
     3301 agcaacgttg atcaatccag gaacgatcac agaaaatgac gtagaaacat tagataaatt
     3361 aaaagaaaca cacgaattag tcatcacatt agaagatggt agtttgtcag gaggatttgg
     3421 cgaaaatatc agtcgttatt atggtgattc ttcaatgaac gtattaaatt ttggtgcaga
     3481 gaaagaattc acggatagcg ttcctgtagg gcagctttat gaacgttatc atttaacacc
     3541 aaaacaaatc gttgaagata gtctgcgtgt cttgaatatt caataattca ttatgctcac
     3601 catccagata attgtttata ctagtatctg gatggttttt tcattataaa aagcaaaggg
     3661 gtggatgtta tgtatcagca agtagaagaa tatgtcaaaa agttccaaga aaaatatcct
     3721 agttatcaat cagaaaaatt aggtttcttt tcatttgggg atacagaaaa aatggcagac
     3781 gagttgagcg aactagttgt aaaaaagata aagactggta catcaagtgg ttacgatctg
     3841 tataaacaag agaatgagcc tttaccaaaa gttggtcaat tagacgtggt cctgaatgga
     3901 aaaaatgaac cagtttgtat catcaaaaat ataaatgtga ccatcttgcc ttttagtgaa
     3961 gtcacgaaag agcatgcttg gaaagaagga gaaggggatc ggacattagc ttattggcgg
     4021 aaagtccatg ctgagttttt taaacatgct tataaagaag cgttgaacat agaatttaca
     4081 gaagagaagc tggtcgtata cgaagaattt gaagtaattt tcttttagta agaaaggtct
     4141 gctgaaagaa atagctcatt attgtctcct gaatagaaaa gggttacatt tttatatgaa
     4201 gctgtatttt tatataacat tactggagga gatattgtgg atacactgga acaaatcaaa
     4261 ggcggagtcg tcgtttcttg tcaagctttg gaaaacgagc cgctacacag ttcttttatt
     4321 atggggagaa tggctttggc tgcaaaagaa ggcggagcag ttggtattcg agcaaatacg
     4381 acagaagata tcaatgaaat caagcgaatc gttgatttac cggtaatagg gattatcaaa
     4441 agagattacg atgggtcaga agtgtttatt actgcgacag aaaaagaagt ggaagaactt
     4501 ttggagacgc aagcagaaat cattgcactg gatgcaactt ctcgtaaacg tccgacagaa
     4561 aaaacaactg ctgaacttgt ccaaatgatt cacgaacacg gtcggttagc tatggcagat
     4621 atttcgactt ttgaggaagc gcttcaagcg caagaagatg gttttgatct tttgtctacg
     4681 actttagcag gctatacaga atattcacga aagacgcaga cacctgattt tgatttattg
     4741 aatcaaatca tagaacaagt cgatatccca gtaattatgg aaggacatac tgactctcct
     4801 gaacaagtag agaaagcata tgaattaggt gctttttcag tcgtagttgg atctattatc
     4861 acccgtccgc aattgatcac taaaagatat gtcgaagccg caagtaaagc aaataactaa
     4921 aaaacacgca cggcacagat tcttgacaaa aaagaaaaat tgatagatag tttaaagcag
     4981 attctaagaa gatgaaaaaa gcattacttt tcattttgtc ttcaagatct gctttttaac
     5041 gaattttata gaaagaaggc gaaaccatgt ttgaacggag tccttcagaa ttgacagaac
     5101 gtgaaaatta caagttgctg attggttcag tcatccctag accagtagca gtagtaacga
     5161 ctaaatcagc aaaagatata gtgaatatcg cacccttcag ttacttcaat atcgtcagtt
     5221 cgaatcgacc catcttatcc ttggcgatcc agagaaaaca agatgcagta aaagacacag
     5281 caaagaatat tcttgagaca agagaagctg ttatccatat ccttgatgag gacaacgtag
     5341 aagaagtaaa taaaacagca gccaatctgc ctaatgagga aagcgaactg tacctgactg
     5401 acttgacttt aactgattca gtaatcattt cagtactagc attgacagaa gcaaaagttc
     5461 gttttgaaac atccttatat gaacatgtcg agatacggaa taaaaataaa gtaacagctg
     5521 atttattttt gctagaagta cagaaatatc atttaaatga aacagtctat gatgataaga
     5581 caggaaggat cgatccaaga aaattgaatg ctgtaagccg tttggcagga aatgattatg
     5641 cagcaatagg agaaattttc acgattgcac gtcccgaata aaaaggagca ctagttatgt
     5701 taaaaataag ctataaacca tcgacagatt caaaagaaat gaaaaaagaa tatgaaacag
     5761 tgaatgattt tctgcaaggg cagtacttag aagttccgcc attgcaagac cattttgtcg
     5821 ttactactgt tactttagat ggtaaggaaa tagaaatgcc agatcaaacg attagcggat
     5881 tgtttaatta ctttaacaaa taaatattta ttccgaaa
//
LOCUS       DACECZ010000132         5910 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.135, whole genome shotgun sequence.
ACCESSION   DACECZ010000132 DACECZ010000000
VERSION     DACECZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.135"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(368..1486)
                     /locus_tag="IWO35_002416"
     CDS             complement(368..1486)
                     /locus_tag="IWO35_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317452.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1957..3264
                     /locus_tag="IWO35_002417"
     CDS             1957..3264
                     /locus_tag="IWO35_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ0317453.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3383..4582
                     /locus_tag="IWO35_002418"
     CDS             3383..4582
                     /locus_tag="IWO35_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0317454.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4609..5877)
                     /locus_tag="IWO35_002419"
     CDS             complement(4609..5877)
                     /locus_tag="IWO35_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ0317455.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
BASE COUNT         1814 a         1252 c         1058 g         1786 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcaacactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacatcaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa
     1561 taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa
     1621 gttatcttca taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg
     1681 ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
     1741 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
     1801 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc
     1861 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
     1921 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
     1981 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
     2041 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
     2101 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
     2161 atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat
     2221 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
     2281 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
     2341 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga agctaaattc
     2401 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac aatcgttaat
     2461 gcatcaggat tgaacaactc ttatcttggc gaaaaccgac cagaaggcac tggagaaaac
     2521 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
     2581 ttcccagaaa tactggatgt atcaagtact acgacccaaa tgtttggcga aaatacgcaa
     2641 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
     2701 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
     2761 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
     2821 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
     2881 tcagaaaaag agctcggaaa agctaacgca agtattccta gtctaaaaac cattgatgta
     2941 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
     3001 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
     3061 actgacaatg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
     3121 accttggcag acgataaatt aggctattta gaagacaaca atgcaccatc aacagagatc
     3181 attacggata aaacagtgga aaaagcgaat atattcgtga ttggctggcg acgcgtcgca
     3241 gaatttttcg gcaacttgtt ttaattgaac aataagaaaa acatctttcc atcggaaaga
     3301 tgtttttttg ctttttctag tttaaataac agcttttttc atctattttc gttatactgt
     3361 ataagaaaaa ggagttgaat ttatgaatct aaccagagat ctgatctttt tagttctcta
     3421 tcttattggg gtattctcta tcgtttattg gcgaaaacag aagaaggatc cttccttgtt
     3481 cttttacggc agtacgtgga catttacggt aatcttaact tattttgtcg tgttggaaat
     3541 tttttattcg accttttatt ttttgattcc attgttgttc tttagtatct ttgctttttc
     3601 ttatttcaca gaaaaacgaa ggctactgaa tggtctttta ttcaatgttt tactcatcag
     3661 tttcggcatc tacttgtttg tcttgctgta tgaaacgcaa aatatttttc ttggcggact
     3721 gatcgccttg atcacgattc cattgctact tgtcctttta tttggtattt atggattgat
     3781 tgtttttctt ttttggaacg gggtcacagt tttacgtaga gaatcgcatt cattagctaa
     3841 tcttttaacg ttgatactag ccatttttct tacactgttt cttgtttttg actttttctt
     3901 gttaaaatat ttgccgcaat ggataaatgc tttgtttttc tgcgttcccc tgatcttgat
     3961 ctatctattt atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg
     4021 gcctcgatac aatcaagact tcatcgttgt gttaggcgct ggattgatca atggagaaac
     4081 agtttctcct cttctagcca agcgaatcaa taaggcaatt gctttttatc gggctcaaag
     4141 ccgtgctaca ttaaatccgc ccatcttatt gatgtctggc gggcaaggag cagacgaaaa
     4201 agtaccagag gccattgcga tgaaacaata tgccatggaa caaggaatcc ctgaaagaga
     4261 cattcttgtc gaaaccaatt cgacaaccac tttggaaaac atgctctatt caaaggaaat
     4321 catggatcag caaatgaaag ggccataccg cgcgatcttt agttccaaca attaccacat
     4381 ttttagagcc ggtctttatg cgcggcaagc caaattgaaa gcaaatggta ttggtgcaaa
     4441 aacagctttc tactacttgc ctaatgcgtt tttaagagaa tacctcgcca tcttgatgat
     4501 gaacaaaaaa agacatatga tcatttgcgg attgcttttc gcaggctccg cattcttatc
     4561 gctgattact ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt
     4621 tttttctttc attctcttaa ctattggcaa aaagagtatc ccttttttga ccgctacttg
     4681 ctgagcagaa taaataccgc tgttgcctga acacataaag ctgatcaagc agatcataaa
     4741 gaagtatact gccgcttgtg aaccgaacaa ttcgatcccc atcacaaagc aggcaatcgg
     4801 tgtatttgtc gcaccagaaa atactccgat aaagcccaat cctgcaagaa aaggaacaga
     4861 aacatggagc aaaggcgcta gtgctgcacc aagcgttgca ccaatttcaa acaatggtgt
     4921 cacttctccc ccttgatatc ccgcgcctaa agacaaaact gtgaagaata acttcccaat
     4981 aaaatcatag acatgtgcct gtccattaaa tgattcttga agtaacggaa gactcaatcc
     5041 gagataacgt tgtgtttgca aaatcaatgc tgctgccaca actacagctc cacctatcgt
     5101 atttcgacaa acaggatgag gaatccattg tgcatatcgc gcttttaaaa agacgatcga
     5161 acggctgaat acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa
     5221 cagcaacata ctccattcag gaatcacacc catttggtaa tgtagatgag aaacaccata
     5281 gctttctgtc atgaaatttg caaacagtgc agcaaagaag ctagggaaga tcgcttctgt
     5341 tcggactttc ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc
     5401 aaatacagaa ctaaaacccg cactgattcc actgataatc aagatttccc gttctgattt
     5461 atccaatcgg aacaaacgtg ccgtattatc tgctaacgct ccccccattt ggactgctgt
     5521 tccttcacgg ccaacagatc ccccaaataa atgggtagtt atggttccaa acagtgtaag
     5581 tggaataagc cgcaacggga ttttttcttc tccaccattt ccttgatcga tcactaagtt
     5641 gtttccacga ctagaaagtc cgccgtgata cgcatagaga taagcaaaaa gcgcaccact
     5701 aactggtagt agacaaagga gccaaggatg tgatagtcgg atatctgtca caactgtcag
     5761 gctatttaag aaaaaagcag acaagctccc catgaatata ccgagaatcg ctgtgatcac
     5821 cagccacttc agcatataca ctaaaatgcg tactggactc ggtaattgtt tttccataaa
     5881 aaattcctcc tcaataaaaa aacgcctact
//
LOCUS       DACECZ010000133         5874 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.136, whole genome shotgun sequence.
ACCESSION   DACECZ010000133 DACECZ010000000
VERSION     DACECZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.136"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            641..1375
                     /locus_tag="IWO35_002420"
     CDS             641..1375
                     /locus_tag="IWO35_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0317456.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="IWO35_002421"
     CDS             1391..2560
                     /locus_tag="IWO35_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0317457.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="IWO35_002422"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="IWO35_002422"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0317458.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..4576
                     /locus_tag="IWO35_002423"
     CDS             3650..4576
                     /locus_tag="IWO35_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ0317459.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4595..5845
                     /locus_tag="IWO35_002424"
     CDS             4595..5845
                     /locus_tag="IWO35_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0317460.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1966 a          955 c         1203 g         1750 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacagc aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     4141 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     4201 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt tattagttcc
     4261 tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg ttgattatat
     4321 aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa cggctaatgg
     4381 aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag catcaaaagc
     4441 atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc ttccagaaaa
     4501 tatttttgct ttgggtcaag caattcgggg atatcgtcat caaattatta tgttaggaaa
     4561 atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc aaaaaatagc
     4621 tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg ctgcagttat
     4681 gccattaatc attattggtt ccttttttat gttattagca aatttcccga tagaaggatt
     4741 tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat cagattcaac
     4801 atttggtatt attggattag ctgttaccta ctctttggga cacaatctag caaagactta
     4861 tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt tacttacacc
     4921 tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta cctcgggtct
     4981 ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt ttgtacttaa
     5041 aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag ctttcgttgc
     5101 tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa tttgttataa
     5161 acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac cactatcttt
     5221 actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt tcttttggat
     5281 gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat tattgcaaaa
     5341 ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca ttataactta
     5401 tgaattctta tataactttg ttttttctgg tggtgcaggt tgtttatttg cacttgccat
     5461 tttaacattt ttcttttcaa agagtcagga aaataaaact ttaggtaagt tatcaatcgc
     5521 tccagtaagt tttcaagtag cagagcccct tctatttggg tttccaacta tattaaatat
     5581 aaaaatgtta attccatttg taactgcacc agtagtaaca acattaatta cttatttttc
     5641 aatgagtatg ggattagttg caaaacctgt aggagcaaca atcccatgga caactccacc
     5701 gataattgcc ggatttttag cttctggtgg acgaataagt ggtgcagtga ttcaagtaat
     5761 aacaattgca ataaatgttt tgatatacta tccattcttt aaattagatg ataatgccaa
     5821 gctaaaaagc gaaaaaaatg attagaacta cctatatgtt tctaactgta gtta
//
LOCUS       DACECZ010000134         5663 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.137, whole genome shotgun sequence.
ACCESSION   DACECZ010000134 DACECZ010000000
VERSION     DACECZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.137"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            88..522
                     /locus_tag="IWO35_002425"
     CDS             88..522
                     /locus_tag="IWO35_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0317461.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(600..1133)
                     /locus_tag="IWO35_002426"
     CDS             complement(600..1133)
                     /locus_tag="IWO35_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ0317462.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1227..2405)
                     /gene="mutY"
                     /locus_tag="IWO35_002427"
     CDS             complement(1227..2405)
                     /gene="mutY"
                     /locus_tag="IWO35_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ0317463.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2398..3195)
                     /gene="recX"
                     /locus_tag="IWO35_002428"
     CDS             complement(2398..3195)
                     /gene="recX"
                     /locus_tag="IWO35_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ0317464.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3302..4684
                     /gene="rlmD"
                     /locus_tag="IWO35_002429"
     CDS             3302..4684
                     /gene="rlmD"
                     /locus_tag="IWO35_002429"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0317465.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4858..5436)
                     /locus_tag="IWO35_002430"
     CDS             complement(4858..5436)
                     /locus_tag="IWO35_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ0317466.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
BASE COUNT         1680 a         1128 c         1014 g         1841 t
ORIGIN      
        1 ttattctttt tttatttttg aaaacttgcc atacttttta tttgttttat tttccagtat
       61 actatacgaa aggaaggtgg ctagaccatg aaagttgtac aaacaaaaga tacaatgagc
      121 aatatttact tagatgctgt acggatcagg catcaagttt ttgtagtcga acagggtgtg
      181 cctttgtctc gagaaataga caaagatgaa gcccattgta tccacttcgt cctgtactca
      241 gataaaaaag aaccgcaagg aactgtacgt cttttgcctt tagaaaacgg aaaaatgaaa
      301 ttacagcgga tggcgattct ttctgaatac agacaccaag ggttaggaaa aatattgatt
      361 gaagaagcag aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag
      421 tccacagctg aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa
      481 gatgctggca tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa
      541 gcaaaaaaca gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt
      601 tacttacgcg acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac
      661 gggccatctc cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag
      721 tcaatatcgt tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat
      781 agtcgtttct cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag
      841 gcctcatcgt ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct
      901 ctgatcattg ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg
      961 cgcgttaccc atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc
     1021 atagagtgtt cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg
     1081 tcgtgcttgt aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc
     1141 cttccttaag cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt
     1201 attatatcaa taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct
     1261 aactgatcta ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc
     1321 ttcaaccact tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt
     1381 ccgtaaaaaa ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa
     1441 tgtcgtgtct gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct
     1501 gctactaggt catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc
     1561 tcatactcct cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt
     1621 tggctatctc tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata
     1681 taataaacgt cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct
     1741 cgtttgttag ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt
     1801 gtacagatag ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca
     1861 tcaatgattt tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct
     1921 gcttcaatgc aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct
     1981 ggaagtccaa atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat
     2041 gagctaattt cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta
     2101 gcagcagctt ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa
     2161 agcttttctt ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga
     2221 taaaaataat cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag
     2281 atacgataag gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat
     2341 tgaataaact gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta
     2401 ttcactttcc tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt
     2461 ttgatataag ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct
     2521 tcgccatagt ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc
     2581 ttgcggaaga tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa
     2641 tgtttgttgg atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc
     2701 tttttcggct gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc
     2761 ttgttcaatg atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg
     2821 tcctttgtca cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc
     2881 gacaaggtcc aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc
     2941 cttttctttt aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt
     3001 cattgctagg tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc
     3061 ttcgtctaat tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg
     3121 taaagcttct ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct
     3181 cgtaacggtt aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat
     3241 ccccgtaatc aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact
     3301 tatggcagaa acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg
     3361 cataaatggt gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact
     3421 accaaaagaa gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga
     3481 attaataaaa gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta
     3541 tgaatgcggt ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa
     3601 ggatctgctt ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact
     3661 tttgccgacg atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt
     3721 acgaaaaaat aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca
     3781 gttagtccct ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac
     3841 tgttgttcaa ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg
     3901 aatcttccgc acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt
     3961 tattactcgc agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg
     4021 actgcccgaa gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat
     4081 gggggatgaa acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt
     4141 gactttcgat ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct
     4201 ttatggcgaa ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta
     4261 ttgcggagtt gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat
     4321 ggataccgtt cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca
     4381 caacactcga tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg
     4441 ttttcagcct gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat
     4501 ccaatcatta ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac
     4561 acttgccaga gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt
     4621 cgacatgttt cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga
     4681 ctaaatgaaa gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca
     4741 aaaatagctc ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac
     4801 actatttatt taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta
     4861 ttggatattc acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac
     4921 tgctaaaatc acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt
     4981 ttttggtgga acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt
     5041 caaaggttga ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg
     5101 atgttgtgct gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg
     5161 ccatctgtta gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat
     5221 ttttccatca atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct
     5281 tccgacattt tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg
     5341 agaagtaata taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac
     5401 taacccaata aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa
     5461 ccacattgta aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg
     5521 aagtaaaaaa ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac
     5581 aaatagaaaa catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac
     5641 aaatgttcaa ctttttaata aca
//
LOCUS       DACECZ010000135         5624 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.138, whole genome shotgun sequence.
ACCESSION   DACECZ010000135 DACECZ010000000
VERSION     DACECZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.138"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            174..1448
                     /locus_tag="IWO35_002431"
     CDS             174..1448
                     /locus_tag="IWO35_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0317467.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            1502..2929
                     /locus_tag="IWO35_002432"
     CDS             1502..2929
                     /locus_tag="IWO35_002432"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ0317468.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDHLYMKPVRELEQLRVG
                     SGVETKVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            2942..3814
                     /locus_tag="IWO35_002433"
     CDS             2942..3814
                     /locus_tag="IWO35_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0317469.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNELLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            3909..4904
                     /locus_tag="IWO35_002434"
     CDS             3909..4904
                     /locus_tag="IWO35_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0317470.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKDN"
     gene            complement(5509..>5624)
                     /locus_tag="IWO35_002435"
     CDS             complement(5509..>5624)
                     /locus_tag="IWO35_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0317471.1"
                     /translation="LNTNALINRLVHHAKIIQTTGKLYRIKDYKEKKLSYK"
BASE COUNT         1929 a          778 c         1082 g         1835 t
ORIGIN      
        1 gacacaccaa taaaagtcat atcctgtgac attatatggc attatccaaa tagaataacc
       61 ggttgacatg taagcggatt ccttttataa ttttaggagt ataagtcctt ttctaaaaat
      121 attaaccggt tatcatgaaa ggggatacaa atatatcgtt ggaggataaa aatatgtcaa
      181 aggttaacgt atttaaaaat caatcctatt tacaaagttc agctacatta ttactatttt
      241 ttgcttcttg gggtgtttgg tggtcatttt ttcaactttg gctaacatct gaatcaaatg
      301 gtttagggtt atctggcagt gctgtaggaa cagtattctc ggcaaattcg ttagttacct
      361 taattttgat gtttatttat ggaacattac aagataaatt gtatattaaa cgaaatttat
      421 taatttttgc ttctgtatta gcgacacttg ttggaccatt ttttatatgg atatacgggc
      481 cattgctaga taacaatttt aatttaggca ttattatggg agcactattt ttgtcagctg
      541 gatatttagc ttctgtagga gtttttgaag ctgtgtcaga aaggtttagt cgtttatttg
      601 gctttgaata tggacaagca agggcgtggg gatcatttgg ttatgccttg gtagcgcttt
      661 tggcaggatt tttatttgta aaaaatcctc atttaaactt ttgggcggga tctttctttg
      721 gttctttact attgttaaat ttattatttt ggaaccctaa agttgaacgg gaagcaaatc
      781 aaaattttaa tcaagaacaa gctgaatcaa atagtattcc ttctttaaaa gaaatgtttg
      841 atctaatgaa actgcctcaa ttatggacga taatcatctt tattgttttt acatggacat
      901 tttatacggt attcgatcaa caaatgtttc cgggatttta tactggtttg ttttcaacat
      961 cagctaatgg tgaaaaaata tatgggacat tgaatgctat tcaagtattt tgtgaagcgt
     1021 taatgatggg aattgttcca atcattatga gaaaattagg ggttcgaaat actttgttat
     1081 taggtgtaac cattatgtgt gtacgaattg gattgtgcgg gtttgcctcg acaccattat
     1141 ctgtttcatg cataaaaatg ttgcatgctt tagaagtacc attatttaca ttaccaatgt
     1201 ttcgctattt tacacttcat tttgatacaa agctatcagc aaccctctat atgataggat
     1261 ttcagatagc tgctcaaatt gggcaagtga ttttatcaac accattggga atattaagag
     1321 acaacgttgg ctatcaacca acatttaaaa ttatttctct tattgtatta ctagcaggca
     1381 tatatgcatt ctttattctt aaacaagatg atagagatgt tcaaggggat ccatttattc
     1441 gaggataatg gaaaccaaca gaataaaaaa caattttatc tagaaagtga gcgaatatat
     1501 tatgacaaaa agagctatag aattaacaaa tacacgttat cgtttgggat atcatgtgtc
     1561 tgcaccatca ggatggatta atgatccaaa tggattttgc tattttgatg ggtattatca
     1621 tgtgttttat caacatcatc catatagtgc tgaatgggga ccaatgcact gggcacatgc
     1681 tagaagtaag gatttagttc attgggaatc tttaccactt gctttgactc ctggagatca
     1741 agaagatgaa ggtggttgtt tttcaggaag cgctattgaa aaaaatggtg tgttgtatct
     1801 gttctataca ggacatcact attatggaga caatgatcca gatcattttt ggcaaaatca
     1861 aaacatggcg tatagtacgg atggcattca ctttactaaa tatgaaaaaa atcctgtaat
     1921 agcaaaagca ccagaagata acacacatca tttcagagat ccaaaagtat ggaaacacga
     1981 tgaaaaatat tacatggttt tgggtagtca agaaaaagat ggattaggtc gtgctattgt
     2041 ttatcagtcg ttagatttat taaattggga ttacattgga ccaatttcta aagcgaagga
     2101 agtacttaca gaaggattta tgtgggaatg cccagacttt tttgaattaa atggaaaatc
     2161 tattctttta ttatcccctc agggaattga agcaaatgga aaagattatt taaatctata
     2221 tgaaacaggc tactttatcg gtgattatga ttatgagaca gcaaaattca ttagaggaaa
     2281 tttttatgaa ttagataagg gacacgattt ctatgcaaca caaacaactt tatctccaga
     2341 tggtagacgt attgttatgg cttggatgga tatgtgggaa agccctatgc ctgaaaaagt
     2401 agacggatgg gcaggagcat taacgcttcc tcgagaatta gagttaattg gagatcattt
     2461 gtatatgaaa cctgtgagag aattagaaca acttcgagtt ggcagcggcg ttgaaacaaa
     2521 agttaatttg gcaggagaaa taataatcgc tcaagatgct tcattcaaag aagctctaat
     2581 tgatgtacta ttgactaaat caaaaaatca agaaatcaaa ttttcattaa aaacaaaaga
     2641 tgaagagtta attaatattt tttattctaa ggataaaaat gaatgtacct taactcgtaa
     2701 cggcaaaaat gatccaaggt atggaaccat tcagatgtgt gatagattat cacttcgagt
     2761 gtttatcgat aaaagttcgg cagagatttt tattaatgat ggcgagcttg tttttacaga
     2821 gcgttattat acagaaaatc aaccaagtat gcatttgagt gttagtgaag aagcttttgt
     2881 ctcgagtaca atttatcatc taaaaaaaga tgcaatttct tacgagtaag gaggttttat
     2941 gatgttaatt ggtagcatag aagcaggtgg gactaaattt gtatgtgccg tgggagacga
     3001 ggattaccga gtacaagacc aaataatttg tccaacgacc acccccaggg aaacattgaa
     3061 aaaaactgtt gactatttta aacagtttga tataaatgca ttaggtattg catcatttgg
     3121 tccaatagaa attcgaaaaa attcaccaaa atcaggatat attacgtcta caccaaaggt
     3181 gggatggcga aatgttaatg tgttaggtta tttacaagat gagctgaatg ttccaatgtc
     3241 ttggacaacg gatgtgaacg gatcagctta tggagagtac atcatgtcca ctttatcgaa
     3301 tgaaaaaata aattcattag tttattacac cgttggtact ggcgttggtg gtggagctgt
     3361 tgttgacgga aaactattag gatcgtttgg tcattcagaa atgggccata cgtttgtaaa
     3421 acgccatcct gatgatattg attttaaagg aatttgtcct tatcatggtg attgtttaga
     3481 aggcttagtt gcgggaccaa cgtttgaagc acgttttgga aaaaagggtg aagacgtttc
     3541 tttaacggat cctatttggg atgtaatggc ctattatcta gcacaagctg tgattcaaac
     3601 aacattgatt atacgaccgg ataaaatagt atttggtgga agcgtagtaa atgaaaaatt
     3661 gttagaaaaa attcgtgtta atgttaatga attattggat gattacgtgg aaataccacc
     3721 gttaagtaaa tatattacgc gtcctttaat taaaaataat ggatcggcaa cactaggaaa
     3781 ctttgctttg ggcttgaatc aattacaaga ataaaacata gaaaaaatat cgattatcag
     3841 gtatagtagt tctatagagt tctttttttc gatagttaat tattgcatta tttaggagag
     3901 acaagagaat gcgcccaaaa ttagaagatg ttgccaaaag agcaaatgta tcaaaaacaa
     3961 ctgtttcacg tgtattaaac aatcgtggct atttaagtca aaaaacgatt gataatgttt
     4021 ataaggctat agaagaactc aattatcaac cgaacgttgt agcgcgtcaa ttatttcaaa
     4081 agaaaactaa cattgtcggg ttactatttc ctacagtagc caatcctttt tttagtgaac
     4141 tcgttgaggc attggaaaaa aagttatatg aaattggtta taaggtatta ataggcaatt
     4201 cgatgaataa taaagaaaag gaaacaaatt atttaaatca gttattgtct gatcaagtag
     4261 atggtttaat tgttggtaca cataaccaag ggattcagga atataaatat cagaatttac
     4321 cgattgttgc aattgatcga gtgatgaatg aagatattcc agttgttgaa tccgataact
     4381 acaatggtgg aaaacttgca accaagttat taattgcaca aggtgcaaaa aatattattc
     4441 atacaaacgg tccaattgac ttacagactc cggctaatcg tcggagacta gcctatgaag
     4501 atactatgaa agcatatcag ttaattccac gaactgtgac attagatttc aatatttctt
     4561 atgtaaagaa aaaacagatt tttttccaaa tgtttgagga ttatcctaaa atagatggta
     4621 tttttgcttc aaatgacatc gatgcggccc taatattaca agtagcaaaa gaaaaggggt
     4681 taaatgtacc agctgattta ttagtagtcg gatatgatgg tactttaatg actcggagta
     4741 tattaccaga tctgacaaca gtaatacaac caattaatga tatagcagac acagctgttg
     4801 ctattttgat gaaacgaata aataaagaag aaacaaaaaa ggaatatatt ttacctgtca
     4861 cattatggat ggggaagaca tcagtaaaga aaaaggacaa ttaaatttat aaagactatc
     4921 aatgataatt tcattattaa ttcgtgatat aaaaagttgt taaatctctt ataatagact
     4981 atgatacaaa atcatgcttt taatctgtgt agatgttgcc aaatatttga aagacataga
     5041 acttgtcaga aaaaccttaa caattcctca atgggcggat aagctaggca aaagggctgg
     5101 cataaacttt tcagtccttc taacagaatc aattgcagat aaagcagata acattcttct
     5161 ttctggaaga aataattaga actcaaaagg gtcagaaact taaaagtttt tgatccttta
     5221 ttttcaagtc tgatttgaca gattttcggc agctcattta tttatggtca gaaatagttg
     5281 ctgaaatttt aatctattag caaatagtcc agaacgtatg aagaggattc ggatatcatt
     5341 tgtcattaac accataaata tttataaaat tcgtgttcac ctacaattaa atttaatata
     5401 taatattcgt tgtaagggct tataattttt tgttaatgaa caagatatgt aattgatagt
     5461 gtcaatagct attcaaaaat gtaggctttt ggcccataaa taacaaggtt acttatatga
     5521 taactttttt tctttataat ctttaatccg atagagcttt ccagtagttt gaataatctt
     5581 agcgtggtgc actaatcgat tgattagtgc atttgtgttc agtc
//
LOCUS       DACECZ010000136         5562 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.139, whole genome shotgun sequence.
ACCESSION   DACECZ010000136 DACECZ010000000
VERSION     DACECZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.139"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            7..648
                     /locus_tag="IWO35_002436"
     CDS             7..648
                     /locus_tag="IWO35_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317472.1"
                     /translation="MIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLSAV
                     LHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMRYQ
                     IDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIH
                     RFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            707..1147
                     /locus_tag="IWO35_002437"
     CDS             707..1147
                     /locus_tag="IWO35_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317473.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1159..1827
                     /locus_tag="IWO35_002438"
     CDS             1159..1827
                     /locus_tag="IWO35_002438"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0317474.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1891..2514
                     /locus_tag="IWO35_002439"
     CDS             1891..2514
                     /locus_tag="IWO35_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ0317475.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2570..2956
                     /locus_tag="IWO35_002440"
     CDS             2570..2956
                     /locus_tag="IWO35_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ0317476.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3169..4560
                     /locus_tag="IWO35_002441"
     CDS             3169..4560
                     /locus_tag="IWO35_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ0317477.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5171..5539
                     /locus_tag="IWO35_002442"
     CDS             5171..5539
                     /locus_tag="IWO35_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0317478.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
BASE COUNT         1840 a          923 c         1176 g         1623 t
ORIGIN      
        1 gatactctga ttgagcgatc tacatttcta tatgttcaat tgcaggaaaa taaaacattg
       61 ctgcattttt caccagagtt ccatctagag gggctaacat taggagaaat ctatcaagca
      121 gaagggctgt cggctgtttt gcactttttt aaggtagaat tggaattacc agtaaaagat
      181 tatatcgagc tttctcttga atcttgggat cgagcagtgg ttgaactgtt tccagaaggt
      241 ttaatgatcg atacaaacga tgggaagatc catctgactg cagcagaact gcaaaaacaa
      301 atgcgttatc aaatagatga cgaaaattct ttttctattt ttagacgtca gcaaaagatt
      361 ctaaaaagct ttttaaaagt gatcagtaaa aaacggaatc tgatcaaaac gacacagctg
      421 ttgaaaaaat atccaggtct gcttcacacg tctattcaat ttcctcaatt gattacgatg
      481 atccatcgtt ttatacgtat gcaacagcta ccatctaaga agctgtttct tccattgacg
      541 gacacatttc gagtagtcaa cagagaagat aaaaagaaag tgattgtgat tgactttacc
      601 agaaatcgga atgtccttca ccaagttgca atggaaaagg ccaattaagg ctttttcttt
      661 ttggattttt ttgtcataat aaatattaga tgatggagga gataatatga tgcaaacatg
      721 gttagaaagg ctaatacgat ttcagcagaa ctatcacaaa cttatgcagg gaagatatgc
      781 gaaatttgat cagttgaatc gcggtctatt gatcgctgcg cttgtcttta gtttgctaag
      841 tcgttggttg ccctatagaa tcggacaatt gttgttcatc gtctttttcg gatggatgat
      901 atttcgtttc ctatcaaaaa aaatctaccc cagactaaat gagaatcaac gttatttgaa
      961 atatcttgat agagtcaaag aaaaatatca tcagataaaa aacaaaaagc aagtcaaaat
     1021 ggatcgaaaa agttatactt tttttgagtg cccgaattgt catcaaaagc agcgtgcacc
     1081 taaaggaaaa ggacgaatac gtgtgacatg caaaacatgt ggtataaaat tcgaaacgaa
     1141 tgtttaaggg agaaaagaat gaaattagta ttattacgtc atggcgaaag cgaagcaaat
     1201 tttgaaaatt actggacagg atggctagat gtcgccttaa cagaaaaagg aaaagaacaa
     1261 gcagcagttg ccggtcaaaa aatgcttgaa gccggacttc aattcgatgc cgtttatacc
     1321 tctgttttga aacgtgcgat tcttaccgga caagtcgcac tagaagaaat gggtcagtta
     1381 tggcttccta taataaaaac atggcgtcta aatgaacggc attatgggga tcttgtgggt
     1441 aaaaacaaag ataagatgaa agaaatatac ggaaaagatc aagtaaaaac atggcgcaga
     1501 ggatatacgg aagttccgcc gttaaccgca gaaaatagat ttgaccgcag atatgatcac
     1561 ttggatccgc atttgatccc atacggagaa agtctggaaa tgacagtgaa tcggatcatt
     1621 cccttatggc aagatcatct agcaccaaaa ctaaaagatc ggcaagattt actagttgtg
     1681 ggtcatggta atagcatccg agcactgaca aaatatttag aagatgttcc tgaagatcaa
     1741 atggatacga tcgatatccc aaatgcacaa ccaatccagt atacattagc agatgatttg
     1801 acaattctgg acaaaaaaat actttaaaga acacctttat ctaaaagtga gagatagaaa
     1861 aaagaagcaa aggaatggaa caaaaggaac atgaaaaatt atatatggga ctttgatggt
     1921 acattgtttg atacgtatcc cgcaatggta gatggtgcat ggcaagcact aaaagatttt
     1981 ggtatcagta tggacaaaaa agaaatctac ttcaaaatga aaaagtattc tactagttat
     2041 ttgatcaatg aatcaaactt gaatgccaga gagtttaatg aattatttca ccgatatgaa
     2101 aaagaaagta cggaagtttc ccgtccattt ccagaaacga aacaagtact ggaaatgcta
     2161 aaagacaacg gcggtcgtca tttcatcctg acccaccgtt tgacagaatc aacatggggc
     2221 ctgttaaaag aacatcgatt ggcacatctg attgaggaag tagtagggat cgatcaggac
     2281 tttcctcgta aacctgatcc agcatcttta aattatttga tcgatacttt tcatttagaa
     2341 agaacagaca cgatgatgat cggcgatcgt cgtttagata tcgaagcagg gaaaaatgca
     2401 ggagtcgcta cttgtcttta cgacatcgat cattttctgg gtgagatccc ggcagattat
     2461 gtggtaggaa atctgaatga aatccttacc ttgcagagca tcattaaaaa gtagtatccc
     2521 ctttcaaagg agatatgtta taattttcat aaaggagtga taaaattata tggaaatcag
     2581 aagacaaaaa gaagttgtag aagcatttca ttatgatatg agattgccga accaagaagt
     2641 agaaacagat ttgaaagtag gcttctcgcc tttaaaagaa acgatcgaaa actatccgaa
     2701 agaacatagc atcgtaggag cacgcttaga gttccgttta gtgtttgata catatgtttt
     2761 atctggaagt gtgagtcaga tcaatcatat cctcaatcgg aaaattgaag gtcaaaatga
     2821 tgtcacacaa gaagaagtgg acgagctagt agcaccgtta ttcgatatcg tacgccgaat
     2881 gtcttatgag gtgacagaga tcgccttaga tcagcctggg gtaaaattga attttcaatc
     2941 agaaaaacaa gaatagacag taaaaacata cctgcgatag attaaagcta tcacaggtat
     3001 gtttttttat tactaattgt aagcaaaaag aatgtcattt cgataaaaat cgtctaaaat
     3061 ataaaagtca ctgaaaaaaa ttttttcagg aggatggttg aagaatcatt ctcgggaacc
     3121 tttacttcct tcgatggact tgtcattaat aatatgagga ggagaaaaat gacaacactc
     3181 atcacagttt tagtggttct agtttttgcg gcattattat atgtgttcta tcgtatgcaa
     3241 aagcgccata caaaattttc aacacgcgtc tttacagcat taggagttgg tatcgtcttt
     3301 ggcggtctgc ttcaattgat ctttggtgta gacgacaaag tcgttactca atcaatggaa
     3361 tggatcggga ttgtaggtca aggatatgta tcattcttgc aaatgctagt tatgccttta
     3421 gtgtttattt cgatcattgg tgcattcaca aaaatgaaag tttctgaaaa gctagggaaa
     3481 attagtgcga ctgtattgac ttctttactt ggaacaacag ctattgcagc attgatcgga
     3541 attggttcaa caatgctgtt cggactccaa ggagctaaat tcacacaagg agctgcagaa
     3601 acagcacgaa tctctgaact ggcaacacgt caggaaacag ttgaaaactt atcgattcca
     3661 cagcagattt tatcctttat ccctaaaaac gtttttgctg atttagcagg tagtcgtgca
     3721 acaagtacga ttggagtcgt tattttcgcg gcatttgtcg gaatcgctta tctaggcgtg
     3781 aaaagaaaag cgccaaaaga agcagcattc ttttcagatt tagtcgacag tctgtataaa
     3841 atcattatgc ggatcgtgac gcttgttttg cgtttgacac cttatggagt atttgctttg
     3901 atgacaaacg ttttggcaac aagtgatttt gcagctatcg tcaatttagg taaatttgtc
     3961 gttgcttctt acgtagcatt gcttgctgtt ttgattgtcc actcactgat tttgattggt
     4021 gcaaaagtca atccaatcaa ctatttcaaa aaagcctttc cggtattgag ctttgcgttt
     4081 acttcacgtt caagtgctgg agctttgcca ttgaatatcg agactcagac aaaagcttta
     4141 ggagtagatg atgcaacagc taatttctca gcaagttttg gtttgtctat tggacaaaac
     4201 ggctgtgctg gcgtttatcc agctatgttg gcaacgatcg tcgcaccagc agctggagtg
     4261 aatgtcttta gtttggaatt tattttgatg cttgtggcaa ttgtaacaat cagttctttt
     4321 ggtgtagctg gagtcggtgg gggcgcaaca tttgcttcac tgatcgtttt aggggcaatg
     4381 aacttgccag tagcaatcgt tgggttagtt atctctgttg aaccattaat cgatatggca
     4441 cgtacagcag taaacgtcag cggtagcatg gtagctggta tcgtgacaag tgcacgcatc
     4501 aaagatttag atcgcgatgt catcaatgat gattctaaag tgatcgatgt gaatgcatag
     4561 tcaaaaagag gttgtgatag aagtggtcag ctttaagaaa taagaagaag tttcctaaaa
     4621 atagtttctc aaatttttgt gaaatttcgg cttatttccg agaagctact tctgaaacac
     4681 cgtgaataag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaaacaaa
     4741 aatagttcct catattttac gtttggttga gcttattacc gcagatgcaa gcttttgaac
     4801 accgtttaag aaagaggtcg tgagcctgcc gtttcactct gagtcacaaa gagcaatttc
     4861 aaaaaatagc ttctcatgtt tgttgaatat tgtgtcttgt gaccgaggag ttgtcaggag
     4921 aacaccgttt accagaggtt gtgacttgtt tttagtcacg acctcttttt ttatttattc
     4981 catataaata cacattattt attccgaaaa gtgaaagcgc aaataacaaa aacaaccaac
     5041 ttatttatta ttaaagaaaa gcgtatttaa gtaggacgat tctgttgaga gctttcgtag
     5101 attttcccat tttcctatgg taaactgaca aagttgagaa tgttagaaca gatcgtttag
     5161 gaggaaacag atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa
     5221 aaatgcaacg ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg
     5281 gacgcttata attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg
     5341 agcatttgtt gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta
     5401 cagtaaagat atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga
     5461 tatggtacag cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt
     5521 tgcggttaca ggtaaataat agaaataaag actttgtttt tc
//
LOCUS       DACECZ010000137         5514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.140, whole genome shotgun sequence.
ACCESSION   DACECZ010000137 DACECZ010000000
VERSION     DACECZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.140"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            70..2130
                     /locus_tag="IWO35_002443"
     CDS             70..2130
                     /locus_tag="IWO35_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317479.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2213..2782
                     /locus_tag="IWO35_002444"
     CDS             2213..2782
                     /locus_tag="IWO35_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0317480.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2957..3763)
                     /locus_tag="IWO35_002445"
     CDS             complement(2957..3763)
                     /locus_tag="IWO35_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317481.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3901..5415
                     /locus_tag="IWO35_002446"
     CDS             3901..5415
                     /locus_tag="IWO35_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ0317482.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         1961 a          954 c         1125 g         1474 t
ORIGIN      
        1 atataatgaa atcatcttat gaaaattact acttcatgtt tagacaagag tagggaggac
       61 taagaataaa tgtgttcaaa aagacgttca aaacaactaa ataaacacgc ttgtcgttta
      121 gataaggaac ggtcgtgttc tgaaaaacac tacgttaagt ttatgatgcg cacgatgctg
      181 atcagtttat ctgtaggtgt ctatatgcat gtacccgttc aggcaacgac acaaaccatc
      241 aaggtcgtac gtacctctgt ttctgagacc actgattccc ctatcactat tactcatact
      301 ttcactgatg caaatggaaa acagctactt cctgcattga cttcctataa taaagtcagt
      361 tatcctccaa cgataaacca ctactggtta attcctaaca aaacattatg gacatcggaa
      421 accggaagca gtgtgccgct ttctatcttt tccgatatga ctgatcctac aaaaggtgat
      481 cagttaaaac agatcactga tattttgaat catgctttga cagatataaa ggacggtgga
      541 agcgcacaag tttcttatgt atatgaaaaa gattattcac agtttgaaag aaagaaaatc
      601 gatgttctgg cagggacaaa agtttcgata tctgatctgg tagaaaggct gaagaacgca
      661 gatggaacac aaggagatat gtccaaagta atttcaaaca atgggaatat cgtggatacg
      721 actaatacag gaacatctac tatttccttg tcctattttg atccaattgc tttaaaaaca
      781 atgactgcat ctgctgtgat ccatgttcgt gctgatcaaa cagctatcaa aggaaaagat
      841 cttcacttaa gaacaggaga gagttacgcc cctaaagatc ttgttgaata tgtaacaaaa
      901 agcgatggaa cttcaggaaa taaagaagaa atcaaaatga acgggacagt agatactaca
      961 actccgggag aatatcccgt tgtattaact tatatagact cgaaaacatc aaatgtggca
     1021 gaaacaacag ctgtagttta tgtagaagca tctgctgctt catcgactgt catcactcat
     1081 tttatagata gtcaaacaaa taaagaactt caagcagaaa cacatatgag tagtgtaaat
     1141 cgtattcctg atttgcgcct acctgctgaa ttttctgatc gagtgccaaa tatagattta
     1201 tcgacttatg aacaaaatgg gaaagtcact tctatttctg attggatgca agcaaacgga
     1261 ttagacagaa aaagtcactg gtcggagatc cttgaagtac ttcactcaga actgatgaat
     1321 aataggaagc aatcaggagg ggaaattacc tttacttatg tttatatacc agacaatcgt
     1381 tcattagaag gtcatgatct tattgttcat ccaaacgaaa aaataaacaa agaagagttg
     1441 atccaatcag caacaaattc cttaggtaaa cccgtaccgt taaaggatgt atcggttaca
     1501 atcaatgaag taccattgga ggatggttat ttgaccaaac aaacaggtga ttttacgatt
     1561 atgtatagtt atcatgaccc gtattcactc caagaactcc atgctcagtc actattacat
     1621 gttttaccat taaacaaaca gatagatcaa aaagaacaaa aaagacaaga agaaagcttg
     1681 tcagaaaaaa aacaacaaat ggctcatgca gtttctccaa cagggaaagt aaaacaaaca
     1741 agaagcctag agttagcaag aatatctaga aaaatctcag aatctaataa aacgataagg
     1801 caagaagctc atttggcccc acaagtttct ggattggaaa atgaaatttt agagcctaaa
     1861 ataaaacaac aatcagaaaa agaatttgtt gctaaacagt cggaagatca gaaggaatca
     1921 gatgaccaga aggaaacgaa gaaaaaacgc ttgtcaacta ttgattccac cgatgagcat
     1981 ctgcagccgc aagtaccact tcctagtccg ggaacacccg cacctggtgg aggtattcct
     2041 tcttctgctt taggagattt catgcgaggt atggcaggaa catgggtata cgctaatcga
     2101 aatgaatttg acgcagatga ctggttctga tttatatctc tatctgttat agtcaaataa
     2161 gtagctgagt catatgttat ttggcataat ggagacgagg agggatagaa aaatgttaga
     2221 taaaacgtta ccttacgcag aaatctggat gacaagacca cttcaacaaa atgttccaat
     2281 cacgccttta gcggaaggtt ttgaatttcg attttatcaa aaagaagatg aatctgcttg
     2341 ggcggagatt gagacttccg ttggggaatt tcaaacgaca gatgaagcac ttgagtattt
     2401 tcaacaaaac tttgctcctt atcctgaaga acttgaaaga aggatgtttt ttgtagagac
     2461 aaaaaaagga gaaaaagtag caacctgtac cgcttggcga aaaaaatcaa gagagcaagt
     2521 gtatccggtt tttcattggt tagcagtgaa accagattac caaggaaaag ggctcgcaaa
     2581 agcattgact gccagagtct tgcaagaatt tcctaccctc catacggacg gaccaatcta
     2641 tcttcacaca cagacatgga gccatcaggc gattgctctt tataaaaaaa tgggttttac
     2701 ttttattgct gaaaatctag acggcacacc taatccagat tatgaaaaag ttatgcaggt
     2761 tttaagtaaa ttagataaat aacgaaatgg tgaaataagt cctcagtatt gggataaaaa
     2821 acgaaatagt cattcctcat atcttacttt tttgagttaa tactatcgga taactaatgg
     2881 catgccatac attcaaaaaa gaaggggcag tgacaaaagt attgtcactg ccccttctct
     2941 tgttttttag catgttttat cgtttgattt ttttaggaat aaaattcttg atgaacgtaa
     3001 atttttggta atcgtttcga ctatagaggt aaccaaatac actgaaaaca actgctccaa
     3061 taaaattaac aaataaatcc ttcatagtat ccatgattcc cacgtctaag taaccatttt
     3121 ggatgattgt tttatctgtt ttaccatctg cattttgact ttcaatgatt gtccgagtga
     3181 tatctggaac atgatagaca gtgtttccgt ctttacttaa ctccacacta ttgatttgct
     3241 ggatgatcgt atctttttgc atatcgaatt gcagtaattt atctgctgaa aattccacaa
     3301 actcccacat aacaccaact gtcatcgaaa aacagaagga aacaactgct agtaataaag
     3361 gagtcaagtt catggttttt gtatttttat tgagcaaata aacaagggaa aagccgacac
     3421 ccccagctaa aaatccgctg aatgcatgta gaaaagtatc ccacattgaa aaatagccat
     3481 aaaaatcact taactctcct aaaactgttg aactaaaaat aaaagataac aaaatgattt
     3541 ctagcatatt tggaatctgt atctttaata tcttttccag aaaataaggg atttcaaata
     3601 aaattaatgt cagtaataaa agcattgtat ttccccagtg ttggttccag acctgatcca
     3661 ctagcataat aacgacgatg atacgtaata aaatatagat tccgtttgtg attctacctg
     3721 cagtttttgt actgttcgta aaaaaggttt tcaaatgatt cataaaataa ctccttcatg
     3781 tatatcctta attatactaa gaagcgtgtg tttaccaaag ctatatgact taaagaaaaa
     3841 cagagttgat attgtgaaaa aaatcactta atatagtata aggagaaaag ggggatacaa
     3901 atgaaaaaat tagttgctgg tttattaaca gccgttgcac ttcttgctat gagcgcatgc
     3961 ggcgcagatc agaaaaatac agcgcagaca agttctagta cgaaggaatc tgccgaagta
     4021 tcatcaggcg cttctgaaca agtatataca gatccaaaag aaatgaaaga atcttatgat
     4081 gtcgtgatta ttggagctgg tggtgcaggg atggcagcag ctatcgaagc aaaagatgct
     4141 gggttgaatc cagtaatttt ggaaaagatg cctgttgccg gaggaaacac atctaaatca
     4201 tcaagcggta tgaatgcttc agaaacaaaa tttcaaaaag aagaaggaat caaagactcg
     4261 aatgatgcct tctatgaaga aacgctaaaa ggtggtggag gaacgaatga taaagaaatg
     4321 cttcgatact ttgtcgatca ctctgctgat gcggtcgatt ggctagatca aaacggtatc
     4381 aaactggaca acttaacgat cactggaggg atgagtgaaa agaggacaca tcgtccgtct
     4441 gatggctcgg ctattggtgg atacttagtg gaggggttat tgaataacgt ccatgagcgg
     4501 gaaatcccta tttttgtcaa cgcagatgtg acagacatta agaaaaatgg ggaaacggtc
     4561 aatgaagtga ctgtacatgt tcaaggagaa gaaccaaaag aggtcaccgg aaaagcaatc
     4621 gtagttacca caggcggttt tggtgccagc aaagaattta ttgaaaaata tcgtcccgat
     4681 ttagctgatt atattacgac aaatcaagaa ggatcaactg gagatggtat caccatgatt
     4741 gaaaaattag gtggacaaac cgttgatatg gataaaatac aaattcatcc aactgtgcag
     4801 caggatgagg gggtcttgat tggtgaggtt gttcgcggag aaggagcaat cttggtcgat
     4861 caagagggga aaagatttgt caatgaaatg gatacaaggg ataaagtatc tgcagcaatt
     4921 acagcattgc ctgaaaaatc agcttatttg atttttgacc aaggcgtgcg tgatcgtgcc
     4981 acagctattg aattttatga ccaaaaagga tatgtcacaa ctggaaagac aatggaagaa
     5041 ttagcagaaa aaatcgattt accaaaagaa acactagcaa aaacagttga tggatggaat
     5101 acggatgttg cggagaaaaa agatgctcaa ttcaaccgtg ccacagccat ggaacatgac
     5161 ttatcgtctc cgaattatta tgcgatcaaa atcgcaccag gaatccatta cacgatgggc
     5221 ggtgtgaaaa tcaatacgaa cactgaagta ttagataaag aaaaccagcc aattaggggg
     5281 ttatatgctg ctggtgaagt cacaggtggc ttacatggaa acaaccggat cggtggaaat
     5341 tcagttgcgg agatcattat ttttgggcga caagcgggca tacaagcagc tcgatttgct
     5401 tcggaaaatg aatgaaatag aacgtaaata aaaagaggtt gtgaaatcag cgttttgagc
     5461 taataccgag ggtcagctga ttgaacactg tttagtgaag aggttgtgaa aaag
//
LOCUS       DACECZ010000138         5470 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.141, whole genome shotgun sequence.
ACCESSION   DACECZ010000138 DACECZ010000000
VERSION     DACECZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.141"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            33..1367
                     /locus_tag="IWO35_002447"
     CDS             33..1367
                     /locus_tag="IWO35_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317483.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            2003..2500
                     /gene="dfrG"
                     /locus_tag="IWO35_002448"
     CDS             2003..2500
                     /gene="dfrG"
                     /locus_tag="IWO35_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ0317484.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(2572..4524)
                     /locus_tag="IWO35_002449"
     CDS             complement(2572..4524)
                     /locus_tag="IWO35_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ0317485.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(4531..4827)
                     /locus_tag="IWO35_002450"
     CDS             complement(4531..4827)
                     /locus_tag="IWO35_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317486.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            5005..5470
                     /locus_tag="IWO35_002451"
                     /pseudo
     CDS             5005..5470
                     /locus_tag="IWO35_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1746 a          893 c          916 g         1915 t
ORIGIN      
        1 gtttttatta tggagaaaag ttttttaaaa agatagatgc tgctttttta tttagttgtt
       61 tatatacttt tgcaacttat cgaacagttg atatctatta tcgatccgca attgcagaag
      121 caatcgctct tacgattatc ccagctgttt tatattatgc ttatggattg atattcaata
      181 gagaaaaaaa agtcatcgct ttatctgtca gtataagtct acttgtgtat actcatgtct
      241 taactacact attgtgtagt gcattgattg cttgtatgat tatagttcgt ttgtttttaa
      301 gcaagcaaat aaaaaacgat ataaaatttg tggtcgtgaa tttagtaaag gctgtttttt
      361 tgacttgtgc attaactagc tatttttggt atcctatgtt gcagcagatg aattatcaag
      421 aaatacacag tcccttcaaa acagatttgc agctagaagc actttctatt tcagatagta
      481 tcattcaagc gttaaataac gatattacaa catacactat ggggttatta ggattggttg
      541 cgttgatttt tcctttattc atactgaaag aaatggagaa aaaagaaaaa atcatctatg
      601 ttggcgtagt ttctacatgg gtatgcgtaa ctaatttatt tccatggttt ttgtttcaaa
      661 aaacaccgat aagcttactc cagtttcctt ggcgtatctt aggtatccaa atcttttttt
      721 cttcaatgat cataacgctt gtttttatga aaaagactat gaataaaaaa tattcatggt
      781 taatcattgg cgctactgtg gtgtttctac tcgtcacgtc tatagcagca aaagaaaatt
      841 acgcaaaagt tatccaatca aaacacggtc atattgttat caatgaagaa acactcccac
      901 gttatacaac atcagatatg ggaggattat acgattacgc cccaacagaa gcgataaaag
      961 aaaggcctca cttagaaaaa catgaagtaa aagtaggaga atcgtgggaa aaagggaagt
     1021 ataaagctgc agataataac attacttaca cagtggcaag caacaaaaag caaaaagtga
     1081 cagtaccagt atatgcttat ttaggtacac aggtaaaagt aaacggtaaa gtcgtaaatg
     1141 actcttataa aaaacatggc ttagtgaatg tggatgtaaa tgctggagaa aataagatag
     1201 aaattacaac aaaatatact ccaactgctc tcatggcttt atggatcagt attctgactt
     1261 attgtttagt ggtgtattca agttttaaac gatttgcacc taataagttg aaagattctc
     1321 gatatgtgac taaaaaggag ataaacaaga agaatgaaaa taaataaaaa agtagtaata
     1381 ttgagtggat taattatagt tagtagtata tattctttca tctttttcaa aaatacacag
     1441 agtatttata ccactggaga tctttctttt cacttatctc gaatcaaggg gttatcgact
     1501 atttttacca gtccgattaa ttatgagaca ttcaattata ctggatatgg tgtaaattat
     1561 ttttatccct ttctgacttt ttttccagct gtaatgttat attggatgac aaaaaatttg
     1621 attgtaagct atattattta cgtttggtta ttgaacttgt gtacaacact cgtagcttat
     1681 cattatggcg agcggttttt aaaacaaaaa aaagcggcat ttttatttta agtagttcag
     1741 ttttggagta caaaatgaca aaatgtactt caaaatggac tacttttttc attttaactt
     1801 acataccaaa ttacatacaa aatgatttat aaaatgcaat tgaaaatgat aattgaatga
     1861 aaatttgtga ttttgtggta ttatgataat ataacttata gaaaaaattg cttattcaac
     1921 taacgggcag gataatgaga aaaggttttt tgatagaaat aagaaattag atggtaaatt
     1981 agttaaaaag gctgtgttat aaatgaaagt ttctttgatt gctgcgatgg ataagaatag
     2041 agtgattggc aaagagaatg acattccttg gaggattccc aaggactggg aatatgttaa
     2101 aaatactaca aagggacatc cgataatatt aggtaggaag aaccttgaat caatcggaag
     2161 agccttacct gacagaagaa atattattct gacgagagat aaggggttta cctttaatgg
     2221 ttgtgaaatt gttcattcaa tagaagatgt ttttgagtta tgtaaaaacg aagaagaaat
     2281 ttttattttc ggaggagaac agatttataa tttgtttttc ccttatgttg agaaaatgta
     2341 catcacaaaa atacatcatg aattcgaagg agatactttt tttccagaag tgaattatga
     2401 ggaatggaat gaggtatttg cccaaaaagg gataaagaat gataaaaatc cgtataacta
     2461 ctattttcat gtatatgaaa gaaaaaactt attgagttaa cgggtgcgtt aattgaagaa
     2521 gaacagtaaa gataaagcac aagagcttgg gattactcca agctcttttt gtcatttgaa
     2581 ataaattata tctttccaat caaattgttt atctgtaatc cctaatcttt gagcaggtgt
     2641 caaaaccttt ttatccgctg atttaaaagg gcgacaaaag ttgtagtaag ttcttagaat
     2701 tgtaatcgca aattgagcat acttcggatt gaagttagcg taaatatagc tcttcttatc
     2761 accacgagct gttgttaatg gtctttcaag agatgatata taacgtctta tctgttgaat
     2821 aaagctattg gtcgaatggt cattaacatt taatagcatc tttgcgattt cgtttggttc
     2881 cagagcagat aaatccgttc tacaatcaac tgaataaaat cctttatcct tagtggctaa
     2941 tggatgtttg atagggttat cggcatattt gcgatatctt tctccatctt tactaatagc
     3001 ctcttcgtgg aattgatgac ttgttagtaa ttcagttaga taatgagatg ccaaagtccg
     3061 tagaaacttt gttttgaatc ctctgatatc tccccaatcg agcagttctg cttttgcttg
     3121 tccgaattct tttaagcatt gttttctgct tttcgtcttg ttaacttggc aaataaaatg
     3181 atgtgcatcg ctcaacctta attctttcgt gaatactcta taaaaggcgt ttctaataga
     3241 gaagtcatca tcactaatca ttcgccattc gtcggcatta accatttgtt taattaacca
     3301 atagtgagca gttgtagtat aaggagcatc aacatttaaa ccatcgacaa atctactcct
     3361 attagtaatt ttccctaatt cgtgaagata agcattccta ttttcgctat cgttagcgga
     3421 tggttcttgt ggataataag accaatctaa cctatcattt ttacgactaa aagtattgag
     3481 atggtcttct ttgaattttc ttgtatcttc atttaattca tccattgaaa tattccaatc
     3541 ataggcaaca tcagaacgga acacatatct tgataagacc tcagcagata caactatata
     3601 cgtttgcata ttaaggtctt caaaccccgt atatttcttt gaaccttgtc ctttcttacg
     3661 gacgttattt aggtagtagt gcatcttgtc cgtattaagc cacatctcgt taaatttctt
     3721 tgtttgaagg ggttgtgtct catatcgttc taaaaactcc aaacaacgcc tatacagcca
     3781 ctccagctta tgatagtagg ttccaactcc aattcctaaa atatcacaag tacgactgac
     3841 tgaaaccctt ccaacaacca tcttagcgaa cattggaaga atggtgttct tttgttggtg
     3901 ataagtagtg gtttcttctc tttttggtag aacattagtg aacttcttac acgccttaca
     3961 ttgatagcgt tgagatttgc ccctactttt cccccttttg taaaattgct tcggctcatt
     4021 gaatggggtt gattcttctt ccgagcagcc ttctttatga aattgataat ctggctcaac
     4081 atcttggatg ctattgattt ctataaggcg tttaatttct tcaacaacag accaattaga
     4141 taaaggggtt actgtacagt tttgcgatac acctctgcca ataggattgt cattgcagta
     4201 cataccttta tgacctttat ctttggaact accagtcatt ttatatcgac ttggtttccc
     4261 ttttacatct ttgaatttgt gctgttcttt cccaaaatta caacaaaacg gattgatgca
     4321 gtaattatat tgaatgggat attcagaacc gttccattta aaagacgttg gcaagaacaa
     4381 cattttgtta taccttgttg caaatcgttt tggagttaat aaatcatatt gtttagccct
     4441 atcttttaat tcttcgtttg aaatcggaat tttaacatct acaatttcat cttccttcgt
     4501 ggctaatctt ttaagcttgg tcattgtcta tcaacccact ctatctttga tacccatttt
     4561 ttcaacaatc cgcttattac ttcgcttgtt ggcaatccat tcaataaact tcttatcgtc
     4621 taaaatgttc aataagaatt catccactgc ttcgctttca gtacgctctg catattcagc
     4681 gtattgctta actatcaccc ttacttgctc tgatatttcc caatccacag acttagcgtt
     4741 cttatttttt ggctcaagaa attgcatagg aaaatgcacc cctttttgct tagtaaattg
     4801 taaccatttt aacacaccaa tttgcattcc tacaataatt atttcacgtt cgcattttta
     4861 tatacatttt aacttacatt ttgtggtgta aattaacaac aaacgttgat ttaacaataa
     4921 aaaacgagta tcaaattcag ttgcaatttg atactcattt aggtttacat tttcactttt
     4981 tgtactccaa aactgaacta cttattttta tttagttgtc tctatatttt ctctgcttat
     5041 cggacagtag acatctacta tcgttccgca attgccgaag ctatcgcaat aacacttgtt
     5101 atccctgtat tattttatgc ttatcaaatc atctctggaa aagaagaaaa gtatccgtct
     5161 gtaaagctag cattgagtat gagtttgcta gtgtacagcc atgtactatc aacattaatg
     5221 agtacagcat taattatcat ttttattttt attcgtttgg tttctaaagg tttcaagaat
     5281 gcagatttta ttgctatatt taaaaaatta tttagtgcgg caggaatgac attggttctg
     5341 acgagtgctt tttggtatcc gatgtttgaa caaatgctct accaaaaaat caacaaacct
     5401 agcgtgacaa atttatatgc tcatgcatct aatgtgttcg atagtctgac tgaagctatg
     5461 aacaacgact
//
LOCUS       DACECZ010000139         5414 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.143, whole genome shotgun sequence.
ACCESSION   DACECZ010000139 DACECZ010000000
VERSION     DACECZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.143"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            94..2421
                     /locus_tag="IWO35_002452"
     CDS             94..2421
                     /locus_tag="IWO35_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0317487.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2547..2876
                     /locus_tag="IWO35_002453"
     CDS             2547..2876
                     /locus_tag="IWO35_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ0317488.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2916..3920
                     /locus_tag="IWO35_002454"
     CDS             2916..3920
                     /locus_tag="IWO35_002454"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0317489.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3917..5346
                     /locus_tag="IWO35_002455"
                     /pseudo
     CDS             3917..5346
                     /locus_tag="IWO35_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
BASE COUNT         1927 a         1035 c         1065 g         1387 t
ORIGIN      
        1 gccccaaact ttctatacat caataaaata ataaaaatta aatttagaat aacgtgtaaa
       61 ggattatttg atgatttttt attgagagga gggatgaaaa tgattaagaa aactcaaaat
      121 aaaatccaac gtatatgtca aaaacatttt gtttctagac tcaaagctgt atctaaaaga
      181 aaatacttac atcccattat gatttcgatc atcttgattt ttactttctt agggagctta
      241 tcaagtattt atgtaaacgt ttatacagaa acgtcaaatt ttgctaacca aacgacagag
      301 gagacaaata gtccacccta ttctgaaata cagtccatag aaagcaccaa tcagtctagc
      361 aacaacgaga caacctcaac cacatcctcg actcaactga ttgatccgac agaaaaagaa
      421 atcacagcag caaaagcagc aggggagaaa gcttattcag aaacaggtag aagccagaca
      481 tttagtactg ttgcggccac tgcgaatttc aacggacata ccataccaga tggatataca
      541 gtgatatatg ccaaagatgg agtcattgtt gcggttaacg gatcgatgag tgcggataaa
      601 actgcgttac aaggcgtaac caaagcaatc gcttttattg ataaccgtcc agatatccat
      661 aatctgttta ttggctccac agtaaatggt actactatca cattcccaaa aagtatctat
      721 atgggggtgg ctaccgctgg aaatacgatc gattcagtta cacccaatgc ggataaaagt
      781 tattctttta caccctcaac atggggaggg cttcctgccg gaggaggtat gagtactttt
      841 tcaatcggta tacagttagc aaatgatatt ccattcacgg ggtactcgat caaaaatggg
      901 cagatcgttg atggtaaggg aaatgtttca aattttccaa cctatcaagc agcaacggtt
      961 caggtcaatg cgaatcggac aggaactttt tcttatacag tcaatggtgg agcagcaaaa
     1021 atcggaacgt acgggactac attatctgga atccatgtgg gtgacaaagt ggttatcact
     1081 ccaacagcag taacgggcta ttcttttgca caaacaaacg gttctttcac agccctagaa
     1141 agtaacgtcg atacggtgac ctatacaggc aaacaaacac caatcactat taagtatcaa
     1201 gactattttg gacagatgat tgctccttct aaaataatga atcttactta cggaagtgct
     1261 gcacaagacc tgacgactaa tacgcctact atcaacggat atacctttac agctgtttca
     1321 gcgacagaaa caaagaacca atcagcaatg agtgttttag ctagtttaga tacaaatgga
     1381 aatgtaatcg taaaggacgc aaatggaaaa cagatttctg aagtcatcct atattataag
     1441 acaaatgcaa cagtatcaat caatgcaaat ggcagtaagt actatgatgg tctttctgtt
     1501 gtaccgatcg ttaaatatgc ctttaatgat aaaacagaat caacatccct tttaaacaat
     1561 gatctcagta ttgatcaaat cacatggaat ccaactgatt tcaaagcgat gaatgaaaag
     1621 ggagttgaag taactgcgcc tactgaaatt ggaacctata cgtcttggca attgacaaaa
     1681 agcggactag ctaaactagc tgccagaaca aactatttat ttactatcgt gcagacatct
     1741 gatgtctata cgattaagca aatcagcggt gcggtagagc tcagtggcag taagacatat
     1801 gatggaaaag caggtgtccc gtcaatccat gtcaagctag cagagggtgt cactagttcg
     1861 cttactccag tacaagtggc acttagcagt atggattaca cagcagatgc acaatcagca
     1921 aaaaatatgg ttaatgctgg cagctataca attaaactaa cgaatagcgg aatagacaaa
     1981 gtcaaagcag caaatcccaa gttgtcattt actgatatag ccaacacatc aggaacttat
     2041 accatctata aagctaacgc agtgattact gtcgatgacg ctgcctttaa ttatgatgcc
     2101 caaagtcact cgatttcggt tggaaatctc cacgttaccg gtgttgtctt aggcgaatca
     2161 ttggattata ccctaacagt taacagccgg acagatattg gatcacaaat agtaacgatt
     2221 agtttaggcg aaggaagtgt aaacaataat tatacgatta tcgcaacaga taccgctaag
     2281 ttgacgatca atccgactct cttgcttcct tcgacaggtg ggataggtct actgcccttt
     2341 gtctttttag gactgatctt tattttttct ggcttctttt attttatcca tcgtaagaaa
     2401 aaagcaggtg aacagagatg aaaaaaacaa aaaatgtacg tgtggatata tttgctgttc
     2461 tcttttttat cctactggtc ttttttatag gttctccgac tgtgtcagct agagaatcac
     2521 ctattacatt tactttgcat aatcgaatgt tcgaaagcga taagatgcca agtatcagtc
     2581 aaaacgacgg aataaatatc ccggataact ttcaaggagt aaaagggttg aacggcgtta
     2641 ctttcgctat atatgatgtc tcggatgaat tttacaagct gcggtcagaa ggaagtagtg
     2701 tggaagacgc acaaagaaaa ctagcacaaa aaagtgatag tgaaaaaata ctggcagaaa
     2761 atgtgacaaa aacagtagac gaggaagagg gcattgcttc ttttagtgca tcggacaaag
     2821 atgagcaagg tcgtgacgct gtttaccgat ttgcggaaat aaagacttca gattaagtaa
     2881 aagaaaaatc agcaccattt gtcgttgtgc ttcctgtgac agattcatct aatcataaat
     2941 tgacgaccat ccacttgtat ccaaagagcg aacaaaaaat acctgctgca ctcacattaa
     3001 caaaaacagt agagaataag cagacagatt ttgcagatgg ggataaagtt ccttatctca
     3061 tcaccgtaac aattcctgag aacatcaacg agataggaac ctatacgatc aaagatacag
     3121 ctgatcctca gttatggcta gaaccagaaa cgatcgattt tttgattggt ggagacaaga
     3181 ttcatacatt tcacacacaa aaaacaaaac atggttttgt cttatcagat tctggaaaag
     3241 atcttagttc gtttgctggg aaaaaattaa cgatccgtta ccaaatgaca ttgaaagaaa
     3301 acagtgaaaa aacgacattt gataacgatg tacgtttgac gactgacaat caaacagtag
     3361 aaacacatct tgccatccaa acaggaggca aacaatttgt caaagtggat cggcaaacga
     3421 aacaaaaact agcagatgct caatttctag tcaaaaaaaa tgaccaatac ttagcgcaag
     3481 aaaacgggat aaatcattgg gtagccaaag atgattcaag agcaacgatt tttacttcca
     3541 gtaaagatgg aacattttct gtccatggat tgtcttttgg aagttacgaa ctagtggaga
     3601 ttaaaccgcc taaaggatat atattgagtc aatcaaccat tctctttgaa gttgaaaaag
     3661 gcagttatga tccgcttcat cctataccat tggaaatcat caacgaacca aaagcaaatc
     3721 cgacgaataa tggaaaaacg ccagttcaga tgcagacaaa tggtccatca aatgaaaaaa
     3781 atggaggaag ctttccaaaa acaagcgaag agatgcttgg aggtttctca attttagggt
     3841 tgttgctagt cctttcaaca gggacagctt ggttttataa aaaacaacaa aaaggaaaca
     3901 ataggaggga aataaaatga aaaacatcaa aacaaaaata tggacgtttt taggaactgc
     3961 tatcatgctg cttcctttcg ttctcggcct aggaactgca gaggtttcta ggccgtatca
     4021 cctacaccag aaaatgttac ggttaatttg cataaattga aattcacttc agcaccagaa
     4081 aatcaaataa acaacggaac ggaattgaca tttccgaata gcgaaccgtt gaacggcgtt
     4141 gaattcaacg tatatgacat cactgctacg tactatccaa gtaaagacac agcagttcca
     4201 gcagacgcta cacccttcgc aagtgtaacg acttctggtg aaggattagc taacctgact
     4261 cttccaggta aatctgatgg gaaagatgct gtatacgtct ttgtagaaac accaaagccc
     4321 ggtgtcgaaa cgtcgccaaa tatcgtgtta agcttaccct tttataaccc gaattcatca
     4381 gaccctgaca aacctttaga cacgattcac ctatatgcta aaaatgtcat caaagaaagc
     4441 aatatcaaaa tccagaaagc tgggaacttt tcaggggtag ataagttagc aggggcgcaa
     4501 ttccagatca aaaattcaga gaatcaattc gtgaccggtt ttgatgaaaa cactggtgtc
     4561 gctctctatg atcctgctga gtcagcgttg acattcacaa ctgatactga aggatccatc
     4621 agtgtcaatg gattaataga cggcaattat actttagttg aaacaaaagc accagatggt
     4681 tatcaattaa ccaatccaca aaaagaaact acttttatag taaaaaacgg aaaattgcaa
     4741 caaacaaccg gtatcatcca tggcgcagat gaagccaatc ttgggaatcc aaacggtgta
     4801 gtcaatcggg aaaatacaac tacggataac acgattactg tagaaaatgt ccaaaactac
     4861 ggtgatttcc acttcatcaa acaagatgtg aacacgcaag aaaaattagc cgatgcagaa
     4921 ttttctattc taagagcaaa agatccaact gttaaattaa tgaaaaaaat agcaaatctt
     4981 gcctcaggag aatacccata tgcttggtca gatgaagtca caggttctga ctgggaagaa
     5041 gtgaaattga tttctgattc agaagggaaa ttttcaatcg aagggctgaa acaaggggca
     5101 tactgccttc aagaaacaaa agcaccagaa ggctacgcat tacctaagaa tgaagctgcg
     5161 tacactgaat ttacagtaga tgatgatcca acaacttctg atgaaacaac gatcgataac
     5221 caacctaaag gaatcttgcc ttcaactggt ggtatgggga tcatcgtatt catcttggta
     5281 ggtactgctt tagttggagg agcagtgatc tacttcaaaa aacgccatca agaaacagaa
     5341 gcttaaacta ttggaaacaa aaatcatacg ataaataaac gatagtgatt caaatgggga
     5401 gaaaggttgt gaca
//
LOCUS       DACECZ010000140         5387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.144, whole genome shotgun sequence.
ACCESSION   DACECZ010000140 DACECZ010000000
VERSION     DACECZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.144"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(105..563)
                     /locus_tag="IWO35_002456"
     CDS             complement(105..563)
                     /locus_tag="IWO35_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0317490.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(576..1901)
                     /locus_tag="IWO35_002457"
     CDS             complement(576..1901)
                     /locus_tag="IWO35_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ0317491.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1918..3330)
                     /locus_tag="IWO35_002458"
     CDS             complement(1918..3330)
                     /locus_tag="IWO35_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0317492.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3347..4111)
                     /locus_tag="IWO35_002459"
     CDS             complement(3347..4111)
                     /locus_tag="IWO35_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ0317493.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4215..5090)
                     /locus_tag="IWO35_002460"
     CDS             complement(4215..5090)
                     /locus_tag="IWO35_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0317494.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         1650 a         1131 c          787 g         1819 t
ORIGIN      
        1 caaaaatcaa aatgattaaa tctagtgatt gaaacatatc atttcaaaaa aattttgtaa
       61 attcacccat aaatatcacc ctatttaact tttttcatcg tacactaaaa ttcaaaacaa
      121 ctatctttaa tctttacttc ttggttttgt tttaataaat ttccttcaaa aatgcctgga
      181 gaaactacaa tgatcaccgt atctttttgt gcttgctcca acttcttcaa atctataata
      241 gctaacagtg tcccttcttt tacctcctgg ttactttcaa cccgtatctg aaacggactt
      301 ccttcgagtt caactgtatc taaacccata tggatcagta tttttctgcc agattcactt
      361 tcgatcgtta tggcatgtcg ggtaggaaat atattatcga ttattccttt aactggcgca
      421 tggatatatc catcatgatt cgttatagcg tatccaactc ctatcatttt tgaagaaaag
      481 acctcgtctt ttactgctgt taatggaata atttgaccac tagctacagc ttttagaatg
      541 tttcttgttt taaagaaatg cactttttat cacctctact ttaatgctgg ccaatatttt
      601 ttgtttactt tgatcatttc atctagtatt ttttttgcga ctctggcatt tggtacggtt
      661 tgattcaatg taaatgcttg taaagctttt tgataagaac cttcaaagta agcatctact
      721 agtaacttct ctactgcaac ttgtgcctcc atcaatcctt tatgaaaatc actgattggt
      781 tttcttagtg taattggctc cacaccagtt gcaccaacat atgccggtat ttcaacaact
      841 gcatctgacc gtagattcgg aatcgcccct tgattaggaa cgattagcat aaagcgacga
      901 cgatcatcat tcaacaagga aatagctata tcgacgatat attgtccatg ctccccaaaa
      961 ttaaagttca atacttcttc cacaataccg cggcgtattt tatcagccat ttctttggta
     1021 tttttctctc gtccatccat gaccatatta gcacgtgtat actctttgtc agtatgctca
     1081 acgaccatat caggatataa gtaatactct aagtaattat tcgggatatt acttggaaaa
     1141 tttttcgtga tgactgacat catttgaaag gttttttgcc aagttttatc tcctatatta
     1201 aaatcggcga cttgcatttc ttttgttatt agtttttcaa taatctctgg cataatttct
     1261 cgttgcaatt ctttatcata aatcgatgta taccagccaa agtggtttaa accataatac
     1321 gtaacgatcc aattcttacg atcataaccg taattcactg caatcgtttc ttcaattgaa
     1381 atagtcatat cacaggcatt aatcattttt acattcggat atttacgacg aacagcttca
     1441 gaaacgatcg actctggatt tgtataattc aaaatccatg ctttgggggc atagtcttgt
     1501 atatgatcga caatttccaa taaacctttg attgagcgca tcccataagc aaatcctccg
     1561 agtccacacg tttcttgtcc tactaatcca tgtttaagtg gaatcttttc gtccttttct
     1621 cgcatctcca atcctccaac acgaatttgt gagaaaacaa aatcgcatcc tgtaaaagca
     1681 agctttggat catccgttgc acgaatgcga atatttgaaa aaccttttct tttcagcata
     1741 aatttaacaa tcaagtacat atccatattt ctttcttcat caatatcata taaacgtatt
     1801 tcagataatg gcaaccgttt ttcattgttc aaaatggctt gaacgattcc tggtgtatac
     1861 gtactcccgc ctccagctat agtgataatc tgttttttca tcatcatctt ttcctttcta
     1921 ttcttctaat aatttattga attctgccat aacataagtc acttctaagc cgatgataat
     1981 ttgataattt ttttcagttg gtttaatgat cccgcttatc cctaattgct tgattcgctg
     2041 ttcctcgacc atggtgggat ccactacctc tacccgtagg cgtgtattac aactatagac
     2101 atccaggata ttggcttttc ctccaaacgc ttgtagcatt tttgttgcca tatattcata
     2161 atttttactt gctaacccta actctgtttc ttctaaggtt acttcattgc caaactctgc
     2221 cttagcttca cgtcctaatg tctgaatgtt tctcttattg atcaaataat aaaaagtaac
     2281 gtaatacaag gcaaaaaaga aaattccgat tggaagaatc aaaatagcat tggtagcgat
     2341 tcgaaaattg atgagataat caacaataca tgctccgaat gtaaaaccaa ttcgtatatt
     2401 gaataagtaa cagacaatcc ctgctaaacc ggcaaaaagt gcatggatca cgtacaacat
     2461 aggagaaaga aacatgaaac taaattcaac aggctcagtg atatttgcaa taaatgaagt
     2521 tcctgcaccg cttcccatga ttcctttgac catctcttta ttttctttat aagctgcttt
     2581 atagatagct agacatatag ctggtatacc aaacatcatt actacaaaaa aaactgataa
     2641 aaacagtcca gcctgaggat ctccgttgat aaagcgtgtc atctctcctc tgaaaacttc
     2701 cttgctagga gatgtatagc ttcctaaatc gtaataaata tacgtattca acacatggtg
     2761 cagtccaaag gggatcagca aacgattcaa aaacataaat atccccacac ctaatgctcc
     2821 catatcaacg agcagttttg caaaattatc aatcccaatt tggatgagcg gccagataaa
     2881 gcccaaaaaa acagctgcga ttgtttgtag aatcatcacc atagtcaatg ggaatttttc
     2941 gcccgcaaaa taagaaaaaa ctactggtaa tttttgttct ttaaagcgat tatacgtcca
     3001 tgccgctact aaaccggaaa taattccacc aaaaatcccc atattcactt ctgggtccat
     3061 aatttctaag ccttttttca aggtagcaat cgctaaaaaa ccagtaagtg caggtattcc
     3121 tttgtcctta tttttgacaa aaccgattgc aactcctata gcaaataaga tatccaagtt
     3181 tccaaaaacg gcatttccag ctgcaccaat tactgggatg ttcagcatat cgtcagctga
     3241 taaacgcatg atcaatcccg ctaatggcat cgctgcaact ggaagtagca tcgctcttcc
     3301 aagcttctgc acatttgatt taacattcat ttttattcct cctctattaa atatcctcat
     3361 atgttactaa ctcaatcaaa ggatcatttt tcactttttg agcaaatgct gaatgaatta
     3421 aaaattcgac ttggtgggta cgaggataca caaaacttga accatttaac aattcctgat
     3481 ccaaatacgc tgcgtgacac atgacttcga tcgttttatc ttctctcaat atcatgagta
     3541 aacttgccaa atattcttcc acactcgtat tgatttgtcg ttttttttca tcagtttctc
     3601 ctacgacatc gaaacaaggc tcaaaataat cgacatgacg aacttcatct tttcgttcaa
     3661 agttgctccg aaccggcaaa tcatattttt ttgctaatgt aacaactacc tcctgatttt
     3721 catccaaagt atgagtatga tgatgactgt ctaaatgagt tggtatgatc cccgcatgat
     3781 aaattttttg aatctgagca ttccattcct gatacagttg atctttgtca atgttaaatg
     3841 gatgctgata ataagataag gatctgaact ctccgctttc gataagagta tcgacagttt
     3901 tgaggagtgg tttgccacac gtcaatgtta aatggactcc tacacctagc ctaggcattt
     3961 cctttcttag tttcacagca tgctcgaaac ccggcatatt tgccatcaat gtagttgatg
     4021 tcaaaattcc tcgctgataa gcatccataa tcccataatt cacaccatgg ctatagccaa
     4081 aatcatccga attaaaaatc acttttccca tttcattctc cttctttttt ctactaaaat
     4141 aggcgcaacc aaataatcat agtccattta ctatgtgtca tttggttgcg cctatagata
     4201 gttacgccct tattttaata gattcttgct agatgaattg ccaaatagat gatttcattt
     4261 tcttgaatgg caagttgaaa ttgctgttcg atgatatctt taatctttag tgctgtacaa
     4321 tacgctttgc tatgtttaaa tgtttcagta aaaaaaccaa tattttctac tggcgtttct
     4381 tcctgataaa tcaagcgttt aaccaggaat ttgatatgaa ccattaagcg atgataagtc
     4441 agtgattctt tatcataaac gtttgaatag gatttttcta gatgggacaa aatcgtggtc
     4501 gtcatttcta ctatttttaa gctctttaaa ctattaggct ctcctagctc agcattaatg
     4561 atatgcatag tgatgaatcc tacttcattt gaccctaact cggtttgata tcgatgattg
     4621 attcgctcga ccacttctac tgctatatca tattcatttg gataaaggaa tctcatttct
     4681 ggcaacataa ttgctgtgac atctctatct tctctttttc gttgaatagc aaaataaata
     4741 tgatctgtca atgtaatata gatatttgaa tctaatggag aggagatcct agctgttatc
     4801 agttcaatca cttcttccgc aaaatccata aattcgattg gaatctgcga gactatctca
     4861 ctgaatcgtt tgatcttttc tttagaatct aatacaaata atttgtcagc atcctcaaca
     4921 tcaaatggct tactcacttt agcagaaaag ccaatccctt tccccatcgc aattttttct
     4981 attccatcgt catcgatcaa tacagcatta tgattcagct ttttcatcac atacaccgtt
     5041 aattcacctc ctctatatga taaaacaaaa caacttgcag actccttcat acatattttt
     5101 gtctccaaac aatttttaaa gacaaaaaac gacctaagaa agaacccttt cttaggtcgt
     5161 gcctaattga atagtagcac cctacataat ttacaaaaat agtttaacac ataataaaag
     5221 cgttatcaat atctttttta ctcgttttat gataatactt gtatcgaatt gatttggagg
     5281 taattacata aaaacaaaaa aaatatagca actctctata agaaagttgc tatattgata
     5341 attataccgg cggccggggt cgaaccggca cgtcctcaac ggacact
//
LOCUS       DACECZ010000141         5341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.145, whole genome shotgun sequence.
ACCESSION   DACECZ010000141 DACECZ010000000
VERSION     DACECZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.145"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            401..1312
                     /locus_tag="IWO35_002461"
     CDS             401..1312
                     /locus_tag="IWO35_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0317495.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            1766..2080
                     /locus_tag="IWO35_002462"
     CDS             1766..2080
                     /locus_tag="IWO35_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0317496.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            2096..2482
                     /locus_tag="IWO35_002463"
     CDS             2096..2482
                     /locus_tag="IWO35_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0317497.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            2511..3896
                     /locus_tag="IWO35_002464"
     CDS             2511..3896
                     /locus_tag="IWO35_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0317498.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            3899..4051
                     /locus_tag="IWO35_002465"
     CDS             3899..4051
                     /locus_tag="IWO35_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0317499.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            4074..5279
                     /locus_tag="IWO35_002466"
     CDS             4074..5279
                     /locus_tag="IWO35_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0317500.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT         1862 a          922 c         1101 g         1456 t
ORIGIN      
        1 tatttttggt agttttaata tattcatttt tagttgtata aaaatatatc ataataaaaa
       61 caacaaaaaa atgtctggaa aatcgaacat aaaagtgtct gatttgaata tcccagcagc
      121 ggaagaagaa aaacaaaatc gataacattt gttctaagaa ttagttacgt taaatacgga
      181 acgcaagata gacttgaaaa acggtaacaa aagaagtagt atattaaggt gtgatgtgct
      241 acttgacatt tattctgctg agcgaacttt ctggattgtg tagccttata tctattcagg
      301 acgtatcgag cagattagta aaggtgttct tgatgatttt catcttgaac acctttgttt
      361 ttagcaattc gcaaaaaacg aaaagtagga gaattgataa atgaaaaaaa gatttttagc
      421 acttgccatc gtactaggca ctggcttatt gtcaggctgt acgaatgccg gtgaaaaaac
      481 agccgttagt tataaaggcg gtacaatcag cgaacaagaa gtaatggata gcttgaaaaa
      541 aatgcaagga gcagattccg ctgtgcaaca gttgatcgtt tatcaagtat ttgaagataa
      601 atatggagat gacgtatcaa ccaaagaaat cgattctcaa tatgatcaaa caaagaaaca
      661 attgggtgat tcatttgatt cacaattgaa atctgcaggt tacacagaac aaacattcaa
      721 agacagtatc aaacaaagtc tagcctttca agaaggtcta aagaaacata tcaaattgac
      781 cgatgaagat ttaaaaacgg cttgggaatc tttccatcca gaagtggaag ctcaaattat
      841 ccaagtagca agtgaagatg atgcaaaaga tgtgaagaaa gctgctgata agggagacga
      901 tttctctaaa ttagccaaag acaagtcaac tgatactacg acaaaagaag atggcggaaa
      961 agtgaaattt gattccacta ctacaacggt tccggcagaa gtcaaagaag cagcctttaa
     1021 gttgaaagat ggtcaagttt ctgatgtgat cacttctacc aatgcttcaa cttatactac
     1081 ggaatattat gtggttaaaa tggtgaaaaa ccaaaacaaa ggcaacgaca tggataaata
     1141 caaaaaagaa ttgaaagaaa ttgcaacaga cacaaaatta agtgacagta ccttccagaa
     1201 taaagtaatt ggcgaagtat tgaaagacgc taatgtaaaa atcaaagaca aagactttga
     1261 aaatgttttg agtacattca cttcagattc tagtacagca agtagtaaat aagcggtata
     1321 aataaaaaat atgaactatt taaatcaata aaactgttca tctaaatctt tttattagaa
     1381 ctaaatcaac agtagtttaa ttactacttg atttagttct ttttctacaa aaaatagcat
     1441 aaaaatctag ttatccgcat aaaaactgga cttatcacac tttatcaagg tcaaaaccac
     1501 tcaatttact actaatttac tacttatgaa tgagctttga tacgacgatt tatccttgaa
     1561 aagtgaagat ataaagatac ttccaataaa atttgaatat ttaataggta gacacttcaa
     1621 aaaatgaggt gtctattttt ttacccgatt ttgaaaggaa gtgaacttat gaaaacaaaa
     1681 aatcaagaat caaaaggtcg ttccccactc tttaagacca tcaaacattc attcagccaa
     1741 taaaaaagaa aggataggta aaaatatgga acttaaattt gtgattccca acatggaaaa
     1801 aacattcggc aatttagaat ttgctggcga ggataaagtc gttcagcgaa gaatcaacgg
     1861 acggctaact gtcttatcaa gaagctataa tctctattct gatgttcaaa gagcagatga
     1921 tattgtggtg gtgcttcctg ctgaagctgg cgaaaaacat ttcggctttg aggaacgtgt
     1981 gaagttagtc aatccacgta ttaccgcaga gggctacaaa atcggcactc gtggttttac
     2041 aaattacctt ttacatgctg acgacatgat aaaagaataa agaaagagag gaaaaatgat
     2101 gagattagca aatggcattg tattagataa agacacgact tttggagaat tgaaattctc
     2161 tgctctacgt cgtgaagtga gaatccaaaa tgaagacggg tcggtttcag atgaaatcaa
     2221 ggaacgtacc tatgacttaa aatccaaagg acaaggacgc atgattcaag taagtattcc
     2281 tgccagcgtg cctttgaaag agtttgatta taacgcacgg gtggaactta tcaatcccat
     2341 tgcggacacc gttgctactg ccacctatca aggagcagat gttgactggt atatcaaggc
     2401 agacgatatt gtgctgacaa aggattctag ttcattcaaa gctcaaccac aagcaaagaa
     2461 agaaccgaca caagacaaat agtcgctagg tagaaaggag actttttcgc atgaaacagc
     2521 gtggtaaaag gattcgccca tctggtaaag atttagtctt tcattttacg atagcgtcac
     2581 tcctgcctgt tttcctgctg gttgtcggac tgtttcatgt gaagacaatc cagcagatca
     2641 actggcagga ttttaaccta tcacaagcag ataagattga cattccctat ttaattatca
     2701 gtttcagtgt cgcaattctt atctgcttgc tggtagcgtt tgtattcaaa cgggttcgct
     2761 atgatacggt taaacaactt taccaccgtc aaaaactggc aaagatgata cttgaaaaca
     2821 agtggtatga atctgaacag gtcaaaacag agggtttctt taaagatagt gctggtcgta
     2881 caaaggaaaa gataacctac ttccctaaaa tgtattatcg acttaaaaat ggcttgatac
     2941 agatacgggt ggaaatcacg ctgggaaaat atcaagacca actcttacac ttggaaaaga
     3001 aattagagag tggcttgtac tgtgagctga cggataaaga gttaaaggat tcctatgtgg
     3061 aatatacttt gctctatgac accatagcca gtcgtatttc tattgatgaa gtagaagcta
     3121 aagatggtaa acttcgctta atgaaaaacg tatggtggga atatgataag ctccctcata
     3181 tgttgattgc tggtggtaca ggtggcggta aaacttactt tatactgaca ctgattgaag
     3241 ccttgcttca tacagattca aaactgtata ttcttgaccc gaaaaatgct gaccttgcgg
     3301 acttaggttc tgtgatggca aatgtctact atagaaaaga agacttgctt tcttgcattg
     3361 aaacattcta tgaagaaatg atgaaacgta gtgaggaaat gaagcagatg aagaactata
     3421 agactggcaa aaattatgct tacttaggtc tcccggcaca cttcttaatc tttgatgaat
     3481 acgtcgcttt catggaaatg ctgggaacaa aagaaaacac cgcagttatg aataagctga
     3541 aacagattgt catgttaggt cgtcaagctg gcttctttct aatactggct tgtcaacgtc
     3601 cagacgcaaa atatttaggc gacggaatcc gtgatcagtt taatttcaga gtggctttag
     3661 gtcgtatgtc tgaaatgggc tatggcatga tgtttggcag tgacgtacaa aaggatttct
     3721 tcttaaagcg aatcaaaggt cgtggctatg ttgatgtagg aacaagtgtc atatcagagt
     3781 tttatactcc ccttgtacca aaaggatatg atttcttgga ggaaattaaa aagttatcca
     3841 acagcagaca gtccacgcag gcgacgtgcg aagcggaagt cgcaggtgtg gactgatctt
     3901 gctggctggt gtggcaatag ccacgccagc acttaacccc ccgtatctaa caggggggta
     3961 caaatcgaca ggaaacagtc aaaaaaacat tagaaaatcc tttggttaca agggatttac
     4021 aaaatttcag cgtatgtcaa atgggcttta aaagttgaca tacgcctttt tgattggagg
     4081 gatttttact gaatgaacaa acttggttac agcatttaaa agaaaaacgc ttggcttatg
     4141 gactatctca aaaccgttta gctgttgcga ctggtattac aaggcagtat ctaagcgata
     4201 ttgaaacagg aaaagtcaag ccatcagagg atttacagca gtccctttgg gaagctctgg
     4261 aacgcttcaa tcccgacgct ccccttgaaa tgctgtttga ttatgtaagg attcgctttc
     4321 cgacaacaga cgtacagcag gtggtcgaaa acatcttaca actgaaactg tcctattttc
     4381 ttcatgagga ctatggtttc tattcttatt cagagcatta tgctttaggc gacatattcg
     4441 tcctttgctc ccatgaactg gacaaaggag ttctggtgga attgaaaggt cgtgggtgca
     4501 gacaatttga aagctatctt ctggcacaac aaagaagctg gtatgagttc tttatggacg
     4561 ttttggtggc tggcggtgtg atgaaacgcc ttgaccttgc cattaacgat aagacaggga
     4621 ttttgaatat ccctgtactc actgaaaagt gccaacagga agaatgtatc tccgtcttcc
     4681 gcagttttaa aagctatcgc agtggcgaac tggtacgcaa agaggaaaag gaatgtatgg
     4741 gaaacaccct ctatatcggt tcattacaaa gtgaagttta tttctgtatc tatgaaaagg
     4801 actacgagca gtacaagaaa aatgatattc ccattgaaga cgcagaagta aaaaaccgtt
     4861 ttgagattcg attgaaaaat gagcgtgcct attatgcagt ccgtgattta ctcgtctatg
     4921 acaatccaga gcataccgcc tttaaaatta tcaatcggta tatccgtttt gtagataaag
     4981 acgattccaa acctcgttct gattggaaac tgaatgaaga atgggcttgg tttattggga
     5041 acaatcgtga acgattaaaa ctaaccacaa aaccagagcc ttactccttc caaaggacgc
     5101 tgaactggct atctcatcaa gttgccccga ccttaaaggt tgcgattaaa cttgatgaaa
     5161 tcaaccagac gcaggttgta aaagacattc tcgaccatgc gaaactgaca gaccgacaca
     5221 agcagatttt gaagcaacag tcagtaaaag aacaggacgt gataacaaca aaaaaataac
     5281 tcaaatacaa attcattgaa tatagagagg agaacatttt tatgaatttt ggacaaaacc
     5341 t
//
LOCUS       DACECZ010000142         5286 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.146, whole genome shotgun sequence.
ACCESSION   DACECZ010000142 DACECZ010000000
VERSION     DACECZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.146"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(44..410)
                     /gene="ssrA"
                     /locus_tag="IWO35_002467"
     tmRNA           complement(44..410)
                     /gene="ssrA"
                     /locus_tag="IWO35_002467"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(581..1354)
                     /locus_tag="IWO35_002468"
     CDS             complement(581..1354)
                     /locus_tag="IWO35_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0317501.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1498..1848
                     /locus_tag="IWO35_002469"
     CDS             1498..1848
                     /locus_tag="IWO35_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ0317502.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1829..2545
                     /locus_tag="IWO35_002470"
     CDS             1829..2545
                     /locus_tag="IWO35_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0317503.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2545..3993
                     /locus_tag="IWO35_002471"
     CDS             2545..3993
                     /locus_tag="IWO35_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0317504.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4063..4572)
                     /locus_tag="IWO35_002472"
     CDS             complement(4063..4572)
                     /locus_tag="IWO35_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317505.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4562..>5286)
                     /locus_tag="IWO35_002473"
     CDS             complement(4562..>5286)
                     /locus_tag="IWO35_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317506.1"
                     /translation="KIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSAL
                     ANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNKQ
                     ELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVLK
                     TTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLIL
                     SLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1787 a          928 c         1062 g         1509 t
ORIGIN      
        1 aaaataagga tttaagcatc tagcgaaatc tgctgaaatc tcacaatgga gacggcggga
       61 gtcgaacccg cgtccaaaca catcgccact taaaaatcta cgttcatagt caactcattt
      121 aaggattcac tttacagctt gccgagtgac aggcattctg tatcgctagt ctgatgttct
      181 ctttttcaac ttacagacgg aaagttgaaa cgtatcccac taaagttagg acccttactc
      241 gagcacatgg gcgatgccga gaggatctac gctaactgtt tttaggcagc taaagcgtaa
      301 gagttgtttt cgtttttagc agttatattt aactgtaacg tttttacgta gacgtaacct
      361 acgaaacgca attcaagctc gactgtgcct gtcgaatccg taacgtcccc gttagctaac
      421 aagggttacg tgtatttttc atacaaaatt agtatagcat atattgggat aaattcaata
      481 aaaaagcgaa tcatatccca ttatttatat aataactgcc cctttcttaa gaaaaaataa
      541 gcgattttct taagaaaaga ggtagcttca aaaaaagatc ttaaatcaga tcaaaatact
      601 tcaatgcgtg gacaattccg ccttctgtat ttttctttgt gataaaggta gaaagttctt
      661 ttaatccgtc ataggcgttg cccatagcga ttttgttgtc gcaagcttcc aacaaggcaa
      721 aatcattcgg accatcgcca aatccaaaag taggaacttt ttccagctga agttcttttt
      781 gcagacgttt gacacctgcc ccttttgaga ttcctttctt cactgtatcg atcgagtaag
      841 gaccattccg ataaaaagtt agctcaggaa aatgttcgtg atagtattcg tcacctgatt
      901 gagataagat cagcaacata tttactgtgt tttctttaaa gcctaaagga tcaatttccg
      961 gaacatctga atgaatgaag tcgtaagcat tgatcattgt ttggctatga ccggtacacc
     1021 agattttctg atcgttatag taagaaagtt catgaccgtt caactgtaca tgatcataca
     1081 ttttttgaca ttcttctgaa gaaaatgttt gagaatagat ttcttcgccg tctactcgaa
     1141 tgaaagaacc attcatggtg accgcagaag taatgcccgc atcttccatg atagagtgga
     1201 tttctgcttc tgtacgtcct gtagcgatga cggggagaac attgttttct tttaatgcgt
     1261 tcatagcttt tgccacttcg ggtgtgattt ttgatttcgc atctagtaat gttccatcaa
     1321 gatcaaagaa agtgattgca cgataatttg acacaaataa aactcctttt ttattggaat
     1381 aagtttatct taacaaagaa tgaacaaaag acaaaccttt cgtgaagaat tcctgaacat
     1441 attggttcag tcaacaattt agagtatact ataagaagag aatttaggag ggaaagaatg
     1501 atttttttca gtattggtat cttattctta ataattggca gtctttttcg gattctgccc
     1561 tcaaaaggaa atctgccttt ttatgggtat cattcgcctc ttgccgcaaa aacagacgct
     1621 cattggcggc tggctcaaaa gacaagcgga aactggtttt ttcttatggg cttactgatg
     1681 gcattgatcg gctattattt aaagacatct gggcatacga attattttct gattgaaatg
     1741 cttctgctag tatttccaat tatgcctatt tttattatga cagaaaagaa attacagaaa
     1801 tatgacctgg aaactggagg gaacgataat gaatatttta atgattgaag ataatcaatc
     1861 tgtttctgaa atgatgcaga tgtttttctt aaatgaaggt tgggaagcaa ctttcaagta
     1921 tgatggaaaa gaagggctcg atgccttttt agaaaaccca caacattggg atatgattac
     1981 actagacctg aatttgccca cactggacgg tatgctagta ggaagagaga tccgcaaagt
     2041 ttcaagtact gtgccgatta tcatgctgac agctagagat tctgaaagcg atcaggtgat
     2101 cggcttagag atgggggcag atgattatgt cacaaaacct ttcagtcctc tgacgcttat
     2161 tgctcgaatg aaagctttgc atcgccgtag tgagttggca gaagcaagag aagaagcagt
     2221 agtttcagat gatcattttg atatcgagac tagtcatttc aagatgaata cgaaaacacg
     2281 agaagcgtat ctggacaacc ggccaattga aggactgacg cctaaagagt tcgatctatt
     2341 gtttacttta gcaaaaaaac cacgtcaagt attctctcgg gagcagttgc ttgaattagt
     2401 ctgggattat cagtactttg gtgatgaacg gacagtggac gcccatatca agaaattacg
     2461 acaaaaaatc gaaaaggtcg gaccacaagt catccaaact gtttggggag tcggctacaa
     2521 attcgatgat tctggtgttg cctaatgcgt tatttgtatc agcaactctt agctttctgc
     2581 ggaatggtcg cgttgatctt attgatcgtt ggagtttctt ttacacaact gacaaaacaa
     2641 acgattgaag aaaacaatta ccagcaactg tttggatatg ctgaatcagt tgaaaagaca
     2701 acgcaaacct atgccgatac gctcccgcag tttagtcaag atcaggcgtt tcaaaatgca
     2761 ttgctgctga cagagcaagt actgacagaa caaaatgtga actttatatt tatcgataaa
     2821 aacgaacgtg tcatttatcc gactaataca gctttactga attttacctt aacagctgat
     2881 caatggaaaa atctgaggaa tggacgtcaa gaaaaattca catcgaataa aaatatttta
     2941 ggacaagcac aggcgacctc ttacgcactt gtgccattta atttgaatca tgaattctat
     3001 ggagcacttg tagttagtca acctgcgcga aatatcgata acagcgttcg tccaattaca
     3061 ctgaacctgt tcaaaggatt tattttttcc agtatcgttg cagttattgc cagctatgct
     3121 tttgcagcat tccaagttaa gcgtatcaat cggttaagga atgcgacgaa agaagtgacg
     3181 aatggtaatt ttgatgtaca gctccccgta catgacaaag atgaatttga tgagttagca
     3241 gatgatttca ataaaatgac gaattcattg aaagaatctc aagcagaaat tgaagaacaa
     3301 gaaaatcgtc gaagacaatt tatggcagat gcttctcacg agatgagaac acctcttacg
     3361 acaatcaacg gacttctgga agggttagaa tacaatgcaa tccctgaaaa tcagcgagaa
     3421 aacgcgatta aattgatgaa aaatgaaaca gagcgtctga tccgtttggt gaatgaaaac
     3481 ttggattatg aaaaaatccg tacgaatcaa atcagtatgg tcattaaaaa attcaatgga
     3541 acagaaacat tgaaaaatat tgttgcacag ttagaagcta aagcagaagc agcaggagat
     3601 acattgacat tgaaagccgt tgatgatatt gatgtttacg ctgattatga ccgatttgtc
     3661 caaatcatgg tcaatattat tcaaaacgcg atacagttta ctgaaaacgg ccaaatcacg
     3721 attactttag aaaaaggcta tcttgaaacg atcatcacga ttgaagatac gggaattgga
     3781 atgtctgaac aacaaatgaa aagtatctgg gatcgttatt ataaagtaga tccttcaaga
     3841 aaaaatacaa aatatggtga atcaggatta ggtttaccaa tcgtgcagca acttgtacgc
     3901 ttgcataaag gaaaattaga agtagaaagc gaactaggaa aaggaacaaa attcgctgtt
     3961 cgtttgcctg acgtagaaat caaaaaagag taaaaaacaa gcggtataaa agatcatgaa
     4021 tcgatcttct ataccgcttg tttttactat aaaatgattg atttagcccg cgggctctaa
     4081 acgatcaata taaggacttt gagcaatagt agcatcatga gtaaagagta agataccttg
     4141 attttcttca tgagctaaat aagaaagcaa gtggagtagc tcttggatct ccaatacact
     4201 aagttcatca aatgtatctt caataacaat attgtttcgc ttggaaagca aggcatgaag
     4261 taattgcagc tttacttttt caaaagacgt caactcagct gccgcacaat taagcaagga
     4321 aggagagata tcaagttttt cttgataggc caaacgatct tgtttgattt ctttttccgg
     4381 tacatccaac aggacatggt ctttaagatt cagataagga aacatcggcc aatttttttg
     4441 gatgatcgca aaattatcca gttttgcaac aagaacctct tttttcaagt gctccaatat
     4501 tttatgctgt ttttcgagag gacacaaaat aacggtcaag tgttcagcat gaatcaaata
     4561 ttcaggtttc attacgtcca ggcctcctac tttgcaaatg atggataaaa aaagtgaaag
     4621 ggaatagact aagaatgaga cagcatccac tgagcccaat aagagtttgg gcaaatccct
     4681 taagtatatc tgtgaaaaaa agatcatttt ggaaatcgat ggtaaaaaaa gtctgttgat
     4741 ccatcggaat tgtaagtccg cttaccgaag tcaagatatc acggatatct ggcagttcag
     4801 tagtctttaa gacaagaaaa ttgatccttt gaagaagcgc ttcataaact gactggaagc
     4861 ttacgatcca caataaaacc aagatacaac aaagaaagag aggaaagaac agctgccaga
     4921 aaataaaaag gaataatttg gttttagaaa gaccagtcag gtgccaattg atcaattcct
     4981 gcttgttttg cctgactgaa tagtaaaaga aaaagataaa aaggaaggca aatagaaaaa
     5041 ataaactgaa tatgcctttt atataaaatc ggctagctaa ttctaaagcc ggaagaagct
     5101 ctgtagatgc agaagcgcca ataaatgaaa gccgatcttg gacttgattg gctaaagccg
     5161 attgcaaatg gtaaagagtc aggcatgcta tcaataaaaa agcagaaaag cttgaaaata
     5221 aacaatagag tattgtctgc tttttgtaat aaattaatcg agcagtagca tagcggatta
     5281 ttttca
//
LOCUS       DACECZ010000143         5201 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.147, whole genome shotgun sequence.
ACCESSION   DACECZ010000143 DACECZ010000000
VERSION     DACECZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.147"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1273..1938)
                     /locus_tag="IWO35_002474"
     CDS             complement(1273..1938)
                     /locus_tag="IWO35_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0317507.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="IWO35_002475"
     CDS             2064..4100
                     /locus_tag="IWO35_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ0317508.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="IWO35_002476"
     CDS             4104..4742
                     /locus_tag="IWO35_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0317509.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="IWO35_002477"
     CDS             4770..5198
                     /locus_tag="IWO35_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317510.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
BASE COUNT         1705 a          798 c          954 g         1744 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt a
//
LOCUS       DACECZ010000144         4968 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.148, whole genome shotgun sequence.
ACCESSION   DACECZ010000144 DACECZ010000000
VERSION     DACECZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.148"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..182)
                     /locus_tag="IWO35_002478"
     CDS             complement(<1..182)
                     /locus_tag="IWO35_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0317511.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="IWO35_002479"
     CDS             517..951
                     /locus_tag="IWO35_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0317512.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="IWO35_002480"
     CDS             974..1294
                     /locus_tag="IWO35_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317513.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="IWO35_002481"
     CDS             1435..1626
                     /locus_tag="IWO35_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317514.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="IWO35_002482"
     CDS             complement(1697..1954)
                     /locus_tag="IWO35_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ0317515.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="IWO35_002483"
     CDS             2112..2375
                     /locus_tag="IWO35_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317516.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="IWO35_002484"
     CDS             2399..2587
                     /locus_tag="IWO35_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317517.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="IWO35_002485"
     CDS             complement(2670..2855)
                     /locus_tag="IWO35_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317518.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="IWO35_002486"
     CDS             3070..3441
                     /locus_tag="IWO35_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0317519.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="IWO35_002487"
     tRNA            complement(3591..3663)
                     /locus_tag="IWO35_002487"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="IWO35_002488"
     CDS             complement(3709..4137)
                     /locus_tag="IWO35_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317520.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="IWO35_002489"
     CDS             complement(4197..4955)
                     /locus_tag="IWO35_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ0317521.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1735 a          779 c          975 g         1479 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctccct
//
LOCUS       DACECZ010000145         4959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.149, whole genome shotgun sequence.
ACCESSION   DACECZ010000145 DACECZ010000000
VERSION     DACECZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.149"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            329..1252
                     /gene="truB"
                     /locus_tag="IWO35_002490"
     CDS             329..1252
                     /gene="truB"
                     /locus_tag="IWO35_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ0317522.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1256..2197
                     /gene="ribF"
                     /locus_tag="IWO35_002491"
     CDS             1256..2197
                     /gene="ribF"
                     /locus_tag="IWO35_002491"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ0317523.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2592..3377
                     /locus_tag="IWO35_002492"
     CDS             2592..3377
                     /locus_tag="IWO35_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0317524.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3390..4241
                     /locus_tag="IWO35_002493"
     CDS             3390..4241
                     /locus_tag="IWO35_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ0317525.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4365..4817)
                     /locus_tag="IWO35_002494"
     CDS             complement(4365..4817)
                     /locus_tag="IWO35_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0317526.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
BASE COUNT         1678 a          811 c         1036 g         1434 t
ORIGIN      
        1 attaagaaac tgaaaaaata aataaaaaaa aacggaaaaa agactggaag aatggacagt
       61 cttttttttc gtttttggga agtgaaaacc aaactattta cctgtaagcc acccataaat
      121 ccaatcaaat ggtgcaaaga aaaaatcttc cattctattc cctcctttaa aaaaaacata
      181 acatgatttc tatttacgtg agcatgaatt ttgttacgtt tttattgccg aaaaataaaa
      241 accgttcatt atgatgtcaa attcgtcagt aattgatgca atgtgttata ataattcagt
      301 taaatcaaaa atgaaatgga gaaaagacat ggacgggtta ttaactttat ggaaagaacg
      361 cggaatgaca agtcatgatt gcgtattcaa attaagaaaa atcttacata cgaaaaaaat
      421 cggacatggg ggtacgcttg acccagacgt agacggtgtg ttgcccattt gtattggaaa
      481 agccacaaaa gttatcgaat atttgacaga ttctgggaaa atctatgtag gcgagataac
      541 tctaggtttc agtacaacga cggaagatcg atccggagaa cgggtagaaa caaaaccagt
      601 agatatgtct ttatctgaag aaaaaataga tgaagtgatg gcttcattta ttggtgagat
      661 cacccagata ccaccaatgt actcagcggt aaaagtcaac ggtaagcggt tatatgaata
      721 tgcccgcaat aatgaaacag tggagcgtcc tgttagaaag gctcagattt accgttttat
      781 ccggacaagt gaattgacaa gagacgaagt gacaggaacc atttcttggc gcttcgaagt
      841 agaatgcggg aaaggaacct atgtccgcac attagctgta gatacaggca agaaacttgg
      901 ttaccctgcg cacatgtctg atctgacccg aacagcaagt gccggtatgt cccagaaaga
      961 tgctctgaca ttagaagaag tagctcaagc tgtgaaaaat gggacaatcg atgattgttt
     1021 gctgccaata gaaaaaggag tgaaacgatt caaacgagta gatattactg atgaagtatg
     1081 gcagaaagta aaaaacggta tgagacttaa ttatactgta tttgcgctcc cagaaatgcc
     1141 gtcagaagaa attgctttgt tttataaagg caaagttgtc agtatctatc agccaaaccc
     1201 gaaagaaaaa aacaaattaa aacctagtaa agtattgaga aatgaggaat aacgaatgaa
     1261 tatcatcaag atcagacatc cttaccaatc agaacaaatt ccagaagacg atgtcgtttt
     1321 agtactagga ttttttgacg gcgtgcatat tggacaccag aaagtcataa agacaggtat
     1381 ggaaattgca caaaaagaag gattgaaatt agcattgatg actttcaatc agcatccatc
     1441 gattgtcttt aaaaagatcg atcctagcag cgtgaaatat ttgactagcc ttaagcaaaa
     1501 agaagaaaaa atggccgctt tgggtataga ttatttatat gaagttgatt ttacttcttc
     1561 ttttgctcat ttagcaccgc aggaatttgt tgatcaatac atcgtagggt tacatgcaaa
     1621 atatgctgtt tccggttttg attatacgta tggaccaaaa gacattgcag atgtcgctca
     1681 ttttcctgaa tatgccaaag gaagattcaa aatcgtcaca gtttctaaag aagaagaaca
     1741 aggtgaaaaa atcagctcta cccgtattcg aggattacta gacagcggac aagtaaaaga
     1801 agcagcaaaa ctattaggtt gcacatatga ggtagaaggg gtagttgtcc atggcgatgc
     1861 acgaggcaga ctactaggat tccctacagc aaatgtcaaa gtaaagagca cagttcattt
     1921 gcctaaagaa ggtgtttatg tatcggaaat aaaaattggg gaaatttggt atccagccat
     1981 gggttcaatt ggacataatg atacattcgg tgaaggacgg gagctgactg tagaattgta
     2041 tattttggac tttaatcaag atatctatgg tgaacatgtc gcagtacgct ggaatcaatt
     2101 tttaagagat caagtgaaat tttctagtgt agaggaattg attgaacaac tcaaagagga
     2161 tgaacaagca acagctgaat atttttctca gcaataaaac attatgcata aaatctcttg
     2221 tttatgtata actagcaatt tcaataaaat gggtgataaa taagcccagg aaaggggctg
     2281 tgacaaaagt cttgtcacag cctttttttc caaataaatg gtttttcaga agtagcttct
     2341 tcggaaataa gccgaaattt cacaaaaatt tgagaagtaa tttttgtaaa ttacttctta
     2401 tttcttgaag ctggccactt ctgtcacaac ctctttcttt atagaaataa aaggacacta
     2461 gcaattcatg aaaacgattg ctaaagcgta tcatttgctt tagactatat atcttggtgt
     2521 acactcactt taagacggaa ataaaagtta tccgtaataa caggtttagg tatgaaaaag
     2581 gagtgggtac tatgtataag gaattagaaa acaaagtcgc agtagtaact ggtggctcaa
     2641 aggggattgg aacagcgatt gctcaacgat tcgggaaaga aaaaatgaat gtagttgtca
     2701 actataactc ggataaagaa ggagcagaaa aagcagtcaa agcaatcgaa gaggcaggcg
     2761 gaaaagcagt ctctgtacaa gcagatgtca gttctgaaga aggaatacag cgtttacttg
     2821 atacggcttt agaaaatttt gggacactgg acgtttggat taataatgct gggatggaaa
     2881 atcagcatcc aacccatgaa cttagccttg aagattggga aagagtatta aaagtcaatt
     2941 tgactggcgt ttttatcgga acgaaagcgg ctttaaatta ctttacagag aatgataaaa
     3001 aaggaaatat catcaatatg tcttctgttc atgaacaaat cccatggcca acgtttgctc
     3061 attacgctgc ttcaaaaggc ggagtaaaac tattcaccga aacggtagca atggagtacg
     3121 caccaaaggg aatcagaatc aatagtatag gacctggtgc tatccgtaca cctattaatg
     3181 cggcaaaatt tgatgatcct gaacaaaaag aactcttgga aagtatgatc ccactaggac
     3241 gcgtaggtga accaaaagaa atagcggcga cagctgcatg gttggcttca gacgaatcca
     3301 gttatgttac aggtattaca ctatttgcag atggtgggat gactttatat ccttctttcc
     3361 aaggtggaaa aggataaggg tgaataaaaa tgggtttatt gattgcattg atcccggcag
     3421 ttgggtgggg gatccagcca ctgattttga aaaagattgg tggcagacca acaaatgaga
     3481 tattaggaac tggtattgga gccgtgctcg ttggcttact tgttcaatta ttcgcttctc
     3541 cagctggtat ttcagtgact acatttttga ttagtttact ttctggtgca ttctgggtca
     3601 tcggacaaac aggacagtat acatctttca acttgattgg tgtttctaaa acgatgccaa
     3661 tttcgactgc attacagtta gtcggaacct cattgattgg tgtcttcgct tttggagaat
     3721 ggtctggaag tatgggaaaa atcattggtg cagcagcaat cattcttctt gtatttggtt
     3781 ctgctttaac ggcaatcagt gatgatcctg gaaaaaaagg cggtatcaca aaaggaatca
     3841 gtctattagc atcaacaagt attggttatt gggtttacag tgcattgccc aaactagttg
     3901 atgcaagcgg actttccatt ttctttccgc aaatgatcgg tgtcttttta ggggcagtga
     3961 tttatgtgat tttccgaaaa ccaaccgcat tttctgaaga caagagttgg aaagcaacaa
     4021 tagttggagc catcttcagt gtgtcagcat tggcttatat tttttcagca aatgaaaatg
     4081 gggttgctac tgcatatatc attacacagc taaacgtagt tatctctaca ttaggcggta
     4141 tgtacatcct gcatgaaaat aagacaccaa aagaaaaaaa attaacgatt atcggattat
     4201 tgctgatcgt agtaggaagc atggtcacgg tctttatttg aggataaaga tggtaaaatc
     4261 tcatttatta aaaaaatatt tgtttgaacc aaacagtaag tacttataaa aaaatagcgc
     4321 tagaatttcc aacatctctg gaatttctag cgctttttaa ttgtttaaga tacaagtgaa
     4381 atatctgtct gcatcaataa tttttcattg atccattgac taaaatttac gacatgattg
     4441 aagttaccac cgattagtat ctccgcatga ttgtctttat aaagatcgta ttgtaaaaga
     4501 cggtcttttt tatttgcgta aacatcgtta tggtactgga ctaattgttc tcggatttga
     4561 ttgattgctg taaaatcacg atactgaagg ttgaaacgca tcgtgattgg tgcatttagc
     4621 atgaatcgct gcatttcttt tgctgcttcc tctgcttgat ctccgtatag gaactcaaaa
     4681 agattacgtt cagaaaattt ccattgcccg ccaacctttg ttccttttag ttttccttca
     4741 tgaagataat ttctgataga acgtgtagta acacctaatt gttcggctaa ttcatttact
     4801 gagtaaaaat ctttcaatgt tcaccctcct atgtgaatgg ttctactgac ttagttgata
     4861 tttgcttaat cacgaaatgt agaaagaacg attttaatta aggtcattgt aaaaatcaag
     4921 tcagaaaatt ctaacttttc tttctctaaa gaaattcgt
//
LOCUS       DACECZ010000146         4824 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.150, whole genome shotgun sequence.
ACCESSION   DACECZ010000146 DACECZ010000000
VERSION     DACECZ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.150"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            106..357
                     /locus_tag="IWO35_002495"
                     /pseudo
     CDS             106..357
                     /locus_tag="IWO35_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="IWO35_002496"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="IWO35_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ0317527.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1765
                     /locus_tag="IWO35_002497"
                     /pseudo
     CDS             1654..1765
                     /locus_tag="IWO35_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="IWO35_002498"
     CDS             1786..1869
                     /locus_tag="IWO35_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAQ0317528.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="IWO35_002499"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="IWO35_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0317529.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="IWO35_002500"
     CDS             complement(2994..3857)
                     /locus_tag="IWO35_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAQ0317530.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="IWO35_002501"
     CDS             complement(3859..4131)
                     /locus_tag="IWO35_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0317531.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4363)
                     /locus_tag="IWO35_002502"
     CDS             complement(4148..4363)
                     /locus_tag="IWO35_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAQ0317532.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..4598)
                     /locus_tag="IWO35_002503"
                     /pseudo
     CDS             complement(4455..4598)
                     /locus_tag="IWO35_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
     gene            complement(4652..>4824)
                     /locus_tag="IWO35_002504"
     CDS             complement(4652..>4824)
                     /locus_tag="IWO35_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0317533.1"
                     /translation="RPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSV
                     CTEIKVLLGIPA"
BASE COUNT         1475 a          819 c          892 g         1638 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgctggt tctgttgcaa agttttaaat
     4621 aaagaataaa atcccttacg gtatctatga tttaagctgg gattcccaat aataccttga
     4681 tttcagtaca gaccgaaaac ccgaagagag tgccttcttt tcgggttttc ttatataatc
     4741 ctcgaatggc ttccatgcct ttaatcgtgg tagaggcagt gcgtaaactt cgatagaatt
     4801 tattgcgtct ctttactgga cgat
//
LOCUS       DACECZ010000147         4795 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.151, whole genome shotgun sequence.
ACCESSION   DACECZ010000147 DACECZ010000000
VERSION     DACECZ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.151"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(81..1889)
                     /gene="pepF"
                     /locus_tag="IWO35_002505"
     CDS             complement(81..1889)
                     /gene="pepF"
                     /locus_tag="IWO35_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ0317534.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2065..3063)
                     /locus_tag="IWO35_002506"
     CDS             complement(2065..3063)
                     /locus_tag="IWO35_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ0317535.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3289..3942)
                     /locus_tag="IWO35_002507"
     CDS             complement(3289..3942)
                     /locus_tag="IWO35_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ0317536.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="IWO35_002508"
     CDS             complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="IWO35_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ0317537.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1406 a         1007 c          744 g         1638 t
ORIGIN      
        1 aaaaaaatac gtcgactccc ttacaagaga gccgacgtat ttttttcgtt tctattcgag
       61 aaaatcaaaa atagaaagcc ttataaacga tcgatcaatt cttctaattc atttagacgt
      121 tgttcaaaca tcgacattgc atcttcaata tatgcagcct gtgtcatatc aactcctgct
      181 tttttcatta cttccacagg ataatcgctg ttacctgctt ttaggtaagc caagtagttc
      241 tctagtgcct ctggttcctg gtttagtatt ttcttcgcca gtgctgaagc agcagaaaag
      301 ccagtcgagt attggaaaac atagtaatta taataaaaat gcggtatccg tgaccattcg
      361 aatttaattt ctgggtcttc ttcgactgct ggaccataat attttgcatt caatttacca
      421 taactatcac ttaggtattc actagtcaat ggcacacctt tttcatcttc cgtatgcatg
      481 aaatgctcga attcagcgaa ttgtgtctgg cggaaaactg ttcctttaaa cccatccaag
      541 taatggttga gtacataagc ccgtacacga gggtcttttt ctgtttccaa taaatactcc
      601 gttaggatat tttcattcgt tgtcgaagca atttctgcta aaaagatgga gtagtcgccg
      661 tacacataag gctggttcga acgagtgaaa taactatgaa cactatgtcc catttcgtgg
      721 acaagcgtaa atagctgatc cagcgtatca tgccaattca ataaaatata tggatttgtg
      781 tcatagcttc ccgaagaata agcaccgctt cgtttccctt tattttcgac aacatcgatc
      841 caacgttcag agaatgcttt ttctacgatg gccatgtatt cttcacccat tggtttcagt
      901 gcttctaaag ctttttcttt tgcttcttcg tacgtaaagg taattggagc ttcccctaag
      961 accggtgtat aaagatcata catgtgcagt ttttccactt ctaataaacg tttccgtaat
     1021 tccatgtatc gatgtaacaa aggcaaatgc ttgtttacca cgtctaccaa agtatcgtat
     1081 acactttcag gaatatgatt attgctcaaa gacgcttctc tggcagagct gtaattacgg
     1141 actttcgctt taaaattatg tccttttata tgtgtgccta aagtagaagc aaatgtattt
     1201 ctaaattgtt cgtaaacact gtacaacccc ttaaatgctg cttcgcgcac ccttcgatcc
     1261 gtgctttcta gcaactgacc atacacgcca tgagataatt ggactatttc accattttct
     1321 ccttcaatcg ttggaaaaac tagatctgca ttattcaaaa cagcaaatgt atcacttgaa
     1381 gcatcaaaga tttcacctgc tccagcaaga agagattcct gctcagcaga taagacatgg
     1441 gctcgattat ctactatttg ctggatataa tggcgataga cttccaattt tggttcttct
     1501 ttgaaatatt gccaaatctg gtcatctgac aattgcaata tttctggttc aaaccaggaa
     1561 acggcttcac taactttcga aaacagactg cttgctcttg cataaagcgc ttggtaatct
     1621 gtatttccag tgtcttgatc gtttttaagg tgcgcatata cataaatgac ttcagtttgg
     1681 cgataaaccc tcaatacgaa ttcaatcgca tttaaaaatt gagaagcgcc ttgatcaagt
     1741 gttcctttgt gtttttcaga ttgttttaac tcttctgata attccaaata tttctcatca
     1801 aactcttggt cgcttgaaaa gatcttggtc aagtcccaag ttaaattttc aggcaattct
     1861 tctcgttttg gcaactgctt tacttccatg aaatccactc cttttcttcc cttagctcta
     1921 tctatcaaaa tagatcatag tctaacatcc acatgtcaca gcatctaact tttctactca
     1981 ttcatgtgtc tgcttccatc aattttagca taactaaatc ttgttttaca tagaaaaccg
     2041 attcctagcc tatttttata cttactattt tttcaaagaa gacaagtgta tgcattcgtt
     2101 aaaaaactgc tgatagacca tctcttcatc aatcatagga aaaagccatt cccttttatg
     2161 ttcttttatt tggcaaagcc atgagttgaa atcaggaact ttctggtttt ccaaggcttc
     2221 ttcgtacaac attcgataaa gaaaaacctg ttcttgtaaa taaaagaaaa accgactttt
     2281 ttgatagatc cacgatgata gatgcaaaag atgtttatga cgcaagtagc attgttcttg
     2341 tatggatact gtattctttt gcttgtgaat aagttggcgt gtcagccatt tcttcccatc
     2401 ttcactcact ttttctattt ttttcatttg gttcatttta atttcgttac tagtgaatat
     2461 ttctttaagg tccctactag aaaaagtcca gcagctactg caaaaggaaa tatgccctga
     2521 tacagtctct gtaatatgat gatacaaata aagcttttgt ttcttgagat cagctttcca
     2581 gcaatgcact cctctttttc tattatagta gcagcaactt ttttctatca atgagaattg
     2641 cgattgtcca agaaaagaat taccaagtat ccaccatact tggtaactgt tattcaaata
     2701 attttgggtt ctttcaataa atctctggtg agacagtcta gaacattgga tttctattgc
     2761 tagattcgcc aacaaaatat ccggcctttg ccgcaacctc ggcaaatagg cttccaaaaa
     2821 tactggttct actgactgtc ccagccaatc catgaagcat tcctttaact gtaagtgctc
     2881 ttttgattct ctttcactaa agaccgaaca gttatctgat ttttgatggg caaaatgagc
     2941 acattttttc tttccctttt tcaaaatcac tctttttcga cagccacgac aataatattt
     3001 ctttcctttt tctgcatgtt tagcaaaaca gcacttatta tattcattca ttgcaagcag
     3061 catttttctc cctctgaact tatttacgtc taagaattta tttacacgaa aaaagagggc
     3121 gtgaaaaagc tattctgcat caagtaataa gaacagctaa acaaaaatag ttcccaatat
     3181 ttttcgtttg gttgggcttg ttaccgcaga tgcaagcttt tgaacacatt tatacgaaaa
     3241 aaagactgcg acacaatttt tgtcacagtc tcttactata atgctgattt atacaaaata
     3301 aaatttcgtt cgctcgatag cattacgcgc catcagacat tgtccatatt cattcaatgt
     3361 ttctggtgta cgatcgctct tttcagcaaa ctccaaaatt cgagcaatct gattctctac
     3421 atccgcttgg ttgagatttt ccatccagaa gtgaacgact aggtaatagc gctcttcgaa
     3481 taaatacaaa tcagtccaag cagactctag ttcgacatct gttgcaagtt ggatcacttg
     3541 ttcaaaatct tcaaaagcaa aagttgcttg tctttcctgt tcttctaggg cgcttccaga
     3601 taattcttcc tccggatcgc tgccaataat ctgctgacga agaaagtcac tcacatcttc
     3661 tgaatcaaca gtagaatctt ctgattgaga aatttcttca tccggaagat ttttactgat
     3721 aaacagttct aatccgtctc cttttggcaa aacttggaaa gtgacagctt cgcttcctcg
     3781 gaactcctct tcgatatcaa cttcttccaa aatactataa aagaaatttt cgatttcttt
     3841 gtgattgcca agtaaatcca aaaatgaaac gccccgttcc tctaaatctt catgaccaat
     3901 tagtacacgg atcgtatttt cattaatatg ttccatttcc attttgctac acctcacttt
     3961 gccacaatgt ttaatataga ttcattgtag cgaaatcagc ttaaatgtaa agaaaaaaaa
     4021 tctcgctgtt aagcgaattt aataaaacca gcggattttt caacctttca agctatctta
     4081 tttaactata acatttttgg cagacgaata ctatatagcc gacttttcag ctaattttcc
     4141 ctctctattt ttcgtgcaat catcatcttc tactaccttt ttatacatct aatcaagaat
     4201 actttttgta aaaacaaaaa aacttggaaa aactatcttt gtaatagtct tttccaagtt
     4261 tcgtattatc cttctataat ccagccatca attgtgcttg acgtaattct aattgtcgta
     4321 catcgcgcgg taggaaccga cgaatttcat cttcattaaa accaacttgc agacgtttat
     4381 catcaatcat gattggacgg cgaagcaatc caggattgtt ttgcacaagt tccaataatt
     4441 cttgtaacgg cagatcatct aaatccatgt tcagtttttg gaatactttg gaacgtgtcg
     4501 agatgatttc ttctgttccg tcttctgtca tttgtaaaat cgcttttaat tcagaactgt
     4561 tcaatggttc tgaaaaaata ttacgttcaa caaatggaat ctgatgttct tgcaaccacg
     4621 cacgagcctt tctgcaggaa gtacaacttg gggaagtata taatgttaac aacgtgcacc
     4681 actccttttc gtgataagaa tcactttttt gtaacagaag atcataataa tctcccatct
     4741 gtttctatta tacataatca acttttaaat agctaccttt ttttcataat ttttt
//
LOCUS       DACECZ010000148         4511 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.154, whole genome shotgun sequence.
ACCESSION   DACECZ010000148 DACECZ010000000
VERSION     DACECZ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.154"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            524..1435
                     /locus_tag="IWO35_002509"
     CDS             524..1435
                     /locus_tag="IWO35_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317538.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1922..2188
                     /locus_tag="IWO35_002510"
     CDS             1922..2188
                     /locus_tag="IWO35_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317539.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2260..2601
                     /locus_tag="IWO35_002511"
     CDS             2260..2601
                     /locus_tag="IWO35_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317540.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2656..3075
                     /gene="ssb"
                     /locus_tag="IWO35_002512"
     CDS             2656..3075
                     /gene="ssb"
                     /locus_tag="IWO35_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0317541.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3152..3448
                     /locus_tag="IWO35_002513"
     CDS             3152..3448
                     /locus_tag="IWO35_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317542.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3463..3894
                     /locus_tag="IWO35_002514"
     CDS             3463..3894
                     /locus_tag="IWO35_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317543.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4076..4381
                     /locus_tag="IWO35_002515"
     CDS             4076..4381
                     /locus_tag="IWO35_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317544.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT         1659 a          710 c          951 g         1191 t
ORIGIN      
        1 ggtttttgac cattttctct caacgtggaa aaaccgccac ttatgagaga aaatggtcaa
       61 aaacctaagc gggaagtctc cgaataacat accaagcgcc gacagccaac agataaagaa
      121 tacactacta aaaatagtag tagcataaaa tttaaaaggg aaaaaatcaa cccctacccc
      181 agtcttgggg tctcaaaact aactcccccc taaccccctc tttcagcctc ttttgaaaga
      241 gggggagaat caaccccacg gccaacgccc cagtgttctt ggggctgtcc agaacagaaa
      301 cttgcgagag agtgggggta aaacaagtgt agaggaaact tgaagacttg ttcgaacaga
      361 aacttgcgag agagttggag taaaacaagt gtagaggaaa cttgaagact tgttcgaaca
      421 caaacttaca agagagggga gtaaagcagt tgcagaggaa tcaggaagat ttgttcgaac
      481 agaaacttgc aagaaagcat tattaaattg aaggtgatga aatatgaagt atttattgca
      541 tggctatttt attgattatt ctactcgcaa tagtagtact ttagcgattc tatgtttgct
      601 tcatgaattg agagtggtta ctagaaaaca gctatacaaa ctattgaatt ttgacagatc
      661 aattagtaaa caaggagtaa cgaatgcttt agtgagatta tatgaaaaac agttaatcga
      721 tcgaaaaaaa tatggaacag aaatgtatta ttatttgacg aaagagggac atcaaagcat
      781 aggtgggtat tatacgttgc cgaaagttcc tgaatataat ttgaatcacc atttacaaat
      841 taacaattat ttgattaaag gaatggcatt ggcaaaagac cacccacatt taaaacggat
      901 tatttcagaa agacgtaccg tctatgaaac gaaggattta aatcctaaga caaaaggaag
      961 aaaatttttt gtgccagatt ttatttttcg ttttcgagac aaagaaaatc atgagatcaa
     1021 ttggtccttt gaaattgagc taacgttgaa aacacgtcgt cgctaccgtg aagcgatttt
     1081 tccgaaatat attcgtgagt tgcgaacacg tcctgaatca catttaattt atgtgacacc
     1141 tagtggcgca attcaagaag aacttcatgc gtttaagcgt atttttttca agcgcgaaac
     1201 tgaaaaagat gcgagtcaac aagaattgtt tgagcggttg catatttttt cagctttaga
     1261 gtttgaagag ggattaaaag cattattaga gaatgatcca gcagtcaatt ggcggacagt
     1321 agaacgagaa aaaatttatc cgaatggcgc tgttcaaagt cgtttgaatt tatttgaaga
     1381 atcaactgaa aatgaggaaa aaccaaaagt gaagtttacg cctgaagtat tgtaaaagag
     1441 ggtgttcgct tcgctctttt tttgtgccag tttgctggca ccgttcagac gcaagcgtct
     1501 gaaccggctc gtttttagga aggactaact tgtttgattt atccgttgtc aaagaagtgg
     1561 acgttcgaga gtccctaatc gtatagcagt cattcggcga gtataaccta aaacataacc
     1621 tgccaattgc caagggtaaa atgcacgctg cgcgcccttg gcaatcgtca gaacatgttt
     1681 tcgagccggt tatgccgaac gaccgctata cgaaaagggc ctctaaacag aagtccactt
     1741 tgatccttga caactgaaac ccaaaatcaa acaagcaggc cttcgtctaa aaacgagccg
     1801 aatgaagacc ttgggggttg cacccccaaa cccccgtgcc cccgacacac taccgaaaag
     1861 gtgggggtgg gtggcgaaaa aatcaaataa aacattctat tcaagaaagg aagaaacaac
     1921 catgacaact aaaaacgagc aactgaaaga acaggcaaga aaagagttgc aagaaagagg
     1981 tttgattatt gaggggagct ttgaaggaga ttttgaaacc tatattggtt gttatgcaag
     2041 accgataaac aaacctacag ccttagaccc aatgaacgaa caagaagtaa aagaacaaga
     2101 aaaacatgca gtgaatggtt ttccgcaaga tttcactgaa tggtatgagt gggaaattaa
     2161 caacgggaag cttgaaaact tcttataaaa gtcagatgac gtgcaaagag aatgattctc
     2221 tttgtacggt aaaaatataa agaaagaagg aaaaagaaaa tggcaaaaaa cacaccttat
     2281 caagtaggag acatttttta tagtagttgg ggctacgaac aaacgaacgt gactttttgg
     2341 caaattgtga agttaacaga aaaaacagca tggtttcagc ctattcaaaa gaaaacggtc
     2401 gagcattatt cgtccatgag tggaaaagtt gttccaatga aagacaagtt tatagagtat
     2461 cctttagcaa aaactaaaga cagtattgta aaaaaacgaa ttgatccaga aacctatcct
     2521 gatatgttat gtggttatcg tagttgggac acgtttcata gatataaaaa tcaagctgtt
     2581 tatgaaagta gttattatta aggggatagt ttcccctttc aaaaataata aatagaagaa
     2641 tggagagaaa aaatcatgat gaattcagtg aatttagtag gacggttaac aaaagaagtg
     2701 gatttaaaat acacaccaaa aggcaatgcg acaggcacat ttattttagc tgttaatcga
     2761 aattatacga atgcaaacgg cgagcgtgaa gcagattata tccgctgtgt catttggcgg
     2821 aaagctgcgg aaactttagc aaaatatact ggaaaaggaa gtttgattgg cattaatgga
     2881 cgtcttcaaa cgaggagcta tcagaatcag caaggacaga ccatgtatgt tacggaagtt
     2941 ctagtgacag atttctattt gttagagagt aaagaaatca atgagcaacg gtcaaaatca
     3001 atggcgaata atcaaaataa ttcttcaatt ccaggaaatg agattcaaat ttcagatagt
     3061 tatttgccct tttaagtgaa aaataatttt gttctatacc tgatttcttc aggtatagaa
     3121 caaaaaataa atagagatga ggcgattttt tatgaaaatc attgacagtt acgatcaacg
     3181 aaccatgcaa tatttgggct cgtttgatta tacagatgaa aatattatta attatgttgc
     3241 aagtttacct ccttatcaaa gcgttcgctt ggtagatttt gcaagtgatg aattggtatt
     3301 aacaacgatg ggcaactttt tagattatgt cggagacaaa aaatggttgg aaaaaatttt
     3361 gtctaaattg attgcgaaac aaacaggaga agaacccata aatgcgattc aattgttcaa
     3421 tcgatatgaa caagaaaaca atgattaaag aggagagaaa aaatgaaaag attaaaacga
     3481 agtattttta ttgtgctagc attgattgat tttgttggga caggttgtga agcaattgac
     3541 gttcaaaaag ctcccttatc ggaaataaaa caagcaataa caaaacaaga tgaagaacaa
     3601 ccgctaaata cacaagtaga aatagtgaat caaaataaac cgagtttttc tgaagaagat
     3661 ttttcgttag aaaatggaac caggcaattt ttttcagatt tggatcgttt aaatcgggta
     3721 ggtgtagcaa gtgcactttt gcataaaagc aggatgccaa cagaagaacg agaccgatta
     3781 tacattaagc caactggctg gaaacaaaag aaaatgaaga atggcgatta tctttataat
     3841 cgttgtcatt tgattggcta tcaacgtgcg acaagtttcc ccataagtat gtaatacgaa
     3901 acgggaggat taatcttaag attaatcaca actaacctat ttgacagttg gaatgaaagc
     3961 tatcatgtct gctgaatcaa ctggtctgat actcctacat gcaataaagt ctactaggaa
     4021 gttattgtat gaagctaggt aaaggcggca gaacaatttc taagtgtgga atcatatgaa
     4081 gttggatatg ccgggacgct ataaattact cagggtgaga ctgtttttaa ttaaactgac
     4141 gaacttgcga atgtacgggt ctataaccga cttatattcg aaagatataa gattgcgaat
     4201 gcaaagaaca ttatcgaaaa gtaagaatgt tggtcatgaa attcgagatg gaagactagc
     4261 ggtgtatctg tcaagagttg gtaaaacatg tttgattgat tcagatgaga gtgggaatgc
     4321 gacaaaacgt tttacagaag aaatagagca actagcagaa ctgaatatac gatattttta
     4381 aatactttag cggagaataa aaaaatcctc tacagaaaac tacggagggc aacatgtgct
     4441 attgtttcaa caatacattt ttaattttta ccataacaaa aaagtacttg aaaaaaaaca
     4501 gaaatatgtt a
//
LOCUS       DACECZ010000149         4446 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.155, whole genome shotgun sequence.
ACCESSION   DACECZ010000149 DACECZ010000000
VERSION     DACECZ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.155"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            156..1862
                     /locus_tag="IWO35_002516"
     CDS             156..1862
                     /locus_tag="IWO35_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ0317545.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="IWO35_002517"
     CDS             complement(2020..2922)
                     /locus_tag="IWO35_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0317546.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3623)
                     /locus_tag="IWO35_002518"
     CDS             complement(2919..3623)
                     /locus_tag="IWO35_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ0317547.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="IWO35_002519"
     CDS             complement(3654..4295)
                     /locus_tag="IWO35_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0317548.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1377 a          948 c          768 g         1353 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagat
//
LOCUS       DACECZ010000150         4388 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.156, whole genome shotgun sequence.
ACCESSION   DACECZ010000150 DACECZ010000000
VERSION     DACECZ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.156"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            40..270
                     /locus_tag="IWO35_002520"
     CDS             40..270
                     /locus_tag="IWO35_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ0317549.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            367..1668
                     /gene="murA"
                     /locus_tag="IWO35_002521"
     CDS             367..1668
                     /gene="murA"
                     /locus_tag="IWO35_002521"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0317550.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1681..1857
                     /locus_tag="IWO35_002522"
     CDS             1681..1857
                     /locus_tag="IWO35_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0317551.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            1992..2288
                     /gene="yidD"
                     /locus_tag="IWO35_002523"
     CDS             1992..2288
                     /gene="yidD"
                     /locus_tag="IWO35_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ0317552.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2352..2735
                     /locus_tag="IWO35_002524"
     CDS             2352..2735
                     /locus_tag="IWO35_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0317553.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            2904..3788
                     /locus_tag="IWO35_002525"
     CDS             2904..3788
                     /locus_tag="IWO35_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0317554.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            3905..4138
                     /locus_tag="IWO35_002526"
     CDS             3905..4138
                     /locus_tag="IWO35_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ0317555.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4226..4306
                     /locus_tag="IWO35_002527"
     tRNA            4226..4306
                     /locus_tag="IWO35_002527"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4260..4262,aa:Tyr,seq:gta)
     gene            4313..4384
                     /locus_tag="IWO35_002528"
     tRNA            4313..4384
                     /locus_tag="IWO35_002528"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4345..4347,aa:Gln,seq:ttg)
BASE COUNT         1488 a          687 c          979 g         1234 t
ORIGIN      
        1 attagttatt ataaaacaat cgttagaaag gacgtcatta tgcaagtata cggaattgat
       61 gcggtgcttc gaatcgtgag tcatttggcg tttatctact tagccttctg gtctttacgt
      121 tcgttacgca ttgaaacttt tttcagacca atgcataata cgcaaattag aatggcaatc
      181 gtactttttt caattttttt agggtacact gcaagtaatt ttttcttaga agttatcgct
      241 ttatgccgaa atcttttcgt tagcctgtaa attttaaaaa aaggtactat tttaaaagca
      301 aggatgttat aatagaaaag attttgagta agtgtatgct cattggaatt ttttggaggg
      361 aacaacatgg aagagatcat cgtaagaggt ggcaatcaat taaatggaac agtaagaatt
      421 gaaggagcta aaaatgctgt tttgcctatt ttagctgcta gtttgttagc agaagaaggt
      481 attacgacat tagacaacgt tccgattctt tctgatgtct tcactatgaa tcaagtgatt
      541 cgtcacttaa acgtagatgt agattttgat gaacaaaaga atcaagtaac aatcgacgct
      601 tcaagacaat tagaaatcga agcgccttat gagtatgtta gtcaaatgcg tgcttcaatc
      661 gttgtgatgg gtccgctact tgccagaaat ggacatgcaa aagtagctat gcctggtgga
      721 tgtgcgattg gtaaacgacc aatcgatttg catttaaaag gatttcaagc tttaggagca
      781 aaaattattc aaaaaaacgg ctatatcgaa gcaattgcgg atgaattgat cggtaacacg
      841 atttatttag atttccctag tgtgggtgct actcaaaata tcatgatggc tgccgtaaaa
      901 gcaaaaggaa caaccatcat tgaaaacgta gctcgcgaac cagaaatcgt tgatttagca
      961 aacattttga ataaaatggg cgctcaagtc tatggagctg gtactgaaac aatgcgtatt
     1021 gaaggcgtgg atcatttaca tgcagtcaac cactcaatcg tacaagatcg tatcgaagca
     1081 ggtaccttta tggttgctgc tgcaatgaca caaggcaatg tgctgattgc agatgctatc
     1141 tctgaacata actgcccatt gatttctaaa ttgatcgaaa tgggtgcaga aatcattgaa
     1201 gaagaaggcg gagtccgtgt catcggacca aaacatatct tacctacaga tgtaaaaacg
     1261 atgcctcatc ctggtttccc tacagatatg caagctcaaa tgacagctat ccaattagtt
     1321 gcagaaggta caagtgtggt gactgaaaca gtgtttgaaa atcgtttcca acatttagaa
     1381 gagatgcgcc gtatgaacgc tcatgtcaaa atcgacggaa atgtagcaat catggatgga
     1441 aaccatgaat tgcaaggtgc agaagtatat gcaacagact tgcgggcagc agcagcatta
     1501 gttttagctg gcttgaaagc aaatggaatc acacgtgtgc gtaacttgaa ttatctagac
     1561 cgtggatatt ataatttcca tatcaaacta cagcaattag gtgcagacgt cgaacgtgta
     1621 gacatggatc aaacttctgc tgaaaaaaca gcacaaacga ttgcttaagg aagtggctga
     1681 atgagtaacc aacgatatat cttattaact ttgataaaga ttttagtggt gatcttattg
     1741 cttatccttc tttttgtggc cggaacgatg atcggatatg gtgtcatcgg tggcggcaat
     1801 ccatttaaag tattccagcc aagtttgtgg attcatattc gagacttttt tcattgataa
     1861 ataagaatca cagtggttct agtcgatatg aagtgagcca tgaagcagaa catgataaaa
     1921 gaaaacagaa gggaacgttg acgttagtcg cgatcccttt ttttagtaaa agaaaagaag
     1981 gaatctaatt gatgaagaag atattgattt atttagttag aggatatcag cgctttattt
     2041 cgcctgcatt tccacctagc tgccgttatt acccaacctg ttccaattat atgataaaag
     2101 caattcaaac ccatggtgcg ctaaaaggga cggtaatggg gactgcacgg atcttacgct
     2161 gtcatccttt tgtcaaaggc ggtatcgatt atgtaccatt gacattccga ttaacgcgaa
     2221 ataagaaaga taaaaaagaa ccagattata aaaaggttgt gaaaaatacg ggtagcactg
     2281 agcgataaag aaaaagagga aatttattgt caaagtaaca actccgcaac tacttatatg
     2341 ggggacaaaa aatgatcgaa gaactaaaca agaaagattt accatggtcc ttgttattag
     2401 atgcagatcc tgataaagaa aaagttcaag tatatgtttc cagagggagt ggctggatat
     2461 ggaaagaaaa agaaaaaaca ataggtgtac tgatctatgt ggcaagggaa aaagaatacg
     2521 aaatcgtcaa tgtagcagta accccaacgt atcaaggaaa ggggattggc ggaaaattac
     2581 ttgagacagc ttttcagaag ctttcacagc tagcttcaaa ccaaactcgg atcattatac
     2641 gtactggcac tactagtagc gctgcgttac acctctatca aaaaatgggc tatgtggaga
     2701 ttggtcgagt caaagactat tttatccatc attaatctga gcccattttt gaaaatggag
     2761 atcaattaag ggatcaagtg attttagcaa ttcacttacc taaaaatagc tttaactaat
     2821 aaactttcaa ttcgagaaaa tgctttcaaa tgctaaatag atacattaca atagctttag
     2881 caaacaaaaa ggagaatatt tttatgggaa tattagtttt tgattttggt ggatcggctg
     2941 tcaaatatgg ttgttgggat ggcaaagaga ttaaaggtaa gggacaattt gcaacgcctg
     3001 aatcttggga agaaatgaaa acgcaattat ttcaagttta taaaaaaata ctcgtttctt
     3061 ttgaagtcga aggaattgga atcagctctc ctggagtagt gaataatgag aaacaggtga
     3121 ttgagggaat cagtgcgatt ccttatattc atggatttaa tattttccga gatttggaaa
     3181 ctctgtttca actcccagta acgattgaaa atgacgccaa ctgtgccgga atggctgaat
     3241 tttatgaagg tgctgcccgt gattatcaag atgttgcttt tgttgtggca ggaactggga
     3301 ttggcggggc gttgttcacg aaaggacaaa taaataaagg ggcacatctt tacggtggag
     3361 aatttgggtt gatgttctta gatggcgata agacattcag taaacttggt acagcggtac
     3421 agatggcttg gcgttattgt gaacgcaaag gtctcgataa aaatgcttat accggaaaag
     3481 atgtctttga attagcagaa actggagatg caatagcaaa agaagaagtt gagagttttt
     3541 atacgtatct tacaaaggga ttattcagta ttcaattttc gtttgatcca gaagtgatcg
     3601 tattaggcgg cggggtttct gcaaaagaag ggctcattga tgagatcaaa agccgaatga
     3661 agaagctgac agaacaattc gatctgcatg atttcgaacc gcagatttta ttatgtgagt
     3721 acagaaacga tgctaatctt gtaggtgcag cagcaaacta tctcgctgta cgtcaaggag
     3781 agaattaaga gctttagaga aggtgcagct tgcataaaag aagagaatga aatgaatagg
     3841 agatttttac ctaaggaatg ttttatttgc tgcagtatga taaaatatct gagaggtgaa
     3901 aatgatgtca aaaaaaaata aagatattga agtgcgtata gaagaagtga aaaaagatat
     3961 caaaggtaag aattatgaag tcactcagtt atttataggg aaaaaaatga ttggtgagat
     4021 cttagcctat gggccaaaag aatatgaaat tttctttggt gaagaagatt ttggaaaaga
     4081 aaaaagtctt gaaaatgcga tagaaacagt gattcgacac tggaatctgc acgaataaaa
     4141 aaatatcaaa aaaactttca ataaagggtt gcaaaaatct ggaaaattag gtattataac
     4201 taagtcaggt tgatacaaac ctttcggagg agtagcgaag tggctaaacg cgacggactg
     4261 taaatccgtt ccttagggtt cagtggttcg aatccactct cctccatttc attggggtat
     4321 agccaagcgg taaggcaagg gactttgact ccctcatgcg ttggttcgaa tccagctacc
     4381 ccagtata
//
LOCUS       DACECZ010000151         4307 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.157, whole genome shotgun sequence.
ACCESSION   DACECZ010000151 DACECZ010000000
VERSION     DACECZ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.157"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..538)
                     /gene="sgrA"
                     /locus_tag="IWO35_002529"
     CDS             complement(<1..538)
                     /gene="sgrA"
                     /locus_tag="IWO35_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ0317556.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTE"
     gene            complement(734..1573)
                     /locus_tag="IWO35_002530"
     CDS             complement(734..1573)
                     /locus_tag="IWO35_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0317557.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            1758..2645
                     /locus_tag="IWO35_002531"
     CDS             1758..2645
                     /locus_tag="IWO35_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ0317558.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(3229..3924)
                     /locus_tag="IWO35_002532"
     CDS             complement(3229..3924)
                     /locus_tag="IWO35_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ0317559.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(3908..4306)
                     /locus_tag="IWO35_002533"
     CDS             complement(3908..4306)
                     /locus_tag="IWO35_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0317560.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1425 a          854 c          639 g         1389 t
ORIGIN      
        1 tttctgtgct actttcactg ctcgtactct ctgtactgct ttcaccgctc gtgttttctg
       61 tactgctttc actacttgtg ctatccgtac tgcttggcgg tccaacaact gtccaactgc
      121 ttggtttcaa attctccgtg atcgtcatct gtccatcata aaaaacaatc aaatcaactt
      181 tatctttact cttgatctca tcaatcgata gttctgcaga aatcattatt gtatactcaa
      241 taccagcggt ccattgtacg tcttttaaat aaacttcccc aataactttt tctgaacttc
      301 cattaccatc ccaaccatat gttagaacag catgtacatc actagcattc acgcttttgt
      361 ttggtttgac tggaatagta atgacttgtt gtgtcatgtc ataaacaatc cgatcagttt
      421 tcacagtaaa tgaaagattc tcatttgccc gttcatttgc agaagtactg attccaatca
      481 ttgctgcaat tatactcagc ccaagaataa caaataatac ggtcgctgtt ttcttcattt
      541 ctatcactct ccatataatt accttctatt tgataaagga attatataaa atgaaaatga
      601 tgagaacacg acagtcagtt gtgaaaatct aacaaaagat gtcgtgtatt cgcttgaatt
      661 tgtttacttt ttcgtttaat caatgacaga cttaaaaaag caagaccgat atcagccttg
      721 ctttcttcat cttttattga taaacagcag tgtatttctt catccgcttt gttttatatg
      781 catagagtaa caaaccaaca atcaagacta acaagaataa tgtagttgat atagatgctt
      841 cagccccaaa gtttccccca gtaattattg tattttgcga ttggatcaga gtggagaaaa
      901 aaccttgttc ttgcgttccg ctgactggta tacccaaaat agctccttgt gtccaattcc
      961 aagtaatatg agcaccaata gccgtaaata aacttcctgt actgtagata agtgcgctga
     1021 aaaagcaacc tgctaaaaag atattgaaaa ctgccagaaa tgtcatgtta ggattcaatc
     1081 cgtgcaaaag agcaaataat gcagcagatc caatcattgc tattaacgga ccaaatctaa
     1141 gcatctgatg ctgaaaaaag ccacggaata aaagctcttc tgatgccccc actgcaataa
     1201 ataaaataaa actcgttatc agcggcacaa tgtttgcgtt tcctgaatct aaccgatacg
     1261 cccccgtcaa atataatgga ctagtaataa gcagaaacac tccaattcca acaacgagac
     1321 ctcccgctaa atacccccag tcttttcctc gaacagaaac accataatct tctattcttt
     1381 ttccttcgac cttactagca agaaataaag ctagtaaaaa aacagctaaa tgtccgaccg
     1441 taccggcaat tgctccaatc cattcactat ttccaataaa catatccgca agtgcattat
     1501 tgacaagaat tcctaaaaca aaaccaacta agactattaa aacagatcca atcaaccgaa
     1561 taatcctgat catctttcat ccctccttta tattaagttt aacaaccctt ttctttcttg
     1621 tctcgtgaaa actatgtaaa tgttctccat catagagaga atagattttt acgaactaag
     1681 aatatctaca gcaaccttac gaaaatgaaa gactgtctcc ctaattcttc tgttagactg
     1741 tttttaataa ggaaaggatg ggaaattttt tgaaaaaaca actactaatt atgtgccttt
     1801 cagcatgtgc aattttacta ttctcaggtt gtagcaagtc caatgaaacg attgcaacgt
     1861 ttaaaggtgg aacacttaca gttcaggatt tttatgataa cccaaaagtt aaatcaaata
     1921 atcaaacact attaaaaaaa atgattgtct tcaaagcatt tgatgatgta tacggaaaag
     1981 agatttcttc aaaggaagta acaaaagaat ataatgaaca aatcaaaaaa ctcggtccaa
     2041 actacaagga acaattaaaa gcagttggcc aaacagaaga gacatataag aaactcttta
     2101 aacagatgct ggctttccaa tacggtttga aagccaatgt aaaattaaca gataaagatc
     2161 tagacactgc ttggaaagaa ttctatccag aagttagtac acagatcatt ctattttcga
     2221 ctaaagaaga agcagataaa gcaaaaaaag aagcaaatga aggggaaaat ttctccaagc
     2281 ttgttcaagc ttatggaaaa aataaaacat taaaggaaac tgatggaaaa atgaattttg
     2341 attcaaccaa tccagagatt cctactgaag taaaaaaagc agcatttaaa ctaaaaaacg
     2401 gggaagtttc tgatatcatt cctgtaacag atccaacgac ttatcagcaa agctattatc
     2461 tagtaaaaat ggtaaaaaaa caggataaag gttctaataa agacaaatac aaatctgagc
     2521 tagaaaaaat tgctaccgaa gcaaaaactc ttgatacgga attcatggat aaaacaatcc
     2581 gtaaagtgat gaaaaaagac aatgtaacta ttaaagatcc ttatgtaaaa aatatattca
     2641 aataaacaaa tttgacccaa ttggggatgt ggcaaaattt gagaagtgat ttttggaaat
     2701 tccttcttat ttttgaggca aaccactttt gccacaactt catcggggcc tttttttatt
     2761 tgtaccaaaa caaatcactg gatttgttac aatcatgagg atataattta aaaaagtttt
     2821 ttatttctat cacatgaatc aatgcgccat taagtagatt ctttcatgct tccctatttt
     2881 ttcacttgcc gtatcgcttc aaaaacaact atcataaact caaactcgac tgaaaagtta
     2941 ttttaaaaaa gactaaaaat atcccttctg gacttgttcc tcttattctg ttatactaga
     3001 gaaacacgaa agatattttt atgcgacact tttcatttgt ttttatgaaa tttattcaaa
     3061 actggcgtaa cttcagagtt acaaggatgt attagaggtt agggaataca tcgtttaaat
     3121 gagaaataag taatagtcac ccaaacttta aattaatgaa tattgaatcg tcagatagcc
     3181 tcctcgagtt ttcgagaagg ctatcgttta ttttatggag aaattgattc agaagtaatc
     3241 agcgaccttt tttagttcac caatcgtttc tgcttggaaa gtttgcaccc aatcttcttg
     3301 gaagcccctc actgctttat caatcgaggg catctctaat acttgatcta gtagctcttt
     3361 acctaacgtt atcgcatgag ctccagatcg aatggctgta gtgacttgtt cacaattttt
     3421 gaaactagct gctaatattt ttgttggtct ttgcgtacgt tcgatctcag tccgcaacga
     3481 atcaatcact ttcatagcat caatgccttg attttgcatc ctgttataat aaggtgcaat
     3541 ataatctgct ccattattaa tagctaaata cccttgtaat tctgtgtaaa tagctgtacc
     3601 ggtaatcaaa taaccttctt ttttcaactt tttgatcgct tgaaggcctg cttgattcac
     3661 cggcaccttg ataaacgtat cttttcccag ttcttctatg ataactcgag catcctctat
     3721 catttcttct tccgttcttc ctattgtctg aatatggata ggaatctgtc cgattatttt
     3781 tttgatttct ttcatatgcg agaagaagtt tgacgctcct tcttttttca gaattgtagg
     3841 atttgttgtt acgcctgtta aattcaaaaa ctgttgacaa gaataaatac tttttgtatt
     3901 tgctgtatca agcatgattt ccatatagct ttcccccatt ctgatcctct tgatcgattt
     3961 taatgattgt cccctttctt aaatgcggca tttccttatc ttctacatac attgttcctg
     4021 gaagttccgg ctcatgagat ccatcaaatt ttatcgtgat atgtcctaat tcattcaaat
     4081 tagtttggac aacatctcct actgctgtga ttacatacat ttgtttatca aaacagagct
     4141 tcatatcaca agttatttct tttcttacct gattcaattc aatcttataa caataatctg
     4201 ttaatgtttc cggtgcttca tctccaaata aaatcattat tttttctttc ctgaatactt
     4261 ctgcttctat accaacatct gttacaaccg tttcaaagat cgtcatc
//
LOCUS       DACECZ010000152         4251 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.158, whole genome shotgun sequence.
ACCESSION   DACECZ010000152 DACECZ010000000
VERSION     DACECZ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.158"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            119..820
                     /locus_tag="IWO35_002534"
     CDS             119..820
                     /locus_tag="IWO35_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0317561.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1392..2408)
                     /gene="tsaD"
                     /locus_tag="IWO35_002535"
     CDS             complement(1392..2408)
                     /gene="tsaD"
                     /locus_tag="IWO35_002535"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ0317562.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2405..2869)
                     /gene="rimI"
                     /locus_tag="IWO35_002536"
     CDS             complement(2405..2869)
                     /gene="rimI"
                     /locus_tag="IWO35_002536"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0317563.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2876..3418)
                     /gene="rimI"
                     /locus_tag="IWO35_002537"
     CDS             complement(2876..3418)
                     /gene="rimI"
                     /locus_tag="IWO35_002537"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0317564.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3402..4226)
                     /gene="tsaB"
                     /locus_tag="IWO35_002538"
     CDS             complement(3402..4226)
                     /gene="tsaB"
                     /locus_tag="IWO35_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ0317565.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1263 a          895 c          735 g         1358 t
ORIGIN      
        1 aacaaattat atttgtaaag tattaaaact tgtttctaat gttggtatgt taaaacttta
       61 caaattcttt aaaaaaagtg acaataaata tagtgaacaa taaggagagg actttttaat
      121 gcgtgttaat aaaatcgatt ttcacttttc aaaactcaga tatcaacctg attttgaaca
      181 tttctcggat gaagagttcc aacagatcaa ggaacatact gttcttcgtt cttataaaaa
      241 aggacagatc cttttcgacg atggtgatcc cagacaacgg ttgtatatct tgaccaaagg
      301 actcgttcgt ttagaacgtt atgatgaaag tgcaacttat tactactatg actatgtgaa
      361 tacaaataac ctttttccta tgtcaggaat gtttggagaa gagacctaca cttatacagc
      421 tcaagcgatg actgatattg aaacattcta ccttcctgtt tcctactatg aaaaattagc
      481 acgaaaaaat cccgaacagt tgatctatat gatacaaaaa atttctaaag tagttgaaat
      541 acatgaattg cgtatccaaa tcggattgac ctctagcgcc tttgaccggg taaaacaaaa
      601 tttattcatt ttgaaagaag aattgggcgt accaacaaaa gatggtcata tcaccattcc
      661 ttatccgatc accttaaaag aattagcagt gaatagcggc acgacacgtg aaactgctgg
      721 acaagtaatc aaacaactac gtgattccgg actgttagat tatgacaaaa agcgttttac
      781 tttctgtaag acagctacta ctccagctat gagagaataa cgcgaacaaa aataagcagt
      841 gaccctgata tttatcaggg tcactgctta ttttcctccc taaaacttct gaaagatccg
      901 gcagttttag atcactgggc aagactcaaa gattttctat gtgtgtttca gttatgtaat
      961 attcactgtt tgcaaaaaat actgatagat taactagagg aagcgtcttg tcctcatccg
     1021 acctagatta ttcgatattc ctcgacaata aacgattaat cagtgagggc aatcagagga
     1081 acggataatg atgaataagc tgtaaaaaat agggctttga caaaagcgtg tcacagccct
     1141 tatttcgatt aaacggtgtg tacctgtcaa ctcttcggtc acaagtcata atgttcataa
     1201 acatgaggag ctgttttttg aaattgttcc ttgtgaatca gagttaaacg acaggcttac
     1261 gaactcttga ttaaacggcg ttcaaaagct tgcatacgcg gtaataagaa cagccaggtc
     1321 aaaaacatga gaagccgttt ttcctgggct gttcttatta cttgatgcag ggcagatttt
     1381 tcacaacctc tttattcaga taaaaccaag cttggatcag cattcaactg atagctagca
     1441 aaatttcttt tttgcatttc cacatgtgct gctgctccaa tcattgcagc attatctcca
     1501 caaagtcgaa gagggggaat cactagttct acctcaggga gtttagttgc caaagcatcc
     1561 tgcaatcctt cacgcaaccc ttgattggcc gctacacctc cagctacaat cagttgcttg
     1621 actggataat tttgacaagc tcgaagtgtt ttagtaatca atacatcgat cacacttgct
     1681 tgaaagctag ctgctaaatc atttttgtct agatcttctc cacgctgttg tgcattatgc
     1741 accaaattga taaatgcact ttttaatccg ctaaaactga aatcataatt atcttcatgg
     1801 atcatcgctc gtggaaaatg gtaattatct tttccctgat gagctagctg atcgatttct
     1861 tttccgcttg ggtaagaaag acctaatact cttccaactt tgtcataagc ttctcctgct
     1921 gcgtcatctc ttgtctcccc aatgatttca tatgatccat cttcttgcat atagactaat
     1981 tctgtatgtc ctccgctaac aagaagtgcc atcaatggaa actggaatgg tttaaccaga
     2041 cgggctgcat agatatgccc tgccatatgg tttacaggaa taagcggcaa ttggtgtgcc
     2101 caagcaaaag ccttagctgc agaaatacca atcagcaacg agccgacaag tcctggtcca
     2161 taagtgacag cgactgccga taaatcttct gccgacacac cagcttctac cagtgcatct
     2221 tccaagcata aggtgatttg ttctacgtga tggcggctag ctacttcagg aactacgcca
     2281 ccaaaacgtt tatgactgtt tatttgagaa gcaacgatgt tagataagat ctctgttcca
     2341 tttctaacga ccgctacact cgtttcatca caacttgatt caatcgccag aatcaattct
     2401 tcattcattt catcgtctcc gtttttaact ttctgctcat aatgatcgca tcttccgtcg
     2461 gattgcgata atatgatttc cgtttaccta atatacgaaa cttttctgat tcataaagtt
     2521 ttcttgctgc ttcattagat agacgtactt ctaaaaaaac atgcagcgaa tcttttattt
     2581 gttccgtaat cagtaattgt aacaaatgcc gtgcatgccc ttttccttgt tctgaaactg
     2641 caacagcaat atttgtgatc tcgacttcat ctattacttt cgagtatcca ataaagccac
     2701 agatctggtt atcttcaact aaaagcagat aatcagagtg aggctgttgg atgtctgcca
     2761 aaaactgcat tttcgtccaa ggtgaaccat gtggataagc attttcgcta atcatccata
     2821 cgcattctgc caagtacgct tcatcaaaat ctttttttct gcaaatcaac taatctcact
     2881 cattttctaa aagttttatc atatccaacg catcttcttt tgtttcagtg taatatcttt
     2941 ttttgatcgt tgttgcttga aaacctaact tccgataaag tctttgtgca tcttgattac
     3001 tcatacgcac ttctaatgac atcgtctcac agcgattcat tatagcaaag ttctcgattt
     3061 cgtcaataag cagcgaaccg attcgtttac gctgatatgc cggtcgaacg gcaatattcg
     3121 tgatatggca atctaaaccg aacgctcttg aaccgataaa accaatcatt cttccattat
     3181 caaagatacc tagatataga tgaggaagcg gtgacttcaa ttctgatata aaggcacttt
     3241 ttgtccatgg caattcgcca ttatagactt ctcgttcgac atccagcagc tctttgatat
     3301 cttctgtagt caattctctt agtacgtaac gacgatcgat ttgttcgatt gttttttctg
     3361 tataaatcat tttatcttga aaaaagcttc tggcatacgc tctaaatttt ttcaacataa
     3421 ctttcgtctc caggtgtgtg cgtctccaac catttttctt ctgcttctac ccgttttaaa
     3481 tagcgaggaa gaaaagcttg tttctctgca actggttcag ctaaagcacc taattgagcc
     3541 acagttacac cacttggaat atcccatgct ggaactgtat taatcatcgc actaggcaat
     3601 atttccttta tttcatagaa gaatttgtga caatcactcc cgacgaaata aatagcttca
     3661 ttcagctttt gtagttgtgt gaacaaatca gaagcagaaa tatgtacatc ttcaagtaca
     3721 atttctaatc tattttcttt ccagcggtat gccccagcgt atacattttt tcttcgagca
     3781 tcaaacatgg gcacaataat tttatccaca ccgatgcaat tagccgcgat ggttttcaga
     3841 ctagatactc ctactagctc tttatctaat gtataagcaa tcgtttttgc tgtcgttaca
     3901 cctatacgaa gccctgtata agaaccggga ccatcagaaa ccgcgattcg gtcaatgtct
     3961 tttggactta tttgaagatc tgcaaacaat tggtcgattg caggcatcag tgttacactg
     4021 tggttgcgtt tgatatttgt ttgtatttgt ccaagaattt gttcgtcttc taccacaccg
     4081 atcgccaaag tttggtttgc agtatctatt cctaatgtaa tcatatattt gatcctttct
     4141 gctatgacat tctcgccttc cattgtagca tactttttta ttctgaggat gaaaatacca
     4201 aaggcaaaac tagtacaatg atacatgatt atctgctggc gggcaaattt t
//
LOCUS       DACECZ010000153         4237 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.159, whole genome shotgun sequence.
ACCESSION   DACECZ010000153 DACECZ010000000
VERSION     DACECZ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.159"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="IWO35_002539"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="IWO35_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ0317566.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2945..3379)
                     /locus_tag="IWO35_002540"
     CDS             complement(2945..3379)
                     /locus_tag="IWO35_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317567.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3396..>4237)
                     /locus_tag="IWO35_002541"
     CDS             complement(3396..>4237)
                     /locus_tag="IWO35_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ0317568.1"
                     /translation="SLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKTIEQIKI
                     DNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKN
                     YSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEI
                     GKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNY
                     ISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFN
                     NIS"
BASE COUNT         1295 a          797 c          588 g         1557 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtcctt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa aagtacattt cttcatgttt ttttcggata tttgtgcttt ttaaccattc
     2521 agcagctgaa aacatataaa aagatatgta tgtattcttt ctgaaattaa gttcatttct
     2581 atgcgatgaa gcatgttttc aaaatattac cctttcttag attaaacttc agagtattca
     2641 aataacggaa taaggagcct tatcccgcat tactgaatga gactgtgaca taagtaaaaa
     2701 ggtgacttca aattccttct tcggaatttg aaatcacctt ctttataatt ataaagtatc
     2761 ggtttcacag catttctgaa tgattcctag aatcttaaac gaatgattgg agttatcaac
     2821 attttttcac tcgtttttag cgaaacaaaa aatgacattt gtcacagcct cattaaatta
     2881 ttgattcttt ttctttataa ttgaattgtc catatttaaa gtgtctagta ctctacatga
     2941 ataattaatt gctttcactc aaatacttat aatctattaa cttgtagttg tttaactttt
     3001 ctgaaaaagt tagacccacg taataatctt cccagctttc tctttcaatt aactgatcca
     3061 atttttgata atccataaat tcatcatgta tcaaaaagaa taaataactt gatattttag
     3121 catctagctt aatcagatta aataatgttt gtttaaaatt gctaccatta atatgactga
     3181 tagattctct agcttcttca cataaacttt ttactttatt aattactact ttgtcatatt
     3241 catttaacac actattatta ccattactta gacgctcaga ttctacgaaa caactcaaat
     3301 cagaaatttc ccaaaaaaat tttttgttaa aatccaattt cctcaactcc tccaaactac
     3361 gtaaatgata tagtctcata ataaaataaa acctctcagc taatgttgtt gaaattgtcc
     3421 actaaaccta attgatattg cttaggagat acacctgcgt actttttgaa tactctcgaa
     3481 aagtgtgaag aatctgaaaa tcctaaacct aaagaaactt cactgatagt atggtttgat
     3541 ttcaacatta ttttagccgt agaaattttt cgcttagaga tataattata aatcgtaata
     3601 tgcatttcat tcttaaagct ttctcttaat ttagtttcac ttacagaaaa ataattagct
     3661 atttccttta cagtaattct tttatagata tttaagccga tgaattgaac gacagaagaa
     3721 attaagggag aaagatgctc tatcttagtt tttccgattt ccttagtata ataaacaatc
     3781 ccagaatccc tcacttttaa gacctcaggt aacgatacag ctaattcgtt ttcttgtatc
     3841 aaagcatctc tactttgata cgcttctatt atatccattc ctaatgttat agacgtttgt
     3901 gctaactttt caaaaacaat gatagaataa tttttttcag accgaatcgt atctccacta
     3961 acacttggaa ttattccatt actcagatta aaaaccatgt tttctaaatt tcgaatatct
     4021 ccatttttaa catattccag tattttattt tcataaagaa acagatatat ttctggatcg
     4081 taattttgtg ataacagatt atctattttg atttgctcga tagttttact tagattttct
     4141 gtatattcaa ttaacggttc atggaataga tcttctattt ttccagtaaa aaaataatga
     4201 actaaaatca gaatatctcg aatgtctcct aaagaat
//
LOCUS       DACECZ010000154         4211 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.160, whole genome shotgun sequence.
ACCESSION   DACECZ010000154 DACECZ010000000
VERSION     DACECZ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.160"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(138..380)
                     /locus_tag="IWO35_002542"
     CDS             complement(138..380)
                     /locus_tag="IWO35_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0317569.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(412..969)
                     /locus_tag="IWO35_002543"
     CDS             complement(412..969)
                     /locus_tag="IWO35_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0317570.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(982..1170)
                     /locus_tag="IWO35_002544"
     CDS             complement(982..1170)
                     /locus_tag="IWO35_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0317571.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(1183..1749)
                     /gene="amaP"
                     /locus_tag="IWO35_002545"
     CDS             complement(1183..1749)
                     /gene="amaP"
                     /locus_tag="IWO35_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0317572.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            2979..3452
                     /locus_tag="IWO35_002546"
     CDS             2979..3452
                     /locus_tag="IWO35_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ0317573.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            3461..3688
                     /locus_tag="IWO35_002547"
     CDS             3461..3688
                     /locus_tag="IWO35_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0317574.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         1273 a          752 c          630 g         1556 t
ORIGIN      
        1 ttttttattt ttaaagatat tttttcataa ggaataaaga atacttcata taaaatatat
       61 aaaaaagaac cgcagcgagt gtcgctacgg ttctcaaatt tgttcttcat ttcaacataa
      121 gtttttcttc aatttgctta taatttacgt gcaaagaatg aaacgactaa tactaaaatg
      181 attgcaccga tcaatgatgg aatgatagcc attcctgcaa ttgttggtcc ccaagttcct
      241 agtaaagctt gtccaattgc tgaacctact aaaccagcaa tgatgtttgc gatgatcccc
      301 attgatgatc ctttattcgt gattgctcct gcaattgctc caatgatacc gccgacgatt
      361 aatgcccaaa taaatcccat tttaaattcc tcctaactga cttcttactt cttattgtac
      421 acgtggttcg ttattttctt tgactttttg tgcacctttg cctacagcag attttgcttt
      481 atctgtttgg cgtgaagcaa attgtcctgt ggattttgct gcttctgtta ctttgtcttg
      541 tactgtttct tgatcttttt ggtattcttc ttgtgttttg atgtctgcaa cattcacatt
      601 cacttcgata acatctaaat gtgtcatatt gtttacttca gtactgatca attctttgat
      661 ttggttgtaa atatcttcga tgtctttacc gtattctacg atcacatcta gatccacagc
      721 tacttgtttc ttacctactt ctgtattgat acctgctgtt acgttatctg tgttgactaa
      781 tttttcagcc atatttgaga aaaatccgcc atctaatgtc aatagaccat ctacatcttt
      841 caatgcataa ccgatgattt tttggatgac tttgtcttca taagtcaatt caccattcat
      901 catgcggttt tctccttttt catttgccaa aactgatcca tttgttgtat tcatgttttt
      961 gttttccatc tttgtttcct cctatgattt aaaaaattga tcaactattc ctgactgttt
     1021 caaataaaaa ccgattcctg cgccaattgc tgtcatgatt atgatcaaca atgtcttgaa
     1081 aaatccaatc gttaaccata aaacagctag cagtagtcca agacccccaa agataatagg
     1141 gagcttgtat gctgaaaata gttctttcat tatttggccc tcctattcca ctcgagagtg
     1201 cttgtagtct cttgtatctt cttgttcttc ataaccgctg tatgtgactg ccactttgac
     1261 cgtttctttt gttccgagta ctttagttac ttcttcttcg atatcttgca tcaatacacc
     1321 tgttttccct actaatgaag aagttcgttt caagtctccg ttgacatgga tatggattcg
     1381 atttttcgtt gaacgtatcc gtactttagg tgaatgaatg aactcttctt cgtgtaaatg
     1441 ggaacgtacc aacccttcga tcgctttttt gtcgattgtt agtttgccat cttcccgttt
     1501 cataacgaaa gttcctcttg actttggata gaaaagaacg actagcaaga tgataatagc
     1561 aagaacggca aatactacag ctgcccagaa aatatattgt tgcatgtagt aattcgttaa
     1621 agtaaagaag cgaaatcgtt ccaattgttc aggcataata tacggcgcat tgatcaacgc
     1681 aatcgctaga aatactgaaa gtagcagtaa actcatgata acaccgatta tttttaaccc
     1741 cttattcatt ttccacctcc tataaactta tttttaccct gtaaataact ctaccgacaa
     1801 tcacagtata gatagcgaac agttagcata gcatcagtaa ataaagcgct accaaacatt
     1861 gctggatcat aacggcgttt tattgccagt ctgatactga aataacaatt gcttacttat
     1921 tctctattta cttttgattg ctggctatac ttaaaggata ttattcaaga ttaaaaattg
     1981 caaagattat gctcagttat tattacaaga ttctatttat ttattaaatt ttttcggaga
     2041 attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt atttttatat
     2101 tatcattttt cttattcact tctttggttt atactattct tataaataaa aacgaggtat
     2161 gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat ttgattgtat
     2221 caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact actacattat
     2281 gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga aaaaaataaa
     2341 catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt attcggcaat
     2401 ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat aaactaatcc
     2461 aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc catgcgtcaa
     2521 tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata agtggtgatt
     2581 acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac ttgaaaaaat
     2641 taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct gttaacttac
     2701 acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat aatcccaggc
     2761 atctaaacta gcattataga agagacacac atagaatcta aaataactgg ttttaaatgt
     2821 ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct atcatttttt
     2881 ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa taattattct
     2941 gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa acattgctat
     3001 taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta cttttttata
     3061 ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca ttatttgttt
     3121 ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta ttgaaactat
     3181 tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata ctaaaaaaaa
     3241 tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct aaatttatga
     3301 aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct gtcgtattca
     3361 ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa catgcgattt
     3421 tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta tcgttaattt
     3481 agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga aagtgggcat
     3541 tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc gtttttccac
     3601 tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga cctataaaaa
     3661 tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa ataaaaaata
     3721 atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat tcagattttt
     3781 tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca tacaaagggg
     3841 gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct gttacgttgt
     3901 agttggatct gcttttagta taatacttta ttcattccac cagactgata gcagtttgag
     3961 cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga agtgcttaaa
     4021 acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt aagctcccat
     4081 atatactgta aagccttcta tatattatgt catgttgctt tttaacattt actatgtagt
     4141 tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt aacattattt
     4201 tgataataat g
//
LOCUS       DACECZ010000155         4198 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.161, whole genome shotgun sequence.
ACCESSION   DACECZ010000155 DACECZ010000000
VERSION     DACECZ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.161"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            127..579
                     /locus_tag="IWO35_002548"
     CDS             127..579
                     /locus_tag="IWO35_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317575.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="IWO35_002549"
     CDS             593..1282
                     /locus_tag="IWO35_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317576.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="IWO35_002550"
     CDS             1407..3758
                     /locus_tag="IWO35_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ0317577.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..>4198
                     /locus_tag="IWO35_002551"
     CDS             3838..>4198
                     /locus_tag="IWO35_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317578.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFN"
BASE COUNT         1458 a          539 c          846 g         1355 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gtttggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaacc
//
LOCUS       DACECZ010000156         4184 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.162, whole genome shotgun sequence.
ACCESSION   DACECZ010000156 DACECZ010000000
VERSION     DACECZ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.162"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            109..615
                     /locus_tag="IWO35_002552"
     CDS             109..615
                     /locus_tag="IWO35_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ0317579.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            632..1531
                     /locus_tag="IWO35_002553"
     CDS             632..1531
                     /locus_tag="IWO35_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ0317580.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1618..2502
                     /locus_tag="IWO35_002554"
     CDS             1618..2502
                     /locus_tag="IWO35_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0317581.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2694..2765)
                     /locus_tag="IWO35_002555"
     tRNA            complement(2694..2765)
                     /locus_tag="IWO35_002555"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2730..2732),aa:Arg,seq:ccg)
     gene            2825..4159
                     /gene="rpoN"
                     /locus_tag="IWO35_002556"
     CDS             2825..4159
                     /gene="rpoN"
                     /locus_tag="IWO35_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ0317582.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
BASE COUNT         1518 a          762 c          843 g         1061 t
ORIGIN      
        1 cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa attgaaactt
       61 ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat gatttttact
      121 ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg actcatcgaa
      181 actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa agaagaggcc
      241 ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa agaaaaagag
      301 atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt agatagttgg
      361 cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc ccaacagtta
      421 cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga cagtccggaa
      481 aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat tgcgactatc
      541 ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa tgctttacgt
      601 caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa atttaaaaaa
      661 actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg agatccgcca
      721 gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga gacgtctcca
      781 agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc tttttaatag
      841 tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg aattgatctt
      901 aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga agatcaatac
      961 acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact ttcgcgaagg
     1021 acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg atcattacca
     1081 agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga ttgaaaggca
     1141 gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa tcgacaagaa
     1201 aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc tatctgtttt
     1261 gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat ctaagtacta
     1321 ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg ttacgcctac
     1381 acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc aatacgaaat
     1441 tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga caaaaacgat
     1501 tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact agcagtttca
     1561 attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag agatatagtg
     1621 aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt tttcgaaatt
     1681 acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc ggagcattcg
     1741 tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac tgaaacagct
     1801 aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc atcacaatca
     1861 acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc aatcagcttt
     1921 gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa agaaaaaaac
     1981 gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga aatcgtgaag
     2041 cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca gaatctggcc
     2101 actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga gatcaaaaaa
     2161 gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac gaatgtagga
     2221 gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga ttggaagaca
     2281 agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg ggcaattatt
     2341 ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat cgattatctg
     2401 aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc aacggctgtc
     2461 gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca agtatcttta
     2521 ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc aaaggcttaa
     2581 caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg gacagttttc
     2641 acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta gaacgcgtcc
     2701 tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca ctacactaag
     2761 gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg gattggacaa
     2821 aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga aactagccat
     2881 gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg agctgttggc
     2941 attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc cggaatggca
     3001 gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa attacctaaa
     3061 tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag tccatttgaa
     3121 ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca tcgatttgaa
     3181 cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta cgcctatcca
     3241 aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag gtgcacgtag
     3301 tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg aattagctta
     3361 tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag aaatcgcaca
     3421 acgtttttct gttgatctac atgctgttca gcagatattc gactatttac aaacgttgag
     3481 tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc ctgatgtccg
     3541 ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa atcagccaaa
     3601 tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata aagaaactga
     3661 aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga tcctacaacg
     3721 tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag ctttctttat
     3781 cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg agatagatgt
     3841 gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag acttcggtgt
     3901 atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag aacagacaga
     3961 agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc aagaagacaa
     4021 aaacaagccg ttgtctgacc agaaattggt tgaattgctg aaaaaagatg agatagcgat
     4081 ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt catcaaaaag
     4141 aaaacgttat gataactaat cagtcaaaag actagaaaaa gtat
//
LOCUS       DACECZ010000157         4109 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.163, whole genome shotgun sequence.
ACCESSION   DACECZ010000157 DACECZ010000000
VERSION     DACECZ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.163"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            54..1355
                     /locus_tag="IWO35_002557"
     CDS             54..1355
                     /locus_tag="IWO35_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0317583.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1382..1642
                     /locus_tag="IWO35_002558"
     CDS             1382..1642
                     /locus_tag="IWO35_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0317584.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2641..3876)
                     /locus_tag="IWO35_002559"
     CDS             complement(2641..3876)
                     /locus_tag="IWO35_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ0317585.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1168 a          665 c          862 g         1414 t
ORIGIN      
        1 ttgcgttgtc atgcaatgaa tggttcatat tttattgaaa aggagtggtt ttagtggaaa
       61 agctgatgaa atgggtcgaa gagaaacttg tcccaccaat ggcaaaaatt ggtacacagc
      121 gtcatttatt agcaattcgt aatggggttg tttcaacatt gtcattgatt ttgattggga
      181 cattttttat ggtctttatc aatttaccgt ttcctggatg gaatgaattt attgcccctt
      241 attcggcaac gattgtttta ccctttagga tgacgatggg attgatggct atttatgcaa
      301 cttttgttat gggttcggat ctagctaaat catatggatt agatagtgtg acaggtggta
      361 tattatcttt aggaacgttc tttatgttac aagttcctgt aaacgtttta actcctgaag
      421 aggcacctct tggctgggtc ttacctatga gttctttggg agcatctggg atgtttgcgg
      481 ggatcttatc tatgattttt gcagtggaag tatatcgttt ctttaaaaat cgaaatatta
      541 ctatcaaaat gccagaacaa gtgccacctg ctgttgcccg tagttttgag gcattgattc
      601 caggtgcagt cattttaaca attacttggc tgattcgaag cgtcgcgggc tttgatgtaa
      661 acgctgcctt attatcgtta ttcgagcctt taaccaatat tttagggaat aatttgcttg
      721 gtgttttact accaatgttt ttgatccatt tgttgtggtc atttggtatc catggcatga
      781 gtattgtagg ggctgttgtt cgtccaatgt ggctgatcat gttggatgag aatgcaaagg
      841 cattggcaga tggtacagct gccacccaat taccctatat cgctcctgaa cagttttatc
      901 aatggacggt aacaattggt ggtgcaggag caacgatcgt ggtatctgtt ttgtttatat
      961 tcttctgtaa atcaaaattc ttgaaagagg ttggtcgttt ttctattatt cctggtatct
     1021 tcaatatcaa tgagcccata atttttggag ctcctatgat gttgaatcct tacatgttca
     1081 tcccgttcaa cttagttcct ttagttttga ctattatcag ttattttgca gtcaaattag
     1141 aaatggtcaa tagatttact gttttgccaa catggacatt accagctcca atcggcggtt
     1201 atctttcagc aggaaatgac tggcgtgttg ttatcttgat catgatcaac acattgatag
     1261 catttattat ctattatcca tttgtaaaag cttatgataa aaagatgctg tcagatgaac
     1321 aaagtcgtgg agaaaacgaa gtccagactg tctaagtttg gtttttagaa ggtgggaaac
     1381 aatgtattgg ctgattttat tttttgtttt tatcttttta ttaacggcta gtcaccttat
     1441 attgaatatg ctagctacat atcatattca aatcaatcga tggatttggg cactggcatc
     1501 atttttgatc gtgattcttc caaagatcat tgtaccgcat atgaatgttt tgttttcatg
     1561 ggggacatat gtactttgtg gaatttttgc tatcaatttt atgatcgagc agcatagatg
     1621 gtttgtcaca tcgaagcttt aaatgttcta aaagtggttg taacagaagt ggttagcttc
     1681 aagaaataag aagaaattta cgaaaattgc ttcttaaatt tttgtgaaat gttggcttat
     1741 ttttcgtttg gttgaactta ttaccgcaga tgcaagcttt tgaccaccgt ttattcggaa
     1801 tatgggactg tgacaaggct tttgtcacag ccctttttta cataaagatt attattgttt
     1861 taactgtttt ggcagaaaga aggaggaaaa aatggaaatt ttatcagcaa tggatttaga
     1921 attagtagat ctactaccaa agtgatcaag tcaaatatag atttgggagc ttacaaccat
     1981 atagtagggg ctgcagtatg gtgtatgaat ggtaatatgt atgatgtgag tgtttattca
     2041 aatcatgaaa cgtgtataga aataattgtg ctgggaatag caatcagtca tggtgaacgt
     2101 gacttttagt gtatcgttgc tgacgacata gaacattctg acatgatcta ctcaatatgt
     2161 ggggattgct ggcagtttat ataggagtat gcatcggatt gcgagatcat tgtctcaaca
     2221 gaagagggag gttgattatc aaagataaag tggtaaatcc tacatttgat ttagtaattg
     2281 ttgaggggac tagcggcaca aatctctaca aagcgatgct tctagatcta tatgacacat
     2341 tttaagaaaa tgtatttgcc taattattat ggtctttctt ttgaagaaac gttgagatga
     2401 tccaaccaga gaaaggtgtc aaagaatttg actaagaata aatggcagac gctacctatg
     2461 ctcatttgga tagcgtcaga tcttttattt taaggatgat tttgtgtttt ttagacaccg
     2521 taatttaagt aactctaact tttcaatcca ctatttactt tttttgtatt ttcactgtct
     2581 gggtctcgaa tagcgaaatt actgatgagt ctctattaaa aaagcttttt ctacttgaaa
     2641 ctatattggg tggttgctca ggaaggtttc gatttcttga attgcttgtt gtggaccatt
     2701 tattggaaaa acggcttgca gcgtgcgtgc ctgctgacga atcttgggtg aagaaacttc
     2761 gcgcagagca atctgcagtt ttctttcagt cactggtttt gtgagggcga ttgcgtttcc
     2821 gtattttaca caatagtcaa tattacagct ttgctcaaat tgcatcccca caccgataaa
     2881 cggtttgccg gattcacagg cagtttggac ggtcccttcc ccgccgtgga taaaagaaaa
     2941 atcaatgaat tcatgcaaaa gatgactaga cagatattct gttattaaaa gatcttctga
     3001 gtcagattga atgtcatcaa gcagatagtc aataggtgca ataaccgcta gatccggttg
     3061 tttttgtaag aagaacaaaa ttttgcgtaa catacgcgga ttgcctgaac tacccattgc
     3121 gaaatagata atacgttttc tttcttgttg ctttcgctgt ataaaagtta gcacttcttc
     3181 aggcaaggaa gtactgagct tagcaaaaat tggaccaacg attcgataat tttccggcag
     3241 ctgttcttct tccaaaaata gtttaggcgt tgtgatgaga tttagatcag catccatcat
     3301 ttgtaaactt gtttttggta aaggaagcat ttctttttgt gcagcgtgtt tgaaggcatt
     3361 ggggatccat cgggtattta ctatacgctt ttttgtccag tgccaaaggg gattgcttaa
     3421 agggccgaag aaacgtaact cgtggggcat cagcgcatgc tctgattgaa aaaaaggttc
     3481 actcaaagca tagggtttaa catatactaa aggaatcttt ttattgcggg ccgataaaaa
     3541 aattgtcaca ttcgaacctg tcacaatcag tcgaggctca aacttagcaa tcagactctg
     3601 ttcactagtg atacgagagg caaccatttc tttggtaaaa gggtttttaa gtgtacgcaa
     3661 ctggtcaaat tttatgattt gttcttcttg ttttttcgta aggatcggcg ctagtaaatg
     3721 atactcaaag ccactttctg tgatcaaagg agcaaaagtt ttagaaaagc caaagaatac
     3781 acagtaataa cggtctttca tagctttggc aacctcaatc attcgtgttg tctcagctaa
     3841 attgaatact gcaggagcaa aaagaattgt cttcatgaaa tcctccttat tggaatgagt
     3901 ctatcccttc gtttcttact agtatactaa aaaacacagc gttcgagtag cctatgtata
     3961 ttttgaatat taaactgtct ctcgctgttt tcacaacttc taaatttaaa ttttgtaatc
     4021 aagttgaaac tccaattttc aaaaaaacac gcaacaattt tttgatcgat ttatatgttg
     4081 tgccagataa atacgagaag ttgtgtcat
//
LOCUS       DACECZ010000158         4077 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.164, whole genome shotgun sequence.
ACCESSION   DACECZ010000158 DACECZ010000000
VERSION     DACECZ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.164"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(61..2424)
                     /locus_tag="IWO35_002560"
     CDS             complement(61..2424)
                     /locus_tag="IWO35_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAQ0317586.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2741..3928
                     /locus_tag="IWO35_002561"
     CDS             2741..3928
                     /locus_tag="IWO35_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ0317587.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT         1158 a          806 c          796 g         1317 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgattacgt
     2641 ccatataatt gtgtaaaagt gaagcatcaa aaaagccatt gatctcagtt aggagtacaa
     2701 tgttttttgc agaaaacata ctcgaatagg actgatatca atgactcaag tacattttac
     2761 ttttgaaagc gaagaaattc aagcaattat aaacgaaagc ggtgcgaatg ataccgcaaa
     2821 aacacttatg actatcatgt ttaatcagct gatggaagaa caacgaaatc aatatattca
     2881 ggcaaatgcc tatgaacgtt cagaagaacg gcaatcacaa agaaatggtt actacgatcg
     2941 ttcgtttaca actcgaattg gtacgttaga acttcatgtt ccacgaacca gagacggtaa
     3001 gtttagtcct actatctttg aaagatatca gcgaagcgaa aaagctttga tcgctgccat
     3061 gattgaaatg gtcatttctg gtgtctccac tcgcaaagta actaaaacag ttgaactgct
     3121 aacggatggt gcgactgttt ctaaatcatt cgtttcaaat ctgatgaaac agttggatcc
     3181 atttgttttt gagtggagaa accggagcct agaaggctcg gaatatccat ttttcatgtg
     3241 tgatgccctg tatatgaaag taagggaaaa tcatcgtatt gtctcaaaag gtgtatatat
     3301 tggtatcggc attgattctg atggacgacg tacgattctt ggttttgatg ttcaagacgg
     3361 cgaatcggaa gataattggg ataccgtttt tcaatcgttc gttcaacgag gtttgttcgg
     3421 tgtaaaactg gtcatttctg atgcacacaa agggttagtt aaggctgttc gcaagaactt
     3481 tttaggtgcg agctggcaaa gatgccaagc ccactttttg agaaatattt ttgataagct
     3541 gccaaagaaa gtttcttctg atgtaaaaga cgagttgaag agtatcttta aagcctctga
     3601 acttgaattg acacgtgaaa gaaaagagca cttcttggaa aaatatggtt gtgattcaaa
     3661 attaagtgct gcttgcgaca ttttagaaaa cggctttgaa gatgctattc aaattttgtc
     3721 ttttcctgaa aatatccgcc gaaggattcg tacaaccaat gtgttggaac ggttgaacga
     3781 agagattcgc cggagagaac gagtgattcg gatttttcca aatatcaatt ccatcactcg
     3841 gataattgga acacttttga tggaaaaaga cactgagtgg ctggcttctc cacgaaaata
     3901 tttagaattt aattcgaata acatttagca ccatgaacct gtgcgagtct cttctttctg
     3961 aggagagaca tcaagagtcg ctgcgctact gctcctgacc tctctcctca gaaagaaagg
     4021 catggcagtt aggttcacaa cgcaaaacat tgtgcttttg atgaggttga actttta
//
LOCUS       DACECZ010000159         4041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.165, whole genome shotgun sequence.
ACCESSION   DACECZ010000159 DACECZ010000000
VERSION     DACECZ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.165"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            179..775
                     /locus_tag="IWO35_002562"
     CDS             179..775
                     /locus_tag="IWO35_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0317588.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IWO35_002563"
     CDS             899..2698
                     /locus_tag="IWO35_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0317589.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="IWO35_002564"
     CDS             complement(2976..3188)
                     /locus_tag="IWO35_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317590.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT         1188 a          688 c          712 g         1453 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctataatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaatat a
//
LOCUS       DACECZ010000160         3910 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.166, whole genome shotgun sequence.
ACCESSION   DACECZ010000160 DACECZ010000000
VERSION     DACECZ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.166"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            86..1507
                     /gene="gndA"
                     /locus_tag="IWO35_002565"
     CDS             86..1507
                     /gene="gndA"
                     /locus_tag="IWO35_002565"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ0317591.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            1700..2386
                     /locus_tag="IWO35_002566"
     CDS             1700..2386
                     /locus_tag="IWO35_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0317592.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            2383..3888
                     /locus_tag="IWO35_002567"
     CDS             2383..3888
                     /locus_tag="IWO35_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0317593.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
BASE COUNT         1318 a          676 c          837 g         1079 t
ORIGIN      
        1 atgttgttct ttagtttgca atgtagccgt gttaagctat aatgtaagag attagggaga
       61 aatcccgtat gtaggaggag ttcatatgtc aaaacaacaa atcggcgttg tcggtatggc
      121 cgttatgggt aaaaatctag ccttgaacat agaaagccgc ggctattcag tggctttatt
      181 caatcgtaca ggggctaaaa caaccgctgt agtagaagaa cacccagata aaaatttcaa
      241 agcaacatat acaatcgaag aatttgtaga atctatcgag aaaccacgtc ggatcttgct
      301 aatggtcaaa gcaggaccgg caacagatgc aacaatccaa gagttacttc ctcaccttga
      361 taaaggcgat attttgattg acggaggaaa tacattcttt aaagatacga tgcgtcgtaa
      421 tgaagagtta gccaactctg gcatcaactt tatcggtaca ggcgtttccg gtggtgaaga
      481 aggagcttta aaaggacctt caatcatgcc tggtggacaa aaagaagcct atgcgctagt
      541 tgcaccaatc ttagagcaaa tttctgcaaa agcagaagac ggagcaccat gtgtcacata
      601 tattggccca aatggagccg gacattatgt gaaaatggta cacaacggta ttgagtatgg
      661 agatatgcaa ctgattgctg aatcttatga cttaatgaag aatattttaa acctttcagt
      721 agaagaaatg gctgatatct ttaaagaatg gaatcaaggc gaattagaca gctatttgat
      781 agaaatcact gctgacattc tgacacgtaa agacgatgaa ggaacaggta aaccagtagt
      841 agatgtgatc cttgatgcag ctgggaataa aggaacaggt aaatggacaa gccaaagcgc
      901 gcttgactta ggtgtgccac ttccattgat cactgaatct gtatttgccc gctatatttc
      961 tgcttataaa gaagaacgcg tacaagcaag caagattttg tctcgtacga acgattttga
     1021 gttcacggga gacaaaaaag aattggtcga aaaaatccga gaagcgttgt atttcagtaa
     1081 aatcatgagt tatgcccaag gatttgctca attacgtgtc gcttcaaaag aatttgattg
     1141 ggacttgcca tttggtgaaa tcgcaaaaat ctggcgcgct ggatgtatca tccgagctcg
     1201 attcttgcaa aaaattacgg atgcctatga caaaaatcct gagatcgaaa atttattgct
     1261 agatgattac ttcgttgaaa tcacgaaaaa atatcagcaa tcagtcagag acgtcgttgc
     1321 cttggctgtc caagctggcg tacctgtacc gactttctca tcagccatcg cttacttcga
     1381 ttcatacaga gcagaacgtc tgcctgcaaa tataatccaa gcccaacgcg attactttgg
     1441 cgcacatacc tatgagcgtg tcgacaaaga aggaatcttc cattactcat ggtaccatga
     1501 agaataatag tctttcggct gttaggaaat gagaaaataa aaagaggacg aaagagctga
     1561 agtcgattgt tatgagtaga cttcagctct tttttaaaat tgctatccat gataaaatga
     1621 ctatagattt ttaagcatat tatcggtata atgatagagt ttttaaaaag ccagtagaat
     1681 agaaaggata aaataataaa tgagcaatat tctaatcatt gaagatgaaa agaacttggc
     1741 acgatttgtc gagctagagt tgaagcatga agggtataac gcagaagtcc actacaacgg
     1801 ccgaactggt ttagatgcgg cattaaataa tgagtgggat gccatattgt tggatctgat
     1861 gctcccagaa ttgaacggtt tagaagtctg cagacgtgta cgccaagtca aaaatacacc
     1921 aatcattatg atgactgcaa gagattctgt gatcgatcgt gtatctggac ttgaccacgg
     1981 agcagatgac tacatcgtca aaccatttgc tatcgaagaa ttattagcac gtcttcgtgc
     2041 attgttgcgt cgaatcgata ttgaaggcga taaaaatgtg gctaaacaaa ctactattac
     2101 ttatcgcgat ttgactatcg aaaaagaaaa ccgtgtcgta cgtcgcggaa atgaagtaat
     2161 tgaactaaca aaaagagaat acgagttgct gcttacttta atggaaaacg tgaacgtggt
     2221 attagcacgt gacgttttac taaataaagt gtggggatac gaaacagaag ttgaaacaaa
     2281 cgtagtggac gtatatattc gttatcttcg caacaaaatt gacgttcccg gggaggaaag
     2341 ctatatccaa actgtacgcg gaacaggtta tgtgatgcgc tcgtgataaa gccgaaaaaa
     2401 tcggaaaaaa gagaactgaa agggccctcc ttgacaatca agtgggcctt tgcgagttct
     2461 ttcttcatat tcgttgtgtt tacaattttc gctgtcatta cctataaatc atctatcaat
     2521 ctgattgtgg caaaagaaag aaacaatgta gagcgtacga tttcagaagt agcttctcgt
     2581 ttggctaact cggatgaaga actgacatta gtaaatacgt atcgtaatct gacgaaagcg
     2641 acaaatgacg gagatgaggt cgtagatcat acttcactgg tcgaaggaaa cttaatgaag
     2701 atcgattcct ttatagctga gctagggcag ccatcaatgg atctttatgt ttatgatttg
     2761 gacgagaact tgatttttaa aacccatgaa aacagtcgaa acctgattca gactacacgt
     2821 aaagttccaa cgatcgttac actagaagga aaaactggct ttttatctat ccagcctata
     2881 tattctaaag gtacaagaga aaagatcggc tatgcgcaaa gcttctatga attatcttct
     2941 ttctatgata ttcgaaataa gctattattg actcttattg ttttagaagt tgtttctttg
     3001 atcttaagta gtattttagg tttcttttta tcttcttatt tcttaaaacc actcaaggta
     3061 ctcagagata caatggatac tatacgtaaa gatccgcaat cagatatcca catgcctgaa
     3121 atcgacacaa atgatgaatt ggcagattta gctgaaatat tcaatgagat gcttgaccgt
     3181 atgcgtttgt atattgaaca acaagagcaa ttcgtggaag acgtatctca tgagttaagg
     3241 acacctgttg caattattga agggcatctt agtctgctaa atcgttgggg gaaagatgat
     3301 cctgagatat tagaagaatc attaagcgcc agcatgcaag aaatcagccg tatgaaaagc
     3361 ttagttcaag agatgctaga cttatcaaga gcagaacaag tagatgttca ttatgcgaat
     3421 gaaacaacta aaggaaaaga agtcacctat caagtttata ataatttcaa gatattatat
     3481 ccagactatg tgatcacttt agatgatgat ttgacaaaag aagtgaccct gcaaatttat
     3541 cgtaatcact ttgaacaatt aatcattatc atattggata acgcagtaaa gtattcaact
     3601 acacgaaaag aagtccatat ctctatttct tccactctga atgaatttga gatcgcgatt
     3661 caggactttg gcgaaggaat acccaaagaa gaccttaata agatcttcaa tcgtttttat
     3721 cgagtggata aagcaagagc tagaacgaag ggtggaaatg gtctggggtt gtctatcgct
     3781 aaacaactgg ttgaaaacta taaaggacgt atcttagcag aaagtgtagt agggcaaggg
     3841 acaattttta gaatctttat tcctatcgca aaggcagaag acaagtaaag catgtgcttt
     3901 acttgttttt
//
LOCUS       DACECZ010000161         3883 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.167, whole genome shotgun sequence.
ACCESSION   DACECZ010000161 DACECZ010000000
VERSION     DACECZ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.167"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..417)
                     /locus_tag="IWO35_002568"
     CDS             complement(<1..417)
                     /locus_tag="IWO35_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0317594.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQF"
     gene            1109..1267
                     /locus_tag="IWO35_002569"
     CDS             1109..1267
                     /locus_tag="IWO35_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0317595.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            1339..2565
                     /locus_tag="IWO35_002570"
     CDS             1339..2565
                     /locus_tag="IWO35_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0317596.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(2653..3045)
                     /gene="rpsI"
                     /locus_tag="IWO35_002571"
     CDS             complement(2653..3045)
                     /gene="rpsI"
                     /locus_tag="IWO35_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0317597.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(3061..3504)
                     /gene="rplM"
                     /locus_tag="IWO35_002572"
     CDS             complement(3061..3504)
                     /gene="rplM"
                     /locus_tag="IWO35_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ0317598.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
BASE COUNT         1328 a          654 c          638 g         1263 t
ORIGIN      
        1 aaattgtcct ccagataatt ctcctatttg atgattagaa tattttccca tatcgacctt
       61 ttccaaagcc tttgaaactc tttgccattc tgcattttta attctttgaa aaactttcat
      121 tccggcatat gttcccatag atacacattc ttttactttt attggaaaag taaaatcaat
      181 atcagctttt tgctctacat atgcaatctt caccagtttt ttattgatat cttcattatt
      241 aatggagacc tctccctgat gagggataat atttaacatt gctttaagca atgtggattt
      301 tccagcacca tttggaccaa taatacctgt taaagcacct tcttgtattt ttaaagaaat
      361 ctcgttcaat gctaaaaaat cgttataaca aacattaagc tttttcactt ctaacataac
      421 tttctccttc taaatgataa tgattatcac gatcatatgg tatacaaaaa ttatatttta
      481 gtcaacccta aattttatag tttagatttt tttctgttgc tgaagattag aatgacaaaa
      541 ctatttgaaa ctacgcgtat agtaagtagc tttcaaaata tataaaaaag gagcgttctc
      601 aaatgagaac atgctcctaa aactacttta ttggcgtatg gaaaaattat acttgaagtg
      661 gatagcgggt tgaataagtt gataagcatc aaaacattta taaaaattat tcaagactat
      721 gtctgttcca tatacaaata tgaaaatcag taaactccat ttaacttttt tcgtagtgtg
      781 tctttttata catgtttatc gttattaata gtgaatagga aaaaaactat ttttgatcat
      841 tgacaattaa atacaatttt tgaataacga tccctgctat tcgagctaga aactcaagta
      901 cgccatgacc tgattagcga caaatactta gagatttctt tttgttctgg gaaaacaaaa
      961 tgccatgaca atagcagcaa ataatgatac ttccgtaagg ctcgccgtaa actggggaga
     1021 ggtgaaattc ctatgtgctt acccaaagca attcaaaaat ttcataacta ctattttagg
     1081 agatgatttt atagaatcca gagtctacct gaataaaaaa caaacctgtg catttctagg
     1141 aatcgataat aatactctag ataagtggat tcgtatatat aatttcccag taataagagt
     1201 tggtggtgta gttcgctttg acgtagaaga tattaaagac tttatgaata catttaaaat
     1261 aacttgaaat tttcttctcg ccgcatatac taaaattagt gaaatatact gccttacttt
     1321 ttttaaggag tgaggatttt gcctacaaag ctttcaaatg ggaaatataa aactaatctg
     1381 agatatccaa aaaggtttaa agaaataaca ggaattgcat cagaaaagta tcaaaaaaca
     1441 tttccaaata ggcaattggc gattaaagcc gaaaatgata tgaagaagaa aatagaaaaa
     1501 gttttgagag aggagaatgc aaactcatta gaattaaaag gaaaaataac gtttaaaaaa
     1561 ttttacgaat ctaaatggct tcctagatat gaattaggtc aaacaattag atcaaacagg
     1621 cctcctagtg atataaccat tagtaataca aaagatatat ttcggttgca tattttacct
     1681 atgtttggtg aatacgcaat gaattatcta aatatcaata ctgagataat atcagatgaa
     1741 ttaaccaaaa aatcgaaaga atatgcaaat ataaaaatca taaaaggata tgttcgttcc
     1801 atgttcgata ttgcagaaat actaaattat atcgaattta atcgaactac taaaattatt
     1861 caaagtatca ctgcacctaa aaaaaatgct ttagaagaaa aaagaataca agaaggtaag
     1921 caagcactat catcaaaaga acttactaat tggatagaag cagtaaatga tgattttaat
     1981 aatcatttac ttacttttca tgattacaca ctttttatga ttaccctgta tcttggtgat
     2041 agaaaaagtg agacttacgc tttgcaatgg aaatatattg attttgaaaa acaaacggtc
     2101 cgtcttaaac atactttaga taaatatcaa agaaaaaagt ttacaaaagg tcgaaaagat
     2161 accgtcattc aagtaccaga agttgtaatg acattgctaa gtgaatggaa atcggtacaa
     2221 gctgatcaat tgttgaaatt aaaaattaag caaacgttag atcagtatct ctttacttat
     2281 actaaaccat ctggagaagt aaattgtcct gttcatgccg attacctaaa ctatcgaatc
     2341 aattcaatta aaagaaggca tcctgactta gttcatttat ctccacacaa actccgtcat
     2401 acttatgcaa ctatagctag acaaggtgga gcagatatga atcaaatttc aaatgcttta
     2461 acacactcag atatttctac aacaaagata tatgtgaaca caccagatat agttgataag
     2521 gctgtttttg aagcttttca gagaggattg aataagtgtg attgatatgt gttcttaaat
     2581 gtgttcttaa aaacaaaaga agcactttcc aaataattga aaagtgccag aaacgttgat
     2641 atataaagga tattaacgtt ttgagaattg tgaagcttta cgtgctttct taagacctgg
     2701 ttttttacgt tcgaccatac gtgcgtcacg agtaagtaag ccagcacgtt taagagctaa
     2761 acggaagtca ggatcaactt ctagcaatgc acgagcgata ccatgacgga tagctcctga
     2821 ttgtccagcg tatcctccgc cgtttacgtt aacaaatacg tcatatgcgc cttttgtttc
     2881 agtaacagcg aaaggttgat tgattacttc acgcaagtca gcatgtggaa tgtattcttc
     2941 aacatctttt ttatttacag taattttgcc agttcctggt actagacgta cgcgagcaac
     3001 tgcgttttta cgacggcctg tgccgatata ttgaacttgt gccaatgaaa ttccctccta
     3061 ttagattaag tttgtgatgt ctaaaacttc tggttgttgt gctgcatgtg gatgttctgc
     3121 tccaccgtat acgttcaatt ttgtaaattg tttgcggcct aaagtgtttt taggaagcat
     3181 accttttaca gaagtttcga tcaaacgacg agaattttta gcacgcaatt cgccagcagt
     3241 gattgatttc aatcctcctg ggtagttgct gtgacggtaa taaattttgt cagtcgcttt
     3301 tttacctgtt aatttcactt tgtctgcatt gatgacgatg acgaaatcgc cagtatccaa
     3361 atgaggtgtg aaagttggtt tatttttacc acgtagtaca gatgcaacaa ctgttgaaag
     3421 acgtcccaat ggaacatcag ttgcgtctac tacataccat ttacgttcta cttcgcctgg
     3481 tttggccata tatgttgtac gcacgttatt ttcctccaat ttaagttctg atgtttgaca
     3541 tacacttcga gtttccgggg ctctttgtgg ggcaaacaat accatttaac atattacccg
     3601 ctaatctagt caatgtcaac gtttttctgc ttgattgaaa aatttatatg aaactttact
     3661 tcttttcttt cttttatata aaagggtcgt ttcctgtcaa aatgaaaaac aaaaagaggc
     3721 tgtgacaaaa gtcttgtcac aaccccttat tttgaataaa cggtgttctc ctgccaactc
     3781 ctcggtcaca agtcacaata tccataaaac ataagaagct gttttttaaa attgtttctt
     3841 gtgactcgga gctgaacggc aggcttacaa cctcttggta aac
//
LOCUS       DACECZ010000162         3801 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.168, whole genome shotgun sequence.
ACCESSION   DACECZ010000162 DACECZ010000000
VERSION     DACECZ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.168"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..708
                     /locus_tag="IWO35_002573"
     CDS             <1..708
                     /locus_tag="IWO35_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0317599.1"
                     /translation="TIKEIIDELDISDMTARRDLDALEADGLLTRTHGGAQLLSSKKP
                     LEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPGTTLEQLALELKGRKGYKIRV
                     ITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMASTNLKAIRFAKAFVSANAVT
                     HNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYDFFNFYNLDQLDTIITDNQIS
                     PQHLEEFSQYTTILKAD"
     gene            713..1692
                     /locus_tag="IWO35_002574"
                     /pseudo
     CDS             713..1692
                     /locus_tag="IWO35_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1843..2007)
                     /locus_tag="IWO35_002575"
     CDS             complement(1843..2007)
                     /locus_tag="IWO35_002575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317600.1"
                     /translation="MSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIR
                     SNNSTQILTN"
     gene            complement(2122..2532)
                     /locus_tag="IWO35_002576"
                     /pseudo
     CDS             complement(2122..2532)
                     /locus_tag="IWO35_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812298.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            complement(2545..2901)
                     /locus_tag="IWO35_002577"
                     /pseudo
     CDS             complement(2545..2901)
                     /locus_tag="IWO35_002577"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3212..3667)
                     /locus_tag="IWO35_002578"
     CDS             complement(3212..3667)
                     /locus_tag="IWO35_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0317601.1"
                     /translation="MVKQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
BASE COUNT         1221 a          710 c          640 g         1230 t
ORIGIN      
        1 accataaaag aaataataga tgaactagat atttccgata tgacagcccg tagagacctt
       61 gatgctctag aagctgatgg acttttaaca cgtactcatg gtggtgcaca attgctttcc
      121 tctaaaaagc cacttgaaaa gacacatatc gagaagaaaa gtctaaatac aaaagaaaaa
      181 attgacattg ctaaaaaagc ctgctcttta atcaaagatg gcgatactat ttttattgga
      241 cctggaacta cacttgaaca actggcatta gaattgaaag gtcgtaaagg ttataaaatt
      301 cgtgtcatta caaatagtct ccctgtgttc ttgattctaa atgatagcga aaccattgat
      361 ttattgcttc ttggcggtga atatagagaa ataactggag cttttgtagg ttcaatggct
      421 tcgacaaatt taaaagcaat tagatttgcc aaagcttttg ttagtgcaaa tgctgttacc
      481 cataattcta ttgctacata tagtgacaag gaaggtgtga ttcaacaact tgccctaaac
      541 aatgctgtag aaaaattctt attagtagac agtactaaat tcgatcgata cgatttcttt
      601 aacttctata atctagatca actcgatacc atcattacag ataaccagat tagccctcaa
      661 cacttagagg aatttagcca gtacactact attttaaaag cggactagaa ttatgactta
      721 taaaaatatt gtttttgacc ttgatgacac tctttatgac catcaactcc cctttaaacg
      781 tagcgttgag aaatgttttc caacgattga tatccaacaa atagataaca tttacaaacg
      841 tttcagatat tggtctgatg ttgcttttcc taaatacacg aaaaaactca tcagtattga
      901 gcagcttaga atttttcgct gtcagaagac tatggaagaa tttggcatcg atagtatttc
      961 taggacagaa gctctagact tccaagctga ctatgaatat gaactcgatc agattacgat
     1021 gattcctgag atacatcaac tactgctggc tctacataaa aaccgcatcc caataggtat
     1081 tctaactaat ggccctgttg acttacagag tcgaaactcc aaaatattgg tgcctatcaa
     1141 tattttgaaa agcaaaatat catcattagt cagtccactc acggaggagc tgcagaaaat
     1201 agctatgaag gtaatgtaaa aaacttacaa agttaccgaa atgtccaatt atctaaaagg
     1261 gctatagaaa ttcttaaaga ggaaattgag ttaaatcacc aacatatgag attcaatcct
     1321 gattataaag ataatggatg gatttttact tccaaaagtc gtcataaacc cgactataat
     1381 ggaacacctc ttcattatag tgtccttaac aatttcctca actcacctga aaatggaaaa
     1441 ttaaatcgaa aaggtaatcc aaaacgagca ggaattgata ttgataataa acttagtttt
     1501 aataaacata ttacgaccca tatattccgt catacgcata ttagtttctt agcagagtaa
     1561 ggaatacccc tagaagcaat tcaagaccgt gtagggcatt ctagaggtag tcgtgtaaca
     1621 gaaatttatt tgcacatcac taaaaaaaca aagtatcaaa ttatacctat ccttgatact
     1681 cttacacaat aagtgtggca aatctgtggc aaaacataag aaaaaggctt gtccatattg
     1741 gacaaaccct ttattttcaa gcttatataa cctaattaag ctttaccagc tgattagaat
     1801 aggatgttgc atgttttact acgtcttttt catagagttt cactaattcg tcaaaatttg
     1861 ggtgctgttg tttgaacgta tcattatcaa tgataacaac atttcaagat caagtcctaa
     1921 aaaaccgcta tatttgccaa aattcgcttt taaataagca aattcgttct ctgtaataaa
     1981 tggtgtatct gttcttggat aactcaataa ttttgcttca tacagtcctt gcatagcttt
     2041 taaagtttgg ctcgctgtcg ctttataaag ctgattgact tttgattgca aactacttaa
     2101 agaaaacgaa ctcggactat tcgttttctt ctctttggtt tgaacatcag caattatccc
     2161 ctcttgattg cctattttag cttgtttaga agaaacaaaa gctaaaaagc tcctcttggg
     2221 ttttaaaacg ctgtgtgggg cttagaacgc ccttaaatga cccttgtttt acttagcttc
     2281 cacctcgaaa aaaggttctt ttttaaaatt ctctatggct tcctggcgct gaaaaataag
     2341 gtataaggtg ggcgtttgaa cacgtcctag tgaaaatgta ccttgtacgc ccttctgttg
     2401 taaatttaac gtatacaaag ggcttgcgtt catgccgatc aaccaatcgg caatttggcg
     2461 tgtttgcgct tcttgataaa agggatagta attcattcct ggttgcaaat tttgaaaacc
     2521 gcttcggatc acatcttttt ctaagctatt gatccatagt cttttaaatg ttttatcttt
     2581 tgaaaaggca tttgcattat ggataatcga ccaggcgata ttttcacctt ctctgtcgct
     2641 atctgttgca acaataattg tatttgcctt tttgagaagt tctgcaacaa ttttaaactg
     2701 ctttccctta tcttttgcaa cttcaaaatc gtatcgatca ggaaaaatcg gcaaagattc
     2761 aagtttccaa ttttgccact tttcgtcata atgacctggt tctgctaatt ccactaaatg
     2821 cccaaaacca aaggtgataa acgtttcatc tgtaaatagt gggtctagca aataactagc
     2881 tctaaatata aaaaacgcga tgaataatca taaatgtgat tgcttcgtcg cgttttttat
     2941 ttttggctgc tttgtgttga aaagcttccg cttttcttct agcctcaagg gataatttgg
     3001 ttagattcat gctcataagc atcattccaa tgtcagtagg tgcaacgttc ttgcctctga
     3061 catgggttct tcacatacca aatacacctt tcaatcgatc aaaaatagtt tctacgtcaa
     3121 ttttgcattg agcgtagagg gcttttccat aatcactttc aaggctatta tttaaaatcg
     3181 ctaaggcgtg aaggcaaatg ctgttcatat ctcaaaacgg ttttctgact cgcaattttt
     3241 tctctttgtc ttccaagttg attcctaaga gatcaccaac atgcgccatc accttatgaa
     3301 actgcttctc agtgatatga agatcataat gggctgtcga gggaaataac cactcagaat
     3361 tgatattttc ttgggccagc caatcatgat attgcaacaa gtcttgctga acaggcttta
     3421 aatataacgt gtttgcttta ccggtctttt tatcatgaat aaaggttgca tttttgacag
     3481 agccgtctgg attatagaca tctgattttt ttaatgtcat gacatcactc acacgtagta
     3541 gcgtggcctt accaacttga aaaacggtgt aatttcggcg accagcacgg aaactatcga
     3601 gaagtgtatc ttgtaccatc tttaagacgc tggagtcttt gatgggtagg acaatttgtt
     3661 tcaccatgat tcatgtatac ctttctgaaa ttaaataatt aaataaataa ttaattaaat
     3721 tgtttattta ttgatgacca ggaccgtatt tctaaacttc ttttatggtt tattatatca
     3781 cttaacaaaa aaacagcccc c
//
LOCUS       DACECZ010000163         3746 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.169, whole genome shotgun sequence.
ACCESSION   DACECZ010000163 DACECZ010000000
VERSION     DACECZ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.169"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(54..512)
                     /locus_tag="IWO35_002579"
     CDS             complement(54..512)
                     /locus_tag="IWO35_002579"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ0317602.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(683..1828)
                     /locus_tag="IWO35_002580"
     CDS             complement(683..1828)
                     /locus_tag="IWO35_002580"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ0317603.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(1841..2758)
                     /locus_tag="IWO35_002581"
     CDS             complement(1841..2758)
                     /locus_tag="IWO35_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ0317604.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3186..>3746
                     /gene="efp"
                     /locus_tag="IWO35_002582"
     CDS             3186..>3746
                     /gene="efp"
                     /locus_tag="IWO35_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ0317605.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1148 a          722 c          722 g         1154 t
ORIGIN      
        1 aatacgaaga taagatagct atattgcttc tcatctttac gttcttttag aaactatgct
       61 tctacttcag accattcatc gcgttttgct aaaaattcac gagccaaagc ttgtgcacct
      121 tctagtgtat ggctggcagc ccagccacat tgtgtttcat tgcttgcagg tacttcagta
      181 gcttcaagaa catctttcat tgttgcttca agaacatcta aaatgtcttc gtagctttca
      241 tgattcaata cagtcaagta aaagcctgtt tggcatccca ttggagacca gtctacgatg
      301 taatctgcgt gattgcggat tagttcggca gtcaagtgct caagtgaatg tagacttggc
      361 atgtccatgt gttctttatt tggttgttta aagcggacgt cgtatttaat gatgacatcg
      421 ccatgcgcac ccgtttttcg atcagctaca cgtacataag gtgctttgac ttttgtatgg
      481 tcaagattaa aactttcgac attcattctc atttgtaaga ctcctatctg tttgttcatt
      541 cgttttgatg cttttcacta aagaattatt tagtgaaatt tacaaaaacg tctttctact
      601 tgattctaac acttatcgtt tccaaggtgt agcaatctga aatatttttt agatttttct
      661 gctaagtcgt ttattgggac ttctagttct tgattctgtc aaatgcctga gctaaatcgt
      721 ttatgagatc ctgaccatac tcaattccta cagaaagacg gatcaagccg tcctttatgc
      781 cagcctcttc tcgtttttct tttggaatag acgcatgagt catgacagaa ggaatttcca
      841 ctaaactttc tacgccacct aaactttctg ctaaagtaaa tagttggagt gattcaacaa
      901 atggaatagc agattcttca ttttttaaag taaaagaaat cattccgcta aagccgctca
      961 tctgctgctt agcaactcca tggagttcat gatcgggtaa tcctggatag tagacctttt
     1021 ctactgctgg atgagaaaat agaaaatcag ctaccacaaa agcatttttt tgatgttcct
     1081 ccatacgaac acttagtgtc ttgattccgc gctgaagtaa ccagctatct tggggaccaa
     1141 gtacggctcc gatagcattt tggtaaaatc cgatctgatc ggctagatct ttatggttag
     1201 aagtcactaa accagctact acatcgctgt gtccgcctag atatttggta ccgctatgga
     1261 caacgatatc agaaccgaga tctaaaggtc tttgaaaata aggagtagca aatgtattgt
     1321 cagcaataac tatcaatcca tgttgatgag ccagagtggc acttttttct aaatcagtaa
     1381 tctttagaag cggattgcta ggtgtttcaa gataaagtgc ttttgtattg ggtttgatgg
     1441 attgttctat tttatcaaga ttactcgtat ctatgatcgt gtactcgaga ccatttttag
     1501 taagaacttt gtcaaataga cggaatgttc ctccatagac atcatctcct aataagatat
     1561 gatcgccagc ttgaaaaaga gaaaaaacgg catgaatccc agaaagacca gaagaaaaag
     1621 cgaatcctcg aacaccgcct tccaaatcag caatcagttc ttccaatgca aaacgagtag
     1681 gattaccaga tcttgaatat tcatattgtt taggctggcc gacgccatct tgccgataag
     1741 tggatgtttg ataaataggt acactgacag ctcctgttgt gggatcttca ctgatgcctc
     1801 cgtggatcaa ttttgtttga atgtgcatgt ttctttcctc ctataagtaa atgttttttg
     1861 ataaatagcg gtctgctgcg tctggaaaaa tcgtaacgac acgatgacca ggtggtaaac
     1921 gacgaatttc tcttaatgct gctgcaaaag ccgcaccact cgaacttcca actaatagtc
     1981 cttgttctcg tgctaactgc ctagtatagt taaacccttc tacatcagag attgtctcga
     2041 tctgattgat tgaaagggga gaaagaaacg gcggaatgaa ttctacaccg ataccctcaa
     2101 tttcgtgagg ggctggatca ccaccattta acacagagcc ctctggttct acaccgatta
     2161 ttcgaatgtc gggatatttt tctttcaaaa aggtagaagt tccagcaaag gttccaccac
     2221 tcccaattcc tgctacaaaa ctatggatat tttctttgat ttcatgaaag atttctggcc
     2281 ctaacgtatg atagtaagca gcaggattat ctttattttc aaattgaaga ggcagatagc
     2341 tgtttgatat ctcctctgca agttttttac ttttgctgat ggcaccgatg attccttgct
     2401 cactaggaga atggatgatc tttgctccca atgctttcat caattgttgt ttttctaagc
     2461 taaacttctc tggaacaaca aaaaccgttc ttaatttgta tgtgagtgcg gccagagcga
     2521 tcccaattcc tgtatttccg gcagttggtt cgatgatcgt cgttttttca ttgatttttc
     2581 cttgggcaaa tccttctttg atcaaataac tgcccaatct atctttgata cttcctccag
     2641 gatttaaata ttctagtttt gcataaatag cagaaccttt aggcatagaa tagtgctgat
     2701 tgtcaaagcg ataaagcggt gtggaaccaa tagcttctgt aattgtcgag ataatcatgt
     2761 gggcctccta aaaaaagata ataaaaaaag gagctgacta ttaaatcagc tccttgaaag
     2821 acatagagaa aaattgagta gataaaaaga taagttacct ggtactcatt ctatgaaggg
     2881 caattaatag acatgacaaa tagtatgctg cttttatgca ccatacaaca gcaacgattc
     2941 aattgttttt ggttgctgac tgtcatgtgt atccctccaa gtaaaatttt tattaacgat
     3001 agcatgaaaa aaaataaaag tcaaaggctt gaaagaaaaa aatatttcga tagcatcgtt
     3061 tacccgtgtt tatttttctg acaaataggc atgagacctt aaaagataga gaatactcta
     3121 gttttctggc cggttgtctg atacaataaa gttgattatg ataaagaaaa gaaggagcaa
     3181 aaataatgat ctcagcaagt gatttaagag ccggtatgac tttcgtacaa gacggcaaac
     3241 tgattaaagt tctggatgca agccaccata aacctggtaa aggaaataca gttatgcgta
     3301 tgaaactgaa agatgtccgt tcaggtgcaa catacgacac aacgttcaga ccagaagaaa
     3361 aatttgaaaa agcacatatc gacacaaaac ctgtacaata tttgtacacg atggatgata
     3421 cagctttctt catggatttg gaaacatatg agcaatatga aattcctgta gaaacagtag
     3481 aagaagaaat gaaatatatc ctagaaaaca tggaagtaaa aatccaattt ttcggtagcg
     3541 aagtcatcgg cgttcaatta ccaacaactg tcgtattacg tgttgtggaa acacaacctt
     3601 ccatcaaagg tgcaacagtc actggttcag gtaagccagc gacaatggaa actggcttag
     3661 tagtcaatgt tcctgacttc atcgaaacag atgaattgtt ggaaatcaat acagcagaag
     3721 gttcttatgt aaaacgtgcg tcaaaa
//
LOCUS       DACECZ010000164         3689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.171, whole genome shotgun sequence.
ACCESSION   DACECZ010000164 DACECZ010000000
VERSION     DACECZ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.171"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            73..2514
                     /locus_tag="IWO35_002583"
     CDS             73..2514
                     /locus_tag="IWO35_002583"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ0317606.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2844..3548)
                     /locus_tag="IWO35_002584"
     CDS             complement(2844..3548)
                     /locus_tag="IWO35_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317607.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYSLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         1182 a          652 c          809 g         1046 t
ORIGIN      
        1 aaaaaataac tggaacaaga gctatttgtt tttacagcaa agaatggtat atttttgtag
       61 gaggtggctt acttgaaaga agtcgttatg atagatgctg caagaacgcc gataggaaag
      121 tatcgcggaa gtttaagtcc atttactgca gttgaattag gcacacttgt gactaaagga
      181 ttattagata aaacaaaact gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc
      241 ctgcaagctg gtaatggtca aaatgttgcc agacagattg ctttgaacag cggtctccct
      301 gtagatgtcc cagcaatgac gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc
      361 ttagcacgcc agctgatcca attaggtgaa gcagaacttg tgatagccgg cggaacagag
      421 agtatgtctc aagcacctat gctgaaacag tatcagtcag aaacaaatga atatggtgaa
      481 ccaatttcca gtatggtcaa cgacggattg actgacgcat tttcaaatgc acatatggga
      541 ttaaccgcag agaatgttgc aacacaattt tctgtgagca gagaagagca ggatcgctat
      601 gccttgtcgt cccagttgaa agcagcacat gctgtcgaag ccggtgtatt ttctgaggag
      661 atcatcccag tcaagatttc tgatgaagac gtgttatctg aggatgaagc agttcgtgga
      721 aatagtacat tggaaaaact gggcacgtta cgtacagtat tctcagaaga aggaactgta
      781 acagcaggaa atgcttcccc gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa
      841 gaatacgcag aaaataataa tctgccttat ttagcaacca tcaaagaagt agcggaggtc
      901 ggtattgacc caagtatcat gggaatcgct ccaatcaaag ccattcaaaa attgacagat
      961 agatcaggaa tgaatttatc aacaatcgat ctttttgaga tcaatgaagc atttgcagct
     1021 tcttctatcg tagttagcca agaactgcag ttagatgaag aaaaggtcaa tatatatgga
     1081 ggagcaatcg cattaggcca tccaattggt gcaagcggtg cacgtatctt gactacatta
     1141 gcttacggat tgcttcgtga acagaagaga tacggtattg cttcattatg tattggtgga
     1201 ggtttaggct tggcagtctt gttagaagcg aatatggagc aaacacataa ggatgtgcaa
     1261 aaaaaaaagt tttatcagct tacgccatct gaacggcgca gtcaattaat agaaaaaaat
     1321 gtattgacac aagaaactgc gttgattttt caggaacaaa cactttctga agagctctcc
     1381 gatcacatga tcgaaaacca agtgagtgaa gtggaaatcc caatggggat cgcgcagaat
     1441 tttcaaatca atggcaagaa aaaatggatt ccgatggcaa cagaggaacc atctgtcatt
     1501 gcagctgcaa gtaatggtgc aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg
     1561 ctgatgcgag ggcaaatcgt tctatcagga aaatcggagt atcaagcagt gatcaatgca
     1621 gttaatcatc gaaaagaaga gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg
     1681 aaacgcgggg gcggtgttca agatatctct actcgggaat tcatggggag tttccatgca
     1741 tacttatcta ttgatttttt agtggatgta aaagatgcaa tgggcgctaa catgatcaac
     1801 tcgattcttg agagtgtggc aaacaagtta cgggagtggt tccctgaaga agagattcta
     1861 ttcagtattt taagtaattt tgctacagaa tcattggctt ctgcctgttg tgaaatccca
     1921 ttcgaacgct tagggagaaa caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag
     1981 gcaggggaat atgcgaagct tgacccttac cgtgcagcaa cgcacaacaa agggatcatg
     2041 aatggtatag aagctgtagt ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg
     2101 atccacgcct acgcggcaag aaatggatta taccaaggat taacagactg gcagatcaaa
     2161 ggggataaac tagttggaaa actaacggtg cctttggctg ttgccacagt aggcggagct
     2221 tccaatatcc tgccaaaagc aaaagcatct ttagctatgt tagatattga ttcagccaag
     2281 gaactagcac aagtaattgc tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca
     2341 ttagttacag aaggaatcca aaaaggccac atgggacttc aagctcgttc attagcgatc
     2401 tcaattgggg ctattggtga ggaaatcgag caagttgcga aaaaattacg tgaagcagaa
     2461 aagatgaacc agcagacagc tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc
     2521 aatatcttta gaagaataaa gcaagattta cagttatttt atttactgca gatcttgctt
     2581 ttttctctat cagaaaatat gtttattatt agaatttttt atagttaagg ttaggcatct
     2641 taagaaatca ggcaactcca agatatcttc aggtagaaga gaagtcgcct aacattgtag
     2701 gatcataggt gatttgttgg acaggtcaag ctgatatttg agcctgatgt tagaaggaat
     2761 ttctttttac ttgctgtaga aattattata tgactaaaaa ggctgggatt ttaaatccca
     2821 gccttttatg acaaatagaa tttttatcga tcagtaatcg ctgtcgctgc aaattgttcg
     2881 atcaaaggaa tcaaagtcaa tttgactttg tatttccgtt cgagtgcttt ttcgataacg
     2941 aactcatttt cagtagtcat agaaatagtt gctaatttac gaggttcatc agcatttttt
     3001 tcattaataa ctacttcatg ttcattttgt tctgtgtaaa atttaggata ctcagcaacg
     3061 gcttttgaaa aatcagcaaa acttaaagcg gcattcgtat cagttgagcg tgtattttca
     3121 gttgtgtccg cttttggttt ttccgttttt tcttctggca cactgactgt ttcttctgta
     3181 actggtgtcg caacagcttg cgcagttggt tcatcgtttt tcaatcgttg atcattccag
     3241 taagacacga tttcaataag aagattgtca cggaatcgtt cgaaaatccc gttttctcct
     3301 gaacgaattc tctttaatac agtattgaag ttgtcatcga ttggcacgtt gatccgtgta
     3361 tcttcatttg ttttcccagc taatccgatc gttaagattt gacggttttt gggatcaagc
     3421 atgacttgta acggcaaact ataacgtcgc aagctctctt cttctagaga acggaataat
     3481 tgtcctctta caccatccaa tgaaggacgg aattgattta ataatttttg tttctgtttt
     3541 ttcttcatat gttctctcct tcgctcatac cctttcgatt ataagaaaaa aacagccaaa
     3601 tgaaaaggag taatgcaaaa aaacttgcgt atacgcaaat tacaacagaa aaatttgcgt
     3661 attttcccgt tttttgaaag aaaaataaa
//
LOCUS       DACECZ010000165         3679 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.172, whole genome shotgun sequence.
ACCESSION   DACECZ010000165 DACECZ010000000
VERSION     DACECZ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.172"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..298)
                     /locus_tag="IWO35_002585"
     CDS             complement(<1..298)
                     /locus_tag="IWO35_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0317608.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLV"
     gene            complement(428..1012)
                     /locus_tag="IWO35_002586"
     CDS             complement(428..1012)
                     /locus_tag="IWO35_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0317609.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(1343..1576)
                     /locus_tag="IWO35_002587"
     CDS             complement(1343..1576)
                     /locus_tag="IWO35_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ0317610.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(1591..2541)
                     /locus_tag="IWO35_002588"
     CDS             complement(1591..2541)
                     /locus_tag="IWO35_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0317611.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(2538..3380)
                     /locus_tag="IWO35_002589"
     CDS             complement(2538..3380)
                     /locus_tag="IWO35_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0317612.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(3382..>3679)
                     /locus_tag="IWO35_002590"
     CDS             complement(3382..>3679)
                     /locus_tag="IWO35_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0317613.1"
                     /translation="VLLARCLAQNADFIFLDEPFVGIDLVSERIIMDTLKKLKHQGKT
                     VLIVHHDLSKVKKYFDNIILLNRNLIAHGSVESVFNEENLKKAYGDTIFIGEGE"
BASE COUNT         1267 a          674 c          446 g         1292 t
ORIGIN      
        1 taaccaacaa ccatgagatc attacgaccg gcaacattcg aaatacaata tataaggatg
       61 tcgcaaaaaa tatagtaaaa aactctatct tgatagcatc aataattttc attactaaaa
      121 acagaactga ataaatcaag atgaaataaa tacatgtctt tattttacga aacatcaata
      181 ctaaaactgt agcaataatg aaacacccca cttgaaatat atttcccttt cctaaaaaaa
      241 taatgaccgg aaaaaaaata tttaaagtaa tcaaggttaa cggattgatt tttgacactc
      301 tttcacctcc aaaaaataag atcgcactgt tatcttaagc tgataaaaat gcaccgttat
      361 tcagataaca gtgcgatctt atcaaaaagc ataactgaga acaattctca atgcaagctt
      421 tttttaatta actcagtaat tcaatccacc caggaacaga aaattgatac aatcttctgg
      481 ttaatagcat agtcttttct tttgaatagc tatgcttcaa tatatcatag aatgtagcgt
      541 agaatgagta aatgtagaag tggatttctt cttcattaaa gactaaattt tgattgatca
      601 ttccgatagt ctttaactta tctagacaac agactgtctt tttcgtcaca aatgatgtaa
      661 ttttttcaac aatatcttga tatttggttc cagctgattg gaataaaatc aaatcgaata
      721 tacgtttatt ctcaaaaata taattaataa atggatctaa ttcattgaag ttctctctcc
      781 aacttttctc tatatctata ttttctaagt ttaagaggta tttggtaaac tcttcattat
      841 acatattaat aagcgcatta atatgcggct gaataatttc ttcaaataac gcttccttag
      901 aagtaaaatg tttataaaaa gcacccgtag ttacgcctgc ggtcaggcac agttgcctta
      961 tcttagtttg ctgaaatcct tctttttcaa aatggcttag tccactttct attaactttt
     1021 ggtgagttat aatataatct ctggtttcta tgggtacttc ctcctaataa gatgaattaa
     1081 ctttattatc ttacacagga cttgaagttt caatcgttaa cgtttattac tgaactcatt
     1141 ccttagagct aaactaatga gaaagagcag aataactgat acaaaaagca atcctacaag
     1201 aattaataga ctcatctaaa tcattcacct tttctaaaat aatccgagaa agatttaaaa
     1261 aaattatatc cagctattag gataagtcct atcataatca ctccaatagc cattttctta
     1321 tcctccctaa aaataaagtc ttttacttaa aacctttttt gtaatcgtca ataaattttt
     1381 tccctttatc agttaattga aatcctatat ctaaatagtc ctcatatcta agtttagcca
     1441 gcatctcact tacagatgct ggactaacac ttagaacttt tgctaaatca ttatttttta
     1501 atttcttttt tctttttgat aattcctgaa ttgctagtaa ataaattagt atagtagtcg
     1561 ttttaatttg taccatacct accctccaaa ttatttcgaa aggccttcag caattttatc
     1621 caggttccat ttcatcatcg catagtaact atcaccatct tgtccttttt ctgcaattga
     1681 atcagtaaaa atcgttgaat agataggaat attggtatct tttgatactg ttttcattgg
     1741 tctatcatcc acactgcttt ctacgaataa ggagggaact tttgttgtgc gtaatttttc
     1801 aactaagtgt tttatttgat ctggtgttcc ttcttcttca gtgttgattt cccaaatgta
     1861 agcagaaggc acattatacg ctttcgagaa atatttaaag catccttcac ttgtaacaat
     1921 cattttttta tcattcggaa ttgaagcaaa tttagattta gcttctttgt ctagagaatc
     1981 caatttttca atatacttat ctagattttc tttatagaat tttttattat caggatcttt
     2041 ttccgctaac tgtttttcaa tatttttagc gtaaataata ccgttttcta aatttaacca
     2101 agcatgagga tcttccttcc ctttctcact ctgaccctct aagtaaataa catctatgcc
     2161 atcacttgct gcaaaatagt ctttgttttc ctctttgttc gcatttttaa ctaattttgt
     2221 aaaccaagca tttcctccag tttccaagtt aacaccgtta taaaaaatca aatctgcttt
     2281 tgaagttttt tgaacatctt caggcaaagg ttcatattcg tggggatctt tcccaatagg
     2341 tacgatactg tgtaaatcaa ttttatcttt tgctatattt tcggtaatat ctgctaaaat
     2401 cgaattagta actactactt ttaatttttc gttcgaagcc actttatctt tgtctttact
     2461 atttattgaa catgccccga atataaaaag agcaatcaaa atccccagac ttcccagtac
     2521 tgctttccat tttctcatca taacgatctc tccttctttc ttaaaaattt ttgttttggt
     2581 gagattagaa aactaattac aaataagaat gccgctgtta aaacaatact tgatcctgct
     2641 gctatgttaa aactataacc aataaacaat ccaagcaatg atgaacttgc tccgactgtt
     2701 gcagaaataa atatcatagt tttcaagcta tttgcatata aatatgctgt agctgctggt
     2761 gtaattaaca ttgcaacaat taatatagtt cccacacttt gcatagctgt tactgctact
     2821 aaagttaata gaaccatcaa tagataatga taaaaactga cattcatccc tattgctttt
     2881 gccattaatg gatcaaaaga agtgattaat aattctttaa aaaatagaac tactataacc
     2941 aacactaaaa ccgaaacgcc aattgtaatc cacttatcaa tatcttgtac agctaaaata
     3001 tttccaaata aaatatgaaa taaatccgtt gagctatttg caactcctat aaggataact
     3061 cctagtgcta aaaaagaact gaaagtaata ccaattgctg tatctccttt tattacacta
     3121 ttgcttttta taaaagtaat aatgatagaa gctaatagac caaatacaat cgcaccaata
     3181 aaaaaattaa tacctaaaat ataagaaatt gctactcctg gcaaaacagc atgagaaata
     3241 gcatcaccca taagagacat tcctcttaaa ataataaaac aacctactgt tccagaaaca
     3301 atccctataa ctacagatgt aattagagca ttctgtaaaa agtgaaagtc atacagacca
     3361 ttaataaaat tttgaagcat attattcccc ctctccaata aatatagtat ctccataggc
     3421 tttttttaaa ttttcttcat taaatacaga ttctacactt ccgtgagcaa ttagatttcg
     3481 attcaaaaga ataatattat caaaatactt ctttaccttg cttaaatcat gatgcacaat
     3541 tagcactgtt tttccctgat gtttcaattt ttttaacgta tccataatta ttcgttcact
     3601 aactaagtct atccctacaa atggttcatc taaaaatatg aaatccgcat tttgagcaag
     3661 acatcttgct aaaagtaca
//
LOCUS       DACECZ010000166         3645 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.173, whole genome shotgun sequence.
ACCESSION   DACECZ010000166 DACECZ010000000
VERSION     DACECZ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.173"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            90..1079
                     /gene="galE"
                     /locus_tag="IWO35_002591"
     CDS             90..1079
                     /gene="galE"
                     /locus_tag="IWO35_002591"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ0317614.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            1091..2575
                     /gene="galT"
                     /locus_tag="IWO35_002592"
     CDS             1091..2575
                     /gene="galT"
                     /locus_tag="IWO35_002592"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ0317615.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            2599..3579
                     /locus_tag="IWO35_002593"
     CDS             2599..3579
                     /locus_tag="IWO35_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0317616.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         1231 a          659 c          804 g          951 t
ORIGIN      
        1 ttgtttgttt tcaccataaa agtaaacttc gttttttaaa ttatatagaa tcaacaaaca
       61 caattttact aatcaaagga gtgtttttta tgacaatttt agtactaggc ggagccggtt
      121 atatcggttc gcacgctgta gaccagctag ttcaaaaggg gtatcaagta gctgtggtag
      181 ataatttgct cacaggtcac aaacaagcag tacatccaga tgctcatttt tatgaaggag
      241 acatccggga taaagaattt ctacgttccg tatttgaaaa ggaaccgatt gaaggagtaa
      301 tccattttgc tgcaagctct ttagtaggag aatctgtcga aaaaccattg atgtatttca
      361 acaacaacgt atacggtatg caaatcttac ttgaagtcat gcacgagttc aacgtgaata
      421 aaatcgtatt ctcttctact gcagcaactt atggagagcc gaaagagtct ccaatcacag
      481 aagatacacc agctaatcct aaaaatccgt atggtgaaag taaattgatg atggaaaaaa
      541 tgatgaagtg gtgtgaccaa gcatatggca tgcgctatgt cgctttgcgc tacttcaatg
      601 tagccggagc aaaagcagat gcatctatcg gagaagatca tacaccagaa acgcatcttg
      661 tcccaatcat tttacaagta gctcttggtc aaagaaaagc cttggctgtt tatggagacg
      721 attatgatac accagatgga acatgtatca gagattatgt tcaagtagaa gatttgattg
      781 ctgcacatat tttagctttg gaatacttga aagaaggaaa tgaaagcaac ttcttcaatt
      841 taggcagcag caaaggatac tcagtcaagg aaatgctaga agcagctaga gaagtgacag
      901 gaaaagaaat tccagcagaa atagcaccaa gaagagctgg agatcctagc cgattagtcg
      961 cttcaagtga aaaagcgaga gaggttctgg gttggaaacc tgaatacaca gatatcaaag
     1021 caattatcaa aacagcatgg gattggcatg tcagccatcc aaatggttat gaagaatagg
     1081 ggaatgagat atgtcaatca gtcaaacgat ttgtgatttt gccacacttg cgatcgaagc
     1141 tggcggatgg atggaaatgg accgcttata tttacagaat cgtcttttag ggatggtagg
     1201 agaagatgct ctagaaagca ttgaagtaag accagttaag gaagaatctg tagacttatt
     1261 ggatcagctt gtcgctaagg caagagaaaa tggtgttctt tcagaattgc cagcagaaca
     1321 agagattttt gaagcgcaat taatggattt tctcacaccg ccaccttcag tcgtcaatgc
     1381 attctttgca cagcactatt cgaaaagccc agaggaagca actgagtatt tctttaattt
     1441 aagtaagaaa aatgactaca tcaaaacaag agccatcgca aaaaacatca aatttccggc
     1501 agagtcggag tatggtgaac tggaaatcac aatcaattta tctaagccag aaaaagatcc
     1561 aaaacaaatc gcagcagaac gcgaggcggt aaaagtagac tatcctacct gcatgctttg
     1621 catggaaaac gaaggatata gaggtcgctt gaactatcca gcccgcacca atcaccggat
     1681 tattcgaatg aatctagacg gtgaaagctg gggattccaa tattctcctt atgcttatta
     1741 caatgaacat tcgatcattt tatcagaaca acaccgcccg atgaaaatca caaaggaaac
     1801 atttgctaga ctgcttcgga ttactgaagt cttgcctcat tactttgttg gttcgaatgc
     1861 tgacttgccg attgttggag gatcgattct ttctcatgat cattatcagg caggacgtca
     1921 tgaatttcct atggcaaaag caccgataga aaaacttatc aaactagcag attatccgca
     1981 agtcatcgca ggagtcgtga aatggccgat gtctgtgatc cgtttgcaat caacagagaa
     2041 aaaattgttg attgaagcag ctaactcagt attggaaaag tggaaagcat attcggatga
     2101 aacagtatct atcagagctt tttcagcaga tggtacacca caccatacga ttacgccaat
     2161 tgcacgaaga agggacaatt actttgagtt ggaccttgtc cttcgagaca acaatgtttc
     2221 agaagaacat cctgatggga tttttcatcc acataaagat gtccaacata tcaaaaagga
     2281 gaacattggg ttgatcgaag taatggggct tgctgtttta ccacctcgtt tgaaaccaga
     2341 actagtagaa gtagagcatt tcttattagg agaagaaaac cagatggctg aatatcatcg
     2401 tccgtgggca gaacaattaa aaaaagaaca tcctgatttg acgaaagata cggtcaccga
     2461 tgtcgtacaa aaatctgtag gtcaggtttt tgcacgtgta ctggaagacg ctggagtatt
     2521 caagcgagat gaaaaaggac aggcagcgtt tgaacgattt actgcaattt tataaacgta
     2581 aaaaaggagg agagacaaat ggcaacaatt aaagacattg cgcagttggc aggagtttct
     2641 cctgctactg tttcaagagt tctcaactat gatcctgatc tttccgttgg cttagccaca
     2701 aagcaaaaaa tttttgaagc agcagaacgc ttaaattata cgaaacatcg taaaaatgca
     2761 gcaaatacta agcaaacgct tcgcctgatc cagtggtacg acgatacgga agagcttgaa
     2821 gatctttatt atttatctat tcgtctagga atcgaaaaaa aggcagaaga agcaaacgtt
     2881 actttattga aagaaacgtg ggattccatt agtgatcttc cgacagatgg aacgatcgca
     2941 ctaggaaaat tcgacgaagc tcagttatta gaaatgaaag gatcccaaaa tgccttgctt
     3001 ttggtagatt ctgacggcac ttctcaagga atcgactctc tagttgtcga ctttaagtca
     3061 agtgttcaaa aagtcattga acattttgtc tcccataaag cgcaacatat cgctatgctt
     3121 gctggaacag aatatacgaa gaaaaaccat cagcggctaa aagatcccag aattatggca
     3181 tttcaacaag ttttggcaga aaaaggctac gacgaaggtc caattatcga agcagatttt
     3241 tctgtcgagt caggatatca agcggtgaag cagtatttaa aaacgaatcc gcatgttcct
     3301 gatgcgcttt ttgcagctaa tgatgcatta gcgattggcg cactgaaagc gattcaagaa
     3361 aaaggtttag tggtaccaga agatatttct gtgatgggtt tcaatgatgt cagtgtagcg
     3421 aaatatgtaa cgccctctct ttctactgta aaagtttata ccgaatggat gggagagcta
     3481 gcagtggaga cgattcttca attaagtaat tccaaagcac cggtttcaag aaagattatt
     3541 gtggaaacag agttgattat tcgagaatca acgaaataaa agatggaaaa gctgtgggga
     3601 aatgaagtga taccgcagct tttttgtgtt tcaggaaggg gcaaa
//
LOCUS       DACECZ010000167         3558 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.174, whole genome shotgun sequence.
ACCESSION   DACECZ010000167 DACECZ010000000
VERSION     DACECZ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.174"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            253..1224
                     /locus_tag="IWO35_002594"
     CDS             253..1224
                     /locus_tag="IWO35_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0317617.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            1256..2653
                     /locus_tag="IWO35_002595"
     CDS             1256..2653
                     /locus_tag="IWO35_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0317618.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            2812..3435
                     /locus_tag="IWO35_002596"
     CDS             2812..3435
                     /locus_tag="IWO35_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317619.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         1283 a          565 c          765 g          945 t
ORIGIN      
        1 aaaaaggcaa aaaatccgcc actttcagaa agcgttttaa aaaagtgttg caatctttta
       61 aaaaaatgct atattaaaaa tgaaccggtt caaatacttg tttaagtata attaatagtg
      121 agaggaagtt gctctactga agactttagt tatatggata ttgatgacaa gcaacactat
      181 gcaaagaatg cggtcatcat aatcgaacaa caagaaagag aaatcaaatg taactggcag
      241 gagtgagaat gtatggcatc gatccgtgat gtggctaaga tggctggtgt ttcagtggga
      301 acagtttccc gttacttgaa tggtcagcaa ctgaaggaaa agaacatgaa aaaaatagca
      361 gaagcgattt cagcgttgga ctataaagaa aacatcattg ctaaagggtt gaagaataat
      421 cgaagcttct ctattggact tttgataaac gggatttcca gccgttttgg gtcggaagtt
      481 gtctcaggga tcgaaagagt agctgaagaa aatgggtata gcttattatt aagcgggttt
      541 gccgatcagc cagaaaaaat tgaacaaaaa attgaatacc tgatgaagca tgtgatcgat
      601 gggctgattg tcttcttatc cgaagaagag tggtctggat ttgaaatgct cacaaaaatg
      661 agtattccag ttatcgcatt gaattgtcca gaaggaccag caggtattga tacaatatta
      721 gtgaatgatc gagaaagtgt agcaaaagtg atcgctcatc atggtgaaca aggacataaa
      781 aaaatcggat ttattgctgc cactcaaaca gactacgtag caagagaacg tttagcagga
      841 gccaaagaag cggtccaaaa tgatccatcg atccaattag aggtctttac aggagactat
      901 tctagaaaaa gcggctatta cggggcaaaa gcattattag aaaaaggggc aactgctatt
      961 tttgtcagca attacaatat gtcgattggt gccatcgaat gtttcaatca agaaggtgtc
     1021 agaatcggga aggatattgc gttcagtcac tatgattatt tagataaaaa cgatctgagt
     1081 atccttccga aaatcacgat cacgccacca acagataaaa tcggggaacg agcagcagaa
     1141 cagctgttaa aacgaatcaa aaaagaagag gaagaaaatg aaaaagtgat tgtgatggaa
     1201 aatatcatac atggaatcaa ctaaaacgaa ctaaaaaaga aaaagaggtg ctttgatgaa
     1261 aaaatatcaa tttccagcaa atttttggtg gggttctgca gcaagtggcc cacaaacaga
     1321 aggaaggatt tcaggcgatg gaaaaggaga gaacattttt gattattggt acaaaaaaga
     1381 accgcaaaag ttctttgatc aagtaggacc tgaaaaaacc tcgcaagttt atacgaagta
     1441 tcaagaggat gtacagttga tgaaacagac tgggcataat tcatttcgaa catctatcca
     1501 atggagccgt ttgatacctg atgaatctgg aacaatcaac gaagaagccg ttcgtttcta
     1561 tcatcgttat tttgatgagt tgattgaaaa cggtatcgaa ccctttgtga atctgtatca
     1621 tttcgatatg ccgatgtatc tgcaagaaaa aggcggatgg ttgaaccgag aaacggttga
     1681 agcctatgaa aaatatgcca aaagttgttt tgaattgttt ggttcgaaag taaaaaaatg
     1741 gtttactcat aatgaaccaa tcgttccagt cgaagcaggt tatttatatc gatggcatta
     1801 tccagaagag tcagatatga aaaaagcgat ccaagttggc tatcatgaag cgttggcttc
     1861 agcgttagcc atcaaatctt accatgaaat ggagcaaggt ggtgttattg gtatcatctt
     1921 gaatctgaca ccaagctatc caagagacga atcaaaccct gaagatgtta aagcagcgca
     1981 aatagcagat gcgttcttca atcgttcatt ccttgatcca gctgtaaaag gcgagtttcc
     2041 aagtgaattg attgagatag taaaagaact tgatctcatg cctgtgatcc atgcagagga
     2101 tttgacggtt atcaaagaaa atacagtaga tcttttaggt atcaattatt accagccacg
     2161 ccgggtaaaa gcaaaagaaa caccaatcga tactaaaaac ggtccaatgc ctgaggatta
     2221 tttcgacaat tatgaaatgc ctggtcgtaa aatgaatcct tatcgcggat gggagatcta
     2281 tgaacaagga atctatgata ttttgatcaa tgtacgagat cattatggaa atatcgactg
     2341 ctttatttct gaaaacggta tgggtgtaga aaacgaagca cgatttatca aagaagatgg
     2401 catgatccac gatgattatc ggatcgaatt tgtccaatct catctgaaat atgtccatca
     2461 agccattcaa gaaggtgcca attgtttagg ctatcatatg tggacatgta tggataactg
     2521 gtcgtggacc aatgcatata agaatcgata tggctttatt tcagtagact tagcacaaga
     2581 tggaaaaaga acaatcaaaa aatcgggtca ttggttcaaa gacgtatcag ataaaaacgg
     2641 atttgaggct taagaaagaa tagttgagag caaatgcgtg aaattctcca ttcttaccat
     2701 tttgtgaaaa agaagttcaa ttatttacga tctttttcaa cttttttgtc tttctatttc
     2761 attttttgta tataatagaa tagtcaaaat taaataggag tggaggaaag catgaggaaa
     2821 aaaagaccgt ttcttctctt ttggttattc ggttttttct tactgcttat tttatccgca
     2881 gcattttcag gaaaaatgac agcagatctt gctcagaaag aaacagaaaa aatcgtagca
     2941 agcaaagaat cagtagataa tttcaaaaaa gaattctcaa cttattggtg ggcggagtat
     3001 aaaataactg ccaattacga aaaaggcgaa ttggacattt atttgccaaa aggaaaagaa
     3061 gaaaatcaat ttattaatat cgatgacgta cttagtgcga tttctctcag acaatatcaa
     3121 tttaaagaca agcatttgac cgttttgctt ttgactcatg atggcgacaa agtagcccgt
     3181 ttgaaaaatg gaaaagtgat caccgcttat tctgaaaaag tagacaaaaa agtggtcgcc
     3241 gatcgtttag cggaatggaa aaagacaaat gaacaagtca agaaaacaac tgtctcttct
     3301 caaaaaaacg agtcgacagg aacaagtcct tcagattctg gcagtggaca aacgataacg
     3361 ggaagtgaag aaaatcaagc ctcaattatt acgaatgagt catcacaagg acaatatcaa
     3421 tcctatggac aatgaaaaac agaggttgtg aaatcagcgt tttgacctga gtattagaga
     3481 aacaaacggc aaaatagttc ctcatatttt gccgtttttt gagctaatac cgaaggtcag
     3541 ctgattgaac accgtgta
//
LOCUS       DACECZ010000168         3433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.175, whole genome shotgun sequence.
ACCESSION   DACECZ010000168 DACECZ010000000
VERSION     DACECZ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.175"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(718..1674)
                     /locus_tag="IWO35_002597"
     CDS             complement(718..1674)
                     /locus_tag="IWO35_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317620.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(1783..2028)
                     /locus_tag="IWO35_002598"
                     /pseudo
     CDS             complement(1783..2028)
                     /locus_tag="IWO35_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2173..2595)
                     /locus_tag="IWO35_002599"
     CDS             complement(2173..2595)
                     /locus_tag="IWO35_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317621.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(2734..3164)
                     /locus_tag="IWO35_002600"
                     /pseudo
     CDS             complement(2734..3164)
                     /locus_tag="IWO35_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT         1054 a          733 c          555 g         1091 t
ORIGIN      
        1 aaaaacatgt gaggtcgtaa acaaagattg aactatctac attcatactc tataatctgt
       61 ttatttgcag actgataata gcaaaagaaa acttataatc aaataatatc aacatttgat
      121 cacataattt gagattgaat gctgatttac ctatgtttta cgtaatggtc tttagttatt
      181 tttgctcata aaatgcataa aacacgcgtt tttatgtgtg cttgaaattt cttggctcta
      241 taatttctaa gactgataaa aatattctat cggttctttt tgcaaataaa aaaacatata
      301 gaagctccat tagattgaat caccacaaca caatcaaagg agcattccgt atgcttgatt
      361 ttcattctat aaaactactt ttaggaatca aagaaaatca caacggttca aactaaaagc
      421 aagaatatcc gaagtaccat tgtttcatcg atcatgagaa aagcattcac ctctgtctgc
      481 agatgttctt atatcgctct gtcagtccta ttaataaagt actgttttgt tttcaaaaaa
      541 actattacga gtacttttta agagaatgcc ttgcaaatat tcatcacaca ccgttatata
      601 agtcaaatag tttctttcag tcgtcaagaa aagattctcc tgtccttggc attatcagtt
      661 aagtagcaac ttaattctcg gacttgttct actctagcaa aatttatttt catcaactta
      721 cgtccaaatg tttttttgaa taattacttc atcgcgggtc tggaagactt gcctcattcg
      781 ataagtcact agcttttggc gtccttcagg atttctagta tacaccaact gacatttggg
      841 cagcgacgat tgatttaatt cgtttagaaa aaggttagcg atttgcttat ttcggccaaa
      901 gcgttctggt accgtcagaa ctatcttttt cccttcgatt aaataccgcg gattctccag
      961 cggtgctaaa agctccctca tagcttcagt aaaaagctgt ccttctcgat agccagcatt
     1021 ctcaagatag catctaaatt cctgctgatc agccgctacg attacttttc tatcactaac
     1081 ttcaccaagg cgctgcaacg tcgtttgaac gcatatggcc aagctttttt tgcgggcaca
     1141 ttccttttcc catagctttt gtcgcctacg aatttggaaa tattccagca agttaccttt
     1201 aaatatagct gaccagaagc ttagaggctc tatatttttt actactatcg gcaatgaagt
     1261 ttcttgtttt tgctttacct gatcgacttt cctaacgacg attggcatag ataggtgcca
     1321 gccatcagct aaagcagtac tccattgttc tttcaaccga cttcgttcac aagaaaaata
     1381 gaacatcgac tgagtataag tttccatcgt ggtggtcgta tattccagtg gtaaagcaaa
     1441 gcgatcgcca ccactctcaa tttttggtga agattcaaag gtcagacctg aaatattgcc
     1501 caaacgccgt agcattcttt tcaaatcagg ctgttcagac agaggaactc ccggtaatcc
     1561 aatcgctaaa tgccaaatgt tcgttacttt attttgtgca cctaggcgta agccccgccc
     1621 tcgtagttgt tgtgtttgca caaaagtgga agcttgatta gccaagatca acagattaat
     1681 tgcaggacaa tcccagcctt ctcctagcaa actaacggtt cctaccaaaa tattcaactt
     1741 cttttcggtt aataggcgag taactgcttg catcaatgct tggcggtttt tgtcatccac
     1801 cgtcaaataa aaataacccg acatataagg atgttcattc atacccgtcg ctagttctgc
     1861 cagctcaaca gttactagca ttttcggtaa cagtaagaac tctccacaga tggcaccgat
     1921 ttgctgagtg ttacctaata gacgaacaac ttcatcaaac aaaggaacca cgccataagc
     1981 taaggtacca tcactgcctg ctaatacctc cttgcaaata cgatccatca aaatgaccgc
     2041 tgtcagttct tgcttcagct gaacttcttc ttttaacaaa atttgacaaa ctgcttgctt
     2101 tttctcagga atcgcctgac taccaacagt gtctggatac tctggaaaca aatgcagcga
     2161 ctcatcaaac aatcatccag cttttttcaa ttgataaaaa ataggcaatt ccggttgatc
     2221 ttcaaacatc tgctgataaa tagtgatcag ctctttttta cctaaagatg gaactgaagc
     2281 ttttttacct tgctgcttag aactgcctaa tagctgccaa tgttcgggta tcaattcata
     2341 gttatgatga ttcaagtaaa ttaatgcact agtgaacagc tcaaaatttt cataaatttc
     2401 cttcgaatgc tcaagcggat gctgaacaaa ttctaaagaa agcaaataat ccaccgtctc
     2461 ttggctttcc attaacgctt ccaccagctc attttgtttc tctaaaaagg taaaataatg
     2521 ctgagtagtt tcctgattag ccggaatcaa atatagaaaa tcctgatgag gacatagaac
     2581 ctcctccttt accaactcgg gaatgctaat ttcttcgtca atcaggccat tttaactcaa
     2641 tatattgctt ccactccgtc tgagagctat caatcggagg cgtggctgtc aaggaaatcg
     2701 tcaacagatt cggttgtttt ttcttcaagt caatcaacaa ttcaccccag ctttttttta
     2761 aatgatgagc ttcatcaaaa aactaacagc tccagctgat caccataagt tggctcctcc
     2821 ttcagtcggt tgtaaatggt ctgatacgtt tccactgtaa tatgactagg atgttcaatc
     2881 gccccagaaa gatagcaact atctgctgga tttactaaga aatattcctc gattgttgtt
     2941 aaccactgtt tcttcaatat tagggaagga accaaaatta aggttttaca atccaaacgt
     3001 cggatcatct caattcctaa aaaggtcttg ccagcccctg gtggagccac tagatggact
     3061 gaattgttat ttaaataagt agcaaagtta gtcaatatac gcttctgata gctacgccac
     3121 tgaatttttt catcagatag ccacatccca ggtccccctc tcacgtattt tctctatcat
     3181 aatctaaatg aatacagcaa aaaaaagatt tcttcaagaa tatgtgtttt tatagtaata
     3241 ttttacttac tctaaactca aatgaaaagg taaaaaaact ggtagtacaa tcttttaaat
     3301 tctcattgtt ttgtacgttc tttactatca aaaaaacaaa aaaaacgcgt ttctacgcgt
     3361 gaccaaaatt tttagctcta taatttctaa gactgataga aaatcattct gtttttgaag
     3421 tcttttttca taa
//
LOCUS       DACECZ010000169         3427 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.176, whole genome shotgun sequence.
ACCESSION   DACECZ010000169 DACECZ010000000
VERSION     DACECZ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.176"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..2177)
                     /locus_tag="IWO35_002601"
     CDS             complement(<1..2177)
                     /locus_tag="IWO35_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317622.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3117)
                     /locus_tag="IWO35_002602"
                     /pseudo
     CDS             complement(2849..3117)
                     /locus_tag="IWO35_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          967 a          755 c          539 g         1166 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactatcc ataaaaaatt gcataaatga caatagtatt tatattagtc
     3301 atactgcttt ttaacacagc aaattccaga aaaacattcg ttacatattt gtgaattttt
     3361 ccaaaaaata acatttttat aaaacgacga aaaataaatc actaccagtc aacttctata
     3421 atattac
//
LOCUS       DACECZ010000170         3349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.177, whole genome shotgun sequence.
ACCESSION   DACECZ010000170 DACECZ010000000
VERSION     DACECZ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.177"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            191..775
                     /locus_tag="IWO35_002603"
     CDS             191..775
                     /locus_tag="IWO35_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0317623.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            1146..2471
                     /locus_tag="IWO35_002604"
     CDS             1146..2471
                     /locus_tag="IWO35_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0317624.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            2464..2814
                     /locus_tag="IWO35_002605"
     CDS             2464..2814
                     /locus_tag="IWO35_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317625.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            2811..2990
                     /locus_tag="IWO35_002606"
     CDS             2811..2990
                     /locus_tag="IWO35_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317626.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQLISMNPFLFLQIGVDTRI
                     MRVLNYYCKRNNKRK"
     gene            3002..3295
                     /locus_tag="IWO35_002607"
     CDS             3002..3295
                     /locus_tag="IWO35_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ0317627.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSMNMTDVYEKLLILENEYDQFNSNQQRKVKKQID"
BASE COUNT         1171 a          505 c          666 g         1007 t
ORIGIN      
        1 ctagtcgaca ttcttttttt ctaattgaaa gtgttcataa aatcacccat ttctggacac
       61 ttcctttttt tgctttattt ttcaaaaaaa caattcattt catgttcata aacccataac
      121 aatatctatg ttcagttaaa ctatatggat aaagttatgt tacaataaaa ttaatgaagg
      181 agtgattttt ttgaaaatag gatacgcccg ggtatctact ggtttacaaa atttggattt
      241 acaagaagac agcctaaata aatttggttg tgaaaaaatt tttactgatc atatgagtgg
      301 ggcaaaaagt aatcgaccag gattagaaat ggccattgat tttgttcgct ctggcgatac
      361 acttgtcgtt tggcgcttag atcgtttagg tagaaatatg gaagatttga tttcaatcgt
      421 caatcgactg aatgagcgtg gtgtgagttt tcatagtatt caagaaaata ttacgatgga
      481 caaatcaagt tctacaggac agctcatgtt tcatttgttt gcggcatttg cagaatttga
      541 gcgtaatttg atcctggaac gatccgcagc aggacgagca gctgctcgtg caagaggacg
      601 gtttggtggt cgtcctgaaa aattgtctga acaagatttg gaattactga agactttagt
      661 ggatagcggt acacctatta agactattgc ggaacggtgg aacgtctcaa gaaccaccat
      721 ttatcgttat ttggcaaaaa tggaggagaa aaatggaaag acaaattctc attagttttt
      781 gtcatttaat gctgaaaatt tttgccattt atctagaaaa gtgacaatag tatgagaatt
      841 ttttgtaagt gtctactgat aacttattat caaatgttgt aaggatttta ttcatttgtt
      901 aatattaaag aacggaactc caaaaaagtc cgtactctaa tttgcagtct ctcactcttt
      961 tagagtgaga gtttttatta gattttcaaa ttaattagta aagtgataga ataaattgat
     1021 catacaaatc ataagaagaa gcagggtgat agcataccta tgcttctttt tttatgtatt
     1081 tagctatttc ttctttacaa catcgaacat atgtttgtat aatgtggaag aatggagtgt
     1141 tatttatgat ggattattca aaagagccag taaatgatta ctttttaatc gatatgaaaa
     1201 gcttttacgc aagtgtcgaa tgtatagaaa gaaatttaga tccattaaca acagaacttg
     1261 ttgttatgag tcgatctgat aatactggtt caggattgat attagcttct tctcctgaag
     1321 caaaaaagcg gtatggtatt acaaatgtga gtagaccacg tgacttacca caaccatttc
     1381 ctaaaacact acacgttgtt ccaccacgta tgaacctata tatcaagcga aatatgcagg
     1441 taaataatat tttcagaaga tatgtggctg atgaagatct actgatttac tcgatcgatg
     1501 aatcgatttt aaaagtgaca aaatcactga atctttttac gactgaagaa acacgaagtc
     1561 agcgaaggaa gaagctcgct caaatgatcc aagaacgtat taaagaagag ttaggtttga
     1621 ttgctacggt aggcgtcggc gataatcccc ttttggctaa attagcctta gacaatgaag
     1681 caaagcataa tgaaggattt attgcggaat ggacgtacga aaacgttcct gagaaagtat
     1741 ggaatattcc cgaaatgacg gacttttggg gaattggttc tcgaatgaaa aaaaggctca
     1801 accaaatggg aattttgagc attagagacc ttgctaactg gaatccttat acgattaaga
     1861 atagattagg tgtgattgga ttacagctgt acttccatgc taacggaatc gatcgaacag
     1921 atatagcaat cccgccagaa ccgacaaagg aaaaatctta tggtaattca caagtgttac
     1981 cacgagatta tactagaaga aatgaaatcg agctggtggt aaaagaaatg gcggaacaag
     2041 tagccattcg tatcaggcag cataattgta aaacaggatg tgttcattta aacattggta
     2101 cgtcgatctt agaaacgaga ccaggatttt cacaccagat gaaaatacct atcacagata
     2161 atacaaagga attgcagaat tattgtcttt tcttgtttga taagtattat gaaggacaag
     2221 aagtaaggca cgttggaata acctatagca agcttgtgta caccgattcg cttcagctgg
     2281 atttatttag tgatccacaa aagcaaatta acgaagaaaa tttagataaa attattgaca
     2341 agattcggca aaaatatggt tttacgtcga ttgtacatgc ttcttcaatg ttagaaagcg
     2401 ctagaagcat tactagaagc acacttgttg gtggtcatgc gggcggaaat gggggaatta
     2461 aaaatgattg attacaatga tcgtggacgt ttaaagtggg cagggtttta tttatctgat
     2521 catactgaag aaattgatgc cgacaatgaa caacgaacca gtcaaaattt agcaaaagaa
     2581 caaatgacga ctgaagagat tgcggaagta ttgaacgctg cagtcataaa aaatagatca
     2641 gtatctattc aaaaagaaga acgaaacgct gaaggatact atccagaaga tattgttggc
     2701 aagatcttag gtagtgatga gctggggatc tacgttggaa aagacaaaat tcactacgac
     2761 gaaatacgtc atatcgaatt ttaccaggat aataaatgga gtcgattaga atgaacatta
     2821 tccagcaata tgagctaaag tatataacct ttgatcagct atcagaagag atttggggtt
     2881 atggccaact tatctctatg aatccttttt tgtttttaca aataggagta gatactagaa
     2941 taatgcgtgt gttaaactat tattgcaaac gcaataataa aagaaagtag gcttacgcac
     3001 catgtctatc aatacatttg tttactcgca cccgatcaat gtttacatta tcaagaattt
     3061 aggaatcact gtagaacaat tttgtgagtt atatgcctac ccacaaggaa cggttgcaag
     3121 ctggataacc cgacaacgta gaataaaatc attacctgct tcatttgtat atgatctaag
     3181 tcttgcttct agtatgaata tgaccgatgt atacgaaaag ttattaatac tagaaaatga
     3241 atatgatcaa ttcaattcaa atcagcagcg aaaagtaaag aaacagatag actagaatag
     3301 ctattttcga aaagcaatat ttaaggtata agttatacct taaataata
//
LOCUS       DACECZ010000171         3337 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.178, whole genome shotgun sequence.
ACCESSION   DACECZ010000171 DACECZ010000000
VERSION     DACECZ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.178"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..781)
                     /locus_tag="IWO35_002608"
     CDS             complement(<1..781)
                     /locus_tag="IWO35_002608"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0317628.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEIL"
     gene            complement(784..2415)
                     /locus_tag="IWO35_002609"
     CDS             complement(784..2415)
                     /locus_tag="IWO35_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ0317629.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            2732..>3337
                     /locus_tag="IWO35_002610"
     CDS             2732..>3337
                     /locus_tag="IWO35_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0317630.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHL"
BASE COUNT         1070 a          639 c          483 g         1145 t
ORIGIN      
        1 atagaatttc atagacatca ttatctggtc gttctgcaat acttccagta cataacgtaa
       61 taccattaga aggagaatcc actacattac acaaccattc taaatcgtca cgatttttaa
      121 caatacgagg taatccgaaa atagaatatg gtggatcatc tggatgaact gccattttaa
      181 tcccaacttc ttcacatgtc gggataattg ctttgagaaa atatgcaaag ttctctcgca
      241 atttcgcttc atcaacttct ttataggctt ctaataattg cttgatttgt gataaccgat
      301 gaggttccca acccggcaat tcaaaattat tagtagaatt ttctactttt ctaataattt
      361 cctctggatc agtaggaata ttttttgaaa tataggctaa cgtagaagac ccatcatcta
      421 aatgataatc caaatctgat tttacccaat caaagactgg cataaaatta taacagataa
      481 cctcaatccc aaattctgcc aaattgcgaa tcgtctgttt ataattctcg atgtattgat
      541 ctcgtgttgg caaaccaatt ttgatatcat catgaacatt aacactctca ataactttca
      601 aggtaaggcc attggcttcg acttcatctt ttaaagcttt aattcgttct ctcggccaga
      661 cctctccggc tggtatatcc attaatgttc caacaattcc tttcattgct gggatctgac
      721 gaatttcttg tagtgagatt ttttcttcat ttgccccata ccatctaaaa gtcatttcca
      781 tagttacttt cctccaactt ctgttatata tttattgatt gttttacgaa ctgcttgagg
      841 acctactagc atttcgcaaa agattttttc aattttcttt cctatctcaa cttgatataa
      901 atctaaacca aatatttcct tattttctaa gatttcctgt accgcacaat gaatctttgc
      961 aggatcttgt tcacctaatt tcaatactga gaatttttcc tgcaactcta ccaataaagg
     1021 atcaggactt ggcacaaaat tttttccttg atcatcgact gccaataaat aacgtaacca
     1081 agcagcaata gcgataggaa taaatgttag attttctgca tttccgtctt tttcgacata
     1141 ctttttaatt gtttcaccat agcgaatcgc tactttttga gaagtatctg ttgcaattcg
     1201 ttgcggtgta tctggaatat agggattagg caaacgttta ttgattactt catcaataaa
     1261 ttgttttgga tcaataacgc ctggactttc aactactggt aagccttcta cataacctat
     1321 ccctttaatt aagccaacca aatcatcatc ttgcatttct gcctgaattg atttataccc
     1381 caacaaacaa cctgttattg ctaatgccgt atggagcgga tttaaacatg tcgtaacctt
     1441 catgatatct gctttatcaa ctgtttctct atctgtcata atcacaccag ccgcttccag
     1501 tttaggtcga ccatttggaa aactatcttc aatgactaga taatggactt tttcagtatt
     1561 ggtaaaagca gcgatattgg tatttttcgc agtatgaatt atatctaagt cttcgaaacc
     1621 cgccttcaat aaatccattt tgacactttc cgaaggattt ggcgtaattc tatcaatcat
     1681 actccaagga aaagtcactt tttctttatc ctttaaataa gcaataaatt tttcagaaac
     1741 gtatcctttt ttttgccaag cttctgcaat cataaaaatg ctatcttgaa aacgttgacc
     1801 attctgtgag aaattatcag tagaaaccat cgcaatcggt aactctccag cattaaacct
     1861 gctatacaac aaggatgtca caatactcat cgtatgttga ggattggtag gtccagattt
     1921 tatatctgct tccactaccg aaaaaaattg tcctttagaa tcttttaaac tgtatccttt
     1981 ttcagtaata gtaaacgttg taaactgtaa tgtaggatct tcaaaatatt taaccactct
     2041 ttgatagtcc gttatattct ttggattgca atataaacta tcagctgtta ctgctaaaag
     2101 tctttttttg agttcaccat cttcacccat aacaatctcc aaaatatcat tgttgtagtc
     2161 atgataagct ttttctacta cttcttcatc aaatgtctca cacacaacta ctccggattc
     2221 taattcattt tgatctgcta agcattgagc aatttctgca tgaaatcctc gataaagatt
     2281 tcctccacca aaatgaagcc acactggatg tttctgacct tcttgtttga ccgcttcgac
     2341 attaaaatac ggtagttgaa cttgcatttt gttcaatcct tcgatagaat cttgccagtc
     2401 tttaatcaaa accatattta cccctccata ctgatatgtt agtttccgtt acaattgtag
     2461 tataaaatcg aatgtaatcg ctgtcaataa gaaaaaacta aaaatattct tttttctacc
     2521 atgtcagaat tattgtgtct ttttttagta aaagcacacc aatatatatt ttttagaact
     2581 aacttaaatt aaagcttaat ttctctttga tcttatttga gtattttttg tttttttaat
     2641 agctagtatg ttactttgaa atttgatatt atataacgag catagaacta aaatatcaaa
     2701 ttcaaaaatg aaattttgag agggattaaa tatgacaatt aaaactagtc tccaaaatca
     2761 agcttatcaa tctattcgtc agcaaattat ctacgctgac ttagaacctg gaaaaaaagt
     2821 atcagaaaaa aaattggaag aaacgctttc tatcggtcgt actccaatac gtgaagcatt
     2881 gattcaactc cgtcatcaag gacttgttga tacaattcct caaagtggaa attatatctc
     2941 attaatcgac ttagatttag caaaaaatgc tcgttttgtc agagaacatt tagaacgaca
     3001 aattatgcta gagtgttgtg ccaagatgaa taatcaaaag aaacaagttt tagaaactat
     3061 aattgcagaa caagaaaaag ctgttagtca aaagaatgct cgtagttttt ttcataatga
     3121 taatttattt cataaagctt gttttgaaat tgcagaacgg gaagaagtat ggcaatggtt
     3181 ggaagataat aatacgcact tggaacgatt tcgttggtta cgagtaaaaa cggaaggatt
     3241 aaaatgggaa aaaattatgg aacaacatca tcaactattt aaagccttga ccatgcaaga
     3301 ccctgaagaa gctaacttct taacatcctt gcatctt
//
LOCUS       DACECZ010000172         3335 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.179, whole genome shotgun sequence.
ACCESSION   DACECZ010000172 DACECZ010000000
VERSION     DACECZ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.179"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(451..1761)
                     /locus_tag="IWO35_002611"
     CDS             complement(451..1761)
                     /locus_tag="IWO35_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0317631.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="IWO35_002612"
     CDS             complement(1773..3191)
                     /locus_tag="IWO35_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0317632.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT         1032 a          676 c          515 g         1112 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataag
//
LOCUS       DACECZ010000173         3264 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.180, whole genome shotgun sequence.
ACCESSION   DACECZ010000173 DACECZ010000000
VERSION     DACECZ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.180"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            8..2161
                     /locus_tag="IWO35_002613"
     CDS             8..2161
                     /locus_tag="IWO35_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ0317633.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2347..3156)
                     /locus_tag="IWO35_002614"
     CDS             complement(2347..3156)
                     /locus_tag="IWO35_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0317634.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         1072 a          602 c          683 g          907 t
ORIGIN      
        1 ataggaaatg atgaaagatt ttatgaaaaa aatattaaat acttcttggt tagaaaaaat
       61 aagaaaaggt gcacaagaac ctaagaacaa aacgtttcgc cggtcccatg ttccttttcg
      121 cttaaatttt ctttttttca ttatatttac gttatttgtt gcgttgattg ctagattggg
      181 ttatttgcag atcgtgaacg gcgaagagat ggaggcgcga gtgaagtcta cttctacgat
      241 tacggtccaa gaaagtactc ctcgcggaat gatctacgac tccacaggaa aagctttagt
      301 tacgaataaa gcgaatcagg ccatcacata tacgagagga acacaagtta cggctaaaga
      361 tttgcttgaa atcgcaacga agctgaactc gctgatcgat gttccggtag atgaaaatct
      421 gacagataga gataaaaaag atttttggct ggctgatccc aaacatttga aagaagccac
      481 aaaaagactt tcggcaaaag aaaagcaatt agggaacagc gaacaatata gtgcgatggt
      541 caataaagtc acagctgatg agattaattt aaacgaggag caattaaaaa ttgctaccat
      601 ctttaaacgg atgaatagtg catatgaatt aaacactgtc tatatcaaaa actctggtgt
      661 tactgatcaa gagttagctg ttgtggcaga acgcgcttct gaacttccag gagtttcgac
      721 tggcacagac tggacacgag aatataatgc agcaagttca ctgaaaagta ttcttggcag
      781 tgtgacgact gaaaagcaag gacttccagc agatgaagca gagaaatacc tagcaaaagg
      841 ttattctcgg aatgaccgtg taggacaaag ttatttggaa aaacaatatg aagatgtcct
      901 gcaaggaacc aaaacccaat atgaagtttc ccttgataat gaaggaaatg tttcgaatca
      961 aaaagaaatc ttctctgggg aaaaaggttc taatttaatg ttatctatga atgcagaatt
     1021 ccaatcaaaa gtagaagaaa ttttgaaaag gaattatcaa acattgatca acaacggcaa
     1081 agcacagtat tcgcctggtg cttatgcagt ggctatgaat cctcagacag gagaagtgct
     1141 agcaatgact ggtttcagtc atgaacaagg ttccaaagaa atcaccgaga atgctttagg
     1201 cacgattact agcgcctttg atccagggtc tgtagtcaag gctggtactt tgacagcagg
     1261 ctgggcaagt aacgcgatca gcggaaatca agttttgatt gatgagccga ttcgtctgca
     1321 aggtgcttca gaaaaaagct ctgtctttaa tcgaagcgga caagttgctc ttgatgcagt
     1381 caaagcatcg gaattgtctt ctaatacgta tatgatcaaa gtggcgttaa agatgttagg
     1441 actagactat acacctggaa tgggactacc tagtttagat gaagaagcta aagcttatca
     1501 gcaattgaga gactctttca aagagtttgg tcttggtaca acgactggga tcgatttgcc
     1561 gaatgaatct cctgggatct cacgttctgt tgattatatg aagaagttca atgcggataa
     1621 tggcaaagaa tggtatgctc caggtaactt taccgatttg gcatttggac agtttgacac
     1681 atatacacca atccaattag ctcaatatgc ttcaacagtc gctaatggcg ggaaacgagt
     1741 acagccgcac ctagtcaaag cgatctatgg caatgacgaa aatggaaact tgggtgaagt
     1801 gaagaaagaa attggcacaa cagtagaaaa tacggtcaat atttctgcag aaaatatggc
     1861 gatcctccgt gaaggattcc accaagttgt ccatggtacc gatccttaca caacagccaa
     1921 accattagcc agtgcgaaaa tggacctttc cgcaaaaaca gggactgcag aaacggttgc
     1981 ggaaggtcat ccagatattg cgacagtcaa tagtaatatc gtggcatacg gaccaacaga
     2041 caatccggaa attgccatca gtgttgtctt gccgaactta ttagacgaac aagaccacat
     2101 aaacttgacg attgctaaag aaataatgga cgcgtattat gatatgttca tggcaaaata
     2161 aaaaagaggt cgtgaaaaag ccgcttagtt tcgagtatga agaaaaattt tgtgaaaata
     2221 gcttctcaca tttttgctta aatttgactt cataccgaga aactggcttt tgaacaccgt
     2281 ttactcggca aaaaaggttg tgacaaaaat tgtcacaacc tcttttttgt ggaatctaat
     2341 tctctattat agtttaagta gtttttccag ataaacagct aaaccgtctt cctgattgct
     2401 caaaggagtg acatcatttg caatgccttt gagttggtca gtaccgttag ccatagctac
     2461 tccccaacct gcataatcca acaattccaa atcattatgc tcgtcaccaa aggcaatgac
     2521 gtctttttga tcgatagaca ggtagttagc aatctcttgc acgccttttg ctttttggat
     2581 tccttttgaa acaatctcta agatggcagt aggtccaccc catgtccgaa catcgacata
     2641 actgccaaat tgacgtgtta attcagcaga tacagctcct gcaaaacgtt tgtctgttcg
     2701 gacaagcaaa gatgtaggat ttgtcgtcaa atttttagga gaaagcaaat tcttctctcc
     2761 tggacgagaa gaagcaaaaa ttttttcatc aaagaaatca aaactgtcaa taaaaaaagt
     2821 atcacgattt tcagcagcga taaaatctaa atttagctgc tttttttgcg ataagatttc
     2881 aaaagcgatt tcacgattga tcaacgtttc tctttcacca tcccagtgct gattagggat
     2941 gtgtactaat gcgccgttaa aattaaccat cggtgttgtt aattctaatt gttggtaaaa
     3001 ctgatggctc attcggaatg ggcgtcctgt agcaatactg acatgatgac cggcattgat
     3061 tgcttttttt agagtctctt ttgttttaga tgtaatcatc gaatcttgat tgagggtagt
     3121 tccgtccaga tcgatggcaa ttaatttttt attcaataag ggtccttcct tcctcaacta
     3181 gcatttgttt tagtctagca tattttaggt aattaacaag agcaatcagc tattcctaac
     3241 aaaaactaca gttatggcta tata
//
LOCUS       DACECZ010000174         3108 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.181, whole genome shotgun sequence.
ACCESSION   DACECZ010000174 DACECZ010000000
VERSION     DACECZ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.181"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            59..907
                     /locus_tag="IWO35_002615"
     CDS             59..907
                     /locus_tag="IWO35_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0317635.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            1007..1441
                     /locus_tag="IWO35_002616"
     CDS             1007..1441
                     /locus_tag="IWO35_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0317636.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            1621..2031
                     /locus_tag="IWO35_002617"
     CDS             1621..2031
                     /locus_tag="IWO35_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0317637.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            2088..2528
                     /locus_tag="IWO35_002618"
     CDS             2088..2528
                     /locus_tag="IWO35_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0317638.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            2631..>3108
                     /locus_tag="IWO35_002619"
     CDS             2631..>3108
                     /locus_tag="IWO35_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0317639.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGK"
BASE COUNT          955 a          510 c          593 g         1050 t
ORIGIN      
        1 gtgaaaagta agttataata agataagagt attattaatg aattaaggaa gtgaataaat
       61 gaaagaaacg agacaattta tatggacata cattaagatt atttttgccc tcttcatatt
      121 aggagtcgct atcaatatgt ttttagggcc tcatcatatt gctgctggag gtgtcagcgg
      181 tctaggaata ttactggaag ctgctttagg ctttgaccga gctatagtta ttttagtgtt
      241 gaatatcgtt atgttggtac tagctcttct atttttaggt aaaaaaccct tttttaaagt
      301 acttttcgga agcttggttt ttcctttaat cattgcaata gttccagaaa caatgattac
      361 ttcagatcgg ttgttgtctg tcatttttgg aagtgcgatt tttgctttag gagttgcgat
      421 tttatataaa aacaactctt ctagcggcgg tacgacgatc cctcccctca tttttaaaaa
      481 atatttccac ttgaatactt cgattggctt attttttaca gacgcattcg ttgtttctct
      541 taatctgttt atttttggaa tagaagagtt tctttatgct attttatcta ttgcaatcac
      601 ttcaattgtc atgacttata tcgaaacagg tatcaatcga aagaaatctg ttatggtcat
      661 gagtgaacat tatttggaag acatccgcct tcgcttaagt gctgagattg gacgtggcct
      721 taccctgcta gaggcaaaag gcgggtataa tcgaaagtca aaagaggttt tattgatcgt
      781 tgtgactgat catgaatttt cccgaatgaa accattgata gaagaaatcg atccaagtgc
      841 ctttgtcatt gtcagcagcg tagcagaagt aatgggacgc ggatttactt atcatccaat
      901 cgaatgagtt ggtaagtaaa tccgctattt ttttgaatgc gttttcttta gctggtataa
      961 taaaagaatc tagtaactgt tcgactgaaa aggagaggag atctttatgc ataaaggaac
     1021 ggaaatcgat tttgtattaa atgatagttt gaaagcacta gaactgtatc aaacaatctt
     1081 tgatgaaata caactgattg aggcaacaga ttaccctaga ggcaataatg aagtcatttt
     1141 taccttgtat ggtacgagat tccatatgct ggatgaaaac acgtctcttg gtttgactgc
     1201 tcctactgct gaacatccaa atacgatctg gttcaatatc atcgtctctg atatcgagga
     1261 cacacataat aaagcattac gaaatggctg tgtagagatt caagaaatga cagaaatgat
     1321 ggactttggt gtgactaact caatgttcag agatcctttt ggttatttat ggatgcttca
     1381 tcaggtccat cgtgaagtaa tttttgaaga aagaaaacaa ctttttgaga aatatatgta
     1441 atgtttatga tgagattatt ttttgtctct gatatttttt gtatatgttt gctattcgag
     1501 aaagcgcgtc tgtttcatgt gctttctctt atttatctta tgccaatcca tttctttgct
     1561 ttccacaatt atattgtgta aaattcaatt gtataaaata taattaaagg tggtaatttt
     1621 atgaaaaaaa tgtattctac gaaaatgatc aacacaggcg gacgctctgg agaagtgcat
     1681 aatcccgatc gtacattcga attgaatatt gcagctcctg gtaaacgcgt cgaaaatgca
     1741 accaaccctg aacaattgtt tgcagctggt ttcagtgcat gttttaacag tgcattagaa
     1801 ttgatcaaac agcaaaaaaa tatctctggg gcttctactg tcagtgcaca agtttccctt
     1861 tattcagaaa gtgaaacgag ctttgtctta ggtgtagaga ttgaaggatc tatcgaaggt
     1921 ctttctctgg aagaaacgca agaattatta gaggcagcgc ataaagtgtg tccgtattca
     1981 aaagctacag ctggaaatat agaagttact ctgaaagcag ttagtgaata gacttttttg
     2041 tgtaaatccg ctatgataca ctatagtaaa acagcggagg tggaaaaatg gaagatctat
     2101 atttagttaa tcaagtatgt tttcctttat atgccgcctc taaagaaatc attagacact
     2161 acactcccct cctcaagccg ctgaatctta cctataccca gtatcttgtg atgttagttt
     2221 tatgggaaaa gaaaaagact tcagtgaaag agctaggaaa tgatttgtgt ctggattctg
     2281 gtacgctgac tcccttacta aaaaagttag cagaaaaaaa ttatattaat cgtcagcgtg
     2341 aaacgggtga tgaaagaatc gtaaatgtta cgctgactgc tcaaggtgct gcgttgcaaa
     2401 aacaagcaga aggtattcca caaaagatca agtcttatct tccacttgaa caggaagaat
     2461 tggatacact gcaacatctg acccgaaaaa tgctagataa ttttcatcaa gaccagaaaa
     2521 agggctgaaa ctgctagatg aacgatttta tgaggaaaac caactcataa gataacgtca
     2581 tttagcggtt tttctttttg ctctttctta ttttcatatt ccatcatata atgaataacg
     2641 tgtttatttt ttgttatttg gaggttgtcg atttgaccgt ttatcagcgt tttttggcga
     2701 atgaaaaaat acgccgtctc actgttttat tatttgtcgt ttttgttctt ttcttagtta
     2761 gaagtatgat tacaaccatt ttgctcacat ttatctttac ttatttgatg attcatcttg
     2821 ttcaaatcat tcagcgattt gtccgaatcc ctacaggtgt tttagctatc atgatttata
     2881 cactgatcgt ttaccttatc tatctggcat tcacaaagta tatcccgatg cttattcatc
     2941 aaacatttga catgatccag tccgttatta atttttacga gcatcagcca aaagatgcag
     3001 atcctgtttt acaatatatc catacttata ttgagcgaaa cgattttttt gaacagcttc
     3061 aaaatggcgc ttcgattgcg cttggctatt tacaggatat aggaaaaa
//
LOCUS       DACECZ010000175         3107 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.182, whole genome shotgun sequence.
ACCESSION   DACECZ010000175 DACECZ010000000
VERSION     DACECZ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.182"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            77..832
                     /gene="fabI"
                     /locus_tag="IWO35_002620"
     CDS             77..832
                     /gene="fabI"
                     /locus_tag="IWO35_002620"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ0317640.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(942..1955)
                     /locus_tag="IWO35_002621"
     CDS             complement(942..1955)
                     /locus_tag="IWO35_002621"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ0317641.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            2120..3040
                     /locus_tag="IWO35_002622"
     CDS             2120..3040
                     /locus_tag="IWO35_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ0317642.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          904 a          625 c          592 g          986 t
ORIGIN      
        1 caagaagtta ttttagctat tattctctat ttttttacga ctatcaaatt tttatcagaa
       61 acaaaggaga gtcattatgt catttctaga aggaaaaaaa atcgtcatta tgggcgtcgc
      121 taacaaaaaa agcattgctt ggggttgtgc tgaagcaatg ttgaaacaag gggcagaaat
      181 tatctatact taccaaaatg aacgaatgaa aaaatcatta gtcaaattgg tcggcgaaga
      241 agcattcact gtagaatgtg atgtttctag cgacgaaagc atcgaaaaag caatgaatgc
      301 catcggtgaa tatgctgggg agatccacgg ccttgtccat gcagttgctt acgccaacaa
      361 agaagaattg tccggaaatg tgtctgatat ttccagagac ggctatcaat tagctcaaga
      421 tatcagcagc tactctttaa ttgctgtatc aaaatacgca caaccgtttc ttgcaccaaa
      481 cagcgggatc gttagcatga cttatctagg ttcagaacgc gcaatcccga attataacat
      541 gatgggtatt gcgaaagctt ctcttgaagc tgctatccgt tatttggcag ctgagttctc
      601 gcctaaagga attcgggtca atggaatctc agctggggca atcaaaacat tagctgtcac
      661 aggtgttaaa gactatcaaa aactgatcca gttatctgaa tctcgtacgc ctgatcagca
      721 aggtgtgacg atcgaagaag tcggtaatgc ttgtgctttc ttaatcagcc cactttccag
      781 cggaatcatc ggtgatatta tctacgttga caaaggtgtc catctttcat aaacttttgt
      841 tgcattcttt aaagtaaaac atataaaaat caggaaaaaa ctttttaaag tctcttcctg
      901 attttttctt tttacgtttt tttcaactca ttactttgaa attattccac gacttcaatc
      961 cagccttctg gtgcttctat gtctccaaat tgaatacctg tcaattcatc atacaaccgt
     1021 tttgttactg gtcctacttc cgtttcacta taaaatacat gaagattgtt attgtgataa
     1081 atcccaccga ttggagaaat aacagccgct gtaccacatg caccagcttc tgcaaattga
     1141 tctagatcat caatgaaaat atctccttca attgcttgca gtcctagtcg ttcttcagct
     1201 aactgcaaca aggaatattt cgtgatactt ggtaagatag agggcgattg cggagtgatg
     1261 aattttccgt cttttgtaat tccaaagaaa tttgctgcac ctacttcttc gattttcgta
     1321 tgtgtggctg gatcaagata aacacaatct gagtaatcca gttcttttgc ttctgttcct
     1381 ggaagtaaac tcgctgcgta atttccgccg acttttgctg caccagtccc cttaggagca
     1441 gcacgatcgt aatcagatac aataaaattc gttggtttta gtccaccttt gaaatacgct
     1501 cctactggcg tacagaagac gacaaataaa tattctggag ctggatggac accaatgttt
     1561 tcaccgaccc caatcagtaa agggcgcaag tataatgtag cgcctgttcc atagggcggc
     1621 acaaaatctt catttgcttt taccacttgc ttgacagcat caacgaacat cttttcaggg
     1681 accattggca tcagcaatcg tctagcactt tgattcatcc gtcgactatt ttgttcaggt
     1741 cgaaagagat tgaccttgcc gtcttttcga cgataagctt ttagaccttc aaaacattgc
     1801 tgaccataat gaagcgctgg agagccttca tgaatcgtca ggtaattatc ttctgtcaat
     1861 tctccttctt cccattgtcc gtctttccat ttggcaataa aacgccaagg agttttgata
     1921 taagaaaaac ctagattgtt ccaatcgatt tgcatatact taccaccttc gttattttct
     1981 ccacatttta tcattatcca tattatttgt cattttattt tcagcttttt tccttcatta
     2041 tagttatttt tgctatttcc ttttttgaaa acttcttggt acaatacaga aagaatcaaa
     2101 ttttagaaaa gaggatttta tgttttcatt attagcaact gccggtacga cagactcttc
     2161 tggcgagaca ttgacagatc aagctgtcag caaagtaaat gcgtttcagc ggttttggga
     2221 cgggattgat tgggacgcca ttttggctgc ctttatccaa aaaagtttat atctgatttt
     2281 tttgatcgtc cttttttgga ttcttcaccg tgtgggaaaa tatctgattg ataagtcttt
     2341 taatcagtat tctaaaaaag ccattctaaa cgagagccgc ttaaagactt tacatagtct
     2401 tttaaatact atttttcatt atacccttgg tttctttttt atctatgcaa ttttatctgc
     2461 tatcggtgtg ccgatcggat cacttttggc tggtgccgga attgcaggtg tggcaatcgg
     2521 tcttggtgcc caagggttca tgagtgatgt tattaccgga ttttttatta tcatggaaca
     2581 acagatggat gttggggact acatcaagct agcaaactta acgattgaag gaaccgtcgt
     2641 atctgttggt atccgtaccc ttcagctgaa agcaacggat ggtactgtcc atttcatccc
     2701 gaatcgaaac atcaccacga tcagcaatct ctctcgtgca aatatgcaag tacttttaga
     2761 tattcggatt gttcctgaag aaggatatga caaaatctat gagatcattg atcgagtaaa
     2821 tcgtacatta gctgaaaaat accaagaaga gctccaaact gaaccttcta tctttggatt
     2881 agtggatctt ggaaacagca attttgcgat tcgtacggtc tgctacgcat tgaacggaaa
     2941 acaatatgcc ttaaaagaag agttcttgag ctcctatgtc aaagaattga cagcttctgg
     3001 tttcacgatt ccgaacagtc caattgctgt cggaaaataa tttggacaaa cgatgcaacg
     3061 aatctcacta aaaagagcgt gggacaaaag taaagaacac ttttgtc
//
LOCUS       DACECZ010000176         3101 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.183, whole genome shotgun sequence.
ACCESSION   DACECZ010000176 DACECZ010000000
VERSION     DACECZ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.183"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(7..978)
                     /locus_tag="IWO35_002623"
     CDS             complement(7..978)
                     /locus_tag="IWO35_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ0317643.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            1683..3062
                     /gene="brnQ"
                     /locus_tag="IWO35_002624"
     CDS             1683..3062
                     /gene="brnQ"
                     /locus_tag="IWO35_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0317644.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT          860 a          617 c          588 g         1036 t
ORIGIN      
        1 caaagcttat tcttgatgga gatctgcctt ttcataagct ttgatgattt cggcaactac
       61 agggtgccga acaacgtcac ttgcttcaaa attaacaaaa tcgattttct tgatttgctt
      121 taatgttcgt tctgcatgaa caagaccact tgttgtccct cgaggcagat caatctgact
      181 cgtatctcca ttgacaatca tttttgattg ataaccaaga cgtgtcagga acattttcat
      241 ctgagcaact gtcgtgttct gtgcttcatc caaaataacg aatgcatcat ccaatgtccg
      301 cccccgcatg taagctaaag gagcaatttc gatcacgcct cgttccatca atcggttcgt
      361 atgatctaat ccaaaaattt ggtataaagc atcataaaca ggacgcagat aaggatctac
      421 tttttctttt agatccccgg gaagaaagcc tagattttcc cctgcttcca cagcaggccg
      481 cgtcaaaatg attttttgca cttcaccttt tttcaaggca gcgattgcta atactactgc
      541 aagaaaagtt tttcctgtcc cggctggtcc tacgccaaat acgatatcat gtttggctac
      601 cgattcgaca tatttctttt ggccgctgtt ttttacccgt ataggttttc cattacggtc
      661 tttgacgatc tcttcctcat acatctcaac aaaataatct aatgtgcctt tttgccccat
      721 tttcagtgcc gtcacgacat ctggtgtact tacatggatt cctcgtttga tcaattcctg
      781 caatgtccga atgactgagg cagccaagga aatctgggat tgttcgccaa tgatctgaat
      841 gatttctcca cgtgtatgga tggttgtttc tgttgtttct tcaatcagtt ttatgtgctt
      901 gtcgtgtgca cctaaaagca tgctgacatc atcagcagcc gtcaatctaa tctctaaaga
      961 actagactct tccgtcaaaa atagacatcc cctttttttc gattcattct ttcctatttt
     1021 accatactca gaagaaacta tatagcaaat aagaaaaggt tctaaaagag ttgcttaacc
     1081 ataagtacca agaaacgtta aaaaatagct gcttccattc tctaaaggtc tcgtttgaat
     1141 aaaaaaagct gatcatgtaa gctatgcata agatagagga caagtattcc gtctaactta
     1201 gcaagtattc tctgggtatt tttcttgttt caaaaaaaga aaacaaaata tttgaaatat
     1261 tcttgcattt caaaaaaaac aggtgtatta taacgtcaac aaataaatat acagcaaagc
     1321 gtcgaggaaa acagtaggct attcagtgat ttattcccag tgagtcatgt aagtgagaaa
     1381 tgacaataaa tcgaatagac gaacatcatt tccgagtcaa tgtcgaaaac gtagatcatt
     1441 gtggctgcct ccattatcag gccagcggat aactttatcg aaaagcgtcc gtgaatgaag
     1501 aagctttttt aagcgaaaaa aggtggtacc gttctagaat ctagagccct tttcccaagt
     1561 ggaaaagggc tttttttata ttttaaggtg gtggttttca tggaatctga tattagtggt
     1621 atttctagtt tctgcaacgt ttattttgtt actcaaaatg ataattattg gaggaaaaat
     1681 ttatggaaga aacaaaaact tattcatgga agcaattatt gatggtcagt tcacttatct
     1741 tcgggatgtt cttcgggtca ggtaatctga tctttcctgt ccacttaggt caaatggctg
     1801 gatcaaactg gatcagcgct ggtgtaggat ttgccatctc aggcgctctt ttcccattat
     1861 tagctatctt agctgtagta gtgacaaaaa gtgatggtct gtatgactta gctaaacctg
     1921 tcggaaaatg gtatgctgcc ttgttcttga tccttgttca tttaacgatc ggaccattct
     1981 ttggtacacc gcggacagca gcaactgctt ttgaaatggc tgctaaaccc tttttgcctg
     2041 aaaaatatga ttcattaggt atgtttttat tctctgctct tttcttttta cttgcttatt
     2101 tccttactgt acatccaaca cgtttggtca aatatgtcgg aaagtattta aactccttgt
     2161 ttcttgcatt attagctatc atttttgtcg tttctttatt ccaacccatg gggaatctaa
     2221 accaatctgc tgcagattct tatcagacga acgctttgac aaatggcgtt ttagaaggat
     2281 acaacacggt tgatgcagta gctttacttg ctttaagtgt cacttttgtc catgcagttc
     2341 gtgatcttgg ttttaaagat aaaaaagtca cagaattaac agcaaaagct ggtagcctga
     2401 gcgttgcgtt agaagtcatg atttattttg gtctggtcct tctcggtgcg atgagtttga
     2461 ataagatgtc tttatcagaa aatggcggaa ctgcactttc tcagatcact aatttttatt
     2521 taggtaactt cggtgctagc ttcttagggg taatggtcac attaggtgtc ttcactaccg
     2581 ctatgggact cgttgtttct tttgcacaag atttccataa actgttccct aaagtcagtt
     2641 atatgacatg gctgcggtta acgacttttg tctcttttgt cgttgcgaat gccggattgg
     2701 ataatatcat ccaatggtct ttaccagtat tgatgctttt gtatccattg tcacttgcgt
     2761 taatcttgct ttccttaaca gcaaaattct tccaaaagag tccatttgtc tatcaagtaa
     2821 cgatgttctt ttctgccgtt ccagccgtat tggatatgtt ggcaaattcg cctgcattag
     2881 tgagtcagca aactgtcgta gcaagcttac taggatttta tcatcattat gttccttttg
     2941 ctgcattagg acttggctgg gtcgtcccaa cgttgcttgg ttacgctggc agcttgctct
     3001 tttattatgt atatcgcgta tcaggatata aacaagaagc aagtgagtta ccagaagaat
     3061 aaccaaatca aacacaattt aacataagag atattaaaca g
//
LOCUS       DACECZ010000177         3094 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.184, whole genome shotgun sequence.
ACCESSION   DACECZ010000177 DACECZ010000000
VERSION     DACECZ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.184"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(311..925)
                     /locus_tag="IWO35_002625"
     CDS             complement(311..925)
                     /locus_tag="IWO35_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0317645.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1531..1908)
                     /locus_tag="IWO35_002626"
     CDS             complement(1531..1908)
                     /locus_tag="IWO35_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317646.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2208..3089)
                     /gene="galU"
                     /locus_tag="IWO35_002627"
     CDS             complement(2208..3089)
                     /gene="galU"
                     /locus_tag="IWO35_002627"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0317647.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          935 a          648 c          478 g         1033 t
ORIGIN      
        1 tttcatcaac gtgtattaag agacaagaaa gaatccatat ggattctttc ttgtctcttt
       61 ttccactact ttttattttt ctgacactac atattcttat aactcatttc tcataactgt
      121 gaaacaactt ataaaaataa caaactttaa acaaaacaag cttaaaatca aagatgatcc
      181 atcttaaaat aactaaaaca aaaaaagtag tcgttgttaa tcctcattaa taatcgtttt
      241 ctttttttca aacgctccat ttttatgtgg aacattattt ttttaaggaa ctttgtcaac
      301 atgtggataa ctattttcca cttgtgtcct ggactagctg ttttgctgta aaatacaaaa
      361 cttgctgcag caaaaagatc gttgcaagag aggctgttcc gcagatcgct ggatctagat
      421 ttggcaaagt caagactgca tcatgtaaat tggcgtggtt gtccaaaacc aaatcagcaa
      481 attcaaataa tcgcaaacca ctgctatgtt gcgatttgat acttcgtgac aaatcaaatc
      541 cagtaacaat gattaatgga tgaccatttt ctttgatcca ctgagctagt tcgatgattg
      601 ccgggtttct tccttcattc gaaagaagaa gaaaaatgtc ttctgatttg acagaaagct
      661 gatcaataat tgcctgtcct gcaccttcta gcgtttcata gacacctccg gctggatttt
      721 caatcaatat acttggtaca agatccgggg tttgatccca aaattcaaat gctacaccac
      781 ttagtgtgcg gctagcatat atctgcaatc taccaccttg attaatttgt ttcgcgatcc
      841 aagtgccagc ttgaagcatc agcggctctt ctatccttaa tttctcttcc aattggtgat
      901 aatagtcaaa ataacataaa ctcatttatc tctcccgcct agctatactt gacatgaata
      961 gaaacagctg cctaatctaa ggaagtacat aaaaaaacag tagttagaca gtaacacatg
     1021 tggtctttgc tgtttcaaac acaaaaaagg cattgaacga atacgcccaa taccttgaca
     1081 tagcaaataa aacaacgtgc agaccatgga aaatatctac ataggggtag aattttcacc
     1141 ctagattagg tacaataaat taggtcgatg cactgcattg actatggcaa aatgatcggt
     1201 cagggtgtat gtgtgtgact ggtcattccc tgattcttag cgccaactaa gggtcaggga
     1261 gccttttttt attcagctgt ttctatctga atttattgta agtaatctct tatccaagtg
     1321 caagcaagaa aatacaaaaa aacattggta tgatttatat atatataaat aacataaaaa
     1381 aaggaatggc ttatcccgat acaacaagca ttcctttaaa aaagattctt ttatataagt
     1441 gaaaaaagct tttttccacc aattaaataa atctctcatc tcaatagttt atccattatt
     1501 ttatcttttt ttgcgtatac ctactaaata ttaccgtctt cttctcgttc ttctactttg
     1561 ttcgagagaa tctgtactag aaaaagtagt tgtatgtcta ccttttctgc ctatccgaga
     1621 ctttcttttc atatatggga tataaatgat tagatacagt acagcaaaaa ataaagcaaa
     1681 tacgcaagct tcccaactac ctaacgccaa ttcaaccatc aaggtagcaa ccccaacgga
     1741 tgctgcatac cagataaaaa tcagaaacgt ttcttttttt ctgaccatgc gaaacatgat
     1801 aaatagacta ataagcaata gtggcaacac aaatatccaa ttgcttcctt ccatcagcca
     1861 cttgagtata tcaagcagcg actgtgcaac agcgactaat tttcccatag tgaacacaca
     1921 ccttttttta taattaaaaa attcgccttt ttattctact atgaatcttc tggcttggct
     1981 ttcatttctc ttatgtaatc attttctttt atacaactta aaaaaaaatt aaaccattag
     2041 cagaatattt tctgactcta ttcacgaaag tctttttcta tctttcgttt tccacgactc
     2101 aactgatcta gagtcaaaaa actaaaacga cagttgcgca aatgacgcac aagctgccgc
     2161 tttaataaca aatagccttt cattctcagc aggccactta tttccagtta actttctgtt
     2221 cttagctttt tactcaactg gataatatat gcacttaatg aagtcccaat ttccgaatgt
     2281 tgaagtccat attcaatatt tgtcttcata tatcctaact tatcgcctac gtcgtatctt
     2341 tttcctttaa actctctcgc atagaccgcc tctgtctggt taagagtctg aagtgcatcc
     2401 gttagctgta tctcgtttcc tgctcctggt tcttgagttt ctaatatctc aaatatttta
     2461 ggagtcagta gataacgtcc gataatggct aaattacttg gtgcttttcc catttcaggt
     2521 ttttctacaa acccttttac tctgcaacga cccttttcgc tatctgtttt aggatcaata
     2581 attccgtatt tagatgtttc attttctggt acacgcatga cggcaacagc ggaagcttgt
     2641 gtttcttcat aatccatcat caattgttga gtcaatggga tatcatcttt catcaaatca
     2701 tcccccagca tgacgacaaa cggttcattt cctataaatg cttttgcttg aagcacggca
     2761 tcccccagtc ctcttggatg agattgacgg atgaagtgaa gattgacttg agtcgtttct
     2821 tcaactaaag cgagtaattc tgttttttcc ttttcccgca aattgttttc caattcgata
     2881 tttgcatcaa agtgatcttc aatgggtctt ttttcttttc ccgttacgat caagatgtct
     2941 tcgattcccg aatccaatgc ttcttccaca ataaactgga tcgttggttt atccacgatt
     3001 ggaagcattt cttttgccat ggcttttgtt gccggcaaaa aacgtgttcc tagcccagcg
     3061 gcaggaatga ctgctttttt gaccttcact tgct
//
LOCUS       DACECZ010000178         3064 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.185, whole genome shotgun sequence.
ACCESSION   DACECZ010000178 DACECZ010000000
VERSION     DACECZ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.185"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(52..987)
                     /gene="murB"
                     /locus_tag="IWO35_002628"
     CDS             complement(52..987)
                     /gene="murB"
                     /locus_tag="IWO35_002628"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ0317648.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1060..1980)
                     /locus_tag="IWO35_002629"
     CDS             complement(1060..1980)
                     /locus_tag="IWO35_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0317649.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2093..2845
                     /gene="xth"
                     /locus_tag="IWO35_002630"
     CDS             2093..2845
                     /gene="xth"
                     /locus_tag="IWO35_002630"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ0317650.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          859 a          622 c          533 g         1050 t
ORIGIN      
        1 gcaggacggc caccgtcctg ctcttttact tattttaagt tccattactt attaattggc
       61 taaacttctg cttgtttctg atattgcttg tttttctcca atgatccgca cttctgtttc
      121 taaagacaca tcaaattttt ctttgattac ttcttggatg tgcgcaatca attcaatata
      181 atctgtcgct gtcgcatgat cgatattgac gatgaatcca gcatgttttt cagagacttg
      241 tgcaccgccc catttcaggc cttgcaatcc tgcttgttgg atcagctgac ctgtatagtg
      301 tcctactggg cgcttaaaga cacttccgca tgaagggtat tctaatggct gttttgattg
      361 acgcaattcg gtcaactcat ccattcttgc tttgattgct tcataatccc ctgtttgcaa
      421 actgaatcga gcttccaata cgatagcacg taattcttgg actttactgt gacgataaga
      481 aaataccatt tcgtcttttg tcaaagtttt caatgttcca tcttcaagca aaagatccac
      541 ttcagcaaaa acatctttga tctctccgcc atacgcacct gcattcatat aaaccgctcc
      601 accgacactt cccgggatgc cgcaagcaaa ttcaaatcca gtcaatgatt ctgccaaagc
      661 tgcataagtc gtatcaatca gtgaggcacc tgcttccgca atgaccatcg ttcctttgac
      721 atgaatctgt ttcatttcag tcagcatgat gactacaccg cgaatcccgc catcttgaac
      781 gatcagattg cttgcatttc ctaataccat ccaaggtata tcatggattc ggcaataatc
      841 gatgatttgc tttacttctt cttgtttttt cgggaatgca aggacatcag ctggtccacc
      901 tgttttcgta aacgtatagt tcatcaatgg ttcatcaaac aatatagtaa tcgtggctaa
      961 gctttcaact atttcttctt tattcactga ggattttcct ttcttttctc aaacgtcctc
     1021 attattctat cacgcttaat acaatcttgc cagtcttgtt caaagttgat gacggtagtg
     1081 atctttaagc acttcctcac ataattcatg acttcttaca acttcttttt gtttaagcaa
     1141 ttctgtttcc gatttaccta aacggatctg agaaaagaag gcctctacca ttggctcaaa
     1201 cccgcgtttg actagcgtgt ttgtccagtc gccaaattct ttcacttttt ttccatcagc
     1261 atcttgaatg acaagctggg tcagatcgtt tacttcataa gtaccatttt tacttgttac
     1321 ttgatatttt tctatattag cgccacttgc taaatccatc gttaaaatag ccgtttgatt
     1381 ttgtgtttct aattgtaaaa tggcatattc caaaaattcc tttgtttctc ttatctggta
     1441 ttttgttctc aaaatgggtt catctaataa atacacagct gtatcaataa gatgtaaaaa
     1501 caaatcataa aggacgaact ctgtcgtttc ttttgccgct attcgatttt tttgcaggtg
     1561 gatcaaccgc ttatctggga tacgtttcaa ttcttctacc ataggagcaa agcgccggtt
     1621 gaaaccgatc atcaaaatta tttggttctt atcagctaat tcttgcaatt cacgaacttc
     1681 atttagatgg acactcagtg gtttgtcaat ataaacatgg ataccatttt ctagacattt
     1741 tttggctaat tcataatgta cttttgttgc tgcgtggatc atacatgctt cgattttttc
     1801 ttctaaaagc tcatcgatcg tttcatatag atgttggaac ccgtactctt tttgaattct
     1861 ttcttttgtt tctttatttc ttgttgcaaa ataaaaatca agtgttcctt gttttttcgc
     1921 ataagttggt aaatatgctt tttctgcgat attccctaca ccgattacac caatcttcat
     1981 ctactttcct cctggatcat ttagttactt tcatcttaca ccatttttta ctaaaatgag
     2041 gtacaatagt atcgcaaagg taaggaaatt aattcgaagg agtgtgcttt tattgaaatt
     2101 tatctcttgg aacgtaaacg ggcttcgtgc catcgtaaat aaaaattttt tagaagtttt
     2161 tcatgagtta gacgccgatt ttttctgtct gcaggaaacg aaactccaag caggacaaat
     2221 tgatttagat cttccaggtt attatcaata ttggaattac gcggaacgaa aaggctattc
     2281 cggcacagcg atttttgcta aaaaaccagc attgaatgcc acttacggaa tgggtatcga
     2341 tatccatgat acagaaggcc gtctgattac tttggaatat tccgatttct ttcttgtaac
     2401 ctgttacacg ccaaattctc aaagcgaatt aaaacgttta gattaccgat tagaatggga
     2461 agaagccttt tataattatt tggaaaatct aaaaaaacaa aaaccagtca tcgtgtgtgg
     2521 ggatctaaat gtcgctcatc aaaagattga tttgaaaaac tggaaaacga atcaaaaaaa
     2581 tgctggattc actccagaag aaagagcggc tctttctcga ctgttggata acgggtttat
     2641 cgatacattc cgttattttt atccaaccca ggaaggtgtc tactcttggt ggaactaccg
     2701 attcaattca aggaaaaaca atgctggttg gcggatcgat tactttttga cgagcaaaga
     2761 tttagagcca ggactagctg atgcaaagat ccatacggac attatgggca gtgatcattg
     2821 tccagtagaa ttagatttga aataaagagg ttgtgaaaaa gtcgtttagt ttcgagtatt
     2881 aagaaaaatt ttgtgaaaat ggctttccac attttgcata aatttggcat aataccgaga
     2941 aactggcttt tgaacactgt ttatcgagag attgtgaaaa agctgtcctg catcaagtaa
     3001 taagaacagc caaacaaaaa tagttcctca tatttttcgt ttggttgggc ttattaccgc
     3061 agat
//
LOCUS       DACECZ010000179         3032 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.186, whole genome shotgun sequence.
ACCESSION   DACECZ010000179 DACECZ010000000
VERSION     DACECZ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.186"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..849
                     /locus_tag="IWO35_002631"
     CDS             <1..849
                     /locus_tag="IWO35_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ0317651.1"
                     /translation="LISVLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFL
                     VLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDR
                     KVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETD
                     FYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIF
                     SLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYL
                     YLKKEN"
     gene            851..1921
                     /locus_tag="IWO35_002632"
     CDS             851..1921
                     /locus_tag="IWO35_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317652.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            1908..>3032
                     /locus_tag="IWO35_002633"
     CDS             1908..>3032
                     /locus_tag="IWO35_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ0317653.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILL"
BASE COUNT         1023 a          371 c          487 g         1151 t
ORIGIN      
        1 ttaattagtg tattaatttc aaaaataaca aaaacgattc gtgtttttta tatgatgaat
       61 tttatcgtac aaatatttta tattacactt tcttcgtcta gaggtgccca ggtagtaatt
      121 tgttcttttt tagtattatt tcttttgttg tttgccaatt caaaatttag aaagagcatt
      181 agagaagtac tacctgtaaa aatcaaatgg atggtaagca tattattagt tctattggta
      241 tatttttctt ttggtactaa ttatgctaag gattttttag caacaattcc tgtatcgatt
      301 gatagaaaag tagaagttat agagtcaaat gaatcagata aaactccact tccagaaaaa
      361 gaagtaatat tacagcattc tagtgataac tccgaaattt cagctggtcg ttttacgatt
      421 tggaaagctg cgcttaaagt tataagacca actttgttat ttggttatgg agagaccgat
      481 ttctatggga atagcgatgc aaaatttttg aaaaaagtag atcttgatac tacagatgtg
      541 aatgaactaa aaagagcaca tggaaacatg cataatggat ttcttcaagt acttgtatca
      601 agtggtataa ttgcttttat atgtatctat gctttctatg tgttaaatat ctttagttta
      661 ctcaaaacat tttttagaag taaagtgaat tatgcgtttc ttggtattgt tttaattttt
      721 atactatcta tttttataga tgaaatggtg gaggctcatg tacttttcaa taagcgcgat
      781 gtcatttcaa ttgttttctg gtattatttg ggaagcattt cttacttgta tttaaaaaag
      841 gagaattaat atgaagacag ctttcattgc ttggactcct ttacatttga ttaatatatt
      901 aaatgtttat caaaattttt accgagaatc aaaaggagat ttatttattt attctgaatt
      961 tgaaaacgct agaagatata gtgagcgtat aaaagaatta gggctattta atgatgttgt
     1021 ttttgtgaat caccggaaaa tcggttcttc ctttaaacgt aaaattaact tgttaactaa
     1081 ccataatcaa tttttctcgg atgatttatc cgtttatgat gaaattttta tacaggggga
     1141 aaattatttc acaaaaatac ttttctccaa tgtttacaaa aaaaatccag atgtaaaact
     1201 aaattatata gaagatggtt tgggtactta tttgaagaag agtatatttt caacaagtaa
     1261 aaacgttcaa agagttttta aaatattaaa taaaaatagc atcttcaaaa aaaaaattga
     1321 cgattatttt gtctattgtc cggaattgtt actttatgat gtaaaaaata taaaaaaaat
     1381 tcccaaaata gatgaaaaca gtaaattgat gaaaaacata caatcaatct ttgggataga
     1441 agaagttttt gtcggagata aatgtatctt ttttgatcaa cctttattat ctgatggtta
     1501 tggcatagat gaaacagata ttttcgctaa agtaaatagt atattttctg gaaaaaaaga
     1561 aactttaatt aagcttcatc ctagagcaag gacaaatcgt tatggtgaaa aaaacatatt
     1621 gaattccagt ttaccgtttg aagtcttgct acttgaaaat gacttctctt caacactttt
     1681 gattagtcct atatctacaa tttctttttc accatctatg atgtttgaaa agacgatcaa
     1741 ctctattatg ttggctaaat taattttaaa tgaatataca gcattatctg aagataaaaa
     1801 agaaacattg acaaatattg ttcttttctg tgaacattat aaccgtctat cagaagaaaa
     1861 aattttactc ccagaaaact gggcagaatt aagaaagttg gtagagtatg ggattattta
     1921 acacactttc caagtcaaaa ttagttaagt ccagtttctg gtatactata gggaattttt
     1981 ttattaaggg aatttcattc ataactattc ctatttttgt aaatattact actcaagcag
     2041 aatatggttt gattaataat ttcacttcat ttgtatcaat tttttcgatt cttgttgggc
     2101 taagcctaaa ttcggcaatt aataatgcaa gttttgacta taaagatgac ataaatgaat
     2161 ttatgtcatc ggtactttct ctagctacac tttcgttaat tgtgttctta ttaataggga
     2221 acatatattt tttgttttca aatgcttttt ttgatatgaa ccaacttgta tttaatttga
     2281 tgttattaca aagttttgct agttttgtaa ttagttatat ccagtcttat tttacgatta
     2341 actttaacca ttttaaatat ttgattttat cttttatatc tacttttttg aatattgcaa
     2401 tctctgtagc tttgatgcag acattatatt ttgatgataa atatgttggc agagtagtcg
     2461 gcggaacact gtcatttgtg ttgataggtt tagtaattta tttaagaata atgataaagg
     2521 gtgctcattt ttttgataaa aaacagtgga gatacgcttt aaaaatctct ttgccgctaa
     2581 tcccacattc cttagcaaat atagttttag cacaattcga tagaattatg attaattctt
     2641 atttaggtcc tgctgcgggg ggaatatatt cctacattag taacataggt attattctaa
     2701 gcgtaatatg gatgtctact aataacgcct gggttccatg gttctatggt gagatgagta
     2761 aaaataatga aaaaaaagtg aagaaagtta gtaactatta tttagtagtc tttactttga
     2821 ttacgatgat tgtaatggta gtttcgattg atttagcaaa atttatggct ccgaaagact
     2881 attattctgg gttatcttta gtggttccga taaccttagg ctatttcttt cagtttttat
     2941 atagtttacc agttaatgct gaattttatg agaaaaaaac atcgtttatt gcgattggta
     3001 cagtagcaag tgctatagtt aatatactat ta
//
LOCUS       DACECZ010000180         3024 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.187, whole genome shotgun sequence.
ACCESSION   DACECZ010000180 DACECZ010000000
VERSION     DACECZ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.187"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(287..649)
                     /locus_tag="IWO35_002634"
     CDS             complement(287..649)
                     /locus_tag="IWO35_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317654.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="IWO35_002635"
     CDS             complement(723..1103)
                     /locus_tag="IWO35_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ0317655.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="IWO35_002636"
     CDS             1185..2360
                     /locus_tag="IWO35_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0317656.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="IWO35_002637"
     CDS             complement(2437..3021)
                     /locus_tag="IWO35_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0317657.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          942 a          578 c          498 g         1006 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tatt
//
LOCUS       DACECZ010000181         2931 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.188, whole genome shotgun sequence.
ACCESSION   DACECZ010000181 DACECZ010000000
VERSION     DACECZ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.188"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            108..545
                     /locus_tag="IWO35_002638"
                     /pseudo
     CDS             108..545
                     /locus_tag="IWO35_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(622..2169)
                     /locus_tag="IWO35_002639"
     CDS             complement(622..2169)
                     /locus_tag="IWO35_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ0317658.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(2271..2624)
                     /gene="tnpB"
                     /locus_tag="IWO35_002640"
     CDS             complement(2271..2624)
                     /gene="tnpB"
                     /locus_tag="IWO35_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ0317659.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2614..2808)
                     /locus_tag="IWO35_002641"
     CDS             complement(2614..2808)
                     /locus_tag="IWO35_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317660.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          865 a          600 c          537 g          929 t
ORIGIN      
        1 tatataaggt ataccctaac tagacacgac cacaacattg aaaaatctgg ttttatcgtg
       61 tatgattagg gtgtatttta aatagttgca gaaagtaggt aaaaattttg agagttgcat
      121 acatacgtgt ctcatccatc gatcaaaatg aacaacgcca gattgaagaa atgaaaaaat
      181 tcggtgcagt gagaattttc atcgagaaac aatcgggcgc aacgattact catcgacctg
      241 ttttccaaga agcattggat ttcgtccgag atcaagacat ttttattgtg gaagctattg
      301 atcgacttgg aagaaattac gacgagatca tcgcttcagt caattaccta aagaagaaaa
      361 acgtacagct aatcatcacc agtctaccga tcatggctga agcaattggc aaccctctcc
      421 tggataaatt cattaaggat ctgatcatcc aaattttggc catgatcgct gaacaagagc
      481 gtaccgaatc taaacgacgc caggcgcaag gcattaaaat tgcgaaagca aatggagttt
      541 ataaagtaag cgccccatag aacagtacct gtacgtcaaa taacccgacc tatagaatag
      601 aatcgggaat gtttggcgtt tttagttacg agaaaaatgt tgttgtacat ccttgttcca
      661 aggcaacagt tgatctaaag tttctgctga aagtacggga agatttggta tcttatcaaa
      721 tagaaactct agatacttta caggattcaa tccgttaagt tctgctgttt tatatacact
      781 cagaatgatt cctgaagttc gtgctccttc aagacttgtg gaatgcaacc aatttttccg
      841 tcctatcacc agctctttga tatttcgctc cgcacgatta tttgtgagtt ctaaacgacc
      901 atcttcaaaa ataggttcaa aaccacttcg tagtttacac gcatattgaa cggctgcatc
      961 caacttggat tttgcaacag gatctatcgt tgccatccac tcataaaaag caataaataa
     1021 tggacgcagt tcttgttgcc gacagcttaa tcgcttctca ggagaaaagt ctttccattt
     1081 cttttctagg acaaaccatt tatccaattg tttgactgct tgagctgcgg gaatatccgt
     1141 gtttttcttt ccttttggaa ttgcttcgaa aaatttccgc cgaacatgcg cgaaacaata
     1201 gacaagtcgt acactttcca cggcattata cccgctatag ccgtcacagt gtaaataccc
     1261 tgtaaactcc ttgagaaaag ttttcggaac atctccagca cgactttcgt cgtggtgata
     1321 ataaacgatg ggatgctgac tgtcttttcc tgtacaaaac tgccagtaat aggtatctgt
     1381 tttggcactt tcaatcacac gataacttgt ttcatccccg tgtagaatct cttcttgatt
     1441 aagatagact cttaatcgtt ctcctaaagg gtctaacgcg ttttgcactg ctttaatatg
     1501 ccaattggtg atattatctc tcgaaatatc caacccatgt gtttgtttcc aatagttctc
     1561 ttgccgatag gcaggaactt tctgaccaaa tttcatccgg atcgtttccg ccactaaact
     1621 attggaacct aagctattag aaatcaaagg tttaggaacg atcgcttttt taaaagaaga
     1681 aagtccttct ttttcgcagc ttttacaaca ataggtatgt tgataatggg tgagacaatc
     1741 caacatagcc tgatggaaac gaatttcttc tcgtactttt gtccgtccga tatctttcat
     1801 ctgtgaaccg caatgttcac aaaattgttc ttccaaagga aggagatgtt ctttctctgt
     1861 tataggtaag tgtttgattt tgtctgcttt cgttcctaca actcgttgat gtccttttac
     1921 tggaataatg gtggcagctg cttctggaac acttacagaa atttcttcat cactaaaaag
     1981 attcaattgc ccattcgttt cctcaaagag agtctcttta gagcgtccaa ataatttgtt
     2041 ggttaaataa ttgacctttt cattcaatag ttggatttca gttgtaagat tctgaatggt
     2101 ttgttgttgt gcttgattgg ttttatgggc ttcttgaagc aactgaagca acgtatctgt
     2161 agtttccaaa atttttcacc accttgaatc gaactgattt tgttacagaa taccacaaga
     2221 aacgcacaag aaataggggt tcaacacatt ttttgtgtta accccccagc ttaaaaaact
     2281 cctttttttc ctggcaaaat agctcgaggt tgatcgatac ttaatccttc taacaaccat
     2341 cgaacctgtt ggttcgttaa ggttcgaact tcgtctttcg tccgaggcca ttttaacttg
     2401 ccgttttcta tccgcttata acagagaaga aaaccatcac cgtcccaata gagaagtttg
     2461 aaacggtctt gacggttccc gcaaaataaa aaaacggcat cttcgtaaag ctcgagttga
     2521 tactcttgtt gaataagact ggcaagacca tcaattcctt ttcgcaaatc tgtttttccg
     2581 cacacaatat aaatatgttt ggtttgaagt aaatcataaa gcattggaca acagttcttt
     2641 caaaatagct tgaagaatat aattgttaat accgttatag atcaaaactt cagaaccatt
     2701 ctctcttttg atgcgcatcg ctaacttggc agccgcgggt ttaccttttt ctggtgcact
     2761 cttttcgttt cctaattgga caggaacaat ctcatttaat ttatacatag aaaacctcca
     2821 gacttcttga tagctcaagt atatcttgag gtgcatttgt tagtaaggta ctgttctatg
     2881 gggcgcttac tttataaagg tcgtccaaaa ctctatagtg cagatacaaa a
//
LOCUS       DACECZ010000182         2793 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.190, whole genome shotgun sequence.
ACCESSION   DACECZ010000182 DACECZ010000000
VERSION     DACECZ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.190"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1012
                     /locus_tag="IWO35_002642"
     CDS             <1..1012
                     /locus_tag="IWO35_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HAQ0317661.1"
                     /translation="DLKDEDLLPATLSKELITDLLRGKLGFNGLVVTDASHMLGMTGA
                     MKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKGIISEERLRDALRRILGLKAK
                     LGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQAITLVKDKQDIWPIVPEKYKR
                     ILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFEVEKYISPEEELKGLSQEEKI
                     KNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIVWPASKGTPDIPFYIHEIPTI
                     FVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLMGLSEFKGISPIDAYCGLKDT
                     FI"
     gene            1040..>2793
                     /gene="uidA"
                     /locus_tag="IWO35_002643"
     CDS             1040..>2793
                     /gene="uidA"
                     /locus_tag="IWO35_002643"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAQ0317662.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIA"
BASE COUNT         1039 a          345 c          568 g          841 t
ORIGIN      
        1 tgatctgaaa gatgaagatt tacttcctgc aactttatca aaagaattaa tcactgactt
       61 acttagagga aaactcgggt ttaatggatt agtagttact gatgctagtc atatgttagg
      121 aatgactggg gcaatgaaac ggtcagaatt attgcccact tctattgcgg ctggatgtga
      181 tctattccta ttttttaatg atccagatga agactttaat tatatgatgg aaggttatta
      241 caaaggaatt atttcagaag agcgtttacg tgatgcatta agaagaattt taggcttaaa
      301 ggctaaacta ggtttgcata aaaaagaaaa gaataaaatt ttgttaccga aacaagaagc
      361 tatggaaaaa attggtttaa ttgaaaataa atcaattttt aaagaggtag ctgatcaagc
      421 aattacacta gttaaagata aacaagatat ttggcctatt gtaccagaaa aatataaaag
      481 gattttactg gtagatgtac atggggtaaa aggtgggttt ggtgaagtag ttgctggaaa
      541 tagacctcag gccattgatt tattgaaaca gattttgtca gcaaaaggat ttgaagttga
      601 aaaatacatt tcacctgaag aagaattgaa aggattatcg caagaagaaa aaattaaaaa
      661 tatagctgga atatatgctg ctaagagacc aatcaaagat ttaactgaaa aatatgactt
      721 gattattaat atagctaatg ttaacccagg tacagttcaa agaattgttt ggccagcaag
      781 taaaggtact ccggatattc cattttatat tcatgaaatt ccaacaattt ttgtatctgt
      841 acagtgtccg tttcatttag ctgatgtgcc tcaagtaaaa acttatatca atacttatga
      901 tggaaaacaa gaaactttag aaatattagt ggacaaacta atgggccttt ctgaatttaa
      961 gggaataagt ccaatagatg cttattgtgg gttaaaggat acatttattt aatatttaaa
     1021 ttatatagga gtgatttaca tgttatatcc cattatgaca gagacgaggc aagtaattga
     1081 tttaagcggt ctttggagat ttaagttaga tacaaatgat gacttacaag aagaacttgc
     1141 tttagaacca atggttgaaa aagagagtta tccaatttca gttccttctt cttataatga
     1201 tttatttgaa acagagaaca ttagaaatca tgttggatgg gtgtggtatg aaaaagaagt
     1261 gattcttcct aaaaaattat tagaagaaag attagttctt cgttttggat ctgcaacaca
     1321 tgaagcaaaa gtatttttga atggaaaatt gattaatcaa cacaaaggag ggtttacgcc
     1381 atttgaatca gaaattaatc agtttataaa agatggaagt aacagaatta cagtggctgt
     1441 aaacaatgtt attgacgata gcactttacc tgtagggatt ttgaaagaaa cgatacagga
     1501 agatggagac ataaaaaaag aaaatttggt taattttgac tttttcaatt atgcaggcat
     1561 taatagaccc gttaaattgt atacaactcc gaaagactat ataaaggatg tcactattaa
     1621 tacaaaaata attgattcag atgcaaatgt agaatttaat atcgatacag aatggaccgg
     1681 agaaattcta atagcaatct atgacgaaga agctcaactt atagcagaag ctaaggggaa
     1741 agaaggagtg attcaactta agaacccaac actttgggag cctctaaatg cttacttata
     1801 tagtatgaaa gttagtttaa ttaacgaaaa taaagtgatt gatacgtaca cagaagaatt
     1861 tggcattcgt agtattcagg tttcgaatgg gaagtttttg attaataata aaccttttta
     1921 ctttaaggga tttgggaagc atgaggacac gtatattaat ggtcgtggtt tgaatgaagc
     1981 agtaaatatc aaagatttta atttaatgaa atggatcggt gctaattcat ttaggacttc
     2041 acattatcct tattcagaag aaattatgcg cttagcagat cgagaaggta ttgtagttat
     2101 tgatgaagta cctgcagtag gattacattt gaatttcatg gctactttat tagatgacaa
     2161 cgtagaaaaa catgatactt ggaaagaaat acggacagaa aatgctcaca aaatggcgat
     2221 taaggaattg atagaacgcg acaagaacca tccgagtgtc gtaatgtggt ctattgcaaa
     2281 tgaaccagat tcggattctg aaggcgctaa agaatatttt gaacctttag ttgaattgac
     2341 taaagaactt gaccctcaga agagacctgt aacaatcgtt acttacttaa agtcaacacc
     2401 agatatttgt aaagtaggag atattgtcga tgttttatgt ttaaatcgct attatggttg
     2461 gtatgtttca ggtggagacc taaaacatgc aaaatatctg ttgaaaaaag agctagatgg
     2521 atggatggaa agatgtcccg atacgccaat aatgtttaca gaatatggag cagatacagt
     2581 agctggactt catgatacag taccaacaat gtttacagaa gagtatcaag tgggttatta
     2641 cgaagcaaat cacgaagtaa tagataaatg tgacaatttt gttggtgaac aagtgtggaa
     2701 ttttgcagat tttgaaacaa gtcaagggat tttaagagtg caaggaaata agaaaggaat
     2761 ctttactaga gatagaaagc ctaaaatgat tgc
//
LOCUS       DACECZ010000183         2780 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.191, whole genome shotgun sequence.
ACCESSION   DACECZ010000183 DACECZ010000000
VERSION     DACECZ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.191"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..197
                     /locus_tag="IWO35_002644"
     CDS             <1..197
                     /locus_tag="IWO35_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317663.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IWO35_002645"
     CDS             314..811
                     /locus_tag="IWO35_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0317664.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            786..1292
                     /locus_tag="IWO35_002646"
     CDS             786..1292
                     /locus_tag="IWO35_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0317665.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            1276..>2780
                     /locus_tag="IWO35_002647"
     CDS             1276..>2780
                     /locus_tag="IWO35_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ0317666.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT          839 a          515 c          600 g          826 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gactacgagt taccctttac
      481 ggttgacgaa tacacttcca ttggcgaact caatcgacta tgggaaatgg tatcggaatt
      541 acccgaagaa ttacaatcgg agctatctgc tctgctcact catttttcaa gcattgaaga
      601 actaagcgaa catcaagagg atattatcat tcattccgat tgtgatgata tgtatgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtaccagcta gtcttcaaaa
      721 ctatattgat tatcaagcct atggtcggga tttagacctt tcaggaacgt ttatctcaac
      781 caatcatggg atttttgaaa tcgtctatta aatctgtcgg tacattacta ctggcagatt
      841 ttctatttta cggggtggct caatcagcta cccctatttt ttatgaaagg attgattaca
      901 tgaagaaaat acgaagctat accagtatct ggtctgtgga aaaggtactg tattctatca
      961 atgattttag acttccgttt cccataacct ttacgcaaat gacatggttt gtcgtgtcac
     1021 tctttgcagt gatgatactt ggcaacttgc cccctctttc catgatagag ggagcatttc
     1081 tcaaatactt tgggattcct gtggctttca catggtttat gtctacaaaa acttttgatg
     1141 gtaaaaagcc ttatggattt ttgaagtctg tcattgctta tgcactgcga ccaaagctga
     1201 cctatgcagg aaaaaaagta acgcttggca gaaaccagcc acaagaagcc attacagcag
     1261 ttaggagtga attttatggc atatccaatt aaatacattg aaaacaatct cgtctggaat
     1321 aaagacgggg aatgttatgc ttactatgag cttgttcctt acaattactc atttctaagt
     1381 ccagaacaga aaatacaagt gcatgattct ttcagacagc ttatcgcaca aaatcgtgat
     1441 ggcaaaattc atgctttaca aatcagtaca gaatccagca tacgttctgc acaagagcgt
     1501 tccaaaaatg aagtcactgg caagctcaaa gcggttgcct atgacaaaat cgaccaacag
     1561 acagacgctt taatatccat gattggcgaa aatcaagtga actaccgttt ctttatcggc
     1621 tttaagttgc ttctcaacga tcaggagttt tctatgaaaa gtcttaccgt tgaagcaaaa
     1681 aatgctttgt ctgattttgt ctatgatgtg aaccataagc tgatgggcga ttttgttagt
     1741 atgagtaatg atgaaatcct gcgttttcag aagatggaaa agctcttaga aaataaaatc
     1801 tctcgtcgtt tcaaaatccg caggttagat aaggacgact tcggctatct gattgaacac
     1861 ctttacggac agacaggcac tgcctatgaa gagtatgagt accatctatc aaagaaaaag
     1921 ctggataatg aaacgctgat taaatactat gacttgatta agcctactcg ctgtttggtg
     1981 gaagaaaaac agcgatattt gaaaatccag caggaagatg aaaccgtcta tgtagcttac
     2041 tttaccatta acagcattgt cggagaactg gacttcccgt cctctgaaat cttctactac
     2101 cagcaacagc aatttacatt cccgattgat acgtcaatga atgtggaaat tgtagcgaat
     2161 cgtaaagccc tatctactgt ccgcaataaa aagaaagaac tgaaagactt ggataaccac
     2221 gcttggcaaa gtgataatga aaccagctcc aatgtggcgg aagctctgga aagtgtgaat
     2281 gagctggaaa ccaatttaga ccaaagcaag gaatctatgt acaagctgtc ttatgtggta
     2341 agggtatcag caaatgatct tgacgaactc aaacgtcgtt gtaatgaagt gaaagatttt
     2401 tatgacgatt taagcgtaaa actggtacga ccatttgggg atatgctcgg cttacatgaa
     2461 gaatttttac ctgccagcaa gcgttatatg aatgattata ttcaatacgt gacctctgat
     2521 ttcctcgctg gtttaggttt tggtgctact caaatgctgg gggaaaatga ggggatttat
     2581 gttggctaca gcttagatac tggacgcaat gtctatctga aacctgctct tgccagtcaa
     2641 ggggttaagg gttcagtaac caatgcgtta gcgtcggctt ttgttggttc gctgggtggt
     2701 ggtaaatcct ttgcgaataa ccttatcgtc tattatgcgg tgctttatgg ggcacaagca
     2761 gtgattgtag acccaaaagc
//
LOCUS       DACECZ010000184         2744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.193, whole genome shotgun sequence.
ACCESSION   DACECZ010000184 DACECZ010000000
VERSION     DACECZ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.193"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            142..1209
                     /gene="rlmN"
                     /locus_tag="IWO35_002648"
     CDS             142..1209
                     /gene="rlmN"
                     /locus_tag="IWO35_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ0317667.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(1402..2505)
                     /locus_tag="IWO35_002649"
     CDS             complement(1402..2505)
                     /locus_tag="IWO35_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0317668.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT          866 a          491 c          551 g          836 t
ORIGIN      
        1 ttttctaata cggtatgatg tcttgtcttt ttagtttttg tgagattaaa gttctattcg
       61 atttatttgt gaagtattgc attaaggaaa tgaatcatgt atagtatgta gcgaaacttt
      121 gagaaaaaga gggaaaatac tttggaaaaa cagtccattt atggcttgac gaatgaagaa
      181 ttaatcaatt ggtttataga aaacggcgag aaaaaattcc gagctgccca agtttgggaa
      241 tggttgtatc aaaaacgtgt aagtaatttc acagaaatga cgaatctatc gaaacaattg
      301 atcgaaaaat tatcggagca ttttatcatc aatcctctaa aacagatggt cgtccaagaa
      361 gcttcggatg gtacagtaaa atatttgttt gaactccctg ataaaaacat gattgagact
      421 gtactgatga gacaagaata tggcttgtca gtttgtgtga cgacacaagt aggatgtaat
      481 atcggctgta cgttttgtgc aagcgggctg ctcaaaaaga accgtgatct gactgctgga
      541 gaaattgttg cacagatcat gatggtccaa cattattttg atgagcgcaa tcttggcgaa
      601 cgcgtttcac atgtcgttgt aatggggatc ggtgagccat ttgataatta cgacaatgta
      661 atggactttt tgcatatcat caatgatgcc aaaggattgg caattggtgc tcggcacatt
      721 acagtttcca ctagtggatt agcacacaaa atcaaggaat tcgctaataa cggcttgcaa
      781 gtcaatctgg ctatttcctt gcacgcgccg aataatgagg tgcgaacatc gatcatgcgg
      841 atcaaccgaa gtttcccaat tgagaaattg atggaagctg tggatgagta tttagagaaa
      901 acaaaccgtc gaatcacgtt tgaatatatc atgttgaatc aagtaaatga tagaccagag
      961 catgcgcaac aattagcaga tctattaaaa gataagaaaa aattagcata tgtcaacttg
     1021 attccatata atccagtcag tgaacatgat caatattctc gcagtccaaa agctgatgta
     1081 ttgagatttt atgatgtatt gaagaaaaac ggcgtaaact gtgtgatacg taaagaacat
     1141 ggtacagata ttgatgcagc ttgcggacaa ttgcgaagca agcaaatgaa aaaaacagtt
     1201 gccaaataat cgtatcctac tcgttttttc aattaccata aaaggagcag ttttcttaat
     1261 taaggaactg ttcaacttaa ataagagaaa agattctata gcgaaagcta taaagctttt
     1321 tttgacaaga aaaaccctca tcaagcaaag aaatgtcctc aatttgatga cagcctgctt
     1381 gatgggggtt ttagcaatta tttaactgat tttcttatat ccttgctggc tttcttgtgt
     1441 agcatgataa gtttctacgt caagaatggc ctgtttaaat ttttctcggg tgatttcata
     1501 aggcacatgt ttaatatcat ttgttgccat ggctttatct aagattgctt ctaaatcggt
     1561 cgtgacattg agattcatgt cggttaacga tactggaagt tggttctttt tcataaacac
     1621 atagatttta tcaaacaaat cgaactgctg atccatcatc aataaggtca gtacaccata
     1681 actgacagat gctccgtgaa ggtatccctc cattttttct agaacagtcg taccgtaata
     1741 gaatgagtgt gccatagcag aattatagtt attgttaact aaaacagaaa ccaatccggt
     1801 agtaacaatg atatccgaaa cgacttgttc aattgcatat gagggacgat cgtcggcact
     1861 atctttcaat gcttgcgtac cataagtcaa gagacgatca atgcacattt ttccgatttg
     1921 gattcctaat ctatccgtgt agcctagatc atcgccacga gcagaaaaag tagattcgac
     1981 ttctttggat aaagcatcgc caatacctgc ccataagtat ttatcaggtg cttcggcaat
     2041 aattgctgta tcaatgaagg tgtgtacagg tggttttgaa ggaaagaaga gtccgtccat
     2101 tacgccttct tcgttataga tgacacatac cgcagtgacg ggcgaacagt tcgaaccaat
     2161 agtgggaaag gtgaagactg gtttagctag tttttcacca atgactttta cagtatcgca
     2221 agcacggcca ccaccgactg caaacaggac gtctgctttt tggacacttt catgctggat
     2281 caactgttca atatttccat aagttgaatt acccccgtac cagagactgg cgacaacctc
     2341 tatttcctgt gtcaaagcta cttttatttt gttttctgcc tttgaaagtg cagtcttccc
     2401 tccaatcagc actacttttg tcccatattc tttgacgatt ccatttgctt tttcatacgc
     2461 atctgttcca atggtataat ttggtaagaa aacactttga ctcatattca tttccttctt
     2521 tcttcgttga aacgggttaa aattttaaag agtgatcgat ctgactgcgg ctgtagatgg
     2581 agtagaggtg aacctaagac aggataagtg atgaataagg tggcatgaac accttgaaat
     2641 catcaataag tatcctaaaa acgaacaagt acaaaaacat tgaaaacgac aataatgaat
     2701 ttttgttcct cttagtattt ctaccgattc cctttgcttt tttg
//
LOCUS       DACECZ010000185         2705 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.194, whole genome shotgun sequence.
ACCESSION   DACECZ010000185 DACECZ010000000
VERSION     DACECZ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.194"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            5..1087
                     /locus_tag="IWO35_002650"
     CDS             5..1087
                     /locus_tag="IWO35_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0317669.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="IWO35_002651"
     CDS             complement(1305..2447)
                     /locus_tag="IWO35_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0317670.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYMLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTIEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="IWO35_002652"
     CDS             complement(2452..2637)
                     /locus_tag="IWO35_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0317671.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT          900 a          489 c          430 g          886 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct gtgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caatggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacatata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatt
//
LOCUS       DACECZ010000186         2703 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.195, whole genome shotgun sequence.
ACCESSION   DACECZ010000186 DACECZ010000000
VERSION     DACECZ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.195"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            45..902
                     /locus_tag="IWO35_002653"
     CDS             45..902
                     /locus_tag="IWO35_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0317672.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            903..1451
                     /locus_tag="IWO35_002654"
     CDS             903..1451
                     /locus_tag="IWO35_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317673.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1748..2695)
                     /locus_tag="IWO35_002655"
     CDS             complement(1748..2695)
                     /locus_tag="IWO35_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAQ0317674.1"
                     /translation="MHLEFSDYESLLVKICEDYYLEDINISEISKKYGITRYKALKYI
                     EDAREKQLVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADY
                     IQELIKKANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQK
                     AAERYESSYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDS
                     VEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGI
                     CKSKFKAESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
BASE COUNT         1007 a          407 c          510 g          779 t
ORIGIN      
        1 taatagtatc aaatgaatat tgctaaaaga aaagaggtag gaacgtggta gcgaagaaat
       61 atgtaattgg taattttaaa ggtggcgtag gtaaaagtac gtgcgcgcaa atgttagggt
      121 ttgaatcagc agttaatgaa ggaaaaagaa ccttgataat tgacttggat atgcaaggaa
      181 atacttctga tgtgatgaac ttaacgcata tgaacttcac taacgaagaa ggaggcggag
      241 aaggtgaacc gattgtttac gaaaatactg tgactgacgt gttgataaac aatcttccac
      301 ctaaagaggc tgtctataaa gtaatcaata acctctatat acttccagca gatatgtcct
      361 ttgaaatgta tgatgattgg atcaaagacc aatataatga ttcattatcc caattcaaat
      421 atatgaaaga aaagctagaa ccattatttg atgagtttga cgtcatctat ttagatgtcc
      481 caccttctat ttctgtatat agtaaatccg cgatgtacat tgcggactgg gcaatcgtcg
      541 tcttacagac ccaagtaaaa tcgatgagaa atgcgatgca atatcttgag tatatggact
      601 tttttgtgaa agaatttgat acaaatttat atgttgctgg tgttattcct ttcatgttgg
      661 aaagcggtga cgcagtagat caggaaatgt ataaacaagc ccaagagatt tatggggaac
      721 atttgattaa aaatgttgta ctcaaaaatg cgcgcttaaa acgatacgac ggatcaggta
      781 ttacgatgga gaagacaaag aaaggtctct taaaacaatg ggatcgcaaa agccacgaat
      841 tatttatcag tattttgaaa gaattggaag aacacgagaa ttggtatgcg gaggtggagt
      901 aattggctga catcaaagga cttgatattt ttgggaacaa caaaggactc gccaaaataa
      961 aaaaagaagc agaagcaaag aaaaagcagc aagcaattac accatctgaa aataagcaat
     1021 tagaagtgca ccaagttcaa gaaataaaaa cttcctccaa tgttgaaaga cagagtagag
     1081 gaagaacaaa aaaagttggc gcaccaatca gaaagtttga taaggtttat tccaccaagc
     1141 atcctttaaa actgtcgact ttgttaaatg cgacttccag agttttagta gaaaagtatg
     1201 agactaatat gacaagagat gaattgctta gaaaagcttt agatgagtat attaaacaga
     1261 atcttacgca agaagataag caagatttgt ttaatgacgt ggtgaaagaa ctagacttgt
     1321 tcagagaaaa atatccaatt gttccagaat tagatgaccg tggagaaata ataagaacta
     1381 ccagtcagat aaaacaagat accgaagact cactgcgcaa agggtgggga atgaataata
     1441 aagttagata gaaccccctt tttatagcat aaaaaagatt tagaaggata tatgatccta
     1501 ttgcatgaga agtacaataa aattaataag aagtaacatt aacgaaaaaa cgtggaaatt
     1561 attcagtgaa tacaaagaaa ttaaatcttg aaaagtaatg gagtatggct tggaaaagtt
     1621 caaaaaatat caagacaagc ttctcaaaaa aagggatttt gatatccaat aaaaagagtg
     1681 aatcattgta ctgccctcca aaaaagtagt accaaaaatc taaatttttt ggaggatttt
     1741 acatgactca ctcttttttt atcaaagaaa gtattttcca agcagttttt tcatccaaaa
     1801 ataaatctgt aaaaaaatta ccattcaagg cgcctaaaca actctccgct ttgaacttac
     1861 ttttacaaat accaattcgt tttggaatat cgaaaatttc ttttaaagaa agaccaaaag
     1921 tcttatcctc agatgctttg atgaattcac cattgatatt gaagggtctt ccgtaaagaa
     1981 atccaacagt atccgtcatg cttggaaata ggattttacg atgcttgttc cagggttcga
     2041 cagaatcaat agaggataag gtgccaattc cagaaattaa catatctgca tgcgaagcgg
     2101 ttgctaaggt attagctaaa gaaggttcta gagccattag ttcacgtgca gtttcattga
     2161 tgacatataa aggaatactg agagtcaaat aagaagactc ataacgttca gcagcttttt
     2221 gaactaatcg tgtggagcca gctaaggaat tatattttcc gatttcgcca atgaattgag
     2281 taaaaattaa ttcttcacga gcacccattt taaactgatc aatcactttg taaacggtat
     2341 ctccccaaga tagtgcaacc acattggctt tcttaattaa ttcttgaatg taatcggcag
     2401 caaattttga aaataataaa tcgtgttgtg ataagtcttc actacttttt aatatataga
     2461 tattcgtttt aaagtaattt ttgaagattt cttctagctc ataattccta gcatacggag
     2521 aattaattga aatactaact aattgttttt cacgtgcatc ttcaatatat tttaaagctt
     2581 tatatctggt gatcccatat tttttagaaa tttcactaat attgatatcc tcgagataat
     2641 agtcttcaca aatttttaca aggagtgatt catagtcaga gaattccaga tgcatagtat
     2701 agc
//
LOCUS       DACECZ010000187         2650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.196, whole genome shotgun sequence.
ACCESSION   DACECZ010000187 DACECZ010000000
VERSION     DACECZ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.196"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            36..197
                     /locus_tag="IWO35_002656"
     CDS             36..197
                     /locus_tag="IWO35_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317675.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
     gene            210..713
                     /locus_tag="IWO35_002657"
     CDS             210..713
                     /locus_tag="IWO35_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0317676.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            773..1162
                     /locus_tag="IWO35_002658"
     CDS             773..1162
                     /locus_tag="IWO35_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0317677.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1149..>2650
                     /locus_tag="IWO35_002659"
     CDS             1149..>2650
                     /locus_tag="IWO35_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ0317678.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKG"
BASE COUNT          788 a          439 c          610 g          813 t
ORIGIN      
        1 aatggttttt aaccaatgcc caatccttag tcttactggc aattgtggtc attggtttgt
       61 ttttaggatt taagcgggag ttttccaaac tgattggctt tttggtgatt gctttgattg
      121 cggtcggatt ggtctttaat gcttctgggg tgaaggatgt cttactcagt ttgtttaacc
      181 gaattgtcgg tgcgtaaggg ggaagcacga tggaacagat gcgtgtttat attgcgaacc
      241 tcgggaaata taacgaaggt gaattggtgg gggcgtggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacgaatt gggttaaatg acgaatacga ggaatatgca attcatgatt
      361 atgaacttcc ctttgagatt gatgagtata cgccaattga ggaaattaat cggttgtgtg
      421 gcttagcgga ggaacttgaa ggaacgccag ttggtgaagt tgcttctgag attcaacatg
      481 ctttcttcaa ttcttttgag gagatggtag agcatgtgga tgatattatc tattatccag
      541 attgcgatga tatgagtgat gtagcatatt tcttgattgt agaagctggt gattttggag
      601 aagtaccaga acacttgaag acgtatattg attttgatgc gtgtggtaga gacttggaga
      661 ttggaggcaa ttatttggta acgagtcgag gtgtttttga gtatccatat taacaaaatt
      721 attgaagggt agttgatttc taaactatcc ttctttttaa aggaatgatt tgatgaagaa
      781 aataaaaagc tacaccagta tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt
      841 tcaactgcct tttcctgtga cttttaacca gatggcttgg tttgttttat ccttgctgtt
      901 tgtcatcgta tttgcccatg tgcctccact atctatgata gagggggcct ttttaaaata
      961 cttaggtata ccggtggcag ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa
     1021 accattggga tttttacgat cttttattag ctatcactta cgatttaagg tcactttcgc
     1081 aggaaagaaa gtcaaagaac agaagaaacg atgggacgaa cccattactc tggtaaggag
     1141 tgtgaactat gtacccgatt aagtatattg aaaataatct tgtctttaat caagagggag
     1201 aatgttttgc ctactatgag ttaatacctt acaattactc ctttttgtca cctgaacaga
     1261 aatatcaagt ccacgataac tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc
     1321 atgccttaca aattgccaca gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg
     1381 aaattaccgg gcgattaaca gaagtagcga aacaacgaat tgatttacag acagaggcat
     1441 tagtatccat gattggcgat agtcagattg attatcgctt ttttattggc ttcaaactga
     1501 ttgcgacaga tgaagaggtc aatctaaaga atctcaagaa atctttcttc tccggctttc
     1561 aagaatttgt ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg
     1621 aagaaattcg ccgatatacc aagcttgaaa aactaatgga aagtaaattg gctcgaagat
     1681 ttaaagtcag acgagtcaca ccgagtgatc tgatgtattt gattgaacat atctatggcg
     1741 aaaagggtac accatttgaa gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg
     1801 aaactttggt gaaacgttac gacttattac gtcccagtcg ttgtttgatt gaagaaaagc
     1861 cccggtactt atgcatggaa catgagaatc atgaatcata tgtagcgtac ttaacaatta
     1921 atacaattgt gggggaaatg gagtttccct cctcagaact tttctattac cagcaacaac
     1981 aatttacatt cccaattgac actagtatga atgtggagat tgtgacgaac aaaaaggcac
     2041 ttgctaccgt tcgcaataag aaaaaggaat taaaagactt ggacaaccat gcctatcaat
     2101 ctgacaatga aacaaattcc aatgtcttag atgcattgga ttccgtagat gaattggaaa
     2161 ctaccttgga tcagtccaaa gaatccatgt acaaactgag ctatgtggtt cgagtaagtg
     2221 ccgaatcagt agatgaacta aagcgccggt gtgatgaagt gctagatttt tatgatgata
     2281 ccaatgtaaa actagtccgg ccttttggcg atatgatggg gctacatgaa gaattcttac
     2341 cttctagtaa gcgatacatg aatgattata tccagtatgt tacgtctgat tttctcgccg
     2401 gacttggttt tggcgccact cagatgttag gggagttgga aggcatttac tttggttata
     2461 acgtagatac ggggcgcaat gtttacctga aaccggcatt ggcctcacaa ggggtcaagg
     2521 gttcagttac caatgcgcta gcagcggcat tcctcggttc actcggtggc ggaaagtcct
     2581 ttagtaacaa tctcttagtt tattacgccg tcctttttgg tggacaagct gtgattgtcg
     2641 atccaaaagg
//
LOCUS       DACECZ010000188         2643 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.197, whole genome shotgun sequence.
ACCESSION   DACECZ010000188 DACECZ010000000
VERSION     DACECZ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.197"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="IWO35_002660"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="IWO35_002660"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0317679.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          951 a          461 c          584 g          647 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaaaacttgg agataacatc
     2641 aaa
//
LOCUS       DACECZ010000189         2610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.198, whole genome shotgun sequence.
ACCESSION   DACECZ010000189 DACECZ010000000
VERSION     DACECZ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.198"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..527
                     /locus_tag="IWO35_002661"
     CDS             <1..527
                     /locus_tag="IWO35_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0317680.1"
                     /translation="DLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMP
                     SEQYGIAVHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINN
                     YVHLSPGVCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYG
                     NPAKIKKEGINFEF"
     gene            517..1659
                     /locus_tag="IWO35_002662"
     CDS             517..1659
                     /locus_tag="IWO35_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAQ0317681.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            1672..>2610
                     /locus_tag="IWO35_002663"
     CDS             1672..>2610
                     /locus_tag="IWO35_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0317682.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDK"
BASE COUNT          940 a          334 c          517 g          819 t
ORIGIN      
        1 aagatctgaa atatcttggg aaaataaacg aattgaatag ctgggcggat agcaaaaaaa
       61 aaatctataa atttattgta gctattggtg actgtgtagt aagaaaaaag attataaaaa
      121 aattgaatat gccaagtgag caatatggaa tagctgttca tccaagtgct atagtggcaa
      181 aagaaatttc aattggttat ggaactgtta tttttgcgaa tgctgtaatt aacccagatg
      241 ctgtaattgg agagcatgct ataataaaca caggtagcat tgttgagcat gattgtagaa
      301 ttaataacta tgttcattta tctccaggtg tatgcttagc gggtggagta catgtaggtg
      361 taggaactca aataggaatt ggttctcaat gtatccagat gaagaaaatt ggatcttggt
      421 gtatgatagg cgcagggagc actattgtta aggatattcc ctcacattcc ttagcttatg
      481 gcaatccagc taaaattaag aaagaaggaa ttaattttga attctagaat tttattatca
      541 agtcctcata tgggaggaac agaagaaaaa tatgttgcta aggcttttaa agataattgg
      601 atagcgccat taggtgaaaa tgtggataaa tttgaagaaa gtattacaaa atatactggt
      661 tctaagggcg gagcttctgc gttaagttca ggtacagctg ccatacatct tgccttaaga
      721 ctgcttaatg ttggaaaaaa cgatattgtc tttgctcctt catttacttt tatagctaca
      781 gtaaatccta ttttatatca aggggctgta ccagtgttca ttgactcaga acctaataca
      841 tggaatatga gccctgttgc actagagagc gcattagaag aagctgaaag aaacaaccaa
      901 ttacctaagg caatcatcgt agtgaattta tatggtcaaa tggctgatta tgaacgaatt
      961 ttagctattg caaaccaatt taatgttcca gtgatagaag atgcagcgga atcgttaggt
     1021 tctacttata agggaatttc gtcaggctta tttgggacaa ttgggatcta ttcatttaac
     1081 ggtaataaga tcatcacaac ctcaggtgga ggagctctag tgtctagtga taaaaaatat
     1141 gttgagcatt caagattttt agcaacacaa gcaagagatc aagcaccgta ttatcaacat
     1201 agcgaaatag gttataatta ccgtctaagc aatatttctg ccggtatcgg acgtggacaa
     1261 atggaagtat tgaatgatag agtagctaaa cgaagagaaa ttttcaatcg atataaggaa
     1321 gctttttctg gaattgatag aattgaaatg atgccagaag tggatggcat ggtgccaaat
     1381 cgttggttgt ctactttaac gatcaatgat tataattcta ttgatcaagt agttaacgta
     1441 ataaataaac taaatgaaaa aaacattgaa acaagagctt tgtggaaacc actacatttg
     1501 caaccagtat ttcaaggggt aaaaatgtat taccatgata ataatcttag tgtgtcagaa
     1561 aagttattta atagcggctt atgcttacct tctggaagta atatgacaga tagtgaacaa
     1621 gaatttgtta ttgatgagtt taaaaaaata ttcaagtagg tgtgatattt tatgaacata
     1681 ggaattattg gtttaggttc catggggaaa agaagactta gactcattca agaacacttt
     1741 aatgatattt tactatgtgg tgttgatact aatgaggcga gacgaaagga agttgaaaag
     1801 gaatttggaa taccaactta taatagtcta acagaggcaa aaaatttgtt tgaaataaat
     1861 tcagtttttg tttgtacatc ccctataacc catgaattta ttataaaaga ggctttaaat
     1921 ttagattgta atatctttac ggaaatcaat ttattaaatg aatattatga tgaaataatc
     1981 gaagtagcta aaaagaaaga tagattactt tatctatctt ctacattttt gaagagaaaa
     2041 gaaattcaat atattaagtc taaagtgacg cataataaaa aaatttctta tcaataccat
     2101 gtaggacagt atttacccga ctggcatcct tgggaagatt atcgcaattt ttttgtttca
     2161 aacaaaaaaa ctaatgggtg tagagaaata ttcgctattg aattaccttg gattatagat
     2221 acatttggga aagttaaatc gtttactgta atgacccaga aattatccaa tcttgatatt
     2281 gattattcgg acagtttcac tcttattatt gagcatgaaa ctggtatagt tggaacttta
     2341 agtgtagatg ttgtttcaag agtagctaaa agaaatttaa ctataattgg tgaaaagatt
     2401 ttaatagaat gggaaggtaa cccggattct ctattttcat ggaatgaaga aacttctagt
     2461 atgacagata ttcaattgta cgatgaggtt atgcatgata ataattattc acatactatt
     2521 attgaagatg catatttaga ggagattaaa gaatttttca cgcttttgga aaagaaaatg
     2581 gaaccccaat atacttttga aaaagataag
//
LOCUS       DACECZ010000190         2542 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.199, whole genome shotgun sequence.
ACCESSION   DACECZ010000190 DACECZ010000000
VERSION     DACECZ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.199"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            162..734
                     /locus_tag="IWO35_002664"
     CDS             162..734
                     /locus_tag="IWO35_002664"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0317683.1"
                     /translation="MCQKNCCHSIISKENLLNQDFSTETICEKWAADITYIPTKKNGW
                     CYLSSIMDLHTKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAR
                     EVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYI
                     EGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(1207..1779)
                     /locus_tag="IWO35_002665"
     CDS             complement(1207..1779)
                     /locus_tag="IWO35_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0317684.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1795..2400)
                     /locus_tag="IWO35_002666"
     CDS             complement(1795..2400)
                     /locus_tag="IWO35_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAQ0317685.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          798 a          510 c          402 g          832 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccat catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct
      241 gtgagaaatg ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact
      301 tatcttcaat catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa
      361 tgactgtgga ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag
      421 aaggaatgat tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat
      481 ggcttaaaac caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg
      541 ctggaatcga atctttccat gcctcattga aaaaagaaga agtctacacg actagctact
      601 cagattttga agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa
      661 atcgaatcca tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag
      721 aaaaaatggc ttaaccgtct ctactaaaat gtgtccaaga tattgactca aatccagtaa
      781 tatcacaata ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat
      841 ttggttctta aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc
      901 aatagagtca atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg
      961 acttacgctg aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat
     1021 gatggtatcc taatatagta agcgtggcta gcataccttt aattacttta ccttttacag
     1081 ttaccataca cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac
     1141 atgctaatag actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact
     1201 tcttttttaa ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc
     1261 ggttatagct tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct
     1321 cttcttcggt gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct
     1381 tgcaaacatt tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac
     1441 actcaacaac gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt
     1501 caaaaccttg atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct
     1561 cgtgttacga gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc
     1621 agccaacagc tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa
     1681 aatattcttc ttctctacgc agttctcaag caactgttga atttgttctt ttgtactttg
     1741 agaagcggtt gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa
     1801 atcatctatc gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat
     1861 atataactcg cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc
     1921 caaatcgttt atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt
     1981 ccaatctaca actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact
     2041 tcggccattt ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat
     2101 ctcttcaaaa gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat
     2161 aactggtgca aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa
     2221 atcgtaaata tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac
     2281 ttcgatttca tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt
     2341 ttcttcaacc ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat
     2401 gcgaaatctc cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg
     2461 cgattttgtt tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg
     2521 tctctatttt gcacagtcta gt
//
LOCUS       DACECZ010000191         2510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.200, whole genome shotgun sequence.
ACCESSION   DACECZ010000191 DACECZ010000000
VERSION     DACECZ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.200"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            96..1727
                     /locus_tag="IWO35_002667"
     CDS             96..1727
                     /locus_tag="IWO35_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ0317686.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLIIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1730..>2510
                     /locus_tag="IWO35_002668"
     CDS             1730..>2510
                     /locus_tag="IWO35_002668"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0317687.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEIL"
BASE COUNT          875 a          348 c          517 g          770 t
ORIGIN      
        1 gcaagtttat tcccttttta tattgacaac ggttacaacg tgttttaaaa tataaaatgt
       61 atcaaaacta acatatcagt ttgaggaggt taattatggt ttcgattaaa aattggcaaa
      121 attctatcga agaattcaat gaaatgcaga ttctactgcc gtgttttgat gtcaaagaag
      181 ttaagcaaga aggccagcaa catccagtat ggcttcattt tggtggagga aatctttatc
      241 gaggatttca tgcagaaatt gctcaatgct tagcagatca aaatgaatta gaaacaggag
      301 tagttgtgtg tgaaacattt gatgaagaag tagtggaaaa agcttatcat gactataaca
      361 atgacatttt ggaaattgtt atgagtggag atggtgaact caaaaaaaga cttttggcag
      421 taacggctga tagtttatat tgcaatccga agaatatagc ggactatcaa agagtggtta
      481 aatattttga agatcctaaa ttacagttta caacgtttac tattactgaa aaaggttaca
      541 gtttaaaaga ttctaaaggg caattctttt cggtagtcaa agctgatatc aaatctggac
      601 ctagcaatcc tcaacatacg atgagtattg taacatcctt gttatatagc agatttaatg
      661 ctggagagtt gccgattgcg atggtttcta ctgataattt ctcacagaat ggtcaacgtt
      721 ttcaagatag tatttttacg attgcagaag cttggcaaaa agaaggatat gtttctgaaa
      781 aatttattgc ttatttaaag gataaagaaa aagtgacttt tccttggagt atgattgatc
      841 gaattacgcc aaatccttca gagaatgtaa aggaagatct attgatgatg gggctcgagg
      901 atttagatat cattcacaca aaaaaacata ccaatattgc tgctttcact aatactgagg
      961 tagttcatta tttaattatt gaagatagtt ttccaaatgg ccggcctaaa ttggaaaagg
     1021 ctggtgtgat tatgacagat agagagacgg ttgataaagc agacatcatg aaggttacga
     1081 caagcttgaa tccacttcat actgctttgg cagtaacagg ttgtctgcta ggatatcaat
     1141 caatttcagc agagatgcag gatgatgatt tggttggttt aatcaaagga ataggttatt
     1201 tagaaggttt accagtagtt gaaaatccag gcattattga tccaaaacaa tttattgatg
     1261 aagtaattaa taaacgtttg cctaatccct atattccaga tacaccacaa cgaattgcaa
     1321 cagatacttc tcaaaaagta gcgattcgct atggtgaaac aattaaaaag tatgtcgaaa
     1381 aagacggaaa tgcagaaaat ctaacattta ttcctatcgc tattgctgct tggttacgtt
     1441 atttactggc agtcgatgat caaggggaaa attttgtgcc aagtcctgat ccgttattgg
     1501 tagagttgca ggataaatta tcagtattga aattaggtga acaagatcct tcaaagattc
     1561 attgtgcggt acaggaaatt ttagaaaata aagaaatctt tggtttagat ttatatcaag
     1621 ttggggtagg aaagaaaact gaaaaaatct tttgcgaaat gctagtaggt cctcaagcag
     1681 ttcgtaaaac aatcaataaa tatataacag aagttggagg aaagtaacta tggaaatgac
     1741 ttttagatgg tatggggcaa atgaagaaaa aatctcacta caagaaattc gtcagatccc
     1801 agcaatgaaa ggaattgttg gaacattaat ggatatacca gccggagagg tttggccgag
     1861 agaacgaatt aaatctttaa aagatgaagt cgaagccaat ggacttacct tgaaagttat
     1921 tgagagtgtt aatgttcatg atgatatcaa aattggttta ccaacacgag atcaatacat
     1981 tgagaattat aaacagacga ttcgcaattt ggcagaattt gggattgagg ttatctgtta
     2041 taattttatg ccagtatttg attgggtaaa atcggatttg gattatcgtt tagatgatgg
     2101 gtcttctacg ttagcctata tttcaaaaaa tatccctact gatccggagg aaattattag
     2161 aaaagtagaa aattctacta ataattttga attgccgggt tgggaacctc atcggttatc
     2221 acaaattaag caattattag aagcctataa agaagttgat gaagagaaat tacgagagaa
     2281 ctttgcatat tttctcaaag caattatccc gacatgtgaa gaagttggga ttaaaatggc
     2341 agttcaccca gatgatccac catattccat tttcggatta cctcgtattg ttaaaaatcg
     2401 tgacgattta gattggttgt gtaatgtagt ggattctcct tctaatggta ttacattatg
     2461 tactggaagt attgcagaac gaccagataa tgatgtctat gaaattctat
//
LOCUS       DACECZ010000192         2440 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.202, whole genome shotgun sequence.
ACCESSION   DACECZ010000192 DACECZ010000000
VERSION     DACECZ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.202"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            14..496
                     /locus_tag="IWO35_002669"
     CDS             14..496
                     /locus_tag="IWO35_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ0317688.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            533..1543
                     /locus_tag="IWO35_002670"
     CDS             533..1543
                     /locus_tag="IWO35_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ0317689.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1658..2371)
                     /locus_tag="IWO35_002671"
     CDS             complement(1658..2371)
                     /locus_tag="IWO35_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317690.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT          827 a          402 c          498 g          713 t
ORIGIN      
        1 aagagacata aaaatgattg acttggcaaa gaaattctta cacgaaaata tatattttga
       61 gttgtatcat catcgagcca tttatacaaa tgaagatgct ttggtaataa agaaagaaca
      121 aggatttaca ggtacagaga ccaaaagctt attcttaaaa gataaaaaag ataataacta
      181 cgtatatctg acttttacta cgaaacaaac ggatttcaaa gcattaggga agatcgtcgg
      241 taaaaggctt tctattgttt catcagaacg catggaagaa caaacgggac aaaagcaggg
      301 cgctgtttct ccgtttggtt atgaaaaaca gattccgatt attattgatg aagagctctt
      361 atcaaatgaa aaacttgttt ttgcaccagg tagaccagat caaacgatgg tcatccaaac
      421 ggttgactta aacaaaatca tccagctact tgagatgccg atatttttga tgccggcaaa
      481 cgaaaaaaac aattaaccaa gtaaaatagt gcatacttgg aggtgaagca acatgataag
      541 ttatccatta ttagaaagaa atgcaacgat tggggttact gcaccatcct caggcgtatc
      601 agaggaacac cattcgttac tcgaatcagc tttaaagaga atggaagaaa aatctttcca
      661 agtagttact ggaaagacgc tttggacgca agataaggaa aaatcaggtc cagcaagaaa
      721 acgagcagat gaattcaatc aaatgatgca agatgaaaat atccaaatga tctttccgcc
      781 ttggggagga gaattattga ttgagattct cgattttgtc gattttgatg ctctacaacc
      841 aaaatggata gtaggttatt cagatattag tacgttgcta cttgctgtca cattaaaaac
      901 agggatagcc actgcacatg gaaccaacct tattgattta agaggagagt acttggatga
      961 aacgacagca atgtgggaaa gtgtactgaa aacaagagaa ggagaatcga ttctacagca
     1021 accttctgaa aagtatcaaa aagagtggcc ccatgagcat tcatcatctt ctgttttcca
     1081 tctcactgaa ccaacaaatt ggcaatcagt cagcggagat gcagtaagga tgaagggacg
     1141 attattaggt ggctgtattg atacgatcag tcatttaatc ggtacccctt atggagatgt
     1201 ccaatctttt aaaagaaagt ataatgatgg aaagccgatc ttgtggtttc tagaaaattg
     1261 tgagctatcg tcagttgatt taagaagatc acttgttcag atgaaactgg caggatggtt
     1321 tgatggcagc tcggggatca tgttcggtag aagtgcagca gaggatttgt tagattataa
     1381 aatgaaggat gtatatgaag atctgtcaaa ggaattaaaa ctaccgatct tgtatgatat
     1441 tgattgtggt catgtaccgc cacaactgac attgatcaat ggtgcctatg cagaagtagc
     1501 ggtagaagat agaaaagcga ctgttttgca aacgtttatt taaaaaattt tttcctgcct
     1561 tggagtttat caaagaccaa caaatctaaa tagaaaagaa aatgaaagaa gctgacagta
     1621 ttttgccagc ttctttttgt atgatctatt agaaatttta attatccata actatcacat
     1681 tgatcagctg tttcacatca tttggattac ctgtaggaat agctactaca gatgcattat
     1741 caataatttc ttgagttgtt tctctaatga acgctggata agatgtttga tctgcaggca
     1801 tttgccaatg tgaaggaact ggataactag tgattgtccc gtcatggtta cttgaataca
     1861 ctaaaactcc ctggtaactg tatttcccag tgagcataac cacatctttc ggatagactg
     1921 cactccctgc atcataaggg gcgataggtg ttcctgcagg ataatgaata acatgtagtt
     1981 cgaaatcacc cgatgtatct ctaaaagtat tacctctaat tgctagccaa acacgtgcgt
     2041 actcaatttg tagatcagaa tatcctgcta acggatctat tgactgagaa gactgtgagg
     2101 aagttgtgtc tattgttgaa gaagaaatat ttgtctcaac tgtactacta gatgtagaag
     2161 aagtattggg ctctgagact ttactgcttg ttgaagatgt attcgtttca gtagaagctg
     2221 atttctgagt acttgatttt tcagctgaac tggtttgttt gattgaactt ttgtctgtgg
     2281 tcgcagatgg atcactattt tctttactag gagacgtaca tcctacaagg aataaagaga
     2341 ccaacaataa aataaaacct aactttttca tataaaatac ctcattattt ttttaatacc
     2401 agtatagcat aatcctgaat ttttgaatcc gtaaaacctt
//
LOCUS       DACECZ010000193         2429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.203, whole genome shotgun sequence.
ACCESSION   DACECZ010000193 DACECZ010000000
VERSION     DACECZ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.203"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(4..249)
                     /locus_tag="IWO35_002672"
     CDS             complement(4..249)
                     /locus_tag="IWO35_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317691.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(266..1117)
                     /locus_tag="IWO35_002673"
     CDS             complement(266..1117)
                     /locus_tag="IWO35_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0317692.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1283..2287
                     /locus_tag="IWO35_002674"
     CDS             1283..2287
                     /locus_tag="IWO35_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ0317693.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT          779 a          413 c          488 g          749 t
ORIGIN      
        1 tttctatttt ttcggtatca aggcaaaaac aggtagttca ggtgaataaa tgacttcatg
       61 ttcgtgccag ggtttgacca ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat
      121 ttgatcatgt gtccagtttc ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa
      181 gacaaaatcg gaatgaaaag tctgggtaac ttcttccgag agagaattca agcgttcgat
      241 tggatccata aaatcacctt tcgacttaaa attgggtagt atctgggtct tttgctttgc
      301 cgactacacc atctaactga tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa
      361 ataattcagt attttctttg attctgcttg gtgtcactga cttaggaaga ggaaggaatt
      421 cgtgttgcag cgaccaacgc aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga
      481 tttgtttcat ttcaggcaca tcgaagattt taccagtgcc taatggacta tatgcttcga
      541 ggatcatatc atgttttttt gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa
      601 ggaaaacttg attgaccatc ggcataattt ttgctgtttt tgctagtgta tctagatggt
      661 ggggtaagaa attgctcaca ccgattgctt tgattttacc tgcttcatac agttcttcaa
      721 atgctcgcca tgtatctgca tttgcttgtt cccaattatc acgaaaagca acagggtttg
      781 gccagtggat caaaaacaga tctaaataat ctgtttggag cttcttcaat gattcttcaa
      841 aagaagacat cacaagttca tagctgtggt ttgcattcca taattttgtt gtcaagaata
      901 tctcttctct aggaattcca ctttccttaa tcgcttggcc gacgctttct tcattcttgt
      961 atccttgggc agtatcgata tggcgataac ctgcagccag cgcttcttta acagaggaaa
     1021 cagcgacatc cccatcaggt gtttgccatg tgccgaatcc tactaccggg atttcgtaac
     1081 catttgatag acgataagtg cttgttaatg aattcatact ttcagctcct tttttattcg
     1141 ttcaactata agtgtagcgc atttttcgaa aaaaagaatc cattttgttt ctactcttga
     1201 aatttttgtt taaaagaaaa tgattttgat tctggtattt ttgatttttt ttccgtaaac
     1261 taagacagaa gggaatgaga aaatggatat ctatttaaaa aaagcaattc tgcatattat
     1321 tgacagagaa actggttctc cagtcttttc acaaaaagag ctggatctga caaaagaata
     1381 tattcgtgat tttcttcaaa agaagatcca aaaaatctct tctgctcaaa caaaaactgg
     1441 acaactagca gaagaaagca tgatcgttga atcacttaaa atcgctcaag aaaattttgt
     1501 ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc
     1561 acctagtgca gatgtatttg ttgtcttgta tgaattagat acagtgatgt acttatcttt
     1621 attgaaagtc aattatcgtg aggcttacac tcactttgtt gaagcagacg aagcagggat
     1681 cgacaaccgc ttgattttga atcgagcaat tctaggggcg aaaacacaaa aagcagatga
     1741 agcattcgct gtgaatttga ctgattggac ctacgaatta attgaaaaaa aatatgagtt
     1801 ttctggcaaa aaagagcttt atttttctgg aagagtgatc gaaagtgttc ctgcaccttc
     1861 tttagaagaa aatgtaaaag tgatccaaaa agtagccaag catcttggaa aaaaatttga
     1921 gacagaagaa tttgatatca tggctgattt gaaagaagct gtctatgata cgatagaaga
     1981 aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc
     2041 aaaaatggct tttaaagaag aagtacaaga gcaaggattt gtcccagaag cacctcctgt
     2101 aaaagaagta caagaaattt ctgagaaaaa atttggaaaa caaaaattca agctatcaaa
     2161 cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac ccagatttga ttgaatttgt
     2221 gaataatccg gatggtacga tatccgtgat gatcaaaaat gtggatgaag tattgaatcg
     2281 tttataaaaa tgactagaat attaggtgtt caagtaccag tgatcagtta aatgaattgt
     2341 gtcaagagaa ggatcggaaa agacgaatat aacaaaaaaa gagcgtgaga caaaagtaaa
     2401 gaacactttt gtctcacgct ctaaacacg
//
LOCUS       DACECZ010000194         2400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.204, whole genome shotgun sequence.
ACCESSION   DACECZ010000194 DACECZ010000000
VERSION     DACECZ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.204"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            207..2069
                     /locus_tag="IWO35_002675"
     CDS             207..2069
                     /locus_tag="IWO35_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0317694.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="IWO35_002676"
     CDS             2069..>2400
                     /locus_tag="IWO35_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0317695.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DACECZ010000195         2393 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.205, whole genome shotgun sequence.
ACCESSION   DACECZ010000195 DACECZ010000000
VERSION     DACECZ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.205"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            70..282
                     /locus_tag="IWO35_002677"
     CDS             70..282
                     /locus_tag="IWO35_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317696.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            444..962
                     /locus_tag="IWO35_002678"
     CDS             444..962
                     /locus_tag="IWO35_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAQ0317697.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            969..1481
                     /locus_tag="IWO35_002679"
     CDS             969..1481
                     /locus_tag="IWO35_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317698.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1752..2375
                     /locus_tag="IWO35_002680"
     CDS             1752..2375
                     /locus_tag="IWO35_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0317699.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          914 a          300 c          405 g          774 t
ORIGIN      
        1 aatctaatta gcttttttag tcaatctaaa gttctttatt ctatgatata atgttactgt
       61 agaaagtttg tggcggtttg gcaaatccaa ttgaaggagc gtggtgccta tgggcttatt
      121 agcttatcgg acgtactctg tagaaaggaa atgcctgatt tgttctcatt ggatcaaatc
      181 attgctatta tcggagcagc agcagtagtt attactgccg tagcgaaagc tagcgattcg
      241 attttagctt ggttaaagtt caaaaaagaa caaaaaaaat agagccgtct caacttttgg
      301 cgaggtagac gagctctaaa gctttattta catgccatcc gctttgaacg gactacactg
      361 ggcatgtgac agcatgccct ttcatttata ttatatctca ttcttttata taatgaaagt
      421 aaataaatca tggaggataa aatatggata aatttaatag attaacatta ataaatcagt
      481 ttgaaatttt aaaagcttta aatcctaacg aagaaaaata ctatgctgaa aaaatagaaa
      541 tactaacaga aggttacgaa tatcactata gtgaaatatt tgaaaatatt tctgatccat
      601 tgccagaaga agattcgcgg tttgttctag acatattaaa tatgtataga gatattactt
      661 ttagtaaaaa taaattaaaa gatataaatt caattgataa ttattatata caatttaaag
      721 gttttgactt taatagctct accgaattta agttagcact ttatgcacaa ttttttattg
      781 aaaaattaaa taggtttcaa gaaatagttg aagatagtga ttttaacact tttaatagcc
      841 ataaaccgat gagaggtact tatataaaat ttctcgaaaa ttataatgat ttaaaatcta
      901 ccaataatta tcaatttgga aacttgtctt atgagatgat aagtaaagtt ctaagtttgt
      961 gatgataaat gaaattaaat agtataaatg atttatttgc tattataggt gttattgcta
     1021 acatactttt ttcgtgtttg aattttagat ttactcaaaa gaacaaaaag aacaaactca
     1081 gtatagaaca aatgaaaaaa gacattgaaa ctgatttaaa aaatgaccat aaagaaagaa
     1141 gaaaaattga agaatatact tctttaatgg aaaagtgtaa agcaactgat gcaattttta
     1201 gctttttaag aaaaagtgat gagataatca cacgtgaaaa tatagattta gaaaatgaga
     1261 ttagtaaact tgaatctatc ttttccgata taaattctct taagtattta atcgtcgaag
     1321 aaaattcgga tagcttttta gaatttgaaa aatatctaac agatcttata gataatagaa
     1381 ataatgaatt aatgagacaa aacattgcaa gattccttac taaattagtt tctgaatgga
     1441 aaagtattat ttctcaaaat atggataaat taagatatta aggatgttag ttttaaaata
     1501 aactaatgtc cttatttttg tgttcacatt tctgttacct aagaaatact taaaggaggt
     1561 gatgtagcca gcaacgacta caacttgaac ccgctatcta cagtttattt gttgtttctt
     1621 ttgggtatac tctaatggta caaatccata tttgcttaaa gcgttgattt ttcagcggtt
     1681 tttctttatt gcaaagtgta ccctaagacc tgtatactaa tagtaaatag attggatagg
     1741 aatgatggca aatgtattta gcttatttaa gagtttcttc cgacgatcaa agtttagctc
     1801 ggcaacataa gctaattgaa aattggtcag tcaaaaataa tattgctgaa gacgatttaa
     1861 aaattttcga ggaaaaagtc tctggaaaaa atattgaaaa cagaccagag ctgcaagagc
     1921 ttatttcttt catacgtgaa cgagatacga ttattattcc tagtcttgat cgattagcaa
     1981 gaaatagtaa agatacaaaa gacttacttc aattgtttcg tagtaaaggt gcttctattg
     2041 aaatattaga cctaccgtcg tttaatgggg taactgaccc tgcattaaga gacttgctaa
     2101 caaatttagt catagaagtg tttagctatg ttgctgaaaa cgaacggaaa aaaatcaaag
     2161 agcgtcaaaa acaaggcatt gaaattgcaa agaaaaatgg tcgttatact ggtaaaaaga
     2221 ttcaatacca tgccaatgca acaggaaaaa acaaattgat ttacgatgaa attgttgatg
     2281 gtctgaagaa aaaagaaact atttctaaaa tagcaaagaa cgcaggagtt acaagaaaaa
     2341 ccgtttatcg agtaaaaaga gatttaggaa tgtaatatgt tccagttaga aag
//
LOCUS       DACECZ010000196         2359 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.206, whole genome shotgun sequence.
ACCESSION   DACECZ010000196 DACECZ010000000
VERSION     DACECZ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.206"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..321
                     /locus_tag="IWO35_002681"
     CDS             <1..321
                     /locus_tag="IWO35_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0317700.1"
                     /translation="YKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEELVMLFLDPDFI
                     GKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLDGFGKPYPITH
                     LVKK"
     gene            735..1553
                     /locus_tag="IWO35_002682"
     CDS             735..1553
                     /locus_tag="IWO35_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0317701.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1626..1985)
                     /locus_tag="IWO35_002683"
     CDS             complement(1626..1985)
                     /locus_tag="IWO35_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0317702.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          791 a          338 c          432 g          798 t
ORIGIN      
        1 tataaagcaa tcattcctga gaacttagat gaggttaaac tttatttatg ggaagataac
       61 ggagaaattg tcggttttag tggtgtaagt gcagaagaat tagttatgct atttcttgat
      121 ccagatttta ttggtaagcg atatggtagt caaatattaa ttgagctgat tgaaactgaa
      181 gatataaaaa gaattgatgt taatagtcaa aataaatatg caaaacattt ttatttaagc
      241 catggatttg aaattgaatc tgaagaagac ttagatggtt ttggtaaacc ataccctata
      301 acgcatttag taaaaaaata attcatgaat ggtttattac ctgattgaac atgaataaaa
      361 gtaatatttt tattttgatg gcctatttct aggttatgaa ttatccagat caaatttgtg
      421 tattacattc agtaataagg tgcttatctt aggatcaact tatgatacta gctgaccaag
      481 gatgtaccag ttatagaaaa tcagttaaag taaaaatgat aaaaatctta tgtcaacact
      541 gatttaggct gacctgtggg ttaggagaaa gacaggtcgg tagcttttaa tacaaaaaat
      601 ggcttgtatg tatctagata gttaactgaa attagaaatg ttcttatttt taacggagta
      661 agaaaaatag gctgctttag gtctgaatac tttttcaata taatgaagat atcaaaggcg
      721 acaggagtgt ttttatgaat aagaatcctc ataatctaaa gttggtagca gtagatatgg
      781 atggaacatt tgctcgtact gattacacat acgatatccc aaggttcaag gctgttttat
      841 cgcgaatgaa agctgttgat tgtcaatttg ttgttgcaag tggaaatcaa tactaccaat
      901 tgagatcgtt atttccaaat tattatgatg aattaagttt tgttgctgaa aatggtgctt
      961 ttgttaaaga taaaacagag gtactatttt cagccgatat gcctagaaaa gcgatcgagg
     1021 tggtccttga tttatgtaaa gactatcctg aagtaaaaaa tgtgatgtgt ggtctgaata
     1081 gtgcctactg tgaaagagga aaggtgagtc aagcattttt tgatctaacc aatatctatt
     1141 accatagatt agaatgggta gctgattttg cagaagttca agataaaatt ttaaaatttg
     1201 ctccgactat gcctgttgaa aaaaccgaat tttacgttga attgttccgt gaaaaattag
     1261 ccgggctatt agtacctacc agtagtggtc atggttctgt agacttgatt ctttctgagt
     1321 gccataaaac tttcggcttg aaaatgttag ttgagcgctt ggggattaat cctgaccagt
     1381 gcgttgcttt tggagatggg ggcaatgaca ttgagatgtt ggagtattgt ggtctgagtt
     1441 atgaaatgga taatgcaacg gaagctgtta agcaagttgc taagcatcaa tgtccttcaa
     1501 atgacgaaga cggtgtttta gtgacgctcg atcgattgtt ccctaacacg taaacactag
     1561 gtaattcatc aaaaaacctc gatataagaa atattcgagg ttttaaaact atatttagaa
     1621 cagatttacg aagcgtctga tgagtcaaaa tagacttgga cattgctatc taaaatacca
     1681 ctttcattct ttaaacgaat tgtttgattt gatggagtta cctctaatac taaatgccca
     1741 ttaataaagg tagtatcgta ttcagatgtt ttaactgttg cttcttcgca aggtattgtt
     1801 aaatcatatg ctggaatata atgttgtgtg gaaactaata aaaaatgatc accaatcata
     1861 cagcatccct ctgctgaaga aaattgatta gagccatcat ttaaagttaa caagaatcca
     1921 tcctttaaac gaggtacttg cttaaatact tctggttgta cttctaaaaa taaattatcg
     1981 ctcattttca cgaccaccat cgacttccac agttgaaatt gacattttat ttaacacacc
     2041 tattccccct taactataac tcaattctat ataaaaaata ttctattctc aaataattga
     2101 acttttcaaa tcgccaattt acttcggctt tatcagagct cattcatatc aagagcgatt
     2161 aacgctgtaa aaattaaacc gaagatctga taaattttct ctattttcta caatatttaa
     2221 aacgcttcct tagtctatta tatatctatt tctcttagga gtatgtttag atgtttgaat
     2281 aatagttgcc caactttaaa ggttattctt ataatatttt acttcatttt ccaatagaaa
     2341 attcaaagga ttactggtt
//
LOCUS       DACECZ010000197         2304 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.207, whole genome shotgun sequence.
ACCESSION   DACECZ010000197 DACECZ010000000
VERSION     DACECZ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.207"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1005
                     /locus_tag="IWO35_002684"
     CDS             <1..1005
                     /locus_tag="IWO35_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0317703.1"
                     /translation="EVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPD
                     ASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRS
                     DADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVV
                     TYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHH
                     GTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKT
                     DNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE
                     "
     gene            1139..>2304
                     /locus_tag="IWO35_002685"
     CDS             1139..>2304
                     /locus_tag="IWO35_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0317704.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVG"
BASE COUNT          917 a          293 c          448 g          646 t
ORIGIN      
        1 gaagtaacgt atccagacgg tacgaaagat acggtgaaag ttccagtaga agtgacagac
       61 aatcgttcta atgcagataa atacaatcct gaaggacaaa aagtgactac agacttaaat
      121 aaagagccag atgcatctga gggaattaag aacaaagaag atttaccaaa aggaactact
      181 tatgcttgga aagagaaagt agatgttagc acagctggaa ataaaaaagg tactattgta
      241 gtgacatatc cagatggatc atctgatgaa gttgaagtag acgttacagt gacagatact
      301 cgttccgatg cagataaata taatcctgaa ggacaaaaag tgactacaga cttaaataaa
      361 gagccagatg catctgaggg aattaagaac aaagaagatc taccaaaagg aactacttat
      421 acttggaaag aaaaagtaga tgttagtaca gctggaaaca aaaaaggtac tgtagtagta
      481 acatatccag atggatctaa agaagaagta gaagttacta tttctgtagt agataaaaaa
      541 gcaccgaata aacctcaagt tgatcctatt acagagggtg accaaattgt tactggtaaa
      601 actgaaccaa atgcagaggt gacagttaca ttaccagacg gaagtcaaca ccatgggaca
      661 gctgataaaa atggtaattt tacagttaaa gttcctaaat tagaggcagg gacaaaagtg
      721 atagtaactg caactgatga atctggtaat actagtgaac ctactaatgt agttgtttct
      781 tccaacaaaa aagacagtgg aaaaaacggt agtaaaggta gtaagactga caatcaaggt
      841 agcaacagca atcaagacaa aaatagaggc aaaagccagt cctcaaaagt ttttcctaag
      901 acgggagaga gtgacagtaa tatctttact attagcggtg gtttaatact gttaggaacg
      961 ttaggattat tagggtataa aaatcgaaaa aaagagaatg aatagaaaat caatttctta
     1021 agtcttctct gagaagagga agaatctttt tcttcctctt tttgcttttt tatgatggga
     1081 aataagtgtt agttaaagat gaatatactt tttatagaaa ggaaaagaat gtaattaagt
     1141 ggaaaaaata gtaattattg gttcaaacca ttcagggcta gccgcagctc gatttctaaa
     1201 cttaagcgat tcaaattatg aagttattct aatcgataaa aaatcagaaa ttagttactt
     1261 ggaatgtggg acatcgttat ttataaaagg agaagtaagt aatttagaag atctttttta
     1321 tcaaaataga atgacactaa attctgagat aaaaaagtta tttctagaga cagaagttga
     1381 caaaattgat attcaaaata aaaagattag ttgtgtaaaa aaagatggta caagattctc
     1441 tcaagaatat gacaaagtta tattagctac aggttcaaag caaattatgc tagatatacc
     1501 ggggaataat ttagctaata ttttttcgat aaagaatttt ccaaatgcaa aagagatttt
     1561 taaagctttg gatgatagta ctattaaaaa agtaggaatt attggagcag gatatattgg
     1621 agttgaaatt gctgaagcca taagaaaaag agggaaagaa gtatatcttt tcgatgttgc
     1681 agatagagtg ttgtcgactt attacgaccg tagtttttca gataaagttg aagaaatact
     1741 atcaaaaaat ggaatacatc tttgtctatc agaaagtcct aaaaaatatt taggaagaaa
     1801 aaagattgaa aaaatagtca ctacaagtgg tacccaatac tctatggata tggtcgtaca
     1861 agctatagga tttctatcta atagtccaat tggggaaaag gaactcttga gagatgttag
     1921 tggagcatat ctaactaatg aataccagca aacaaatata aaagatatat atgctattgg
     1981 ggattgtgct acaacttttt ttacgtccat taatagaagt agtgttgatt tttctatttc
     2041 taatgctcta agaacagctt atattgcaac tcaacatatt aacaatcaag attttacacc
     2101 ttctggttcc caatttacaa atggcttttc tatatttaat tacaattttt acgcagcagg
     2161 tttgaattta aaaacagcac gattattaag tgagtctata gattatatag aaatagaaga
     2221 tttcattaga cctgcttttt tgagaaaaaa tagtcgtgta aaattaagaa ttagctttga
     2281 aaaaaataca aagagaatag tagg
//
LOCUS       DACECZ010000198         2247 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.208, whole genome shotgun sequence.
ACCESSION   DACECZ010000198 DACECZ010000000
VERSION     DACECZ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.208"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(954..1244)
                     /locus_tag="IWO35_002686"
     CDS             complement(954..1244)
                     /locus_tag="IWO35_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317705.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1452..2144)
                     /locus_tag="IWO35_002687"
     CDS             complement(1452..2144)
                     /locus_tag="IWO35_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ0317706.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          637 a          424 c          332 g          854 t
ORIGIN      
        1 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
       61 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      121 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      181 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      241 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      301 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      361 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      421 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      481 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      541 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      601 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      661 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      721 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      781 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      841 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      901 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
      961 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1021 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1081 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1141 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1201 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1261 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1321 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1381 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1441 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1501 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1561 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgagtttc tacatttgtt
     1621 ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct
     1681 gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt
     1741 tcatcatttt tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt
     1801 agttgtgcat ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga
     1861 tttttttggt tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa
     1921 tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa gatctgtaag cgcttgctct
     1981 ttatctgtca atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc
     2041 tctccacccc acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat
     2101 agaaatgcaa cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa
     2161 tccaatactt ttacatgcta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa
     2221 ttacatgtgt aattttaata taaatac
//
LOCUS       DACECZ010000199         2233 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.209, whole genome shotgun sequence.
ACCESSION   DACECZ010000199 DACECZ010000000
VERSION     DACECZ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.209"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            405..860
                     /locus_tag="IWO35_002688"
     CDS             405..860
                     /locus_tag="IWO35_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317707.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(1022..2194)
                     /locus_tag="IWO35_002689"
     CDS             complement(1022..2194)
                     /locus_tag="IWO35_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0317708.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
BASE COUNT          720 a          443 c          397 g          673 t
ORIGIN      
        1 aacgtacata ttttattatt gcatacttaa taacagaact atagcaaaaa atcccgtgta
       61 tcaaaacggg attagattaa gtttcctcaa tcactattca taacacaatt attataacaa
      121 gtaagtaaac aattgaaaag gacatttatt gaaaacggtt gttttttact taaggaggaa
      181 atttagtgaa aaaattagtt gcattgtttt tggtcgcagg aagtttgttg agtgggtgtg
      241 gtgctactac gtctactact caaagcacaa gttcagctca ggcttccagc agcagtacca
      301 cgatctccga aagtacggca gattcatcga aagaacaaga acaaattgaa aagaaaaaag
      361 aagaagagaa acaaaaagcg gaaaaagctg cactagaaaa aaaagtgaca gaagcggaat
      421 ctgctttgtc acaagctgaa gcaaatccta caaaagaaaa ctatgcaact gcttcagcag
      481 ctatccaagc gctacctgaa aacaaacaag agttgacaag ccgtcttgct gcaattgatt
      541 ccacaatcaa agcaaaagaa gcagaagaag aacggaaaaa gcaagaagaa attgcacgac
      601 aacaagcaga acaagcgcaa caagaagcaa tcgcacagca acaagcagaa caggcgcgac
      661 agcaagcagc agctgaacag gcctctcagc aggcacaagc cgcagctcct gagcaaaata
      721 atgaacaaac tgtctatgtc acaccgaccg gttcaaaata ccatacacat aaatgtggaa
      781 atggtacgta tacccctgcc actttaagcg aagctcaagc tcgtggttta accccttgct
      841 ccaaatgtta tggaaactaa ttcaaaagtt atactataat aataaaattc ttctgaaaca
      901 taaaaaaata gaagctggcg ttccagatag taaaccaaaa atagcagttt acacgtccat
      961 ttcgtcagct tctctctttt ttcatttagt ctgttccctc tgcttttttg gacatcttca
     1021 ttcattcttt ctcaatcaaa tagtacacct ctttttcttt tcgcagttta tatcttcctt
     1081 ttttcaattt cattgaaaac tgtttcgaat gtttatgttt tacatggatt ttaagcagga
     1141 gctcctcatg ttgcaagcga tagttcctgc cttttaaatc agaaaatctc actagtccgt
     1201 attcatcaga agctttcaca aatggaactt tttctctagt aatatagtgt ttgccgttga
     1261 gcgtttttac agaaaagacc attccttcga ttggtcgtcc gtttgtgttc tttacataaa
     1321 aagataaagg atagtttcgc ttagcgatgg ccaaagaatc taaataagcg atgagtagcc
     1381 aaactaagcc gattgatcca agtagtatac cgataaacca aataaaaaat ttccattttt
     1441 tccattgatc aattccttgg atcgcttttt ttgcctcttt tgcttgataa ggaatacgat
     1501 gccccgtcac aagcagtcga tgtgtattga ccatataagg agtacaggta agcaaagtca
     1561 cgagatctct gcctttttgg atacccaaag aatctacttc atcaggtaag accacttgga
     1621 ttttttctac ttgatacgcc aatgtctttc cattgatctg gatgaaaaac tggtctcctt
     1681 ttttcaactt aggcaaatct gtaaataatt ttgcttctgg gagtccacga tgcccagaaa
     1741 gaacggcatg ggtgtttttc ccgccagtcg gataagaact ttcttccaat aatgacgttc
     1801 ctttttgcaa aaatatttcc gttgtctgat cgaatatagg taagctgaca ttgatttttg
     1861 ggatttcgat gactccaagc atatgttcca taaaaaaggc attactaggt aaatctttta
     1921 atgcttttgc atcaactgct tggttgaaac tgcctatccc tggtgatacg ttttgctctg
     1981 ccaactgttg gtttttcttt gtcatttttt cctgctgttt ttttatttcc tctgaattct
     2041 tattcgatgc ttgcttttga taatggatga tcatttgctg ggcaagaaaa tcattcagtg
     2101 aatcttctac aaaaggataa gaaaagatcc cgatcccaac gactaaaatg attgccatga
     2161 agatgtccag tattcttatt ctgcttttat tcatcatgtc cacctatttt ctaatatgta
     2221 tatgaagagg ttg
//
LOCUS       DACECZ010000200         2224 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.210, whole genome shotgun sequence.
ACCESSION   DACECZ010000200 DACECZ010000000
VERSION     DACECZ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.210"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(174..1043)
                     /locus_tag="IWO35_002690"
     CDS             complement(174..1043)
                     /locus_tag="IWO35_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0317709.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(1095..2087)
                     /locus_tag="IWO35_002691"
     CDS             complement(1095..2087)
                     /locus_tag="IWO35_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0317710.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETIEKRTVLPIEFGEGDTTKPLVRNDFA"
BASE COUNT          650 a          493 c          350 g          731 t
ORIGIN      
        1 tggtaaaaag aactttcttg tactcaatat catttcgtaa ttatattaat ttgaaacaga
       61 ataaaagtct ctactcagat tctcaagaaa gaagaatgaa ttgagactat ttaagtttca
      121 gaataccctt ttaaacttcc ggtatgttat gaatagttct aataaatact attctaatga
      181 ttgagtttct ttattgctaa agctaggcag ccaatcaagc ccgcattgtc agcaagcctt
      241 ggagttagta gatattcttc gagagtaggc gtatccacgt aggaacccat tttttcttta
      301 aacgattggc gaatcaactc gagtagttgc ggctgtttca ttacaccccc gcctaaaatt
      361 attacttccg gtgcatatag taacgttgtg ttatacacac actgagcaag ataatctgcc
      421 tcaatcgtcc aataaggatg atcaaatgag atagtctggg cttttcttcc aaagcgtttt
      481 tcaatcgcag gtcctgatgc taacccttcc aaacaatcat gatgaaaagg acagattcct
      541 tcaaattgat cttcattatg acgttgtaag agcatatgtc ccatttcagg atgactaaaa
      601 cctgcgacta ttttcccatc cactacagca ccaccaccaa cacctgtacc aacggtgtaa
      661 tacagaacac tatttttacc ttttccatac cctgccatat attctccata acaagcagca
      721 tttacatcgg tagtccaata gatgggaacc gacaaggcgt tttccaaagc tcctacaaaa
      781 tgataatttt gccaagctaa cttcggtgtg ttggtgatat atccgtaagt tcttgattcg
      841 atttgaatat caataggccc aaatgctcct acaccaattg atgcgatatt tcctttgtac
      901 ttatcaaaga aatcaaacac ttgctccatc gtttcttctg gtgttgtggt aggaaaactt
      961 ttccgttcta tcacttgtaa ttgttcatta ccaatcccac agacaaactt tgttccaccg
     1021 gcttctattc caccatataa cattttatct acacctttca ttcactcatt ttcaaaaaaa
     1081 gaaacgtatt tgcactaagc aaaatcgttt ctcactaaag gtttcgttgt gtcaccttcg
     1141 ccaaattcaa tcggcaatac ggtacgtttt tctattgtct ctccattaat gacagcaatt
     1201 aattttgcca ctgccacttc cgccatttgc tttgtaggtt gggcaatcgt tgagactaga
     1261 taaggatctc cttctgtcat cttttgacca tcaaatccga taacttggac atcctctggt
     1321 acgtttttcc ctattttttc aagcatatca atgacagcta atgccattgg atcgttgatc
     1381 gtaaaaatcc catcgatatc gggattctct aacaaaaaat ttgttacacg ttctgagaga
     1441 tggtctaaag gttctaacat atctaacgct ttataggaaa tccccagttg acggcactcg
     1501 atttcaaaat attttcgacg actcttcgtt tcattcggtg tttcttggta cccaccgata
     1561 tatgctacat tctgacatcc tcggcgataa agttcatttg cagccaaacg tccaccggcc
     1621 gcattgtcag cggtcacata aatcacctct tcagtgaaat aacggtcaat actaacaaaa
     1681 ggcagattac tcgaaatgta ttcatcgata tctgaataag taattccaat gatgccgtct
     1741 actttatttt ggctgaccat ctggatatat tcttgttctt tttcactatt accactggag
     1801 ttacacaaat aaagcttgta gttttgtctt tctaatgctg tttctacatg gtaggcaaat
     1861 tcgccaaaaa atggatgcca taccgtgggt agaatcaaag caaccgtgtt cgtatgcttc
     1921 atctttaacc cccgggcata aaaatctggt tgatagttta actctgtcat tgctgcttca
     1981 actttttctc tagtagaagc tttaacagaa ccgttattca atacccggga aacagttcct
     2041 actccaacac ctgctaattt tgcgacatct ttcattgttg ctttcatagt tgatccctca
     2101 catttctgca caagtttagt ataatgaatt ttgtcataaa ttactaattt tttcaaaata
     2161 ctaatagcaa atccatggga aatctagcta tctgttcttt tctaaaaaca ttatccttca
     2221 aaac
//
LOCUS       DACECZ010000201         2192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.211, whole genome shotgun sequence.
ACCESSION   DACECZ010000201 DACECZ010000000
VERSION     DACECZ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.211"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..354
                     /locus_tag="IWO35_002692"
     CDS             <1..354
                     /locus_tag="IWO35_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0317711.1"
                     /translation="RLVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALV
                     SDAPIILADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELS
                     GKKLKKVNKMNLEVE"
     gene            354..1730
                     /locus_tag="IWO35_002693"
     CDS             354..1730
                     /locus_tag="IWO35_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0317712.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            1860..>2192
                     /locus_tag="IWO35_002694"
     CDS             1860..>2192
                     /locus_tag="IWO35_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0317713.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT          854 a          271 c          402 g          665 t
ORIGIN      
        1 agattagtaa ataaatcagc agatgaaagt atcttgtttg aactaggttt agataaaaaa
       61 caaataaaaa gaaatgttat gaaattatct ggtggacagc aacaaagagt agctattgct
      121 agagcattgg tatcagatgc tccaataata ctagctgatg agcctactgg taatctagac
      181 agtgttactg ctggagaaat aattaatata ttaaagacat tagctaaaga cagaaataaa
      241 tgtgtaatag ttgtaacaca tagtaaggaa gtagcagatt ctgcggatat cattttagaa
      301 ctaagtggta aaaagttgaa aaaagtaaat aaaatgaatt tggaggttga ataatgataa
      361 aaaatgcatt tgcttatgta actagaaaaa gtttaaaatc attaattatc atattagtta
      421 ttttatctat gtcaacttta agtctcatta gtttatctat taaagatgct acggacagag
      481 cttcaaaaga aacatttgct aatataacaa atagtttttc tatggagata aatagacaag
      541 taaatccagg aacacctaga ggtgggggaa atgtaaaagg tgaagatatt aaaaagatat
      601 ctcaaacaga tagtatagac tcctatgtaa aaagaataaa cagtgttgca gatttagttg
      661 attatgatat tattgaaact caagagactc ttgcgaatca atcacctgaa agagcaaaga
      721 attttaaaag aacagttatg ttaactgggg ttaacgactc aacaaaagaa acaaaatttg
      781 tatcagaagc atataaatta gtagaaggaa agcatttaga aaatgaagat aaaaataaaa
      841 tcttaatgca taaagattta gctgaaaaaa ataaccttaa agttggagat aagataaaaa
      901 taaaatctaa tttatttgat gctgataatg agaaaggtgc agatgaaaca gtagaagtag
      961 aaattaaagg tctattcgat gggcataata gcggtgtagt aagtgcagca caagaactat
     1021 acgaaaatac attaatcact gatattcata cagctgctaa agtttatggt aataccgaag
     1081 atacagctgt ataccaagat gcaacattct ttgtaaaagg tgataagaat cttgatagtg
     1141 taataaaaga tcttgggaaa ttagatataa attggagaga atataatttg attaaaagtt
     1201 catcaaatta ccctgcatta caacaatcta tatcaggtat ttactcaatt tcaaataaat
     1261 tattttttgg ctcattaata tttgcaggag ttgtagtttc attattatta ttcttatgga
     1321 tgaatgctag aaagaaagaa atagcagtat tactatcatt aggtatatca aaattagaaa
     1381 tttttggtca attcctaatt gagatggtat ttatatcaat ccccgcattc gttggttcct
     1441 atttcttagc tcaatataca gctgacaagc ttgggaataa tatattgaat aaggttactg
     1501 gtgatatagc taaacaaata gctaaacaat ctgcatctag ccaattaggt ggtggggcag
     1561 aagctgaagg atttaacaag acattatcaa gtttagatat caatgtatta cctaaattca
     1621 tgatttatgt agttatattt atgagtttag tacttcttgt atcattgatt atttcttcat
     1681 tcaatatatt aagaaggaat ccaaaagaat tattaattga caataattaa aattttcggt
     1741 gctttttagc accaaaaatg agagtgtaat ataaaccgtg taagtaacca tcaatcctag
     1801 gcgggtggtt gcttcacggt ttttattttt ctaaaataag atcaagaggt gattacacga
     1861 tggctagaaa gaaaagaaat cctgatgctg agaagttagc tgaatccatt ttgaatactt
     1921 atcaacccga atctgtagag gatatgcaag atgttttaaa agatgtgttt ggtcccttat
     1981 ttgaaaaaat gcttcaagga gaattgaata accatttagg gtatgacgcc cattctaaag
     2041 agccaaagaa acatgacaat cgacgaaacg gttatggcaa taagacgctt aaaacgagct
     2101 ttggtaaagt aaacattgat gttcccagag accgggaggc ttcttttgaa cctgagttga
     2161 ttcctaatag aaaacgagat gtctccgaca tc
//
LOCUS       DACECZ010000202         2180 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.212, whole genome shotgun sequence.
ACCESSION   DACECZ010000202 DACECZ010000000
VERSION     DACECZ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.212"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            700..>2180
                     /gene="ltrA"
                     /locus_tag="IWO35_002695"
     CDS             700..>2180
                     /gene="ltrA"
                     /locus_tag="IWO35_002695"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0317714.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKP"
BASE COUNT          858 a          336 c          455 g          531 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc
//
LOCUS       DACECZ010000203         2164 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.213, whole genome shotgun sequence.
ACCESSION   DACECZ010000203 DACECZ010000000
VERSION     DACECZ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.213"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            18..>2164
                     /gene="acm"
                     /locus_tag="IWO35_002696"
     CDS             18..>2164
                     /gene="acm"
                     /locus_tag="IWO35_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ0317715.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPGATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFL"
BASE COUNT          847 a          348 c          423 g          546 t
ORIGIN      
        1 taggggagat agaaaaaatg aaaaagtgta gaaatattat tttatcaatg ttatttatta
       61 ttactaatat aacatcactg attcctgttc atgtctatgc ggatgcaggc agagatatca
      121 gcagtaatgt cacttcgttg accgtggacc caacgaatat tacagatgga ggaaacataa
      181 aagtaaaatt ctcgtttgac gaaaaaaaac aaaatatcca accaggtgat tatctttgga
      241 tcaattggcc aagtgaagga aatattcgcg gtgaaggatt ccaaaaagaa atcccattaa
      301 tgattgaaaa taaaaatgtt ggaactttaa cggtcagaaa agattctgcg caggttgtgt
      361 ttaatgaaaa tattaaaaac ctagactcag tagaaggttg ggggcaattt gagatacagg
      421 ccagaaacgt aaccgatacc ggtgaagaaa atacgggacc attcaccgtg acgagcggtg
      481 ataaaacagc tactgtaaat gtgacaaagc cagcttctgg ttcttcttct agcgtttttt
      541 attataaaac aggagacatg ctacctgaag atactaagca cattcgatgg tttttgaata
      601 ttaacaataa cggcacttat gttgaacaac cagttaaaat cagtgatgaa atccaaagtg
      661 gacaaagatt agatccaagt acgtttgaaa ttaatcaaat acacttaggt gaacagaagg
      721 tttatagagg agaagaggga atccaacaat ttttacaaga tttccccggc gctacattta
      781 atttttcagt aactgataat tacattgaaa taactatacc aaaaaacttt gtcaatctta
      841 ggaaaataat ggtgagttat aagacgatca tagaaaaccc agaacaaata aattttgaga
      901 atcattcgga agcttggttt aaagaattta ataaacctgc tgttgatgga gaaagtttca
      961 atcatacagt taaaaatatt tcagcttctg gtggagtcaa cggtacagtt agaggcgaat
     1021 tgaagatttt caaatatatc aatgacacag agattggtat cccaaatgtg actttcgaat
     1081 taagacgagc agatgaacag cctattcaag gacagtctag tatcctgttg acttcgaatg
     1141 aacagggaga agctagtatc aaaggcctgc aagtaggaga ctatgttgta aaagaaaaag
     1201 aagcacccaa ttggattgat tttgatccac taagttcaaa tgaactcaaa ttctccataa
     1261 acgaaaatga tacagaggga gtcagtttac ctatttataa caagaaaaag gttacgaaca
     1321 tcacagcaac taaaatttgg aacggtggaa ccactccaag accgagtatt tattttaaat
     1381 tattcagagc aagtacaaac aattgggaac cagttcctga tgcagaaaca aaacgattag
     1441 acaatggaat aacttcagtg acttgggaag atatccaaca gtatgatgat agtggaaacg
     1501 aatatacgtt taaagtgcaa gaagtagatc aaaacggaaa tgattatgtc ccttcaggtt
     1561 atcaaaaaat tgaaaacggg ctagtcgtta caaatgagaa taaagaagtc atttcagtct
     1621 caggacaaaa aacatgggaa gacaatgaga accaagacgg caaacgaccg acgacgatca
     1681 cagtaaatct attagccgac ggtcaaccga tccaacacaa agaagtaagt gaaaaagatg
     1741 attggcgcta tcgtttcaca aatctgccaa aatacagaga cgggcaagaa atcatctata
     1801 ctgtaacgga agacaacgtg cccgaataca gtacgacgat tgacgggtat aacatcaaaa
     1861 acagttatac accggaaaaa acaaatatat ctataccagg tagccatata acaccgtgga
     1921 aaaattttcc agagaagaca gagagagaga aaattagaaa tctattctct caaaaattgc
     1981 aaatatttgc tcatacagga gaatccagcg aacaaagaag caattatatt agtaagagag
     2041 ctgtacctaa acagataaca aataaatcaa aaagcattct tcctaaaaca agtagtaaaa
     2101 aatctagttt tgctgtaatc attggtttat tgatagtaac tatctgtgct gggatattct
     2161 taca
//
LOCUS       DACECZ010000204         2144 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.214, whole genome shotgun sequence.
ACCESSION   DACECZ010000204 DACECZ010000000
VERSION     DACECZ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.214"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(117..707)
                     /locus_tag="IWO35_002697"
     CDS             complement(117..707)
                     /locus_tag="IWO35_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ0317716.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1230..1391
                     /locus_tag="IWO35_002698"
     CDS             1230..1391
                     /locus_tag="IWO35_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317717.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1410..1904
                     /locus_tag="IWO35_002699"
     CDS             1410..1904
                     /locus_tag="IWO35_002699"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin"
                     /protein_id="HAQ0317718.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
BASE COUNT          774 a          332 c          364 g          674 t
ORIGIN      
        1 atgaatataa atagagtgta tcttctttga taaatgtcag aaaaagtgtg acaaataacc
       61 atcagtaaaa ttacttgtca tactattgat aaggagtatt taattgaaga gaatgattag
      121 gagaaaggat ctccgtactt tttatagtaa tcatagaatc caagtttgta tagacgaatc
      181 tttttttctc ttatttcttc tgtttcaaaa tggactgctt gaagaatcaa gtctgtgaat
      241 tctataggag ctgttccatt cgcagtaatc aaattacgat ctttcacagc ttgttgttct
      301 ataaaatctt ctttattctg ataattttcc gattctaacc aaggaaattg cgcatttccg
      361 gtgtgtttgt aaccttgcaa taatccgctc tgagctaaat aatcaactgc cccacaaata
      421 gcagctactg gtacgtctgt ttttaacaat ttatcgatta tttgatataa atgatgattt
      481 tttatgtccc atgaattgcc accgatcata ataaaaaggt caactttttc aggtatttgt
      541 tcgattaagt aatcgatttt tgtttttaat cctccaatgg aagtgacctc tgattggata
      601 gaagcagttt tgttttgcca tccctctttt tggtttagtg tgctagtaat ataagcacct
      661 tcccaatcgg catattcctc taatagaaac gtaatcgcag ttttcatttt caagctctcc
      721 tttactatct atgacaagga caacagtttt aacatacaga cattcattaa atttggatag
      781 cgacagcgaa aacaacaatt cttaagtttt agacagagta tacagacagt caacttaatt
      841 aaatacgaac taaataaaaa tgacaggtaa tcatttttgt ttaccagcat tataacattt
      901 tgcatacatg gatgacaaaa gaaccaattc ccacttgctt gagtacaatc ttaaaagaaa
      961 ctggaaggca aaaagtaatt atagaaacga aaaataaatc atatcgggca ctcagacatg
     1021 aaaacaaccc tagaaatata taactagtcc aatgaaaaaa aagcgaatgc cgtagaaaac
     1081 aaggattctg cgtacattca tatctcattt tgcgagcata tctgctatta ctgtgactaa
     1141 atgttaatat ggttaaaatg accgttatat tttttatata aatatcgtat aattagtatg
     1201 aatatagaag aaggaaagaa ggtgcaatct tggaacctaa agacaagtat ttagattacg
     1261 ctagggtcaa taaagataaa tttattgaaa caatcatcaa gggaaaaatc cctgatgatg
     1321 aaaaagatgc ggtattcatg gctggaagcc caggagcagg taaaacagag gttgcactag
     1381 gtttagctta aaattatgac aaccatgttg tgattgatgc ggactatttt agagcacaat
     1441 ttccagaata caatggaaag aactcaagtg tttttcagaa agcttcttct tggttagttg
     1501 aacaatcatt aaaatatgta ctggaacatg gctattcttt catactagag gatacttttg
     1561 ctatccttag tgcagagaaa aatattattc gagcccttaa aaataacttt cgcgttacga
     1621 ttttctatgt ttaccaagat cccaaagttg cctgggattt cactcgaaaa cgtgaactag
     1681 ctgaaggacg tcatgtgcca aaagaaatgt ttatcaatgc gttttttaag tccagagaga
     1741 atattgaaaa agtcaaaaat agacatcctg aagtagtcct tcacataatg attaaggatt
     1801 atcaaaacga catttctgaa gtgcattatg cagcagataa tattcaatta gtactaccaa
     1861 tacaatatac ttcaaaagag ttggaggagg aattacatga ctgagaaaga acaagtacaa
     1921 caaatcgtga agaagtataa taaaagtatc gctgatttat cagaaaacgc ctcagcgaaa
     1981 gaatttaaaa cagtaatgaa ttacgtagca gatgaagcaa atcgtaagca gcgaaaacta
     2041 gttgggttgg ataaataaat tttttaaacc atatccttgt aaagttgagt tttttatttt
     2101 aatgaattaa gactattaaa tagataatta ttgtaactta aaaa
//
LOCUS       DACECZ010000205         2119 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.215, whole genome shotgun sequence.
ACCESSION   DACECZ010000205 DACECZ010000000
VERSION     DACECZ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.215"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(580..924)
                     /locus_tag="IWO35_002700"
     CDS             complement(580..924)
                     /locus_tag="IWO35_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317719.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(1021..1266)
                     /locus_tag="IWO35_002701"
     CDS             complement(1021..1266)
                     /locus_tag="IWO35_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0317720.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1336..1590)
                     /locus_tag="IWO35_002702"
     CDS             complement(1336..1590)
                     /locus_tag="IWO35_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317721.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1754..2077)
                     /locus_tag="IWO35_002703"
     CDS             complement(1754..2077)
                     /locus_tag="IWO35_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317722.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          792 a          361 c          371 g          595 t
ORIGIN      
        1 gaatatttag aaattattaa ttttcgtctg aaaacaaaaa aagaaagcgt tattatttta
       61 gtctattttc agggggggaa ccttatgata aaaaaactat ttttactatc aatattaacg
      121 ctagcaatgg caggttgttc tgactctggc ttagcaactg aactgatcac aaaatcaact
      181 gaagccacga cagaggagtt aaaatgtgga aacgatcaca gtgactacag aggcttaaat
      241 cgacgatgca tgggaatatt aggcagaagc aattgtaagc atcgatgtag taatcgaaga
      301 aggtgtagaa gaagcaacag aaaaatcaaa agaaagatca aaagaagcaa tctgtgctgt
      361 gcatcgtata cagacgtcga agatgcggta cttttaaacc acgtcaggag atgttcattt
      421 atgtctaggg aagttggcga tgaaccaaaa ctagagtacg cttttagttt tgcacgtgag
      481 gatgaagata accctaaaac ttacgacatt ttagcttgat ctaagatggt taaagctttt
      541 taatgaagta agaggagata gaaggctttt ctagttatat taggtaagat aaattccttt
      601 ttgctccaaa tttgctaaac attctgctag ataaacttca tcgtgttccg gataaatcgg
      661 cacgttgcgc tctacagtag gtaaatcaaa aaatgcttcg catgatttgc cacgatacat
      721 aggatttttc cactctggtg ctgggctgta accacgtttt tccatttcat ccatgacaag
      781 ctgatgatat tggaataatc tataaggaga atagtgaaaa acataatcca cagtagcatg
      841 tttctttccc cagccatttc ctcttaatgc acagcattcc cgatgttgtc ctaaaagctg
      901 ttgacgagga agctttgaaa tcaatttttc atgccataat cacatagaaa tgtctccttt
      961 atatatagat tgttaacctt agacgttaaa tgaaaaaacc agcaccaagg ctggaaaaaa
     1021 tcacttcagt ttgcttgctg tttctgagcg accaattagg taatcaacgg aaatatcgta
     1081 aaagtcagcg atatagatta attcaatgat gcttggaatt tcttctcctt cttcgtactt
     1141 acgatatttg aataaaggaa tacttaacgc gctagaaaca gctacttgag ataaagatcg
     1201 gccagttctt aagaaattca gacgacaacg taaagtatcc agtaaaatag cttcttcgat
     1261 actcatttaa tcacctttca ttatagtaga atagttataa tgactataga aagctacaca
     1321 tgcaatcaat agtactcaat gttttttatt taaacgtatc aaataaagaa tgacgatcaa
     1381 taaagacaaa accagtcctg ataattcttc tataaaaaca ctatcgggtt caaagtagtt
     1441 gtaattaaaa actttccaca taaagtagat agccggaaga gtcaaaatag ctaaataaaa
     1501 ccgttctttg ttccaattca taagataaac aatacttgcg accagtgcga ccgcaggcac
     1561 ccaaaacaaa actctcacca acgtaatcat gtaacggact cctcaacgat tcatataata
     1621 aaagataaca tgttattgca tacatcacaa atgaaatgct aggaaaataa aagaagaata
     1681 gatcaatgaa aaatgaaaaa gcagaacagt tttctgctga taagcaggaa ctgttccgct
     1741 gaatgtgtct tatctatcga gaaaaaatca atggattgat atctaagtaa ttcaatacat
     1801 ataaaaagag gacgatcgca ttcactaaaa aggaaagaat gatgatccca tagcgaatgg
     1861 aaacatattt aacaaatact tttgtttgaa tacgggcata tctttttcct ttatagctga
     1921 gcatcatgac aagaagcaaa agggtaatca caccttgaaa aatcattcca ataaacagaa
     1981 ttcctgaggt ttgataattt aattggagaa aataagctgc tgccgcaact aaatcgaata
     2041 aaatcgcaat aaatggcaaa ataatcactc ctaacattga gaataaataa aagaataaca
     2101 ttttttctaa aataagcaa
//
LOCUS       DACECZ010000206         2104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.216, whole genome shotgun sequence.
ACCESSION   DACECZ010000206 DACECZ010000000
VERSION     DACECZ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.216"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            169..1539
                     /locus_tag="IWO35_002704"
     CDS             169..1539
                     /locus_tag="IWO35_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAQ0317723.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="IWO35_002705"
     CDS             1560..1844
                     /locus_tag="IWO35_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317724.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          731 a          308 c          444 g          621 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 cata
//
LOCUS       DACECZ010000207         2068 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.217, whole genome shotgun sequence.
ACCESSION   DACECZ010000207 DACECZ010000000
VERSION     DACECZ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.217"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..153
                     /locus_tag="IWO35_002706"
     CDS             <1..153
                     /locus_tag="IWO35_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0317725.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="IWO35_002707"
     CDS             159..575
                     /locus_tag="IWO35_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317726.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="IWO35_002708"
     CDS             681..944
                     /locus_tag="IWO35_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0317727.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..1216
                     /locus_tag="IWO35_002709"
     CDS             944..1216
                     /locus_tag="IWO35_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0317728.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            1293..1622
                     /locus_tag="IWO35_002710"
     CDS             1293..1622
                     /locus_tag="IWO35_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0317729.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1612..1899
                     /locus_tag="IWO35_002711"
     CDS             1612..1899
                     /locus_tag="IWO35_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0317730.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT          776 a          260 c          401 g          631 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga tttgaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacat atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggtagatg aagatgaaca cgtggttgaa atttattcag caaggacgca
     1201 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
     1261 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
     1321 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
     1381 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
     1441 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
     1501 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
     1561 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
     1621 gaaatcgttc cttcaaaaca tgtcattaag tatttaaaaa agctaaaaga gaagccattg
     1681 aaagaaaaat ttttgaattt aatttatgat gaaattgcga ttgatccata taagggtgaa
     1741 caaaaaactg gggatttatc aggtatttgg tctagtgggt tcaaatatgc gggcacaaca
     1801 tatagagtag cttacgagat tatagaaaac aaggttattc cagtattgtt atgtggaacg
     1861 catgaaaact tttatgaaca gttgaaaaaa ttaatctagt gatttgttgt cgttcttaag
     1921 aaggacggct tcttttttat cgaaaatctg attcgtctac atgactaaag tcgcgagtat
     1981 tctctgcttc tcgataaatt aaataacgtc attactttga ggaaagctat aaatcaatat
     2041 atttcaactt ttccttattt atgtacta
//
LOCUS       DACECZ010000208         2055 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.218, whole genome shotgun sequence.
ACCESSION   DACECZ010000208 DACECZ010000000
VERSION     DACECZ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.218"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            639..1157
                     /locus_tag="IWO35_002712"
     CDS             639..1157
                     /locus_tag="IWO35_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ0317731.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          710 a          294 c          393 g          658 t
ORIGIN      
        1 tatttttttg agttataata attcatgaag agaggtaatt cacttattta cccaagctga
       61 aaagcctgat attacttctt ttcaggcagt gctgctaata ttcaattaca cttttttgta
      121 taaatttagt tgttttaaat ttaattgtct gctatgcttg tgatgaattg attttcgttc
      181 tgcacttttg gacgataaat ttggacaatt gaaaatacaa aaaaaaagaa aggccctgtg
      241 agactcggaa ggctaccaac cttcgtcgac gtccacaggt ggcattaaga tcactccctt
      301 aatgcgttta cctttcagtg ttacggtatt acccgtaacg gatttagtat aacagatatt
      361 caaaagtgat accacttttt ttggctgtgt taaggttaaa aatacgtgat attacgatga
      421 atctatgcct aagcataatg ttataaaaat gtccgtgtgg ctctttgccg tatgacgaac
      481 gctgaaatta ggagacgttg ggatatttta taaatcattc acatatgagt tagacagtca
      541 atgcatatca gatatgcatt gtagaacgga ttaaaatatc gtggatgaaa ttatagatga
      601 attaaaggag acaattagga atttatggag gtaatataat gagaacatat aaaaataaag
      661 aagaattaaa aaaagaaata aataggactt atactaaata tattttagaa tttgaaacta
      721 ttcctgaaga attgaaggat aaaagagttg aggaagtgga tagaacacct gctgaaaatt
      781 tagcatatca agttggttgg acgaatttaa ttctaaaatg ggaaaatgat gaaagaaatg
      841 gattatctgt taaaacgcct tctgatcaat ttaaatggaa tcagttagga gagttatata
      901 agtggtttac aaatacctat gcacatctac cattgaaaga attagaggaa attctcaatc
      961 gtaatattga tgatattaac atgatgattg attcaatgag tgatgaagat ctattcactg
     1021 cacacaaaag aaaatgggca gacgaggcaa ccaaaacggc tgtatgggaa gtatataaat
     1081 ttattcatgt aaatacagtt gctccatttg ggacttttag aacaaagata agaaaatgga
     1141 agagattagc tttgtaaatt cccgtttact gctaagcaat tgtggaaagg agacctcttg
     1201 tatgttaaca gtaagaacat acgactacat aagttctttg ctaactgccg gtttttattg
     1261 ggtgaatgca agtgcactcc cctagaatgc cgtagagggt gcatcgttag attgtatcaa
     1321 ttttggttgg ccaacagata aaccatgact cacttcgata aaatgagatt atggtgccat
     1381 atccgctaca aagggactta ggagactagc aggggaaaga tttttttatt tttgggatgt
     1441 ttttcagacc gccgatagta ccgacaattc cctttaaata tggaatctca acaacccaca
     1501 tgaagggtaa ggcaagtccg gtaattatat cttctgactt tgggttagga agaattgaaa
     1561 ttgttaagaa cgattcaacg cttatggttt ataatttgga gatagccttt gttaatatgc
     1621 taaagccaac atacgataca gattttgagc agtttattct aaccatcaaa gggtatgtgg
     1681 cctatcgcct atcctattac tgatactgaa gagatcttaa ataaattcta tacgggattt
     1741 gaagatcctg aaggtgaaat cgtcatttat cagaataaat aaatcaaata gtagaagttc
     1801 aaagaatgag gggaacatta gtttttattg cttagtttaa tcccagtacc gatttctgca
     1861 agatgtaaaa ggtctttcat ttcataagtt ttcagaagca agttcatacg cactaactca
     1921 ctttcgattg agtagcaatc gtcaacaaac tttactatca gctgttattt ttattctttc
     1981 gtgttatcta aataccatca atttataagt tgtaggtgta tttaataaaa gaacaaataa
     2041 ctttagtatt tttat
//
LOCUS       DACECZ010000209         2043 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.219, whole genome shotgun sequence.
ACCESSION   DACECZ010000209 DACECZ010000000
VERSION     DACECZ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.219"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            273..890
                     /locus_tag="IWO35_002713"
     CDS             273..890
                     /locus_tag="IWO35_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0317732.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            931..1230
                     /locus_tag="IWO35_002714"
     CDS             931..1230
                     /locus_tag="IWO35_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317733.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1264..1431
                     /locus_tag="IWO35_002715"
     CDS             1264..1431
                     /locus_tag="IWO35_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0317734.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1476..2039)
                     /locus_tag="IWO35_002716"
     CDS             complement(1476..2039)
                     /locus_tag="IWO35_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0317735.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          670 a          335 c          398 g          640 t
ORIGIN      
        1 taaaatatac catggaagac ataaaagaaa aactggataa ctagtttatt taatggcaga
       61 ccgttaaggc ctgccatttt tatgtatttt agcggaataa atataatcat gcgggagtgt
      121 aaacttactg tttcaaaaag tatacttttt gacatgcctt gattttcttt gtttatgtat
      181 ggcataataa ccacataata caatttgaaa cgtttctgat ttaatctgat tgttttgaaa
      241 caattttagt gaaaaaaggg tagataaaaa cgatgagaaa atttttttat atccgggtca
      301 gtagttatga tcaatcgatc gaacgacaac tgatttccgc gaaggaatta ggaatccctg
      361 aagaatatat atttgtagaa aaagccagcg gcaaagattt taaacgccct gaatatcaat
      421 taatgaaacg gatgtttcga aaaggagatt tactctatct acaatcatta gatagactag
      481 gcagaaataa acaaatgatt ttagaagaat ggcgagaatt aactaaggaa aagcagattg
      541 atatttctgt gttagacatg cctctgttag atactgctag ttacaagcaa ctagatgggt
      601 tagagacgct tatctctgac ttaattctgc aattactcag ctatatggcc gaagacgagc
      661 ggaaacgaat taatagccgg caaagagaag gcgtagaagc tgcgaaacaa aagggtgtaa
      721 aattcggcag aaagaaaatt gctttacctc ctgaatttcc tgcgatctat tctgactgga
      781 aagcaggaaa gataactgca gtctatgcta tggatcaatt gggcatgaaa cgaaatacct
      841 tttatcgacg ggtgaaagaa tatgaacata ccttgcacca cttttcttga tagatataac
      901 agctattaga cgggaaggga gagtttgaaa atggacttaa cgaatcaaag ggtcgttttt
      961 actggaacgc tgcagcaaat gacacgaacc caagccatcg gtttggttca ctctttaaac
     1021 ggccattgtc aatcaagtgt tacttcaaaa acaaatttcc tggtttgtgg tcagtattct
     1081 aagtcgttgt ttgatgattc tctctataca aaaaaggaac aaaccgcgcg ggacttgatt
     1141 gcgttaggtc atgagattac cattttatca gaagtcgaat tttatgcgtt aattagtcaa
     1201 cagttgaaag aatggcaacg gtatttataa tcgttagtaa aatttacaag gaggaaaatt
     1261 tttatgaagc gaaaatctta tgatcgttgg ggcattgaaa taactagttt taatcgaaat
     1321 attgcttttc acgtggagtg tgactgtggg gaattagcaa aaagtgttta tcaaaaaaat
     1381 tacttttact gtcctaaatg ccatcgccga tacggggtac atggaacata gaaacgaaat
     1441 agtgcggtag aaaagcgaat gaatgtagta tatttctaca aatcattcgc tttctttatt
     1501 ttataaatag ttggtcgaga gatcccgttt tcttttgcaa tctctgcaac tggaatgcct
     1561 tgctttaaca tgtccaccac ggttttaaaa actaagcgtt tttgcggatc tgttgagtta
     1621 atagaatact cgattggtcg tcccttatat cggccggctt tttgagccaa taatactcct
     1681 tgccgctgac gttcacgaat tttctcacgc tcgttttgtg caatataact tagcagtgat
     1741 aaaaacatat cgaacatagc ggtatccagg agttcatttc cagtattaaa atctaaaaac
     1801 tgtgcatcta atatttttaa cgctactttt ttctgtttca taaaagcaac tgtatttttt
     1861 atatcttcat aatctcttcc taaacgatct agtgatgtaa cgatgacgca atcaccttca
     1921 cggatataat ttagtaattt ttgaaactca ggacgctcag tatctttacc actggctttt
     1981 tcttggaata atttatcaat gttaagtcct tcggttctgt tgcgaagttt gaaaatcaaa
     2041 cgc
//
LOCUS       DACECZ010000210         2013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.220, whole genome shotgun sequence.
ACCESSION   DACECZ010000210 DACECZ010000000
VERSION     DACECZ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.220"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            627..959
                     /locus_tag="IWO35_002717"
     CDS             627..959
                     /locus_tag="IWO35_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ0317736.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1578..1919
                     /locus_tag="IWO35_002718"
     CDS             1578..1919
                     /locus_tag="IWO35_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ0317737.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          698 a          271 c          427 g          617 t
ORIGIN      
        1 gaaaagggaa cgtatgtttg catactcctt ttttttgagt tataataata cgcgaagaga
       61 gataatttca ttatttaaac aagctaaaaa tcttgatatt acttcttttc aggcaatacg
      121 aatattattc gcttacattt tttgtataaa tttaattgtc tgctatgctt gtgatgaatt
      181 gattttcgtt ctgcactttt ggatgataaa tttggacaat tgaaaataca aaaaaaagaa
      241 aggcggtatg aactcggaag gctggtaacc ttcgtcagcg ttcatacacg gcattaagat
      301 ttggccctta atgcgttaac ctttcagtgt tacggtatta cccgcaatgg atttagtata
      361 acagatattc aaaagtgata ccactttttt tggctgtgtt aaggttaaaa atacgtgata
      421 ttacgatgaa tctatgccta agcacaatgt tataaaaatg tccgtgtggc tctttgccgt
      481 atgacgaacg ctgaaattag agggaacgca tgtttggatg cgttccctct tttttgtttg
      541 tcatgaacga aaatcaattc tctatttttg aaaaataaca agaaaaactt gttaaaaata
      601 tttaaagaaa ttgggtggtt tattttatgt catatacagg aattttgagt ctcaaagata
      661 tttgtcacta cggtaaacgg tgtaccgcta cagagaaaat cacgaagaaa ctatcaactg
      721 gccaaagtaa aaccgttgtg cagtgtaaga aatatataat ccagaaagac aaagtctcag
      781 aggaaatgat ttattacatt gggaaacaaa agcaaataat tttaaaagat cctatcccct
      841 taaaagagct atatccaaca atcaaacatg tttatgacca aaacggtgta ctgattggtc
      901 gaagaaaaaa tggagtcttg cgttgtaccg caaaagggat ggggcgtttg atcggctgaa
      961 ttgatcattc tcataacttg tattttatcg tttttcgctt gcttgaaaat attgtcgaga
     1021 cttctaaagg aacttagagt cactagaaaa ataaggagta gacttgatct atcaacaatt
     1081 cccttcgggg agtgacgggg taggttttaa cgattggtaa cgtaaaaaat tggtcaatcg
     1141 agtaaatgcg cttgtttgga tagtggtgaa atgaatttgt gtaaataata agcgcagagt
     1201 aaacaagagg gaatttagaa gttagacaag ttagaatagc gtctagatta atcatgagca
     1261 gctactaagg gatctagact ttgacgcaaa ctcatggaat aaggagtagg tcttctcgta
     1321 atggggagga cgcacaagaa attgtgtcgt gtgctgcagt acatgtagat acttgcgagg
     1381 acgttgatta ttggatcaat cgagcattta ccgctgtcta ctagggtttc tcgtttcgga
     1441 cgccataaac gagtcgaaat tttttatgta tttttaagta aacgaaaaac gataaaatat
     1501 gaacaagtcc tgaataaagt gaaaaaattg atataaaaca tagagaaaat gaaatttacg
     1561 taatggagga atgacaaatg aaaaattatg atccgaatat acgattgggc acacatacta
     1621 ttaaggtttc tttccaacgt tgggattaca aaggatttgt gacgtttaga agaggcggaa
     1681 attgtaaagg attagatgtt ttagcattag acgaggatga tttatacgat caaacattga
     1741 ctgataatcc aattggattc ggcttgctac ctgaagatga tgaaggtaat gaatggttta
     1801 aaatgacgct tatgaatgat aatggagacg aattatctgt tgaagatact tggtcatatt
     1861 tgagtgatta tattgtcagc tttgaaatca ttgaatttgt agcggataaa gaagaataaa
     1921 aactggagga ataaatttta cagataaagg cttagaagga gtcacgatga ttaataaaaa
     1981 ggtagtatta ccttaggcat tgagtaagga att
//
LOCUS       DACECZ010000211         2011 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.221, whole genome shotgun sequence.
ACCESSION   DACECZ010000211 DACECZ010000000
VERSION     DACECZ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.221"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..425)
                     /locus_tag="IWO35_002719"
     CDS             complement(<1..425)
                     /locus_tag="IWO35_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ0317738.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="IWO35_002720"
     CDS             complement(534..1565)
                     /locus_tag="IWO35_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0317739.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          622 a          328 c          348 g          713 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa
     1681 gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct
     1741 tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt
     1801 ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga aaacattttt
     1861 tcagaatcga ttggctctcc atttattcaa taatggtttg tctaacgata ttgttgggat
     1921 aattcttaaa gaagtattag aaatagttgg tacttcatta ctttacatga agatttattc
     1981 tatggtagga gcaattatat tgagaattaa t
//
LOCUS       DACECZ010000212         1987 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.222, whole genome shotgun sequence.
ACCESSION   DACECZ010000212 DACECZ010000000
VERSION     DACECZ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.222"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            155..826
                     /locus_tag="IWO35_002721"
     CDS             155..826
                     /locus_tag="IWO35_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0317740.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(1551..1886)
                     /locus_tag="IWO35_002722"
                     /pseudo
     CDS             complement(1551..1886)
                     /locus_tag="IWO35_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          718 a          313 c          253 g          703 t
ORIGIN      
        1 ccttcttttt ggactgattt tatcgaaact tattttttct ttttttcatg tattctttta
       61 tttttataac tagtgtatta cttcaaaatt tgatattata tggcatgaac agaattaaaa
      121 tatcagttaa aataaaagtt aaggagcatt gtgtatgaca attaaaacta gtctccaaaa
      181 tcaagcttac caatctattc gccgacaaat tatctatgct gacttagaac ctggaaaaaa
      241 agtatcagaa aaaaaattag aagaaacact ttctattggt cgtacaccaa tacgtgaagc
      301 actgattcaa ctccgacaac aaggtcttgt tgatacaatt cctcaaagcg gaaattatat
      361 ctcattaatt gatttagatt cggccaaaaa tgctcgattt gtcagagaac atttggaacg
      421 acaaattatg ttagagtgtt gtgccaaaat gaataaccaa aaaaaacgga ttttagaaac
      481 tataatttca gaacaagaaa aagctgttag tcaaaaaaat gctcgtagtt tttttcataa
      541 tgataattta ttccataaag cttgttttga aattgcagag cgagaagaag tatggcaatg
      601 gttagaaaat aacaatacac acttagaacg atttcgttgg ctacgagtaa aaacggaagg
      661 attaaagtgg gaaaaaatta tggaacaaca ttatcaacta tttgaggcat taacaaaaca
      721 agatccagaa gaagctaact ttctaacatc gttgcatctt catttaatgc ttaatgagca
      781 aaatattgtt ttagctactc atccaaaata tttcaaaaat atataacttt taactctttt
      841 taaattaaat ggttgttctg atcagaaaaa tatcgcttgt tttcttatta tgtaatatat
      901 ttcataaact attctgcata acaactattg tttgtacttt ttacattgga taactaatta
      961 tgactgctga taacttttga tattaaacta gtatctccaa tacaaataaa aaatagatat
     1021 cttgttcatc aaaattagtt cgtggatact taccttcatt tacatcattc agcggtttcc
     1081 cattttcttt tattctatta tttcattttc taatagactt ggcttcattt cgcaatactt
     1141 tttttcaata ggcatcaaat tggtgttgtt tagatattta gttgtagttt tgtgacatga
     1201 taagtatgct cctttttctc ttttccacta atacaacgat caaagggtgc ttttttcaaa
     1261 aaactagaaa aatcaaaata agattcgtat tttgattcta tatatcctgt ttcaactaca
     1321 tcttatattt cacaatagaa taaatagcct tttgcatagc tgcttaactt ataagaaatt
     1381 gcaatttact tcttcaaagt gaaaacgaat atgagtaaaa taaaattatt cttcttacag
     1441 ataattaaaa atagcgcgta acaaaaatcg gcattcaatc acataattag gattgatgcc
     1501 gattacttat attctacgta tagttttagt attcttattt tttatcacca gtaaccaatt
     1561 gagaacagcc ctaaagcaga atagtctttt atctcaaaac gcttaatttc aggcttagga
     1621 atatgaataa tcttttgagt atattctttc tcttttaatc ttactgaaga aataccactg
     1681 cctatctttt ttacaaatat ttcatttggt gtacagacta ataaaacaat atttgtaatc
     1741 aatccttgca ctaaagaatt aaaaggaaga ctatttaaaa cagggtgtgc ataactataa
     1801 catgtgaagt agttattttg aactttaact tttaaaaact ttaaaaataa tcttatttgt
     1861 tcttctaaac aatcatctat actcatataa tcactccact aattaatctc cccaaaaaga
     1921 cacagcatta agagatatac caacacaaca gtgcaaataa ctctacatct tccttcgcaa
     1981 ccttatt
//
LOCUS       DACECZ010000213         1957 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.223, whole genome shotgun sequence.
ACCESSION   DACECZ010000213 DACECZ010000000
VERSION     DACECZ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.223"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(365..1657)
                     /locus_tag="IWO35_002723"
     CDS             complement(365..1657)
                     /locus_tag="IWO35_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0317741.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          621 a          384 c          319 g          633 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
     1681 ttgtagaaaa agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg
     1741 gtttatatta cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt
     1801 atctatttga aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatccta
     1861 ttatccatat ctttttcagt tttaatattg atacacatta taagaaaaag aaacttttgg
     1921 ttaatttatt tcatcccagt gcaaaatata aaaaagt
//
LOCUS       DACECZ010000214         1928 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.224, whole genome shotgun sequence.
ACCESSION   DACECZ010000214 DACECZ010000000
VERSION     DACECZ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.224"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..868
                     /locus_tag="IWO35_002724"
     CDS             <1..868
                     /locus_tag="IWO35_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ0317742.1"
                     /translation="NREANNIKKKMLIPLESEITGLLNKLTISNVDSTREKIIRTFDK
                     INKLFKNDEAEFKKELLELKLQELELNYSYIVKVNDEKEQQKAIKEQMIEEEKVRREI
                     EREKKRIDKEERQFNSEITKLMAYMQKASADVEKELYADKIKELEEKLRELETVKEDV
                     LQRELNTRAGYVYVISNIGSFGEDVYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMI
                     FSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKVSLDEIEKVVLENYNGTVSFTKLAQA
                     EQYRRSLELTDV"
     gene            934..>1928
                     /locus_tag="IWO35_002725"
     CDS             934..>1928
                     /locus_tag="IWO35_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0317743.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATK"
BASE COUNT          816 a          246 c          323 g          543 t
ORIGIN      
        1 taatagagaa gctaataata ttaaaaagaa aatgcttata ccattagaat cagaaataac
       61 tggacttctc aataaactta ctatttctaa cgtagattca actagagaaa aaattataag
      121 aacttttgat aaaataaaca agctttttaa aaacgatgaa gcagaattca aaaaagaatt
      181 acttgaatta aaacttcaag aattagaact caactattca tacatagtta aagttaacga
      241 tgaaaaagaa cagcaaaaag caataaaaga acaaatgatt gaagaagaaa aggtaagacg
      301 tgaaatagaa cgtgaaaaga aacgcattga taaagaagaa cgacagttta actcagagat
      361 aactaagtta atggcataca tgcaaaaagc tagtgctgat gttgaaaaag aactctatgc
      421 tgataaaatt aaagaactgg aagagaaatt aagagaacta gaaactgtca aagaagatgt
      481 attgcaaaga gaacttaata ctagagctgg ctatgtttat gtcatcagta atatcggatc
      541 tttcggtgaa gatgtctata aaataggaat gactagacgg ttagaaccta tggatagaat
      601 taaggaactt agtagcgctt ctgtaccttt tgaatttgat gttcatgcaa tgatattttc
      661 agaagatgca ccaacattag aaaatcaact acattcgcat tttagaaaaa acgaagtcaa
      721 taaagttaat caacgaaaag aattctttaa agtatctctt gatgaaattg aaaaagttgt
      781 tttagaaaat tataacggaa ctgtatcttt tactaaacta gctcaagctg aacaatacag
      841 aagatcatta gaattaacag atgtgtagta aaaagattag ccttcgggct tttcttttac
      901 aacgataaga acatacattc gaaaggagat aacatgaaac gagtagcatt atatatgaga
      961 gtatcaactg aacaacaagc caaacatgga gatagtctta gagaacaaaa agaaaccctc
     1021 tatgaatata tcgaacaaca caaggatttg aaagtagtta atgaatatgt agacggtggt
     1081 atttctggtc aaaaaataaa tcgtgatgaa tttcaaaaac tattacaaga tgtaaaagaa
     1141 aataaaatag atctaatttt atttacaaaa ttagatagat ggtttagaaa tctacgacat
     1201 tatttaaaca ctcaagaaat tttagaaaaa cacaatgttt cttggaatgc tgtttctcaa
     1261 caatattatg atacaactac agcgtatggc agaactttca ttgcacaagt aatgagtttt
     1321 gctgaattgg aagcccaaat tgattcagaa cgcatcaaag ctgttatggc aaataaaatt
     1381 gcacaaggtg aagtagtaag cgggaaaact ccgttaggct actctataga aaataagaaa
     1441 ttagtaataa atgatgatgc tcctatcgtg atagatatat tcaactactt tctttctagt
     1501 ggtagtttaa gaaaaactgt ttattattta ggctctcagt atggaatcgt tagagattat
     1561 caaagtgtta aaaatatgtt gacgaacaaa aaatacattg gtgaattaag aaataataaa
     1621 aattactgtc ctcctattat tgataaaaaa cttttttatg ctgttcaaaa agccttacct
     1681 aaaaatttaa aaacaaatgc aaaacgtgat tacattttta aaggtttatt aaaatgttct
     1741 gactgtcagg gatcagtagc tggtcaaact attaaagcaa gatataagaa aaaagacggt
     1801 acagaatcta tatacgaaag gacttgttat agatgtgtaa aacgaagaaa taataaatta
     1861 cgttgcacaa ataagcgcgc tttttacgaa aaaaatttag aacgttatat ttttgaagct
     1921 actaaaca
//
LOCUS       DACECZ010000215         1843 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.225, whole genome shotgun sequence.
ACCESSION   DACECZ010000215 DACECZ010000000
VERSION     DACECZ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.225"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            124..1737
                     /locus_tag="IWO35_002726"
     CDS             124..1737
                     /locus_tag="IWO35_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ0317744.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          584 a          332 c          385 g          542 t
ORIGIN      
        1 ttatccacaa attatttctc tatattttct gtctattcaa aataaacaat aattttccgg
       61 cacaattatt tgctgttttt taccataaca tagtacgatc aacgtagaaa aggagatgtt
      121 tttatgaaag ttgttgtcat tggcggtgta gctggcggtc cttcatttgc cactcgtttc
      181 cgtcgattga atgaagcgca cgaaatcatt atctatgaac gcggagaaaa tatttcttat
      241 gcaagttgtg ctttgcctta ttatttagga ggtgtgatca ctgaccgtga ctcgctgatc
      301 gaacgtacac cagaaatatt gaaaacaaaa aacaacatcg atgtctttac taaacacgaa
      361 gtaacagcaa tcgatccttc tactaagcga ttaacagtta aagacctatc cacaaatgaa
      421 gaaataaaaa cagattacga taagttgatc atctcttctg gtgctagacc agattatccg
      481 gatattcccg gagtttttga agcagaaaac ggctttgtcc tccgtagtgt gacggatgcg
      541 gatcgaatca aatcgttcct tgaagaaaaa aatccacaac atgtcgtcat tcttggtgca
      601 ggtgttatgg gtctggaatt agctgagaat ttcaagcatc gcggcttaga cgtgacttta
      661 atcgatcaat tgccacaagt cgctttccct tatgatccag aaattgctaa tttagtttat
      721 gacaaattgc tgaaagaagg attagccgtt catttagaaa caagagttac tgagatccgt
      781 gataaaggtc gagaaatagt attatcagat ggctccgtcc tttctgctga tatgctaatt
      841 tttgctgttg gtgtttctcc gaagaatgaa gtggtgaaag caaccggcat acaattatca
      901 gatacaggtc agatcattgt tgatgaccag ttacaaacca atctgccgga catctatgcg
      961 attggcgata ttatcgaaac gactagtgta gtgactggtc agccgatcca aagtatgctt
     1021 tccagtgcgg ccaatcgtca aggacatatg ctggcagata ttttaaatgg tacgcctatg
     1081 cgctatcgcg gatatattgg tgcaggtgtc gcaaaaatct ttgatcatac agcaagttat
     1141 gcgggaatga cagaacatga attaaaagca tcaggcataa caaattataa aactgttttt
     1201 atcactccct ttgaccatgc ctatttctat ccagaagcta caagattaaa tctaaagctg
     1261 atttttgatg cagatagcgg tcgtatttta ggtggacaag catttggaga aaaaggtgtc
     1321 gataaacgga tgggagaact ttctgtagca atcaccggaa acttgacagt ctttgatttg
     1381 cctgatttgg agttgcctta ctctccaccg tattctacta cccgtgaccc gttgaatata
     1441 gctggttatg tcgcaatcaa tcaaatgacg aatatcgtag aaacgatcaa agcaagtgat
     1501 atacccgaaa acgatttgaa agaagcgttc tttttagata tacgtgaacc taataaagca
     1561 ccttctggca gtatctcggc aacaaaaaat attccaatga atgagttacg agatcgaatc
     1621 aatgaaatcc caaaagataa aaaagtttac attactttca gaagaggatt gaatacttat
     1681 acttctgccc gaatcttggc aggtttgggt atcaaagcga ttttgattga agaataaaaa
     1741 aacgaccgcg gtgggtagcc gcggtctagg agttattttt acttgaggag taaaaatgaa
     1801 aaagtatata gggttgttgg ttggtatgta tatagaatag aag
//
LOCUS       DACECZ010000216         1805 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.226, whole genome shotgun sequence.
ACCESSION   DACECZ010000216 DACECZ010000000
VERSION     DACECZ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.226"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            198..794
                     /locus_tag="IWO35_002727"
     CDS             198..794
                     /locus_tag="IWO35_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0317745.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(852..1334)
                     /locus_tag="IWO35_002728"
     CDS             complement(852..1334)
                     /locus_tag="IWO35_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317746.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1440..1637)
                     /locus_tag="IWO35_002729"
     CDS             complement(1440..1637)
                     /locus_tag="IWO35_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317747.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          608 a          362 c          275 g          560 t
ORIGIN      
        1 aaaaattcct aaaacacaac aatgaaagcg ataactaaga aaatccttac ataacgattt
       61 ttcttattaa aattgttttt tgaacttcgc ttattttttg tgagcatatc tgtttactta
      121 ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa aaaaatgcaa aacgcaaatc
      181 aaaggagaaa ttttattatg acaactttta ctgacacttt gaaaaaccgc cgttcaatct
      241 atcatctagg acgtaatgtt tcactttcta atgaagaatt aacaactttg atcaaagaag
      301 caattaaaga aagcccaact gctttcaatg ctcaatcaac acgcgcagtt atccttttcg
      361 gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc acttcgccca ttgactccag
      421 cagaagcttt cccaaataca caaaacaaat tagctggttt caaaaatggt tatggtacag
      481 tattattctt caaagatact gacgtggtaa aaggtctaca agaacaattt gaattatatg
      541 cagacaactt cccagattgg tcagaacaat caaacggaat cgcaacagct aatacttggg
      601 tagcattagt tgataaagga ttgggcgcaa acttgcaaca ttacaaccca gttattgacg
      661 aagcagttgc aaaagaatgg aatatccctt caaactggaa actacgttcg caattagtat
      721 tcggttcacc agaaacaccg gctggggaaa aagaatacat gaacgatgcc gatcgcttcc
      781 gcgtatttgg ataattttat ttgaaataaa aaacagaggt tgtgacaaac atcttgtcgc
      841 aacctctgtt tttattctgg atcaatcata atatcaatct ctgcctcatc gtttaagcgt
      901 tgggacaaaa atggaactaa ttcttctaat gatgcagaaa atttcagcat cggtcctcgc
      961 catccgtttc ggaaaaaatt catttttacg attttagaac cttttggctg atataataca
     1021 attttattga tctcagaaaa aggaacacct tttttgtcaa agctattcaa aacaagtcct
     1081 tcttctgtga ttcctctact gtccaacaaa aaactaataa aaatcattgc tgacaagatc
     1141 ccttttgcca attcatctgc tgtaggatga ctgatgagtg tataccaaat gactagtgac
     1201 actaagaagg gtatcaccaa gtagacccat cgatttcgca cagtccgata aataatttct
     1261 tttcgaacca gccaaagaaa taaaaacatc acaacaacga ataaaaacaa tagaaccgta
     1321 aagaatgtta ccatgacaac tcgcctcgct ttttcttctc cagaaaatag gaaaagaata
     1381 ctgatttacc ttctcaaaga aattggactt gcagcacgcc gcacgtccaa ttgttttctt
     1441 tattcaaatg tatcatattt tgcaacaacc atgcttgctt tttcatctaa taattcttgg
     1501 tcctctgcag cacattgggc aactttgacc acggcgcagt ttgtcatttt tgatacaact
     1561 tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg catattgttc tcctaaatta
     1621 atcatttctt ccgtcatcaa aatcctccta aactgctgga acttttctcc acattaccac
     1681 tgctatttct ttggagattt aactcattga tttttattta cagttaaaaa tcaaccttac
     1741 aggcattata gcgtactgtt ttcgttttca caattaaaac agctgttgta tttagaagaa
     1801 ttttt
//
LOCUS       DACECZ010000217         1800 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.227, whole genome shotgun sequence.
ACCESSION   DACECZ010000217 DACECZ010000000
VERSION     DACECZ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.227"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            569..1381
                     /locus_tag="IWO35_002730"
     CDS             569..1381
                     /locus_tag="IWO35_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317748.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
BASE COUNT          612 a          254 c          299 g          635 t
ORIGIN      
        1 aaaagcagtt atttgttaaa atcattgata ttattaataa taagtatttt ttgaaagggg
       61 aaatataaaa ggtttttgaa aataaataaa aaatagtatt acttgttcta ttgctagtta
      121 ttgctagtct ttattctttt ttgtttttta gggatgtaca aaagatctca accacaggag
      181 acattgcttt tcacttttct agaataaaat gcttgtcctc agtattttct ggtccaataa
      241 attttaccac atttaatagc tatggcggag gcgtgaattt ttttatccat atctaacatc
      301 tttccagccg taatatttta ctggacaaca aacaatttga ttctaagtta tatacttact
      361 atacgttggg atactgaata tctgtacaat tctagtgagc tatcaatatg gacaaaagaa
      421 tagatgcagc cttcttattc agctgtttgt atagctttta tggattgata tatattagcg
      481 tactgcaatt tcagaatcaa ttgcgttgac gattgtacca gttgtcttgt attacacata
      541 tactcttctt tatgaaagaa aaaactgcgt gattctttta gctgtcagta tgagtttgct
      601 catctatacc catattctct ccacctttat gagtgtgatt gttattggct tcttgtttat
      661 cggctctctt ttctctaagc ggaaagagag aaaagaaatg tcgaatctga tatcaaatat
      721 gataaaagct gttggttgga ccattctttt aacagcttac gtttggtatc cgatgttaca
      781 acaagtccta catcaaaaaa ttaatcgtcc ttttcgcaca aaacttcaag aaagggcatt
      841 aaacatttct gatagtttaa tcggtgcagt aaataatgat ttagttacat ttacaatggg
      901 aacaattggt gtggtttcat ttattcttcc acttttcttt aaaaaaaaat ttgccaataa
      961 agaaaagatt attaattttt gtgcagtgac cacttggttc ttatcaacga atctttttcc
     1021 atgggaattt ttacaaaaaa cacctattca aatcattcag tttccttgga gaatcttggg
     1081 ttttcaagtg ctttttggtt cattgatctt aattatagtg tttttaaagt ggaaaacaag
     1141 caacaaaaaa agtatgtgta gccttgtagg aatagtcctt cttattttta cagtgactgt
     1201 agcaacagaa gcgaattatt cgcagaaaat tcaatcgtat aaaggtcgtt taattatgac
     1261 caaaaaagat gttacacttt atacaacctc gagaactgga ggtctttatg actatgcacc
     1321 attagatgca ttgaaatata aagatcattt aaagaaacat gaagtaaaag tctatgattg
     1381 atgggagaat gcatctcata aagcacataa ttctaaaata atctatacag taggagatgc
     1441 gaatgagcaa aatatcacgt tacctgtatt taattactgg gaacagttat gaaagtaaat
     1501 gggaaaaaga gcactataga aaatgacaat ggtttagtgt ctattaaagg tacgggaaaa
     1561 agaactatag ttgaggtttc atctacgtat ccaactacga tgattctatc tttattaatt
     1621 agttcaataa cttttatatt cttatttaca cgttatttaa taagatgctg cggttaaatg
     1681 aaaatagatt agatagatat tataaatatg ttgattctat tttcatagca atggagaaaa
     1741 tacattagca atatataaaa gaaggtatca agagtcacgg tgaatttatt ccgaaaagtg
//
LOCUS       DACECZ010000218         1795 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.228, whole genome shotgun sequence.
ACCESSION   DACECZ010000218 DACECZ010000000
VERSION     DACECZ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.228"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..761)
                     /locus_tag="IWO35_002731"
     CDS             complement(<1..761)
                     /locus_tag="IWO35_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAQ0317749.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYL"
     gene            complement(784..>1795)
                     /locus_tag="IWO35_002732"
     CDS             complement(784..>1795)
                     /locus_tag="IWO35_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HAQ0317750.1"
                     /translation="DLKDEDLLPATLSKELITDLLRGKLGFNGLVVTDASHMLGMTGA
                     MKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKGIITDDRLLDALKRILGLKAK
                     LGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQAITLVKDKQDIWPIVPEKYKR
                     ILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFEVEKYISPEEELKGLSQEEKI
                     KNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIVWPASKGTPDIPFYIHEIPTI
                     FVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLMGLSEFKGVSPIDAYCGLKDT
                     FI"
BASE COUNT          553 a          353 c          224 g          665 t
ORIGIN      
        1 tataaataag cgtctagagg ttcccaaaaa attggattct taagctgaat cattcctttc
       61 tttccttcag cttcagctac aagttgagcc tcttcgtcat atatcgctat taagattttt
      121 ccggtccatt ctgtatcgat attgaattct acacttgcat ccaaatcagt tattcttgta
      181 ttaatagtaa tatcacttat atagtctttc ggagttgtat ataacttaac aggtctatga
      241 atacctgcat aattgaagaa atcaaaatta actaagtttt ccttttttat atccccattt
      301 tcctgtactg tttccttcat aatccccaca ggtaaagtac tatcgtcaat aatattgttt
      361 acagcaactg taattcgatt attcccgtct tttacaaacc gattaatttc agactcaaat
      421 ggtgtaaacc cacctttgtg ttgagtaata aactttccat tcaaaaaaac ttttgcttca
      481 tgtgttgcag atccaaaacg aagaactaat ctttcttcta ataatctttt aggaagagtc
      541 acttcttttt cataccacac ccatccaaca tgatttctaa tgttctctgt ttcaaataaa
      601 tcattataag aagagggaac tgaaatcgga taactctctt tttcatttat tggttctaaa
      661 gcaagttctt cctgtaagtc atcatttgta tctaatttaa atctccagag accgcttaag
      721 tcaattactt gcctcgtctc tgtcataatg ggatacaaca tataaaccac tcctatctaa
      781 atattaaata aatgtatcct ttaaaccaca ataagcatct attggactta ctcccttaaa
      841 ttcagaaaga cccattagtt tgtccaccaa tatttctaaa gtttcttgtt ttccatcata
      901 agtattaata taagtcttta cttgaggtac atcagccaaa tgaaacggac actgtacaga
      961 tacaaaaatt gttggaattt catgaatgta aaacggtata tccggagttc ctttacttgc
     1021 tggccagaca attcgttgaa ctgtgcctgg attaacatta gctatattaa tgatcaagtc
     1081 atatttttta gttaaatttt taattggtct cttagcagca tatattccag ctatattttt
     1141 aattttttct tcttgcgata atcctttcaa ttcttcttca ggtgaaatgt atttttcaac
     1201 ttcaaatcct tttgctgaca aaatctgttt caataaatca atggcttgag gtctatttcc
     1261 agcaactact tcaccaaacc caccttttac cccatgtaca tctactagta aaatcctttt
     1321 atatttttct ggtacaatag gccaaatatc ttgtttatct ttaactaatg taattgcttg
     1381 gtcagccacc tctttaaaaa ttgatttatt ttcaattaaa ccaatttttt ccagagcttc
     1441 ttgcttaggt aataaaattt gttcttttgc ttttttgtgt aatcccaatt tagcttttaa
     1501 ccctaaaatt ctttttaagg catctaataa ccgatcatca gtgataattc ctttacgata
     1561 gccttccatc atataattaa agtcttcatc tggatcatta aaaaatagaa atagatcaca
     1621 tccagctgca atagaagtgg gcaataattc tgatcgtttc attgccccag tcattcctaa
     1681 catatgacta gcatcagtaa ctactaatcc attaaatccg agttttcctc taagtaagtc
     1741 agtgattaat tcttttgata aagttgcagg aagtaaatct tcatctttca gatca
//
LOCUS       DACECZ010000219         1763 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.229, whole genome shotgun sequence.
ACCESSION   DACECZ010000219 DACECZ010000000
VERSION     DACECZ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.229"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1016
                     /locus_tag="IWO35_002733"
     CDS             <1..1016
                     /locus_tag="IWO35_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ0317751.1"
                     /translation="LQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFMDNELT
                     DDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTNGSLVT
                     QDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEEVDVAF
                     CPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLVSDINY
                     IKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGNIKCHS
                     LSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILDEYKHD
                     ILI"
     gene            1072..1290
                     /locus_tag="IWO35_002734"
     CDS             1072..1290
                     /locus_tag="IWO35_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317752.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1361..>1763
                     /locus_tag="IWO35_002735"
     CDS             1361..>1763
                     /locus_tag="IWO35_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317753.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHP"
BASE COUNT          672 a          202 c          326 g          563 t
ORIGIN      
        1 aacttcagat gggtgaaatt gttagaggtc ctagtcaaat atctattgat gttacatata
       61 aatgtaattt tagatgtaaa cattgttaca atgcgagtgg tggagacaat tgttttatgg
      121 ataatgagtt gactgatgat gaactgttag aacttacaaa agatatatgt aatttaaagc
      181 catataatat ttgtttctgt ggaggagaaa ctttactgag aaaaaaaata gttttatctt
      241 gtgccacttt attaaaagag gcaggtattc ctaatattgc tattgttact aatggatcat
      301 tagtaactca ggatgttgca aaagaattat tcaaagcagg tattaatcga tttcaagtaa
      361 gtttagatgg aaaaaatgct agtagccatg aagcacttag attatatcca aacagttttg
      421 aaaaggcaat taatgctatc aaattattta gagaattgtc cgaagaagtg gatgttgctt
      481 tttgtcctac tagttttaat tacaatcaat tacgagaagt atatcaacta gtaaatactc
      541 ttaaagtgaa gtctttaaga attcaaccat ttatgcttag tggtagggga atgcaaaata
      601 gggaaatagt gccaactgtt tctcaatata gaaaactagt ttctgatatt aactatatta
      661 aaagaaatga agattatacc aagatagatt ggggagatcc aattgatcat atcatccgac
      721 acagaacttt attgaaagat ttttcgaatt ttgtttctat taggtcagac ggaggattaa
      781 tggcatcacc ttacttgcca attcaagttg gaaatattaa atgtcacagt ctttctaaat
      841 attggcgagc aggactttat aaagtttttt ctttagattt agttcaagat atagctactc
      901 aagtttcttc aatacaagat atgagtaaag aattaaaaaa aggacatatt aaatggctta
      961 atgaagatat agaaattgat attctcgatg aatacaaaca tgatatttta atttaggttt
     1021 atcattggaa agtttagata aacaaagtac ttttagaaag gagggaaaaa aatgatttct
     1081 gaacctcaaa tgaataatat gtatgaaagc ccaactataa tgggagctgg tgaagctgcg
     1141 ccaagagctt gttcagcaat tatggtgtgt gtgttattag catctgcagg agtaggtatc
     1201 caagcaggag cggtgtatac ttatgctgta gttgctacag tagctgtagc tgcagccgca
     1261 gtttggaaaa cagtaggatt agttggttag gaattaatag gagactaact gtctcctatt
     1321 gattccaatg attaagctaa ggaggaattt taagaaaata atgaaataca ataatttaaa
     1381 accgaaatta cttaatgaga acatagaata tataagagaa gaaaattatg gatatttaac
     1441 catagttccc aaaaatggaa ctgaattgaa agataacgtt ttcaatccaa cagtaagata
     1501 tatattggct aactgtactg gaaaaaacag tgtaaaaaaa atagcgaatg gtttaaaaaa
     1561 gaagtttcct agtttagaat atgaaaaatt ctatcaagat gttgtaggaa ctattaaatt
     1621 tttcaaacac tataatatta ttgatatagg agattatagt atggaagaaa agaaaatata
     1681 taaaattgaa caaaatttag aattagaatt actaaatgaa tcacattatg aagaaattta
     1741 taattttatc acagatcatc ctg
//
LOCUS       DACECZ010000220         1647 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.230, whole genome shotgun sequence.
ACCESSION   DACECZ010000220 DACECZ010000000
VERSION     DACECZ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.230"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(20..91)
                     /locus_tag="IWO35_002736"
     tRNA            complement(20..91)
                     /locus_tag="IWO35_002736"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="IWO35_002737"
     tRNA            complement(113..200)
                     /locus_tag="IWO35_002737"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="IWO35_002738"
     tRNA            complement(225..298)
                     /locus_tag="IWO35_002738"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="IWO35_002739"
     tRNA            complement(312..382)
                     /locus_tag="IWO35_002739"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="IWO35_002740"
     tRNA            complement(384..459)
                     /locus_tag="IWO35_002740"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(478..551)
                     /locus_tag="IWO35_002741"
     tRNA            complement(478..551)
                     /locus_tag="IWO35_002741"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Met,seq:cat)
     gene            complement(564..653)
                     /locus_tag="IWO35_002742"
     tRNA            complement(564..653)
                     /locus_tag="IWO35_002742"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Ser,seq:tga)
     gene            complement(677..750)
                     /locus_tag="IWO35_002743"
     tRNA            complement(677..750)
                     /locus_tag="IWO35_002743"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Ile,seq:cat)
     gene            complement(764..837)
                     /locus_tag="IWO35_002744"
     tRNA            complement(764..837)
                     /locus_tag="IWO35_002744"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Met,seq:cat)
     gene            complement(865..937)
                     /locus_tag="IWO35_002745"
     tRNA            complement(865..937)
                     /locus_tag="IWO35_002745"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Ala,seq:tgc)
     gene            complement(957..1030)
                     /locus_tag="IWO35_002746"
     tRNA            complement(957..1030)
                     /locus_tag="IWO35_002746"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(994..996),aa:Pro,seq:tgg)
     gene            complement(1040..1113)
                     /locus_tag="IWO35_002747"
     tRNA            complement(1040..1113)
                     /locus_tag="IWO35_002747"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1077..1079),aa:Arg,seq:acg)
     gene            complement(1124..1209)
                     /locus_tag="IWO35_002748"
     tRNA            complement(1124..1209)
                     /locus_tag="IWO35_002748"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1173..1175),aa:Leu,seq:taa)
     gene            complement(1226..1297)
                     /locus_tag="IWO35_002749"
     tRNA            complement(1226..1297)
                     /locus_tag="IWO35_002749"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1263..1265),aa:Gly,seq:gcc)
     gene            complement(1309..1381)
                     /locus_tag="IWO35_002750"
     tRNA            complement(1309..1381)
                     /locus_tag="IWO35_002750"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1346..1348),aa:Thr,seq:tgt)
     gene            complement(1399..1480)
                     /locus_tag="IWO35_002751"
     tRNA            complement(1399..1480)
                     /locus_tag="IWO35_002751"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1444..1446),aa:Leu,seq:tag)
     gene            complement(1488..1560)
                     /locus_tag="IWO35_002752"
     tRNA            complement(1488..1560)
                     /locus_tag="IWO35_002752"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1525..1527),aa:Lys,seq:ttt)
     gene            complement(1564..1636)
                     /locus_tag="IWO35_002753"
     tRNA            complement(1564..1636)
                     /locus_tag="IWO35_002753"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1601..1603),aa:Val,seq:tac)
BASE COUNT          438 a          460 c          391 g          358 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
      481 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc
      541 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
      601 ccgcctgacg gttttcaaga ccgttccctt cagccggact tgggtaatcc tccatatgaa
      661 acttacaacg ccacaatgga ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
      721 gtctgctcta accaactgag ctaaaggtcc aggctcttaa aaatcgcggc ggaggggatc
      781 gaacccccga cctcccgggt atgaaccgga cgctctagcc agctgagcta caccgcgaaa
      841 aagatattca ttatttaaat gaaatggagc ctagcgggat cgaaccgctg acctcctgcg
      901 tgcaaagcag gcgctctccc agctgagcta aggccccgtg tttaaattta taattatcgg
      961 gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta ccaagctgag
     1021 ctacttcccg tttaattaac gcgcccaaga ggagtcgaac ccctaacctt ttgatccgta
     1081 gtcaaacact ctatccagtt gagctatggg cgcatatttg aaatgccgag gaccggaatc
     1141 gaaccggtac ggtgatcact caccgcagga ttttaagtcc tgtgcgtctg ccagttccgc
     1201 caccccggcg cgattgcttt ggcaaagcgg aaaacggggt tcgaacccgc gacccccacc
     1261 ttggcaaggt ggtgctctac cactgagcta tttccgcgtg tttaaagatg ccggctaaag
     1321 gacttgaacc ctcgaccctc tgattacaaa tcagatgctc taccaactga gctaagccgg
     1381 catgtaaaac ttaaattatg cgggtgaagg gacttgaacc cccacgccgt aaggcgctag
     1441 atcctaaatc tagtgcgtct gccaattccg ccacacccgc aaaaatatga gccgtacagg
     1501 gctcgaacct gtgaccctct gattaaaagt cagatgctct accaactgag ctaacggctc
     1561 atatggaggt taacgggatc gaaccgctga ccctctgctt gtaaggcaga tgctctccca
     1621 gctgagctaa acctccatgg ataactt
//
LOCUS       DACECZ010000221         1632 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.231, whole genome shotgun sequence.
ACCESSION   DACECZ010000221 DACECZ010000000
VERSION     DACECZ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.231"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..289
                     /locus_tag="IWO35_002754"
                     /pseudo
     CDS             1..289
                     /locus_tag="IWO35_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            420..1370
                     /locus_tag="IWO35_002755"
     CDS             420..1370
                     /locus_tag="IWO35_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0317754.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
BASE COUNT          597 a          226 c          264 g          545 t
ORIGIN      
        1 gatgatattt ttttaatttt agcaatactt tttcttgttt catctaattt tagtgataac
       61 ttaattgaag ttcttacaga accagaaatt cggtctatga cggatgaaga acaacagatt
      121 attaaagcta tgatttcgga aaataagaaa gacgtagtag ttatcaaaaa gattagagag
      181 tatacaaatt taggtgtttt acaagccaaa aaagtatatg aaaaaaaaaa aaagggtcta
      241 taattacaaa ttagataata cattagaagc tggagacaag attgtctaga taatgtgtga
      301 gataaaattt ttggcaggtt atcatacata agacaaaatg tttttaaaac tgataacgta
      361 tttattccac aacaattacg catttatttc tgagaaaaat ataaagcagg aggattacaa
      421 tgctgaaaac ttattcctta acaaaagata cattattagt ttcgtcagaa tcaaatatca
      481 aaaattcttc tgttgtttat gtttatggtg acgaacgaga atatatcgaa tcttctgaac
      541 ataaatatga ctttaaaata ggcaatatac taacgtttga tgatcgagtt gcatatgaca
      601 cgatgataga taagaacgat gaaaaaagtt tgctcgtaag ttttttgttt cccgaggtac
      661 atgaaggtgg gcatctagat accctgacga catctgttgt tttcttggta tttaaaaata
      721 agttgataat ttttacaaaa caaaaattaa aattcattcc cgaactctta tccaatatgg
      781 ttttacgtga tgtcaatcat ttcgtggagg aagtactttt aaagatcatg caaaaagatt
      841 tctatatgat gcttaacgac cttcgtggag tcaaggaaaa aattgatgat ttagactcag
      901 aaatatcaac gtccggttca ctcaaaccaa cgtttggtga tttattgact cttcaaaaat
      961 acatgatagc tttgtcaaca acttataaag ctaaccataa agcgcttgat ttcattaaaa
     1021 aaaatttcac aactattgac gatattgatt ttagtactaa aaaaataatt gatgatttat
     1081 acgatacaag tgatacaatg gatagaatca ttttaagtta cagccaatat ttggacaaat
     1141 tggaaaatat gataaacaat atgatttcgt atcagctaaa catgattatg aaaaccctaa
     1201 ccgagatatc aattgttttg actattccag caattatttt tggcttttgg ggaatcaatg
     1261 tccatgtccc gtttgaaaag tcttcttatg gtgttttagc cgtattgatt atctctgcta
     1321 tactaagtct aatttgctgg cttttgatgc gacgcaaaac atatttgtaa agtatttgtg
     1381 ataccctaat atgatttaaa gcagaatttc tttgttgact ttttaaaagg ctgattcctt
     1441 tcaataattt aatagcttca gcggctgctc agaagtcaat aaattggact atttttattc
     1501 agttacttag gcaacatttg agatacgatt gcgattataa aaattgatat accaaccaat
     1561 ggctgcttgg aagactgaca tattagcctc gatatcatcg aataactaaa tacagaaaaa
     1621 tacaataaat at
//
LOCUS       DACECZ010000222         1573 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.232, whole genome shotgun sequence.
ACCESSION   DACECZ010000222 DACECZ010000000
VERSION     DACECZ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.232"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            63..1529
                     /locus_tag="IWO35_002756"
     CDS             63..1529
                     /locus_tag="IWO35_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ0317755.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          437 a          291 c          330 g          515 t
ORIGIN      
        1 ttaaattttt cggtaaagat gtataatatt ttctgaaaat ttaaagaatt ggggtataga
       61 ttatggaaaa acagcaagac aagggcttct tcgggcagcc gaagggattg tcaactttgt
      121 tcttcactga gatgtgggaa cgatttagtt attacggtat gcgtgcatta cttatctatt
      181 atatgtacga tacagttgcc aacgggggac ttggattacc acgagcaaca gcattagcta
      241 tcatgtcgat ctacggatct ttgatctaca tgtctagtat cgtcggcgga tgggtatctg
      301 accgagtgtt aggagcaaaa cgcactgtat tcttcggtgg agttttcatt atgcttggcc
      361 acatcgtctt ggctttacca tttggtgtaa gtacattgtt tgtttcgatg gcattgatta
      421 ttatcggaac cgcctttttg aaaccaaatg tttctggcat ggtcggacat ctttattcaa
      481 agactgactt gcgaagagat gcaggatttt cgatttttta catgggtgtc aacttagggg
      541 cattattagc accactaatc gttggtacaa tcggtcaaga atataactat catttagggt
      601 tctctttagc tgctattggg atgttctttg gattattaca atacatcatc caaggacgca
      661 agacattaga cggagtgggt acaacaccgc cgaatccttt gacaactgaa gaacgtaaag
      721 gattcatccg taacttgatc atcgcacttg ttgttatcgc tgcagtattc ggcggggcac
      781 aactaactgg tcatctgaca attgactttt ttgtcaatac aatcagtgta ttggggatta
      841 tcttaccgtt gtattacttt atcaaaatgc tgacttcaaa agatgtaact gctgaagaaa
      901 aaccaagagt atgggcttat atcccattat tccttgcagc aatcattttc tggtcattac
      961 aagaacaagg ttcatctatc ttagctttat ttgctagtga acgtacacaa tctacgctat
     1021 ttggtgtaac gatccaagca agctggtatc aatcattgaa tccaattttc atctttattc
     1081 tgacaccagt attcgtaacg atgtggacga aacttggtga acgtcaacca tctacagtag
     1141 tgaaattctc tcttggactt ttatttgcag gattatcgta cttgctattg atgttgccgg
     1201 gtatgcttta tggtacaagt ggaaaagtaa gtccattgtg gttgttggga agtttcttca
     1261 tcgtggttat tgctgaattg tgtctttcac cagttggttt gtctgtgaca acaaaattag
     1321 caccacgagc atttgaatca caaacaatcg ctatttggtt cttagctgat gcttcttcac
     1381 aagcaatcaa tgcacaaatt gcacgcttct acacaccggg aacagaatct gcttacttcg
     1441 gtattatcgg tgcagtagca atcgtcggtg gtatcttgct atttactgtg aaagaaccaa
     1501 tcaaaaaact aatgggctct attcattaat caatgaacat aaaaacagga attgcttatt
     1561 tgcagcagtt cct
//
LOCUS       DACECZ010000223         1558 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.233, whole genome shotgun sequence.
ACCESSION   DACECZ010000223 DACECZ010000000
VERSION     DACECZ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.233"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            133..507
                     /locus_tag="IWO35_002757"
     CDS             133..507
                     /locus_tag="IWO35_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0317756.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(621..1250)
                     /locus_tag="IWO35_002758"
     CDS             complement(621..1250)
                     /locus_tag="IWO35_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0317757.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1384..1456)
                     /locus_tag="IWO35_002759"
     tRNA            complement(1384..1456)
                     /locus_tag="IWO35_002759"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1421..1423),aa:Thr,seq:ggt)
BASE COUNT          531 a          232 c          272 g          523 t
ORIGIN      
        1 gtctacactc tgagatgtaa gaaatatcat aagatatttc ttacatcttt ttgttttgag
       61 caggacttta gataatttta ttaatatatg ctagcatgct tttgtaagaa aaagaaagag
      121 ggcgtattag ttatgtatct taaaattacg gaacaagcac aagaaagatt acaaaaatat
      181 attgatgaag gcgcaacagt tatattggat ttagatgatg gcgttgggga atattctaaa
      241 atgggtgttt gctctttaga tactagtttt cgtttacttc ttcttgataa agaacagaga
      301 aaaaatgatt ataaactcca attagatagt gatattgggg atgtttatat aaaagattat
      361 tcaaaaatgt acatggatga acatatgacc ctttctttag atccacgtct aggtgtattt
      421 actcttgaaa gcccaagtgg ttctttagac agtcatgttc aattagtaga tctacgacca
      481 gaaaaattct ctggaataaa acaataactt tctctctatt gtccataaaa ctagtctcaa
      541 tcaagctgta ttgaaaaaac aagacaacat tcttaccatg gtaaataaaa agacgacaca
      601 ttttgtcgtc tttttttaaa ttaactattt tcgataaaag atcgcatagc ttgtgccatt
      661 atttctggct tttctctatt caattcatgt tttccgtttt taataaagag tagccggcca
      721 ttcgttgaaa gtttatataa acgttttgct gctggtttat tcgcatgatc tttggtaccg
      781 cataaaatca atttaggaag tcgtgatgta cgaagctgtt ttttgcagtc taatgtttca
      841 agtgaacgaa gtagggtaag ggtttgtttt ttagatagac ctaatttttc aaatatctta
      901 gaaggtacgc agtaaaaata aatattctgg aaagtaagca ataagcgatt aggagactca
      961 aattgtggag cgactaaaac aacctttttt aaatatggag aaggagttgc aagatagttt
     1021 aaagcaataa cagcgcctaa agataatcca cataaaataa aaggttcctt tatctgattc
     1081 aattgtactt cgatgtattt ggttaagtcg tctaaagtag cagtgtctgt gctgtttaaa
     1141 tgattgaaca atgaaagact tttgcaagaa taactattgg gtagataaga gattacatct
     1201 ttccatgatt gttcgtcttg gcctagacca tgcaaaaaaa caattttcat attgctctcc
     1261 tttttatagt tatcatatag actttctttg tactattaaa atataaacat tcatttctta
     1321 cgtcataaaa taaaaaaaca gcgcagacac attgttaaac caacatgttg cactgtttaa
     1381 ggaagccact tgccgggctt gaaccggcga cctcttcctt accatggaag cgctctacca
     1441 gctgagctaa agcggcatta tcattaatac aaataatagg ataacgaata ttaatgtatt
     1501 tgtcaacaga attttttcga aaaaatgtgt ttctctaata ggaaaacaga ttttagaa
//
LOCUS       DACECZ010000224         1510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.234, whole genome shotgun sequence.
ACCESSION   DACECZ010000224 DACECZ010000000
VERSION     DACECZ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.234"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            73..483
                     /locus_tag="IWO35_002760"
     CDS             73..483
                     /locus_tag="IWO35_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0317758.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            497..853
                     /locus_tag="IWO35_002761"
     CDS             497..853
                     /locus_tag="IWO35_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ0317759.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          560 a          220 c          278 g          452 t
ORIGIN      
        1 ttattagcta ataattggta ttttaaaaga aattactgta gaattggatt ataattaaaa
       61 aggagtgaga aaatgataaa agtatatact tcgcctagct gtacttcctg tagaaaagca
      121 agagcatggt tgcaagcgaa tcaacttgaa tttgaagaga agaatatttt tgccgaaccg
      181 ttaacagaaa gtgagatcaa aaagatcttg gcagcgactg aaggaggagt tcaagatatt
      241 atttccactc ggtcgaaaat ttacgaaaag ctgggcatag attttaacga attaacttta
      301 aagcaaatga tcgtcttatt caaagaatat ccttcattat tgagacgacc tattttgatt
      361 gatgaaaaaa gaatactaat cggatttaat gaagatgaga tccggtcatt tgtccctcgt
      421 gaaattcgat gtgttgccag aaatctgatc tatttacaaa acaataacat cttaaattca
      481 tagggaggga gccttcgtgg aagtgaaagt ctatggatca gcgcactgtg taacaagtct
      541 gaaggcgaaa caatggttag aacaccatca attgccttat caatttgttg acttagaaga
      601 acgcgatcta agtacagaag aagcagaaga attatgcagt tttgaagaag tgcaagcaga
      661 acagctattt gcgacttggt ctgaggcttt tcacaacatt gcgattgacc tttcctgtgt
      721 aggaaaaaaa caactgattt ttttatgcac acaacatacc aatctcctca gacgtccttt
      781 gattattatc gatgaagttc tatttattgg atacaatgag cagttgatgg aacagcttat
      841 cttaaataaa tagactttaa ctgttttaat caagtcactg agcatttcat acagttgtaa
      901 cagtcgaaca cttagcatca agcgtgttat gtgttctttt tttgacttta tttttctact
      961 atagagacat gctcaattta tgtggtcaaa attcaaatct aggtggggat atagagttaa
     1021 aaatatattg acatgtgtaa atttacatga tatattcatt tctgttgttt taagtagcaa
     1081 agcaataaaa tagtatttta aaaaacatat aaaatagttg ttgacatgtt agaaatgaca
     1141 tgataagata actgagttgt taaaaaagaa ccacttgtga agcttgattc tatcgaaaaa
     1201 aacttcaaaa aaagttcttg acaaataaca aatgataagt tatgataaat gagttgctgt
     1261 aagatttcga aaaacgaaat caaaaaaact tttaaaaaag agttgacaat tacttgtctc
     1321 tctgatatga tataaaagtt gctacggcag ccaaaaacga atcggggaac actcctgatg
     1381 aagtagacct ttgaaaactg aacaaagtaa gacaaaccaa atgtgtaggg cgtcttgatt
     1441 caattcaaga caacaaacat tttttaacaa gcaagcaata tgctagcaaa caaattgagc
     1501 ttaacaatcg
//
LOCUS       DACECZ010000225         1487 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.236, whole genome shotgun sequence.
ACCESSION   DACECZ010000225 DACECZ010000000
VERSION     DACECZ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.236"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            15..1184
                     /locus_tag="IWO35_002762"
     CDS             15..1184
                     /locus_tag="IWO35_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0317760.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          527 a          269 c          238 g          453 t
ORIGIN      
        1 ctaaataaac tattatgaaa aaaggaaaac atgctaaaaa aagaaaatat tcatttttaa
       61 tgatcgttgt cgtgttgttt agccttagtg cgttctctac aggaactatt cttggatggc
      121 aattttacaa taatgaacac tctcagatag ttgcccatgc tcaatcagct caaaaaaagt
      181 tgatactgac taccaatgaa caacagctcg ttgaaaaaat cgatcaacta ttaagaaaaa
      241 atgattatat tggctcaatt tacgtaaaga aaaataatcg catcatcatg gaaaaaggct
      301 atggctatgc gaataaactc acgcaacaac caaatgatcc ttctctttat tatcagattg
      361 gttcggttca aaaagcattg actactcttt tgattttaaa acaagtcgag cgtgggcacc
      421 tttctttaga tactaagctg gctcaattct atccacaaat tgctggcagc cagcaaatta
      481 ctatccgtga cttgctgtat atgcgctcag gcttgcaccg aactgcttct ccaaccatac
      541 cgatgactga tgaagaagtc gttcaatttg cgattcagca cttaaaattt acagattatc
      601 agacctctca ttatgattca ttgaacttta ccatattgac tggaattttg atcaaattaa
      661 caaatcaacc gtatgaaaaa ttattgaccg atgatatcat cgaaccttta ggattacagc
      721 atacaaaatt ttatgaccaa gtcaaaaatt catccaatca tgcactatcc tatcaaatgt
      781 ctaaagagga tgattacttt aaaccactga cagaatctga aacagagatc cataacgaat
      841 tagggactgg taatatcagt atgtccgtct atgatttaaa tacgctgttt acacaagttt
      901 tatctggaaa actagtttcc aaagacttac tcttctcatt atggcgtgaa aatgcgaatg
      961 gacatccata tagtggtggg gtatatagcg gagataatta tatcctgtcg caaggaaata
     1021 tcgactcttt tgatacatct gctatattta gcaaagattc acaggatgct atcatcatgg
     1081 aaagcaatat aaaagctgac aaaactatca aactgcctac tgctgatcta agaaaccaaa
     1141 tctatgacct gatggaagga actactaagt tggcaaatag ctgatatgat gaaaagatag
     1201 caaatactac ttctttgaat agcatttgct taaaaaaagt actcgactaa acgtaggaaa
     1261 gagtgtggga caaatctaaa aatcaatttt tgctacagac tttaattcca aataaacaga
     1321 acagaaagag gtgttcttct ttctgttctg tccaaattat tctaagattt ctctttacca
     1381 acattatttg acaaagggcc tattaaatct aaaatagtat ctttagattg ctaaaatatg
     1441 tgctagcaaa tcattcatgg cttgttctta tttttaattt tatgcaa
//
LOCUS       DACECZ010000226         1476 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.237, whole genome shotgun sequence.
ACCESSION   DACECZ010000226 DACECZ010000000
VERSION     DACECZ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.237"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            151..1446
                     /locus_tag="IWO35_002763"
     CDS             151..1446
                     /locus_tag="IWO35_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0317761.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          465 a          290 c          277 g          444 t
ORIGIN      
        1 cctcaacgac gtttagttta tgaaaacagg ctctatactt tttgatgttg gtgaacttca
       61 ccaacatctt ttttgtataa aaaaatatag aaaagacacc ctgaaatcct ttagaattaa
      121 tttggctacc aattcatagg aggattcaag atgtctcata ctcagcttat caaagatacc
      181 ctaaatattc tagacctaaa tatctatttc gaggaaaatt gtttaactaa agaaaaatac
      241 aaagggcaaa tttgcttgat ttacaaagga actcttctgt acaagccaga agagtgttcc
      301 cattgtcttt gtgtcgtccc ctctaggatc attcgttggg ggacgacaac cgttcggttg
      361 ttactgaatg atgtgtctga gtatcgcact tatctggagc tgaagaaaca acgatttaaa
      421 tgtaagtctt gccaaagaac attcgtagcg gatacctctg ttgctgaaaa acactgtttc
      481 attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac ttccatgacg
      541 gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat gaagcgattc
      601 taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt tgatgaattc
      661 aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg acaaagccat
      721 gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta tttttctcgg
      781 ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta tgcgccttat
      841 gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg tttccacatc
      901 gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac ggtacgactt
      961 aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta ttggaaactc
     1021 ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc cagttttaaa
     1081 gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc caacttacgc
     1141 caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga tcagtcacta
     1201 tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac aaccacttta
     1261 cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag ctattcaaac
     1321 ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc gtacggcttt
     1381 cgcaactttt acaatttcaa actacgaatt atgattcgtc atggcaaaac atttttaacc
     1441 aaataaacag aacagaaaga ggttttcttc tttctg
//
LOCUS       DACECZ010000227         1444 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.238, whole genome shotgun sequence.
ACCESSION   DACECZ010000227 DACECZ010000000
VERSION     DACECZ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.238"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            62..1438
                     /locus_tag="IWO35_002764"
     CDS             62..1438
                     /locus_tag="IWO35_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0317762.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKTSNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
BASE COUNT          464 a          174 c          277 g          529 t
ORIGIN      
        1 agagtatagt acgtactttc atcagaatga aaacgcttaa caacagaagg gagagaattg
       61 tatggaaaga aaatttggta taagagacaa aataggatat atgtttggag attttggaaa
      121 cgattttact ttcatttttg ctagttcttt tttaatgatc ttttatacaa aagtattggg
      181 gataagtgga gctgccgttg gaacattatt tttagtagca agatttatcg atgcagttac
      241 tgatgtaact atggggcgta ttgttgatag atcgaaagca actaagcagg gtaagtttaa
      301 accgtggatt ttaagaatga gtggtccagt cgctttagca agttttttaa tgtatcaaac
      361 ttctatgatt tcagcttcta tgagcgttaa ggtagtatac atgtatgtta cgtatatatt
      421 atgggggagt attttttaca catctataaa tattccttat ggatcaatgg catcagctat
      481 tacttctatt acagaagaaa gaacaatgct atcgacattc cgaaatgttg gagctacttt
      541 agcaagtttg gtcattggtg tattaacacc attatttatt tatacaagag atgattcagg
      601 cgctcaaata gttcgtggtg gatcaacttt tacaattgtt gcgggaatat tttcgatatg
      661 tgcacttatt tgttatttaa tatgttataa actaactact gaacgagtaa gaattgacca
      721 atctcaatac aaagtttcat tgttaaaatc tttaaaaggg ttagttaaaa atagggcatt
      781 actttctata attggtgcgg ctattttctt actccttgct caattattaa ttatgagtat
      841 gaataattat gtatttcctg attactataa tgatgcacag ggaatagttt taatgaattt
      901 tatcaatcct attttggttt taatagttgt ttcaccttta actttgtttt taagtaaaaa
      961 atatggaaaa aaagaactgg catcagtagg aatgttcttt tcagcaatag tatatttgat
     1021 cttatttttg ataaaaacta gtaatatgta tgtattctta attcttagtt cggttggcta
     1081 tatgggtttg ggtgtgttta atactgttat ttgggcaaat attactgatg ttattgatga
     1141 tcaagaagta aagtctgatc aaagagaaga tgggaccgta tatgcggtat attcttttgc
     1201 tagaaaaata ggtcaagctc tagctggagg cgtcggaggt tggactcttt ctattatagg
     1261 ctatgactct ttagcaaatg tacaaacggg agatgtttta gaaaagttat ataacgcttc
     1321 aacattaatt ccatcaattt gttttctgat tgtaggatta attttatttt tcctttatcc
     1381 attatctaaa gataaagtct tagaaaattg cgagctttta aaacaaaaac ataaatagga
     1441 gaga
//
LOCUS       DACECZ010000228         1421 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.239, whole genome shotgun sequence.
ACCESSION   DACECZ010000228 DACECZ010000000
VERSION     DACECZ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.239"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            60..1004
                     /locus_tag="IWO35_002765"
     CDS             60..1004
                     /locus_tag="IWO35_002765"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0317763.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT          509 a          211 c          282 g          419 t
ORIGIN      
        1 aaacaagact atacttttca tgaaagtaac aaatagttat gaaaaggagc tgtttattca
       61 tgaaaaaaac aagtcgtaaa gtagtaatcg tcggtacagg ttttgttgga acaagtatcg
      121 cttatgcaat gatcaaccaa ggaatctcta atgaattagt attgatcgat gtgaatcaag
      181 aaaaagcaga aggggaagca ttggaccttt tagatggtat ggcttggggc gatgaaaatg
      241 tggctgtatg gtcaggtggc tatgaagaat gtaaagatgc tgatatcgtt gttatcacag
      301 ctggtatcaa ccaaaaacct ggtcaatctc gtttagattt agttaaaaca aatgcgtcaa
      361 tcatgcgcca aatcgtaaaa gaaatcatgg gatcaggatt cgacggtatt atcgttgttg
      421 cttcaaaccc agtagatatt ttgacatata tcgcttggaa cgaatctggt ctgccaacat
      481 cacgtgttat cggtactgga acaacattgg atacaactcg tttccgtaaa gaaatcgcat
      541 tgaaattaaa agttgaccca cgcagcgtcc acggctatat cttaggtgaa catggtgatt
      601 cagaagttgc tgcatggtca catacaactg tcggtggtaa accagtgttt gaaattgtag
      661 aaaaagacca tcgtattgcc caagatgaat tagatgtgat tgctgataaa gtccgtaatg
      721 ctgcttatga gatcattgat cgtaaaaaag caacttacta tggtattggt atgagtactg
      781 cacgtatcgt aaaagctatc ttgaataacg aacaagctgt attgcctgta tctgcttatt
      841 taactggtga atacgacgaa aaagatatct ttacaggtgt gccatcaatc gttgacgaaa
      901 atggtgttcg tgaagttgtt gaattatcaa tcaacgaaga agaaaaagca atgttcagca
      961 aatcaacaag tgctttaaga gaagtattga acactgtatt ataagaaaca atgaatatga
     1021 actaaaaatc ctaaaactgc ttaacagctg aaatagctgt taagcagttt tttattgata
     1081 tctaataggt aagcaaataa acagaatggt taaaattaat cggtccgaaa atgtatccgt
     1141 acatatgaac atatgaaatt taatctgttg attacaataa gcgaatcgtt atcaatgttt
     1201 atctgtggtt aattaaaaaa tccttccgca acggcaaata caatgattca tatctaattc
     1261 gttcatattt gattaactga ataaagaaac tggtttttat cattcaatta aacgacaaaa
     1321 aaagtaagaa aaactgcaaa atttttttat aattttgcat aagcagattt tgatggaaaa
     1381 atgacgattt ttgataaaaa accgttcgag agaggtttat c
//
LOCUS       DACECZ010000229         1401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.240, whole genome shotgun sequence.
ACCESSION   DACECZ010000229 DACECZ010000000
VERSION     DACECZ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.240"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(65..>1401)
                     /locus_tag="IWO35_002766"
     CDS             complement(65..>1401)
                     /locus_tag="IWO35_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0317764.1"
                     /translation="IIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQ
                     RSKIARLLTEIVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGE
                     EINIRNVLFGVYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLT
                     TISLLRAYGNQPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLF
                     LITEEEFELDGAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRM
                     GMMHVNRKNWIFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFM
                     IRLFYDYLFLLVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRI
                     TSRTNIYLSDCLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          452 a          248 c          203 g          498 t
ORIGIN      
        1 ttttctttga aaaaaacata aatcagctaa taaatatgtg agacaaagct gccatccata
       61 aatactagct gattttttca gttaaatgtc tatttttttc tctgatgatg gtatttccaa
      121 aatattccca gtcatctgga gtcggtggat ttttccaaat tatctgttct ttattaaatt
      181 tttcaagtaa acaatcagat aaataaatat tcgtacgaga agtgattcga ctctcaatca
      241 caatattgta atttttaaat cctttcactt gagaaataat gtaatcggta taagcagatc
      301 cttgcgaaaa atcaatacag acatgaatcg gtgcttcaaa atatgatacc ggacaaatac
      361 cgaccaataa aaagagataa tcataaaata agcgaatcat gaaacgttca tcttttccca
      421 agaaatagcg tttattctct tgtatgacct tttggattgc tgtactgaat attggatacg
      481 tctcgaagaa aaactgtagc tgctttttag acgtaaagct agtcatctca aagtcgaata
      541 tccaattttt tcgattgaca tgcatcatac ccatacgaag ttcttttcgt atatatagtc
      601 tttcttcttc agagatttca atcgaaaatg gtaattgatc aatcaattcc actgacattt
      661 tttgactaat catgtggata tcatgaaaat gagcttccgc aagtccaaat gcgccatcta
      721 attcaaattc ttcttccgtg atcaaaaaca acatgatata agaaatctca tcaatgatct
      781 gctctttagg gatatgaaga aaagaaatat gtgataaatc cttgatgatc ttcattcctt
      841 ctgagctctt aaagtattca tctgaaaaga aacgttttcc gattggttga ttcccatagg
      901 ccctcaacaa actaatagtg gttagcaaat tgaatttgat tcgttttgtt gcagacagtt
      961 ttaaattgaa cagatattca aaatattgca cgatactctt tgcttcattc atgacgcttt
     1021 ctctaaatgg catagaaaaa ccattaaaga ccccgaaata taccccaaat aatacgttcc
     1081 gtatgtttat ttcttcccca actaatatgt ttttctttaa ctttatttga tgctgttcaa
     1141 gaaaagagac taattctctt ctttttacat aggctcttga tatgcttaaa aaatgattct
     1201 ctgcaaattt ttcgatcgtt acttttgaat ccacgatctc cgttaacagt cgagcaattt
     1261 ttgatcgttg aaagtagatt cttcttaaat ttttgatatc cgtcaaattc agacgttcga
     1321 catggatctc tgcttcttct gaaatttgga tctctggctt tgttttgaat tcagtaaatt
     1381 ctttatttaa ttctatgata t
//
LOCUS       DACECZ010000230         1374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.241, whole genome shotgun sequence.
ACCESSION   DACECZ010000230 DACECZ010000000
VERSION     DACECZ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.241"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            190..444
                     /locus_tag="IWO35_002767"
     CDS             190..444
                     /locus_tag="IWO35_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0317765.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            445..762
                     /locus_tag="IWO35_002768"
     CDS             445..762
                     /locus_tag="IWO35_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0317766.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          493 a          196 c          265 g          420 t
ORIGIN      
        1 atgttaacgg aagtgtatgg tgagtagttc actcgtattt tgatatcgtt tgtatatgtt
       61 ttcagcgttc taagtggctt attttgtcca ataataatat ggagtggaat cccttaagaa
      121 acaccgttgg caattgttgt tataacatgt tataatttat tcatcaaaaa ctaaaaggag
      181 tgtgataaca tgggaaaaga actaaaggtt agaaagatcg gcaatagcgt gggagtgatc
      241 cttccaagct ctcttggatt aaaaagcgga gacattatcc aagccaaaca agaagacaac
      301 ttattcatat tagatactac tcaaatagca aaagaacacg atcgcaaatt gattgaagaa
      361 agtttccaag actttgagaa aggtttaaca gtttcagaaa ttgaaatggt aaaagctttc
      421 ggaaaatacg gctggagtga atagatgaaa gagtacacgc tagtttattc taaaagtttt
      481 cagaccagcc ttgaaacgat aatagaagaa tgggaaaatg agcttgctct ttctccagcc
      541 agcataaaaa gatttgtagg tgcgatccag aaggctttag aactaaccaa aaattttcca
      601 actatgcatg aggaagtttc cgaaatctat ggaatggata tcccaacata tcggatcgtg
      661 atcggcaaaa gctatgcctt attttatcgg atcgaccatg aaaaaaaaga gatacttgta
      721 ggacgtattt tccaatctaa acaaatgaaa ctaggctttt aacaataatt aggaaaaagt
      781 cgctcatcct ctacaggtgg gtgattaatg ccgctcgata tggttgttaa ccgtcggtta
      841 ctcgaagatt gatatataag acttagtacc tttttatttt gctccatgtt atggccgaag
      901 gtctaaagat acaatcatta gtctagagaa aaattaagaa ccttaggaac tacggggatc
      961 gcttggtgaa taagagacac tgctattagt gaaaaaataa gatatcaagt acgctctatt
     1021 cccaaaaatt tcgtgacttt actcatgaga ggttcaagta gtatattttt aatcaaaatt
     1081 gacgtaactt ttaataaata ctacctaaat aaggtagtat ttatttttgt gttttaaaag
     1141 aggaagtcca atatatctat gggaaacttc ttaaatatta tgttggtaca tgaaaatata
     1201 cttccctgct agactattta caacatttta ctttgtacgt attcttgttt taaaaggaat
     1261 gggataaaat ataggtacaa taactatttg atgctaaata taattaatgg aggagagata
     1321 gtgaacatac ttaatgaaaa aagatatgta tggtttgctg gtaaaaaata tatt
//
LOCUS       DACECZ010000231         1370 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.242, whole genome shotgun sequence.
ACCESSION   DACECZ010000231 DACECZ010000000
VERSION     DACECZ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.242"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            35..1336
                     /locus_tag="IWO35_002769"
     CDS             35..1336
                     /locus_tag="IWO35_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0317767.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          446 a          275 c          253 g          396 t
ORIGIN      
        1 gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc atatcaaact
       61 actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga agagcattaa
      121 gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc gctgtccttt
      181 gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa aatcgctcat
      241 tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac aacgctacca
      301 ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata gaaactgctt
      361 tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac aagcaatgac
      421 gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac tcgcttctct
      481 gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa tggatgaatt
      541 tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg gcgatactgg
      601 aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt attttatgca
      661 atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga atgtttctta
      721 tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc gttttcacat
      781 tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga aaagctttga
      841 tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt ctcggcttct
      901 gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca attttggttg
      961 ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg acgaattacg
     1021 aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag aggcggacac
     1081 tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc attctataaa
     1141 aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc cttattcaaa
     1201 tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg cttatggctt
     1261 tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga ttacctatga
     1321 atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DACECZ010000232         1369 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.243, whole genome shotgun sequence.
ACCESSION   DACECZ010000232 DACECZ010000000
VERSION     DACECZ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.243"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            289..696
                     /locus_tag="IWO35_002770"
     CDS             289..696
                     /locus_tag="IWO35_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317768.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            710..1111
                     /locus_tag="IWO35_002771"
     CDS             710..1111
                     /locus_tag="IWO35_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317769.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1113..>1369
                     /locus_tag="IWO35_002772"
     CDS             1113..>1369
                     /locus_tag="IWO35_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317770.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSG"
BASE COUNT          515 a          166 c          238 g          450 t
ORIGIN      
        1 tttgagggga agtacatata gttgttgata tagttgctaa tattgttagc taatttgtta
       61 gtaaaactgt atacgaattt agatagtaaa aaaacagttt gttccttata tagaggcact
      121 tttactactg attaaatgtc agatatgctt acaaatatac ttataaatat gcttacaaaa
      181 ttagctaata tttccgttga caaagctttg ttactattgt taaactaaat tgactagcaa
      241 aagtgaaaca taaatttatt aaataaatgt aaaaattagg agaaatcaat gtcttataga
      301 gtacaattaa taatttcaga agatgtagaa agtcagcaat ttggcactaa tgttattaat
      361 aaagtaatta atccagctct ttctattaat gcgcctttaa ttcctactgc tttatcattt
      421 tctgtaacag ctgtggtgtc tgagatagag gatacagaaa agataaaaat agtagaaatt
      481 gaagttttaa acaagaatga aaaacaaatt ttttctacgg gtgaagtatc ggtaagcttg
      541 cctcctcaag ttaacgatat aaactttaat ataaacgcca gaaatgtctt ggttgaagaa
      601 gcaggagagc attatgctgt tgttaaattt aatggaactg agattggaag gcaaatcttt
      661 gatatcaagg tcaacaaacc agtggaaaaa aattaaggag acgatacgga tgcttgatat
      721 agtaccatca aatacacatc ggagtggtag cttaataaat tttgctgcga tttttgcttg
      781 cgcatcggta attctagcat ctccagttgt tgtggatgca agttctactc cgaaaactaa
      841 aaatgataat caacttgtaa ttactacaaa aaatgagatt aacacaattt caagtaatga
      901 tggcaaaatt tttgatgtga taaatacggt tttaaaaaga aaagctgaaa tcaatataaa
      961 atatgatgaa gatttaaact tgtatttctt tgttatcaaa acaacatctg aattgttttc
     1021 ctcagattac gatgttttgg atactctcga taatgtatta tctgactata aatatatggg
     1081 gaaatctgta gtagcaactt tgggggaata aaatgtttga ctttaatgaa tatttatcta
     1141 tttccaaaca aattgaatca gatgataaat ataattctaa agaatcttat agaagaactg
     1201 ctgtttcaag agcatactac agtgctttta aaaaatctga tgagtatttg aaagaaaatt
     1261 atgatattat ttataatgga agtagcggaa aaggaagcca ccaaaccgtt tggaatttgt
     1321 tttcaacagt taaggaattg aataccttag gaatacataa tagtgggta
//
LOCUS       DACECZ010000233         1343 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.245, whole genome shotgun sequence.
ACCESSION   DACECZ010000233 DACECZ010000000
VERSION     DACECZ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.245"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1342)
                     /locus_tag="IWO35_002773"
     rRNA            complement(<1..1342)
                     /locus_tag="IWO35_002773"
                     /product="23S ribosomal RNA"
BASE COUNT          278 a          403 c          265 g          397 t
ORIGIN      
        1 atcgtctgca ttccttagcc tcctgcgtcc ctccattgct caaacaaaaa caacgggtac
       61 aggaatatca acctgttatc catcgcctac gcctgtcggc ctcggcttag gtcccgacta
      121 accctgggcg gacgagcctt ccccaggaaa ccttagtcat tcggtggaca ggattctcac
      181 ctgtctttcg ctactcatac cggcattctc acttctaagc gctccagcag tcctcacgat
      241 ctgccttcaa cgcccttaga acgctctcct accaatacac ctaaaggtgt actccacagc
      301 ttcggtaata tgtttagccc cggtacattt tcggcgcagg gtcactcgac tagtgagcta
      361 ttacgcactc tttaaatggt ggctgcttct aagccaacat cctagttgtc tgtgcaaccc
      421 cacatccttt tccacttaac atatattttg ggaccttagc tggtggtctg ggctgtttcc
      481 ctttcgacta tggatcttat cactcacagt ctgactcccg gatatgaatg aatggcattc
      541 ggagtttatc tgaattcggt aacccgagat gggcccctag tccaaacagt gctctacctc
      601 catcattctc aattccgagg ctagccctaa agctatttcg gagagaacca gctatctcca
      661 agttcgtttg gaatttctcc gctacccaca cctcatcccc gcacttttca acgtacgtgg
      721 gttcggtcct ccagtgcgtt ttaccgcacc ttcaacctgg acatgggtag atcacatggt
      781 ttcgggtcta cgactacata ctcaaacgcc ctattcagac tcgctttcgc tgcggctccg
      841 tctcttcaac ttaacctcgc atgcaatcgt aactcgccgg ttcattctac aaaaggcacg
      901 ccatcaccca ttaacgggct ttgacttgtt gtaggcacac ggtttcaggt tctatttcac
      961 tccccttccg gggtgctttt cacctttccc tcacggtact ggttcactat cggtcactag
     1021 ggagtattta gccttgggag atggtcctcc cggattccga cggaatttct cgtgttccgc
     1081 cgtactcagg atcctcctag gtgtcttcca catttcgtct acggggtttt taccctcttt
     1141 gactgacttt tccaagtcat tcgactatct gaaagaacta ccatattgga gtcctacaac
     1201 cccaacaagc aagcttgctg gtttgggctt ttcccgtttc gctcgccgct actcagggaa
     1261 tcgaattttc tttctcttcc tgcaggtact tagatgtttc agttctctgc gtctacctcg
     1321 aatgtgctat gtattcacac aat
//
LOCUS       DACECZ010000234         1324 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.246, whole genome shotgun sequence.
ACCESSION   DACECZ010000234 DACECZ010000000
VERSION     DACECZ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.246"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(41..448)
                     /locus_tag="IWO35_002774"
     CDS             complement(41..448)
                     /locus_tag="IWO35_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317771.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(467..1285)
                     /locus_tag="IWO35_002775"
     CDS             complement(467..1285)
                     /locus_tag="IWO35_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0317772.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
BASE COUNT          381 a          224 c          187 g          532 t
ORIGIN      
        1 agtataactt atactttaat tgaataccgc atgaaaaaaa ttattttggt aatttttcaa
       61 actcttcttg tatttcttct cttatgtgtt cttcttttgc tactcctagt ctatgctgga
      121 tatatgagtc tgctaatgta ttaacaattt tttcgaaaga aattctccct ttttcacttt
      181 gtaactccgt catatagtct tgtaaaattg agagtttgct atttaatgcc ggagagatct
      241 ttttaggaac tgataccttg tttcttttat caggaattga aactggtcgg cctctttttt
      301 tcaatgtaat atttcttgtg ttactcaatg cactattgct aaagttatat tcaccgctta
      361 ttttcacatc ttggcgtttg ttattgggta ataatggctc aaattcacca ttctccatca
      421 ttttaggaat atcaaaatct ttaggcatta cttatgcaac tccttattat ttatttaaaa
      481 actcttgtct agcatctagt tctgccaaaa tatttataaa tacttggtgt gctttataat
      541 cccattgatc aaaatttcca tttttgtatt ttcttaaatg tataccaatt ttagaatacc
      601 ttctcaatct tttttgataa tgaatgagat ttttggatac cacggttccg tatttttcaa
      661 ttgcttcttc taggatttct tcatcaaaat catcatttgg ttctaccata caaggtacaa
      721 tatctataat ttgcaaatca agatcgtatc tatcgaccat gaatttttga taatttacat
      781 atttgttgac tccctctaag gattcttcag ctgtttgaaa tgaaattatc gaatagtctg
      841 aagctgccat agcattatcg gaaaattcgc taatggttgg tggtacatca ataaatattt
      901 cttgataatt gttctttatt ggaagaagta attttttcaa gacagtaact tggtctaatt
      961 cattttcatg tttattagca aaagcggaga atgatcgaaa actagtgtca catgccagaa
     1021 ggtcaagatt ttcatttacc ttaataatac tacttgctaa ttctccattt gcgacaccat
     1081 ttgttatagt ggttttgata tcggttatac cgaaagttct tgctatgact tgtgttaagt
     1141 tcgcttgagg gtctaaatca ataataagcg tttttctatt tcttatcatt gcattgtcat
     1201 aagcaagcat gctgactatc ttagttttac ctactcctcc tttaaaattg ccaactgtaa
     1261 atgtagtccc ttgaaaagta tccatgtaaa tcctcccaat ctttaattga taattaaagt
     1321 ataa
//
LOCUS       DACECZ010000235         1318 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.247, whole genome shotgun sequence.
ACCESSION   DACECZ010000235 DACECZ010000000
VERSION     DACECZ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.247"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            54..>1318
                     /locus_tag="IWO35_002776"
     CDS             54..>1318
                     /locus_tag="IWO35_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ0317773.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRI"
BASE COUNT          447 a          257 c          253 g          361 t
ORIGIN      
        1 tctagtatga ttaaatcacc acaaaataaa caactgagag gacgatacgc tatatgaatg
       61 attctatcaa aaaaatgctg agaataacag agaaagattt gatgattaca gaggtctctt
      121 acgagaccct tcagaagaaa aagacgttgg tcgtcgatgc tgttctctcg cctactcctc
      181 gtgcttgtag aagttgtggt tctactgtgg tagatggaaa cgggaaagca attatagtga
      241 aaaatggaaa aaaggaaacg attgtccgtt ttgaacaata caatcatatg cctttggtta
      301 tgcgcctaaa aaagcaacgc tatacctgta aaaactgtcg cacccattgg acggctcaaa
      361 gttattttgt ccaacccaga cattcaatcg caaatcatgt tagatataaa attgcttctt
      421 tactgactga aaaagtatct ttatctttta ttgcgaaaag ctgtcaggta tctttgacca
      481 ccgttattcg tacattgaaa gagtttaaaa gctatttacc aaagcaatct aagaagatcc
      541 tccccagagt attgatggtt gacgaatttc gttcgcatgc ttccatagaa gataagatga
      601 gctttatttg cgcggatggc gaaacaggaa aattaataga tgttttgcct acgcgtaaat
      661 tacctcgatt aacaagctat ttcttaggtt gtaccaatcc agaagaagta gaattcttgg
      721 tgacagacat gaacgccgcc tacttccagc tcaccaaacg tgttctgcca aatgcgaaag
      781 tcgtgattga tcggtttcat attgtcaaac acatgaatca agcgttcaat gagttgcgta
      841 tccgtgaaat gaatgaactt cgtaaagccg gacagaaaag ccaggcagaa aaactgaaaa
      901 agaactggcg ctttttgcta aaaaatcgtg caaacatcaa ccattatgaa tacaaaacat
      961 ggaaaagttt ccgagcacca aaatacccat ttcttactga agcaatgatg attgatcgat
     1021 tgcttgagtt ttctccgccc ctaaaggagg cgtatccctt ttttcatgaa ttagttgaag
     1081 cctttcgaga caaagaccct gacttatttt tctccttatt ggcagaactt cccgaaacgt
     1141 tagatgacag ctttcgggaa aagcttcaaa accttctgac ctatgaagaa ggcatcacca
     1201 acgcaatgat ctatccttat tccaatggaa aaatagaagc gaagaatacc cacataaaga
     1261 caatgaaacg agtatcctac ggatttaaat catttgagaa catgagaatt agaatctt
//
LOCUS       DACECZ010000236         1311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.248, whole genome shotgun sequence.
ACCESSION   DACECZ010000236 DACECZ010000000
VERSION     DACECZ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.248"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            200..1248
                     /locus_tag="IWO35_002777"
                     /pseudo
     CDS             200..1248
                     /locus_tag="IWO35_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180785.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          532 a          148 c          254 g          377 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc t
//
LOCUS       DACECZ010000237         1280 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.249, whole genome shotgun sequence.
ACCESSION   DACECZ010000237 DACECZ010000000
VERSION     DACECZ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.249"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..723
                     /locus_tag="IWO35_002778"
     CDS             <1..723
                     /locus_tag="IWO35_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAQ0317774.1"
                     /translation="DFLSTPFDEESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGL
                     PVLLSTGMADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPV
                     GYSDHSLGYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTE
                     IFLGDGVKRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQ
                     YIGKKVKRPISADSVLLEEDVE"
     gene            720..>1280
                     /gene="neuC"
                     /locus_tag="IWO35_002779"
     CDS             720..>1280
                     /gene="neuC"
                     /locus_tag="IWO35_002779"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAQ0317775.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSF"
BASE COUNT          423 a          169 c          274 g          414 t
ORIGIN      
        1 gattttttgt ctactccttt tgatgaagaa agtgcagact ttctaaaaca aattggtgta
       61 caaggattta aaattggctc aggagattta acaaatttac cttttcttag aaaaatagat
      121 atgtatgggt tgccagtttt attatctaca ggtatggctg atattcatga ggtcagtgag
      181 gctgtagatt gtttcataga tagtccagtt actattttac attgtacttc aaattatccg
      241 gctagttctg aagatattaa tttattggcg attaacacat taaaagaaat gttcgggata
      301 cccgttgggt actccgatca ttcattggga tatgatgttg gtatttgtgc tgttgcgatg
      361 ggtgctaagg taattgagaa acattttacc ttggataagg aactacctgg tccagatcat
      421 aaagcttctt tagatcctca ggaatttgca gattttgtaa agcatattag aaacactgaa
      481 atatttttag gtgatggtgt taagcgagca atgccttctg aacttagtac aaaagaagta
      541 tcaagaaaaa gcctagtggt cactgagaat ttaaatgtgg gagatattct tacagagaaa
      601 tctcttacaa taaaacgtcc tggaaatgga atagctccta agtttttata tcaatatatt
      661 ggcaaaaaag ttaaaagacc aataagtgca gatagtgttc tgcttgagga agatgttgaa
      721 tgaaaaaaat acttatagta agtggaacac gagctgatta tggaatatat aaacctgttg
      781 ctgagaattt actacaagat aattgggatg tatcatttgt tgtttctgga atgcatttat
      841 cccataaata tggatttact aaagaattga tcttagctga cggatttaga attagtggag
      901 aagttcaaag tttgttttta gagaacaata agggaaacat ggctcggtct gtggcattag
      961 agataaatgg tttcacaaat atatttgaac aaactagccc tgattttgta ttattactag
     1021 gggatagagg agaaatgttg gcagctgcga tagcttgttt gtatcttgga atcaaaacag
     1081 cacatattca cggaggcgaa gtgtctggta ctgtagatga atcaataaga catgccataa
     1141 cgaaaatagc atctgttcat tttgctgcta cggaaaaaag caaagagcgt ttaataaaaa
     1201 tgggggaaga ttcttggaga gtttttcccg tcggagctcc tagaatagat actattttaa
     1261 ataatagatt acctagtttt
//
LOCUS       DACECZ010000238         1271 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.250, whole genome shotgun sequence.
ACCESSION   DACECZ010000238 DACECZ010000000
VERSION     DACECZ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.250"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            18..1025
                     /locus_tag="IWO35_002780"
     CDS             18..1025
                     /locus_tag="IWO35_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0317776.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1037..>1271
                     /locus_tag="IWO35_002781"
     CDS             1037..>1271
                     /locus_tag="IWO35_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAQ0317777.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELS"
BASE COUNT          503 a          164 c          241 g          363 t
ORIGIN      
        1 cataggagga gatataagtg tttaaaggaa aaacaatatt agtaactggc ggaactggtt
       61 ccattggaag tgaaattgtt cgtcagcttt taaaatatga accaaaagct atcagaattt
      121 atagtagagg tgaagaaaaa caattttata tgcaacaaga gctctcaaat cacactaatt
      181 tgagattttt aattggtgat atcagagact ctcaaaggat tgattatgca tgtcgtgatg
      241 tggatattat ttttcatgct gccgcaatga aacacgttcc agcaagtgaa tataatccta
      301 tggaagctgt aaaaacaaat gtgatgggaa ctcaaaatgt aatagatgca gcgatcaaaa
      361 ataatgtaaa acattttgta ggtatcagta cagataaagt agtgagtcct actaacacca
      421 tgggtgcgac aaagttattg agtgaaaaat taataatagc tgccaatcag tataaaggaa
      481 aaacagaaac aatattttct tgtgtaaggt ttggaaatgt catggggtca agtggatctg
      541 ttattcctct ttttagtaaa caaataaaaa acaataaaga tattactttg acagatgttg
      601 atatgacacg atttatgatg tcaataccgg atgctgcaag attagttctg tctgctgcta
      661 tgaattcaaa atttggagaa acatatgtct taaaaatgcc ggcaatcaat attttaactt
      721 tagctcaatc attaataaaa atatataatg atacacatga tgaaaaatat agaggtgaaa
      781 taaaagttgt aggtatcagg ccaggagaaa aattatatga agagctaatg actacagacg
      841 aaattgaacg ctcatatgaa aatgataaaa tgtatatcat tccaccacac tatcatgcta
      901 ctgacgaact gtacaaagat tatgaaaaaa ttaaaatcac tgcatactct tcagatgcaa
      961 cagaattaat gagtggacag gaatttatta gaatcgcaaa agagaataat gtcgtgactt
     1021 ggtaaggagt tttatgatga aatcagaagt atatgtaatt gcagaaattg gtgtaaacca
     1081 taatggaagt ctagatttag ctttagaaag tattgataaa gctaaagaat gtggagctaa
     1141 cgcggttaaa tttcaaacat ttaaaacgaa taagctagtt tccaaaaagg ctccaatggc
     1201 aaaatatcag aaggataatc ttaaagagga cagttctcaa tttgaaatgc taaaaaaatt
     1261 agaattatct c
//
LOCUS       DACECZ010000239         1257 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.251, whole genome shotgun sequence.
ACCESSION   DACECZ010000239 DACECZ010000000
VERSION     DACECZ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.251"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            84..1169
                     /gene="xerS"
                     /locus_tag="IWO35_002782"
     CDS             84..1169
                     /gene="xerS"
                     /locus_tag="IWO35_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ0317778.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          503 a          213 c          191 g          350 t
ORIGIN      
        1 accaattgac aaaacactct ataattggta tagtccaatt atagagatac tcctctcccc
       61 tacttatcga aaggatgaat aacatgaaaa atacagaaat atatcaaaaa atagatacaa
      121 tcatacttca tatgcccgat tatataaaag attttgttca agatcgtgag gataaagatc
      181 aatcacctcg aactacttta gaatatctaa aaaattataa gctcttttat gaatggcttc
      241 tgagtgagtc tatcgttccc gatacttcaa tcacctcgat aagacagtta actgcctatg
      301 atctaaactt gtacaaatct catttgaaac gtcgcacaaa ggaaaatgta aaaaaagaga
      361 caacaaaaac taagctcaaa gataataata acttaggtct atctacttct actatcaata
      421 gaaatatcac agcattaaag gttctattta aatatctttc gaaaagttcc aataatcctt
      481 taggaaaacc atacttagaa aataatccta tggatcaagt agcaactatc acagataaaa
      541 ctacgttagc tgcccgtgca aacgcaatcg agaaaaaatt atttctagac gaagatacac
      601 aaaattatct agattatatt gccaatgaat ataaaaaaac tttgagcaaa cgagctttga
      661 tctattatca acgagatgta gaaagagacc tggcgatcaa tgcattgatt ttaggatctg
      721 gattaagact ctccgaggtt gtaaatatta atttagagga cctatcttta gaaaagaata
      781 atgtggtagt aactagaaaa ggaaataaga gagacgcagt aaatatagca gcatttgcaa
      841 tggaatattt agctaattat ctagctattc ggaaagaacg atacaaagta acagaaaacg
      901 aaaaagcttt atttctagca atttatcaag gagaagccaa gcgtatatcc ggtattgcaa
      961 tcgaacggat ggtagcaaaa tattcaaaag gattcagagt ccaagtcagt ccacataaat
     1021 tacgtcatac tttggctact agactctatc aacagacaaa ttcattggta ttaactgccc
     1081 aacaattggg acatacaagt acaaatacaa caactttata tactcatata gacaatgcag
     1141 ctacaattga tgctttgaac gctttgtaaa tgtagtaaaa aatagtataa taatgaagcc
     1201 tgtctctaaa attagataaa agcccaactt taaaggcaag cttcattatc attttta
//
LOCUS       DACECZ010000240         1257 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.252, whole genome shotgun sequence.
ACCESSION   DACECZ010000240 DACECZ010000000
VERSION     DACECZ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.252"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            161..1201
                     /locus_tag="IWO35_002783"
     CDS             161..1201
                     /locus_tag="IWO35_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ0317779.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          507 a          178 c          208 g          364 t
ORIGIN      
        1 actttaaagg tagtacataa ttcattttat ttctgatttt aggtagtatt taattaaaaa
       61 acagttttaa tgcgtttgca ttttttattt atctttgcta ttttcgaaaa gagtaggtaa
      121 ttttacataa ataaaacaac gtgaaagtag aggatctaca atgactgatt ttaaattttt
      181 taaggcggat agagtttaca acgaattatt ttatcaattt ccaaaagtct ttattgtttc
      241 tgacgaatac aaaaaaatga aagattcaac taagattgcc tatatgcttt taaaagcaag
      301 attagagatc gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt attttactta
      361 tacgacaaat gaactatgta gagtattaaa ctgccaaaaa caaaaagcga tagcaatcaa
      421 aaaagagttg gaatcctttg gtttattatt acaaaagcag atgggattta acaaacagtt
      481 agggaaaaat aatcctaata gactatatct agcagaatta aaagtctcag aaaatgatat
      541 ctacttactc gaaaaatttg atagagagaa tagggaaaac gttgataaat cagagggtat
      601 gaaaatcata cccaccctcg acgaaaaatc agacgctgaa tcccttgggg ctcaagaggg
      661 tatgaaaatc ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa tcataccaga
      721 acttaataat aatatattag acactaatag acacaatata gacactgaaa aagaccgcct
      781 acaagatcaa ttgttgttag acaattttga gacaattatg acaaacgaca gcattgctac
      841 gtttgtccct gaacgagtat taaatttgat aaaaacattt tcttcaagtt acagtgaagc
      901 tcaaaaaacc gtccagacta ttcataatgc aaagaaaaaa gctgaaatag aaagtggtat
      961 ttcgatagtt tttgaagaac tcgatagtta ttatgtcaat gcagaacaag aattatacac
     1021 gacactgtta aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata tccagaacct
     1081 gatttttgtc tatgtaaaaa attggtttat cgaaaaacca atagctgcta aagtatcaag
     1141 tgaaaaacgt ttgaattatg aaagctcccc aagcactatt acgaaagact ggttagagtg
     1201 aaactttact gacaaagtga ttgttgcttc atggagtaac attaaaatta aaaattt
//
LOCUS       DACECZ010000241         1215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.253, whole genome shotgun sequence.
ACCESSION   DACECZ010000241 DACECZ010000000
VERSION     DACECZ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.253"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            414..947
                     /locus_tag="IWO35_002784"
     CDS             414..947
                     /locus_tag="IWO35_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ0317780.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DACECZ010000242         1209 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.254, whole genome shotgun sequence.
ACCESSION   DACECZ010000242 DACECZ010000000
VERSION     DACECZ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.254"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(195..>1209)
                     /locus_tag="IWO35_002785"
     CDS             complement(195..>1209)
                     /locus_tag="IWO35_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0317781.1"
                     /translation="GSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATLAGMFI
                     GVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKSDESQL
                     KVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIVLMNFI
                     NPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLVLTSVG
                     YMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVGGWTLS
                     IIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENYNLLNQ
                     KHN"
BASE COUNT          442 a          236 c          153 g          378 t
ORIGIN      
        1 aaaatctgtt tattccacga gtctctgaat ataaattaat attattccaa tcctgtagga
       61 atttttccct atgatgaaca tcaaaaatct ctgcagcaat tggcccgaaa aagagattgt
      121 gtttataagt cattgtatcc atgacacaac catctgaatc aatacaaaat atccaactgt
      181 tcatgttttt tctcctagtt atgtttttga tttagcaaat tataattttc taatactttt
      241 tctttagata atggatatac gaaaaataaa atcaatccta caacaaagaa acaaactgct
      301 ggaatcaacg ttgaagagtt atacagcttt tgtaaaactt ctccactttg tacttttgct
      361 aacgaatcat accctattat agaaagagtc caacctccga caccgccagc taaagcttgt
      421 ccaatttttc gggcaaaaga atatactgca taaatagttc catcttctct ttggccagat
      481 ttcacttctt gatcatcgat tacatcagta atattcgccc aaatgactgt attaaaaata
      541 cctaacccca tatatccaac agaagtaaga actaagaata catacatatt actaggcttt
      601 atcaaaaata aaagtaaata tacaatagca gagaagaaca tccctactga cgccaattct
      661 tttttaccgt attttttact gaaaattaaa cttacaggtg aaacaagtac caacatcaaa
      721 ataggattga taaagttcat taaaactatt ccctgtgcat tattataata atctggaaat
      781 acataattgt tcatactcat tgttaaaagc tgagcaagca acaaaaaaat agctgcagct
      841 attatagaaa gcaacgctct atttttagct aatcctttta aagatttcaa caatgaaact
      901 tttaactgcg attcatcact tttaacccgt tctacagtta atttataaca tattacgtaa
      961 caaataactg cacacactga aaatactcct gccacaattg taaaagttga tccaccacgt
     1021 acaatttgag ctccagaact atctcttgta taaataaaaa ttggtgttaa cacgccaatg
     1081 aacatacctg ctaaggtagc tcctatacta cgaaatgttg ataacgctgt cctttcttct
     1141 gcaacagaag tgatagctga tgccattgaa ccgtaaggaa tattaataga tgtataaaaa
     1201 atacttccc
//
LOCUS       DACECZ010000243         1198 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.255, whole genome shotgun sequence.
ACCESSION   DACECZ010000243 DACECZ010000000
VERSION     DACECZ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.255"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..283)
                     /locus_tag="IWO35_002786"
     CDS             complement(<1..283)
                     /locus_tag="IWO35_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0317782.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALAS"
     gene            complement(412..1059)
                     /locus_tag="IWO35_002787"
     CDS             complement(412..1059)
                     /locus_tag="IWO35_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ0317783.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
BASE COUNT          372 a          251 c          140 g          435 t
ORIGIN      
        1 aacttgctaa agcgactgga ccactcatcc gtaaaatcca cggtttaaat ttgccagttt
       61 tagatggttt tgatccatcc acaatacgcc ccattgttat gtctgtaact gcatcaataa
      121 atcttgctac taaaaataat gttcctacgg tagctccgct tatccccaat acttttgtat
      181 aaaaaatcat taaaaaagaa ctagcaaaaa tgaaagtgaa atcattccca aaatctccaa
      241 acatatatcc tattttgtct cttagaccaa attttctttc catataattt gcgctccttc
      301 ctattgttaa gcgttttcat tctgttgaac tgccaactta cgtactatac tctgaagtat
      361 aaagagttag tatttatttt ttacatttta tagtattatt tattttttct cctaataatt
      421 ctttatctca taaaacttat ctatatattt actagctatt cgagttactt cttcaaaatc
      481 acctttttca acaggagcca acaaattccc acctacacca actgcaacaa cgcctgcttc
      541 aaaccactct tctaagttat ctagacttac tccgccagta ggcattatat ttacatatgg
      601 taatggagct tgatacgttt ttacaacaga tggaccaaaa gcagaacctg gaaatagttt
      661 aatgatttct actccagcgg taagtgcctc ctttatctca gtaattgtca tacaacctgg
      721 taaataagga atttgatata aattacaaat ctctgctgtt tgtcgatcaa aagatggact
      781 tacgatatat tctgctccag ccataattgc aatacgagca gtaaacgcat ctaaaactgt
      841 accggctcca attattacat ctttttgatc agcatatttt ttagcaattt ctttgattat
      901 ttcttcagca ttcggaactg taaaagttag ttcaatccct ttaattccac ccgcagtaat
      961 tgcatttatt gctttaatgg cctcatcttt attttttcca cgtacaaccg caatgactcc
     1021 agccgcttct aacttactta aaatttctac ttttttcata tctatctcct tcttttatgg
     1081 atacatactc tagctagttg tactaactta caataaataa atttaagctt ttattttttg
     1141 tattattttc tactttcgat ttcaaaaaaa ttaaaataat tatacgcatt acgataac
//
LOCUS       DACECZ010000244         1194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.256, whole genome shotgun sequence.
ACCESSION   DACECZ010000244 DACECZ010000000
VERSION     DACECZ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.256"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IWO35_002788"
     CDS             <1..1076
                     /locus_tag="IWO35_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0317784.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tctt
//
LOCUS       DACECZ010000245         1179 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.257, whole genome shotgun sequence.
ACCESSION   DACECZ010000245 DACECZ010000000
VERSION     DACECZ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.257"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..763
                     /locus_tag="IWO35_002789"
     CDS             <1..763
                     /locus_tag="IWO35_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0317785.1"
                     /translation="EKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKIT
                     EEKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKE
                     LYANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLE
                     PMDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSL
                     DEIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            835..>1179
                     /locus_tag="IWO35_002790"
     CDS             835..>1179
                     /locus_tag="IWO35_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317786.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          521 a          134 c          194 g          330 t
ORIGIN      
        1 ggaaaaaatc ataaaaatat ttgataaaat taataattta ttcaagaatg acaatgctga
       61 attaaggaag ggtcttttag agtttaaact tcaagaacta gaacttcatt actcttattt
      121 agtgaaaatt accgaagaaa aagaacaaca aaaagcaata aaagaacaga tgatagaaga
      181 ggaaaaagtt cgtagagaaa ttgatcgtga aaagaaacgt attgataaag aacaacgtca
      241 attcaattct gaaatttcca aattaatcgc atatatgcaa aaagctaatg cagatgtgga
      301 aaaagaatta tatgcaaata aaattcaaga actggaagaa aaattaaaag aattagaagt
      361 tatacgtgaa aatgtattac aaagagaatt aaatactaga gccggttatg tgtatgtaat
      421 tagtaatatt gggtcttttg gtgaagacat atataaaatt ggtatgacta gaagattaga
      481 acctatggat agaataaaag aattgagtag tgcatcggtt ccatttgaat ttgacgttca
      541 tgcgatgatt ttttcagagg acgctccgtc tttggaaact aaattgcata atcattttag
      601 aaaacaagaa gttaataaaa ttaatcaaag aaaagaattt ttcaaagttt cactagacga
      661 aatagagaaa gttgttttag aaaattataa tggaacagta acatttacaa aattagctaa
      721 agctgaacaa tatagacgat ctttagaatt gtcaaaagat taaatatata aaaagccttc
      781 gggctttttc ttttaacaat taataggaac gtatattcga aaggaataga aataatgtgg
      841 ttagaaaaaa taaatgacaa aaaatttaag tacaccgaac gttatacgga tcccttaact
      901 gaaaaaaaga gaaaagtgtc cgttacttta tctagcaatt cacgccaagc ttggaatcaa
      961 gctaacttac ttttgaatga aaaaatcgca gaaaaaataa aacgaaatga agaattacca
     1021 ctaacttttg gagaattaaa aactaaatgg gatgaaaaat acaagccaac tgtaaaagaa
     1081 agttcctatc gaacaactca ggtttatcta tcattaattt caaaatatat aaaagatgat
     1141 gtacttgtaa aaaacgttaa tagtaatctt atacaagat
//
LOCUS       DACECZ010000246         1176 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.258, whole genome shotgun sequence.
ACCESSION   DACECZ010000246 DACECZ010000000
VERSION     DACECZ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.258"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            52..327
                     /locus_tag="IWO35_002791"
     CDS             52..327
                     /locus_tag="IWO35_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317787.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            754..>1176
                     /locus_tag="IWO35_002792"
     CDS             754..>1176
                     /locus_tag="IWO35_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0317788.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTD"
BASE COUNT          444 a          204 c          178 g          350 t
ORIGIN      
        1 accaaaggcg ctatgatttc catatgaaaa aaataaagtc aggtgaaaaa aatgaaactt
       61 gaggataaaa aagtcatttt gaagaaaatc aaacgactac cagaccaaat agatggctat
      121 acgatctgtt ctttcaaaaa agacaggagt ataaaagctt atacgcaaaa cacagaaaaa
      181 ggaataatct atttcctaca tgaagaaggt tacgataccc aaacctttat gtttactgaa
      241 aaaaaagaat ttcataaaca gatgaaaaaa ctgattgaaa aagaatttcc aagaagtcat
      301 aaactctatg tgaatcagca attttaactt ctaactgttc tttattaatt aaaaatgttc
      361 tgtaataaaa taaaacttta taacaactat aacaaaaccg aatgcaaaac agaagttcta
      421 taagttataa tcatagttgt taggtgattc atctagtaaa aacaccagat ttcaaatttc
      481 acacaattta aactagatca atcatcaacc ttcttcaaat tgttagaacc ctttcccgag
      541 gagctgcagc ataagcaaaa aaacaaacag gaagccaaat ggctaagaca ttttttctat
      601 tagtctagtt gtttctcgtc gcaagtttaa gactttagaa agttatttct aatgatgttt
      661 gggttgtttt gatcaatgct tatgcacagc tccttaccta ttatttatct caacagatct
      721 aacaaattaa tggactattt aaggagtttt tttatgctta cgatctattc ttctcgcaca
      781 catagttacc atctgatggc aaactggctg aaagaccatc aaatgcctta cagagaacga
      841 attttcacac ctaaacagcc tttaacaagg aaagagatat tttatttatt gtcattaaca
      901 gaaaacggat ttgacgatct attagctaaa cgctcgattg attataaaaa tttgaaagac
      961 caaatcgacc aatgtacgac ctcagagctt gtcgatctga tcgtctcgca ttttactttg
     1021 ttacaacgtc ccatcattac tgacggaaag aaattattag tcggattcaa tgaagatagc
     1081 atgcgtcttt ttatccctgt acaatggcgc agaagtcaac tgaaatctgt ctataacgat
     1141 tttacatggg taagcaatct agaaaattct acggat
//
LOCUS       DACECZ010000247         1167 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.259, whole genome shotgun sequence.
ACCESSION   DACECZ010000247 DACECZ010000000
VERSION     DACECZ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.259"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            24..284
                     /locus_tag="IWO35_002793"
     CDS             24..284
                     /locus_tag="IWO35_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317789.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(646..885)
                     /locus_tag="IWO35_002794"
     CDS             complement(646..885)
                     /locus_tag="IWO35_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317790.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          413 a          164 c          217 g          373 t
ORIGIN      
        1 aaaacattaa ggatggatga aatatgaata acgaaactat cattttagga aaaaaatata
       61 tttgccaacc tattggcttg aaacaaaaag tggttggaga agtaatcagt aaattagaaa
      121 attgtgttgt gatctgcgta gacgaatatc atgacgtcga tcgcaatgaa atattagaaa
      181 aatgcggaaa agtagtggca aaatatactc acgtttatga gttggaaaca acagaatatc
      241 tatataacta tagcaacaaa tatgaacata ctttagtttc ataaagattt aaataaagtg
      301 ccttcaaaag cgagaagacc aagcttttga aggcacttta tttagttggc agtgattgtt
      361 catgattttt gatttattca aaaggttcgc ctagagaatc tgtctctgta acgaggcctg
      421 gtggagaaac tcgataaaaa cctatactgc cagaactgtc ctattttcgg atagacaaac
      481 tagtagtttt aaaaagatca tccgatccaa tcatttcaaa aaatgcaaaa ctgatatcag
      541 gatcatttag ctcaggcttg ttatcgtaaa gcatttgtaa aggttgcatt tagttaggag
      601 taaacgcgaa actatcatca ttatttgctg aagaagatga agaagctaat tcagagtttg
      661 tttgaaataa aggagacgtg ctgacagttt ctttaccctg ttttttgcag aggtagaaac
      721 ttttatatcg gaagtttcta ccggagaatt tttctcattt gtagaaattg ttgaatcgat
      781 actattcgaa atttgcgtat ctgaaggtat gttctttgat gcagaagtcc cacaagcagt
      841 aagtaacaat aaatttgata aactgataaa cagaattctt ttcattatgt acacactcct
      901 tatagtatcg tttagcatac acctatgctc agtattaggc aaaatcaatg caaaactatc
      961 aagtctttta tagtatctat tagtcttagt aatacatata tggagagggt gatatcccag
     1021 ttcaaaccag atacattggt taccatctgt attaacgatt atgcctctaa acgctttttt
     1081 tatgaaaaaa tttataagtg aaaacgtagg tatgatatta tctagaggta atttttttgg
     1141 gtatttaaat agttgctagg aggatag
//
LOCUS       DACECZ010000248         1096 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.260, whole genome shotgun sequence.
ACCESSION   DACECZ010000248 DACECZ010000000
VERSION     DACECZ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.260"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..264)
                     /locus_tag="IWO35_002795"
     CDS             complement(<1..264)
                     /locus_tag="IWO35_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317791.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQV"
     gene            complement(308..538)
                     /locus_tag="IWO35_002796"
     CDS             complement(308..538)
                     /locus_tag="IWO35_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0317792.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            614..686
                     /locus_tag="IWO35_002797"
     tRNA            614..686
                     /locus_tag="IWO35_002797"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:647..649,aa:Lys,seq:ctt)
     gene            848..1069
                     /locus_tag="IWO35_002798"
     CDS             848..1069
                     /locus_tag="IWO35_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317793.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
BASE COUNT          357 a          175 c          135 g          429 t
ORIGIN      
        1 tacttgtttt tttgatactt ccaaataatt aagcaaatag tcataaaaca ataaatttgg
       61 attagcacat tcacattcca tagctaattg actttccaat tctagtaaaa attcatctct
      121 aattttttct gctcgttttt tatttccttt cttttttaat tttgtatctt tccacttttg
      181 tctaattttt ccgttagagt ctttataaga tacaactact tgtaatgttc ctcttttttc
      241 aattatgttt gctcttaatt tcaattcaat ctctcctctt aaactgaaat atcggcaaaa
      301 attttgacta ttttattata gcatgatttt taccttcatc ttgtattttc aatatatact
      361 caatgacagc ttttttaggt attttgtacc ttgctccaat ttttatattt tctattgatc
      421 tatctttcag caaattataa gctttatttt ttccaatatt taacatttta gttaaatcag
      481 taattttcaa tatcgatgga taactttcaa atattttttc ataatcttga aaatccataa
      541 aattcactct caattctgat aataagtatt gcactttttt attttaggtg ttagaataac
      601 aaagtattaa tttgggctgt tagctcagtt ggtagagcag atgactctta atcatcgggt
      661 cgcgggttcg agcccctcac agcccattgg gtgccaaacc cttaaataca gtgtaaacgt
      721 tggtgcttag catttagaaa acgtatatac tgtatttttt tgttttcaaa agagactgta
      781 gtaccccttt ttttcactaa aagcccactg tagtcattga tatttttgat aagaaaggaa
      841 cataattatg cgtaatgaaa ttgctaatat aacaataatc cgagaaactt tacaaaacca
      901 tactgcaaat gagatctcta aacatacagg attaaatttg agtactataa aaaaactaaa
      961 aagtggagaa cgttcaatag aaaaactaaa tttacatgat gctatttgcc tcactgaatt
     1021 tggtcttaaa aataatagaa aaaatgttga aatcaacata tggaaataat tcttaatcag
     1081 ctgcttttgt caaaag
//
LOCUS       DACECZ010000249         1081 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.261, whole genome shotgun sequence.
ACCESSION   DACECZ010000249 DACECZ010000000
VERSION     DACECZ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.261"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..970
                     /locus_tag="IWO35_002799"
     CDS             <1..970
                     /locus_tag="IWO35_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ0317794.1"
                     /translation="TSITHVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVNDLD
                     THYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPW
                     ILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHIYD
                     NPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSS
                     VSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCN
                     VDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          380 a          135 c          185 g          381 t
ORIGIN      
        1 tacgtctatt actcatgtaa caagtgatca ttattttgga aggaattttg attatgaaat
       61 atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt ttcgaaaggt
      121 aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag ctgactaccc
      181 tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa atttttctgg
      241 gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt ttgaatttat
      301 tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt taaaaaatat
      361 caatttagca aatataaatt atagtgatga acttccttta tcccctttac attggctatt
      421 agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc atatatatga
      481 taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat ttaatttaaa
      541 caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc aaataagttt
      601 gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat cctcagtatc
      661 tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt cagagtcaga
      721 aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag gtttgtgtga
      781 tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg ttgacaaagg
      841 aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata tgaataaaga
      901 agacttagat agtcataagt taattagtta tccaattata gaaaaacaac aaattaaata
      961 tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac aaaaaggcaa
     1021 agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt aatgttttta
     1081 a
//
LOCUS       DACECZ010000250         1058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.262, whole genome shotgun sequence.
ACCESSION   DACECZ010000250 DACECZ010000000
VERSION     DACECZ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.262"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(36..992)
                     /locus_tag="IWO35_002800"
     CDS             complement(36..992)
                     /locus_tag="IWO35_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317795.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgt
//
LOCUS       DACECZ010000251         1051 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.263, whole genome shotgun sequence.
ACCESSION   DACECZ010000251 DACECZ010000000
VERSION     DACECZ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.263"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            18..965
                     /locus_tag="IWO35_002801"
     CDS             18..965
                     /locus_tag="IWO35_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ0317796.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          337 a          163 c          229 g          322 t
ORIGIN      
        1 atatcggagg taaaaggatg agctgttatc ttatcaccgg tggggctggc ttcattggtt
       61 ctaccttagc caatcattta ggagaagaac atgtggtaat cgtcgtcgat gacttatcaa
      121 tgggaaaagt agaaaatctt gatatgacga aaaatatcac ttttattgag ggagatgtcg
      181 ctgagaaatc cttgatggaa gagatcatga aagctaataa atttgattat atctttcatt
      241 tagcagcagt tgctagtgtg gcagattcag ttgcccgacc agtagaaaca catcgtgtga
      301 attttgaaag tgtattaatg attttagaat tgatccgcaa gtatcaacct gatttaaaac
      361 ggatcgtgtt tagttcttca gcggctgttt atggggatga accgacttta ccaaaaaaag
      421 aagagtccgt gattcgccca ttgacgcctt atgcggttga taaattcgct gcagaacaat
      481 atgtgctaga ttattgtcat ttatatgatg tacccggtag cgcagttcgt tttttcaatg
      541 tctatggtcc aaatcaaaat ccgaattctc catacagtgg tgtgatttct attctcgtag
      601 atcgctataa gaaacaaatc gtaggagaag caacttcatt tacattattt ggtgatggaa
      661 gccagtctag agactttgtt tatatagatg atgtaatcca agcattactt ttagttgcga
      721 atgaggaagc tgcactgggg aaacaattca atgttggaac gggtaaagca accacacttt
      781 tggaattgat tcatgcaatc aatgaaattc tcggtgtaga attgacactt gaatacaaag
      841 aagagcgttc gggtgatatc catgactctc tagcagatat tactaagatc cagtcaattg
      901 gctatcaacc gaaatatggt attttaagtg gaatgactaa ttatttgaaa actgaaatca
      961 attaaaacat actaagataa gattagaggg taaattcagg ctctttgtca ataaggactg
     1021 atgagttttg taaaatcaaa tctgggatag a
//
LOCUS       DACECZ010000252         1044 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.264, whole genome shotgun sequence.
ACCESSION   DACECZ010000252 DACECZ010000000
VERSION     DACECZ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.264"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(62..280)
                     /locus_tag="IWO35_002802"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="IWO35_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="IWO35_002803"
     CDS             complement(546..740)
                     /locus_tag="IWO35_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317797.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          361 a          180 c          153 g          350 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caat
//
LOCUS       DACECZ010000253         1035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.265, whole genome shotgun sequence.
ACCESSION   DACECZ010000253 DACECZ010000000
VERSION     DACECZ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.265"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1035)
                     /locus_tag="IWO35_002804"
     rRNA            complement(<1..>1035)
                     /locus_tag="IWO35_002804"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACECZ010000254         1023 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.266, whole genome shotgun sequence.
ACCESSION   DACECZ010000254 DACECZ010000000
VERSION     DACECZ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.266"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(35..107)
                     /locus_tag="IWO35_002805"
     tRNA            complement(35..107)
                     /locus_tag="IWO35_002805"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(72..74),aa:His,seq:gtg)
     gene            complement(110..183)
                     /locus_tag="IWO35_002806"
     tRNA            complement(110..183)
                     /locus_tag="IWO35_002806"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(149..151),aa:Trp,seq:cca)
     gene            complement(190..270)
                     /locus_tag="IWO35_002807"
     tRNA            complement(190..270)
                     /locus_tag="IWO35_002807"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(234..236),aa:Tyr,seq:gta)
     gene            complement(272..347)
                     /locus_tag="IWO35_002808"
     tRNA            complement(272..347)
                     /locus_tag="IWO35_002808"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(312..314),aa:Phe,seq:gaa)
     gene            complement(383..455)
                     /locus_tag="IWO35_002809"
     tRNA            complement(383..455)
                     /locus_tag="IWO35_002809"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(420..422),aa:Asp,seq:gtc)
     gene            complement(465..537)
                     /locus_tag="IWO35_002810"
     tRNA            complement(465..537)
                     /locus_tag="IWO35_002810"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(502..504),aa:Val,seq:tac)
     gene            complement(553..624)
                     /locus_tag="IWO35_002811"
     tRNA            complement(553..624)
                     /locus_tag="IWO35_002811"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(589..591),aa:Glu,seq:ttc)
     gene            complement(639..728)
                     /locus_tag="IWO35_002812"
     tRNA            complement(639..728)
                     /locus_tag="IWO35_002812"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(692..694),aa:Ser,seq:gga)
     gene            complement(768..841)
                     /locus_tag="IWO35_002813"
     tRNA            complement(768..841)
                     /locus_tag="IWO35_002813"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(805..807),aa:Asn,seq:gtt)
     gene            complement(851..966)
                     /gene="rrf"
                     /locus_tag="IWO35_002814"
     rRNA            complement(851..966)
                     /gene="rrf"
                     /locus_tag="IWO35_002814"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          277 a          279 c          229 g          238 t
ORIGIN      
        1 ccttaccgct tggctatacc ccaagaataa aaagggcgaa tgatgggaat cgaacccacg
       61 aatgccggag ccacaatccg gtgcgttaac cacttcgcca cattcgccat ggcaggggca
      121 gcaggaattg aacccacact aacggttttg gagaccgctg ttctaccttt aaactatgcc
      181 cctataaaat ggaggagagt ggattcgaac cactgaaccc taaggaacgg atttacagtc
      241 cgtcgcgttt agccacttcg ctactcctcc atggtggcgc gggacagaat cgaactgccg
      301 acacacggag cttcaatccg ttgctctacc aactgagcta ccgcgccata ttttaaaatt
      361 attaattgaa taacaagaac gacggttccg acgggaatcg aacccgcgat ctcctgcgtg
      421 acaggcaggc atgttaaccc ctacaccacg gaaccagttc attatggagg ttaacgggat
      481 cgaaccgctg accctctgct tgtaaggcag atgctctccc agctgagcta aacctccaac
      541 attcaattaa aatgacccgt acgggattcg aacccgtgtt accgccgtga aagggcggtg
      601 tcttaaccgc ttgaccaacg ggccaggtaa atttgctacg gagagtaagg gattcgaacc
      661 cttgagacag tttggcacca tctacatgat ttccaatcat gctccttcgg cctctcggac
      721 aactctccag agtgaaagaa gctcaatact ctttctcaaa atgagaactc cggcagtagg
      781 actcgaacct acgacatcat gattaacagt catgcgctac taccaactga gctatgccgg
      841 aataacatca gcgtggcgac gtcctactct cacaaggggc aacccctcac tacaatcggc
      901 gctaagaagc ttaacttctg tgttcggcat ggttacaggt gtatccttct cgctatcgcc
      961 accacactgt ggtgttatct tttattgagt aaattttgtt cactcaaaac tggatttgaa
     1021 gtc
//
LOCUS       DACECZ010000255         1012 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.267, whole genome shotgun sequence.
ACCESSION   DACECZ010000255 DACECZ010000000
VERSION     DACECZ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.267"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(22..837)
                     /gene="thiD"
                     /locus_tag="IWO35_002815"
     CDS             complement(22..837)
                     /gene="thiD"
                     /locus_tag="IWO35_002815"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ0317798.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          264 a          222 c          159 g          367 t
ORIGIN      
        1 ctttttcaca acttctttct tttactctaa ttcaccagct gcccaatgat tgatggggcc
       61 atatttgtgc cctacattta tttcttcacg aattgcacgg tttacatagt cttttgccaa
      121 tcggatcgct tgttcaactg tttttccttt cgctaactca gctgcaatac atgcagatag
      181 cgaatcgcca gttccgttga tccggtcggt aggataaaat ggtgcattta accaaaatga
      241 ttttcctgat tcgaacaata cgtagtcatt gactgtttct tgctgcgcat ttttagcatg
      301 atgtcctttg atcataacgt tttttgcacc catatcttgc agttcttttg ctgcttgcac
      361 aaaatcttct tcagctgcta atgtccgtcc agtcagcttt tctgcttcat aaaaattagg
      421 tgtcagtact tcggccaaag ggacaagctt ttctttcaag ctttgcaaag cagattcttc
      481 tagtaaaaga tttccatgtt tcgttacgat tacgggatcc acgactaatg gtccaaaatc
      541 atatttttgg taatttttta caactgtctc aatcaacttt gaatctgcta gcatacctgt
      601 tttagctgca cgtatttgaa agtccttcgc aatcagttca aattctttgt cgataaaatc
      661 tgttggcaat gtcacacttg ctccgattcc gtaagaatta cctgcaacac atgcggtcat
      721 gacagaaaca ccgtaaacat ttcttgtaaa gaatgtatgc atatctgctt gcatccctgc
      781 gcttccatcg ctatctgatc ctgcgatcgt cattgcttgt acatactgat cttccatcaa
      841 atcgattcct ccatttttat aaaaagattt ttcataatac tgttactgtt tctatcatat
      901 gtttttcaaa gtatccctca gtaaatatac cactaccttt tcttggtaat caaatttatt
      961 tgaaaaaaca tttgcaataa atcagaaaat agtggtcgtt tatttgtatt cg
//
LOCUS       DACECZ010000256         1011 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.268, whole genome shotgun sequence.
ACCESSION   DACECZ010000256 DACECZ010000000
VERSION     DACECZ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.268"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            98..838
                     /locus_tag="IWO35_002816"
     CDS             98..838
                     /locus_tag="IWO35_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317799.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          373 a          180 c          195 g          263 t
ORIGIN      
        1 atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat ctgtgctatt
       61 attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca gacgaaaaca
      121 tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg acaagaagcg
      181 agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc cgtttgaaca
      241 gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca agcgtcgtgc
      301 aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata aaggaaaatt
      361 accggattat accggccatt accaatcaga aatcacatcg acaggacgaa aaaaactttt
      421 tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca agcatactac
      481 acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc ctaaatatgt
      541 acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc atttaatcga
      601 atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga cgccgtatca
      661 agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa cgttagagga
      721 aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag aacgctcatt
      781 acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt ttaaataagt
      841 gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt ttccttattt
      901 tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta agcgaatttt
      961 ttctcccaat aagtaagaaa ataatggcaa aataaaaaaa caaactattt t
//
LOCUS       DACECZ010000257         1009 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.269, whole genome shotgun sequence.
ACCESSION   DACECZ010000257 DACECZ010000000
VERSION     DACECZ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.269"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..848
                     /locus_tag="IWO35_002817"
     CDS             <1..848
                     /locus_tag="IWO35_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0317800.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          329 a          195 c          202 g          283 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaatttta
      961 tttatggtat gacataaata aagtaaagtt attcttctag gttaatctt
//
LOCUS       DACECZ010000258         1001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.270, whole genome shotgun sequence.
ACCESSION   DACECZ010000258 DACECZ010000000
VERSION     DACECZ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.270"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..623
                     /locus_tag="IWO35_002818"
     CDS             <1..623
                     /locus_tag="IWO35_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAQ0317801.1"
                     /translation="HIAIGITKDGDREIIGFMIQSGESEETWTTFFEYLKERGLQGTE
                     LVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTD
                     INLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLN
                     QEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            775..>1001
                     /locus_tag="IWO35_002819"
     CDS             775..>1001
                     /locus_tag="IWO35_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317802.1"
                     /translation="MISDNKWEINLKRFISEYIYTIETKSSFSLNEDSDPLKKYTIQC
                     KKNKDEKLFVIRPDLMKSNTHYIKEKPKDCD"
BASE COUNT          388 a          160 c          171 g          282 t
ORIGIN      
        1 gtcatatagc gattggaata accaaagatg gcgaccgtga aattatcggc ttcatgattc
       61 aaagtggcga aagcgaagag acctggacaa cattttttga atacctaaaa gaacgcggtt
      121 tacaaggtac ggaactcgtt atttctgatg cgcacaaagg attagtctct gccattagaa
      181 aatccttcac caacgtaagt tggcaaagat gccaagttca cttcctaaga aatatcttta
      241 ccaccattcc taaaaaaaat tcaaaatctt tcagagaagc tgttaaagga atttttaagt
      301 tcacagatat taacttagcg cgtgaggcta aaaatcgatt gattcatgat tatatcgatc
      361 aaccaaaata ttcaaaagct tgcgcatcat tggatgatgg attcgaagac gcctttcaat
      421 ataccgtaca aggaaattcc cacaatcgac taaagagtac caatctaatt gaacgactga
      481 atcaagaagt acgcagaaga gaaaagatta ttcgcatctt ccccaatcaa acatcagcca
      541 atcgcttaat tggagccgtt cttatggacc tacatgatga atggatttat tcttcaagaa
      601 aatacatcaa ttttgataag tagaaatggt aaaaacattg tatagcattt tacacaggag
      661 tctggacttg actaaaattt ctaacatcag ttattatgat aattttgatg atgtcaatta
      721 tgatttacag gaaagatata atgatttaat cgaagaatta gaggagagag gagaatgatt
      781 tcagataata aatgggaaat aaatctcaaa agatttattt cagaatatat atatacgatt
      841 gaaactaaat ctagtttttc tcttaatgaa gacagtgatc ctctaaaaaa atacactatt
      901 caatgtaaga aaaataaaga tgaaaaattg ttcgtaataa gaccagatct tatgaagagt
      961 aatacccact atataaaaga aaaacctaaa gattgtgact a
//
LOCUS       DACECZ010000259          982 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.271, whole genome shotgun sequence.
ACCESSION   DACECZ010000259 DACECZ010000000
VERSION     DACECZ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.271"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..977
                     /gene="uidA"
                     /locus_tag="IWO35_002820"
     CDS             <1..977
                     /gene="uidA"
                     /locus_tag="IWO35_002820"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAQ0317803.1"
                     /translation="VQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNL
                     MKWIGANSFRTSHYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNT
                     WKEIRTENSHKTVIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELD
                     PQKRPVTIVTYLKSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWM
                     ERCPDTPIMFTEYGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWN
                     FADFATSQGILRVQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
BASE COUNT          352 a          123 c          210 g          297 t
ORIGIN      
        1 gtgttcaggt taccaatggc aaatttttga ttaataataa acctttttat tttaaaggat
       61 ttggtaagca tgaggattcg tatgttaatg gacgtggttt gaatgaggct gtaaatatta
      121 aagattttaa tttaatgaaa tggataggtg ctaattcttt tagaacatca cattatcctt
      181 actcagaaga aattatgcga ttagcagatc gagaaggtat tgtagttatt gatgaagtac
      241 ctgcagtagg gttacattta aatttcatgg ccactttatt agatgataat gtggaaaaac
      301 ataatacgtg gaaagagata cgaacagaaa attctcataa aacggtgatc aaggaattga
      361 tagaacgcga caagaaccac ccaagtgttg taatgtggtc tattgcaaat gaaccagatt
      421 cggattccga aggtgctaaa gaatattttg aacctttagt tgaattaact aaagaacttg
      481 atccgcagaa aagacctgta acaatcgtta cttatctaaa atcaacgcca gatgtttgta
      541 aggtaggaga tattgtcgat gttttatgtt taaatcgtta ttatggttgg tatgtttcgg
      601 gaggggatct ggcacatgca aaatatctgt tgaaaaaaga gctagatgga tggatggaaa
      661 gatgtcctga tacgccaata atgtttacag aatatggagc agatacagta gctggatttc
      721 atgatacagt accaacaatg tttacagaag agtatcaagt ggcttattac gaagcaaatc
      781 acgaagtaat agatacatgt gaaaattttg ttggtgaaca agtgtggaat tttgcagatt
      841 ttgcaacaag tcaagggatt ttaagagtgc aaggaaataa gaaaggaatc tttactagag
      901 atagaaaacc taaaatgatt gcttactatc tacagaaacg atggtcatca atacccaatt
      961 tcagttataa aaaataattt gg
//
LOCUS       DACECZ010000260          977 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.272, whole genome shotgun sequence.
ACCESSION   DACECZ010000260 DACECZ010000000
VERSION     DACECZ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.272"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            346..977
                     /locus_tag="IWO35_002821"
                     /pseudo
     CDS             346..977
                     /locus_tag="IWO35_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          332 a          180 c          178 g          287 t
ORIGIN      
        1 tagttatata tttaaatttt taccagaaag aaatgaacac cttcaattat ctaaatctcg
       61 aaaatggtta gatgtaatca tcaggaaaaa caatcttaaa cgaattacta ttcatggtct
      121 tagacacaca catgctagct tgcttttagc aagctagtgc aaatattaaa gatgtgcaag
      181 agcgtttagg ccactcttct attcaaatca ttatggatct atatattcat attacagaca
      241 aaagaaaaga agaaaccgta gcgcaattcg caaaatatag gctctataat ttatgggact
      301 gatgaatcaa aattgattct tcagtcccat tgttatttta ggtatatgat tcgtacattg
      361 aaagagttga aaaactatct tccgaagccg tcaaaaagga ccctgccaaa agtattgatg
      421 gtggatgaat ttcgttcgca cgcttcaatt gtggataaaa tgagctttat ttgtgcggat
      481 ggcgagactg gaaaactcat tgatattttg cctacacgaa aattgtctcg attaacacac
      541 tattttagtc gctgtgcaaa tcctgaagcg gtggaatatt tagtgacaga tatgaacgcc
      601 gcctacttcc aactgacaaa acgtgtctta ccaaatgcga aagtcgtcat tgattgtttt
      661 catatcatca aacatttgaa tcaggcgttc aatgattttc gtgtacaaga aatgaaaaga
      721 ttacaccagt tagggcaacg acgggaagcc gagaaagtga aaaagaactg gcgctttctt
      781 ctaaaaaacc gtcaaaaaat caatcactat gattacaaaa catggcgcag ttttcgtgca
      841 cctaaatacc cgctactcac tgaagcaatg atgattgatc gattgctggc attttcaacg
      901 cctttaaagg acgcctatac ttttttccat gagctgaccg aagcctttag agacaaggaa
      961 tcagacttat tttttac
//
LOCUS       DACECZ010000261          967 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.273, whole genome shotgun sequence.
ACCESSION   DACECZ010000261 DACECZ010000000
VERSION     DACECZ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.273"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(210..455)
                     /locus_tag="IWO35_002822"
     CDS             complement(210..455)
                     /locus_tag="IWO35_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0317804.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(449..850)
                     /locus_tag="IWO35_002823"
     CDS             complement(449..850)
                     /locus_tag="IWO35_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0317805.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT          310 a          156 c          135 g          366 t
ORIGIN      
        1 ctataaaaat tattttagaa tgtttatcgc tccttcttta cagtaagaaa tcatggcgca
       61 actaaaatat gctccttgaa gtgttaacgt actgatagaa tgtatttaaa tttcaatgat
      121 caataggttt tctgtttaaa agaatctact aatcctttaa ctattagaag attcttttaa
      181 acagaataag tggaaagggg actttttagt tagttaattt taaattttaa gatcgcttct
      241 atcaatttaa tttctagctg tcttagcata tactcatcta cataggtaat aaattgcccc
      301 gcctcattaa atccatttgt caaacataac ttagctatat aactatgata atgtttgagt
      361 atcatcttga ttgaaattgg atcttcttct agagcagatt ggataatgga tagaggaact
      421 aatggatact gccgtttttt ggggttgtct accataagtc ataccccatt tttaattgtt
      481 caactgcctt ctttcgttga taccagatac cacttgtact catattgagg attttaccga
      541 tttcagtatc attaaaacct gcaaaataat aaagtaggat aacctttctt cttatttccg
      601 ataaatgatc aatggctgcc gataactttt gatctgaaat taatatttcc attccaaata
      661 agaaatagac atcttgttca tcaaaatgag tttgggagta cttaccttca tttgaattcg
      721 tcaatggttc ctcattatct gttatgttaa tgtttcgttt tcgaatagac ttagtttcgt
      781 ttcgtaatac ttttttacag taggcatcaa attgatgttg tatagatttc tcattgtaat
      841 tttgttgcat gataaatatg ctcctttttt cccctttcca ctaatacaac aatcagaaag
      901 cgcctttttt tcgaaaaaac tagaaaaaaa tatccttctt taaacagaaa atctttttat
      961 aaaaaaa
//
LOCUS       DACECZ010000262          927 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.274, whole genome shotgun sequence.
ACCESSION   DACECZ010000262 DACECZ010000000
VERSION     DACECZ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.274"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..903
                     /locus_tag="IWO35_002824"
     CDS             <1..903
                     /locus_tag="IWO35_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ0317806.1"
                     /translation="KDSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLN
                     CYQGKVKTILNELETYNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDD
                     DLSETIDKSTSSRNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTETDQ
                     LQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKHKAEEL
                     EKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEKAIA
                     AKLANEQRETFNELPTVSTENWLE"
BASE COUNT          392 a          145 c          155 g          235 t
ORIGIN      
        1 aaagacagct caaaaatagc ctatatgctt tttaaaaatg aatttcgtag agccttacat
       61 gaaaaatgga tcgacgatga tggtctatta tatttggaat ttacacaagc agacctaatg
      121 gacaaattaa attgctacca aggcaaagta aaaaccattt taaacgagtt agaaacatat
      181 aatttgattt cagtcgtaaa aggagaattt aacccaaaaa cgaaaaaaaa tgaaaaaaat
      241 aaatactatt tacttcaacc tgaaataagt gccgatgatc tttttattca aaattctttc
      301 gatgacgatt taagcgaaac tattgataaa tcaacctctt ctcggaatgc ggaaatcgca
      361 cgtcgagaaa aaacagctga aacccttgat aacactggaa atgcggaaat cgcacaagat
      421 tattatatta ataaatcttt agacactaaa agacacttaa aagacactga aacagaccaa
      481 ttacaaaatc aagttttgct agataacttc gttgaaatca tgaaagaaga cagcataaac
      541 acatttgtac cagaaaaggt attaagcttg ataaaaacat tttctaacac ttacgaagaa
      601 gcccaacaaa ccgtcaccac aatacacaac gcaaaacata aagccgagga acttgagaag
      661 acggtagttg tgtatgaaga acttgaatct tacggaattg atgcggataa aggattatat
      721 atgacgttgc tgaaagctta tcaaaaacaa aagactgaga aagtaaataa tttgcagaat
      781 ttgatttttg tgtatgtaaa aaactggttt attgaaaaag ctattgcggc aaagcttgca
      841 aacgaacaaa gagaaacatt taacgaatta ccaacagtta gtacggaaaa ttggctagag
      901 tagttgataa aaagcgaaca ctataaa
//
LOCUS       DACECZ010000263          891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.275, whole genome shotgun sequence.
ACCESSION   DACECZ010000263 DACECZ010000000
VERSION     DACECZ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.275"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(134..682)
                     /gene="aac(6')"
                     /locus_tag="IWO35_002825"
     CDS             complement(134..682)
                     /gene="aac(6')"
                     /locus_tag="IWO35_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0317807.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          253 a          190 c          136 g          312 t
ORIGIN      
        1 acctctggta cacggtgttc caaaagtagc ttcttcggaa ataagttgga atttcacaaa
       61 aattcaagaa gtaattttcg taaattcctt cttatttctt gaagctgatt acttctgtca
      121 caacctcttt ttattattga gaatttggtc gaggaataat cgtttttgcc atccaaatat
      181 ccggtttgtc ccagccattt gcatttggta agacacctac gattttataa cctaattttt
      241 catagaattc atacggatgt tcacgaaggt tctggatgga agccacttta tcaaatgtat
      301 gctcatacag gtccgtttga cttaacgttg ttccatggtc taaatcatcc gtacctaaat
      361 aaatcgtgat tcctcctctg gaagctactt ctttttctaa gtaattgact aatcgagtac
      421 ctatttggtt ctttcgtcgg gagctttcta caactaatgg atgcaattcc caacctgtga
      481 taccgtattg agggattgca ccaataaatc ctactaactc atcttggtct accgctgcta
      541 ccgcgattcg ttctggattc atcatttctt ctacttcttc tgccgagctg tctccatatt
      601 cttccggcca agtcagtctc agtaaatcag aaagctgatc tttcaatact ggattattac
      661 ggtcaaattc actgattatc atacgtcttc ccccttgttc ttgcttattc taacagtcct
      721 tttgaaaaaa cgaaatgttc tcgctcaaaa atctttagct accaatcagt atctctattc
      781 tttcattatt tatcttactt ataaaataaa aaaattatac agaaacactt tattcaaaat
      841 aggaaagatc aggaaagcca tcaattggag gaaaaaactc tgtttgttca t
//
LOCUS       DACECZ010000264          890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.276, whole genome shotgun sequence.
ACCESSION   DACECZ010000264 DACECZ010000000
VERSION     DACECZ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.276"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..888
                     /locus_tag="IWO35_002826"
     CDS             <1..888
                     /locus_tag="IWO35_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ0317808.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DACECZ010000265          887 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.277, whole genome shotgun sequence.
ACCESSION   DACECZ010000265 DACECZ010000000
VERSION     DACECZ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.277"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..533
                     /locus_tag="IWO35_002827"
     CDS             <1..533
                     /locus_tag="IWO35_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ0317809.1"
                     /translation="SIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAFKSMGGGVELD
                     CIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIEIHLDYFGRVP
                     TRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFFENVVTGKENW
                     NDLYHANEVLKIAEEK"
     gene            534..>887
                     /locus_tag="IWO35_002828"
     CDS             534..>887
                     /locus_tag="IWO35_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAQ0317810.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLR"
BASE COUNT          339 a           96 c          160 g          292 t
ORIGIN      
        1 atagtattca aagtaaaaaa gtatattctg taagagctat ttgttctact tatcttcctg
       61 attggagaaa aggtactgat tatagaaata attattctgc ctttaaatct atggggggag
      121 gagtagaatt agactgtata catgaattgg attatattat aaacctattt ggtttcccta
      181 aatcagtaaa aaaacagttt ggtaaatatt cgaatcttga aattaacagt aatgatttag
      241 caacatatat attgagatac gatgataaaa ttatagaaat tcatttagat tattttggaa
      301 gagttcctac aagaaaattt caattgttta ctttagatga atgtatcaat gttgacttat
      361 taaataataa aataagtttt gaagaaaaca ttgttaaaga aatgcctgaa tctgctaatg
      421 atatgtatga gaaggagttg aaatatttct ttgaaaacgt agtaacaggc aaagaaaact
      481 ggaatgattt atatcatgca aacgaagtat tgaaaatagc tgaggagaaa taaatggaaa
      541 ttttatttac tatttgtggt agagctgggt ctaaggggtt taaaaataaa aatttgaaat
      601 atttggcagg aaagccccta gtccatttcg ctttagcaat gatagatatc tatcaacaaa
      661 aacatcctga acataattgc acgacttgtt tgaatacgga cagtaatgag ttagtagaat
      721 tgacgactaa gttaaatccg tctgtgaaaa ttgtagaacg taaacctgaa ttagctacag
      781 atactgtagg gaaaattgaa gtgatacgtg attcattctt acaactagaa agagaagata
      841 atatatttga tttaattata gatttagatt tgacttcacc tttacgt
//
LOCUS       DACECZ010000266          863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.278, whole genome shotgun sequence.
ACCESSION   DACECZ010000266 DACECZ010000000
VERSION     DACECZ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.278"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>863
                     /locus_tag="IWO35_002829"
     rRNA            <1..>863
                     /locus_tag="IWO35_002829"
                     /product="23S ribosomal RNA"
BASE COUNT          214 a          188 c          272 g          189 t
ORIGIN      
        1 atgcaggtta cccgcgacag gacggaaaga ccccatggag ctttactgta gtttgatatt
       61 gagtgtctgt accgcatgta caggataggt aggagccgta gaaatcggaa cgctagtttc
      121 gatggaggcg ctggtgggat actacccctg cgttatggcc actctaaccc gcaccactaa
      181 tcgtggtggg agacagtgtc agatgggcag tttgactggg gcggtcgcct cctaaaaggt
      241 aacggaggcg cccaaaggtt ccctcagaat ggttggaaat cattcgaaga gtgtaaaggc
      301 agaagggagc ttgactgcga gaccaacaag tcgagcaggg acgaaagtcg ggcttagtga
      361 tccggtggtt ccgcatggaa gggccatcgc tcaacggata aaagctaccc tggggataac
      421 aggcttatct cccccaagag tccacatcga cggggaggtt tggcacctcg atgtcggctc
      481 gtcgcatcct ggggctgtag tcggtcccaa gggttgggct gttcgcccat taaagcggca
      541 cgcgagctgg gttcagaacg tcgtgagaca gttcggtccc tatccgtcgc gggcgttgga
      601 aatttgagag gagctgtcct tagtacgaga ggaccgggat ggacttaccg ctggtgtacc
      661 agttgttctg ccaagggcat tgctgggtag ctatgtaggg aagggataaa cgctgaaagc
      721 atctaagtgt gaagcccacc tcaagatgag atttcccatt tctttaagaa agtaagatcc
      781 ctgagagatg atcaggtaga taggtcagga gtggaagtac agtgatgtat ggagcggact
      841 gatactaatc gatcgaggac tta
//
LOCUS       DACECZ010000267          855 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.279, whole genome shotgun sequence.
ACCESSION   DACECZ010000267 DACECZ010000000
VERSION     DACECZ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.279"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(100..300)
                     /locus_tag="IWO35_002830"
     CDS             complement(100..300)
                     /locus_tag="IWO35_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0317811.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          281 a          146 c          131 g          297 t
ORIGIN      
        1 ttttatacac tactattcag cccattcaac gaccaatgaa ttttttcgta caaaaaaacc
       61 gtcttttctt tcgaaaagac ggttaaaagt caatttttat taagctttaa caatgtttgt
      121 tgcttgaggg ccacgttgac catcttcaac atcgtaagac actgcttggc cttcttctaa
      181 agttttgaag ccgtcacctt ggatagctga aaaatgtgca aatacatcat ttccatcttc
      241 accagtgata aagccaaaac ctttgtctgc gttgaaccat tttactgtac cgttattcat
      301 aaataaattc ctcctgatac gtatcaatgt acgttttttc gttgcaataa atatttgata
      361 ggcaaaagga gttttatcta tgtagatata tcgctcaaat tacgtttcaa aatagattac
      421 tcatttaata tatcatgctt tttctaataa cacaaggata atgcaatgaa atataaaatt
      481 cattgtcttc tattgtcttc gaatcgaaga aaaagttgaa taaccgcgat tgattcactt
      541 tttgttttct tgtttatttg cttttatctg caacttttga ctttaaaatt tcttaactat
      601 gaaaaccttt caattggtgg tatacttata aatggaattt cgttatcaac gcgttcgttg
      661 cctttcagca agtatttaaa gaaaggagaa tggctatgac attctataac aaagatatac
      721 aatatcgagt aaccttggat acagatttga atctatttgt cgtttttgac aaagaaaata
      781 ccaatcatgt agcgacaggt gttacgatcg aacaagcagt gcaagaatta aaaaaatctg
      841 cataaggagt ggaaa
//
LOCUS       DACECZ010000268          841 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.281, whole genome shotgun sequence.
ACCESSION   DACECZ010000268 DACECZ010000000
VERSION     DACECZ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.281"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..841
                     /locus_tag="IWO35_002831"
     CDS             <1..841
                     /locus_tag="IWO35_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAQ0317812.1"
                     /translation="ESFSIVHNQVYFGGGINLWLAKKNGIRQRIAHSHATEDGKSQNI
                     VMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNSEAKVN
                     KRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRNKLK
                     AKAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLAD
                     TISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTEQMESIYS
                     RDH"
BASE COUNT          324 a          101 c          162 g          254 t
ORIGIN      
        1 agaatctttt tctatagttc acaatcaggt ttattttggc gggggaatta atttatggtt
       61 agccaaaaag aatggaatca gacagagaat tgctcatagc catgcaactg aagatggaaa
      121 atctcaaaat attgtaatga atgtattgcg taaattttta accaaattgt tgttacaaaa
      181 tgcaacagac taccttgccg ttagtcaaga agctggtgaa agtctgtttc aaaaccatcc
      241 ctttgagata gttcataatg gtattgattt agagctttat agtaaaaatt cagaagctaa
      301 ggttaataag agaaaagaat tggatatctc aatgagtact tttgttgtgg gaaacattgg
      361 acgattagag aaacaaaaaa atcaatctta tttgttagag atttttttag aaatactaaa
      421 aatgaattct gattcttact tgattttagt aggtgaaggg tcacttagaa ataaactgaa
      481 agccaaagca gtagaattgg atatagagaa aagagtaaaa tttttaggag aaagaaacga
      541 tattcccgag ttattatctg tgatggatgt ttttgtaatg ccttctcttt atgaaggtct
      601 gccaatggtt ggattagaag cacaagcagc aaaattaaaa ttagtcttag cagatactat
      661 ttccaaagat acaaagttaa caaataatgt ggattttgta tcattagatg aaacaccaca
      721 agaatgggca aaaaaagtaa taaaagagcc gtatgctaat aaaataacac aagaactaaa
      781 gagttatgat gtttcgtaca ctactgaaca aatggagagt atatatagta gagatcatta
      841 a
//
LOCUS       DACECZ010000269          827 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.282, whole genome shotgun sequence.
ACCESSION   DACECZ010000269 DACECZ010000000
VERSION     DACECZ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.282"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            139..>827
                     /locus_tag="IWO35_002832"
     CDS             139..>827
                     /locus_tag="IWO35_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ0317813.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHF"
BASE COUNT          285 a          117 c          175 g          250 t
ORIGIN      
        1 gagctctagg tgcagtttat ttaaatggta tttgggaagc gcttgaaaag ggggcgagta
       61 aataattata taagggagtt tatttagagg aaaaattttc tcttctaaaa actaacggaa
      121 ggaaaggagt gtttaaatat gtttttagat aagaactttt tattagagaa tgaaatagct
      181 aaaagacttt ttcataaatt tgcaaaacac caaccaatta tcgactatca ttgtcattta
      241 gacccagcat atttgctaga aaataagcaa tacaagaatt tgactcaggt atggttaaat
      301 gataaaggag ctggagatca ttataaatgg cgattgatgc gagctaatgg tatagaagaa
      361 gagttgatca gtggtccagg tgatgattat gaaaaatttt tagcttttgt aaaaacagtg
      421 gagaaggcgc caggtaatcc tatttttgaa tggtctcatt tggaattaca acgttacttt
      481 gatattgatt tgacaatttc tgagaagaat gcaccagaaa tttggcgacg tgctaacgaa
      541 aaaattcaaa cggattccta tcgcccgaaa gaattgttgg agatgatgga tgtgaaagcc
      601 atctgtacaa cggatgatcc tacagatgac ttgtggtatc atactgagct ttctaaacag
      661 gataattctt tcaaggtatt acctactttc cgtccagata aggtctggag tatcaaatca
      721 tcaggctttt cgaactatat tttaaaactt agtcaaacag cggctatacc aatcaaagac
      781 tttaatgggt tgaagcaagc attgagccaa cgagtggatc attttca
//
LOCUS       DACECZ010000270          819 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.283, whole genome shotgun sequence.
ACCESSION   DACECZ010000270 DACECZ010000000
VERSION     DACECZ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.283"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          317 a          117 c          130 g          255 t
ORIGIN      
        1 aggcaatctt aattttttaa attgtctaaa ttatatagaa agaacaatct ttctatataa
       61 ttagacataa gagagactac ggttgatttg atattaacaa gcgtatatga atgtatatac
      121 aaataaaaaa ggggagggag aagttaaaga aaattcagag aagtggggca ctcagtagta
      181 ttaacaattt aaaaggaatt tcttaaagaa aatgggttgc atcctggaga tacaattttt
      241 ttagataaag aaaaaattca agcaacaatt caaaaaaatc ttaactgctg agattgatgt
      301 agcaatatct caatcatcaa caaagtacga tcctgccttt caaaagctta ttgataaatg
      361 tttattcatt ataaaaataa aagaaaaatt tatgtcagct tttttcatca aagtggtaat
      421 gataattcgt ctattaattg tattttttag gaaatatggt ataattgttt taacgaaaag
      481 gtaattcatg atacaccaaa aaccactagc tggcgggcta gtggttttct tttacgttct
      541 attcagatta acgggtaggc ttactgtctt ttttggcaat aattttgcaa tcaagccatt
      601 tacagatgta atgcgcggat acttctgcta agattgcaaa aaacatatcg aaaaggtaat
      661 tcataataca acctcctccc cttgccataa taggccgggg tagcagacag cataaatatt
      721 ataccataac tgaaaatttc attttagtac atttcaatat attgatgaac cagcacaata
      781 aaacatgcca tgactaacag aaaaaaactt attaaacca
//
LOCUS       DACECZ010000271          815 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.284, whole genome shotgun sequence.
ACCESSION   DACECZ010000271 DACECZ010000000
VERSION     DACECZ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.284"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>815)
                     /locus_tag="IWO35_002833"
     CDS             complement(<1..>815)
                     /locus_tag="IWO35_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0317814.1"
                     /translation="SIDTHLHLSAAPYFRLLINESANLYAEFAHKSIELDNPSLLFID
                     KQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFSIDQEITSIITFLKNEQFKID
                     YLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCLQKIQLSSLPRHYQQELALLT
                     IPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLLEQLAQIFFISPASLKKHFRK
                     VNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGFHSIHHFSSAFKKYTG"
BASE COUNT          281 a           98 c          141 g          295 t
ORIGIN      
        1 aaccctgtat attttttaaa agctgaagaa aaatgatgga tcgaatgaaa tcctaatata
       61 tctgcaattt ctgtataggt taattcattt tctagcacga gacgttttgc tttttccatt
      121 tttagttctt taaaatagct aataacactt tttccattca cttttctaaa atgttttttt
      181 aagctggctg gactgataaa aaaaatttgg gctaattgtt caagcaaaaa attttctgaa
      241 agatgttctt ctaaatatcg tatgattgga tcaactaatt catctgaata aagaatatgc
      301 ttttttttag gaatgacttt ctcatttgaa ggtattgtca ataaagcaag ttcctgttga
      361 taatgccttg gtaaggaaga caattgaatt ttttgcagac aataagtaac taataaggaa
      421 agcaaatgaa tgctcatctg ttggcatagt atgaaaaaag aattgatttc ctgatcatag
      481 gaaatagctg gcttactatg ttttagataa tcaattttga actgttcatt tttcagaaaa
      541 gtaatgatag atgttatttc ttgatctata gaaaaagatt gtcccgtaat atgtgctaat
      601 aattgctcat caaaataaaa ctggataatg ataaaagttt gtgtttcctt ttgagtagaa
      661 aagatttgga cttcttgctg tttatcaatg aataaaaggg aaggattatc tagttctata
      721 gatttatgtg caaattcagc atataaatta gcgctttcat ttattagtag tcgaaaatag
      781 ggagcagcag ataaatgaag atgggtatct atgga
//
LOCUS       DACECZ010000272          791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.285, whole genome shotgun sequence.
ACCESSION   DACECZ010000272 DACECZ010000000
VERSION     DACECZ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.285"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..782
                     /locus_tag="IWO35_002834"
     CDS             <1..782
                     /locus_tag="IWO35_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0317815.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          242 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc t
//
LOCUS       DACECZ010000273          765 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.286, whole genome shotgun sequence.
ACCESSION   DACECZ010000273 DACECZ010000000
VERSION     DACECZ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.286"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            23..>765
                     /locus_tag="IWO35_002835"
     CDS             23..>765
                     /locus_tag="IWO35_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ0317816.1"
                     /translation="MQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDYEKYDRTGF
                     NSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEK
                     GVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDATYIP
                     LKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLLFI
                     TDGLKGMKDTIHQIYPKAKYQHCCIHVSR"
BASE COUNT          273 a          141 c          132 g          219 t
ORIGIN      
        1 gaatgatttt actacagaaa ttgtgcaaac tctagtcact aaaggcgatt taaatgaatt
       61 attccgttcg cacttagaaa aagcgataaa cacactccta cggactgaat taacggcttt
      121 tttagattac gaaaaatatg atcgcactgg ttttaattca ggtaattcga gaaacggttc
      181 ttactttcga tcaatcaaaa ccgaatatgg tgaattaaca ttggaaatac ctagagatcg
      241 taatggtgag tttaaacaac aaactttacc agcctacaaa agaacaaacg atacattgga
      301 aaccactatt atccatttat tcgaaaaagg tgttacgatg tctgaaattg ctgatttgat
      361 cgaaaaaatg tacggtcatc actatactcc acaaaccatg tccaacatga ctaaagttct
      421 gactgaagaa gtaaatgcct ttaaatccag agccttaaat gataagtatg tcgctatttt
      481 tatggacgct acttacattc cactaaaacg tcaaaccgta tccaaagaag cgatttatat
      541 tgccattggt atacgagaag acggcactaa agaagtactg agttatgcga ttgctccaac
      601 tgaatcaaca tacgtttgga atgagctgct acaggatatt aactccagag gagttcaaga
      661 agtcttgctt tttattacgg acggcttaaa aggcatgaaa gatactatcc atcaaattta
      721 tcctaaagca aaatatcagc attgttgtat ccatgtatct cgtaa
//
LOCUS       DACECZ010000274          735 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.287, whole genome shotgun sequence.
ACCESSION   DACECZ010000274 DACECZ010000000
VERSION     DACECZ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.287"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            107..>735
                     /locus_tag="IWO35_002836"
     CDS             107..>735
                     /locus_tag="IWO35_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317817.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETD"
BASE COUNT          237 a          113 c          163 g          222 t
ORIGIN      
        1 ttttttgtgt gatttatttt cttttctaat tcaacctgtg gtatgatgag agcataagta
       61 tcatgtgaaa tgatatgaag tatgtggaag atggacgggg aagaatatgg aagcaaaaat
      121 cactttagaa ccttttgaac gtatcttaag cggttaccgc aaagtagaag agttagctgt
      181 gaacgtaact gattgttcaa aattagcgca aaaatacgca agattcggcg ttgaaggcta
      241 tcgattagga aactatgtcg gtacagggta tctgaaccgt tatttagagt gtatggtgga
      301 ccgcgcacca atgctgattt atagacaaaa ttatctgatt ccgctgcttt ttcgtcgttc
      361 tgattcagcc tttcggttat ttgaagaaga gtatcggatg gaggctttct ttctactatt
      421 agagtggagc ttgaaacatc gtcctgaaaa aattcttatc gaaagaaatg agaagataga
      481 cacgaaaaaa aataaagtga tcgatagtgc atacctagca tttcgtgtat cagagatttt
      541 ggattgtggc ggctatccaa tcagcaattt ccaatcaatt gaccaattca ttgaatggaa
      601 tcgaatttac cgattgatcg acaatggcgg gattggccgt catagcaagg tatttgaccc
      661 agaatatcca gaaaacatgg aagaactgaa aatgattatt tcgcttgtca aattaaaata
      721 tcctgaaaca gattt
//
LOCUS       DACECZ010000275          733 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.288, whole genome shotgun sequence.
ACCESSION   DACECZ010000275 DACECZ010000000
VERSION     DACECZ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.288"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(131..685)
                     /locus_tag="IWO35_002837"
     CDS             complement(131..685)
                     /locus_tag="IWO35_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0317818.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          218 a          147 c           75 g          293 t
ORIGIN      
        1 ttgttctatc tctggtattc tattgtaaat gttactaaac gttttaaata cttccgaaaa
       61 ttcagttatc ttcattaatc tcaccttcta agttttcatt atacacaaaa aatatatcct
      121 aatatctcaa ttagcctccc aatttaaaac cacgtaacga tgcccctatt tcatcttcag
      181 ttataccaat gtaccttttt gtaattgctt gcgagctatg attaaaaata aacattaagg
      241 tagcaatgtc tctagttttc ttgtagtagt gatacccaaa agtttttctt aatgtatgtg
      301 ttcctatatc atctcttcct aatttttctg ctatatctgc atatatctga tagacacgat
      361 cccttgttat atgtcgacct tgttgccgac ttggaaataa ccaatcattt tcttctttac
      421 cttcagtata taataaaatc tcttctctca aattatctat aaaaatcggt tttcgcttac
      481 ctgttttatg ttcaacaata ataggcttac ttttacctcg tatgtctcca acttttaatc
      541 ctataatatc tgacatacgt aacccattat tgattccaaa caaaaataaa aaactatctc
      601 tttctggatt acttgttctt cttagccaaa actgaaaatc ttgtatctct tcatttgtcc
      661 ttaacggttg tacattatag ctcactattg tttcccttct taaaaatatt ctgagttcat
      721 gattatatat ata
//
LOCUS       DACECZ010000276          732 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.289, whole genome shotgun sequence.
ACCESSION   DACECZ010000276 DACECZ010000000
VERSION     DACECZ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.289"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            94..462
                     /locus_tag="IWO35_002838"
     CDS             94..462
                     /locus_tag="IWO35_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ0317819.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT          260 a          120 c          112 g          240 t
ORIGIN      
        1 gttacaacat tacttgtgtt ttgttcgttt tttatacata actattgttt ttctggtaaa
       61 ataaatcaaa ccacttaatg gaggcgtttt agaatggaaa aatttcagcc gatcatcaat
      121 cagatcaaag aaaaagaaca tcaacaaaag atcaaagacc tttttgctaa gataacagaa
      181 acgtatcccc agctagacac aactgtaaaa tggaaccaac ctatgtttac tgaccatggt
      241 acctttatta ttgcatttag ttttgctaaa aatcattttt ctatcgcacc ggaaaaagca
      301 gcgattcgtg cccttgaaaa aaatatccaa gaagcaggct acgtatatac ggataacgtc
      361 atcaaagttc cttggaaaag tgtaatcaac tgggaattga ttgaacaatt gatttcattt
      421 aatattgaag ataaaaaagg tcatgataaa ttttggcgat aaagctttat acgaataaac
      481 cttttactgg cgaggaaact attcatcagc ttatacaggt tttgttcaat cctatccttt
      541 ttatcatccc tggttaaaca cttaattgac aaagtatctg ctatttttat aaggagtctt
      601 tcttgtgctg aaaaaaatca ctgattgaat ggctttgaat tttccactta gtaaaattat
      661 tttttcagca attttcctgc aaaacttttg aataaggaaa aaatacgaga aaatatcagt
      721 gatctttcat aa
//
LOCUS       DACECZ010000277          705 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.290, whole genome shotgun sequence.
ACCESSION   DACECZ010000277 DACECZ010000000
VERSION     DACECZ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.290"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            47..448
                     /locus_tag="IWO35_002839"
     CDS             47..448
                     /locus_tag="IWO35_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0317820.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            442..687
                     /locus_tag="IWO35_002840"
     CDS             442..687
                     /locus_tag="IWO35_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0317821.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
BASE COUNT          266 a          102 c          126 g          211 t
ORIGIN      
        1 tctgattgtt gtattagtgg aaagggggaa aaaggagcat attatcatgc aacaacatta
       61 caatgacata tctatacaac accaatttga tgcctactgt aaaaaagtat tacgaaacga
      121 agctaagtct attcgaaaac gaaacgctaa cataaaggat aatgaggaac cattggcaga
      181 ttcaaatgaa ggcaggtatt cccaaactca ttttgatgaa caagatgtct atttcttatt
      241 tggaatggaa atattaattt cagatcaaaa gttatcagca gcaattgatc agttatcgga
      301 gataagaaga aagattatcc tactttatta ttttgcaggt tttaatgatg ctgaaatcgg
      361 taaaattctc aatatgagca caagtggtat ctggtatcaa cgaaagaagg cagttgaaca
      421 attgaaaatg gggtatgacc tatggtagat aaatctaaaa agcggcagta cctattagtt
      481 ccactatcca ttatcttatc tgctctagaa ggagatccaa tttcgatcaa gatgatactc
      541 aaacattatc attcttatat ggctaagtta tgtttgacaa atggatttaa cgaggtaggt
      601 caattcgtta cctatgtaga tgaatatatg ataagtcagc tagaaattaa attaatagaa
      661 gcgatcttaa aatttaaaat taactaatta aaaaaatccc ctttc
//
LOCUS       DACECZ010000278          689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.291, whole genome shotgun sequence.
ACCESSION   DACECZ010000278 DACECZ010000000
VERSION     DACECZ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.291"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(51..>689)
                     /locus_tag="IWO35_002841"
     CDS             complement(51..>689)
                     /locus_tag="IWO35_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HAQ0317822.1"
                     /translation="ELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHG
                     LDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKC
                     LCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILR
                     MYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          232 a          117 c           46 g          294 t
ORIGIN      
        1 aactatttaa tactaatgtc ttttataata gcttttcata tattatataa tcaatcttta
       61 taagtccttt tataaatttc ttttctacca ttttcgataa attcctgttt aatattttta
      121 attccataaa caatagtttc aataggataa tattcttcaa ctatatcttg atattctttt
      181 gctttctcaa tatctatatt tccatacatt cttaatatat cttctccaaa atttgttcct
      241 atttcttctt cactatcttc aagtaagtat ataaaatcac aatattcatc tataattcca
      301 gaatctccaa aatcaattat tccagttaat ctattattgc catctaacaa tagatgatta
      361 caactaaaat cattatggca taaacacttt ttaccctcaa aaactgttgt tgcatttagt
      421 ctttccataa aactttctat ataatctttt tctatatcag ttaaatcatt ataaatagtt
      481 tcacgcaaca atatatactc ttctaataca ttttgtttat tatcaatagt acattcacta
      541 atatctgtat aatctaaacc gtgcatttgt cttaaaaaac tggcaatatc tcgttttaac
      601 aaattttgtt cttcttctga catagtagaa taaatttctg gtgttaaaaa agttccttta
      661 atttctttat aacctagtat agataattc
//
LOCUS       DACECZ010000279          665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.292, whole genome shotgun sequence.
ACCESSION   DACECZ010000279 DACECZ010000000
VERSION     DACECZ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.292"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            16..>665
                     /locus_tag="IWO35_002842"
     CDS             16..>665
                     /locus_tag="IWO35_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAQ0317823.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLAL"
BASE COUNT          235 a          119 c          112 g          199 t
ORIGIN      
        1 aaatgaggac tacatatgcg ttcatcaaaa tatactgaac attatacaga tacttttata
       61 acattcaagg aaattatgag aactgtttct aacatatacc acaattgtgt accagataag
      121 attaaaaacc gaagaaatac tgatcaactg aagcaacgcg atacagtgat tattgcttgt
      181 gttatatggg gtataattaa tggctatact agccaaagag ccacgtatag agcggtttgt
      241 tccgtcttat ttcctaatgg tgactttcct agtaggagtc gattcactcg cttaagttca
      301 aacttagctt acactatcaa gattattcga tactttttca tcaagaagct caccaaaggt
      361 gaactagtcg gaattataga tagttttcct agtccattat gtaaaccagt tagaaataga
      421 caagcaaaac tattgaatca aatagccaaa gttggctata actcaacaaa aaaatcttat
      481 ttttatggtc ttaaaattca tatgatcgtt actaagactg gctttccgat tacttactca
      541 atcacgaatc caggtgtcca tgacgtgaaa gtgttagaga ctttatcgga agaagcgaat
      601 cttcccaata tcctagggga caaaggatat attagccata aaatccatga aaagcttgcc
      661 cttaa
//
LOCUS       DACECZ010000280          653 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.293, whole genome shotgun sequence.
ACCESSION   DACECZ010000280 DACECZ010000000
VERSION     DACECZ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.293"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            110..>653
                     /locus_tag="IWO35_002843"
     CDS             110..>653
                     /locus_tag="IWO35_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0317824.1"
                     /translation="MYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILP
                     LILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEI
                     FLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSID
                     SELLSFGIGLTLLICLIILTF"
BASE COUNT          205 a           90 c           95 g          263 t
ORIGIN      
        1 aactctatat cctttatttt tattgatcaa gtttactaat ttagcaatag gctataacct
       61 ttttctttat attgttacgc tcattactct atttatatgt cattatgtaa tgtatgaaat
      121 taccaaaaaa catgttacta gctccttttt cgctattata tatacaacat cttcttttag
      181 gagtgtagaa atatttcttc gaggggcaat gggggaatta ctcgcaatgt caatacttcc
      241 tcttatttta ctcgggttta taaagcttta cgactcaaaa aaagaatctt gggttatgtt
      301 ggcgattagc atgactttat taatttacac tcatgttctt tctgtagcta tgactatggt
      361 aatgattata attgctttaa ttattcaatt ttatcggaaa aagaaaatag aaatcttctt
      421 aattattaaa ttaataaaag ctgctatagt tactttactt ctttcactag cgtttatagg
      481 tcctatgatt gagcagacat tgtatcaaga tctgaataga ccttatgtgg atatattaca
      541 aaaaagagca atttatctgg gtaaagagtt tttgattgga agtatagatt cagaattact
      601 atcatttggt attggcttaa ctttgcttat ttgtttaatt atactaacat tta
//
LOCUS       DACECZ010000281          650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.294, whole genome shotgun sequence.
ACCESSION   DACECZ010000281 DACECZ010000000
VERSION     DACECZ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.294"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            312..608
                     /locus_tag="IWO35_002844"
     CDS             312..608
                     /locus_tag="IWO35_002844"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0317825.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLSTVCMKKLDVC"
     gene            complement(531..650)
                     /locus_tag="IWO35_002845"
                     /pseudo
     CDS             complement(531..650)
                     /locus_tag="IWO35_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          217 a          120 c           99 g          214 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttccacggt ctgtatgaaa aagcttgacg
      601 tctgttaaag aatacggtat ccgtccaaag gcttctttta ccaatgaggc
//
LOCUS       DACECZ010000282          648 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.295, whole genome shotgun sequence.
ACCESSION   DACECZ010000282 DACECZ010000000
VERSION     DACECZ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.295"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..351
                     /locus_tag="IWO35_002846"
     CDS             <1..351
                     /locus_tag="IWO35_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317826.1"
                     /translation="YLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLAWATRQSYETLL
                     SLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRLLFTDKATADAA
                     ALLVNGTVEEVVER"
     gene            348..644
                     /locus_tag="IWO35_002847"
     CDS             348..644
                     /locus_tag="IWO35_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317827.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
BASE COUNT          231 a          101 c          151 g          165 t
ORIGIN      
        1 tatttaatca aatctaaaga tggtgattca tattatttct gtgattggtt tgtacgagat
       61 ggcatagtga cgcaagagca aggagaagaa ttactcgctt gggcaacaag acaatcatat
      121 gaaacactat tgagcctata caacggctac gaggttgaga aagagccgtt atgggcaata
      181 aagaatgccg atggaaacta tcttactaaa tgtgctttat ggggaaaaga tggagtaaat
      241 tatagttttg aatgcaatcc atctcatcga ttgcttttca ctgataaagc aacagcggat
      301 gctgcagcat tgttggtgaa tggaacagtg gaagaggtgg tagaaagatg aaactaaaag
      361 acggatttta cgctagcagt cacggtatcg gcggtttaat gctagatatg ccgacaaaga
      421 accctaaaac acgcaagaaa tcaaaattca aagtcggtga catggtccgc tgtgaagcag
      481 aagagttcgt ttatccgttc agaggatacg ttaaaaagat actgtcaaac tcagcaatca
      541 ttcgtattga aaatacaatg aagtgtgatc gttcgactgc aaaacacaag cattatttag
      601 ctgtagcgag attagttgac atggaagtaa tcaagagcaa ataaaaaa
//
LOCUS       DACECZ010000283          645 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.296, whole genome shotgun sequence.
ACCESSION   DACECZ010000283 DACECZ010000000
VERSION     DACECZ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.296"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            18..641
                     /locus_tag="IWO35_002848"
     CDS             18..641
                     /locus_tag="IWO35_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317828.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
BASE COUNT          226 a          102 c          136 g          181 t
ORIGIN      
        1 aaaaagtaga aaagaaggtg tttgttattt gggaagtagt taaatcgaga gtacaagaaa
       61 tgaatgaaca tttctcagtg tcattcgcag aaaatccaag taagtatttt cgacgtcttg
      121 tttttctgct catcttatca ttgctgttaa cagttgcaat gtttttattg ccggaaaaaa
      181 tccgagaaga gcaagtaaat agtctcgttt attcgtccat accaaccagc aaattgaaac
      241 cttttgatta cagcaaagct gatcagaaaa tcaaacaaag ccgggcgata tcggtgatgt
      301 tcagcaaacc gaacgggaaa acctatcagg acatgcttga tgtttttaat gatcctgagc
      361 ttatggaaga attgaatcga ccgatctttt attatccgat cgtttatgat gtacatgaac
      421 tggaacaaaa atataacata cgaacggatg aagtgacatt tattttcttt gatggaggaa
      481 aagaagcaaa tcggataact gcgggtaagg gaggaataac tgattttgat aaggagttga
      541 tccctgaact gaatcgactt ccgttagcca atatcaaaca attagaagaa gaattggata
      601 gaacgggagt aacttcccaa aattccgaga ttgctaattg agaca
//
LOCUS       DACECZ010000284          640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.297, whole genome shotgun sequence.
ACCESSION   DACECZ010000284 DACECZ010000000
VERSION     DACECZ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.297"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>640
                     /locus_tag="IWO35_002849"
     CDS             <1..>640
                     /locus_tag="IWO35_002849"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0317829.1"
                     /translation="NKVAGTKKINKINSITGQSLNFSYNKRKVLSNISFSTEMNKLFM
                     ISGENGTGKTTLMKLFTKQLPVSNNQLFVNGIDINMIDNESLRRLISIATQIPYIINS
                     SVKENILMGTKNYDKKKFSKLIELLGISKFMTKLGITLDDNIYDEKILLSGGEKQKIS
                     LARALIKSASVIILDEPFRNLDNETRDNLRDYILNTKKEVCYIIITHDHILDN"
BASE COUNT          282 a           68 c           97 g          193 t
ORIGIN      
        1 aacaaagtgg ctggcacaaa aaaaataaat aaaataaatt ctattactgg tcaatcatta
       61 aatttttcat ataacaaaag aaaggtatta agcaatataa gtttttcaac tgaaatgaat
      121 aaactattca tgatatcagg agaaaatgga acagggaaaa caacactgat gaaattattt
      181 actaaacagt tgccggttag taataatcaa ttattcgtca acgggataga tattaatatg
      241 attgataacg aatcattaag aaggttaatt tcaatcgcaa cacaaatacc atatataatt
      301 aatagctccg tgaaggagaa tattttaatg gggactaaaa actatgataa aaaaaaattc
      361 agtaaattaa tagaactatt gggaataagt aaatttatga cgaaattagg tattactttg
      421 gacgacaata tttatgatga aaaaatatta ttatctggag gagaaaaaca aaaaataagt
      481 ttagctaggg cgcttattaa atcagcttca gttattatat tagatgaacc atttagaaat
      541 ttagataatg aaactcgcga taatttgagg gattatatac taaatacaaa aaaagaagtt
      601 tgttatataa ttataactca tgatcacatt ctagataatt
//
LOCUS       DACECZ010000285          639 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.298, whole genome shotgun sequence.
ACCESSION   DACECZ010000285 DACECZ010000000
VERSION     DACECZ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.298"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..220)
                     /locus_tag="IWO35_002850"
     CDS             complement(<1..220)
                     /locus_tag="IWO35_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HAQ0317830.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLI"
     gene            complement(213..>639)
                     /locus_tag="IWO35_002851"
     CDS             complement(213..>639)
                     /locus_tag="IWO35_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ0317831.1"
                     /translation="KFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDE
                     KFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERD
                     IDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
BASE COUNT          228 a          106 c           69 g          236 t
ORIGIN      
        1 caatcagtag ccaagaaaaa cgcattttaa aaaaattttt ctttataaaa acctcataca
       61 aaactataat tccaccccaa gcaaaagcta ttttcatcgg gatattagtt acattaaaag
      121 tactaggcat aaaataaagc agacaaatga ttaaatttat tactcgaaac gcatctcgac
      181 ttaataaact cttctctaaa acaggtacta ttctattcat ctatatttcc aactttcaaa
      241 atttctttat caattctatt taatagttta tcagagtaat ccgatttaac ttttcttaca
      301 aataattttt cactttcttt tatttgatct atatcccttt catcaaacca tttgttcatg
      361 gaaggatcaa taatatgaat atttgctaat ttcgaagttt gtaaagtagt ttcttcaata
      421 tttttcaatt caagtggtaa atctggtcct ccaataacat ttttattttt gaacgaggaa
      481 ttaaaaaaaa gtgtatgaaa aaatttttca tctggggcaa acatacctat tttaaaattc
      541 aggtagtctt gttgattttg ttcatacatt tgtattaatt ctgttaggca ttctttcgac
      601 aaagcccacc attgagaacc atgatacaaa tcaaattta
//
LOCUS       DACECZ010000286          624 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.299, whole genome shotgun sequence.
ACCESSION   DACECZ010000286 DACECZ010000000
VERSION     DACECZ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.299"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>624
                     /locus_tag="IWO35_002852"
     CDS             <1..>624
                     /locus_tag="IWO35_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAQ0317832.1"
                     /translation="LRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGSSRDKNY
                     LSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGACTDGTEV
                     GVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISSRSFGQS
                     PDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLST"
BASE COUNT          213 a           83 c          132 g          196 t
ORIGIN      
        1 cttacgaaaa aaaccatatc atttagatga agatgctatt tcttggattg aagagacgat
       61 tgatgctatg actttagaag aaaaaatagg tcaattattt gttaatatgg gatctagtcg
      121 tgataaaaat tacttaagta gtatggtcaa tcagtaccat attggagctg tacgatataa
      181 tccaggaact gctgatgagg tatatgatca aaactatatc ctacaaacag aaagcaaaat
      241 tcctttatta attgctgcta atacagaagc aggtggtaac ggagcttgta cagatggtac
      301 cgaagttggt gtagaagtaa aaattggtgc aacaaatgat gcgaaatatg cttatgaatt
      361 agggagagtt tctggaattg aagcatcggc tattggatgt aactggagtt ttgctcctat
      421 tgtagatatt aatagaaatt ggagaaatcc tatcatttct agtcgatctt ttggacaaag
      481 ccctgataaa gttttagaac ttagtttagc ttatatgaaa ggtattcaag agagtggaat
      541 tttgcctgca gctaaacatt tccctggtga cggtattgac gaacgagatc aacatctatc
      601 ttttagtatt aatagtttaa gtac
//
LOCUS       DACECZ010000287          620 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.300, whole genome shotgun sequence.
ACCESSION   DACECZ010000287 DACECZ010000000
VERSION     DACECZ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.300"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            43..531
                     /locus_tag="IWO35_002853"
     CDS             43..531
                     /locus_tag="IWO35_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0317833.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
BASE COUNT          250 a           52 c          111 g          207 t
ORIGIN      
        1 tgagcaatag aattgtacta gtaggataag gatggatgga aaatgaaaaa aactattttt
       61 gttttagcga tttttatatg ttttgggcta cttttggtag gctacttgtt gtttaattct
      121 aatgaaatga gaactatttc tacgaatgac ttgttaaaaa aaagtgaatt aaaggataaa
      181 aaaaaagaat ctcttgcctt tataatgttt actcaagaag agtgtgagta ttgtatagca
      241 ataaatccaa tcatagatag tgtaaataag gaaactttga ataagataaa gaaatatgat
      301 atatctgtag attttcaatc taaacaaaag tgtaaaatta agtttgttcc gactattgtt
      361 gcatatagta agaataaaga agtggggaga attgacaatg aattagataa tttgtatcga
      421 aaaaagcaag atggttcaat tgtaatagac aagaaaaaaa caaagacatt gcttatagat
      481 tggatgaaaa aaatggataa acaaggagaa tatttgtatg gagaagatta aacagacatt
      541 tccgttaatt aataaatatt ggaataagaa aaaaattttt atatttttta ttacgtttct
      601 gctgtcatct ttattttctc
//
LOCUS       DACECZ010000288          618 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.301, whole genome shotgun sequence.
ACCESSION   DACECZ010000288 DACECZ010000000
VERSION     DACECZ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.301"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            374..>618
                     /locus_tag="IWO35_002854"
     CDS             374..>618
                     /locus_tag="IWO35_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317834.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIP"
BASE COUNT          243 a           76 c          115 g          184 t
ORIGIN      
        1 aggtgatcaa aatgggagta aacgaaatga tgaaagtcag attgagtttt taaatgatac
       61 cttcctcaga acttcaaaac gaattcaaag attcgttgac aaatcttgag caagatcatt
      121 taaaaaaata ttttctcttc atttgacgaa aaatgtttag aaatcttata taagtgaaga
      181 taaagtaatt ggtgtaatta attgtagaga atagcctttg agcaattgaa gcgatacaat
      241 tatttagaaa atgaacttcc tccagcatac tacatgattc cttacgggac gatgttaaag
      301 aagaaagcaa gaaattttag aagtattagt agataaacta atgggctttt ctcaatttaa
      361 gggagtagcc caaatgttta tcaaatatgg tgcagataca gtagcaacca tgtttacaga
      421 agaatatcaa gtagactatt atgaagtaat agataaatgt gtacattttg tttatgaaaa
      481 agtgtggaat tttatgaatt ttgcgataag tgaagaaatc ttacgagtac aagaaaacag
      541 aacaggaata tttaccagag acagaaagct taatttgatt gcgcattata tacggaatcg
      601 ctggtcatca ataccaaa
//
LOCUS       DACECZ010000289          592 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.302, whole genome shotgun sequence.
ACCESSION   DACECZ010000289 DACECZ010000000
VERSION     DACECZ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.302"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..333
                     /locus_tag="IWO35_002855"
     CDS             <1..333
                     /locus_tag="IWO35_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317835.1"
                     /translation="SFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYT
                     TTILTTVLENINEVSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHD
                     VLRYAYIY"
     gene            397..>592
                     /locus_tag="IWO35_002856"
     CDS             397..>592
                     /locus_tag="IWO35_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ0317836.1"
                     /translation="MILLILSLVLLVVSNLMKNNPPTKINSFYGFRTKKSMKNEKSWH
                     LAQKLLGDFSKKKVIPVFLMG"
BASE COUNT          223 a           71 c           91 g          207 t
ORIGIN      
        1 tcgtttacga gtgaatttta ctttttaaga cataaagaga gaattgtagg aatggtttct
       61 ataattgtta atccatttca aaacttatcc tacagtatag taaattacct catatgtgag
      121 cctgcatata ctacgactat tttgacaaca gtattagaaa atattaacga agtttcaatt
      181 ttagaagtaa agaagctagt gattcatttg ctaaattcaa cagaagatga atcactaatc
      241 ccctatttaa aagattcaca tttttttaaa gaagcggtac tatctaaaga atatagagag
      301 catgatgttt tgagatacgc ttacatttat taaataaaat ttctgatagt attttaataa
      361 ttcggatcat atcataagat ataggaggga aaagaagtga ttttattgat tttgtctctc
      421 gtattactag ttgtatctaa tctaatgaaa aataatcctc ctacaaaaat taattctttt
      481 tatggcttta gaactaaaaa gtcaatgaaa aatgaaaaaa gttggcattt agcacaaaaa
      541 ttgttggggg atttttcaaa aaaaaaagtt atcccagtat ttttgatggg ta
//
LOCUS       DACECZ010000290          581 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.303, whole genome shotgun sequence.
ACCESSION   DACECZ010000290 DACECZ010000000
VERSION     DACECZ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.303"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>581
                     /gene="eda"
                     /locus_tag="IWO35_002857"
     CDS             <1..>581
                     /gene="eda"
                     /locus_tag="IWO35_002857"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ0317837.1"
                     /translation="IAVVRGKNKDEAIKAINSIIAGGIKGIELTFTVPNAEEIIKEIA
                     KKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTAEICNLYQIPYLPGCM
                     TITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMPTGGVSLENLEDWFEV
                     GVVAVGVGGNLLASVEKGDFEEVSRVASKYIDK"
BASE COUNT          188 a           82 c          132 g          179 t
ORIGIN      
        1 cattgcggtt gtacgtggga aaaataagga tgaggccatt aaagcaataa attcaattat
       61 tgcgggtgga attaaaggga ttgaactaac ttttacagtt cctaatgctg aagaaataat
      121 caaagaaatt gctaaaaaat atgctgatca aaaagatgta ataatcggag ccggtacggt
      181 tttagatgcg tttactgctc gtatcgcaat tatggctggg gcagaatata tcgttagtcc
      241 atcttttgat cgacaaacag ctgagatttg taatttatat caaattcctt atttaccagg
      301 ttgtatgaca attactgaga taaaggaggc acttactgct ggcgtagata tcattaaact
      361 attcccaggt tctgcttttg gtccatccat tgtaaaagcc tatcaagctc cattaccaca
      421 cgtaaatata atgcctactg gcggagtaag tctagagaac ttagaagatt ggtttgaagt
      481 aggagttgtt gcagttggtg taggtgggaa tttattggct tctgttgaaa aaggtgattt
      541 tgaagaagta agtcgagtag ctagtaaata tatagataag t
//
LOCUS       DACECZ010000291          576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.304, whole genome shotgun sequence.
ACCESSION   DACECZ010000291 DACECZ010000000
VERSION     DACECZ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.304"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            95..565
                     /locus_tag="IWO35_002858"
     CDS             95..565
                     /locus_tag="IWO35_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ0317838.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          209 a          102 c           89 g          176 t
ORIGIN      
        1 ttattgtctg aaaagagttc taaacgggaa aacaaatagc tagttttcag tttagcgctc
       61 tttttattat ataaaataga aaggagaaaa ctttatgtct atttataatt ttgaaactac
      121 tttagaagac ggcactgtct attcattgaa aaaatatgca ggcaaaccga taatcattgt
      181 taacactgca acaaaatgcg gtctagcacc tcaatttaaa caattagaag aactttacca
      241 agcttatcag acacaaggtc tggttatctt aggctttcct tccaatcaat ttaaacaaga
      301 gctagattca gcgactgaag cagcagaagc ttgtaaaacc acttatggcg ttaccttccc
      361 tatgcatcaa ttgattgacg taaatggaga aaatacagat cctcttttta cttatcttaa
      421 aaatgaagct cctggtactg tcactaaagc aataaaatgg aattttacta aatttttgat
      481 tgatagaaat ggcaaagtaa tcgaacgatt tgcaccacaa acttctccat tgaaaatgac
      541 cgctgcaatt gaatccgttc tttaacttaa taaaaa
//
LOCUS       DACECZ010000292          536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.305, whole genome shotgun sequence.
ACCESSION   DACECZ010000292 DACECZ010000000
VERSION     DACECZ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.305"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(55..357)
                     /locus_tag="IWO35_002859"
     CDS             complement(55..357)
                     /locus_tag="IWO35_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317839.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          184 a           92 c           75 g          185 t
ORIGIN      
        1 ttataagtat ggtgataaaa tccgttccgg gctacctttt ttaggtagcc tactttaatc
       61 tttatacctt tctggatcaa cgaaagtata cttcaaatag tcataacgcc gatgatcgct
      121 ccgtgcgtct ggcacatcag tcacgatatc aaacaaaaaa tacacatctt tcttcattct
      181 ggtttttgtt gccggaattt taaagtagtt cttattagag taatagagat tgattaataa
      241 gctatcttcg attgctaaaa agaaaacttc tgaatcccac accttataaa aatctttgac
      301 aaatctattc gaaggatcaa atttaaacca taattgtgtt tttccttcca tcaacataat
      361 gttcacctca aaaagagtat acgaactaat gttctttttg taaagtagga atatcattga
      421 taatatggat ggaacatata tattaaaaag tgttcttttt gaatgctatt ttgaatgcca
      481 ttttcattca tttaacgaaa atctgcgaaa ataaaaaatg ctcaaaccct tataaa
//
LOCUS       DACECZ010000293          533 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.306, whole genome shotgun sequence.
ACCESSION   DACECZ010000293 DACECZ010000000
VERSION     DACECZ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.306"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(170..532)
                     /locus_tag="IWO35_002860"
     CDS             complement(170..532)
                     /locus_tag="IWO35_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317840.1"
                     /translation="MKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLFA
                     GYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVPQ
                     ILGSLWAVALSYKKARHP"
BASE COUNT          213 a           68 c          107 g          145 t
ORIGIN      
        1 tattattgga atcattattg ctgcaattat tacacaactg tttttagtgt ttggagagaa
       61 gaaaagaagt aaaaaataga aaaactagtg atagttttgg tgatataaga aaaacatata
      121 tgtataaaaa gaagaaagta gcttctcttt gtacatatat ggtttgtttt tatggatgcc
      181 gagctttttt ataagataag gctaccgccc ataacgaccc aaggatttgt ggaactaagt
      241 aaatacctgc aagaagcaag atcagctggg tcggttttaa aggatgatag aagtaacgcc
      301 attcagtaaa attggcgcaa aataaatagg aagtgacagt agatataata ttggcaatga
      361 ataaagatat aatacgcctg ttcctttgaa aataatatcc agcaaaaaga gataaaaata
      421 tcaatgtgat gaacccgctt acggaatgat aacgaaaatc aagcagtaag gcgaaatgca
      481 cataaggaac aaaagataaa agcaacggaa ggtttttcca gcaagttttt atc
//
LOCUS       DACECZ010000294          533 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.307, whole genome shotgun sequence.
ACCESSION   DACECZ010000294 DACECZ010000000
VERSION     DACECZ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.307"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(66..287)
                     /locus_tag="IWO35_002861"
     CDS             complement(66..287)
                     /locus_tag="IWO35_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0317841.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
BASE COUNT          184 a           88 c          102 g          159 t
ORIGIN      
        1 tttttttgtt ttcattaaga cgtattacct aagagttcca tgactttagt ctggtagcgt
       61 gtcagctaac caaaaaagag aaaaagaaaa gtaaaaaatt cgactttctt ttttctatag
      121 tatataaatt tttgcatata gataaagatc aagataccga taacggaagc tagattgtaa
      181 tctattttag tgacagtcag gtatagccaa gtaactagaa gatcagtgga acctgcacca
      241 taaaagtttg agtttgtcgg tccgttgaca gttaaaagaa aaatcacgtt gaagttattg
      301 atatttccga ttacgccgct tcgatttggt cacgatccaa attttggata atccctgtag
      361 aaattagcat agtagctggg ataccaattc acatattgac aaagataaca gagactttta
      421 cataaaacgg atcaatcagg aaaggaattg ctcgatccag ccccagtttt ccaacaatgt
      481 gttaactggc ctagcgtcat tcagcaaatt atgcatgatg aggagcgaaa tga
//
LOCUS       DACECZ010000295          532 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.308, whole genome shotgun sequence.
ACCESSION   DACECZ010000295 DACECZ010000000
VERSION     DACECZ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.308"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..277
                     /locus_tag="IWO35_002862"
     CDS             <1..277
                     /locus_tag="IWO35_002862"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAQ0317842.1"
                     /translation="YEKVQIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDK
                     IILMAYCELNENEVKDHRPKVVFVDDNNKVERVTSYEKHGRLSDIIL"
BASE COUNT          218 a           54 c          105 g          155 t
ORIGIN      
        1 atatgaaaaa gtacaaatag ttgatgttaa taatggaaat agatttgaaa cttatacaat
       61 agctggagag agaggaacag ggatgatttg cttgaatgga gctgcagcta gatgtgtatc
      121 aactggagat aaaataattt taatggcata ttgtgaatta aatgaaaatg aggttaagga
      181 tcatagacct aaggttgttt ttgtcgatga taataataaa gttgaaagag taacatcata
      241 tgaaaaacat ggaaggttat cagatataat tctttaggtt ttgtaatgtt atttatatat
      301 agctagacta ttattgaaat atttattgat tactaattgg atagatgtca ataaagtaga
      361 cacaaaaaga gaagatacag actgtagaca aagtccattt tatgtgaatc ggctacacta
      421 gactagacag aaaaaataag gtgtgtagaa taataaaaaa cacactggag gatttttcat
      481 gtcaagacgt caacgaagaa cctattcaaa agaattcaaa caacaaatcg tc
//
LOCUS       DACECZ010000296          523 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.309, whole genome shotgun sequence.
ACCESSION   DACECZ010000296 DACECZ010000000
VERSION     DACECZ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.309"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(134..433)
                     /locus_tag="IWO35_002863"
     CDS             complement(134..433)
                     /locus_tag="IWO35_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0317843.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          168 a          101 c           61 g          193 t
ORIGIN      
        1 aaaatacaat ataaagaaac atctgtttct ttatttagaa taaaaaaata cagactctgt
       61 tcaaataaat gaacctagcc tgcattatga gattgactat acttcttaat tcattgagat
      121 gaataactcg tttttataaa tgcccaaaag taatcggtgc cgaagccatt cctcgaccta
      181 attctttttc aaattttcct attgttgata tctccatata aaacggtgat actggtttag
      241 acaattctcc cctgattgct aacggacgta aactacgttc caaattattt atgactttag
      301 gataatactg acctttttct aagcctgtct ttgcgtctat caaaagtata cgttcctttt
      361 ctttgatctc tggatttaaa acgagatcat ataattttag taccatgttt tctatttctt
      421 cacttttaga catcacgatt tcctcccaaa aatatatttc ttcccactac attctataaa
      481 caaagtatac gctacatcta gattttttta aatcattact gga
//
LOCUS       DACECZ010000297          521 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.310, whole genome shotgun sequence.
ACCESSION   DACECZ010000297 DACECZ010000000
VERSION     DACECZ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.310"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>521
                     /locus_tag="IWO35_002864"
     CDS             <1..>521
                     /locus_tag="IWO35_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAQ0317844.1"
                     /translation="NMGSSRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTES
                     KIPLLIAANTEAGGNGACTDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWS
                     FAPIVDINRNWRNPIISSRSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDE
                     RDQHLSFSINSLGT"
BASE COUNT          170 a           70 c          117 g          164 t
ORIGIN      
        1 aatatgggat ctagtcgtga taaaaattac ttgagtaata tggtcaatca gtaccatatt
       61 ggagctgtac gatataatcc aggaactgct gatgaggtat atgatcaaaa ctatatctta
      121 caaacagaaa gtaaaattcc tctattaatt gctgctaata cggaagcagg tggtaacgga
      181 gcttgtacag atggtactga agttggtgta gaagtaaaaa ttggtgcaac aaatgatgcg
      241 aaatatgctt atgaattagg gagagtttct gggattgaag catcggctat tggatgtaac
      301 tggagttttg ctcctattgt agatattaat agaaattgga gaaatcctat catttctagt
      361 cgatcttttg gacaaaaccc tgataaagtt ttagaactta gtttagctta tatgaaaggt
      421 attcaggaga gtggaatttt gcctgcagct aaacatttcc ctggtgacgg tattgacgaa
      481 cgagatcagc atctatcttt tagtattaat agtttaggta c
//
LOCUS       DACECZ010000298          517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.311, whole genome shotgun sequence.
ACCESSION   DACECZ010000298 DACECZ010000000
VERSION     DACECZ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.311"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          225 a           53 c           82 g          157 t
ORIGIN      
        1 tatatccaac aaagaataag gtacaaaata caagcaagtg agaaattcag ggatgtcatt
       61 ataaaaaaac tagcgaacaa ttaaatagaa taacatccgt taattaaatg taagtatctt
      121 ttagatgaac gaaaaaacaa taaaatgctg taatattcgt gtttaaaaat aataaataaa
      181 aatagggaat tttctattgc aattttataa agagattgct atattattat ttgttctaaa
      241 tgattccttt aaggtgtcac taaaaattat aaaaaatagt gttgacgcac ttttgatgtc
      301 atgttataat aacgaagtcg tttggatgag atagctaata aagcttgatt ttatcgaaaa
      361 aaacttcgaa aaagttattg acaaataaca ataaagatgg tatgataatc aagtcgctgc
      421 taatgagtgg caaagacgtt tagaaaaaat aaacaaagaa gttgttgaca ttctcttctg
      481 aatctgataa gattaaagag ttgttaaaac aaaaaga
//
LOCUS       DACECZ010000299          504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.312, whole genome shotgun sequence.
ACCESSION   DACECZ010000299 DACECZ010000000
VERSION     DACECZ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.312"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>504)
                     /locus_tag="IWO35_002865"
     CDS             complement(<1..>504)
                     /locus_tag="IWO35_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0317845.1"
                     /translation="GLSSDFSENMVFVDYSTSQEILNNSENNEIANKILMYSGSAEST
                     DLALNKLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVMTYSIMLGGMVVLSLI
                     LILWLRERIYEIGIFLSIGTSKIQIIMQFIFELIFISIPSIISSLFLGNVLLKVIVDG
                     FINSEDS"
BASE COUNT          182 a           86 c           48 g          188 t
ORIGIN      
        1 ttgaatcctc tgagttaatg aatccatcta caattacttt tagtaataca tttcctaaaa
       61 ataaggaaga tattatactt ggtatcgata tgaatattaa ctcgaatata aattgcatta
      121 taatttgtat cttagatgtt ccaatagata agaatatacc tatttcataa attctttccc
      181 ttaaccatag aatcaagatt aatgaaagaa caaccatccc acctaacata atagaataag
      241 tcattacttt tattatatat tttattccac tcactgactc taaagactct tcaaacgcat
      301 tagaatcttt ttcaacaaaa tactttgact catcaatttt aagctctttc aatttgttta
      361 aggctaagtc tgtagattct gcactaccag aatacattaa aattttattt gcaatttcat
      421 tattttctga attatttaat atctcttggc ttgttgaata atctacaaac accatatttt
      481 cactaaaatc agaagataat cctg
//
LOCUS       DACECZ010000300          496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.313, whole genome shotgun sequence.
ACCESSION   DACECZ010000300 DACECZ010000000
VERSION     DACECZ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.313"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..387
                     /locus_tag="IWO35_002866"
     CDS             <1..387
                     /locus_tag="IWO35_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0317846.1"
                     /translation="RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGK
                     CAITLEEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIAL
                     LKLNKVQLIRLNKYRVLAGNQELNLI"
BASE COUNT          193 a           78 c           89 g          136 t
ORIGIN      
        1 agaaaattta tccacaccaa tctaagtgtt gataaattac tgatgtacca gttaatgaaa
       61 ttatctttgg aaaatcgcac aatagaatac gcagacaatc gaatttcgct attttctgct
      121 caacatggaa aatgtgctat aactctggaa gaatttcaac aagctaatga aatacattgt
      181 catcatattg taccaactgg agctggtgga aatgacgatt ataaaaatct tattttggta
      241 aaagaggcgg tacaccgtct aatacacgct aaaacagaag aaacaataca aaaatatata
      301 gctctattga agctaaataa agtccagctc ataagactaa ataaatatag agtattagct
      361 ggcaatcaag aattaaactt gatataggtc tactacataa atacttttca atataaacac
      421 gatggaacgc ggagtgcggt gaaagtcgca cgctccgtgt gaatgggggg aaaagtttga
      481 gatatttata atatca
//
LOCUS       DACECZ010000301          491 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.314, whole genome shotgun sequence.
ACCESSION   DACECZ010000301 DACECZ010000000
VERSION     DACECZ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.314"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          185 a           75 c           79 g          152 t
ORIGIN      
        1 ttttttgtca tctgtatcta taatacaaag ccaagaacgt tattcaaaat atggatagct
       61 atgataaaag ctaatgagcg attcgcagtt aaataagcaa tcgtcagtaa aatcgttcca
      121 ggagcgtaca tgaaaaaatc aattggatgc gttacatgaa taaagataat tctcttttat
      181 ttgtacgaat aaataccaaa acgtacctcc taaaattaga ttttggacag atgcccttta
      241 aaaagataac aagatttcgt tcttgaaaca atatatatgc ttttattcca atgaggttgt
      301 gaaaacagta ttttgtcctg agcgttagaa aattgaaaat ggggaatagc aactcgtact
      361 ttaccatttt ttaagtgaat acaagatcag ctaattgaac accgtttatt cgtgatgagg
      421 gacacaacac tgcagtcgac acatttctct tttatgttca aagataacaa atatttaaaa
      481 caaagaaaaa t
//
LOCUS       DACECZ010000302          481 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.315, whole genome shotgun sequence.
ACCESSION   DACECZ010000302 DACECZ010000000
VERSION     DACECZ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.315"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(58..255)
                     /locus_tag="IWO35_002867"
     CDS             complement(58..255)
                     /locus_tag="IWO35_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0317847.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          156 a           89 c           67 g          169 t
ORIGIN      
        1 tttttctacc gacataaaca aaaaaaccac ctcatatgag gcggttaacg ttcaacatta
       61 ttttacgata tttactgctt gaggaccacg ttgtccttct tcaatatcat aagtaactgc
      121 ttggccttca tctaaagact taaagccttc tccttggatt gctgagaaat gagcgaatac
      181 gtcatttcca tcttcaccag tgatgaaacc aaaaccttta tctgagttaa accattttac
      241 tgtaccgtta ttcatatata tttcctcctg atacgtatat aatacgtgtt ttattgcaat
      301 taatattact tggtaaaagg agtttttata gtgtgaatat atcgctcaga ttacgtttca
      361 aattagatta cttatttact ataacacgga tgtaatgcta acacaaggat aatcactcga
      421 tatacggttt aaatattatt atttgtcaaa ttaagcgaga caaaactttc catttacgta
      481 a
//
LOCUS       DACECZ010000303          480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.316, whole genome shotgun sequence.
ACCESSION   DACECZ010000303 DACECZ010000000
VERSION     DACECZ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.316"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>480
                     /locus_tag="IWO35_002868"
     CDS             <1..>480
                     /locus_tag="IWO35_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAQ0317848.1"
                     /translation="HFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTT
                     IYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYL
                     "
BASE COUNT          166 a           80 c           95 g          139 t
ORIGIN      
        1 cattttaaag gaaagcaatt tcagcaggat gtgattattg tagccgtggg ctactatctt
       61 cgttataacc ttagctatcg tgaagttcaa gaaatcttat atgatcgtgg cattaacgtt
      121 tctcatacga cgatttatcg ttgggtgcaa gaatatggca aactactcta tcaaatttgg
      181 aaaaagaaaa ataaaaaatc cttttattca tggaaaatgg atgaaacgta catcaaaatt
      241 aaaggaaaat ggcattattt gtatcgagcc atcgatgcag atggtttaac cttggatatt
      301 tggttacgta aaaaacggga cacacaagca gcctatgctt ttcttaagcg gttagtgaag
      361 cagtttgatg aaccgaaggt tgtagtcaca gataaagccc cctctattac aagtgccttt
      421 aagaaactaa aagaatacgg cttttatcaa gggacagaac atcgtaccat taaatacctg
//
LOCUS       DACECZ010000304          449 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.317, whole genome shotgun sequence.
ACCESSION   DACECZ010000304 DACECZ010000000
VERSION     DACECZ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.317"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>449
                     /locus_tag="IWO35_002869"
     rRNA            <1..>449
                     /locus_tag="IWO35_002869"
                     /product="23S ribosomal RNA"
BASE COUNT          136 a           99 c          132 g           82 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggca
//
LOCUS       DACECZ010000305          447 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.318, whole genome shotgun sequence.
ACCESSION   DACECZ010000305 DACECZ010000000
VERSION     DACECZ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.318"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(15..86)
                     /locus_tag="IWO35_002870"
     tRNA            complement(15..86)
                     /locus_tag="IWO35_002870"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52..54),aa:Gln,seq:ttg)
     gene            complement(96..168)
                     /locus_tag="IWO35_002871"
     tRNA            complement(96..168)
                     /locus_tag="IWO35_002871"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(133..135),aa:His,seq:gtg)
     gene            complement(230..314)
                     /locus_tag="IWO35_002872"
     tRNA            complement(230..314)
                     /locus_tag="IWO35_002872"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(278..280),aa:Leu,seq:caa)
     gene            complement(335..405)
                     /locus_tag="IWO35_002873"
     tRNA            complement(335..405)
                     /locus_tag="IWO35_002873"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(371..373),aa:Cys,seq:gca)
BASE COUNT          134 a          107 c           98 g          108 t
ORIGIN      
        1 tttaaaaata ataactgggg tagctggatt cgaaccaacg catgagggag tcaaagtccc
       61 ttgccttacc gcttggctat accccaagaa taaagggcga atgatgggaa tcgaacccac
      121 gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc acattcgcca tcataaaaga
      181 tgttgtttaa tttctcaata aaaaactaag atgaagacat cttagtcaat accggcggcc
      241 ggggtcgaac cggcacgccc tcaacgggca ctggattttg agtccagcgc gtctgccaat
      301 tccgccacgc cggcaagttg atttattagt actaaggcgg taaccggatt tgaaccggtg
      361 atgaaggttt tgcagacctc tgccttacca cttggctata ccgccattat actattatca
      421 atctataaaa taaagaataa attatag
//
LOCUS       DACECZ010000306          440 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.319, whole genome shotgun sequence.
ACCESSION   DACECZ010000306 DACECZ010000000
VERSION     DACECZ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.319"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>440
                     /locus_tag="IWO35_002874"
     CDS             <1..>440
                     /locus_tag="IWO35_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0317849.1"
                     /translation="NIELLMETTDNSSYPKPISDYINYLNNMKNRYEQKNVNNIASYI
                     RDEIIMGKYEIDEKDNSVSFRQKFGKNRYRKERIPFHVVSSSIKSLYGLDYYLENSSK
                     EGDYLIIDEPELSLHPENQVKLAKVITDIVESKIKLITSTHSDL"
BASE COUNT          179 a           46 c           73 g          142 t
ORIGIN      
        1 aatattgagc tgttaatgga gacaacggat aatagttctt atccaaagcc aatatcggat
       61 tatattaatt atctgaataa catgaaaaat agatatgaac agaaaaatgt caataatata
      121 gcatcatata ttagagatga gattattatg ggtaaatatg aaattgacga aaaagataat
      181 tctgtaagtt ttagacaaaa gtttggtaaa aatagatata gaaaagaacg aattcctttt
      241 cacgttgtgt cttcttcgat taaatctcta tatggattag attattactt agaaaattca
      301 agtaaagaag gagactatct tattattgat gagccagaat tgagtttaca tccagaaaat
      361 caggttaagt tagcaaaagt aattacagat atagttgaga gtaaaattaa gctaattact
      421 tctacccata gtgatttatt
//
LOCUS       DACECZ010000307          426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.320, whole genome shotgun sequence.
ACCESSION   DACECZ010000307 DACECZ010000000
VERSION     DACECZ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.320"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>426
                     /locus_tag="IWO35_002875"
     CDS             <1..>426
                     /locus_tag="IWO35_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0317850.1"
                     /translation="VIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFIGE
                     PNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLPEH
                     ELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKV"
BASE COUNT          175 a           40 c           75 g          136 t
ORIGIN      
        1 gtaattattg aatataacaa tgttcctatt ggatatggac aaatatataa aatgtatgat
       61 gagttatata ctgattatca ttatccaaaa actgatgaga tagtctatgg tatggatcaa
      121 tttataggag agccaaatta ttggagtaaa ggaattggta caagatatat taaattgatt
      181 tttgaatttt tgaaaaaaga aagaaatgct aatgcagtta ttttagaccc tcataaaaat
      241 aatccaagag caataagggc ataccaaaaa tctggtttta gaattattga agatttgcca
      301 gaacatgaat tacacgaggg caaaaaagaa gattgttatt taatggaata tagatatgat
      361 gataatgcca caaatgttaa ggcaatgaaa tatttaattg agcattactt tgataatttc
      421 aaagta
//
LOCUS       DACECZ010000308          421 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.321, whole genome shotgun sequence.
ACCESSION   DACECZ010000308 DACECZ010000000
VERSION     DACECZ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.321"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          139 a           78 c           49 g          155 t
ORIGIN      
        1 gaaagcacaa aaaataaaaa aattattacg ttattaaaaa aacagatgaa agatcaggtt
       61 ttattagatc tcattctttc atcacttgaa gggatttaat gaactacttt ttcctctttg
      121 tcgtttttat atattcgtat tccttcaaac agtaggatag cctctttcaa tgcttgatat
      181 ttgaactgtc cgttacttaa aaatttagat gtgccgccct cttcctagaa catattcttt
      241 tttacttttt cattaattga cagaccaatc gaacgaaact gtaaatttcg ataggataat
      301 gattggtcaa actcattcat tttcaagaca tctcgattca tattcaagat aggaatcttt
      361 ttatcaacat cactttattc atattttcct ctaccataaa agcttcacgc tccacaaaat
      421 t
//
LOCUS       DACECZ010000309          401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.323, whole genome shotgun sequence.
ACCESSION   DACECZ010000309 DACECZ010000000
VERSION     DACECZ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.323"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          116 a           71 c           54 g          160 t
ORIGIN      
        1 gttatagttt aagaaagttt aaacattgcg caatttaatt attttagctg tttcttttca
       61 taaaaaaaca tgttgtgact ctgagacaaa tcatagtcca tagtgatgtc tctagagtaa
      121 ctacgcctaa tattacgagg catcatatta atctatcagc actagtacct aaactagcat
      181 ccctttcgaa tattttttct tatataataa agtttgcttt cactcctcta atctcaccaa
      241 atcgactggc ttgcgttgtg tgatactagg aactgattgg tttagaacga cttttctatt
      301 gtaatcatat ttttcgccat cctcgttgtc ttctaatcct tcaatgaatg atccctaatg
      361 tgtgtattaa ataatttaat ttttttgttc ctatttattt a
//
LOCUS       DACECZ010000310          387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.326, whole genome shotgun sequence.
ACCESSION   DACECZ010000310 DACECZ010000000
VERSION     DACECZ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.326"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          141 a           50 c           57 g          139 t
ORIGIN      
        1 attatcaagc cattattcct tcttctacta gttctttttg tcacataatt ttgcaatcaa
       61 taaatgttgc gcaataagta taagaattcc attttctaaa tttgaaaggt gatccataat
      121 ttcatgttca agtagtttat taattgtcca cttgtcgctt catctaaaaa ttttataggt
      181 tttaggaatg ctattcgctt attttaggaa gattttaact gtttgctata gatatatatc
      241 atactagtat cgtaataaaa gtactctaaa agaagaattt tcaaatacgc aaaattaagt
      301 acgaaagagt tgaaacaaat ttggagaaaa tgagcataga atgtctgatg agttaaatag
      361 acctaaaaat tatctaaaag tggagca
//
LOCUS       DACECZ010000311          365 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.329, whole genome shotgun sequence.
ACCESSION   DACECZ010000311 DACECZ010000000
VERSION     DACECZ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.329"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>365
                     /locus_tag="IWO35_002876"
     CDS             <1..>365
                     /locus_tag="IWO35_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0317851.1"
                     /translation="FNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFHTDRGKEF
                     DNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELF
                     DYVNWWNHLRLHGTLGYET"
BASE COUNT          117 a           64 c           71 g          113 t
ORIGIN      
        1 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
       61 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      121 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      181 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      241 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      301 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      361 gacac
//
LOCUS       DACECZ010000312          364 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.330, whole genome shotgun sequence.
ACCESSION   DACECZ010000312 DACECZ010000000
VERSION     DACECZ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.330"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>364
                     /locus_tag="IWO35_002877"
     CDS             <1..>364
                     /locus_tag="IWO35_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HAQ0317852.1"
                     /translation="RWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRA
                     IDADGLTLDIWLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGL
                     YTKTEHRTVKYLNNLIEQ"
BASE COUNT          123 a           67 c           77 g           97 t
ORIGIN      
        1 atcgttgggt gcaagagtac agcaaagtcc tctattatct ttggaagaag aaaaatagac
       61 aatccttcta ttcatggaaa atggacgaaa cctatatcaa aattaaggga cgttggcatt
      121 atctttatcg tgcaattgat gcggacggct taaccttaga tatctggtta cgaaagaaac
      181 gggatacgca agcagcctat gctttcttaa aacgactcca taaacagttt ggtgagccga
      241 aagcaattgt gaccgataaa gcaccttctc ttggctccgc ctttagaaag ttacagagtg
      301 tgggtttata tactaagaca gagcaccgaa ctgtgaagta tcttaacaat ttaatagaac
      361 aaga
//
LOCUS       DACECZ010000313          364 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.331, whole genome shotgun sequence.
ACCESSION   DACECZ010000313 DACECZ010000000
VERSION     DACECZ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.331"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          104 a           67 c           55 g          138 t
ORIGIN      
        1 cttctagatt agaaattgat ttttgaattt tttgtgtctc ctgtaaagtt cgctgaactt
       61 gtaaagctga ttcttctttc ggtaaattaa ttttacttag aatatcgcta tcttgataaa
      121 aatttggtgt gttcacaaat tctggcataa aaaatcccta ctttctagtt aaggttcctg
      181 aataagtcgc aatccaattg tttccagctg atttataact ttgtaaatac aattttccat
      241 agtaccaata accatttctt ttttctttcc aattccgtga ttgaggaggt ctagaaggcc
      301 aaccaacttt atcaaaaatc atcgttcctg tcactcgtat cgttttagaa gctgctcgag
      361 caag
//
LOCUS       DACECZ010000314          364 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.332, whole genome shotgun sequence.
ACCESSION   DACECZ010000314 DACECZ010000000
VERSION     DACECZ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.332"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>364
                     /locus_tag="IWO35_002878"
     CDS             <1..>364
                     /locus_tag="IWO35_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAQ0317853.1"
                     /translation="EDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFTKEMTH
                     DEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGEREK
                     QFAYSAHTSCDDNGFILST"
BASE COUNT          137 a           62 c           76 g           89 t
ORIGIN      
        1 tgaggatatt tttgcctata ttttagaaca agcagttaaa gcaggttttg taacagagga
       61 taatttgtat ctggattcta cccacatcaa agctaacgcc aataaacata agtttacgaa
      121 agaaatgacc catgatgaag cgaaagccta tcaggatgaa ttagaagatg agatcaatcg
      181 tcaacgaata gaggctggaa aacgcccttt tactttggac ttagaaaagg aagtgaagct
      241 gaaagaaaga aaaataagta aagctgatcc ggaaagcggc tactacgtca aaggcgaacg
      301 agaaaaacaa tttgcttatt ctgcccatac ttcatgcgac gacaatggat ttattcttag
      361 caca
//
LOCUS       DACECZ010000315          357 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.333, whole genome shotgun sequence.
ACCESSION   DACECZ010000315 DACECZ010000000
VERSION     DACECZ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.333"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>357
                     /locus_tag="IWO35_002879"
     CDS             <1..>357
                     /locus_tag="IWO35_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317854.1"
                     /translation="AKWPEEATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTE
                     LNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVT
                     VTDNRSDADKYEPTVEG"
BASE COUNT          146 a           53 c           82 g           76 t
ORIGIN      
        1 tgcgaaatgg ccagaggaag caactcaacc aattacagca gatgatacag aatttgtatc
       61 aagtgcaaca aaactggatg ataaatctga cgctgacaaa tataatcctg aaggtcaaaa
      121 agtgactaca gaattgaata aggaaccaga cgcatttgag ggaattaaga acaaagaaga
      181 tttaccaaaa gatactaagt atacttggaa agaaaaagta gatgttagcg cagctggaaa
      241 taaaaaaggg actgttgtag tgacatatcc agatggatca tctgatgaag ttgaagtaga
      301 tgttacagtg acagacaatc gttctgacgc cgataaatat gagccaacag tagaagg
//
LOCUS       DACECZ010000316          355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.denovo.334, whole genome shotgun sequence.
ACCESSION   DACECZ010000316 DACECZ010000000
VERSION     DACECZ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.denovo.334"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          113 a           47 c           65 g          130 t
ORIGIN      
        1 ataacgtgat tttttgctgt aatgacaagg ttttagctgt ttcagatacc atgtatccaa
       61 aaatagttta ttgagaaaat aggagagtat ttgatactaa ctagtaggaa aaaatgagat
      121 aaaaagtaaa tggaatcatt taaggtaatc tgaaataaaa aatatacagc cctgtggtac
      181 tttacctaca aggctgtata tattttttac tctcagcgtt tttgactccg tgtctgactc
      241 cgttttattg attttgaatg ttattcaatg accatttaac aggtcaaatt tgatgttttt
      301 gattccgtat gacgccgttt gatatctagt gatatagtga tttgagtcta catcg
//
LOCUS       DACECZ010000317         8655 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.guided.102, whole genome shotgun sequence.
ACCESSION   DACECZ010000317 DACECZ010000000
VERSION     DACECZ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.guided.102"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..177)
                     /locus_tag="IWO35_002880"
     CDS             complement(<1..177)
                     /locus_tag="IWO35_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0317855.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTD"
     gene            305..874
                     /locus_tag="IWO35_002881"
     CDS             305..874
                     /locus_tag="IWO35_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ0317856.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            941..1678
                     /locus_tag="IWO35_002882"
     CDS             941..1678
                     /locus_tag="IWO35_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0317857.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            1909..3177
                     /locus_tag="IWO35_002883"
     CDS             1909..3177
                     /locus_tag="IWO35_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ0317858.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDCLTPMGWRYEGIGFYGY"
     gene            3664..4128
                     /locus_tag="IWO35_002884"
     CDS             3664..4128
                     /locus_tag="IWO35_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0317859.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            4112..4741
                     /locus_tag="IWO35_002885"
     CDS             4112..4741
                     /locus_tag="IWO35_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0317860.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            5095..6573
                     /gene="msr"
                     /locus_tag="IWO35_002886"
     CDS             5095..6573
                     /gene="msr"
                     /locus_tag="IWO35_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0317861.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            6620..7066
                     /locus_tag="IWO35_002887"
     CDS             6620..7066
                     /locus_tag="IWO35_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0317862.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(7265..7780)
                     /locus_tag="IWO35_002888"
     CDS             complement(7265..7780)
                     /locus_tag="IWO35_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0317863.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            7897..>8655
                     /locus_tag="IWO35_002889"
     CDS             7897..>8655
                     /locus_tag="IWO35_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0317864.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVG"
BASE COUNT         2816 a         1514 c         1794 g         2531 t
ORIGIN      
        1 atccgtcaac tgtatctctc gggagcctcg agtgaaaagt gtcacaccaa gttcttcttc
       61 taaatctttc agttgttttg agagagtagg ttgcgtgata tgcagagatt ttgctgctcg
      121 gctgatattt ttttctctag ctactgctaa aaaatacgtc agtatacgaa tatccatttt
      181 ttccctcctg tttttaaaaa agcataccac taagtatagt taaaaggaat agttaagtat
      241 gttttataag tattagacat cataagccgt ccgttataaa gtaaggataa aaggagggag
      301 caaaatgaca agcctcatcg aaaaacaaat cgtcaataaa gagattcgag taacggatcc
      361 gctctttgaa gtgatccatc agatccaagc ggaaaacgaa gagcctttag cagcgctgaa
      421 tactgggtac catgaaccag cagatatccg caaacggcta gaaaatatca ttagtgacaa
      481 agtagatgat acggtgactg ttctgctgcc tttttataca gatttcggca aacatattag
      541 tatagggaaa aatgtcttta tcaatcgtca agtgatgttc gttgacttag gtggcatctg
      601 tttagaagat tcagtattga ttggtcctcg tgtcaacctc atcacggtga accatctaac
      661 cgatccaaaa gaacgaagag gcttgtcagt aaagccaatc cacatcaaga aaaatgcttg
      721 gatcggagca ggagcaacta ttctgccagg cgtaacgatt ggcgagaatg ccattgttgc
      781 agcgaatgca actgttacga aagatgtccc agacaatacc atcgttgcag gaatccctgc
      841 caaaatcgtc aaacctgtcg aacggattat ttgaacagta agatgaaata agagatagtg
      901 agtataagca agagattttt caaaacagga ggagaataga atgaatgatt tatcagaaaa
      961 agtgattgtc atcatgggcg cttcaagcgg aatcggtgaa gcaacaacga agctattagc
     1021 agaaaaaggg gctaaactag ttatagctgc acgccgagaa gatcgcctaa aagcaataaa
     1081 agaatcttta ccagaagctg agctatatat tcaaacagca gatgtaagag attttgcaca
     1141 agtccaagca gttatcgatt tagcgatgga aaagttcggt cgaatcgatg tattgtacaa
     1201 taatgcagga atcatgccaa ccgctccatt agttgaaggt caccgtgatg aatggcaaaa
     1261 tatgcttgat atcaacatca tgggtgtgtt aaatggaatt tccgccgtac ttccaatcat
     1321 ggaaaaacaa aaatctgggc atatcatttc aactgattct gtggcgggac atgttgttta
     1381 tcctgattcc gccgtttatt gcggaacaaa atttgctgtc cgtgcaatca tggaaggatt
     1441 acgtcaagag caacggcaaa ataacatcaa atctacgatc atctctccag gagctgttca
     1501 aactgaatta tatcaaacca tcagcgacaa aaaggctgct cttgaacttc atgaagcgca
     1561 aaaagaatgg ggattaacat ctgaagatat agcttctgct gtagcatttg cgatagaaac
     1621 gccagaccgg atgtctgtca gcaacatgat tatccgtccg acgacacagg aagtataatc
     1681 cataacggat aatatccaga gagaattact tgttaaatag ttattcaaac gaccatcaat
     1741 ttaaggcgtg aatggagaag aatattcttc atttacgtct ttttatgcag aaaaagcaaa
     1801 tttattaaaa agaaacaaat gttatttaaa tatggacatt gacttgcttt tactttttta
     1861 gttacactgt tatttaggaa aagtttcata attcaaagga gaaaaaagat gaaagcaaag
     1921 aatttattat ttagcgttat tcttttagga aatctagcag ttgccaacac aacagtgcta
     1981 gctgaagaat cgacagaaac tgtcgcaact cagacatcga gttcttcaga aacaggaatt
     2041 gaaaacgata cagaaagtac tttgcagtca gctactgtct cagaaaatac aatttcaacc
     2101 gaagaaccaa caactgaaac aacagaacaa acacaaaccg aagacagtac atcagcagaa
     2161 gctcagaaca atccagagaa tgcagaagaa atcacgaacc aattgacaga tgggcaaaca
     2221 ttgacacata cccatcaagc agatttgtct gaattcgaaa actatactgg ctatgctttt
     2281 tatgctcgct catctgctaa ttctcaacaa gcgttcattg atagtattgc ctcaactgca
     2341 caaagtttgg caagtgccaa tgacttatat gcatcagtca tgattgctca agccatcgtt
     2401 gaaagtggct gggggaacag tactttggct tctgccccta attacaatct atttgggatt
     2461 aaaggaagct ataatggcca atctgttacg atgcctactt cggaatatgt gaatgggcaa
     2521 tggattacgg tgaatgccgc ttttagaaaa tatccttcgt acaaagaatc attgcaagat
     2581 aacgtaacgg tattgaagac gacttctttt cagccaggcg tttactacta ttccggtgca
     2641 tggaaaagca atacgaactc ttacaaagat gctacggctt ggctaacagg tcgttatgcc
     2701 actgcgccta attatggttc tacattgaac aatgtaatcg aaacctataa tctgacacag
     2761 tatgatacag caccaacttc aacttctccg atgtatcgtc tgtataaccg tcatacagga
     2821 gaacatttat atacattgaa tgccggagaa aaaaattatc tgccaactgt aggctgggaa
     2881 tatgaaggga ttgcttggca agcaccaaac agcggacaac ctgtctaccg tttatacaat
     2941 ccttacagtg gagaccatca ttacacaatg gcacaatctg agatcaactc tttaacaaaa
     3001 atcggttggc gttatgaagg tctaagtttt tattctggtg gttccaagcc aatttatcga
     3061 ctatttaatc caaatgaaaa aactggtacc catcactata ccttgagtgc taaggaaaga
     3121 gattgtctaa caccgatggg ctggagatat gaaggaatag gattttatgg ttattaaaaa
     3181 gaggtagtga aaaagctgcc ctgcatcaag tagtaagaac agccaaacaa aaacagattt
     3241 tcatgttttt tgtttggttg aacttattac cgcagatgca agtttttgaa caccgtttac
     3301 tcaaaaaagg ttgtgacaac atcttgtcat aactttttta ttactgttta agcatactga
     3361 tccaatttct aatattcgac gggattcttg caaaaggcga tttcggaaat ttttggcggt
     3421 gtaaaattat ctactgcaga agagctatct tctatagttt gataagaatc aataatcttg
     3481 gttttagcgt tgtgctttct ttatcatttt ttccgttcat tctacaaaaa atttcttttc
     3541 tctatacttt tctattgaga gcgttttttt tattacacag ctgattcttt gacttttttc
     3601 tttttttatt ctattattga caagtcaatc gttgacgagt caattattag aaaggaggaa
     3661 attatgtctt tagaagagat tagtcacctt ttatatcaaa tcaaattagc cgatcaaaaa
     3721 atcgtccaga tctttgaaga aaatatcggt ataagtctta ctagatacga gatcatgatg
     3781 agactgaaag aagaaaatcc attgctacaa agcagtttgc aagaaaagtt gcagattgat
     3841 caaggagcag ttactcgtca tctgaaggta ttggaagcaa atggctatgt ttcgcgtcag
     3901 agaaatcctc agaacaaccg ggaagtgtat gtagcgctaa cagaaaaagc ccagaagaaa
     3961 atcaagcatt gtgaaatgga ccataaagat acaggttccg tgctatcccc ggctttcagc
     4021 gatgtagaaa tgaaacaatt tctaacactt ttagataaat tgaataccca actttgcaat
     4081 ataacaacag aaacgaaaga ggttaataat aatggaacaa aaaaatgatt tttcaacaat
     4141 tatgactggt agaagatccg ttcgcgtata tgatgaaaca tacaaaatcc cacacgaaga
     4201 aatgctggaa atgatcaaag aagcgacgac tgcaccttct tctgtcaata tgaaaccatg
     4261 gcgctttgtg gtcgctgaaa gcgaggaagc aaaagaaatc ttgcgtcctt tgatccgttt
     4321 caatacgaga caaaatgata cttcttctgc aatggtcatg atttttggcg atatgcaatg
     4381 ttacgaatat ggcgaagaaa tctacaacca agcttacgaa tcagggaaga tgccaaaaga
     4441 agtcagagat caacaattag ctgcgatcat tccttattac aagagttttt caagagaaga
     4501 aatgaacgat gtagtcaaag tcgattctag cttagcggct atgcaattca tgttagttgc
     4561 gcgtgatcat ggctatgaaa caaatccaat tggtggattc gaagcagatc agttggcaga
     4621 ggcatttggt ttggataaag accgttatgt tccagttatt attttatcaa taggaaaagc
     4681 ggtagaagaa ggttacgagt ctgttcgttt agcaccagaa aagatcacta tgttcgaata
     4741 aaaggttttc agttactatt cagtagctat tgcttttttt gaatcgtgtg tggtaaccta
     4801 ataaaaaagg aggtcggaaa aatgactgca tcgatgaaat tacgtttcga acttttgaat
     4861 aacaactaaa taaattcaaa agacatgtat aaacaacaat ttcatggatg gagtatgtcc
     4921 attttttcga acgacagtag aataggtgaa agctatgaca gatttggtga tttagtcata
     4981 gttatttcgt tcagagtagg aaaagatgtg atgcagtttt tttgcaaaca gatttttctg
     5041 ctctttttta gtatcgataa ttgttgtttt acatgaaata ggagtgtaaa acatatggaa
     5101 aatttagcag taaatataac aaatctacaa gtcagctttg gcaaccagct agaactatct
     5161 attgattctc ttcgtgtcta tcagcaagat cgtataggga tcataggaga aaatggtgtc
     5221 ggtaaatcga ctttgctcaa actaatagcc ggtgaacttt ttcccgatca tgggaaaatc
     5281 caaacagaga tcaccttcaa ctacctgcct caattaacct atctcgctga agcaaaggac
     5341 ctaaatttgg aattagccag tcatttccag ttaagactgg aagaaacttc agagcggaaa
     5401 tggagcggag gggaagaacg aaagatcgag ttgatacgtc ttctttcttc ttatgaacaa
     5461 gggatgcttc tagacgagcc aacaacccat ctagatagaa aaagtattga tcgactgatt
     5521 gaagagcttc gttattatta tggtacgctg gtttttgtta gtcatgatcg ctattttcta
     5581 gatgagctag catcgaaaat ctgggaagta aaagacggag aaatccgaga gttttcgggg
     5641 aattatagtg cctatctcac tcaaaaggaa ttggagaaaa agactcagct acgagaagca
     5701 gagtcgatca tgaaagagaa aaaacgattg gaaaaatcga tccaagaaaa gaaaaaacaa
     5761 gcggaaaagt tggaaaaagt gtccagtaaa aagaaaaagc aacaaatcag actggatcgg
     5821 ttgtcttcct ctaaacaaaa agacagtgta caaaaagcca tccaaaagaa tgcgaaaaca
     5881 ttagagagaa gactccaaaa aataggagaa acaaccaagc cgcagcagat gaaacaaatc
     5941 cgttttccag taccaaaatc tcttgaactc cacagccgct atcccatcat gggacaaaat
     6001 gtccaattgg aaagaagtgg gagaacatta ctggtaaatg gtgattttca gttttcttta
     6061 ggtaaaaaaa tcgcgattgt cggtgaaaat ggttcaggta agacaacctt attagaacat
     6121 atccgcaaac aaggagaagg aatccttctc tctccgaaag taagctttca agtatatcag
     6181 caaaagggtt atcaaatgac atctgaagaa tccatcattc gttttgtcat gagacaaaca
     6241 gagttttcag aatcgcttgt ccgtagtttg ctgaatcact tagggtttgc tcaggaaact
     6301 ttgacgaaac cgttatgtac attaagtggg ggagaagcga cccgtctgac gattgctttg
     6361 ctttttacta agccaagtaa tgtgttgctg ttagatgaac cgactaattt tattgatatg
     6421 gcaacgatcg aagctttaga gaagctaatg caaatatatc cgggaacgat tttgtttact
     6481 tctcatgatt cttactttgt cgagcgtacg gctgatgaag tttatgaaat aaaagggcag
     6541 aaaataaaaa aagtacttac gagaaatttt taaatgtgct acgatgattt tcgtgacaaa
     6601 tttaaaggag tggtacagat tgcctacaaa taaaaaagaa ggaataattt ttacaacaat
     6661 gatgtgtttt ttgatggtct tggggatgag cgcgtataat ttatggctgc atcaagatct
     6721 tcatttatca agtttgttga ttggtctagt gcctggattc gtggtggctt tcattttgga
     6781 tgtcttcatc atcggtgtgc tggcgaaaaa aattgccttt aaactaccag taaatccaga
     6841 aagcaaggtc caaatgatct taactatttc atgcttgatg gttttaggaa tggtgacatg
     6901 tatgtcttta ttcggtgtga tcatggaaca aggaatacct gatcaattat tctctgctta
     6961 tatgaatgct tggaaaatga atgtgattgc cgctttaccg ttgcagttga tcatcgttgg
     7021 cccgctttca agaagtgtgc taaaagcaat tcaaagtaat gaatgatgga acactgttat
     7081 ttgaaaaaag gccttggcat gctttttgtc atagtctctt gaatgcagtt ctagaaaaat
     7141 agtgatccga acgataggtt ttggcctgtc gttcggatcg ctttttcttt tccaactgta
     7201 ttgctctttt attgaagata atttgctctt caatagatat ttctagaaag gttctgttta
     7261 tcccttactc aaaaaactgc tgcatttgtt ctcttacttg ctgacaccat tggagttcct
     7321 gctgacaaag cataattctg cgctggatca caacataacg tccaaattct tgtttcttgt
     7381 ctagcgagag gtcattcaat tttttttgat ttttttgcat gattttgttc agctgttgtt
     7441 ctctagtgaa tagcagtcca gtagctgttg ttttatcgag cgtcgaaaaa gcatagattt
     7501 ttgctagaaa ttcatctttt tgcgtaggag cgggagtttc ctctttgatc cattcctcta
     7561 caagagcacg tccttcatca gtcaagtggt agatcttttt ttgagagtgc tcgtctttgt
     7621 ccaaaacggt cacataaccc tgctggtcta acttggcaag cgttgtatag atctgactgt
     7681 gatgggcttc ccaaaaaatt gtgatcagct gcttcaattc atacccagaa cgtggtttgg
     7741 ataaaagcac gcataataat atataggcta aagtattcat aaaaatccta cctctcgtct
     7801 tattttcttt attatatcac agtaaagttt ccacgtttta catgtgcata ttgacatgta
     7861 aaataaatca tgtatgatgc taatagagag aaagggatgg aaatattgga gaatcaaata
     7921 gatatacaag aacaaacaaa tgaagaaaca aaggggaaaa gttcagcgct agttgtagca
     7981 gctacctgta tgatcgcttt tatgggagtg ggtctagtag acccaatctt aaaaacgatt
     8041 gctgcacagt taaacgctac gccagctgaa acgaccctgt tatttactag ttacatgtta
     8101 gtcactggtg tggtgatgct atttacaggt ttcttatcta gtcggatcgg tttgaaaaaa
     8161 acattgatga caggattgtt tatcatcgtt gtattcgctg gtcttggcgg attatctggg
     8221 aatatcggaa tgctgattgg gttacgtgcc ggctggggtg tcgggaatgc tttatttgtc
     8281 tctactgctt tagcaacgat cgtcagtatt ttagttggta acacagaaaa agcaatcatg
     8341 atgtatgaag cagcacttgg ctgcggaatg gctgttggcc ctctagtcgg tggggtcttg
     8401 ggcagtggtt catggcgtta tccatttttt ggggtagcat ttttgatgtt ttgcggtttt
     8461 atagccctag cggtactttt acctgaaact gagaaaccaa agagaaaagt aggcctttgg
     8521 gaaggtgtca aagcgttaag caatcataaa cttcgttcag ttggactaat cgcattgtta
     8581 tataatttcg gattctttac tttacttgcg tatgcgccat ttttattgat tggctattct
     8641 gaattagaag tcggt
//
LOCUS       DACECZ010000318         7417 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.guided.119, whole genome shotgun sequence.
ACCESSION   DACECZ010000318 DACECZ010000000
VERSION     DACECZ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.guided.119"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            396..1880
                     /gene="lsa"
                     /locus_tag="IWO35_002890"
     CDS             396..1880
                     /gene="lsa"
                     /locus_tag="IWO35_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0317865.1"
                     /translation="MSLINVSNLTFSYEGSYDNIFENVSFQIDTDWKLGFIGRNGRGK
                     TTFLNLLLGKYAYSGNISSTVKFEYFPYDVEDKSLYTIEVMKSICTEFMDWEIFREIS
                     LLDVQEDALYRPFNTLSNGEQTKVLLAALFLTASCFLLIDEPTNHLDIDARNVVQNYL
                     KRKKGFILVSHDRSLLDQCVDHILSINKTNIEIQKGNFTSWWENKTLQDNFELAENKK
                     LLKEIGRLSYAAKRSSNWSNKVEKSKYGTTNSGSKLDKGYVGHKAAKAMKRAKNIESR
                     HQEAVLQKSELLHNIEQYDDLKISPLEFHKECLIEANDLSLSYGDKEVCSNLNFRVNI
                     GDRVAIIGKNGSGKSSILKLINGDDIKFTGNFMLASGLKISYISQDTSYLKGNLSEFA
                     YNNKIDETLFKTILRKLDFNREQFDKNMVDFSAGQKKKVLIAKSLCESAHLYIWDEPL
                     NYIDIFSRIQIEKMILEYCPTLLFVEHDDAFCNNICTKNINLGL"
     gene            1934..2737
                     /gene="lnu(B)"
                     /locus_tag="IWO35_002891"
     CDS             1934..2737
                     /gene="lnu(B)"
                     /locus_tag="IWO35_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HAQ0317866.1"
                     /translation="MLKQKELIANVKNLTESDERITACMMYGSFTKGEGDQYSDIEFY
                     IFLKDSITSNFDSSNWLFDVAPYLMLYKNEYGTEVVIFDNLIRGEFHFLSEKDMNIIP
                     SFKDSGYIPDTKAMLIYDETGQLENYLSEISGARPNRLTEENANFLLCNFSNLWLMGI
                     NVLKRGEYARSLELLSQLQKNTLQLIRMAEKNADNWLNMSKNLEKEISLENYKKFAKT
                     TARLDKVELFEAYKNSLLLVMDLQSHLIEQYNLKVTHDILERLLNYISE"
     gene            3425..4246
                     /locus_tag="IWO35_002892"
                     /pseudo
     CDS             3425..4246
                     /locus_tag="IWO35_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            4389..4613
                     /locus_tag="IWO35_002893"
     CDS             4389..4613
                     /locus_tag="IWO35_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0317867.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGDEIKISNE"
     gene            4628..5497
                     /locus_tag="IWO35_002894"
     CDS             4628..5497
                     /locus_tag="IWO35_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ0317868.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5478..6212
                     /locus_tag="IWO35_002895"
     CDS             5478..6212
                     /locus_tag="IWO35_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0317869.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6245..7153
                     /locus_tag="IWO35_002896"
     CDS             6245..7153
                     /locus_tag="IWO35_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ0317870.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7150..7398
                     /locus_tag="IWO35_002897"
     CDS             7150..7398
                     /locus_tag="IWO35_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HAQ0317871.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
BASE COUNT         2587 a         1058 c         1531 g         2241 t
ORIGIN      
        1 aagggatttt attctttatt taaaactttg caacagaacc cttttcttgc taattatatg
       61 atgcataata agatatttaa agaaacggct atagaaccgt ttgttttgtt atgcaaattg
      121 caagaaagtt aagtattcta cactcaataa tacattacag taaccttatg ttatgtataa
      181 caaaaggtaa taatagtatt tgtttttgag ccgtaagaat ttactttctt gcggctttta
      241 atatttgtaa aagaaaggaa gataaacgtg atagacaatg atactatgtc attcatgaca
      301 aaatccttgc agagttggat aacattatag agtaattaat gccgaaaacc gactttgctt
      361 ataaaatttt tataatgtaa aggaggaaga aatatatgtc cttaataaat gtttcaaatc
      421 taactttttc atatgaagga agttatgaca atatttttga aaatgtaagt tttcagatag
      481 atacagattg gaaactcggt tttattggaa gaaacggacg cggtaaaact actttcttaa
      541 atttactgct tggcaaatat gcgtattccg gcaatataag ttctacagtt aagtttgagt
      601 attttcctta tgatgtggaa gataagagtc tatatacaat tgaagtaatg aagagtattt
      661 gtacggaatt tatggattgg gagatttttc gtgaaatatc attgcttgat gttcaagaag
      721 atgctttata tcgtccgttt aatacattgt caaatggtga gcaaacgaag gtccttcttg
      781 cagctttatt ccttacagcg agttgtttcc tgcttattga tgaacctaca aaccatcttg
      841 acatcgatgc acgtaatgta gtgcaaaact atttgaaacg caagaagggg tttattttgg
      901 tatctcatga tagaagctta cttgatcaat gtgttgacca tatactatct atcaataaaa
      961 cgaatatcga aatccaaaag ggaaatttta cttcttggtg ggagaacaaa acgttacaag
     1021 ataattttga actggcagaa aacaagaaac tccttaaaga aataggaagg ttgtcttatg
     1081 cagcaaaacg tagttcaaac tggtcaaata aagtagaaaa aagtaaatat ggaacaacaa
     1141 attctggttc aaaactggat aagggttatg ttggacataa ggctgcaaaa gcgatgaaac
     1201 gtgccaaaaa tattgagtca agacatcagg aagccgtttt acaaaaatca gaactgctcc
     1261 acaacattga acaatatgat gacttaaaaa tttcaccact tgaatttcac aaagagtgct
     1321 taatagaagc gaatgattta tcattgtctt atggagataa agaagtatgc agtaatctta
     1381 atttcagagt caatattggt gatagagttg ccattatcgg aaaaaatggg agtggtaagt
     1441 ctagtatcct aaaattgatt aatggagatg atattaaatt taccggaaat tttatgctag
     1501 caagtggact aaaaatttct tatatttcgc aagatacttc atatttaaaa ggtaatctat
     1561 ctgaatttgc ctataataat aagatcgatg aaactctatt taaaacgatt cttcgtaaac
     1621 tggattttaa tagagagcag tttgataaga acatggtgga ttttagtgct ggtcagaaaa
     1681 agaaagtact aattgctaaa agcctttgtg aaagtgcaca tttgtatata tgggatgagc
     1741 cattgaacta tattgatatt ttttcacgta tccaaattga aaaaatgatt ttggaatatt
     1801 gtcctacact attgtttgtg gagcatgatg atgctttttg caataacatt tgtacgaaaa
     1861 atattaattt aggtttgtag agattttgag ttgccacaat aactaaaaga tatttacatg
     1921 aaagggtgaa gaaatgttaa aacaaaaaga attaattgca aacgttaaga atcttactga
     1981 gtcagatgaa cgaattacag cttgtatgat gtatggatcg tttaccaaag gagaaggtga
     2041 ccaatactct gatatagagt tctatatatt tttgaaagat agtataacct cgaactttga
     2101 ttcatccaac tggttgtttg acgtagctcc gtacttgatg ctttataaaa atgagtacgg
     2161 aacagaggta gttatttttg ataatcttat acgtggggaa tttcatttcc tttctgaaaa
     2221 agatatgaac ataatcccct cgtttaaaga ttcaggttat attcctgata cgaaggctat
     2281 gcttatttac gatgaaacag ggcaattaga aaattattta tcagagataa gtggtgcaag
     2341 accaaataga cttactgaag aaaatgctaa ttttttgttg tgtaatttct ctaatctatg
     2401 gttgatggga atcaacgttc taaaaagagg agaatatgct cgttcattag aactcttatc
     2461 acaacttcaa aaaaatacac tacaacttat acgtatggca gaaaaaaatg ctgataattg
     2521 gctaaacatg agtaaaaacc ttgaaaaaga aattagcctt gaaaattata aaaaatttgc
     2581 aaagaccact gctcgattag ataaggtaga attatttgaa gcctataaaa attctttgct
     2641 attagttatg gatttgcaaa gtcaccttat tgaacaatac aacttaaaag ttacacatga
     2701 cattttagaa agattgttga attacattag tgaataggag agtaacgtta ttcacagtat
     2761 tgggtactga aaattaacag catacctcag tttttcaaaa aacagcgaag cctatggata
     2821 tgttcccgtc taccgatagt gcaaaaagtg tcgtggatat gtttccgaga acggacatac
     2881 acaaatgaca ttcattctgt cctctcaatc catgtggaag cagtatgtct gttaacaaga
     2941 aaataattat tgttcgtcct gacgagttgt tagactcgaa aaggataaac atcattttta
     3001 gaaccggtag ccgatttttg gctagcggtt ttttgggctc tttggcaaat agtggtgatt
     3061 gaatctcgcg ctatcctatg gaaaaccgcc ttgtgtgagg ggctgcgaga ccgcttcgat
     3121 cctagaattg ctgaagcacg aatctagcgt cactcacatt tcatcttttg acgtagctga
     3181 gacaaggtgt ttttcttcga tccgctagtg gttaatctct ttttggtgca aatatggttt
     3241 agaatttctt tgcatggctt aagggagtgg agaatcggat attagtagga tttacatcca
     3301 cagataaaaa taggcttgat tttaacagcg aaactaaaat accctactta aatgaagtga
     3361 tagattagat gttgggttgt taggagatgc ttaaaaatcc tatgcaatca atagttttta
     3421 gtgtttgttg acttgatccc taaatcgcct gagtactaaa cgttgagtgt gtctgcttac
     3481 tgtcactagc cagtaagtga gactgtagtt aatctctcat tttccccagt attatgttga
     3541 aaatagccat gaagcgatta tttctaaaga gttatttttg caggctcaag aagaaattta
     3601 tcggagaagt aatatctaca caggagaagg caagaacaaa agaatttata gtagcaagta
     3661 cgctttaagt gccatcacct tctgtggata ttgtggcgat atttatagga gaacctattg
     3721 gaatattcat ggcagaaaag aatttgtctg gcgatgcgtg actagaatcg agcagggtcc
     3781 cgaggtatgt aagaaccgaa ccgtaaaaga agatgaactc tatggtgcgg taatgaccgc
     3841 aattaataag ctacttgctg gtggcaacaa tatgataaag acactggaag aaaatattca
     3901 tgctgtgatt ggagaaacca cagaatacca aatttcagag attaataccc tactagatga
     3961 aaaacaaaaa gaactaatca agctggccaa taaaggtcaa gattatgaat acctggtaga
     4021 tgagatcgat gagatgcgag ataagcgtca gactctttta gtagaagatg cctccctcag
     4081 tggcgagaac gagcgcatca atgagttgat tgaatttatc cgcaagaaca aattccgcac
     4141 cttagaatat gatgataagc ttgtaaggaa gataatccag aacgttaagg tctatgaaga
     4201 ccacttcgtc atagccttca aaccaggtat cgaaatggaa atatgaaaac cagaagtaaa
     4261 tagcccatga ctctgaagta gagttgtggg ttttctttta attgctgatt ataataatat
     4321 tttaatgact attgcattta tcaaccaaat ggtttatgtt attcttagca taaacttgga
     4381 ggtgctgtat gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg
     4441 tttccgaact tgctagacgt attggccaga ctccacagaa ttttaataaa aaattacaac
     4501 gagaaacagt aaccttggat gagttgaagg ccattgctga tgtgctaggt gtcaagtttg
     4561 tgcaggcatt tattttacca gatggagatg aaataaaaat atctaacgaa taaaggagga
     4621 tgtttatatg gtaggtaatg ttattcaaat agtaacagag aaattatcat ctttgccttt
     4681 tatagaaggc attgtattag ggggctcacg tgcaagaagc acccatacag aaaattctga
     4741 tatagatatc ggaatctatt acaattcaga ctcatttgac ctgacagcga ttaatcaaat
     4801 tgctacagag ttggatgatg agaatagaaa caaccttgtt gtacctcccg gagcgtgggg
     4861 tgattgggtt aatggcggtg gatggttagt tataaacggg tgtcatgttg acttgatatt
     4921 acgtgatata aaacgggtgg aacaaataat caaagatacg gagcaaggaa ttgttactgc
     4981 caattatcag accgggcatc cccatggtta tataagtgct atgtatcgtg gagaattagc
     5041 gattagtaaa atactatatg ctaagaacga aagcttatgc gaattaaaaa agcaggcaga
     5101 aatttaccct actgctctaa agaaaagctt gatgaacttt tttatatttg aagctgaatt
     5161 ctctttaatg tttgtaaaag caaatgcggg ggcagaggat aaatattata ttgcaggcca
     5221 tgtttttcgt attatttcat gcttaaatca agtactattt gcatgtaata atgcttattg
     5281 cattaacgaa aagaaagcta taaaactgct tgaaactttt gaatataaac ctgaaaaata
     5341 tgccgaaaga gttaatcata tttttgaagt actcggtctt tctctttttg aatgctacga
     5401 catgaccgag aaactttata aagaagtgaa tgaaattgta tcggagataa ataacttttt
     5461 aaacgaggag agttcagatg aaagaaaaca aatatgatga taatatattt tttcaaaaat
     5521 acagtcaaat gagtcgctcg cagaaaggac tggctggtgc gggagaatgg gagactttga
     5581 aaaagatgct acctgatttt aagggtaagc gtgtgcttga tttaggatgc ggctatggat
     5641 ggcactgtat atatgcgatg gaaaacggtg cttcctctgt agtaggtgtt gatatttctc
     5701 ataaaatgct cgaagtagca aaaggaaaaa cccattttcc acagattgaa tatgaatgct
     5761 gtgccataga agatgtggat ttcccagagg agagctttga tgtaatacta agttcgcttg
     5821 cgtttcatta tgtagcagac tatgagaatt taataaaaaa gatatatagg atgctgaagg
     5881 ctggtggcaa tttagttttt acagttgaac atcctgtttt tactgctcat ggaacacaag
     5941 actggtatta taacgaaaaa ggagaaatac tgcatttccc ggtggacaat tattattatg
     6001 agggcaaacg gacagctatg tttttggaag aaaaggttac aaaatatcat agaacactga
     6061 ccacatatct aaatacactg ctttcaaata gttttataat aaatcagatt gtggagccac
     6121 agccgccaga gaacatgatg gatattccgg ggatggcgga tgaaatgcga cgcccaatga
     6181 tgctgattgt atcggcaaaa aagaagatgt aataatatag aaaaaataaa cgaggagtat
     6241 gtaaatgaga tcagaaaaag aaatgatgga tttagtactt tctttagcag aacaggatga
     6301 acgtattcga attgtgaccc ttgaggggtc acgcgcaaat attaatatac ctaaagatga
     6361 atttcaggat tatgatatta catattttgt aagtgatata gaaccgttta tatctaatga
     6421 tgactggctt aatcaatttg ggaatataat aatgatgcaa aagccggagg atatggaatt
     6481 attcccacct gaagaaaagg gattttccta tcttatgcta tttgatgatt acaataaaat
     6541 tgatcttacc ttattgccct tggaagagtt agataattac ctaaagggcg ataaattaat
     6601 aaaggttcta attgataaag attgtagaat taaaagggac atagttccga ctgatataga
     6661 ttatcatgta agaaagccaa gcgcaaggga gtatgatgat tgctgcaatg aattttggaa
     6721 tgtaacacct tatgttatta aaggattgtg ccgtaaggaa attttatttg ctattgatca
     6781 ttttaatcag attgttcgcc atgagctgct gagaatgata tcatggaagg tcggcatcga
     6841 aacaggcttt aaattaagtg taggcaagaa ctataagttt attgaaaggt atatatccga
     6901 ggatttgtgg gagaaacttt tgtccaccta ccggatggat tcctatgaaa acatatggga
     6961 agcattattt ctatgccatc aattgttcag ggcggtatcc ggtgaggtgg cggaaaggct
     7021 tcattatgcc tatccggagt atgataggaa tataacaaaa tataccaggg acatgtataa
     7081 aaaatacact ggtaaaaccg gctgcctgga tagcacatat gccgctgata tagaagagag
     7141 gcgggaacag tgattacaga aatgaaagca gggcacctga aagatatcga taaacccagc
     7201 gaaccatttg aggtgatagg taagattata ccgaggtatg aaaacgagaa ttggaccttt
     7261 acagaattac tctatgaagc gccatattta aaaagctacc aagacgaaga ggatgaagag
     7321 gatgaggagg cagattgcct tgaatatatt gacaatactg ataagataat atatctttta
     7381 acccgtggtg ctagttaatt ttaatatcct aaagaat
//
LOCUS       DACECZ010000319         7314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.guided.122, whole genome shotgun sequence.
ACCESSION   DACECZ010000319 DACECZ010000000
VERSION     DACECZ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.guided.122"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            160..1056
                     /locus_tag="IWO35_002898"
     CDS             160..1056
                     /locus_tag="IWO35_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ0317872.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1206..2147)
                     /locus_tag="IWO35_002899"
     CDS             complement(1206..2147)
                     /locus_tag="IWO35_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0317873.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2273..3394
                     /locus_tag="IWO35_002900"
     CDS             2273..3394
                     /locus_tag="IWO35_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0317874.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3652..5154
                     /locus_tag="IWO35_002901"
     CDS             3652..5154
                     /locus_tag="IWO35_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ0317875.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5221..5748
                     /locus_tag="IWO35_002902"
     CDS             5221..5748
                     /locus_tag="IWO35_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0317876.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5959..7020
                     /locus_tag="IWO35_002903"
     CDS             5959..7020
                     /locus_tag="IWO35_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0317877.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7023..>7314
                     /gene="murQ"
                     /locus_tag="IWO35_002904"
                     /gene_synonym="yfeU"
     CDS             7023..>7314
                     /gene="murQ"
                     /locus_tag="IWO35_002904"
                     /gene_synonym="yfeU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="catalyzes the cleavage of the lactyl ether moiety
                     of N-acetylmuramic acid-6-phosphate (MurNAc-6-P) to form
                     N-acetylglucosamine-6-phosphate (GlcNAc-6-P) and lactate;
                     involved in MurNAc dissimilation pathway; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0317878.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEM"
BASE COUNT         2439 a         1272 c         1551 g         2052 t
ORIGIN      
        1 aaaactttta ggcgaatttt aatagaagaa tgatttattt catcatattt tcatacaaat
       61 gttaaatttt catacaaata taggaaacgt tttactggct ttttactatt taaacaggct
      121 atcataggaa aaagtgacag agaaagtgag gcggaaaaga tgaacaaaaa tgacgtactg
      181 ctagttgcaa aaaataaatc actttatttt ttgccttcca tccgctcttt agatgtttgg
      241 tccggaaaag atttactaca gtcggaattc attaaatcag ctagacaaca gttggcaaat
      301 tatcagctga tcgttttttt agattatgga tttgatatgg agatggctcg acgcgtgcgc
      361 ccatttacca atgcaaaaat tgtgttattt ttttggaatc acttcaaagc agaacacgag
      421 gcgatgctga agcaggcaca agatgaaccg gcaatcgatg agatttatca ttttgatttt
      481 ttggaagcaa aagaacttgg gctgaaacat aatagttcat tttattcgaa acatatgaat
      541 ttttcttcta gcgaaccaaa gacggatcta ttttttggtg caacgaataa tgggagaaaa
      601 gaacgagcag aaagctataa gcaggaattt ataaaaagag atgttacgac caactatttc
      661 attcttccac atcgagggaa tgaacaacct ggatatctta cttatgaaga gtacctgaaa
      721 aaaacagggg aatcgcgcgg gattctggaa ctgttgcggg agggtcagca aggagtgact
      781 ttgcggacat ttgaatccat gtattttcaa aagaaactag tgacggataa tcaagcgatt
      841 gctcattatc atttttatcg tcctgaaaat atttttttgc ttcgagaacg tgatttagat
      901 gaattaccgg aatttctgca tacaccattt aagccaatcc tatcagagct attggaattt
      961 tatgatgcag aaaattgggc aaaacgattt atcactaacg attccactat ttatgagcaa
     1021 tacgaatatg acctctcctc tgttgaaaac aaataaaaaa cctacatttt gaagagatta
     1081 tgtactgaaa caaacaagca caattcttat ggcatcatga gagttgtgtt ttctttttga
     1141 tcttttattg ggcttaaaaa aagagaacca gctgctgatt cagctgattc tcatcagaga
     1201 gccttttatt ctgcgccaag gattccttct tttgcatgga ctaattgggt caaaaaggca
     1261 caatatggtg tagcaatatc atatttttgt ccttttcgcc aaatggcacc attgatgtag
     1321 tcgatttcag tcaaacgatg gtttttgatc aggtcttggt acatagatgg gtaatgcaac
     1381 ccgatgttgt caggatcata agtagaagca atatgttggt acacttcttc ttgatctagt
     1441 atgattcctt cttttgctgc gatagaagca aattcagata cgattgttcg aaccatgctc
     1501 tctgccgctt tttgcgcgcc tagttcagca atattacaat ccaaaatcgt gcataaacca
     1561 ttcaatgttc catttacaca agcttttcgc caaatggaat aacgaacgtt atggctatag
     1621 atcggattta aatttgcttc ttgaaagact tctacaactt tttttgtaaa tgcttcgcct
     1681 tctggttcca agttctctag ttcgatttct ccatcaccta acaatgtgac ttttccggga
     1741 ccagctagac cagctgtcca catcgtgatc ccaaataaga tatttttctt tggcacatat
     1801 ttttccagta catcttcatg acctaatcca tttagtaaac acaaaacata agtattgtca
     1861 gtaataatgg attggatcga acaaaacata tcatctaatt gattggcttt tgttaatgct
     1921 acgatcaagt ccacgtgttc gttgctttcg atgatttctt ctggtgaata gattggcagt
     1981 tttgcgacca cttctttacc gttaaagtca gcaatcaacc catttgtacg aatggcttca
     2041 atgtgagcag gccaacgatc gatcaagagg acctcatttc cgctttgatg tagcatcaat
     2101 ccgattcggc tgcccattgc tccggcccca gctattgcga ttttcatgtt ttttctctcc
     2161 tttattcaat gagttccctt ttagtatata gaaaaatgga agaaaatagc attgttttgt
     2221 ttgaaaaaaa tgaaaaaaag tgtcttactt aagaagaaag tgagggatac agatggatat
     2281 tttggcggca attgattcat ttaaaggaag tgcttcttca tttgaactaa atcaggcagt
     2341 tctaaatggc tgggaagatc gtaaaggaag aaaaatcaat gtgccgattg cagatggcgg
     2401 agaaaatact gcagaagcaa tctattacgc gctcagggga acatggcgta ccattgatgg
     2461 gatggatctc ttattccgtc agcgaaaaat cagctattta ttgacaactt ggcaaggaaa
     2521 aaacaccgca gtcatcgaat ctgcacaagc attggggtta gatctacttg ataaaccaac
     2581 ggatcaaact atccgacttg cttccagtta tgccttagga gaacttatta aagatgcact
     2641 gcttcaagaa gtagaacaaa ttattgtcac tttaggagga agcgggtgta gcgatggtgg
     2701 tctaggatta ctccaatcct tgggtgcttc tttagaaggg tacacagaag gaaatccttt
     2761 attatctact aaaaaacttc attttgatcg aagaaacgtt cttgtttcta aagtaaaatt
     2821 gattatcgct gcagatgtta ccaatccata tacaggtcaa caaggagctg cttggcaatt
     2881 tggaagacaa aaaggaggaa tagatacgac gctagctttc ttagataaac aagcaggaaa
     2941 gattgctcag caaataaaag atttctggga ggtggattta gatgatattc caggaagtgg
     3001 agcagcaggc ggtattggtg gagccctttt tttgttggga gcgaagatgg tttctggttt
     3061 cgatttagtc agccaaatca ctggattgga aaaaaagatc gaacaagcag atgtcatcat
     3121 tacaggagaa gggcggatcg atcagcaaac gatccacgga aaagttccat acggtgtagc
     3181 agtgcttgca aaaaaatatc gaaaaccaat aatcgctatt tgcggaagta gagaccaaga
     3241 gctagaagga ctttctgatt ttctcccaat tgtcttaagt attcatttgg gtccaatagg
     3301 tttagaagaa gcaatggaac acaggcaaac attagaaaaa acaaaaatct taggacagac
     3361 acttagcaga tttttcacca tcctcccgaa gtagttgaca aaatcatttt ctttatgcta
     3421 tcgtaatttc aaatatagga caaagtgagg gatccgtgca ttgaaaaatt aagtcgattg
     3481 atgaaagctg cattcagact gtaccttgtg gagacaagga agcagtgtgc agattttcgg
     3541 gattggcttg gttttttcga tgtatgggtc tttttgtgtg tatccaaccc ttgcatcctc
     3601 tttagccagt tctgaaaata ggactggtct ttttttacga ggtgatggaa aatgtctaaa
     3661 atcgaaataa aaaatctgac attcggctac gacagccaag gcacattatt atttgaacaa
     3721 gcaaatctaa attttgacac acaatggaaa ctaggactta tcggacgaaa cggtcgagga
     3781 aagacaactt tactgaatat tctacaaaac aaactacctt atcaagggca agtaatccat
     3841 cagcaagaat ttgcctattt cccgcaacag acaaaagata aagaacgttt aacctattac
     3901 gtgttaaatg atattacgga ttttgagata tgggaaatcg aaagagagct ccaattgatg
     3961 caaacagatc ctgaaatctt atggagagaa ttcagcacac tatcgggggg agagaagaca
     4021 aaagtcctac tggcactttt atttgtggat gacactcatt tcccgttaat cgatgaacca
     4081 acgaatcatt tggatatctc tggtagaaaa caagtagcgg cttatttgaa aaagaaaaaa
     4141 caaggcttca tcgtggtcag ccatgaccgg ggatttatcg atgaagtagt ggaccatgtt
     4201 ttagcaatcg aaaaaagtca actggaactt tatcaaggga atttctctat ctatgaagaa
     4261 cagaaaaaac ttcgtgatga atttgaaatg gctcaaaatg aaaaattgaa aaaagaagtc
     4321 agtaggctaa agaaaacagc agctgaaaaa gccgaatggt ctcgttcccg agaaggagat
     4381 aaaacaaaga aacaagtcgg attcatcgat actgaatcta gacgagtgaa taaaggagca
     4441 gtgggtgctg atgctgcacg gacgatgaaa cgatccaaag caatcgtgaa tcggatggag
     4501 acccagatca gcgagaaaga aaaactatta aaagatatcg aatatatcga ttctttgacg
     4561 atgaatagcc aagcgtctca ccataagcga cttttaagcg tagaagatct tcaattaggg
     4621 tatgaaaatc tgttattcga gccaattcat tttacaatcg agcctcatca gcgggtggcg
     4681 atttcaggtc ctaacggtgc aggaaagtca tccattatcc attatcttct gggggcattc
     4741 aacggcaagg ttataggaga aaaataccag ccaaaacatc tgagcattag ttatgcaagc
     4801 caaaattatg aagacaatcg aggaacgttg gcggaatttg cagagaaaaa ccaagtagac
     4861 taccaagcat ttttgaacaa cctccgaaag cttgggatgg aaagagatgt ttttcataac
     4921 aagatcgagc agatgagtat gggccaacgg aaaaaagtgg aattggctaa atctttatca
     4981 cagccagctg aactatatac atgggatgaa ccattgaatt atttggatgt cttcaatcaa
     5041 gaacaattag aacaactgat cttgaacgtg aaacctgcca tgttactagt ggaacatgat
     5101 caaaccttcc tggataaagt atctactgag attatttctc ttgagagaat ctaacccttg
     5161 taatctcaag aacaacaagg tatggttaag gggaactttg ccaacgaagg agagaatcac
     5221 atgctttatg taacgagaca cggagaaaca acttggaacg cacaaggatt agtttgcgga
     5281 cgggcagatg tgccgctgac tgaaaaagga cagatgcagg cacagaaact agcagaaaag
     5341 gtagtagatt tgcctgttcc aatcacgaaa atcatccatt cccctcttca acgagcaaga
     5401 gatacagcac aagcagttgc tgatcgtctc agcttgccgc tgaccgttga tgaacgattg
     5461 gtagaaatgg attttgggga ttatgatgga ctgccaagca aagatgagaa ctttcaaaaa
     5521 gcacgtttag cgttcgctgt acgattccca aatggggagt cagtcttaga tgtgtatgca
     5581 cggatcgttc ctttattgaa agaatgcatc gaagacgaag aaaatgtcta tctgttagtt
     5641 tgccataatg ccttgataag agtgatcaat gcttattttc atccgatgcc taatgagggg
     5701 ttctttacat tcatggtaga taatacagaa ttggtttctt atgagtaatt atgtttttgt
     5761 gttatttaaa taaaatattt caaggcactg cttttgctta gcagtgcctt ttttaggtta
     5821 tgacaattct tttgataata taaactcaac ctttagaaat gaaatttaat tattttaata
     5881 aaagtattga aattttgttt ctttagatat atactctagt catagaaatg aagacggata
     5941 cagaaaaaga gggataatat gtttggcttt tcaatattta tgaacgattc tttgtcagaa
     6001 gagaagaaac agtatatccg ccaaatggca tccaatggct ttgtcggtat tttcacttcc
     6061 atgcatatac cagaagatga tgtttcggca tatcaaaagc gattgatcga actaggaagc
     6121 gaagcgaaaa agaataaact agaattgatg gtcgatattt caggagaggc attggaacga
     6181 gcgggttttt cattagaaga tctaacgcct ttgaaagcaa ttggtgtaac aggtttaaga
     6241 atggattatc atatttccaa tcagcagatt gccgactggt cacatcaatt gaaaatcagt
     6301 ttgaatgcta gcacgatcac gcctaaagat atcgatgaat tgaaagaagc agaagctgat
     6361 ttctcacaga ttgaagcttg gcataattat tatccacgtc ccgaaacagg gctggacaaa
     6421 gaatggtatc agaaaaaaaa tcaatggctc aaaattcaag gtctccttgt ccaaggtttt
     6481 gtaccaggag atacagagtt gagaggccca ctatatcaag gattgccaac tttggaagaa
     6541 catcgaggga tccatcctct tgcaggcgct ctagatctat tagcaagtaa cacggatatt
     6601 gtttacatcg gagatgcagg tctgtcagaa aacgttcagg agcaatttgc ttctttccaa
     6661 aaagaacaga cagtcttgct ccatacggaa cctgtagatg aagaatttta cgaatatata
     6721 ttagggaagc atacgaatcg gcaagacgat gcaagagatg tcatacgaag cgtagatgca
     6781 cgttttcgag agattccgcc tatcccagca agaaatacag ctacgcgtat gaaagggtct
     6841 attactttag ataacgaaaa gtatttgcgc tatatgggtg agatccaatt aaccaaatat
     6901 gatttgccag cggatgaaaa agtgaatgta gtagctaaag ttatcaaaga agagctacca
     6961 ttgatcaacc aaatcaaagc tggtatgaac tatcaattta taagaaaaga aggaagataa
     7021 aaatgagcaa aatcaattta gagaacctaa caacggaacg cagaaaccaa gaaacttttg
     7081 gattagatga gatgagtatt tctcaagccg ttcaactaat gaacaaagaa gatcagaaag
     7141 tggccgctgc ggttgaaaaa gaactgacaa gtatcgaacc agtgattgct aacacgattg
     7201 agtcctttaa aaaaggtgga cgtctgattt atatgggagc cggaacaagt ggaagactag
     7261 gtgtgttaga tgctgctgaa tgcgtaccga cattcggtgt agaaccggaa atgg
//
LOCUS       DACECZ010000320         5438 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.guided.142, whole genome shotgun sequence.
ACCESSION   DACECZ010000320 DACECZ010000000
VERSION     DACECZ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.guided.142"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            19..546
                     /locus_tag="IWO35_002905"
     CDS             19..546
                     /locus_tag="IWO35_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0317879.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(609..1262)
                     /locus_tag="IWO35_002906"
     CDS             complement(609..1262)
                     /locus_tag="IWO35_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0317880.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            1394..2236
                     /locus_tag="IWO35_002907"
     CDS             1394..2236
                     /locus_tag="IWO35_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0317881.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            2394..3290
                     /locus_tag="IWO35_002908"
     CDS             2394..3290
                     /locus_tag="IWO35_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0317882.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            3293..4006
                     /locus_tag="IWO35_002909"
     CDS             3293..4006
                     /locus_tag="IWO35_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ0317883.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            4026..4544
                     /gene="msrA"
                     /locus_tag="IWO35_002910"
     CDS             4026..4544
                     /gene="msrA"
                     /locus_tag="IWO35_002910"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0317884.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            4541..4768
                     /locus_tag="IWO35_002911"
     CDS             4541..4768
                     /locus_tag="IWO35_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ0317885.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            4920..>5438
                     /gene="lepB"
                     /locus_tag="IWO35_002912"
     CDS             4920..>5438
                     /gene="lepB"
                     /locus_tag="IWO35_002912"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0317886.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVY"
BASE COUNT         1967 a          952 c         1064 g         1455 t
ORIGIN      
        1 atcagaaagg atgagaaaat gttagctgca atttgggcac aggatgaaaa cggattgatt
       61 ggaaaagaag accgtcttcc ttggcgtttg ccaaatgatc tgaaattctt caaacaaatg
      121 acagaagcaa atacactagt gatgggacga aaaacattcg aagggatggg tagccgtccg
      181 ttgccgaatc gtcagacgat tgttttgacg agagattctt cctataaagc agaaggcgtg
      241 catgtcatgc atagtgtgga agacgtgctt gctctggaaa aagaaacaga cggtatcttt
      301 ttcatcgctg gagggtcagc tgtttatcaa gaatttctgc cattttgtac gatattatac
      361 cgtacagtca tccatcatgt attcgatgga gatgcttatt ttccacctgt tgactggtct
      421 gattggtcac tgatcaatac tagtcaagga gaaatggatg aaaaaaatcg ttatccgcat
      481 caatttgaaa cgtatcagcg taatgagaat cctgtaaaaa gcgggacagg cgaattgacg
      541 atataaaaaa gctatggata gactagctgt tatttagagc tgtcccatcc atagctttat
      601 tttttttatt aagtaaagaa taaaacagag aagtacataa aacctgcacc taacataaca
      661 aacaaatgcc aaacaacatg catatagcgg atagaattca agctataaaa gaatgcgcct
      721 atcgtgtaag aaagcccgcc tgctaccatc aaaataacac cagtcattcc aagagagtga
      781 taaagaggaa cagcagccac gacacagagc caccccataa aaatatagat gatcgtagat
      841 acttttgata ctctctcccg tttatgcaac gtcaacgctt tatatataat gccaagaatc
      901 gcaaacaacc agattaatac aaataaagtc caacctagcc atcctcggat actcagcaaa
      961 caaaaaggag tataacttcc tgcaatcaaa agaaaaatcg aactatgatc gaatacttgg
     1021 aacacctttt ttgcttttgt aaaaatcaat gaatgaaaca aagtagacgt taaaaataaa
     1081 agaatcatca tcgatccata gatagcataa gagacaatat ggagggagga atgcaagtgg
     1141 gcacctttta ctaaaagaac cactagtccc gcgatactca atccagtccc aattccatgg
     1201 gtgactgcat taaacacttc atttaatatt tcatacgttc gattaaattt cgttttttcc
     1261 atgaaaacgg ctcctttact aggtgtaatt aatgaaaaat cattatttgt ctgttatact
     1321 aaaaatagaa attgtcttac atgtagttta acatgaataa ggcatcttgt taaagaaaga
     1381 gagagagaaa ataatgacaa atgtaaaaat cgtaacagat tcttcatgta cgatggaaaa
     1441 aaatgtacgc gatgctttag gcattcgtgt catgccgcta tccatcatga tcgacggaac
     1501 agtatatccc gatgatgacc aactagacgg cgaaagattt atggaaatga tggcgaaagc
     1561 aaaaaatctg cccaaaacaa gccaaccacc aattggagaa ttcatctcga tgtatgatga
     1621 gctaggagaa gacggcagtg aagtgatttc gatccatatg acaaaaggct tgagtggaac
     1681 agtagaagca gcaagacaag ccagtaatct aacaaaaacg aaagtaacgg ttatcgacag
     1741 tgataccact gaccaaggat tatcttttca ggtaattcga gcagctgaat tagcaaaaga
     1801 aggtaaaaca gtagagaaaa tccttgaaga agtcaaaaaa gtacaagaca atacaaaatt
     1861 atacatcgga atatccacac tagataactt ggtaaaaggt ggaagaatca gccgaacaac
     1921 tggtttgctg tctagtgttt tgaacatacg cgtggtgatg aatttcgccc attctgaatt
     1981 aatccctgta actaaggggc gaggaaagaa aacgttcgat aaatggttta ataatttgaa
     2041 aaatgaactg aaagaattgc caaatgtgcg acaaatcggt atttcacatg cggatgcaaa
     2101 agaattggcg caagaattca aagagggttt gcaagaatta ttcccggaca tgcatatccc
     2161 tgtactgcat acgaatccgg ttatcgctac gcatactggt aaagacgcgt ttgcaatcat
     2221 gtattatgta gattagtcga aaagaggata agccctcagc ctgaaaggct tgcagcttat
     2281 tctctttttc tttataaaat taggaaaata attgttttct tttttcataa agactacctt
     2341 tttgataaaa tttttgataa cattagaacc aatcgaatac aagaggtgaa aaaatgaaat
     2401 taacgaaacg acatttgatc ctcgttttgt tcccattatt aattgcgatc atcatctatg
     2461 caggtttagc tgcttctata ccgaaagctg gcccactaat gaaaccagta actactgtga
     2521 gagaagatcc aaataaaaaa gaaaaacttc actacatagc tatcggagat tcgctgactg
     2581 aaggagtagg agatcaaacc aagcaaggag gattcgttcc ccttgtcgca aatgatctaa
     2641 aagaccgtta taatttgacc gcggttgaaa cagaaaacta tggtgtaaat ggtgaacgaa
     2701 gtgaccagat cttaaagcga gtaaaaacaa aagaagaaat tcgaaacaat atcaaaacag
     2761 ctgattttat cacactgact gttggcggta atgatttgat gaaagttatc caaaataatt
     2821 tattcgggtt aacggtcaat tcctttaaaa agccactgaa aaactatcag gaaaatgtca
     2881 cagaactatt ggaagaattt cgatccctca ataaaaccgc acctatttac attttaggca
     2941 tctacaatcc attttatttg aactttcctg agatcactga catgcagaca attgttaaca
     3001 attggaacaa aggaacagaa acggtgacaa aaaaaatgcc gaactgctac tttatcccaa
     3061 tcaatgattt gctttatcaa ggattgaatc aggaagtagg tgtcacagaa gaaggaacag
     3121 atcaaacaac tagcacgaca gaaaatctaa atggcatcaa aaataatgtg ctctatgaag
     3181 aagatcattt ccatccaaat aatttaggtt atcaattgat ggcaaatgca gtgagagatc
     3241 agctgatcga gacacaaaac caatggttag agaaggagga tgatcaataa acatgaaaac
     3301 aagatctgac gtatcttcaa acaaaacaaa gaaaacaaat tactggaaat tcgccttcct
     3361 tatccttctt ggggtagtac ttggttcgac cgtattttta ggcagccgta tttttgctaa
     3421 tcgggaacca gaactcccag aaataccagc tcttacggaa agacaaggag atcctgtctt
     3481 aacgatcaat tcaaataaag aaaaagtcaa tcaaatcatc agctttttcc ttagtgagta
     3541 tcaaaaagat agtgatattg aatataaatt ttatttggaa aatgaagcac tactcaatgg
     3601 tactttcgaa gtactaggtt ttcctatcga tttctattta tattttgatc cgtatgtgat
     3661 ggaaaacgga aatgtccaac taaaagctag aagtctttct atcggaacat tgagtttgcc
     3721 aatcaaagat gtgatgaata tgatcaagag aaactataaa ttaccagatt ggatcgaagt
     3781 caatacagat gatctgacta tcatgcttag gttggatcaa ttccgaatgc aaaacgggat
     3841 gtatattaaa gcagataaaa tcgacttagt aaatgatgac attcggttta gtctatattt
     3901 gccaaaagat acctctgatg cttcaaacaa ctcaaatacc aacaaaacta gcaccagcag
     3961 tactgaaagc aagacaaaag aaaataaaaa atcatcggat gagtagtatt agagggggaa
     4021 tcataatgga acgagcaata tttgcaggag gctgcttctg gtgtatgatc cagcctttcg
     4081 acacgcttcc tggtatccat acagtgatgt caggatatac tggagggcat gtaccagatc
     4141 cgacttatga acaagtaaaa agccaaacga ctggacatac agaagcagtg gagattatct
     4201 atgatccaac attgatttca tatgaacaat tagtagaact ttactggcag caaactgatc
     4261 caactgacgc tttcggacaa ttcgaggatc gcggtgacaa ttatcgcccg gtgatctttt
     4321 atttgacaga agaacagcgg atcattgctg aaaaaagtaa gaaacagtta gctgaaagtg
     4381 gacgtttcga acagccaatc gttacaaaaa tcgaaccagc ccaaccattt tatctagcag
     4441 aagaagacca tcaagagtat tataaaaagc atcctgaaaa ttttaaaaga aaccatgcac
     4501 gccgggctgc ctttatcgaa gaaaattgga ggaacgaagg gtgaatcgaa gcttttttca
     4561 ttatttaatg acactgcgag gcggtggcac acatgatgct ttatctgttt tcgctacaga
     4621 agccggtaaa gacatccaat ttcctaagca cagctctagc tacgaagaaa tttctaatta
     4681 tctggaactg aatgtcgact atttgccgtc catggatatc tttgacgaag catgggaaaa
     4741 atatttagaa aataatcatg acatataaga gatagttttg tactttatga tcaaaaaagt
     4801 tttttgtcca atagcctaat tgaaagaatt atttttattt cagctatcta gtcgcatttt
     4861 tttgatattt ttcttataat aaagaaagaa tgaaaaaaag agggatggat tgatagagat
     4921 tgacaaaaaa acaacgctat attaatcgct tctggcttgt attcaaatac ttacttgttt
     4981 ctgtattcct tgcgttcatg ttgcggggat ttttattcat cccggtacca gtggaaggca
     5041 attcaatgga aaacgtgttg aagcaaggtg atatggttgt gatggaaaaa ttttcggaga
     5101 tcagacgatt tgatattgtt gtttttcaat tagcagatgg cacgatttat atcaaacgag
     5161 taatcggtct tccaggagaa aatgtcagct accaaaatga tcaattgaag atcaatggga
     5221 aagtagttaa ggaaccttat ctaactaaaa acctgaaatc agatcatgca aatgcatcct
     5281 atacgacgga ctttacacta caagagctaa cgggacaatc caagcttccg gaagataatt
     5341 attttgtact tggagataat cgaagagttt caaaagatag tcgttcgttt ggcacgatca
     5401 ataaaactga tattctaggt aaagcaagat ttgtctat
//
LOCUS       DACECZ010000321         4702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.guided.152, whole genome shotgun sequence.
ACCESSION   DACECZ010000321 DACECZ010000000
VERSION     DACECZ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.guided.152"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            668..1327
                     /locus_tag="IWO35_002913"
     CDS             668..1327
                     /locus_tag="IWO35_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAQ0317887.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            1330..2673
                     /gene="vanS"
                     /locus_tag="IWO35_002914"
     CDS             1330..2673
                     /gene="vanS"
                     /locus_tag="IWO35_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0317888.1"
                     /translation="MERKGIFIKVFSYTIIVLLLVVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQNKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            2844..3650
                     /gene="vanY"
                     /locus_tag="IWO35_002915"
     CDS             2844..3650
                     /gene="vanY"
                     /locus_tag="IWO35_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ0317889.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLRWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            3668..4495
                     /gene="vanW"
                     /locus_tag="IWO35_002916"
     CDS             3668..4495
                     /gene="vanW"
                     /locus_tag="IWO35_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAQ0317890.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            4492..>4702
                     /locus_tag="IWO35_002917"
     CDS             4492..>4702
                     /locus_tag="IWO35_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAQ0317891.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRK"
BASE COUNT         1392 a          959 c         1185 g         1166 t
ORIGIN      
        1 atggtgaagt ctccagacat tggataactg ctggtctaat gcgcctttga gtatggggtg
       61 attgatttta ataccgggga ggtacggctt tttgaggaaa atattctggg atttacattt
      121 ggcgattatt acatttcctg taactgcaag gacacggatg atgaagatat gaggaatata
      181 gacttgctga ttttctactg tcttgaacgg tattaggact gaatctgctg cgcggagtgc
      241 ggcaagagcg accggaggga gtacacacag gccctgcctg tgtgaaaagc cataccgcaa
      301 ggtgcattcg caaaaaagag gccgcgcatt ttgctgaggc ttggggtgtc cccaacaagc
      361 aaaacgagaa cgccgcaggc gggcaagtgg tggatcagaa tatccgtaaa ggatattctg
      421 atccattgct tgccagtatt atcgggatgt tttgacaacc gctgtaatcg aaacatccca
      481 aacattaaga aggtgttaat tattattatc ttgatttaat tcgtttaaac caataatata
      541 gatacataca ccatgaaaag aagcgagtat cccaatgacg gatgattaaa atccgaaaag
      601 aaatcgcttt gaaactacag ggaaactaca gactgttatg ttatcttctt aaaatggagg
      661 gatttttatg tcgatacgaa ttctacttgt cgaggatgat gatcatatct gtaatacagt
      721 aagggcgttt ctggctgagg caggatatca ggtggatgcc tgcacagatg gaaatgaggc
      781 atacaccaag ttttacgaaa acacttatca actggttatt cttgatatta tgctgcccgg
      841 tatgaacggg catgaacttt tgcgtgaatt tcgtgcgaaa aatgatactc ccattctgat
      901 gatgacagcc ctgtcggatg acgaaaacca aatccgggcg tttgatgcag aggcagacga
      961 ctatgtaaca aagccattca agatgcagat tttactaaag cgggtggaag ccctgctgcg
     1021 gcgcagcggt gcgctggcaa aggaaatccg tgtcggcagg ctgacacttc tgccggagga
     1081 tttcacggta ctttgtgacg gtacagagct gcccctgaca cgaaaagaat ttgaaatcct
     1141 tttgctgctg gtgcagaaca aaggcagaac cttaacgcat gaaatcattt tgtcacgtat
     1201 atggggatat gactttgagg gtgatggcag cacagtccac actcatatca aaaatttgcg
     1261 ggccaagctg ccggaaaata tcatcaaaac cattcgcggt gtaggatacc gattggagga
     1321 atcataataa tggaaagaaa agggattttc attaaggttt tttcctatac gatcattgtc
     1381 ctgctactgg ttgtcggtgt aacagcaaca ctgtttgcac agcaatttgt gtcttatttc
     1441 agagtgatgg aattacagca aacagtaaaa tcctatcagc cattggtgga actgattcag
     1501 aatagcgata ggcttgatat tcaagaggtg gcagggctgt ttcactacaa taaccaatcc
     1561 tttgagtttt atattgaaga taaagaggga agcgtactct atgccacacc aaatgccaat
     1621 acatcaaata gttttagacc cgactttctt tatgtggtac atagagatga taatatttcg
     1681 attgttgctc aaaacaaggc aggtgtggga ttgctttatc aagggctgac aattcgggga
     1741 attgttatga ttgcgataat ggttgtattc agccttttat gcgcgtatat ctttgcgcgg
     1801 caaatgacaa cgccgatcaa agccttagcg gacagtgcga ataaaatggc aaacctgaaa
     1861 gatgtaccgc cgccgctgga gcgaaaggat gagcttggcg cactggctca tgatatgcat
     1921 tccatgtatg tcaggctgaa agaaaccatc gcaaggctgg aggatgaaat cgcaagggaa
     1981 catgagttgg aggaaacaca gcgatatttc tttgcggcag cttctcatga gctaaaaacg
     2041 cccatcgcgg ctacaagcgt tctgttggag ggaatgcttg gaaatatcgg tgactacaaa
     2101 gatcattcta agtatctgcg cgaatgcatc aaaatgatgg ataggcaggg caaaatcatt
     2161 tccgaaatac tggagcttgt cagcctgaat gatgggagaa tcgtacccat agctgaaccg
     2221 ttggacatag ggcgcacggt tgccgagttg ctgcccgatt ttcaaacctt ggcagaggca
     2281 aacaaccagc ggttcgtcac agatattcca gccgggcaaa ttgtcctgtc cgatccgagg
     2341 ctgctccaaa aggcactatc caatgtcata ttgaatgcag ttcagaacac gccgcaggga
     2401 ggcgaggtac ggatatggag tgagcctggt gctgaaaaat gccgcctttt tgttttgaac
     2461 atgggcgttc acattgatga tactgcgctt ccaaggctgt tcaccccatt ctatcgcatt
     2521 gatcaggcgc gaagcagaaa aagtgggcga agcggtttag gacttgccat cgtacaaaaa
     2581 acgctggatg ccatgagcct ccagtatgcg ctggaaaaca cctcggatgg cgttttgttc
     2641 tggctggatt taccgctcac atcaacattg taaatattta aaacttaaat gattttgacc
     2701 gacaggtata accctgccgg tctttttttg ctgctacagg aaaactacag actggctaca
     2761 gggaaagtac agatgcgctt gccataataa caatcgtacc agccacaaat cgtagtttta
     2821 ttgcaaagga ggcattcgat caaatggaaa aaagcaacta tcattccaat gcggatcatc
     2881 acaaacggca tatgaaacaa tccgtggaaa agcgggcttt tctatgtgcg ttcattattt
     2941 cgttcacagt ctgcacgctg tttttgaggt ggagactggc ttccgtattg gaggcaacac
     3001 agataccgcc catccctgca actcatacag gcagcagcac tgacgtagtg gagaatttgg
     3061 aggaaaacgc tcttgccacc gccaaagaac agggagatga acaggaatgg agcctgattt
     3121 tagtgaacag gcagaacccc atccccgcac agtacgatgt ggagcttgag caactatcaa
     3181 atggtgagcg gatagatatt cggatttctc cctatcttca agatttgttt gatgccgcaa
     3241 gaactgatgg agtttacccg attgtcgcat ccggataccg aacaacagaa aaacagcaag
     3301 aaattatgga tgaaaaaatt gccgaatata aggcgaaagg ctacacctct gcacaggcta
     3361 aagcggaagc agaaacttgg gtggccgtgc cgggaacgag cgagcatcag cttggtcttg
     3421 ctgtggatat caatgcggac ggaattcatt caacaggcaa cgaggtttat agatggctgg
     3481 atgaaaacag ctatcgtttt ggttttattc gtcgctaccc accagacaag acagagataa
     3541 ccggtgtgag caacgagccg tggcattacc gatatgttgg catcgaagct gccacagaga
     3601 tgtacaacca aggggtttgc cttgaggaat atttaaaacc agaaaaatga gaaaaggatg
     3661 ttatgttatg gacagaaaaa gattgacaca gcgtttcccg ttcctgcttc caatgagacg
     3721 ggcgcagaga aaaatgtgct tttatgcggg aatgagattt gacggctgtc gctatgcgca
     3781 gacgataggg gaaaaatcgc tttcccattt gctctttgaa acggattgcg cattatacaa
     3841 ccacaatacc ggatttgaca tgatatacca ggaaaacaag gtgttcaact taaagctggc
     3901 agcaaagacc ttaaacggct tattgataag accgggggaa accttttctt tctggtggct
     3961 ggtacgccat gcggacaaag atacccccta taaagacggc cttacggtga ccaatggtaa
     4021 actcaccacc atgtcgggcg gcggtatgtg ccagatgagc aatttactat tttggatgtt
     4081 cctgcatacg ccattgacaa ttatccagcg cagaggtcac gaagtaaagg agtttccaga
     4141 gccaaacagt gacgagatta aaggggtgga tgcaaccatt ccagagggct ggattgattt
     4201 aaaagtgcga aacgataccg actgcaccta ccaaatatgg gtgaccttag atgatgagaa
     4261 aatcatcggt caggtgtccg ccgacaaaga gccccaagca ttatacaaaa ttacaaatgg
     4321 cagtatccag tatgtccgtg aaagtggcgg gatttatgaa tatgcccagg ttaaacggat
     4381 gcaagttgcc ttaggtaccg gggaaataat agattgcaag ctgctttata caaacaaatg
     4441 caaaatctgt tatcccctcc cggaaagtgt ggatattcag gaggagaacc aatgagaaaa
     4501 agtgtgggca ttactgtttt tggatgcgaa caggatgagg caaatgcttt ccgcgcttta
     4561 tcgccggatt ttcatattat ccctacgctg attagcgatg cgatatcggc agacaacgca
     4621 aaattggccg ctggcaatca atgcgttagc gtaggccata agtccgaggt ttccgaggcg
     4681 acaattcttg cgctgagaaa gg
//
LOCUS       DACECZ010000322         4616 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.guided.153, whole genome shotgun sequence.
ACCESSION   DACECZ010000322 DACECZ010000000
VERSION     DACECZ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.guided.153"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..476
                     /locus_tag="IWO35_002918"
     CDS             <1..476
                     /locus_tag="IWO35_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0317892.1"
                     /translation="LPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYK
                     AADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKI
                     EITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1112..1609
                     /gene="dfrG"
                     /locus_tag="IWO35_002919"
     CDS             1112..1609
                     /gene="dfrG"
                     /locus_tag="IWO35_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ0317893.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1681..3633)
                     /locus_tag="IWO35_002920"
     CDS             complement(1681..3633)
                     /locus_tag="IWO35_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ0317894.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3640..3936)
                     /locus_tag="IWO35_002921"
     CDS             complement(3640..3936)
                     /locus_tag="IWO35_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0317895.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4114..4616
                     /locus_tag="IWO35_002922"
                     /pseudo
     CDS             4114..4616
                     /locus_tag="IWO35_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1472 a          768 c          774 g         1602 t
ORIGIN      
        1 cactcccacg ttatacaaca tcagatatgg gaggattata cgattacgcc ccaacagaag
       61 cgataaaaga aaggcctcac ttagaaaaac atgaagtaaa agtaggagaa tcgtgggaaa
      121 aagggaagta taaagctgca gataataaca ttacttacac agtggcaagc aacaaaaagc
      181 aaaaagtgac agtaccagta tatgcttatt taggtacaca ggtaaaagta aacggtaaag
      241 tcgtaaatga ctcttataaa aaacatggct tagtgaatgt ggatgtaaat gctggagaaa
      301 ataagataga aattacaaca aaatatactc caactgctct catggcttta tggatcagta
      361 ttctgactta ttgtttagtg gtgtattcaa gttttaaacg atttgcacct aataagttga
      421 aagattctcg atatgtgact aaaaaggaga taaacaagaa gaatgaaaat aaataaaaaa
      481 gtagtaatat tgagtggatt aattatagtt agtagtatat attctttcat ctttttcaaa
      541 aatacacaga gtatttatac cactggagat ctttcttttc acttatctcg aatcaagggg
      601 ttatcgacta tttttaccag tccgattaat tatgagacat tcaattatac tggatatggt
      661 gtaaattatt tttatccctt tctgactttt tttccagctg taatgttata ttggatgaca
      721 aaaaatttga ttgtaagcta tattatttac gtttggttat tgaacttgtg tacaacactc
      781 gtagcttatc attatggcga gcggttttta aaacaaaaaa aagcggcatt tttattttaa
      841 gtagttcagt tttggagtac aaaatgacaa aatgtacttc aaaatggact acttttttca
      901 ttttaactta cataccaaat tacatacaaa atgatttata aaatgcaatt gaaaatgata
      961 attgaatgaa aatttgtgat tttgtggtat tatgataata taacttatag aaaaaattgc
     1021 ttattcaact aacgggcagg ataatgagaa aaggtttttt gatagaaata agaaattaga
     1081 tggtaaatta gttaaaaagg ctgtgttata aatgaaagtt tctttgattg ctgcgatgga
     1141 taagaataga gtgattggca aagagaatga cattccttgg aggattccca aggactggga
     1201 atatgttaaa aatactacaa agggacatcc gataatatta ggtaggaaga accttgaatc
     1261 aatcggaaga gccttacctg acagaagaaa tattattctg acgagagata aggggtttac
     1321 ctttaatggt tgtgaaattg ttcattcaat agaagatgtt tttgagttat gtaaaaacga
     1381 agaagaaatt tttattttcg gaggagaaca gatttataat ttgtttttcc cttatgttga
     1441 gaaaatgtac atcacaaaaa tacatcatga attcgaagga gatacttttt ttccagaagt
     1501 gaattatgag gaatggaatg aggtatttgc ccaaaaaggg ataaagaatg ataaaaatcc
     1561 gtataactac tattttcatg tatatgaaag aaaaaactta ttgagttaac gggtgcgtta
     1621 attgaagaag aacagtaaag ataaagcaca agagcttggg attactccaa gctctttttg
     1681 tcatttgaaa taaattatat ctttccaatc aaattgttta tctgtaatcc ctaatctttg
     1741 agcaggtgtc aaaacctttt tatccgctga tttaaaaggg cgacaaaagt tgtagtaagt
     1801 tcttagaatt gtaatcgcaa attgagcata cttcggattg aagttagcgt aaatatagct
     1861 cttcttatca ccacgagctg ttgttaatgg tctttcaaga gatgatatat aacgtcttat
     1921 ctgttgaata aagctattgg tcgaatggtc attaacattt aatagcatct ttgcgatttc
     1981 gtttggttcc agagcagata aatccgttct acaatcaact gaataaaatc ctttatcctt
     2041 agtggctaat ggatgtttga tagggttatc ggcatatttg cgatatcttt ctccatcttt
     2101 actaatagcc tcttcgtgga attgatgact tgttagtaat tcagttagat aatgagatgc
     2161 caaagtccgt agaaactttg ttttgaatcc tctgatatct ccccaatcga gcagttctgc
     2221 ttttgcttgt ccgaattctt ttaagcattg ttttctgctt ttcgtcttgt taacttggca
     2281 aataaaatga tgtgcatcgc tcaaccttaa ttctttcgtg aatactctat aaaaggcgtt
     2341 tctaatagag aagtcatcat cactaatcat tcgccattcg tcggcattaa ccatttgttt
     2401 aattaaccaa tagtgagcag ttgtagtata aggagcatca acatttaaac catcgacaaa
     2461 tctactccta ttagtaattt tccctaattc gtgaagataa gcattcctat tttcgctatc
     2521 gttagcggat ggttcttgtg gataataaga ccaatctaac ctatcatttt tacgactaaa
     2581 agtattgaga tggtcttctt tgaattttct tgtatcttca tttaattcat ccattgaaat
     2641 attccaatca taggcaacat cagaacggaa cacatatctt gataagacct cagcagatac
     2701 aactatatac gtttgcatat taaggtcttc aaaccccgta tatttctttg aaccttgtcc
     2761 tttcttacgg acgttattta ggtagtagtg catcttgtcc gtattaagcc acatctcgtt
     2821 aaatttcttt gtttgaaggg gttgtgtctc atatcgttct aaaaactcca aacaacgcct
     2881 atacagccac tccagcttat gatagtaggt tccaactcca attcctaaaa tatcacaagt
     2941 acgactgact gaaacccttc caacaaccat cttagcgaac attggaagaa tggtgttctt
     3001 ttgttggtga taagtagtgg tttcttctct ttttggtaga acattagtga acttcttaca
     3061 cgccttacat tgatagcgtt gagatttgcc cctacttttc ccccttttgt aaaattgctt
     3121 cggctcattg aatggggttg attcttcttc cgagcagcct tctttatgaa attgataatc
     3181 tggctcaaca tcttggatgc tattgatttc tataaggcgt ttaatttctt caacaacaga
     3241 ccaattagat aaaggggtta ctgtacagtt ttgcgataca cctctgccaa taggattgtc
     3301 attgcagtac atacctttat gacctttatc tttggaacta ccagtcattt tatatcgact
     3361 tggtttccct tttacatctt tgaatttgtg ctgttctttc ccaaaattac aacaaaacgg
     3421 attgatgcag taattatatt gaatgggata ttcagaaccg ttccatttaa aagacgttgg
     3481 caagaacaac attttgttat accttgttgc aaatcgtttt ggagttaata aatcatattg
     3541 tttagcccta tcttttaatt cttcgtttga aatcggaatt ttaacatcta caatttcatc
     3601 ttccttcgtg gctaatcttt taagcttggt cattgtctat caacccactc tatctttgat
     3661 acccattttt tcaacaatcc gcttattact tcgcttgttg gcaatccatt caataaactt
     3721 cttatcgtct aaaatgttca ataagaattc atccactgct tcgctttcag tacgctctgc
     3781 atattcagcg tattgcttaa ctatcaccct tacttgctct gatatttccc aatccacaga
     3841 cttagcgttc ttattttttg gctcaagaaa ttgcatagga aaatgcaccc ctttttgctt
     3901 agtaaattgt aaccatttta acacaccaat ttgcattcct acaataatta tttcacgttc
     3961 gcatttttat atacatttta acttacattt tgtggtgtaa attaacaaca aacgttgatt
     4021 taacaataaa aaacgagtat caaattcagt tgcaatttga tactcattta ggtttacatt
     4081 ttcacttttt gtactccaaa actgaactac ttatttttat ttagttgtct ctatattttc
     4141 tctgcttatc ggacagtaga catctactat cgttccgcaa ttgccgaagc tatcgcaata
     4201 acacttgtta tccctgtatt attttatgct tatcaaatca tctctggaaa agaagaaaag
     4261 tatccgtctg taaagctagc attgagtatg agtttgctag tgtacagcca tgtactatca
     4321 acattaatga gtacagcatt aattatcatt tttattttta ttcgtttggt ttctaaaggt
     4381 ttcaagaatg cagattttat tgctatattt aaaaaattat ttagtgcggc aggaatgaca
     4441 ttggttctga cgagtgcttt ttggtatccg atgtttgaac aaatgctcta ccaaaaaatc
     4501 aacaaaccta gcgtgacaaa tttatatgct catgcatcta atgtgttcga tagtctgact
     4561 gaagctatga acaacgactt aactacttac tcaatgggac tagttggtct gttatc
//
LOCUS       DACECZ010000323         3733 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.guided.170, whole genome shotgun sequence.
ACCESSION   DACECZ010000323 DACECZ010000000
VERSION     DACECZ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.guided.170"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..158
                     /locus_tag="IWO35_002923"
     CDS             <1..158
                     /locus_tag="IWO35_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0317896.1"
                     /translation="IDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLD
                     LPLTSTL"
     gene            329..1135
                     /gene="vanY"
                     /locus_tag="IWO35_002924"
     CDS             329..1135
                     /gene="vanY"
                     /locus_tag="IWO35_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ0317897.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLRWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            1153..1980
                     /gene="vanW"
                     /locus_tag="IWO35_002925"
     CDS             1153..1980
                     /gene="vanW"
                     /locus_tag="IWO35_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAQ0317898.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            1977..2948
                     /locus_tag="IWO35_002926"
     CDS             1977..2948
                     /locus_tag="IWO35_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAQ0317899.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2941..>3733
                     /locus_tag="IWO35_002927"
     CDS             2941..>3733
                     /locus_tag="IWO35_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAQ0317900.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIP"
BASE COUNT         1073 a          793 c          995 g          872 t
ORIGIN      
        1 gcattgatca ggcgcgaagc agaaaaagtg ggcgaagcgg tttaggactt gccatcgtac
       61 aaaaaacgct ggatgccatg agcctccagt atgcgctgga aaacacctcg gatggcgttt
      121 tgttctggct ggatttaccg ctcacatcaa cattgtaaat atttaaaact taaatgattt
      181 tgaccgacag gtataaccct gccggtcttt ttttgctgct acaggaaaac tacagactgg
      241 ctacagggaa agtacagatg cgcttgccat aataacaatc gtaccagcca caaatcgtag
      301 ttttattgca aaggaggcat tcgatcaaat ggaaaaaagc aactatcatt ccaatgcgga
      361 tcatcacaaa cggcatatga aacaatccgt ggaaaagcgg gcttttctat gtgcgttcat
      421 tatttcgttc acagtctgca cgctgttttt gaggtggaga ctggcttccg tattggaggc
      481 aacacagata ccgcccatcc ctgcaactca tacaggcagc agcactgacg tagtggagaa
      541 tttggaggaa aacgctcttg ccaccgccaa agaacaggga gatgaacagg aatggagcct
      601 gattttagtg aacaggcaga accccatccc cgcacagtac gatgtggagc ttgagcaact
      661 atcaaatggt gagcggatag atattcggat ttctccctat cttcaagatt tgtttgatgc
      721 cgcaagaact gatggagttt acccgattgt cgcatccgga taccgaacaa cagaaaaaca
      781 gcaagaaatt atggatgaaa aaattgccga atataaggcg aaaggctaca cctctgcaca
      841 ggctaaagcg gaagcagaaa cttgggtggc cgtgccggga acgagcgagc atcagcttgg
      901 tcttgctgtg gatatcaatg cggacggaat tcattcaaca ggcaacgagg tttatagatg
      961 gctggatgaa aacagctatc gttttggttt tattcgtcgc tacccaccag acaagacaga
     1021 gataaccggt gtgagcaacg agccgtggca ttaccgatat gttggcatcg aagctgccac
     1081 agagatgtac aaccaagggg tttgccttga ggaatattta aaaccagaaa aatgagaaaa
     1141 ggatgttatg ttatggacag aaaaagattg acacagcgtt tcccgttcct gcttccaatg
     1201 agacgggcgc agagaaaaat gtgcttttat gcgggaatga gatttgacgg ctgtcgctat
     1261 gcgcagacga taggggaaaa atcgctttcc catttgctct ttgaaacgga ttgcgcatta
     1321 tacaaccaca ataccggatt tgacatgata taccaggaaa acaaggtgtt caacttaaag
     1381 ctggcagcaa agaccttaaa cggcttattg ataagaccgg gggaaacctt ttctttctgg
     1441 tggctggtac gccatgcgga caaagatacc ccctataaag acggccttac ggtgaccaat
     1501 ggtaaactca ccaccatgtc gggcggcggt atgtgccaga tgagcaattt actattttgg
     1561 atgttcctgc atacgccatt gacaattatc cagcgcagag gtcacgaagt aaaggagttt
     1621 ccagagccaa acagtgacga gattaaaggg gtggatgcaa ccattccaga gggctggatt
     1681 gatttaaaag tgcgaaacga taccgactgc acctaccaaa tatgggtgac cttagatgat
     1741 gagaaaatca tcggtcaggt gtccgccgac aaagagcccc aagcattata caaaattaca
     1801 aatggcagta tccagtatgt ccgtgaaagt ggcgggattt atgaatatgc ccaggttaaa
     1861 cggatgcaag ttgccttagg taccggggaa ataatagatt gcaagctgct ttatacaaac
     1921 aaatgcaaaa tctgttatcc cctcccggaa agtgtggata ttcaggagga gaaccaatga
     1981 gaaaaagtgt gggcattact gtttttggat gcgaacagga tgaggcaaat gctttccgcg
     2041 ctttatcgcc ggattttcat attatcccta cgctgattag cgatgcgata tcggcagaca
     2101 acgcaaaatt ggccgctggc aatcaatgcg ttagcgtagg ccataagtcc gaggtttccg
     2161 aggcgacaat tcttgcgctg agaaaggtcg gggtaaaata catttctacc cgcagcatcg
     2221 gctgcgatca cattgatacg actgccgccg agagaatggg aatctcggtt ggcacggttg
     2281 cgtattcgcc ggacagcgtt gcggattatg ctttgatgct gatgctgatg gccatacggg
     2341 gtgcaaaacc caccatgcac gccgtggcgc aacaagattt cagattggat cgtatccggg
     2401 ggaaagaact gggggatatg actgtgggag ttattggaac cggccatatc gggcaagcgg
     2461 tcgtcaaaag gctgcgggga tttggatgcc atgtgctggc ctatgataac agccgaaaaa
     2521 tggatgcaga ttatgtccag cttgatgagc ttctaaaaaa cagcgatatt gttacgctcc
     2581 atgtgccgct ttgtgcggat acccgccatc tgatcggtca gaagcaaatt ggagagatga
     2641 agcaaggcgc atttttaatc aacaccgcgc gcggagcgct tgtcgatacc ggggcgctgg
     2701 tggaggcgtt ggaaagcgga aagctgggtg gtgcggcact ggatgtgttg gagggcgagg
     2761 atcagtttgt ttataccgat tgctcgcaga aagtgcttga ccatcccttt ttgtcgcagc
     2821 ttttaaggat gccgaatgtg atcatcacac cccatacggc gtactacact gagcgtgtgc
     2881 tgcaggatac tacagaaaaa acaatcagga attgtctcaa ttttgaaagg agtttacagc
     2941 atgaatagaa taaaagtcgc aatcatcttc ggcggttgct cggaggaaca tgatgtgtcg
     3001 gtaaaatccg caatagaaat tgctgcgaac attgatacgg aaaaattcga tccgcactac
     3061 atcggaatta caaaaaacgg tgtatggaag ctatgcaaga agccatgtac ggaatgggaa
     3121 gccgacagtc tccccgccat actctccccg gataggaaaa cgcatgggct gcttgtcatg
     3181 aaagaaagcg aatacgaaac acggcgtatt gatgtggctt tcccggtttt gcatggcaaa
     3241 ttcggggagg atggtgcgat acaggggctg tttgtattgt ctggtatccc ctatgtgggc
     3301 tgtgatattc aaagctccgc agcttgcatg gacaaatcac tggcctacat tcttacaaaa
     3361 aatgcgggca tcgccgttcc cgaatttcaa attattgata aaggtgacaa gccggaggcg
     3421 ggtgcgctta cctaccctgt ctttgtgaag ccggcacggt caggttcgtc ctttggcgta
     3481 accaaagtaa acggtacgga agaacttaac gctgcgatag aagcggcagg acaatatgat
     3541 ggaaaaatct taattgagca agcgatttcg ggctgtgagg tcgggtgtgc ggtcatgggg
     3601 aacgaggatg atttgattgt cggcgaagtg gatcaaatcc ggctgagcca cggtatcttc
     3661 cgcatccatc aggaaaacga gccggaaaaa ggctcagaaa atgcgatgat tacagttccc
     3721 gcagacattc cgg
//
LOCUS       DACECZ010000324         2889 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.guided.189, whole genome shotgun sequence.
ACCESSION   DACECZ010000324 DACECZ010000000
VERSION     DACECZ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.guided.189"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            70..1110
                     /locus_tag="IWO35_002928"
     CDS             70..1110
                     /locus_tag="IWO35_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HAQ0317901.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQ
                     LKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSR
                     IDKVRYMFNKIT"
     gene            1229..1396
                     /locus_tag="IWO35_002929"
     CDS             1229..1396
                     /locus_tag="IWO35_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0317902.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(1456..1809)
                     /locus_tag="IWO35_002930"
     CDS             complement(1456..1809)
                     /locus_tag="IWO35_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0317903.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSIM
                     ESLASGINEARNIED"
     gene            2314..2736
                     /locus_tag="IWO35_002931"
     CDS             2314..2736
                     /locus_tag="IWO35_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0317904.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            2733..>2889
                     /locus_tag="IWO35_002932"
     CDS             2733..>2889
                     /locus_tag="IWO35_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0317905.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSM"
BASE COUNT          961 a          465 c          558 g          905 t
ORIGIN      
        1 tatagtggag tactacattt acgagattcg gttagagtat cagaaaaaga aaaaataaaa
       61 gttacagaaa tgtatacttc aataaatggt gaattatgta agattgatag agcttattct
      121 ggagaaattg ttattttgca aaatgagttt ttgaagttaa atagtgttct tggagataca
      181 aaactattgc cacagagaaa aaagattgaa aatccgcacc ctctactaca aacaactgtt
      241 gaaccgagta aacctgaaca gagagaaatg ttgcttgatg cccttttgga aatctcagat
      301 agtgatccgc ttctacgata ttacgtggat tctacgacac atgaaattat actttctttc
      361 ttagggaaag tacaaatgga agtgattagt gcactgttgc aagaaaagta tcatgtggag
      421 atagaactaa aagagcctac agtcatttat atggagagac cgttaaaaaa tgcagaatat
      481 accattcaca tcgaagtgcc gccaaatcct ttctgggctt ccattggttt atctgtatca
      541 ccgcttccgt tgggaagtgg aatgcagtat gagagctcgg tttctcttgg atacttaaat
      601 caatcatttc aaaatgcagt tatggaaggg atacgctatg gttgtgaaca aggattgtat
      661 ggttggaatg tgacggactg taaaatctgt tttaagtatg gcttatacta tagccctgtt
      721 agtaccccag cagattttcg gatgcttgct cctattgtat tggaacaagt cttaaaaaaa
      781 gctggaacag aattgttaga gccatatctt agttttaaaa tttatgcgcc acaggaatat
      841 ctttcacgag catacaacga tgctcctaaa tattgtgcga acatcgtaga cactcaattg
      901 aaaaataatg aggtcattct tagtggagaa atccctgctc ggtgtattca agaatatcgt
      961 agtgatttaa ctttctttac aaatggacgt agtgtttgtt taacagagtt aaaagggtac
     1021 catgttacta ccggtgaacc tgtttgccag ccccgtcgtc caaatagtcg gatagataaa
     1081 gtacgatata tgttcaataa aataacttag tgtattttat gttgttatat aaatatggtt
     1141 tcttgttaaa taagatgaaa tattttttaa taaagatttg aattaaagtg taaaggagga
     1201 gatagttatt ataaactaca agtggatatt gtgtcctgta tgtggaaata aaacacgatt
     1261 aaagataagg gaagatactg aattaaaaaa attccccctc tattgtccga aatgcagaca
     1321 agaaaattta attgaaataa agcagttcaa agtaactgtg attacagagc cagacgcaaa
     1381 gacgcagagc cgataaaatg agattaatac aatctcattt tatcggctct ttccgttatg
     1441 tatggattct tttaattagt cttcgatgtt tcttgcttcg ttgataccgc tggctaaaga
     1501 ttccattatg gatagttctt tgtctgtaaa gctatccatg tatttctcta tctgtaatcg
     1561 tcgggtgctt tttaccaagt tattagcagg taagaaaaat tcatcaacgg aaacatgaag
     1621 taacgataca aggtcataaa gaacttgtat gctggggtgt tgccctttat tttcaatatt
     1681 agttaagtac cgtgggtcaa tttcaatcaa tgctcccact tgttcacgag ttaaacctcg
     1741 tttcaatcga gcttctttaa tggctaaacc aaaggctcta aaatcatatt tatcttcttt
     1801 tttacgcata gtagaccacc tctatacatt ttattgttcc tactgaatta aaaacaggta
     1861 tagaaaaacg tgttatatgg tttataggtt tatatttaat aaaaagcact actaaacgcc
     1921 aataaaaaaa accgttatat ggtagtgcta tttacgctgt taaaatattg tatattactt
     1981 ccaaatggcg gtttgttgga ggtcaacgtc gccatgaagt acatcatata caataaattt
     2041 ccttacattg ggttcttgtc aaaaaaagtc gtctatctgc aatagataag tacgtccacc
     2101 aatgtggttt tataaatcat atagatagaa taacagaagc atgtaaacag agaaataaat
     2161 ctgtttatat gcttttttgg ctattcagaa cttttttaca aagtttattt atcagtaatg
     2221 caacaaatcc ccctttcaca ttgggactaa gagtgaaagg agataaacga gcaaggctca
     2281 cttcctttcc tagacagaaa gggggtgaga aacatgaaac catcttcttt tcagaccaca
     2341 atagaaaatc agtttgacta tatctgtaaa cgtgctatgg aagacgagcg aaagaattat
     2401 atgctttatc tttcaaggat tgcaaagcgt gaggtgtcct tttcggatgt tggcgattat
     2461 cttgttagcc agtttgcgac aacagataac tattcaactg actttcagat ttttacactc
     2521 aatgggttat cagtaggcgt tgaaaatgat ttgttgagtg aagcattacg tgagttgcca
     2581 gacaagaaac gtgaaattct actgctgttt tactttatgg acatgagcga ttcagaaatt
     2641 gcagacctgt tgaaattgaa ccgttctact gtctatcggc atagaaccag tggactagcc
     2701 ttaattaaaa agtttatgga ggaatttgaa gaatgaaaac acaatatcct atgattccct
     2761 ttcctctcat tgtaaaggca acagatggcg ataccgaagc gattaaccag attctacatc
     2821 attacagagg gtacataacg aagcgttccc tacgacttat gaaagatgaa tatggcaatc
     2881 aaagtatgg
//
LOCUS       DACECZ010000325         2755 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.guided.192, whole genome shotgun sequence.
ACCESSION   DACECZ010000325 DACECZ010000000
VERSION     DACECZ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.guided.192"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..528
                     /locus_tag="IWO35_002933"
     CDS             <1..528
                     /locus_tag="IWO35_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAQ0317906.1"
                     /translation="TVGVIGTGHIGQAVVKRLRGFGCHVLAYDNSRKMDADYVQLDEL
                     LKNSDIVTLHVPLCADTRHLIGQKQIGEMKQGAFLINTARGALVDTGALVEALESGKL
                     GGAALDVLEGEDQFVYTDCSQKVLDHPFLSQLLRMPNVIITPHTAYYTERVLQDTTEK
                     TIRNCLNFERSLQHE"
     gene            521..1549
                     /gene="vanB"
                     /locus_tag="IWO35_002934"
     CDS             521..1549
                     /gene="vanB"
                     /locus_tag="IWO35_002934"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAQ0317907.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            1555..2163
                     /locus_tag="IWO35_002935"
     CDS             1555..2163
                     /locus_tag="IWO35_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAQ0317908.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT          734 a          579 c          752 g          690 t
ORIGIN      
        1 actgtgggag ttattggaac cggccatatc gggcaagcgg tcgtcaaaag gctgcgggga
       61 tttggatgcc atgtgctggc ctatgataac agccgaaaaa tggatgcaga ttatgtccag
      121 cttgatgagc ttctaaaaaa cagcgatatt gttacgctcc atgtgccgct ttgtgcggat
      181 acccgccatc tgatcggtca gaagcaaatt ggagagatga agcaaggcgc atttttaatc
      241 aacaccgcgc gcggagcgct tgtcgatacc ggggcgctgg tggaggcgtt ggaaagcgga
      301 aagctgggtg gtgcggcact ggatgtgttg gagggcgagg atcagtttgt ttataccgat
      361 tgctcgcaga aagtgcttga ccatcccttt ttgtcgcagc ttttaaggat gccgaatgtg
      421 atcatcacac cccatacggc gtactacact gagcgtgtgc tgcaggatac tacagaaaaa
      481 acaatcagga attgtctcaa ttttgaaagg agtttacagc atgaatagaa taaaagtcgc
      541 aatcatcttc ggcggttgct cggaggaaca tgatgtgtcg gtaaaatccg caatagaaat
      601 tgctgcgaac attgatacgg aaaaattcga tccgcactac atcggaatta caaaaaacgg
      661 tgtatggaag ctatgcaaga agccatgtac ggaatgggaa gccgacagtc tccccgccat
      721 actctccccg gataggaaaa cgcatgggct gcttgtcatg aaagaaagcg aatacgaaac
      781 acggcgtatt gatgtggctt tcccggtttt gcatggcaaa ttcggggagg atggtgcgat
      841 acaggggctg tttgtattgt ctggtatccc ctatgtgggc tgtgatattc aaagctccgc
      901 agcttgcatg gacaaatcac tggcctacat tcttacaaaa aatgcgggca tcgccgttcc
      961 cgaatttcaa attattgata aaggtgacaa gccggaggcg ggtgcgctta cctaccctgt
     1021 ctttgtgaag ccggcacggt caggttcgtc ctttggcgta accaaagtaa acggtacgga
     1081 agaacttaac gctgcgatag aagcggcagg acaatatgat ggaaaaatct taattgagca
     1141 agcgatttcg ggctgtgagg tcgggtgtgc ggtcatgggg aacgaggatg atttgattgt
     1201 cggcgaagtg gatcaaatcc ggctgagcca cggtatcttc cgcatccatc aggaaaacga
     1261 gccggaaaaa ggctcagaaa atgcgatgat tacagttccc gcagacattc cggtcgagga
     1321 acgaaatcgg gtgcaggaaa cggcaaagaa agtatatcgg gtgcttggat gcagagggct
     1381 tgcccgtgtt gatctttttt tgcaggagga tggcggcatc gttctaaatg aggtcaatac
     1441 catgcccggt tttacatcgt acagccgcta cccacgtatg gtggccgccg caggaatcac
     1501 gcttcctgca ctgattgaca gcctgattac attggcgtta aagaggtgat aagtatggaa
     1561 aatggttttt tgtttttaga tgaaatgttg catggcgtcc gttgggatgc caagtacgcc
     1621 acatgggata acttcacagg aaaaccggtg gatgggtatg aggtgaatcg catcatcggc
     1681 acaaaggccg tggcgtttgc tctgcgcgag gcacaaatcc atgcggctgc gcttggctat
     1741 ggcttgcttt tatgggatgg atatcggcca agaactgcgg tggactgctt cctgcgttgg
     1801 gcagcgcaac cggaggacaa tctcacaaaa gaaaaatttt accccaatat agagcgagcc
     1861 gagttgatta caaagggtta tgtggcctca caatccagcc atagccgtgg aagcgcaatt
     1921 gatcttacgc tctaccacct ggatacaggg gaacttgttt caatgggaag taacttcgat
     1981 tttatggacg aacggtcgca tcatacagca aaagggatag gggatgcaga ggcacaaaat
     2041 cgaagatgct tgcgtaaaat catggaaagc agcggatttc agtcttatcg ctttgaatgg
     2101 tggcactata agttgattga tgagccatac cccgatacct attttaattt tgccgtttca
     2161 taatgaaagt attggttttg tatcagattt tggatttgat aaaatctatg ctggtcatgg
     2221 acaatctttt actggaaagt ggttaaagca tcttttgtaa tacattgcaa attatagtat
     2281 tcaatctgcc cagcggcaga aaagaaaaaa accgctgttg ggcagacctt tcccacgccc
     2341 attttgtatt ttgctggcgg cggttttgag aaatcaaggc cgccgttctt ttctggcagc
     2401 aaaaggtgtg gcggcccata ggaggatgcc gctatgctgt tttgctattg ccatgctcta
     2461 atccacttcc accactcaaa aaagccttgc ccctccacag ggctattccg gttatgagta
     2521 tgtactatac catgtaaatc agcagcatag caaagcattt tctcgcccgg taggtttgcg
     2581 acctgccggg cgatttttgt cgtcttttgc ggcccggctg tccccaaaaa aattttttga
     2641 gaaattttca aaaaggaggt atttagcggg tagctggcgg ccttgtcaaa gtcttgttag
     2701 cggaaaggga gagaaccacc taagtccccc acagaaaggg ggtgagaaga tggat
//
LOCUS       DACECZ010000326         2477 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.guided.201, whole genome shotgun sequence.
ACCESSION   DACECZ010000326 DACECZ010000000
VERSION     DACECZ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.guided.201"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            232..705
                     /gene="dfrF"
                     /locus_tag="IWO35_002936"
     CDS             232..705
                     /gene="dfrF"
                     /locus_tag="IWO35_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ0317909.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            832..1488
                     /locus_tag="IWO35_002937"
     CDS             832..1488
                     /locus_tag="IWO35_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ0317910.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            1670..2200
                     /locus_tag="IWO35_002938"
     CDS             1670..2200
                     /locus_tag="IWO35_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0317911.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
BASE COUNT          904 a          317 c          521 g          735 t
ORIGIN      
        1 gcttgttgcg tgagaaatgc tgaatatcac cctttacaaa acataggctg ataagtaact
       61 tttcaaaagc agaaaagaaa tggcaatgct tataacaata tgatataatg cagatgaaga
      121 caaaaaagga atttgtcgag ctggttagtt cgagataatt ttgagtttgt taagatgtgg
      181 tgcacttgat ggaatcacag gtacgacatc atattatgga ggatgaaata gatgataggt
      241 ttgattgttg cgaggtcaaa gaataatgtt ataggcaaga atggtaatat accatggaaa
      301 ataaagggag aacaaaagca atttagagag ttaacaacgg gtaatgtggt tattatgggg
      361 cgaaagtctt atgaagaaat cggtcatccg ttgcctaata gaatgaatat tgttgtttcc
      421 accacaacag agtatcaagg agataattta gtttcagtta aatcattaga agatgcatta
      481 ttattggcta aaggacgaga tgtatacata tctggtggat atggactatt taaggaagct
      541 ttgcaaatag tagataaaat gtatatcaca gaagtagatt taaatattga agatggagat
      601 acattctttc cagaatttga tatcaatgat tttgaagttt tgatagggga aacacttggt
      661 gaggaagtga aatatacgag aacattttat gtaaggaaaa aatgaattga gtaaattttg
      721 gatttagggt gttttcataa atatattgct ttttatgcat atataaattg ttgtaagaca
      781 aattccagtt tgtatggggc gataaaatca gaatttgaga gaggattgaa tatgaataag
      841 gaatggtctg aacttaataa aacaatgcag gcacaaataa aaaagaaaga tacttataag
      901 aggggtattg atactttact tactttacga agtcagttaa tacaaacctt agtatctttc
      961 aaagaagaat tatgcagaga agattttaac tcaatcccct tcataaacgc tgatggttat
     1021 catagtaaga cgattgctta ttctatttgg catattttcc gcattgaaga tatagttgtg
     1081 catacagtga taaatgaaga tgaacaagta ttttttgcag gcaattatca agagcgtatc
     1141 aattcaccta tcattacaac aggaaatgaa ctcatgaaac agcagattgc agacttttca
     1201 aaacagctta atctggaaga attatattta tatattttcg aagtatggga aagcactgag
     1261 aaaatgcttg aacggttatc ttatgatgaa ttgaaaagaa aaataccgaa agaaagaaaa
     1321 ggatacttag aatcgttgaa tgtagtaaac gacaatgaaa aagcaatttg gctgattgat
     1381 tattggtgta ataaagatat ttgtggacta attcaaatgc cgttttcaag acattggatt
     1441 atgcacacag aagcctgttt gcgtataaag aacaagatac attcataggc aaaaatttca
     1501 gtttgtagaa ctaaggaaat tggaatttaa ggaggaataa agtatggtta gtgattttat
     1561 aagagcagca tttccatgga ttttaatggg tttatttgta gcaatcagtt gttcccttat
     1621 gagtaaaaaa gagaaataaa ttcctgtttg tatttataaa agagaaaaaa tgagaaaagt
     1681 agttttattt attgccatga gccttgacgg atatattgcg gacggtaatg gtggagtagc
     1741 ttggctaaat ggtcatggaa atgacaatga aaacatcgac acttatacag aatttaccaa
     1801 agatatagat actgtcttaa tggggtggaa tacttctcat caggttgtta ctgaactttc
     1861 tccgcaagaa tgggtatata ataaatttac aacttatgta ttaacacata aagagtgcaa
     1921 ttcttcccaa caaattcatt ttacaagtga aaatcctgtt ttattattgg aacggctaaa
     1981 acaagaagca ggaaaggata tttggatttg tggcggagca aatcttgttc agcagttggt
     2041 aagcaaaaat atgattgata aatattacat ttctgttatt cctactctgt taggaaatgg
     2101 ggttcgtctt ttgggcaaca tagacaggga aataaagtta aggctttgta aaacacagac
     2161 ttataacgga ataaccgatt tgatttatat gcgtagataa ctagagagaa acagaaaagc
     2221 tgttaaaccg acggagcgat ccgaccacgt tggcgggaaa atccttaaga tttttccgcc
     2281 atgcttgcaa gggggcggtt cgcttcggcg gttcggcgag cttgcgagcc gttcaaagcc
     2341 cccgttatct aacaaggggg gcacaaaaaa caagaaacac tatacactaa caagcggaaa
     2401 acgctgtatt tataaggcaa aaccccattt ttacagtagt gatataaatt gtacctatat
     2461 cactatgaaa attgaat
//
LOCUS       DACECZ010000327         1504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.guided.235, whole genome shotgun sequence.
ACCESSION   DACECZ010000327 DACECZ010000000
VERSION     DACECZ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.guided.235"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            262..373
                     /locus_tag="IWO35_002939"
                     /pseudo
     CDS             262..373
                     /locus_tag="IWO35_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="IWO35_002940"
     CDS             394..477
                     /locus_tag="IWO35_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAQ0317912.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="IWO35_002941"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="IWO35_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0317913.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DACECZ010000328         1349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.guided.244, whole genome shotgun sequence.
ACCESSION   DACECZ010000328 DACECZ010000000
VERSION     DACECZ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.guided.244"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            46..297
                     /locus_tag="IWO35_002942"
                     /pseudo
     CDS             46..297
                     /locus_tag="IWO35_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="IWO35_002943"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="IWO35_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ0317914.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
LOCUS       DACECZ010000329          854 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51001_2017
            SAMN12640094-rid9879853.guided.280, whole genome shotgun sequence.
ACCESSION   DACECZ010000329 DACECZ010000000
VERSION     DACECZ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640094
            Sequence Read Archive: SRR10023223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741748.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,944
            CDSs (total)                      :: 2,884
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 36 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 13 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640094-rid9879853.guided.280"
                     /isolate="E51001_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            241..789
                     /gene="aac(6')"
                     /locus_tag="IWO35_002944"
     CDS             241..789
                     /gene="aac(6')"
                     /locus_tag="IWO35_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0317915.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          306 a          126 c          180 g          242 t
ORIGIN      
        1 ttttttaaag caaaagataa tctatatcat gatgaacaaa cagagttttt tcctccaatt
       61 gatggctttc ctgatctttc ctattttgaa taaagtgttt ctgtataatt tttttatttt
      121 ataagtaaga taaataatga aagaatagag atactgattg gtagctaaag atttttgagc
      181 gagaacattt cgttttttca aaaggactgt tagaataagc aagaacaagg gggaagacgt
      241 atgataatca gtgaatttga ccgtaataat ccagtattga aagatcagct ttctgattta
      301 ctgagactga cttggccgga agaatatgga gacagctcgg cagaagaagt agaagaaatg
      361 atgaatccag aacgaatcgc ggtagcagcg gtagaccaag atgagttagt aggatttatt
      421 ggtgcaatcc ctcaatacgg tatcacaggt tgggaattgc atccattagt tgtagaaagc
      481 tcccgacgaa agaaccaaat aggtactcga ttagtcaatt acttagaaaa agaagtagct
      541 tccagaggag gaatcacgat ttatttaggt acggatgatt tagaccatgg aacaacgtta
      601 agtcaaacgg acctgtatga gcatacattt gataaagtgg cttccatcca gaaccttcgt
      661 gaacatccgt atgaattcta tgaaaaatta ggttataaaa tcgtaggtgt cttaccaaat
      721 gcaaatggct gggacaaacc ggatatttgg atggcaaaaa cgattattcc tcgaccaaat
      781 tctcaataat aaaaagaggt tgtgacagaa gtaatcagct tcaagaaata agaaggaatt
      841 tacgaaaatt actt
//
